LOCUS       DANKHL010000001        55829 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000001 DANKHL010000000
VERSION     DANKHL010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.001"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            28..546
                     /locus_tag="PW101_000001"
     CDS             28..546
                     /locus_tag="PW101_000001"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2512438.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            674..1357
                     /locus_tag="PW101_000002"
     CDS             674..1357
                     /locus_tag="PW101_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDL2512439.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1666..1739
                     /locus_tag="PW101_000003"
     tRNA            1666..1739
                     /locus_tag="PW101_000003"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1700..1702,aa:Arg,seq:tct)
     gene            1898..2257
                     /locus_tag="PW101_000004"
     CDS             1898..2257
                     /locus_tag="PW101_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HDL2512440.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2380..4011
                     /locus_tag="PW101_000005"
     CDS             2380..4011
                     /locus_tag="PW101_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HDL2512441.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4073..4714)
                     /locus_tag="PW101_000006"
     CDS             complement(4073..4714)
                     /locus_tag="PW101_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL2512442.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4900..4976
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4996..5304
                     /gene="rplU"
                     /locus_tag="PW101_000007"
     CDS             4996..5304
                     /gene="rplU"
                     /locus_tag="PW101_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDL2512443.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5331..5675
                     /locus_tag="PW101_000008"
     CDS             5331..5675
                     /locus_tag="PW101_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDL2512444.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5688..5981
                     /gene="rpmA"
                     /locus_tag="PW101_000009"
     CDS             5688..5981
                     /gene="rpmA"
                     /locus_tag="PW101_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDL2512445.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6415..9222
                     /locus_tag="PW101_000010"
     CDS             6415..9222
                     /locus_tag="PW101_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2512446.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9413..9904
                     /locus_tag="PW101_000011"
     CDS             9413..9904
                     /locus_tag="PW101_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2512447.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10007..10996
                     /gene="manX"
                     /locus_tag="PW101_000012"
     CDS             10007..10996
                     /gene="manX"
                     /locus_tag="PW101_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDL2512448.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11029..11832
                     /locus_tag="PW101_000013"
     CDS             11029..11832
                     /locus_tag="PW101_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDL2512449.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11853..12767
                     /locus_tag="PW101_000014"
     CDS             11853..12767
                     /locus_tag="PW101_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDL2512450.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12850..13224
                     /locus_tag="PW101_000015"
     CDS             12850..13224
                     /locus_tag="PW101_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HDL2512451.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13363..13608
                     /locus_tag="PW101_000016"
     CDS             13363..13608
                     /locus_tag="PW101_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2512452.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13780..14844
                     /locus_tag="PW101_000017"
     CDS             13780..14844
                     /locus_tag="PW101_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL2512453.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15090..15515
                     /locus_tag="PW101_000018"
     CDS             15090..15515
                     /locus_tag="PW101_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2512454.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15508..15957
                     /gene="nusB"
                     /locus_tag="PW101_000019"
     CDS             15508..15957
                     /gene="nusB"
                     /locus_tag="PW101_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDL2512455.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16054..16908
                     /locus_tag="PW101_000020"
     CDS             16054..16908
                     /locus_tag="PW101_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HDL2512456.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16909..18246
                     /locus_tag="PW101_000021"
     CDS             16909..18246
                     /locus_tag="PW101_000021"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDL2512457.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18251..18475
                     /locus_tag="PW101_000022"
     CDS             18251..18475
                     /locus_tag="PW101_000022"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDL2512458.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18477..19367
                     /locus_tag="PW101_000023"
     CDS             18477..19367
                     /locus_tag="PW101_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL2512459.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19376..20194
                     /locus_tag="PW101_000024"
     CDS             19376..20194
                     /locus_tag="PW101_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HDL2512460.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20301..20750
                     /locus_tag="PW101_000025"
     CDS             20301..20750
                     /locus_tag="PW101_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL2512461.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20768..22450
                     /gene="recN"
                     /locus_tag="PW101_000026"
     CDS             20768..22450
                     /gene="recN"
                     /locus_tag="PW101_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDL2512462.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22505..22621)
                     /locus_tag="PW101_000027"
     CDS             complement(22505..22621)
                     /locus_tag="PW101_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HDL2512463.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22694..23650)
                     /locus_tag="PW101_000028"
     CDS             complement(22694..23650)
                     /locus_tag="PW101_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2512464.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23821..24189
                     /locus_tag="PW101_000029"
     CDS             23821..24189
                     /locus_tag="PW101_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HDL2512465.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24359..24790
                     /gene="mraZ"
                     /locus_tag="PW101_000030"
     CDS             24359..24790
                     /gene="mraZ"
                     /locus_tag="PW101_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDL2512466.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24811..25770
                     /gene="rsmH"
                     /locus_tag="PW101_000031"
     CDS             24811..25770
                     /gene="rsmH"
                     /locus_tag="PW101_000031"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDL2512467.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25775..26176
                     /gene="ftsL"
                     /locus_tag="PW101_000032"
     CDS             25775..26176
                     /gene="ftsL"
                     /locus_tag="PW101_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDL2512468.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26177..28369
                     /locus_tag="PW101_000033"
     CDS             26177..28369
                     /locus_tag="PW101_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL2512469.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28395..29357
                     /locus_tag="PW101_000034"
     CDS             28395..29357
                     /locus_tag="PW101_000034"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDL2512470.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29371..30732
                     /locus_tag="PW101_000035"
     CDS             29371..30732
                     /locus_tag="PW101_000035"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDL2512471.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30758..31846
                     /gene="murG"
                     /locus_tag="PW101_000036"
     CDS             30758..31846
                     /gene="murG"
                     /locus_tag="PW101_000036"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDL2512472.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31861..33081
                     /locus_tag="PW101_000037"
     CDS             31861..33081
                     /locus_tag="PW101_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDL2512473.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33237..34562
                     /gene="ftsA"
                     /locus_tag="PW101_000038"
     CDS             33237..34562
                     /gene="ftsA"
                     /locus_tag="PW101_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDL2512474.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34585..35826
                     /gene="ftsZ"
                     /locus_tag="PW101_000039"
     CDS             34585..35826
                     /gene="ftsZ"
                     /locus_tag="PW101_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDL2512475.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35843..36520
                     /locus_tag="PW101_000040"
     CDS             35843..36520
                     /locus_tag="PW101_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDL2512476.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36534..37133
                     /locus_tag="PW101_000041"
     CDS             36534..37133
                     /locus_tag="PW101_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDL2512477.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37148..37435
                     /locus_tag="PW101_000042"
     CDS             37148..37435
                     /locus_tag="PW101_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDL2512478.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37484..38266
                     /locus_tag="PW101_000043"
     CDS             37484..38266
                     /locus_tag="PW101_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDL2512479.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38287..38994
                     /locus_tag="PW101_000044"
     CDS             38287..38994
                     /locus_tag="PW101_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDL2512480.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39256..42054
                     /gene="ileS"
                     /locus_tag="PW101_000045"
     CDS             39256..42054
                     /gene="ileS"
                     /locus_tag="PW101_000045"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDL2512481.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42103..43629)
                     /locus_tag="PW101_000046"
     CDS             complement(42103..43629)
                     /locus_tag="PW101_000046"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDL2512482.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43644..44291)
                     /locus_tag="PW101_000047"
     CDS             complement(43644..44291)
                     /locus_tag="PW101_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDL2512483.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44475..44804)
                     /locus_tag="PW101_000048"
     CDS             complement(44475..44804)
                     /locus_tag="PW101_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HDL2512484.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44981..45709
                     /gene="sfsA"
                     /locus_tag="PW101_000049"
     CDS             44981..45709
                     /gene="sfsA"
                     /locus_tag="PW101_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDL2512485.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45725..46738
                     /locus_tag="PW101_000050"
     CDS             45725..46738
                     /locus_tag="PW101_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2512486.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46738..48015
                     /locus_tag="PW101_000051"
     CDS             46738..48015
                     /locus_tag="PW101_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2512487.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48078..50780
                     /locus_tag="PW101_000052"
     CDS             48078..50780
                     /locus_tag="PW101_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2512488.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50932..51249
                     /locus_tag="PW101_000053"
     CDS             50932..51249
                     /locus_tag="PW101_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2512489.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51279..51599
                     /locus_tag="PW101_000054"
     CDS             51279..51599
                     /locus_tag="PW101_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2512490.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51707..53167
                     /locus_tag="PW101_000055"
     CDS             51707..53167
                     /locus_tag="PW101_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2512491.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53235..53456
                     /locus_tag="PW101_000056"
     CDS             53235..53456
                     /locus_tag="PW101_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512492.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53487..53669
                     /locus_tag="PW101_000057"
     CDS             53487..53669
                     /locus_tag="PW101_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HDL2512493.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53669..54082
                     /locus_tag="PW101_000058"
     CDS             53669..54082
                     /locus_tag="PW101_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDL2512494.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54205..55386
                     /locus_tag="PW101_000059"
     CDS             54205..55386
                     /locus_tag="PW101_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2512495.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55457..55621)
                     /locus_tag="PW101_000060"
     CDS             complement(55457..55621)
                     /locus_tag="PW101_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512496.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
BASE COUNT        18453 a         9430 c        12047 g        15899 t
ORIGIN      
        1 attgaataaa gaaagaagtt gaaaacgata cctgattaca aaaaaataac ttttataagt
       61 tctataacgt tgattatatt tttaattctt attcttctcg tcgtcttggt tccccgaatt
      121 tttggtttga ctccttatat tattgaggac aaagaaatga gtccatatta tgcacaagga
      181 aatctgatat atgtacgacc gaaaaataca aatgagattt tagtcggaga tattattaca
      241 tattatgaaa attcaggaaa aaaaatctcg acgcgtcgcg taatagccgt tgaaaacaac
      301 cacgaagatt tttatacaaa agctgataca aagacttata ttgagcctgg agtagtaaaa
      361 aagagaaata ttatcggtag tcccatcttt cagattccat atattggact agtcctttca
      421 aaaacggcat ttgcatggat tcaggggtta ttctttacga ttgctttttg cctaacagga
      481 atcacagcat ggaatacctt tgtagagttg aaaaagaagc ggagacgaac acagaaattt
      541 ttgtaagaat gttaaaaaca taaatgtatt ccagagatcc aacgactctt ctgaacatga
      601 aacacgagaa aacactcatg cagcttgtag atttagtcag ttgttcgtat aatgtgtgaa
      661 gaggaggagt agtatgcgca atgaaatgat gcatcgtcct gtagtcaaag aagaactatt
      721 ggattttatg cgtacccagc aaaaaaaatt accgggtgag ctaggcaaac tggaagaaga
      781 agctcatgta gcagaagtac cagttattcc ccatgaaaca gttgtatttt tgaaattttt
      841 acttggacag cttcagcctg aacgaatctt ggagatcgga gcagctatcg gtttttcatc
      901 aagtgtcatg gccacgacaa tcggagaaaa cgctcatgtt acgacaattg atcgtttcga
      961 tgtcatgatc gagaaagcta aaaaaaacta tgaacgtcta ggactgacag ataaagtgac
     1021 tttgcttgaa ggtcaagcag cagatatctt gccggagttg tctggtccgt atgactttat
     1081 ttttatggat agtgcaaaat caaaatatat tgaattttta cctgaatgct tacgtttgtt
     1141 aagaaaaggt ggcgttttga tggtagatga catcttccaa ggaggaacga tcttgctccc
     1201 agatgaagag attccccgtg gcaaacgagc tatccatcgt aaattaaatg aatttcttcg
     1261 tgttgtgatg gatcatcccg atttgacatc gacaatcttg ccattaggtg atggtgtgat
     1321 cttaatgaca aaggaatcag aaacaatcga gttgtgatac atgattatgg cagcctgtag
     1381 ataatcgatt gaatcgaggg ctcaattcga acttgatgta aaagtttcta tatttgtaag
     1441 tcaattaaag gatacaggaa atctaaataa acaggagtag catgaagtca atgtagctcc
     1501 tgttttcaat tgataacctt agaacagctg aaggagaatg tctcgtttta aaagattgat
     1561 ttagtagttg ggccaaaata attcattatt tgctaattaa ataactattt cgtattgaca
     1621 gaagcaattt aattatctat aattattgat gttccgctga ttcttgtcat agtagctcag
     1681 ctggatagag catccgcctt ctaagcggac ggtcgggggt tcgaatccct cctgtgacgc
     1741 taaaaaaccg ttagaaactg aggtttctaa cggtttttct ttgttttttg tgccaatatt
     1801 gtttgttttc aaaggcaaat aaaataaaaa aacatcctta tcgagtacaa ttataaggag
     1861 caaaaaaata aaactcatca gaaaagagga agaagatatg acgatactat ctagaatttt
     1921 agtgactcta gttgcaattg aattttttta tatcatgtat atcgaaacgg tgagaactga
     1981 ttctgacacg acaagtaggg tttttaaaat gagtaaagaa gagttaagca gaaaatctgt
     2041 tcaaacattg tttaagaacc aaggagtgta caatggacta ttaggagtgg gactacttta
     2101 tggagcttat ctttcttccg catctaaaga aatcacaagt atgctattaa tatctatttt
     2161 ttttgttgca ttatatggca gtttgactag cgatcgttgg attttactca aacaaggtgg
     2221 gctagctctg ttggcattgc tttctatttt cttataaata tagaaaaaat aagaatttat
     2281 ctaacaggaa actttgtgaa aaatattact ttatctcatg aaatatgata ttctaatgaa
     2341 tggtatttta gaaaatttca tgaaaaagga gttagatagg tgttcaaaaa ttatgtttcc
     2401 tcactaaaaa aagaattttc aggttatgat ctttcaagat ttcagaaaga tcttttggct
     2461 gggatcacag tagctgctgt agcattgcct ttagccttgg cttttggggt atcaagcgga
     2521 gctgatgcgg ccgcaggttt gattacggct atcattgccg gtatcgttat tggcggttta
     2581 tcaggcggtt tttatcaaat atcaggtccg acaggtgcga tggcagctat cttgatgtct
     2641 attgcggcaa caaagggaat gcaaggtgta ttgcttgcta cttttttagc gggtctattt
     2701 ttacttgcag caggactttt ccgattagga acactgactt cttttatccc agcacccgta
     2761 attacggggt ttacttcagg tattgctatc attattgcat taggtcagat cgataattta
     2821 tttggtgttc actcggaagg tacaaacgtt attgaaaaac taagtagtta tcaaacgata
     2881 ggatttccga taaacacagc ctctttgatc atgggagggc tagtcatttt agggatgttt
     2941 atctatccaa aaaaatgggc gaaacgaatg cctagttcgc tgctggctat cattttggca
     3001 acagcagtta tgctattgtt ccatttacca gtagcaactg tgggtaagat tccacaaaca
     3061 ttgatcagta gtaatcgttt gaatatggga gattttagct tttctgcctt gcaacaagtg
     3121 gcagttccag ctattagcat tgctttatta ggaatgattg aaagcttgct atgtggtgct
     3181 tcggctggcc gaatggcaaa taagccctta gatagcaatc aagaattaat ggcgcaagga
     3241 attggaaatc tgttgcttcc tttctttggc gggattcctg ctactgcggc catcgcccgt
     3301 acaagcgtgg ccatcaaatc tggtgcacaa actagattag ctggtatgat acatgcagtt
     3361 gtcttactgc tctccatgct tgtttttgcg ccagtgatgt cacagattcc aatgccagca
     3421 cttgcaggtg ttttgattgt gacggcttgg cgtatgaatg aatgggaaac gatcaaggag
     3481 ctatttacta ataaatattg gtcagcagtt ctattgttta tccttacgat gatttgtaca
     3541 gttatctttg atttaagtat tgctatcgtg atcggggtga tcggcggctg tgtatttttt
     3601 gttgtcaaaa gcgctgctat cacgatttca ttagaaacga tcgattggca aagaatgggg
     3661 ctgccagaaa cggaaaagct gaataactgg acagtagtgt atattagtgg tccactgttt
     3721 tttatgtctt cagaaaaact caaaaataaa ttggaagaac taaatcatca cgatggtgtc
     3781 attttctcca tgcgaggcgt accaagtatt gatttgacgg cacgtgcagt ttttgatgaa
     3841 tttcaagaaa aagcagagca aagaggacaa acgattatct atacttcctt gcagccagaa
     3901 gtagaaaaac aactttctca cttgtgggaa catacattca cgcatcaaca tcaaacagtt
     3961 gctcatgcgt tgacagcttt acatgcgaaa tatactgcaa ttagccatta aaacaatgaa
     4021 aaagaacaga caagactgta aactgaagat tttatggtct tgtctgttct ttttaatatt
     4081 gctgtaaaag tgaagctagt acttgccagt cttgatggat aacaggtaca agggagcgat
     4141 tgtagaaaat agctgctgga tgaaaaagtg gaagaattcg atagtttttt tctgaccatt
     4201 cgtaggcatc cttttgttct gttaattgta agacaggccc tgtatagagt tgaccatgat
     4261 ttttggtaac cgtatattct ttgccaagta aacgttgaag accaatgttt ccaagtgtcg
     4321 caattaattt tggctggacc ttcatcagtt cgtaatccaa tattggtgca tgcgcaaata
     4381 cttcggaacg agtaggtgtc cgatttggat aaacagtttc tgactgttgt gtccgtttat
     4441 tgatccgatg agtcacgcga tacgggcggc tccttacagc actggtgata tacacctctt
     4501 ctcgtgttaa gccagttgaa gaaagtgcct gcatcaattc ttttccagct tgaccgctaa
     4561 aaggaacatg cgtatcaatc tctgttttcc ctggggcttc tcccacgagc atcagtttag
     4621 gatgaagagg tccttgccca gcgataaatc cttctacagg acggccgttc attcttaaag
     4681 aagcttcttg tatcagttct tttgggtatt ccatttatgc tcctcctttt tcttttatta
     4741 taagaataaa agaaacacaa tgcgactatt ttatttggta gaactgtgag gatagaaaag
     4801 cagaagtatt gcttgaattt ttgttttact ttttgatttt aaatttagtt gactaacggt
     4861 tgctgttctg gtaagctagt actgttgtaa ttgtggactc cacagctaca accgctcaga
     4921 acaagtaata agcacagcat atgctgtcac ttcggatggc gagtctaagt atatcagaag
     4981 gaggtgcttt atagcatgta cgcaattatt aaaactggtg gtaaacaagt aaaagttgaa
     5041 gtgggacaag caatctatgt tgaaaaactt gacgttgaag caggagaaaa agttgttttt
     5101 gacgaagtta tcttagtagg cggagaatct acaaaagtag gcgctccaac tgttgcaggt
     5161 gcaactgttg aaggtactgt tgaaaaacac ggcaaacaaa agaaagtcgt tactttcaaa
     5221 tacaaaccta aaaaacattc acaccgtaaa caaggtcatc gtcaaccata tacaaaagtg
     5281 gttatcgacg cgatcaacgc ttaatagcgt ttgagaaaga aggcctatat atgatcaaag
     5341 gaacatttaa gcgaaatgac gcaggtcaga tcgtttcatt cactttgacc gggcatgctg
     5401 atgctggtcc ttatggaagt gatatcgttt gtgcgggtgt ttctgcgtta gcgatcagca
     5461 ctgtcaacgg gattgcctca ttagccggtt ttgagccgat tgttgaaatg aatgaagaag
     5521 aaggcggtta tctttatacc gaagtcactt caggaatgac acaagaacaa aacaatattg
     5581 ctgagattct attagaaaat cttttattag gattacaatc gatagaagct gaaaactcag
     5641 aatatatcca aattaaaaca attaatgata aataggaggt gcagaccatg ttgttatcta
     5701 tgaatctaca attatttgcc cacaaaaaag gtggcggttc tacatctaac ggccgtgact
     5761 cagaatcaaa acgtctaggt gctaaacgtg ctgatggaca aacagttaca ggtggatcaa
     5821 ttctttaccg tcaacgcggt acaaaaatct atccaggtgt aaacgtgggt atcggtggag
     5881 acgacacttt atttgcaaaa gttgatggcg tagtacgttt cgaacgtaaa ggccgtaaca
     5941 aaaaacaagt gtctgtttat ccagtagctc aagaagctta attgaatctt atgattaaag
     6001 aggttgtgac aagacttttg tcacagcctt ttttgtttat aaaaataaaa gttgtagtgg
     6061 aggctacgaa gtttactttg tctttttaca cttaatattt ttttgccaat ttggaatcag
     6121 gatgttttga taactatcgt tttcaattga tggaagctat attgtttgct atcagtgaaa
     6181 taattatatg gtaaacagtg ttctttttct tgtttccatt ataaaaatcg tatcgggatt
     6241 tttcgttctt ttaaattgaa catttctatc tatccgcgtg ttatcaacaa aacaaaatca
     6301 ggtaaatagt tgacgaaaca gaaataaata catagtatta tgttttgaag cctaagtaca
     6361 atagtctata gaaaaggagc gataccattg gcaaaacgca ttgaccgaat ttatatgtat
     6421 gtcaaagaaa aaaccgctca tttaacaaag gcagaatatg atcaaggagt aacaacacag
     6481 gaaattgctg aagtgttagg gattcagcga acaaatagca gtaaagatct gaatcagcta
     6541 gtgcgcgaag gaaaactgat aaagacagac ggacgaccag tacgttacat atatcaaaca
     6601 ctcgttacgc acgtaaagcc agttacaaaa catgtagtca gttacaaaga aacgccggtc
     6661 tctgaagaaa aaaatacact ccaaatagat actaaggata tctttgcaaa aatcattgga
     6721 gctaacggca gcatgaaaaa ttcagtggaa caggcaaaag ctgcgatttt gtatccgcca
     6781 agaggattaa attgtttgat tacaggacct accggatcag gaaagactta ttttgcacac
     6841 gcgatgtatc attttgcaaa agcgcatcat gtaatagatg aggaaacgga actgattgtt
     6901 ttcaattgtg ctgattatgc caataaccct gagcttttga tgagtcattt atttgggtac
     6961 gcaaaaggtg cttttacagg cgcagatgaa gaaaaaatgg ggattatcga tcaagcggat
     7021 ggcggtatgc tgtttttgga tgagattcac cggttgccgc ctgaaggtca agaaatgatc
     7081 ttttacttta tggatcatgg aacttacagt cgtcttgggg aaacaacgaa atcccatgaa
     7141 gcaaatgtac ggatcgtagg tgcgaccaca gaagacccag gttcttctct attagaaact
     7201 tttgttcggc gtatcccaat caatatcaaa cttccggctt ttgaaaaacg tcctgcaaat
     7261 gagaaagtcg atttagtaaa aatcatgatt gcccacgaag caaaccgcac gcagcgaaag
     7321 atttctttga cagaagatgt agcaaaagca ttgatcggca gtgtgaccta tggaaatata
     7381 ggacaattga agtcaaatat ccagttagta tgtgcacgag gatttctaaa tcatatgcaa
     7441 tcacccgaaa tctcgatcac aatcgatgat ttgacagaag ggattcgtag cggattgatt
     7501 caattagcaa gcaaccgcag tgcaatggca gagctttcta aaatattgga gccaaagata
     7561 actgtagcac caaatgatac gatcatgaaa atccaatcgg actcctatga gttaccttat
     7621 aatctatatg atatcatcgg agataaagca gctttactga agtcggatgg tttggatcaa
     7681 gaagcaatca atcatttcat ttctacagat atcaacatcc atctgaagtc tttctataaa
     7741 gaccatggtt tttcttttaa tgcagacagc aaattagcag aatttgtaga tccgaaaatc
     7801 atagaagtga ccaatcagat ttatgtaatg gtcaaaagat ctttaccata tgagtttcag
     7861 caaaacttta tttatgctat gagcttgcat atcagttctt ttttgaaaaa aatcaactta
     7921 ggagaagaac ggcatacgaa tgataacatc cgtgagatgg cttttgattt tccagatgaa
     7981 tatgctgttg cgaaagaagt gcgtagttat atcgaaacgt attttcaagt ggagattcca
     8041 gaaagtgaag actattactt gaccgttcta ctcgtctcgt tacgggcaaa tcaagcaagc
     8101 ggcagaatcg gtgtagtcgt tgcagctcac ggaaacagca cagcaagcag tatggtccaa
     8161 gttgtccaac agttgttaga tgcagagaac gtacgagcag tagacatgcc tttggatatg
     8221 gatccgaaaa cagcattgga ccgaattgaa cgcaatgtac aagaagtaga tgaaggaagc
     8281 ggggtcatac tcttagtaga tatggggtcc cttgcgtcgt ttaattcgca aattcaaaga
     8341 gacactggaa ttccagtacg tactgttgat atggtaacaa cttctctagt tttggaaact
     8401 gtacgaaagg tctcagtatt agggacagat ctagatatgt tgcatgaatc attgaaaaat
     8461 tttcgaggtt acgcagaaat atcttctgta gaatctgttc aggaagcaaa acctattcat
     8521 aagaaaaaag ctgttttggc tatctgcgca tctggcgaag gtacagcaca aagaatcaaa
     8581 gaattgatcg aacgagcggt aaacaagcgt caagaaacag aattagaagt tctagctttg
     8641 tcgattgtgg aagtgaaaga agaactgcca gttatccaaa aagaatatca gatcatagct
     8701 actactggga ttactgattt gaatatccaa gccccattta ttcctttaga acgttttatt
     8761 gatcaaaata tcgaagtcat tttggatcag ttgctgatgg aatcagaatt ggaggaaact
     8821 gaattcatct ccctagatga agagtcagct aaaaatacgt gtgttgagtt tatctctgat
     8881 aacttcatct ttattaatgg tagtaagtta atcgatccaa tgtggcaatt ttctacacaa
     8941 atctctcaga cgacgggaat cggcgatgaa gaatatggat tcaaaatcaa cctagtgatg
     9001 cacacagcag gaatgatcga acgtattatt cggaatgaac cattgactgt ggaagaaaac
     9061 gaattgacaa atacaacgaa tgatcctctt tatagccaat tggcagcctc agttgttctg
     9121 ttggaagatc agataaaagt aaaagtgccg atagaagaaa tgtattatct gctacgtcta
     9181 gtccataatc agttagataa aaaagaatac actgtgccat gaatacacaa aaacgataca
     9241 cttttatagt gtatcgtttt tgtgtattgg ttaagaatcc tttaaattca attagtgtat
     9301 cgtaaaaaag ttggcacgct ttttgcttat agtagtgtgt gctaggaggt ggttgttcac
     9361 acagaaatga taggggtttc tgtgaagtag tgaggaagaa ggagttttgc agatggatat
     9421 tcgattggca cgtatagatg atcgtttaat ccacgggcaa gtcgcaacag tttggtcgaa
     9481 gcaaacaggt gttgaacgta tcattgtgat cagcgatgta gtcgctcaag ataaattgag
     9541 aaagtttcta ctaaaagaag ctgcaccgcc ggggatcaca gcaaatgtaa taaccgtgca
     9601 gaaaatgatt caaatctatc atagtggatt attatcacga gcaaaagtga tgcttttatt
     9661 caccaatccc caagatgttg aagcggttgt ccgctgcggt gtacagttga agtcgttgaa
     9721 tatcggcggt atgagtttta ctgaaggcaa atcaatgatt accaattttg tttcagtaaa
     9781 tcaacaagat attgattcct ttcgttactt gaacagtcaa gaagtggagt tagagattcg
     9841 aaaagtacct gctgacagga aaatcctttt gatggaacta ctagcgaaaa tcaaaaaggt
     9901 gtagcaagaa tggttgttgg aaaaggtcaa ataattctgt tgtaatggtt agcaattatt
     9961 aatatttttg aatttgttac aacaaaagta aataggaggt ataaatatgg tagggattat
    10021 cctagcaagt catggacaat ttgctgaagg aatcttacaa tccggttcaa tgatctttgg
    10081 cgaacaagaa aacgtcaaag ccgtgatttt gaaaccaagt gaaggtccag atgacttgag
    10141 agccaaattg gaagaagcgg ttgcttcttt tgataatcaa gacgaagtgt tattcttagt
    10201 tgatttatgg ggcggaacac cgttcaatca atcaaacaca ttattcgaag agcacaaaga
    10261 taaatgggca atcgtcagcg gtttgaattt accaatgttg attgaagcct acgcatcacg
    10321 cttttcaatg gaatctgctc atgaaattgc agcacacatc attgaaacag caaaagacgg
    10381 tgtaaaagta aaaccggaag aattagaacc ggcagaagca ccgaaagcag cagttgaaga
    10441 cgcacaacct aaaggagcac ttccagaagg aactgtggtc ggagacggaa aaatcaaata
    10501 tgtattagca cgtgttgact cacgtctgtt acatggacaa gttgcgacag cttggacaaa
    10561 agcagtgcaa ccaaaccgta ttatcgttgt ttcagatgca gtatcaaagg atgatctgcg
    10621 taagagacta atcgaacaag ctgctcctcc aggtgtaaaa gcaaatgtta ttcctattag
    10681 caaaatgatc gaagtagcaa aagaccctcg ttttggaaat accaaagcat tgcttttatt
    10741 tgaaaatcct gaagacgtat tgacagctgt tgaaggtggc gtagacatca aagaattaaa
    10801 tgttgggtca atggcgcatt cggtcggtaa agtcgttgta agtaaagtat tgtctatggg
    10861 acaagaagac gttgaagcgt ttgagaagct tgaacaaaaa ggtgtgaaat tcgatgtacg
    10921 taaagtacct aatgattcac gcgataacat ggatgatatt ttgaaaaaag caaaagctga
    10981 attagcaaaa gcgtaataaa taatattaaa atttatagga ggcttatcat gtctattatt
    11041 tcaataattt tagtcgtatt agttgccttt ctagctggta tggaaggtat tcttgatgaa
    11101 tttcaatttc atcaaccact agtggcatgt acattgatcg gtttagttac cggtaacctc
    11161 gaagctggta tcgtacttgg cggtacgctt cagatgatcg ctttaggttg ggcaaatatc
    11221 ggtgccgctg tagcaccaga tgctgcatta gcatcagtag catcagcaat tattttagtt
    11281 ttaggtggac aaggtgttaa aggtgttcct tctgcgatcg cgattgccgt tcctttggct
    11341 gttgcagggt tgttcttaac aatgatcgta cgtacaatcg ctgttccaat cgttcatttg
    11401 atggatgcgg ctgctgaaga aggaaacatc cgtaaggttg aaatgtggca catcattgct
    11461 gtatgtttac aaggggttcg tatcgcggtc ccagcagcag cgttgctatt tatcccagca
    11521 caaactgtgc aatcattcct tgaatcaatg ccagcatggt taacagatgg tatggctatc
    11581 ggtgggggaa tggtcgtagc tgtaggttac gctttggtaa tcaacatgat ggctacaaaa
    11641 gaagtatggc cattcttcgt tattggtttc gttgtagctg caatttctca attaacattg
    11701 attgctctag gtgctttagg tgttgcatta gctcttattt acttgaacct ttctaaaatg
    11761 ggtggttcat caaataatgg aggcggaagc aattctggtg accctttagg cgacattttg
    11821 aatgactatt aatcttgaag gaggaaaata aaatggcaga agaaaaaatc aaattatcaa
    11881 aaaaagatcg tttagctgtt gcgtggcgct ctaccttcat tcaaggttca tggaactacg
    11941 aacgtatgca aaacggtggc tgggcatttt ctatgattcc agcaatcaaa aaattataca
    12001 aaacaaaaga agaacgttct gcagcattaa aacgtcactt ggaattcttt aatacacacc
    12061 catacattgc ttctccaatt ttaggggtta cattggctct ggaagaagaa cgtgcgaatg
    12121 gcgcgcctgt tgatgatgta gcgattcaag gggttaaagt aggtatgatg ggtcctttag
    12181 ctggtgttgg tgacccagtg ttctggttta cagtacgtcc aatgctaggt gcactaggtg
    12241 cttcattagc tatgggagga aatatccttg gaccaatcat cttcttctta ggttggaaca
    12301 ttattcgttg ggcattcatg tggtatacac aagaatttgg ctacaaagct ggttctaaaa
    12361 ttactgatga cctttctggc ggattgttac aagacgtaac aaaaggtgct tcaattttag
    12421 ggatgttcgt attagctgcc cttgtacaaa gatgggtatc aatcaaattc ttaccagttg
    12481 tatcaactgt aaaattagat aaaggtgctt acatcgaatg ggataaattg ccttcaggtg
    12541 gtgaaggaat caagagtgca tttgaacaag ttaataatgg cttagccctt tctcctgaaa
    12601 aggtaacaac attacaagac aacttagacc aattgattcc tggtttagct gcattaggat
    12661 taacattgtt atgtatgtgg ttgcttaaga aaaaggtttc tccaatcgtt atcatccttg
    12721 gtttattcgt agtcggtgtt gttggacacg tcatcggact tttataatcc ataaaaatat
    12781 agctcgggcg ttgtcccgag ctttttttat atagtatact gttcataaac aaatatctgg
    12841 agggagaaaa tggttcaatc aatcaataca aaagtagact tggtaattga tgctacttcg
    12901 tttacgggtc tgacagatta cgggcaaatc atgataggta ataatggttt tgagttttac
    12961 catgcgcgag atagacgcaa atatatccaa attccatggg aagaagtgga ttatgtacta
    13021 gcatcagtca tgctaaaggg aaaatggatt cctcgatatg ccattcaaac gaaaaaaaat
    13081 ggtacataca cattttcttc caaagaacct aaaaaagtgc ttcgtgcgat cagagaatat
    13141 gtagatgaag atagaatggt acaatcattg agtttctttg atgtagtgaa acgtggtatt
    13201 cgtagtatgt ttaaacgcaa gtaaaaaata aagaaaactt ctaagaggag gttttcttta
    13261 tttttttact ttgcagaaga aaatatgaaa tagcaaaacc aattatttgt cccctttaaa
    13321 tcccatatgc taaaattgat tagcaaagaa ggaggttata ttatgagttg gttatggaca
    13381 ttgattgttg gagccgtgat cggagcaatt gcaggtgcga tcaccagtaa aggaaattcg
    13441 atgggctgtg taacgaatat cattgctgga ctagtcggtt caagtattgg acaagcgatt
    13501 ttcggcgaat ggggaccaaa agctgcaggt atggcactta ttccatctat tttaggcgca
    13561 gtgattttgg tagcagttgt ctcatttttc tttggaaaac gatcataagt ttttacaaaa
    13621 tagtaaacta aaaggagttg cagcgagact gtgactcctt tttggcttta tttatttaaa
    13681 ataaaaagaa gttttccgga aatttttatt taactgtcgt gtacgaagaa aagtttgcta
    13741 taatggaaaa aagcgtgcaa agaaagaggg agtttcatta tgatgctacg tgtagaaaaa
    13801 ttgcgcaaaa aaatgcaaga agaaaatttg gattcatttt tggttaccag tccgtacaat
    13861 cttcgttact taacgaactt tacaggtacg acaggattag ctgttattac tttggaaaaa
    13921 gcatttttta ttactgattt tcgttatacc gagcaggcgg cagcgcaggc tcaagggttt
    13981 gaaattatta aaaatgtcgg acctattttt gatgaggtag caaacttagt acgcaaagaa
    14041 gaactgcact cattagcttt tgaggaaaca acagtctcat ttttagaata ttcggtttta
    14101 gaagaaatca tcgatgcgga attagtacct gtttcacaga tgatcgaaga actcagagaa
    14161 gtgaaagatg aagaagaaat cgctattatc gaaaaagcat gtcatattgc ggatatggca
    14221 tatgatcaca tcctgaaaat gatccaaccg ggcatgactg agattgaagt cgcaaatcaa
    14281 ctagactttt atatgcgttc tcttggcgct tcaggtgttt cttttgatac gatcgttgct
    14341 agcggattgc gttcggctat gccgcatggt gtagcaagca aaaaaatcat cgaacaaggt
    14401 gacttgatca cattggactt tggttgctat tacgaagggt acgtttccga tatgacacgt
    14461 acattcgcta ttggtgatcc tggggaaaaa ttaaaagaaa tttatcaaat agtcttagat
    14521 gctcaattag cagttattga tgctgcaaaa ccaggaatga ctggtgtcca attagatgca
    14581 gttgctagag attatatttc aaaacatggt tacggggaag catttggaca ttctacagga
    14641 catgggatcg gtttagagat ccacgaagga ccaaacgttt cttctcgtgc agaaaagcaa
    14701 tttgtaccag gaaatgtgat cactgatgaa ccagggatct acttaccagg aatcggtgga
    14761 gtacgtattg aggatgactt gttgatcaca gaagatggaa atcgtgtgtt gactcgttca
    14821 ccaaaagaat tgatcatctt atagtttcat caatagaaga agtaagcgta aaaatcagtg
    14881 ttcacagact ttgttataga aaatcactcg atttaatgat acactataga agaactttta
    14941 agctgtaaga gataataaag ttgggtgttt tctccaattc atagcagttt gcagctgatc
    15001 ggcggcagtt ttcttaccac gtgatcacta tgccgcatca tagagaattc ggatgtatct
    15061 cgtgcttagg caaagggagg aataaataaa tggcagatga aaaaaatttg attttaaacg
    15121 caaaccaaga tttaggagag atcgtgatcg ctcctgaagt catcgaagta attatcggga
    15181 ttgcagcttc taaagtagaa ggcgtatacg gaatgcgcgg aacttttgcc agcaatgtca
    15241 ctgaattatt aggacgtgca gctcacggaa aaggtgttta cttagcaaac gaggaagatg
    15301 gcttgaaagt agatctttac tgttatctag aatatggtgt ttcagttcct aaagtagcaa
    15361 tggaaatgca ggaacgggtc agccagcaag tccttttcat gacggatatc gatttatctg
    15421 aagtcaatat ccatgtagta gctgttgttc ctgaaaaatt agaacaacca acaatcgaag
    15481 aactttttga agacgaagag gaagagaatg aataaagaat tatcaagaca cgagattcgg
    15541 gaaatggcat tgcaggcttt atttccatta gacttcaatg cagacctaac aaaagaagat
    15601 gcgattttca acgcaatcga attagaccac cgcgacatga tcaacgaaga tgaatcagag
    15661 tttgttcctg tttatcttga tacgctagtt ggcggtgtct gtgcaaaaaa agatgaattg
    15721 gataaagtga ttgaaaaaca tttaaaaaac aattggcaca tcaatcggat ctcaaaaatg
    15781 gatttggtga tcttgcgtat cgcgattttt gaaatgactt atgtgacaga tgtaccagcg
    15841 gctgttgctt taaatgaagc aatcgagttg gcaaaaacat ttagtgatga ccgttcacgt
    15901 aaattcgtca acggtgtttt atccaatgta ttgaaagaat tagaagctgg ggcgtaagtc
    15961 ctagcttttt tctatgaatc atcataaacc tgttataaat cgggaaaatt ctatggtaga
    16021 atgaaaacac aaaaaatatc tgggagtggt tagatggcag aattgattaa cggaaaagaa
    16081 ttagctgaaa aaatgcaggc agagattgct gaaaaaatcc aaaaattgaa agataatggt
    16141 atccatcccg gattagtcgt tttattggta ggggaaaatc cagccagcca aatctatgta
    16201 agaaataaag aacgtgtagc aaaagaaatc ggaatccatt cattggtaga gcgttatcct
    16261 gagacgattt ctgaagacga actacttgca gaaattgaaa aatacaatca agatcctagt
    16321 tttcatggta tattggtcca attaccattg ccaaagcata tagatgaaga aaagatcctg
    16381 ttagctatcc gccctgaaaa agacgtagac ggttttcatc cgatgaattt aggcagactc
    16441 cttgcgggta aaccggacaa gattccttgt actccatacg gcatcatgaa aatgtttgaa
    16501 gcttattcta tcgatccggc gggtaaacga gcagtagtta tcggacgaag caatatcgtc
    16561 ggtaaaccga tggctcagct actcttgatg gcagatgcaa ctgttactgt tgctcattct
    16621 aaaaccgaaa atctggctga actggcacaa gaagcagata ttttagtcgt agcaatagga
    16681 cgtggacatt ttgtcacaaa agaattcgta aaaccaggtg ctgtagtgat agatgtaggg
    16741 atgaatcgtg atgctaacgg taagctgatc ggtgatgtga aatttgatga agtggagcct
    16801 attgcaggct ggatcactcc tgtgccaaag ggtgtagggc caatgactat cacgatgctg
    16861 atgtatcaaa ccgtaaaatc tgccgaagct tttggagcag gtgaatagat ggcacaagaa
    16921 tatttaactg tcactacatt aacgaagtat ttgaaaagaa aatttgatgc cgatccttat
    16981 ttagaacgcg tatatttaac aggcgaaata tcgaatttcc gtttacgtgc caatgcccat
    17041 caatatttca gcttaaaaga tgaccatgca aaaatatctg ctattatgtt caaaggagca
    17101 ttccaaaaat tacgattcca gccaaaagaa ggaatgaaag tccttgtcgt tgggcgtatt
    17161 tcattatatg aagcgggagg ttcttatcaa atctatgtag agcacatgga gcctgatgga
    17221 ataggtgctt tatatcaaga attggaagaa cgaaaagaaa agctgatgaa ggaaggcctt
    17281 tttcaagggc ctaaaaaaat gctgccacgt ttcccaaagc gaatcgcggt ccttaccagt
    17341 ccaagcggag cggttatccg cgatatcatc acgacggtga aaagaagata tccaattgtc
    17401 caattggttt tgttcccaac agttgtacaa ggaaaccaag cagcggacaa tgttgtcagg
    17461 aatatccaga gagtagaaga gatgggcaat ttcgatacaa tgatcatcgg tcgtgggggt
    17521 ggttcgattg aggatttatg gccgtttaat gaagagcggg ttgctcgggc gatttatcaa
    17581 gctaatacgc cggtgatttc atctgtagga catgaaacag ataccacgat cgctgattta
    17641 gtagcagatg ttcgggctgc cactccaaca gcagctgcag aattagctgt tcctgttttg
    17701 tcagaagagt tgatgcgcat tcaagaacgg caagctagat tgcagcaggc gtttgtacgc
    17761 cagatccagc gcaaacaaga acggttcgag cgtgctcgac gttcctatgt atttcggcag
    17821 cctgaaagat tgtatgaagg tcagaccatc aaattagacc agttgaaaca gcgattatat
    17881 caagctacac agcaaattta tcatgcgaaa gaaaaacaaa cgatcgcatt aagccaccaa
    17941 ttggaacaag tggctccgct ttatcgagtt aaagcagctc gccaagaaac tgcttatcta
    18001 gaaaaacgct tagaagagaa aatccaacaa tatatgaaac aacagcagca gcgttttgct
    18061 caagctgttc aatctcttga tttgttgagt ccattgaaaa tcatgggaag aggctacagc
    18121 tatacgacgt taaatgatca agtagtaaaa agtgtcaccg agattcatca aggggatttg
    18181 ctgacgatcc attatcaaga tggaatcgtt aaaggacaag tacaagagat agacaaagga
    18241 gaataaaaat atggaaaatc caacttttga agagtcactg caagaattag aaaaaattgt
    18301 catgcagtta gaacaaggag acgttccatt agaatcagct ctagattcgt ttaaacgagg
    18361 aatggaatta agcaaacatt gccaagatac attagctaaa gcagaagaga ccttgacgaa
    18421 aatgatgaca gaatcaaatg aagaagttga ttttgacgga aacgaggaaa gctgagatga
    18481 gattaactga cttttcagct attcatctac ctgctgtaga aaaagaaatc ttgtcttttt
    18541 tagatgagca tacaacggat agaggactct atgatgcgat gagttattca gtgaaagcag
    18601 gcgggaaaag aatacgtccc ttattacttt taacggccgt tgcttccttt gatgagccta
    18661 ttgatgttcc cgtctatcaa gtagcagctg ctttagaaat ggtgcatacg tattccttga
    18721 tccacgatga tttgcctgcg atggacaatg atgatcttcg tagaggaaaa cctaccaatc
    18781 ataaagtatt cggcgaagca ttagctatat tggccggaga tggtttgctt acaggagcat
    18841 tccagctaat cagtatggca cacttaggta attctcctaa gcttctgctg cttcagcaat
    18901 tagctgtttg tgcgggatca caaggtatgg ttgctgggca agctgcagat atcgagggcg
    18961 aatcgaaaaa gctttcttta gaagaattag catttattca tgaaagaaag acagggcatt
    19021 tgattcgtta tgccttgctg gctggtggca tattagcgaa gcaacctgaa gaaatcttat
    19081 tgctacttca acgattagcc gaacatttag gtttagcttt tcagataaga gatgatctac
    19141 tcgatgtgat tggtacaacg aaaacattag ggaaaacagc tggtaaagat gaacgtatgg
    19201 aaaaaaatac ttacccgcgt ttattaggcc tagaaaagac acgtgaggcg ttggagattg
    19261 agctgttatc cgccaacaag atcatagaca aattagaaga ggacgttttt tcttttgatg
    19321 gcgggctgat gcgccagatg atcaagcaat ttgatgtgga gaaatgaggg aaagaatgga
    19381 aaaagaacgc gtagatgtat tagcggtcaa acaaggatta tttgaaacaa gagaacaagc
    19441 caaacgttca gtgatggctg gtttgattta caacgaaaaa aacgagcggt ttgataaacc
    19501 gggagaaaaa attccagtga gtagtgaatt aagagtcaaa ggtaaaaaac ttccttatgt
    19561 ttctcgaggg ggattaaaat tagaaaaagc actcaaagca tttgatctat ctgttgaagg
    19621 aaaaacattg ttagatattg gtgcatccac tggcggattt actgatgcag ctttgcaaaa
    19681 tggcgcaaaa atgagttatg ctttagacgt tggctacaat cagctagctt ggaaaataag
    19741 acaagatcct agagtcgtcg taatggaaag agtgaatttt cgttatgcaa aacccgaaga
    19801 ctttacctta ggaattccag aagttgctgt tatcgatgtt tcttttattt cactaaaact
    19861 gatgctgcct ccattacatg ctattttaaa agaagaggga gaagtgattg ctttaatcaa
    19921 accccaattc gaagcaggca gagaagctgt agggaaaaac ggaattgttc gtgaccctca
    19981 gacccatcaa aaagttttag aagacatttt gtcatttgcg gcacaaggtg gatatgatgt
    20041 gctggaatta agttattctc cgatcacggg gggagaagga aatatcgagt tcttggctca
    20101 cttgaggaaa gtacccgaaa gcggaacaat caattcagct attaatatgg cagaagtggt
    20161 ttcaaatgca cacgaacagt ttgaccataa ataaattgta actgtgaaaa tcaagtgaga
    20221 agtatgcgga ttcttcttta ttataccaag gaaataagct atcattgatt aagaagaata
    20281 tgctaaggag cgaagacgta atgagaaaaa aagatcggca tcgtttgatt actcgattgt
    20341 tgagtgagca agatatccga aagcaggaag aatttgttga aatcctgaaa agcaaaggaa
    20401 tatctgttac tcaagctacg atttccagag atataaagga actgaaactg atcaaggtac
    20461 cggcttcaga tggcggctac cgttatagca taccagctga aacaaacgaa gatgtaaata
    20521 ctaaattgga aaaactatta aaagacgctt tcgtggcagt tgaccaaatg gaaaaatttg
    20581 ttattttgaa aacattgccg ggaaatgctt cggcagcagc aaatttgatt gataaacgtt
    20641 acaagaaaga attattttcg attatcaatg atgacgataa tgttttgatg attacacgaa
    20701 ctgaagacca ggcagtttta ttgaagaaag acttcctgca ttacctttaa gttgaggagc
    20761 gaatgtgatg ctacaagaaa tcagtattac taactttgcg attattccgg aattgcggct
    20821 ttcttttcac gaaggaatga ccgcgctgac cggagagact ggtgcgggta aatccattat
    20881 tatcgatgct ttaggtctgt tagcaggagg aagaggttca agcgattata tacgtcaagg
    20941 agcagaaaaa tgtgtgcttg aagggttgtt tgaactacca aaacaagaag gattctctga
    21001 actgatggtt gaattaggga tcgaaacaga tgaggacaat ctgattgttc gacgggatat
    21061 gtccttaact ggaaaaaatg tttgtcgagt gaatggtcat attatcacat tagctaatct
    21121 gagaagaatc ggcagttatt tagtagatat ccaaggacaa aacgaacatc aggaactgct
    21181 ccagcctgaa tcacatcttg ctttattgga tcgttttgga gatgtagctt tccaacagaa
    21241 gaaaaagagt tatcagcaag aatatatctc ttatcgtgag ctggaaaaaa gggtacgaaa
    21301 agtgcagcaa aatgaaaaat cctatgttca gagaatagat atgctccatt tccagcaaga
    21361 agaaatagct gctgctgatt tgcaagtcgg agaagaagag aaattaaaag aagaaagaga
    21421 aaaacttagc aattatcaaa aaattgctga tggattagct gcaggttacg gagcactgac
    21481 gaataatgaa caaaacagtg tagatggtgt tggcttggcc gtttcagaac tgcaagggat
    21541 cgcccatcta gatgtggagt atgaagcaat ttatgaaaat atccaaagtg cttactattt
    21601 gctgcaagat gcaatcggcg atatgagtcg tcagattgat ttattggagt tagatgagag
    21661 tcgtttagaa gaagttacgc aacgtttaga attaatccga cagttgaaac gtaaatacgg
    21721 cgaatccatt gagtcaatat tggcttacta tgacgaaatc accgaagagc tagcttcttc
    21781 tgatttttca gaaggtcaat tggacaaaat gaaagaggaa ctagagcaaa aagagcttct
    21841 acttcagcaa caagccgcag atcttcatga agcgcgtaaa aaaatcgcaa aagaattgga
    21901 aaaatccatt ctgcatgaat tgaaaagtct gtatatggaa aacactgagt ttgaggtacg
    21961 tttcttaaaa gaagaaaata gacaattgaa tcatgatgga tttgaccaaa tcgaatttta
    22021 cattacgacc aatccaggag agccactgaa gcctttggtg aaagtcgctt ctggtggcga
    22081 actgtctcgg atgttgcttg ctttgaaaac tattttttct tctgagcaag gtgtgacaag
    22141 tattattttt gatgaagtgg atacaggtgt gagcggaaga gttgcacagg ccatcgcaga
    22201 taaaatttct caaatatcga aatactctca agttttgtgc atcactcatc tgcctcaagt
    22261 tgctgcagtc gccgactatc aatattatat tgtgaaagca gtgattgatg gacgtacgca
    22321 aacttctgtt tcagagttga aaacaaaaga aagagaagaa gaaattgcac gtatgctggc
    22381 tggaagtgag attacaaaac tcacactcga acatgcaaaa gaactattga aactcgcaaa
    22441 aaggacctga gccacctcgc agtggttcag gtccttttca gcagctgata gctatatttt
    22501 atttttacat gacactcatt acagcagtca atacaacaga tccaatcgtt aaaatcagca
    22561 ttgtccaaat gacaaatttt gttactttag caaaagtact tgtttgtttt ttttcacgca
    22621 ttgttttcaa cctcgtcatt aattagttga ttcaagtgta caatcttttt tccatttcca
    22681 caagagggct tttttataaa aacttccctt tttttaaata ataggtagtt acgatacaaa
    22741 taaataatgt taatagaact agccatataa aggcatcatc cctttcagca ataggtaact
    22801 taatgttcat tccatagata ctgccgacga ttgtcggtat agttaaaata atcgttaaag
    22861 aagttaaaat tttcataata ttattcagat tattcgatac gattgctgaa aaagtctgat
    22921 tcatttgttc tactagtttt aattgaatgc gagaagtagt cattgcctgc tctgtttcca
    22981 ccaatacatc acgtaaatga tttttcaaag catgattttc ttggaaaagt ttattttgcg
    23041 ctagttcttt caatgcatct aaattagcag aagtcgcttc tctgaaataa acgaggcttt
    23101 tttggatatc catgatttga taaagttgtt tgttttcagt ggatacttga agtttggctt
    23161 ccaaagtatc gcattgagat tgaagcgtag ctaaataact gttgtaagag agtgtgacct
    23221 gccaaatgaa ttgaaagaag atattgagat taggcgaaat atcacttttt ggaaaggtct
    23281 ggctaaagac tttctttaaa aacagtggtt catgattcgt aattgtgata agcttctttt
    23341 tgggcgtcaa aataatagag agcgggtatg tatccacttg aaaataaccg ctaggacttg
    23401 ttttagcaaa aggatattgt aacaataata acgctggctt tgtcaacttt gattgattga
    23461 attcttctgt acgggaattt tctgcatcgt ccaatatgga agtgatgtaa tctgacggaa
    23521 tttcaaattg atgtgtcagc tgatgaatct cttcttgatt aggacgttct acacaatacc
    23581 agatcgtttg gtcagtttcc tttccggtat tacgcagttt ctcattatct aatgtaaaat
    23641 atttgatcat gattttcctc ctgttgcctg tttcttttat tataacggat agggaattgg
    23701 aatacatgga atgtgtttgt aagatataga atataactgc ataaatttaa ataaaatgaa
    23761 acagttgtgt aaagtatata cagtatactt gaagcaacga taaaaaagga gtttacgttc
    23821 atgggcacat tcacaccact tatcttgttt tggtatattt acccggtaat cgttttgttt
    23881 ggttgccaat ttcttgtaaa attattttcg ttaaatcgtc gttttaaaat aaaagcccct
    23941 gatttggcga ttcctttttt gtggggcggt atgcatgcat tgtctagaaa tacagggaca
    24001 atttctatct taccgttttt ctttatttct gttttgctaa tgggaatttg tattgctgtt
    24061 ttccaagcat attattatga agaaattatt tatccgcgtt atttcaagat gacttggcgt
    24121 ttggtttttc tgttgacaat cgtcttgtac gctgtcttga tcattgtgaa catactttct
    24181 tacctatgaa aaaaatagaa aagctgtaaa aagtctttta aaaggctttt tgcagcttga
    24241 tttttaaaaa aatgtttatg aataaaaaaa gttgttcccg gatttcccag aaaaggtggt
    24301 agaaagtggg ggattgtggt agactaattt tagttagtgg aggaatgggg ggctttcaat
    24361 gttgatgggt gaattccaac ataatattga tgcaaaagga cgtttaatcg tgccctcaaa
    24421 acttcgtgaa gaacttggcg aaaagtttgt tctgacacgc ggattggatg gctgtctttt
    24481 cggctatccg atgagcgaat gggaaaatct ggaagctaaa ctgaacgaaa tgccgttggc
    24541 taaaaaagac gcacgtactt ttgtgcgatt cttctattcg gctgccactg aatgcgaact
    24601 tgataaacaa ggacgaatca atatacccag cacgttaaga aattatgcag ctttgacaaa
    24661 aggttgcgta gtcatcggtg tatctaaccg aattgaaatt tgggatgaga cacgatggca
    24721 agatttttct gcagcagcag aagagaactt tgacgaaatt gctgaaagta tgattgattt
    24781 cggactataa gaataggaag aggctgacta atgactgaaa catttcaaca ctatacagtc
    24841 atgttgaaag aaacggtaga tggattgaac atcaaaccgg atggcgttta tgtagactgt
    24901 acactcggcg gtgccggaca tagtgaatat ttattaagcc aactaagttc taaagggcat
    24961 ctgtatgcgt ttgaccaaga tcaaaaagca atcgatcacg ccaagctgcg tttagcttct
    25021 tatgtcgaac aaggtatggt cacatttatc aaagcgaact tcagagaatt agaagaacgc
    25081 ttgagtgagc atgtagataa ggtagacggc attttgtatg atctaggggt ttcctctcct
    25141 cagctagatg aagcagaacg tggatttagt tatcatcaag atgcaccgtt ggatatgcgc
    25201 atggatcagt cagctccttt atccgcttat cacgtaatta atgaatattc ctaccatgaa
    25261 ttagtaaaga ttttcttccg ctatggcgag gagaaatttt ccaaacaaat tgctcgtgaa
    25321 atcgaacgag tacgaaaaac aaaaccaatc caaacaactg gtgagcttgt cgaaatcatt
    25381 aaacaagtca tcccagctcc cgcaagaaga aaaggcgggc atcctgcaaa aagaattttt
    25441 caagctgtgc ggattgcagt aaatgacgaa ctcggtgccg aagaggcctc tttagaacag
    25501 gcaatccggt tattgaaagt caatggaaga atcagcgtaa tcacattcca ttcattggaa
    25561 gacagaattg tcaaaagtat gtttaaagaa tatagtacgg tacaagatct accaccggga
    25621 cttcctgtca tacctgacga attccaacca gaactgaaag tcattactcg taaaccaatc
    25681 cttccttcag aggaagagct aacagaaaac aatcgatcac gcagtgcaaa gttacgtatt
    25741 gcagaaaaaa taagaatgaa agaggagtag aatgatggca gaattaaaga aaatgcaaga
    25801 ttacgaatat gatttacctg aattggtgga tcagccggaa caacaaatgc ctgattcttc
    25861 ttcgaatcaa gaaatcattc ttccacaatc accggcaaaa cgattaaagc agatttctgc
    25921 tttggaaaaa attgctgtgg caagcgtggc gattgctatt gttgctttat gtgttttaac
    25981 agttatgctg cgcacaaata tcagcggagc tgaaaaagaa atcacgtcaa tacaaacaga
    26041 aatcaatgat aaaaagcaag aaaaaacgag tttagaacaa gaaaaaaacg aactttctcg
    26101 tactgaacgg attaaacaaa tcgcagaaga aaaaggattg tctattaatg acgacaattt
    26161 aaggaaagtg aagtaaatga gtcttaaaaa taaaattcgg cggtttatgg aaaagaaaaa
    26221 tctaaatccg atgaacaatc gcaaaaaagt aggtatcatt ttatttgcta cgagtattgg
    26281 attgttcttt ttatttgccg tccgtttctc gtatatcgtt attggaggtc atgttgctgg
    26341 tacctcatta gctgaaaaaa caaaacagtt gtaccaagga agtgaagtag taaaagcaaa
    26401 aagaggaacg atttatgatc gtaacggtgt ggcgctcgca gaagatgcct cctcttattc
    26461 aattaaagcg atactctcaa aaacatatac ctctggggat aaaaaactgt atgttgaaga
    26521 aaagaatttt gataaaatag cagaaatcct gcataagaat ctttctatag acaaaaaaga
    26581 tgcactgaat attttagagg acggcgcaaa aaaagagctg tatcaagttg aatttggtag
    26641 ctatgggaaa aatatctccc aagaaaccaa gcaaaacatc gaagcggaca tgaaaaaaga
    26701 aggtgtcgct ggtctttatt ttgtcgacca tcaggctcgg atgtatccaa atggcgtctt
    26761 ttcttctcat tttattggat acgcagttcc agacaaagat gagaatggct tagttggtaa
    26821 actaggtttg gagtctgctt acaatgatat cttgagcggt aaagatggta agattattta
    26881 tcaaaaagat aattttcaaa atcctctgcc aggtacagtg gcagaagaag aaaaagctgt
    26941 cgacggacaa gatatctaca cgactttaga cagcagacta caaagttatt tagaaacact
    27001 gatggatcaa gtaaatgaag aatatcagcc agaagaattg acagcagtcc tgatgaaagc
    27061 aaaaaccgga gaaatcttgg caatgggaca acgacctact ttcaatcctg aaacaatgga
    27121 aggattgact ggaaaagatg ctatctggcg taatttcttg gtacaagata gttatgaacc
    27181 aggatcaacc atgaaagttt tcactactgc agctgctatc gaagaaggag aattcaacga
    27241 gaatgaaacc ttccaatctg gaaaaatcca agtagaagat gctacgatca atgaccatga
    27301 ctttggtgaa aaaggtgtgc tgaccatgcg tcaagctctt tcatggtcaa gtaacgtcgg
    27361 gatggtcata ctggaacagc gattaggcgg acgatggtat aactatttac aaaaactggg
    27421 atttggacaa agtactcatt ctggattaga tgatgaagta aacggtgctt tacctacatt
    27481 aaatattgtc gaccgagcaa tgagtgctta tggacaagcg gtaggtgtga cgaatttcca
    27541 aatgatgaaa gctttcactt ctattgcgaa taacggtaca atgattcaac ctcgatatat
    27601 cagcaaagtg gtggatcccc aaactggaga agagcgaaca actcaaacag aagttctagg
    27661 gcaacctttt tcaaaagaaa caacagaaaa agtccgtgaa tatatgagag atgttgtcga
    27721 aagcgaaaac tacggtagtg cttatggtgt atatagtgta cctggataca atgtatcggc
    27781 aaaaaacgga acagcccaaa tcgcttctga tacaggggga tatcaaacag gagatacagc
    27841 ctacctgtat tcgattgtgg agatggttcc ttctgaagat ccagactatg tcttgtatct
    27901 aacaatgaaa caccctaaga catatgatcg aatggcattg gctaaaatcg caaatccatt
    27961 gatgaaacgg gcaatggatt ttaaagaaac tgaagaagat tcagatacag aaacaaaaac
    28021 agaaaaagta tccgtagccg attatcgtaa tcttgaagcg gacgttgcag cagctgatgc
    28081 tcaaaaaagt ggactgcaac ctgtagtgat cggtaatggt aaaaaagtac aaaaacaatc
    28141 tactgccaac ggtgatcagt tgatctctgg agaaaaactg atcctatata ctggtggtga
    28201 taagctgatg cctgacgtaa caggatggtc aaaagcagat atcatgaagc tggggaaaat
    28261 cctcggtatt gaagttagtt ttgatggaga tggttattgt gtcaaacaag aacttgctcc
    28321 atatgaaaaa atcactgaag acaaacttag ttttacttta gaagaataag aagagaaata
    28381 ggagagaaaa gagcatggaa tggacacaag cattgattcc cattgtcagc agttgtgcaa
    28441 tgacaattgc cgcaatgccg ctatttattg gttattttca aatgaaaaag caaggtcagg
    28501 cgattcgtga ggagggacca aagtggcaca atagcaaagc cggaactcct acaatgggcg
    28561 gcttagtgtt tctgatcggc tctattttga caggaatttg ggttggagct tggcaaaaac
    28621 agctaactcc tactcttttc atcttgcttt ttgtattagc gttatacgga gtaatcggct
    28681 ttcttgatga ttttataaaa atattcaaga aaagaaacat gggactgaac tcgaagcaga
    28741 aattgattgg acagattcta gggggaatca tcttttatct tgtttaccgt tctgaaggtt
    28801 atccaggtgt attgaatttc ttcggactag atctaccttt aggaatcatt tatggtattt
    28861 tcgctctctt ctggcttgtt ggattttcaa atgcagtaaa tctgacagat ggcatcgatg
    28921 gattagttgc tggcttagga agtatctctt ttgcagctta cggtctaatt gcttggcatc
    28981 agcatcagta tgatgtatta atcatttgct taagtgtttt aggaggttta ctgggtttct
    29041 tcgtctacaa cagaaaacct gccaagattt ttatgggaga tgtcggttca ttagcgttgg
    29101 gcggactttt agcggcaatt tctataatgc taaaccaaga atggacattg cttttgattg
    29161 gtttgatcta tgttatggaa acagcaagtg tgatgcttca agttacttcc tttaagctta
    29221 caggaaaacg aatttttaaa atgtctccaa ttcatcatca ctttgaaatg tgcggatggt
    29281 cggagtggaa gattgataca gttttttggt tgatcagcat tgtgacttcg ttgatcacat
    29341 tatggatcgt ttggtaggag atgacgggaa atgaaaaaaa tatcaacata tgaaaataag
    29401 aaagtgcttg tcttaggact cgcaaaaagc ggagtaagtg cggccaaatt attacatgaa
    29461 ttgggcgcac ttgtcaccgt taatgatgga aaaccttttg acgaaaatcc ggaagcacaa
    29521 gagctgcttt ctttagggat aaaagtaata acaggtagtc atccaatcga attgttggat
    29581 gaagaatttt cattgatggt caaaaatcca gggattccct acagtcatcc tttagtagca
    29641 aaagcccaag aaatgggtat tccggtaatt acggaagttg agctagctta cgaagtagcg
    29701 gaatgcccaa ttattgggat tacaggaacg aacggaaaaa caacgacaac aacgatgaca
    29761 ggactactac tgaatgcagg agcagatcaa ggaattgcac gtttagcggg taatattggg
    29821 tatccagcaa gtggtgtagc gcaagaagca aaatcagaag ataaaattgt gatggaattg
    29881 tctagcttcc aactcatggg aatcacagat ttccgccctc atatagcggt gattaccaat
    29941 atctatgaag cacatattga ttatcatggt acaagaaaag aatatgtcaa agctaaatgg
    30001 aaccttcaaa aaaacatgac agaaaaagat tatctgatcc ttaactggaa tcagtcagaa
    30061 ctacaagaat tagcgcaaag aacaaaagca cgcgtactgc ctttctcgac aaaagaagtg
    30121 ttggaagacg gtgtgtatgc tgacgattac tcaatatact ataaaaaaga aaaaataatg
    30181 gaaatatctg aacttggcgt accaggtaag cataatgtag aaaatgcact agctgctatt
    30241 tctgtagcaa aattgtatgg tatatcaaat gaagctatac gagaaacgtt gcattttttc
    30301 catggcgtac cacatcgtac gcaatatgta ggagaaatcc aaggcagaaa attttataat
    30361 gattcaaaag caaccaatat tttagcgact aaaatggctc taagtggatt tgaaacgtct
    30421 aaggtcgtgt tattggctgg cggattagac agaggaaata catttgatga attaattcct
    30481 tcattaaaag gaatcaaagc aatggtcgta tttggccaaa caaaagaaaa gttgatggat
    30541 gctggaaaaa aagcaggcat cgagacaata gtaacagccg attctgtaga acaagcagtt
    30601 cctttagcat tagaaaacag tacagatggt gatgtagttt tattatcacc agcgaatgcc
    30661 agctgggatc aatacccaaa ttttgaaact agaggaaaca gattcatgga agctgtgaac
    30721 cgattaaaat agaaaaagta gaagcgagcg ataaaaaatg aaaattttgg ttacaggcgg
    30781 aggaacaggg ggacatattt atccagctct tgcctttgtc aattatgtaa agactcaaga
    30841 accgaatgct gaattcatgt atgtgggagc aaaacgaggt ctagaaaaca aaatcgtccc
    30901 tgatacagga atgcctttcc ataccttgga gattcaagga tttaaacgaa aaatttccat
    30961 gcataacatc aagacggtcc gattgtttct taagagtatc cgtcaagcaa aaaaaatcct
    31021 cagtgatttt caaccagata tcgttatcgg tactggagga tatgtttccg gagcggtagt
    31081 gtacgctgca gccaaaatgg gtattccaac agttatccat gaacaaaaca gtgtaccagg
    31141 tgttaccaat aaatttttaa gccgatatgt ggatcggatc gctctttcat ttaaagatgc
    31201 ggcttccttt tttcctgaaa acaaagcagt tcttgtaggt aatcctcgag cacaagaagt
    31261 agcagatact aaaaaatcag atattttgaa aacatttgat ttggatccag aaaagaaaac
    31321 agtcttgatt tttggcggta gtcaaggagc acttaaaatc aatcaagcag tatcagtttt
    31381 tttaagtgaa tatattcctt cttcttacca agttttgtat gcttcaggag atagatatta
    31441 tgaagagatc ctaaataaaa taaatcctga aatacaaaat attagtgtcc agccttatat
    31501 caaaaatatg gcagaggtca tggcttccag tgatctgctg gtggggcgtg ctggtgcaac
    31561 ttcgatcgca gaattcacag ctctggggtt accggcagtg ttggttccca gcccatatgt
    31621 tacgaatgat catcagacta aaaatgcaat gagtcttgtt cacgcaggag cagcaaaaat
    31681 gattgcagat aatgaactaa caggtgaatc actaagtcag acagtcaacg aaatcatggg
    31741 tgatgaagaa ttgcaaaagc aaatgtgccg agcttctaaa gaacaaggaa tccctgatgc
    31801 ttcaaaacgt ctttatgact tagtaaaaca aataatcaaa aaataaaggc ggtggtctta
    31861 gtgagtaaga aaaaagaacc aaaaaaattg tctcatccgt cttctgatga aaacaatttg
    31921 actccttggc aaaaggctaa ccgaaaatat ctggcagaac aagagatgaa aaaccaagag
    31981 ccaaaaaaag aagacataac attagataca cccctttcag attctgaaga agaaaaaacg
    32041 gttcaagagg aacagcaaga agaaagccag tccgagaaaa aaatcgaatt ttttgaagag
    32101 atcaagcaaa agaaaaacgg tggaccatat aacggttctt ttttgaaccg gctgcccaat
    32161 ttaaaaagtc aacggcacaa agtactattt cgtcggttag ctttgatcat cactgtattg
    32221 ggtataccgc tgattttttt gatctattat gtttctcctt atagcaaatt acaagcagtt
    32281 gcagtatcag gcaataagac agtcaacagc caagaaatca tctctgatac aaaattgtca
    32341 ttaggagaaa atgtttgggg acagtatttt catcgatcta cttatatcga tcgccttaaa
    32401 aaagcacagc caagaatcga aacagcaaat atacatttca aagggatgaa tgaattcgat
    32461 ttggatgtaa cagaatataa agagattgct ttgatcgcaa aaaacaatca atactaccca
    32521 gtgattgaaa atggaacagt attagatgaa aaagtagcaa atcctacaaa aaatctccct
    32581 atcttggaag agttcaaaga ctctgcaaaa atcaaagaat taacaaagca atacaatcag
    32641 ttatcatcag aattacaaaa agcaatttct gaaatcaagt acacgccaag agcaagcaat
    32701 aaaaatctga tccaattgaa tatgaatgac ggcaatcaag ttattgtaaa tatcaataat
    32761 cttgcgaacc aaatgaaata ttattcgcaa gtagccaaag atatggatga aaaaggtgtg
    32821 atcgacatgg aagttggtat cttctcgcat ccttacggta gcactcaaga aacggaagaa
    32881 tcgggccaat cggaagaaac aactgaaagt gaagaaaatg cggatcaaac aaacgaatca
    32941 agtgaagagg cagaaacgca aaaagcacaa acacctggat cgtctagcac aaacggatca
    33001 tctgattcaa atgattcaag tgaaacaagt caaatagaag ataatccaag tacaactccg
    33061 tcaagtttaa atgaaaattg ataaaacaac tttaaaaagt aagaatgtac tttctaaaaa
    33121 ccagtaattt atggtagaat tggaagcagt gaatatatct attaataagt atattttaaa
    33181 acggacgcta gttgttcgct tgtatatgga atagtaagta ggagggaatc ccattcatgg
    33241 caaaaacagg aatgtatgta ggccttgata ttggaacgac atctgttaaa gttgtcgtgg
    33301 ctgaatatat cgacagccaa atgaatatta ttggagtagg aaacgcaaaa tcagaaggga
    33361 ttaaccgagg cattatcgtt gatatcgata aaacggtcca agcgatacaa cgagcagtac
    33421 gacaagcaga agaaaaagca ggtatccaga tcaaaggcgt aagtgtcggt ttgccagcaa
    33481 acatgcttga agtagaaaat tgccagggaa tgattgcagt taacggagat tcaaaggaaa
    33541 tcaccgatga agatgtcaga aatgtggctt cagctgccct tgttcgttca atacctcctg
    33601 aacgtcaaat cgtgtcaata ttgccacaag attttactgt cgatgggttc gagggaatca
    33661 aagatcctcg tggaatgatc ggcgtaagat tagaaatgta cggtctgttg tttacaggac
    33721 ctaaaacgat catccataac atacgtaaat gcgtggaaaa tgccggatta atcgtcaacg
    33781 aaatggtgat tactccttta gcgctgactg aatcaattct ttctgacgga gaaaaagatt
    33841 ttggtacgat cgtcattgat atgggtggcg gccaaacaac aacggctgtt atgcatgata
    33901 agcaattgaa gtttaccaat cttgaccaag aaggtggcga atttgtcact aaagatatct
    33961 ctattgtatt aaatacgtca tttaacaatg cagaagcgtt gaaaataaac tacggggatg
    34021 catacccaga acggacatca ccagatgaag aatttccagt agatgtgatt ggtcagtcag
    34081 aaccagtgaa agtcgatgaa cgctacttgt cagaaattat ttcagctcga atggaacaga
    34141 ttttcaacaa agcaaaagat gcattggacc aaatcgaagc attagagttg ccaggtggcg
    34201 tcgtattgac aggcggagct gctagtcttc ctggtgttgt ggacttggca caagaaatct
    34261 tcggcgtaaa tgtaaaattg tatgttccaa atcacatggg gctgagaaac ccggtattta
    34321 caaatgtaat aagtatcgtg gattattcag caaacttgag tgaagtgtat caattggcaa
    34381 aaattgctgt tacgggtgaa acctcagcag ctcgtcaaat ggtagttgaa caagaaacaa
    34441 caaacacata tgagagctat gaagcacctg aagaaacaat ctacgatgag ccggaaccaa
    34501 aagagtccgg agaaaatgta aaaaataaaa tcaagggttt ctttacaaat atttttgatt
    34561 aattcatcct gggaggaata agatatggaa ttctcaattg ataataatat caatgacggc
    34621 gcagtaatca aagtcatcgg agtcggtggt ggcggtggaa atgctgtaaa ccgaatgatt
    34681 gaagaaaacg ttaaaggtgt tgaattcatt acagccaaca cagacgtgca agcattaaaa
    34741 aattcaaaag ctgaaacagt tatccaatta ggccccaaat atactcgtgg tttaggtgct
    34801 ggttctcagc cagaagttgg acaaaaagcg gcagaagaaa gtgaacaatc cttacgcgaa
    34861 gctttagacg gtgcagatat gattttcatc actgcaggaa tgggtggagg aaccggtaca
    34921 ggtgctgcac caatcgttgc aggtatcgcg aaagaattgg gcgcattgac tgttggtgtc
    34981 gtgacacgtc cattcacttt tgaaggaccg aaacgtggcc gttttgctgc agaaggtatc
    35041 gctcgtttga aagaaaatgt ggatacatta ttaattatct caaataaccg tttattagaa
    35101 gttgtagata aaaagacacc gatgcttgaa gctttccgtg aagcagataa tgttttacga
    35161 caaggtgtac aaggtatctc tgatttgatc acagcgccag gttacgttaa ccttgacttt
    35221 gctgacgtga aaacagtgat ggaaaaccaa ggtacagcat tgatggggat cggtgtggct
    35281 agtggagaag atcgcgtagt agaagcaacg aaaaaagcaa tctcctctcc attgcttgaa
    35341 acatctattg atggagctga acaagtactt cttaacatca caggcggttt ggatatgaca
    35401 ttgttcgaag cacaagatgc ttcagatatc gtagcaaatg cagcaactgg cgatgtaaat
    35461 atcattctag gtacatctat caatgaagaa atgggcgatg aaatccgtgt gacggtcatc
    35521 gcaacaggta tcgatgaatc aaaaaaggaa cgcaagtcta gccgaccagc tagacaagca
    35581 caaatgcaat cacctgctca aaagactgta ttagatatgg atcaagctaa accaacttct
    35641 tcagaagaag aaaacagttt tggcgattgg gatatcagac gtgaacaaaa tgttcgtcct
    35701 agagttgatg attccaattt tgacaatatc gaaaaaaaag aatttgatac attcaaccgc
    35761 gaagaaacaa aatctcatgg cgatgatgaa ttaaatacgc caccgttttt ccgtcgtaaa
    35821 aaataaagga gaggaagagc acatgattgc tgacaacttg cagaaaatca agcaagaaat
    35881 tcaggattcg tgtgctcttg ttcatcgtga tccgaaagaa gttacattag tagctgtaac
    35941 aaaatctgtt gaatccgctc aagctgaaga attggttaga gctggcgtta tgaattgtgc
    36001 agaaaaccga gtagataaat tattagaaaa gaaacaagat ttatttgatt ttcctgaaat
    36061 aaagtggcat ttaattggta atttacagcg cagaaaggta aaattgattg taaatgaaat
    36121 tgattacttt catgcgttgg acagtttaag attagcagaa gaaatccaga agcggttaga
    36181 gaaagaatta tcctgtttta ttgaagtaaa tgtttctggt gaggaatcaa aacatggaat
    36241 ttcaccagat gagcttttac cgtttgtgga atctttagct gcctgcacaa aaatcaaggt
    36301 tgttgggttg atgactatgg cgcctaaaga agcttctgat gagcaaatcc ataaagtgtt
    36361 tggtacgctg aaagaattgc gtgatcttgt tcaagcaaaa ggttatgccc atgcaccatg
    36421 tagtgaatta agtatgggga tgagtcaaga ctttcaaatt gcgattgaag aaggtgcaac
    36481 atttatccgt gtaggaacag cgctattcaa agacgaataa aggaggaaca aaaatgccgc
    36541 ttttaaacag aggagcaatt gctaattttt tcggattagc tgatgaggaa gagtacgaat
    36601 accaagatta tcctgaaaca acaaaaaagg ctgtacagcc aactaaaaaa aatacaacac
    36661 agaatcaatc aactcattct gtatctgctg cgaatactca tgaaaaacaa aaaacggcga
    36721 cttcacgtcc tgtaacgcat gaaagtattc agccggctga aaagaaaaaa gtaattgaac
    36781 tacatcaatc acctacaaaa actgtagcga aagaaagaac agcttcaaga acctctacac
    36841 aacctaaagc aaatcttgct ggaaaaatca ctgtaatgga accaagagct tattctgaag
    36901 ctatggcgat tgctaaacga atcattgctg gagaagcaac cttagtgaat tttcatttag
    36961 tagaagaaaa acaagcgaga agaatcgttg attttctaac aggaacagtt tatgcactag
    37021 atggagatat caaacgtgtt ggagatgaga tttttctttg tacaccgact gggatggaga
    37081 ttgacagttc aaccgctcaa tcttttgcag atgcgaattt gtttgatctg taagaggagg
    37141 aactacgatt tatactattg cactattact tcatttactt caacgggcag cctatttcta
    37201 tagcattatc ttgatggtat atgcattgct ctcttggttt ccaggcgggt atcaatcagg
    37261 gctaggacga ttattagcta agatctgcga accgtatatc agtttgttcg atcgtctgcc
    37321 tttgaggatt ggacccttgg atttcacaat tttaggggca atcctgatct tgaacttagc
    37381 cgtacaaggt ttagcacaga tcgttatatg gataactgtc catttagttt attgattgaa
    37441 gcaacaaaat ctttattctc agaatagata ggaggggaaa attttggatg caaatgtata
    37501 ccagcacttt cgggcagatg aacgtacatt tatcgattcg gtaggcgatt ggattgaaca
    37561 ggttcaagga caatatgcac cctatctgac cgaatttctt gacccaagac aatcctatat
    37621 tttagaaaca ctgattcgac aaagcagtga tttgcgtttc atgttttatg gcggatatga
    37681 acaggcagaa agaaaaagat gtttgatttt ccccgattat tacgaacctg tagaggaaga
    37741 ttttgaaaca gcattgtatg aaatccatta tcctagcaaa tttgccagcc taagtcatgg
    37801 gaaaatcctt ggaactttag tgggaacggg gatcaaacgc tcttactttg gcgatatcat
    37861 ctcggacggt gatcgctggc aagtatttat tgcaaatgag gtggatcatt ttgttgctgc
    37921 acaagtaaca aagattggaa aggttgctgt acgcttggaa ccaatcaaat acacagaaat
    37981 gattatgccg aaagatagct ggacacaaga aagaggaaca gttagttcca tgcgactaga
    38041 cagtgtgatc tctgagattt ataatatttc cagacagcgg tccaagcagc tgatcgaagc
    38101 tggtaaagta aaagtgaact ggacagaaaa tacaagacct gattttgtac ttgaactatt
    38161 agatattatt tcagtcagag gttatggcag agtccagatc caagaacttg aggggaagac
    38221 gaaaaaagaa aagtacaggg tactgttcgg tgtattacgt aaataaaata aaaaggtggt
    38281 ttactcatgg ctttaactcc attagatatc caaaataaga cattcccgac aaaaatgcgg
    38341 ggatataatc aagacgaagt agatgatttc ttagatttag ttgttcgtga ctatgaggaa
    38401 ttgactcaaa gaaatcgaga attagaaaaa gcagtcaaac attctgaaga aaaacttgag
    38461 tacttcaatg aattaaaaga tgctttgaac caatcaatca tcgtagctca agatacggct
    38521 gataaagtaa aaacaagtgc aagcaaggaa tccgaagtga tcgttacttc tgcacaaaac
    38581 aaagcagatg agttagttgc gaacgctgaa aaacgtgcac atcaattaac aactgatgca
    38641 gaagaaaaag caagaaaaat cttgacagat gcaacagaaa aagcaagaca gcttgcgaca
    38701 gaaactgaag atcttaagaa aaaaacacga gtattccatc aacgtatcag cttgatgctt
    38761 gagtctcaat tagaacaagt gaaaagtccg gaatgggatg aaattttaca accattctcc
    38821 agctacgtga ctgacagcca tgaagtgatc aaagaagttt tagcaaaaca gcttgacaat
    38881 gaaaatgata ctgatgtaaa ctcagacaca ggagtcatag aagcacctat gacggcattt
    38941 gatacacagg ctattgatat ctcgatcgtg cctggtgaaa atgaaaacga gtagaacaga
    39001 aaagcacttt gttattctta aagcgagccg atgagagtgg aagttcggta agacactgca
    39061 tggtgaaaga tcctctactt gagacctgct tttccgaaat tagagtaaga agcagcggct
    39121 gacctcgtta ttggtcacag agagtttgca ctagatagat gcaactaaat ttgggtggta
    39181 ccacggcatg ttcgtccctt gcggctgaac atgctttttt gtgtccggaa aacaaataaa
    39241 ggagtgtatg gttgaatgaa aatgaaagaa accttgcaat taggaaaaac aaaattccct
    39301 atgcgtggta atttgccaaa ccgagaaggc gagtggcaaa aagaatggga agaaaatcga
    39361 atctaccaaa aaagacaaga gttgaacgaa ggaaaaccta gctttgttct tcatgatgga
    39421 cctccatatg ccaatggtaa tatccattta ggacattcat tgaataaaat cagtaaagac
    39481 attatcattc gttcaaaatc aatgtctggc tatcgtgcac catatgttcc aggatgggat
    39541 acacatggat tgccgattga acaagtattg gctaataaag gcgtgaaacg taaagaaatg
    39601 tccatggcag aatacttgga aaaatgtcgt gaatatgcac tttcccaagt agataagcaa
    39661 cgagcagatt tcaaacgcct aggagttgca ggagattggg aacatcctta tgttacatta
    39721 gatccttctt atgaagctgc acaagtacgc gtatttggta aaatggctga aaaaggctat
    39781 atttacaaag gattgaaacc aatttactgg tctccgtcaa gtgagtcttc tttagcggaa
    39841 gctgaaattg aatataaaga tgtaaaatct ccatctattt ttgtagcttt caatgtcaaa
    39901 gacggcaaag atattcttga tgaagataca gcttttgtca tttggacgac aacaccatgg
    39961 acattgccag caaaccaagg tattgctgta aatccaaaat ttacgtatgt attggtggaa
    40021 gccgatggcc gtaagtttgt cgtagcgaaa gatttgctag aaacagtcca atcagaaatc
    40081 ggttgggaaa atgtaacagt tctaaaagaa tttgctggac aagaaatgga atatatgact
    40141 gcaagtcatc cattctatga acgtgaatca cttgtgattt taggcgatca tgttacttta
    40201 gacgctggta ctggattagt ccatactgct ccaggacatg gggaagatga ctatatcgct
    40261 ggtagcaaat ataaactatc tgtagtgtca cctgtagact caaaaggatt gtttacagaa
    40321 gaagcacctg gatttgaagg tatcttttat gacaaagcaa atcctatgat cactgatcta
    40381 ttaaaagaaa aaggagcttt gctgaagcta gatttcttta ctcatagtta tcctcacgat
    40441 tggcggacga aaaagcctgt gatctatcgg gcaactccac aatggtttgc atctatcgat
    40501 aaattccgtc agaatatttt ggatgaagta gaaaaagtag agtgggttat cccatggggt
    40561 aaaacacgtt tgtataatat gatccgtgac cgcggcgatt gggtaatttc ccgtcagcgt
    40621 gcatggggag taccacttcc gattttctat gctgaaaatg gagaagcaat catcacacca
    40681 gaaacaatcg aacatgtggc aaaattattt gctgaacatg gatcaattat ctggtttgaa
    40741 aaagaagcga aagaacttct tcctgaagga tttacccacc caggttctcc taatggcgaa
    40801 tttacaaaag aaaaagatat catggatgtc tggtttgatt ctggttcttc tcatgaagct
    40861 gttttacgtc agcgaccaga attatcattc ccagcagata tgtatttaga aggatcagac
    40921 caatatcgtg gctggttcaa ctcaagtatc acgactagtg tggctatcaa tggtgtagca
    40981 ccatacaaat cagttatttc tcaaggattt gctttagatg gtgaaggccg taagatgagt
    41041 aaatctttag gaaacatcat tgcaccagaa aaagtgatca aacaatttgg tgcagatatc
    41101 cttcgcttat gggtatctag tgtagattat gaagcagatg ttcgtgtttc tatggatatc
    41161 ttaagtcaag tggcagaagt atatcgtaaa atcagaaata cgatgcgttt cttattagcg
    41221 aatacagaag atttcgatcc taaaaaagat actgtttcgt acaatgattt acgttctgta
    41281 gacaaatata tgaccgttcg attgaaccaa gtaatcaaag aaatccgaga agaagggtac
    41341 gacaaatata acttcatgca tatttaccgt acagttatga acttcttgac ggttgatcta
    41401 tcgtcattct acctggattt tgctaaagat gtagtttata ttgaagcaga agatgattat
    41461 caacgtcgtt gcatgcaaac tgttttctat caaacagctg tagctttgac aaaattattg
    41521 actcctatca ttccgcatac agcagaagaa atctggtcat tcctgaaaga agaagaggag
    41581 tacgttcagt taagcgagtt tcctggttat gaagagtttg caaaccaaga agaattgatg
    41641 gatacctgga ctgctttcat ggacttccgt gataatgtct taaaagcatt ggaagaagca
    41701 agaaacagca aattgattgg taaatctttg gaagcaaaac taactgttta tccaaaacaa
    41761 caagttcgtg aaatgttaaa agctttggat gcagatattg cacaattatt gatcgtttca
    41821 ccagactact ttgaagttat ggcagcagat gaacaagtgc cagataacgc tatggtattt
    41881 gatgatgttg ctattacagt ggaaaaagca gacggggaaa cttgcgatcg ctgtcgccaa
    41941 gttcgtaaag atgtaggagt agatgaaaaa cttcctcatc tatgcggtcg ttgtgcaaaa
    42001 atcgttgaag acttctatcc tgaagctgta gctgaaggat tcgaagaaaa gtaaaaggga
    42061 aaagggggtg tgacatgttc tttgtcacac ccccttttgt tatcaatcgt ttaattttcc
    42121 acgttcacga taccagttat ctggttgcca gatccattcg aatccgtctt tttcaagtag
    42181 ttcgaaagct gctttaggtc ccattgatcc agctttgtag ttgggaaaat ccggttgtgt
    42241 ttggtcccaa gcttttcgaa tgacatccac gatatgccaa gattgagcga cttcttccca
    42301 atgagagaag ttcgttccat caccattcaa cgcatctaat agtaattttt cgtaagcttc
    42361 tgggctattt tcaaccattt ctgcactatt tctaaattcc aatttgattg gttccgtttc
    42421 aaatccttgc cctacttctt tcccattcaa ggataaagaa aatccttcag ttggctgaat
    42481 atagatcgtt agaacattag gtggaagtgt cgtatcgtca catggttcat cgacagatgt
    42541 tttgaataca ttaacaggaa cttgtttgaa tacgatgttg atccgagtcc ctttttcagt
    42601 caaacgtttg cctgtacgta catagaaagg aacacctgac caacggaagt tatcgatagt
    42661 gaatttgcct gcgacgaatg tttcagtctc tgattgagca tccactttgt cttcttcgcg
    42721 atagcccttg aattgtacac catccaattc accttgagca tattgcccgc gaacgaagtt
    42781 ttctaacact tcttcttcgt tataaatgcg gatagctttc aatgctttga tcttttctgt
    42841 acggatttct ttttcggaaa atgctactgg cggttccatt gcaagtaatg ctacaacctg
    42901 taaaatatga ttttgcacca tgtctttaag tgcgccacta tgatcgtagt agcccccgcg
    42961 atcttctaca ccgaggtttt cgccaaaagt aatttggata ttgtcgatat agcgattgtt
    43021 ccattgagcc tcaaaaatat tattggcaaa acggatagct gagatgtttt ggatcatttc
    43081 ttttcctaaa taatgatcaa tcctgaaaat atcttgttct gggaatactt ctctgatttg
    43141 ctggttcaat tcaaaagcag actggtaatc agaaccgaac ggtttttcta tgatcaaacg
    43201 attgaagcca ttttttgtca taatattttg tgatttcaaa tgagaaacga tcgttccaaa
    43261 aaattgaggt gccatagcca aataatagac acggttaccg tttaattgat agtgtccatc
    43321 taatcggtca gacaattctt ttaatgtctg ataatgcgtt gaatcattta catcatggga
    43381 ttgatagtaa aaatggcttg caaaggtagt tgcttcttca tctgaaggat tcaatgcacg
    43441 gattgtttct ttgacaactt cacggtagtg ctcatcgctc cagggacggc gagcggttcc
    43501 gattacagca aaatgctcgc ctaaatttcc ttttctgtaa aggcggaaca aagacgggta
    43561 caatttacgc tgggctaggt cacctgtacc gccgaatatc gtaaacaata catttttttc
    43621 attagacatg tggatacact cctttatatt ttatcgacaa aaatcgtgct ggccgcctta
    43681 aagctgacgg ggattttctt tctatcattt tggatagtga tcggtccttc ataagctgcg
    43741 atgtcaacga tctcatattc ttcgttgata ttcaggtcaa tagtgactaa gtaatccaac
    43801 aattcttttt catcaagcac tcgtgcgata cgcacttttg taccgacagg ataggactcc
    43861 aatgtttgtc ggcgttcttc atggacaact tcctcttttt taggaatcga tcctccatga
    43921 ggacaatgtt caggatgatt caaatagttt tctaaatgat ctgcaagatg ttccgaagtc
    43981 acatgttcca atacttcagc atcatcatgg acttcattcc agctatagtc taaatgttca
    44041 actaaaaaaa cttcccataa gcgatgtttg cgaatcagac tgctagcttt ttgcagtccg
    44101 cttgcagtca attgtacacc ttgataaggg gaatgttcta caagctcttc tttcacaagt
    44161 ttagaaatca tttcactgac agatgcagct gagacatcta gactagatac gatttgttta
    44221 ttgttgactt tcgttgtatc tccacctagt tctaaaatga ttttcaaata atcttctcga
    44281 tttggtgtca tattcgatca tcctttcatg gtaaaaaacc atcattagta agtgtaacaa
    44341 aaaaaacgtt aattgactat ttttctaaat aaaaaacaaa ggatttttag acgtttttac
    44401 aacaaaaaac gaaagatgat agagaagact cattctttcg tttataaagg atatatatat
    44461 acattgggag ggatctattt ttcagataac aacttatatg aagtgtctgc agattcgtca
    44521 taaagagttt gttcgggata agaaaagata agataaaaac catactgatc acgtacgact
    44581 aaacgatatt catctatttc taccacagat gtgccaagag actgaccgtt gatcaaaaac
    44641 gtgtaatcag atgtaatctg taattgttgt ttttgctggc tatcactaaa gaaccatgta
    44701 ccttgatact ttgagagtcc ttctgacttt tccgcttgtt ttttcttttt caagtaaata
    44761 aaacttgaga gaacggtaat gagtgaacca agaaaataga tcattttttt tatcaccaaa
    44821 tcactccttt atgagaaaat aatcgcatag acatattctt tcttctttat ttcattgtac
    44881 cgaataaaag ctaaaatttc tctaagactt tcgactggtt tttttactgt ttttctacaa
    44941 attatgatag gatacatagt aggaagaaag gtgacataat ttgaagtatc agcaagtgga
    45001 aatagctttt tttattgacc gcccgaatcg gtttatagca cattgtatga aaaaagacgg
    45061 agaaataatt attgcgcatg tcaaaaatac aggtagagga aaagaggtcc tgataccagg
    45121 cgctgaagtg gctattgtat ttgctcctgg acctaaacgg aaaaccgcct atgatctgat
    45181 tgctgtaaaa aagaagggtg actggtttaa tatcgatagt caattgccta atcggctggc
    45241 aattgatggt attttagacg gcacgatcct tttaccagga ttaactggtc catttacggt
    45301 atacaagcgt gaagtaactt atcttcactc caaatttgat atttatttag agacggtggt
    45361 cggacaaaaa gcatttgttg aagtcaaagg tatgacactt gaaaataaag cgattggtgc
    45421 ttttcctgat gcgcccacac tcagaggatt gaaacatata ggagaattaa cgtatgccgc
    45481 acaagatggt tatgccgctt atgtcttgtt tattgctcag ttcgaacatc ttcatctggc
    45541 aacgatccat gaagaaatgc aaccagcact agctgatatg gttcgacatg cgcagcaaag
    45601 cggagttcaa atcctcgcct ataattgtca agtatcggca gatgaggtaa ctgtcaagca
    45661 ggccattcca tttgatgtag atatcccttt tgaagatcct atacaataga aagaaggtaa
    45721 aataatgatc aacttaggta ttatcggaac aaattggatt actcatcaat ttgtccaagc
    45781 agctttaagc acaaaaaaat atgaattgac agcggtatat tctagacatt tggaaaaagc
    45841 acaggaattt ggttctcatt atgaagggga tatcgaattc gcaacagatt tgaagacttt
    45901 tttcaatatt actcatatgg acaccattta tattgcttct ccgaatagtc ttcactttag
    45961 tcaagctaag caagcaattt tggcaggaaa aaatgtgata gttgaaaagc cagctttttc
    46021 gacgccaaaa gaaatggatg aaatcatcca tttggcaaat gaacacaatg tgtatttttt
    46081 tgaagcagca agaaatatcc atgaatcagg ttttaaaaag attgccgatt acttaccatt
    46141 aaaagacgag ataatcggtg caaatttcag ctatatgaaa tattcttcgc gctatgacca
    46201 agtattagat ggagaagaac cgaatatctt ctcgactcat ttctcaggtg gtgcattgat
    46261 ggatttaggt gtttaccttg tatatgcagc tgttgcttgg tttggaatgc caaaagaagt
    46321 ccattatttc ccaagaaaga tttctactgg tgtagatggt cttggatggg ggatcttacg
    46381 ttatgagaat tttgatgtat cgatccagcc aggtaaaatc ggtgattctt atttaccgtc
    46441 agagatttat tttgactcag gaacgctagt gatgaatgga gtcaatgcaa ttgaacaagc
    46501 acaatttcat gatcgcgcgc ataaagaaat cattgatttg gcggtaacag ctgcagaaaa
    46561 tccaatggca gatgaagcag aggattttgc caatgtcatg gctcatccga aagatccagc
    46621 ttggggattg ttgtatgaag agtggatcga gctagctaga aatgtgaacc aagtgattta
    46681 tgatttaaga aaaaatggcg gcatcaaatt cgatgcagat aatgaggaag atttttaatg
    46741 aacgaatttg tacaagtatt tcccgaaaat tggcagaaaa aatatgagga taaagggttt
    46801 atctcacctt ctcttattca acgagcagtt tttcagccac taagtaataa acaaagtata
    46861 gtagcggttt ctcctacagg ttcaggtaaa acactggctt atctttggcc tctactacta
    46921 aacgtggaac caggtgaagc aagtgcgttg gtcatttttg cttcttctca agaactagct
    46981 attcaagtag cagatgttgc acgtgaatgg ggaaaagaca aagagttgaa agtccaatct
    47041 ttggtgggcg gagccaacgt caaacgtcag attgagggtt tgaaggaaaa accagaaatc
    47101 ttgattggta caccaggaag aatattggaa ttgatgaaag caaaaaaaat aaaagcacat
    47161 caagtgaaaa caatggtatt tgatgaagca gaccagctat ttgacgctgg gaacagccaa
    47221 atcattgatc aaattcttca tcaagctcca acagagtatc agctagcttt tttttcagcg
    47281 acagcagatc gttcccttga aagcattgaa aaaatcactg ggaaaacgtt tgagacaatt
    47341 gacgtgacag cagaggacga ttctcgaaaa ggactgagac attacttttt acgtgtaccg
    47401 atacgaaaga aagatgacta cttgagacgt ctcacgcata tagaagattt tcatggtctg
    47461 attttcttta atcaactgac tgaattaggc gtaatggaag aaaaattgct gtatagaggt
    47521 gtaccggtag gaagtctggc ctctgatcag aacaagctac tccgaaaagc agcgctagaa
    47581 caattcaaaa agaaaatggt cagagcactt ttgaccacag atgtggcggc tagaggttta
    47641 gatattacag gtcttcctta tgtagtgaat gcagaagttc cgttaagtga agaagcttat
    47701 ttgcatcgtt cgggaagaac tggtcgaatg ggtaatgaag gaatagtaat tacactcgta
    47761 caagataata atctaagaga tttaaaacgg cttttacgtc catcaaatat aacacttgaa
    47821 gagatttttt tatacggagg taagctgcaa actgagcagc caaccaaaga agatgcagct
    47881 cctgcagtaa ataaaaagta tgcgaaaact ttttctggaa aggagcagaa cccagaatat
    47941 aacaaagaaa aaataagaaa aaaagttaaa aataaatcaa aaaaagagag aaataaagga
    48001 gcaaggcgca aataagcgcc tttttatgct atgattatga aaacgggaac aaaattggtt
    48061 gccagaggag gggttatgtg aaagaacgtc aagaagagat cttgcagcta ttgacagaaa
    48121 accaacaagg attgacagca agtgatgtag ctgagcgctt aactatcgat cgaagcaatg
    48181 caagccgata tttaagtgaa ctatacaagg cccatcatat tgtaaaaacc gctggccgac
    48241 cagtcgttta ctctttgcca acggaaaagt caaaatctga tgaagtgcat gtcgactctt
    48301 ctacgcaagt gacttttgaa acactcgttg gagaaaatga ttctttaaaa gtcagcatac
    48361 agcaggcaaa agctgcgatt ctttatccac cgcgggggtt gcatacgatt atttttggag
    48421 aaactggtac agggaagtcg atgtttgctg aatgtatgta tcattttgca atcgattcag
    48481 agatgctttc tgcagatgcg ccatttgttt ctttcaattg tgcggattat gctcaaaatc
    48541 cgcaactgct ctttggacac atctttggga tcaaaaaagg ggcttacaca ggagcagctc
    48601 aagatagtcc cggtctaata gccaaagcag acggcggtat attgttttta gatgagatcc
    48661 accgtttacc gccggaaggg caggaaatgc tgtttacttt cattgataaa ggtatttacc
    48721 gtccgcttgg agaaagtgca caagtacatg aagcatctgt tcagatcatt ggggcgacga
    48781 ctgaatcttc agaaagtttc ttatctacgt tcaatcgacg gattcctatg gcaatcacct
    48841 tgccgaatct tgcttcacgt tcactggatg agcgttatga aatcatctct ctttttatca
    48901 aacaagaagc gaatcgtttg aatcaacgta tcgatatcga aaaagaagcg attcttgcct
    48961 tcatgcttta tgatgcggaa ggaaatatcg gtcaagtgaa acgagacttg aaactagtct
    49021 gtgcaaaatc ttttttacat taccgtactc atcatgagaa aaagctaatc attcgaaaag
    49081 aagaactttc ccttcaagta caaaaaggat tactgaaaat caaggaaata ccggaacgtc
    49141 tcgaccgatt catggacagc aaaagtcagt atttgacatt tgaaccagga tttgctgatg
    49201 ttgtctggtc acaagatcca gaacgaaaca tgcaggtcta taacgatatt gaggaaaaag
    49261 tcacttctct ttccgaaacc ggtgtggaga acattgatct agagaccctc atttcaaaag
    49321 atgtggatgc ttatttccag acatatgtca aagaattgac taaaagttcg attcccaaag
    49381 atcttttgcc agatgaaatc tggcggttaa ccaatcaatt gtatgatgtg gctgaaaaac
    49441 ggttagaccg tatttataat gaaaaagctc gttttgcttt tgctttgcat ctgcaaagta
    49501 cattggatcg tgtgagagaa ggtcatatga tcgtccaccc agatttgaat aacgtacgga
    49561 aaaattttaa gagggaattc caggtagcaa tcgatctttc aacgatcatt gaagaagaag
    49621 aacaagttga gattcctttt gatgaaatcg gctttattag tatgtttctt tctatcaatc
    49681 taggagaaag tgagcctgtt aaagaaaaca aagtagatgt tctcatattg atgcacggca
    49741 gacagactgc ttcaagcatg ttggagatgg cacaagaact attaggaaca aaagtaggga
    49801 aagcttttaa tatgccactg gttatggaag ttcaaacaat gtatgaagaa gtgttacaat
    49861 atgtcaaaaa ctatcaagag catttgacga atggcctact cttgttaaca gatatgggat
    49921 cattaaacac cttcagtgat ttgatttatg aagagactgg cattcggacg aaagctatca
    49981 cgatgacaag taccatgatt gtgatagaag cgatacggat ggcaagtgtt ggaagaagct
    50041 tagaagatat ttatcaaaat atccagcttt cctttgaatc gatcgtacgt gatcagttca
    50101 aagcggaaaa gtctgaaact ttacagcgta aaaaagccgt aatcgtgaca tgctttactg
    50161 gtgaaggagt agctgccaaa ttatatcagc gtattatgcc agtgattaat caaaataagg
    50221 tagaactgat ccagatgcag tttttagaac gtgaatcatt caaaaaacac atcgatgcct
    50281 tattggaaga atatgagatc aaagcaattg cgggaacagt tgatgttgat tatcaaaata
    50341 tcccattttt ttcagcctat gacgtttttg atgatgaaaa actaaatatt ttaaagcgta
    50401 tagctgatga tgagatcccg acagctaaaa ttgtcgattc acttgctgga tcattaagcg
    50461 atgtcttttc tattgatgaa ctgatccgtt cattgcaaaa gacagttcag cagatccaga
    50521 ccgatttgtc gattatcgtg gaacctggag tagatactgg aatcgttatc catttagcct
    50581 tcatgttaga cggattgatc aaacaagaaa gccatcggca atttgagaaa ctgtctattt
    50641 tctcaaaaat ttatcgtttg gaaatggata tcgttcgggc aaacttgatg gcactagaac
    50701 gaaaatatca ggtaaaaatt ccagaagatg atatcgcatt tcttacacaa atgtttcttg
    50761 aaaataaaat aaaaaaatga acacactaat catactgttt acacactaaa catctgtgtg
    50821 cgcactataa aaaagagcta aaaagcatga aaacgctatt tcagctcttt tttttattgg
    50881 catgcttctt gcgttatata aaagtgtaaa aaaataaaag gagtgttaaa aatggcaaaa
    50941 aaaacaatca tgttagtttg ttcagcaggg atgagtacga gtttattggt cacaaaaatg
    51001 caaaaagcag cagaagaaaa agggatggaa gccgatattt ttgcggtctc tgcctcagat
    51061 gcagataata atttagaatc taagaatgtc gatgtattat tattaggtcc tcaagtacgt
    51121 ttcatgaaag ctcaatttga acaaaaactt gcgcctaaag gtattccatt agatgtcatc
    51181 aatatgcaag actatggcat gatgaacgga gaaaaagttc ttgcacaagc agaaaatcta
    51241 atgaaataaa tcaagtatgc agttgggggt ataacaacgt ggaagatcaa caaaatctag
    51301 aagccatcat ggggttaatc atgtttggcg gtaatgcaaa gagcgatgca atggaagcga
    51361 ttgcggctgc taaaatcggt gattttgagt tagcggatag gaaaatcgct gatgcagaag
    51421 aatcacttat acaagcgcat catgcacaaa cagacatgtt gacacaagaa gcacaaggaa
    51481 atcatatgca agtgacatta ttgactgttc acagtcaaga tcacttaatg acatctattg
    51541 cgtttactga tttagcaaaa gaaatcatcg aactttatcg tcgcgtatac agcgaataaa
    51601 tacaacttct tttttgaacg gaagaagaaa aatttttgaa cggatttttt attttgttat
    51661 aaggagaact ctctttatat aaaaaactta taggaggaag taaaaaatga acggactatc
    51721 aagttggatg gagaaataca tcctgccggt tgccgcaaaa atcggagcgc aaaaacattt
    51781 agttgctttg cgtgatgctt ttatcggcat actgcccgca acaatggcgg gatcagtcgc
    51841 tgtaatgatc aatgcaatta ttcgtgattt accgcaacag tttaacagta cctatgctga
    51901 cagtttagca gcagacaaag gaatctttgc cgtattgaat gtcatcatcg gaatcaatgg
    51961 ttttgtctgg aacggtacac tagcaatcgc cggattgatc tttgcctttt cttgggggta
    52021 taatcttgcc cgagcttatg gagtaaatga tctagctggg gggatcgttg gtttagctac
    52081 cttgattcaa ggtatttcct ttggtccagc attatcagca gcattagatg tgaatgttcc
    52141 ttcaaatgtc gcagatgcaa tcaatagttc tgagattggt gcaacgattg cagatggcac
    52201 actgtcagtt ggattatggg gttggctaaa actagatcat ttgaatggaa acgcttattt
    52261 tacagttatg atcatgggag ctatttcagt cattattttt tgtaaattga tgcttgccaa
    52321 cattacgatc aaaatgcctg attcagtacc accagcagta tctaaagcat ttgcagccat
    52381 tttaccagcg acgatcgctt tatacgtcat tgctattatc aattatatcg taggtcgtgt
    52441 agctgatggg caactgatca ttgatttggt gcaaaaatat attgcagaac cattcctagg
    52501 gttatctcaa gggttgggag cagtattgat cgtcacagta tttgttcaag tattctggtt
    52561 ctttggtatt catggatcaa atgtattggc accagtgcta gagggaatct ggggacaagc
    52621 acaactaatc aatattgata ttttccaaaa aggttataac ggaaaaactg gtacaccagc
    52681 cgtgttagat gcaatcgaag atggaaaagc ttatatgtgg gtccgcggat cgttcgatgc
    52741 ttttgcatgg tttggcggat caggcggaac gatcgtctta ctgatagcta tcttgatttt
    52801 ctctaaacgt gcagattatc taacagtagc taaattatcc attgggcctg gtatcttcaa
    52861 tatcaacgaa ccagtgatgt ttggtctgcc aatcgtattg aatgcaatca tgtttatccc
    52921 attcttgatt gctccagtag tagcaacagc aattggatgg acagctactt atcttggcct
    52981 agtcgctccg gtatcgcagc aagtcacatg ggtagtaccg ccgttcttat tgtcattctt
    53041 agcaacagga gcggattggc gggcaccgat cgtaacatta gtatgtatgg tcgtgacttt
    53101 cttgatttgg gcaccatttg tcatctctgc aaacaaaatg gaccctgccg caggcgaaca
    53161 acaataagat gaacgtgagg agcgggacgc tttaagtttc gttcctcata atgaattttc
    53221 gaggaggaac tgaaatgatt acaattgcca gtgcaatgca acaagatgaa atcaaagaat
    53281 tgttagaagg atacaatgaa gaaggaatga cttttacatt taaagaaaaa caaggaatca
    53341 agctgttctt tgaaacaagt gcagaagatc cggaagttgc ggctaaaaaa gcaaaggaac
    53401 taatcaaagc acagccttgg ggaacagtcc tttattttca atcaacagct gtttagaaaa
    53461 tggtttaaaa agataggatg gttgagatga taaaaaacgg tttattttta tgtagtattg
    53521 gttctttgat cgtgttgtat agtttgaatc cacattcgca aaggtatgat ttgtatagta
    53581 tggtcacagg tctttttcta acaggattag gaatctattt cttcatgaag gggaaaaaaa
    53641 gagaagaagc aaaggagaaa aataactaat ggaaatcacg aaaaaattaa atattccagc
    53701 atcgtttttt tacgataaag ttatggactc tgtccttttc gatgtacgta aagcgaccgg
    53761 caaatcagta actagaaaac agctgaaaaa tttggagtac gtcaaacaat tctcgcaaaa
    53821 taaccgggct cgcataaaaa tcgaagaagt cattgaaaac cagtcttata agtttcgtac
    53881 atcgacaacg aagaatgaat tcgtcgttca ttatcaaatc aaaccattgg acgattcgac
    53941 ctgtgaggtt cattacacag aaaaaatgga gtcttatggc atgcttcaaa agttgaatga
    54001 tatgctttta ggaacaatct tgatgtattt taaacggaga cgtttcaaaa aaatgctaga
    54061 tatgatcgaa gcaacgtatt aaatcaatga aacatggtac tatttagtta ttgaaaaatg
    54121 aagtaagcta gaaggaattt ttcataggaa gatagatctt tcctgcgaat cctcactagc
    54181 ttttttgtag gaaggggcag caaaatgaca aacaaagtaa gagagctagg tgtatcttcg
    54241 attgaggaaa tgtttgattt agccacttat gcatttaatg gggcagatac acctgaaagg
    54301 cgagaacgtt ttcgtacact tgcggagaat tcttggaatt acggattttt tgatgaggag
    54361 gatcggctaa caagtcaagt catggcgacc ccttttccag tgaattttta cggacaagag
    54421 tacctaatgg ccggcattgg ctatgttgct tcttatccag aagcaagggg acaaggcggg
    54481 atcaatcaga tcatggaaaa gatccttgaa gattgccgtg atcgtaaagt aagcttatct
    54541 tatctggcgc cattctccta tcccttctat cggcgctatg gctatgaaca atcatttgat
    54601 aaaatcagtt atcaactaaa cagtcgtgat gtgccgttta tcaaaaaaaa atctggaaaa
    54661 ataaagcgta tggattttga agatgcaaaa gctgcaatca gaagaattta tgaacagatg
    54721 ccggaaaacc aacgagctgg tctgaagcgc gaagactggt ggtatacgta taaattcaag
    54781 caaaaaggca attaccagtt tgctttgcat tttgatgata atgaggaagc aacgggatat
    54841 atcgtttatc agcttgcaac gtcttccttt attattgcgg agtggggatt tttggatcat
    54901 gatgcttttt gcagccttgt tcgcttcgta agttctcaca atggtgcttt tgaaacattt
    54961 gaatattcat gcggctacgg cggtaatgat caaaattatc tattagaaaa tccatttgct
    55021 tctgtaatga tcacacctta tatgatggcg aggatcgtag atatttcttt gtttttggaa
    55081 aattatccat ttaaaaagag acaagctgca tttgccctac aaatagaaga agatcattat
    55141 gcgaaatgga atgaaggaat cgtggaagta acaatcgaaa atggaaaaaa tcatctctca
    55201 aaagtagaac aaacaaaact cccaatcgtt cgaggaagta tccaacatat cacgcagcta
    55261 ttattagggt atcgtaaagt ggaagagttg gtattccaag aaagaataca ggtgacaaaa
    55321 gaatatgaag aaacgttaag ctcaatcttg ccacagcaac agccaattct taatgactat
    55381 ttttgataaa aaacgggtga tgacaatatt tttgtcacca cccttttgtt tttcatttgg
    55441 catttgaagg aaaatgttaa agtaaatcct cttttttcgt aaatttgaat tttttgtttt
    55501 cttttgtttt agggttttct tttttgtggt cttggattgc ttctttcgta atcttttccc
    55561 gatttttttt agatgaatct aagcgttctg cgtatacttt ttcttttggc ttatcattca
    55621 ttggaaagga cctccctaca ttatttcatt ctacctctag tttatcatct gttaaggact
    55681 agtggaaacg ttttgcctaa cttataagct gattttttta gcagataagc ttgcaagtaa
    55741 aaaacaaaca agctacaatt gatttattga aattgttcct gtagctcagt aggatagagc
    55801 aatcgcctcc taagcgatag gtcgctggt
//
LOCUS       DANKHL010000002        46242 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000002 DANKHL010000000
VERSION     DANKHL010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.002"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..916)
                     /locus_tag="PW101_000061"
     CDS             complement(<1..916)
                     /locus_tag="PW101_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDL2512497.1"
                     /translation="MERKGIFIKVFSYTIIALLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVA"
     gene            complement(919..1578)
                     /gene="vanR"
                     /locus_tag="PW101_000062"
     CDS             complement(919..1578)
                     /gene="vanR"
                     /locus_tag="PW101_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HDL2512498.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            complement(1791..2009)
                     /locus_tag="PW101_000063"
     CDS             complement(1791..2009)
                     /locus_tag="PW101_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512499.1"
                     /translation="MRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            complement(2196..2363)
                     /locus_tag="PW101_000064"
     CDS             complement(2196..2363)
                     /locus_tag="PW101_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512500.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            complement(2521..2706)
                     /locus_tag="PW101_000065"
     CDS             complement(2521..2706)
                     /locus_tag="PW101_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL2512501.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            2851..3222
                     /locus_tag="PW101_000066"
     CDS             2851..3222
                     /locus_tag="PW101_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2512502.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            3480..3809
                     /gene="mobC"
                     /locus_tag="PW101_000067"
     CDS             3480..3809
                     /gene="mobC"
                     /locus_tag="PW101_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HDL2512503.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            3770..5098
                     /locus_tag="PW101_000068"
     CDS             3770..5098
                     /locus_tag="PW101_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL2512504.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            5080..5466
                     /locus_tag="PW101_000069"
     CDS             5080..5466
                     /locus_tag="PW101_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HDL2512505.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            5649..5885
                     /locus_tag="PW101_000070"
     CDS             5649..5885
                     /locus_tag="PW101_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2512506.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(6185..6388)
                     /locus_tag="PW101_000071"
     CDS             complement(6185..6388)
                     /locus_tag="PW101_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HDL2512507.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMLVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(6392..7336)
                     /locus_tag="PW101_000072"
     CDS             complement(6392..7336)
                     /locus_tag="PW101_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512508.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            complement(7337..11257)
                     /locus_tag="PW101_000073"
     CDS             complement(7337..11257)
                     /locus_tag="PW101_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HDL2512509.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            complement(11406..13487)
                     /locus_tag="PW101_000074"
     CDS             complement(11406..13487)
                     /locus_tag="PW101_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HDL2512510.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            complement(13484..14713)
                     /locus_tag="PW101_000075"
     CDS             complement(13484..14713)
                     /locus_tag="PW101_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HDL2512511.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            complement(14703..14954)
                     /locus_tag="PW101_000076"
     CDS             complement(14703..14954)
                     /locus_tag="PW101_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HDL2512512.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            complement(14977..16968)
                     /locus_tag="PW101_000077"
     CDS             complement(14977..16968)
                     /locus_tag="PW101_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2512513.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            complement(17000..19399)
                     /locus_tag="PW101_000078"
     CDS             complement(17000..19399)
                     /locus_tag="PW101_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HDL2512514.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            complement(19329..19751)
                     /locus_tag="PW101_000079"
     CDS             complement(19329..19751)
                     /locus_tag="PW101_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HDL2512515.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            complement(19765..20301)
                     /locus_tag="PW101_000080"
     CDS             complement(19765..20301)
                     /locus_tag="PW101_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HDL2512516.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            complement(20338..21201)
                     /locus_tag="PW101_000081"
     CDS             complement(20338..21201)
                     /locus_tag="PW101_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512517.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            complement(21293..21508)
                     /locus_tag="PW101_000082"
     CDS             complement(21293..21508)
                     /locus_tag="PW101_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HDL2512518.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            complement(21585..23393)
                     /locus_tag="PW101_000083"
     CDS             complement(21585..23393)
                     /locus_tag="PW101_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDL2512519.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            complement(23390..23866)
                     /locus_tag="PW101_000084"
     CDS             complement(23390..23866)
                     /locus_tag="PW101_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HDL2512520.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            complement(23941..24153)
                     /locus_tag="PW101_000085"
     CDS             complement(23941..24153)
                     /locus_tag="PW101_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDL2512521.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            complement(24175..25140)
                     /locus_tag="PW101_000086"
     CDS             complement(24175..25140)
                     /locus_tag="PW101_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2512522.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            complement(25186..25374)
                     /locus_tag="PW101_000087"
                     /pseudo
     CDS             complement(25186..25374)
                     /locus_tag="PW101_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(25631..26152)
                     /locus_tag="PW101_000088"
                     /pseudo
     CDS             complement(25631..26152)
                     /locus_tag="PW101_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            complement(26168..26866)
                     /locus_tag="PW101_000089"
     CDS             complement(26168..26866)
                     /locus_tag="PW101_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL2512523.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(26823..28484)
                     /locus_tag="PW101_000090"
     CDS             complement(26823..28484)
                     /locus_tag="PW101_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDL2512524.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            28702..30102
                     /locus_tag="PW101_000091"
     CDS             28702..30102
                     /locus_tag="PW101_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HDL2512525.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(30354..31433)
                     /locus_tag="PW101_000092"
     CDS             complement(30354..31433)
                     /locus_tag="PW101_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2512526.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(31519..32478)
                     /locus_tag="PW101_000093"
     CDS             complement(31519..32478)
                     /locus_tag="PW101_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL2512527.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(32490..34001)
                     /locus_tag="PW101_000094"
     CDS             complement(32490..34001)
                     /locus_tag="PW101_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL2512528.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(34023..34586)
                     /locus_tag="PW101_000095"
     CDS             complement(34023..34586)
                     /locus_tag="PW101_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDL2512529.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(34592..35455)
                     /locus_tag="PW101_000096"
     CDS             complement(34592..35455)
                     /locus_tag="PW101_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2512530.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(35469..36365)
                     /locus_tag="PW101_000097"
     CDS             complement(35469..36365)
                     /locus_tag="PW101_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2512531.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(36540..37829)
                     /locus_tag="PW101_000098"
     CDS             complement(36540..37829)
                     /locus_tag="PW101_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2512532.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(37801..39318)
                     /locus_tag="PW101_000099"
     CDS             complement(37801..39318)
                     /locus_tag="PW101_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL2512533.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            39516..40406
                     /locus_tag="PW101_000100"
     CDS             39516..40406
                     /locus_tag="PW101_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HDL2512534.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(40479..41303)
                     /gene="nadE"
                     /locus_tag="PW101_000101"
     CDS             complement(40479..41303)
                     /gene="nadE"
                     /locus_tag="PW101_000101"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDL2512535.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(41315..42787)
                     /locus_tag="PW101_000102"
     CDS             complement(41315..42787)
                     /locus_tag="PW101_000102"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDL2512536.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(43424..43786)
                     /locus_tag="PW101_000103"
     CDS             complement(43424..43786)
                     /locus_tag="PW101_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDL2512537.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            44009..44761
                     /locus_tag="PW101_000104"
     CDS             44009..44761
                     /locus_tag="PW101_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2512538.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(44901..45824)
                     /locus_tag="PW101_000105"
     CDS             complement(44901..45824)
                     /locus_tag="PW101_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2512539.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(45839..46111)
                     /locus_tag="PW101_000106"
     CDS             complement(45839..46111)
                     /locus_tag="PW101_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512540.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        11537 a        11336 c        10320 g        13049 t
ORIGIN      
        1 cggcaaccgt gcgccctatg tccaacggtt cagctatggg tacgattctc ccatcattca
       61 ggctgacaag ctccagtatt tcggaaatga ttttgccctg cctatccatc attttgatgc
      121 attcgcgcag atacttagaa tgatctttgt agtcaccgat attttcaagc attccctcca
      181 acagaacgct tgtagccgcg atgggcgttt ttagctcatg agaagctgcc gcaaagaaat
      241 atcgctgtgt ttcctccaac tcatgttccc ttgcgatttc atcctccagc cttgcgatgg
      301 tttctttcag cctgacatac atggaatgca tatcatgagc cagtgcgcca agctcatcct
      361 ttcgctccag cggcggcggt acatctttca ggtttgccat tttattcgca ctgtccgcta
      421 aggctttgat cggcgttgtc atttgccgcg caaagatata cgcgcataaa aggctgaata
      481 caaccattat cgcaatcata acaattcccc gaattgtcag cccttgataa agcaatccca
      541 cacctgcctt gctttgagca acaatcgaaa tattatcatc tctatgtacc acataaagaa
      601 agtcgggtct aaaactattt gatgtattgg catttggtgt ggcatagagt acgcttccct
      661 ctttatcttc aatataaaac tcaaaggatt ggttattgta gtgaaacagc cctgccacct
      721 cttgaatatc aagcctatcg ctattctgaa tcagttccac caatggctga taggatttta
      781 ctgtttgctg taattccatc actctgaaat aagacacaaa ttgctgtgca aacagtgttg
      841 ctgttacacc gacaagcagt agcagggcaa tgatcgtata ggaaaaaacc ttaatgaaaa
      901 tcccttttct ttccattatt atgattcctc caatcggtat cctacaccgc gaatggtttt
      961 gatgatattt tccggcagct tggcccgcaa atttttgata tgagtgtgga ctgtgctgcc
     1021 atcaccctca aagtcatatc cccatatacg tgacaaaatg atttcatgcg ttaaggttct
     1081 gcctttgttc tgcaccagca gcaaaaggat ttcaaattct tttcgtgtca ggggcagctc
     1141 tgtaccgtca caaagtaccg tgaaatcctc cggcagaagt gtcagcctgc cgacacggat
     1201 ttcctttgcc agcgcaccgc tgcgccgcag cagggcttcc acccgcttta gtaaaatctg
     1261 catcttgaat ggctttgtta catagtcgtc tgcctctgca tcaaacgccc ggatttggtt
     1321 ttcgtcatcc gacagggctg tcatcatcag aatgggagta tcatttttcg cacgaaattc
     1381 acgcaaaagt tcatgcccgt tcataccggg cagcataata tcaagaataa ccagttgata
     1441 agtgttttcg taaaacttgg tgtatgcctc atttccatct gtgcaggcat ccacctgata
     1501 tcctgcctca gccagaaacg cccttactgt attacagata tgatcatcat cctcgacaag
     1561 tagaattcgt atcgacataa aaatccctcc attttaagaa gataacataa cagtctgtag
     1621 tttccctgta gtttcaaagc gatttctttt cggattttaa tcatccgtca ttgggatact
     1681 cgcttctttt catggtgtat gtatctatat tattggttta aacgaattaa atcaagataa
     1741 taataattaa caccttctta atgtttggga tgtttcgatt acagcggttg tcaaaacatc
     1801 ccgataatac tggcaagcaa tggatcagaa tatcctttac ggatattctg atccaccact
     1861 tgcccgcctg cggcgttctc gttttgcttg ttggggacac cccaagcctc agcaaaatgc
     1921 gcggcctctt ttttgcgaat gcaccttgcg gtatggcttt tcacacaggc agggcctgtg
     1981 tgtactccct ccggtcgctc ttgccgcact ccgcgcagca gattcagtcc taataccgtt
     2041 caagacagta gaaaatcagc aagtctatat tcctcatatc ttcatcatcc gtgtccttgc
     2101 agttacagga aatgtaataa tcgccaaatg taaatcccag aatattttcc tcaaaaagcc
     2161 gtacctcccc ggtattaaaa tcaatcaccc catactcaaa ggcgcattag accagcagtt
     2221 atccaatgtc tggagacttc accataaaga atccagatgc cggccttttc aattctacca
     2281 tatacggctg atacagcagc agcttcctcg tctttgtttc gggcattttc catttctgtc
     2341 atgataaagt ttcgcttctt catacatcca tgtgtaaatc ttttctttct gtttcagatg
     2401 agaatacttt ttatcggtct ggagcagcct gccatctatc atcttatagt ttttccgtga
     2461 caaaatacca tgctttctct ctcttcaaaa ccatatccaa aatgcgccgg tttcattata
     2521 ctattcagta accgcctttc ctttctttgt acgcctgtca atcggctttt ccgcagtttt
     2581 gggaatgagc cagatcgtcg ccatctttac agcgccgggg atgcgtcccg cagcacaata
     2641 gtagtttatc catcgtgggg gcacacccca tttctcgcct gcctctttca acgtcatgta
     2701 gtccatagta cacctccaca gggtacatta taattctttt gctcgaagaa taaaagaaac
     2761 aaaatgtgaa atataaactt taagaaaggt gcatatacca acattccacg ggcagagtat
     2821 gaaatataca atgtaagtgg gaggtgcgat atggcgaata ttgaagattg tcccggcttt
     2881 gaaactttcg gcgcggatgt gaaagcggcc cggcaagcaa agcgcgtttc gcgcaaagca
     2941 atggcagaga aaataaatat tgactggcgc tatcttgcca acatcgaaaa cgacggggcc
     3001 attcccagtc tgccggttgt catacagctt attaaagcct gcgggcttcc tgtggaacgc
     3061 tactttaacc cggagatcat gcgggaggaa agcgaactgc ggcaaagggt cagccacaag
     3121 ctgaaactct gccccgaaga atacctgccg atcatcgagg gcgcgattga cagcgccata
     3181 ctaattgaaa agccgaagaa gaatgaaacg gaggatgtgt aactggcatc ctctgtttta
     3241 cttttagcgg gagctgcaca gcgtagcttc tatttttttg cacttttttc tttccagagg
     3301 gctattttgc gggtagtttg gggctttgga aacgtcttgt tagtggagaa acagtaacgt
     3361 agcaagcata gggagtgtag ccactcccgg aaaaacggcc tgccccgtcc ctgccgggcc
     3421 gcgtccgttt tgcttgttgg ggaaatcccc aaacccctga atggaaagga cggtgaagca
     3481 tggcaaacag aaagcgaaaa atcgtgctgc gcgtccctgt gacgccggag gaacgggcta
     3541 tgattgaaca gaaaatggcg ctgctgcata cgaagaactt ttcagcctac gcccggaaga
     3601 tgctcattga cggctatatc gtcaacatgg acaccagcga catccgggcg cagaccgccg
     3661 agatacagaa gatcggcgtc aacgtcaatc agattgcaaa gaggctgaac ggtatggggc
     3721 cggtgtacgc acaggacatc gaggatataa agggggcgct ggcgcagata tggcagttac
     3781 aaagatacat cctatcaagt caacgctgaa aaaagccctt gactacatcg agaacccgga
     3841 caaaactgac ggcaagctgc tggtggcctc ctttgcctgc tcttatgaaa cagcggacat
     3901 tgaatttgaa atgctgctgg cgcaggcata ccaaaagggc aataatctgg gccaccacct
     3961 gatacaatcc tttgcccccg gcgaagccac gccggaacag gcccatgaga tcgggaagca
     4021 gcttgccgag gaagtgctgc aagggaaata tccctatgtg ctgactaccc atattgataa
     4081 gggccacgtc cacaatcaca ttattttctg tgccgtggat atggtaaatc agcgcaagta
     4141 catttccaac aaacagagct atgcctatat ccggcgcacc agcgacaggc tgtgcaagga
     4201 aaacggattg tccgtggtga agcccggcca gagtaagggc aagagctatg ccgagtggga
     4261 cgcccagcgg aaagggacga gctggaaagc gaagctcaaa gcagccattg acgccgctat
     4321 cccgcaggca aaagattttg atgatttcct gcggctcatg caggcgcaag gctatgagat
     4381 caagccggga aagtttattt ctttccgcgc tcccggacag gaacgcttta cccgctgcaa
     4441 gacattgggg gaagcctaca ccgaggaagc catcaaagag cgtatcaaag gccgcgttat
     4501 caccagagcg cccaaagagc gcaaggggat ttctttgcgg attgatctgg aaaacaacat
     4561 taaggcccag cagtcggcag gctatgagag gtgggcgaag ctccacaatt tgaagcaggc
     4621 cgcaaagact ttgaatttcc tgactgaaca tgggatagac acctacccgg atttggaaag
     4681 caaagtggcc gagatcaccg ccgccagcga cgaggccgcc gctaccctca aagctatgga
     4741 acaccggctt gcggatatgg cggtgctgat aaagaatatt tctacctaca agcagctctg
     4801 gcccgtggct atggaatacc gcaatgcagc ggataaggca aaattccggc gtgagcatga
     4861 aagcacgttg attttgtatg aagctgcggc aaaggcccta aagggacagg gtgtgaaaaa
     4921 gctccccgat ctgtacgccc tgaaagccga gtacaagcga ttggccgagg aaaaggaacg
     4981 gctgtatgag aagtacggcg aagcgaaaaa gcagatgcag gaatacggca tcatcaagca
     5041 gaatgtggac ggcattttgc gtatgacgcc gggaaaggaa tggacgcaag agctatgagt
     5101 attccacgaa tgaattaccc tcaatatcgc aaggcccgga agctgacgca tgagtgctgc
     5161 aactattgtg acggcaactg cctgttgctg gacgacggcg aagaatgtat ttgcgtacag
     5221 agcatttcct attcgcttct gtgccgctgg tttaaggccg ctgtcctccc gctggatgcg
     5281 gccctgtatg ccgagatcac caaagaccgg gacgaagtga aacgctgctg tgagtgcggg
     5341 gccacgttca cgccgaagtc taaccgggcc aaatactgcg cggcctgctc caaacggatg
     5401 cgccgccgca aagaggccga gcgccagcgg aaaagatact ggaaacctac gcacttagag
     5461 ctgtaaaagc ccttatattt caaggctttt ccggcatggt cagcggggta tgtgggtgtt
     5521 ttatcctatc cccgccgaag tgctgttcta aatgcgtaga atttaccagc agaaaagcgc
     5581 cgttttctct ggttccatgc ttccgaatat ggtatcatag aaaaaatcag tctgacgggg
     5641 gtatcaggat ggacggacaa gaaaagctgc tggactatga aacgatcaaa gccgccgttg
     5701 ccggtgagag gtgggccacg gaaaaagtgt tggcccatta tgctgactac atcaacgagc
     5761 tttccacggt ggagatcaga caggcaaacg gcaaaaagaa gaaagtcatt gacgaagata
     5821 tacggcagcg catttccctg aaactgctgg aagcattgcc gagcttcccg ctggaacaga
     5881 gatagacggc aagagggctg caccctctta cgccgctttg cggctatcac ctgcacaccc
     5941 ctgaaacaga tacacccgct gcgcggctat atctgtttca ggggtctaat tatagctgca
     6001 acctacgcat ttaagggatt gaaaaccgcc cgctgcaagg cttctcccgc atgtttgcag
     6061 gggcaagcga atacttcatc ctatccccga caataccggg ttctaaatgc gtaggaatag
     6121 gcaaaaagaa aagacgccgg acgcaagcga ccacatggcc gccgcgcccg gcgtctgctg
     6181 ttcgttattc gtctgtgaag tggaacttct ggccgtcata gtcggcatcc gtcatgcgtt
     6241 ccagcttgga aagggtctgc tgggcctgcg cccgcatatc cccatccata accagcatga
     6301 cggcggtgag cctgtttatc gttctgcgcc ggtctgcatt gtggtacata cagatcaggt
     6361 tttcttcctc cacggtaaag tgcatcattc cttatacctc caattcattc tttttggttc
     6421 tggccgccgc cttttttggg ctggcggcct gtttgctgtc ggatagctgc ttgcggatag
     6481 agggccgggg cctgcgaata ctcacatccc ggcgctcgtc ccggttaatc acggcataca
     6541 cgccatgcct gcctttcatc ccggtaaaac acagggattt atagggcagc atggcaaaga
     6601 gccggtcatt atccttggtg gaagcaatcg ccgcaaagtg cggggagatc tccaccatga
     6661 aatgcgtttt attggggctg ttaggcgcgg acagatcttt caggctgtcc acaatacgcc
     6721 gggcctcggt ttcaatccgg ctgtcatgca aagcggtgac gtggactttg aaatcgccgg
     6781 ggacggcccg cccccggctg tgctgctccg tccgtagcgc ctcctgcagc acagccgggt
     6841 catggggctg ggcctcatag tacaaaaagt ttcccaatac ccggtcaggg cttccgtcaa
     6901 aacgctggct ctccgggatg tcacgggtgc catgctcata aaagagccgg ttcacactca
     6961 ccggctgggc ggtttcaatg acgtgccgga aatgctgcgg atagtccagc tcatagagat
     7021 tgccccggat agtgccgccc tgtgtgccgg tcagctccac cgcgtaggcc agtatcttgt
     7081 cacgggtctg ctccccgtaa aatttccatg tgttgtactg tcgggtgtct ttcaggaaaa
     7141 catcacgttc ccggaaacag tacgtcccgg agggccggga catccagagc agggtcttgt
     7201 ctgcggcctg atcgctggcg gcatcccgcc tgataatgtc tttgtcaatg ccaaagtcac
     7261 tttgataaaa cgcggtgttc tgccgcatga tctgttccag cgcggccaga acgtccacat
     7321 tttcaaactt ctgcatttac tctacctcca atccctttga tttttcccgc gccggtggct
     7381 gcttggcctg ttcggtcttg gccgcgttga gctgcgcccg gatagagggc ttttctttct
     7441 ccctgcgggc cgcaagctgt aaggcgctgg gcttttcgcc ggaaaggggc tggatgcact
     7501 tgatacggtc agcgacgcgg attgtggtgt cgtttaactg ccgctgccat gccccggcct
     7561 tgggcgacca gcggaaaccg ttgcttttca gctcgtcgcg tacagcagca tccggcttcc
     7621 cctcaaagaa gatttgcagg cggttggcca cccggttgat ctccacagtg ccgccgtcaa
     7681 actcccatcc cacataggcc gcctcctttt ggcgggtcaa ttcctcaatg cggcccttta
     7741 accggcgtat ctcggcgtta ttgttggaga gctgccagga ctggaacggt tttttctcat
     7801 agtgccagcc cgacgccata tccgctttca ggttttcaat gcctctttcg gtcagatggg
     7861 ggcatccgtc cagcgtccca tgcttgcggt agtaggcatt gacggccttc atcaactcct
     7921 gcgacgcttc cagcttttcc agtttctttt ccagcttgga aacggcctgc ggatcgtcgg
     7981 cgctgatacc acccatgccg gtgctgcgga ttttgtcaag caagccctga atgtactgaa
     8041 attcctccat gttgcggtca ctggcggcgt tctgcttttc tttcgcccgt accgggaaat
     8101 tggacggccc ggcaatcagg acagagggga cgcgggcggt aatctcatag cccttattca
     8161 tgttctcggc cagcttgcgg gcgtaggtgt caaggaggct gtctatcttt tcgtgatact
     8221 ctggctccac acgctttttc tggcgctccg caatttccac ggcctgatcg acatagtgcc
     8281 ggtattccgc cgtggcgctg ccgggccggt agtcagaaaa gctcatggct tccttggcgc
     8341 ggcgggcggc tccctcattg atgggataat agcggacagc cgtttgcggg ggcgcagcct
     8401 ccgggggttc ctcctgtgtc tggccggaag caactgccac atcctcaaaa gatagctgtt
     8461 cgggttcctc cggctgcggt tcgggcggcg tattgttgat aatgccgtca atcatgtttt
     8521 cgttctgctc cgtagacagt tcggcggtac gcaggggatt ttcccgcaaa aactccggca
     8581 cttccatata gccgaagctg tctacatagt gggcggtgtc ctgtccattc tggtgcagca
     8641 ccaccacgtc ggaaacggaa aggctgtgtc ccctaaaatc tgccgggtgg tcgatgttga
     8701 atgtgcggta aatatcctcc aaagacgtgc ttttatccag cggtgccgta taaacaaggt
     8761 catagttggc actgtccacg gccagccctg cggcctgtaa acgctcgtat ggctcaaacc
     8821 gatagtcgcg ggtttcctcc ccgcctcgaa gctggtagat ggaaaaggtg tcctgttggg
     8881 gtgcttcata gccagccgcc gcgtattcct ccacggtcat accggctgcg gcggcctcct
     8941 gcgcgatctc ggcctgtacc tgtgctttgt agtcctgcaa gctctggtcg aagtcagcca
     9001 gtttcggcgg ctccggcaac tgatactggc cctgattgag catggggcgg cactcctgca
     9061 cataagagag aagcgccccc aaataggccg tttcctccgg cgtcagggtt tccccggact
     9121 gtaagcggcc ctgcgccgtc tgttccattt gggcaaggct gcaatgcagc ttcatatagg
     9181 ggacaaactc gcctaacagc atatcccgat gggccttgtc ctgctcccag ccctctatgc
     9241 cggttgtagc gattacatgc tttttccagt tttcgtcctg cgcgtaaaat tggtggtagc
     9301 tctggatatg ttcgatcagg gaaccgtccc tatctccaaa atcctgccgc ccctcgtagc
     9361 tgtccggctc gccctgaaag atacagtcaa tgcggaacgc ggttttgtca tagcctgcct
     9421 ctgccggata tgctgcatcc agacgggcaa acagcgcgtc ggcctcatgc aacggcattt
     9481 gctggccgtc ctttagctgg gggctttcgc tccatagcac agtcacaagc ggctcggctg
     9541 ccggatcaag ggccggggcc tgttcttcct cggcctgcgc cggggccgct gcctccgggg
     9601 taacgtcaac cgtgacggcc tcctgttcct gccgggcctc gcgctcctgc tgaagctggg
     9661 caaaatggcc gtcgatctcg gttatcaatt cgctggccgt ggcccggatg gtttcaaggg
     9721 aggctttcag ctcggataac tccctgccgc tgctccaatc ggccacatag ccaaaagaat
     9781 agtccgacgt gtccagcccg taatactggc agacggtata ggccacgctt tcggcctgta
     9841 cctcgcgggt gctgcggtct ggccgggccg cctgttcctc ggcgggggcc tcccggtcaa
     9901 tggcgtggag cttggcatgg gcaatctcat ggatggcggt ctttagggtc tgcaattcgc
     9961 tcatgccctc gttgatggca atgcgctgtt ttgcgtagaa gcagcggccc ttggccccgt
    10021 ctgaaatcgg ctcaatgctt atgggatagg gtgatgtccg ttccagcgcg gcaaagaaat
    10081 cctgatactg ctgcacgtcg ccggtaagtt cactgatata gggcatggga aaatccttgc
    10141 cctcggtctg gctgatgtca aagactgtca ccaccttaaa ggccggaacc gtcacttcct
    10201 gttcttcggt cacgggcttg ccgtccgcgc cgatcacggg ctgctgggtg gccgggtcga
    10261 ttttctccat ctgctgctta accttgaacg gggccggggc cagtatctta atgcctttct
    10321 cgccccgctt cacatggcgg tcaaattcat cctgccacct gttatagccc gccacaatcg
    10381 aaccgccctg catggcgatc agcagcgtat tgttcagact gtaatcgtgc atcttggaca
    10441 tagcggataa gtaagccata tagcggctac tttcaaaaag ctcctgtacg ccggtttcca
    10501 gacggtcagt aatcactttc atgcgttctt tgctgttcat gccgtccagc atgatcggga
    10561 taaccggggg cggcgctttg tcggggcttt cggatgcctc cggctgctgg gcctgcggtt
    10621 cctcccggtc agcttccggc tgggggtagt gcatcacccg gtattcctcc ggcaccggct
    10681 ggccctcatg gacgcgctgc cattcgtcgc cgcttctcac cagatagccg tactgggtaa
    10741 aaacgccttg ttcctgctgg gcaatgtggc gtccaaactg cgccgtatca atcccggctt
    10801 tccattcctc cggcatatcc accatgccgg aacggttcag gtagtagtcg cccaactgtc
    10861 catcccccct aacgtcggga agatgcacat agtaatccac attttcggtg tagtcgatga
    10921 tctggccgat attctcaaag ccgccacggg gattttgcag ggcggcgttc agctctgcga
    10981 tctcggcggc gtcaagctgg cccagccggg cggcaaggaa attcagctcg tccacgtcgg
    11041 cggccagcac catgtcatag ggaatggcaa ggtgtctgtc ctccgggcag gaatacccgt
    11101 tgataaaaaa atcctgcgga ttgtcggcag taatgccaac atcccgcagg gcggcttgca
    11161 gcgtttcggt tgtggtgggc aaatccagcc agacgccttg tttgccctgt tccaaaaact
    11221 gccggttatg gacttggata gaaaatactt cgttcacgcg gtcactcctt tctctgttct
    11281 gtttcaaaaa atagggggtt ggggacttcc ccaacaagca aaaatccccg gcccggcagg
    11341 gacgcggccg ggggatttta ccgggagtgt agccacactc cctatgcttg ctgtgttact
    11401 gtcctttagc tcctgtgttc aatgcggtag ttgacgtact tgcccccggt atcagccaaa
    11461 agcaccgtcc cgtcgtaggt tttgccggtc tttacggaat agaggccctt taccttgact
    11521 ttgccggttt tgagcagcgc agcggcgatc ttcggggtga acgctttctt gcgttcctca
    11581 aaaaatcggt cattcttcca catgacgaac tggcaggccc ggtttccgca gtagtagttt
    11641 ttcttgccct catagacagc ctcaccacag cggggacacg tcccgatagg aacacgctcg
    11701 gcctgaaaca atttctgctt ttcctcactg atacaggaat aggtctgcac cagctcccgc
    11761 gcctgcgtct cgatgccgtc cataaattcg tcaggatcgg cctgcccctt ggcgatctcg
    11821 gtcagccggt tttcccattc ggcagtaagc tggggagaag taagcgcctc cggcagcacc
    11881 acagccagat tgatgccgtc cttggtggga atgagctgct tgccttttct ctggacaaag
    11941 cccatctgca ccagcttttc cagaatggcg gcgcgggtgg ccggtgtgcc taatcccttg
    12001 cgctcggcat cctccggcat atcctcggcc ccggcccgtt ccatagcgga caaaagcgta
    12061 tcttcggtgt agggcttggg cggcgtggtg aaatgctcgg tgacagaggc ggccacgtct
    12121 gcaaaggtct ggccctcgca aagctccggc agctcccgca ccgcctcggc gtcctcgtcg
    12181 gcgtcggtct ttaaggaaga acggaaccgg cgttcaatct ctttccagcc gggggaaagg
    12241 acggtcttgc ccttggcggt aaactcctgc ccggcacagg taaatgtggc ggtgacggcc
    12301 tcatagatat gcggcccggc cacggcacat aagagcttgc agcagatcag ggacagcagc
    12361 tttttttcac tctcggccag agtgccaaag ccctgcttca caaattcagc ggtggggata
    12421 atggcgtggt ggtcggacac ctttttgctg ttcaggatgc ggtcaaactg cggggaaagg
    12481 ttcaggccct tggcaaacgg caccagctcc cacaggccgg acacaaggct ttccacggcg
    12541 ggcagcatat cgtcggtaag atattgacta tccgttctcg gataggtcag cagcttcttt
    12601 agatacagtg cctgggcata gtcaagggtc tgcttggcgg tatagccgaa aagccggtta
    12661 gcctcccgct gcaaggtggt aaggtcatag agcttgggcg gctgttccgt ctttttctcc
    12721 cgcgtcacgg aaacgcaaac ggcctgcgct tttgcacaag ccgccttgac ggtgtttgcc
    12781 tcggcagcgt cggaaaagcg gctgcttacc gcgtccgcgc cgcccgccgt gatatggacg
    12841 atgtgatatt tctctttctt gaaatcggta atcttggcgt ccctgtccac cagcattttt
    12901 aaggtcggcg tctggacgcg gcccacagtc aggttcttgt ggtacaggac ggaaaaaagc
    12961 cgggtggcgt taatgccgat cagccagtcg gccttggccc ggcacagggc cgattgatac
    13021 agcggatcat agtcgccgcc cggtttcagg tgggcaaagc cgtccttaat agcactgtcc
    13081 tccatagagg aaatccaaag gcgctcaaag ggcttttggc atcccgccat ctggtagacg
    13141 aaacggaaaa tcaattcccc ctcgcgcccg gcgtcggtgg cgcacacaag gccggtcacg
    13201 tcggggcgct gcatcagcga acagagaacg tcaaactgct gtttcttgtc gtcggggacg
    13261 atgtactgcc atgtttccgg gagaatgggc aaatcctcat agcgccattt tttgaaacgc
    13321 tcgtcatagc gtccggcgtc ggcaaaggac accagatgtc cgatgcacca gctcaccaga
    13381 tagccgttcc cctccaaata gccgtcgtgt ttctccgtgg ccccgatcac gcccgcaatg
    13441 ctgcgggcca cgctgggttt ttccgcgatt acaagtttat atgtcattcc tcgtcctcct
    13501 gttcactcac aaaatcagct tccgggtcaa actcggtttc gggttcctcg tcctccaaat
    13561 cctcgtcctc cccaaaatca tactcgccca aatcgtcgct gccggtggtt tcagccttgg
    13621 gctttttgaa cttgaaatag taaagggccg caccgccgcc cgccagcgcc accagcagga
    13681 acacgatcag gccgcccgcg ccgcccttgg gttcttcctc cggggcttcg ggttcctccg
    13741 gctggggttc ctgcggctcc gggccgctgc actcggtcag gttggtttta cataccgggc
    13801 aattcgtgtt cacagccccg gccttgcatt tcgtggtaca ggagcagaca gcagcggggg
    13861 tgacggtttc cccgtcctcc atcagcgtca gcagatcggc ttcatccacc tgatttaaga
    13921 aatggacagt ttcttttccc tcgtcgtccc ggtcaataat cagataaaac acattgccgt
    13981 ttttggtttc cacggtgata aactgcttgc catttttcgc ggcgctgccg atgtcgtcaa
    14041 tcagtgacag gttcccgtcc ggggacaggg cggcgctggc cccgccgcca ttgaacagtt
    14101 ccagcagaga agcaatttcc tccgggtcaa tggagaggga aaaaccgccc tcgccggtag
    14161 ccgctacttc cttttcgctg acggtcatat agtcggaatg gacatagccg atgcgctccg
    14221 gcaagagaat tttaatccag tcgccgtctg tgccgactac ctcaaccgtc gtcccattgg
    14281 gaagctgggt gaacgcggtc ttgtcaagcc ccgcgccggt gcggacattc agcctgccgc
    14341 cgttggtggt gacggtgccg gtctgttcag gttctttttt gtcggtgccg gtggtgatct
    14401 ggatgccgtc gtccgtggcg gcaatgctca ccttggaacc gaacagcgcg gaaaacaggt
    14461 cggtgatgtc cttgccggtc agagggccgg tatctttttc ggcctcggtt tctgtcccgt
    14521 ccgtggcggt gccggtttcg tctgtttcag gctccaaacc gcccgtagcg ggttcggttg
    14581 tagcctccgg cagcggggcc ggtgccgggt cattgccgcc tgcaagggcg gtaacgggaa
    14641 aggccgtgac gcagagcatc acggccagaa gtgcggggag aatacgggaa atccgtctgg
    14701 tcttattctt catgggcatt gtcctcctgt tcatggtaag gggttgcggc aggggcggcc
    14761 tgcattgcgc cgctgcgtaa gaacgcggca aattcctccg gcttcatgtt catgccgcgc
    14821 acaagctgga tgatctgcaa attctcctgt tcggttttct gcgtttccag ctcccgaagc
    14881 tgtttctgaa actccgcgat cttagacttg gttttctgga tgtccttttc gatgcggtca
    14941 agtttggttg ccataattga aagctccttt ctggcgttaa ggtaaacgcc cgtaactgta
    15001 aaaatgggat tgccagtagt ttgaagtaag gttcgtgtag gaaatcgggt tcccgcaatg
    15061 cagcatcaca ttgttgccca catacaggcc gacgtgggac acgccggggg tgtcgtaggt
    15121 tcccttgaaa aatacaaggt cgccgggctt ggcctgcgaa ctggaaacag gggtgcagat
    15181 gttatatagg ccctgcgccc ccagccgtcc cacgttccat ccactatgat tgattaccca
    15241 cgaaatgaag ccgctgcaat caaagctggt gctgggagag ctgccgcccc acacataggg
    15301 ataaccgata tatttttccg cctctttgag catggcggca aaggtggcat cctccaaagc
    15361 ctccgggggt acgtcgtagt aggtcggcgg cgtcagggtg gaggcgttgg ggtactggct
    15421 gccggaaaac agatcaggcc ggtttcctag cgtggacata tacagggaat agaaacttag
    15481 cgtttcctcg tccatgatat agactggcac atgggacagg tcgaagtttt ccagcgtgac
    15541 gtagcagatg taatagtcat acggcacttg gtaggtgtct gtatgggtgt tgccctccgc
    15601 gtccgtccat gtatcggttt cggtgcggta acgggtttct accacgacgt tttctgtcag
    15661 gatatattgc ttgtcaaaga gcatggacaa atcaccctgt acctccccga tggtaaatat
    15721 cccgtcatgg agcgccgaca gaatggaaat cagcacatag gggtcatggc tgatctcgtc
    15781 aaggtcaaaa tggtattcgt catagtcatg ggtggcctcg tagttgtcca gatagtcttg
    15841 cagctcggct tccagctccg cgtaggcgtc ctcggcggcc aacatatcgc tatcctcgga
    15901 aaagtaagag gacgccgtga tgccgcttcc ggcgctgccg aacatggcgg tgcaggactg
    15961 taagccgccg atgataagca gcatcaaaag ccccaacaca aggacggcca gcgccccttt
    16021 ccagtggcgg gccacaaagg aggccgcccg ttcactttct ttggctgcgg ccttggccgc
    16081 tttcgtggag gccccggctg tcttggccgc gccctgtgcc gccgcgcctg cggtctggcc
    16141 cgctgtccgg gcggccttgg cgtactggcg cttgatctgc tgtttctgcc agaaccggga
    16201 aatggggttg ctggccgcct gtgcgatctc cgggttatcc cgcaaagatt tctggtagaa
    16261 atactccgcg ttggcagaca atagcctgcg ctcggccttt tccgcgtccc agtagggttt
    16321 cagcctgtgg tgccggatgg tgctgcggat ggcttttccg gcctgccgct cggcaagttc
    16381 ctccccctta tggccgccct ccacacccac gttttcatgc tcgacttcat ggattttccc
    16441 gtggccgaat agaaaaacct cggaaacagg acggcctgcc ggattgggtt tcagggaggg
    16501 aggccccttg tcctgtttgt caaagtgcag gacggtcttt gctttgccgc ttgcctcgtc
    16561 aaagacggtt tccttgaccg ggacgcgctt cttggggatg gcggcgcggg ccttatccag
    16621 cttatcggcc cgtttgtccg attttcggat atagggctgt aaatccgggt cggcccgttc
    16681 ttcctccgac agttggagcc gggcggatgg ccggtgcagg gcagcgtccc cctcggcggc
    16741 cacagccccg gcctgcttcg cggctttctt ggccttatgg ccgtcatgga tggcgtcggc
    16801 ccgttccagc agcttatcgg cggtgccgcc cggccccggc gcggacaaat cctgttcggc
    16861 ctcccggctg gaaatgttct ccacctcgcc ggtggcctga ttttccagcg tcagcccgtc
    16921 gcgggtcatg gcctgcgtca ctttcgcctt ggctttcagc tctttcatgg tggttcacct
    16981 cctttgtggc gtcgccgggt catactcccg ccacctcgtt taagcgggtc gtcataatct
    17041 tgtaaagctg ggtgttgtgc gggatggggt tggtaaaggg cagcaccacg ttttcaaaga
    17101 tcaaaaggcc ctcgccgggt tcggaattgt cgatgtactt ctgctgttcg gtggacaaat
    17161 tcaagcgttc cgcaaggatt tttctatccc cggacgcctg attgagcagc gtaatgaagt
    17221 cgctgttttc aaggatattt tcaatttccg gggaggaaag caggtctttg acgttctggg
    17281 tggcacctgt tggaatgccg ccccatttcc taaaccgctt ccaaatctcc gcagaatagg
    17341 ccgccgtctg ttcctctttc aaaagcaaat ggaactcgtc ggcaaaatac catgtggcct
    17401 ttccttggct tcggttggcc gtgacgcgcc cccatatctg gtcttggaca atgagcatcc
    17461 ccagcttttt gagctgcttg cccagctcct taatgtcaaa ggacacaagc cggttgccga
    17521 tgtccacgtt ggtacggtgg ttaaatacgt tcagggagcc ggacacatac aggtcaaggg
    17581 cctgcgccac ccggtcagcc tccgggacat gctgatctaa caacgctttg tggaggtcgg
    17641 acaggatcgg catattctcc gggcgggggt ctgccagata gggccggtag atcacgcgca
    17701 cagcccggtc tatcacggtc ttttcaatgg cttccagccc gttcttgccg cccatgacaa
    17761 gctcacagaa cgacagcaca aagtcggatt tcagggccag cgggttatcg tcctccgaat
    17821 aattcaggtt gatgtccagc gggttcacaa agtccttgct ggtaggcgac agccggatta
    17881 cctgtccgtg gagccgtttg acaagggggt aatattcggc ctccgggtct gaaataaaaa
    17941 tatcgtcagg cgtcgttaag aacacactga cgatctcgga tttacagctc atggatttgc
    18001 cgctgccggg ggtgccgagc ttcaaggcat tggggcaacg ggcctgcttc ctgtccagca
    18061 taatcatgtt gcgggacttg gcgttgatgc cgtaatacat ggccgcgccg ccctgaaaaa
    18121 gctcctgcgt cacaaagggg acgaacacgg ccacgctgga agtcgtaagg ctgcgctgta
    18181 tcttgatctg attgacgccc agcggcaggc tgcttgcaag gccctgttcc tgctggtagt
    18241 ccagccggac aaggggacag ttatgctttt gggccacgcc tgcggcttgg aacacgtcgt
    18301 tattcagctt ttgcttggtg tcggccacgt tgaggacaag gaacgtcaac tggaacagcc
    18361 gttcattgcg ggtctgcaac tttgtcagca gcttcttggc gtcctcgccg taggtggcaa
    18421 ggtcgctggg aagaatgtcc atatcgtaac cgctgcggac ggcctttttc tgctccgcaa
    18481 tcttcatagc gtcaaggtcg gtaattttgc gcttgatggt cttgatcgcc ttggtctgtt
    18541 caatggcctg cacatggaga ttgactaaaa caccgctttc cgtattgagg aagtcggcca
    18601 gcatatcgtc cgacagctcc ggggacagga tttgcaggaa acttaccgcg ccgtatttgc
    18661 cgcccatgcc gaatgtcttt gcattgccga agcacaggga ggacggggca ataaaatcct
    18721 tggtggagag gccggacggg gccagccact tccagtcaaa attgaatatc tcgccgtccg
    18781 ggtggtacac gccatgcagc aggttcagcc gttcccagcc gtccatcaca tgggctacgg
    18841 cccccatgct cttgaaatag cctaacaggt caaagccgat gcgcttcaat ctggctcgcg
    18901 ccgctttcac gctttcggcc tcgatggtga atgtcaggta tttggtcttt accatgccgt
    18961 tgttgccgtt tgcaagctgc ttttgcagaa tgccggtgta ctcggcccgg atgtcgtcga
    19021 agtcgtcccc ctgcggcgcg atctcaaagc tcttggcgta ctgcaccggg tcaactttcc
    19081 gattgaggaa agaaaactgg atatgaatgg aggcgtccac atagttatac aggtcgcaca
    19141 gtttttcaaa gatagccgtc tgcgtgtccg gctgggcaag ctgatagctg atgtccgcaa
    19201 actcaatgca cttggaaaag acgccgggcg atacccggca cacgccgtcc gggtacatga
    19261 cctcgtaggg gatgctctgc tgcgccgtgt ggggcctgcc gtcgccctta tatttacgga
    19321 ttaccgcgtc aatttctttt cgctgggcgc gggtcagctt tgttttttcg gtttctcttg
    19381 ggatttcctt tttggacaat ggcctttacc tcctgttcca tctggtattg ccgcacaagg
    19441 gcggtgtagg tgttgtcggt ctgataggga cgctcctttg gctggatgaa gcagcactgg
    19501 atgatctgca taatcaccac ttcaaggggc tggccgtgac gctcgtacat ggcaagcaaa
    19561 aagcccggca gcatggcaaa taccatgagc agggccgccg cgctgttggt aagatagtca
    19621 cgggtgagaa aatataaggg gacgccgatc accagcgccc cggaaaaaca gatcagttgc
    19681 cgtttggtaa gcccaaacac aaccttgctt ttcacatggg ttaagtcctt ggggacggtt
    19741 acataagcca tttggcacct cctttcacgt caggataagg tcacattcca cctcgttccc
    19801 ccacacgtcc cagccggggg ttttctgtct ggcaaacagc tccacgcggg gcaaatcgcc
    19861 cgccagctct acgatttttt cacgggtcac atccggcttt ttgctgtgtt gttcaatcgg
    19921 gctgataata aactgatgga cagaggcaga acgacgcttc ggcttgccgt gtgttgccag
    19981 caggcagatt tccgcgtttc cccttgtcca gcggcccagc ccgtaaaacc atgtaaggct
    20041 tttacggttc tgtttcagcc atacaaaagc cacggtcttg aatgaaaagc cccacgcttt
    20101 gattaaccgc agagcttccg gcagcatggg gaatgtggcc cacaaaaaca gcaggcagtc
    20161 tttggacgcc agccgttcca ccggcagggc gcacagctcg tcaatgctca tggtggaata
    20221 gtgattttcc gctgcgccct gtacgttttt gcagtcatac cgccacggcg ggtcagcgta
    20281 aatgatgttg tacgttgaaa taagcaagtt cctcctgtgt atgcttggat tttgggatca
    20341 atgcgccccg aatacggcct ttgatatact gccggtcttg aacaggcaga agcacagcag
    20401 caccgtatag cccatacagc cccagattgc gccggatatg tcggaagcgg aaccgatatt
    20461 ctgtatcagc accgcgtaga tacccaccac aaccatgatt aaaaaggctt ggaagcccag
    20521 cgccagcagg gatttcagat acccttggcc catgccgcgc cattccgcgt tgcccattgt
    20581 ggcaaggggg ataggcccca gcgacgtgac aacatagatt tccaccatgc ggccataggt
    20641 cacaaggaaa atgcagatgg aaaggatatt catggtgagg cccacaacaa gggtttggaa
    20701 ccacagcccc aaaagagcgc ccacgctcat agcctccaat tggctttcaa ggtctggtat
    20761 cagggtgtta aaatcaatgg atgtctgccc gataatcaca ccggcgcttt gattgacgac
    20821 gctttgggtg gcgtcgaata cgcccatgac gatgttccat gtgttcgtta cgatcaggat
    20881 tgcaaacgcg gatttgaata tccagcggaa gaacatggag ctgtcaaagt cgtgcatatt
    20941 gttcttttcc gttatcatct ggataagctc atagcacatg acaagggcga gtatggcccc
    21001 ggctatgggg acgatcacgg tttccgacag gctgtgcagc atattgaaaa taccgctgtt
    21061 ccatgcctgc ggggttcgtc ccacgtcggc ggcgatttct ccgactttgg catttaccgt
    21121 atcgaacagc ccggacaggt tgcccgtgat accgtccatg aggatgcctt ttatccattc
    21181 ttcaatgatt tcactaatca cgggtagcac ctcctttccc gcgccctccc ggtctggccg
    21241 ggaggacgcg gaggcgatta gttaagggtt tggacaggga caaaggccag gatcagccca
    21301 gcagcccgga cagcagggga accagagtga caccgatcag ggccacgccg ccgccagcga
    21361 caagctgctt gacaccctgc gattttgccc ccggattgtc ctgcccgtat ccctccaaca
    21421 gattgatgcc gccccatacg cacagagcgc cgcccagtcc gatcacgata gtctgcaaag
    21481 tagtaactgc ggaattaaaa aatgccataa atttacctct ttctgccggt ttccggctca
    21541 taatgatttt ttgattttgt tgttggaatg aaaaaagccg ctgtttattc agcggcggcg
    21601 tcctcgtccg acaggtcgat ttcatacagg tcgaatactt catcctgcgt taccacttgc
    21661 tgccgtttcc ggcgcgtgat agtcaaaaat ctgtccacgt caaaggtatt cttcttgtcg
    21721 gcgtcggcaa ggtatttgta ccggggatgc ttggtaatat cgaatttctc cgataaaaac
    21781 gggcgcacac cccgtagctg caagatacac ttgccgccgt ccatcacggc aatttcgtct
    21841 tgggacatca actcctttcc taatttttga tagttcaatc cgtgggacgt ttggcttccc
    21901 cggttctcgc tggtgttgaa actgtcaatc gtttctttcc ccagcgcatc ggaaatctcc
    21961 ttggcatttt tgccgcgccc actaaggaaa agcacactat cgcagttatc cgaaatgatc
    22021 tcggcggcgt ccttgtaaat cgcttttagc tgcgactggc tctgcaaaat aatagaggcc
    22081 gatatttcac ggcttcgaat ggtggcaatg agtttatcga aattgggtat ttggccaatg
    22141 ttggcaaatt catccaaaat caggcgtaca tggacaggca gccgcccgcc gtacacatca
    22201 tcagcccggt cacaaagcag gttgattagc tggctttgga gcatagccag aatgaaatta
    22261 aaggtcgtgt ccgtatcgct cataatgaga tacagcaccg tcttttcgtc cccgatggtg
    22321 tccagctcca tttcgtcgtc ctccatcagc tcgcgtacct cccggatgtc aaagggcgca
    22381 agtctggcac cgcatgaaat caggatggaa gaacgggtct tgcctgcgga cagcaggaat
    22441 ttcttgtact ggcgcaccgc aaaatggtcg gggtctttct gctccaaacg ctcaaacatg
    22501 agatcgacgg ggctttgaaa ctccgggtcg tcctcgcggg cttcactggc gtttatcatt
    22561 tccagcagcg ttgagaagtt catttcctcc gctggggcct cgtaccagat atagccgatc
    22621 agggccgaat aaaacaaccg ttccgacttc acccaaaaat cctccgctga cttttccccc
    22681 tcgcccttgg tgttggaaat cagggtattt accagcttta gcacgtcctt ttccgaatgg
    22741 atgtagacaa aggggttgta cttcatggac ttggaaaagt taatggtatt caggatttta
    22801 atgcggtagc ccgcctgctg gaacatcctg ccggtttcca gaaccaaagt gcctttcgga
    22861 tctgtcacca caaacgaagt cggatagtcc gtggtcggaa agcattgcat caggttgggt
    22921 ttcacgaaaa atctcgtttt ccctgaaccg ctgccaccga tcaccagcac gtttttattc
    22981 cgcgctgtct tggggtcttt gggcctgccg gtcatggtaa gccgttccgt ttgggtgagc
    23041 aggatattat tttcaaactt ggggtcgatg taaggcgcta tgtctttggc cgtcccccag
    23101 cgggcgctgc catactccat gcctttgcgg tatttcttca cgttcttgct cttgcagtac
    23161 accaccagcc gcacgatcac ggcaccggcg ataccgatac acaggtcaaa caggtgcagg
    23221 cttggcagcg ggttggcaaa agccgccgca aaaccatccg caaaatgcag gagcttcccc
    23281 gacaggtcag caccagcggc cagccggtac gcctccccgc atttcgcaaa caggtacaca
    23341 aaaatcagat acgggaggtt caggatcagc aatttcttta cgtcaggctt cacagttcca
    23401 gccccctttc cttgattttc tccttggccc gctgcttggt ctgggccgcc gccttttgct
    23461 tcacagcgga caaaaccttg cggatagagg gccgctgctc cttggaaagc gtcttggcgg
    23521 taaactcccg aaacgccaga tcaaggttat ctgcatcctt ggccttgaaa atcacaacgt
    23581 agtggggcgg ctgggtggtc ttatcttttc gtaaggtaaa atccacgccg tatttcttgg
    23641 cgcatggctt gaacgctcca atgttggcct cggtgatctc aatgttggag agatcagccc
    23701 catgcttttt aagctgcttt aggctctgct ggccgtggtg cagcgtgttt ttgcccttgg
    23761 cccggtgttg caggtacttt ttcagcgccg cttgcagcac ttgagccgtg agcttgcccg
    23821 ttttcatgga cagggcaatg gtcttttgag taacttcttc ctgcaatcgc atccctcctt
    23881 gtcagaaagt caaaagcggc gtctaaggcg gaaccaccag acgccgcagg tagatatagg
    23941 tcaaatccgc acccgcaggc cggaaaggtc gtcggcccgg atgccgacaa gataccagtt
    24001 ttcggccatt tcaatgcggg tgggcctcca tttgccgccg atcatcacgt cgaaacactc
    24061 cccgcaatgc aggccgccgt aatagtcggc cagatcaaag cggatgtcgt aacggtctgt
    24121 tcgctcgtca aaaatcaatg cgccctgctt catagggtcg tcctcctttt ctcgttacag
    24181 gctttcgccc tcgccgcagg aatagtccgg caggccggat ttaggctttc cccaatcggg
    24241 ctgtgccatg tcgtgcgcga caagggccgt gtagtagttg gaaatggtgc tgggggcgtt
    24301 gaacagcata gcccgcaggt attgtttgat attgcggact ttcgttgtgt tctcccgcag
    24361 acagtcaagg acaaagcgga tatgttcgcc ggtcagcttc atgtacttgg atttcaccag
    24421 ctcggcaggg tagtcgtccc cggcgacacg gattgtcttt cgggcggtgc agacggtttc
    24481 cagcatcagg tctacgattt cgtcaagctg ttcacggtca atgctcctgt cctgaatgag
    24541 ataggcatac tcgatgttct ccaaaatgat ttcccgataa atccgtacag cctcatcctt
    24601 tcctgtcctt ttccgttccg gcggcgtagc cgctgcctcg ccctccatag gggcaggggt
    24661 cggggatggg ataggaatgg aatgggtact ttgtccatct gtaattattt tttctttttt
    24721 tgataagtca gttctttgta cttctttatt taattgcgtt ggattttccg acgccggatt
    24781 atccgatgtc ggattttcca acaccgggtt atccgatgtt ggattttcca ataccgggtt
    24841 ttccaatacc ggctgttcgt agatggtata gaccatatcg gccattttgc cttttgcgtc
    24901 acggccctgc tcacgccgga tatatccagc tttttcaagc tcccataccg ctgtcctgat
    24961 cgcgtcgatg ctttcccggt tgatatagga cagtcctttt agggtatagt cccaatcctc
    25021 cggcaaggac aacatctgcg acagcaggcc cttggccttt aaggtcaggt tcttattccg
    25081 caggtggtga tttgacatca ccgtatagcc tttgttcttt tccacacgga aaactgccat
    25141 agcgttgttc gctcctttca ctgtgaattt gaccgctgca tagtcggtca gcttgcccgg
    25201 ctgataggga cactctgcaa actggcagaa ttgatatttc caatccgggc ggtaaaatcg
    25261 gcaggtgccg caatcctccg gcgcgtccgc cccgccgccg tcgtagtgat cgaaaccggg
    25321 cttttcctgc atcagccgtt caaacgcccg gtcagggccg gaagtaaata gcatggcgtc
    25381 gcctcctttc tctatttggg cataaaaaag cggcgtcctg ttctcccatg acgggaaaga
    25441 ggaacgccgc gtttacggtg tcgtattcaa tttatgatgt gccgtatttg gggtggcggg
    25501 tattagcagt atggttcagc atatatgcgg ctcaatccga aagtagtaaa ttggtagtaa
    25561 attgagttct catatcctgc gaaatgcccg tatttccggg gctttcagcg ataacctaaa
    25621 tttttggaat aaaactttca ttataggcta ctgcaacatc catccaatca gcaatctctt
    25681 tttggatctc aggtcgtttc cagtagccaa cgacgatttt attttgttta tttaatcgag
    25741 cattgatgaa cccatattcg cggtcaccat gagcggattg atttaaattc atgaaatcca
    25801 tatcgatcgt tttccaagga atgctttcgt tatattgcgt agctaaatga agcaatggtt
    25861 tttgtagaag ctttgtcccg cggatccaca tttttgctgg tgagaaagta tgcatccaag
    25921 tgatcacacc tgctacttct tctcgataat tgacctcttt catgattttt gtgatcgtat
    25981 cagcagtaac tgctaattct tgaaggcgga ttggataagg caattggcca ttttcattca
    26041 gttcatcgac catttcttgt gcatgtttct ttacttctcg cagtgcttct tccccatata
    26101 aatgctgtga tcctacaaca aaccaaaatt ctttttctcc aatattcaac ataattagcc
    26161 actcctttta tttgttttgt ccgtaatacg cattttttcc gtgttttcgt aaataatgtt
    26221 tatctaaaat ccgttgaggc agctcttctg aaaatgaatt gagctgtctg gtaaataaat
    26281 tcatcttgca tacttcatct agtacgactg cgttcattac cgcactttgc gcatctttcc
    26341 cccaagtaaa tggaccgtgt ccgtgtaaaa gtacccctgg aattgctaag ggatcaattt
    26401 tgcgttcttt gaatgtttct atgataactt ttcctgtttc atattcgtac ccgctgtcga
    26461 tttcctgttg tgtcaaaaat cgagcacaag gtaccgatcc ataaaaagta tctgcatgcg
    26521 tcgtccccat cgcaggaaca tccaatcctg cttgtgccca gatcgtcgcc catgtcgagt
    26581 gagtatggac gattcctccg atttgaggaa atgaacgata taaaacagca tgagtaggcg
    26641 tatccgaaga gggattgagt ttaccttcaa tgatatgatt gtccaagtcc acaacgacca
    26701 tgtcgtctgc tgtcatctct tcataactga ctccgctagg tttgataacg aaatatcgtg
    26761 tttcaggatc aaaagcgctc acatttcccc atgtatattt gacgagtccg tgtttaggca
    26821 agtcaagatt tgcttggtat acttcttctt tcagttgttc cagcattttc tatcactcct
    26881 ttttcttgca attgatgaat cgctgttttt tcaatgtcga tcccctcacg gtaacgtctc
    26941 ataaatatct gatagcctgc tatttcttct gctgaaggtg tgatggtgat tccttggtct
    27001 tgtccaaaca cgtcttctgc caagaagttt tctaaagact tcccatgtcg atccatcata
    27061 taggctgcta ataacgcgat tccccacgct ccaccttctc cggctgtgtc catcactgta
    27121 actggtgcct ccatagcaga tgctagaatc cgctgtccaa cttctggtgt tttaaaaatc
    27181 cccccatgcc ccactaaacg atcaatttgg acattctcag atttcaatat atccattccg
    27241 attctcattg caccaaaagc actggctaaa tggatccgca tgaaattcgc taaatcaaaa
    27301 cggctacctg gtgtacgtac aaataatggc cttccttcat ttactcctgt aatgttttca
    27361 ccggaaaaat aaccataaga aagtaatccc ccgcaatccg tatcgccttc tagcgctttg
    27421 ttgaataata ccgtgaataa tttatctctg cttatctcca tacccaaaga attggtaaat
    27481 tcttcaaaaa tactgaccca agcattgata tccgaagagc agttattcgt atgaaccatg
    27541 cctactagtt ctcctgaagg tgtagtgacc atgtcgattt ccggatggac attttctagt
    27601 tctttttcta atacgatcat agcgaaggcg gaagtaccag ccgaaatgtt ccctgtccgt
    27661 ttggctacac tgttcgtagc gaccataccg gttcctgcat ccccttctgg cggacaaacc
    27721 gggattcctg gtctcaaatt tcccgtaggg tcgatcagat aagcccctga ttcgcttaac
    27781 ttgccagctt cttctcctgc tactagaact ttaggcaaaa tgtgatccaa gtcaatcgct
    27841 acgtgcgctt tttcgattaa ctgaccaaaa atatttttca tttcttgatg gtaggttttc
    27901 gttgaaggat cgataggaaa catgcctgat gcatctccta cgcctagtac tttctcacca
    27961 gtaagctgcc aatggacata acctgcaagc gttgtcaaaa aattaatttc tttcacatgt
    28021 tcttctttat tcaaaataga ttgatacaaa tgagcgatac tccatctctg cggaatattg
    28081 tattgaaatt tttccgttaa tattcgttct gcttcttcag tgattgtatt tcgccaagta
    28141 cgaaatggta ctagtaactg ttccttgcta tcaaatgcta gataaccgtg catcattgca
    28201 ctgaacccaa tcgacccgat tgtcgtaaga ttctcgccat agatttcgaa tacttctgct
    28261 gccatttttc ggtaactgac gcgcagtcct ttccaaatat cttctaatga ataggtccag
    28321 ataccatttt ctagtttgtt ttcccactca taactaccag cggcaatagg agtaaaagat
    28381 gaatcaatca atacagcttt gatcctagtt gatccaaact caatccctaa agatgtacga
    28441 ccatctatga catctaacat actttgcgca tttacttcct tcataatgat ttaactccct
    28501 ccttctcgtc aaacctgatc aaaccgtctt tttagtagcg cttacaatga ttattgtata
    28561 cttttgtacg tacatgtcaa gcggttattt acaagaatac aataaaaaac acttgttttg
    28621 tacgtacata agttataaaa tgaaattgct tacaaaatct gatcaaccgc cttctcgaaa
    28681 ttctagaagg agagaatact tatgaaaaaa gaaaaaaaag tttcctcatc gtttatttat
    28741 ttttttggcg catttggcgg cattctattt ggatatgata ttggtgttat gaccggcgcc
    28801 ttaccttttt tacagcatga ttggggtttg gcaggtaaag cttctttgat aggatggatc
    28861 acgtcttcgg tcatgttagg tgcgatcctt ggcggctctt tatctggtct tctttctgat
    28921 aagcttggac gacgaaaaat gattcttctt tctgcactta tttttatggc tggttctgtc
    28981 ctttctgcat ctgcccctca caatggttct tattttctga ttgcagcaag gattcttcta
    29041 ggtttagctg ttggcgctgc atctgcccta gttcctgctt atatgtctga aatggctcct
    29101 gctagactaa ggggaagatt gtcagggatc aatcaagtga tgattgcttc tggcatgctc
    29161 ctttcctatg ttgcagatta tttgctaaaa gtgttgcctg aaacgatggc ttggcgcgtg
    29221 atgttagggc tcgctgctgt ccccgcgctg attttatttt tcggtgtttt agctctacca
    29281 gagtcaccgc gttttcttat gcaatctggc cgcttggaag aggcaaaaag agtattgaac
    29341 tatattcgga caccaaaaga agctgaacag gaattcgaac aaatccagct gaatgtcaaa
    29401 caagaaaaga caaccggaac ttcttggcat actttgtttt tagaaaaata tcgttcctta
    29461 gtcattgccg gtattggtgt agcagtattc cagcaatttc aaggagctaa tgctattttt
    29521 tactatattc cgctgatcgt agaaaaagcg actgggcatg cagcgagcga tgcattgatg
    29581 tggccgatca tccaaggaat cattttagtt gctggttctt tgctcttttt ggttattgca
    29641 gacaagttca atcggcgaac tttattgaaa ataggcggta gcgtgatggg actttcattc
    29701 atcctaccag ctgttttagg aactgtactg gatgctcata caaattcgtt gttgattctc
    29761 ttgtttttat gtatttatgt cgcattttat tcttgcacat gggctccact gacatgggta
    29821 atcataggcg aaatattccc actagctgtc cgcgggcgag catctggcct agcctcttct
    29881 tttaattgga ttggttcttt cctagttggc ttgctttttc ctgtgatgac tgcttctatg
    29941 tctcaagaaa tcgttttcgg catttttggg atcatctgct ttttaggggt tttatttatt
    30001 caagagatcg taccagaaac tcgtgggaaa tctttagagg aaatcgaaca aagtgcatct
    30061 aaaaaaactt atcctaaacg tatttctatc catcattctt aaagaggttg tgacagaagt
    30121 ggtcagcttc aagaaataag aaggaaccgc ttagtttcca gtcacaagga acaatttcaa
    30181 aaaacagctt ctcatgtttg atgaatattg tgacttgtga ccagttggta ggagaacacc
    30241 gtttagtgga gaggtggtga aagaaacgtt cagccttgag cgatgacaaa agctctcaca
    30301 aacattgcga cgagtaaccg taggagcaat aagaggcggt aaaaacccgt ctcttatttt
    30361 tcgttttctt tcactgtttc ccgttcaact agtacaggtt catagagaat atttggctgt
    30421 gcctgtcctt tttcaatcgt tgtgacgatc caccgtgcgg catcttgtcc caacttttct
    30481 tttggatgcg tcaatgttgt caatttttta ttccctaaat ggcttaaaga agaatcgtct
    30541 gttcctacga tcgataggtc atctggaata tgatagccat tttctgtcaa cttatctagc
    30601 attaaactag ctatttgatc gttatagcaa atgatgccat ctaaagcttg ttcttctttc
    30661 aaccgttgta ctgttttttc agcgattgtt tctcgtgttt ccgtggtgaa agtaatgata
    30721 tcttccgtca aaaaacgcac attatactgt tcacacgcgc tgataaaacc tttcatcctg
    30781 tattttcctt gaagatcgtc gatttttgtg atgaacaaca gacgctgatg tccttttttt
    30841 atcaaatatt ctgctgccat aaaaccgact ttcacatcat ctaaacaaat ataagaggca
    30901 tttaactctt cataagcagc attgatcatc accatcggaa tgccttgttc tctcaaagca
    30961 acataaagtg ctaagtttgg gttatactga ttacttttcg taggctcaac gatcaaacca
    31021 tctaccccat aagcaatcat tttcttcaaa cactctttct ccaattcgtg atcgttattc
    31081 gtactggcta aaagcaatga atacccagat tctcgtaatt cattttcgat tcctcggata
    31141 atcgacggaa aaatataatc agagagatat gtggtgatga caccgatcgt ttttgttctg
    31201 ttcaatggtg ttttttcttt cttatagctg tcatctacat atgtgcccga ccctttttct
    31261 ttacgcagat agccctcatt taccaacaca gcgattgctt gccgaatcgt atgtctgctg
    31321 acgtcatata tttcttgcag cttgaattct gagggaatgg ttgtatttac ttcgtattcc
    31381 tcacgaagga tcttttgttt tatctcttct gcaatatctt gatattttgc catttgcctt
    31441 gccccttttt atttgtacgt acatatatca taacgagtct gcaaggttta ttcaagcaaa
    31501 aatcaaggtt tcttgtgctc ataaggcata cggaacaggt tcaccaaatt ctggaaatcc
    31561 ttgctcattc cacgtaaagc attgcgcgtt cgcgtgccga ttaggattgt ctaacggatc
    31621 ccctttttgg ttcttctctg gtcgagcatg atagatcaaa atatcaaagc acccgtcttc
    31681 tgaaaccgta aacgagttgt gaccaggacc aaattgctta tttttttcag aagaaacgaa
    31741 tactggctcc gataatttat gccatgattc ttttaataaa agattcttgt gctcatcagc
    31801 ccacagcagg cccatggcat agttttcatc tgttgcacta ccagaatacg taatgaatat
    31861 tttcccattg cgagtaagga ctgctggacc ttcatttact gaaaaacccc gtttttccca
    31921 ttcatactca ggtatagaaa ggaggatctg cttccccttt aacgtccaag gattatccat
    31981 ttcggataga taaagattag aattcccagt aatttcagga tcttgttggg cccacacata
    32041 atatagcttt ccttgagaag agaaagttgt tccgtctaaa ctgaaagttt cccttttcgt
    32101 ttttatttgt cccttttcta tccaatctgt attcattgga tctatatgct gattttcaag
    32161 tacgaacatg cggtgatgat gagattcatc acgtatagtt ttatgattgc ttgcagcaaa
    32221 gtaaatatac cattttccat tgatataatg gacttcaggt gcccagatca attcactcat
    32281 cggtccttct tcatgagcct gccatacaat caggcttgca gcatgttcta attcattcag
    32341 tgactttgcc cttcttaatt caatcacttg gtagcctgga accgatcctg taaaataata
    32401 ataaccatct gtatgtttat atacccaagg atctgcccgt tgccgaacga taggattatt
    32461 ataaagtgtc ttcatcataa caaatttcct cacattttct ttattctaat tacattccaa
    32521 gagtattttt ctatccctac atttaattcg tcatgttttt ctattgaaaa atagtttatt
    32581 tcaggtttta ttttttctgg ttcttcacgt gtatttttgt ccaacagatc gctactggtt
    32641 aatgtgtagt gctctccttg atctagcaaa ttgaaatcat gttcaatcgt tatcttttgt
    32701 tgttcatttg tgcgattgat caagaaaata gtcagctgat tgccgttatc tacgatcaca
    32761 ctgtccacat aaggaacatt ttttatcttt tcacaatcat aaacagggca aatgatttgt
    32821 tgtttcaata cttgaccttt agcatatttc gataagtgtt gcatcacata ataagttgga
    32881 ttcctccatg cttgtccttt agcatctgtc agtattaaag gtattgtgtt tactaacaat
    32941 gattgacaac taatctttac gcgatctgcg tgtcgtaaaa tagccagccc taatgaacca
    33001 aataataatg tatcttccat agtaaagtaa caccaatcga tgggactacc gacttcaaaa
    33061 tccctatgtc ttttttccgg ttgggcatgt acattccatt catcaaacgc tagatacatt
    33121 gttttctcac ttctcaaata tccttttaca taatcacaag tagcaacgat ttcttcaatt
    33181 tgacgatcga atcgataagc ttttgcaagg tatgtcgctg tatcatcacg ctcattttta
    33241 ggaggtttag tcaagtcttc ttcatcatac ttatcaatat agttatgcaa agctaaataa
    33301 tctacattct cataagcttc ttctaatgcg atacgatccc attcagggta cgatggtaaa
    33361 cgtggcgtag agcttccaac taagattgtt tcaattgaat catccactaa cttcatgact
    33421 tttgaggctt catttgctaa cctaccatat tcataagcac tcttagcccc cacttgccat
    33481 ggaccgtcta gctcattccc taaacaccat aatttaacgc caaaaggctt tttttgccca
    33541 ttttttattc ttaaatcact ccagaaggtt ccttttggaa aattacaata ttctactaaa
    33601 ttttttgctt catcaattcc ttttgttcct aaattcaccg tgaatatcgg tttcgcttgt
    33661 acatgactga tccatttaaa aaattcgtgg agtccaaaag aatttggttc tatacctcgc
    33721 catgctaaat caattttttt tggtcgttgt tctttcctac ctacagtatc cacccaatca
    33781 taaccagatg taaagtttcc tccaggatag cgaatgacac ctaattctaa ttcctttaca
    33841 taagagagga catcttgtct aaacccgtca ccatcagctg tttcatggcc cggttcataa
    33901 atccctgtat agacgacact tcccatatgc tcaacaaatg agccaaacag ccgagaactg
    33961 atctctccta ccgtaaaatc ctctctgatt tttactttca cttcttcgtc caacagcttt
    34021 ccctaccttt catttcctat agtcaagata atagatacaa gtttccatac gccaccagca
    34081 aagataaaaa taaaaggagg gaaaagaaat atgagcagta caaatcctaa taacaatacg
    34141 caacaaagaa tatttctcaa aaaatcaaaa aatactttcc atgcactttg ttcaaaaatt
    34201 tgtaaaaatg tgatatcttt gttttcagaa atcacatata tccaggataa aagaaacatg
    34261 gaataaataa gtaaaactac atatccacta attgaaagta cgattgtctg ttcttctaat
    34321 aattgattaa caagaaaata aataatgatc gttaacagac taaggaacaa gttcttacca
    34381 aaatgttgct ttattccttg aaagaaaaaa taaatgatat ttcctgatcc ctcacctttc
    34441 cattgcgcca ttacataaaa aacagcacat gtagcaggca cgatagtaat aacaggcaaa
    34501 cacacgatta cccagagaat acctaataag atgtaatcag aaaccacaat caactgtcca
    34561 taaaatttag tctcaactat tttcatcttc ctcactcttt cattccagac atagctgttc
    34621 cttgaacaaa atatttctgt gcaaaaatat aaaccactag taatggcagc attgaaataa
    34681 ccgttccagc cattacaggt ccataaaaag aaatgttctg ccctgtaaat aaagctaacc
    34741 ctgctggtaa agttctcatt tttgaactac ttgtcaatat caatggataa agcaagtcat
    34801 tccaactatt catcagcgtg aaaatcccta atgataaaag agtaggtttg ctcaatggca
    34861 acatgatttt caaaaaaata ttaaactcac ttaatccatc aatgcgtgct gcctcttcca
    34921 aagaatctgg taaagtaata aaaaaggaac gcatcatgaa gataccaaat gcagttgtca
    34981 tacgaggaat gatcaaacct gcataagtat caagcatgcc aagcaaattg acttcaataa
    35041 atagaggaat catgaagact tggaatggga tcatcattgt cagcaaaacg aaatagaaca
    35101 aaactgattt tcctttgaaa cgcatacgag caaatgcata ccccgcaaga gaatcaaaga
    35161 atacagaagt gatcactgtc cctccagcaa aaatcactgt atttttgatg taatctaata
    35221 aagggatcgt tgtccaaacc ttaatatagt tatctagggt atagctatct gcaaatattc
    35281 taggcggaaa tgagataatg tctttttcgt gtttaaaaga agaaataatc agccacaaaa
    35341 aaggaaacgc aataaccaac aatataacta gcatactaat cgttcgaatg atttttttac
    35401 cactaactgc ttttcgacta ttcacagtaa ctgtaatcga gctttctggt gccatacagg
    35461 tttccctcct atttcatttg tttttctttc cgtgacatgt acacattagt ggagatagct
    35521 atagttgcaa caatgataaa caaaaccgaa gacagtgctg atgcatagcc caattcataa
    35581 ggtgctgtaa atcctcgact gtaaatatat tgaacagctg tttctgtctt aaattgtggg
    35641 ccaccagcgg ttgcaacata tacttggtca aacacttgca tcgaaccaat cagattcgtt
    35701 aaaatacaaa aacctaagga gggaacgatc tgaggtagcg taatgttaaa aaactgctgt
    35761 cgatttgttg caccatccat ttgagcagat tcatacaaag agggagagat tccctgaata
    35821 gcagtcaaga gaatcgtcat agtaacacca aaattcttcc aaacagtcat gatggctacc
    35881 gtcggcatag caagggttgg atcgcgaaaa aattgaggga tcggcaagcc gacttgtgaa
    35941 aacaagtaag gaatcgcccc tacattcgga tcaagcaaca tagaaaaaat aatcgcaatt
    36001 gacgtcaatg aacaaataac tggaatgtaa aatgtcgaac gacaaaaacg attgaatctg
    36061 gtatttttag ataatgctac tgctgctaat aaaccaacga taatttgtgt tggcgtttcg
    36121 agtacggtga aatataaaga atgaatcaac gaattaactg ctcgttcatc actaaatata
    36181 cgtgtgaaat tttcaaaact tacaaaattt cttgatgtaa agaaaatatt caaatctgta
    36241 aaactaatag caaatgttcc taaaagcggt atgacagtaa ataataataa aactaaaaaa
    36301 gatggagtta aaaacaaata cgcagcacga tcaggacgat tccaatattt ggtgaatccg
    36361 ttcatatagt ccacctcatc tttcattttg ataaaatata caatcttggt ttaaacgaat
    36421 ctgaataaga tatccgaaca atcgctgtga tttttgcaat aacacctatc tgcaaatact
    36481 aggtcgatca ttcagatatc tgtatcttgc caacagtcta tcctgtataa agttctgtac
    36541 tatttttgca gtaattgatt gatggcatca tctgcatcgt tcatgagtgt ctcaggatct
    36601 tctcctgcta ataatttttc aatcagtgga ttgatgatat cgttattgat cgcagagatt
    36661 tgggtaaaac ctggtaaata tggctctgca tagtccattt gtttcgataa ttcagataca
    36721 ataggatcat tttttacttc ttcatctttt gctactgaat ccaagtaggc aggaaaacca
    36781 ttttcgatac tccatttttt accaatttca gttgtattcc aatatttcaa aaactcatag
    36841 acagcttctt ttttattatt atctgtactt gccggaatgg catatcctac gccatctaaa
    36901 atagctgttt ttttgtctga atcactccct tgtggtaagg tagtcacatt ataatcaatc
    36961 tcgtttgctt tcaatccgtt atttaaccat ggcccattga tttcaatggc tagttgacca
    37021 gcattcatta aattgtccat ttcggcgcca gaaatcgatt ctggaccagt cttgtctaca
    37081 tggatcattt tttggatcgc ttttaacgtt ttaaggttct caggagacgc aaattcagac
    37141 ttagtataat catcgttcac taatttaccg ccatttgcta acatccagtt atataacggt
    37201 gcagttccat ccttattgaa gacaaaacct gatacatttt tactactatc agttaatttt
    37261 ggtgccattt ctgtcaattc ttcccaagta gtaggtggtt gttcagtatc taatccagct
    37321 tgtttaaaca aatcattatt ccaatacata tacatgcctt gtacctgcat ggggacaaaa
    37381 tactgtttac cttctacttc acctaattca atagccgttt ctttaaaatc agatttatct
    37441 actccatcat attcccagaa atcgtctaat ggttgtaccg ctccgttttc tacatactgt
    37501 gcaaaatctg gataatttaa tgcaataaag tcaggtgctt ttttagaaga aatcgctgta
    37561 ggaagttttt cattcaaatt ggcccaagtc atgatatcca tttcaatttt gatgttttta
    37621 tcatttgttt tattaaattc attaacgatt tcttgcaata tttcaccatc agatgcagta
    37681 aacccattcc aaaaagtcaa agtcacttct ttatcagaat cagattctgc tgaacttccg
    37741 ccaccgcaag ctcctaaaat acctgttgca gctaccaaca aagagccaat taaccatttt
    37801 ttacctttca ttttttgttc ctccttcatc tgataaacga catctgacca cattccaaga
    37861 tagcggtttc aataaacaat tgattttccc atcttcagct tttacatttg ttgaatgact
    37921 tggttttact tgttcacttt taggaagatt agttgctttt gtatcaaatc cactcatttc
    37981 agaaaaatca atcactccct ctaatgataa ttcagacaag tgaacagtta gattcatttc
    38041 ttcttctcca cggttaactg caaatatgac aagttctttg tcatttatta cggctatact
    38101 ttctaaatac ggcacttctt caaaatcttc cgtcgcatag tttgctgaac gaatttgtgg
    38161 gcttaacacc tctccttgtc catacacagc agcttgcata aatggataat agatcgtctg
    38221 tctccaagct tctccgtttg tttctgtcct gattggtgca ataacgttta ccaattgagc
    38281 aagacaagca atctttactc tatctgcatg tttcaataaa gtgattaata gacagcctaa
    38341 tagtaatgca tcttcaaaat tatagttatc ttctagtaaa ggaggagcaa tctgccaagg
    38401 cgctgcttgc tcatcttgtt ccgctgaatg ataccaaata ttccattcat caaaggacag
    38461 gttgatctgc ttcttactat gtttcttcgc cttgactgta tcgcacatcg ctgtcacttc
    38521 agaaatgaac cgatccatgt ctaaggaacg tgcaagataa tgagaaagat catttttttg
    38581 gttgccataa tattgatgca gagacaaata atctatttga tcgtacgctt gatcaagaac
    38641 tgttaactcc caatcaccaa atgtaggcat ttgccgattc gaacttccgc ataaaaccac
    38701 ctcaattgaa tcatctacga gtttcatcgc cttagccgtt tcctctgcta accgtccata
    38761 ttcttccgct gttttatgtc caatctgcca aggaccgtcc atttcattcc ctaaacacca
    38821 tgtcttaatt ccaaaagggc tctcttgacc attcgaacgc cttaaatcac tccaataagt
    38881 tcctttagga aaattacaat attcgactaa attacgagct gcatctactc cacgagtacc
    38941 cagattgaca gccatattga tttcggcccc cacatgctga caccagttat aaaattcatg
    39001 aatcccaact tcattcgttt caattgtccg ccaagctaaa tccaaccttc taggtctatt
    39061 tgctatgggc ccaatactgt cttcccaacg aaatcctgac acaaaatttc caccaggata
    39121 tctaatcatg ggaatgttca attcttttac caagtgaatg acgtcttggc gaaatccact
    39181 tttattagct gatggatgat ctggctgata aatcccttca tatactgctc gtcccatatg
    39241 ttcaataaat gaactgtaca accgcggatc aactttgcct acacgcttga cactactaat
    39301 caacattgtg gcttccataa ctgacctcct ttaatttata catgaataat acacctcgtt
    39361 gaaagcgttg tcaataatat aaatgttaaa cttacttatt ttttatcgat tttaattaac
    39421 caaaatatta ataatgatta aaacaaataa tttaatataa cttttattaa caaatatgtt
    39481 aatatataag aaaaaccttt taagaggtga tatatatgca actacaaata aacgaagaat
    39541 ccttaccggt atatgaagca ttagctagta aaacaaggat caagatcatc cagctacttt
    39601 ccaaaaaaaa gatgaatgtc aaagatttgg caaaagaact tggagtgagc agtgctatca
    39661 cgacgatgca cgtgaaaaag ctagaggaag caaacatcat caaaacagaa aaagtaggac
    39721 aacaaaaaat ttcgagcctt cgtgtcgata aaatcgatat tagttttcca gaaaaaatat
    39781 tcaatgcttt tgatacgaaa gaaacatcga tacctattgg tcactataca aattatgcaa
    39841 ttgaaccaac atgcggttta gctacgatcc atgattttat cgggaaagtg gatgaaccac
    39901 gctattttat ggatcctcga aggatggatg cccgcattct ctggtttacc agcggatttg
    39961 tcgagtatca ggcacctaat tttttaaata cagaggatac attggagatg ttggaagttt
    40021 ctgtggaaat ttcttctgaa tttccttttt caaatgacaa ttggccttca gatatcactt
    40081 tcagcctaaa tggtgtggag ctaggaacat ggacgagccc tggagatttt gctgatatcc
    40141 gcggtaaata tacgcctgat tggtatccag ataatcttaa tcaatacggc ttgctgaaga
    40201 cgatccgaat taccaaacac ttgacgaata tgaatggaga acccttgtca aacatcacga
    40261 tcaacgacat ccctaaagag caagacactt ggcatttgcg aatcgaagtg aaagacgatg
    40321 ccaaacatgt ggggggctgt actttatttg gtaaaggatt cggtaattac gatcaggata
    40381 tcaaattaaa agtttactac agctgatatt tttcttttct attttataaa acaaaaacag
    40441 ccaatcccta tgaagggacg gctgtttttg ctttattgtt atttccagaa atcatccaaa
    40501 acggaaattg gcaaatgtcg tttatgttgt gtcttgttcc accatgcttc gattttttct
    40561 tgtgcttgag cgggaacttc ttttccttct agataatcat caatttcatt atatgtgaca
    40621 cctagtgcga cttcatctgc aatcattggt ttcccatctt caagatctgc agtcggaatt
    40681 tttgtgtata atttttccgg tgcattcaat gcttgaagca attgtttccc ttgtcgtttg
    40741 tccaaacgga ataatggcaa aatgtcggct ccaccatcac caaatttcgt gaagaatcca
    40801 gtaatgtttt ctgcagcatg atctgtccct aatacggctc ctgctctttc tccagctacg
    40861 gcatattgcg taatcatacg ttgacgtgct ttgatatttc ctttgttaaa atcgctgatt
    40921 cccacacctg cttcgcttaa tacttgtact tgtgcatcaa cagctggttt gatatttact
    40981 cgcagactaa catcgggctg gataaagtca agagcagctt ttgcatcttc ttcatctgct
    41041 tgttcaccat aaggaagacg aacagcgata aattgatagt tttcatcttt tgtttcttca
    41101 cgcatttctt ccatcgttag ttgcgccaaa cggcctgcta aagtagaatc ttggccgccg
    41161 ctgataccta gtacaaaagt tttaagaaac ggatattttt tcaaatatgc tttcataaag
    41221 tcaatacttt tacgtatttc ttcttttgga tcaattgttg gtttgacacc taattcattg
    41281 ataatttctg tctgcaggct catttattct cctcctatgc ttctgtatga agttctgcta
    41341 cgcttttttt cattcgttcc atgatagcca ttttatggtt ccagcattca gtagacagat
    41401 cgactgggta tttttgcgga ttcaagtctc gtttatactc ttcccatagt gaatctagat
    41461 tttcttttgt atattctttg atttcgttta gtgcaggtaa ttcgtaaact ctttttcctt
    41521 cgataaagat atcttgtaaa actgggcgag ctgtaaagtc tcgaacagtt ttattgataa
    41581 acgtatgcac tggatggaac atgaagatag cttcttcttc tcgagggtct tcatcccata
    41641 atgtcacgta gtctccttca gatttcccat caaaatttcg agtgatgcgc cacacttgtt
    41701 ttttccctgg agtagttact ttttctgcat tgcttgataa cttgatcgta tccatcattt
    41761 ttccttcatc atcttcgatc gatactagct tgaatactgc accaagggct ggctgatcat
    41821 aggctgtgat caattttgtc ccaacacccc atacatcaat tttagctttt tgcatcttta
    41881 aattcaaaat cgttgcttca tccaagtcat tcgaagcata gatttttgct tccgtaaatc
    41941 cggctgcatc taattgttcc cgaacacgct tagatatata ggccatatct ccactgtcga
    42001 tgcgtacgcc ttggaagttg attttgtcac ctaattcttt cgccacgcga atcgcacttg
    42061 gcacacctga tttcaacgta tcataagtgt cgactaagaa cacgcagtct ttatgggttt
    42121 tagcgtaggc cataaatgct tcataatcat ttccataaga ttggactaac gaatgagcat
    42181 gggtcccgct agctggtatc ccaaatatct tcccagcacg tacattgctt gttgcatctg
    42241 ctccgccgat atatgccgct ctagttcccc agactgctgc atccagttct tgtgcccgtc
    42301 tagttccaaa ttccaatagt ggatcttctc cgatgacaga tttgatacga gctgcttttg
    42361 tcgcgatcaa cgtttggaaa ttgaccatat tcaacagtgc tgtttctact aattggcagt
    42421 gcgctaaagg cccttccact tggatcaatg gttcatttgc aaaaactagt tctccttcac
    42481 gagcagaacg aactgttgct ttaaattcaa aattttgtaa atactccaaa aaaccttcag
    42541 gatagacttc taatccacgc aagtaagcaa tatcggaatc attgaacgtc agattctcta
    42601 aataattgac taaccgttcc aatcctgcaa aaaccgcgta tccgtggtta aaaggcattt
    42661 ctcgaaagta acattcaaaa acagcatgta aatcagctcg acctaattcc cagtatgtct
    42721 gcatcatatt gatttgatat agatccgtat gcagcgtcaa actgtcgtct gggtaagtat
    42781 tattcatctt gtattactcc tcttggcaag ttttcatttc ctacccatta taacaaaaaa
    42841 aattaatagg aaaagaaatc ttccattttt tctgaaaaag cacatgtttt acggatattt
    42901 cttgctaaaa atgaactcca tctgctaaaa ctatcctttc ttattggtat ttaacctaaa
    42961 cgagaaagaa tacttatttt attcaatgta tccgagacac gaagaaatta gcgttttcta
    43021 tttttcttac ttgataaaat aagtagattt tcctactaca atcgtagccg ttaagatacc
    43081 gatgattgaa caaatataac caaatgtctc tacaacataa tcaatggcaa aagtttttga
    43141 agcaataata cgtctttcct tttttgcttc ttatccccaa agaaacggat ataacttatt
    43201 atgtattgaa tttttttgga aaggcgaatt atgacaagat ataccttgcg ttgaaaaaac
    43261 tcgcggaaaa aatatcacac gtttttaaaa agatacaaag taagaaagca gctgtcaata
    43321 gaatggaaac aaaaatggcg agacatcaat cagtctcgcc ttgtttttta ttgtatacat
    43381 tagatatgga gcaggttacc ataaagatga ttattagtcg ttattaaata gaatccttcg
    43441 tatttttttg ttctatcact acaatatctt tatttggacg tttgaattgt attccggcac
    43501 gttctagctc ctgtttcact cctacatgga ctttgacatc ttctcgtgtc atttttaaaa
    43561 cgtgatgaat cgcatagtcg atttcttgcc ttgtgatttt tttcttcgta ttgatcatca
    43621 cgacttgctt tttatcatat gtgtctacca atagcagttg tgttcgatct aacgaaaata
    43681 atttgtaata attaatagcc gtattcccaa aaaagaattc cactacttta ggataaaatg
    43741 tcttaatatt taaattacta ttcacaggtg tttcgattag cttcatctca ttcctctttt
    43801 ctatatagtc gatttagatc caatcttaag ttatgttacc atctttccta gcattaagga
    43861 atcggttaca attgaacctt taaagacaaa aggatttacc ttttatcact taatagtata
    43921 acataatttc atgagaaaaa aatacaattt tttcttgacc atctcgttac gtaaaggatt
    43981 atgcttacat ttgtcaggag gaaaaataat ggagtacaca ataaagaaaa tgtcagagat
    44041 atcaggagtc agtccgagaa ccttgcgctt ttatgatgag attggactgc taaaaccagc
    44101 taggatcaat tcttcaggtt atcggattta tggcaaaaaa gaagtcgatc gtctgcaaca
    44161 catcttattt tatcgaacaa tggcattcaa gttggatgat atccaagagg tcttagacaa
    44221 tccatctttt gaccaccaga aagctttaat caagcatcag caaatgttgc tggaaaaacg
    44281 ggcacaaatc gataccctct tgacaactgt ccaacagaca cttgatatgt acgaaggagg
    44341 aagaaaaatg agtgacaaag aaaaatttga agggtttaaa caacaaaagt tgaaagaaaa
    44401 tgaagaatcc tatggacaag agattcgaca aaaatacgga aaaaaaaagg ttacagcatc
    44461 caatgaaaaa tggatgaata tggataaaga aacgtatgaa gaaatgcaaa ctgttgaaaa
    44521 gcagatgtta tttgatctca ctctttactt aaaagaacca gaagatgaaa gcttagcaga
    44581 ccgtatcttc caagctcata agaagtggtt atcttacagc tggcctgaat atcagccaaa
    44641 agcgcataaa ggactaggaa tgatgtacga agccgatgaa cggttcactg catattatga
    44701 cgagcgaagt ggtcatggtg cagcaaaagc tttaaatgaa attatccaaa aaaaggcgta
    44761 atcttttaca tgatccaaca aaaaagtaca cgtaaaatga cctaataaca aaacagcaga
    44821 cacccaagtt agatttttat gctaactttt ggtgtctgct ccattctttc acaagttttt
    44881 tcatcctact ttaaatatat tcatcgttga ataaacgtat aaggtatttt cactttatcc
    44941 tttttttctg taagaaacaa ttgaaatgcc atttctccgc aagcttccag atggtggtcg
    45001 atggttggga aaccaagaag ctcgctgatc agcaaattat cgcgtccaat caccactggt
    45061 gtcttatctt gcagatagct ttgcaagatg cctgctgcaa ccatatccga atttgtaaag
    45121 acaccatcaa ctccaagctc tttaaaaaag aagcctgctt gttttccttt ctctgcatct
    45181 atacaatccc aaaaaacaag tgattcatca aaagatggaa aaagttgatg acatagctgc
    45241 aaagtgattt ttgagttata acttagcctg ccacttcgcc ccaacgtgat gcccaattgc
    45301 tttatacctt gctgcttcaa ataagtaaga ccttctgtaa ttgattgttc tcgatcaata
    45361 aagacacagc ttgcctgttt tcccttgatt tcttcacaaa aaactagagg tccatatttt
    45421 tgatatgcca ataaatcatc tagtgtattg gcacgagacg taatgatcaa tccatcgaaa
    45481 gctttagctg caaattcttc aagatacctt cgctcttttt ctttctgata gcccgtcggt
    45541 aaaagtgtca ctttgtaatc ataatcaaac gctgcttcaa tgatcccact gatcaactga
    45601 tcaaaatacg gatgattggt ataaggcaga atcactccaa tgcttttcgt ttttccataa
    45661 cttaactgtc tggcatttgc attagggata taatcgactt ctttcatgac cgcaagtatt
    45721 tctttccttt tttcctcatc tacatatgga tgtccattga ttacacgtga aatagtagaa
    45781 actgaataac ctgtgagctt tgcgatatct cgaatgtttg ccactgctgc ttcgccacct
    45841 atacaaaaag attttcttgt aaatagtcta ccacagctgg accgcattca aaaaactgat
    45901 acatttttcc ttcatgcgaa aaactgatcc gctgcccgcc ccaatatata tcttttgtca
    45961 aattttcgat ttcattcatt ggtatttctt ttactatatt cctattcaac ccttgaaaac
    46021 tgtacaacgt taatacggat cccttgaact cgacaaacgc ataaaaataa ttgttcgttg
    46081 tgccatctgt tatctctaca taagtattca ttgcaagccc tccttttgat actttgagta
    46141 tagtgtatga aacgcgtctc atagcaagga gtttgttttg ccaaagaaaa gttttattcc
    46201 tctttcatta ttaccaaagt ctcttatttt aaattgaata ca
//
LOCUS       DANKHL010000003        35991 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000003 DANKHL010000000
VERSION     DANKHL010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.003"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            190..870
                     /locus_tag="PW101_000107"
     CDS             190..870
                     /locus_tag="PW101_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2512541.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            960..1994
                     /locus_tag="PW101_000108"
     CDS             960..1994
                     /locus_tag="PW101_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL2512542.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            2008..2343
                     /locus_tag="PW101_000109"
     CDS             2008..2343
                     /locus_tag="PW101_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512543.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2327..4807
                     /locus_tag="PW101_000110"
     CDS             2327..4807
                     /locus_tag="PW101_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512544.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            4863..6422
                     /locus_tag="PW101_000111"
     CDS             4863..6422
                     /locus_tag="PW101_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2512545.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6419..7783
                     /locus_tag="PW101_000112"
     CDS             6419..7783
                     /locus_tag="PW101_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2512546.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7954..9024
                     /locus_tag="PW101_000113"
     CDS             7954..9024
                     /locus_tag="PW101_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2512547.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9039..9707
                     /locus_tag="PW101_000114"
     CDS             9039..9707
                     /locus_tag="PW101_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2512548.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9727..10305
                     /locus_tag="PW101_000115"
     CDS             9727..10305
                     /locus_tag="PW101_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2512549.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10706..11617
                     /locus_tag="PW101_000116"
     CDS             10706..11617
                     /locus_tag="PW101_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2512550.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11705..12622)
                     /locus_tag="PW101_000117"
     CDS             complement(11705..12622)
                     /locus_tag="PW101_000117"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDL2512551.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            12824..13261
                     /gene="zapA"
                     /locus_tag="PW101_000118"
     CDS             12824..13261
                     /gene="zapA"
                     /locus_tag="PW101_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDL2512552.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13320..13868
                     /locus_tag="PW101_000119"
     CDS             13320..13868
                     /locus_tag="PW101_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDL2512553.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            13983..16343
                     /locus_tag="PW101_000120"
     CDS             13983..16343
                     /locus_tag="PW101_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDL2512554.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16430..16744
                     /gene="trxA"
                     /locus_tag="PW101_000121"
     CDS             16430..16744
                     /gene="trxA"
                     /locus_tag="PW101_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL2512555.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            16926..18704
                     /gene="uvrC"
                     /locus_tag="PW101_000122"
     CDS             16926..18704
                     /gene="uvrC"
                     /locus_tag="PW101_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDL2512556.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            18849..19748
                     /locus_tag="PW101_000123"
     CDS             18849..19748
                     /locus_tag="PW101_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2512557.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            19741..21003
                     /locus_tag="PW101_000124"
     CDS             19741..21003
                     /locus_tag="PW101_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2512558.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21164..23062
                     /locus_tag="PW101_000125"
     CDS             21164..23062
                     /locus_tag="PW101_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HDL2512559.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23392..24111)
                     /locus_tag="PW101_000126"
     CDS             complement(23392..24111)
                     /locus_tag="PW101_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDL2512560.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            24448..24780
                     /locus_tag="PW101_000127"
     CDS             24448..24780
                     /locus_tag="PW101_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512561.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            24767..26770
                     /locus_tag="PW101_000128"
     CDS             24767..26770
                     /locus_tag="PW101_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDL2512562.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            26789..27259
                     /locus_tag="PW101_000129"
     CDS             26789..27259
                     /locus_tag="PW101_000129"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDL2512563.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27280..27864
                     /locus_tag="PW101_000130"
     CDS             27280..27864
                     /locus_tag="PW101_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HDL2512564.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            27879..28865
                     /locus_tag="PW101_000131"
     CDS             27879..28865
                     /locus_tag="PW101_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HDL2512565.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            28855..29166
                     /locus_tag="PW101_000132"
     CDS             28855..29166
                     /locus_tag="PW101_000132"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDL2512566.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29188..30969
                     /locus_tag="PW101_000133"
     CDS             29188..30969
                     /locus_tag="PW101_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDL2512567.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            30962..32338
                     /locus_tag="PW101_000134"
     CDS             30962..32338
                     /locus_tag="PW101_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDL2512568.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32345..32980
                     /locus_tag="PW101_000135"
     CDS             32345..32980
                     /locus_tag="PW101_000135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDL2512569.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33010..34365
                     /locus_tag="PW101_000136"
     CDS             33010..34365
                     /locus_tag="PW101_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDL2512570.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34557..35951)
                     /locus_tag="PW101_000137"
     CDS             complement(34557..35951)
                     /locus_tag="PW101_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL2512571.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
BASE COUNT        11892 a         6171 c         7660 g        10268 t
ORIGIN      
        1 aaaaaataaa attgaaatat tttttcatac ttaataaaaa aggaagaata ttcatgtaaa
       61 cgattttatt gtttttttct cttttataca aaaagggtca taatttcaag taagcggatg
      121 aattcatgag gattttttgc atactgtctc tactatactt aggtcactaa gaaaaaggag
      181 tgatttatta tgaaaaagaa catgattggg acattattac tttcaacact tcttctaggc
      241 ggcttggcaa caccagcttt tgctgagggt caagcgactt caaaaggaga tattaccttt
      301 actgaaccaa caaataccgt ggagcctctt aaccctactg atccttcaaa accagtagag
      361 ccagcagatc cggaaaatcc agcaactggc cagacaggtc ctttaacact ggatgtagtt
      421 cccgaattgc catttggtac acacgagatt gaaagcggaa caaaaacgta tcaagtagac
      481 gcttcaaaaa acgatacacc ttatctccaa gtatcagatc gtcgaggtgt aggtgcagat
      541 ggccaagcac aagggtggaa cgtgacagtt tcggtttcag attttgtaaa cggttcacaa
      601 gttttgcaag gcgctgaatt agattttgga acatccactg ttaaaagtac gtcagataac
      661 gaatccactg gaccaactag tcaaactgta actgggttga gtaaagcaag tgcagcgaca
      721 ccaatattta cagcagcaaa agatcaaggt ctgggaacat ggctttctgt ttatgatcca
      781 gcaaatatca cactgaaagt gccgaaagca gcagctggaa cattcacggc tgatttgact
      841 tggaacctcg tggctggccc agtagcatag agtatgaaat aaattcaaca aactgcggtg
      901 aaaatactat aggtgaagga ttttttaccg cagtttttgt tgaaaggatg tggtggacaa
      961 tgaatcaaag taaacgactg tttgtttcgt ttttttctat tttaagtcta tttttcattt
     1021 ttccgtcaat cagccaagca aaggattcag caggagactt tggaatcaaa ccggtatttc
     1081 cagagaacca aatagataaa gcaatcggtt attttgattt attagtggca ccggaacaaa
     1141 atcagacact agaagtaatt atcagcaatt cttcagatga agaacgaaca tttgaggtct
     1201 ctgtcaatcc ggcggtaacg agtgatggtg gaacgatcga ttatagtcaa aaaaacccaa
     1261 cgttagatga gacattgccg tttgatgtcc gtgacgtttt gctcattgcg aaaaaggaaa
     1321 tcaatgtatc agcacatgca gaaacaactg tacctataga agtgaaaatc ccagcaaaaa
     1381 gctttaaggg acgtgtatta gcgggtattc atgtttcacc taaagaagaa gctgaaacag
     1441 aaaatgctac agaaggagca caaatcaaaa atcgtattgc ttataatctc gcagtcgttt
     1501 tacaagaaag tcaagagacg atagaaccgg atctgaaact tttatcagga gatttggacg
     1561 aggtcaacgc aaagccaact gttcagcttc gtttccaaaa tcctcagcct aggattatca
     1621 gcaatttgat atttacgagc aagatctttt atgaaaatca gctttatatt gaaaatacgt
     1681 ctaatgcatt tttagttgca ccaaattcga attttcactt gaatcttgat ttagctggtg
     1741 ataaagcaaa agccggcgat taccgcgcag aaatcatagc aaaatcaggc gacagtaatg
     1801 aatggcgatt tacacaaaac tttacgatca aaaaagaaaa agcacaaaaa gtaaatgaaa
     1861 attccgtttt tgcagtacaa gaacaatctt tcccatggct ttatgcagga ttggttgctg
     1921 cagttctagt gttatggttg attttgctgt ttttgtgggt ccacaagaaa aagaaaaaag
     1981 gagaaacaac atgatgaggt gatttcgttg aaaacatcga tcttattggg agggattatt
     2041 gcaagtttgc tgttcttcag ctggcaagag ccagtccaag cagataccaa aagttcaggt
     2101 acgattacat tttatgatct aagtccaatt ccgcatccaa gtggttcagt caatggcgca
     2161 tcaaaacagc agacacagct accgatttat aaagaatcta atcaacatgg cgtattcccg
     2221 gagacgaatg aacaaaagat ccgttttctt tatttgatca ttggtataga attatggctt
     2281 cttgcgtggt tgttgttatg gagaagaaag gaaaaggagg atgaacatgt tgctacaaaa
     2341 taaattttca ttttttcgct ccttcttttt tacttcgctt tttgttttaa gtatatttat
     2401 cttttcacct tcggcagaag ctgttggagc aacgcctcct gccatttctt atgatccata
     2461 cacgtatgaa aatatcgtac ctccaaatgg tggacgtagt gcagtcaatg gcgggaagct
     2521 tgtccaacta actggggcta accaattgcc taaattgact gctacttatt tgaacgatgg
     2581 ttcaaatgat ccttggaaag gtggcacggt ccgttttcat tgggaaagcc atgtgaaact
     2641 aacaaatgta cttgatttgg tgaatatcac tgcacaagga tttgcatttg tggcgatcat
     2701 caatttgccg gaacatgtat caagcagtga tgtattaaaa gcaattgact ggaacaacgc
     2761 ctacttgtat attgccggtg cccccatcaa ggttactagc ggaagtttat tatcagcagg
     2821 aagccatacc ttgcgttttg ggatggggtc gtgggataca aatactctat tgacagatat
     2881 tattgctatt attacgcgag gcgaattcaa cgtggacgat atcccgcttc aatttgatgt
     2941 tacagctaat gtcgcgaaga tgacagaaaa cggagataaa tatgatacga gtccaaacaa
     3001 aattttgacc aagggtaaat tacaaccttc tatgtctaaa aaggccgttt tttctgttga
     3061 tttttatgat tcgaataata ttatccaaga tcgtatgttc aattcgattt tagctccagc
     3121 tgcaggtaga ttttatccta aattaactgg cggaaacttg aatccaggag catatgcatt
     3181 ttctgcaagt gcagagataa atacgtggaa tagttacatc tcaccaagtg attcaacagg
     3241 aaaatataat acatttgctg ataccacaga atacgtggat ggaaataacc agaaccttcc
     3301 aggctctacc aatagtcgga cattgacgat ggggctgact gaaggacgtt ttgatcagtt
     3361 gccatttagt cgctttgatc gcgtagtcaa ctattttaca ggaaaaaacg ttacacaagg
     3421 agcaatactg actcatactc cagcaagcac aagtggattg aatcagacag ccaatatcgt
     3481 gtacagtggg aaagatgcgc aaggcggatt gctttctcct gttgctttaa aggtattgga
     3541 gcagtacaag ctagacggaa cggttttacc aacaaactta acagccgata gtcagtgggg
     3601 aaagctcctt tcttctgcat cgtaccaagt cacggccaat tggtcagcaa ctgaactaaa
     3661 aaagggttcg atccattaca ggctttattc gaaaggaacc caaaaattag cgacaggatg
     3721 gacagatcaa cttttccaaa cgatccaatc aaataacggg ggtgtaaata cagctacttc
     3781 tacactacct gcattggctt cagggcaata ttattttgat tatcgcttgg tggacgatgt
     3841 acttactaac atgtatccgt cttttgcgta caaatggcag tctgaacaag ctggaatcac
     3901 cactcttcct caaataacag tggctaattt tccatctgtg agtgcagata gtcagctaaa
     3961 aaaccttagc agacaacctg agacaggaga tcccctgtct gctctatcag gagatacgat
     4021 tcaagaaaat gtgacattca cgttaacaaa atctggggat gcgataaccg acaaaaaaat
     4081 cacgatctca cttcctgaaa atactactta tatcaatggt tccttaaaat taaatggtac
     4141 aactatcgca gataccggca ttcaacaagg agtttctgtc ccagcagatc tattgagcaa
     4201 aataggagat accattcatc ttacttacaa ctatcaatta aatactgtag atacttcttt
     4261 acaatccgtc agcatactga caaaggcagc tgtactttcc agcaatatta cattagcaga
     4321 tggtgctaag cttcctaacc cagttgtcca aacatctgct aaaacaattc ttgtccccaa
     4381 acaagaatta acattagtta atgtaccaga tgattttaca tttggtaacg atctccccaa
     4441 accgttgaag acaagttatt atgaatcaaa aggcgatttt tcttttgatg tgcgggatac
     4501 tcggcttcca agcacgtctc cttggcaact gacaggtaca ttgacttctt tatttaagaa
     4561 tgatcaaggt caagaattaa gcggaacaaa attatacttc aatcattctg gatcaaagca
     4621 attgattcag caaggtcaaa acacgttgat ttatgagagt gatggaacag ctaaaggaga
     4681 ggttctcgtt gactttcccg ataccgatgg tttactctta gaggtaaata gcagtacgaa
     4741 tgctcaacct ggcgcaacgt atcaaggcat ggtcacatgg aaactgacag ctgggccaac
     4801 cagctaagtg attagaattt gtatggatct gtgataactg tcacagatcc tctttttcaa
     4861 ggatgagaag gaaacttctg ctgttaaaat cagaaatgct tgaaggagtg gacttggaac
     4921 ttttcagttt gcttgaaaaa aatgaacaac ggcaaatcaa acttttccaa cttttattag
     4981 aggaaaaagg agaggtttcc gttaataacg tacttacccg cttatcagtt gatcgggcta
     5041 ccttgaaaga agatctgttt tatttacaag ctaacttgga aaatatcaaa caattggaaa
     5101 ttgttatcca agaagggaat attttattaa tcagacatgg aaatatagca gcagtcgatg
     5161 tttattatca ttgttacttg cgctattcta caaaatatca gatacttctt tatctgttgg
     5221 aaaatggctc atttgatcgg cagaaacttg tcatggaact taatatcagt tcggctactt
     5281 tgtctcgcag agtgaaggaa ttaaatgatt ctttaaatga gtttcatctg aaaataaaaa
     5341 gcgggcggct gaccggtcca gaaagtcaaa ttcgttattt ttatttccaa ttattttggt
     5401 ttggacgtcc ctatcttgaa aacaaaaaag aatacagtga tttatcagga aacacttttt
     5461 tagatctgct gcaaaaagag tttagttttg ctattacaga agaaggacga ttgaaatttg
     5521 aaatatggct ttccattatg aagaagcgaa tgcgctgtac gaaaaaatcc aaaccagatt
     5581 atagcaatct tcctgttgga ttgtttcaaa cgacacaatt tttattggac ctccatacat
     5641 ttttgacccg ttacttcttt caatatgcct ttccttggaa tcaaaatgaa acgttggttt
     5701 tttatctttt catgatcagt aactttacat tagatacaca taactcgcat gtccaggagt
     5761 ttttgataag cgaaaaatac aaagacgacg tagtagctca attgaatcga acgtttaaac
     5821 aatcgataga agaatatttc tctttttatg tttttccaga agagatttcg caaaagattg
     5881 cgttgacagt gatgcaaaat cacgctcatt ttgcctattt tagaggatgg atgaatattt
     5941 ttggccaaga aggattaaaa cagcggttaa ctgaagtaga agataaacaa ttcctggttt
     6001 tatgtgaaag attggtcaga caaacatttg ctattgttga ttttactgaa gtagacgagg
     6061 aaaatgcccg ctttgaatta tttgctcgct atgcatctat tttacggacg ctgatcaagc
     6121 agttagaatt acgtctgcaa attgcttatg atcttcctta tgaaaggcta atggcggata
     6181 tcgtgaaaga aatcatccag gaacaattgc tgaatacgat caaatatgat ttagtcgaat
     6241 acgagaaaga aaagcagtat gatatcattt tgactagcca attgaaagag tacccgtcac
     6301 agaagtccgc taaagttttt gtattcacta gcaacgaata taattatgat ttcccctatt
     6361 tgaaccaatt tttaaaagaa tgttatttag aaaaattgaa tttaaggatg aataaaacat
     6421 gaaaatcgtg atcattggtg cttcccatgc aggcatcaca gctgccctaa atctaagaaa
     6481 acttcaaccg aaagcagaag ttttactgat cgatgaagat cataaagatg gactaggcta
     6541 tgtttcaaat ggaatcaacc tttatttaaa aggaaagata cgtagtttag ctgaagttgc
     6601 ccataacatg cggactcttc aagaaagtgg ggcaaaactg atcaccgaat ggagagtaac
     6661 tgaacttgat ccagacaaac atcagctcat cctcgcatcc aaagaaggaa aaaacgagac
     6721 cataacctat gataaattaa tcgtagcgac aggttcctct cctgtcacct tgtataaaca
     6781 aattgaagct gaaaatgtct acacctataa aaatctagtg caatctaagc aagtacttgc
     6841 cgctttaaaa gaagcaaaag aagtagtgat cttcggtgct ggttacattg gattggaatt
     6901 agcagatgct ttgagaaata aaggatacat tattcatcta gttgattata tgccgaatgt
     6961 tttatcaaga tatttcgata aggatatgat caacagtttt caaaatcagc tgcaaactaa
     7021 gcaaataaat ttttatccaa atgaattctt gatcgattgg aaaaaatcag aagagaaagt
     7081 agtatccgta caacttctgt ctcaaacaat aaaggcagat atggtcattt tttctgccca
     7141 aactcgtcct aatacgacat tgttaaaaga aaaagtggct ttgtatgagg atgagactgt
     7201 gatagtcaat gaatatctgc agacaagtga tccggacatc tatgcgattg gagatatcgt
     7261 cccagtcagt tttgacaaaa ataaacggca tctcttttta cctttagtta ctcgagctgt
     7321 acatatggca agagcagtag cgctaacttt gagtggtcag ccaaccgctt acgacctcag
     7381 acaaaagata acagcagcag tcattataga ctacttttta ggaactgtag ggctgacaga
     7441 agatgaagcg ccattcttgg aacaaaatac cggttcgtgt tcaggagaat ttgatctatt
     7501 tccacaatac gacgaagaaa acaaaacagt aaatgcaaag ctgatctacc atccggatac
     7561 attagaaata attggcggac agctgataag ccaagaattc ttattatcag atttgaacct
     7621 gcttgcggat atcgtgaaac acaaaactac gattccacag ctagcagttg aggattttgg
     7681 atttctgtct gaatacacct ctcgttttca ctacctcaat gagttagctt ttaaagtact
     7741 agctaagaga gcaaatagca atagagtaga taaaagactg tgagaaatat cacggtcttt
     7801 tattgtgtgg ggatataata aatattaatc aatcgagaat aaaaaattca tataatgaaa
     7861 tagcggaaga attttttgag cataatattc gccaaaaatt tatcaagtga taaaattgtt
     7921 gtataaaatg attatttcag gaggaggagt taaatgtttt tagcattaaa tgaaattatg
     7981 cattcaaagt tacgttatgc gttagttgca ggggtgatgt ttttgatcgc ctacctggta
     8041 ttttttttga ctgggttagc ttatggctta gctcaagata atcgaacagc agtcgacaag
     8101 tgggaggcag attcgattgt gctttcgaaa gatgcaaatt caaatctagg gatgtccatg
     8161 attactaaga agatagcaga agaagtcgaa ggtgggaaag tggcgtatct tgcccaaaca
     8221 cctggagtcg tgactagtaa agacagtaca gaagaaggaa aaatcaatgt tagcttcttc
     8281 ggtatcgata aaaatcaatt catcatgccc aatcttgttg aaggaaaagc ttttgataac
     8341 gatgatgagg cagtaggcga tattagtttg aaagaagaat atggtctagc cataggagat
     8401 acagttaagt tatctggtag tgacaagacg tttaaactaa ctggctttac ggatcatgcc
     8461 aaattcaatg tctcaccagt tctctataca accatcaatg cttaccaaga aatccgcttc
     8521 gaaaaagaag atacaagtga aaatgcacgg attaacgcaa tagttgtcag aggtaaaatc
     8581 agtaatttac ctgaagattt agaacagatc aaaatttcta aattcatcaa tgaattacct
     8641 ggatataatg cgcaagtatt gacttttgga tttatgatcg gatttttgat cgttattgcg
     8701 gctattgtga tcgggatttt catttatgta ctgacgatgc aaaagattaa tatctttggt
     8761 gtaatgaaag cacaagggat cacaggagga tttattgcgc gttctgtagt ggcacaaaca
     8821 ttcattttat catttgttgg tattcttctt ggtctattag gaacagtggg aacatccctt
     8881 gttctacctg atgcggttcc attccaaagc aattggctgt tttttggcgt cattagcttg
     8941 ttgatgttag tagtagctgt gttaggcgca ctattttctg ttcggacaat cgtaaaaatc
     9001 gatccgttga aggcaattgg ataagaagga ggaagaacat gaaagcaata gaatttaaag
     9061 aagtagaaaa aagtttccaa gatggcgacc aagtagttaa agcattgaaa gaaacaacca
     9121 tgagtatcga taaaggggaa tttgttgcaa tcatcggtcc ttctggttca ggtaaaagta
     9181 cttttttgac tttggcaggc ggattgcaga cgcctagcag tggctctgta tttatcaatg
     9241 atcaagaatt cagtaatcaa aaagaaagcg aacgcgttaa actacgtttt aaagaaattg
     9301 gctttatttt gcaagcttcc aatctggttc cttttctgac agtaaaaaag cagttattat
     9361 tagtagataa gattaaacat gaacatcgtc aaaaagaagc aaacgaatta tttgagcagc
     9421 ttggcgtgga taagttgatc aacaagtatc cagaagaact atctggtgga gaacggcaaa
     9481 gggtcgcaat cgctcgtgca ttgtacaatg atccgtccat tattctagca gatgaaccaa
     9541 ctgctagttt ggattcagaa aaggcacgag aagtagtcga tatcttggca aaagaatcaa
     9601 aagacaaaaa taaagcaacg atcatggtta ctcacgatac acggctgatt gacaaatgcg
     9661 atagaatatt tatgatggaa gatggtgtat tgaaagaaaa agaataacca aaaagcaggt
     9721 ggaaaaatgg tccatcgata caacaaagat acacagcaaa aaattttgaa agaagcgact
     9781 gacttgttta tgtccaaggg atatctcggc acttcaacaa gagaaatcgc acaaaaagtt
     9841 gggatcaccc agcccaatct ctatcattat ttcggtgata aagaaaaact atatacggca
     9901 gttttagaaa accacttaaa agatgttgga aaagctttga gagaaatcgt tcaaacaagc
     9961 gaaacgggat ttcaagtaac cttgacaaaa atggcgcagt ttttgattga gacacatctg
    10021 gtcgatttgt ttctgatgct tcacgatctt gaaagcaatc tttcaaagga aacaagagat
    10081 catcttttct ttctatggaa gaaaaattat cgtgaacctt ttgaagaaat cttttcagca
    10141 aaccaatctg tgctaagaaa tgggatttca aaagaaatcg cagcaaggca ttttttctta
    10201 gtgttagcac cttacatcac tcggtcgaaa agacctgtgg aagaaccgct aaatgtggcg
    10261 caattgatcg atttatattt gaatggtgta ttaggagaag cgtaaagctg ttatataggg
    10321 agaaaatagt taggtgaaga agcaagatat gacgaagatc gttatatctt gcttctttat
    10381 ataggtagtt tttcataaag acaaatttgt tattctctca tgattatcag ggtatatttt
    10441 tatttattat gtcggtgttc caggaagtgt tttaatttaa taacgaagaa attaattgtt
    10501 tgtagaatga aggaaactat ttattgggta gatgaatcac cacgaatagt caaaaggaga
    10561 tttcaggata tgccaaaaat gaaaaaaatc attttagcag taatgttatt ttctacggtt
    10621 atatctttta cagccccttt tttgcttact taagctgatt ttaagttttg gcccttagtc
    10681 tggatctacg aggtgagagt caagaatgaa aaaaataatt ggagcttttg tatttgcaat
    10741 cattatcatt attatctgtt ttcaaataga tgtttttggt caaaaaaaga tagttggaca
    10801 aacggtcagc agtacgaaca gtacggttac aaagacaaag gaatcatttg atgatacaga
    10861 acaagagaca ttaatcaaaa tcggaaaaaa tgaccgtaca ctatatacag atgactcgct
    10921 aactacccca cttgcaacta taaacggcgg agagctggcg gagtttcttt ctgaaacaaa
    10981 aaatgcttat cagatcaaga caaatgatgg ctatactgga tatttagccc tcgaagatgg
    11041 aaccaaagtc acaaagaata tacaaacaaa accggaagag ctatctgatg cagtgattgt
    11101 attagatcct gggcatggcg gaaatgatac tggggcatta agcaatgatg aacaaacaga
    11161 agaaaaagat ataacattaa gtacagccat aaaagtaaaa aaggcgctag aggatgctgg
    11221 agcaacggtt tatttgaccc atactacaga tgaattagta cagcttggag atatatgcga
    11281 ctacagtgaa gaaaaaaagg cagatgtctt tatcagttta cacgcagact ctacggaata
    11341 tgcgaatgaa gcaactggaa tcacgacata ttattactat ggacaagagg aaacattagc
    11401 acagacaatt gctgactcct tcactgatct gcctctggat tcaagaggaa tctccactgg
    11461 gaactatcaa gttcttcgag aaaatcttca accttctatt ttaatcgaga tgggttatat
    11521 gaataatgat agtgatttaa aggaattagt aacagacagt tatcagcagc agattgctgc
    11581 aagtttgaca caagcattga ccacttattt tcagtaataa ataagcccag ccaaacaaaa
    11641 atagctcctc atatttttcg tttggccggg cttattatcg cagatgcaag cttttgaaca
    11701 ccgtttattt ttttagtagc ttcaaagctt tttctgtatt tgcaaagtga agtttggcat
    11761 atttttcaag cataggcatg ccgcctttct ccaatagttt tgccgctact tggtcagatt
    11821 tcgttccagc tcctgatgga agggtgattc cagcttcagc tgatgctttg tgtaactctt
    11881 ccaaaaagct ggcacgactg atgatagaag cagcagcaac agctaaatga tattgttctc
    11941 ctttagtaac gaagtacagt ttttcttgta attgattttt ttctaaacgc gcatattttt
    12001 taaaatttgc ttccggcgta aactgatcga tcaaaatcgc ttctggtttt tctggctcga
    12061 ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc gactttcatc cgaacggcat
    12121 tgtattttgg ttggatctca ttgtattttt ctggagataa gacaagcagt ttatagggga
    12181 tggttttttt taagatgtca gcaagctgga tgatttggct atcttttaac tcttttgaat
    12241 ctctaacacc aagtttacgt aattctgcaa gctgatcacg tttcacataa gcagcacata
    12301 ctgttagcgg accgaaatag ctgccatttc cgacctcatc actacctaaa acggataatt
    12361 gactgatatt ttttggcaaa gaagaggctg tttttgctgg cttggcttgt ttatcctgtg
    12421 cctcccccca tttatcagct tctattgctg cattcgtacc ttgaaacatc acttttccgg
    12481 atgtataagc ggtaatggtc gtcccgtctt tttttgcagc aaagctagta taaggaatct
    12541 gtttatttaa caaataagag cgatagtatg ctttcatttg actaagttgg tcgtttgttg
    12601 ctttgataat gacgttgctc ataaaattct cctcgttttt atacataaaa ctataccgca
    12661 actcacgact tttcgttttc taagagattg cgagacatag cattttttta aagtttatca
    12721 tataatagtt aagtatgtga aaaatatttg tggagaacgg tgaaagaacc aacttcatgg
    12781 tacactaaat aaaactttca tcaaatagga ggaaatgatt cgtatggcgc acgaaaaaac
    12841 acgctacaaa gcggtgattg ccaaccaaac atatactatt atcggacggg aaaccaagca
    12901 tcatatggat atcgtaacga agctgatcaa tgaacagtta gcagaattga aacaattatc
    12961 accacaaatg gataatgaac aagcagcaat cttgatggct gtcaatgcgc tttcggatca
    13021 attgaagaaa caagaaagaa tcttggagct ggaagaagaa acagcagaat taaagaaaaa
    13081 aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc attgaagcaa tcgagaacga
    13141 agctcgggaa gtattgaaag aaaatggtca agcagatcat accattaaaa atcatgtgga
    13201 agcacaacag attttaaacg agaaacgaaa aggacaaatt aaacaaaaat cttctaatta
    13261 agatcctttt atcaaaataa gaaaaattta aaaaaagtta gaagagaaag gaacgtagca
    13321 tgctgagttt gctgatttta tttttattgc taattgcgtt tttttctgga gcacgtcgcg
    13381 ggtttgcttt gcaaatcata ttcgcgattg gatatgtatt gtcatttatt gctgcccagc
    13441 atttttacaa accattagca agtcatttgg aattatacat tccataccca gctgtcacgc
    13501 caacatcaaa gttagcattt tttgatcagg tatttgcctt tcacctagat gaagcttttt
    13561 atgcaggaac tgcttttctg attattttgc taattggctg gctgcttacc cgatttgtgg
    13621 gggtttttgt ccatggattg acgtatgtgc cgatcttgag acaggtagac tggattgcag
    13681 gaggcatcct gtcactaatc atggcttacg ttactatatt tttgattttg cagttacttg
    13741 cctttgttcc attagatgtg gtccaaaatc aattcggcga gcatagtttc gctcgcttta
    13801 tcgttgagag aacgccattt cttaccaaca agatgtatga gctttgggta acgaatattt
    13861 tgaattaagg aaaatagtgc tggaaaatat ttcgtttttt gtacagagta gattatttaa
    13921 gaaggctgcg tagcgtatgt ccggcacagc tcttttttca tagtgaaaga taaggtgata
    13981 aaatgaataa ccgaatcctt gaaacattgg aatttgaaaa agtcaaacag atggtcagac
    14041 aatttgtagt gactgcacaa ggaaaagaag aactggcaga attggtacca gtgagcgagg
    14101 aacaaacgat cacaaactgg cttcaagaaa ctgaggatgg attgaaagtg caacgtctgc
    14161 gaggcgggat ccctatacct aaacttgaaa atatccgtcc ccatatgaaa cggattgaga
    14221 tcggagcaga cttgaatgga gtggagcttg cacaagtttc cagagtattg tctacgacaa
    14281 gcgaattgaa tcgtttcatt gatgatcttt atgacagtga aatcgaattt gcacgtttgt
    14341 atatgtgggc agatcagtta gtcacgatcc ctgttttgag ccgccgttta aaggaagcaa
    14401 tcgatgaaga cggacgcgtg actgatgacg cttctcctga gttaaagagc ataagacaga
    14461 acattcggcg aagtgaacaa gctgttcgtg aacagttaga tgggatcgtc cgtgggaaaa
    14521 atgcaaagta tttaagtgat gcgatcatca ccatgcgaaa tgaccgctac gttatcccag
    14581 tcaaacaaga ataccgcggt gtatttggcg gtgtagtcca cgatcaaagt gcttcaggac
    14641 agactttatt tattgaacca aaacaagtgg tggatttgaa taatcgtttg cgtcaatacc
    14701 aaatcgctga acgaaatgag attcaacgta ttttaagtga actttctgct gaacttgttc
    14761 cccatcgtca agaaatcatc cacaatgctt atgtgattgg taaaatggat ctgatgaatg
    14821 caaaagcacg atttggtaaa gaagtaaaag ctatcgtgcc gggaatcagt gaagacaatc
    14881 atgtcgtctt gaaacaagca agacatccac tgatcgatca ggaaaaagta gtttctaatg
    14941 acatcacaat cggaaaagac tatcaagcca tcgtcattac aggacctaac acaggtggga
    15001 aaacgatcac gttgaagact ttaggtctgt tacaactgat ggggcaagca ggattaccga
    15061 ttcctgcagg tgaagaaagt caaatcggta tttttgagga agtatttgcg gatataggag
    15121 acgaacagtc gattgagcaa agtctatcga ccttttcttc tcatatgacg aacactgtag
    15181 atattttatc aaaagtcaat gaaaaaagtc tggtattatt tgacgagctc ggtgcaggaa
    15241 cagatccgca agaaggtgct gctctagcaa tcgcaatctt agatgatctt ggtaaaaagt
    15301 ctgcttatgt gatggcaaca acgcattatc ctgaattgaa agtttatgga tataatcggg
    15361 caaatacgat taatgccagc atggagtttg atgtggatac attaagtcca acgtatcgtt
    15421 tgctgattgg cgttcctgga cgaagcaatg cgtttgaaat ctctagccga ttaggtcttg
    15481 atacagaagt tattgatgaa gcgaagcagt tgatgaatga tgagagccaa gatttaaacg
    15541 aaatgatcac agatttagag aatcgccgga aaatggcaga gaccgaatat ctggaaatgc
    15601 gtcattttgt ttctgaggca caagaacttc acgatgattt gaaagaagct tacagctatt
    15661 tctttgaaga acgtgaaaaa gaaatggaaa aagcaaagaa aaaagcaaat gaagtcgtgt
    15721 cagaggcgga agaaaaagca gaaaaaatca ttgctgatat tcgtaaaatg caacagcaga
    15781 ttgggcaagg aaatgtgaaa gagcatcagt tgatcgatgc aaagacccaa ttagcaaatc
    15841 tacatcaaga agaaactttg aagaaaaata aagtattgaa gaaggcgaaa gaacaaaaaa
    15901 cgttgaaacc gggagacgaa gtgttggtta cgacttacgg ccaaagaggg acgcttttac
    15961 gtaaaaacgg gaatcagtgg caagtagaaa tcggtatatt aaaaatgaat gtgtccgaag
    16021 acgagctgac gcctgttgct cctcaaaaag agccgacaca acgagttatc catgctgtac
    16081 gatctgaatc tagtagtcac gtgccgaatc aattggacct tcgaggaaaa cgatacgaag
    16141 aagcgctaag tgaggtagat caatatttgg attctgccat tctagctggt tatcctcaag
    16201 tgactattgt ccacggaaaa ggaacaggcg cacttcgcaa gggaattact gattatctta
    16261 aaaaccatcg gagcgtcaaa agctttgagt ttgcaccggc aaaccaaggc ggaaatggtg
    16321 cgacaattgt taaatttaag taaagcttac taaaggtaag cagatggttc gctatccaag
    16381 tatagaaatg ctatactagt catagatttt aaaaatggag gtctttgata tgtcacaagt
    16441 aattacagat aagacattta atgaagaaac agataaagga cttgtattga ttgatttttg
    16501 ggcaacttgg tgtggtccat gtcgtatgca agcaccgatt ttggatcagt tggaacaaga
    16561 atatgatgaa gaagaattca gaattgctaa aatggatgtg gatgaaaatc cagaaactcc
    16621 tcaacaattc ggtatcatga gcattccaac attaatgttg aaaaaagatg gtcaagtagt
    16681 agaaaaagct gttggtgttc attcaaaaga acaactacgt caaatgatcg atcaatattt
    16741 ataaaaactt acagcaaaaa taagctgagg gtgggactga atccagttcc gtcctcggtt
    16801 tttttgcggt tgagttaagg ttattcaaat taatattttt tgagtataat tacgttgtca
    16861 ggctggattg tttttttgac agacatgcta taataacttt aattgaatat gaaggggagt
    16921 accagatgaa cgaacgaatc aaaaacaaat tagctttact tcctgatcag cccggctgtt
    16981 acttgatgaa agataaaaat ggaacgatca tttatgtcgg caaagccaaa atattaaaaa
    17041 atcgtgtccg ctcatatttt actggtagcc ataatacgaa aacggaacgg cttgttagtg
    17101 aaatcgaaga ctttgaatac atcgttacag aatcgaatat cgaagcattg ctcttagaga
    17161 tcaatctgat aaagaaaaat gatcctaaat acaacattat gttaaaggat gataaaacct
    17221 atcctttcct taagataacg aatgaaaaat atccccgttt ggtgattact cgtaaggtac
    17281 taaaagacaa agcactgtat tttggaccat atcccgatgt cggtgcggcg aatgagacaa
    17341 agaagatcct cgatcgatta tttcctctga gaaaatgcaa acctagtcag acgaaggagc
    17401 cttgtcttta ctatcatttg ggacaatgtc tgtgtcctta ttatttcgac gtagatccga
    17461 aagtctatac gaaaatcgtt gatgaagtga aaaacttttt aaacggagga cacgaaaaaa
    17521 tcgagtcaga gatccatcag aaaatggaaa aagctgctga aaacatggag tttgaaaaag
    17581 ctgcagaata ccgagaccag atccgtgcca ttgagacaat catgactcgc caaaaaatga
    17641 caaatactga tttactggat cgagatgtat ttggttatgc gattgataaa ggatggatgt
    17701 gtgtccaagt gtttttcgta agacaaggaa agctaattga acgagatgtt tcaatgtttc
    17761 ctttctataa cgaagcagaa gaagatttct tgacttacct cggtcagttt tatcaggaaa
    17821 atgagcattt cattcctaaa gaagtcttga ttcctgatac gatcgacaag cccagtgtag
    17881 aagctttgtt agctacgaaa gtgctgcaac caaaacgagg agaaaagaaa aaattagttg
    17941 aattggcgag taagaatgcc tcagttgctt tgaatgaacg gtttgatctg attgccagaa
    18001 aacaggaacg gacgatcgga gcagttgaac gtttaggaaa cgcgatgaat atcccgaccc
    18061 caatacgtat cgagtctttt gataactcta atatcatggg gacgaatcct gtggctgcga
    18121 tggtcgtatt cattgatgga aaaccggcaa gaaaagaata tcggaaatat aaaataaaaa
    18181 cagtcaacgg accggatgat tatgcatcaa tgagagaagt catctatcgc agatattcgc
    18241 gagtgattcg cgaagggttg cctttacctg atttgatctt gatcgatgga ggaaaaggac
    18301 aagtagatgt ggcaaaagat gtcttagaaa atcaattagg aatcgatatt cctattgctg
    18361 gcatggcaaa aaacgataaa cataaaacaa atgagttgct attcggtaca gatttggctg
    18421 tagtaccgct tgagcgaaat tcaccagagt ttttcttact ccagcgaatc caagatgaag
    18481 tccatcgatt cgcaatcact ttccatcggc agctacgcag taaaaacagt tttgcttccc
    18541 gattagatga aattgaaggg ttaggaccaa aacgaaaaaa aatgctgctt aaagagttca
    18601 aatcgttgaa aaatatcaca gcagccacga cagaagaatt acaagaaatc ggactgccga
    18661 aaaatgtcgc acaaaacgta tttgataaat tacatcagga atgaggaaag ggcactttac
    18721 aaagaaagta aagtgtccta tcattttatg tacaacaaaa agagcgttta cgttttgttg
    18781 gtaaataata aatcgcttac gtctggtgtc taaggatata tgtgatttct tgaagaagga
    18841 gtgaagaaat gttagaagtc aaggatcttg ttaaaacttt tgggaactac acagctgtcg
    18901 atcatgtctc tttccaaatc cccgatggaa aaatcatggg gctaattggc caaaacgggg
    18961 ctgggaagac aacgactttt cgtttgatct tggatttttt gacacaagac caaggagaag
    19021 tgttatggaa tggccatcat cttggggaga aagattatga tgtcatcggt tatcttccgg
    19081 aggaacgggg actttatccg aaagtaacga ttcaagacca actgctttat tttgcagaac
    19141 ttagagggaa atcaagaaaa gagattgaac caaaaatcga cttttggatg gaaaaatttc
    19201 aggtaaaagg gaaaaagacg gataaagtaa aatctttgtc taaaggaaat cagcaaaaag
    19261 tccagctgat cgccacgtta atccatgaac ctaaattgat cattttggat gagccattca
    19321 gcgggttaga tccggtaaat gctgaattgc taaaagatgg aatattggaa ttgaaaaaac
    19381 atggttcatg tgtcattttt tctagccaca atatggataa tgtagaaaaa atctgcgatc
    19441 atttgatcat gttgcgaaac ggaaaaatgg ttttgaatgg aaaagtacat gagattcgtg
    19501 aatcatttgg ccgtacgaag ttattcttgg aatctggttt aagccaacag gaagtagaag
    19561 aaatcgaagg cgtgaaaaaa gtgatccttc atgaagacaa tacgttagag attaccttag
    19621 aagatcctga agctgggaaa gaaatcttta ctcgtgcgac tcagttcggc tacattccta
    19681 tgtttaacca gcaaccgcca actttggaag agattttcaa attgaaagca ggtgaaatcg
    19741 atgcgtaagt tttggattat taccaaagat gtctatttaa aaaatgttaa gtcgatttct
    19801 ttcttgatca tgatattagt cccttttatc atgctgggta tcatttatgt agcaggaaac
    19861 tttgctcaac aaaatagtga tgtcaataaa atcggcgtat tggctgataa tgagcaaatc
    19921 agtcagcaaa tgagccaagt caaaacagat gattttgagt tcaaagcagt tgattctgaa
    19981 gaaaaagcac aaaaagagct ttcggaagaa aaaatcgatg cgtatttagt agtctcaact
    20041 gagaatgata agataaatgg gatgctttat agcgaaaatt cattaggaca aagtacacaa
    20101 ctactgatac aacaacaact gactggcttg caatcgatgc ttcgagcgag tcaattaggc
    20161 atttcaccag aagaagtagc tagcttgagc caacaagcaa gtttgactcg acaaaaagtc
    20221 aattttgatt caaaaggaaa aatgacagtc ggagaagata actcgacagt ccaatacgtc
    20281 gtaagttatg tagcaacgat tgtcttgttc attattattt tgacgtatgc gcagatcatt
    20341 gcacaagaga tcgcttctga aaaaggcacg cggatcatgg aagtgatcct gtccagtacg
    20401 actgcacaaa agcattttta cggaaaatta acaggtgttt tacttgttgc attaacgcaa
    20461 atggcattat acggtattat ttttggtata ggatacagtc aattcaaaga tatggaaatg
    20521 gtcaaaaata tattagacgg tatctcattg gataacttga ttggaccatt cttgtggtat
    20581 tctcttctat tcatgatctt tggcattttg attttttctg ttttagcagc tttatgtggc
    20641 tctttggtca acaaagcaga agatacagca aaagcaattt tgccagtgac ttatctatct
    20701 ctaggcggat acatgcttgg attgatacta ggagcttcag atccaaacaa tatcgttatt
    20761 cggatcactt cgtatattcc attcttatct tcatatatta tgccggttcg cttagcaaat
    20821 gagacagtcg gtatgggtgg agcattgatt tccttgttgg tacttgtagc cgtaacattc
    20881 ttattgatga tcttgtctgc aaacatgtac aaatcgaatg tactcgttta tagcgaaggc
    20941 ggattatgga gttcattgaa acaatcgatt tcaattatga ggaatgaacg aaaaaaagca
    21001 taaaacaatc ctaaaataca gaaggaagct ggacatctat tgttcccagc ttctttttgt
    21061 atagacctat tttatttatt ttactatatt ggatatatat tcattgaaaa tagtgtataa
    21121 caactttata atattgagta taccaatttt tggaggcgtt ttaatggacc gttatttaga
    21181 tttactttca gaaaaattcc cctcaacgga ggatgtcatc acggaaatca tcaatctcga
    21241 agcaattttg cagcttccaa aaggaacaga gttatttttg agtgatatac atggcgaatt
    21301 tcctgctttt gatcatattc tgcgtatcgg ttcaggaaat cttaaagaaa agatcaaaga
    21361 ccttttttct gaacaaatga cggaaacgga tatcagccaa ttcactattt ttacggctta
    21421 tccagaatat gcattaacga ctgattggta caaagaacaa gataaatcgc agctgatcca
    21481 tcatttgatc gatcttttac ggtttacgac agtcaaatat acccggtcaa aagttcgaaa
    21541 aggattgccg aaagaatata gctatatcat tgaggaactc ttgtatatcg atgaccgtat
    21601 caatggaaaa aaagactatg taaaaaaaat cattgaacaa cttgttttga tgaaggaaga
    21661 agaaaaattt ttaacgaaac ttgcacagac gatccaaaaa tctgtcatcg atcatttgca
    21721 tatcgttgga gatatttttg accgaggaac tcaagcagcc aaagtgatgg atcgaatcat
    21781 ggatttttct tctgtagata tccaatgggg aaatcatgat attttgtgga ttggtgcgta
    21841 ttgcggctct gaggcatgtt tactgacact tttacggatt gcagcacgct ataattattt
    21901 atttgaatta gagaaagaat atgggttgaa tttacgtcca ttgttttctt ttgctcatca
    21961 aacttatcag aagaatccag tatttgcgcc taaaaacagt aaaaatctat ccgaaagaga
    22021 gcaagaagaa ttagaaaaaa tccatcaggc actttctatc cttcaattca aatcagaaca
    22081 tcagttattg gatcgtcgac ctgaatttaa aatggacgat cgtaaattgc tggaaaaaat
    22141 cgactatgaa gcatcaacga ttgatttaga aggtcagctc tatgagttga aaggaacttg
    22201 ttttcaaacg atccagccag atgatccaaa aaaattcttg gaagaggaac aaaaagtgat
    22261 tgatagtttg atgtattcat tccagcactc tttgagaatg caaaaacata tgacgttttt
    22321 gatcgaaaaa gggggaatgt atctcacgaa caatcagcat gtgctgtttc atggctgtat
    22381 acctgtagac gagaagggac agctcttagc atttgaatta gaccaatctt atcgcggaaa
    22441 agaactgctg ggcttttttg aaaaacagat aactgaaagc gcaaaagatc tgactgcaaa
    22501 agaagattta gcaaccgatt tgatttggta tgcgtggagt gggccatttt cgcctttatt
    22561 cggcaagagt aaaatggcga cttttgaacg ttattttctc acagagcctc atacgcacgt
    22621 cgagaaaagc aatccttatt atcacttgcg agatgaagag tggttcagtg aaaaaatttt
    22681 agcagaattc gacacaaaag aagaagggtc agcaatcatt aatgggcaca cgccagtaaa
    22741 agtaaaaaaa ggggagtcac cgatcaaagc aaatggaaaa gtatttgtca ttgacggagg
    22801 tttatccaaa gcttatcaaa aaacgacagg aattgccggt tattcactat tatgcaattc
    22861 ttatggtttc caaatcgtga cacattatcc gtttttgcct attgaacaac aatttgcaaa
    22921 aaaatccgac caaacaaatg tgaaaaaagt aatcgaacag caactaccac gcaaactcaa
    22981 tcgtgataca acaaaaggta tggaactgca gcagcagatt acccagctca aaaggctgtt
    23041 ggactatcga aaggacgatt gagaagaatt ccagtgaaca ttgttccata gactggagac
    23101 tgagacaaaa gtcgtgtcgc agtctctttt tccggataaa cggtgttctt ctgccaactc
    23161 ctcggtcaca agtcacacta ttcaacaaac atgaaaagct gttttttgaa attgctcctt
    23221 gtgactcaga gttgaacggc aggctcacga cctcttggta taaacggtgt tcaaaagcca
    23281 gtttctcggt attaagccaa atttatgcaa aaatgtgaaa agccattttc acaaaatttt
    23341 tcttaatact cgaaactaaa cagctttttc acaaccccct cagaaagtct attaaaattc
    23401 attcacaaag aaatcgaata agcgctgttc agatggatgt acacggtggg taagttccgg
    23461 atgccattga acaccgagga ttttatgcat atcatctttg ctttgaaccg cttcaatgag
    23521 tccatcgtta gaaaaagcga tggcttttaa taaaggtgaa agatctttga ctgcctgatg
    23581 atggtaagaa ttcacaaaat atggatcggg gagaaggttg cttaacaaac tgtctgaaac
    23641 aatcttgact tcgtgagtcg taaattgcgg ggcagtgggc tgttgttcat gttttactga
    23701 ccactttgga tataatgaaa gatcttgata aagtgtacct tccaaaacga cattcaatag
    23761 ttggagtccc cggcagactg caaaaattgg ttttctttgt ttcaatgctt ccaagatcag
    23821 tgcctgttca aattggtctc gttgaatatt tgtttccgtt agctttggat gaggatcttc
    23881 catgtaaagt tgtggactga catcttgacc acctgcaaga ataagtttgt cgatttgtcc
    23941 aatcagttga ggagctgaat ctggagacat gactggcaga ataagaggca atccttgagc
    24001 atctaaaact gcatccacga atccttgcgg tgtatagctc acttgatttc cttggaaagt
    24061 atctgttgct cgaatcagtt gatttccggc aatgccgatg attggtttca tttgtattcc
    24121 ccctaaagta gacttattgt aacaaggatt taaaaaaaat taaactatgt aagacttgcc
    24181 cctatactta tctgttcttg ctgtgttact atctgtaaag agaaatttcg tgtgaaattc
    24241 atgacgatag ttgaaaatat tgcttaattt gttataataa aaagtaagca gaaataaaaa
    24301 ggttgggcgt aagcttcaag gatctagttc ttcaaggggt gagaaggaca ttttattgaa
    24361 ggggcctgcc tatactaagt tatctttaga ggtacatact ttcaatgaaa aaagaaagca
    24421 tgtacctctt ttttaaatcg aaaggaagtg aagcaagtgt tagaagcttt ggaacaaatc
    24481 cgaaaagcag aagaagaaaa tgaaaagcgt gctcaaacac tgcggcagca gctgaagatc
    24541 tatgcaacag aaaaagagca ggaattggaa acgatccaaa caaactatcg acaacaacta
    24601 aaacagttgg tggaagaaaa agaacaattt gaaatacaag aattagaaaa agaaaaaatg
    24661 aaattgaaag cagaagcaga caaggtgcaa gcaactttga aagatcaata tcaaaagcat
    24721 cagagtcaag cgatcggtgc gatcattgaa agggtgaaag aaacttatgg ccgtcactaa
    24781 gatggaaaaa gtgaccctca tttcagataa aagaaatcag gagaggattt tacaagcagt
    24841 acaaggaatc caaaacgttg aaatccggga tctgtatcaa aatagaacaa acaaccagtg
    24901 ggtggagaag tattttccag ataccacgat tattgataaa gaagcaaaat taagcgcttt
    24961 aggtaatcgg ttgaaagaca tccaagaagc tatccaattc atacaacacc acggcgatag
    25021 ccgtcaaaag aaactccatt tgaaacgtca agaactttcc ttacaagatt tggaagccaa
    25081 ttattccgaa gaaaatttca taaataaatt gaaagaagtc cttgatttaa aagaaaagtg
    25141 ggaacaacta actgaagaac gtgatcgttt agaagaagag gaagaatggc ttcttcattg
    25201 gcaacagctg gatattgctc caagtatgta tgcaagtaag acgaccattt ttgagatggg
    25261 gactgtgagt gcagcaaatt ttgaaagttt caaggaagaa ctgagtaagc ttgatgacgt
    25321 ttatatggaa gaaatagatt atacgccaaa acaagtttat ctttcgtatg ttgctttaaa
    25381 aaaatcagtt gctgttatag aagaaattgc tgggaaatac gggttcaata aagaaacgta
    25441 tccttatgaa caaaatccta aagaaaaact gcaagagacc aagcagcaat tatccattgt
    25501 tcacgagcag caaaagaaga taacgacagc gttaggtcag tgcagtggct atatccaaga
    25561 atttgaatgg gtagaagaag tcacacttgc cgttgcagaa cgagaaaaaa taaaagaacg
    25621 gtttatccat gcgacttatc tgatcgtttt gcaaggttgg gtagatgcag aagaaaaaca
    25681 gaatttgctg catgtgttaa acgaaaccgt atcagaagaa gatatatacg tcagtttcga
    25741 aacaccaaac tcagaagaga ttcagcagga agtccctact aaattgaaaa accatccgct
    25801 tgtcgaacct tttgaattgc tgactgaaat gtacagtctg ccaaaatatg aagaagtaga
    25861 tccaacacct tggatgacac cattttatct tgtcttcttc gggatgatgg tcgcagatgt
    25921 tggttatggt ttgctgatgc tcattgggac aatactagct caaaaactac ttgttctacc
    25981 acgcgggatg aaacgtttcg taaaattctt tgagattctc tcgataccat cgatcatctg
    26041 gggattgatc tacagttcat ttttcggaca gtcattacca aaagaactgt ttggcatccg
    26101 cttgcctttc ccaatcctat ctacgacgga tgacgtcaat acgattttga ttttatccgt
    26161 gattttcggt ctgatacaga ttttggttgg attatttgtg gcagcaaagg aaaatatcag
    26221 gagaaaagat tacttgagtg ctgtcagtga tggatttgcc tggcaaggga tcctaattgg
    26281 gatcgttatt gcgttggttg gtgccatgtt gataaaaaat acagcatttg tttaccttgg
    26341 cgctgggcta tcgatcattg ctgccttatg tatcttgatc gttccgatta tccaaacgcc
    26401 gtctaaagtc aaaggtgctg ccaaaggtgc gtataatctt tacggactga ccggctatat
    26461 cggtgattta gtcagttata cacgtttgat ggcattgggt atttcaggcg gaagtattgg
    26521 agcggcattc aatatgctag ttgctttcat gccgccagct gctcgattca gtgtagggat
    26581 ccttttgatc atcgctttac acgcactgaa tatgtttttg acactgctaa gtgcctatgt
    26641 ccatggcgca cgattgcagt atgtcgaatt ttttgggaag ttttatacag gaggcggacg
    26701 tgcctttgat ccgctgaaaa cggcggaaaa atacgtcaac atcaatcaca agaaaaaaaa
    26761 caataaataa ttggaggatt tttatataat gatggattac ttaattagtc aaaatggtgg
    26821 aatggtattt gcggtattag cgatggcaac agcgacgatt ttttcaggga tcggttcagc
    26881 taaaggtgtt ggaatgacag gtgaggcagc agcagcattg acaacaagtc aacccgaaaa
    26941 attcgggcag gctctgattc tgcaattgct tccaggtacg caaggtttgt atggattcgt
    27001 tattgccttt ttgattttta tcaacttagg aaatgacatg tcagttgtac aaggactgaa
    27061 ctttctaggt gcttctctgc cgattgcttt tacaggcttg ttttcaggta ttgcccaagg
    27121 aaaagtcgca gcggcaggta ttcaaatttt agcgaaaaaa cctgaacatg ctacaaaagg
    27181 aatcattttt gctgcgatgg ttgaaacata cgcgatttta ggtttcgtta tttccttcct
    27241 attagtgttg aatgcctaag cataaaggag gaaaagcaga tgaacgccat tgaaaaaatc
    27301 atctctcaga tgaacgaagc agcagagcag gaaagagcgg cgttagaaca agaagagcga
    27361 atgaagatcg atcaaaactt tgaacaaaaa cgtacgcaag ttgaaacaga acatcaaaaa
    27421 caaaaagaaa aacaaatcga attactcgaa aaaaaatacc gtcagctaag gaatcgccag
    27481 caagtagaag tacgacaaga aaatttgaat gcgaagcagg aatttttaag acgattgttt
    27541 gcagatgcgg taactgaaat ggaaaattgg gatgaatctg aacaaatcca gtttatcaaa
    27601 aatgcattgt attcattacc tttgacagga aaagtagcat tcattgctgg agaaaaatca
    27661 gcagcctatc tttctcagac gttgttggat gaatggaaca atgaacttcc ctttatgatg
    27721 gttttaagtg atgaaactgt tgctgatcaa gcgggttttc tgataaacga tcaaggagtt
    27781 cagtataatt ttcttttcag cagccttgta caagatatcc aaggaacgat gagttttgag
    27841 attgccaatc aattattcga ataaggaggg atcagtgtat ggaataccac gaactaaatc
    27901 ccttgattcg cggaagagag cttgaacttt tgtccaatga gacatttgaa cggatgattc
    27961 aaacaaattc tgtcgaagca cttggtgaaa tattgaaatc cacgatttac agtccatata
    28021 tttatgacgg atatgaatat gacttcgaac agaatctttt aaaggagcag agccgtctat
    28081 ttcgctggtt aaaggaatca gcaccggagc ctgaaatcgt ttggatctat acgatgcgtt
    28141 atactttcca taatctgaag gttttgacaa aagctgagat gacaaatcaa aatctggacc
    28201 atttgtatat cgacgatgga ttttattcat tagaaacctt gaaggatgcc attcatacgc
    28261 aagtttcaaa tgaacttcct gaaagcatta tggacagtat acgagaagta cgtgattatt
    28321 ttgaagagtc taccattctt cagggaattg acgtgattta tgatcgacag tttttgacag
    28381 agcagcgacg gcttgccgat aagctaggat atcaagaatt gctgacagaa gtcattgctt
    28441 ttattgactt gaccaatatc acaacgatgg ctcgaggaat attgcagaaa agaacatatg
    28501 ggttcatgac gacggtactt tccagctcgg gaagtatcga aaaagaagta tttttgaact
    28561 ttgtagataa agatatggaa gattacacga attttctatt agagacagat tatgctgatt
    28621 ttctgcgccc tgtcatccgt gagggaaata ttgatctcgt aaagcttgaa cagttgaaag
    28681 atgattatct aagttcattg tatcaagaag cacaaaccca ggctttcgga ccactgccac
    28741 tactagcctt tttgaacgca aaagaggtag aaagcaaaaa ccttcgttta ctggttatcg
    28801 gcaaaagaag ccatttcgaa tcagaaacaa tcagaaagcg ggtgagacaa gtctatgacg
    28861 cataagatcg gtgtagttgg cgacaaagat tctgttttgc cattcaaatt gtttggcttt
    28921 gatgttcgtt atggtacaac caagcaaacc gttcgtcagg caattgaaga aatggcaaag
    28981 aatgagtatg gcgtgattta tgtcacagag caatgtgcag cattgagccc agaaacgatc
    29041 gaacgttaca aggaacaaat gatacctgct atcgtattga ttcccaatca tcaaggatca
    29101 ttagggatag ggctggaaga aattcaaaaa aacgtagaaa aagcagtagg tcaaaatatc
    29161 ttatagtatg aaaaggagat agaaattttg caaattggaa aaatcataaa agtctccgga
    29221 cctcttgtaa tggctgagaa catgtctgat gccagtatcc aagacatgtg tctagtggga
    29281 gatctaggag ttatcggaga aattatcgag atgcgtggcg atgtggcgtc tattcaagta
    29341 tatgaagaga cttctggaat tggtccaggc gaaccggttc gctctactgg tgaagcttta
    29401 tctgttgaat taggaccagg gatcatttca caaatgtttg acggaatcca acggccatta
    29461 gataccttta tggaaatcac acaaagtaat ttcttagggc gcggagttca attgcccgca
    29521 ttggaccatg agaaaaaatg gtggttcgaa ccaacagtag aagccggaga aacggtgagt
    29581 gctggagatg ttatcgggat cgtagaagaa acaaaagtta taaaacataa aatcatggtg
    29641 ccaaatggga taaaaggaac gatcaaaaca atcgaaaaag gttcattcac tattgatgaa
    29701 acagtatgtg tcattgaaac cgaaaatggt gacaaaaatt tgacgatgct tcaaaaatgg
    29761 ccagtaagac gtagtcggcc aatcaaagaa aaattaaacc cggatgcacc catgatcact
    29821 ggacagcgag tgattgatac attcttccca gtgacaaaag gaggagcagc agctgtacct
    29881 ggtccttttg gtgcagggaa aacagtcgtt cagcatcaaa ttgcaaaatg ggccgatgtg
    29941 gacatggttg tctatgtggg atgtggcgag cgtggaaatg agatgacgga tgttttaaat
    30001 gaattccctg aattgattga ccctaataca ggagaatcgt tgatggaacg tacggtattg
    30061 attgctaata catcaaacat gccagtagca gctcgagaag cttctatcta tacgggaatc
    30121 acaattgcgg aatatttccg tgacatgggt tatgacgttg ctatcatggc agactccact
    30181 tcacgatggg cagaagcact tcgtgagatg agcggacgtt tggaagaaat gcctggtgat
    30241 gaaggttatc cagcttatct tggttcacgg cttgcggaat actatgaacg atctggaaga
    30301 gtcattgcat taggatcaga aggtcgcgaa ggaagtataa ccgcaatcag tgctgtatcc
    30361 ccgtctggag gggatatttc ggaaccagtc acacaaaata cattgcgggt cgtaaaagta
    30421 ttctggggtc ttgattccag tttagcccaa aaaagacatt ttccttcgat cgattggata
    30481 caaagctatt ctctttattc aacagaagta gggaagtata tggatcatat ccttcaagct
    30541 gactgggcat ccatggtcac cgagggcatg cgtattttgc aggaagaaca gcagctgaat
    30601 gaaatcgtgc gtttggtagg gattgattcc ctttcagata atgaccgtct gacattagaa
    30661 gtggcaaaat ccattcgtga agattatttg cagcaaaatg cttttgatga tgtcgataca
    30721 ttcacttcaa gagagaaaca attcaaaatg ttgaagatga tcttgacctt tggtgaagaa
    30781 ggacgccgtg cgttgtcgtt aggtgcttat ttaagtgaga tcatggacgg taccgtatcg
    30841 attcgtgaaa ggatcggacg aagcaaatat atcccggaaa cagaattaga aaaactagat
    30901 caaattattg gtgaaatcaa gcaaacgatg caaacgatcg tttcagaagg aggcatgagc
    30961 aatgattaaa gaatatcgta cgatcggtga agtcgtcggt ccattgatgg cggttgaaaa
    31021 agttgctggt gtcaaatatg aagaattaat cgaagtccgt atgcaaaatg gcgagattcg
    31081 tcgtggacaa gtattggaag tccaagaaga taaagcaatg gtccaaatat tcgaaggaac
    31141 aagtggtatc aatttacgtg actcttctgt acgattttta ggccatcctt tagaactagg
    31201 tgtatcggaa gacatgatcg gtcgagtatt cgatggtttg ggacgaccga aagataacgg
    31261 accagagatc ctgcctgaaa agcatttgga tattaacgga gaagtaatca atccagtagc
    31321 tcgggattat ccagatgagt ttatccaaac aggtatctct gcaatcgatc atttgaatac
    31381 attagttcgt gggcaaaaat taccagtatt ttctggctct ggtcttcctc ataaagaatt
    31441 agctgctcag atcgcgcgac aagcaacggt actcgattca tcagatgatt tcgccgtagt
    31501 ctttgctgct attggaatca cttttgaaga agctgaattt ttcatggaag attttcgtca
    31561 aacgggcgca atcgaccgtt ctgtcatgtt tatgaattta gcaaacgacc ctgcaatcga
    31621 acgtatcgct actcctcgta tggctttgac tgctgcagaa tatcttgctt atgaaaaggg
    31681 aatgcatgtt ttggttatca tgacagacat gaccaactat gcagaggcat tgcgagagat
    31741 ttctgcagca cggcgagaag ttccaggaag aagaggatat cctgggtatc tttacacgaa
    31801 cttagctact ttattcgaac gagcaggaag aatccgcgga ctaaaaggat cggttactca
    31861 aattccgatt ctaacaatgc cagaagacga taaaactcat ccaatccctg acttgactgg
    31921 ttatattaca gaaggacaga tcatcttgac tagagagctt tacaagagtg ggattcagcc
    31981 accaattgat gtactcccgt ctctttcgcg gttaaaagat aagggaactg gacccggaaa
    32041 gacaagagaa gaccatgcag ctacgatgaa tcagcttttt gcagcctatg cacaaggaaa
    32101 acaggcaaaa gaattagccg tagttttagg tgaatctgct ttatctgatg tagacaagat
    32161 ctatgcgaag tttgctgagc ggtttgaaaa agaatatgtc aatcaaggat tctacacgaa
    32221 ccgttcgatt actgaaacac ttgatctagg gtgggaactg ctatcgatgt tgccaagaac
    32281 cgaattaaaa cgaatcaaag acgacatgct ggacaaatat ctgccagaag ggaagtgagt
    32341 tctgatggca cgattgaatg tgaatccgac ccgcatggaa ttgactcgat tgaaaaaaca
    32401 gttgacaact gccacaagag ggcataaatt attaaaagat aagcaagatg aactgatgcg
    32461 tcaattcatc ctgttgattc gaaagaacaa ccagctgcgt caagaaatgg aagagtctat
    32521 gacagaagca atgtctgatt ttgtattggc aaatgcttcg atcacggaac ctttcatcga
    32581 agaattattt gttttgccgg cggaaaacgt tgatttgtct atcgtagaca aaaatatcat
    32641 gagtgtaaaa gtacctgtga tgaattttga atatgatgaa cagctgacag acacaccttt
    32701 ggaatacggg tatttgaatt ccagtgccga actggatcgg tcaatcgatc ggtttactac
    32761 cctgcttcca aaacttttgg aactgacaga aatcgaaaaa acttgtcaac tgatggcgag
    32821 tgaaatcgaa aagacacgtc ggcgagtcaa tgcattagaa tatatgacga tccctcaatt
    32881 agaagaaacg atctattata ttcgtatgaa gctggaagaa aacgagcgcg cagaagtaac
    32941 aaggttgatc aaagtaaaaa atatgggtac cactgactaa gtggtatctt tttttaaaag
    33001 aggaaaacga tgattaaaca aagcaaacaa ataaagaaac gtttgccggc tgttcagctt
    33061 ctcgcattag gattttttct cctgatttta gttggcggcg gcctacttac cctaccattt
    33121 tttagtaata gtgggcatgg gacaaatttt atcgatgcgc ttttcactgc aacttctgct
    33181 gtctgtgtaa cgggattgac aacattgaat acagcagagc attggaacag tgctggtcaa
    33241 tttttgatca tgcttttgat tgagatcggc ggtcttggtt ttatgatggt tccgattcta
    33301 tgctttgcat tgataaagaa gaaagtcagt ctcagcacac gtattatatt aaaagaagcg
    33361 ctgaatctag aagaagtatc aggtgtgatg aagctcatgc tttacatcct aaaattggcc
    33421 tttgtgatcc aattgattgg tacggttgct ttatcttttg tatttgtgcc tgaatttggc
    33481 tggttgaaag gtatctggta cagtttgttc catgcaatct ctagtttttg taatgcaggg
    33541 tttgatttgt taggagacag tttagccaat caccagcaaa atgtttattt gatcatggtg
    33601 gtatctgctt tgatcatttc tggtgggtta ggcttcatcg tatggcgtga tttactggaa
    33661 tatcatcaga aaaagaagat gacgattcat tcaaaaatcg cattaaccgt tacaggtacc
    33721 cttttattag gcggattcgt tgtgttcttt ttcacagagc gcaatggggc aacgctagtg
    33781 gaagggaata tgtttcaacg attggcaaac acctttttta tgagtgtgac acctagaacg
    33841 gctggatatt attcagttga ttacctccat atgagccatg caggtctgat tttaacgatg
    33901 tttttgatgt acatcggcgg aacatctggg tcgacggccg gcggattgaa aacaactacg
    33961 ttaggtgtgt tgctgatcca gatgcgttcg atgttgaaag gacgtacgag agcggaagca
    34021 tttggccgta ccatccgaca aggcgcagtg ttgcgggcac ttactttatt ttttgttacg
    34081 ttgacgttgt gtgtagtcgc catcatgatt ttatcggtga ctgaaacgat acctaaaact
    34141 tctggcatcg aatatattgc ttttgaggtc ttctctgcgt ttggtacagt aggtcttacg
    34201 atgggattga cgccagactt gactgcaata gggaaattga tcattatagc gcttatgtac
    34261 attggacgag ttggtgtatt gactgtggtc ttctctctga tgacaaaagc acaaagaaca
    34321 caatcaaaat acaaatatcc agaagaaagt gtcatgattg gataatttca tcttctgaca
    34381 gaaacctagt gatttagtaa aataagaaaa taaaataatt tttcttgctt cttggatact
    34441 ttaagtgcaa aatgttcgtt tattttttga ggcactaccc gttgttgaac ctcgggtagt
    34501 gcctcttttt tattgaaagg aatcaataag atttgattcc tttatttatc aataggttat
    34561 aattcatatt tttttatttt ttcttttacg atacttagtc cagaagctaa aatttcaagg
    34621 ttattagagg atagggaaat tcgaacgaat gcatctggtt gcgtacgtgt agtaaagcgt
    34681 ctggaatgat aaagtcggat accgttagca agaaagtcat tctcaattat cttagggtca
    34741 atctcaggat caactggaag aattcgatag taagggacac gattcgttgg tttttctact
    34801 ttgaaaagag aatcaaaaag ctgattggct ctttttgtaa gacttaattt agtttctaag
    34861 atcctatttg cttcatttga atggaggatt tgagtgatga cttccacatc taatccagaa
    34921 gttttcacat ttatattaaa aattgcttgt tctatcttgc tttggacatg tactggaact
    34981 acaagataag caactcgcaa tccagaacaa ataaattttg tcattccagc taagtagatt
    35041 gtttggttag gtaataaatt ttggaaagga ggaatgatcg tatgttgata gtaagttgtt
    35101 aaaaatgaat ggatatcatc ttcaataacc catagacctt ctttttcaat cacttcggct
    35161 aattgctctc gtcttttgtg agagatttga aaccctatag gattattaca agaaggcata
    35221 agaaagacac catgaatctt cttttttcta cattcctgaa tcaatatatc aggtcgcatt
    35281 ccttgctcgt caaaatcaat tggaacgatc tctaaatgat ataactgagc caattcaatg
    35341 aaattagcgt atgtatatcg gtctgttgct attctatcac ctggagagaa aagtgcagaa
    35401 agtgtgatag ccaatccatt ttgaacaccg gaaacaatag atagagattc taaggacgga
    35461 atgactcctt gagtttttaa ccaatttgct gcagtagtca attgaaaatt tgtgcctttg
    35521 ggatcttggt aattaagcaa accaacaaga tttgggttat tactagttga ttgaataaac
    35581 gggacaacga atttattaca ttcttcaaaa gaactaacca gaccaaaatc aatgatatgt
    35641 tcggttactt cattggtaga gatggtgatg gactcaaaga cattgggaga aataaatgtg
    35701 ccacttccaa tattcgtata cagcaaacct ttctcaattc catatttata agccttgcca
    35761 acggtagtaa aatttaaatc taaataatca gctagctctc tttgggaggg taactgagtg
    35821 tttttctgta atttattctt taaaatatct tgttctaatt gttgaatcaa cgatcgataa
    35881 taaggacgct tcaatttttg tttgtctggt cgccaagtta acgggtaatt gtcataagaa
    35941 ttaataggca ttttctccac tccttaaaat tgtataccat acaattttac c
//
LOCUS       DANKHL010000004        33585 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000004 DANKHL010000000
VERSION     DANKHL010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.004"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..1152
                     /locus_tag="PW101_000138"
     CDS             <1..1152
                     /locus_tag="PW101_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDL2512572.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="PW101_000139"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="PW101_000139"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDL2512573.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="PW101_000140"
     CDS             2728..3003
                     /locus_tag="PW101_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDL2512574.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="PW101_000141"
     CDS             3009..3776
                     /locus_tag="PW101_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HDL2512575.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="PW101_000142"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="PW101_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDL2512576.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7793
                     /gene="recQ"
                     /locus_tag="PW101_000143"
                     /pseudo
     CDS             6034..7793
                     /gene="recQ"
                     /locus_tag="PW101_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
     gene            7907..8209
                     /locus_tag="PW101_000144"
     CDS             7907..8209
                     /locus_tag="PW101_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDL2512577.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8212..9375
                     /locus_tag="PW101_000145"
     CDS             8212..9375
                     /locus_tag="PW101_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL2512578.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9401..12829
                     /locus_tag="PW101_000146"
     CDS             9401..12829
                     /locus_tag="PW101_000146"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDL2512579.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12850..13974
                     /locus_tag="PW101_000147"
     CDS             12850..13974
                     /locus_tag="PW101_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512580.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13964..14401
                     /locus_tag="PW101_000148"
     CDS             13964..14401
                     /locus_tag="PW101_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2512581.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14385..14723
                     /locus_tag="PW101_000149"
     CDS             14385..14723
                     /locus_tag="PW101_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDL2512582.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14842..15402
                     /gene="rsmD"
                     /locus_tag="PW101_000150"
     CDS             14842..15402
                     /gene="rsmD"
                     /locus_tag="PW101_000150"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDL2512583.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15399..15890
                     /gene="coaD"
                     /locus_tag="PW101_000151"
     CDS             15399..15890
                     /gene="coaD"
                     /locus_tag="PW101_000151"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDL2512584.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15880..16923
                     /locus_tag="PW101_000152"
     CDS             15880..16923
                     /locus_tag="PW101_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDL2512585.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17082..17810
                     /locus_tag="PW101_000153"
     CDS             17082..17810
                     /locus_tag="PW101_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDL2512586.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17926..18171
                     /locus_tag="PW101_000154"
     CDS             17926..18171
                     /locus_tag="PW101_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2512587.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18168..18386
                     /locus_tag="PW101_000155"
     CDS             18168..18386
                     /locus_tag="PW101_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDL2512588.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18522..19061
                     /locus_tag="PW101_000156"
     CDS             18522..19061
                     /locus_tag="PW101_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDL2512589.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19054..21327
                     /locus_tag="PW101_000157"
     CDS             19054..21327
                     /locus_tag="PW101_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDL2512590.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21365..22411
                     /gene="holA"
                     /locus_tag="PW101_000158"
     CDS             21365..22411
                     /gene="holA"
                     /locus_tag="PW101_000158"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDL2512591.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22500..22754)
                     /locus_tag="PW101_000159"
     CDS             complement(22500..22754)
                     /locus_tag="PW101_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDL2512592.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23154..23423)
                     /locus_tag="PW101_000160"
     CDS             complement(23154..23423)
                     /locus_tag="PW101_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512593.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23893..24348)
                     /locus_tag="PW101_000161"
     CDS             complement(23893..24348)
                     /locus_tag="PW101_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDL2512594.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24486..26414)
                     /locus_tag="PW101_000162"
     CDS             complement(24486..26414)
                     /locus_tag="PW101_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL2512595.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26611..27444)
                     /locus_tag="PW101_000163"
     CDS             complement(26611..27444)
                     /locus_tag="PW101_000163"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDL2512596.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27636..28340)
                     /locus_tag="PW101_000164"
     CDS             complement(27636..28340)
                     /locus_tag="PW101_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2512597.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28363..28989)
                     /locus_tag="PW101_000165"
     CDS             complement(28363..28989)
                     /locus_tag="PW101_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2512598.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29315..31690
                     /locus_tag="PW101_000166"
     CDS             29315..31690
                     /locus_tag="PW101_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDL2512599.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31714..32205)
                     /locus_tag="PW101_000167"
     CDS             complement(31714..32205)
                     /locus_tag="PW101_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDL2512600.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32392..33312
                     /locus_tag="PW101_000168"
     CDS             32392..33312
                     /locus_tag="PW101_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2512601.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33466..>33585
                     /locus_tag="PW101_000169"
     CDS             33466..>33585
                     /locus_tag="PW101_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF59 domain-containing protein"
                     /protein_id="HDL2512602.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLG"
BASE COUNT        11119 a         5880 c         6969 g         9617 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt atccagcagt cccaaagaga agaagggcaa aagcaaaaag
     7081 aatatgaaaa attaaaagcg atgacagaat acgtgtatat tgagtcctgt cttcagcaat
     7141 acatcctaaa ctattttggc gagacaagca gtccgtgcaa ccgttgtggt aactgtttag
     7201 atgacagaga aatagtagaa gtaacgacag aagcacagat ggttctttct tgcttaaaac
     7261 gtatgggtga gaactatggt aaacaaatgc tgatgaaagt cttagcagga tcgaaagaac
     7321 aaaagctgca agctttaggt tttggacatt tatctaccta tggactgatg aaaaaccaat
     7381 cgcaaaaaga aacgatgcag ttaattgaat atttgatatc gaatggctat cttctgacca
     7441 tcaatggtga gtatcctgtt ttgaaagtaa cagaaagagg gatacaagta ttgaaaggac
     7501 aagaatctgt ttatcgaaag gaacccaaga aagtccagca gctatcggat gaagaaaccg
     7561 atacactatt tgaagtgctg cgggaactgc gaacggatct agcatcagaa gcaggcgttc
     7621 ccccgtatgt cgtattttca gattccacat tgaaagaaat gagccgaata cgtccatcaa
     7681 gcagattgga gatgcttcag ataaaaggag ttggccaaag caagcttgat aaatatggcg
     7741 aagccttttt atctcggata aaaaatgcgg actctaacgt tctggataag tagaaagaac
     7801 gaatacgtaa aaatgaataa aaacaactgg ttaaatctgt tttttttgct ccttatgaaa
     7861 atcaaatatg ttataattaa ggaattagaa tgggaggtaa gcacagatgg attctttgtc
     7921 gaaaaaactt gcgatccaag tattaaatga tgaagcagaa cggatcaaac gattaatccg
     7981 aaatcaaaac aacagtctct gtatctcaca atgcaaagcc ttcgaagaag tggtagatac
     8041 acagatgtac ggcttttccc ggcaagtcgg ttttgctgtc cgtataggag taatcggaaa
     8101 gaacgagggc cagctgatgt taagtgagct agaacgagaa ttgaatcagt tatatagtga
     8161 ggtttaccaa gaaatttatg ataaaaaaga agttggcaag gaggaataga cttgcctaag
     8221 aaagtgaaaa aaaggcatct attagactat agcatcttga ttccctattt aattttatgt
     8281 gtattgggat tgatcatggt atatagttct acgtcgtatc tattattaga aaacggacaa
     8341 aatccgtccg catcagtcat taaccagagt attttttggg tgcttagctt gattgctatc
     8401 gcactacttt ataaaatgaa aacagatgtt ttgaaaaacc agcgtttgat catggcagcc
     8461 atcgcagttt tgacgatttt gctgctgatt gttgtgtttt ttggtaagga gatcaatggg
     8521 gcgaaaggtt ggctgcaaat tgcaggattc tcgatccaac ctgccgaata cctaaaaatc
     8581 atttctatct ggtacctgtc tctgacttta tctaaaagac aaaacagtgt acaaaaagat
     8641 tttcttggga ctgtgaaacg tccacttgca atggttatcg gacttactgc attagtcgct
     8701 attcttcccg attttggtaa tgcggcagtt atctttttga ttattttggt tctgctttta
     8761 gccagcggag tcaattatgt gtatactttg attgtaggag taggaggctt ttgtctaagt
     8821 acttttacga tctggctgat caatatcaca aacggaaaga tcttacccgg acgtttacag
     8881 tatatctata atcgatttgc tatttaccaa aatcctttct cagacgaatt aaataaaggg
     8941 catcaactag tcaacggcta ctatgccatg ttcaacggag gactatttgg caggggatta
     9001 ggtaacagta tccagaaaaa aggattttta caagaagcac aaaccgattt tatttatgca
     9061 atcgtagtgg aagaactggg agtgattatg gggattttga tattagcgtt gctattcttt
     9121 atgattgtgc gcattgtcct tgtaggtatc cgttcaaaag atccgttcaa ttcgcttttg
     9181 tgcattggga tcggcgcaat gtttttgatc caagtatttg tcaatcttgg aggaattact
     9241 ggcgttattc ctttgacagg gatcacgttc ccatttttaa gtcaaggagg ctccagtttg
     9301 ctgatgcttt cgatttgtgt cggctttgtc ctcaacatca gtgcggatga aaaacgcaag
     9361 agcttaggtt tataataaaa aaagatgtag gagtggttcg atgaaaaaag ttttagttgc
     9421 taacaggggt gaaatcgctg tacgtgtatt tcgtgcttgt acagaattag gaattaaaac
     9481 tgttggaatc tatgcagaag aagatgaata ttccgtacac cgtttcaaag cggatgaagc
     9541 atatttagta ggacaaggaa aaaaaccgat tgatgcgtat ctagatattg aagggatcat
     9601 ttctatcgcg aaagaatgcg gagctgacgc tattcatccg ggttacggtc ttttgtcgga
     9661 aaacttgaat tttgcgcaac ggtgcgaaga agaaggcatc atttttgttg gacccaaact
     9721 gcatcattta gatatttttg gagataaaat caaagcaaaa gctgcagcta tcgaagcggg
     9781 gattgcttcg attccaggca cagatggacc aatcgcttct atagacgatg ccttggagtt
     9841 tgcgaaacaa tacggttatc cgattatgat caaggcagct ctaggcggcg gcggacgcgg
     9901 catgcgtgta gctcacgatg aaaagagtgc aagagagggc tacgaacgtg cgaaaagcga
     9961 agcaaaagca gcctttggta gtgatgaagt gtatgttgaa aaatacatcg ctaatcctaa
    10021 gcatatcgaa gtacagattc tcggggatac gcatggaaac gtcatccatc tttttgaaag
    10081 agattgttct gttcagcgcc gtcaccaaaa agtagtagaa gtagcgcctt gtgtctctat
    10141 gaatgaacag caacgtcaaa aaatctgcca ggcagctgtc caattgatga aatatgtcgg
    10201 gtatgtcaac gcaggtacag tagaatttct tgttgaagga gatgattttt actttatcga
    10261 agtcaaccct cgcgtccaag tggaacatac gatcacagaa atgattacag atattgatat
    10321 cgtaacaacg caactgttga ttgctcaagg tttggatctg cataaagaaa tcggattgcc
    10381 tcagcaagaa ggaattaaat taaatggttc agccattcaa tgccgaatca ctacagaaga
    10441 cccgttgaat aactttttac cagacacagg gaaaatcgat acgtatcgtt cacctggtgg
    10501 ttttggcgta cgtctggatg tcggaaatgc ttacgcgggc tatgtagtga caccgtattt
    10561 tgattcattg ctagtaaaag tatgtacgca tggtgctacc tttgaaacag ccattcagaa
    10621 aatggaacgt tgcttgaaag aatttagaat ccgtggagtg aaaacaaaca ttccatttat
    10681 gctcaatgtc atcacacatc ctgaattcca atcaggaaat gcaaaaacaa ccttcattga
    10741 tagtacagca acattatttg aatttcctcg actacgagac cgcggaaaca aaacgatgaa
    10801 atatatcggt gaaattactg tgaatggttt tccaggtatc gaaagtggcg aaaaaccatt
    10861 ttatgaagaa ccacgtatgc ctaaagatct aatcacaaga actgattacg taacggctaa
    10921 gaatgtatta gatgcgaagg gtgccgatgc tttggtcgaa tggatcaaag gacaggaaaa
    10981 tttgttgttg acggatacga catttagaga cgcccatcaa agtctgctag caactcgtgt
    11041 tcggacaaag gatttcaagc agattgcacg tttgactgga gaaggtcttc ctgaactctt
    11101 ttcaagtgag atgtgggggg gagcaacatt tgatgtggct taccgctttt tgaatgaaga
    11161 tccttggcaa cggttgagaa aaattcgttc tttaatgcct aatacgttgc tgcaaatgct
    11221 tttcagaggt tcaaatgccg ttgggtattc taattatcct gacaatgtat tggtggaatt
    11281 tgtcaaagaa gcagcagcgc aaggaatcga tgtatttcga atctttgata gtttgaactg
    11341 gactccacaa atggaaaaaa gtatccaagc tgtacgggat actggtaaaa tcgcagaggc
    11401 agctatttgc tatactggcg atatcaacga tccaagtcgg gcaaaataca atgttcagta
    11461 ttataaagac atggcaaagg aactagaaca actgggtgcg catatcattg caatcaaaga
    11521 tatggctggg ctgcttaaac cgcaggcagc ttatcgtttg attagtgaat tgaaagaaac
    11581 gacagatctg ccaatccatc tgcatactca tgataccagc gggaatggaa tcatcactta
    11641 ttctgctgct tcaaaagcag gagtagacat cgttgacgtg gcaatgagtg caatgagtgg
    11701 gaatactagt cagcctagca tgagcagctt gtattatgca ctggtcaatg gtccacggct
    11761 gccagaaatc acgattgaaa atgcacagaa attaaaccat tattgggaag atgtgcgcat
    11821 gtattacaaa ccgtttgaaa acggcttgaa tgctcctgaa acagaagtat atatgcatga
    11881 aatgccagga ggacagtatt cgaatctaca gcaacaggca aaagctgtcg gattaggtca
    11941 tcgctgggat gaaatcaaac aaatgtatca tactgtcaac ttgatgtttg gcgatatcgt
    12001 taaagtaact ccatcatcta aagtagttgg agatatggca ctatttatgg tccaaaatga
    12061 tcttacagaa gaagatattt atgaaaaagg cgaaacacta agtttccctg aatctgttgt
    12121 gactttcttc caaggagaac tagggcaacc cgttggcgga tttcctaaaa aattgcaaaa
    12181 gattattctt aaaggcagac ctgcattgaa tgaacgtcca ggtcttttgg ctgaatcagt
    12241 cgacttcaac gaagtaaaaa aagaattggc agaaaaaatt ggctatgaac caaaacaaga
    12301 cgaagtatta agctatttga tgtatccgca agtctttttg gactatcaaa aagcatataa
    12361 tcaatttggc gatgttacat tactggacac accaacattt ttccaaggta tacgtttggg
    12421 tgaaacaatc aatgtacaaa tcgaacgcgg aaaaacgctt atcattcgtt tagatgaaat
    12481 cggagaaccc gatatcgaag ggaatcgcgt actgttcttc aatttgaatg gacaaagacg
    12541 tgaaatcact atcaaagata attcgatcat cagtacggtc caaacccgat tgaaagctga
    12601 accgacaaac cgagaacaaa ttggagcaac gatgtctggt tctgttttgg atgtattagt
    12661 gaaaaaaggc gataacgtta aaaaaggtga tacgttgatg attactgaag cgatgaaaat
    12721 ggaaacggcc atcgaagcac gttttgacgg agaagtcgca catgtatatg tgtcttcagg
    12781 agacactatt tcgtctggtg accttttgat tgaagtgact gagaaataat aagcgaaagg
    12841 tggggaaaca tgaaaagact gggtttgttt tggctgattc tgctattagt tattgtggca
    12901 ttctacatgg aaccagtctt gtttccgcca aaacttgaag tgaaggaaaa ctctgatcaa
    12961 caaatatcaa accaaatgac aacgtggaaa taccaggagc ttaaagcaac tggtttttct
    13021 acttatatcg gaaaaccgat cgatcagctg gaaaaggatt ttggccaagc ctatgatcga
    13081 ttaactagcg gatttggttt tgaaacaaga tattatgaaa ataaagctag tcatctcaca
    13141 tttgaagcga acatccaaga cagcagagtc acaactgtta aagtactgca aacagggcct
    13201 gctgatatcg cgccgtttta tttagggatg acgatgcaag atcttacgaa aatcactact
    13261 atctatacta atttcacttt tgagtaccat gatacagaag ttggaatcga actaatggaa
    13321 gaggatatga attatcgtcc gctaattgct tttgataacg acacttttgc tattttattt
    13381 tttgatcaaa caacaaacgg cctattttca gttgtgtatt tagataaaga tagtcttttg
    13441 aaactattgc catatcaagt gtttggtgaa ggccttccac attatcaagc agataaatcg
    13501 gctgattggg aatcaatcaa tcgcgttaaa gaacgaaaaa gtacgacttt attaaatatg
    13561 cttagaagag aagacgaact tcccatatac aatcaaacac ttgcctttca aaaccacagt
    13621 cagctgctac tacatgattt tttgacaagg cctgaggaaa tattgtcaga ggaacgtatg
    13681 gctgattggc aaaaatcgct aggcagtgct caaacgacag tgaaattctc actgacgaat
    13741 gatgaattgg agaagttaat cagtaaacaa aaattagaaa atactagtgg gctatttatt
    13801 catccggcga ttgacgctac tttttcgttt ttatattggt acagcgatcc ttacaaccat
    13861 gaccgtttca tggacccggg aacggattct gtaggcattg ctttttctaa agaaaatatg
    13921 ttagtcttat tgcaagaaga aaacggagaa agtagtgata gcagtgatcg ttaacgaaga
    13981 gctgtttcaa ctggaggatc aatgcctcca agtcgctgaa atgataaaaa agagtaaaac
    14041 gatggcttct tatcttgaac ataaacagtc aatggaagaa tctgcaaaag tttgccagtt
    14101 gaaagaagcg ttcttgtcta agaaagaagc atttgaaaat gtagcggcat atggtgagta
    14161 tgcacctgat tatcgtgaaa aaaaacgagc tgttcgaaaa gcaaaaagac agcttgatat
    14221 ggatgagcat gttgcagctt tccgatatag tgaaacacag ctgcaaagtc tgttggatca
    14281 aattgtccaa gtacttgccg aagaagtttc taatgaaatc aaagtagatg caggtagccc
    14341 gttttttgaa acgaaaaaac actttggatg cggaggtaat tgtcatgcaa ttagacaata
    14401 gtgaatttga aataacaaaa cgacgcgggc tgatcgtatg ggtctacagc ttaaaacaat
    14461 taaagaatct gaaaaagttc ggcttgttgc attatgtctc aagaagaatg aaatatgtcg
    14521 tgctttactt aaatgaagag acattggaag aaacagaaga acgaatccgt aaattacatt
    14581 ttgttcgaaa cgtagaacgt tcttaccgac cggatatcga aatgaatttt gccgaaaaaa
    14641 taggaaacaa aacagtatta aaagacgaag gtttcgaaat tgaagagcta aatacagaaa
    14701 tccgattagc tgaacatctt taaaaatctc ttatgagcag tttttaaata aaaaataact
    14761 tggataacga tcgatttttg atcattgtcc aagtattttt ttggtatact agtaccagta
    14821 ccatttctaa ggagtgagta gatgcggata atatcaggaa attatggcgg gcgccgttta
    14881 aaaagtttag caggggcgaa caccagacca actacagata aagtaaaaga atccatattc
    14941 aatatgattg gaccatattt tgatggagag acagtattgg atctgtttgc aggaagcggt
    15001 ggtttagcta tcgaagcagt atctagaggc tgcagtcatg cggtatgcgt agacaaaaat
    15061 taccaagcgc taaaaatcat caaagaaaac atcgaaatta cgaaagaacc agagaaattt
    15121 accacaataa aacttgatgc ggacaaagca atcgaacggc tagcagcgga gaaacaacaa
    15181 ttcgactact tgtttcttga tccgccatat gcaaaacaaa aaataatcga tcaaatccat
    15241 aaaatggaag aactgggact tttttctaaa accgcagtta tcgtatgcga aacggataaa
    15301 acagtcgaat tgccagaaac tgtggctgga ttccatcaaa taagaaaaca gatttatggc
    15361 ataacggccg taactatcta tagaaaagag ggaaatcaat gaaacgtgca ttattcccag
    15421 gaagttttga tccttttaca aaaggtcact tagatacggt ggaacgtgcg gctaaattat
    15481 tcgatgaggt agtaattggt gtttttatca atacttcaaa gaaaagtttg tttcccccag
    15541 aagaaagaat gacattgatc acaaaagctg tttctcatct tcctaatgta aaagtcatgc
    15601 atcaagaaaa tcaattgacg gtagaaacag caaaagagat tggtgcagat gcgttgatca
    15661 gaggcatacg aagtatcaaa gactttgaat atgaacgaga gattgctcag atgaatcatc
    15721 atctatatga tgaattggaa actgtcttct tattagcaaa accagaatat agtcacgtga
    15781 gttccagtat tttaaaagaa gtactgcact ttggcggaga tgtgagcagc tacttaccgc
    15841 cagtgatcaa cgaagcttta gctggaaagc gtgaatcaaa tgaaagctaa acaatggttg
    15901 aaaacagtac tgattatcat atttggaatg ttgatcattg gatttgtagt agttcccttg
    15961 ccgtattatg tagaatcacc gggagccacg atcgatctga aagaactaat tacggtcaat
    16021 gataaggaag ataaaggttc tgggtctttc tctcttacct ctgttggtat caggcaagct
    16081 acaggtttct tagctttaaa agctaaaatg accccttttg aagaattgat cagtgaagaa
    16141 gaattgactg gtggggcgac aaatgaagaa tataatcaaa tgcaacagta ttatatggat
    16201 tcttcccaaa atgcagcaat cgaacaagcg ttaaaacttg cagacaagcc ttataaaatg
    16261 gaattcaaag gcgtatatgt catgagtgta gagaaaacat cgaatttctt tggcaagctt
    16321 tcagttggag atacagtaac aaaagtagac gggaaaagct ttcaaagtac agaagcattt
    16381 atggattatg taaaaagcca aaaagttgga caaacaatcg aaatcagtta tttgcataat
    16441 ggtgaagaaa aaacagcttc tggtgatctg atcgagttac caacagataa aaaggcagga
    16501 ataggtatca cgcttactga ccataccgag atcgaatcag atacagatgt tcgtattaat
    16561 tctggctcta tcggtgggcc atcagcggga ttgatgttca ctttagaaat ctatgaacaa
    16621 gtcactggta aaaacttgcg acatggtaag caaattgcag gaactggaac gatcaacagt
    16681 gaaggagaag ttggccgaat cggaggaatc gataaaaaag tggcaagtgc ggataaagct
    16741 ggcgtagaga ttttctttgc accagatgat acgatttcag acgatgttaa gaaagaatat
    16801 cctgaaataa aaaccaacta cgaagaagca aaagctgccg ctaaaaaatt gaattcctca
    16861 atgaaaatcg taccagtaaa aacagtacaa gaagcgcttg actatctaga gaaaatgaaa
    16921 tgaaactaaa taattaaaga ggggttgtga cacgagttct gtcacaaccc ctctttttcg
    16981 tcaaaaatct tcacgataaa aataaaaaca aaagaaaaaa ttgacgtaat tagatgttga
    17041 agggacgatt ttgccagagc tcactgtttc ttcaatttta aatgaaagga gtaatgaatg
    17101 atttttactg gtcattcttt gaaaaaagaa caatgataga aaaaatcagc aagaaaatac
    17161 tagcttcacc caaattgatc atttggatgt ctatgatagt tcttatcttt tgtatcgcaa
    17221 gtggtttttt attatcaaat ttgctatttt atagagaaac agaagagact gctgcaaaag
    17281 aaatactttc ttcgtttagt tccagtggag aagatccatc agctgaagga aacgatgtag
    17341 aagaggatgc tcgtccaaag gtcatgtaca ccgatatcaa aggaagtgtg aaggagcctg
    17401 gtatctattc cttttcttca gaagaacggg tctatgatgt actaaagcgt gcaggtggat
    17461 tattagaaga agcggatagc gatcgaatca acttttctgc gaagatagaa gatcagcaag
    17521 tcctatatat ccctgctgta ggtgaagagc tgccagaaca tcttgatcag ttggcatcct
    17581 cggaaggaaa acagcctaca gcagattccg agcctcccaa aataaatatc aatacagcca
    17641 ctccatcaga acttcagcag attcctggga tcggatcagt caaagcgcaa gaaatcattc
    17701 gatttagaga agaaaacgga tccttccaga aagtggaaga cctgcaggag atttctggta
    17761 tcggtgaaaa gacagttgaa aaactaaaaa acttcgttac aattaaatag aatgattcaa
    17821 aatcgactgg cacgagcaat aaatggacgc taatggatac taagaataat ccaattctaa
    17881 gagcttggca ggacgaggag gtagtatttt tggataggaa atagcttgat tgttcgcaaa
    17941 gaaacattaa aaaaaccaat gctgaatgtc tatctccaaa ataaaatatc tgggattcat
    18001 attatgaata cggctgtcag cggcaataac agtcaagctc tacgagaacg ctttgccaaa
    18061 gatgtactta gctatacagc agataaagta tttattctca ttgggacgaa tgatctagca
    18121 gagcataaac aactttctaa agaaacgtac caaaaaatct gcagtggatg atagttcagc
    18181 tgaaaaaaga ctatagttct gatcatgctt tctttatcac gccaccagca gtcgatgaga
    18241 caaaacaaaa atatcggaat aacaagttga tcaaagaata tatctctgtt ctcacagaag
    18301 tagcaaaacg ccatgattgt gaagtatcga tttttatacg gaatttcttg aacaaatgga
    18361 ttcgaagaat ttttataatg ggctaatgga tgacggactt catttcggca gtgaagggta
    18421 ggatcttttg tcagacagga tgattcgtta tatttaaaga atattttgtt taagcaatga
    18481 gtacttttta atcaacttga aaaaacggag gaatataaaa catgttagaa aaatcttcaa
    18541 aagcatgcga tacgaataaa gaaaaagtta ctgataagtc acataatgaa cgcattcctt
    18601 gggaccagta ctttatggct caggcagtac tgttatcttt acgtagtacg tgtacacgtt
    18661 tagaagtagg agcgacactc gttcgagaaa aaaggatcat tgctggcggc tataatggag
    18721 cagtcagtgg agatgtccac tgcatagatg aagggtgtta cgtcgtggat ggacactgct
    18781 tgcgaacgat ccatgcagag atgaatgcac tcctacagtg tgcaaaactt ggtatcccga
    18841 cggaaggttc agaaatctat gtcacacatt ttccatgttt ggcctgtaca aaggctttgt
    18901 tgcaagctgg tgtgaaaggg atcaattatc tccatgacta tcgaaatgat ccttatgccc
    18961 aagcgttgat cgaacaatta ggtgtatctg tacaccaagt tgctttagac tcacagcatt
    19021 tttcaaaact tcaaaatgaa ttaacaggaa aacatgtcta gcgttttaga aaggcatgtt
    19081 cgaaatcggt tgatttttcc ggcaattacg tcgatgatta tctgtttgtt gtattacact
    19141 gacaattggg gttatcgttt gcttcttgcc ggaatttttt tatggttttc gctaaaagaa
    19201 ggacgtttta tcgcgtactt gtgtattttg tgtagcataa cggtatccat ttcttatagg
    19261 ctacaagagt attcattttt agaggaaaaa acaacatctg cgcaaattac tgtatttaca
    19321 gatacgattc gtgcaaatgg taattttttg acaatggaag gaaggttatc agagaataaa
    19381 caaaagctcc agctttctta tcaagtaaac acagaagcag aattgaaaac atggcttaac
    19441 ttccgaggaa agggaataga attgactgct gaaggcgagt tcatctctcc agcaaaaaag
    19501 cggaatcttt ataattttga ccaagaagct tacttcagga accatagaat ttctggacgg
    19561 ttccaaattc aaaagattga acagatcacc atgcaaaatc actggcgaaa ctggcttcag
    19621 agaaaaagag gatcttttat ctcctttgtc caatctgaat ttccgcaaaa aacaagtgta
    19681 tatattaatt ctttattatg gggctataaa gatgcaggat tcagagaacc agaagaaatt
    19741 tttagggaga gtggtttgct gcatctgttc agtctatcgg gtctacacat ccaattttat
    19801 ttagggtggc tgtattattt gtttcgacgt atcggatggc cactacacgt cagtattatt
    19861 ccgttgagtc tactaactat ctgttatgtt tctattgcag gaagttctat ttctgttttg
    19921 cgtgctagtt tgctgttttt attgcgattg ctagtcaaat tgctgaataa gcattattcc
    19981 accatggact tatttgcagt tgtcttttgg cttcttttac tatttgatcc tcgatttctt
    20041 tttcatgcgg gtggacagct tagttttttc atgagttttt tgtttatcct gatttctttt
    20101 gatggagatt ggttgaaaaa aacggcctct cacgtcctgt ttactatgat cacgatgccg
    20161 ttgattgtat ggcattttta tgagtggcct attttaggta gtctttttac attactgatt
    20221 gcaccggtat tcaagtttct ttttcttcct ggtgtgttat ttctcacact cttcaatcgc
    20281 tggctgccgg aaactttact tttgctgata gaagagggat taatcttatt tgagcgaata
    20341 atcgattttt cttcagggtc gtctctcatt ataggacgtt tgccccttgt gctatctggg
    20401 atgtatattt tcggattact gatttgtatg gataggttga aagaacaacc tgtgaaacac
    20461 ttgttttatg ttggtctttt ttcttttatt tttttgactt tgccattttt acgcttttct
    20521 tcaacgatcg cttttatcga tgtcggtcaa ggagacagta tctttttgca aagtcctttt
    20581 cgaaaagaaa cgattttgat cgatactgga ggtcagttac actttgaaag agaaaagtgg
    20641 gcgcaaggta tgatacgtcc tcctgctgaa agaaatatcg ttccatatct aaaaggtcag
    20701 gggataagtg taatcgataa actcatcttg acacacgacg atacggatca tgtgggagaa
    20761 ttacccacgt tatctcagca ttttactatc aaaaaaattt atatcggttg gggcgctgga
    20821 cgaaatgagc cattacaaag gcatttatct gaagctcaaa aaaatggtac tgagatcatt
    20881 gaaatcaaac atggggacag gatcagcggt tattatgatc tttatgtatt gactcctttt
    20941 gaaaagggag agggaagaaa tgaagattcg attggtctat ggatggaata taacaatagg
    21001 cgctttttat ttttaggaga tttgaatcaa gcaatggaaa aacaattgct tttgatttat
    21061 cctaatctga aagccgatgt agtcaaatta ggtcatcacg gtagccgtac gtcaagtaac
    21121 acagattttc tttcccatat tgaagcaaaa catgggatta tatcctgcgg cgtaaataat
    21181 cgttatggtc acccgcacag cgaagtattg gagacactag aagaaaatca gataaaaaca
    21241 tggcgaacgg atcaacaagg aatgatcacc tatcgctggc atccagtttt tcatcctcat
    21301 gggctagtgg aaacaatgat agactagtaa ttctcatctg ctcatggtag gataattttt
    21361 gaaagtggtg agtaatttga gtatacaagc agagttacaa aaaatacgta aagaaaaact
    21421 tgcatcttgt tacttgattt taggaacaga aaagtttctc caagatcaag ttcggacaga
    21481 aattgtcaaa aaaatccaat tagagggcga agatgactta aattttttaa gttttgacat
    21541 ggaaaatgca agtttagatg aggtagtagc tgaagcagag acgttgccgt ttttcggaga
    21601 tcaacggtta gtattcgtag agaatcctta ttttttaaca ggtgaaaaag taaacaatgg
    21661 aatcgaacaa aatactgatt tattgacggc ttatttgaaa gaaccattag aatcgactgt
    21721 attagtattc tttgcgccat atgaaaagtt ggatgaacgg aaaaaagtaa cgaaacaatt
    21781 gaaaaaaaca gcaattatca tcaatgtcca acagttgaat gaaaaagaag tacgtcaata
    21841 tctgatgaat accttagaga attcctcaat caaaatggaa cgaagtgcaa ttgatttatt
    21901 cttgaggttg accgacttag atttatccaa aatgatgaga gagttacaga aattggtcct
    21961 ttatggacaa aatcaacagc aaatcacggt aagagaagta gaagaattag ttcctaaaac
    22021 gttagaacat aatatttttg acatgaccca atatatttta aaagggaaga cggaacaagc
    22081 tttgcgttta tacgaagatt tagttatgca aggggaagaa acaatcaaaa ttaatgcgat
    22141 tttgctttct caattacgtt tatttttaca gacaaaattt cttgtaaaga ttggttatca
    22201 gcaggcaaat attgctgaaa cattgaaaat ccatccatat cgtgtaaaac ttgccatgca
    22261 ggaagtaaaa aaattcgatg aacatttgct ggtacagttg tttgatcaat tagttgagat
    22321 ggattatcaa atcaaaacgg gacaaatcga aaaagaatta agttttcaac tattcgtttt
    22381 gcgcacaggg cagttgttaa gaaatcgata agaagaaaga cgagggcaaa gcatgcggga
    22441 aggtcgtttg tttttgtcct ttttttgaca aataaaaaaa tcggccgaag ccgattcttt
    22501 tatttagcga tttttttgct taaacgagat ttgtcgcggt tagctttgtt tttgtggatt
    22561 agaccttttg attcagccat atcaatagct ttaacagctt ctttatataa cgcatctacg
    22621 ttgtcagcac ctgaagctac agcatcttca aattttttga ttgcagtacg catagcgctt
    22681 gtttgagatg aatttttcac attagcgttt tcgcttgtac gaacacgttt gattgcagat
    22741 tcaatgtttg gcattagttt cacctccgat aacttaagtt cttacgcgtg ttaccgcata
    22801 attcaacatt cattattata cctaaaggca gcgtgctttg caatatgaac atgacaagtt
    22861 tttttcctta acagtctttt attaggattt tttttcagta aaaacggata gtacaagctg
    22921 aaaaaaatca aaaagacgct attgtgggag gcaatagcgt caaaccaaac tagattttta
    22981 tttatctatt taaacaaagt ttgaatggga atgtttggta ctaagttatt atataacaaa
    23041 gagaaccgtt tgcaaagttt tttttgaaaa gggatacgat tccctgttgt tctttgtttt
    23101 tctattaaga aactgcagtt attttataac cagtatcctt tgatcagtaa aggctattct
    23161 ggtgtaccga ttattttaat gtattcatcg atatttttta tcaacatttt ttttatgacc
    23221 aactggttcc gacggttcca tttgccactt acttccagca catatttatt taggggcaaa
    23281 cgtaagatat attttgccaa atagcgatct ttaagcaggc cattgatcac acggtcctca
    23341 agctgcaagt tgatcccagt cagcatcatt ggctgttctt gtaaaacgta cgtatcaatt
    23401 aaaagacctt caaaagtttc cacaagcgtc ttcctttctt ttcaaagtaa cctgaaaaca
    23461 ggcactaatt cttccatttt gatattcgtt gaagtggatg cgatcattct ttcttttgtc
    23521 cactgtaatc tagtataaca aaaaagagca gaagagccaa aaagaaaaaa gagcatcgaa
    23581 ttctatagga agtctcattg gcacggccta aattattaca aaacattgat taaaaaaagc
    23641 ccctgcatcg caggagcatc ccctatatcg ttttaatagg tcaatcaaaa agcagtatca
    23701 aacacatttt gtatcaaatc aggaaataat agataagcta agcccagaat caaaagtacc
    23761 gctagaagaa accagccgat tgttcctatg aaatacttta atttttcgaa cattgtatca
    23821 cctcgttgtc agtatatcac ggatcagtaa attgggggtg ttaagaatct cctaaaatag
    23881 acaaaatttg ttctattttc ctaaagcaaa tacttgtaag atacagtaag tgataccaga
    23941 agcaatgacc ggtccggcgg cgattcctct taagaagaca acacctaaaa tcgtgccgaa
    24001 aacaagtgca acagtgattt gtggatcaga agcaatcaat cctacaccct tggaagataa
    24061 gacagccact agcccaccgc aaaaaacagc tacccagcca ataggggatt taaaggcttc
    24121 taatagatca cgtagaccga tttctcctgt tgcgataggg attagaatag ccgctgtgat
    24181 gatcgtcacc ccaatattga ttcctttgcc ttgaagccaa gggaaaaagg aacgggaggc
    24241 tggtatgagc ttcataatca aaacaaaagc tgccgctatc atcagacttt gatttttagc
    24301 taaaattgct acaagtaaaa taattcctaa aaaaagccaa ctttccatga aatatattcc
    24361 tcactttctt cgaaacaatc ataacatgtt tttagaaagt tacaaaagta ggaattcatc
    24421 cttcaagacc tattcataag ctgttttttt cataaaataa acaaaatcat aaaaaaacac
    24481 tttgcttatt tgacagaaga cagccgcgct ttgtatagtg gacaagactt acattttttt
    24541 gaagaatcag aaagatcagg taaaggtctt agtaaacgca agccattcag aataaccaat
    24601 attgtgcttc cttcgtgtcc tacaactcca agaggtagat ttaaaaattg caggaagtta
    24661 gaagcaatca acaagagaat aacaagaatc gaaaaaataa tattttgttt gatgatccgc
    24721 cgcattttta aagatagccg atgactgtag acaaatttat ttaagtcgct tttcattaag
    24781 acgatgtctg cgatatccat agcggcatct gtcccttgcc ccatagcgac acctaaagaa
    24841 gcggctgcta aagcaggcgc atcattgact ccatcaccaa ccatcgcatt tacttggtaa
    24901 agttcattct cttttttgac tagttgtgtt ttttcttccg gagtacagct tgcatagtaa
    24961 tgttcaatcc ctacttgttc agcaatggcc tttgctgtgc cagaatggtc gccggttatc
    25021 atacttgtat ggatgttttg ggatttaaag taggaaatta cttgttgggt atttgctttt
    25081 ggaatatcta gcagacctaa aacagcaatt aactgctgat ctttagaaag ataaatcacg
    25141 gttttgcctt gattttccaa acgttcgatc atttccgcaa tctctttgct gattatcatc
    25201 gtctcaggat catatgcgtg cttgccaact ttccattgcg cacctaaata ggtagtttgt
    25261 aatccaaaac cagtaatttc ttctattggt aagtattgga tttcttcagg tattgtgcaa
    25321 tcgaaatatt gcaaaatcgc atgagcaagt ggatgcgtcg ttttttgttc catagcaaca
    25381 agaagttgtt gtgccaaatc cttttcatca aagaaaaagc tgtcagttac aactggtttt
    25441 cctttagtga gcgttcctgt tttatcaaag gcaatcgctt ttaattcaga aagcttttct
    25501 aaatggatac cgcctttgat taaaatccca ttttttgcag aatttgaaag agcagctaga
    25561 gaagctggtg tagcagatgc gacaagcgca caaggagaag ccacaactaa taataccatt
    25621 cctcgataaa agctttcatc ccaactccaa cctaggaaaa agtgggggag aaggatcatt
    25681 agaggaacag caagcaaaat caattttaca taattatttt cgattcgttc gataaatgaa
    25741 gctgtttgag ttggcgtatt ctgcgcttct tctactaatt ggataatctt agcaaagaca
    25801 gtttcggaac tcgtttttgt tactcgaatc gtgatgggat ttccttgatt cagcgttcct
    25861 gcatacatct catcgcctaa atatttttct acaggcacag attcaccgct taatgcagac
    25921 tcatcgatcg ttgcctgtgt gctttctaaa aagccatcta tcggaacagc tgctccttta
    25981 gcgacaaata ccaagttatc cacctttagt tgagaaacat ctacttcttg catttgtcca
    26041 ttttctttta ataattgggc tttttgtggt tgaagattca tcaaagcagt aatttctttt
    26101 tgacttttat tagttgtgta ttcctctaat gcaccgctta aacaaaagat gaatgtcaac
    26161 attgctcctt caaagtaatt accgatcaaa caggcaccaa tagcagctaa agccatcaat
    26221 aaatccacat tcagtgtccg atcattccac aattctttca agccttccga tgtttgttta
    26281 tatccaccga tgataatggc actgatataa aaaagtgggg tgatggtaag agagaatcgg
    26341 tcgaagataa aaccgaagat cataaataaa aggcagccga ttgttgctgc aaaagctttt
    26401 ttctgattag tcatcgttat tagacctcct tgtcaaaaca aggatagcat aataaaaatg
    26461 taattgataa taataacagc taaatgagaa cgataatggt tatcatttgg tgattaagct
    26521 tgatgttgtt tttattattt ataatgatta ttattaaaga aaataaaaaa ccagaacatt
    26581 tctcaaataa gagaagtgtt ctggtttttt ctacattgca ataaaccaat aaccaatcaa
    26641 aatgatacca aggataatac gataccagcc aaaagcggta aagtcgtttt tcttcaaata
    26701 gcttagtaag aatttgatag ctaagacgga aaccacaaaa gatacgaatg ttcctacaag
    26761 aagaacaaca gtttcttcca ttttgaatga attcccttca gctaaatatt tgacgatctt
    26821 caagccgctt gcgccaaaca tgacaggaat ccctaagaaa aatgaaaact ctgtagcgac
    26881 aaaacgcgaa gcaccaatca agatagatcc taggatcgtt gcgccagaac gggatgttcc
    26941 gggaacaagt gccaagactt ggaataaccc aacaagaaga gctgcttggt acgtaaagtc
    27001 attcagagaa gtccaacgag gtgttttgtt tttgttgcgt ttttcgacaa taataaaggc
    27061 aataccgtaa acgatcaaaa caatggcaac tggtaagaac ttgtggaaat ttgcttctaa
    27121 ccaatcatcc aaaggcaaac caatgatgat ggctggtatg caagcaacga tcactttaaa
    27181 ccaaagtgtc cacgtatctt ttttttctac cgttgattta cttggagaaa atgggtttaa
    27241 cttgtgaaaa taaagaacga cgacagccaa gatcgcccct aattggataa cgacattgaa
    27301 catcgacata aattgtgaac tggcatccag tttgatgaat tcatctgcta ggatcaaatg
    27361 accagtacta ctgatgggca gccattccgt tattccttca ataataccga gaatcactgc
    27421 tttgataata tttgcaaaaa acataaattc cttccttccc aattaagata catcttatag
    27481 tataccgtta ttttttgaga aacccaatct gttttttaat attaagaaaa acttgagaaa
    27541 taaagcagat aagtacacga agagaaaaaa gaagacaaag ccctcgttca gactttgcct
    27601 tttatgattc tctggtaaaa attaaaatat ccccattata gaaacgagtc gcgactatgt
    27661 gttttcccgt tatcgatata agacgtatca ttgatgccgt ggatagaaaa tcgaccgttt
    27721 gcataagtga cctttgttac gctgccattt aaaagcccag cacgaacagg ctgtttagga
    27781 tcgatcagat taagcaaaaa ggcgatcgtc aaaccatgag atacgactaa aacatttcca
    27841 ccaccatttt tttcgcagtg atgggcgata tcttcaaatc ctgtccatac acggtctcgc
    27901 aatgtttcat aagtttccgc ccacccagcg gtatctaagt tgtatacagc attagcaatt
    27961 tgttcaaaag aaacttctgt ggtaaacatt tcatcatatg ttttaaaatt caatacacga
    28021 gggatcacac cccacatttc catgtcatat ccaccttcaa gagacccgaa acaccattct
    28081 ctaatccttt tttcaataaa ataaggaatt tcttttccct tagaatgttc tcctagaata
    28141 atacgggcag tttcgattgc acgaccactg tcactagaat aagcaaatga aaagtcgaca
    28201 ttttttaacc cgcgacctaa ataatgaatg ccttcttgtc cttgctttgt gaggggtgta
    28261 tcacaccagc cttgtactcg ttcaattgta ttgaacatcg tttttccgtg tcgtacgata
    28321 taaagtgtag tttctgtcat tttattctcc tcctaagtcg ttttaacgat tgaaaaaagg
    28381 atttgtcctt ttttcctgtt caatcgtcgt tgcatcgcca tgtcctggat agactgcaaa
    28441 ttcatcaggt aatacaaaca gctgggtccg aatactatga agtaattgtt ctaaattccc
    28501 tgtatacaga tcggttctac cgatacttcc tctaaacagg gcgtcgccag aaacgacaaa
    28561 atcatcaaaa ataaagctga cgcttcctat agaatgccct ggggtaggga cgacgcgaaa
    28621 agacatgttt cctaaagtat aagttttcat ttcaaattca tattctgcag gcgagacaat
    28681 aacatcagca atatcatcat gtctaccgag tccagaaaga ttcaaaatcg gatcacctag
    28741 ccatttttgc tctagtgggc tgacataaac tgggatttga taatacgcgc gtaaaggttc
    28801 cactgcaccg atatgatcgt agtgagtatg ggtaagcaaa atcgctaccg gctgttggtt
    28861 tgtttttttg atttcttgtt tgattcgttc cgcatcttct ccagggtcaa tgatcaaaag
    28921 tgcttcttcg ttgtagacca aataacagtt ttcttcaatc attcctgttt ttaatctttc
    28981 gatatgcatg tgcattcctc cttgatagcc aaaacttctt tcatccatta gtataacgca
    29041 ttcattgcta ggaaacaaaa acaggtactt atttctcttt acttatctga attatcagac
    29101 tatatgaaaa gtatttggat attctaaaga gaaggaaagt aagttttcat agcattttaa
    29161 tatgtccttg ttacattaga ttcatccaaa aatccaaatt ctatatccta tatccctgcc
    29221 taatcagcag ggtgtttttt tatgtttgga aagagagctc tttttgctaa actaggtttt
    29281 ttcaggtaat cttagagaga aagaggtggt ggaaatgatc aaacagtcaa aaatatctgt
    29341 gcggcactta gtagaattta ttttgcgacg tggaagtatc gacaatcgta aaaaaagtaa
    29401 ccatactgca ttagaaggag cgagaatcca ccgcaagctc caaaaagatg cgggagaaaa
    29461 ctatgaaaaa gaagtctttt taaaaacaac agttcaatta gatgaatatt acctgatcgt
    29521 tgaaggccgt gcagacggta ttttcaaaag agacggtatt tattatatag atgaaatcaa
    29581 aacctccgaa ccacgatttg aagatttaga accagaacaa gtggaacttt tctttcatca
    29641 agcaagggtg tatgcctata tctatagtca agaacatgat ttacaagaaa taaatctgca
    29701 attgacttac ttccagacgg cagaagaagt gatcacgaga aaggttgaac atcagacaag
    29761 agagcagtta gaaacttttt ttaaaaagct gactgaagat tataaagaat ggcttgtttt
    29821 tcaagaaaac tggcgcactg tacgcaatgc ctctttgatg gctttgaaat tcccccatga
    29881 tgagtatagg aaaggtcagc gtgaactagc ggtagctgct tataaaacga tccgtacgaa
    29941 acagaaattg tttgtagaag cacctaccgg aacaggaaaa acaatctcta cgttattccc
    30001 cgctttgaaa gctgtagggg aagaagaggg agaaaagatc ttttatctaa cggctaaaac
    30061 aataactaga caagtagctg aagatgctat gacagcatta aaagatacag gagctgaagt
    30121 aaaaagtgtc actttgacgg cgaaagacaa gatctgcttt ttagatgaga cgacctgtaa
    30181 tccagatcag tgcccatatg ccaatggtta ttacaatcgg attaatgaag gtttatggga
    30241 tcttttgaat catgagaatc agatcactcg tgaagtcatc gaaacttatg caagaaagca
    30301 tacactttgt cctttcgaac tatcattaga tgtcagtatc tggtgtgacg taatcattgg
    30361 ggattacaat tatttatttg atccaactgt ttatttaaga cgtttttttg aagatgaaaa
    30421 aaacgaggac tatctgtttt tgattgacga agcacataat ttggtgaatc gttcccgaga
    30481 gatgtattcg gcagaactat cttatgaaaa aacgaaacga agtaaggaag ctataccaaa
    30541 ggaattcaaa aaacttcatc gccgtttcaa caaactatta aaagaatttg attcgatccg
    30601 agaaatagca aaagaagatc attgggatta tcatcatcaa aaagcaccgg ctgaatcttt
    30661 agtaaaggca ggttatcaat taagcgaaaa aattaaagaa tggctggcag aatttcctga
    30721 gcacccatca caagaacaat tgcttcctta ttattttgat ttgcttcatt ttttgaaggt
    30781 cagtgaattt tatgatgatc actacgaaac aacagtcgaa aaaacgtatc gcgatctgat
    30841 agtaaaagag ttttgtattg atccaagctt gtttcttgag cagtcgttgg ataaaggaaa
    30901 aagcagtatt cttttttctg ctagtttttc accactctct tattatcagg aaacactggg
    30961 gggacaaaaa agtctggctt acaaactgcc gagtcctttc ccagaaggaa accagcaagt
    31021 tttcatcgca aattacctag agacgactta tcgaaaaagg gaacacagtc tggcaaaatt
    31081 agtagagacg attcatgctt ttgctgatgg aaaggtcggg aattatttcg ttttctttcc
    31141 ttcttatcaa tatttagatc ttgcggtgga agcattcaga cagcaatatc ccgacagcaa
    31201 tgtgctaatc caagaaacgg acatgaatga ggaagaacgc gaacgatttt taacaaaatt
    31261 tcaaacaaat cccgctgaaa cattgattgg attttgcgta cttggcggga ttttttcaga
    31321 aggaatcgat ttgagagggg accgattgat cggcagtatg atcgtaggtg taggtttgcc
    31381 gcagatgaat catgagcagg aactgatcaa atcatattat gatgagaagg aacaccttgg
    31441 atttgcttac gcttatcagc ttcctggaat gaacaaagtc ttgcaagctg gtggtcgagt
    31501 gattcgtggc atggcggatc aaggaatcgt tgtattggca gatcaacgat tttctagttt
    31561 ttcttatcga cgtttattcc cacaacattg gcaaacagca agagaagtac gaagtgttgg
    31621 ggaattggaa gaaggaatac agcgtttttg gttatcaaaa gaagcagtaa atcaaaaaaa
    31681 cgaggaatga cagacgtcag cctcgttttt tgattaacga tagttagtga attgcaactc
    31741 gattggtaag tcaagtttac gtaataaagc aatcacagct tgtagatcat cacggttctt
    31801 acctgtcact ctaatctgat cttcttggat ttgtgctttt actttcagtt tttcattttt
    31861 gatagctgta gtgattttct tcgcgttttc acgatcgatt ccgttgacca aatcggcagt
    31921 ttgtcgagct ttgccaccta gagcgtgttc tgattcggaa aaatggatat ttttgattgg
    31981 cacatttctt ttgatcagct taccaaataa tacatctttg atctgttcga ttttgaaatc
    32041 atcatcgctc aatacaacta gattgctgtt ctctaatttg atttctactg tagaatcctt
    32101 gaagtcaaag cgattggaca attcttttgt tgtttgctga atcgcatttt ttacttcttc
    32161 tgtattgact tctgatacaa tgtcgaagct agcatctttt gccataggaa ccctcctgat
    32221 atacatataa atatttacca tacatctatc ttatcatagg attagggcag attcttacct
    32281 gtttgctaaa attcttctca tttgagttcg aagtatttta ttttaataga taaactgtta
    32341 ttcttacatt tggaatacca aagtagggga gaggtgcttg tgagtaaaat tatgactgcg
    32401 tatcataaaa atgaaagatt aaagaaaata gtggttattt tgataaccgg attgaccgct
    32461 gcggtcgctt taaattattt tttgattcca gccaatgtct tttctgcagg gatgaatggg
    32521 atcgctcaaa tcatttcctc gcttttatat gaatggtttc atatcaagat agacacagga
    32581 acatttattt ttcttttgaa tatccctgtg tttgtattag ggtttctcaa agtaggaaaa
    32641 cgagcaacga tccttagttt tgtgaatgtt gtatgtgtat cggtgatgac gatgctgctt
    32701 ccagtcggac agatcactga taatattttg atgaatgccc tagtaggcgg tgttttatta
    32761 ggtcttggcg taggattatc tttaaaaatg ggctttacaa ctggtgggat ggatatcatc
    32821 tctcttgtac tttcacaaac aactggaaaa acagtcggaa actacatgct gattctaaat
    32881 ggtatcattg tagtcatcgc aggcttcttc tttaactggg aaagtgcctt gtatacgatt
    32941 atttccattt atgcaatgac tcaagtaatt gatgccatcc acacgagtca tcaaaaagta
    33001 acagcaatga tcgttaccgt caagccagaa gaggtaacag aagaactagc aaaacggatg
    33061 gtacgtggaa tgacattact gccgtctgtc ggtggctatt caaaaaaaca agggaacatg
    33121 atcatgatgg tcatcacccg ctacgaaatg tatgatcttg atcagatcgt tcatgaaatc
    33181 gacgacgatg catttatcaa tattttacca actcattcag tatttggccg gtttgcaaat
    33241 gaaaacgaac aaaaaatgta tcgaacaact ggcgtattgc cagaaataaa aaatcgttca
    33301 caaaaaaaat gaggaatcag gtttatttta gaataaattt aatctattac atctaataag
    33361 aacattttta taaatttaaa cgagaatctt acttctaaat tctcatttac aagcagctaa
    33421 aaaaagagta tgataaataa aaatcgtgtt aaggagtgtc gtacgatgag cgaagcaaac
    33481 caagaacggt ctggacaaga aatagaacta atcaaagaac gtattcttgc tgccttggaa
    33541 atggtgattg atcctgaatt aggaatcgac atcgtcaatt taggc
//
LOCUS       DANKHL010000005        29458 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000005 DANKHL010000000
VERSION     DANKHL010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.007"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(651..1154)
                     /locus_tag="PW101_000170"
     CDS             complement(651..1154)
                     /locus_tag="PW101_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL2512603.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1513..2538
                     /locus_tag="PW101_000171"
     CDS             1513..2538
                     /locus_tag="PW101_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2512604.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2861..3739
                     /locus_tag="PW101_000172"
     CDS             2861..3739
                     /locus_tag="PW101_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HDL2512605.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4215..4331
                     /locus_tag="PW101_000173"
     CDS             4215..4331
                     /locus_tag="PW101_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL2512606.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5259..5597
                     /locus_tag="PW101_000174"
     CDS             5259..5597
                     /locus_tag="PW101_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512607.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5691..5921
                     /locus_tag="PW101_000175"
     CDS             5691..5921
                     /locus_tag="PW101_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512608.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            6321..6692
                     /locus_tag="PW101_000176"
     CDS             6321..6692
                     /locus_tag="PW101_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL2512609.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6966..7658)
                     /locus_tag="PW101_000177"
     CDS             complement(6966..7658)
                     /locus_tag="PW101_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2512610.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7661..8446)
                     /locus_tag="PW101_000178"
     CDS             complement(7661..8446)
                     /locus_tag="PW101_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2512611.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8683..10083
                     /gene="lacG"
                     /locus_tag="PW101_000179"
     CDS             8683..10083
                     /gene="lacG"
                     /locus_tag="PW101_000179"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDL2512612.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            10108..10425
                     /locus_tag="PW101_000180"
     CDS             10108..10425
                     /locus_tag="PW101_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2512613.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10438..12111
                     /locus_tag="PW101_000181"
     CDS             10438..12111
                     /locus_tag="PW101_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HDL2512614.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            12125..13756
                     /locus_tag="PW101_000182"
     CDS             12125..13756
                     /locus_tag="PW101_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HDL2512615.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(14247..15812)
                     /gene="guaA"
                     /locus_tag="PW101_000183"
     CDS             complement(14247..15812)
                     /gene="guaA"
                     /locus_tag="PW101_000183"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL2512616.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15871..16794)
                     /locus_tag="PW101_000184"
     CDS             complement(15871..16794)
                     /locus_tag="PW101_000184"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDL2512617.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            16990..17595
                     /locus_tag="PW101_000185"
     CDS             16990..17595
                     /locus_tag="PW101_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2512618.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            17903..18919
                     /locus_tag="PW101_000186"
     CDS             17903..18919
                     /locus_tag="PW101_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL2512619.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            19060..20361
                     /locus_tag="PW101_000187"
     CDS             19060..20361
                     /locus_tag="PW101_000187"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDL2512620.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            20407..21069
                     /gene="deoC"
                     /locus_tag="PW101_000188"
     CDS             20407..21069
                     /gene="deoC"
                     /locus_tag="PW101_000188"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL2512621.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            21090..21488
                     /locus_tag="PW101_000189"
     CDS             21090..21488
                     /locus_tag="PW101_000189"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDL2512622.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            21554..22636
                     /locus_tag="PW101_000190"
     CDS             21554..22636
                     /locus_tag="PW101_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2512623.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            22848..24410
                     /locus_tag="PW101_000191"
     CDS             22848..24410
                     /locus_tag="PW101_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2512624.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            24403..25536
                     /locus_tag="PW101_000192"
     CDS             24403..25536
                     /locus_tag="PW101_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2512625.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            25536..26492
                     /locus_tag="PW101_000193"
     CDS             25536..26492
                     /locus_tag="PW101_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2512626.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            26591..27757
                     /gene="deoB"
                     /locus_tag="PW101_000194"
     CDS             26591..27757
                     /gene="deoB"
                     /locus_tag="PW101_000194"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDL2512627.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            27781..28599
                     /locus_tag="PW101_000195"
     CDS             27781..28599
                     /locus_tag="PW101_000195"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL2512628.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            28616..29323
                     /gene="deoD"
                     /locus_tag="PW101_000196"
     CDS             28616..29323
                     /gene="deoD"
                     /locus_tag="PW101_000196"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL2512629.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         9285 a         5116 c         6332 g         8725 t
ORIGIN      
        1 ataaaaataa aaaattagtc aaagaagggc gtatagattt cttttgatag gagatataca
       61 acagaaatga ataaatatta acgtgttttc aaacagtata aaacggtgtg gtttttggaa
      121 taaaaaaata acatatctca ttttacactt ttgttatttt atacttaaaa aataacgaca
      181 cagaaaatac cgtgtctcag atttctctac tacttctaaa aactactgtt tttttccttt
      241 gaaatgcctt caaaaagtag gatcaggcag aatatactgg cagaatcaag gagaagggag
      301 cctaaataag gagaaaaacc atagaaaata taaagtaatt acttataaaa caagcttttt
      361 atagaaaaat aatatacctg agtagggtat aaattaagac ggaattcctt tctagattga
      421 gatattgact aggaagaatg ttaagatttt aagcctttaa gctaaaaaaa aacccttttt
      481 gttaaataag gggaatatag cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat
      541 atagtagatt agagatgaaa atagattttt ttaaaccaat gactgaataa aaagaccgtc
      601 atagatacac attggaatgt gtctattacg gtcttttttt acgaacgatt ttattgaaga
      661 cggacagttc gggtcaaaaa taaaaaagct acgatcagaa ataagaataa aatgtatccc
      721 aaaataatgg ctagacggtc taacttattt gtcgatagat gtttgtttag aagttgatag
      781 gcccataaac ctatcagtcc tcctgttgta ttagttatca aatcagtgat gtccgctacg
      841 ccaataccga ataggaattg aatcaattct gccaagaagc ttacaaggaa aataatccct
      901 aattttttag aaaaagaaag tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa
      961 aagacaatca agttataagt ggtctcacgt aataccgtcg tattttcaaa agaaaaagta
     1021 gaaaaaggga cgagattgac attgctgtaa gaataagtga gaaattgtgg aagattaaat
     1081 gatagcttga ataaaatcaa ccataataga aagaataaat aaatccctaa taaaaatttt
     1141 gatacatacc gcatagacat ctcctccatt taaaaagtat accatcgaaa accgctgaaa
     1201 aatcataaaa aagtatgaag tttaaggacc aacgtagtta tcgtacgaaa attagaagct
     1261 tttttttgtt gggaatccgg tttctttaac aaagctaaga tagcgagttg cttggaaaag
     1321 ttgctatctt ttttctgtca aaacgactcg gaatgttcgt tttttgttct aatttctcca
     1381 agttgtttac tttttgatac taagtttatg aaaaatcaag aaatagttgt tatactaggt
     1441 aatgctgaca aacgggattg gcatttcatc ttggttcagc tttttttcca attactagga
     1501 aggagggata gattgttcaa gacttatttt cgtttcaata aaatacaaga taatcctttt
     1561 cgtatcatga atctgatgct acttatcttt attgtattat ggttaggtta cataaatcat
     1621 gattttttga tttcaagttt ttcatcatta gggatcttta cttttttcta ttatcaaaac
     1681 ataccgatga aatatttgct gaaacgtatg tttctgatcg gttgcggact gttggttgct
     1741 ttcgttttgg ggatcttatc gacttatgtt ttatggcttg aaccatttgc cgtagctttg
     1801 gttgcttttt ccagcagatt cgtgttgcga ttattccata tttctaaacc aggtggcttg
     1861 ttttttgcga tgctttctgc catgggaacg agcatgcagc tgccaatcgc tcaattaccg
     1921 atcgtctcac tttatttttt tatgggtgtc gtttttgccc tgattgcggc agtgattaca
     1981 aaattactgg attcacggcc tgagcagaca attgaaaaag caactttaaa agaaagattt
     2041 cacgaagagc ctttagtgat cattgattct gttttctaca gcgctgcctt atttctttcc
     2101 gtttatgtca gtcacggctt gaatctgcat aatccttatt ggttgacctt gtcatgtgca
     2161 tcgattttgc tagcagaaaa cttggatgcg atgaagcatc gacaagtcca atatctgatt
     2221 ggatcgatgg gtgggttatg tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg
     2281 cagaccattt ttctgatcac ttttctatat ggaatcgcac aatttctagt tgctagaaat
     2341 tatgcagttg ctaatatttt tcttaatccg atggcgttga tgctatctac acttattcgt
     2401 ggagcatact tgatttcttt gatcgaatat cgttttttag ggatcgtcat aggaagtttc
     2461 atcggtttag gtgttgcgtg ggtaatgacg gttggtttgc aacattattt aacggttgtt
     2521 cgtcaaaaat tagaatagaa ggacaaatat atgtgccaaa agaggttatg acaagatttt
     2581 tgtcacaacc tctaattcaa ggtgttcaaa agccagattc tcagcattaa gtcaaattca
     2641 cacaaaaatg tggaaaggaa ctttcaaaaa aattttatat ttattctatc aaaaaatttt
     2701 gtttatgatg gtaactgaat aaactttcct gagatggtca atgctttcct tgaaagtctt
     2761 acagatattc tttcttcata gaaagtttaa gttttatagt tagtgtacaa taaaagtatg
     2821 tttataccat atggtgtccg gataatcagt catttaaaca atgaggttca aagatatgaa
     2881 aaggggaaat ttgatgaaga aaataagcaa tgaaatccaa attctggcag aaactttaac
     2941 tcttcccaaa gtcgttatag agcaaatgat tgtacaagca aaaaacatcc ttgatgaggt
     3001 gtttgagcaa tatagtcatg aactaatgga tcctaataaa gcaaaggcga ctgcaaagaa
     3061 aatagacaaa gtctatgaag aggaaccttt tgcagcatta gctatttatc tatatgcagc
     3121 ttctcaaagt tggaaaaaaa tttatcaacc attgaagatt ccaagaacta tctatttggc
     3181 aacaatgaat gcttttacta gatttattca tgaacatttc caagtaacag gaagctatcg
     3241 ctttgaccgt ggtttttgga tttggcatta cactagtggc ttgatctttc ggattggtga
     3301 attggagtac gaaatgacat attttccgaa agggtacacg gatctaagat tagaaggaaa
     3361 gcagtgcctt tctcttcata ttccttccga tgcagattta tctcagaata agataagaga
     3421 tagttacgaa agtgccattc gtttttttga actttatttt cctgattacc attaccaagt
     3481 gatgtacaca gatacttggt tactatcacc caatcttact aaatggttga aaaaagagtc
     3541 aaaaatctgt ctttttgcag ccgattatcg attattatca gttgatgaac aggacgatag
     3601 tggagtccct tggatctttg gacgagtaga tgcccaaata catgattatc cagaaagtac
     3661 gagtttacag cgacaagcaa aagagcaatt gcttgcaggc gagcacattg gctctggctt
     3721 aggtattctt acttcataag tttagtactt cgaaatagtt taaaagagcg tgagacaaaa
     3781 gtaaagaaca cttttgtctc acgctctaaa cacgtataaa cggcggaagc agaagcaact
     3841 ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct
     3901 tatttcttgg agttaaacgc ttctgtccca gcctcttttt tagtgttcta tcgaacacac
     3961 agtacgttca ataggtaaac tagctataac aatagatatt aaagttaacg gacaagaggg
     4021 ccttttttag gtagctaaaa cgtaataatg aaaatttatt tccagagata ggagtcaatc
     4081 agctagtctg atcgactgtt ttaacaagtt gactttcatt ttaacttttg ataccattta
     4141 aaacacaaat cgtaattgtt tcgatttgtg ttttttagca tgactacaat caaagaagaa
     4201 agtgggtcgt ccatatggaa aaaacagata tctcaagtgc ataccgccgc ttgaaaagcc
     4261 caaatatcaa aacacgaaaa cgtgcgctga agatcatcaa agatgtgaaa cggaatagtg
     4321 ggaaaaggta agaagaagga aacttaatta aaaaaatagc cgaaaaacgt ttttcggcta
     4381 ttttcaataa tcggtttttg tgtaaggttt ccgaaagaac tgctgttgat tttccatttt
     4441 taacatatga taaattggta gaatctttct tttcaaattg ataccattcc ctaattccag
     4501 ttatatagtt aactacagaa ccacaaagca cgtacgttta taaaagtatt atctgtagtt
     4561 atttttcaat tccctttcaa cctcttattt tgaccagtag tattgatcac tttacctttt
     4621 aacacctttg ttcgtttttg aaacattttt agtcaagaat ggaaacgtaa ctacataatc
     4681 aaaggttttg cgaaatattt catgaaatat ttcatgtaga agcgagcaat acggttgcaa
     4741 ataaccatta tcgtttaata agcagttacg aaagctccca atatctctaa cgttcagctc
     4801 actccatttc ttttcttcaa aaaaatcagt gattcgtatc tctttctatt tgtcgaaaaa
     4861 gttaacttta aaaagtaacg ccgaaaaaat atgcaaaaaa ataaaaaagc agttactatt
     4921 gctagaataa catcaaggaa taataagttt tggttttcag ataatagtcg ataagttgtg
     4981 tattggaatt ttagatacag taataaagta tttttcatga aattttgtga gaatcactat
     5041 tacaagtatc tttaataaaa ttaatggtta aggaggaact taaagtagaa tgtaaatgaa
     5101 tagttgtagt cagtcgattg tgatactaga ctcaaagttg caggaataag tttttcaatt
     5161 tgtgattgga agataaaaaa tgaaaaagaa gggctttttt ttatactttg ttgattgtag
     5221 tgtttatttt ccctctgttt agccaagtaa cattttctat gactcaacca gtttcaagtg
     5281 aggaacatga taaccttttt cgtgctacag aagagtttaa gaaatatcag gaaaatattg
     5341 ttccggagac tccattagct agtgagccaa ttactgatat tgatggaaac caaataggat
     5401 ggtatataca gtacgaagtt atggtggtaa ataatgactc aatagtaaaa aatacagtag
     5461 gcttagagct aacaccgatt gaaaaggtga gttcattgtt aacgtatatt tatactgatg
     5521 aaagtgacga gactgagatc attttaacgg attactcaaa aattgctact gataaaaagt
     5581 atatattgtg gatgtagaca ctagtgaaat actttatgat atagaaattg atgaaaattt
     5641 actagatgta gcacaagaaa tcgatcatca aaagcaggaa gctattcgat atgctggtag
     5701 aaaagggaaa agaaaatatt tattacgtga atgtagcaaa agttagagaa gatgaaaatg
     5761 agtggaaaga attcaagagt cgctactcaa ttaactcgac tccgacattt acagtttaca
     5821 gagaaggatc gatagaaaaa actgtttttt ggactaaaga aagtggaatg tctttagctg
     5881 aagtagaaga gtttttggat tatgttagta tgcaacagta agggagttct tattatgaaa
     5941 aattttgaca catattatgt tattgttttt tcgatatctt tgctgttaat attactaact
     6001 ttaatgcagt ataaagagta tccagagtat tttattttaa cttttgtata tttagtaagt
     6061 agctacacac ttctatataa attcagaagt tcttctagag tttctaaagt ttgtttctta
     6121 attagcttat tgattctata atatttttga tttatatagg aaattaaaaa tattatagaa
     6181 tcaacactca gagaacgggt tggtttttaa tagatttaat aagtactgat aatgaaattt
     6241 ctcctggttg ggaaaacgaa gttggttacc ctgtagaaga attttttcaa gattactttg
     6301 cggagtatgc cgataggata atgagagatt acgtaacaga taaaactcaa gaagtcatca
     6361 atcaatccca gtcattaaat gaattatctg ataaggcgaa agaagaagca gttgccatta
     6421 tttcttcagg gattagtgac tatattgata tgagtggtga aatcaattat ggcgataaag
     6481 acaaaattgt acgcatgata aaagctttta tcaaaacttt tgatgtgaag gcagatctta
     6541 agtttaatga tacttaccta gtaggaaaat taattaatat ccttgggaca gagaaagata
     6601 cggaaacgtt tatttcctct ttgtctatgt tgtttacaga taagcatttt tcaactcagc
     6661 ttggtggaga ggaactagtg gaaatctttt aaccattacg cccagcatta ataaaagtat
     6721 atcaaaaaag tactcctgat gatttttaag attggtttga gaaataatgt gaaaacacta
     6781 tttttaatgt taattataga tagagatgaa cgcgtcatat ggttaaaaga atgaaaaagt
     6841 caaagataca ctgctatatt tcactaagga tatagcagtg tatctttcat gtcgtttcac
     6901 tggcagtttt ttataaaagg aggtgtgcat ttcagttgct tttctttggt agttagttga
     6961 atcgtttaag aattaacgga tgtggcagac aggtgctctg tccaaaaacc agtaaaatct
     7021 ttgatggttc catctttatt tagctgtgcc tgttcaaata ctgttttgtt tttaccgaat
     7081 ggatcaattg caataattgt tccgatcccc gcacgtttgg cagcaagtaa tcctgagtaa
     7141 gcatcttcga taacaagaca atcagccgga gctaagctca gtttgtctgc cgctttaaga
     7201 aaaatatccg gttgcggttt cccagggaaa gagccgtcgt catagacaat cttgtcccag
     7261 tcaaaccagc gggctaaatc gaaaatatca aaataaaaag caacattctc tttgacagta
     7321 gccgtggcaa tcgttaatgg aatctgttgg tcagtcagct ggtcaagagt cgcttcaagc
     7381 ccttttgtca actgaagctc gttttcattt tgcaagcaaa gcatacgata atagctttct
     7441 ttttcttctg acaatacggc gatctcagag gcggtcaaat gctctgaaat gaaatgtttc
     7501 aaaatctccg tattcgtccg accatgaata ttttttaaaa tatcctcgtc tgataaacca
     7561 tcttttgtat gatcatgtat catatgcagc caagcagctt catgcagatg agagtccaaa
     7621 aacattgttc cgttgaaatc aaatataatc cctttcatag ttacgctcct atagtggtat
     7681 tttcggttga atcagcaatt tgcagatcga catattgtgc atagtgctct tgaacctccc
     7741 gcgagatctg gcgatccgta atgagcagat cgaaatcatg caaattataa aacacataaa
     7801 aatcatctcg attgaatttt ttattatcaa cgagtagata tttcttacgg gaattattta
     7861 gagcgatctg ctgtgcttct ccttcttcgg tactgtaagt agagatcgct tcgttgtgaa
     7921 cgccatttga actgataaat gcttttgtaa actttagtct tcttaaattg tcgttggtga
     7981 tcggtccgac aaatgcgccg gtatttttcc ggaaatcacc gccaatcagt agaatttctg
     8041 gtccatctgc ttgagcaaga atatcaaaaa ctggataatt atttgtaatg atccgaattt
     8101 tcttgttcag taaatgcgtc gccaatagtt cgattgttgt acctggtcct aagaaaatcg
     8161 tttctccctc gtgaatcaag gaggctgctg tggcagcaat ttcttttttt tcttgaatct
     8221 gcagagtggc tttttcagtg tgagaaagtt cttgatctaa agtgtaggaa agactttgag
     8281 cccctccatg gacacgcagc agcttgcccg atttttctaa ttcatctaaa tctctgcgaa
     8341 cggtcatatc agaaacatct aattgattga tgatttcatt aacggtaagg attccttttt
     8401 tattgaccat ttctacgata gtaagcaatc tttctttttt caacattgtc ttcacctcta
     8461 atttaaggat aatctatcat ataaacggac aacagtcaat ctaattcgaa caaaagtaaa
     8521 catttagggt ggtttttatg tttgttacaa tagattttat tgctgttttc tggtttttat
     8581 gattatttgt gtttgttttt gttgacaaat gttttggatg cgtttacaat gcaatcagtt
     8641 agaaagagcg aaaactgatt atagatttga ggagagatgt acatgcttaa attaccagaa
     8701 gattttattt ttggcggagc cacagcagcc tatcaggttg aaggagccac taaggaaggt
     8761 ggcaaaggcg ctgttgcttg ggatgacttt ttagaggaac aagggcgatt cagtcccgat
     8821 ccggcaagtg atttttatca tcaatatgca aaggacattg aattgtgtga acgttttggt
     8881 gtaaatggtc taagattatc cattgcttgg agccgcattt ttcctgatgg agctgggaaa
     8941 ccaaatccag aaggaatcgc tttttatcat cgtgtgttcg aagaatgtaa gaaaagaaac
     9001 gtaacgccgt ttgtgacgtt gcatcatttt gataccccta aacgtttgtt tgaccatgga
     9061 gattttttga atcgagaaac aatagaagca tttgtttcct atgccatttt ttgttttcat
     9121 gaattcaaag aagtcaaggt gtggagtacc tttaatgaaa tctatccagt tgcaaccaat
     9181 caatatttgt taggtgtttt cccaccagga atcaaatatg attttacaaa gatcgtcgcc
     9241 tgtcttcata atatgatggt tgctcatgcc cgtgtagtga actatttcaa agaaaacgaa
     9301 cttccgggtg aaattggtgt agttcactca ttagagacga aatatgcggc aacagacgcg
     9361 ccggaagata agcatgctgc ctttttagac gatgcattgt cgatccgttt cttattagat
     9421 gctacctatt taggctatta ttcaactgaa accttgactg ctcttgatga gatatgtgaa
     9481 gcaaatcagg ccagctatca ttttcctgaa gaagatttcg ttgaattgaa aaaagcatcc
     9541 acgcgtaatg actacttagg gatcaatcac taccaatgcc attttgtcaa agcttacgat
     9601 ggagaaaatg caatccatca taacggtacg ggtgaaaaag ggacctctgt ttataaagtg
     9661 aagggaatcg gagaacgtat ttataaagaa ggaattcctc gaactgattg ggactggctg
     9721 atttatccag aaggtctata tgacttgttg ctgcgaatta aaagcgatta cccacattac
     9781 aacaaaatct atatcactga gaacgggatg gggtataaag atcagtttga agatggcatt
     9841 attatggatc agccgagaat cgactatttg agagtctatt tggaatcctt aagcaaggcg
     9901 ataacagccg gggtaaatgt gaaggggtat tttttgtggt cgcttatgga tcttttctca
     9961 tggacaaatg ggtataacaa aaggtatggc ttgttttatg ttgattttga aacccagaag
    10021 cgttacccaa aagaatcagc ttactggtac aagttggtca gtgaaaccaa aacgattatt
    10081 taataagtga taatggagga agaaaagatg aataaagagg aattacaaat gcttggtttt
    10141 gagatcgttg catattcagg cgacgctcgc agtacattat tgaaattatt gaaagaagta
    10201 agacaaggaa actttgaaca tgttgattcg gcgttgaaag aagccgacga aaatttaacc
    10261 cttgcacata attcgcaaac aaaaatttta gcggaagaag catctgggaa agagatggag
    10321 atgggattca tttttatcca tgggcaggat catttaatga ccactttatt gctgagagat
    10381 ctgattcaag actttattgt actgtacaga cagaatcaag gataggagaa gaacgctatg
    10441 gatggaatca ttaaacaaat cgaaaaaggg aaaccatttt ttgaaaaaat ctcacgtaat
    10501 agttatttag gagccgttcg tgacggattt ttaacagcga tgccagctat tttattttca
    10561 agcattttca ttttgattgc tgcggttcct gagatttttg gttaccaatg gcctgaatct
    10621 gtctccacat ggctatggaa agtctataat tattcgatgg gtgttgtagg gttactcgtt
    10681 tcagcaacca ctgctcgctg cttggctgat tcgatgaatc gtcgcttacc gaaaaacaaa
    10741 gtaatcaaca ccacctctgt gatgctggcc tctgtggtaa gtttcctgtt gttaagcgtc
    10801 tctccgattg aaggtggatt ttcaacggaa tatatgggga caaagggact actggcttcc
    10861 tttgtcgctg catttttaac agttaattta tataagtttt gtgtgctgaa agatattacc
    10921 attaaaatgc ctaaagaagt tcctggaacg atttcgcaaa ccttccgaga tatttttcct
    10981 ttctcttttt cagtatttgc ggccgtttta attgatttag gcattcgaca aatcttccaa
    11041 atctcatttg cagaagcaat cattacggtg ctgcagccgc tatttacagc agctgacggg
    11101 tacttaggaa tcgctattat ctggggagcg atggcgctgt tttggtttgt cggtgttcat
    11161 ggaccgtcga tcgtggagcc ggcaattgcg gcaattatct atgccaatgt ggaaaccaat
    11221 ctgcaattgt tcaattccgg tgaacaagct tctaaagtac tgacggtggg tttagggaat
    11281 tttgtaggaa ctatgggagg aactggggca acattggttg ttccttttat cttcctactc
    11341 tttgctaagt ctaagcagtt gaaggcagtt gggaaagctt cctttattcc agtaagtttc
    11401 gctgtcaacg aaccattgct atttgcggca cccatgattt tgaaccctta tttttttatt
    11461 ccatttttgt taacacctgt tgtaaacgtt tgcctgttca agttttttgt cgatgtattg
    11521 ggcatgaaca gctttatgta cgttttgcct tgggcaactc cagcaccgat cggtttggtt
    11581 ctaggaacgg gcatgggtat cttagctttt gttctagcag cggttctaat cgtcgttgac
    11641 ttcctgatct atctaccatt ttgcaaagca tacgatgctg tcttagtaga gaaagaagca
    11701 caaaaagcag aggtgatcgc aaccgaggaa ggtgcttctg ccgatgtacg tgaggaagta
    11761 cctgtggcag cgagtcaagt tatcagcgat gcaaataaag aaatcaatgt gttggtactt
    11821 tgtgctggcg cagggacaag tgcgatgtta gctaatgctt taaaaaaagg tgcatctgaa
    11881 tcgaaagcac cgatcagtgc aatggcgggt gcttatggtt ctcattacga aatgctgcag
    11941 gattttgata tggtggtctt agcaccgcaa gtcaattctt actatgatga tatcaaagaa
    12001 gacaccgatc gcttaggaat caagctggtt gcgacaaaag gagcagaata tatcggatta
    12061 acgagagatc ctgaaaaagc tgttcagttt attctgaacc aatttcaata aaggaggagc
    12121 cataatgaaa atgggaattt ggttgcgtaa agctgtttta tttgcagctg tgttaggggc
    12181 tttgggggct gtttcaaaaa ctgttgaagc atctgccagc gaatttattc gcggagcaga
    12241 tatttcgatc ctgtcagata tggaaaaatc tggtgcaaaa tattatgaag acgatatttc
    12301 aaaagatgcc ttagagattt taaaaaataa tggtgtcaac tatgttcgac tgcgtttgtg
    12361 gcaggatcct tataatagta atggagaaag ttatggagca gggacaaatg atctggaaac
    12421 gacgattgac cttgcaaaac gagcaaaaaa tctgggactt aaggtgctgc tggattttca
    12481 ctacagtgat ttttgggttg atcctgggaa acagaatctt cctaaagcat ggcaaggtct
    12541 gacctttgaa gaaatgaata cggcattgta cgactataca aagaacgttc tgtcggaaat
    12601 gaagcagctg gacgtttatc ctgatatggt tcagatcggc aatgaactga atagcggaat
    12661 gctctggcct tatggcaagt cttggggaga aggcggggga gaatttgatc gattggctgc
    12721 ttttttgaag tctggtatcc aagctgttcg agacactcaa cctaaaacaa ctccagtaat
    12781 gctccactta gctgatggtg gcgatacagg agcatttact tggtggtttg atgaaatcac
    12841 gagtcgaggt gtgtcatttg atttaattgg agtgtcgtat tatccgtatt ggcacggttc
    12901 aatgaatgaa cttcaaacca atatgaatgc gatcagccaa cggtatggta aagaagtgat
    12961 cgtagttgaa acagcttatg ggcatacaac agcgaatgcc gatactatgc caaatgcttt
    13021 tggtgaagcc gaagcagctg cggggggata tgaaccaaca ccgacaggac aagcagagta
    13081 tttgctggat cttgccgatc ggattcaggc agtaccgaat aatcgcgggg cgggcttctt
    13141 ctattgggaa cctctttggt acaacggaaa tgtgagttgg gcaacacaag caggtatgaa
    13201 ttatctcagt gttcaatcca ctatgggaaa cgaatgggac aaccaagcga tgtttgattt
    13261 tgctggaaac gccttgccaa gcttgcgtgc gtttaaacaa gcaggggcgc aaataaatct
    13321 agttaaaaat gcaagtttcg aaagcgatgg accaacgacc agtccttcca attggcaaac
    13381 ctggtttcaa caaggaaccg ctaagggtac gatcaaaacg gaagcgggca ctttttttgg
    13441 ggactacaaa ctaacttttt ggcaggatca agcatacgag gcatcagctt atcaaaatgt
    13501 tagtggcata agcaaaggaa cttacacatt gagtgcctat gtaatgtctg ggggagagtt
    13561 tgatactaat cagttatata tcaaaaatta tggtggagcg gaactaaata ctgcggttgt
    13621 taaaacaagt gaatggacaa aaattacaat caaagacatt ccaattacaa caggtgtttg
    13681 tgaaattggg gtttatagca aggcttcagg aaatgcttgg acgaacgttg attttgtttc
    13741 attggtgaaa caatagactg cggatcgttt cgattcaaga tcggaggttc ccttcagcca
    13801 catcgttagc gtagaaggca ggaatcgaac ctgcacctcg cctttgccaa aaataaccag
    13861 aaacgatttc cagtcatttt taccaaaacg agtaaagcac cagacttcat ctacactttg
    13921 tattatgcta aagagagttc aacgatttcg taagaagcaa tgaaaaaatt gcaaaaagtt
    13981 gtgcagataa cataaagttt gtttaagaaa gggtaacctt agaattccgt cgtgaatatg
    14041 atttgagcac ttagatggat agaaaaaagt tctcggaaaa cagttcgttt ttcgagaact
    14101 tctttttatg ataagaatga aacaaattaa cttatattat agtctctttt ggtagggaac
    14161 catcttattt ttaatgaaaa tcaagcattt tagctattga agaatgttga tttataggga
    14221 ttccccctcc atattatgtt ggctctttat tcccactcca ctgttgctgg tggtttagaa
    14281 gtgatgtcat acacgatacg gttgacgtgg gctacttcgt ttacgatacg tacagagatt
    14341 ttttgcaaga cttcccatgg gatacgggca aagtcagcgg tcattccgtc gattgaagtt
    14401 acagcacgga taccgactgt gtagtcgtaa gtacgtccgt cacccataac accgacagag
    14461 cggatccctg gaagaacagt gaaatattgc cagatatcac ggtcaagtcc agcagctgcg
    14521 atttcttcac gcaagatagc atcagattcg cgtacgattt ctagcttgtc ttcagtgatt
    14581 tcgccaagaa cacgaatacc aagaccagga ccagggaatg gctgacgcca aacgattgaa
    14641 tcaggcatgc ctaactgagt ccctaattca cggacttcgt ctttgaataa tgtgtttaat
    14701 ggttcgatca attcaaattg catatcttct ggcagtccac ctacattatg gtgagatttg
    14761 atcgtctgtg ctgtttctgt accagattcg ataacgtcag tatacaacgt tccttgtgct
    14821 aggaaagaga cgccttcttc accagcaagt tttgttgctt cgtcatcaaa aacatatacg
    14881 aattcattcc caatgatttt acgtttttgt tctgggtcag atacaccttt caacttgtct
    14941 aagaagcgat ctcttgcatt gactttgatg atgttcaagc cgaattttcc gcctagactt
    15001 tccattactt gttccgcttc gcctttgcgt agcagaccgt ggtcaacaaa gatagatgtc
    15061 aattggtcac cgatcgcttt ttgtaaaaga accccaacga cactagaatc aacgccacct
    15121 gaaagaccaa gcaagacttt tttgtcacca acttgttcac ggattttttc tacttgcata
    15181 tcgataaagt tatccattgt ccagttacct tcacagtgac aaacatcaaa ggcaaagtgg
    15241 cgcagcaatt cgattccgta ttcggaatga cgtacttctg catggaattg gataccataa
    15301 aaattacgtg ttgtgtcttg gatagaagca atcggacaat ctttgcttgt tccgactgtt
    15361 tcaaagcctt caggaacttt agttaccaag tcaccatggc tcatccatac tgtttgttgt
    15421 tttggtgttt ctttaaacaa gacagcttga tcgcttgtta cttctaacaa ggctttccca
    15481 tattcgcggt ttttagcagg ctctacggaa ccgccaagtt tgtacgtgat caattgcata
    15541 ccatagcaaa tccctaatac aggaatacct aaattgaaga tctcaggatc gatcccaaat
    15601 gcattctcat catatacact gtttggtccg ccagaaagaa tgattccctt tggattcatc
    15661 gcttttactt cttctgcagt aatgcggtgg ctcaataatt cggaaaagac tccaagatca
    15721 cgaatacgtc ttgtgatcaa ttggttatac tgactaccgt agtctaatac gataatcttt
    15781 tcaacgttcg tcatatcggc aacgtttgtc aaatttatta cccctatccc tttaaatttt
    15841 atctcaaaat agtcaaacta ttttaaaatg ctaatatttt cgcatgaaaa tcgaatcgat
    15901 gatatgattt tcagttttat ggagaattat atcggctctt cctcgtgtag gcaagatata
    15961 ttcctgtaaa tttttcaaat tcacattttt ccatacatct tttgccattt taaaggcttc
    16021 ttcacgatct ccgatagcaa aacgataata ataattctct ggattttgga aagccgtatc
    16081 cagtaacgca ccaaaacgat ccaaatacca tttttcgatc agcacgggat cggcatcgac
    16141 atagacagaa aaatcaaaaa agtcgctgac gtagatttgc tgattcgcag gtaattgaag
    16201 cgtattgatc ccctcaacga tcaagatatc aggctgctgg atcaattcgt acttgtctgg
    16261 aatgatatca tacacatcat gtgagtaaac aggtactttt atctcttcct gaccagattt
    16321 tacacggttc aagaaatcga tcagcatctc catatcataa ctttcaggaa atcctttacg
    16381 gtccatgatt ccacgctctt ttaatatacg atttggataa agaaatccgt cagtcgtgat
    16441 cagctgaacg tttcttcgtt tgaaggtacg agacaaaatc gtctgcagca gtctggctgt
    16501 tgtacttttt ccaacggcta cacttccggc gatcccgata ataaaaggcg gtacttctac
    16561 gtatttatga agaaataaac ctttactcaa gaacaaagat tcaaattctt tcatataaag
    16621 ataaatcaga tgagcaagag gtacatagat atctcgaaca tcctgcatgg aaatgcggtc
    16681 attcaaactt ttgatattat ctagttcatc ttgtgtcaaa ggtgcttgcc cattacgata
    16741 aaattctcgc cattcttctc ttgccacacg gtagtagttc attggatcat tcatattagc
    16801 ctcctgcgtt ctcccatcaa tggaactatt ttaacacaaa ataaacgcaa accccaccac
    16861 ttaagagaga ttgccgatct ttttgtataa tctttataat agaaagaaac ggagtcttat
    16921 tcttgacttg cgcacggata cataaaagga tagactgttg tcatgaaaga aatgagaagg
    16981 atggaagaca tgacaaatca ttactattct gaacgcccag atacggcgca cgattttgaa
    17041 caatgggctt ttgagttaaa aggcagaacc tttcaatttg taacagattc tggtgtattt
    17101 tcacgtgaaa cagtggatta tggttcacgt gtcctgattg atgctttcga ttgggaagag
    17161 ttacctgatg aaggaaagat cttagatgtg ggatgcgggt atggaccgat tgggttagct
    17221 ttggcatttg cgacacagcg gtttgttgaa atggtcgaca ttaatgaacg agcggtaagt
    17281 ctagcgcaag gaaacgcaaa gcgtaaccaa atcgaacatg ttgatatcca tcaatcgaat
    17341 atctatgaag cagttcatga agaaacctat gcagctatcg taagcaatcc gccgatccgt
    17401 gctggaaaaa aagttgtaca tgaaattttg acaggcgcat atcctcggtt gaagaagggc
    17461 ggtacactga caattgtgat ccagaagaaa caaggagcgc cgagtgcgca aaagaaaatg
    17521 gaagaaacat ttggtaatgc agaaatcgtc acaaaagata aaggatatta tatcatcaaa
    17581 agtgtgaaag aataactagc tgaaaaatag tggagcgacg aaagagtgga actgggcgtg
    17641 catgtacggg tacgtatcag ttcttttttt gttgagaagt ttttaaaaac aaagaactat
    17701 tttttcttca aaaagaaata gtaggaacag agtcgttgtt ttttacgata aaataaataa
    17761 gatacagaac ttgtatagaa gactgccaag tcggtagata aaggaggttt ttatcaaaaa
    17821 acatactttc cttttttctc tattcttttc ttgggggttt tggtacactg atagggaatt
    17881 tagatagaag ggagccaata ggatgaagct gaccatcaga gatatcgcag aaatggcagg
    17941 cgtatcagta acaactgtct cacagatttt gaataacaaa ggaagcagat ttagtgataa
    18001 gacaagaaaa aaagtgttgt cgatcgtgaa tgaacatcat tataagcccg attatttcgc
    18061 ttcaaatgtg atcaatcgac attcaaagac catcggaatg atcgtccctg atgtcactga
    18121 tttcttcttc tccaaactga tcgaaggtgt agagagctat ttgaatcctt tgggctatgt
    18181 gatcatgtta tgcaattctc gtcatagcca agaaaatgag atcaagtatt tacaagagct
    18241 ttcccaccgc tcagtggacg gaatcttgct ggccacacca aatattttac ctgagaaata
    18301 tgcattagac agcggttttt atcaaaaaat gccaatcatt ttaatcgatc gcggattgaa
    18361 tcgtcgggat aacggacggc ttatcgtaaa ggaatatgag ggcgcctatc aagctgtttc
    18421 gctgtttatt gaaaatggcc atacgaagat cggcatgctg aaggaaacca ctggatacta
    18481 tcaattagaa gaacgtttca acggatatcg tcatgcgctg aaagatcatg gcctgccttt
    18541 taatgggaaa tttgttgaac atggagagtt gactgtccaa ggtggatata ctgcatcaaa
    18601 aaaattactc aaacataaag atatcacggc tattttttgc gggaatgatg ccatggcgat
    18661 cggatgctat caggcgatcg atgaattggg caaaaaaatc ccagaagata tctcagtcat
    18721 aggattcgat ggattaaagc tgtcagaata catgattccg caattgacga cagtgcagca
    18781 gccaagtttt gatattggat tttatgctgc acgtttcctg atcgatacga tcgaatttcc
    18841 tcaaagaaaa gttccgaaca aagtgttcga aacaaaatta attattcgtg agagcgtaaa
    18901 agtattgaca aagggctaac tcggcggtat attgcttatg taagcattta caaaggctag
    18961 ttagtgcttt ttttattttt agtaaaccag tttactaaag tggtttacct ttcctaaaaa
    19021 taatgtataa taccgaatga agacaaggag gatcaggcaa tgcgaatggt ggatctgatt
    19081 gaaaaaaaga gggatggaaa cgagctgtcg aaagaggaaa tcgagtacat cgtcacgaac
    19141 tatacgaatg gaaagattcc agattatcaa gtcagtgcat tattgatggc aattttttat
    19201 caagacatga caaatgaaga aatcacgaac ttaacattgg cgattgctaa ttctggtgat
    19261 gtcatcgatt tgagttctct tgaaggaata aaggtagata agcattcgac aggtggcgtt
    19321 ggtgacacga ccacattgat tttagccccc ctagtagcta gtgtcggagt gacagtggct
    19381 aaaatgtctg gacgcggtct aggttacaca ggtggtacac tagacaagct agaagcaatt
    19441 cccggatttc agatcgagct ttcggatgaa gaatttgtac gcattgttaa tgaaagtaaa
    19501 gtagcggtaa ttggtcaatc aggaaacttg gcaccggcgg acaaaaaatt gtatgccttg
    19561 cgtgacgtca cagcgaccgt tgattctctt ccgctgattg ccagttcgat tatgagtaaa
    19621 aaaatcgcgg caggagcaga tgcaatcgtt ttggatgtga ccactggaga cggtgctttc
    19681 atgaagaaca tcgaagatgc aagacgtctg gcaaagacca tgacgagcat tgggaaactg
    19741 gctaaccgag aaacagtggc ggttatctca gatatgtcag aacctcttgg cgaagctatc
    19801 ggaaatagtc tagaagttgt cgaggcaatc gaaacattac aaggaaatgg tccagaagat
    19861 ctagtagaaa tgtgctacgc cttaggaagc caaatggttg ttcttgctgg caaagctaag
    19921 acaaccgatg aagcaagaac gttgcttcaa gaagctctgg aatcaggtaa agcgttagca
    19981 aaattcaaag aaatgatcca aaatcaaggg ggagacccag cgatcgtgga acatccagaa
    20041 cgaattttga ctgcacgcta cacaatggag cttcctgcta aacaaagcgg agttgtgtca
    20101 aaaatcgtgg caaatgaatt aggaattgct gcaatgatgc taggtgctgg gcgtaaaacc
    20161 aaagaagaag atattgatca cgctgttgga ttgaaactgc ataaaaaaat tggtgacact
    20221 gtgacaaaag gtgagtcact attgacgatc tatagtaacg acgaagaaat cacttctgtc
    20281 attgaattat tatataaaaa tattgaaatt ggtgaatcgg caatgaagcc aacgttgatt
    20341 catgacatca tcacagaata aaggaaaaaa gcggacttta ccgttttaaa gaaaagggga
    20401 aaagaaatgg aactaaaccg aatgatcgac catactattt tgaaagcaga cgcatctaaa
    20461 gaagctgtga tgcaaattat cgaagaagca aaaaaatacc atttttattc tgtctgcatc
    20521 aatccagcat gggttgcgct agcgaaagaa caattacaag gtgagccagt agccgtatgt
    20581 acagtaatcg ggtttcctct aggagcaaac acacccgaga caaaagcatt tgaaacaaca
    20641 gatgccatca ataacggtgc agatgaagtc gatatggtca tcaatatcgg tgctttgaaa
    20701 tcaaaggatg atcaacaagt ccaaaaagat atcgaagcag ttgttgaagc agcaaaagat
    20761 cgtgcgcttg tcaaagttat tattgaaaca tcgttattag atagagaaga aatcatccga
    20821 gcttgcgaaa tagcaaaagc tgcaggcgct gattttgtca aaacatcaac tggtttctca
    20881 acaggcggag caaaggtaga ggatgtccgt ttgatgcgtg aaactgtagg accagaaatg
    20941 ggtgtcaaag cttccggcgg tatccataac gaagaagaag caatggcaat gatcgaagct
    21001 ggtgctacaa gaatcggaac aagcgcagga gtagcaatcg tatctggttc aactggtgaa
    21061 ggctattaat tttttcaaag gagaagctca tggcagaact caaacaagaa tggatcaaca
    21121 cagctattga agcgttagat aaagcctatg ttccttattc ccattttccg gtaggcgctt
    21181 gtctcgtaac ggaatcaggc aagatttatc aaggcatcaa cattgagaat gcatcatttg
    21241 gattaacgaa ttgtgccgag cgcacagctt ttttcaaagc agtatctgaa ggagaaaaaa
    21301 catttacgca tctagtcgta gcggggcaca cccccgatcc tatctctcct tgtggtgcat
    21361 gtcgacaagt aatggcagaa ttctgttccc ctgatatgcc ggttacgcta gtaggagaca
    21421 atggtgtgac gaaggcaacg accgttcgtg cactgctgcc ttatgcattt actgaaaaag
    21481 atctataact catgttcatt cagtcagctg actgttgaaa aataaaacaa ttattttagg
    21541 aggctttaca gaaatgaaaa aagcaaaatt atttggttta ggtgctgtcg cactagcagc
    21601 agggttattt ttaggtgcat gcggcaacaa cggctctact gattcatccg gtggcaaaag
    21661 ctcatcagac acaacaactg cagcattgat cacagacaca ggtggtgtag acgaccgttc
    21721 attcaaccag tcagcttggg aaggactaga aaaatggggg aaagatcaag gactttcacg
    21781 aggaaatgat ggcttccaat atttccaatc ttcaaatgaa tcagattaca tcccaaatat
    21841 tgaccaagca ttgaatgctg gattcaaaac gatttttggt attggttata aactaaaacc
    21901 agcgatccaa gaacaagcaa caaacaatac aggtacgaac tttgttatta tcgatgatgt
    21961 catcgatgga ttagataacg ttgtatctgc aactttcaaa gacaacgaag cttcttatct
    22021 tgcaggtgtt gcagcagctt atacgactga aacaaacgta gtcggcttta tcggcggtgt
    22081 aaaaggtgaa gtaatcgatc gttttgatgc tggattcaaa gccggtgttg atgcaggtgc
    22141 gaaagaatta ggaaaagaaa tcaaagtatt gaatcaatat gctggtgact tctctgctcc
    22201 agataaagga cgttcaattg ctcaaggtat gtatgcacaa aacgcagata tcatcttcca
    22261 tgcttcaggc ggtacaggta acggtgtatt ccaagaagca aaatcattga acgaatcagg
    22321 cgacaaaaaa gtttgggtta tcggtgttga ccgcgaccaa tcagacgaag gcgaatacac
    22381 attaaacggc gaaaagaaaa acttcacatt gacatctaca ctgaaagcag ttggaacagt
    22441 agtagaagat ttggcacaaa aatcagcgga cggtaaattc cctggcggcg aacacactgt
    22501 ttatggattg aaagaagacg gtgttggtct aacagaagga caactttctg acgaagcgaa
    22561 aaaagcagtt gatgaagcaa aagaaaaaat catttcaggt gacgtaaaag ttccagaaac
    22621 tcctgaagaa aactaaaacc atttctatag ccttgttaga cacatgtttg acaaggctat
    22681 ggtttgatta gaacaagaat tgttcattta tccaagctgc cttgtgacag tttggataag
    22741 ggaacaactc cctgaagtaa tgtttgtttc aaaagaagaa cgctcactaa gcgtgaatct
    22801 tagtcaatcg ttgccctaga tagaaatcgt taggatgtga aaaaaaggtg gttcaagaaa
    22861 atattgtcat agaaatgcag aatattacca aagaatttgg aacattcaag gcaaatgaca
    22921 acatcaattt acaagtaaag gcaggagaaa ttcatgcgtt gcttggagaa aacggtgctg
    22981 gcaaatctac attgatgaac gtgctttccg gattattaga gccgacatca gggaaaattt
    23041 tgatgcgtgg gaaagaagta aagatcacaa gtccgaccaa agccaaccaa ttagggattg
    23101 ggatggtcca tcagcacttt atgcttgttg atgcctttac tgtaacagaa aacatcgtgt
    23161 tgggaagcga gcctagtcgc gcagggatgc ttgaccataa aaaagcacga aaagagatcc
    23221 aaaaagtttc tgaacagtat ggattgtcag tcaacccaga tgcttacgtt cgtgatattt
    23281 cagttggaat ggaacaacgg gtagaaattt taaaaacact ttaccgagga gcagatgtac
    23341 tgatttttga tgagccgaca gctgtattga cccctcaaga aattgatgaa ttaatcgtga
    23401 tcatgaagga attagtcaaa gaaggcaagt cgatcatttt gattacgcat aagttagatg
    23461 aaatcaaagc agtagctgac cgctgtacag ttatccgccg tggaaaagga atcggtacag
    23521 tcaacgttaa agacgtaacc tcacaacaat tagccgatat gatggttgga agaacggttt
    23581 cattcaaaac gatgaaaaaa gaagcgaagc ctcaagaagt cgttttgtct attgaaaatc
    23641 tagtggtgaa agaaaatcgt ggattagaag ccgtgaaaaa cttgaactta gaggttcgtg
    23701 ctggcgaagt acttggtatc gctggaatcg atggaaacgg gcagtcggag ttgatccaag
    23761 ctttgactgg tttgcgaaag gcagaaagcg gacatatcaa gctaaaaggg gaagacatca
    23821 ctaataaaaa accccgaaaa atcactgaac atggtgtagg acacgtgcca gaagaccgtc
    23881 ataaatacgg gttagtccta gatatgacat tatctgaaaa tatcgcacta caaacctatc
    23941 atcaaaaacc ttacagtaaa aacggcatgc tgaattattc agtaatgaat gaacatgcca
    24001 gagaattgat cgaagaatat gatgttcgaa caacgaatga acttgttccc gcaaaagctt
    24061 tatcaggcgg aaatcagcaa aaagcaatta tcgctcgtga agtcgaccga gatcctgatc
    24121 tgttgattgt tgcaaatcca actcgtggac tggatgtagg tgcgatcgaa tttattcata
    24181 aacgtctgat cgaacaaagg gacaaataca aagcagtgtt actgatcagt ttcgaattag
    24241 aagaaatttt aaatgtttcg gatcgtattg ctgttatcca tgaaggagaa attgtcggta
    24301 tcgttgatcc gaaagaaaca tctgaaaatg aattaggtct attgatggcg ggctatacct
    24361 tagaagaagc acgaaaagag ttagaaaagg gggctggcgc acatgaatga tcgcagcgaa
    24421 aaattccgta atttattagt gcctgtactt tcagttgttt taggattgat cttaggcgcg
    24481 atattgatgg ctgcttttgg ttttcagccc atccaaggtt attcttctat gctagatgca
    24541 gcccttggtt cgcaaagagg gataggtgaa acgttaaggc aagcaacgcc gttgattttt
    24601 acagcattag gtttttctgt agctaactca gctgggtttt tcaatattgg gctttctgga
    24661 caagcacttt gcgggtggat cgtcagtgtc tgggtggcac ttgcgtttcc agatctgcca
    24721 aaagttgttt tgcttccgtt atgcgtgatc ttaggtgcct tagctggtgc gatggcagcg
    24781 gctgttccag gtttacttcg agcatttttt ggaacaagtg aagtgatcgt aacaatcatg
    24841 atgaactata tcttattata cgtaagcaca tttgctttac atgaagtgat gcctgaaagc
    24901 tatcgctcaa gtttggactc ttccaatatg atcagtcaaa atgcctcact gcggttacca
    24961 tggttaacac aagcttttgg cggctctcgc gtaaactcag gattattttt agcaatcatc
    25021 gcgcttattt tcgtttggct gctgatgaag aaaacgactt taggatttga aatccgttct
    25081 gttggattga atccttttgc ttctgagtat gcaggaatga gtagtaaacg tacgatcgtt
    25141 ttgtcgatgg tcatttctgg cgctttggca gggcttggcg gtgtggttga aggattagga
    25201 acgtatcaaa acttctttgt tcaaacgact tcgctatcta ttggatttga cggaatggct
    25261 gtttccttgc ttggttctgg atcagcaatc ggtattcttt tatcagcgat cttgttcagt
    25321 attttgaaga tcggtggact tggcatgcag acaggagcag gtgttccgtt tgagatcgtc
    25381 aatgtatcga ttgctttgat tatcttcttt gtcgggatca acttcctgat tcgtttcgtc
    25441 atggctaaag tcttccaagg gaaaaaacaa gaagagatcg ttgcgacaat agaatcgaaa
    25501 ccaacgaatc aaaaccatga agggggcgaa ctataatgtc gactatcgaa ttaattgcct
    25561 tgatccttac ttctacactg gtttattcaa caccgctaat tctaacgtca ctaggcggaa
    25621 ctttttctga acgaagcggg atcgtaaacg tagggctaga gggaatcatg gtcatgggtg
    25681 cttttagttc catcgttttc aacttgacga tgagtccatc acttggttct atgacaccat
    25741 ggatcgctat tttagtgggt ggcgtcgtag gtatcctatt ttctctgatt cacgcagtag
    25801 caacagtcag tttgcgtgct gaccatatta tcagcgggac agtaattaat ctgatggcac
    25861 ctgctttagg tgttttcttg atcaaagcat ggtatggtaa aggtcaaact gataacatca
    25921 cgcagaatct ggggtatttt tctttcccag gattagcaga tattccggtc attggaccaa
    25981 ttttctttaa aaatacattc ttacctgctt attgtgctat tttgattgct gtcctagctt
    26041 gggtgatcat cttcaagacg aaattcggct tgcgcctccg ttcagttggt gaaaatcctc
    26101 aagctgccga cacattaggt atcaatgtat atgctatgcg ttattcaggc gtactgattt
    26161 ctggcttgtt aggcggtatg ggcggtgctg tttttgctca gtcgatttcc ggtaactttt
    26221 cagctggtac gatcgttgga caagggttca tctccatggc tgctatgatc tttggtaagt
    26281 ggaatccttt aggggcaatg ggtgcctcat tgttctttgg ttttgctcaa agcttgagta
    26341 tcattggcgc acaattacca gttatttctt cgattccgcc agtattgctg caaattgctc
    26401 catatttgct gacaatcatt gtgctggttg ttttcttagg gaaagcttct ggtccaaaag
    26461 caaacgggaa gaactatatc aaatcaaaat aatcttttag cgaataaaaa gggtgggtta
    26521 cgggaagtgt ttttcgtctc gccctttttg ttttaaaggc aataaaaata aaaagaaaga
    26581 aggaataatc atgtttaaac gcgtacatct gatcgtcatg gattcagtag gaattggaga
    26641 agcaccagat gccgataagt tcggtgatgt tggttctgat acattaggac acattgcgaa
    26701 agaagcagga ttgaacgttc cacatttaga gcagcttggt ttaggaacga tccgtcctct
    26761 tgaaggtgtg gaatcagtga aagatcatca aggctatgcg acgaaattag aagaagtttc
    26821 tgtagggaaa gatacgatga caggccactg ggaaatcatg ggattgaata tcaaaactcc
    26881 atttcgtgtg tttcctaatg gattccctga tgatctgatc aaacaaatcg aagaattttc
    26941 tggccgtaaa gtcgtatgta acaaacctta tagcggaaca gctgttattg acgattacgg
    27001 tgaacaccaa atgaaaacag gcgatttaat tgtatatact tctgccgatc ctgtccttca
    27061 gatcgcggca catgaagaaa tcattccttt agaagaatta tatcggattt gccaattcgt
    27121 tcgtgatata acaaaagatg atccgtatat gattggccga atcattgcac gtccgtacgt
    27181 gggtgaacca ggagacttca cacgaacaag caatcgccat gattatgcgt tgaatccatt
    27241 cggacacaca gtccttgatt ctttgaaaga agcaggaaag gacgtgattg ctgttggaaa
    27301 gatcaacgat attttcaacg gacaaggaat cactgaatct gtccgcacga aaagcaatat
    27361 ggatggcgtt gaccagctgc tgaaagttat gaaaaacgat ttttcaggaa tcagctttac
    27421 caatttagta gattttgatg cattatacgg tcatcgtcgg gatgtagtcg gctatgcgca
    27481 tgcgattgaa gaatttgact tacgaattcc agaattgctt gaagcgatgg aagaagacga
    27541 cttgctaatg attacagccg atcacggaaa cgatccgacg tttactggta ccgatcatac
    27601 gcgagaatat gtaccgttat tggtttacag taaaaaaatg aaagaacaag gggcattgcc
    27661 tcaaggatat tattcagata tttcagcaac gattgctgag aactttagtg tttcttcgac
    27721 tgaaaatggc gaaagcttct tgaaattatt aaaataatct ttttaaatag gagaaagcaa
    27781 atgaccaagt taagcgagat gttacaagaa acgacccagt ttatccttga taaaggagta
    27841 aaagaaattg attttggttt gattctaggt tcaggattag gtgaattagc agatgaaatc
    27901 gaagatgctg taatgatttc ttatgatgag atcccgttct ttcctgtttc gacagtagtt
    27961 gggcatgcag gaaagctagt ttatgggtct ctggcaggca agaaagtgtt agccatgcaa
    28021 gggagattcc actactatga aggtcattcc atgcaaacag tgacctatcc agtacgtgtg
    28081 atggccggac taggggccca ttcagtcatt gtgactaatg catgtggtgg tgtcaacgaa
    28141 tcatttgtac caggagattt gatgctgatt accgatcata tcaattttac tggtgaaaat
    28201 cctttgatcg gtccgaatga agatgcaatg ggccctagat ttccagatat gagtcaagct
    28261 tatacgcctg actatcaagc aacagcaaaa gcagcagcag aaaaactagg tttggatctt
    28321 aaagaaggcg tatatatggg ctattctgga ccaacttatg aaacgcctgc ggagatccga
    28381 atgtctcgga caataggagc cgatgcagta gggatgtcga ctgttccaga agtcatcgta
    28441 gcagctcaca gcggattaaa agtgttagga atctcttgta ttaccaactt agcagcaggt
    28501 atgcaagcga atctgagcca tgaagaagtt gtagaaacaa ctcagagagt caaacaatct
    28561 tttaaagcat tggttaaaga aacattgaca ttattgtaat aggaggaaca ataaaatgag
    28621 tgtgcatatt gaagcgaaag aaggcgagat cgccgataag attttacttc cgggagatcc
    28681 tttgcgagct aaatatattg cggagacttt tttggaagat ccagtttgtt acaatcaggt
    28741 acgcggtatg ttaggcttta ccggcaaata taaaggagaa cgtatttctg ttcaaggaac
    28801 aggaatggga atgccatcag ccaccatcta tgcccatgaa ttgatccagt cgtatggtgt
    28861 aaagaaattg attcgagtag gtacatgcgg tgcattgtca aaagacgtcc atgttcgtga
    28921 tttagtacta gcacaaggtg cagcgaccag ttcttcgatg atcgaaaaaa atttccaagc
    28981 ctttcatttt cctccaatca gtgatttcaa tctattactg aaagcatatg agatagctaa
    29041 agaaaagggc tatacggttc atgtaggaaa tgttctttcg gaagattctt tctacaaaga
    29101 tgatttgaca gagacgttcc aattagctga acttggcgtt ttaggagtgg aaatggaagc
    29161 agcggctctt tattacttag gcgcgaaata ccatgtgcag acattaagtt tgatgaccgt
    29221 tagcgaccat ttgatcaccg gtgaagaaac aacagctgca gaacgtcaat ctacgttcaa
    29281 tgaaatgatc gaagtaggat tggaaacagc gatagcagaa taagaaggaa atgaaaaagc
    29341 tgccctgcat taagtaataa gaacatccaa gcaaaaatag ctcctcatgt ttttgacttg
    29401 gctggactta ttattgcaaa tgtaagcttt tgaacaccgt ttaccaagag gttgtgaa
//
LOCUS       DANKHL010000006        29428 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000006 DANKHL010000000
VERSION     DANKHL010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.008"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..364)
                     /locus_tag="PW101_000197"
     CDS             complement(<1..364)
                     /locus_tag="PW101_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL2512630.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRG"
     gene            complement(397..1482)
                     /locus_tag="PW101_000198"
     CDS             complement(397..1482)
                     /locus_tag="PW101_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDL2512631.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            complement(1501..2541)
                     /locus_tag="PW101_000199"
     CDS             complement(1501..2541)
                     /locus_tag="PW101_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2512632.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            complement(2557..3948)
                     /locus_tag="PW101_000200"
     CDS             complement(2557..3948)
                     /locus_tag="PW101_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL2512633.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            4326..5309
                     /locus_tag="PW101_000201"
     CDS             4326..5309
                     /locus_tag="PW101_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDL2512634.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            complement(5677..7815)
                     /locus_tag="PW101_000202"
     CDS             complement(5677..7815)
                     /locus_tag="PW101_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2512635.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(7854..9440)
                     /locus_tag="PW101_000203"
     CDS             complement(7854..9440)
                     /locus_tag="PW101_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2512636.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            complement(9430..10650)
                     /locus_tag="PW101_000204"
     CDS             complement(9430..10650)
                     /locus_tag="PW101_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2512637.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            complement(10662..11468)
                     /locus_tag="PW101_000205"
     CDS             complement(10662..11468)
                     /locus_tag="PW101_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2512638.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            complement(11678..12958)
                     /locus_tag="PW101_000206"
     CDS             complement(11678..12958)
                     /locus_tag="PW101_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HDL2512639.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            complement(13002..15035)
                     /locus_tag="PW101_000207"
     CDS             complement(13002..15035)
                     /locus_tag="PW101_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512640.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            complement(15069..15920)
                     /gene="rfbD"
                     /locus_tag="PW101_000208"
     CDS             complement(15069..15920)
                     /gene="rfbD"
                     /locus_tag="PW101_000208"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDL2512641.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            complement(16018..17046)
                     /gene="rfbB"
                     /locus_tag="PW101_000209"
     CDS             complement(16018..17046)
                     /gene="rfbB"
                     /locus_tag="PW101_000209"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDL2512642.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            complement(17068..17640)
                     /gene="rfbC"
                     /locus_tag="PW101_000210"
     CDS             complement(17068..17640)
                     /gene="rfbC"
                     /locus_tag="PW101_000210"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDL2512643.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            complement(17654..18520)
                     /gene="rfbA"
                     /locus_tag="PW101_000211"
     CDS             complement(17654..18520)
                     /gene="rfbA"
                     /locus_tag="PW101_000211"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDL2512644.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            complement(18620..19354)
                     /locus_tag="PW101_000212"
     CDS             complement(18620..19354)
                     /locus_tag="PW101_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2512645.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            complement(19332..20159)
                     /locus_tag="PW101_000213"
     CDS             complement(19332..20159)
                     /locus_tag="PW101_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2512646.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            complement(20159..20959)
                     /locus_tag="PW101_000214"
     CDS             complement(20159..20959)
                     /locus_tag="PW101_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2512647.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            complement(20952..22091)
                     /locus_tag="PW101_000215"
     CDS             complement(20952..22091)
                     /locus_tag="PW101_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDL2512648.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            complement(22296..23489)
                     /locus_tag="PW101_000216"
     CDS             complement(22296..23489)
                     /locus_tag="PW101_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2512649.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(23568..23774)
                     /locus_tag="PW101_000217"
     CDS             complement(23568..23774)
                     /locus_tag="PW101_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDL2512650.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(23767..24909)
                     /locus_tag="PW101_000218"
     CDS             complement(23767..24909)
                     /locus_tag="PW101_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL2512651.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            25421..26056
                     /locus_tag="PW101_000219"
     CDS             25421..26056
                     /locus_tag="PW101_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2512652.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            26398..27033
                     /locus_tag="PW101_000220"
     CDS             26398..27033
                     /locus_tag="PW101_000220"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2512653.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            27140..28378
                     /locus_tag="PW101_000221"
     CDS             27140..28378
                     /locus_tag="PW101_000221"
                     /inference="COORDINATES: protein motif:HMM:NF017760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2512654.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            28605..29024
                     /locus_tag="PW101_000222"
     CDS             28605..29024
                     /locus_tag="PW101_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2512655.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            29029..29268
                     /locus_tag="PW101_000223"
     CDS             29029..29268
                     /locus_tag="PW101_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2512656.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
BASE COUNT         9014 a         5801 c         4971 g         9642 t
ORIGIN      
        1 ttccacgaat aaactccact gaactattct ttttaaaata attataaata tactcattat
       61 ctttgattgg ataatcagca ccagataata atacatattt taaatatttc tcattttgta
      121 atgctctctt tactaaattg atttctgctt gtacaatgga aaaaccaccc caataaattt
      181 taacacggtt tttattacat aaaaattcaa cattatcata tgatttaaat gcttcaataa
      241 atggttctat atcagttttt ttatctatat gaataataaa aactgcattt tcgttcaatc
      301 gattgaccaa tctctttaag ttgccaatat cattgtatgc caatatcaaa taacatattt
      361 tcaaaaataa tcactcctaa aatatcttga acatttttaa tgatctctac tatatatact
      421 ctccatttgt tcagtagtgt acgaaacatc ataactcttt agttcttgtg ttattttatt
      481 agcatacggc tcttttatta ctttttttgc ccattcttgt ggtgtttcat ctaatgatac
      541 aaaatccaca ttatttgtta actttgtatc tttggaaata gtatctgcta agactaattt
      601 taattttgct gcttgtgctt ctaatccaac cattggcaga ccttcataaa gagaaggcat
      661 tacaaaaaca tccatcacag ataataactc gggaatatcg tttctttctc ctaaaaattt
      721 tactcttttc tctatatcca attctactgc tttggctttc agtttatttc taagtgaccc
      781 ttcacctact aaaatcaagt aagaatcaga attcattttt agtatttcta aaaaaatctc
      841 taacaaataa gattgatttt tttgtttctc taatcgtcca atgtttccca caacaaaagt
      901 actcattgag atatccaatt cttttctctt attaacctta gcttctgaat ttttactata
      961 aagctctaaa tcaataccat tatgaactat ctcaaagggg tggttttgaa acagactttc
     1021 accagcttct tgactaacgg caaggtagtc tgttgcattt tgtaacaaca atttggttaa
     1081 aaatttacgc aatacattca ttacaatatt ttgagatttt ccatcttcag ttgcatggct
     1141 atgagcaatt ctctgtctga ttccattctt tttggctaac cataaattaa ttcccccgcc
     1201 aaaataaacc tgattgtgaa ctatagaaaa agattctttt tttagtaact tattcacttc
     1261 tattaaatat ttaaaaatat ttttcttagg attaggtaca acaaaaatac ggcctcctaa
     1321 tctttcaatc tctgaatgat agtcttgaac atctgcataa tcttcataaa tcaaaaaatc
     1381 aaattgatag tttgtacgat caattttacg atataaattc atcatgaaag actctgctcc
     1441 acctaaaccc attcgtccct gaaaatgcaa aattcttttc attatatttt cctccattaa
     1501 ctaatatact cgcttcaaag cagccattaa ccctggcatt ttcacttcaa cgaagcttcc
     1561 aacttttaca gcagataaaa atagtggagc tggcatattg taaagcaact ttgttatttt
     1621 ttttgctgaa gaatccgtct tagcaatttg cttggcttct cggatcactg gatcactcaa
     1681 tatctgcttt gcttcgtctt tcttctcttt tgaagttctt gcagaggtac tataaataga
     1741 aaaaataact gaagttgtcc cttttaaaag aagcgccgcc ataccgtctt tattaatatc
     1801 gtctgttaca gtatttgcta atgaatagaa ttctttaaat gtttcgaact tgttaggtcg
     1861 gtattgatta gtgatcccct tcccaccttc aaattcataa atatattcta cttttgggtt
     1921 catgatcact tgatgacagt attcataata atgtaagtta aatattcgat cctcacaact
     1981 attataagtt ttaaatagaa tttggttatt tttcagaata tcgctacgat agcatttgtt
     2041 ccatacaaca tacatcaact gttcattcat taactgatac atgtctttta taaactcttc
     2101 gtgagttaga tagtgacatt ctgtaactaa gttttctttt tcggaaacga ttcttccctc
     2161 gtccattgta cgaaaaacaa acgaactaac aacaagatcc gcttttggac tcttttctag
     2221 caaatgatca taatctgaca agatcgtttt tactggcgtg tcatctgcat cgaagaataa
     2281 gagatattct ccttttgact gtcttattcc ttcgtttctt gctaatccgg gcccttgatt
     2341 ggtcgtagta atcaattcaa aacgttcatc attttcaatc agttcttgaa tgatttcctg
     2401 tgtttgatct gttgatccat catttactaa aacgacttcg tactcttcat aatccaattc
     2461 ttttagaata tcaatcgttc gaccaatagt tgcttctgca ttgtatactg gtacgattat
     2521 gctgaatttc ggattgttca tttttacgcc tcccaactag cgaaagattt tttcattcgg
     2581 aaaagcaatt tctgccaatg agatcacatt ttcgtcttct tcaagtcctt gagaggacat
     2641 tttatcaaat aggatcttga tggtttgcag caagatcttt aaatcaagca tcaatgaata
     2701 ttttttgata taaagtaaat caaaacgtaa tttactgttg taatcagaga catattttcc
     2761 atagacttgt gcatacccag tgataccagc acgaacatta tgccgtaagt agtaataagg
     2821 gttttctgca ttgaactgat cgacgaagaa aggacgttca ggacgtggtc ctacgatcga
     2881 catatcgcct ttcagcacat tgaagatttg tggtaattca tcaaagcgga cagctcgaat
     2941 aaatttacct acaggcgtta ctcgtgcatc attgctctta gctaaaactg gtccagattt
     3001 tgcttcggct gtggcggtca tcgagcggaa tttatagatt gaaaattcct tttgattttt
     3061 tgtgatccgc acttgtttat agatgattgg tcccggtgat gtcactttga ctaaaattgc
     3121 tgtgaataac ataaatggtg aagcaataac taataaaaca agtgaaacga tgatatcaaa
     3181 caaacgtttg aatagttcat attcaggttg gattctaaat ccagatgctt caataatact
     3241 ttcatcttcg aaattcataa tatttggatt gatcaatgtc aagttttcaa atgttgtatt
     3301 caaaaacaat tttttttcat ttttagtaac taattgatag attttgattt tttcagtttc
     3361 ttcgatatga gaagcaagat agacaatatc gatgtcttta atcatatttt tgatgtttgc
     3421 ataatagtta tcaagaatga ctgcttcgac tttatgtttg ttgtttttct ttgaaccaaa
     3481 attttggatt gctggtgcga catccttttc taacccaata atgacgacct ttttatctcg
     3541 acttacgcgt aaataaagtt tatatacgag aatacgatag atacttaaga aaagcgtacc
     3601 gagaacaaat gaaagtaaga taacaagacg tggaaaagca aaccagcggc ccgcaaaagt
     3661 cactaccatg atccctagtg tcatcaatac ctgaccgatg actgtgacaa acacgatgtc
     3721 actgatcatt cgattataaa aaacatatgc acccaacaaa atgttcaacg caataaaaat
     3781 cactgaaata ataattgctg agtgttggaa agtttctaaa tttcgtaatg ggatctctcc
     3841 acgaaacttc agtaagaaac tactatatat tgctgcattg tatacaataa catccattaa
     3901 tataatgaat atccgtcgtg cagcactcca ttctccattt ttattcatat atacactcct
     3961 aatcacaaaa taatatctct attatcttac catagtcacc tttaaaaacc tagtttataa
     4021 gcaaattgta atggaattta aaaatgtcta ataaaaagct ttatacacct ctaatcaaga
     4081 caaggactct aaattacaaa tctccagatg aatcgatttt cactaatgtt ggtaaattaa
     4141 agaagtcgaa gtatttgctg ctttttttat ccactcataa agtgaacctt tatatttttc
     4201 agagaatcca tcttcccaaa gcgctgtcta gtccataaga atacctgtat tacgtgaaga
     4261 atcgaaaaaa ttgaaatcga tttttaatga cattatgctt atagaagaaa gtgaggtaat
     4321 atcttatgaa taaacaagag ttgcatgatt ttattggttt tcgacaacca aatatatgtc
     4381 aaattgtcgc atataaggat ggggtagaag tttatacaga tgaatggaat ggttataaaa
     4441 aagacgatac ttgtcatatc atgtcagcaa cgaaaagtat tgtatctttg ctgattggca
     4501 ttgctttaga gcaaggatta atcaaaagta tcgatgataa agtattagat tattttccag
     4561 agtataaagt gaaacgcggg gagaaaacta tttacgctat tacaatcaag cacttgctaa
     4621 caatgaaagc accctacaaa tgtaaaggcg atccatggac aaaagtctgt tcaagtgaag
     4681 attggacaaa aacatcacta gatttattgg gaggaagaaa aggtataacc ggcgaattca
     4741 actatcaaac tgtctgttta cacatattaa ctggcttgtt gtatcagaca agtaagctaa
     4801 caactgttga ttttgccaat aagtatctat ttgatcctat agggatacca aaccatgtca
     4861 attattatgc tgaaacagct gaagaacata agcagtttac aatggataag tctcctaaaa
     4921 ccaatgtctg gttttgtgat ccactaaata tcgggacggc aggatatggt ttgtgtttat
     4981 cggcaagtga tctggcaaaa attggtgtat tatgcctaaa taaagggatg tataaaggaa
     5041 aacaaatcgt accacccatg tggataaacg agatgttcaa acctagaacc agtgctttag
     5101 aagacttaca aaatatgaga tatggctatt tatggtgggt attagattct gagaacgaaa
     5161 tatattcagc aattggtaat agtggaaatg ttctttatat tgacgctaag aacaatctgg
     5221 ttatctccat cacttcttat tttaaaccca tgataattga tagagtggct tttgtagagg
     5281 aataccttaa accatttatt ttaaaatagc ctatattaaa ataaaatatc gattctttga
     5341 cagctagggt tcgtgaaact ttggctatga ttttgtatct attggcgctc tgagcatgaa
     5401 taagagcgtg ggacaaaagt aagaaacact tttgtctcat tctttaaaaa cgaataaacg
     5461 gtgggagcag aagcaacttc ttcttaaata agccgaaatt cacaaaaatt tgaaaagcaa
     5521 ttttcgtgaa tttcttctta tttctcggag ttaaacactt ctgtcccaac ctcgacttat
     5581 ttttttgagt cggcaccttc tgcctttttt tgacaaaaaa ctataaaaag ccgttatgtg
     5641 cgagcgaatc acaacataac agctttttat tatagtttaa tctggtctca acgaaaagtc
     5701 gccgattcct tcttttgtta aattatcatt gtaaaatgga tcatgcgcaa tatatgctgg
     5761 ccatttatct tgcatttttt tgatttcacc agcaaaacgt ctttgttttt ctggagtatc
     5821 ttcataacct cttgatttag attcgaaatg gtacaattct gcttgatgtg catagacatt
     5881 gtaacgaccc agttcataaa ctttcaagca caagtcaaca tcgttgaatg ctacttctaa
     5941 tgtttcatca aacccgttaa cagcattgaa gtcagctgcc ttgaccatca tacaagctgc
     6001 agtgactgct aagtaattga cgtcaatgac caatcggccg aaataaccac aatgtgttcg
     6061 atcatagtta ttcaatgcat gccctgcaac accaccgatt cctaataata ctcctgcatg
     6121 ctgcgttgta tcatctggat aaaacagttt tgctcctaca cagccgattc gatcaaactg
     6181 tgcataagaa accatcaccg tcatccaatc tggctcaatc acttctgtat cgttattcaa
     6241 gaataaaaag tatttgccgt ttgctttttc tgctgctaaa ttgttaattc gagaataatt
     6301 gaatggaatg tcaatcaatt ctacgataaa gcggtctttc agctgatgct tgtattctgc
     6361 aaataattct tgcatttttg gatctgtact tccgttatcc gcaataatga tttcataatt
     6421 tgaataagac gttttttcga tgatcgaatc cacacaagtt tttagatctt catagccatt
     6481 cttcgttgga atgatcacac tgaccaaatc ctcttgcaag acgtcataag caatctcata
     6541 aaatccagaa attctgccag gacgtacgct tcctttgatg tttcttctag acagggcatc
     6601 tgtcaatgct ttcactccgg aatcatagat ataacttttg gcttcgccat tacttgcagt
     6661 tgatcctggg atcgtccgcc aatgatacaa gactcggtca atgtggtaaa tatgatctgc
     6721 agggatttgt tctgtcacac gaaggaccaa atcataatct tgagaacctt catagccttt
     6781 acggaaaccg ccaagttctt taacaatgct tgttcgatat acgcctaagt gagagatata
     6841 attatttccc atcaatgtat caggcgacca gtctgctttg aaatgcggat caaagcgatt
     6901 gccatctgca tcgattttat cttcgtcact gtagatcaca tccagttctg gatgtacgtt
     6961 tagcactttt gccacttcat ataatgcaaa tggcggtaat tcatcatcat tatctaagag
     7021 tgctataaat tctccttctg ctatttccaa tgcagaatta gttgccagac tgatgtgtcc
     7081 attttctttt cggaaaacga ctttgattct atcatctttt gcttgatagg attccagaca
     7141 ctttcggata gctggatcag tagaagcatc atcactgata catagttccc aatgatcata
     7201 cgtttgatcc agtaccgaat caatgcattt ttcgagccat ttgatttcaa cgttatagac
     7261 aggcataagt atggaaatca atggacggta cgcaaatcct tgtgcttctt tcctttgctc
     7321 tttcaaatct ggtttctcat gacgactgat ccactctgca tagtcgatcg atcctggctt
     7381 caattccaat ttgacccgac ggatcgcttt ttttacgcca tgagtccttg cataatttat
     7441 cccttttttc agtaatcttt tttttctttc ccatgaagat tcaatgccgt catcataagg
     7501 gtatttaccg tttaatttca cagctatgcc attttgatgt ctcgctgttt ggaagtctaa
     7561 gatggctttt cctctcattt tccctgataa acgtacctta aaaccgcttt gggtcttaac
     7621 atcaagatta tataaatgat tgatatccaa tcttacgaca tgtgttgctt cagcaatcac
     7681 attttctttt tctaccttta tcgtagggat ttctttggtc acttcatcaa ttgcccagcc
     7741 cacaataagc aaatcgccag tggcccgttc acgagtgatt tgatcgataa atacggcaat
     7801 ttcttttttt tgcacgattc gtttactcct tatctttata agttaatcat tatttacatt
     7861 tcaatgatcc atgattggcc attttgagct actgcttcca ctaagaatga acgttcttgc
     7921 tcgcaaagga ttcgtaactc aaatcccgct tctgttcctt ttgcgagctg ttctgcttga
     7981 ttgacttctt ctcgtgaaac aggaattgct tcgaaaaatg gcgattggtc agctgataac
     8041 ttataggaca agggctggcg atccgaatta gcgattcccc atccgatgat ttttaatatt
     8101 tttgtttctg catcccagat tttctcgtca atacatatag aaagttcttt ttcttggatt
     8161 tttttcgaag tttctttctt acgtcgtcct agtctatgtt ttattgtcca ttcatcactt
     8221 tccaacaatg tgcaatagcg atttgtggtt gtttttaacg tatggtctag tcttgtattt
     8281 tcctcaagaa gatgatgatt ttctctttct aattcgtcat ttttctcaga aagctgcttt
     8341 gtgacttcag ccatgggacg aatcgtttcc aacaattctt tcattgttgg tgtcaattcc
     8401 ccgataaagc gatagtgaca atggatagtc acttctttcc cagaaatatc cgttagaacg
     8461 aactctggta attctttccc atcaaacaaa taacaatcat ttactgtttt gactacagca
     8521 ttcgaatcga taaatgttag tttttctccg gcagcttctg cgctgaattc gataaagcta
     8581 ggttgttgcg cgaagcgaaa caccattctt tctgttgttt ctgatacggg gaaacttagt
     8641 gtctcatttt cccctgtgaa attattgaat ggtatttgtt ggattgtttc atcttgtttc
     8701 gtttgctggg ttacttctaa cacttttaca taattagaat tttgtggctc tgcaatggaa
     8761 ctttgtgcta ctggatgttt catcaatgag aaaaagtatt ggtagacttc accaaatggt
     8821 cgcattttga aatcatagcg tacagcttcc ggcaattctt cataagttga ttttaattct
     8881 gtatcaccta cagctaagta caaataatct tcaatattaa tgaataatcc cgctttttca
     8941 aaaacttttt ggaagccatc atgagtataa aaagaattat gggtttcatc taggagacca
     9001 taagaagccc acggcaattg gttattgaac aaatcgatca tgacagaatt atgcgccaag
     9061 ttagggaatg taatcaggat ctctccttct tcattcaaaa attctctgac tttcttcaat
     9121 gtttttccag gatctaccaa atgttctaaa acatccgcaa acaagacgta atcaaatgtc
     9181 tgtccagcat aatagtttgc ccactcaaaa ctttcgatat caccataaaa tccatcttga
     9241 gcaaattcgc tgacaaaatc gaacaattcc ttatctaact caacaatgct cacttcacat
     9301 tgttttgctc cgattaaatg tttcgttaat cggccgtttc cgggaccaaa ctctaaaact
     9361 ttacttcctg gtttgatctg tgcggcaatt tttcccacac tgctttgttc atctaggtcc
     9421 atttcaaaat catatttcat tgctatgagt cccttcttcc catttatggt ctaagaccac
     9481 gatgccttct ccgatataag aaccgctgac aaacaagttc atgtactttg tcttatatac
     9541 caaaggaacc gttgcatttt cttcaaagac cgcaacgtca aaataatatt caccagctaa
     9601 caaacctaac ttttcatact ccagataaaa gatattctct ccttttttcc aaggaatctt
     9661 gataccatct agtagagtat taagtccgca tacataaaaa ttgtcgactg ttcgtagcgc
     9721 aattcctaaa accggatttt tgattgtatc atcttttact tcataagtta ctttaacgta
     9781 tagtttttct ccttgttcca gcatatccac cggatttttg tctttatcca acaattccgc
     9841 tttcgtcacg tcgacgatat ctggagcggc gaaccgttct acttggtttt tattccgatc
     9901 cactgtttta actgattttt tctttaaaaa gttttcataa ttcgtcgtta cttccatcgt
     9961 atcgccatat tcgacgactt ctccgttttt caaccaatag gaacgatcac aaaaacgacg
    10021 aatcgaactg atatcatgag aaacgaataa aatcgtcttt cccgagttct tgatttctgt
    10081 aaatttttcc atacatttta gttgaaattc aagatcgcct actgccaatg cttcatccac
    10141 aatcaaaata tcgggattca cattgattgc tacagcaaaa gctagacgaa cgaacatccc
    10201 gcttgaataa gtttttacag gttgataaat atggtctccg atgtcagcaa attcaatgat
    10261 atcatcgacc atttcatcga cttcttctct tgaaaatcct agaaccattc catttaaata
    10321 gatattttca tagccagaat actcaggatt gaatcctgat cctagttcta gcaaagctga
    10381 gatttttcct tcgatttcca tcgtgccgga tgttggtgtc agtactcccg taatgatttt
    10441 caacattgta gattttcccg atccgttttc tcccacgaaa ccgatcatct cacctttttt
    10501 tatttctaca ttcacgtttt tcaacgcata aaatacatca tgataggatt tcttaaaagg
    10561 attgagggct tcacgaaatc gatccgaagg ttttgcgtac atattataag attttgtgat
    10621 atcttttagt ctaattgcat attctgtcat agctattctc cttacagcac atctgaaaaa
    10681 tgtggtttca atctacggaa cactgcagag ccgattacga acattactaa cgtgaaaacc
    10741 cagaaataca aagtatatgt tccatgctcc cagaaccaag cttcacttaa aaatgactca
    10801 cgataacctt gaacaacata gtacagcgga tttaatttca atatgcttac aagacctgaa
    10861 tcaaactgat tagggctcca taatatcggt aatgtccaca tgatcaactg cagcacgatc
    10921 gtaattaatt gcatgatatc aggtaaaaat ggttggatcg atgccgtcag ccaagtcaat
    10981 cctgtcaaga aagcacacat gcaaaataaa taataaatca gctgtaaaga cattgctgaa
    11041 ggatatatac catttataag cgcgacagct atgccaatca gaatgaagaa taagtgggtg
    11101 tacagattgg ataggatttt cgtcgtgggc aagatacgaa tattgaatac gactttcttt
    11161 actaaataag tatattcccg aaaaacaacc gtagttgatg caaatgcatc tgagaagaag
    11221 aaccagggaa tcatcccggt aactaaatat acgataaatg ggtattttgc atcgatcatt
    11281 tgtgctttca gtccaacacc aaataccagc caataaatca atacagtaaa tagcggattg
    11341 ataaacgccc ataagattcc taaaaacgat cctgcatatc ttgatttgaa gtcattgatc
    11401 gaaaactgta gcaataattt tctgttctga taaatgtcct tcataaataa atacaaatct
    11461 ttaaacattt gtgttcctcc aagcgatttt cagtgaatta cagtcaagtg ccattttacc
    11521 atatttttgg taccgagctg cgttcttatt tctttcttaa ttttctatga tacaagattt
    11581 tctctaacgt ttctatttta cagaagatgc tagacaattg caataaacca aaaaaggctg
    11641 cgacggttgt cacagcctct ctcgtatttt gtgcctatca atgatagcgt tggatcctgg
    11701 aagaatgagc gctatctggt tgttcctcat aagatgtatc atcataagtc atatcgtcat
    11761 atgcctcttc cataacatag aattcctccg caacttctgg ttcatcatat cgatgaacgg
    11821 ccatagccaa caggatataa ataatcggaa cccatgggta tgaagtcaat gtatcctcca
    11881 taaatgactg catcatcacc gcaaaaaagg cccagataaa agtcatctgc ccgttggata
    11941 aatctgcttt caacgttaag cgaccaataa aaaggataag cagcaaagca gcaatcccaa
    12001 atcctatcaa tccatagata ttcaaaatag aattataact attgtggttt cctaaaaatt
    12061 ggtttcctcg ttggaagata aatgttttca ttcctagtaa aatttgttca gatttttctc
    12121 ctagcgtctg atagaattta tgccaaatat cttctcgacc ggtaaacaaa ttgacttttt
    12181 cagagacagc cgccagataa gaaatcggca aagctaaaag aatcgtaacc gtgtaagcaa
    12241 taaatggcgc acgaatgaca cgcatcaacg atttaggtaa aagatccaat atcgcaaaaa
    12301 caatcaacgc gactaatgca cctctagatt ggcagaccca aatcccgaga aaagctgcaa
    12361 caactgctac tgcaccgatg attcgtacat accatcgttc aaaagaactg gcaaatccac
    12421 tgatcaataa agcaagagtc atcaaagaac ttccgattat attcgtattg atccaaatat
    12481 cagaaagcga attgcctgga gcccagatgc tttgtccttc tggtacttgg atctctgtaa
    12541 atacacgata taacgtgatt ccgaggaaga ccgaaaagac aatcagataa ataccgcgat
    12601 cccattttgt ccactcgatc catttaatta ctaaacaaaa taaaataatc aaaggaaaca
    12661 gtaagtctcc aaataaagac tcatttctta attcccgaaa gtaagaaaag aaaatgaaaa
    12721 gggcaagcgc aacacctaga taaagaaaat cgacgccttt tgctttataa acatttaaaa
    12781 tgagcaaaat agccacacta aaagcgataa gtaatgaact attgttatat ataaacaaag
    12841 ttgctggaaa acctaaccct gctgtccaaa caaaaaagac tagcgtcagt aacaaaagca
    12901 agaaaccgac actcattttg ttgacaaatt tttgtacgat tgcttgaatt atctccactc
    12961 tcctttctac taacatttca ggattattct cagctagcct attactgtac gactagctga
    13021 ttttcctgag tttgtgtttg tggtacatct ggataatccg gatattctac tttaaaaatc
    13081 atataaccgt ctttatccgg tccatatagc tgcgtgaact gcgcttcata taattggttg
    13141 taatcagtta atggtcgttt actgatcaca tagtcgatcc ctaatttttc tgcgtcttca
    13201 aaattcaacg tgaccgaaaa catatctgga cgatctaaaa tgaactttgt atcttcctta
    13261 gcgataaaag ctcttgtatg agcataccga ttataatact tctcgtattc ttctttcgga
    13321 tcgattttct tccatgcagc tatatctgga taaaagcgta ctgtattgaa ggatttcact
    13381 cccaatgttg gggtgaagtt atataattca tcttcagaca accaaacttt accaggatct
    13441 tgttgatcga ttttttggat ttcttttgcc agtgtttttt caaaaatcgc tcctgtacct
    13501 tctacgactg gattgacgaa aaagccagat acgataacta aggcggcaaa agcgaggtaa
    13561 aacaagcgtc tccagccaaa taatgcaaaa gctaaaatca ctgttcccaa cacagcaact
    13621 gcgatggttt caaaatccga aaaataatta tccattttcg aatgcgtaat cgcatagaaa
    13681 taagaccatg catttactgc caacaaaatg aatttatacc aaataggaat aacatcttct
    13741 ctccaaatat aagcgataaa ccagatcgat aataaacaag ccgcaaaact aaaagtaagg
    13801 atggctctta atggaggaac ataagacagc agagtgattt ctgctagcca ttttggcaac
    13861 ccaacggtaa tccaaaagat agaaaacaga caaaaaatca tcaaaatacg tcccagtaac
    13921 ttttgttcgc tatctttttt gccaaataac acaaatggac ttgctaggaa tacagttgga
    13981 aaaaagtgat agaacagggc cacttcagag ttattcgaat agttgatatc ttcaaaagga
    14041 attttccaat ttgtaaggaa agaaaaaaat tcgccaatcg tccactttcc tccagtactt
    14101 acccgttttc ctggatataa ggtattcaaa gaagctaaca atgcgtcttt tgacgttagc
    14161 cagaaatgaa ccatgactcc gccaataaat aaaactgccc ctccaatcaa cactacatcc
    14221 aacagatccc atgaaacttt cttgctatag taaataagca ggccaacaaa ataaaaaatc
    14281 agcaagtacg ccagcatcac ttgatgggct gggtaaatca ctaaaataaa cccaataccg
    14341 aaaataacca ccaaaagcat catcaacgtt cgtagccatt ttttatcatg attgtagaaa
    14401 taatgataaa aagcagtcat cagtcccaaa gtaaagaaaa tcaaatcgcc cacatgttgc
    14461 atgaaccacc actggactgc tggagaaaat gtcagcgcaa atgcaccgat tagcgataga
    14521 gctttttttc tcttcgttaa tatcatcagt acttcgaatc caagcaagac catgacgact
    14581 aatttcatcg cccaataaaa ggaaagtcct cgatctcttc cgaggaaaaa gaatccccag
    14641 ttgaatggtt ttccgataac cgtaatatcc tttacaggag aattatacgc aatgatcatg
    14701 ttttgtccgt ttaatgaata atcctcgttc accaacggaa agtcctcttc agcttgtgag
    14761 aggtaaaaag gtgtctgcac cagccattcg tctgaccgca cttcccgatt ctgtccaaaa
    14821 atcacatcag tttttttccc atcgtctctt tgcgacacat atttatccca aacaccaagt
    14881 gaacttccgt ttaaattaaa agtaacgatg accacgatga ctaaaacgcc aataagatag
    14941 cggagtttta gaaacaggtc aagcaccaaa ttcgaatctg cctggttcgc ttttggcttt
    15001 tcttttcgtc gtgcacttct tgtttgtttt tccatatcat gctccaaatt ctatttgata
    15061 aaaggaaatt acttattcat ttttttggca gcttccagca tttgcgcaag tgcttcttgc
    15121 caagttggta tgacaaatcc taactcttct gtctttttca ggctcatgac tgaatactgc
    15181 ggacgtgttg ctttttgcgg aaattcttct gacgtaacag gtgcaacttc tacatctgta
    15241 tctttcaata tttcttttgc gaattcatac caagaacagc tattttcgtt cgacaaatga
    15301 taaataccaa atggtgcttt ttcttcgatg acatatacca taaattctgc caaagtacgt
    15361 gtccaagttg gtcgcccaaa ctggtcattt actacagtca gtttgtcgcg ggtttccgct
    15421 aggcgttgca tcgtgaagac gaaattgtgt ccatagatcc cgaaaaccca agaagttcgt
    15481 atgatataat agtcttctaa aatctcttct actgcttttt cgcctaataa tttcgtacga
    15541 ccatattcat ttagtggatt cgtttggtcg tctacttcgt attctacatt tttgtttttt
    15601 ccatcgaaaa catagtctgt actgatatag acgaattgag cattcacagc ttttgctgca
    15661 agtgctacat ttcttgtacc atcaacattg atcttctcat ctaattcttt tgcttcttct
    15721 tctgctttat ctactgctgt gtaagctgca caatggaaaa taacatcagg ttttaattct
    15781 gtgatgtaag ccattgtttt ctcggcatct gtgatatcca agtcttttgc atctgttgaa
    15841 acatattgca gatttttttc atctaataaa tggcgcaatt ctgtacctag ttgcccattt
    15901 cctccagtta ataaaaacat atgattatcc gctccttgat ttttatttta aaaaagagac
    15961 aggcgagcgc ctgcctcaca tgtgtttttc cattaataca gtgacatttt ctctgtatta
    16021 ttgtccattt ttggcatatg ctgcttcaac ggcctctttt tcttttttcc accagtcttc
    16081 atgttcttcg taccatttga tcgtgtcagc caacccatca cggaagttcg taaactcagg
    16141 cttccatcct aactcttccc gtaattttgt cgaatcgatt gcataacgca agtcgtgtcc
    16201 tgcacggtcg tttacatggt cgtacgcatc ttttggttga cccatcattt ctaagatcaa
    16261 ttccatcacg gtcttattat cttcttcacc gtctgcaccg atcagatagg tttcgccaat
    16321 tctgcctttc gtcaaaatca gccacactgc tgaagaatga tcattcgtat ggatccagtc
    16381 acgaacattc tttccttcgc catataattt tggacgaata ccgcttaaga tattcgttac
    16441 ttggcgagga ataaattttt caatatgctg ataaggacca tagttgtttg aacagtttga
    16501 aatggttgca cgtaagccaa aagaacgcac ccatgctttt actaacagat ctgaaccagc
    16561 ttttgtagaa gaatatggac ttgaaggatt atatggtgtt tccgcagtga atttttctcc
    16621 ttcgccttcc ccatgtcctg gcaggtcttc acgtaatgga agatcgccgt aaacctcgtc
    16681 agtagatacg tgatgatagc gtacatcata cttgcggcaa gcttctaaaa gtgtatacgt
    16741 accaatgata ttcgtttgta caaatgggaa aggatctttt aatgaattat cgttatggga
    16801 ttctgccgcg taatgaacaa ctgcgtccgc ttcttttact agacggtcca ctaattctgc
    16861 atctgcaaca tctcctacga ccaattctac gcgatcgcta ggaagtccag ccaagttctc
    16921 tttgtttcct gcataagtca gtttatccaa aacagtgaca tgcacttctg gatgattgtt
    16981 taccacgtaa tgaacgaaat tagagccgat gaatccggca cctccggtaa caatgatgtt
    17041 tttcataaga aaatttctcc ttaattctta gatttctccg taaacaaatg gattttctgc
    17101 ttcaaattct tttaatgtcg gatgacggtg atctttttcc gacataatcg ctcttgatgg
    17161 atcaattggc caatcaatcg ctaaatctgg atcatcaaat gcgatgcccc catctgcttc
    17221 tgcattatag taattatcac acttataaag gaagttcaca tttggtgtca aagtaacgaa
    17281 tccatgggca tatcctttag gtacaagcaa ctgtcgatga ttatgttcag ataagataaa
    17341 tccgtcccat tgaccataag tcgggctacc tttgcggata tccactacta catctaaaac
    17401 tgctccagtg actacccgaa tcaattttgt ctgtgcagct tctccttttt ggaaatgaag
    17461 cccccgaagg acaccagctt cagctgatag tgaatggttg tcttgaacaa agtcatattt
    17521 caatccatgt tctgcaaatt ttgcttttga ataactttct gtgaagaaac cgcgatgatc
    17581 gccaaagacg tccatttcta cgattttgac atctgttaat tttgtatctg ttactttcat
    17641 ctgtaaggac tccttattca gcggccaaat tcaataaata ttgaccgtat tgattttttt
    17701 tcaatggctg tgcaagggcc agcaattgtt ccttatcgat gtagcccata cggtaagcaa
    17761 tttcttccaa acaagccact tttaagttct gacgtttttc aattgtttcg ataaaggtag
    17821 atgcttcaag caatgattca tgtgttcctg tatcaagcca agcaaatccg cgtcccataa
    17881 gttctacgga tagttttcct ttatccaaat atgctttatt gacatccgta atctctagtt
    17941 ctccacgttc tgaaggtttg atacctttag cgatagaaat tacctcattg tcataaaaat
    18001 aaaggccagt tacagcgtaa tttgattttg gctgagcagg cttttcttca atcgacaaag
    18061 cttgcatatt ctcatcaaat tctactacac caaagcgttc tggatcattg acatgatagc
    18121 caaatactgt cgcgcctgta tcttttgaag ctgctcgttg caacatttta gacagaccgc
    18181 caccatagta aatattgtcc cctaatacca agcagacact atcatctcca ataaattctt
    18241 ctccaatgat aaacgcttgt gccaatccat ctggactttc ttgtactgca tattcgatct
    18301 gcagtcctaa atcatttccg tttccaaata attccttgaa acgtggtgta tcttgtggcg
    18361 ttgaaataat cagaatttct ttgattcccg ccaacatcag agtagacatt ggataataaa
    18421 tcattggttt gtcataaatc ggcatcaatt gtttcgatgt tgcctttgtt aaagggtaca
    18481 gacgtgttcc gctgccccca gcaagaatga ttcccttcat ggatatctgc tctccttttt
    18541 gatttcaaat ttcactttgt tattatctca tcaattgtta tatttgtcat ggaaatatta
    18601 aggatttaaa catcttttat caacgtttaa accattttcg tttgatcaac gccgtaatca
    18661 gatgaacacg ccaagaaatt ttttgccgta atttagaatg tgtttcaatc tcactcgcgt
    18721 ttaaatcatg tcttcggtac agagttaacg gttctcttag gaacccagtc ttctttcctc
    18781 ccagcaaacc aatccacata tcatgcatcg gcacttctga cgggatcggc aaaatatcgg
    18841 gttttaaaga agaccggaaa gccataccag caccaatata gtgacttctt atcacattat
    18901 taagccatcc ttctttggaa tttcgaaacg aaaaatacga atcatggatc tcttgcagct
    18961 cgctgtccac aatgattaaa tcagaaacta ctactagctg atccggattc tgctcaaaat
    19021 agtcagtgat ctttttcact tttgtctcca accaaacatc atcttggtct gccaaaaaga
    19081 ttagatctcc tttcgtctgc tggatcgcat aaccaaaatt agcgatcaat ccttgacgag
    19141 gccccgaaaa aagctggatt cgcttatctt tttctgcgta ttcagttaat atatcccatg
    19201 tatcatctac tgacccatca tcagaaatga ctacttcatc ttctgctgaa agctgagata
    19261 aaatactatc taactgctct cttaaatatc tgcctccatt aaaggtagct atacaaaccg
    19321 aacgcacgaa ttcacacctt ccaattctta acatatgagc gcaaagtcat ccgccagata
    19381 tgccggtttt tgacggtcaa gaactgttta gctgttctta acaataattg tcgtcggtga
    19441 ctggataaat gcctttgatc atattcatga taaaatcgat tttctgcttg taaaatagaa
    19501 cggtatcgat tacttgaaac tttttcatag tctaaaaccg aaagatcatg ttccattcgt
    19561 acaggaagga tctccacagt tttttccaac tgatccactt tccaaaacag ccaatgatcc
    19621 aaaaaatcta aagaaaaagc aggattaaac ccgccaatct tttttagaaa tttgacagac
    19681 caggcaacac ctgaattgat tgccatgatt cgttcagctg ttgggccttc aacttctact
    19741 gcttgagctg tacgattgat ataacgatct gaatataggg gagaaacttg tcggctccca
    19801 gaaaaaatca tcggaactgc agcaacacat tcatctgtaa aggttacttt acgcaatagt
    19861 tcaaaataat cgtcgactag cctcgtatcc tgatccagag tgacgagaca acgtgttcct
    19921 tcatctgctt gagacaacgc aaaattataa gccaccgcta aaccaggatt ttgcggatca
    19981 tgatgataaa cgatattttc tttttcaaac agctcatttt gctgcctttg aggactatta
    20041 tcgtaaataa taagctgaat ttgcttttcg ctaagtgctt tgcttaatgg tccataagct
    20101 ggagattgac tgaatagttg ctgataaagc acaacgacta ctaatatttc tggtttcatc
    20161 agaaaaaacg ccacccccac ttattgaaaa atttcatcat agaagtcaaa aacacgcgaa
    20221 acagttttgc atttcgatgc gcacccttcc cgtaaaaatg gataactgca taaaagggtg
    20281 tgtacaataa cttgtaacct gctttttcaa aactcaaaca caagtcgttg tcttcaaagt
    20341 acatgaagaa acgctcatca aaccccttta tctctttaaa tttatcaata tcagctacca
    20401 taaaactacc tgagatcatc cgtacataac tattagcatc agtaggcaat cctcggcatt
    20461 caaacacagc cagtcgtttg tcaaactttt ttttgaccca tttgaaaggc acaaagcgca
    20521 aaacgtaatc aaaaacagcc agctgctcac ggatgaggtg ctgagttgtt ccatcttcat
    20581 taagaatctt aggtgctaac atcgcacatt ccggatgttc tttcaataac aagaccaatg
    20641 ctgtaactgt ctcttcatcc actaaaatat ctgggttaca aataatgcca tagcgagaat
    20701 cgctagctat cagattgtga ttatgtccat aaccaaatcc ggaattttca tcatgaaaat
    20761 agatatcgat gtagggatat ttcctatact ctgttagtct ttttttgtac tcgggaagtg
    20821 aattgttgtc aaaaattcgg atttttactg ctgtttcatc ttttacagtt gaaataaaat
    20881 tatctagtgt ccgaaaaatt ttatcactgt ttgccgtcac aatactaata aggatcggct
    20941 ggtttaattt gttattcatc tatatgacgg ttccatttct tcaaatattt ctgttttact
    21001 tgcgtacggt actctttatt ccctaaaaat cgcaatgttt ttaaaataaa ctgatgtttc
    21061 tcattgatca gaccaatcaa ttcaaataat aaaattgcag caaacaacat ggcaataatc
    21121 aatatgatcg ttccgattct tccagtataa ttcatgacca tagctgtgaa agaaaacatc
    21181 aaagccatcg cataaatcgt caggacagca cctttatgag taaagcctaa actgattagt
    21241 cggtgatgaa gatgtttttt atctgctgta gaaaaagaaa cacggtttgc ttttctccta
    21301 atgatagcaa aaaacgtgtc tgttaccggc actcccatga cgatcagcaa agaaatcgat
    21361 gaaacaaaag tcacgttctt cagcccttgt agtgaaagga cggccatcat aaaccctagg
    21421 aatagagctc ctgtatcgcc taaataaatc tttgccggat aaaaattgta aggaaaaaaa
    21481 ccgataatac ttgctacaag acagaaaata acaactggaa tatacactgt agaagcatgg
    21541 aggaaaaaat atccaatgat tcctatcgta attaaaccga tcaatgaaat ccctgccgcc
    21601 agcccatcca aaccgtcaat cagattgacc gcattcgtga taccgaagat ccaaaaaatc
    21661 gttacgggaa aactaagcca gcctagttgg atatatccaa taaaaggaag cgtgaaggaa
    21721 tcaatacgta tatctgctac aaaataaata ataagagaag caatcaagat ccctatactt
    21781 ttttgtttag gtgtcagctc atatttatca tcgagcactc ctgtgactat gattactaaa
    21841 gaagcaatca agatcggtac aatatacgat tgagggataa ttttacggaa taagaataaa
    21901 gtcgatataa caaatgaaat aaagataggc agtccgcccg ctgaaggcat tggaacttta
    21961 tttattcttc tttcatttgg tttgtcgacc atacctgttt gtactgaaat gattttgaat
    22021 acaggtgtta atatcagtga taataaaatt gtcagaaaaa atcttataac catactaaaa
    22081 gaaacaagca tatgggcacc tactttaatt aagagtcata attattataa attatatatg
    22141 tctttttgac tatcttttta tatcttctca aatgtccgca aagccttatt ttatcggctc
    22201 tacgggcatt ttgcttttgt ggtaaacctc gcatatctag gtctatcttc ttatattttc
    22261 cctgtcaatc gtggttaaaa tcgtggtaaa tacttctatg cgattagacg ctgaacctct
    22321 gattttgcgg tatctatgga cgcatgagcg taccagttca ttgtgatact gatgtttgaa
    22381 tgccccatga tatactgtaa atctttcggg ttcatgttct tgcttgccag ccttgtgcag
    22441 aatgtatggc gtagcgtatg cggtgtaata tgtggcaagg gattgtcctt gtggtgcttg
    22501 ttgtatttct ttaccatacg gataaataag gcgttataat caatggcaac ttttggatta
    22561 cctttcggat tgataaacac aaaaccgctg tgtccgtcta cctctatcga ctttatcttt
    22621 ggtcggctgt tgataacccg ttgaaatgcc tgtatcgttt ctttgcttaa tggcacttgc
    22681 cttatcccgc ttttcgtctt aggcgtttca atataatagc cctgttcctt actctttagt
    22741 aactggtggt cgataatcac aacttcattc ttgaaatcaa tgtcggctac tgtcagtccg
    22801 cacagttccg agatacgaag tcctgtcttt aacagtatca gcacatcatc ataatgcttg
    22861 tgatacacgt tgtccgtctt aataaatgac aataaggctt gctcctgttc ctctgtcaat
    22921 gcgactttct ctttggtatc attttctatg acttcactta gcttgaaatc aaaagggttt
    22981 ttcctcacat aatcatcttg tatggctatg tagaatgacg cttttaacga gcgtttatgg
    23041 ttgttaatgg tattgtagga aaagcctttg tctttcatgc gtaacgccca ttctttagcg
    23101 tcagagggtt ttatcgtatc aatgctccta gcacctaact tgtcctcttt taataaccgc
    23161 atgagttgtt cccgttgttt ctgtgtgctt ttcttcacat ttgtcctctg tgcgttctgt
    23221 ttggcataga gttggcaaag cgtcattttc ttgcctgtgc tgtcgatacc gtcctcaata
    23281 tcccgtctta tctgctgttc cagttctcga agtgagggtt tttcccgttt tccctttggt
    23341 gtcgggtctg tgggtgtcaa tctccaagca tacacatatt ttgtgtttcc caatgcgtct
    23401 acatatttat ataagtattt tccgtctgtt cgttggctct ctccagtatg caggatacga
    23461 cctttgttat cccgtctttt ttctttcatg gtgtctgctc ctttccttgt tggaaagagc
    23521 cttgatatga cttgtgttca tcataacaca tacaaggctc atttgcatta gattgcgtcc
    23581 aatttatcaa taaccttttc aaactgtcgg cgtttaatct gtatgcggtt gccattcata
    23641 atgagccagc cagcgtcctt gttttcctct gccaatcgtc ttaacttgtt ttctccaatg
    23701 cggaaatact ttgacgcttc ttcaatggta agggtgtatt tttcccatac gggaatatcc
    23761 tgtgtattac tcataaaaca ccccctcact ttcgattacg tctgatatgt ccgtacacat
    23821 ctgttccaca agcatttcct gtttttcaga aaggtgggta cattcatcaa tcattttcat
    23881 gtagtttgaa cctgtcagcc tgtcgatttc ctctatcatc acgattgacg gcatgacctg
    23941 tctttccagc cattgcagag tgcgttccat attgacaggt acagggttca tttcaaaagg
    24001 taaactgtca tgggaaatga atatctccca caatggatag tcaggaaatt ccacaaaatc
    24061 ggtaatgaga taaaacacca gcccatgagg gttatacgtc aataacagtt cttcaactgc
    24121 ctgtaccgcc tttttgtcac gcagacgaat ttcaaaacgg ttgataatat cggtatgttt
    24181 ctttttcgtt gcctgttcct tttgtttttc atacaggcag aaatattttg tgcttgcctt
    24241 tgagccgata taaagggtac ttgctaaatt acggtttttt ccgcttaatt ttccaccttg
    24301 tacattttca tagtttttac agcgacattc cgcaccacca tttctgtatt tttcagatag
    24361 cacaggaata tccagcagac cgcacatatc attgattgcc aagtcaaaac gtcggataac
    24421 accgccacag tcaagacagc ggtttaagaa tgaataccag tccctgttct gtgcctgtaa
    24481 gacatattcc atgttgcgtg agcctgcccc ttttaattct aaaaatacgc ccatgtgttc
    24541 atcatcagaa gccatgacct tgatttcccc gtaagcataa tgctccttat agccataata
    24601 cccatagtca taatggataa aataatctgc tttcatgcca agcacctttc ggataacttc
    24661 caatgcgtca gtcgttggaa aacggacaga cagataatca acgagcagat aaaaaggctt
    24721 atcgcagata tagtaattca gacaacgcag gataatgttc tgtgtttcct tgctggcgtt
    24781 agcttttccg ttctcaaagc ggttgatact atatcttgat acatgggtaa gacttgcaag
    24841 ttctccttgt gaaagaccgc tggcaagacg tttgttcttc atttcaagtg caaattctaa
    24901 attgttcatt gtattcctcc aatcccacaa tagccttgaa atacctgtaa accacctttc
    24961 aaaaagtgtg atatgtacta tcttcttaaa acctctcatt actactcaca ggtttggggg
    25021 tgtatggttt ttgacttgtc ttttgtgaaa tagtgccccc ctgttagata acgggggcac
    25081 agacaaggct cgcaggctcg ccagccgaca aagcatttct tccccttgac acaaggtgtc
    25141 atggcggtaa gcctgagagg cttccgccac cgtgtcagaa atactttgtc agcaagttct
    25201 gttttgcctt ttctaacaag ttccatttca accgatattc atatctacgg atttaatcag
    25261 ataggtattt ctttttgaat gtttttatat ttcattctat tgtgcttatt cgattacaag
    25321 aacatagtaa ttcttatatg cttatttgtc aatatctttt tttgccttta caaatcatat
    25381 taagcatgat agacttataa tagcgaagat ataacgagat atggagggtt atgcaatgaa
    25441 aataagccat ttaggaaata atatacagac cataagaaaa tttaggggaa tgaaacaaca
    25501 ggaacttgcg gacaaaatcg gtatcaatat gcagagcctt tccaagattg aaagagggtt
    25561 gaactatcct gcctatgaaa cgctggaaaa gataatggaa gtgctggacg ttacgcccaa
    25621 tgaattgttg tcgggagaat ggaagtatgt caatcaatct gaaaaggaag tctgtcagtt
    25681 tttaagagcc gaagaacgtc taaatgcaga actgaaacat ggacactatg ataacttctt
    25741 tgacagcgag gaagaatggc tggaatatga gttagaaaag ttacgggaat atattacaga
    25801 ctatatcaac gggaaaagca ttgaagcgtc cgacctttac ccaatcaaag aatttatcca
    25861 gcatttgaag tttcaaaaat tattagaccg ctatgatgat ttatacagta tggatatgtt
    25921 tggggaaagc atagagggac ataagtataa gacaccttat caagtcgtga aaatgataaa
    25981 cccgaactca aaagaagata tggaactgtt acgagaagta ttaaggaata acaattttga
    26041 tgatgaggac gagtaacttt tttcctcttg cgagaagata cagcaaacaa aaagcccgat
    26101 caagagtgca aagcaccacc aatggtggca aagctcttga cagggctttt tcttttctgt
    26161 tgtgggcaat caagaggaag aaagggataa aatctgattt gtggtaaatc aggcaaaggc
    26221 aacgcaggta tcacagtatg aattgacacc gcataaggaa agcaactgga agattgtaga
    26281 ataaatgaaa aaggcttgca ttctgcattt ttataaaatt gcaagtcttt atattgacaa
    26341 gcataaaata ctaagatata ctttaatcag accaacggtt tgtattggag gtaagttatg
    26401 gcacgaaata aatatccaga agaaacagta aaactcattt tagatgtttc tacaaaaatg
    26461 ttttgtgaaa aaggatatga taacacatca ttgcaagata ttatcaatga aacaaaactt
    26521 tctaaaggtg ctatttatca tcatttcgat tcaaaggaag atattttaaa agcaattttt
    26581 caaagaattg gaaatgaaaa tgcagtaata tttgctaaaa ttagagataa cgatagttta
    26641 aacggtttag aaaagttaag agaaatattt aagacagcgg tattcaattc taaccaaagc
    26701 gttttattga cagtttctcc tcaactatta aacaatcctc gttttcttgc tatgcaaata
    26761 gaacaaatgt atgaaattgt agtacctgaa tttgttgaac ccgttttaga acaaggtatc
    26821 aaggataagt ctttggagat tgaaaattca catgaaattg cagaagttat catggtatta
    26881 agtaatgtat ggctcaaccc attagttgaa atgacggata aagagggtat ggaaaaacgt
    26941 tgcaatacat tcaatgccct tttaaatgga ataggcatag acacattatt agacgaagaa
    27001 acaatttctg catttataca gttttgtaaa taactgtatt tgttatccct atacgataga
    27061 ctgctctata aaagagcagt ttatttaaac cattatataa accgacggtc tgtttgatta
    27121 aataaaaagg agagatttta tgcaagcaga aaaaaaactt tttaatcgtg attttatact
    27181 tgttgttata ggacagataa tttctttatt tggaaacgct atcctacgat ttgcattacc
    27241 gtactatttg ttaaaagaaa ctggttcatc agctttgttt ggtattgcta cagcctgctc
    27301 ttttttacct atggttattt tgtcgctggt aggtggtatt attgctgaca ggacaaataa
    27361 aagaaacatt atggtcatac tagattttgt aacagcaatc ataattttag tattttattt
    27421 gtcattggga aaaattccta tcataccttt gtttattgtt gctttgatgt tactttatgg
    27481 tatttctggt gcatatcaac cagcggtaca agcaagtatt cctctattgg tatcatcaga
    27541 taaacttacc gtcggtaatg ctataataaa tcaagtaaat actttagcaa actttatcgg
    27601 accagctata agcggagtag tttttcaatt ttatggtatc aatcccattt taattgttag
    27661 tattatatgt tttacttttt cagccattat ggaaattttt atccatattc cacaccaaaa
    27721 aagacctcgt gaagtaggtg taattgcagt tgctaaaaac gatttaaaag atagttatac
    27781 ctttttgaaa aaggagaaac ccatttttat atccgtaatc tttttggttt gcattttcaa
    27841 tttggtttta agtgctgttt taattgttgg tctgcctatc ttaatttctg aagttttaaa
    27901 aatgccagat acactatatg gtttttcaca atcagcatta tcattaggtg gtttatgtgg
    27961 tggtatttta acagctcttg ttgcttcaaa gttaaaacta aaaaaccttt ataccttgtt
    28021 acttatttgt tctgtttctg ttttacttat gggaatttcg ctatttttag aattgcctgc
    28081 aatagtttcc tactgggtag ttacagtgat gtgctttgtg gctatgggtg tatctactat
    28141 gtttgttgtt caaatttaca ctcttgttca gacacaaaca ccaccgcaat tagtaggaaa
    28201 gataatggct acgctggtag cagttgctat gtgcggtcaa cctattggac aaatggttta
    28261 cggtatttta ttcgatgtat ttatacaaga aacatgggtt gttatgtttg tagcttctat
    28321 tgcttcaatc atgatttcaa tatattcaaa aagaattttt caaaagttag agatataata
    28381 caaggggtta aatagtatgt taggctaaaa acaatatata aaagaaaatt tgaaatctgc
    28441 taaaaggata gtgttataaa agaaagagta atattgcagt atttaaggct cgcacaaata
    28501 tatgttttgt gtgggctttt ttcttaccat ttttctgtat ttctaaaggg tataaacggt
    28561 attccgttta tatatttcag cattagcaga aagggggtga gattatgagg acatcttctt
    28621 tcgagcatat cgtcagatta cagttcaatt ctttaatgct gattgtcatt aagaacaaag
    28681 tgaaaagtcg aaacagacag cttgcaagac attctaaacg tgaagtattg ttttgtgaaa
    28741 tagccgaaac gaaacaagtt gaacgtggaa cgattgatac ctgtttttat gattgtgtat
    28801 catttaaagt cttgcacttt acaatttatg tatctgatga aacacttggt acagttttac
    28861 atagactgtc agaaaaacag cgtagtgcga ttttattacg ttattttcaa ggaatgaacg
    28921 acagagaaat ttcagaactg taccatgtat cacgttctgc tatttgcagt agaagaacta
    28981 gaggactaag gaagttaaaa atgcttttga gcgaaaggaa gtaaagaaat gagtaaagat
    29041 tatggctatc cctcttttga attgatttgc aaagcgtcaa gcggagatga aatggcagtt
    29101 aaagagattt tgaagtttta tgacggttat atttctaaat tatgcttgcg tcctttttac
    29161 cactctgaaa gtggtaaaat cattatgcag gttgatgaag aactaaaagg agaaattcat
    29221 acagacatga tgaaagcaat attgaaattt gaaatcggag tgaagtaatc aactgtataa
    29281 aaaaatcggg agatacacag tttcgtgcta tctcccttag tagttttacg gtcatatcaa
    29341 ggctcattca atcgtggtaa attcgtggta agatgagtgt ttttctatac ttcaaaatgc
    29401 ttgaatgtat agtgtttatg cggataat
//
LOCUS       DANKHL010000007        28666 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000007 DANKHL010000000
VERSION     DANKHL010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.009"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            239..946
                     /locus_tag="PW101_000224"
     CDS             239..946
                     /locus_tag="PW101_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDL2512657.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAEN
                     WADLFWEDTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKE
                     LKDQLPSIPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMV
                     RNKIRVICNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMP
                     STATMTIIKQMELLEDE"
     gene            1125..1466
                     /locus_tag="PW101_000225"
     CDS             1125..1466
                     /locus_tag="PW101_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512658.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            1459..2205
                     /locus_tag="PW101_000226"
     CDS             1459..2205
                     /locus_tag="PW101_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDL2512659.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            2211..2897
                     /locus_tag="PW101_000227"
     CDS             2211..2897
                     /locus_tag="PW101_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512660.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHNIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            2904..3737
                     /locus_tag="PW101_000228"
     CDS             2904..3737
                     /locus_tag="PW101_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HDL2512661.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEY
                     VINWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLP
                     I"
     gene            3754..4605
                     /locus_tag="PW101_000229"
     CDS             3754..4605
                     /locus_tag="PW101_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2512662.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            4602..4961
                     /locus_tag="PW101_000230"
     CDS             4602..4961
                     /locus_tag="PW101_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDL2512663.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            4976..5137
                     /locus_tag="PW101_000231"
     CDS             4976..5137
                     /locus_tag="PW101_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2512664.1"
                     /translation="MTTEEVIQMRIRSIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            5134..5439
                     /locus_tag="PW101_000232"
     CDS             5134..5439
                     /locus_tag="PW101_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512665.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKTR
                     RRMPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKNMDIDSKGRQFRYVEEK"
     gene            5439..5756
                     /locus_tag="PW101_000233"
     CDS             5439..5756
                     /locus_tag="PW101_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HDL2512666.1"
                     /translation="MNLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILDEYME
                     GSK"
     gene            5753..6139
                     /locus_tag="PW101_000234"
     CDS             5753..6139
                     /locus_tag="PW101_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512667.1"
                     /translation="MIPRFRSWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVEIFEGDVVLVNVSNGFDHLVNEKTVVQESEFHSGLVCKSLISGMEYRI
                     FSHQKMGYEYEVTGNIHENSELLEVE"
     gene            6136..6471
                     /locus_tag="PW101_000235"
     CDS             6136..6471
                     /locus_tag="PW101_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512668.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDIHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEQ"
     gene            6617..7186
                     /locus_tag="PW101_000236"
     CDS             6617..7186
                     /locus_tag="PW101_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HDL2512669.1"
                     /translation="MNKQEAIKKLESIKVIGNDALAACYNESINAGITLMKKIDEPKK
                     LVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLYSVIIAGEYLVKEISCSNEVKLVSTAYLPQYLAH
                     HYQLTEKQIKAIDERYWPFAVPVEEVAEG"
     gene            7183..7356
                     /locus_tag="PW101_000237"
     CDS             7183..7356
                     /locus_tag="PW101_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512670.1"
                     /translation="MSRQIMLHIPDGTKAMTITIVAAQKDGGLALKTKSIDTKQILEG
                     RDVEIEIDEEEAE"
     gene            7353..7658
                     /locus_tag="PW101_000238"
     CDS             7353..7658
                     /locus_tag="PW101_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512671.1"
                     /translation="MNVQVYLKRDLARFPRSWDTNSHPSEAEEEGYLMSANMIEITRD
                     DADEFDKKRSWYSVPNPMYTAVIEDYHISDRFILIDLERPKKRIKQYRRCGFSTKKW"
     gene            7682..7879
                     /locus_tag="PW101_000239"
     CDS             7682..7879
                     /locus_tag="PW101_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HDL2512672.1"
                     /translation="MNNRHRRVAKLRNQELNVAKVKFEREYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILDIKWEDTE"
     gene            7876..8082
                     /locus_tag="PW101_000240"
     CDS             7876..8082
                     /locus_tag="PW101_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512673.1"
                     /translation="MKIYVVKFGNQFYRSDERSIGANTLSIVDILQSARWFDNLEEAN
                     QVSRRLGGLTQVYELVTVDHEGVE"
     gene            8082..8378
                     /locus_tag="PW101_000241"
     CDS             8082..8378
                     /locus_tag="PW101_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512674.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFRGYVEKVLSNSAIIRIENTMECDKWLAKSKENLAVARLVDMEVIESK"
     gene            8454..8867
                     /locus_tag="PW101_000242"
     CDS             8454..8867
                     /locus_tag="PW101_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDL2512675.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            9551..9718
                     /locus_tag="PW101_000243"
     CDS             9551..9718
                     /locus_tag="PW101_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512676.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            9744..10088
                     /locus_tag="PW101_000244"
     CDS             9744..10088
                     /locus_tag="PW101_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2512677.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            10093..10374
                     /locus_tag="PW101_000245"
     CDS             10093..10374
                     /locus_tag="PW101_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512678.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVDIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            10477..10791
                     /locus_tag="PW101_000246"
     CDS             10477..10791
                     /locus_tag="PW101_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDL2512679.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            10769..12463
                     /locus_tag="PW101_000247"
     CDS             10769..12463
                     /locus_tag="PW101_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2512680.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            12483..13661
                     /locus_tag="PW101_000248"
     CDS             12483..13661
                     /locus_tag="PW101_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2512681.1"
                     /translation="MGIFQKAVGYFTKKATVTLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            13624..14310
                     /locus_tag="PW101_000249"
     CDS             13624..14310
                     /locus_tag="PW101_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDL2512682.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            14310..15470
                     /locus_tag="PW101_000250"
     CDS             14310..15470
                     /locus_tag="PW101_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2512683.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            15480..16355
                     /locus_tag="PW101_000251"
     CDS             15480..16355
                     /locus_tag="PW101_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512684.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIKIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            16352..16663
                     /locus_tag="PW101_000252"
     CDS             16352..16663
                     /locus_tag="PW101_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512685.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            16653..17006
                     /locus_tag="PW101_000253"
     CDS             16653..17006
                     /locus_tag="PW101_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL2512686.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            16996..17397
                     /locus_tag="PW101_000254"
     CDS             16996..17397
                     /locus_tag="PW101_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512687.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            17390..17794
                     /locus_tag="PW101_000255"
     CDS             17390..17794
                     /locus_tag="PW101_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512688.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            17806..18411
                     /locus_tag="PW101_000256"
     CDS             17806..18411
                     /locus_tag="PW101_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2512689.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            18431..18793
                     /locus_tag="PW101_000257"
                     /pseudo
     CDS             18431..18793
                     /locus_tag="PW101_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18796..18978
                     /locus_tag="PW101_000258"
     CDS             18796..18978
                     /locus_tag="PW101_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512690.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            18996..22415
                     /locus_tag="PW101_000259"
     CDS             18996..22415
                     /locus_tag="PW101_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL2512691.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            22466..23203
                     /locus_tag="PW101_000260"
     CDS             22466..23203
                     /locus_tag="PW101_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2512692.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            23213..25504
                     /locus_tag="PW101_000261"
     CDS             23213..25504
                     /locus_tag="PW101_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2512693.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNKPFLSFGAPNFR
                     YSNASYITDGDGSSLSINGSAGSSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKNFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDVRLKEDTAYDSTVLDQKRF"
     gene            25528..27609
                     /locus_tag="PW101_000262"
     CDS             25528..27609
                     /locus_tag="PW101_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512694.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSITSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFLAEIKGRMDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSIDFGGYDYSGNPNLMSKLKSSDFN
                     VGYHGSLTNVNGMLHFMSDGTGDIDMFTRINTPQLASGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYISVSFSN
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLGKNIANKGTTFPIKSSAY
                     EIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGDLKPVEGLTDVWSLTF
                     TPTKLEPGLPKDLRIFQSPKETAGACRIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNN
                     PKDYVWDLEPSYVENNLATDIKVAEITGKANNYTDGKVSEINSQLTASINEVDTTAKD
                     AQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALN
                     GKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYG
                     SDYTFAITYTI"
     gene            27626..27775
                     /locus_tag="PW101_000263"
     CDS             27626..27775
                     /locus_tag="PW101_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512695.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            27772..28218
                     /locus_tag="PW101_000264"
     CDS             27772..28218
                     /locus_tag="PW101_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512696.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            28220..28357
                     /locus_tag="PW101_000265"
     CDS             28220..28357
                     /locus_tag="PW101_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL2512697.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            28395..>28666
                     /locus_tag="PW101_000266"
     CDS             28395..>28666
                     /locus_tag="PW101_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512698.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT        10345 a         4279 c         6125 g         7917 t
ORIGIN      
        1 gatagaaata aacatattag tccagtttta aaaaaagaga agtaatctat gtgggcaaat
       61 tctgttaata tgcttttttg atttaacaca atcaaactac tgttggacaa aaatgacagt
      121 aaggaaagag ttgctccgat tactaaataa aaaatagttg ttaacaatat tatattatca
      181 atttttctgc tcatgttttc accccaccag aattatatca aaaaggagag aagaaataat
      241 gcaagaatta gtaattttga aaaataaaga agctgtgact acgagcttac aagttgcaga
      301 aagcttcgat aaaaagcaca gacatgtttt gacggctatt gatgaactaa aagagggggt
      361 tgccgaaaat tgggcagacc tattttggga agatacttat gttcatcctc aaaataaaca
      421 atcataccga attatttata tgaatagaga cggcttctct ctattagcaa tgggcttcac
      481 tggtaagaaa gctttaagtt ttaaacttca atatattgag gccttcaaca agatggaaaa
      541 agaattaaaa gaccaattgc catcaattcc agcaactaaa cgagaaatgg cattacttgc
      601 tctagctgca aacgaagaaa cgaatgaaag agtagacgct atcgagtctg atttaaacga
      661 tttgaagaac aatcaacttc tagctgaacc agattaccga acaatttcta atatggttcg
      721 caacaaaatt agagttattt gtaatcagca gcatttaaat agcaaagcaa aagcagaatt
      781 gtttaaagac ttgaatggcg gaattaagcg aatcactgga gcagtagcta gaaatcgtat
      841 caaggcaaaa cagttcgatg atgttatcga ttttattaac aattggatgc cttctacagc
      901 gacgatgaca attattaagc aaatggagtt gttagaggat gaatagagct gaagcgctaa
      961 aaataggcgt aataattgct aatcgctggt ggagacacaa taaaccaaac atcctaagcc
     1021 aacaacatat tgataagcaa aaagcatggc aacaaataaa aaagtgactc agccgaccaa
     1081 agcaatgagt cacaaagaaa atacatctaa ggagatgtta ccacatgaaa aaagaacttt
     1141 ccactctaga tcaatatttg actgatccta gttggggcaa atcgaatatc aaggaaacaa
     1201 gcaatcgaaa aatcagacgt aatcttttga cggatgaaga actagcatgt gatcaagacg
     1261 atttgggaaa ttttgtgagt atttgggatc atgtctatct tatccattta tcgaggaagt
     1321 ccaaaaaaac tgaatatatc tatgtcatcg aagatggctt gattgatgcg ctagaagagt
     1381 atgacagaga taacttgatt gatatctctt attacggatc aggtaagaaa tacattgctg
     1441 aaatggaggc agaatttgat gagtgaaaag gaacaacctt tgaaaaatag aagtgataat
     1501 acgcttttta ataccttata caaaataaat gtgaaagatg ttactgaaaa acgaaataac
     1561 ctcacttatc tttcgtgggc atgggcttgg gcagaagtta gcaaagtgtg tgaggcagta
     1621 gattatgaaa tctatcatga tccagaaaca tatctgccgt atgtctttga taagaaaaca
     1681 ggctatatgg tttttaccag tatcacagtc aacggagtaa agcgtgacat gtggttacca
     1741 gtcatggatg gtgcaaacaa ggcaatgaaa gatgagccat atacctacga agtcaatgat
     1801 tatcagtgga ataacgaaac gaagaaaaaa gagattgttg gaaaaatcga aaagcgagtt
     1861 gaagcagcaa ctatgtttga tatcaacaaa acgatcatgc gctgtcttgt aaaaaatcta
     1921 gcaatgtttg ggctagggct atatatattt gctggcgaag atatgccaga agatgtctcg
     1981 atgcttgaac cagctactca aagaagcaaa aagctattct tggatgcttt acaactggtt
     2041 gctaacaagt acgaaaaatc aattgatgaa gcaattgttg cattgactga tgcggcttct
     2101 ataaccgctg atgacagtaa atggaccaag agagacttgg gcattctaaa acgaggcgtt
     2161 aactggattg aagatcagta cagagaagaa acaaaagaga agtgatatga gtgtttaaac
     2221 cattgatcga ttcatattca gcggttctga aaaagttcaa aggaaaagac ataggtgcaa
     2281 cgatcaatga agaagtgaac attgatcgac taaagacgat gtatgacgga tatgatggcg
     2341 atcggattat tgaagttcga tttattgatc cacgtcgatt tactgtgcag caacgaaact
     2401 tcatctatgc gctgataggc gatattttca tcgatacagg catgccaacg gacttctgga
     2461 aggaattctt ttacttccgt tttgaaggcg tcacagggcg caaaataagc ctcaaagacg
     2521 aatcgaatac aactgtgagt gatgctaatg tcttagcaaa tatcatctta gatttcatct
     2581 ttgaacatca tattcctttc aaagaaggct atgagatttt accagcgaat caagaatatt
     2641 acttctacaa atgcatcaca aaaagagtct gctgcatctg tggcaaaaca ggagctgaca
     2701 tcgatcactt tgacaaagcg ctaggaagac gaaagcgcaa agaagttgat cattcagagt
     2761 acacatttgc agcactctgc aggattcatc acacggagaa acacaatata ggtgtgatca
     2821 atttcaaaaa taaatatcaa atcaaaggga tcaagttaaa ccaggaaaca attaagaaac
     2881 ttagaatagg agggtaaaaa atattgtctg acaacaaacg ctactactat ttaaaactaa
     2941 aagagaattt tttcgatagt gacgagatgg ttctcttaga aagtatgcca gatggctata
     3001 tttattctaa tattcttctc aaactttatt taagaagtct aaaacacgaa ggtaagttga
     3061 tgtttaatga caggattcca tttaactcta caatgctcgc aactattaca agacattctg
     3121 taggagtcgt agaaaaagcg gtacaaattt tccgtgattt gcagcttatt gacgtattag
     3181 ataacggagc aatttatatg tctgatatac aaagtttcat tggaaaatcc tcaactgaag
     3241 cagatagaaa aagagaatac agaaagaaaa tagaagaggc aaaacggaat ttaataactg
     3301 gaggacaagt gtcggacaaa tgtccagaca aaactacacc agagttagag atagagatag
     3361 agaaagatat agatatagat aaagaagaaa agaaaggtaa gtattctgac gaacacttac
     3421 gccttgctaa aaaattgcaa agtaatttaa ctgaagattt tccaaaagaa atgaacaaag
     3481 tagatatcga aaaatgggca gacacaatca ggttgatgga agaaagagat aaagcgtcta
     3541 tagaagcgat tgagtatgtg atcaattggc tacctacaaa tgaattttgg tttggaaata
     3601 ttagaagtgc taagaaattg agagaaaaat ttgagaagct caaattcgaa atcaaagcag
     3661 acaagaataa tcataaaaag caaagtcaaa aactacagta cagcaatcct agtgaatatg
     3721 acgacttgcc aatttaaaaa ggagatgcat cacatggaaa gcctagcaaa tgctatggag
     3781 aaactaataa gaagagtatt agtgcaaagc gggaaatgtc cagaatgtag cgaacctttg
     3841 tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac atgcatgagt
     3901 tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat atataacgac
     3961 agcttaaaag cacgagcgtt gagttttttt caaaatggtt cggtattaac agataaaact
     4021 ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa aattgcttta
     4081 gaaagagcta aaagctttgt aaatgatgtc ttactgaacc atcctgcaca tttcattcta
     4141 tcagggaaat caggaagcgg aaaaagccac ttgtcaatgg cgacagcttg ggaaatactt
     4201 gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt attagagcaa
     4261 ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc gcttatagcc
     4321 gatattaaaa caactgattt ggtggttttt gacgatattg gagctgaatt aggtagtggg
     4381 gtatcaaata gtaggcagtt tacaaacaac acgttaaaca cgctcttaga agcaagacag
     4441 aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga agcctacggt
     4501 gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa attccagcaa
     4561 acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa taccgtaata
     4621 aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag tattacgatc
     4681 tagccttgtg gcaagctgaa gcgaatggct ggaaagtaaa acttcaggaa agatttgagc
     4741 tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat attcccgact
     4801 tcacatttta taaaaacggc aaacttgtca aagtcgtaga tgttaaagga atgcagacaa
     4861 aagactttaa gatcaaggca aagctgtttt gtcatcaata tcaagtgccg ttgatattag
     4921 ctaaaaaata tcggaatacg ttcaaggaag agcgttttta aagaggtggt ctatcatgac
     4981 aacagaagaa gtgattcaaa tgcgaattcg aagcattcaa cgtgagattg acgatctgga
     5041 acgaacaaag gcagtgatgg tcaatgaaac ggcgagaaag gcaatcgatt tgcacataga
     5101 gaacttaaga agggaaattc gtagattgga ggaatgagcg tggataagaa agcagcaatg
     5161 aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga aatagttgca
     5221 gaagtccaaa agctcggtaa atcaatgtgg actgaaaaaa ccagacggag aatgccgaga
     5281 aaaattgcaa tctggcacgg tgaccgaatt ctagtaacag gtaccgctga acagttatct
     5341 gaaattactg gtctgagcaa aaacatcatc tgggatagag cgaagaatat ggatattgat
     5401 tctaaaggaa gacagtttag gtatgtggag gagaaataat gaatctcatt acacaataca
     5461 gtgatatcat cctcaagaaa atcatgatga agattcagaa agacaaaaaa tcaaaagaac
     5521 gagcggaatt agttaagttg gaaatggctg aaacaggggc aggagtgcga agtagcaggc
     5581 attggaaggc agcagcaaac attgaatttt attacaacga aattcaaaaa gggttcgatc
     5641 aaatgcgtga gctggatcgg caaacaaatt ggagcaagaa acttcatcaa gatcgtttca
     5701 aatttgtaga gaaatataaa gagatattag acgaatacat ggagggcagc aaatgatacc
     5761 gagatttcga tcgtggtaca caccatttaa aggtaaaaca attggacaag aaatgaaata
     5821 tgggcaagca ggaaggttga tcactcatgc tgaaatggct ccagataaat atgtgcttat
     5881 gcaatccaca ggactgaaag ataagaatgg cgtagaaata tttgaagggg atgtagttct
     5941 ggttaatgta agtaatggtt ttgatcattt agttaatgaa aaaacggttg tacaagaatc
     6001 cgagtttcat tccggattag tttgtaaatc attaattagt ggaatggaat atagaatatt
     6061 cagccaccaa aagatgggat acgaatatga agtcaccgga aatatacacg agaatagcga
     6121 gttattggag gtagagtgat tatcttagct actgactaca tcgattcttt aaaagatgaa
     6181 aatgggaatg tcccagcatt gctaaattgt ggaattaaaa gtaagaatag aatcattttg
     6241 ttacggatat cggctgattt agcaaaatat ctttatctga gcaagacagc agtatattac
     6301 actgcggcta cacgttatca gtacaaggga aaaacaatca gtcaggatta ttatgatcgt
     6361 ttaatcagtc ctgacataca tggaaaatca aaatcagcga tcaagttatt tggcgcaata
     6421 gagatatatg ccgatgattt tccaaattta tggttgaagg aggaacagta atgaaagact
     6481 ggttagaaga agcaagcgcc ttagttggct gtttcataaa ctgtgcgttt atatctgtaa
     6541 ttattgcact ggtgggattt gtatcattga aagtgattat attgctttgg cagttaattt
     6601 tttaggagga acagcgatga ataaacagga agcaatcaaa aagttagaaa gtattaaagt
     6661 gataggaaat gatgcactag ctgcttgcta taacgagagt ataaatgcag gtattacgtt
     6721 aatgaaaaaa atagacgaac cgaagaagct agttattcca caactcgtgg ccggttggct
     6781 tgagaaatct acggaccctt ttacgaaagc tgaaaaaata gcgtatttaa tcaaatctaa
     6841 agatggtgat tcatattatt tctgtgattg gtttgtacga gatggcatag tgacgcaaga
     6901 acaaggagaa gaattactcg cttgggcaac gagacaatca tatgaaacac tattgagctt
     6961 gtacaacggc tacgaggtcg agaaagagcc gttgtacagt gtaataattg ctggcgagta
     7021 ccttgtcaag gagatcagtt gcagtaatga agttaaacta gtatcaacag cttacctgcc
     7081 acaatacctt gcacaccact accaactgac cgaaaaacag atcaaagcaa ttgatgaaag
     7141 atactggcca tttgctgtgc cagtggaaga ggtggcggaa ggatgagtag acagataatg
     7201 ctccatatac cagacggaac aaaggcaatg acgattacca ttgttgctgc acaaaaagac
     7261 ggtgggttgg cgttgaaaac aaaaagcatt gataccaaac agattttaga aggcagagat
     7321 gtcgagatag agatcgatga ggaggaagcg gaatgaatgt tcaagtttat ttaaaaagag
     7381 atttggccag gtttcctcga agctgggata caaattccca cccatccgag gcagaagaag
     7441 agggctatct aatgtctgct aacatgatag aaattacaag agatgatgcg gatgagtttg
     7501 ataaaaaaag atcatggtac tcggttccga atccaatgta tacggctgta atagaagatt
     7561 atcatatatc cgacagattt attctgattg atctagaaag gcctaagaaa cgaattaaac
     7621 agtatagacg ttgtggattt tcaacaaaga aatggtgata acaaaacaga ggtgaagaaa
     7681 aatgaacaac agacaccgca gagtagcaaa actaagaaat caagaactga atgtagcgaa
     7741 agtaaagttc gaacgggagt atggtgtttc tgcagaagaa acatataaag tggcaagtca
     7801 gtgtgttgct gatgcaagtg atgctattcg taagtttgga atttcgatac tagatattaa
     7861 atgggaggat acagaatgaa gatttatgta gtaaagtttg gaaatcaatt ttacagaagc
     7921 gatgaacgtt ctataggggc taacacatta tccattgtag acatactcca aagcgcaaga
     7981 tggtttgata atctcgaaga agctaaccaa gtttcacgac gacttggtgg attaacgcaa
     8041 gtatatgaac tggtcactgt cgatcatgag ggggtagagt aatgaaacta aaagacggat
     8101 tttacgctag tagtcatggt atcggcggtt tgatgctaga tatgccgaca aagaacccta
     8161 aaacacgtga gaaaccaaaa ttcaaagtcg gtgacatggt tcgctgtgaa gcagaaggat
     8221 ttatctatcc atttcgtgga tatgtagaaa aggtattgtc gaactcagca atcattcgta
     8281 ttgaaaacac gatggaatgt gacaagtggt tagcgaaaag caaagagaat ttagctgtag
     8341 cgagattagt tgacatggaa gtaatcgaga gcaaataaaa aagccggatc gctccgacta
     8401 acataataaa acagacaagt ttattatatc acataaagga gcggtttgac ttgatgcaat
     8461 tgttacgaga ggtagatttc aaacagacaa gatgtaatgc gagagatgtg ctgaagaact
     8521 ttcggcgttt ggagcggatg gcaggtcgct ctttgataga tattaagtct ccaattatta
     8581 cggatatgcc gaaggcgccg aagcacggca ataaggcaga ggacgcgatc attcagatga
     8641 tggatataga agcagagaga gatgcgattt tagcggcttt gatggctctt agtctgatta
     8701 gccgtcagat actctactac agcttttgtg tgccagatag cttctcaaac tacagaatta
     8761 gccgtgaagt gggttattca gaaagaagta tacaacggat gaagtcggaa gctctaatag
     8821 agtttgcaga agcatataaa cacggaagaa taattgctta taaataattt ggcggttttt
     8881 tggcggaatg atggcggttt ttagctattt accagtgata ttatggtagt gtcgaaagat
     8941 tagtgatagg tctgagacaa aataataata aaaggaacat cgttttatta ttgtttcaca
     9001 attaagcttc gatagacagc aacggaaata ttaagaataa ggatgtgaat ttcaactcct
     9061 tctaaattgt tcttattatc tatcatccgt tgctgtctat tgttttttta attattcaca
     9121 cgataactaa aggtgagtga agagaaatga ttccattaat aatttcaatt tttgcgctct
     9181 gtcttaatgt ctatatgatt ggatttaaaa atgggcaaaa taaaaaatag tagcagccaa
     9241 gaataatttt atagtgtcac tgtggcggaa agggtagacg cttaaaaata aggtcaatac
     9301 gtcgagggat agccttaacg ttttatgatt tgaccatgca atgttcgatt cattgccagt
     9361 gactttggca accgagggtt ggaaatgggc gctcaaagta cacgagcaag gcgaggtcga
     9421 tagtaatcga tggaatcggt gtaggttgct tgatagagct ataactgcat ctcgtttatg
     9481 aggtgtagtt tttacagatt agatcactct ttgagtggtc tttttatttt acacaaagga
     9541 ggtaacaaga atgtatagac cacaatactt agaacagaag tatgaagtaa tcactgtgca
     9601 aaacggtaac ggtgagatag tacgaaagta tagaagacca ataaagagcg atacatacaa
     9661 acgaaaggaa agcaatgaag ttattccatt gtatggcaaa agaatagcta agcattaaat
     9721 aagattgcga aaggagacgg aatatgaccg aggaattcta tagatggcta ttacagttga
     9781 taagagaaga tcgtttagtt aagttctatc agtctcctaa atggcgcagg cttagagaga
     9841 aagcgatgaa acgagatcac tatgaatgcc aagagtgtag aagactaggt aagtatcata
     9901 gagtagaaaa cgttcatcat ataaaggaag tcaaggatag acctgactta gctttagatt
     9961 tagataatct tatttgttta tgtgttgaac atcataatga agttcatggc agatatctta
    10021 cagcattaga taaacaagag aagaagatag aaagctttgc taacttcgat gcaagtgaaa
    10081 ggtggtaagt gcatgatcat caatgataat ggcagagagt atgatacaga aaagattgaa
    10141 gagtattcat cttatactca gggattaatt aaacgtttga tatacgttcg ctatgtagat
    10201 attagggatc tgttatcaga taactgttgt agtaaataca aagtgaatca agtaagagaa
    10261 gcgttgaata aagataataa cgtcgaaaga ataaaaaatg tttttggata tagtattgaa
    10321 gagattaatt attacattga cttcgctgaa gctttcattc cgatggtgag ataacccccc
    10381 cttaaaataa atcgcaaatt ttttgggggt gatgaaacgg agggggctgt caggaaaaga
    10441 gattttttcg aactttatca tgaaaggagg gctaaaatgt ttaaaaacga attgtctcaa
    10501 aatcgctaca gagaaaaatt acgccgctct ttaataagcc aattggaaag tcagaaaaca
    10561 aatattgagc cattcttaga taatgttgat cgttatatca gtttatggga aacggcgata
    10621 tcactggaag aagatatatc cgagaacggc attagactgg agaatggtaa aaagaatgaa
    10681 tcagtagcgt tgcttgtttc tgtcaacaaa caaatgggat tgatgttgga taaacttgcc
    10741 attactcctg aattggtagg tgaagcaaat gaatcaattc ctgagttata agcatattga
    10801 aaattggttc aaagctatag aagaaggcac tatcaaggta tgcaaagagc aattattgct
    10861 aaaaaattat ctagaagaaa gagtctttac tagagaagat atttacttcg ataagcagat
    10921 ggtagaggat tcaatcaata taccagcaca atactttcca ttcgaattaa ttccgtggga
    10981 aaaatttcta caatgtttta tttatggtgt tcgatggaaa aaagataaaa cactagtgtt
    11041 caatagatat ctttcattaa tgggacgtgg taatggtaaa actggttttg cttcttggaa
    11101 caacttcttt ctactaaccg ctaaacacgg tattaaaaat tatgatattg atatctatgc
    11161 caataatgaa agccaagcaa agactagttt tgatgatgta tttaaagtaa ttaaagatca
    11221 tcctgattta gataaaaaag tatttaaagc tacgaaggaa gttattcaaa atatcgctac
    11281 aaacagcaaa cttcgttata acacggcaaa tgctagaaca aaagatggga agcgaccagg
    11341 tgcaaaccgc tttgatgaaa ttcacgaaaa tgaagattat tcaatgataa atgtggctac
    11401 ttctggtggt ggtaaaattc gagattatag agaattttat gatacaacta atggtcatgt
    11461 tcgtggtggt ccgcttgatg acattataga agaatcaaaa atgattcttt ctggagaact
    11521 tggaattgac aaggatggag cagaattttc tagtttgttt ccatttattt gtcgcttgga
    11581 taacgataat gaagttgatg atcccgacat gtgggaaaaa gcttgtccaa ctattaatta
    11641 caatgcagat ttaaaacgga aaatgtttca agaatactct caaatgcaac gtaatgctgg
    11701 tttaagactt acgttcatga ccaaacgaat gaacagacct atggaagata cacgatttgc
    11761 tgttgcttca tatgatgatg ttctgcatac gaaagaaaaa gaatttcctg aaaaaatgga
    11821 tgaagtgata ggaacagtcg attttgctga tagacgagat tttgccagcg ttgggttgct
    11881 aggaaaatac gataaagatg tttattttac acaacatact tttatccacg aatcagccct
    11941 tcgattacaa aacatcaaac gagaggttat agatatttct atagatcaag gaaaatcaca
    12001 gatcgttcat ggaaaaaata tagaagctga ttatattgta ggttggtttc ttgaaatgag
    12061 taataaatat tatattaaaa aaatcgctat ggatatgtac cgtgcaaaaa tattgaagcc
    12121 cgctttagaa gaagcaggtt ttactgtgga aattgttcga agcggatctg ttacacatgg
    12181 tatgttaaaa gatctggttg atgacctttt tattaatcaa cgtttatttt ttggtgacga
    12241 tgcgattatg cgttggtatt gcatgaatgt atatgaagag catatttcta atggaaatat
    12301 acgctatgaa aaaatagaac ctgaaactag aaaaacggat ggcttttttt cattccttca
    12361 tggtttgaat tttttagatg atatttatga ttctgctcct gtaacagtca caaatagctc
    12421 agtagaaaat acaggaactg gatttactcc tctagtattc taacttgaaa ggaggtgaga
    12481 aagtggggat ttttcaaaag gcggtaggat acttcacaaa aaaagcaacg gttactttag
    12541 aagaatactt ttgtaaattg caagttgatt ttgtgtatcg aaaatttgcg attgaaactt
    12601 gtattgattt gattgcaaat gcgatgagta aagcggaatt caagtcatat gaagatggaa
    12661 aaaataaaaa gaatgatctt tactataggc tgaatgtagc tcctaataag aaaaataatg
    12721 caacagaatt tagaaaaaaa ctgatcagga gattaatatt ctacaatgaa gtattgatcg
    12781 tttctccgtc taataattct agcgaaatat ttattgcgga tagttgggat gtcacagaat
    12841 atgcattgaa agatgatgtg ttttctcaag tgcaaattaa caacatagtc cttgatagag
    12901 aatttctaga aagtgatgtt atctatataa aatacgcaga tcaacaaatt aggcaactag
    12961 tcgatgcgta ttatcaagcg tatgggaaac tcatttctag tgccatgaat gtttacaagc
    13021 gctctaacgc tcgtagatac gtactgaaag ggaatttatt ccgaccgcaa gacaatacaa
    13081 cacaagatca aatcaataaa atgatgacat cacaatttaa ggcttttatg gaagctgata
    13141 atgcaggtgc ggtatttcaa ttacaaaatg agtacacatt agaagatttc agcggaaact
    13201 tccaaagcaa ttcaagagat ataaaaaact taatagacga catctttgag atgacagcag
    13261 cagcgtttca cgttccgaaa aacctactaa agggagacat gagtgggtta tcggatcaag
    13321 tggacgcttt tttaatgttc gaaatcatac cgattgctga acttattcag gatgcgttta
    13381 acgctagtct ctatgaagta gaagaatact tgtcagggaa ttttgtacgt gtggatacaa
    13441 ctatgatcaa gattactagc ttcaaagatt tggttgacgc tattgatgta ggcattagaa
    13501 atggagtatt tacaatcaac gaaggaagag aacgcgttgg aaatgatcgc tctgataagg
    13561 cgatggcaga tgaaatattt ataactaaaa acaatcaaca agtatcgaaa ggaggtgagg
    13621 cgaatgacga caatgaaaac atttctagca gtaaagaatg aaggcgcagt accgcaaatt
    13681 tttattcagg gatttattgg ttctagttgg ttctttgaag ggaatactga caagggaatc
    13741 aaaaatattt tggatagtct aggtgatcaa gaagaaattg aagtagtaat taattcaaac
    13801 ggtggagacg tatttcaagg gattgctatt gggaacttac ttaagtcaaa taaagcaaaa
    13861 gttaacgttg tgattaacgg cttagccgct agtgctgctt caattatcgc aatggctggc
    13921 gatactataa aaatctacaa caatgcacaa ttgatgattc accgcgcttc cacatacgga
    13981 gaaggaaatg tcgatgactt ccgtacgatt gctgaccaac tggaatcaat tgataaatcg
    14041 gtaaaggctt catataaaac acgattcaat ggcacagatg aagcattgca agaacttctt
    14101 gaaaaagaat cgtttatgga tgcagaaaca gctttgagtt atggattggt cgatgaaatt
    14161 atcgatgcag aaaatagctc aggtactgaa gctaaaaaag aacaaagcgt tgaagaaatt
    14221 ttgaatgacg ttaaagaaaa aagagcagaa aaaattgctg catttacagc agcattaaat
    14281 aaaacatttg gacaaggaga tgtaaaataa tgacagttaa aaatttaaaa ggtgtaacag
    14341 ctgcaagcga ccaattgatg aaagctttta aagatggtaa cgaagaatct tttagcgcag
    14401 ctatggtaag cttatctaag gaaattcagg ataaaatttt agaagaagca acagcaaaaa
    14461 atcaagatca attagtatta atgaaccgtg gtcagcgtgt attaactaca caagaaacaa
    14521 aattctataa cgaagtagtg aaaaacgaag gttttgcagg ggtcgaagaa ttagtgccag
    14581 ctactgtatt tgaacgtgta tttgaagatt tagaacaatc tcatccacta ttgcaaaaaa
    14641 ttacttttgt taacacaact ggtgtaacag aatggattgt gtcacgtgga gtcaatccag
    14701 catggtgggg taaactttgc gaagctgtta aaaaagtttt agataatggc tttgatgtaa
    14761 ttaacatgaa gcagttcaag ctatcaggtt atattcctgt atgtaaggca atgcttgatt
    14821 taggtccagt atggttagat cgttatgtcc gtactgtttt agtagaatca ttgagaattg
    14881 cattagaaca agcaattgtt gatggtactg gtaaagatat gccagtcgga atgatgcgtg
    14941 acatgagcaa acaaactagc ggagaatatg ctgaaaaaaa agcagaacct attacagctt
    15001 tagatgctgt aactatgggc ggtttgatgg cgcgactatc aaaattcaat atcgaaggtg
    15061 tgaatgatcc gatttatcgt aatgtgaatc cttctgatgt ggtcctaatt gtgaatccaa
    15121 ctgattactg gtctaaagta ttcccagcta agactgtact aactgctaat ggagaatacg
    15181 tacaagtatt gccagtacca gtttcagatt tgcagtcaac ggctgtgcca gaaggaaaag
    15241 cagttattgg ggtagcctca gattacttca tgggtgtagg atctacacta aaaattgaag
    15301 cttcagatga ataccatttt gttgaagacg aacgcattta tctagctaaa caatatgcaa
    15361 acggtcaacc taaacgtaac gatagtttca ttgtattaga tattagcgct ttgggaacta
    15421 ctactacaac tacaaaacca acaaccacaa caactacaac acaagcgtag gtgatcagaa
    15481 tgaagtatat tctttgtcag ccggcaatca atcggtttaa atgggagctt gaagtttgtt
    15541 taactaatct gaagaaacta ggaatcaaag atatcgtatt gcttttcagc agacacgatg
    15601 atcagattcc tatttttttt gagaaggaat atggtgttga agttcatgtg tacgatgatc
    15661 tgcgggacga caaagagtat attccttcga ttaaaccata tttatggtgg aaatatttag
    15721 aagaagatca ttcgcgtgag gacgaccgat atttctatat cgattcggat gtcattttca
    15781 ataaaagaat taatttgcgc aaattgcctt ctaaagatga tgtttggtat tgtagcgact
    15841 gctgtagtta tctaagtctt gattatatta gaagctgtga aaacggagaa aatattctaa
    15901 aagatatggc aaacattgta aatgttacag tagaatcttt ggaaactata aacactaatt
    15961 caggaggcgc acagtgggtt attaaccgcc ctaaagcgaa ttattggaaa aaggtttatc
    16021 tggattctaa tcggctatat cgctacctta gagggcaaaa aacaaatata aaaatctgga
    16081 cagccgagat gtgggcacag ctttggaaca tgatgtattt caatattggt cctaaagttc
    16141 acgaggaatt agacttttgt tttgctactg atccaataga aaaagttaaa gaagtaaaaa
    16201 tcttacacaa tgctggagta acaacaaacg atgaagattt atttttcaaa gggagatacg
    16261 tgacttctac gccttttgat gaagatttat catttgtaaa caagaaaaaa tgctcttacg
    16321 catatgttaa agcaattaag gcggtggtta gatgacgcct gaacaagtga ctgaagaatt
    16381 gctaatagct gtgaaggata atatttacgt tacctggaac gaagaagatg agtcaattaa
    16441 aaagatgata gctaaaaatg ctgtttatct tcaaagtaaa gtgagtacaa cactttcttt
    16501 ttctcctgaa agcttagaat acggattgct aatcgaaaga tgtagatacg actggaatcg
    16561 tgctttagat gagtttgaac aaaatttcgc tagtgagtta ttaggtttca ttcaacatta
    16621 tgcgctacaa gaatatattg caggtgatgt gaatggcgaa taatcgtaga ctcgaagaaa
    16681 cattcaacga tggttggtta aagattttga cgcaaactac caaaagaaat gaactaggaa
    16741 aaaagattgg tgtagaagat acagaaatca cttctttaaa atttagaaat ctttccatga
    16801 gagatagtga tataacagct atggatgcga tgggatcgaa attaactaag aaagtaaaga
    16861 ctccatttca tccaatcgcc aagaaattta ataaagatca atattttatc gtaatcgata
    16921 gtatgcgtta caacgttatc tatgccgatt acgataattt ttatatctat ttttatcttg
    16981 aaagtgtggg tgaatatggt gattgataat tctaaagaaa aagaacgttt aaataagcaa
    17041 atttctgcta tcaaaacttc cttagaagag cattttggcc tcaaactctt tcaagactcc
    17101 gtaggcgagg atgagctacc tgatgatttt aattacttca ttctcgaaac aggagaaata
    17161 gaaatgatca ctgagccaaa atatagcgtg ggtcaaaatc tatatctaac tttctattca
    17221 gaaaatagag aagatttaac aggagattca ctagatatta tttcattgat tcaaaatcgt
    17281 tcgattcgtt ttcagagaat ggatcccaat catttaaaac tagagaacca agatcgctat
    17341 atcgatcaat tggtatttac gtttagacga ttattgaaga gtgattgtca tggctaaaaa
    17401 tagttgggag ctaaaaataa atggacatga tgaacttctt gtgcggatgg aacgctattc
    17461 aagcgagagc gaacgactga ttaacgaagc attgaaatca aaaggttcgg ctattgcagt
    17521 ggataggatt acagaaaaaa ttcctgtttc tgaagcagat ttaagaagag ggcaccaaca
    17581 cgcaaaaaat agtcgtccac ttaagactca atacattaat ttgggtttca tcattagacc
    17641 tacaagaaaa tttgagtatt taaaatatcc tgatttgggg ataggtactt ctaaaagaaa
    17701 tcagccggac gaatttatga gaagaggatt aggtcttgca cttgatccaa ttacagaact
    17761 tctgattcgt caattcgata aattaaataa ataggaggaa caacaatggc taaaacaaca
    17821 acagtaacaa cttttgacaa tataagtatt aaaagaattt cttttaattt taagaatgca
    17881 acaaatgcaa tttcaactga ttgtaatgga caattagatg gcgaaacaga aatgcagaga
    17941 attgtaaaaa aatgcggttc aacagaagta aaatcgaaat ctaaaccaat caatatgacg
    18001 gtaacaatta ctgcacatgt accgatggaa gtttatcgac gcttcaatgg gttgaaacaa
    18061 gatgaacgta ttaaatcggg aatttactct tatggtcctg attccgtagg ggaagatttc
    18121 tctcttgctg cagagatcgt ggatgatttc gaagaaaaaa ataagctgat tggtatgtta
    18181 gcatgcactt cgaatacagg attaacattc tctattgaaa atggtgcgga tgaagtagct
    18241 gcgttagaac tagaaacaaa agttatgcaa gatgaatttg gtaaatttta tcatgaagca
    18301 attgttgcag aacttgaaga agacttaaca gatcaatgga tgacgaatct atctgctgat
    18361 gtgattaaaa aaacttcagt tgtgacaact acggccactc aatcacagta aaaaaaacgg
    18421 aggtagcgaa atgaacgaag attactcaaa aattgaacta aacgatggaa caattttgaa
    18481 tttagaacct aaactgaata tcaagaaatt attgatgatc aatagagatt ttaacacaga
    18541 cgagtttgca aaaatgtcga tgggaaaagg ctctatggat attacagtta ttcaaggtgc
    18601 aaaagccgta tatgtagctt atcgtcaagc gaacatggtc gattacattt cattcgatga
    18661 atttatagat aaatgagatt ttgatatgga ggttgcagta gctgtataca gtactatgat
    18721 gttcaaacaa gcacgtgatg cctatcaaaa agaattcgaa aaagcaaata aggaaaaaaa
    18781 gcttcaaaag taaaaatgcc aaagctctta gttgaaacgt gggtcgatgt ctattcgatg
    18841 ttgaccgacg ttttttctat gccttcagat ttggttttaa gcgatatctg tttagatgac
    18901 attttgcaaa tggcttacaa caagagcgct tatgaaggat ggaagaacta tgcaataaat
    18961 caatcccaga aaaactaaag gaaaggaggt aaaaaatggc taaaaagaga acggaagcag
    19021 aagtaacttt catagctaac gatgacggat tgaaatctac gttaaaagaa atcagtgctg
    19081 aattaactaa aaatagagca gaattaaaac tagaacaagc tcaattgcaa cagactggtt
    19141 ctgaatcaga caagttagga agtaaattat cttctttaga aaagcagtat gaattacaaa
    19201 gtcaaaaagt tgaagtaact agccaacgtt tagccaatgc gaaaaaatat tatggagaaa
    19261 attccaccga agttcagaaa cttgaaagag aactgattaa tcaacaaaca gcgcaacaac
    19321 gtttgtcaaa cgaaattgat aaaacgagta atgcactagc tcaagcaaaa ggcgaaatac
    19381 agacgtacga gtctacaatg caacagttgg atagcgaaca aaaaaatgtt caagctagtg
    19441 cttctctgat tgaatccgaa tacaaaaaat ggcaagcaac tgctggtcaa tcagcttctg
    19501 aagccgagaa attagcgaaa gcccaagaat atgtttctca acaatctgaa aatgcggaga
    19561 aaacgataga tatcctaaga cgacagttag aagctacaca gtctgaattt ggcgctacat
    19621 ccacagaagc aatgcagatg gaagcgaagc ttaatgatgc tgaacgtgaa tttgaagagt
    19681 taggacaagc tgctaaaaat gtagatacaa ctaacttgga cgatatcgga agcaaaatag
    19741 atatgaataa tttaatggaa gcttctgacg ttttaagcga cattggcgat aagcttacag
    19801 aattagggaa acaagcagtg gactctgcta acagtgtagg tagttcccag agtaagatac
    19861 aagctaattt tggtttgtct aaacaagagg ctgaagaatt aacgaatgta gccagagaca
    19921 tttattataa aggttttgga gaatcgttag atcagtccac agatgcattg attttggtaa
    19981 agcgtaattt aggagattta aataatcaag atttacaaaa tatcacggaa caagctatgg
    20041 tcctagaaaa caccatgggc gctgatatgg atgaaacgtt acgtggtgta aatggcttaa
    20101 tggtcaattt cggcttgagt gctcaagatg caatggattt aatggtttcg ggtactcaaa
    20161 acggtttaga taaaacgcac gaattaggcg acaatatggc agaatatagc caattatgga
    20221 gtcaaatggg atattcagct gatgaaacgt tcggaatgct tcaaaatggt ttagatgcgg
    20281 gtgcttataa ccttgataaa gtcaatgact tagttaagga aatgggaata tcgttaacag
    20341 atggtcgatt tgagcaaaac atggatatgt ttagtgaaag tactagaaaa gcttttgaag
    20401 agtggaaaaa tggcggagga acacaaaaag acgttattaa ttccatgatt caagatttta
    20461 gcaatatgga tggtcaatac gaccaattaa ataaagcttc gacaatttgg tctgcgcttg
    20521 gcgaagataa tgcgatgaaa gttgtccaat ctttaactga tgttaaccat acatttgatg
    20581 atgttagtgg atctgcacaa aaaatgaatg aagattctac tactccgtta caagaattaa
    20641 acggaaaaat agctgaatta aaggattcat tagctcctat aggcaacaca atcatagatg
    20701 cactcgaacc agtaattgat tttctaggaa agatggctga tgcgtttaat aatcttccac
    20761 aaccagtaca ggattatgcc gtagcgattg gcggattgac tgctgcattt actttattaa
    20821 tgccaataat agttggcttc atggctctag gtggtcctac tacattaata ataggagcag
    20881 ttattactgc tattgctgga gttatagcaa ttataaaaaa ctggggcgca attactgact
    20941 ggtttaaggg aatatggagt aaattcactg attggttggg tggtacttgg gaaagtataa
    21001 aagaaggtgc ctcatcagtt tgggatggag ttaaagaaac ctggtctgga tttgtagatt
    21061 gggttcaaga tatttggcaa ggagtttctg attggtttgg agagctatgg agcggattag
    21121 ttgaaggagc ttccaacatc tggcaaggag tccaagaaac ttggcaaaca ttcgttgatt
    21181 gggtttcaaa tatttggaac ggagtcaaag aagtatggtc gattatttgg gcagacattg
    21241 taggaattgt tcaaatacca tggacattaa taacgtcatt gattcaagct ggtattaata
    21301 ttatcgtggg tatttttgat gtagctggac agttattagg cgcagcttgg caagctgttt
    21361 ggacacctat ttctgatttc cttaaaaata cttgggatac tatgacacaa tggataagca
    21421 tcgcttggaa cggaattgta actacattcc atactatatt tgatccagta gtggcatggt
    21481 ggaatggtat atggacatct attagtacta cggcttcaaa tatttggaat tcaattagtg
    21541 caacagcttc tagtatttgg aacagtatca agaatacaat cactagcttg gtacaagcag
    21601 ctgctacagt aattcaaaat atttggtcaa ctgtatctag ttggttaggt ggaatttgga
    21661 attcaatcag ctctacagca tcaaatatct ggaatagtgt gactagtagt ataagcaatg
    21721 ctataaacgc agctaaaagt gccattcaaa gtgtttggaa tagtatatct tcgtggatca
    21781 gcggaatttg gaacggtatc aaaaacactg ctttgaatct ttggaatgga attacaagca
    21841 ctattagctc taaagtaaac gatggaaaaa atgcaatttc aagcggttgg tccaatctaa
    21901 caggtattgt ttccgacata ttcaataatg ttaaaagtac aattgctaac atttgggaag
    21961 gtatcaaaaa gactgttagc gctccgattg attggattag agataaaatc agtggcatct
    22021 ttgataattt gaatatttcg ataccacata ttccgttacc acattttaaa ttgagtgggg
    22081 aattcaatcc attgaaggga aaaatcccaa cgttgggtgt tgattggtat gcgaaaggta
    22141 gtgtgtttaa ttctccgaat attatcggtg tcggtgaagc aggacctgaa gcagttttac
    22201 ctttgaaaag atctgttttg caagaaattg gtgatcgtat cttgagtagc acatcagttt
    22261 catctagggc acaaacgatt caacctgtga ataactacga attcaatttc acaattgatg
    22321 gtaacgcaga tgaggttact atgaagcaaa caactcaaca aatcattgat agcattacaa
    22381 aaattcaaaa tgataatgct tcggcatggc gttaaacagg agagtatttc tcctgttttt
    22441 ttagtattaa aaaggatgtg aaaaaatgac tgattgtata cattctataa tcgatggatt
    22501 tcctgattat ttgcataaat tggctttagc tgaaagacca accatacctt ctccaaaaag
    22561 acagagagtt gaaacttctg ttttaggtag gttaggtggc ttagtacaag attactcgtt
    22621 tgaagatatg tcgtttacat tgcactataa ctatttagag gatgtggaag accatcaagc
    22681 gttcaagcaa tcgttttata tcatgcgtca ttggttaaac tatgcaaaga aattagaatt
    22741 ctctgacgat cccaacgtct attacgttat ccagactatc gatattgggg atgcagaaaa
    22801 cgatattgtt gaatggggag agttcgatgt aaatattact gcgaaaccat tcgcaagagt
    22861 tcaagaagat gtacctataa ccgtagataa accacagtca tttaacttgc tgaataatag
    22921 tttagaagaa agttttccaa agattatcat cactccttca gctacttcat gccagttcat
    22981 cttaaatgat tatgtgttta gtttcgaagg cttagtagca ggaactgatg tagtcattga
    23041 tagtgatttg atgctttgct acgaagagca atcggacgga gatattttag atcggtccaa
    23101 caaaatgaag accatgcaat atccaacgtt gcaagtggat attaatcatt ttaattgtac
    23161 tggtttgagc aaaatacaaa tttatcgtaa cgggttaagg taggtgaaat agatgatcga
    23221 taatttaata actatttacg ataaaaatga cgcgaataat ttagctgaac atttatatga
    23281 tacgcaaggt ttaggcgctt tgtcagactg gttaacagct actgttagca ataaactaaa
    23341 cggagccgag atatttcagg gtacttattc aataaacgga actaatgcag atttgattgt
    23401 agaaggacgt attattcagt gttatgtaga tgaaaatcga gcaaaacagc gtctacggat
    23461 ttattatgca aaaacttctg taataggcaa tacgatagaa gtaaaagctg aacctatttt
    23521 caatgatata agaaaatcgg tgttgaataa atatgacagc ggaacagaaa agatcactgc
    23581 tactcaggca tggcaaaacg caaaagtttt agcgaaacca gctatccctt cgcagttttc
    23641 tttctcgtca ttagtagata cgcttgctaa tgtgaagata gaaaaggcga attttttaga
    23701 attctttggt ggaaaagagg gatctattct agatcgattt catggtgagt ttctaaaaga
    23761 taataacaca ttacgtcatg aaaaaagtct aggtacggat cataaaatca aagcgattta
    23821 tactaaaaac ttaactggtc ttgacttaga gattgatgct caaagtgttt tagttggagt
    23881 ttatccattc attagcagct cttcagaagg agaagacgag atcactctac cagaagaagt
    23941 tattttcacg gattacgtgg atgattatcc tgctggatat gtttcttttg ttgattttaa
    24001 agacaaagcg actgatgtag ccacattaag ggaagctgct aaagactggt tgaaaacaaa
    24061 catagataaa caaaaaccac aagtgagtgg ttcgattgaa ttagtaccat tgaggcatca
    24121 aagaggctat gaaaaatttg ttgatctaga aaaagtttcg atgggtgacg gagtagatgt
    24181 gtatcatcca cagttaaaag tgaatatgtc agcgagaatt gtggaatata cgtttaatgt
    24241 tctaaccaat tcatacgata aattagttgt aggaaacgtc aaaacaaact tcttagaaaa
    24301 cacagagaat aatgtcagca atttgattaa tgatgccatt gatcaattga aaaatggtgg
    24361 cgaaatcagt gatttaatca atgatattgt agatcatcaa actgatatga ttactggtca
    24421 aaatggtggt tacgttttat tagatcctaa agaagcccct agtcgtattt tgattatgga
    24481 cacaccagat aagaatactg cacggaacgt tttgcaaatc aacaacgctg gtattggttt
    24541 ctctaaaact ggcattaatg gaacgtatga caccgcatgg acgttagatg gcggattcaa
    24601 tgcctcgttt attacagctg gtgagatagt agggattact attagaggta ctacattaat
    24661 tagtgatggc actgattata gaacaagtat agctaatggc aaaatgactt ggtactcaaa
    24721 aaaagttaac aaagatatta tggagctaga agcacgtgat tatgtaagtg ctgatgcagg
    24781 tattgtatca tacaccatga aaactggtgg tggtttcatg attagaaatc cacaggggaa
    24841 tttggttttt agtacgtggg ataatggtaa taacaaaccg tttttatctt ttggtgcgcc
    24901 caatttcagg tatagcaatg ctagttatat aactgatggc gatggtagtt ctttaagcat
    24961 taatggtagt gcgggcagtt catgggagtt taaagtagct ggtaggacta tgaaatttac
    25021 tagtgatggt atgctaacgt tgccaggttg tttttttggt tcatgggaag atggaaaact
    25081 tgctaggttt gaacaatcaa cggtacaagt atataaaaat tttactgtta gaggtactaa
    25141 aaactcaacc gtaccaacag aacattatgg acaacgacta ttaaacgctt atgaaactcc
    25201 agaatattat ttcgctgatt atggggaagc cgttacaggt gatgatggta aagttcgtgt
    25261 tgatattgat cctatgtttg ccgagacagt aaatctaagt cgatatatga cacatgtgac
    25321 acctacagag ctagttttgt gtgctgttac tcatgaagat attgaccatt tcatcattga
    25381 aactagtaag ccaaacgtat tggttagatg gaatttagtg gcacatcgac tagggtatga
    25441 agatgttaga ttaaaagagg atacagcata tgatagcaca gtgcttgacc aaaaacgttt
    25501 ttaaaacgaa gacaaggagg tatataaatg gctagcagtt tatataattt ggctttagat
    25561 ttcagcaaag aattaaacta caccaaagct attatggctc gtcaaggtga caaaggaatt
    25621 acggtgactg ttaaaccgtt tttaaatggc ttgcagatgg atacgagtgg cggaaccttt
    25681 acattaaaag gaacaacacc atctaaccgt tacgtagata gtgtcgctac tagtataact
    25741 agcgaagaag tcacattttc tcttgatgga acatttatga gtgaagcagg atactataaa
    25801 cactgctacg tagaatatag aaaagataat caaattttaa cgacgcaaga tatcattttt
    25861 ttctcactag gagtgtctga catttcgcaa ggccaagccg atgaatatgt ttcgcaatta
    25921 gaagaattga ttcgaaagta taacgaaact tttgatgctt ttctggctga aattaaaggt
    25981 agaatggata gcttaaataa acagattact gatttaactg gtcaagctaa aacactacaa
    26041 gacaagttag atgctctgaa agaagaaatt tctaaattag gtaacttaca tgtgatgtac
    26101 tccaacagca tcgacttcgg gggctatgat tatagtggga atccgaattt aatgtccaaa
    26161 ctaaaatcga gcgattttaa cgttggttac cacgggtcac taactaatgt taacggaatg
    26221 ttacatttta tgtctgatgg tacaggggac attgatatgt ttacgcgtat taatacacct
    26281 cagcttgcta gtgggaaaac ctatactctg agtgcgaaag ttcgatttga tgaaggaact
    26341 acaggagcta ttgataaatt acgtttggtg tatcgtacat caccaggaga aaagatatta
    26401 ttggaagcaa atagtacaaa tattacaaca gatgatgtag ggaaagaaat aacaatcaaa
    26461 ggtacagcta acgttaatta tcaaatcaca aatttagatc gattttatat tagtgttagt
    26521 ttttctaaca gggataaaat aaatggtgga tttaaattgt acgacatcaa aatcgaagaa
    26581 ggctcaacag ccactccgta tcagccaaac ttactagatg acccttactg ggtaggtaaa
    26641 acatcgttgg gtaagaatat tgcaaataaa ggtacgacgt tcccaattaa atctagcgcg
    26701 tacgaaatat ataaaggtaa cacggaagaa gagcttataa taggtcaaac gtatactatc
    26761 acgcttaaag gaacaaaacc cgcaagtcaa acctttgtag catataatta ttggaacgtt
    26821 aattttggag atctaaaacc agttgaggga ttgacagacg tatggtctct aacattcaca
    26881 ccaacgaaac ttgaaccggg tttgcctaaa gaccttcgca tttttcagtc gcctaaagaa
    26941 acggccggcg catgccgaat tgactggctc aagatcgaaa aaggcgacac acgaaccccg
    27001 aatattgagc aatataaata ccgaggaatc ggcatgcgag actcaaacaa tccaaaagat
    27061 tatgtatggg atctagaacc tagctatgtt gagaataacc tagctacaga tatcaaggtt
    27121 gctgaaatca caggtaaagc aaacaattat accgatggga aagtatcgga gattaattcg
    27181 cagttgactg cttcaattaa tgaagtagac accacagcta aggatgctca aacaaaagcg
    27241 aatgctaatg cgactgctat agatgaatta gacaataaga tcgatgaacg cattaatgat
    27301 acagctacta ccacattaac agttacaaac gggaataccg gatcagcaaa gctttatcgt
    27361 gaaggaaaaa cagtttctat atattttgtg gctttaaacg gaaaacgcag tggtggaaat
    27421 gattcaacga tactaacaat tccagaaggc tatcggccac caattagttt tgagcaactg
    27481 gttggctcga tagaccgttc tactttgaac agtgctcagt tatctattgg tgcagatgga
    27541 gccattaaat ggcgaagaaa ctcaagttat ggatcggatt atacctttgc aattacttac
    27601 acgatttaga aaagcgtgaa tcgatatgaa ggcagcatat agaccaattg aaccttacgg
    27661 attcgagcaa atcattgtga atgatgaaga acatttaccg gaagaatgca cagaagtcga
    27721 accaccgatt ccaaattgga aaccgaaatt caattactgg gagggaaata aatgaaaaac
    27781 atttggaaat atggacgtac tggcggagag tacgcaggaa aagtattgga cgacatgctt
    27841 gtatccgttc cttacacaga tcagccaccg cttgaaggaa ttcgtgctga tggcgaaccg
    27901 ttaacaatcg ctgatcaaat gtttgatcct aaattgaatc aatggattat tttagcgaac
    27961 gcactagatc acaacgattt aaacaatctc aaagcaatgt atgagtcgtt agaaaatgag
    28021 aacggcgatt taaaacagat caatgccaaa ctcatgctaa gcgatgtagc aattaaacag
    28081 gaaaatactg cattgaaaga aaaagcggat agtttagcac aaatcaattc aaaaatgatg
    28141 cttgcttcgt tacaaaatag caaagacatt tcagaaatta aagagcaact aaatccagct
    28201 tcaaagggag gtgagtagta tgtttagttt tagcgatgtg aaaatgatgt atgattgggg
    28261 ctgtttcact aacgaacaag taatggtttt cgttccgttg tgcattactg aagaaaaagc
    28321 agataaaatc attagcaaag aagagagcgc atcttaattg atgtgctttt tattttgatt
    28381 caaggagttg tcacatgatt aatttagggg aatggggagc gatagcagga tcaataaccg
    28441 ctatcgtttc tttgatttta ttagtaataa aaccaattac tgcatctttc tcgaagatta
    28501 ctgagactct ttcaaaagta agtcacaatt tagatttgct gactaaagat ttagaatcga
    28561 gcaaatcaga tcgattgatg attcatgaag aactaaagaa acacgatgaa agattagata
    28621 cacatgcaga aaaattggta gaacacacgc aacaaattaa aacttt
//
LOCUS       DANKHL010000008        28084 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000008 DANKHL010000000
VERSION     DANKHL010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.010"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..272)
                     /locus_tag="PW101_000267"
     CDS             complement(<1..272)
                     /locus_tag="PW101_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2512699.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGING"
     gene            complement(274..471)
                     /locus_tag="PW101_000268"
     CDS             complement(274..471)
                     /locus_tag="PW101_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HDL2512700.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(865..2592)
                     /locus_tag="PW101_000269"
     CDS             complement(865..2592)
                     /locus_tag="PW101_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HDL2512701.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2585..3778)
                     /locus_tag="PW101_000270"
     CDS             complement(2585..3778)
                     /locus_tag="PW101_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2512702.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3965..4609)
                     /locus_tag="PW101_000271"
     CDS             complement(3965..4609)
                     /locus_tag="PW101_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2512703.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4703..4837)
                     /locus_tag="PW101_000272"
     CDS             complement(4703..4837)
                     /locus_tag="PW101_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDL2512704.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4839..6971)
                     /gene="feoB"
                     /locus_tag="PW101_000273"
     CDS             complement(4839..6971)
                     /gene="feoB"
                     /locus_tag="PW101_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDL2512705.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6968..7441)
                     /locus_tag="PW101_000274"
     CDS             complement(6968..7441)
                     /locus_tag="PW101_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDL2512706.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7842..8066
                     /gene="nrdH"
                     /locus_tag="PW101_000275"
     CDS             7842..8066
                     /gene="nrdH"
                     /locus_tag="PW101_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDL2512707.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8225..10384
                     /gene="nrdE"
                     /locus_tag="PW101_000276"
     CDS             8225..10384
                     /gene="nrdE"
                     /locus_tag="PW101_000276"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDL2512708.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10434..11399
                     /gene="nrdF"
                     /locus_tag="PW101_000277"
     CDS             10434..11399
                     /gene="nrdF"
                     /locus_tag="PW101_000277"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDL2512709.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11488..12627)
                     /locus_tag="PW101_000278"
     CDS             complement(11488..12627)
                     /locus_tag="PW101_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2512710.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12936..13649)
                     /locus_tag="PW101_000279"
     CDS             complement(12936..13649)
                     /locus_tag="PW101_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDL2512711.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13903..14604)
                     /gene="nagB"
                     /locus_tag="PW101_000280"
     CDS             complement(13903..14604)
                     /gene="nagB"
                     /locus_tag="PW101_000280"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDL2512712.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14838..16370
                     /locus_tag="PW101_000281"
     CDS             14838..16370
                     /locus_tag="PW101_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2512713.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16561..17763)
                     /locus_tag="PW101_000282"
     CDS             complement(16561..17763)
                     /locus_tag="PW101_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2512714.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17845..18534)
                     /locus_tag="PW101_000283"
     CDS             complement(17845..18534)
                     /locus_tag="PW101_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2512715.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18640..19008)
                     /gene="rplL"
                     /locus_tag="PW101_000284"
     CDS             complement(18640..19008)
                     /gene="rplL"
                     /locus_tag="PW101_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDL2512716.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(19061..19564)
                     /locus_tag="PW101_000285"
     CDS             complement(19061..19564)
                     /locus_tag="PW101_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDL2512717.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19768..19895)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19974..20663)
                     /gene="rplA"
                     /locus_tag="PW101_000286"
     CDS             complement(19974..20663)
                     /gene="rplA"
                     /locus_tag="PW101_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDL2512718.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20767..21189)
                     /gene="rplK"
                     /locus_tag="PW101_000287"
     CDS             complement(20767..21189)
                     /gene="rplK"
                     /locus_tag="PW101_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDL2512719.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21532..22200
                     /gene="sdaAB"
                     /locus_tag="PW101_000288"
     CDS             21532..22200
                     /gene="sdaAB"
                     /locus_tag="PW101_000288"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDL2512720.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22213..23085
                     /gene="sdaAA"
                     /locus_tag="PW101_000289"
     CDS             22213..23085
                     /gene="sdaAA"
                     /locus_tag="PW101_000289"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDL2512721.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23176..24195)
                     /locus_tag="PW101_000290"
     CDS             complement(23176..24195)
                     /locus_tag="PW101_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL2512722.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(24368..25273)
                     /locus_tag="PW101_000291"
     CDS             complement(24368..25273)
                     /locus_tag="PW101_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2512723.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(25332..25877)
                     /gene="nusG"
                     /locus_tag="PW101_000292"
     CDS             complement(25332..25877)
                     /gene="nusG"
                     /locus_tag="PW101_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDL2512724.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25978..26148)
                     /gene="secE"
                     /locus_tag="PW101_000293"
     CDS             complement(25978..26148)
                     /gene="secE"
                     /locus_tag="PW101_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDL2512725.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26172..26324)
                     /gene="rpmG"
                     /locus_tag="PW101_000294"
     CDS             complement(26172..26324)
                     /gene="rpmG"
                     /locus_tag="PW101_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL2512726.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26486..27130
                     /locus_tag="PW101_000295"
     CDS             26486..27130
                     /locus_tag="PW101_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2512727.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27186..27689)
                     /locus_tag="PW101_000296"
     CDS             complement(27186..27689)
                     /locus_tag="PW101_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL2512728.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27702..27748)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT         8500 a         5804 c         4965 g         8815 t
ORIGIN      
        1 ccatttattc cagcagttct tccaatttca ctcaaatttc ttattaattc ctcttgatat
       61 tcagtgaatc ttattttatt agtataagca gtaaaatata gataattcac caatctattt
      121 attaaaggac cgggatctaa ttcatatttt tgtttttcta attttaaata taccttttgt
      181 aaaacctcaa gaatatcaga atatttagga tctttactta tagatatatc attatataat
      241 tcatgaacaa tttgcttagc attttttttc attttagcac ttccctggaa ttgctccacc
      301 taaaaaacca cctatagaca ttcctgcaat acaagtagtt gccttggccc aatcgaccgt
      361 acatttattt ttagtgcaat acactccatt tccataatat tttccactat gagtggtacc
      421 cccatagata agttgtgtct ctttaataga caaaattttt aaatgtttca taatctaatc
      481 acccctaaat taatgttttt agactattat tctaacattc attaaatttc aaaaaacaat
      541 tccttcttga aacgtagcaa aataattcct gaataagaat ttaatcactt ttaagtatat
      601 aaactatgta tattctttaa tattttttga gaatgcactt cgtacataat aaactttaat
      661 cacattagtc tttcatacct acgatcgatc tctttgactc gcacccataa attcataata
      721 gtttgtcggc gtctttggac gtttcgaaga tgagctacgc attctctttt tttatagaca
      781 aaaaagatag agacgaacat ggagcttcca aatttggata tttgatccaa ttttggaagc
      841 tccatttatt tcgtgtctgt ctttttattt tttccagctt tcgactaatt cttggttctc
      901 tttgatccag tcttttgctg cttgctgtgg atcttttcca ttgttgattt ccaacataac
      961 tttttccata tctttttctg accaatggaa attgtctaat actttgtaag cttctggctg
     1021 gtcttctttc aatccttttc gaaccatcgt atggatctgc tcttcgcttc ccattccgtt
     1081 ttcaggatct tctaagtatt tcagatcata tttggcaaac atccaatgag gtgtccatcc
     1141 tgtaactaca attggttcgt gttttttgat cgcttgacct agtgcaacgg tcattgctcc
     1201 tgaagaagat gtctctactt tccagtcttt caagttgtca tatttttgga tcgtgttttc
     1261 agcagccgta actacaccag ctcctggctc gattcctatg attttcttac ctgcctgatc
     1321 ggttaactca tcgatcgaat ttacatccat gtaggctggc actgctaaac caacttttgc
     1381 tcctgtcaaa ttggcaccta ggtcttcgac ttgatcttta tattgtgcat attgtgaacc
     1441 atgagtttta ggtagccaag ctgaaaccat ggcatctgac tcgccatttg aaacagattt
     1501 ccacatgatt gaattatcta atggcgtcat cgttacatca tagcccattt gtttcaatac
     1561 ttcaccgact acatttgttg aagcaacttc tgtatcccat tcaacataag ataagttgat
     1621 acgttttgtt tctttggcag atgaaaaact ccctatacca atcgcaccta agatcactac
     1681 tactacagca ccaatgatca gtccttgacg ttttttactt ttctgtgttg ttgctcctgc
     1741 agctttttta ttgtttggct ggttcaaatg ctgtgtaaaa cggtcaatga tgattgctaa
     1801 gataactaat gcaaccccgt tgacaaatcc attcccgact tgtgctcttt gcaatgctga
     1861 taatacgccg cgtcctaagc cgggtgcacc gatcatagag gcaataacga ccattgaaag
     1921 tgacagcatt gttgtttgat tgatccctgc catgatcgta cttttagcta aaggcaattc
     1981 tagtttgaat agtttttgac gacttgtgct cccgaatgaa tcagaagctt ctactaattc
     2041 tttaggaact tgtcgaatcc ctaaattcgt aaaacgaacg gttggtggca gagcaaagat
     2101 gactgaggca aatacccctg gaaccatccc gataccgaaa aatgcaactg ctggaatcaa
     2161 ataaacgaat cctggcattg tctgcataaa gtcaaggatc ggtttgataa tactttgggc
     2221 tttactgctt tttgccatca agatccctaa tggtacccca atgatgatcg agatcacact
     2281 agataaaaga acaagtgtga cagtattcat caaatcattc catagacctt ggttataaat
     2341 aaaccataga ccgattaatg tgaacaacgt aagtcctgga cgtttcttag aaataaagaa
     2401 agctgctacc gtcaataaca agataaacaa aggagctgga atcactaaca gtaaatttgt
     2461 aatccccgac atcactgcct gtccaatcgt ttgtaaaaat gaaaataggc cagaaaaagt
     2521 tgtcgtaaac cattctgtga tattttctac ccagtttgcc actggtaatt gatagttcaa
     2581 tagattattc atgttcactc acctcgcttg tttctgctaa tgcttcaatc acactacctc
     2641 ggatgacgac accttttaat tttccatcct ctacaactgc aagtggagtc ggtgagtcat
     2701 aaataatatt gaaaatatca cttaccacca tgtctttccc aatcaccgtc acattttcat
     2761 ctaggaagtc gatcaatgga cgtttttgct tcctagcttc taacgcttga tctgcagtga
     2821 tgatcccttt caggtgtctc tttctgtcaa cagccatcag catgctgact tcttcattac
     2881 gcatccttgt caatgcaacg tttggtccat cagattcgat attcgtcgtc aatgcaggta
     2941 ccatgatgtt ttgagccgta agcactttgg aacgatcgac ttcttccaca aattcacgta
     3001 cataatcatt tgctggattg gtcaatattt cttcacctgt tccgatctgc atgatttcgc
     3061 cgtctttcat caaggcgatt ctgtccccaa tccgtaaagc ttcattcaaa tcatgggtaa
     3121 tgaaaataat cgttttttga acattggctt gcaggtccag taattcgtct tgcatttctc
     3181 gccggatcag cggatcaaga gccgagaacg cttcgtccat cagcaagatc tcaggatcat
     3241 ttgctaaagc tctggctagg ccaacccgtt gttgcatccc accagataac tgactaggat
     3301 attggtcttt aaatgaaagc aaactagaat tttctaatgc tttttctgct ttttcttgtc
     3361 gttcttcttt aggtacacca cggacttcta acccgtattc tgtattttcc aagatcgttc
     3421 gatgtggaaa cagaccgaag ttttggaaaa ccatattgat tttatgacga cggacttctc
     3481 gcaattcttc tttgcttaac ttcgatacat cttctccatc aatatagata ctgcccgaag
     3541 ttggctcaat cagacgattc aatagacgga tcaatgtgga tttcccactc ccggaaagcc
     3601 ccatgatcac gaagatttct ccttctttta cgtcaaaatt cacgtcatac acaccaaccg
     3661 ttgcaccggt tttctttaaa atctccgttt tcggctggtg atcattcatc atatctaaag
     3721 cggcttgtgt ctttttacca aatatctttg ttaaatgttt tacttctact tttaccaaac
     3781 aaattctcct tttttatgta aattcatttt tctttctgaa aaaagtgacc acactatgtt
     3841 aacatcatgt agtcactttg tcaaaaatga aagaaatttg cttgtccaaa ctgaaaaaaa
     3901 cttggctaaa tctttgtaaa taaaaggttt agccaagtta atattttttt aatatttcac
     3961 acaatcattc tgcaagctga tgctccgggt aaaaaaagtt tgtcatacgc tgtagtgtat
     4021 attcatttcc aataaaatga acgatgtccc ctgccgtaaa tttagcatag gggccaggtg
     4081 aaacaagcag ttcttcatta tgagaaattg cgataactgt ggcgccagtc gcctgccaga
     4141 tattcaactc gctaattgaa cggtccagat gcattgcttt ttctgataaa gataattcaa
     4201 acggaagtaa aggattcatc ttatgtattc gcttcgtttg attgaccagc tgatccaata
     4261 attgagaaaa atgattcagc tcatcttgtt gcttctccac acttgccatg atttctgctt
     4321 ttgtatcttg gatggattgt acatcttgat attgcaatag gaaatctttt gctttttcct
     4381 tcgaagcaac aaatgcccca cttccatgcc gtacttccat gattccaaga tctaccagca
     4441 cattgatagc cttgcgggta gtctcaggag aagcattgta cgtactggca agtgttgagc
     4501 gggcatggat cttctcaccc actttatatc ggttttcaac gatacgttcg gctaaatcaa
     4561 cagcaatctg ctggtagcgc gggcggacga tttttttctt tccagacatt ttctttcctc
     4621 tttctttttt tactttccct catcttacta tgctctagca aaataagcaa aaaaggggag
     4681 aatagcctct tgcaacgact gatcatcgct tgatctgttc cttttttact gggcaagctg
     4741 tttgacagtc atcacaattc ttaccggatt ttaaacgagt atagacaatt gcgcctgcac
     4801 ttccaaagat taacacactt aataagaatg tcgccatcct attcctctcc ttctaatgac
     4861 aatatagtat aaaactggtt cttggatttt ggctgacgga acaaaacata aacgagtcct
     4921 atcatcagta aaatagcaag atacgtccct aacgctacct gtccaccctc caataagtgt
     4981 cccaactgat aaatcacaaa actaactaaa taagctaaac cgcattgata gcctatggcg
     5041 atccaagtcc atttcttggc attcatttcc cgatggatag ccccaattgc cgcaaaacaa
     5101 ggcgcacaaa gtaaattgaa ggctaacaat gaataagctg ctgcgggagt atatgtgtgt
     5161 tgtagcactt gccagatttc tctgccattt tcggatactt ccgcatgacc aaacaaaata
     5221 ccaaatgtgc caataatatt ttctttagcg accaatcctg taatcgctgc gactgttcct
     5281 tgccagtttc cccaaccaag cggctgaaaa acaactgcca ggattcgccc gaaaaaggct
     5341 aagatacttt ggtcttcaga aacaggttga gcataaaaat tataagaaga gctgaaccag
     5401 atcacgatac tagaaacaaa gataatcgtt cctgcttttt taacaaaaga cttgctgcgg
     5461 tcataggttt gtcttaggac cgctgaccat tgaggaaaat gatatgcggg caattccatg
     5521 ataaaaggag ctgtatctcc agcaaacaaa cgtgtttttt tcaagctgat tccagagaaa
     5581 ataattgcag tcataccaag aaaataggca gaaggagcta cccaagattg atgaggaaaa
     5641 aaggcccctg ctactaaggc aataatcggc aactttgcag aacaaggcat gaaggttgtg
     5701 accatgatcg tcaaacgacg atcttgctcg ttttcaatcg tgcgactggc catgacgcct
     5761 ggcactccac atcctgtagc aatcagcata ggaatgaagg attttcctga aagaccgaat
     5821 ttcctgaaaa gtcgatccat gacaaaagcg atcctcgcca tatatccgca gtcttccaga
     5881 aagctcaagc ataaaaacaa tataatcaat tgcggtagaa atcctaacac tgctcccacg
     5941 cctgcgatga tgccgttcaa gatcagctcc tgcatccatc ccgctacttg ccattcagct
     6001 aacaatctgc caacatgttc tggtaaccat gtaccaaaca atgtgtcatt gatccaatct
     6061 gtccccattg ttcctactgt ttgaatagcc agataataga tcagccacat cacaaatgcg
     6121 aagatcggca aggctaacca acgattcgtt gcaatctgat cgattttgtc actcatcgat
     6181 aattggaaag aatcatttga cacaacacag agtgtacata gttgggtaat aaattcataa
     6241 cgttcattga ccagaatcgt ctcactatcg tcgtccaata gtttctctgt taactggatc
     6301 agttgctcga tctctttttg ctgagaaact gctatctcgt aatcctctct taccttctga
     6361 tctcgttcaa aatatttcat tgcataccag cgtttcttgc cgctttgttc tcctagtaca
     6421 tcttcaattt catgtaaagc tgcttctaaa cgtggatcat atgtacaggg aacaggaata
     6481 gaaggccgct tttttgcaga ctcctgaatc ttttcgatta gtttttcgat tccttgcttt
     6541 ttcaatacgc tgattggtaa aactggtaca ccaagaccat aggaaagctt ttctagattg
     6601 attttttttc cttctttttt gatgacatcc atcatattca gaccaatgat cataggaatg
     6661 tctaaatcca ataactgcgt agttaaataa agatttcgtt ctaaattggt agcatcaata
     6721 atgttgatga tcagttcagg ctgttcctga accaaataat ctcttgcaat cacttcttct
     6781 ggtgtataag gagacaaaga atagatgccg ggtaggtcct gaatatgaat gttcggattt
     6841 tttttcaacg atccttcttt tttttctact gttacgcccg gccaattccc tacatattga
     6901 ttcgaaccag tcaagagatt gaacaagcta gttttcccgc tgtttggatt tccgactaac
     6961 gcgatattca ttctttttcc tccctttgta tcaagattag ttcggcctct gactttcgta
     7021 acgtcagttc ataccctcgc actgtcagtt cgattggatc tcccagaggg gctagttttc
     7081 ggataactat ttttacattt cgcgtcaatc ccatatccat taaccgtcgc ttcactgcgc
     7141 cttgtccgtg gattgctacg acgatcccag tctctcctgc ttttaattga tcaagagaag
     7201 tccagctttc agaggaagca gtctttttat tgacaaagat ttgacctagt atttcccgac
     7261 ttactgccac tcgatgactt tttaccagta aaatggcatt gttgcctgat aaattgacga
     7321 cgacgatttt acttccggca acgatcccga tattttgcaa atggcgtttt gttttttctt
     7381 ctgtttcgat tcgttcgact tgataaacga aattctgctt tgcctgcgct aaggtctgca
     7441 tattgattcc tctcttgtga aataaatcat gtattaaaat cattcattat atgttctaat
     7501 tttcttacgt gttacgcaaa taaatgagaa tcattctcac atagaatact ctcggtaatt
     7561 gagaatgtca attatacttg tgatattatg agcgaatctt tagtattgat agataaaaat
     7621 gagaaaaaaa tatttcttca tagttttcca caattttttc tctattaaat ttgctatttt
     7681 cccacacgat acttgttttc agctcttttt tcaaaaacac aaaacttagt attccacagt
     7741 ttccacaaca caatatgtag tgtttgttgt ttgacttttt tactaagagt tttatactat
     7801 ctattataga taataagaat gtgttagagg aggcactcac catgaacgta aaaatctttt
     7861 ctaaaaataa ttgtatgcaa tgtaagatgg tcaaacggtt tttaacagaa aatcaaattg
     7921 cttttgaaga agtcaatatc gatgaacagc ctgaagcagt agactggttg aaagcacaag
     7981 gattccaaag tgttcctgtc attacttctg atgcagaaac agttatcgga ttccgtcctg
     8041 atcagttgcg taaacttgcc agctaacatg actaaaagaa tcaaaacgtg atacatggat
     8101 ttgaagggct tgagacagca ccagtttttg tcgcaaactt ccaaatcctt ttttaaagct
     8161 taaaaacaat gcaatgattt gcaatcaagc atcatcaaat acgaaatgaa ggttgggcgt
     8221 acgtatgagt cttaaaaatt tacaagatgt cagttatttc aagctgaata atgaaatcaa
     8281 tcggccagtc aatggtcaaa tccctttgaa taaggataaa gaagctttag ttgccttttt
     8341 tgaggaaaat gtcaaaccaa atacgatgac ttttctatct attatggaaa aaatcaatta
     8401 cttgatcgat caagaatata tagaagcaga attcattcaa ttgtattcac catcatttat
     8461 tgaaaagcta tatcaatttt tggacgagca acatttttct ttcaaatctt ttatggctgc
     8521 ttacaaattt tattctcaat atgcgttgaa aaccaacgac ggttccgctt atctggaaac
     8581 ttatgaagat cgagtagcct tcaatgcgct ttactttgcg aacggggatg aagaattagc
     8641 acttgctctt gcagacgaaa tgatccatca acgatatcag ccagctacac cttctttctt
     8701 gaatgctgga cgtaaacgtc ggggcgaatt ggtgtcttgt ttcttgactc aagtcactga
     8761 cgatatgaac tctatcgggc gctcaatcaa ctctgctttg caattgtccc gtattggtgg
     8821 cggcgtaggg attactttat caaatcttcg tgaagccggc gctcctatca aaggatatga
     8881 gggtgctgca agtggtgtcg ttccagtcat gaaattattc gaagatagtt tcagctattc
     8941 taatcaatta ggtcagcgtc aaggagctgg cgtggtttat ttgaacgttt tccatccaga
     9001 cattattgct ttcctttcag caaaaaaaga aaatgcagat gaaaagattc gcgtaaaaac
     9061 tctttctctt ggcgtgatcg tacctgataa attttatgaa ttgacacgta aaaacgaaga
     9121 tatgtattta tttagtccat atagtgtcga aaaagaatat ggtgtcccat tctcttatgt
     9181 aaatattaca gctgagtatg atcatctagt tgcaaatcca aacattcgca aacaaaaaat
     9241 caaagcgcgt gatcttgaaa atgaaatctc aaaattacaa caagaatctg gctatcctta
     9301 cgtaatcaat attgatacag cgaaccgcga gaatccaatc gatggcaaga tcatcatgag
     9361 taatttatgt tcagagattt tacaagttca aacgccttct gtgattaacg gcaaacagga
     9421 atatgaagta ttaggaacgg atatctcatg taacttaggc tcgacaaata tcgtgaattt
     9481 gatgcaaagc cctaactttg gtcaatctgt gcgtacgatg actcgtgcat tgacatttat
     9541 tacagatgca tctgatatcg atgtcgtacc accgatccaa aacggaaata agctgaatca
     9601 tacgattgga ttaggtgcca tgggcttaca cacttatctt gcaaaagaac aaatggaata
     9661 tggctcagag gattcattag actttacaaa tatttacttc atgctgttga attattggac
     9721 acttgttgaa agcaacaata tcgctcgtga aagaaaacaa gttttccata attttgaaaa
     9781 atcagcttat gctgacggca cttatttcga aaaatacgta actggagaat tccagccaaa
     9841 atcagaaaaa gtcaaagcat tgtttgaagg catttttatc ccaactgctg aagattggaa
     9901 tgctttgaaa caagcagtaa tgaaagatgg actgtatcat caaaatcgct tggcagtagc
     9961 accaaatggc tcaatttcat atatcaacga tacgagtgca agtattcatc ctatcactcg
    10021 catgatcgaa gaacgccaag agaagaaaat cggtaaaatt tattacccag cgccttatct
    10081 atccaatgag acgattccgt attacacttc tgcttatgat atggacatgc gtaaagtaat
    10141 cgatgtttat gcaatggcac aacaacacgt cgatcaaggc atgagtttga ctttattcat
    10201 gcgttctgaa atcccagaag gcatttatga atggaagaca acaacgaagc aaacgacacg
    10261 tgatttgaat attttaagac attacgcttt ccacaaagga atcaaatcca tttattacgt
    10321 acgtaccttt actgatgatg cagaagaaat cggaagcaat caatgtgaaa gctgcgtgat
    10381 ctaatatcat agatagattc gcttattcat attttaatta aaggagcatt ctcatgtcag
    10441 aaacatatta tgaagcaata aactggaatg aaattgaaga tatcattgat aaatccactt
    10501 gggagaagct gacagagcaa ttttggctag atacacgtat cccactttca aatgacctag
    10561 atgattggcg tacattgtct caattggaaa aagatacagt aggacatgta tttggcggtt
    10621 tgacactgct agatactgtc caatctgaaa gtggcatgga tcagttgcgt aaagatgtcc
    10681 gcactcccca tgaagaagcc gttttgaaca atattcaatt catggaatcc gttcatgcca
    10741 aaagttattc ttctattttt agtacactga atacgaaaaa agaaatcgaa gaaatttttg
    10801 actggacaaa tacgaatcct tatctgcaaa aaaaagctga gcgtatcaat gaaatctaca
    10861 aacacggtac accgctagaa aagaaaattg ccagcgtctt tttagaaaca ttcttgttct
    10921 attcaggttt ttatactcct ctttattatt taggaaacaa caaattggct aacgtagcag
    10981 agatcatcaa actaatcatc cgagatgaat ctgtacatgg aacctacatc ggttacaaat
    11041 tccaattagg attcaacgag ttacctgaag cagaacaaga aacgttaaaa gactggatgt
    11101 acaatcttct ttatgagctt tacgaaaacg aagaacgtta tacagaagaa ttatacgatc
    11161 caatcggctg gacagaagaa gtcaagacat tcctacgcta taatgccaac aaagcattga
    11221 tgaacctagg gatggatcca ctctttccag atactgccaa tgatgtcaac ccaatcgtaa
    11281 tgaacgggat ttcaacaggc acaagcaacc atgacttctt ctcacaagtc ggaaacggct
    11341 atttgttagg tcatgtagaa gcgatgaaag acgacgatta tttgattgga ttggaataag
    11401 gatttatttt aattcaaaaa tttttcgaaa aataaaaaga gtttagcgta cagatatttt
    11461 aaatgtacac tgaactcttt tttatgctta ttcacttgtt tcttccagca cttttggact
    11521 gtctaacttt tccgaaacat gttccattct tctcaataaa tagattttcc ggatatttaa
    11581 tattatcgtc ttaatcactg catagacagg tatgcaaatc agcatgccta atattcctgc
    11641 gacacttcct gctccaatca ataccaagat gatcgtcaaa ggatggatat tcattgattt
    11701 cccaaataac aacggcttga tcaaatttcc atctaattgt tggatcacta aaatcgacaa
    11761 cgccatatac aaagcttgaa atggagagat aaacaaaccg acaatgattg ctggtaccgc
    11821 tccaataaaa gggccgacat acgggataat attcgtgatt ccgcaaatga ccgcaagcaa
    11881 caagctataa ggctgatcga atattctcat tgccaaaaag ctcattacac caataatcaa
    11941 agcatctaag atcgtactgc tgatataggc agataatgtt tcgtttaatt cttttaccgt
    12001 ttggcgtaac tctttacgaa tcgctttcgg aaagaagtga gacaacgcat caataaattt
    12061 gtgcccatcc ttgaacataa atagcaaaat aaatggaacg gtaaataaca aaataaaaaa
    12121 ttgcatcata aatgaaatga tttttgagac acttgcagat aaactgacta atatgatatt
    12181 gaacaaatta ctaagagata aatttgctga tgctaattgt tcttccaaat tgaaattttt
    12241 aaactcttcc cgctgcgcca attcatttaa ccatttgctt gtttcatctg catacattgg
    12301 caagctctgg gtcaattgaa ttgtctgttc cattaactga ggcactacat tcatgacagt
    12361 taaaacaact aaaatgatta cgaccaaaaa cgtcaataaa aaaccgaaca ctctcgggat
    12421 ccgatgcttt tcgagaaaaa caacgattgg atcgaacata taatagataa aaccagcaac
    12481 aagcaatggt aaaaaaactg ccgaaagcat tttcaatatt ggactaaaaa tattcggcat
    12541 ttgtaaaata aagtacacgc cgatgatcgt gagtactagc tccactgtcc agaaaaataa
    12601 ttttgattgt ttaaaacgtg attgcataca tctcctccaa ggtcctttga tacttctatt
    12661 ctatcattta acaaagagtt acgcgctgag attttcattt gcttaaaaaa aacaaaaaaa
    12721 ataaacatat tttaatgagt agacgagtac ttaaacaaaa acacggtttg gagtagtgac
    12781 aaaaattata ctgcagtccc tttttccaaa taaacggtgt tcaaaagctt gcatcttcgt
    12841 aataagccca gccaaacgaa aaatataagg aactattttt atttggctgg gcttattact
    12901 tgatgtagga catctatttt acaacctcag atcatttatt gcgcaatact gactaaatgc
    12961 aactttgtga tatttttgtg tacggacaac tcctcgatca catcggcata agtgagccct
    13021 tttggaagat caatcgtata gatatttttg tacactcggt agtcatcaac gaattttacg
    13081 tcaaaattca catcttcgat tacgatattt ttctcttcaa aataatcggt aagaaaatct
    13141 ttggtttctt ttctatggat aaattggatt tctaactttt tagtcgtagg tacatggatc
    13201 acgcgtttca ctactgtgag tgcaatagca atcccgataa aactgatgat cgcaatggaa
    13261 tagtaaccca tgccgatggc gatccctaat ccagcaattg cccataaaga agcggcagtc
    13321 gtaagtcccg tcaccgattg tttcgtgacg atgatcgttc ccgcgcctaa gaaaccgaca
    13381 ccactcacca cttgagcaat cagccttgct tcatctgaac gaatcgttcc agcaagctgt
    13441 ggatactcta aagcatctct taatgcacca ctagcgatct caacttggat caatgcaatg
    13501 atacatgctc ccatacaaac taaaatatgc gtacgcatac ctgctggacg atttttatat
    13561 tgtcgttcaa aaccgattgc tccgccaaat agcatagcga tccctaaacg taaaataatc
    13621 tctggaattg tcaataccat atcattcatc taaacaccct ccagtccaat atatcatgtc
    13681 tgaaaggagc agacaaaaaa agtgcaattt ttacctctta cgaaaaaagg actgtgacaa
    13741 aatttttgtc atagtccttt ttatgaataa acggtgttca atcagctgac cctcggtatt
    13801 actcaacaaa cggcaaaata tggggagcta tttttccttt gtcttctcta atactcaggt
    13861 caaaacgctg atttcacaac ctctttagcc tactattgaa aattacaact tacttgctgc
    13921 ttcttcatcg atgatcacaa caacattgtc gtgattttgc aatacacttg ctggcaaatg
    13981 attcgttaca ggtccttcaa tcatcccttt gattgcatca gctttgcttt cgccaaatgc
    14041 catcaagacg atttctttac ttttcataat tgaaccgatt cccattgaaa cagcttttgt
    14101 tggtacatct tctgctttat cgaagaatcg tttgtttgca ttgattgttg attctgttaa
    14161 gtcaactact gaagtcgttc cgtcaaatga agcaccaggt tcattgaaac cgatatgtcc
    14221 attttgcccg attcctaaga tttggatatc gattggatgt gcatcgatga tttgatcata
    14281 ctttttgcat gctgcttcca gatcttctgc ttttccatct ggtacaaacg tttctttgaa
    14341 tggtttttta tcaaataatt ggacatccat gaaatgacgg tagctttgtt catctgaacc
    14401 gcctagtcca acgtactcat ctaagtttac agaagtcatt tcactgaaat ccaaatcgct
    14461 tgaagtcatt tctttatata aagtaactgg tgtgcttcct gttgccaatc ctaatacttt
    14521 tgcacctttg ttcatacctt ctttgatgat ttcaaatgct ttttttccgc cttcttcagc
    14581 gttttttaca cgaatgattt ccatagtgat tgctccttta tattttggta tagcccaatt
    14641 atagcattct ttttttcttt ttacaataat tagtatagac caaattgcat ttttttcatt
    14701 ttcttgacaa taaataggaa tccgtgttag gtttcataag atggttcaat caaacaatcc
    14761 atgttgcatg aaaaccaagc aagtgattca ctgtggacgc tttatgtctg cagttttttt
    14821 atgaaaaagg agttattatg agtatactga cattaaacaa cattacccag caatttggcg
    14881 acaaaatcct ctatgaaggt gtcgatctgc aattaaatgc tggcgaacat cttgggctga
    14941 ttggacaaaa cggagccggt aaaagtacat tgattaaaat catcactggt gagatcttac
    15001 cagatgacgg acagatccat tggcaaaaaa atattcacaa aggttacctc gatcaatatg
    15061 tggaagtcga tgaaacatta acaatcgaag aatttctaaa aactgcgtac gcaaaagaat
    15121 acgaaaagga agaaaaaatt gcttttcttt accaaacata tagcgagacc ttcgatgatt
    15181 ccttgctgga aaaagctggg aaactccaaa cggaattaga ccaaggagtg ttttatcaaa
    15241 tcgacacctt agttgctgag atgagcagcg ggttggggat cgatgtctta ggtttagatt
    15301 cactgttgaa aaatctaagt ggtggacaac gttcaaaggt gattttagca aaactacttt
    15361 tagaaaaccc agatgttctg atcttagatg aaccaaccaa tcacttagat gatcagcata
    15421 tccaatggct gacgcagttt cttcaggatt ttgacggtgc gtatatcgtg atttcccatg
    15481 accaagaatt tcttgatcag atcaccaccc atatcgcaga catcgagttt ggaaagatta
    15541 ctaagtatac tggccatcta aaacaagcat tgaaacaaaa ggaacaaaat cgcgaaagct
    15601 atttgcgcca atatcatgct caacaaaaac aaatcgaaaa gaccgaagct tatattcgaa
    15661 aatacaaagc cggttcccga tcaacgatgg caaaaagccg acaaaaacaa ttagataaaa
    15721 tcgaacggct gacgcctcca gcttcagcaa gtaaagctgt ctttgatttt ccattctcgc
    15781 ctattgtcac gacattagca gtggaaacaa ctgatttagt catcggatat gatcgaccgc
    15841 tacttgcacc catcaatttg accatccgct ttggtgaaaa tattgccatt cgaggcttca
    15901 acgggattgg aaaatctact ttattaaaaa cactgatcgg tgagattgaa aaaataagtg
    15961 gcgatttcca cttcccagat aacacaaaaa tcaattactt ttcacaagat ctgtactggg
    16021 aaaatccatt agaaacacca ctacaatatt taagcagtca atttccaaaa gcgactatca
    16081 aagaattacg tcgccaacta gctaaagcag ggttagttaa tcaattagca agtgaacctc
    16141 tcactacatt aagtggtggg gaacaaacta aggtgaaact tgcacagttg acaatgaacc
    16201 aaggtaattt actgattctc gatgaaccga cgaaccatat cgatcaagaa acgaaagaaa
    16261 gcttacaaga cgggatacag aaatttccag gaacagtaat catcgtctct catgaacaag
    16321 aattttatca agatcttgtg caacgagtca ttgagattga accaaaataa acgtttgatt
    16381 gaatagttga aaaaaaggag ctgtgacaaa agttttgtca caactccttc ttctgaataa
    16441 atggtggcca attagctgac cttcggtatt agctcaaaaa acggcaaaat atgaggagct
    16501 atttttattt ggctattctt attacttgat gcagaacagc ttttcacaac ctcttttcgt
    16561 ctatttttta ttttcgagat ttctctttcc tttaaaaaca actgtttgaa agaagaacgc
    16621 acatccttga aaaacaagcc cgctaaccgt tacacctaac cagccatact gctgccacat
    16681 tgtcgtccct agcaatgaac cagccgcacc tccaataaag tacataaaca taaatacagt
    16741 attatttcgg ttgcttgctg cttcacctag cccttgtact cgtgtttgat tggcgacttg
    16801 accaaattga ttcccgacgt ctaataaaat aatagctaaa agcaaaatgg ccaaatggct
    16861 tccgccaacc aataaagcaa taaagcttat tgtttgcata acaagtccca tatacacgat
    16921 ttttctttca gaataagaat ctgccagtcg tcccacgata ggtgcagcaa ctgctccaga
    16981 caaaccaaaa atcgctaata tccctgcttc gaatgttccc caattgtagg cgggactgct
    17041 gatataaaaa atcaacgtag accaaaagat cgaaaatgtt ccaaacatga aaaatccact
    17101 gactgccgct tctcttaata gacgttgaga agcaactaaa tgcggcaaac ttttaagtga
    17161 atcaaggtat gaaacagctg tactactttg ctggacagaa cgcggcattt tccagtaaag
    17221 cagaagtgta agaattccga ctgcacataa agcaaataaa tagatcatcc gccaagaaaa
    17281 aagactggca acaataccag ataccgtccg agaaagcaaa atccccacga gtaaaccgct
    17341 aagcaatgta cccatgacct tccctcttgc ttttggtccc gctagtaccg caccgtaagg
    17401 aatgatgatt tgcggcacga tcgaaagtaa tcctattaaa aaagaagcgc atgcaaataa
    17461 cgtaaacgaa ggcgcaaaaa aagcggccaa cagagagacg gcagataacg ctgccattcg
    17521 aatgatcaat tttggacgat ccaccacgtc ccctaaagga actaaaaaca gtaaccctaa
    17581 cgcatagcct aattgggtca acatcgtcaa tatcccgact gcacttgtgc taacagataa
    17641 actagctgct acttttgttc caatcggctg gatgtaatac atattggcaa caacaacgcc
    17701 acaagtgatt gataatagta aaataagcga agttgttaaa ggtttttttt cattactctt
    17761 cattgatttc ccccatttcc tatcttagtt tacaacttat ggaaaagatt ggaaatagaa
    17821 aaatacaact attcgccaca cttattattt gtgcttttgt tttccttgtt ttttcttgcg
    17881 atctttataa atgaagacaa ctaaccctac tagcacagta acacttgcaa ccaacgtgac
    17941 tgcagttttg ttatatccgt gaaacagagc ttctccacca cacgcataca acacagacga
    18001 agggatgact ccaatcacga tcgacaatat gatttgctgt agaggcaatt tcatcgcatc
    18061 tgcagcaaaa ttgaccatag aagatggaat aaccggtatc atatagccta acattacacc
    18121 tatttttgga tgtttcattt gtcggattgc ttgcacccaa tgattcgttt cggttttctt
    18181 atctaaaaat tttaagcgat gcataaggaa aattgcagtg atgttaccaa gagcattacc
    18241 caaaatgttg atgatcgcac ctgtcagtgg tccaaagctc aaaccagcaa tgactccaac
    18301 tactgaagta ggaatacctg gaacagcaca catcaccgct gtcaatacga tcaagagtat
    18361 cgatgcaaaa gtcccatgcg aacgaatatg tgcgagtagc tctgccttac tcgctctcgg
    18421 atttaaaaat aattggatgt ctgtctgata ttctaaatac aaccgatagc ccactaccag
    18481 tatcagaatg attcccaacg agatcaatat caatcggatt attttctttt tcatatttcc
    18541 tcctgaaaaa aatctatatt cattaaataa tagtttaaca taaaaacaaa aaagcaccga
    18601 aatccaacat aggatctcag tgcttttaaa tagtaaaaat tatttaacag ttacagatgc
    18661 gccaacttct tcaagtttag ctttcaattc ttcagcttct tctttagata cgccttcttt
    18721 aactggtgca ggagcgccat caactacagc tttagcttct ttcaagccta aaccagttgc
    18781 ttcacgaact gctttgataa ctttaacttt ttggtctcca gctgcagtta attctacagt
    18841 gaattctgtt tgttcttcag cagcagcagc gccaccagca gcagctacag ctacaggagc
    18901 agcagcagat acgccaaatt cttcttcaat agctttaact aggtcgttaa gttctaggat
    18961 agttgcttct tttaactcag caacgatgtt ttcaatgttc aatgccatta taatttcctc
    19021 cgtttaattg ttctttttta tttaatgctt tatgcggcaa ttaagctgca tctccatctt
    19081 ctttgtctgc cactgctttg acagcgtaag ccacgttgcg gactggcgct tgtagtacag
    19141 atagcaacat agatagaagt ccttcgcgat ttggcaattt tgccaatgct gtgatttctt
    19201 ccaatgaaga gactttacct tcgatgatac cgcctttgat ttctaatgct tttgcttctt
    19261 tagcaaattc gtccatgatt ttcgctggtg cgactacatc ttcgttactg aatgcaacag
    19321 ctgtaggtcc agtgaatact tcgtccatgc cttcaaggcc agctttttta gctgcacgtg
    19381 aaagaataga atttttgata actttcattt ctacgttagc atcacgaagt tgtttacgta
    19441 aacgtgttac ttcatcaact gttaatccgc ggtagtcaac gactactact gatgcagctg
    19501 ctgtaaattt ttcgcttact tcatcaacga ttgctgcttt ttttgcgata attgcttcac
    19561 tcatttttga gtttcacctc ctgaattttg atggaagagt tttttgcact gaaaaggcaa
    19621 gtcactttta tagttacttt gctcaaccag tattattcat cacctattca cttcgttcat
    19681 agtgattcta ctcgctttgc tcgaaaggaa actcctttcg ctttgctcaa ccagtattgt
    19741 tcatcatctc ttcacttcgt tcatagtgat tcacaataaa aaactctatg ccaccgcaga
    19801 catagaggga ctattgaata tattcaataa acgtcctcgg taggaaatta aggcttgatg
    19861 ccacctactg tcttcggtac aagttaatta ttaacctcaa ctagcttagc atgctaaact
    19921 agttgagtca agaattattt ttcagttgga tcaatcaaca ttctaatatt taattagaaa
    19981 gaagcttggt caacatggat accaggtcca aatgttgttg tgatcgttac atttttcatg
    20041 tattgacctt tagctgctga tggtttagct tttagcaata catcatggat tgctgcaaag
    20101 ttttcaacta atttttcatc gtcaaatgaa acttttccga ttggtacatg gatatttcca
    20161 gctttgtcca cacggtaagt tactttacca gctttcactt cgtttactgc ttttgttacg
    20221 tccattgtaa ctgtaccagt tttagggttt ggcattaagc cttttggtcc taatactcgt
    20281 cctagacgac ctacagtagc catcatatca ggtgttgcaa caactacatc aaagtcaaac
    20341 catccgtttt ggattttttg aaccatgtcg tcgtcaccta cgaaatctgc accagctgct
    20401 tcagcttctt ttgctttgtc tcctttagca aaaactaaaa cagtttgtgt tttaccagtt
    20461 ccgtttggta atacaacagc accacggatt tgttggtctg cttttttagg gtctacgttc
    20521 aaacggtatg caacttcaac tgttgcatca aattttgcaa tgtttgtttc ttttgctaaa
    20581 gctactgctt cggcaacagg ataaacttta cttgaatcaa ctttttttaa tgcatcttgc
    20641 atttttttgc tctttttagc cattttggtt cctccttgat tgtggttata acggttgtac
    20701 ctcccacgtg ttctatatct ttcaatatag agccaactga gaagctgctg tcttagggtg
    20761 tatagattat tctacagtga tccccatgct tcgtgcagtt ccttcaacca tgcgcattgc
    20821 tgcttcaacg ttagctgcgt ttaggtcttc catttttaat tcagcgattt ctcttacttg
    20881 atcgctagaa actttcgcaa ctttattttt gtttggttcg cctgaacctt tttcgatttt
    20941 agctgctttt ttcaatagaa cagcagctgg cggtgttttt gtaacgaatg tgaatgaacg
    21001 gtcttcgtat acagaaataa caaccggaat gatcaaacct gcttgatcag ctgtacgagc
    21061 gttgaattct tttgtgaatc ccatgatatt gatacccgct tgacctagtg caggacctac
    21121 tggtggagct ggagttgctt tacctgcagg aatttgcaat ttaacgattt tttctacttt
    21181 ctttgccacg agacatacct ccttaagtcc gtgatgtggt taattggaga tgcatatttc
    21241 tcctcccacg ttttatctga tctgaaaaaa tccagacaaa taatgtgcaa aacaatgcac
    21301 actggtacag tatagcaaat ttatttgttt tttcaagatt tttttctgct atttttacat
    21361 tttgcttgaa taaacgctaa atttattcta aaaaaacaac aatttttcgt tctcatttct
    21421 tcttttatgt tacacttgcg ctagcatggt tcaacagatg aaagcaaaca ataacacggt
    21481 tttttagacc cttgtacaaa gattcctaca taacaaaatg aggtggagaa aatgaaagat
    21541 ttaaggtata gaagtgtctt tgatattatt ggacctgtga tgattggtcc aagtagctct
    21601 cacactgccg gagctgctag aatcgggaaa atcatacgta gtatatttgg cgaacagcct
    21661 gattctgtag atatttatct atatgaatca tttgcaaaaa cctatcgggg gcacggaaca
    21721 gatattgcat tagttggcgg cctgttaggg atggaacccg atgatgaacg tttagctgat
    21781 tctttgaaat tggcatatga agccggtatg gaagtcttgt ttgttcctaa gagtgaaaaa
    21841 gccgaacatc caaactctgt aaagatgctt ctaaaaaaag gaacacgaaa gctttcagtc
    21901 actggcattt cgatcggcgg tggcaatatc cagatttctg aattgaatgg attcaaaatt
    21961 tctttaagca tgggtactcc taccttgatc gttgtccacc aagatgttcc aggtatgatt
    22021 gctaaagtga ctagtatact ttctgagacc aatacgaata tcggcactat gacagtgaca
    22081 cgggaatcaa aaggtgaaaa tgccattatg ataattgaag tagatgatcc tcaagtagaa
    22141 gaaacggtaa aaagactcaa acagctgcca aatatcgaca gcgtaaatta tttcgattaa
    22201 ggagctaaaa aaatgttttt atcgattgaa gaattagtag atcaagcaaa agcttatcca
    22261 agtgtagcag aattgatgat tgcagttgaa atggaaatga gtggccgttc ccgtagtcac
    22321 atcatcgaga tcatggaaaa aaacctctca gtaatggaac aatcaatcaa cgagggaaaa
    22381 gaaggtgtta cctccgtcac aggaattacc ggtggtgatg caaaaaaatt agatgattat
    22441 ttaaatgagg cagacttttt aagtggagaa acaatcttaa aagctgtacg caatgctgta
    22501 gctgttaatg aagtcaatgc aaaaatgggg ttgatttgtg ctacaccgac tgccggaagc
    22561 gcgggtgttg ttcctggtgt gctgatggct gtccgagatc ggcttgattt gacacatgaa
    22621 cagcaattgg aattcttatt taccgctggc gctttcgggt tagtcatcgc aaacaacgct
    22681 tctatcagtg gtgcagaagg gggatgtcaa gcagaagttg gttctgcaag tgcaatggca
    22741 gcagcagctt tggtttgtgc aaaaaatggg actcctcatc aagcagcaca agcagtagcc
    22801 attacattga aaaatatgat gggactgatt tgcgatcccg ttgcaggctt agttgaagtc
    22861 ccgtgtgtca aacgtaatgc tttaggctct tctcaagctt ttatctctgc tgatatggca
    22921 ttagcgggta tcgaaagtgt cattccgccg gatgaagtcg ttgctgcaat gtaccaagtc
    22981 ggacgtcaaa tgccgtcgat tttcaaagaa acagcagaag gtggcttagc tgttacgcca
    23041 actgctaaac gacttgcgga agagatttta aataagaggg cgtgataaag ctccttaatt
    23101 tcgagtatta agagattagt tatcttcata acaaaaaacg aagagaggac taattgccct
    23161 tctcttcgtt ttttattaat ccatcaattt cgtatttttt acttcatcga tcgtttttgt
    23221 tcctgcaagt tgcatcgtga tcgacaattc tttgttcagg tgttcaaaaa cagatgttac
    23281 tccttctgcc ccacctagat tcaacccata aatcactggt cggccaatcg caactaagtc
    23341 agcaccactt gccagagctt tgaagacatg ctctcctcga cggacaccag agtcgaaaat
    23401 cactggtaca cgtttgttga ctacttcggc aatttttggc aatacttcaa aggacgcagg
    23461 acctccatct agttgacgtc caccatgatt ggatacccag ataccatctg cccctgcaga
    23521 gatagcgatt actgcatctt ctggcgattg tattcctttt acgataactg gaagatgggt
    23581 gatttctttg attgttttga tgtcatctgg tgtcaagcct tgttttgcag ctgcataaat
    23641 ttctgcgatt ccttttcctt cgccattacc acttgcactt tgttcgctat acgcagctaa
    23701 gttaggcatt ggtaatggaa attggaattg attgatgacg tcttcttcac ggtaaccacc
    23761 gagcgttgaa tctgccgtta aaatgatagc tttagcccct gcttctactg ctttgttcaa
    23821 aatgaactca ttgaatctgt cgtctttact catatataat tggaaaaact gcggtgcgcc
    23881 tggcgctgca tcggctgcgt ctttgattgt cgtatttgca taagtgctga tggaaaagat
    23941 tgatccagca gcagctactc ctttagctgt atcagcttcc cctttttcat gtgccaatcc
    24001 ttgggccgct gatggcgctt ggataatcgg tgtatctaat tcaattccga aaacagatgt
    24061 atgcaaatcg gctgagtcga ttccgcgtaa tatccgtggc atgattttct ttgtattaaa
    24121 agaagtcgta ttttctttca tcgtccactc atcttcagaa ccaccgcgaa tatagccaaa
    24181 agctcctgct tccatgcgct cttttacacg accttctagt taggcaatgt ttacaatatc
    24241 gattggatgt tcttcattac ttgtatgata agtcatagta gatccttctt tcatcttctt
    24301 gattacatat aaatcatact aaacttacaa aaagtaagca aacatttact ctcagatact
    24361 tttatcgcta ttctttccaa ataaatcgac ttacaagctg atctacttct tggttttcat
    24421 gaagcatact atgagaggca ttttcacctg tcactacttt ttcctctatt tcatttccgc
    24481 gttgcttcaa caacgcatag accgctaatg cactgtttaa cggtactgtt ccatcactca
    24541 agtctgtttc atctagttga ccagcaatca agaaaaatcg agtggttata ggaatattac
    24601 cgatcatttg ctggtaatct tgatagcggt tagaaacgac agcaggcccc tttgctaact
    24661 catctgtcag agactgcgca ctatcatcga caaaatcatt aaaaggcgcg ccaattgctg
    24721 ttaatgtctg aacttgtggc aatgacgtat cctgtccata cgttcctaaa tagcgtagtg
    24781 cactaactcc tcccatggaa tgccccacta tgttgacttt atctatgccg tagttttctt
    24841 tcagatacaa aagacaagtt ttgatccatt ctgcttgatt ccactcgtta tttttattat
    24901 cttcaaacaa tacttggacc atcggattgt ctgcttttcc agacaaatct cctgttgctt
    24961 gtatttgtcc atctgttcct actgttaaaa ccaattcttt tactgcttgc tgattttttt
    25021 ccaaacgccg caacatccct ttaaaggaat tttctgtccc gctgtagccg tggaaaaaaa
    25081 gtgttggaat agtggttttt tctatcactt tactggattc tgccgttttt tctttatcag
    25141 aaacttcagt agtatttatc ttcgtcgtct ggcaaccact taacgcaaac aaactgataa
    25201 ggactaaagc tgctgcagtt gcacgtttgc ataaattttt gattggttct gtcatacacc
    25261 tcttcctttc catatagaaa aaagaaagac ttgaaaggcc aatcatatga tcgacatttc
    25321 aagtctcttt attaatcgat tttatctact tgatcgaaat caagttctgt actggtttca
    25381 cgtccaaaca tatcgatgtt gactttcaat ttttcttttt ctccgtcaac ttctgttact
    25441 tgtccttcta atccagcaaa tgcgccttcg atgattttca cgacttcgcc aacttctact
    25501 tctagatcat tttgtcgcag gctcataccg atagaacgca agatatgatt gacttcttct
    25561 ggaagtaatg gtgctggttt gcttccggct ccgtgtgaac caacgaatcc tgtcacacca
    25621 ggtgtgttgc ggacaacata ccatgaagcg tctgtcatga tcatttcaac aagtacgtat
    25681 cctgggaatg ttttatgaac gatttctttt tctttcccat ttttgacttc agtttctttt
    25741 tcttcaggaa caacgacacg gaaaataaag tcggccattt tcatactttg cgcacgagat
    25801 tcgatatttg cttttacttt gttttcatat cctgaatatg tatgcaagac ataccagttt
    25861 ttttcaaaag attccattga aggctcctca tttcattgta aaataaaaaa acctcagtac
    25921 acccgaagtt tttctttcgc ttcattatag cactaaaagt acgttgtggc tagcgagtta
    25981 ttttaaaatc cagccaaata agctttggat ggctgtatcc attacgtaaa acatcaatcc
    26041 gaaaataata gaagtttcga tgactactaa cgtatcttta cggagttgtt ttctgcttgg
    26101 ccaagttact tgtttgattt catcttttac gctttttaga aatttcatga gacaacctcc
    26161 cttgctactg tttatttcgt ttctttatgt aatgtatatt gattacaata tttacaaaac
    26221 ttattgattt ccaaacgttc gccgcttttt ccttcactga ctgattttgt gtagttgcga
    26281 gacccacaaa cggaacaagc gagtgctgct tttttctttg ccatttactt ctgccccctt
    26341 gctgagaatc agacttatac ttcataacag tatcacgtaa ctttttactt gtcaatttgt
    26401 gaaataaact atctattgtt ttggataaaa ttatgatatg attaatttga aattaagcta
    26461 aaatgagaca attggaggta tagcgatgct attaaaaaca cttgtatata agaaaaaaga
    26521 tctgacaacc gttacagaga ctagtacatt agaagaagca ttaaaaattt tagaaacttc
    26581 cggataccgt tgtgtgccga tcttagattc ttcaggtaat attttccgtg gaaacattta
    26641 caaaatgcat atctatcgtc ataaagcaaa tggcggtgat atgacgcttc ctgtcactca
    26701 tcttttgaaa aacgctacaa agttcattta cctggatact tctttcttca aagtattctt
    26761 tacgatcaaa gagcttcctt atattgctgt ccttgacagt gaaaatcatt tttatggtat
    26821 tttgacacat agtacgttgc ttaatatgct ctctcaatct tggagcgtag aacaaggaag
    26881 ctatgtactg acaattgctt ccacaggaaa gcaaggagat ttggctgcta ttgcaaaaat
    26941 catttcaaaa cacagcagca ttgccagctg tattactctt gatatcgaaa aagacgaata
    27001 cattcgacgt acattgatca cgctaccggc taacactgaa gcgaaagtat gtgaaacgat
    27061 cgtcaaccac ttagagaaaa agaattataa agtggtagaa attgaaaatt tgcaaaacca
    27121 agaagcataa tcaaaaagcg aaaaaagaaa acagctaatc tgctttcttt tttcgctttt
    27181 tataattaga cacgcttttc aaaatcaagc gcgcgatcta aataatacat caatggtgct
    27241 gaaacagcca tgacgataaa ctcacttaat gcaagcgtgc cataggttgt ccaaaatgct
    27301 tggccacctg ttggaactaa catataagca atcagtccca tgctgatggt aaagaagatc
    27361 gtgttcaata ctaaacgcat ctttacattt ttcactttgt tttgcaataa agctgtcagg
    27421 cctaaagaaa gcaatgtttg gccgccacca aaaaccacat ccatgatacc aaaaccaaag
    27481 aaggcattat agacgatgac cccgcctaaa actccccaaa gcaatttacg gttaaagaca
    27541 actaaatgat tcaaactttc tgaaatacga aactggatcg gccctgagga tacaggcgca
    27601 actaaaatag ttaacgctag ataaagagcc atgatgatgc catttacaac gattacggat
    27661 aaccgttgtc tgcttttcgt tttttgcatt ttctctcctc ttttctccta gtttttttaa
    27721 cgcgggatgg ttgatgaacc gcggttatgt gaaacaaaca ataattataa cagacttttc
    27781 atttatttca atccgtctta tctgctttta aaaaagcaaa agcttaaaca atgtaaacgt
    27841 ttgctaaaaa tcgtgggata tggtataaag taagtgtaag gggtcataag aattcccagt
    27901 aaagcagacg aagtaggatc tgcaagaaca gaattgttgt gaaacttaaa taaataaaaa
    27961 aggatgtggg aatttgagca acctcttact agagaacggt ctagcaagca gctgaagatc
    28021 taagtggaca tgccgaacct tggaacttct ccttacgtaa gtaatggatc atgcgcttgc
    28081 tagg
//
LOCUS       DANKHL010000009        27594 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000009 DANKHL010000000
VERSION     DANKHL010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.011"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            78..725
                     /locus_tag="PW101_000297"
     CDS             78..725
                     /locus_tag="PW101_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL2512729.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            809..1753
                     /locus_tag="PW101_000298"
     CDS             809..1753
                     /locus_tag="PW101_000298"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HDL2512730.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1888..3339
                     /locus_tag="PW101_000299"
     CDS             1888..3339
                     /locus_tag="PW101_000299"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HDL2512731.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3360..4196
                     /locus_tag="PW101_000300"
     CDS             3360..4196
                     /locus_tag="PW101_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL2512732.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4196..4855
                     /locus_tag="PW101_000301"
     CDS             4196..4855
                     /locus_tag="PW101_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2512733.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            4981..5670
                     /locus_tag="PW101_000302"
     CDS             4981..5670
                     /locus_tag="PW101_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512734.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5676..6632
                     /locus_tag="PW101_000303"
     CDS             5676..6632
                     /locus_tag="PW101_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDL2512735.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6676..7188
                     /locus_tag="PW101_000304"
     CDS             6676..7188
                     /locus_tag="PW101_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2512736.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7444..7992)
                     /locus_tag="PW101_000305"
     CDS             complement(7444..7992)
                     /locus_tag="PW101_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2512737.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8081..9262)
                     /locus_tag="PW101_000306"
     CDS             complement(8081..9262)
                     /locus_tag="PW101_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDL2512738.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGLGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9498..11006)
                     /locus_tag="PW101_000307"
     CDS             complement(9498..11006)
                     /locus_tag="PW101_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDL2512739.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11165..11356
                     /locus_tag="PW101_000308"
     CDS             11165..11356
                     /locus_tag="PW101_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512740.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11559..12476
                     /locus_tag="PW101_000309"
     CDS             11559..12476
                     /locus_tag="PW101_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HDL2512741.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12469..13542
                     /locus_tag="PW101_000310"
     CDS             12469..13542
                     /locus_tag="PW101_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512742.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13690..14208
                     /locus_tag="PW101_000311"
     CDS             13690..14208
                     /locus_tag="PW101_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512743.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14189..14581
                     /locus_tag="PW101_000312"
     CDS             14189..14581
                     /locus_tag="PW101_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HDL2512744.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14657..15592
                     /locus_tag="PW101_000313"
     CDS             14657..15592
                     /locus_tag="PW101_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDL2512745.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15725..16504
                     /locus_tag="PW101_000314"
     CDS             15725..16504
                     /locus_tag="PW101_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL2512746.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16497..16814
                     /locus_tag="PW101_000315"
     CDS             16497..16814
                     /locus_tag="PW101_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL2512747.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            17058..18668
                     /locus_tag="PW101_000316"
     CDS             17058..18668
                     /locus_tag="PW101_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2512748.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18825..19673
                     /locus_tag="PW101_000317"
     CDS             18825..19673
                     /locus_tag="PW101_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2512749.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            19904..20383
                     /locus_tag="PW101_000318"
     CDS             19904..20383
                     /locus_tag="PW101_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512750.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            20416..22644
                     /locus_tag="PW101_000319"
     CDS             20416..22644
                     /locus_tag="PW101_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2512751.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            22773..24416
                     /locus_tag="PW101_000320"
     CDS             22773..24416
                     /locus_tag="PW101_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL2512752.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(24489..25034)
                     /locus_tag="PW101_000321"
     CDS             complement(24489..25034)
                     /locus_tag="PW101_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDL2512753.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(25057..25542)
                     /locus_tag="PW101_000322"
     CDS             complement(25057..25542)
                     /locus_tag="PW101_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2512754.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(25532..26344)
                     /locus_tag="PW101_000323"
     CDS             complement(25532..26344)
                     /locus_tag="PW101_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HDL2512755.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(26483..>27594)
                     /locus_tag="PW101_000324"
     CDS             complement(26483..>27594)
                     /locus_tag="PW101_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDL2512756.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         9040 a         5026 c         5522 g         8006 t
ORIGIN      
        1 ttttaaacat gacagaaaat tttttcttcg ttatacttgt aatagaatac tattttataa
       61 caaaggggat cgaatcaatg aaaaaaactg ttatttatac tagtttattt gcagctttgt
      121 ttttagctgc ttgtcaatca aataccaatc aaggcacatc tgggaatacg tcttccagta
      181 gcgaacagcg ctcttcacag gtatcggact cctcaacaaa agcaagcaca tcttcgtcta
      241 gccagacaaa aagttctagt gaagcaagta ctcagtcaga tatcaaagtg tctgcagctc
      301 gtgcgatcga attgttccaa gataaatacc cagatactgc aattactagt ttggaattag
      361 atagtgattg gggatcttac ttctacaaga tagaaggtgt cgatgatcaa aaagaatacg
      421 aagcaagaat tgatgctcaa aatgaaaaaa tggagacaga gaatccagaa actctagatc
      481 gagatgaaca aaatggtcaa aagaaaaaag aagatggtct ggatgttgat aaattgatca
      541 cgattgagga agcagcaaaa atcgccgtcg acaaagtagg aagcggtacg gcaacagatt
      601 gggatttgga taaagattta ggtactactt attgggaagt caaagtaaaa gacggcaatc
      661 aaagtacatc agtcaagatc aatagccaaa caggcgaagt actgtcaact gaacaagatg
      721 attaagaatt tcttcggaaa acaagagttg atttttcttt tagtcatgat acactagaca
      781 aaataacttg tttgaaggag cgatttactt gagtttagaa acgacctttg ccaatcatat
      841 ctttgccaat cctttgatga atgcatctgg tgtccactgc atgactaccc aggaattaga
      901 tgaactggct cattcagaag caggagcatt tatcactaag agctgtacga tcaatgaacg
      961 aaaaggaaat cccgaaccac gctattttga tgttccttta gggagtatca attctatggg
     1021 acttccaaac ttaggttttt cttattatct agagtatgct cttgcgtatg aaaaagtgca
     1081 ggaaaaccaa aatcaaccgt tatttttttc cattgccggg atgagtgtcc aggaaaactt
     1141 ggaaatgctt gagaaaatag aaaaaagtgg gtttaatggg atcactgaat taaatttatc
     1201 ttgtcccaat gtgcctggaa aaccacaact agcttacgat tttgaggcca cttatgaaac
     1261 actgaaagaa gtattcagca tcttttctaa gccgctagga atcaaattgc ctccttattt
     1321 tgattttgct cattttgatc agatggctga tatcttgaat cagtttccat tgacctatgt
     1381 caatgctatc aacagtgtgg gcaatggttt gtatatcgat acagaacaag aagcagtagt
     1441 catcaaacca aaagaagggt tcggcggcat tggcggagag tatatcaagc cgactgcttt
     1501 ggcgaatgtt cgcgcttttt atacacgttt gaaaccagaa atccaaatca ttggtacagg
     1561 tgggattcgg acaggacaag atgcatttga acatctgcta tgtggcgcaa gcatgttaca
     1621 gattggtacc gagcttcata aagaagggcc ggaaattttt tctagaatca taaaagaatt
     1681 aacgcagatt atgtcagaaa aaggatatac gtcaattgac gaatttaaag gcaaattacg
     1741 caccatttca tagcaaagag acgaaggtca tgattgatct tcgtcttttt ttataaaaaa
     1801 cactcgtctg ttcattcctg tgcaagcgta ctcattgtat gttagacgaa cttgtgggaa
     1861 aataacagag gcaaaaagga ggataatatg aatgatgcaa cagaattagc aaaagagctt
     1921 aaagaaaaag aatttagttt tcaagaacta gttacggata tacagaaaaa aataaagaag
     1981 caaaatcatg aattgaatgc gtttgtcgcg cttgaaccat tcgagagtaa ttcccacgag
     2041 ctattgattc cccagtcaga aaaagggcca ttggctggta tcccatttcc tttaaaaatg
     2101 ctcggtcaag aaaaaaaggg atggctagct acttcaggat ctcgtttatt tgaaacacac
     2161 cgagcttctc atacatctaa ttatgtgcgg caagcagaag cgataggtct agttcctttt
     2221 ggacaaacca atgcgccaga gtttggtttc aaaaatatta cagacccagt catctatggc
     2281 ccagcaagaa acccttggaa ccttgaatgt acaccgggcg gatcaagtgg aggagctgct
     2341 gctgcagttt catcaggaat cgttccttta gctggtgcta gtgatggcgg tggttctatt
     2401 agaatacccg cttcgttttg cggattgatt gggctgaaac cttcgcgagg gacgatgcca
     2461 gttggtccat atgcttggag aggatggcaa ggagctgcta ttgatttcgg cttgacagtt
     2521 tctatgagag acacggaggc tttattttat gggatgagaa gtatccattc cggtgcgcca
     2581 tatcaagtat cgccagttga gtggcaaaca catccaagaa aaaaacggtt gaagatagct
     2641 ttttgtgttg attcaccgat ccactcacag attagtgaag aggcagtact tgctgtaaaa
     2701 aaagctgtag atttccttgg aaaagaaggg catgatttga cagagattcc ttacccacta
     2761 gatggtcgta aactgatcac gtcttattat cagatgaatg gcgcagaaac agcagcaatg
     2821 atccgctcga ttgaaacagg catccagcga gctgtcagaa aagaagaact tgaacccatt
     2881 tcttggactt tgttccaata tggtcagaag ctctctgccg ctacttacat cgattctctg
     2941 catgtatggg atgaagcagc aattgtcatg gaaaacctac atcaagaata cgatctattc
     3001 ctttcaccaa cgactgcatt taccgcccct aaaatcagcg aagatttgca aagtgatgcg
     3061 ataagagcac agatggaaca catcagtgat tgttctgaag tagaaggttt agacgttgtt
     3121 tcttccatgt ttgaggagag cttgaagatc acaccttata cacagttggc taatctgact
     3181 ggacaaccag caatcagctt gcctactcat ttgacaaaag aaaacatgcc tctaggaatc
     3241 caatttatgg ctgccagagg aagagaagat ttgctatttc aaatcggcta tctatttgaa
     3301 gcaaatcatc agttccattt accaaatcca tggaaatagc agaaacaagg agaagaagaa
     3361 tgaagaaaaa aagattgttt gctctaataa ctagtgcctt ttttatttta agcgcttgcg
     3421 gtacagctgc cgctaatcag gaagataaca gctggagtaa tgtagaaaaa aagaaagaat
     3481 tagtagtagc gacttctggc acactgtttc ccgcatctta ttataatgat caaaatgaat
     3541 tagttggtta tgatgtagat ttggcaaaag aggtggctaa acggttaaat gtcaaaatct
     3601 cctttaaaga atacaacgtg gatggacaaa tcacttctgt ttcaaaaaga gaatcagatt
     3661 ttgctgcgaa cgattttggt atcacaaaag aaagagaaaa aaaattctcc ctttcagaga
     3721 ctttcaaata ttcatttgac agtatgatcg tacgaaaaaa agatgattca ggaatccact
     3781 cacttgaaga tcttaaaggg aaaaaagcag ctggcgaacc caatactagc tatatgcgtt
     3841 tagcggaaaa acacggagct aaattagtca catatgacaa tgccacgaat gatcagtatt
     3901 taagcgatgt agcaaatggt cgaacagatg ttattctaaa tgattattat ctgcagaaaa
     3961 tggccgtatc tgctttacca gatatccctg taaaaatact ggagaacgtt tatttcaatg
     4021 aaaacgaaag tggtttttta ttgaaagaag gaaatgatgc tctgaaaaag aaaatcgatg
     4081 gcgtattaaa agagatgaaa gaagatggga cgacaaagaa aatatctgaa aaatattttc
     4141 aaactgatgt ttcagtcaaa ccgaaagaaa cgatggctga gtcggtgagt gagtgatgta
     4201 ctttcctcta attgattttt ccctcatgtg gacttcactt cctttcgtac tgcaaggctt
     4261 ggtatacaca ttagggattg gcttcgtgtc gtttctttta ggaaatctgg tagggttgct
     4321 tttgacagtt ttaggtcttt tggattggct gcctctgaat gtatttattc gtttttattt
     4381 atcgttcttt agaggcattc ccgcactagt cctgctgttt ctgttatatt ttgggttacc
     4441 ttatcaatta agtgccttaa cggcttcagt catctgcttt acgattacca gcagtgcatt
     4501 tattggtgaa atctatcgag gatcactagc aggcgtttct tcaggtcagt gggacgctgg
     4561 tttagcttct ggtttttctt tctttggggt attgaaatat atcgttttgc cgcaagcttt
     4621 tcgaatctca gtaccggcac tcagtaacgt agctatggat ctagtgaaag gtacttcgtt
     4681 agcagcaaca atcacagtgc cagaaatatt tcaaaaagca aagatcatag gcggtcggac
     4741 attcgattat ttttctctct atattttagt tgcactgatt tattgggtca tttgtatcgg
     4801 gatcgaaaaa gtgcaacagt atttagaaaa acgttctatg catcagtcaa tctgaaaaat
     4861 agaaatggtg ctagcgtggc tggcaccatt tctatttttt gtttcttgta tattctgcct
     4921 agtattttaa aaccatttca tgtaaaatgt aaatcgaaaa tatgttcgga ggtagaaaac
     4981 atgaagaccc cgctaaccat cgaaatagaa tcctcgttat accaatattg catcgagcaa
     5041 ggcggcttgg ttgtagaaga agtcacgatg cctgatgacc aaggaattgt agatacgctt
     5101 agctgcttga caaaatttga tggaagccga gaatggcgat gctatgagct gaaagtgaca
     5161 aaagcagatt ttcgctctac agccaaactg tcatttgttg gacattataa ttattttgtt
     5221 ttaccaagta aactatatga agaagtaaaa actgaaatcc ctacaggaat aggtgtttta
     5281 gtgtatcgtc cgtacacggt tgaagaggag atgcctgcct ctggtacatt tctgattgcg
     5341 aaaaaagcag caaagaaaga gcttgctgta ccggaagaag ctttgacaaa ccgcttcatg
     5401 gcttctttat tcagggaagt aagaaaagca aagcaaatgg agcgaggcgt aaaatatttt
     5461 ccaacagatc aattgtacaa agaattgaaa agacgttcag aaaatcaaga tccattttct
     5521 aacgaacgat attatgaacg gtttttagag gaaatagcta gtcaggcaat caatgagttg
     5581 caagaagaag ttatcgcgct tcagcatgat tatgaatatt tgaggcaaca acagcagatc
     5641 cgtcggttgc ctacagagcc attggagtga aaaatatgta ctatccttat ttaagaggaa
     5701 aacaatttga cctattagca ttaaaagaag cactgtcgcg aggattgctc tccaataaaa
     5761 tccaaccagt gatcgaacca gtacgtgatt ccgctacgct taaaaatgtg atcgaattat
     5821 ttcaaaaaaa gcaccatcca atcgctgtga tccaaaatcc ccaagtagga caattcaaat
     5881 tattcgatca gcatgtgcac agttggacag tcaaagaaaa tagctctgtc gtccctgcac
     5941 agatcttaac gccagaaaat ctcagtgaag tactggattc acctccagcg tttcttgttt
     6001 tcgatggaca gcactaccca aaagatacag aggtgtggaa acagttggca ggagttgatt
     6061 cgaaattttt gataccagat actagtcgct ttcgcatctg gctgcccgaa aacaaaattg
     6121 tgatccgtga ctcttttcaa acgagaaagc atgttgaaag ttatgcagat aaaacagatg
     6181 acttttttag tgatgactat ctttttttcc acgcagacgg atatattgga ttttctgatt
     6241 tcacgatcga aggtagccgt tatttcgata aagggttccc tagccgagcg cttgctatcc
     6301 atctaacgta tattgatgct tacggcaata tacgcgtgaa acattttgtc tctgacagta
     6361 acgactctgc taaagaccaa gcattgaagt ttcttgaagc aggagataaa atgaaaaaat
     6421 ggatcatgcg tcaccatcat caactcttga ttacttcagg aatgattgaa ttattggctc
     6481 tttaccagca acaaaaattc cctggactgg gagtgttgaa aaagtggtca cttatgcatc
     6541 atttggaact tattagtcac ctgctagatc atccaaaaga ttggcttaaa tattattcaa
     6601 aagttcagga actatatgaa gtgatccaat gattagataa tgtaaatgaa aaaggaatag
     6661 aaaagaggaa actggatgta tattcggcaa acaaggaaag aagaagtaga agaagtggta
     6721 gaaatcatta atgccgcagc ggatttcatt gcttcgcaag gctctcctca atggcaaaac
     6781 gggcagaaac caacaaaaga aacgattttg aacgatattg aaagaaaaga aagctatgta
     6841 ttaatagtgg aagatcaaat tgctggaaca gctgcattag tctcaagcgt ggatcccgtt
     6901 tacacaaaaa tagatggtag ttggaaagaa tcaactggtc cttatttatc gattcatcgt
     6961 gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa aaccgttctt agaacatttg
     7021 ttgacgattg cagccagcaa aaatatccaa gatatccgta tcgatactta tccagctaat
     7081 gaaccgatgg aaaagaccat ctattctgct ggattcactt atcaagggat gattgaattt
     7141 ccttttgaac acggcgaaag aaaagcatat caaataatca tcaattgact ataaaatcat
     7201 ctgagcaaag ataaagacat taacactact gaaaaaaaca atcatatgat agccaagagg
     7261 ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat ttccgaaaat gcttctcaaa
     7321 tttttgtgga attttagcgt attttacgaa atagctgctt tctgaaactc ccttcatcga
     7381 gaggtcgtga catagctttt gtcacgacct ctcggcttat cctacgagta ttattcgata
     7441 catttatatc aataatcctt tttttatttt atctgcagct tcttggaacg ctccatcgtt
     7501 tgacacgact aaaagtgagg ggatagcttt caaacgtttc aactgtgcta aatcttctac
     7561 ataaaccgcc agtctggctg aaatctgttc agatgaatct ccacgttttt tcaggcgcga
     7621 ttgcaatgtt tctttagaga tcgtaataaa gataggacgg actaccgtcc cgaaatactc
     7681 atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa caatttccta ctgtcagttt
     7741 ctttttaatt tctgctttac tggaaccata tttatgttga gcatattggt cccactctgc
     7801 aaactcattt cccttgatca tctgattgaa tgtattgtca gaaacaaaat aataatcctc
     7861 ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa gtaatgatct tttgttccgg
     7921 ctgaaaaata gcttctgcga gtgaagtttt tccagaacca gaaggaccga taaatacata
     7981 gcaataattc attgtcattt ctcctttgat gattgaacaa gtgaaaaagc tttggaaaat
     8041 atttccaaag cttttaaaag taactttgct tttttgtata ttataccagc aagccagtca
     8101 caatagcact taaaaagctg actaaagtcg ctgttagcaa aattttcaat ccgtgttttg
     8161 cgacaagatt tcctttttct tcatttaatc ccttcatcgc accagaaatg atcccgatag
     8221 aagagaagtt ggcaaatgaa acgaggaaga cagatataat tgctgttgta cgctcagtaa
     8281 aatggaaatt accatttgct aattctgtca tagcaacaaa ttcatttgta accagtttag
     8341 tcgccatgat actaccggca tccactgctt ctttccatgg gattccgctg atgaaggcaa
     8401 gaggggcaaa gatgtaacct aggatctctt ggaaagtgat gccgaatatc ccgtggaaga
     8461 tagcattgat catggcaatc aaagcaacaa aaccgataag catagcagca accgtgatcg
     8521 ccacatggaa accatcaaga atgtattctc ccaacatttg gaaaaatgtc tgttctttgt
     8581 tttcttcaat gatcagctca tcttcttttt cttctaatac atagggattt acaattgaag
     8641 ccagaatata accgccaaat aagttcaaaa caagggcagt gatcacatat ttagaatcga
     8701 tcagtgccat ataagatccg acgatcgaca tagaaactgt agacatagcc gatacactca
     8761 tggtgaacaa acgtttctca gataggaagg gtaattcctt ttttatggaa ataaaaactt
     8821 ccgattgtcc taaaatagca gaagctactc cattataaga ttccaatttg cccaacccgt
     8881 tgattttact taatcctaga ccaacagctt tcataaataa tggaagaatc ttgatatagc
     8941 gtagaatacc gataatagca gaaataaata caattggcat caatacggaa agaaagaaag
     9001 gtgcacttcc tttatttgct acaccggcaa aaacaaagtc aacaccttgc ccagcaaaag
     9061 caagaagatg atcaaacatt tttgccaata cagaaacaac cgctgtacca aaagttgtac
     9121 gtaataatac gaaaccaaga acaagctgta aaacgaacat cacaattagt ggtttcaatt
     9181 tgattgcttt tttgttactg ctgatcaaaa aagcaagccc taatgtgaca aataaaccaa
     9241 taatacctaa taaataactc aaataatcac tcctattcca aattccgttt tattttacct
     9301 gttttgcaaa taaaatacta atctttagtg aaattttcat tactttttta gagtgaaacg
     9361 cattcatttt ccattagaaa ataaagcgaa caaaataaaa ggtttaaaaa gtgtaacgtc
     9421 ttagtaaagt ctataaaaaa acgaaactat cgattaagct aatagtttcg ttttcaacat
     9481 ttataagtaa actttttcta ttggtaatat agattttcgg aagggtagac aggtgcgcag
     9541 gtaatatcga ttcctagttt tttcaatgtt tgttcatcat ttttattgac catcacagta
     9601 gagtgggctt gtgctccctc aagttcagct agtttatcat aagcaacttg agcgattgga
     9661 ttcgttactg cactgattga taaagcgatc aagacttcat tgatgcttag tgcatccaaa
     9721 gaggaatgaa gtgtctttga tttcaatcct tgtattgttt caagaactaa tggtgacaat
     9781 aaaggaatgt catctgcgat atgggctaaa tgttttactg catttaagat aactgccgct
     9841 gacgcatcca tcaatgaact tgtacgcccg gtaatgatga cttcatctgg taattcgatt
     9901 gcaatcacag cagaaggatc ttttgtagga ctttgcgctt gaatcttatc tgcatagtca
     9961 tgggcaggga ctaccacgtt tcggtcttca ggacgtaaac cgacttcttc cataatcagt
    10021 ttgattcgat taactgcttc ttcgtcgatc aatccttttt tgaagtcgca ttctgtttgg
    10081 aaataacgac ggatgatttc ttgtttagag gcttcttgga caacggtatc atccacgatc
    10141 ccaaatccta cacgattcac acccatgtca gtaggagatt gataaacaga ttctttacca
    10201 gtaatttttt caataatacg tttgattaca ggaaatgtct caacatcccg attatagttc
    10261 acagctactt tattgtaggc atcaaagtgg aaggaatcga tcatattgac atcttttaga
    10321 tcgactgtgg ctgcttcata agcaatgttc agcggatgtt tcaacggaac attccatact
    10381 gggaaagttt cgaacttgga gtaaccagct gcatgtcctt tttggctttc atgatacagt
    10441 tggttcaagg aagtagcgag ctttccactt cctgggccag gagctgtcac tacaacgatt
    10501 ggtttagttg tttcaatata ctcatttttt ccaaaaccat cttctgagac gattttttct
    10561 acgttgactg gatagtcttc gatttctgag tgtttataga ccttgatgtt tcgtctttct
    10621 agtttattga taaagacttt cgtggcaggc tgtccactat agcgagtgat aacgacactg
    10681 ttgattaata agccataacc acgtaattca tcgattaacc gcaagatatc catatcataa
    10741 gtaatcccat aatcaccacg gatcttatta cgttcaatat ctccagcata gacgcaaatc
    10801 agtatttcag cttgatcttt caatttttgc agcaatttga ttttcgcatc ttcttcaaat
    10861 cctggaagta cacgtttcgc atgcttgtcg tcaactagct tcccaccaaa ttctaaatat
    10921 aatttgtcat agtggttcac tcgttccaaa atatacgctg actgctcttc aatatatttt
    10981 tctgaatcaa aaccaatttt tttcactagg ttccctcctt aagtactaaa gcattttttc
    11041 atttttttga ggattcgtca actggaagag cgaaattcct caaaaaaaca aagaaaatgg
    11101 tttaaaccaa cttgtctaga tagttattca ttggtatgat agtttttgat aaggaaggtg
    11161 ctacatgaaa aaaagacaga aaaagaaaaa cgcatataaa cactatatcc gttcgatttt
    11221 taccggttat gaaagaatgc ttgaagatcc cgaattggaa caattgacgt ttacttatct
    11281 aaacgaagaa acgcagctaa ctcgtgatga gcatcagcgc attcatttca caacaagaga
    11341 tctgcctagt aaataaaaaa taagataaag gtcatcggat tcgattctgt cctatcagat
    11401 ggaaagacta gttgatcgaa aaaaaggctg taccaaattt tggtgcagcc tgttttatgg
    11461 agcattgccc gctctttttt ataacaattt tttacagaaa atggtactat tgaagtatta
    11521 ataagtgaaa aacagatgag taagaggagg caagtagtat gaaagagcca gatacaaatg
    11581 caaattatga gccagacgaa ctagaggaga cgaatgcgga tcaggataca ggacaagaat
    11641 cgtcttcttt ttacgatatc aaagaaaaag caagcatgaa tctttcccaa ttattagaag
    11701 acttacaggc ttttgaaaaa gctgtccgcg aagaagatat ccctgagatc taccgtttat
    11761 acaatggccg attgaatgaa gaattaaaaa gaagctcaaa tgaaaatcac gagatcgatc
    11821 aattgctgat gcgtaagatc catgatcgtt ttttacagga atttcctttt atggaacaag
    11881 tagaaaacat ctcgccaacg atgagctatt acaaaatcgg cacttattac catgatcggc
    11941 ccacggtcgg tattgatgca agtctgccag aaatttttgt tttgcctaag atcgatgaag
    12001 agtgggagaa atattcccaa ccggagtcaa cggactatga caaaaaaatc aatgagctag
    12061 acgcaaaggt gatcactgct cagacagaaa ttgaacgttt agatgaacaa ataaaagaaa
    12121 tcaatcgaca aatgactgat ttgaacgaca ataaaggatt cttgaatcgt aaaaaaatcg
    12181 aggaagaaat tcaagaatta gagaagaaga agcaagtact atctaatgaa aaattaggct
    12241 ggcttcctta tattgagacg cctgaaacaa tccagcagca aaaagaagca ttgaagcaag
    12301 aagcaagagc agaccagctt cgtgcagcca tcgtagaaaa agaacaaagg caaatcagac
    12361 gctatttcgg tagcaaagag ggattcggtc aagcaatcca tgaattcttg atgaattatc
    12421 ttggcaatga gaatcctaat aatcaatcta atgaaggagg ttccgagtat gaatgaacac
    12481 gatgaagcag aatggacaaa aaatgaagaa caaacagaag atcatgaaga aacgaacact
    12541 tcattggaag aacaaacaag taatcctctg gatgacacag aagaaatccc tatgctagag
    12601 gaaacaaaaa tacacgaaaa agaaaaagta actccgacga atacgaatac tgcagaaaac
    12661 acccatgctt acgaaataac tgaaaatccc gggtatttag aaggaaaaga gcaagatcat
    12721 caagatatcg tcagtttgat tgaaacagcg gaagcagaat tagcggattt acgtatgaga
    12781 aaagatttga tcgaatcaga ttttccacaa ttattagaat catatgatcg ttttatattg
    12841 aatgaagaac caatctccac gatcggaaag acaagcctga tcgaatactt cacgtatgaa
    12901 ttactgaaac cgttgaatga tattggtctg gaacggtcaa attcagctta tgacacatgg
    12961 aaaacaagac atttttcgat caattatacg ttgaatggac aaaacgaatt agtcttttcc
    13021 tttgtattac cgaccatcga tcaacgatac caagtggaat cgatgagtct cctaaacgta
    13081 tcaccagaga caatggaaat caatgtccaa gatgaccggg tgctttcttt gatccgacac
    13141 tggtcagtgg atcgagtatt ctctgcaggg cagatcacga tattcaatca caaattgaat
    13201 caattattag cccatgcaag aacacttggt tttaccgtta atcagacatt actagacaat
    13261 acgaagccac ttcgtttgaa tcttcagagc gaatttgaat tgacgaatga agtattggat
    13321 gatattttta tcgtggcaat gaaaaatcca tcctacgata tggaaaagat caacgaaact
    13381 acttatcaag tacgattaga taaaggacag tctttaacga taacaacaaa cgaaaaagat
    13441 caaacagtct tgtcgatctc atctggagac tacccacgat ctgtcattga tttctttatc
    13501 aattacgaat tcttggtccc attaatggtg agaaagtcct gattggataa gttggattac
    13561 cagatgtcat tcagataatt ctgttccaaa ggacttgcgg cagttatcca gaataattgt
    13621 cacaagtctt ttctgtatgt actaaatgga cgtgaataac ttgctgatga aggagggatt
    13681 tcattaggaa tgtccacaaa taaaatagca cagcttagct tattgtctgc tgcttgtgta
    13741 gcaggtagga tcgcttttca atttattccg aatttccagc caatgactgc tattttttta
    13801 tttatagtac tttacttaag cttaaaagat gcgttaattg tcatgagtct ttctattgcg
    13861 atcagcagtt tttattttgg tgttggacca tgggtcatcg gccaatggat cagctatact
    13921 gttgttctga gtttgttttc aatcgtctgc ttgagaaaaa cagtgcaaag gaatctatgg
    13981 ttaaagggag tctgtttttt tctggcgggc atggtttacg gaataggtat gaccgttttt
    14041 gatacgctgt tatatcgttt gcctcaacct tggatctatt accttcaagg ggtgtcgttt
    14101 gatttgatgc acagtatagg caatgttgtg ttctttcttg tttttctgcc agtcgtcaaa
    14161 cgtttttaca atcattcagg aggaaaaaat gaaaaaaata atttatagtt tgcttgcaat
    14221 gagtgtcagt atactcattt tgacaagttg cagtacaacc tatacacaat cggcttccaa
    14281 ccaaaaggag gaagtaaaaa catcgcaaaa agcaacaatc acgttacaag aaaatggtca
    14341 aaacttttcg gaaaaagaag tgttcttcaa aaaaggcgat gatttactga gtattttgaa
    14401 acgaaatttt gaagtcaaag aagataacgg cttcatcact tcgcttgaag ggcactcaca
    14461 ggatgagaaa aatcaactct attggatgtt tactgtggac gggaaaagtg ctactactgg
    14521 tgccaaagag atcaaattga atgatggaca atcggtcatt ttcaatttgt ctaaactata
    14581 agtttcataa aaaactgtta aagggcgaat attgccataa ttcagtgagt tcagttgatg
    14641 aaagaaggaa aataacatgg aaaattttaa aaaaagcgga atgttttccg taattgctgc
    14701 tctaggggct aatattctag ttgctatcag caaatttgtc ggctatgcaa tcagtggcag
    14761 tgccgccatg ctaaacgaaa gcatccacag tatcgttgat tgcagcaatc agattttgct
    14821 gttattcggt gataaacaag ccaataaagg acagagcgag ttgcatcaat ttggtgaagg
    14881 acgagcaaaa tactttttca gtacaatcgt cgcaatgatg cttttcttcg gcggtggggc
    14941 acttggggtg atggaagctt ttgaaaagtt gacgcatcca tcccatgaag tgggtaatcc
    15001 gatcattgtt atagcaatac tgttatttgg tttggtaatc gaaggaagtt cgttacgcgt
    15061 ggcaatgaaa gaaatcaaag aattgaatac tgaaaaactt tccacgatgc gctttttgcg
    15121 cgaaagtcga cacagtgaaa tactgattat ttttacggaa gacttgtgtg ctgtaatcgg
    15181 tttggtcttg gctttagcag gaacaatatt gacttatgca accgggattg ctgcatttga
    15241 tgcgatcagc ggattgctga tcggtttctt gctgatggga gccgccattt tcctagcaaa
    15301 agaattttat agtctgatca tcggtgaaag cgtgacagca agcgatttat caaaaatcaa
    15361 gttagctttt gaacgtcctg agatcagtcg gctgatcgat ataaaaacag ttcatttaag
    15421 tccaacggag attttagttg cagcaaaagc agatatcgtt ggtaacaaag agaatgaagc
    15481 ttactcggtc atcaatgata ttgaaaaaaa tatgcgtcag tctcttcctg acaagaagat
    15541 gtatatatac attgaaacag ataaatatga cccaaattat tctcgtaaat aaaacgttta
    15601 ttcatgaatt gaagcaaaaa tgagaaaaat ataatcttgt ttttaaataa aacggctaca
    15661 atttagcgaa tttatgttac aatgagtccg ctgtttattt gttgtaatta aaggagatat
    15721 tttaatgaac aaaaagaaaa gtgcgccatg gcttcaagct ttgcatgcag tgatgccact
    15781 ttgtataagc tatataccag taggattagc ttgcggagta ttgttgcaaa aagttggatt
    15841 tgatcctctc ttatcaggac tgttatcgat tctggtcttt tccggtggcg cgcagttttt
    15901 agttgcatct atgcttacga cgcaagcatc atttgcaacg acattattga tggttttctt
    15961 tttagaatta cgctatacat tattaggagc aagtcttgcc ggttttatga aaaaagaaaa
    16021 gcggacgttt ttggctgtct tttctcaatc actgaatgat gaaaactatg cggtgaacta
    16081 cttaaagttt tcaactgacc cttcctggaa taaaagaaag gctttatatg tcaattggtt
    16141 ctcaatgact gcatgggccg gtagcaatat gatcggtaat tttttcggct cggtgattcg
    16201 agtagatgct gaccttgtcc attttgcttt gacggccatg ttcattttca tgtttatcat
    16261 gcagatgaaa agcgcactac tgatctttac aggtttattc tcaggcgtat taggggtcgt
    16321 attcatggta ttattccaaa atactttcgg attgattgca gcaacagtca ttgcttcatt
    16381 tgccggattt atgctggaga aggcattcaa aaaagaaaaa gctaagaaac taagagaccg
    16441 cggtaaagat gtcaatgagc cgctcttcaa cttcccggaa caggaggcca atcaccatga
    16501 ctaaactata tttgctactt ttagttgctt gtctgttcat cgtggcatat atcccgcgct
    16561 tgttcccgat gctttatttt actcatcgaa aagtgccatc ctggttcagt gactggatga
    16621 aatatgtacc ggtggcatta tttgcggcgt tagcttttaa agatgttttc attactcatg
    16681 aacatctgga tattgcttgg aatatcaaaa tagctgcaat gattctagta gcaggcgtgg
    16741 cttacaaaac acgttcgatg gcgttgtcag ttttgacagg acttgcgtct gtctttttgc
    16801 tgtctatgct ttaaactgtt ttattttatt aaaaacagag attgcgacat aagttgttca
    16861 aactgatcta tccgaactgc atttgcaaca gtcaattctg gatgcaagtt tattcgtagg
    16921 gataaaaaag attagtgttg gcttatgatg caagctcttt tttattttag gaattagaga
    16981 gggatttgtc cttctatgat tttttgctat aatagggaag atgtttattt tatgaaagaa
    17041 tagttgaggg aatttaaatg aataaaaaat tgatgcaagg aacattttgg ctaacctttg
    17101 ctaatcttct atgtaaagtt ttaggggtag tctatttgat tccctggcta agtatgatgg
    17161 ggaacaatca agatggtatg cttgctacga cattgtataa tgttggctat cttccttatg
    17221 gattgttcct gatgcttggt acagtcggat ttccaaatgc gattgcaaaa aaagtagctg
    17281 tagccacaaa agaaggagat aacgctgctt gtcggctgat ttttagaagt acgatcaata
    17341 tcatgtttgt catcggtatc gcctctgctg cactgatgta tttatttgct cctttactag
    17401 ccggaatcag tccgattgct aacgttaata atgggatact agctatcaga agtttatgcc
    17461 catctttgat tgctatccca attcttagtg caatgagagg atatttccaa ggaaaaaatg
    17521 atctgcggcc ttatggaact tctttgatta tcgaacaggc tgtgcgagtg atcgttatct
    17581 tggctggtac ttattacttg cgcgtattga ctgacggaac catattagaa gctgtattga
    17641 tcagtacagt cgcttctttc ttcggtgggt tagctgcgat tatccatatg tatatcgtcg
    17701 gacgcagaaa agacttcttc gaacttaaag attttttgat ttccagccgt tatttcgaaa
    17761 gagaaaaccg atctgcctct gtctctatca ttcgggaaac actaccgttt atttttgtcg
    17821 gctcagtcat tactatcgtt caaatgatcg accaagtaac gatgaaacca ctgcttcact
    17881 ttttccgtcc agagatcgca gatcagcaac tggaattctt attcagccgt gcttctgtca
    17941 atccaaataa attgacattg atcctgatct ccatggtagg aacagtagct ataagcagct
    18001 tgccaatatt aagcacgatg aagaaaaaag atcggataca aatcgaaaaa acagtaggag
    18061 atagtttttc gattgcgtta ttgattcttc ttccttcatt gactggaatg tctttattag
    18121 ctggaccact atacacattg ttttttggct atgatcctga aagtgtcgga tatttccaaa
    18181 tggctttgct agcttctttg tttttctctt tattcacgat cctatctacg atgcttcaat
    18241 ctttgaatca ccatcgtttt gcgatccgat taactgtaga agcaattatc ctgaaagtca
    18301 tcttccaagt aatcggatta ggattagttg gcggatacgg gatgagctta tcaaatggct
    18361 tagcattcgg tattgtcttt gttcgcggct atcactttat gtgcaaagaa taccgaatag
    18421 cacctttagc aaagatcagc aattttttct taaaaacttt ccgctccacc ctgattatgc
    18481 ttgcagtatg ttttgccgtt ttctttttgc tgaatcagcg attgtcaatg gaatcaaaaa
    18541 gctatgcaat gatttattgt gtggctgtag gaagtattgg cggcttagta tttttatttt
    18601 ctcaattcgg aaaaaatggg tttcgaatgt tgaaaaatgt taaacatcat agcagaagca
    18661 aagaataggt taactaatcg ttttttgctg tcacttgaaa aacggcaact cgtatgatta
    18721 cgcttgtcgt ttttctattt tccaatttta gtttcatatc taatcttact tatgatatag
    18781 ttataaggtc tggaaaaatt ctttgaaacg aggaaattaa acacatgaaa cttgcagtag
    18841 ttacagatag ttcggcatat ctgccggaac gtatcaagaa ccatcccgac ttattcgtta
    18901 ttccaatccc tttgattatg gatgggaaaa tttataacga aggaatcgat atcgaagcgg
    18961 atgaatatta tggactttta aaaaacagta aagattttcc tactacttca caacctgtcg
    19021 taggggaagt tttgggttta tatgaggaat tgaaagagaa agggtatgac acgattttaa
    19081 gtatccatct ttcttcaggg atttctggct ttgtcaacac attatttgcg atgaaagatg
    19141 acatcagcgg agtaacggtc attccttatg actctaagat cactagtatg ccgatgggac
    19201 acatggtaga agctgcctta gacttaaatg atgaaggtca gtcccttgat gaaatacttg
    19261 cacatatcga tcgaatccga gataatacct acgcttatct gattgtagat gatttgaaca
    19321 atttagtacg aggaggacgt ttgacaaatg gtgcagcgct gattggcagc ttattaaaaa
    19381 tcaaaccgat cctgacattt tctgaaggga aaattgtttt attcgaaaaa atccgttcaa
    19441 gtaaaaaagc atttgctcga gctgaagaaa ttattggtag aagaaataac gaaatacaac
    19501 cgccagtcaa actttacgtg atacatgcaa ataatttaga agtagcgatt gcggagaaag
    19561 agaaattgca gaaaaaatat ccaaatgcag aaatcgaaat tggtcatttc ggaccagtca
    19621 ttggtacaca tttaggtgaa aaagccatcg gcattgccat ttcagcgcaa taattccttt
    19681 tattcaaaaa acataccaaa aatattccaa catcatcttg gactttctac atgttactaa
    19741 tgagcgtgta aaagaacgag atggtgtttt tatttctatc aatgaagctc tttttctttg
    19801 caatcctcac ttcaatcctc tataatgtcg catatagttt agtaaatgag taaactacta
    19861 atttacttat ttactaaaaa taaaaggatg aggagttaaa ataatggaaa taccaacaaa
    19921 tttaaaacat aaaccagtaa tcgttgtaga agactatgat aaagttgatg gaagaaatgc
    19981 attaaatacg gatgcaaaag ggctttcact gggccttgct cagtggaatg atcgtggaaa
    20041 agtcgatatc tcaggcaagg tatggcgaca tactggagaa aaatggtcac gacagtcgga
    20101 agaattacca atcacacgta ttttagattt agcgatctta gctgcacaag gaagtttata
    20161 cttccaagat gcttatcgtt atgaaaaatt ttatgatcca gaaaatccag tcgtagacat
    20221 cattggatta caagggaacc gaatgacggt agaagtagat accaaaaatc caaagatcga
    20281 tgaagacatc attttgtatt acgatacact acaaaaagac ggagaactga ttggggaacg
    20341 attccgtatt ttaaaacgtc ttttagatga gctaggatat taatagacag agatgatcaa
    20401 aaggggaaga gaagcatgga gaatttctca ctaacagaaa accgaacctt ttcacggcta
    20461 gaaaagcaaa tgatctggaa gaaaccgaaa agccatcgga caagtgcaga agaatggcgg
    20521 atcgcctccg aaatcaaagg gaattggcaa gatcctgaaa tgaaaatcag taacgtactt
    20581 ttggaaggag atgccgggtc ggggaagaca caattagcca aagcactatc tgctgatctg
    20641 caacttccct atacaaaagt gacctgtttt gcagatatgg acaaatcaga tgttttcgga
    20701 gccttattac ctgtagtaga ttctaataag gaacaagacc aagaattact agaagctatt
    20761 taccaaacag atacacttga agctgtactg acactgatcc agaaccatta cggcattgat
    20821 cctttttctg ctaaagaaaa attagcagag ctagttcaaa ggatcgaagc tgattatcca
    20881 gatgcaatcc attacagcta ttacccttct gaaatcgttc gagcgattga aaaaggatat
    20941 ttattagaga ttcaagagcc gactgtcatc cgagatgcct ctgttcttgt ggcacttaac
    21001 tcagcgctcg aaccaaatgg gatgttgaat ttaccgacag gaatcattcg tcgccatcca
    21061 gattgcgtca ttgtgatcac tacaaatcga aattaccaag gaaatcgccc attaaatgaa
    21121 tctttaagag atcggatgca acatgcggaa aaaatggatc ttcctgcctt agaagtcatg
    21181 gcagaacgtg cgatggcgaa aactatgatc caaaaacaag aacttctctt aaagatggca
    21241 gagattatcc gtttgttgga tgaaactgca aaagctaacg ccatcaaagg tgtagcagga
    21301 atgcgttctt atttttattg ggcaaatacc tataagcaag gacaagattt actccagtcg
    21361 atttatccaa aagttttgta taaattatcc actgattcag atgaattggc aattcttgaa
    21421 caagctttgt ctgacagtgg attgctactg gaacttcagg acatattgag aaaagaaaaa
    21481 tggggtcatc ctcaatctga gcatacaaaa ggaagaacca ttagcgccga agaagcaaat
    21541 gaacgaaaca tcactgaaaa tgctgaagaa attctgcgct tagatgaaaa gaaatcgttg
    21601 tctgctgaag aaccaaatga gggagaagag aaacaagaag aaacaataga aaaaaacgag
    21661 attcctcctc aaatgcaaaa agaacgcagt agcgatatcg aaggaaaaaa tcaaaaagaa
    21721 caacaaacag gagatgattc tcaagaaatg tctgcttctg acatggaagc acatgataag
    21781 gaaatcaaga agcaactgaa taaagaagcg cgacaaatca tgaaggaaac gattcatgaa
    21841 aaagaaggtc tcatcgttca ccgaccagct ttccaaggaa aaacgccgga agcacgtgag
    21901 ctacacatgc aagtaatgcc aatagtagaa tcacttagcc ggcaaatttt agagttactg
    21961 gataatgaac aaacagagac gtaccaaaaa ggaaaatacg aaggtcagcg gttcaatgca
    22021 agcaaagtag catatggtga tttgagaaat tttgacaaaa agaacccgcc acatgagcaa
    22081 ccttctttag cggtagctgt acggatcgat gaatctgggt caatgatacg ggatgatcgg
    22141 atcgaagcgg caaaaaaagc agcgattgcc attgccgaat tttctaagaa agtcgatatc
    22201 ccattgttga tttacggaga tacagcagac cgttcgtcaa gagaaaagac ttctttgttt
    22261 tcttataaag aatttgaaga cggatttcaa tggttaaatg aaaaattagt cactatgaaa
    22321 ccaaggcaaa acaatcgtga tggtgctgca cttcgtttag cagcagaaaa attaaacaga
    22381 caatccgcaa caaccaaact gttgcttaat attagtgacg gtcagccaaa agcgttgccg
    22441 gattatacag gcaaaaaagc aaaagaagat atccagtctg ttttgaaaga atatgaacgt
    22501 caaggaatcc tatttgtctc cgcagctatt ggccaagaca aagaagaaat caagtcgatc
    22561 tacggtgaaa gtcgttttgt agatattact gacttgaatg aatttcccaa gcaaatgatt
    22621 caactgattg cccgctatct atgataaaat aaagctcaac gaacgcactt agcagattcg
    22681 ttgagctttt cgtttctagt taccggtaac aaggatactt ctaaaataga atcggttata
    22741 atcaagaggg accgttaaag gagtgaaacg atatgataca agaacaaaaa tggtggaaag
    22801 aagcagtagg ctatcaaatc tatcctgcta gtttcaaaga tagcaatggc gatggtcaag
    22861 gagacatcaa tgggatccgt gaaaaactta attatttgaa agatcttggt atcggcttta
    22921 tctggatcaa tccgatctat caatctccat ttgtcgataa tggttatgac gtaagtgact
    22981 atcaagatat agaagagaga ttcggaacga tggaagattt tgatttattg ctaaaagaag
    23041 cacatgaatt agggatcaaa gtgattatgg atcttgtcat caaccatagt tcggatcagc
    23101 atcaatggtt tgaagaatcg aaaaaatcta aagaaagccc gtatcgcgat tattacattt
    23161 gggtcgacgg ggtagatggc aaagaaccga ataattggac atcgattttc ggcggttctg
    23221 cttgggagta ttctcatgaa actggacagt attatctgca tgtttttgca aaagaacaac
    23281 cagacttaaa ttgggaaagc gagaaattga aagaagaact attcaatatg atccgatggt
    23341 ggttggataa agggatagat ggatttcgtt tagatgcgat ttctcatgta aaaaaagatg
    23401 aatattcagt aaaagccaca gagaaccctt tctcaccctt tcaaaatgtc tcaggaatcg
    23461 aagaacattt gaccgatttg aaacatgttt ttgaagagta cgatatcatg actgtcggag
    23521 aagcaagtgg tgtcactgca gaagaaggac cacaatgggt agggaaagac ggttatttcg
    23581 atatgatatt cgaatttgat catatccata tctggcagca agaaaaagaa gggcaattag
    23641 atgtccttaa attgaaactt gcattaagcg cttggcaaac ttcattggat ggtatcggat
    23701 ggaacgcttt atatatggaa aaccatgatg ttcctcgtgc cgtttcagtg tttggagata
    23761 ctcgtcccga tttttgggct atgtctgcaa aagcaatcgc tatgatgtat ttctttttgc
    23821 aaggtacgcc atttatttat caaggacagg aaatcggaat gacgaatatg ccctttgaat
    23881 cgatcgatca agttgatgca gtagattcga aaagacttta taaaaggctg cttgcggaag
    23941 gtaagacaag agaagaagca ttggatatca ttcgtgaaac aacgagagac aacagcagga
    24001 cacccatgca atggacttcg gaacaatatg ctggtttttc tacacatgaa ccttggctga
    24061 tcactaatcc aaataccaaa acgatcaatg tagaacaaca agaatatgaa ccagagtcgg
    24121 tcctgcaatt ctataagaac atgatccgca ttagacaaac caataaaggt ctgatatacg
    24181 gttcttataa agaatatctt catgaacatc cacaacttta tgtctatgaa agatatttag
    24241 aagatgaaca atatcttatt atggtaaact taacagaatc acttgctgac tatgaacttc
    24301 caaaagaagc agatcagtcc tggacattac tcttgtcaaa ttcctctagc ggagaatttg
    24361 aagctaaagg catactagca ccttatgaag ctcggttata taaaacaaac aagtaaaact
    24421 tcctcgttaa agttcattag ttggactttg cgaggaagtt tttcagattg ctctgtcaat
    24481 tactcatttc aataattcgc tctaggacca ccgatatatt taggacgaga gcgaagggct
    24541 gtcacgtgag cttgtccaag ggttttcaaa cgaggtctga caggtacttg gacatgtttg
    24601 atatgcatac cgattgatgt gtcaccgata tcgataccgg cttgtccaac tacgtgttcc
    24661 acttcgacag gatcattgaa ctggtcaaaa gcagcaacag aacaagcacc tccagcatgt
    24721 agtgcaggaa caacagagac aatttcgaaa ttttttgctt ctgccactgt tcgttctact
    24781 actaatgctc ggttcaaatg ttcacaacct tggacagcta gcgcaatttc tttaggatcc
    24841 aacaactctt ttaacgtacg gatgatccat tgaccaactt ccgcactgga attttttcca
    24901 atatgtcctc caacgacttc gcttgtgctg catcctaaaa caaaaagatc accttttttc
    24961 aagtgtgcct cagccaatat gtcattaact atttcagtca attggtcttt taattctttt
    25021 tctgacaact tcattttcta aaaccttctt tttcaattaa tattgcttcc gtgcttgaat
    25081 acgttgaaga cttgcagtta taggaaaggc aagtcccatt ccaatcagat tttggaagat
    25141 gtttcctggt atagaagcca ctccagccgg ccaatcatat aaaatccaac cggcgatacc
    25201 atagccgatg atcataacta agcttcctaa taaaagccct aatccccaca tacgagtaga
    25261 agatttgctg aaagaaccag caacatatcc ttgtaacccg tggatcacta atgaaaaaac
    25321 tgcccactgc ggatatcctg aaatcaaatc gatcaataga ccactggaag caccaacaac
    25381 taatccaccc gttggaccat aaagaaaagc agcggtatag atcccggcct cgcataatgt
    25441 cacaaatcca ttcgtagccg gtacagggat caaaattgtt aatgaaaggg caactgtcaa
    25501 cgcaatcagc atagcataga tcgttaattg cttagttttc atttctcatt cctccttttt
    25561 gaataccgcc agaccagaca cttccggtat gatcgttcat cagcacgcca ttagaaatac
    25621 aatgggcaac aaatttttta ctttctgcta ctgctttggt aaaatctgcc cctaacacaa
    25681 gattcgctgt tattgcagaa gaaaaggcac aaccggcccc gtttactgtt gtagtgtcga
    25741 tttttttatg agaaaatacc tcataatgat caattgaata gagaagatcc acagcttcat
    25801 ctccagccat ccttgtgccg cctttgatca caatgttttt tgttcctgaa gcagtgagtt
    25861 ctttagcgag atggatcatt tctgttaacg aagagggaag agcttgacca gaaagtaaag
    25921 cagcttctgc caaattaggc gtaatcatcg taacaagtgg aaaaagttct tcgatgagct
    25981 gcgaaatata caattgattg atcacagctt cagtttcttt gaatgccaaa acagggtcta
    26041 aaatgattgg gatattttgt tttgttttta tgaagtctcg aacaaccatg attgcttcac
    26101 ttgaattcaa taacccaatt ttgataccat ctaatgaagt cattttatca atcgaaacta
    26161 actgtcgttt aagcaattcc aaagatatag attccagcat aaatcgctca ttaatatcta
    26221 gtgcccctat agaagtcaag gcgcttagtc cgaatagacc aaaattctca aaagttttta
    26281 aatctgtttg gattcctcca ccagcaaatg gatcagaacc gccaattgtc aagattcttt
    26341 tcataacatt ttcctccatg aataagagta taacgaaatt ttcaaaaaag aaaaacagcc
    26401 aattggcata tttttatctc atccaattta aaaaaacaaa aagaaaagaa agaccaaaat
    26461 ggatctttct ttccttaaat agttaggact tttgtgattt taattctttc tcgatttgaa
    26521 gccgttgcac tttcattgta gctgttcgcg ggattgcatc aaaatcacgg atgataggga
    26581 cgttcaagcg aggaagttca tggacttgct cccaccatcg attccaatcc atttctttat
    26641 caggagctaa agcaataatc ggttgtggag aatgattttt atcccttaca atcactactt
    26701 ctgccaaaaa atctaacgaa tctaataaaa agtcttcaat agctaatgta ctattgatcg
    26761 tttcgatcaa atccacttgc cgatctttca aaaataggtg gccatgctta tccataaaac
    26821 cgtaatctcc gctatcccac caatcgccat aaacattttc ctggaaccgt gcatcttcct
    26881 tgtaataagt caaagcccgt cctttagaca gtaattgtat atgaccatct gtgttttcag
    26941 gtaggacatt cccaactgaa tctgtaatcc gagcgcttgt catgtcttcc agtccaacgc
    27001 ccatatctct tgcatcactt gttttcaaac tctctagtgt atgagcctta agaatcattg
    27061 gtccgcattc actttggcca tatacttgaa gaaataccgc atcctgtgcc atcgacgttc
    27121 gcaaaaatgt cttcattgtc tggttgttta tagcatcaaa agtggagtga tagtatcgga
    27181 tacccgaaaa agcttctgga tgctccttag cagtaaaacg ccactgaaca aagttattcg
    27241 gatgggtctc tagtgcaatt ggccggtgag cttccaacat atgaacaact tttgaacttt
    27301 gtgcgtttgc taaaggcatc ataggaaatc ccatggacat caaagaagag ataccaatat
    27361 tgaatctgga atgcactgga gagatgtgaa aagcgattaa tttcttttcc gaaatttttg
    27421 tgaaaactgt tttttgccat ttcgtccgcc atcccataga atgagcagaa tgacaaataa
    27481 gtttagggat accagttgta ccagaagtat gggtcatata agaaatttca tttttgtcta
    27541 aaaatgattg agaaacagac aaagcaggtt gttccattaa ataaagaata ggaa
//
LOCUS       DANKHL010000010        27592 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000010 DANKHL010000000
VERSION     DANKHL010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.012"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(20..964)
                     /locus_tag="PW101_000325"
     CDS             complement(20..964)
                     /locus_tag="PW101_000325"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL2512757.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1260..2204)
                     /locus_tag="PW101_000326"
     CDS             complement(1260..2204)
                     /locus_tag="PW101_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2512758.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2425..3639
                     /locus_tag="PW101_000327"
     CDS             2425..3639
                     /locus_tag="PW101_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2512759.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3661..5676
                     /locus_tag="PW101_000328"
     CDS             3661..5676
                     /locus_tag="PW101_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HDL2512760.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5759..7132)
                     /locus_tag="PW101_000329"
     CDS             complement(5759..7132)
                     /locus_tag="PW101_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL2512761.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7158..7442)
                     /locus_tag="PW101_000330"
     CDS             complement(7158..7442)
                     /locus_tag="PW101_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2512762.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7444..9459)
                     /locus_tag="PW101_000331"
     CDS             complement(7444..9459)
                     /locus_tag="PW101_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2512763.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9638..9943)
                     /locus_tag="PW101_000332"
     CDS             complement(9638..9943)
                     /locus_tag="PW101_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HDL2512764.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10032..11063)
                     /locus_tag="PW101_000333"
     CDS             complement(10032..11063)
                     /locus_tag="PW101_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2512765.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11270..12145
                     /locus_tag="PW101_000334"
     CDS             11270..12145
                     /locus_tag="PW101_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2512766.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12297..13139)
                     /locus_tag="PW101_000335"
     CDS             complement(12297..13139)
                     /locus_tag="PW101_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2512767.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13231..15108)
                     /locus_tag="PW101_000336"
     CDS             complement(13231..15108)
                     /locus_tag="PW101_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2512768.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15105..16526)
                     /locus_tag="PW101_000337"
     CDS             complement(15105..16526)
                     /locus_tag="PW101_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2512769.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16529..19918)
                     /locus_tag="PW101_000338"
     CDS             complement(16529..19918)
                     /locus_tag="PW101_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDL2512770.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20175..21566
                     /locus_tag="PW101_000339"
     CDS             20175..21566
                     /locus_tag="PW101_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2512771.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21639..22286)
                     /locus_tag="PW101_000340"
     CDS             complement(21639..22286)
                     /locus_tag="PW101_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL2512772.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22294..22980)
                     /locus_tag="PW101_000341"
     CDS             complement(22294..22980)
                     /locus_tag="PW101_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2512773.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(22986..24059)
                     /locus_tag="PW101_000342"
     CDS             complement(22986..24059)
                     /locus_tag="PW101_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2512774.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24281..24955
                     /locus_tag="PW101_000343"
     CDS             24281..24955
                     /locus_tag="PW101_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2512775.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            24960..26300
                     /locus_tag="PW101_000344"
     CDS             24960..26300
                     /locus_tag="PW101_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2512776.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26449..27435)
                     /locus_tag="PW101_000345"
     CDS             complement(26449..27435)
                     /locus_tag="PW101_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HDL2512777.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8042 a         5216 c         5014 g         9320 t
ORIGIN      
        1 agttcatatt cattgtttct tataatacag tgttcaatac ttctcttaaa gcacttgttg
       61 atttgctgaa cattgctttt tcttcttcgt tgattgataa ttcaacaact tcacgaacac
      121 cattttcgtc aacgattgat ggcacacctg taaagatatc tttttcgtcg tattcaccag
      181 ttaaataagc agatacaggc aatacagctt gttcgttatt caagatagct tttacgatac
      241 gtgcagtact cataccaata ccatagtaag ttgctttttt acgatcaatg atctcataag
      301 cagcattacg gactttatca gcaatcacat ctaattcatc ttgggcaata cgatggtctt
      361 tttctacaat ttcaaacact ggtttaccac cgacagttgt atgtgaccat gcagcaactt
      421 ctgaatcacc atgttcacct aagatatagc cgtggacgct gcgtgggtca acttttaatt
      481 tcaatgcgat ttctttacgg aaacgagttg tatccaatgt tgttccagta ccgataacac
      541 gtgatgttgg cagaccagat tcgttccaag cgatatatgt caaaatatct actgggtttg
      601 aagcaacaac gataataccg tcgaatcctg atcccatgat ttcttttacg atttggcgca
      661 tgattgacgc atttgtttta actaaatcta aacgagattg accaggtttt tggttgatac
      721 cagctgtgat aacaacgata tcagcatctt tacattcttc atagccacct gaccatacag
      781 ccacattttc atcgccccaa gccataccat ctaaaaggtc caatgcttcc ccttctgctt
      841 tttcttgatt cacatcgatc aatactaatt cattagagat tccttggttg atcattgcat
      901 aagcgatact tgttccaaca aaacctgtac cgacgattac tactttacga cttgtttttt
      961 tcatgaataa acagctcctt ttcataacta tttgttactt tcatgaaaag tatagtcttg
     1021 tttattcatt ttatcaacat gttaattata atagttgtta ttataatttt tgatggaaac
     1081 gaattcctga atacgcataa cagataaatg acaaatgttt acacctatat cccctgataa
     1141 tacaaccaaa agtttagaac tgaaaataaa tccaaaaaaa ttcgctgcaa cgatataata
     1201 agacagcaat cgcacctaaa acaattttcc ttcatatgct ttagtgaaag attttcttct
     1261 taatgtttat ttctccattc attgggagta catgttgaat acttcttgaa taatttgaat
     1321 aaagaagtcg tttgtttata tccgatacga ttagcgattt cttccaacga aaaactcgtt
     1381 tcttgaatca gttccaacgc aaagtcgaat tttttcttta gaaccagttc ttgaaaggaa
     1441 gcatcaaaat acattttcaa tttattacta agatagaccg gattgtagcc aaaatgagct
     1501 gctgtttctt ttaacgtaca agaagtaaaa tgcttatcga tataatctaa tactctaagc
     1561 atctcatttt ctgcgcatcc atctgctgat gaaaagcttt gttgttctaa taaatgagtc
     1621 aattcgatca gtaatagtga tgtatagaga tttaacgaac ggatcttatg actgtatgta
     1681 tcaaaataaa tcaacagcat attttcaatc aaatgcgtca aacgacttgt ttttccgccg
     1741 cgaaaatgaa tgaattgtgt atgttcccct ttttcttttg atgcatttat caaaaaagaa
     1801 gaaagtagat tatttgtgct tgccaaatga ttcaatatct ctgtagataa ggtttcttct
     1861 tttaataaga tgttgattaa aatatcctct tgccctgtgt attcgattct ttgagtagct
     1921 tctttatcaa tgatcaccac gtcactttct tccatctcta tcctttctcc attaataatt
     1981 tgtaaacatg taccgctata catataattg atttcaacaa aatcatgtgt atgctcggga
     2041 acttccgcat atcgacggtg tttactgata tgaatatttc cctgttgaaa tagatcccct
     2101 cggtggattt ttggcggtgt ctgaagatgg taagctaagt ttaaatcatt gatattcatt
     2161 ccttgttcac gctgcagagt ttcgattgat tcttcgttac gcaaaaaaaa tgtctaattc
     2221 agctagttcc aattgactcc ctcctttttg taagcggttt ctttcgctta ttagttcacg
     2281 ctgtactgct attatatgca aagctatttt tggtttcata aagactttct atggttggga
     2341 catgaacttc ctgtgattcc gctattgaga gaataaccac ttcatactat atttttgata
     2401 agcgcttaca aaaatgaaag gaacatgctt atgaataaaa agatgatgaa attttctctg
     2461 ctgtcagtct ctcttatttt gacttctgct ggtgcagtca cgtctgcaat tcccagtatg
     2521 tcagctactt atgccacgcg ttctttatct gctgttgaat tattaacgac tgctccctct
     2581 tttatggtca ctatttttgt tttgattagt agttttattg ctaggaaaat cggacaaaaa
     2641 caaactgtga tcatcggatt agtgattgca tcgatttgtg gtatcctccc cctcttctca
     2701 caaagttaca gtattgtctt agcttcaaga gctggactag gtattggctt tggattgatc
     2761 aattctttgg cagtcagcat gattgctgcc ttttatcacg gagatgaacg agcctctatg
     2821 attggttttc aaagtgcatt tcaaggattt ggtgcagctt taatgacatt tgttgcagga
     2881 cagctagtac agttcggttg gcaatatacc tttttagtct acgccatcag cttacctatt
     2941 cttgtcttgt ttgttttgtt tgttcctaca cctgaaacaa ctgaacttca atcgcatggc
     3001 acagccccaa caagacaagt aatcaaaacg aatcctaaaa tgattagtta tggtattttt
     3061 ctcttgctgg tcatcgtggt atataatgct gtatcgatta aattagccac agtcctgacg
     3121 acttattctc taggaaacga aactaatgca gcgaatatct taacaatcat gcaattggca
     3181 agtatggctg ccggagtttg ttttggctgg cttcagacaa aagcacatcg ctatatgctt
     3241 acactttctt tactactgat gggaatcggc tttgtattga ttgccactgt gataaacctg
     3301 tatgtgattg ggtttggtgc gatcctaaca ggaatttctt tttcgttatt cattccttac
     3361 ttattcaacc aagtaagtat ccaagcgccc aagtacgcgg agagtttcaa tacttcaatc
     3421 ttattggttg gcgctaattt aggtactttc attgcaccat atggtttgct ctttctctct
     3481 tttgcttctc ttgggagtaa aacacttgct gttttcgtca atggcagtat cttgctgttg
     3541 attagcgcgc ttattagcct tctagtagta cacaggacga atagaaagac agtgataaga
     3601 gtagatgaag aaataaaaga acaattgaca acaaaataaa tttgatggag gtcgtgtaaa
     3661 atgacaaatg aaaaacataa agatagatgg ctttggtatc ctggagactt tgagatccgc
     3721 catggactgc tacaaaattt ccaacgggaa gaacgaggtt ttgactggcc agcgtactgg
     3781 tacatggatg attgccatcg gaacgtcaag tttaagagat attactttct tgatcaacct
     3841 tccatgttta aagtgacgat ccaaggagtt gggtatgtag aaattaatgg acaaaaacat
     3901 ccatgtggaa aatggctgac ttgtccagct ggtaaagcaa aaattcgtat ctttgtagga
     3961 catacatcag ggctaccagc gatgtttatc gagggagacg aagtaaaatc agatattggc
     4021 tggacagcaa gtaattttat tgaagaatat ccagctggct ggagtccgct ttatgtcgat
     4081 atcgcaaaag atccaaatca aatctattat caaaaagaat gcgtccatcc agtcactgaa
     4141 acggagattg gcggaggaat cttattcgat tttgggcgag cagtgaatgg ctgtgtagaa
     4201 ctaatatctc tggatgacca tatcgaatct atcactctat gttatggaga gtccgatgta
     4261 gaagctatgg atatcgatta ttgctactat aagcaaatag gaatgacaag aaaaatgaaa
     4321 gctcgtaaac gcgcttttcg ctatttgttt ataccagagg tttctactgg aatggttcaa
     4381 cttcaagttt ttcatgaatt tttatctctt tcgaaacaag ctgctttttc aagcgattct
     4441 tctttactga atcagatctg gtcagtatca gaagaagcct tcaagttatg tagtgatttg
     4501 ttttttatcg acggtatcaa acgagatcgc tggatttggt ctggagatgc gtatcaatca
     4561 ttcatgatca atcaatatct tttttttgac gaggaaatca ataaacgaac gatattagca
     4621 ttaagaggac aaaatgaaat caagcagcat atgaatacta tcgttgacta ttcgatcttg
     4681 tggttgattg gtatcgaaaa ccactatatg atgacgaaag actatgaatt tttacgcatc
     4741 gtctacccta agatgcaatc attaatgaga tacttgatgg agcaaacgaa tgaattaggg
     4801 tttatttatg ggagagcaaa agattggata tttattgatt ggtctgaaat ggataaagaa
     4861 ggaacgcttg ctgcagaaca agtcttatta ctgaaagcct atcattccat gatcacttgc
     4921 ggagatatct tagctgaaac atatggagag aacgaaaatt atcatactca tctctatcaa
     4981 gaaaaatatc aactattgaa acaaaaccta tttgactact tttgggatga agaacttggc
     5041 gcttttatcg attgctatga atctgggaga agaaatgtga cgcgtcatgc gaatattttc
     5101 gctgttttat tcgattttgc taacaaagac caacagcaat cgatcttaaa gaatgtctta
     5161 ttgaatgaca ctatcacaca gattactact ccatatttca aattctatga acaagatgct
     5221 ttatgcaaac tcggacagac gaatatcgtc taccaaacga ttcttgatta ttggggaggg
     5281 atgttatctc atgatgcggt aactttctgg gaagaatacg accctactca aacaggaaat
     5341 gaaaagtaca gtatgtatgg agatccttat ggaaaaagct tatgccatgc atggggagca
     5401 agcccaatct atcttttagg acgctatttt atcggtttgt atccaacaaa gccaggttat
     5461 gaagaattta tgatacaacc taatctagat tcatttgaaa cactgaattg tacgctacct
     5521 atcaaagatg gaaagatttc gcttgaaaaa acaaaagata agatcgtgat tacttctgat
     5581 agaacaggcg gaacagtgaa aatgaatggt aaaagttatc ctcttctagc aaaacagcca
     5641 cttgtcttaa caagctattc aaaaataaat gcataaaaca aaaaactgct tgcgacatat
     5701 cagaagactt taaatcttct tctgatattg tccaagcaat ttttattttg aagtttgctt
     5761 aattctgttc tttagaaaca atgtttgttc tttttccggc aaaaagaaag ctagataata
     5821 cgacaagtaa aaagaaagcg atgattccaa ctgtcaaggc tatttgaccg tattctccta
     5881 gtttcccaag tacaagtcct ggtactagat aatcagtatc ggcaaatgta gaagaagcga
     5941 ttttaagatc tcctaatact ggaaggataa ataatggcag ccaactaatc aataagctat
     6001 tgaaagcaga acctaatacc gcccctcttc taccgccagt cgagttgccg aagacaccag
     6061 aagcagctcc gcagaagaaa tgtcctacta ccccaggaat gataactgta gtttgcaagc
     6121 ctatcatagc aaacatggat agtgttccta caaagaagct aacaaaaaag ccaattaata
     6181 cagcattagg tgcatatgga aaaacgatag gaacatctaa tgctggttta gaatcgggta
     6241 cgagtttttt agcaatccct tggaatgctg gaacgatttc tgccaacacc attcgaaccc
     6301 cttgaaggac gataacaaac ccagctgtga atgttccagc ttggatcagt gcaaagatta
     6361 cagcgttgtc tccattgctt agttctcttt caacaaatcc aggaccagct aacaaagcaa
     6421 gtgcgacata tacgatcgac atcaagagca tgatcgatac agtagaatct cgcaaaaagc
     6481 tgaaactttt aggaatcttt atgtcctctg tagaacggtc tttgtttcca aaaaactttc
     6541 cgatccatcc actagaagca taaccgatat ttccggtatg ccctaacgca acttgattgt
     6601 ttccagtgat gttcttcata aacggttgac aaagagcagg cgtaatagtt aatagtgttc
     6661 cttcgaagac cccgcctaat aagatcatcc acaaatttgc agtaaacccc gcgcttacta
     6721 aaatgactgc tgttagacaa gaaacataaa gcattgcttg acctgtaaga aaaatgaatt
     6781 tatatcttgt gaatctagca agtaaaatat tcacgatcat ccctactagc atgatcaaag
     6841 cagtaggtgt accgtattgt tgcaaagcaa tcgccactac tgcttcattg ctaggaacta
     6901 ctccttgagt atgcagagct gtttggaaca tcgaagcaaa aggggttagt gaattactga
     6961 ttaaaccagc accgccgatc agtactagga atccgacaaa tgttttgata gttccttgga
     7021 tcacttctga aaatttcttc ttctgaagaa cgagaccaag cagtgcaatc aaagaaacga
     7081 ggatggaggg ctgactgacg attgacacaa acaaattcaa caatttatcc attgatagtt
     7141 cctcccttga atcattttta ttcttttgac aagatcttcg ttactttttc ttttacttct
     7201 tcgctgtcaa tgatcgattg caaaggtacg agcttttcat caggtataga agttagcgta
     7261 tctttcaaag aatgctcaat gaagaaataa tcagccatat cgctagatac acttccaaca
     7321 tccgtatgac ttacttcaaa ctcgtccaca tctgctccaa gatctgcaag tgcttgtttg
     7381 atgttcatct ctaccataaa acttgttcct aatccacttt gacatactgc aaaaattttc
     7441 atatcattcg tctccttttc ttatgatcgc taatatttct tcttttgttg atgcgtgtaa
     7501 taaatcttga agattttgtt tattagaaag tagctttgtt aaatttgcca aagcccgaag
     7561 atgtgcctca ttatcgatag cagctaaaca gatgaacaaa ctaatgggat gggctggatt
     7621 atcaagcaga tccacaggtt gttgaagttt cagcaaagac atccctactt cgtttacgcc
     7681 atcttctgga cgagcatgag gtaatgcgat gtgatcgcca atatggataa acgcaccata
     7741 ctgctccacg cgctcgatca tagctttgat ataactttct tctattttat tttctgataa
     7801 tagcggctgt gcggcaaatg tgattgcttc ttgccagttt tttggttgat ccgataattg
     7861 gatcatttct tctgttaata attcagatag cataggacgt tgatcctcct tcttttctaa
     7921 aagtttgttc attttacgat ttaatacttg atagagcttt tcttcgtcta cgccttcttt
     7981 taattcgata taaggcagta gcgccgacaa caattcttgg gcagaaggca ttgaaaatcc
     8041 tggtatcagc caatctcttt gtacttgatt gatcagctga tttttttcca agtcagtcat
     8101 gattggatgc agaacataga gagacttttt cttatcagta agtgtgagag gtacagtaga
     8161 aaaaataata tcgtaagaag tttcgtcaac ctcgtgtaag cggtcagctg aattagtctc
     8221 tgtaaaaaag atcatcggga ataattgctg aagttcactt tttaagatca atgaagagct
     8281 aatgccactt ggacagacga tcacggctct tatttttcta tttctttctt ctgcgtccgc
     8341 ttttctgatc tctccgccaa atagtatagt gaaaaaacca atctcttctt cagggatgct
     8401 cttccctatt cgtctttcta acggaagtaa tgcttttctt gtcatttcgt agatggaagc
     8461 atattgatgc ttaatttgat cgatcatcac attaggtaaa taaaaagaat aggaaatacg
     8521 aaaataggca gggaccaagt gataaaagac attcatcaat aattcacgaa acgaagcaaa
     8581 ttctacggcc atcaggaatt ccatgcgatg gatgacttct gaagaacatt ccagcaaaaa
     8641 atcaagtgca ggctcctgga catctccttc aacgatcgat gctaagcaaa gagagaaaaa
     8701 tataatctcc gaattttgat gttcggaata gccaaggcca gtcaatactt gtcgactaat
     8761 gtcagtgact gcaagagaag ccaacagttc agcatctttt accagttcca tggatgtttt
     8821 tccaattcga atggttgtca aactcaaata ataagctaat tcatccattc gatttggtgc
     8881 gaatcgaaga tctgatagtg aaatcgtttt atctagcaaa tctctaactt tggcgtaata
     8941 atcaatggaa agggttgcgg ttaaatacgt gagcccaaat cttccagcaa gtgactgcat
     9001 caaacgagca gtgcgatttt ttgcttctct tcttaatatt gcttctgacc ctagcagcct
     9061 aaaaccagtt ttcctagtat acgacagctg acaatcttcc acttctaatt catttcttat
     9121 ctttttgata tctgacagga ctgtcccttt agaaacctga agaaaatctt gaaaatgaaa
     9181 aacagaaaca gacgttgcag tcgaataaac catcaaataa agaagatctc ttcgctcttg
     9241 ttcggtatac acagcttcaa atcgagtaat atccaaaaat ttttctgccc agtcgattgc
     9301 tcggaatttc tttatacgga tacgggtatc atccacatat tcggcaaaag gagttaattg
     9361 tttgatcgcc tcgcttgtat ttacttcgat gatctctgct gctttttcta gactgatttc
     9421 tcttggtgtt ttcaggagat aactaatttt gtagttcaca gcgctgttca cctcctactt
     9481 atattctagt atggtagcgc ttttaaatcg aggggtgttt ggtacaactt acagttggat
     9541 tttttttacg actgtaccga acgatttcta tcgaatggat acagaaaaaa agcaggtgac
     9601 caaatgtctc ctgcttttat gaattgattt cgtccgatca atgatgatca tgtttatttt
     9661 ttgaggtcag ttcttcaaat ttttctttga catcttcaac cgctttttcg atatcttttt
     9721 caaagtgatt gggctcatta ggctttgttt cctcttctac agaatttgaa ccagataaag
     9781 ctgtttcttt attttctgtt tcaccagagt ttgtgccagc taatgattct tctttgtctt
     9841 ctgtttcacc agaattagtt cctgctaaag ccacttcttc ttctgtttta ggggccattg
     9901 aacctcttaa tcgtttgtgt ttttcaccgt cataatttgt catgttggtt cctcctagta
     9961 gttctatact ttttcatcgt acaataagac agtttatgcg tcaaaaaaca aacccgattc
    10021 ttacaaatat gtcatatttt tccttctgat tcttgaatgc ttttgattcc ttcttccaaa
    10081 attttcattt gtaagattgt ttcttcatct ttgatgactt tttctgcgcc attaactaat
    10141 gtctcgtaaa tcccttcgta gacacggctg taatcaccaa cctcggaaat tactttttct
    10201 tcatgataac tcccttgttc atctatatag gtcaatacac cataatgttc gggcaaatcg
    10261 ataccaaagt cttcatgatc aggcatgtaa aacattttca gatgttcttc ttgtcgatct
    10321 tttgtttgtt tcacgaacat cccttttttt ccgtaaacga cgaaacttgg acgttctttg
    10381 atgcggaaat aactggattt tatcgaaatt ttcatatttc catagaaaaa atccagatcg
    10441 aagtaatcat tcattcgtcc tttccctaaa agttgacgta catcatagct gatatcatct
    10501 ggttcgccga aataagaaag aacttgatct actgtatgac aagcatgtcc atataaatag
    10561 ctgttcgcag tagaaaattc atgcgtattt tctggtactt ccggtctgaa ataatcataa
    10621 tgcatttcta cttctaaaat atcccctaat tttccgcttt cgattacttt ttggactgtc
    10681 aagaagtcag aatcgaaccg tctgttttgg taacattgga taaatagacc tttttctttt
    10741 gctaatgcaa ataactcttc tgcttccttt gacgtttctg caaaaggttt ctctactagt
    10801 acattcttac catgttcgag tgccattttt ccatattcat aatgaaattg actaggtgta
    10861 gtgatgacaa ctaattggat ctcaggatca tcccaaagat cagacagctg atcagtatat
    10921 tcgatgtctt cgaatgtttt ccacactggt tcagatgggg aaaaaatttt tttgatattg
    10981 attttgttcg taagttttct tgaaaaaggc aaatgatagc gatttgtact ctttccattg
    11041 ccaagataag cgattgtaag catgagtggt gcctcctttt attgatgttt tcagtatatc
    11101 ctagaaaagc gcagaaagta tttataatag ctattctggt aaaaaagttc agaaaaacac
    11161 ttgttatcat tcggtttggt gaaattaatg ttcaaagtaa ggataaaaat caaaaagata
    11221 gtagcggaac caagtgttta tcagtaagat agaatttgga ggtcagatca tgcttattga
    11281 agaaaaacta acaaaacaag agttgtttac cacgacagag aaacggattg tcgattacat
    11341 ccgtaggaat atcgaagctg cagtctacat gacgattgaa gaattagcaa aagctacgta
    11401 tacttcccat tcagctatca ttcgtttatg caaaaaaatg ggattcagcg gatttaaaga
    11461 attccgtttc gagctggggc gggaagttca tcaattgatt gctcagttca atcaaacgat
    11521 tgatcctaat tttccttttt caagtgaaga tagcactcag caaattgcaa agaaaatggc
    11581 ggatttatcg atccagtcaa taaaaaaagc acaattgcag attgaacatg aagatttaga
    11641 tcaaatcggt aaagtattga acaaggcaca gcgaatcttt ctttttgcaa aaggtgattc
    11701 tcaaatcacg gcaaggaaat tccagaataa aatggtcaag ttgaataagt ttttgatcat
    11761 ggctgaagaa tacagtgatt cttcttggaa tgcagccaat ttgactagcg aggattgcgc
    11821 catttttatt agctacagcg gtcgcatcca tcattatgaa cgaataatga cctatttgaa
    11881 gcatgtagag gcccctacct tgctgattac cggaaatcag cattcagaaa tggccaaaca
    11941 ggctgatatg tgtcttgtta tatcgcagga ggaatatgat tttgctaaag tagcgacttt
    12001 ctcctctcaa attgccttcg attacgtatt aaatacgtta ttctcagtga tatattctca
    12061 aaattttgaa gagaatatcc gcaacttgaa aaacaaacaa gaattgatgc aaaaaggcct
    12121 tcttaaagaa tatcggaaaa cttaagaaaa gatcctcatt tagtaataag aaagatagtg
    12181 tttgataggc tctctttgat atcatcataa aaacaggctg aagttttctt tttcaaacct
    12241 cagcctgttt gtttacggaa cgattcgaat agatatatta tagaaatgag gaatagctaa
    12301 cttctgtttc ttattttgaa cgattgacga aaaagatata taagaaggag gaaaatcagt
    12361 atacatgcag ctaacaatcc atacaggaaa aatcgtttgt tctcggatac tttctgtact
    12421 tctttttgaa cttcttttgc atgataagga acccggtgcc ctctgacaag taagcgatga
    12481 ctgttgacca tataaggtgt acaagttagg agtgtcacga aatcctgccc tttttcgatt
    12541 ctcagatctt ttgtatcagt cggttctacc gtcttgatct gatcgacttg ataagcgagt
    12601 gtccgtccat tgatttcgat atagaaagaa tcacctaaag tcagtttcgg taagtctgtg
    12661 aacaactttg cttgaggcag tcctcgatgg ctggaaataa cggcatgtgt attttctccc
    12721 cccgtaggat aagacgttcc ttccaataat gaggcacctt tttctagcag cacagtattc
    12781 gtttcatcaa aaattggcaa atggacattg atctcaggga tcgtcaaaat accgatcgta
    12841 tgtttctcaa aataggttct atcctttttc gcaggttctt ttttcttagt aaagggatct
    12901 gcacctggat ttccaccttc ttttgctaat tgctggtttt tctttgtcat gttttcttgg
    12961 attttagcca tgacttcttc attttctttg accgcttgtt gctgataatg agagatgatc
    13021 tgctgatcca gataattatt cagcgcgtcg ctaacaaatg gataagctag tatccctatt
    13081 cctgagagaa gcaagaaaag catcagcaga tctagaagtt tccttttctt tggtttcatt
    13141 tgtgttctcc ttgtgatgac tgtttgataa taaaagaaaa aggagcattt gcgtaatccg
    13201 ctgctccttt ttccaattct tgcaaaaaga ctaaatctga ctgtgcttgc gtctagcaaa
    13261 gtacagtcca gcaatcagta gaaggactac tcctgcaccg atatacacat agattccctt
    13321 accgcctgtt gaaggaagtg tacctttgtg tttatttggt attttttcag gagaaatcaa
    13381 ctgtcctgct gttacataag attgttcatc gatagtaaat gctacacggt ttgttaatgg
    13441 aacatatttt tctggcgctt tcgtttcttc cagatagtac gtgccatatt ttagacctgt
    13501 cacatcgatt aaaccgttac ttgtggttgt aaaaaccgtt gctgattctt tcgccgatac
    13561 ccagctgacg gcttttgtgg atgggtcgat cgataaatat ttcgctgtgt cactatcttg
    13621 atcacgaacg acgaattctg ctccagcaag tgtttggtct gatgtaacgt caccatctac
    13681 tttaacaaat cgtttgcccc cagtaacgac atcgactgac ggtggtgttt gatcatttgt
    13741 atgcccgtta tcgacatttg cttggttaga aaatcctttg gttggatctg ctttttcgtt
    13801 caaatgcata tagtaaacga atttcaatgt accgccagga gttaatgaag gaatataatt
    13861 cggatcaact gaaaccgtga atccgtctgt ttgctcagtg acagaataat ttactgggtc
    13921 gatttcttta tttccatcat ataaggcata agcatacgtt cctgaagaat cattatcaaa
    13981 tgttaaagca gcgtcatgag tatcgataag tttgaatgtt gtgtacttgt tttgcgttcc
    14041 ttctttatca gcgataccta atgggatatt gacagaaatc tcatattgaa ttttttcacc
    14101 gattgcgaca tcttttccat tcaattgagg tgttgtttta tcaactttag acgtatcatt
    14161 tttgatggtc ttttctactg gtgtttggct atttgccagg atctcaaaag gcgtgattgt
    14221 ttgcttttca atcatttccg cattatcagg agcttttact tcttctaaat tatattttcc
    14281 aacttctaaa tgattaacga tcaactggcc tttttcaata gatgcctcgg caaagtcatt
    14341 gttgccgatg tcataagaat gaccagtaat gaaatgtttg gctttggttt gatcagttgt
    14401 ccaagtgtac aatccatctg tgacactttg gatgtatttt ttgacgcttg gtgttccttc
    14461 ttctttagaa ataataaatt ctgctccatt tagtgcttcg ttttcggcag taccgatttt
    14521 tgtaactttc aacgttccat cattaccgac tgtattttta gggtagagat ggatagtatc
    14581 tagttcttct gtcccgtatt tataagagcc atctgcttgt ttgatcatct catatacagg
    14641 gaaagctaaa accatgtttg cggcagctga cactccgtct tttggttctt ctttgatcgt
    14701 gtagactgca tctttcccat tttgtttttt aggtaaagat aaagtgacat ttccatcagc
    14761 atctgtcgtt cctgaagcaa ctggtgtacc aggagtcaaa gactggactg cttgttttgc
    14821 tgcatccacg gacgctcctt tatctcgttg cgcataaaat tcttgagtga cgttataaac
    14881 tgaaaatgaa atatcggcta atccttggta ttgatcgaat tcgctcattt ctttcccgct
    14941 gttttggatt aaaggatcgg gtaaatcagt cattttcttt ttatgaagga tgacttgagc
    15001 tgctgtctcc tctgcaaaca ctttttttgc gccgaagctg tttgtgagta atggtaaaat
    15061 aaggaaaagg actcctaaca taacgtttat ttttttatga tttttcattt tttcactcct
    15121 gttctttttt tgataaacaa ccagattgca aagcttgcga ataccatacc aacaacgcct
    15181 aaaagtgtat ttttcgtttc gtttgttttt ggaagaaatg gacggctatt ttctttttta
    15241 ttgactactt tcaatatact gtccgcctct ttatcctcag aaagaaaagt tgaaatggta
    15301 aacggaattt ctgtttcact taaaatataa ccagaaggtg cttcgatctc ttttaatcga
    15361 taacttcctg ttttcaaccc ggaaattgaa aaagcgccat ttttatcgga aatcaggtga
    15421 agcgcatctg attcgttctt tgtccacttg tatccgttgg ctgtttgctg gaggtaattt
    15481 cctgctttgt ttttaataag gaatactgcg ccagctaatc gtttatctgg tttatttgtc
    15541 tcgattttga caaattgctt actccctgta tatacagaga ctttctttgt actgacacca
    15601 aaaccaaaat ccagtgttcc gttgttgttg atttccttgt tcgcctgtgc tgtactgctt
    15661 agctgcatct gataagaaat ggtcagtttt tttccagcat atttttccaa cgaagggatc
    15721 gaaaataaaa catcaaatcc gtgaggtttc ttgtgtatga caaagccttc tgtcagcttt
    15781 tctccattcg atgaaatcgt taaactatta ggtaaaaacg ttaatgcttc atccgcacta
    15841 tctgacaatt cgaacttttg atagtcaagg atatttacag gaattgtcgt atgcaaggaa
    15901 taagtgattt tttcaccaat cgtaaaatca tttctaggct cttctaatac cttctcaaaa
    15961 ggtggatcag ggtagtcgtt ttcttcattt ttaggataca aatggatagt tgaaagtttt
    16021 tgattgtttt gtccatgtac aggaagaaca actaccatat tctctgcctt ttctttgacg
    16081 acttctggta ctttggattc aatgaaaaga tacgctttat ctcttttttc ttgatcttta
    16141 gcggccagag aaaaagaagc gataccatct tcgttgttaa gagttgtagt tgttgcttct
    16201 gcggtaaaca taccggaaga cgcaccgttt tttgcgatct ctgcttgtgc ttcttctacc
    16261 gttttgccct tttcccgtag atggtaaaaa gaatctgtga catcataaac ttggaatgtg
    16321 acaccattta atcctcgata cgtttgtaat aaagcttttt cttgtccgtc atttggatga
    16381 tttttcggca gttgcccgtt tgggaaaagc aatttgtgta aagtaatctg aaccatttct
    16441 gtttctgttt ctgttgctac ctgagtaaaa gctggtttaa atagtaacaa gaagagacac
    16501 atactaagcc aaccaagttt tttcatattt agctgccccc tctttctttt ttcaaaaaaa
    16561 gataccaaat agaaaacgca caaccaatca tccctactag atagattcct aaacggccaa
    16621 ttcctcccgt ttcaggtaat ggtacttttg cctgattcgt gatgtctaaa gaaatctggt
    16681 tgtttttggc tcctggtgac agaacagatt catgatcttg tccatccact tgaattgacc
    16741 catcttcgtg tataactata gtaactggct cttttagacc ttggtatcct tctggtgtaa
    16801 aagtttcggt cagcgtataa gttccaggtt ttaaattttc gaatagaaag gtatctgttt
    16861 cttgtccatc ttttggcgat tctaagtcca tttctggtcc ctgcagtctg aattttgctc
    16921 cttttaatgt ctgtccgttg tcttcttttt tgtgtaccgt tagatcaaac gcttttagtc
    16981 gattttgatg agtgagcgtc catacattat ttgtcatctc ggtatttttt cccgtgtaaa
    17041 tgatttttcc atatttatcg atttggaatt cataatttcc cggcgaagta tcatatccgg
    17101 caggaccagc tgtttcaacg actcgatatt taccaggtgt gagtgaatca aaactggcaa
    17161 gacctttttc attcgttgtt tggctgtcaa ttggctgata agtaccattt gcttcttttt
    17221 tctgtaggga aaatgctgct cctttaagat ttatctcttt gtccgtccct tgcatcgcat
    17281 acttacgtac tgctacagga acttcaacaa aaggattttc aatagtcaac tggatcgtat
    17341 catcggaagt agtgactgtt tttccatcaa tggttaccgt accatcagaa gcgatcttga
    17401 tttcccaagt agtctttttg cttaatccat gcccttctgg agcttttgtt tctgtcagcg
    17461 tataggtcat ttctttttgc aatttgacat tttctggaag agaataggtt ccgtcgccat
    17521 gatctgttag taaagtatca atagaatctc ctgttaattt gaaaacagcg ccaataagat
    17581 ccttttcacc tgtagaggaa tttttcgtta ttttcaagtt taacggtgtg aattgtttag
    17641 tatttttcca tgtcattgca tctatttgtt gagaatcgta tcctggtaca ggtaattctt
    17701 tgattgtttg gtaactgaat gcttgacctt gattattgta ttgaggtagt gataatatct
    17761 cttgataact ttctccgcta tttgcagata atttgtcaat gtctgcacgt tcccacgtat
    17821 ttgttgtatc tttagctggt ttaatgattc gaatatatcc gtttttccaa gctgcagcat
    17881 ttgtcgtatg ttcccgttga atctcaaaag taacttgatc tggacgatca gctagattgt
    17941 tgtcaaattc ttcccataac tttttgatgt gaagactgac tccgggagct ttagcagatg
    18001 gtataccgaa ttcagctaat tcattggtat caatgtttgg ctggaaagtt gtcctgccgt
    18061 tcatttgata ccagaaattt ggatggaagt cctcattttc tgtttggatt ctcacttggt
    18121 aatggatttg gatttcttgg tcttttccta aatagatctg attgctcttg atggtatttc
    18181 cttctatgga aatagatgga gtgaccgttg taggacttgt ccccacactc ttgactgaaa
    18241 gtgtaccagg ctgataaatg aatggttctg cgattggatc atttatttgt ccattgctta
    18301 cagttgctga gatctgtaca gcttttttgg ctaggtattc agagatatct gttgcatgat
    18361 cagctgattc atagtaaaga tcgccttttt catctgatga aaccatttgt cgcatacgag
    18421 actctacttc tgcttttgag agaccagctg ccggatcgct ttgaagttgg atgccaagac
    18481 catgtatttc gattcctcgt tctttgagtg ccatcgcttc tccgatggtt gcgataaacg
    18541 tactgtcgat tcttctggat agattgtttt ggtcaggtgc atcatagatt cttgagatta
    18601 gagaagtatt tcccggccga tcttgcgtat tagaaaactg agttccgtaa taattgctgc
    18661 ttgattgtgc gtgtacccgc tgtactttat aggaaaatgt tggtacacca tccgtcagca
    18721 aaacgatcac ttttttatgt ccatttggaa cggatagcat gcttcctgca tctcttagtg
    18781 ctttttgtgt aaaagtacca ccatttgtcc gtgaaggtgt aatggatttt acttgatttt
    18841 tcactgaatc aaatgaaccc atctgtactg caccgttact gtagctatat ccttcgcttg
    18901 agtagccgac atatcccata ttgattttgt ctgtgatacc gctatctgct aaagtatcga
    18961 caaaacgatc gacaccaatc tttacttcac cgatccgatt attgtcgttc atacttcctg
    19021 accagtctac gaccaatacg agatcaagag gcgtgatatc tttttgtaca tttccacgag
    19081 cattcaaata aacatcaaac aatccgggtg tacttgtttc ctttgcatac tttcgtatcg
    19141 catagtctgc ttgattaccg gttcctccat attcgatata cgaatgggtc cgatcattag
    19201 gatctccatt ccaagaattt ataccatccc attgatttgt tccgttttta ttgccttgat
    19261 gatcaagaac atttgtattt cctgtaggtt gccaactggc tttaggataa gtgccctgat
    19321 catctgttgt atattctgta ggaatcgttt gattagatgg cacactcaca gctggcaagg
    19381 ataatgtagg caatgtaaat gggctgacag aattttcgtt ttcttttgat gattcttctt
    19441 ctatttcgtc tggctttggt actgacacat ctgtatcttt cttattttct gtctcatcaa
    19501 taatgtcata ttctttatta tcgacgatca gcttgtttgc ctggctgtgg taaagtgctg
    19561 gatcgattgg aaaactcaga tcgatagttt gtttagtgtt tgatgagatt tccaacgtaa
    19621 ttttcccgct atttacctga taagcatagc catctccttc ttttctttcc acatcactta
    19681 agtaattttc gactagacca atttcttttt ctatcttttc agcactggtg ttattcgcat
    19741 ttagcttgat gttcagtgct ttttttccag actgatccga aatgacttca gctgaaattg
    19801 tttctaaaac tgttggatca tctgtagtgt ctgctattgc agataatggc acaaagtttg
    19861 ataaactcaa tattatcagc atgaaaataa ctttcagttt cttccctgtg gtcgtcattt
    19921 ttcccctcct ttttcttttt ttctgttttg acgtgaatag tgaaaattat taaaataaac
    19981 aggcgttaca taattttatt tattttaata tgataattat tatacgaagg gcgctaaagg
    20041 gaatcacacc aattttcata ccaattggtt tcatttatga agttgattcc ttcgattaat
    20101 gagcgtttca ttgaaagaaa agggttgagg gagaaaaaca tatgatcagg ttgttggaat
    20161 taaactatag aaagatggaa aaaatcattc gaattctgtc catttctgac aagtggatga
    20221 ctaaaaaaga acttgccaaa cgaattggta gttcagaatc tacatttgta aggtatatcg
    20281 aagagatcaa aatccgctgg ggaacggatg taacgattca atcttcacaa aaattggggt
    20341 atcgtcttga acatttgaat gtaccagctt atttaaatgt tctgatggaa atgtcgcaaa
    20401 gttctacaaa cagtcgttta ttaaatgaga ttattgtcaa tccgggtaaa acgattgagt
    20461 attattgtga aaaaatttcc atcagccggt cttcttttac acgtaagttg aaacaatgca
    20521 atcaaatttt agaaagctat ttattaaaag tgatcgttga ccaaggtttt cagttaacta
    20581 gccttaaaag tgagctccag ttgcgtatct ttgttacctt tttcttttta agttactacg
    20641 gaagacgcac gttgccattt ccaatcgaaa aaaagaagat caaagaaatt atggtcaaga
    20701 ataattgtca gatgaatttg tttcaggaag aaagcccata tgagcaatct ttctttatta
    20761 tgtattttgt tgtgcatctc atgagagaat cccaaggaac tccatttgaa tggaaagctg
    20821 gagactctta tgtacagttg ataaatatcg ataaaaaaga cttcttaatc ttgaaaaatc
    20881 tcttaggaaa attgaagtac agcagctata taaaagtagt gaactatttt gtcaatacga
    20941 ttttttcaga attttcggag aaagaaaaaa atgaaatcag tgagcagata cgaaaaaata
    21001 tgtcctactg ttcgttttca tccgctgatt gcaaagaaaa aaacgaagaa tatgttgttc
    21061 aattattaac gcaaatttat tttttctccc gtattttccc atacgatacg actcatttga
    21121 cgatccgtgc aacttacttt gcacagcaga tgcaacaccg ccagatacgt ctatatgctg
    21181 gatttcgtgc agaacttcaa ttttattcca ctctactgaa tcagaatctg atttattatt
    21241 tatctagcta tatcttttgg atgcttatcg gtaatcctga aattcaccat tatacttctg
    21301 ataaaaaaat actgattatc agcgacttaa gtcttcgaca tagtcaatat attgaagaat
    21361 atatcagtga tattcttgct gtgcataaaa ttcactctga gacaactgct attactgaag
    21421 atcagctgtc gaaatacaat cttctcgagt atgatctgat cgttacgaat caacccattt
    21481 tgaatgccca tgttcctcat atattgattg atgatagtgt gtcattcgca aatgaagaag
    21541 aactcatccg cttattcgag ttatgaataa tcaaaaaaca gctatgtata aaccaccctg
    21601 ataaaaattt caggacaaat acacatagct gtttcttttt attgtattcc ttcagcaatc
    21661 caacgagatt tatagcgaaa gtcttttaac aactcttctt ttgtccaatt atttgcaatg
    21721 agatccgtca aagtgttcat tacgagggtc atggaaaagt ataggaaact aactgcttct
    21781 tccgacgatt gaatggagaa atgattgatt cttaataatt ccagccattt ttcgttcatt
    21841 ggagaatcag ccgatagttc tctacgtttg gttgtctgct gatcatttcg ctgggttaac
    21901 aagtataacc tttgccaata gatactttcg tgatctaagt cgcagcacca ctctaaatat
    21961 ttttcgatcc ccaaaaagaa atgttgtttg ttttcgctaa tgaattttaa gatatcttga
    22021 tggactatgt gagaactgct tttgatcgta tacaaataag catcttccaa atcttgaaaa
    22081 tatttataaa aagcaccgcg cgacatcccc atcttgtcaa cgatttctga gactttgact
    22141 tggctgatgt gctgtgagca aaatatctcc agtaataggt catcgattct ctgtttcttt
    22201 gcgttcgata aattgaaaaa agtttctttt ggcatccaca tctctccttt atctctgatt
    22261 atacgtggat agcccttcat tgtcatgaag gattcattcc ttctttttct cttcttctgt
    22321 caagtaccca tcttccattc gataaacgcg atcactccat tgaatcatac ggacatcatg
    22381 agtaaccata atagtcgctt tttgtttctc atgagcttct ttaactagta atttgacgac
    22441 ttcatatgca tgatctgtat ccaaactagc tgttggttcg tctgctaaga tcaagcttgg
    22501 ctcattatat aaagcgcgag caattgccac tctctgtctt tctccaccag aaaggtcttt
    22561 aggataacta tttttaagtg actggatatc taaagattgc agcaaggaat cgatttttgt
    22621 tgaatcttct ttttttgaag taacttggtc aactagttca aattgttcct ttaccgttaa
    22681 aaaaggaatc aaattagagc tttggagaat aaatcctatt tctttaaagc gtaaatctgc
    22741 tcgttttttt tctggaagct cagttagtga gtgaccgttg atcactattt ctccggaagt
    22801 cggtgtttgc aatcctcctg aaatggtaag gaaagtactt ttacctgaac cagaaggacc
    22861 aatgatgctg acgaattctc cttcctttac ataaaaatca attgctttca aagctgagac
    22921 ctttgtatga ccagaaccaa atgttttggt aacttttttc atcgttaata tttttttcat
    22981 ttttattaac ctcctattgc tgtaattggg tctactttgg ttatcgtacg tatcgaaaac
    23041 aatccgccta gcattgcgac aatgatcaaa atcccactat atagcaacca ttgtgaccaa
    23101 aaaacagcaa aaggcatcgc gcttggtaaa atcagactag tcaaataagc aaacaaaaga
    23161 gcaagcagaa cgcctaccac cccaacaata aaggactgtg ctactaaaga tttagcgatg
    23221 aagaaatttt tgatcccttg tgctttcatt actccaaaaa tagcagtctt ctgaagtgtc
    23281 atcacgtaca tgaaaatacc gaccacggca gcaaccacta agaacaaaaa gtatatcatc
    23341 gcatttaagg tcatattttg ggcagaatag ccaggaatac tttcaatgaa tgtcccaata
    23401 gttaataact gtagaccctt cgatgctttt tctttcgaaa ttttagctga atcctttatt
    23461 acgatcccat tgatcggctg ttcttcttca gaagcaaacg gctgttgtcc atacttcaaa
    23521 gcagtccacg tatctaggga cgcatagatg acgggagaaa ctgtgtaata agtttcagga
    23581 aagataccga caatcgtcaa aggatcgtcg taactgccaa ttttgatcgt atctccaatt
    23641 ttatagcctg aatcagccag attttgagag atgatgattt cattctttgt tttaaattca
    23701 tgccctttcg tgagttttgg taataaaaat gcttctttcg ttgtgccgaa aactgtaaca
    23761 ttttctttgt tttttccttt gattgctccg ctgtacaatc cgattggtgc tttctctcct
    23821 ccttcaatgt atttcagatc tcctcttgtt aattgtgaag ctgcaaatac ttgatttgca
    23881 tcctccgaca aaacaatctc ttgtgcgtcc caatcatcga ttgctttttt aaactcttct
    23941 gaaagtccat tcgctagtcc tgacaacatg aatacgacat acgcaatcaa gaacataata
    24001 ccgacgatca agccatatcg taatttagaa tagcgaattt ctttgatagc tagaaacatt
    24061 tgtttttcct ccctatgata gattgatagt gacaaaatgt cacttgttga atagtgtaac
    24121 tctaaagtga caatttgtca cttgtgttgt ttttttctta tcaataaatt gtctaagaca
    24181 tctttcgata aaaccttaac atgagtcata aaaaatttat ttaagcctta tttatgcttt
    24241 ccatgataaa ctaaagtgac caaatcaatg aggtgggaaa atgattaaac tattagtagt
    24301 agaagacgat aaagtattat ccaataatat aaaagaaata ctcgctgcaa tcggcgagat
    24361 aacacaagtt tatgatggta gtgaaggact gttcgagttg gaaagtggag tttatgatct
    24421 tgcaatcttg gatttgatga tgccggaaat gaatggatat gaagtgttag caaaaatacg
    24481 gaaagaaaaa atacagactc cagtattgat tttgactgca aaagatagtt tggaagataa
    24541 gattgaagga tttgaacgag gtgcagatga ttatctaaca aaacctttct atcgagaaga
    24601 actgatcatg cgcgtgaaag cattattgaa acgctcgcta ggtctctttg atgaaaatgt
    24661 tctgaagtat aaggatatca gttgtgattt aaaaagcaat gaagtcttgt ataaagaaca
    24721 agttttgccc atacaaggaa aagagtttga attgctcgtt tattttttac aaaataaggg
    24781 aatcattttg acaaaagagc agatatttga ccggatctgg ggatttgatt ctgatacgac
    24841 gattacagtt attgaagttt atatgagcaa cttacgaaaa catctgcgaa gtactggatt
    24901 ggataaagaa atcaaaacct tacgtaatgt cggttatata ctgcaaaggg tgtgatgcaa
    24961 tgaatggaga tattacacga aagcaacgta gtcgcttttt tgcgacaaat gtagcagcat
    25021 ttgcttttat cttttttctt ttgggattca ttactttaca gatattaaac caatctgcat
    25081 acagagaaac agatctgtca ttagagaaaa taaattttga ttctcgaaca gtacaattag
    25141 agatacaaag atatcagcaa ggcgatccct ttttaaaccc gactcaaacg gaacaaaaca
    25201 gaacaaacat ggaacagcca ttaatggaac catcaaatga ccgttttaac actcaagtga
    25261 tcttgtggtc taaaaatggt gagatattga ataaagcagc tattggtggg cgtttcaacc
    25321 aaatcaaaga attaaaaatg gatacgaaaa atctgaatac catccaaaat attgaattaa
    25381 atgaagacag tggctcatct gccctctctt tccgttcgat tacgaaaaaa gcggagacga
    25441 atcaagatgg cgttgcctac atccaaattt tagaaaatac aaatcaaatc aagaattcat
    25501 tagcgacttt tcgcacgatt atgattttgt gtatgattgt tttttggatt ctctctattg
    25561 gtatcagtta ttacttgtct agtctaagca tgcgccctat tattctttct tggagaagac
    25621 aaaaagaatt tgttgagaat gcttctcatg aattgcgcac accgttaacg attattcaaa
    25681 atagtttgga acatcttttt accaagccta atcatactat tattgaagaa tccgaaagca
    25741 tcgctcaagc attgtcagag acgaggcgcc tcactggatt gacttcggat ttgctgacaa
    25801 tcgctcggaa tgattctgat cagcagctgc tttcaaagca aatgatcaat acacaagaat
    25861 atatcgaaca acttgttaaa ccatttcaag agatggccat catagatgga aaagagttta
    25921 ttctccataa cgatgcaaca ttagaagtac caattgatga aaaaaaaatc catcaagttt
    25981 tggttatttt gctggataat gcattaaaat atacgaatcc agaagataag atcattttgg
    26041 attcatctgt taaccataaa gaatggctga ttcacgtaaa gaatactggc ccttctatca
    26101 gtgaagaaga tcaagcaagg atttttgatc gattttatcg ggaagatcaa tctcgggcga
    26161 aagagactgg cggatacggg cttggcttgg caattgcaaa acaggtaatc gaacagcatc
    26221 atggacagat ttctgtatcc aattatcttc cacaaggagt cgattttaaa gtccaattgc
    26281 cgttgaagcc taaaaaataa tgcttatccc taaaaaaggg ctgtgacaaa aatcttgtca
    26341 cagccctttt ttgttgcgaa taaacggtgt tccagaagta gcttcttcgg aaataagccg
    26401 aaatttcttt aagctgacca cttctgtcac aacctcttta gcttacgctt acgatttttt
    26461 attgcggtct tcaatgtgta catcaccgct tgtgtcaata tccaattctt ctcggctaac
    26521 atcttctgtc actttttcaa catcttcatg ttcttcttta tgaatcccaa cttcttcacg
    26581 aataactgta tgtttgctta catctacttg ctcttctttg attgggatag taatcgtgtc
    26641 atcttccaat ttttcgtctg ttccctgtga agattgatct gttactggtt tgcgttcgat
    26701 gacgatttct tcacgtttga ccggtacttc taccgtttca gtatcgttga ccacatgttt
    26761 gtggatgtcg acttctccag ttgtcacatc atgtgtattt acatctaatt tttcttcttt
    26821 tagttgaatt gtatcatcat ctagatttgg tttgcgatcg atgtcttcag tcttgcttgt
    26881 acccattgta tcagtctcgc caggtacgtc catcggatca gttcctggtg tcatattcaa
    26941 tggatccgtt gtattttgaa agcctgtcgt gccacctacg tccattggat ctgttccagt
    27001 gccagtttct aatccagtcc ccattgtgct tgctgggcta acagtatctt gtgtatccat
    27061 tgtatcttct gttgtttctg gctgacgata gccttttact gtgatgacaa attttccatc
    27121 agaaatatct cctctatatt gatataaagg atcattttct ggtgtagccg tctcagaatc
    27181 ataggtatca aaagaaaaag tatcttttat tttttcccat aaagaacggt cttcttcacc
    27241 atccatccga ttttcatctg tttcaacatc tataccagac aatccgtcaa atcctagtct
    27301 agatgctgct tcatcagtag tataaataac gatatcatct cgttcatacc cttcgttacg
    27361 caaccgttca attactgata atgcttcttc cctcgtacta tagcttccga tgaccgttaa
    27421 atttttctct tccatgtaaa ttcctcctag caaatagatt tatgattggt atattgttac
    27481 ttataaattt attctatagg agacgattgt tttctacaaa tatttagatt ggaatttgat
    27541 taaaacaatt taattgaaat ttgaaaacat ttatgttaat gaaagtcata tt
//
LOCUS       DANKHL010000011        27018 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000011 DANKHL010000000
VERSION     DANKHL010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.013"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(292..660)
                     /locus_tag="PW101_000346"
     CDS             complement(292..660)
                     /locus_tag="PW101_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HDL2512778.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            810..1292
                     /locus_tag="PW101_000347"
     CDS             810..1292
                     /locus_tag="PW101_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HDL2512779.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1329..2339
                     /locus_tag="PW101_000348"
     CDS             1329..2339
                     /locus_tag="PW101_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDL2512780.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2454..3167)
                     /locus_tag="PW101_000349"
     CDS             complement(2454..3167)
                     /locus_tag="PW101_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512781.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(3357..3947)
                     /locus_tag="PW101_000350"
     CDS             complement(3357..3947)
                     /locus_tag="PW101_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDL2512782.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            4431..4658
                     /locus_tag="PW101_000351"
     CDS             4431..4658
                     /locus_tag="PW101_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDL2512783.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4674..5840
                     /locus_tag="PW101_000352"
     CDS             4674..5840
                     /locus_tag="PW101_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDL2512784.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6074..7117
                     /gene="hrcA"
                     /locus_tag="PW101_000353"
     CDS             6074..7117
                     /gene="hrcA"
                     /locus_tag="PW101_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HDL2512785.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7211..7774
                     /gene="grpE"
                     /locus_tag="PW101_000354"
     CDS             7211..7774
                     /gene="grpE"
                     /locus_tag="PW101_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDL2512786.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7825..9654
                     /gene="dnaK"
                     /locus_tag="PW101_000355"
     CDS             7825..9654
                     /gene="dnaK"
                     /locus_tag="PW101_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDL2512787.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9805..10971
                     /gene="dnaJ"
                     /locus_tag="PW101_000356"
     CDS             9805..10971
                     /gene="dnaJ"
                     /locus_tag="PW101_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDL2512788.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(11053..11718)
                     /locus_tag="PW101_000357"
     CDS             complement(11053..11718)
                     /locus_tag="PW101_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2512789.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            11694..11867
                     /locus_tag="PW101_000358"
     CDS             11694..11867
                     /locus_tag="PW101_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512790.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11912..12649)
                     /locus_tag="PW101_000359"
     CDS             complement(11912..12649)
                     /locus_tag="PW101_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2512791.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12651..14153)
                     /locus_tag="PW101_000360"
     CDS             complement(12651..14153)
                     /locus_tag="PW101_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HDL2512792.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            14412..16406
                     /gene="uvrB"
                     /locus_tag="PW101_000361"
     CDS             14412..16406
                     /gene="uvrB"
                     /locus_tag="PW101_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDL2512793.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            16416..19232
                     /gene="uvrA"
                     /locus_tag="PW101_000362"
     CDS             16416..19232
                     /gene="uvrA"
                     /locus_tag="PW101_000362"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDL2512794.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            19495..20760
                     /locus_tag="PW101_000363"
     CDS             19495..20760
                     /locus_tag="PW101_000363"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HDL2512795.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            20764..21495
                     /locus_tag="PW101_000364"
     CDS             20764..21495
                     /locus_tag="PW101_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HDL2512796.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            21630..22514
                     /gene="rapZ"
                     /locus_tag="PW101_000365"
     CDS             21630..22514
                     /gene="rapZ"
                     /locus_tag="PW101_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDL2512797.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            22511..23509
                     /locus_tag="PW101_000366"
     CDS             22511..23509
                     /locus_tag="PW101_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDL2512798.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            23543..24478
                     /gene="whiA"
                     /locus_tag="PW101_000367"
     CDS             23543..24478
                     /gene="whiA"
                     /locus_tag="PW101_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDL2512799.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            25046..25702
                     /locus_tag="PW101_000368"
     CDS             25046..25702
                     /locus_tag="PW101_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512800.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            25881..26446
                     /locus_tag="PW101_000369"
                     /pseudo
     CDS             25881..26446
                     /locus_tag="PW101_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         8966 a         4539 c         5761 g         7752 t
ORIGIN      
        1 acctgaaaat aatgggcagt attatgaaag atcactgata ttttctcgta ttttttcctt
       61 attcaaaagt tttgcaggaa aattgctgaa aaaataattt tactaagtag aaaattcaaa
      121 gccattcaat cagtgatttt tttcagcaca agaaagactc cttataaaaa tagcagatac
      181 tttgtcaatt aagtgtttaa ccagggatga taaaaaggat aggattgaac aaaacctgta
      241 taaggtgatg aatagtttcc tcgccagtaa aaggtttatt cgtataaagc tttatcgcca
      301 aaatttatca tgaccttttt tatcttcaat attaaatgaa atcaattgtt caatcaattc
      361 ccagttgatt acacttttcc aaggaacttt gatgacgtta tccgtatata cgtagcctgc
      421 ttcttggata tttttttcaa gggcacgaat cgctgctttt tccggtgcga tagaaaaatg
      481 atttttagca aaactaaatg caataataaa ggtaccatgg tcagtaaaca taggttggtt
      541 ccattttaca gttgtgtcta gctggggata cgtttctgtt atcttagcaa aaaggtcttt
      601 gatcttttgt tgatgttctt tttctttgat ttgattgatg atcggctgaa atttttccat
      661 tctaaaacgc ctccattaag tggtttgatt tattttacca gaaaaacaat agttatgtat
      721 aaaaaacgaa caaaacacaa gtaatgttgt aaccgctata tttatatgat aagatgtctc
      781 aaataataaa atagaaaaga gacataaaaa tgattgactt ggcaaagaaa ttcttacacg
      841 aaaatatata ttttgagttg tatcatcatc gagccattta tacaaatgaa gatgctttgg
      901 taataaagaa agaacaagga tttacaggta cagagaccaa aagcttattc ttaaaagata
      961 aaaaagataa taactacgta tatctgactt ttactacgaa acaaacggat ttcaaagcat
     1021 tagggaagat cgtcggtaaa aggctttcta ttgtttcatc agaacgcatg gaagaacaaa
     1081 cgggacaaaa gcagggcgct gtttctccgt ttggttatga aaaacagatt ccgattatta
     1141 ttgatgaaga gctcttatca aatgaaaaac ttgtttttgc accaggtaga ccagatcaaa
     1201 cgatggtcat ccaaacggtt gacttaaaca aaatcatcca gctacttgag atgccgatat
     1261 ttttgatgcc ggcaaacgaa aaaaacaatt aaccaagtaa aatagtgcat acttggaggt
     1321 gaagcaacat gataagttat ccattattag aaagaaatgc aacgattggg gttactgcac
     1381 catcctcagg cgtatcagag gaacaccatt cgttactcga atcagcttta aagagaatgg
     1441 aagaaaaatc tttccaagta gttactggaa agacgctttg gacgcaagat aaggaaaaat
     1501 caggtccagc aagaaaacga gcagatgaat tcaatcaaat gatgcaagat gaaaatatcc
     1561 aaatgatctt tccgacttgg ggaggagaat tattgattga gattctcgat tttgtcgatt
     1621 ttgatgctct acaaccaaaa tggatagtag gttattcaga tattagtacg ttgctacttg
     1681 ctgtcacatt aaaaacaggg atagccactg cacatggaac taaccttatt gatttaagag
     1741 gagagtactt ggatgaaacg acagcaatgt gggaaagtgt actgaaaaca agagaaggag
     1801 aatcgattct acagcaatct tctgaaaagt atcaaaaagt gtggccccat gagcattcat
     1861 catcttctgt tttccatctc actgaaccaa caaattggca atcagtcagc ggagatgcag
     1921 taaggatgaa gggacgatta ttaggtggtt gtattgatac gatcaggcat ttaatcggta
     1981 ctccttatgg agatgtccaa tcttttaaaa gaaagtataa tgatggaaag ccgatcttgt
     2041 ggtttctaga aaactgtgag ctatcgtcag ttgatttaag aagatcactt gttcagatga
     2101 aactggcagg ctggtttgat ggcagctcgg ggatcatgtt cggtagaagt gcggcagagg
     2161 atttgttaga ttataaaatg aaggatgtat acgaagatct gtcaaaggaa ttaaaactac
     2221 cgatcttgta tgatattgat tgtggtcatg taccgccaca actgacattg attaatggtg
     2281 cctatgcaga agtagcggta gaagatagaa aagcgactgt tttgcaaacg tttatttaaa
     2341 aaattttttc ctgccttgga gtctatcaaa gaccaataaa tctaaataga aaagaaagtg
     2401 aaagaagctg acaaaatgct gtcagcttct ttttgtataa catattagaa agtctaatta
     2461 tctataactg tcacatcgat taactgtttc acgtcattgg gatttcctgt aggaatagct
     2521 actacagatg cattatcaat gatttcttga gttgtttctc taatgaacgc tggataagat
     2581 gtttgatctg caggcatttg ccaatgtgaa ggaactggat aactagtgat tgtcccgtca
     2641 tggttacttg aatacactaa aactccctgg taactgtatt tcccagtgag cataaccaca
     2701 tctttcggat agactgcact ccctgcatca taaggggcga taggtgttcc tgcaggataa
     2761 tgaataacat gtaattcgaa atcacccgat gtatctctaa aagtattacc tctaatagct
     2821 agccaaacac gtgcgtactc aatttgtaga tcagaatatc ctgctaacgg atctattgac
     2881 tgagaagact gtgaggaggt tgtgtctatt gttgaagaag aaatatttgt ctcaactgta
     2941 ctactagatg tagaagaagt attgggctct gagactttac tgcttgttga agatgtattc
     3001 gtttcagtag aagctgattt ctgagtactt gatttttcag ctgaactggt ttgtctgatt
     3061 gaacttttgt ctgtggtcgc agatggatca ctattttctt tactaggaga cgtacatcct
     3121 acaaggaata aagagaccaa caataaaata aaacctaact ttttcatata aaatacctca
     3181 ttattttttt aataccagta tagcataatc ctgaattttt gaatccgtaa aaccttgtct
     3241 atgaatataa atagagtgta tcttctttga taaatgtcag aaaaagtgtg acaaataatc
     3301 atcagtaaaa ttacttgtca cactattgat aaggagtatt taattgaaga gaatgattag
     3361 gagaaaggat ctccgtactt tttatagtaa tcatagaatc caagtttgta tagacgaatc
     3421 gttttttctc ttatttcttc tgtttcaaaa tggactgctt gaagaatcaa gtctgtgaat
     3481 tctataggag ctgttccatt cgcagtaatc aaattacgat ctttcacagc ttgttgttct
     3541 ataaaatctt ctttattctg ataattttcc gattctaacc aaggaaattg cgcatttccg
     3601 gtgtgtttgt aaccttgcaa taatccgctc tgagctaaat aatcaactgc cccacaaata
     3661 gcagctactg gtacgtctgt ttttaacaat ttatcgatta tttgatataa atgatgattt
     3721 tttatgtccc atgaattgcc accgatcata ataaaaaggt caactttttc aggtatttgt
     3781 tcgattaagt aatcgatttt tgtttttaat cctccaatgg aagtgacctc tgattggata
     3841 gaagcagttt tgttttgcca tttctcattt tggtttagtg tgctagtaat ataagcacct
     3901 tcccaatcgg catattcctc taatagaaac gtaatcgcag ttttcatttt caagctctcc
     3961 tttactatct ataacaagga caacagtttt aacctacaga cattcattaa aattgggtag
     4021 cgacagcgaa aacaacaatt cttaagtttt agacagagta tacagatagt caacttaatt
     4081 aaatacgaac taaataaaaa tgacaggtaa tcatttttgt ttaccagcat tataacattt
     4141 tgcatacatg gatgacaaaa gaaccaattc ccacttgctt gagtacaata ttaaaagaaa
     4201 ctggaaggca aaaagtaatt atagaaacga aaaataaatc atatcgggca ctcagacatg
     4261 aaaacaaccc tagagatata taactaggta accagtttaa tgaaagaaaa aatcatccaa
     4321 gaagtcgatt cttggatttt ttagtggatt tgttcgccaa atacgtttag tatgcgtata
     4381 tgagaggttg taagcaaagc gttgtggtct gagcacttaa ggaggaatct gtgaaaatag
     4441 cttttcatat ttttgcagat tttgacttaa tgccgaaggt cagctttgcg attaccgttt
     4501 atcgagaggc tgtaaaagca ccgtgtagct ccgatgacaa taagcaagca tttttagagc
     4561 taggtttcct tgattctaaa tatgcggctt atttcaatga gttggtgctt gaacgcgatt
     4621 ttaagaaggt cgaaaacagg cacgactttt cgacgtaagg agaaagaatt tcaatgatag
     4681 aaacaataaa tcccgcagaa aacaaggttt ctgcgtacat tcatatccca ttttgcgagc
     4741 atatctgcta ttactgtgat tttaataaag tttttttaga agggcagcca gtagatgagt
     4801 atgtggaaat gctgctgaat gaaatgaaaa taatgctaga aagtcatccg acagcgaatt
     4861 tagaaacact ttacgtaggg ggcgggacac caacttcttt atctgctaaa caacttgatc
     4921 gactgttatc aggtgctaga gagatcctgc cttttcaaaa tggtaatgag tttactgtag
     4981 aagcgaatcc aggtgatttg acccgtgaaa aacttcaagt aatgaaaaac tatggcgtca
     5041 atcgtttatc catgggagtg cagacatttg ataatcgctt attgaaaaaa attggacgta
     5101 aacacaccgc agaagatgtg tatcaaacta tgcaattttt agaagcagaa aattttacca
     5161 atgtaagcat cgatttgatt tatgcattac cgggacaaac actagaagga tatctggata
     5221 ctttggaaaa agctttggct ttggatctgc ctcactattc tttgtactca ttgatcctcg
     5281 aaaacaaaac aatgttcatg aactgggtac gacaaggaag actcgaactg cctgatcagg
     5341 aaacggaaac cagaatgttt gaagagacga ttgaagcaat ggaaaagcat ggacgtcacc
     5401 aatatgaaat aagtaacttt gggctctccg gacatgaatc tcaacataac ctcatgtact
     5461 ggaacaatgc ccactatttt gggtttggag ctggtgctag cggttatctt ggagatattc
     5521 gttaccgcaa caaaggacca atccaacact atttacgccc attacgagca aatgaacgcc
     5581 cggtattgga agaagaaaaa ttgacacgaa aaaatcaaat agaagaagaa atgttcttag
     5641 gactaagaaa aaaaacaggg attactaaaa aacatttcta tgatcgctat caaaaaacaa
     5701 ttgaatcggt gtacggtgat gtgctaaggg aattagagca agaagggtta cttgttaatg
     5761 aagaagaccg tgtttatttg acaccaagag ggaccttttt ggggaacaat gtgtttgagc
     5821 gctttttgtt tgaaaaatag tttaaagaat atttgttttt tatctgaatt taaataaaaa
     5881 aagttcaaaa atacaataaa ggactattga aacgttgata taactggatt tacatcaatt
     5941 atttagaatt agcactcttg tgtttagagt gctaaaaaat ggagtctttc tcttgacagg
     6001 agaagggctt cttgctatat ttataagtgg attagcactt agaatatatg agtgctaatg
     6061 agaggtgagg aagatgttaa caaagcgaca aaacgacatt ttacgtctga tcatccaaca
     6121 ctatacgagt agtggcgtgc ccgttggttc caaaacattg atgtctgagg gagtcaaggc
     6181 cagctcagca acgattcgca atgatatgaa agcattagaa gatgaaggat tgctgttgaa
     6241 gactcactct tcttctgggc ggattccatc tgctttaggc tatcgttatt acgtggatca
     6301 tttgttaaaa cctgctcgcg tagcagaaga tgatttgctg ctgattcgtc agtctctagg
     6361 cagagaattt catgaaatca atgagattat taagcaatct gctgagatat tatctgagct
     6421 gacaagctat acgacttttt cattgggacc tgaaataaaa gaccgccgac tgactggctt
     6481 tcgtatcgta cccttgaata atcgacagat tattgctatc gtcgtaacag atatggggaa
     6541 tgtagaaagc caagtgttca ctcttcccga atatatggga agtcaggaac tagaaaaaat
     6601 ggtacagatt gtaaacgacc gtctagtcgg agatcctctg atgacggtgt accataaact
     6661 tcgtacggag attccgatga ttcttcataa atatttccaa acgacagaag ggatgtctca
     6721 gttatttgac accgttcttg gtcatgtttt tgaagataag gtttatgtca gtggacaaat
     6781 gaatctcttg gattatgagc cccgtcaaga tcttgatcag ttcaaatcga tgttttcttt
     6841 tatgaaagac tcagacggac ttacacaaat gatcactccg atggatagca atattcatat
     6901 caagattggt gctgaattag gcaatgattt attgcagaat atgagtatga ttcaagcaag
     6961 ttacgagata gatggtcatg gaagaggtac aatagccttg ttagggccta ctagtatgcc
     7021 ttattctcgg atgttcggtc tagtcgatgt ttatcgaaga gaactagcag ctaaacttgc
     7081 tgattactac cgttcgcttg acttgtcaaa ctcgtaatgt gaataacgaa gctatttgca
     7141 gctgatcgtt ctctctttct tagtaagagc aatcaaacag ctgactgaat aatgaaagga
     7201 agactgtatc gtgaaagaaa atcaagaaga actagataaa gaaatgacag atgctcagcc
     7261 agaaccggaa atcgatgtcg aagctgcaga agattcgggt atttcagaag ctgaagcgga
     7321 agaattcgaa acagcgaaat taaaagctga gttagaagaa atggaagata aatacttgcg
     7381 ggctcgtgct gaaatcgcaa atatggcaaa tcgaggaaaa aatgagcgtg agcaattaca
     7441 aaaataccgt tcacaagatt tagccaaaaa attacttcca tcaattgaca acctggaacg
     7501 tgcattagcc acagaagtgt cagatgatca aggggcaggt ttgaaaaaag gcgtggaaat
     7561 ggtcttggaa agtttgcgta acgcactgga agaagaagga attgaaaaaa tccctgctaa
     7621 aggagaagcc tttgatccta atcttcatca agcagtacaa actgttccag cgactgaaga
     7681 tacaccagcc gatacgattg tagaagtact acaagaaggc tacaaattac atgatcgcgt
     7741 gttaagacca accatggtca ttgttgcaca ataattgtgc ggatataaaa cttacacttt
     7801 caattaaaga aggagacgtt aattatgagt aaaattatcg gtattgactt aggtacgact
     7861 aactctgcag tagcagtatt ggaaggaaac gaagcaaaaa tcatcgctaa cccagaagga
     7921 aaccgtacaa caccttctgt tgtatcattc aaaaatggag aaatccaagt aggggaagtt
     7981 gcaaaacgtc aagctgtgac aaacccaaat acgatttcat caatcaaacg ccatatcggt
     8041 gaagcaggtt acaaagtaga tgtagaaggt aaatcatata caccacaaga aatctcagca
     8101 atgatccttc aatatatcaa aggattcgca gaagactatt taggtgaaaa agtcgaaaaa
     8161 gcagttatca ctgtgccagc ttactttaat gatgcacaac gtcaagcaac aaaagatgct
     8221 ggtaagatcg ctggtctaga agtagaacgt atcgtcaatg aaccaactgc agctgctctt
     8281 gcctatggtt tagataaaac agatcgcgac gaaaaaatct tagtattcga ccttggtggc
     8341 ggtacttttg acgtatctat ccttgaacta ggagacggtg tctttgatgt attgtcaaca
     8401 gctggcgaca accatctagg tggggacgac tttgataaca aaatcatcga ctacatggta
     8461 gcagaattca aaaaagaaaa cagcattgac ttgtctcaag acaaaatggc tttacaacgc
     8521 ttgaaagacg ctgctgaaaa agcgaaaaaa gatttatctg gcgtatcaag cacacaaatc
     8581 agcttgccat ttatcacagc aggagaatct ggaccattgc atttggaaat gacattgaca
     8641 cgtgcaaaat ttgatgaatt aacagctgac ttagtagaac gtacaaaagt accagtacgt
     8701 caagcgctaa aagatgcagg tttatcacaa tcagaaatcg acgaagttat tttagtcggt
     8761 ggatctactc gtatccctgc agttgtagaa gcagtacgta aagaaacagg aaaagaacca
     8821 aacaaatcag ttaaccctga tgaagtagtt gctatgggtg ctgctatcca aggtggcgtg
     8881 atcactggtg atgtgaaaga tgttgtttta cttgacgtta caccattgtc attaggtatc
     8941 gaaacaatgg gtggagtgtt cactaaattg attgaccgca atacaacgat tccaacaagt
     9001 aaatcacaag tattctcaac tgcggcagat aatcaaccag cagtagacat ccatgtatta
     9061 caaggtgaac gcccaatggc tgctgacaac aaaactctag gaagattcca attgacagat
     9121 attccagcag ctcctcgtgg tattccacaa atcgaagtaa cattcgatat tgataaaaac
     9181 gggattgtaa atgtatctgc gaaagatcta ggtacacaaa aagaacaaaa aatcacgatc
     9241 aaatcttctt caggtctaac agacgaagaa atcgaacgta tggtaaaaga tgcagaagca
     9301 aatgcagaag cagataaagc tcgtaaagag gaagttgacc tacgtaatga tgtagatgca
     9361 ttgctattct ctgtggacaa aacattgaaa gaattagaag gcaaagtgga cgaagaagaa
     9421 gtgaaaaaag cagaaacagc tcgcgatgaa ttgaaagccg ctgttgaagc aaacaacatc
     9481 gaagaaatga aaactaaacg cgatgcattg aacgaaatcg ttcaaaactt gactgttaaa
     9541 ctatacgaac aagcggcaca acaacaagct caagaaaacc cagaagcagc tcaaggcgga
     9601 gcagatgatg tcgttgatgc tgattttgaa gaagtcgatg gcgacgataa ataatcagtt
     9661 cactgaattt cgagagaagg ccaaagttgc tactttggct ttttcttttg aaaaatagtt
     9721 tgaagtatcg gtttttgttt caaacaaaat atgatatgat aacaagcgac gtgaaaatga
     9781 tgagaaccaa taggaggaaa gcctatggca acaaaacgtg attattatga agtcttaggg
     9841 ctctcaaaag gagcatcaga agatgaaata aagaaagctt atcgtaagct ttcgaaaaaa
     9901 taccatcctg acatcaataa agaacctgat gcggaagaaa aattcaaaga agtatcagaa
     9961 gcatatgaga ttttaagtga cccgcaaaaa cgcgctgcat atgaccaata cgggcatgca
    10021 ggcacagatc caaattacgg tgctggtgga gctggtggcg gatttggtgg attcggcggc
    10081 ttctctggtg gcggcttcgg tggttttgaa gatatcttcg actctttctt tggaggaggc
    10141 ggtcggtcag tcgatccaaa tgcgcctcgt caaggtgctg atttgcaata tacggtcgac
    10201 ttgtcttttg aagaagcgat ttttggtgta gaaaaagaaa tcaagtataa tcgggaagaa
    10261 atctgtcata cttgtggtgg gaacggtgca aaaccaggca cacaaccaac tacttgtcat
    10321 aaatgtcatg gtgcaggtac gattaatgta gaacgccaaa caccacttgg acgagtgatg
    10381 agccgtcaaa cttgtgatgt ttgtcatggg acagggaaag aaatcaaaga accttgtcca
    10441 acctgtcatg gctcaggaca tgagaagaaa gcacattcag ttaaagtgaa tgtaccagct
    10501 ggtgtagaag aaggacaaca aatgcgttta gctggtcaag gagaagctgg agaaaacggc
    10561 ggaccatttg gtgatttgta tgtggtcttc cgtgtagaag aaagtgatat ctttgatcgt
    10621 gaaggttctg aaatttacta tgaattgcct ttaagttttg tacaagcagc tttgggggat
    10681 gaagtccaag taccaactgt ccatggagat gtgaaattga agatccctgc tggaacacag
    10741 acaggtacga acttccgttt acgtggaaaa ggcgctccga agttacgtgg tggtgcaaca
    10801 ggcgaccaac atgtcaaagt aaaactgatc acaccgaaaa atctaaatga acaacaaaga
    10861 gaagcattac gtgcattcgc tgaagctggc ggcatcaaag ttgaagaaca gcaagaagac
    10921 ggtttcttcg ataagtttaa agatgctttt ggcgggaaaa agcggaagta gtttttctag
    10981 taaaaacgct aagtcattaa taattaaaaa ccagacgaac aatcatatgt tcgcctggtt
    11041 tttttgtagt tgtcatgttt gtaccaacaa aaaagccagc tttagaatat gataatcgtc
    11101 atagtttcta ggggtataac cagtgagaga atggaacttg ttcaatcgat attgcaaggt
    11161 gtttttatga ataaataatt cttctgagca atttttgatg cttccgttat ttttactgaa
    11221 gcatgccaaa gtcttcttga acaaatgtac ttcttcttct gtcaggcttt tcaaaatacg
    11281 ttttacatat aatgttcgtt gatctgacgg gattgctggt aaaacgatcc cgaggtctaa
    11341 atcttcaaaa aagcggatct cgttcacaga ttgatggaaa gctatccatt cgcaaacttg
    11401 tttggcatga aggaaagatt gcggtgtctc ttgctctgta taacaaagcg acccaattcc
    11461 aaagtttagc tgtaactgat acattttatt aaactttgtc tggattttat caagcaatga
    11521 tagcaatgag gctctggaaa tccgattgac taggataatg atttgttgtc tatgtacagt
    11581 gagcaaagcc tgtccgtaat aagagaagac cctttcgagc aactccaata aaggattgat
    11641 ccgttcttga cacaattgct gattgtcaga aagttgtccc acaactacat aatatggaga
    11701 agaaaaatca agctcaatta agtgagggtg gtatcgacta tgtacttgat atcatcaatg
    11761 aaccgatggt actttcttat gcgatggaac aggtagagcg attgcttgtt tgtgcaggaa
    11821 aacaagcgat gcggctgata cgattaggtg agaaaataag aagataaaaa tacacggtcc
    11881 cttcatggcg accgtgcatt tttggttttt attaaatatt taatacttta ttcaagaaat
    11941 cttttgtccg ttcattttgc ggattttcaa agatttgttg aggcgttcca tcttctaaaa
    12001 agtttccgtc gtcaatgaac atgacgcgat tggccacttc tttagcgaac cccatttcgt
    12061 gcgtaacgat aaccatcgtc ataccttgtt ctgctaactt tttcatgacg ttcaagacat
    12121 caccgaccat ttcaggatcg agtgcagaag taggctcatc aaaaagcatg atgtcagggt
    12181 tcatcgctag agcacgagca atagccacac gctgtttttg tccaccagaa agcatttcag
    12241 gatacatatc ttttttatct tctagaccaa ctgtctgcag taatttgatt gcttttttct
    12301 ctgcttcttc tctagattct ttcttcaggt ccatcggtgc taaaatgatg ttttccaaaa
    12361 tagaaagatg agggaatagg ttaaagtgtt ggaataccat tccgatatgt tgtctcacca
    12421 agttgatatc tgtgctttta tccatcaagt gggcgccgtc aataataatt tcaccgtcag
    12481 aaggttcttc taaacgattc aagcaacgca agaatgtact ttttcctgaa cctgaagggc
    12541 caatgataca gacgacatct ccttcgttga tggatacatt gatgtcgttt aatactgtgt
    12601 tatcgccgta ttttttgact aaatgctcaa ctaagatttt ttctgccatc ttacttcacc
    12661 ttcttttcta aaacttttgc taattttgtc aatgctgtga tcaaaatcaa gtacatgatc
    12721 gcaatgatca agtaaacata cgtactttgt gttgtacgtg caacaatgat cttaccagtt
    12781 tgcagcaatt cgactacacc gattgcggaa atgatcgttg tgtcttttaa tgagataacg
    12841 aactgattga cgaatgatgg gatcatgatg cgtatcgctt gcggcaaaat gattttttgc
    12901 attgtccgat tataagaaag acctaaactt ctggaagctt ccatttgtcc gacaggaacg
    12961 gcgttgatcc cgcctcgaac aatctctgcg atataagcac ttgcgttaag agtcaaggtg
    13021 ataacaccag caaggaagtc aggaatcgta aatccaacga taccaggcaa cccaaagaaa
    13081 ataaagaacg caagaaccat cattggaatt cctcggatga tatcgacata aatactagca
    13141 attgtgcgaa gagcgcgtat tggtgctaca ctgaataatc caaaaatcac accgattact
    13201 atagccaaag caaaggagat caaagttagg agaattgttc tccaaagtcc tttcaacaat
    13261 gcttgatagt tgttttggat aagtccaaga aatgttgatt catcagcagt ggaagactca
    13321 ctggaagttt ctgaaccatc cgctacatat tctttaataa tcttatcgta ttcgcctgtt
    13381 ctttttaatt ctttcaatcc ttcattgaac atattaagaa gctcaggatt ttgtcctttt
    13441 ttaacggcaa atgcatattt tccgccagat tcttttttga tcggtgtttc caattcttgt
    13501 ccttgtttga tcgcataacc gattaccgga taatcatcca tcattgcatc gattgcgcca
    13561 acttttaacc cgtcgtacaa ttgatcagca gcatcaaaat atttgataga atagccatat
    13621 tcagattcat ggtcgcttaa aaattcagca ctttcggtac caacttttgc tccgactgtt
    13681 tttcctttca aatcttcata agacttgatc gaagtatctc cttttttgac agctaattgg
    13741 atcccgctat caaagtaaga atcagaaaaa tcatagcttt tctttcgttc atctgttaca
    13801 gtcattccgg caatcattcc gtctgcttgt cctgattcca aagcttgcaa agcagcagag
    13861 aaaccacggt agtccattcg gatgttaaat ccttgcagtt tggcgatgtt ttccatcaat
    13921 tttacatcga tgccttcgta ctcgcccttt gcgttttgaa attcaaatgg agcaaaagca
    13981 gagtcactgg caattacgta tacgtctttt ttgggctcta ttttttgcat tgtctcgctt
    14041 gtgcttgact ctgccgttgt agtgttgtca gcttgtgcaa catgaccaaa cgcaagtgtc
    14101 acaagtcctg tcattaagaa aacaaatgag tgaaataaat gctttctttt cataatcaac
    14161 ctcctaaatt tccaatattt tattattata ggcgaaaagt gctgaatcag acaagactgt
    14221 aagctcgaaa aaatgaaaaa aatgcgagtt tctaacattt tatgttataa aacataacat
    14281 gtataataag ataaaaaagt ggcgaaagta tgttcggtgt ggtataattt tttagtaagg
    14341 aatgcctttg ctttcctttt ctttttattg gtcgtaaaat gaaaaggaat gaatgaaaag
    14401 gaggacgaag aatgattgag agagatacct cccgacactt tgagcttgtc tcgaaatacc
    14461 aaccggctgg tgatcagcct gaagcgatta accaattagt ggatggcgta gtcggcggga
    14521 aaaaagcaca aatcttatta ggtgcgactg gaacagggaa aacatatacg atttccaatc
    14581 ttattgagaa agtgaataaa ccaacactga ttattgccca taacaaaaca ttggctggtc
    14641 agctgtacgg ggaattcaag gaatttttcc ctaacaatgc tgttgaatat tttgtttctt
    14701 attatgatta ttatcagcct gaagcttatg tgccttcaag cgatacgtat atcgaaaaag
    14761 attcaagtgt aaatgatgag atcgacaaat tacgacattc ggctactagt tcattattag
    14821 aaagaaatga tgtgatcgtc attgcatccg tttcatgtat ttttggatta ggctcgcctt
    14881 ttgagtacca aaaacaagta gtttcgattc gacagggagc agaactagat cgcaatcagt
    14941 tgattcgtga cttagtcagt atccaattcg aaagaaatga tatagatttt cagcgcggtc
    15001 gttttcgagt gcgcggtgat gtcgtggaga tttttccggc ttcaagagat gaacgtgcct
    15061 tacgtgtgga gttttttgga gatgaggtcg aacgtatccg agaagtcaat gcactgacag
    15121 gtgaagtttt aggagaaacg gaacacgtag caatcttccc ggcgactcac tttgtgacga
    15181 atgatgaaca tatggagcat gccgtagcga atatcaaagc ggaattagaa cagcgtttaa
    15241 ctgtcttgcg aaatgaaaac aaacttctgg aagctcaacg tttagaacag cgaaccaatt
    15301 acgatattga aatgatgtta gaaatgggct acacgtcggg aattgaaaat tattctcggc
    15361 atatggacgg acgtaaagaa ggtgaaccgc cttatacgct attggatttc tttcctgaag
    15421 attttttgat cgtggcggac gaatcgcacg taaccatgcc tcaaatcaga ggaatgtata
    15481 atggtgaccg agcgagaaaa cagatgctag tagattacgg tttccgtttg ccatctgctt
    15541 tagacaatcg gccacttcgg ttagaagagt ttgaaaaaca tgtcaatcag atcatttatg
    15601 tctctgcgac gccaggacca tatgaacatg aacaaacaga taccgttatc caacaaatca
    15661 tccgtccgac agggctgctt gacccagtga tcgaagttcg tccaatcatg ggacaaatcg
    15721 atgatctagt aggcgaaata aatgaacgag tcgaaaaaga ccagcgggtg tttgtaacga
    15781 cattgacgaa aaaaatggca gaagacctca cagattattt caaagaatta gggatcaaag
    15841 tcaaatattt gcatagtgat atcaaaacgc tggaacgaac agaaatcatt cgagatcttc
    15901 gattaggtga atttgatgtg ttagttggaa tcaatttgct tcgtgaagga ttagacgtac
    15961 ctgaagtttc ccttgttgct atcttggatg cggataaaga aggcttttta cgtagtgaac
    16021 gttccttagt ccaaacgatt ggacgagctg ctcggaatga agaaggtaaa gtaatcatgt
    16081 acgcagacaa agtaacagat tctatgcgtc tagcgatgga tgaaacgtca agacgtcgta
    16141 cgattcagca gaagtacaat gaagaacatg ggatcgttcc aaaaacgatc attaaagaaa
    16201 tccgagattt gatttctatt acgaaagaat cggaagatga tacaaaagaa gcggtacaag
    16261 tctcgtatga agaaatgacg aaagaagaaa aagatacgtt attgatgaaa ttagaaaaag
    16321 aaatgaaaaa tgcggcgaaa gcattggatt ttgaaacagc cgctaatgtg cgagatatga
    16381 ttttagaatt aaaagcttca aagtaggagg acaacatggc taacgataaa attacgattc
    16441 acggtgcacg tgcccataac ttgaaaaata tagatgtaac gattccacga gataaattcg
    16501 tggtcgttac aggtttgtcc ggttctggaa aaagttccct agcatttgat acgctgtacg
    16561 ctgaagggca gcggagatac gtagagagcc tttctgctta tgctcgtcag tttttaggac
    16621 aaatggataa gccggacgta gatagcatcg acggattgag tccggcgatt tctattgatc
    16681 agaaaacaac aagtaaaaat cctcggtcga cagtaggtac tgtgacggaa atcaatgact
    16741 atttacgctt gttgtttgca cgtgtaggac atccgatttg tcccaacgat catatcgaga
    16801 ttaccagtca atcagtagat caaatggcag atcaagtaat ggctttgcct gagcgcacaa
    16861 agatccaagt gttggcaccg atcgtggtaa agaaaaaagg acaacataaa aaaatattcg
    16921 aacgaatcca aaaagaggga tacgtccgcg ttcgagtcga tggtgagatc tatgatttaa
    16981 gtgaagtgcc tgagctggag aaaaacaaaa aacatgatat cgcgatcgtc atcgaccgga
    17041 tcattgtgaa agaagggatt cgttctcgtc tatttgattc tttagaggcg gctctgcgat
    17101 tggcagacgg ctatgctctg gttgacgtga tcggtgaaga agaaatgttg tttagcgaac
    17161 actatgcctg tccttattgc ggatttactg ttggcgaact agaaccaaga ctgttctcat
    17221 tcaatgcgcc ttttggagct tgtccagact gtgatggttt aggtgtcaaa ttagaagtcg
    17281 atcaagattt agtgattcca gatagaacga agacacttag tcaaggtgct atcgttccat
    17341 ggaatccaat cagctctcag tattatcctc aaatgttagc gcaggcttgt gaaagtttcg
    17401 gtatcgattt agatacacca ttcgaagaat taccggaaga gcatcaggat atcatcttga
    17461 atgggacaga tgatcttttc catttccatt atgaaaatga ttttggtggc gttcgcgatg
    17521 tagaaacgac tttcgaaggg gtattgaaga atatcgagcg tcgttatcat gatacgaaca
    17581 gtgatttcac gcgtgagcaa atgcgtcttt atatgacaga attaacttgt cagacatgta
    17641 aaggttatcg attgaatccc caagctctat ctgttcaaat caatggaacg aatatcgggc
    17701 aaacaaatga gttgtctatt caaaaagcag tagatttctt ctcaaactta acgttatctg
    17761 aacaagagaa agtaattgcc aaaccgattt taaaagaagt aaatgacaga ctttcattct
    17821 tggaaaatgt gggactagac tatctaactt tgagtcgcgc ttccggaaca ttatctgggg
    17881 gagaagcgca gcgaatcaga ttggctacac aaattggatc gaatctttct ggagttttgt
    17941 atattttaga tgaaccatcg atcggattgc atcaacgcga caacgatcga ctgattggct
    18001 cattaaagaa aatgcgtgat cttggcaaca ccctcgttgt agtcgaacat gatgaagata
    18061 cgatgtgggc agcagattat ctgattgacg tcggacctgg ggccggagaa caaggcggac
    18121 aaatcgtggc tgcaggcact cctgaagaag tagctaatga tgaaaattct ttgacaggga
    18181 aatatctttc agggaaaaaa tcaatccctg taccaaaaga acgccgtaaa ggtaatggga
    18241 aagcaatcaa gatcactggt gcttcagaaa acaatctgaa aaatattagt gttgaatttc
    18301 cattaggaga atttgtagca gtcacaggtg tgtctggttc tggaaagagt acattggtca
    18361 atagtatctt aaagaaatcg ttagcgcaaa aattaaataa gaattctgct aagccaggta
    18421 aattcaagac aatttccggc tacgaaagta tcgaaaagat catcgatatc gatcaaagcc
    18481 caatcggccg gacgccgaga agtaatccag cgacttatac aagtgtattt gatgatatcc
    18541 gtgggttatt tgctcaaacg aacgaggcaa aaatgcgggg ttataagaaa gggcgtttta
    18601 gtttcaacgt aaaaggcggt cgttgtgaag cttgtcgcgg ggatggaatt attaagatcg
    18661 aaatgcactt tttgcctgat gtctatgttc cttgtgaagt atgtcatggc aaacgatata
    18721 actctgaaac attagaagtg cattacaaag gaaaaagcat tgctgatatt ttggaaatga
    18781 cagtagaaga tgctgtagaa ttcttcaagc acattccaaa gattcatcgc aaactgcaaa
    18841 cgattgttga tgttggctta ggttatgtga cgatggggca accagcaacg acattgtccg
    18901 gtggtgaggc acaacggatg aaacttgcca gtgaattgca caaaatctct aatggaaaga
    18961 atttctatat actagatgaa ccaacgacag gacttcatag cgatgacatc gcccgcttgt
    19021 tgcatgtatt acaaagatta gtagatgctg gtaacacagt tttagtgatt gaacacaatc
    19081 tagatgtaat caaaacagca gattatatca ttgatttagg accagaaggt ggagaaggtg
    19141 gaggaacgat ccttgcgact ggaacaccag aagaaatcat taacgtaaaa gaaagttata
    19201 caggtcacta tttgaaaaaa ataatggtat aaagaggata gatagctgag atagttaaaa
    19261 aaacataaaa aaatgaattt ttgcttgttt tattaatatc agtgttctag aatcattaca
    19321 tggtagttct ttctacactg attggtatag acaaaaactt cctataagtt caatccgttc
    19381 aaaaaagaat tgagcaatcg gaggagtttc ctttgattgc ttttttcttt atggtatgat
    19441 attccttgtg tagttggaaa agaaaaacaa gagattatga gtaagtgaga gataatgatg
    19501 aacagtattg aaaatgaaga atttatccct atattattag gaagcgacat gaacgtttat
    19561 ggtatggcac gttcttttaa tgaagcatac ggcaaaatct gccaagcata tgcttcggat
    19621 cagttagccc ctacccgcta cagtaaaatt gtcaatgtag aagttattcc aggatttgat
    19681 aaagaccctg tgtttatcga gacgatgctt cgtttggcaa aagaaagata ttctgataag
    19741 agtaaaaaat atcttttgat tgcttgtggt gatggatatg cagaattgat ctcccaacat
    19801 aaacaagagc tgtcagaata ttttatctgt ccatatattg attattcttt atttgaaaga
    19861 ctgatcaata aagtaagctt ttatgaagtt tgtgaagaat atgatcttcc gtatcctaaa
    19921 acattgatcg tcagagaaga aatgttagta aatggacatt tggaacagga attgcctttt
    19981 gaattcccag ttgctttgaa acctgcaaac agtgtggagt atctttctgt ccaatttgaa
    20041 ggaagaaaaa aagcttttat tttagagact agagaagagt tcgatttaat tttaggccgg
    20101 atatacgaag ccggttataa aagtgaaatg attgtacaag attttatccc gggtgatgat
    20161 agtaatatgc gtgtgctaaa tgcgtatgta gatgaagatc atcaagttcg gatgatgtgt
    20221 cttggtcatc cactgttaga agatcctaca ccagcatcga ttggtaatta cgttgtgatc
    20281 atgccagatt ataacgaaaa aatctatcaa accattaagg catttttgga aaaaatcgaa
    20341 tatacaggct ttgccaattt cgatatgaag tatgatccta gagatggaga atataaatta
    20401 tttgaaatca atttgagaca aggtcgtagc agtttctttg tgacattgaa cggcttgaat
    20461 ctggctcgtt tcgtcacaga agatcgagtg tttaacaaac cctttgttga aacgacctat
    20521 ggaacgaatc aatcagataa agcaagatta tggatgggtg tgcccaaaaa aatcttttta
    20581 gaatatgctc gggaaaatga agacaaaaaa ctagctgagc aaatgatcaa agaaaatcgt
    20641 tacggtacta ctgtgtttta tgaaaaagac cggtcaatca agcgctggtt gttgatgaaa
    20701 tatatgttcc ataactatat tccaagattc aaaaagtatt ttcatgtgaa agaaggctag
    20761 ttcatggaga attttttcag tattttaggc ggaatgggca cgatggcgac agaaagtttt
    20821 gttcgcttga tcaaccaccg aacgaaagct acaaaagatc aagaatattt gaattatgtc
    20881 ttattcaatc atgcaacagt tcctgatcgt acagcttata ttttagatcg atcagaagaa
    20941 aatccaatgc cgtttctgct ggatgatatt gagaaacaaa atttattgca gccgaacttc
    21001 attgtgttga cttgtaacac cgcacactac ttttttgaag aattacaagc ggcaacggat
    21061 attcctatcc ttcatatgcc aagagaagca gcaaatgaac ttgtccgtca gcatacgaca
    21121 gggagagtag cgattctagg aacagaagga agtatgaaag ctggaatcta tgaacgcgaa
    21181 gtcaaaaatc ttggattcga aacagtgatt cctgatactg ttcttcaaga aaaaatcaat
    21241 tatctgatct atcatgagat caaggaatcg gattatttga atcaagagct atattacgaa
    21301 atcttagaag aagcggtaga gcgcctaaac tgtgaaaaag ttatcttagg ttgcacagaa
    21361 ttgtcattga tgcatgaatt tgctgaagat aaccattatc cagtgatcga tgcccaatct
    21421 attttagcag atcggacgat tgaacgagcg ctagctgaaa gaagcaaggc attggataca
    21481 gcatcggaaa agtaaaaaaa ttggatttgg ggcaaatatc ctaaaagata tttggtccaa
    21541 atctttttgt aaaagaaaaa gaaaactggt aatgatacgc ccggaatgat ataattttac
    21601 tatgcacaat gtgagaggag ttcgtaatta tgacagataa tttgcaatta gtcatcataa
    21661 caggtatgag cggtgcaggg aaaacagtag ctatccaaag ttttgaagat atgggctatt
    21721 tttgtatcga taatatgccg cctcgattga ttccaaaatt ctgggaatta atcaaagaat
    21781 caggcaaagt cacaaaaatg gctttaggag tggatttacg ttctcgttcc ttctttgaag
    21841 aaatccaaaa catgctgatc gaaatcgaaa atacgaactt catcgacact tctatcttat
    21901 ttttagatgc ttcagacgaa gaactagtgg ctcgctacaa agaaacaaga cgagcacatc
    21961 caatggcgat ggacggtttg atcactgaag ggatacgtaa agaacgggcg atactagacg
    22021 acttgaaagc ccaagcttcc gtgatcattg atacgacgac attgacacca cgacaattac
    22081 gtgaaaaaat caatgagtca ttcaaatcaa agggagacca aggatttcgg atcgaatttg
    22141 tatcatttgg ttttaaatac gggttgccga tcgattcgga tattgtgatg gatgtgcgtt
    22201 ttttgcccaa ccctcattat atcccggaac taagaccatt gaccggaaaa gatcagccag
    22261 tctatgatta tgtgatgtcc tttgatgaaa cgcaatcatt ctaccataaa tttttggatc
    22321 ttctggaaac tgtattgcct ggatacatta aagaaggaaa aagcagcttg acagttgcaa
    22381 tcggctgtac aggcggtcaa caccgttctg tggcattgac tgaacgtttg gcaaatgctt
    22441 tagggaaaaa ctataaagtc aatgtgacac atcgggacaa ggataaaagg aaagagacgg
    22501 tgaaccggtc gtgaaaatga aaacctaccg gataagaaaa ccaaagatcg tagtggtcgg
    22561 cggtggcact ggtctgccag ttatcctgaa aagtttgcga aatcaaggag cagatatcac
    22621 agcagtagtg actgttgcag atgatggggg aagcagcggt gcaatcagag aatcgatcgc
    22681 tatggctcct cctggtgact tgagaaatgt cttagtggcg ttatcggata tgccacaatt
    22741 ctatgaagat attttccaat accgtttcaa aaaggaagat cagtttttag ccaatcatac
    22801 gatcggtaat ctgatcattg ccgcagtttc cgagatgcgg ggaagcactt atgaggcgat
    22861 tcaattatta gcaaaaatga tgcatgtaga tggacatgtt tatccttctt cagaaactcc
    22921 tttgaccttg catgctgtct ttaaagacgg aacgactgca gtaggagaat ctaaaatcgc
    22981 gatcgaccgt aaaaccattg accgtgtgta tgtctgcaac acaaatgata aaagtgaagc
    23041 caaagcggct cgaaaagtcg tttcagcaat tatggacgcc gacatggtcg ttttgggacc
    23101 cggaagttta ttcaccagta tccttccaaa tttagtgata ccagaaatcg gcgaagcaat
    23161 gctcaagaca actgctgaaa ccgtctatat ctgtaatatc atgacccaaa aaggggaaac
    23221 ggaacatttc acagatgccg atcatatacg tgttttacac aagcatttga atcagccttt
    23281 tatcgataca gtattggtca atacggaaaa agtgcctgaa gattatatgg acccagagat
    23341 ctatgatgag tatctagtac aagtcgcaca cgacttcaac ggattgagaa atgaaggatg
    23401 tcgagtgatt tccaccgact ttttggaatt gaaagatggt ggtgtcttcc acgatggaga
    23461 caaagtagta gaagaattgt tccgtttagt tttcggttca aaatattaag caatttattg
    23521 ttggagaaag gaggagaata ggatgtcatt tgctgcagat gtgaagaaag aactaactgg
    23581 tttggaagtt catcgagaac atgcgaaagc ggaattagct gctttgctgc gaatgaacgg
    23641 ctctttaggt attgtgaacc agcagtttat tttgaacgtc caaacagaaa atgctgcaat
    23701 tgccagacgt atctattctc ttttgaaaga tcattatcag gtacgaagtg agctactcgt
    23761 tcgtaaaaaa atgaaattaa aaaagaacaa tgtctatatc gtccggttga agcaaggcac
    23821 aaaagatgtg ttgatggatc ttgatatcat ggatggtatg atgttcaatg gacacgtttc
    23881 ggacgagatt atgggaaatt cgcaaaaaat gcgttcttac ttacgtggtg cttttatggc
    23941 ctcaggttca gtcaataatc cggaaacgag ccgttatcat ttggaaatat tttctattta
    24001 tgaacagcat aaccaagaca tttgcgatat gttaaattat tacggtctac atgctcgaac
    24061 gcttgaacgg cgcaatggct atatttctta tttaaaagga gcagaaaaaa ttgctgattt
    24121 cttaacattg attggtgcaa ccaactcaat gctccgattt gaagatgttc gaatcgtacg
    24181 ggatatgaga aattcggtca atcgtctagt caattgtgag acagcaaatc tgaacaagac
    24241 gattgatgcg gcttctaaac aaatcgaaaa catcgaattg atcgaagcaa aggtcggtct
    24301 acatgccctt cctgaaaaac ttcaggaaat cgcagaactt cggcttcaac atccagaggt
    24361 cagcttgaaa gaattagggg aaatgattcc ctcaggtgcg atatcaaaat cgggaattaa
    24421 tcaccgaatt cgcaaaataa atgaatttgc ggaaaaactg cgtgaagaag cagtttaatc
    24481 caaagaaaaa actgatacat gaagcaggag atgcgaatgt atcagttttt tttaaaagtt
    24541 atacttgctt ttcttccgtc aaactataat tccatcttta ctggagtaac ataatttgga
    24601 aaggcttcta ttgtttttaa tacaatcgta taaaaagcgg ggagacaact gaatgaagat
    24661 tttagttttg tacataaatt tttgcttaat atagaagtcc tgatatagat aagatcaagt
    24721 atttatctat aactggcctg attaattaaa aaactggaag gaactgctcg atcatccttg
    24781 agaactgctc ttcttacttc tggtcaagat tctcgtggtt ggtggataag gatggttgat
    24841 aagttcattg gattagctag tcgaaaacaa ccaggtgtaa ttaatgtaga agttttaaca
    24901 aggggatgga gtgatttatt cagtgataat cataagttca taaatagtaa ttgagatgta
    24961 tggttggaat acgttatacc tgaacttgaa ctaccaagat ttggtttaga atttgagaat
    25021 cgatgtttat aaagggaggg caaaaatgcg tttttttaca acttttacta tcattatgat
    25081 tgcagtactt tttatttttc tagatatagc aaagcgaaat acagcatttt tattatatcg
    25141 ggttttactt cgagcaggtc tcataacatt tatatcgatc gttggatttt ttctattcac
    25201 agttattgta tttatttgga gaactccagc accaccattg ccagagatta catatggaga
    25261 atttcctttt agacttgagt atgaattgaa tgaagaatta catgtgatag aagacacgtt
    25321 aatagttgaa tttgatggat ttggaatgaa cgaaggcata gggagatatc gtagatggac
    25381 aagtcgttta gccagcggag aagatttagt gttgctgtta gaagttagtg ataataagca
    25441 aatattttat tttccaggac ctgctaatta ttacatggga gataggttga atggttataa
    25501 tcatactttt cctagtgctt ctttcattga aagggaaaga ggcattacta gaagggatat
    25561 tttacatgac aaagaattat tagaacaatt tggtccgctt gatcaaaata caataaatga
    25621 agaagaattg ttaaatcaat ataatataag gctagtgaat tgggaaatat ctgaaccgat
    25681 tgttaacaat tttggtgatt agaaaacaat tacgataaat agaaacaagc caaatcatca
    25741 gcaagtaaaa gctgatgatt tggcctttct tatacgagta gtaggaattc atagcgaata
    25801 ttattacgat cagtagatat tttgatatta aaggagtatt acgaactagt atgtgagaat
    25861 gtagaggtga tttgcgaata atgagaaatt tatcaaatag aaacaaaata ttaatcatta
    25921 ttgttgttat agctgtgttt catctgggaa cgaatgcggt tttaagtaga ataatattgg
    25981 gtcctaagcc accaagacct gcgattaccc gaggggagtt tgattttaga ttagaatatg
    26041 aagtagatgg ggaaaggatt gtgatagaag atacaattgt tgcattattt gatggatttt
    26101 ctgcagatgc aggtagcatg gcatggtatc gcacgtggcg tttgcatttg gcgagtgaca
    26161 agagggcaca acaattattg ttacatgagt tagatgatgg tcgaaaaata tattatcatt
    26221 ttggaggagc tggattttat atggggaagt tagtagcagt gtaactgtaa caaggaaaga
    26281 tttgccaggt cttggcattc gaaactccat attttatatc gagcgtggga gagatacatc
    26341 tattgggaac aggaaagggt taactcttga agagttatat aatgactttg gaatccgtct
    26401 tgttagttgg gaacacaatc caccaataga aaatcgcttt gaataatcga aatctattaa
    26461 tccatttcat cagccagtga atgttgtaat ggaacaattt ggtttagtgt tcaaaaccat
    26521 tgaaatacta ggctgtgatc agcaaaaatg tatctgaata aaaaagagag caactattaa
    26581 atgagaggag ttaggagttg ttagctttat acgaatcaaa gtggcaaata cagatgcgta
    26641 tgcatttgtg tttgccatct tgcgtttggt agacgagata ggtataagct aaagaaacaa
    26701 cttgtttaaa ataaacgacc tagctcaaca aacgaccatc taatcaacta acagcataca
    26761 taaaagcaac tatttttgaa gtagaattga aatcgggata acttaaaaag tgattgggat
    26821 actagtgtga atcaccgtac ataaatatta ctcgttaagg gaatttgaga aattttgtat
    26881 gaaaatttaa gaattagtta gtatgcgcaa gtcaaaaata tgaataaatt atttggtgga
    26941 gataatttgc tcagcttgtc ataagaaatg agataatctg gcagatgcaa gcttttggac
    27001 accatttatt cggaataa
//
LOCUS       DANKHL010000012        26428 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000012 DANKHL010000000
VERSION     DANKHL010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.014"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            195..2030
                     /gene="tdc"
                     /locus_tag="PW101_000370"
     CDS             195..2030
                     /gene="tdc"
                     /locus_tag="PW101_000370"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL2512801.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2335..3813
                     /locus_tag="PW101_000371"
     CDS             2335..3813
                     /locus_tag="PW101_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2512802.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3779..5110
                     /locus_tag="PW101_000372"
     CDS             3779..5110
                     /locus_tag="PW101_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2512803.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5224..7776
                     /locus_tag="PW101_000373"
     CDS             5224..7776
                     /locus_tag="PW101_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDL2512804.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7783..9222)
                     /locus_tag="PW101_000374"
     CDS             complement(7783..9222)
                     /locus_tag="PW101_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2512805.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9568..9930
                     /locus_tag="PW101_000375"
     CDS             9568..9930
                     /locus_tag="PW101_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDL2512806.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10117..10974)
                     /locus_tag="PW101_000376"
     CDS             complement(10117..10974)
                     /locus_tag="PW101_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2512807.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11094..12005
                     /locus_tag="PW101_000377"
     CDS             11094..12005
                     /locus_tag="PW101_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL2512808.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12012..13658
                     /gene="mdcA"
                     /locus_tag="PW101_000378"
     CDS             12012..13658
                     /gene="mdcA"
                     /locus_tag="PW101_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HDL2512809.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13760..14059
                     /locus_tag="PW101_000379"
     CDS             13760..14059
                     /locus_tag="PW101_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HDL2512810.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14049..15725
                     /gene="mdcD"
                     /locus_tag="PW101_000380"
     CDS             14049..15725
                     /gene="mdcD"
                     /locus_tag="PW101_000380"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HDL2512811.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15729..16322
                     /locus_tag="PW101_000381"
     CDS             15729..16322
                     /locus_tag="PW101_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HDL2512812.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16346..17422
                     /locus_tag="PW101_000382"
     CDS             16346..17422
                     /locus_tag="PW101_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDL2512813.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17447..18316
                     /gene="mdcB"
                     /locus_tag="PW101_000383"
     CDS             17447..18316
                     /gene="mdcB"
                     /locus_tag="PW101_000383"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HDL2512814.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18427..19191
                     /locus_tag="PW101_000384"
     CDS             18427..19191
                     /locus_tag="PW101_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HDL2512815.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19680..21773
                     /locus_tag="PW101_000385"
     CDS             19680..21773
                     /locus_tag="PW101_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2512816.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21798..22100
                     /locus_tag="PW101_000386"
     CDS             21798..22100
                     /locus_tag="PW101_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2512817.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            22127..23416
                     /locus_tag="PW101_000387"
     CDS             22127..23416
                     /locus_tag="PW101_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2512818.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            23435..23842
                     /locus_tag="PW101_000388"
     CDS             23435..23842
                     /locus_tag="PW101_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL2512819.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            23865..24734
                     /locus_tag="PW101_000389"
     CDS             23865..24734
                     /locus_tag="PW101_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HDL2512820.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            24736..25356
                     /locus_tag="PW101_000390"
     CDS             24736..25356
                     /locus_tag="PW101_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2512821.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            25806..26411
                     /locus_tag="PW101_000391"
     CDS             25806..26411
                     /locus_tag="PW101_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2512822.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
BASE COUNT         8590 a         4519 c         5654 g         7665 t
ORIGIN      
        1 attgaaaaat tgttcgcttt tttgcgttga attatcagaa aaatccgact ttatttattt
       61 ttgtttgtaa aaatctacgt ggatttgatg ttataaaaaa aaaattctca tttttggcgc
      121 tatagtgaag aggtgttaca gataaacaca tgtatctgca agacattgac caacaaaata
      181 tggagggaag aaaaatgaaa gatatggata tcaaggccgt ctttataggc gacaaagctg
      241 aaaatggacc agtttataaa atgttgttga acaagatggt agatgaacat cttggatgga
      301 gagaaaatta tattccttca gatatgcctg caattagcga aggagataag ctcactccag
      361 attatctagc cacccgcgac catatgatag aggtcttgga tgaagtcagt cagcgattac
      421 gcgcaggatc gatcccatgg cattcagcag gccgttactg gggacaaatg aatgctgaaa
      481 cattgatgcc tgctttatta gcttacaact atgcgatgct gtggaaccca aataatgttg
      541 cattggaatc ttctatggca acttcgcaaa tggaagcaga agtaggacag gattttgctt
      601 cattattcaa tatgacagac ggatggggac acattgcagc agatggttct attgctaatc
      661 ttgaaggatt atggtacgcc cgttgcatca aatcgattcc tttagctgtc aaggaagtgc
      721 tgcctgaaaa agtgaaaaaa atgtctgagt gggaactttt gaatttatct gttgaagaaa
      781 ttttggaaat gaccgaaagc tttacggacg aagaaatgga tgaggtcaaa gcagcttctt
      841 cccgtagcgg aaaaaatatc caaagattag ggaaatggct ggtccctcaa acaaaacact
      901 attcttggat gaaggcgtta gatatttgcg gagtcggctt agatcagatg gttgctatcc
      961 cagttcaaga ggattatcga atggatatta atgctttgga aaaaacaatt cgtgaattag
     1021 ctggacaaaa aatcccaatt ctaggtgtgg ttgctgtcgt aggaacgaca gaagaaggtc
     1081 aagtagacag tgtagataaa atcgtccaat taagagaaag gttaaaagat gaagggatct
     1141 atttctattt acatgttgat gccgcatatg gcggctatgc gcgttcactg tttctaaacg
     1201 aagcaggaga atttgtgccg tatgcttcct tagctgaatt ctttgaagaa catcatgttt
     1261 tccaccactg cgtaacgatt gacaaagagg tatatgaagg gttcagagct atttcagaag
     1321 cggattctgt cacgatagat cctcataaga tgggctatgt cccttatgct gctggaggaa
     1381 tcgtaatcaa acataaaaat atgagaaata tcatttctta tttcgcgcca tatgtgtttg
     1441 aaaaatcagt aaaggctcca gatatgttag gagcttatat tttggaagga tcaaaagctg
     1501 gagcaaccgc cgccgcagtc tggacagcac atcgtgtatt gccattgaat gtgaccggat
     1561 atggtcaatt gattggggct tcaatcgagg cagctcaaag attcagagaa tttctcgatc
     1621 atctgacttt tactgttaaa ggaaaaacaa tcgaagtcta tccattgaat catcctgatt
     1681 ttaatatggt caattgggta ttcaaagaac aaggctgtac agatttgaac gctatcaatg
     1741 aattaaatga aaaaatgttc gatcggtctt cttatatgga tggcgatgtt tatggtgaac
     1801 ggtttattac ttctcatact acatttacac aagaagatta cggtgattct ccgattcgtt
     1861 ttgttgaaag aatgggtcta acgaaagaag aatggaaaaa agaacagaaa atcactctgc
     1921 tacgtgcggc tatcatgact ccttatttga atgatgatcg aattttcaat ttttatacaa
     1981 aaaaaatcgc caaagcaatg gaaaagaaac taaacgaaat catccaatag aactgatttt
     2041 tgtgtgaaag aataacaaga gacatatgga gaacagacaa ttatttgctt catcaaaaca
     2101 attgtatttc cccctgtttt tcatatattc ctcgccaaga tattcttttt tataaaacgt
     2161 tctataagtt ggatcgttcg tttcatgtat caaacccagt tcactaagat cgttcaatgg
     2221 atgtttcggt gatactgggt tttgtactga atcgatgaaa ccttgtcatt tctgttcttg
     2281 aacaaaaata aaagaaaaca aaaacatatg tcgctattga aagaaggaat aaagatgaaa
     2341 gatactggtt catcaaagca aatcacgacc atgaccttta tcggaatgac ctgcgccctt
     2401 gtagcaagtg tacgaaatat tccagatgta gcagcaacga gctggacgat gattttttac
     2461 atgctggttg cagtattatt gtacgctttt ccaatctcat tgatctctgg tgaatttgct
     2521 ggaatgtttc cccaaaaagg tggtccagaa ctttgggtat cgaatgctct aggaaaaaaa
     2581 tgggggttcg ttgtctcatg gctgttatgg gtccaaatgt ttcctgggat ggtgatggtc
     2641 gcttcagcac tcgctccgtt gtttggtaat attatcgata atgtgccgct tggtttgaac
     2701 agcaaattta cattagttgt cattttagtc gtttattgga tcatcacttt tttgaatttg
     2761 aaatttgata tggcaaaaat cggcggaaaa gtcggtgttt ggttaggatt gtacgtgcct
     2821 ttgacgatga tgctcttatt agggtttgct gcgtggatca aagttgggat cgtaccaacc
     2881 ggaacattag gcagtttttc atgggacaaa ttaataccag atactacgac tgcatcctca
     2941 tttgtttatt ttgcgccgat catgtttatt tttactggta tcgaaatgtc ctctgtgtat
     3001 atcactcgat tggaaaatcc agttaaaacg tatattcgcg gagtatttgc cgcgttaatt
     3061 tttatatttt tcgtcaatat tttgaatgct ttagttgtag caaatgtcgt accaaaagga
     3121 caaatggaat tgaataatat cgcgcaatct atttcgattt attgccaaat tcttggtttg
     3181 ccacatctta ttgtgaatct gtttagttta cttgtcttca ttggtgtagc agtccaatta
     3241 tctgcttggg cctcaggacc agcaaagaca gtgacagaaa gtgctagaaa aggtgcgtat
     3301 ccaccaaaat tcaatttttg gaagacgaat caatttgacg tatccaaatc cgttattttg
     3361 actcagtctg tcattatctc aatctttgca cttttttatt tgctgattcc tggcgtcaat
     3421 caagcatttt tgatgctcgt aaattcaact actgttatct actgtatcgt ctatgtcatc
     3481 atggggatcg cagttttgag attgcgttat acacatgcaa agttgaatcg accatttaga
     3541 attggaaaaa aagagaaaaa gagcaatctt ggagtttgga ctgtcgttat tgttttattt
     3601 gcggcaatcg gattttctgt cggactgacg atgaaagctg gaacatggat caatctaatt
     3661 gcagtcacag ccatcagtgt cattttgttt gtagtaccac tctgtattga aaaaataaag
     3721 aaaccttctt gggaacagga agtcgccatg ctgcttgaaa aagaaaaagg tgaacttgat
     3781 ggaaaaaccg gcaatgaaac ggactctaac taatcgaaat atccagttaa tcgcattagg
     3841 tggcgcaatt ggaacaggat tatttttagg ttcagcaaag gcaattgcat cagcagggcc
     3901 agctgttatt ctcgtttatt tgatatgcgg tttggctatt tatggtttga tgcgggccat
     3961 gggtgattta tttttagcaa atagtacatt taactctatc agtgatttcg ttgactatta
     4021 tttaggcaaa aagtgggcat tttttgtcaa ttggacttat tggctttgct ggcttggagc
     4081 tggacttgcg gaattaacag caataggatt gtatatccat ttctggtttg tacatgtacc
     4141 agctttgata agcggaggta gcgcactaat gatcttaatg gcagtcaacc ttgttgcagt
     4201 caagtggttt ggtgaattgg aatctttttt ctcaatcatc aagatcttgg ggattttcct
     4261 ttttatctta ttgggtatag gattgagtgt ctcagatttt catacccagt ctatcggaat
     4321 caaagaaaat tttggacaga tcgatgaatt actaccgttt ggctttgctg gaatggtagt
     4381 ctcatttcct atggttttat tcagttttgc tggaattgaa atgatcggct taaccgttgg
     4441 cgagacaagt gatccgcaaa agaatttgaa aaaagcaatc aactgtctgc cttggcgtat
     4501 tctctttttc tatataggaa cgattttgtc gattttattt gttgttccat ggcattgttt
     4561 agatttaaaa gaaagtcctt tagtgactat gctgcaagtg atccattgcc aaggcggagc
     4621 agcaattgtc aatctaatga ttttggttgc ttctttgtcc tcagcaaata gtgcagtcta
     4681 cagtactagc aggattttat atcaaacagc aaaagaaaaa agtttgcccc attggttttg
     4741 tgcgctttct aagaaaggcg tacctttaca cggtatttta attacaggaa tgttattttt
     4801 tgccggtctt tgtctacatt tttttgtagc tgatagtcga ttgttttttc agcttttggc
     4861 aggcatgaca acgagttgtt ttctatttgt gtggtcttcg atcttgtgtg cacatgttca
     4921 atttattaaa gaaaagaaac ggaacactac acaaagatat aaagatcttg ctttgttggt
     4981 gttttttact tcaattgctt tcagcttgtt attcgaaagc acgacacgct ttattccttt
     5041 tttattggtt atctggtttg tattcttaaa ttttgtatat aaaagaataa gcaatagaaa
     5101 tatcagttag gcccagatac attttgcaaa acgaattatt tcagaccccc ctgaaataat
     5161 cgatacgcct gaaaaaactt ccctgcgtat aataacagta tcaattaaac aggaggggat
     5221 aggatgaact ggtacagatt gacggtagaa gatgtattga aaaactatca aacatcaaca
     5281 gaaggcttgt cgcaagaaga acgacagaaa cgcctgactg atcaaggata taatgaaata
     5341 aaagcaaggc agcagacaaa acgatggaaa aaaatagcaa agcattttac tgatctgctg
     5401 atgatcgtgt tgatcattgc ctcttttttg aaatttgctt ctagtgaata tattgaagga
     5461 agtattattt tattcgtagt gatcgtcaat ggactcgtta gttactggca agaaagaaaa
     5521 gcggaagaat cattgaatgg tttgaagcag ctgatgggac aagaagcgat agtactatca
     5581 aacgggatgc aagaaaaaat ccctgcgaaa gaactggtaa aaggagatgt cattgtctta
     5641 cgtgccggag atgtcgttcc agcagatatc cgattaatcg aagcacatga tttattagtg
     5701 gaagaatcga ttttgacggg agaatcagaa gcatctgaaa aaagtcatgc cgttttgaca
     5761 gaagaagaat cgatcggcga ccagaaaaat atgggttttt caggaacgct tgtacaatct
     5821 ggttctgcag tcggtgtcgt tgtagaaaca ggaaacacga cagaaatcgg taaaatcaat
     5881 caagcattgc aatcggtcga acaacaaacg acgcctctga ttaaaaaaat caaccgatta
     5941 aacaaacaaa tttttcaagg cattctttgt ttgagcctat tcttagttat ttttacgaca
     6001 tttcgatatg gtatggattg gcatattctt ctttcggcta cgattgcttt ggtggtgtca
     6061 atggtacccg aaggattgcc agctgttttg accatgattc tatccgttgg agtacatgaa
     6121 atggcaaaag aaaaggcgat catcaaagga cttccttcgg tagaaacatt aggttctatg
     6181 acagtgatct gttcagataa gacaggaaca ttgacaaaaa atgaaatgac agttgtggat
     6241 gtagctgcaa aagaagaagc ctgcgtgttg tccattatga aaaactgtca ggaattaaaa
     6301 acaaaagaag aacagaaaac agaaaattta agtggcaatc cgacagaagt tgcgctgatt
     6361 cgacacacag aaacagccaa tctacctttg aaaaaaatag aagcaaagat tccgttcagt
     6421 tctgaataca aatatatggc aacgatgcat gcggaggaaa aaggagcggt gatttacgtc
     6481 aaaggtgcac cagaagtatt atttgctaaa tcaactcttt cttctgcaga acaagaagca
     6541 tggagtcaga cagctgcaga atttgcgaga aaaggtcagc gtgttttagg atttgcttat
     6601 aaaaaagtag attcgaaaca ggaactcacg catgagactt taacccaatt aacttttgca
     6661 gggatcgccg gattgatcga tccgccaaaa gaaagtgcag tcaaagctgt aaaggaatgc
     6721 caacaagcag ggatctcagt gaaaatgatt acaggggatc acaaagacac agcaaaagcg
     6781 attgcggaac aaattggctt aaaacatact gccaaagttt tagaagggat cgatttggat
     6841 cttatgtctg atgaagagtt gattcagcaa gtgccaatag tcgatgtctt tgctcgaacg
     6901 acaccagaac ataaactgcg aattgttaaa gctttgcaga aaaatggaga aatcgtcgga
     6961 atgaccggag acggcgtaaa tgatgcacct gctttgaaaa gatccgatgt aggaattgct
     7021 atgggaatca aaggtagtga agtaagcaaa caggcagctg atatggtttt aggagatgat
     7081 aatttccata cgattgcaaa agccgtaaaa gagggccggc ggattttgga taatttgcaa
     7141 aaaaccatca atttcttctt gccaactgca ttagctcaag gattgatctt gatttgggca
     7201 cttatgctga atcgcccgct acctttgtcc ccagtacaga ttttatgggt caatatggta
     7261 acgacaatca ctttatctta tgcactaggg ttcgagtcgc cagatccaga aatcatgaag
     7321 cgcccaccaa gagatccaaa gcaaggaatc ctatctggtt atcatttgtt tcggatcatc
     7381 tatgtctccc tactgatcat ggttcctgcc tatttattgg ctatgcagtt tgaagggcaa
     7441 agcttacagc aaaccattct tctgcaaaat atcgtcttag ctcaagctgt ttacatgatc
     7501 aattgcagag agttgtccaa gccgtcattg aaccaaggtc ttttgcaaaa caaagcgctt
     7561 tttctttcat taggtatcct tgctttattg caagggatgg ttatttttct accagtcggc
     7621 cagcagctga tcggtacagt aagtttaacg atgacacaac agttattgat tggagcaaat
     7681 gtgattcttc tattcttagt tgtagaaata gaaaaaggga tcatgaacaa actgtttcgc
     7741 aaaaaagaaa aaacagctag agaatacagt cgctagccgt tactataaaa aagttttgaa
     7801 agtgagttca gtcaaacgat tgagctcatt tttcgttgga atagaagaaa tgtatacaat
     7861 agggatatct agagaatcta gagggaaatt ggaaatcaag atatctgttt tttcaaaaga
     7921 aatagtggaa agatcggtta gttcgatagg taagaagcag acacgttttc ctaagtaatc
     7981 ttttaattcc tgctgcaaga acgccttcca tgaaggttct ccttggaaaa taaaatagac
     8041 attgaacaaa ttcggtttga tagaaaaaat cgtttgttgg atcagtagag acaagagttg
     8101 gagaaagtaa tttggttcat tgatttctat ttcttttttt agatcgttca gtaaattctc
     8161 tttctgttga tacaagtgtg catattgctg atcatatgtt tctaaaaaac gatggtattg
     8221 acggtccaat gcttcaatca atatcggcga ttcatgatat tttaatatga ggagagccaa
     8281 gttatcagtc agagatgttt tttttagttg atcaagagac agctctgaag ctagttcctc
     8341 tacaaacgtt tcgaggacct ctaaaaatgg agaatgatgc aggcagcgag cggtttcttg
     8401 tccatagtta ttgttgtaat tccaactttc cagaaagcag aaagaagcaa aagaaagctc
     8461 agtgtcttta agataaatac cttccgttaa atacaatttt ttcaatgcag agtcatacag
     8521 ggaggagagt gtgctgttgg tatggataga agaatctaat cggcaattcg cttttatacg
     8581 gatcgtattg ataaagaacc aacatgttcc gaaaatctct tctgtttcaa ccgctagttc
     8641 agtttgactg aggtttctta aaaattgaaa ataatgggat tcatggatcg aataatcctc
     8701 gaaaaaatag ttgttatgag tgaaaggaat cagcaatcga tgataaaaag tacgaatcgc
     8761 acgctctttt ccaagaatac aagctgtata tttcgtcaaa tcgatctgca agtcgtaact
     8821 tgctaaacgc tgattgattt tagtcacatg tcttttcaat gtcccataag aaagcccatg
     8881 gtcggctaga aaatcaggga cagcaacttt ttttgtaaaa agcagatttt tcagcagttc
     8941 gacactgact gattgtttga aaaaaatctc ccaaaggtca tttgttaata aattttgctg
     9001 gttttgcaaa ctgattcctg cttgatcgct gatgatgtcc cagccttcgg gcagctgaga
     9061 gcgaatagat tgaaggtcag aaaaaatcgt tcgttcggtt gtccctatga tttcagctaa
     9121 ttcatttacg gttatctttg tatgattcag caattgttct actaaagaaa catgacgtcg
     9181 aatatctttt tcggtaatga tttttttcat tagtgaatac atcaatcaga ctcccttcta
     9241 ttctaagaat acggagaaaa taaacggtgg tcaagtttca actgattttt caatcattgt
     9301 ttatttttct ggagcattta ctgatgataa gaatttcatc atgaacttga aaaatgatac
     9361 cactaaaatc aaaaaatagg tgggcaaaca ataataaaca ttttaatagt agatggtagc
     9421 aagaccaaca caggcggatt gaacatttaa gagttgataa aaaagaaaaa tatcgaagta
     9481 tttgattagc aactacttgt attcattttt ctattcttac gtggtatctt tgaataaagc
     9541 gaatcaataa gaaagagatg gaagcagatg ctaacgaaaa aagaactaat cgatcacatc
     9601 aatgaaacat acaatttgtc tccagaatat atttttaaaa aatttcctga ttattgcgta
     9661 ttcagacatc aaaaaagcaa aaaatggttt ggggtcctga tgtctgttcc agaagaaaaa
     9721 atctatgatg atgcaagtga ccataggatc atcaatattc ttgatttgaa ggtcgatcct
     9781 gaacttggcg gaattttgcg aggaaaagag gggtattttc cagcctatca catgaataaa
     9841 gaacattgga tctctattga tctacgtaaa atagaaaagc tagatgaact agcagatttg
     9901 attgatggaa gttttaaatc aacgcaatga atgtctagga caatagagaa gaggttgtga
     9961 cagaaatgtt tagcttcaag aaataagaag gaatttacga aaattgcttc tcaaattttt
    10021 gggaaatttc ggcttatttt ctaagaaatt acttctggaa caccgtttgt tcgaaaaaag
    10081 agctgcgaca agaattttgt cccagctctt ttttttttat tttcctaaat aaaattcccg
    10141 catcgttttt gcaatcattt tgatcaaagg atgatcttct gtgaagctag aagataaata
    10201 aattgttcgt gtcaagggca aatctttgat ttcccgcaag acgaaaggat catttttatc
    10261 gtaagaccaa gaaacttttg ggaaaaagct aacagctaat ccttgcttga caatagaacg
    10321 taatgtcgca ggatcatctg tcgtcgaact gatcgtcaat tcgatacttt tcgcttcaag
    10381 tgctgcatcc agtgtatgac ggaaggcgtt gctctttgac agcatcaaga tgggggtgcg
    10441 ttggatatct tctaattgca gatgttcttt tttagcaaga ggatgtttct taggaatggc
    10501 gacataaaca ggttctttca tgataggaat attggtcatg ccaggcttca tctccgaagt
    10561 gatgtacagt acaggatcgg taatatcatc tggtaaatga tgttgcatga tcgttaattg
    10621 gatatcagga tgcttttcca taaaaattcg gaccaagttc ggaatagaaa cagaggctac
    10681 ctctaaatac aatatgacgg aagtaacgat cttctcattg accgaagaca ataatttgat
    10741 tccttcttct aatgtgtcta atgtcttgac tgcatatgga taaaattctt ctgcataaac
    10801 ggtcgtctca atgtttctgc cgctatggta aaacaaaggg acacctaatt cgttttccaa
    10861 atgtttgatc gcacggctca aggcaggttg agaaataaat aattctttgg ctgcttgggt
    10921 aacatttttt gtttcgaata cttttttaaa atactgcaga tgaagcaatt tcataattat
    10981 ccatcccatc catacctttt tgttatggtt ttttcttttt ttggcatttt ttattatagc
    11041 agaaattgac taagatagca gtggaaaaag taagtgctga ggaggcgagg ctcatgagta
    11101 caggtttctt atttcctgga caaggcagtc agaagttagg gatgttagcg gatatccctg
    11161 aaatgtacat ggaacgttta gaaaatgtga cggggtatcg gttagttgat aaagaagaaa
    11221 actatcaaga tacggttttt atccagctag cattactgac aaaagcagta ttttatttag
    11281 atgaaatgga aaaaaacggt attcagcctg atattgcagc aggacattcg attggtgctt
    11341 tttgtgcagc tgttgcttgt cgatctttga gatttgagaa tgcagcggaa ctagtctatc
    11401 accgcgcagt cttaatgaaa gaagcttatc caagcggtta tgcaatgggc gtagtagtag
    11461 gtgtgacgag aagcgaagct gaggaaattg tagcaaaaac atttgatcct gaccatccag
    11521 tttatctgtc aaatgaaaat tgtccgatgc aacatacgtt atctgggagt atcgaaggtt
    11581 tatccgttac gttggcagaa gccaaacggc accaagcgca aacagcgaag ctgctgaaag
    11641 tccctgtgcc atctcactgt ctcttgatga atgatacggt caagaaattc aagccgtata
    11701 tgcagtctgt ccatctatct gatgctagat gtttgtatat aaaaaatacg gatggaagag
    11761 ccactcagga cgcagaagag attcgtaaag atttgttgga aaacctagct ttccctgtgc
    11821 agtggaacca aatgatggac ttggcaaaag aattaggaat ggatctgacg attgaatttc
    11881 cccccggcaa tactttgaca aagctgattc atacaaaatt tggtgtagat aaagggatcc
    11941 gtacgatcaa tctagaccaa catggtattg atgacgccac gtttctatat caaaaatgga
    12001 gatgactaat tatggaaaaa ccaaaccgca actgggcttc taaacgcgaa aaaaaagcgc
    12061 agcgattgga aaaaatcagc aacttgctca atggcaagta tgtagaaact gaaaaaatcg
    12121 tagaagtaat ggaaaaactg attgctcccg gcgacaaagt tgtattagaa ggcgacaatc
    12181 aaaaacaagc aagttttctt tcagaaagtc ttgaaaaagt agatgcagaa aaagtgaatg
    12241 atctgcattt gatcatgtct agtatctcac gaccagaaca tttgaatatt tttgaaaaag
    12301 gaatcgcaag caagatcgat ttctcttatg ctggtgcaca aagcttgcgt gtcgcccaaa
    12361 tgattgaaga cggcacaatg aaattaggtg atatccatac ttatttggaa ctttatggac
    12421 gcttattcat cgatttgatt ccaaatgttg ttttagtagc tgcagataaa gcagataaag
    12481 acggcaactt gtacacaggt ttcaatacgg aagaaacacc aacaatcatc gaagctgcag
    12541 ctttcaaaga tggtatcgtg atcgttcaag ttaacgaagt tgtcgattct ttaccacgtg
    12601 tagatatccc tggtgactgg gtagacgttg tcgtgaaagc agataaacct tatcaattag
    12661 aagctttatt cacacgagat cctcaaaaca tcacagaatt gcaaattcta atggcaatga
    12721 tggcaatcaa aggaatctac gcagaacacg gcgtacaatc tttgaatcac ggtattggat
    12781 tcaatacagc agctatcgaa ttgttactgc caacttatgg tgaatcacta gggttaaaag
    12841 ggaaaatcgc gaagaactgg gtattgaacc cacatccaac gatgattcca gccatcgaat
    12901 caggttgggt tgaaagcatc catagttttg gtggcgaagt tggtatggaa aaatatattg
    12961 ctgctcgccc agatgtcttt tttgtcggta aagacggcac gatgcgcagt aaccgaacat
    13021 tatcacaagc tgcaggacaa tatgccatcg atatgtttat cggttcaacc ttgcaattag
    13081 attacgaagg aaactcttct accgtaacaa aaggacgttt atctggtttt ggcggtgcgc
    13141 ctaacatggg acataatcct ggcggtcgtc gtcattctac accagcatgg caatcacttc
    13201 gcgacaatga tgatccatta gggaaaggcc aaaaattagt tgttcaaatg gttgaaacat
    13261 atggttcaaa taaaaaacca gtctttgtcg aagaattaga tgcactagct gttcaaaaac
    13321 aagcaggctt agccaatgca ccagtgatga tttatagcga agatactaca catatcgtga
    13381 cagaagaagg aattgcttat ttgtacaaag caaaaacaaa agaagaacga caagcagcaa
    13441 ttgcagcaat tggcggtgtt acacctcttg gaatgactag cacgaaagaa tcactagata
    13501 gcttgcgtgc agccggcatc gtgaaattac cagaagatct aggcatcaaa cgtagtgaag
    13561 cgaaacgttc attattagct gcacaaacca tcgatgattt gatcgaatgg tcagatggcc
    13621 tgtatgaacc accaatgaaa ttcagaactt ggtcataaaa ttggcgagaa aaaccttttg
    13681 tcgccattta cccagatgga agaagaaaga cgaataagca gaacgtgtat gctctgtttt
    13741 acaaaaaggg gagagatcca tggaacaatt agcttataaa tatccaacga aacagccgat
    13801 caataagaaa atccatgtag gagtggtcgg ttcaggagat ttggaaatct tgatgtttcc
    13861 aacagaaaaa acagaatcta ctgtaaaaat ttgtacagga agcgatggct tcggcgaagt
    13921 atggaacaat gtattaacga gattttttga tcgttatcca atttcagcag atatcgtgat
    13981 caatgacttc ggtgctacac caggagttgt gtacctacga ttgacgcaag caatggagga
    14041 actttcagat gaaaaatagt tttgtagaat taaacgccag agaacgagtg aacgctctac
    14101 ttgacaatgg acgtgaattg attgatccat tcgatcaaat catcgcgcca agtttaccag
    14161 cacaaggaat cgtccctgaa agtgatgacg ggatcgttgt cagccgtggc acgatcaatg
    14221 gcaaacaagc agtagtaatc gctatggaag gaaaattcca aggtggcggt atcggtgaag
    14281 tttccggtgc aaaaatcgtt gctgcacttg aacatgtgtt agaagaaaat aaaaaaggaa
    14341 atgagatctt tccagtcatt gtactagata caggtggtgt tcgtttgcaa gaagcaaact
    14401 acggactgtt atctatttca gaaatcggaa atgtaatcat tgctctaaaa caatatgtac
    14461 cagtcatcgg tttgattcca ggacgtgtag gatcatttgg cggtatgtcg attacttcag
    14521 cattaatgag ttatttaatc gcaacaaaaa aagcgagagt cggtttgaat ggaccagaag
    14581 ttattgaaca agaagcaggc gttcgagaat ttgattctag tgacaaagac ttgatttgga
    14641 atacattagg atctcgtcaa cgggtagaat caaaaatcat tgatgaatta gtgactgata
    14701 gtgtagaagc aatcaaagct gctgtcatcg cagcaatgga cgaacgaaaa gacacgcatc
    14761 gcagtgaaag ctcagcattt tatttatcat tacttgattc tttagatctt tcaaaaccaa
    14821 tggccattga cgattacaat gaagcaattg ctaaccatca agcaaaatca attgacttac
    14881 cagaagtagc agaaggaaat gaaccagctg cagcaagtgt tggttacgat tggtttgcgg
    14941 cactaactgg catgaaaaat ccaacttctt ctattagtac tgtgtattat ggtaaatcaa
    15001 gtaaattcat agaagaagcc atcgtgacat caatcgtacc tgatcctaaa aacccgatgt
    15061 accgtgtacg aaacggtgaa gttggtttag ttgaaggttt ccgcatggga caaattttgg
    15121 atcacgttta cgaagaagat aaagacaaaa aagtgaaacg gccaatcatc gttgtgatcg
    15181 atgtaccaag ccaagcatac ggctataacg aagaattgat cggtatccat gtggctttgg
    15241 ctaatagtgc tgctgcttat gcaaaactcc gtcaagctgg acatccagtt attggattga
    15301 ttgcaggaaa tgctatttca ggtgcatttt tagcacatgg attacaatca agccgcttga
    15361 ttgctttaaa caataaagaa attaccgtcc aagcgatgtc taaagaaagt gctgcacgtg
    15421 tgacaaaacg ttcggttgct gaaatcgaag cagctgctga aaaagttcca gctatcgctt
    15481 acgatatcaa taattataca aaactaggag cgatctatca tttcctagaa gatatcaccg
    15541 accaagctgc ttcagaacaa aatgtcagca aagtcatcga agcaatcaat gaagccattc
    15601 aagatgtccg caatacaaat gacacgatgc tgacaaatcg atacacaaac gaaacagcca
    15661 aaacattcgg acgtgtcgaa acaaataaag tccgtgccaa aatggacgaa gaatgggatg
    15721 cataaaaaat gattcaagca catgatattg tcctttttga taaacatgaa atagaagggt
    15781 tcaccttgcc tgaatgggtc gttctaactg aaaagatgtt cggaacagtt cgtcgtgcgt
    15841 cgacggatca cgcagaaata ctaccgattg gtttacgtgg acctgcccgc aatcagagat
    15901 ggtcaacaga aatctcaaaa catgccgtca aacaagtcat tcatccgtgg gacatcatcg
    15961 aacaagaatc gtatagacaa gagaaaatca tggattatcc tgtttatcag caatttcatg
    16021 ctgctagaaa catattatca ggatgtaaat ggggaatcgg cggcagctta ggatttgaat
    16081 tatcgactgg gattccggca gtaaaagaaa ccagtgattt tgatttgctg ctgtacgcaa
    16141 attcaccgat tgaattgcca attcaggtaa ttcaatcgca tcctgctttt tttgagcaat
    16201 tcgataccca agtagtgact cggcaaggcg gtttttcact aaaagagtat ttgagagagc
    16261 cagagaaaaa aattctattg aaaacagtcg aaggacctaa attaaccaaa gaactttggt
    16321 aataaagaaa aagagagaga aagagatgtt tgaaaacaaa aaaatcaaaa tcaacgtaag
    16381 tgacttccag aaagtatcag ctgtaacggc tgttatgatg caaacgatat tgagctttgc
    16441 tttggcaaac agccacagcg caacaaatac agcttttctt ggtacctttt acgtcatcgt
    16501 taaatacaca gcaccaatgt ttatttttgc aattgtttat aatatggtaa aaacaagtca
    16561 gcatttgtct tattgggaat ttttgaaaga aaagtttttt gaactagtcg ttccgtatct
    16621 tctatggacg acggcttatc tttggctatt tccagcagtc caacaacaaa ctccgtatac
    16681 ggatactgca agctttttac tgaaatatgt cacaggtgat ggagcgcctc atttatggta
    16741 taccgttatg atgctgcaga tccaattgtt catgccgttt tttgtttggc tcggttacaa
    16801 agtatttgct aataaaaaat atgtatggcc agtattgatt ggttcaacga ttttatatgt
    16861 cgcatggtat ctgttctatc aaacacaagt attgactggg ccttatcatg acagctggta
    16921 cttattagat cggtttgttg ctagtttcat gatttatggg gtagccgcat ttgtttatca
    16981 cgaaaaagtc tatcaatatt tggacagagt acgttatctt ttcttacctg ttgccttagt
    17041 tatcgcgttc ttttctgtcc gttcattgct cgcacatcca ggagacttga gctttgcgaa
    17101 tgcaccttac ttgaatacga tccaaagttt atacagttta gtcatcatct ttgctgtctt
    17161 cataggcgct tccaaaatga ttgtaaacga ttcgccaaaa ttgccattgt tcaaatggtt
    17221 gtctgtttat gcttatcgta cgtatttagc caatgtattt gttttccaag tactgttatt
    17281 attgtttaaa gatagttggc tgcagttacc ttcaggagtc atgattcttg tagcttatct
    17341 aatgacggca agttgtgcat ttgctttaag ctggttactt catatcattt gggtagctat
    17401 caaaaaaggt ttcagcaagt aatagtagga agaaggagtg agtgtcatga taaatgacga
    17461 acgaaaaaaa gcagtgactc agcaactcgc tgcgttggta gaggaagcat tgatcgcaga
    17521 agtaacatta agtccaaaac ctggattagt ggatgcactg gatgcgggtg cacattccga
    17581 tatggactat gctttatttc ttaagtctgc taagacgcta actccttttt ttgaagaaat
    17641 ggctcaaata gcatggcatc atgctattga tcaggagtta agagaaagaa ttgctgagat
    17701 tgggcgaaaa gcggaaaagg caatgctgat tgctacaaat ggcatcaata cccataaagg
    17761 agcgatttgg gcaatggggc tattgatcag tgtcattgca caaaaaatca gtatggaaca
    17821 gcctttttct ttatcgaaaa tacttgatga tgtcagcaag cttgctgctt ttcctgatac
    17881 aaaatatcgt cataaaaaaa ccactcatgg tcatagggtg aagcaaaaat atggcgtaaa
    17941 tggtgcatat gaagaagcga tattagggta tccgcatatc aggcttgcaa ttcaagcagc
    18001 taaggagcta gataatgatt catatgtaca gaaacaaatg catatgttgt tagttttgat
    18061 gagttcgcta gatgacacat gcgtattgca tcgcagtgat cagaaaacgt tagtggaaat
    18121 gcaaaaactt gctcatgctg ctagtcaaag tgctttacca aatcgtccat ttacagcact
    18181 gttgcacttt tgtcaggaaa accaaatctc tcctggagga agcgcagatc tcttgtctgc
    18241 aagcttgttc atgctgaaga tagaggaatt atggactgcc gacacataca tttgtccgat
    18301 gttactcaat gactaaataa gacaaaattg ctaaaaaaac agtttatgca tctggttatt
    18361 gactcttcct tttagcaagg ctatcataaa aatagttttt tgtaataaaa gataacgagg
    18421 aagacaatga aaaaaatcaa ttatttagca atcattttgt tagtgggcct atgtttgttt
    18481 gctctgccga cccaaggatt cgctgtcgcg ggtggtcatg gtggaggtac atctagcggt
    18541 cgaagttcgg gcagaggttc atttggtggt cgttcatatg ggggcagtta ttttggcagt
    18601 cgttcttacg gcagagattc ttatgggtat tatggtccag gtacaggtcc ttctagttat
    18661 gagcaaacgg gatttcttgc attagctgtt ttgtcagtag gaggggtttc tgctttttct
    18721 atcgtcaaga ataaaagaca aaagaaaagg ctgaaagcgg agatattgtc ccgcatgtca
    18781 ggaacggcta aagaaaagaa agcacagttt ctggaaatcc aaaagatatt catgaagatc
    18841 caatccgctt gggaagaaga aactcctgaa aaagccagaa gctgttatac agacaagctg
    18901 tttgccgaac atcagaaagt tctgcaaaaa aacaaagcag acggtgtaag aaatcatact
    18961 aaaaaaataa ctattcagga agtaggaaat tatcgacaga tccacgaaaa tagttttgcc
    19021 atccgaattg atttcagatg ctgtgattat tcagtcgatc gaaaagatgg gcgattgatc
    19081 tcaggaagta agacacaacg acagtatttt tctcagatat ggtattttga tttttctgag
    19141 aaagaaaaaa gatggcaagc cgattttatt cagccgatta tgctggattg acaggaaata
    19201 tttatgaacg gagttgtgac aaaagccttg tcgcagctcc tttttctgaa taaacggtgt
    19261 gccagagata gcctatcggc aataaggcac gattttgtaa aatatgggaa gcgatttttc
    19321 aaaatcgctt cttatcgctc aaggctgaac atctctgtca caacctctcc actaaatggt
    19381 gtttaaaagc ttgcatctgc gataataaga acaaccaaac gaaaaatagg aagcactatt
    19441 ttagtttagt tgttcttatt acttgatgca ggacagcttt ttcacaacct cttttttgta
    19501 tagatgggca aaatttttgt aataataggc gtatgtcttt ttcgttgagg agatgaaaaa
    19561 gcatatggct tattttttgt ttgctttgct tactttcttt ccacttcaag tggcagaaat
    19621 atctactttt tattatgtaa gcggaaccat atacttgaaa tcagaaggag atcatttcta
    19681 tgaaaacaag aacaattgaa atacttcaca gaatcatgac agcccaatat ccagtaaaag
    19741 caaaacatct agcaagtgag ttcaatgtca gtcagcggac gatccgtcag gaagttcttg
    19801 aagcgaatat ctggttgagc agtaaaaagc ttccggagat ccgtacaatt cgcaacaaag
    19861 gttttttgtt gaagctggaa aaggaaaagc aagctcgatt agaaactgaa ataaaagatg
    19921 tgaaagacgg ctttttgaat cggcgcgaac gtatttttga tctagtttta gccgttgctt
    19981 acagccgaat cccgatccat ctaaacaaaa aagaagaaga atttcaaatc tctaaaagta
    20041 cattagatga agatatgaga cggttacgag caacactggt cagttatggg atagagatcg
    20101 tcagttatgg aaagcaagga ctggtttata aaggttcaga acgatctatc cgtacgatga
    20161 tctatgattt tatcaatcag aatctgggaa tgaccgactt ctttatgaat caagatgcac
    20221 aattaacacc ggctcaaaaa atctttctcc attattttcc tttggtggaa atcaaaaaaa
    20281 tacaggagat ctattccaat aagttgttga aacaagaaga tgatatttat aaaaaacaaa
    20341 tcctgctttt tacattgatc tggatccagc gaatcaggaa acatgaattt atctctgcca
    20401 ttaattggaa aaatacagaa ataaaagaca gtagcttttc tgcatttatt gaccacatga
    20461 tcgaggaatt tcaattggac aatgtcccgc aagtagaaag aaattatctt cgtttcacta
    20521 ttgagacgtt caatgctaga gatatctcca attcaatcga gtgggtacaa gctcaattac
    20581 taacgatcca attgatccaa tttgtagaga acgagacaca cgtacctttc catctaaagg
    20641 aagaagcttt attcgaaaat ctctataagc acatggctgc actgattgta agaatcaaga
    20701 atcatatcca agtcatgaat ccattgaaag aaaacatccg aacaaattat ttcccaatct
    20761 accgcgcagt cactcatttt gttcctacga tcgaagaagt aattggtggc aagatactgg
    20821 aggacgagat agcttttctt gtcatccact tttcgacgat cgccagtgta atcaagcgag
    20881 acttgaatta tgtttataaa tcagtcgttg tctgtaacca tgggatggct actggaaatc
    20941 tgttagcaga gaatctgaaa gaaaagtttc ctcagatcga gattactgca gttttaagtt
    21001 caaaagaaat cgaattagta gataaattgg atgtcaatct ggttttcagc acctttcaat
    21061 tgaattatcg aaataagccg cttttagttg tcgatccgat tttaactgag acgaatatct
    21121 tggttataga agactttttg aaaagaaatg aagaactaca gcggttagag cctaacagtc
    21181 atgaatcgac ggagttattc actcaaataa tagatgtcat gaaagaaagt ggtggccgag
    21241 tagatcgaat catctatggc aagttagaaa aattattcga aacaaacaat ttagaaatca
    21301 acaaaaggga gatccagcct atgttaaaag atattttgac ggacagcaat atcatgatca
    21361 aagaaaaagc cactacttgg gaggaatcga ttgaactagc tgctagacca ttggttaaag
    21421 aaaacattgt cgaagagcgc tatgtcgaag cgatgattcg tgcagtgaat gattacggac
    21481 cgtatatcgt gattggcaaa cacatggcac tagcccatgc ccgaccagaa gatggggtca
    21541 ataaattagg agtcagcgta gctacgattg agcaaccaat cgatttcggt aatcctgaaa
    21601 tggatccagt aaagattatt ttttgtttgg cagcagtaga ctcctactcg catctgaaca
    21661 tcatgaaaga actaattgaa ttgatcaaca acgaagagaa gttggatcga ttgattgaat
    21721 gcacaaatat cgaagatttc aaacaactgc ttttttcagt agttgagaaa taataaatga
    21781 tacgggggag aacaaagatg aacaaattga acattttatt cgtatgcggt gcaggtttag
    21841 gaagtagttt tgcagcacag atgtctgctg aggatgtttt gaacaaatta ggagtagaag
    21901 ccaaactgga ccattgtgat atttcttccg cagtttccat gaaaccagat gtgatcatca
    21961 cagcagaaaa ttttcgttcg cagttcgaaa agttcgcagt tgatcctaaa acggcgatga
    22021 tctatttgaa aaatattgtt tcaaaaaatg aaattgaaga aaaaatcacc ccagttctgc
    22081 aagataaggg agttatctaa atacaaaaag aaaagagggt aaaaacatgg gcgttatcaa
    22141 ttttatcatc gaaaatattt tgactcaggc atcgatcacc attgcattga ttgctatgtt
    22201 gggtctacta ctgcagaaga aatcagcagg acaagtcatc tctggcacgt taaagacact
    22261 cctaggattt caagtgttga gtgccggttc gagcatcatc gttggaagtt taacttattt
    22321 cgggaaaatc tttactgaag gatttcatat gcaagggatc atcccttcta ttgaatctat
    22381 caatggtcaa gcaatgaatg atttgggttt aggtagagat attgcactga cgttcttagc
    22441 catttttgta ttcaatatta ttttggctcg tttcacaaaa tggaagtaca tttttttaac
    22501 tggtcaagca attttatgga tggcaacgat gacgacagtc tttggctatt ttgccggtct
    22561 gcgagggatc gtattgatct tggtcggtgg ttttatcgga gcttgtttcg ccattgctat
    22621 gccggcagta gcgcaaccga tcattcgtaa aatcactgga tcaaatgata ttgctttagg
    22681 tcatttttgt acgatcgggt acttatttga agccggtgtt gctaagcttt ttggtgagaa
    22741 aggcgaaaat aaaaagtcga tcgaagatat caagttgccg acacatttcg aatttctgca
    22801 agatacttat ttgtctgtca tggtcgtgat ggtcccgcta tatatcatca ctgttttatt
    22861 tgcaggcgaa ccattcgctt ctgaattatc aggagatcaa aactatatca tgtttgcctt
    22921 tttacaagcc atccaattcg tagtgggtgt gtatgtattg ctagctggtg tacgtctact
    22981 gttaggtgaa atcgtgccgg catttcgagg gatagctatg aaattagttc ctgatgcgat
    23041 ccctgcctta gattgtccag ttttctttcc ttatagccca aatgctgtca ttttaggttt
    23101 cattacgaca acgattggta cgattattgc catgtttact ttgcccatgt tcggcttagc
    23161 gatgatttta cctggtatgc taaccaattt ctttgccgga gggacagcag ggatatttgg
    23221 caacgcagtt ggcggacgcc gaggagcgat cattggcggt attgctcatg gtttctttat
    23281 tacattattg cccgctttgc tagtaacgat ctttaattcg atgggattca acaatgctac
    23341 agcaaccgat gtagatacag tagccgcagc actcttatat gcctggatct taagcccagt
    23401 tttgaaagca ttttaattga aagtagggaa aatgatgttt ggatttttta agaaaaaaga
    23461 aaaagaaccg aaaaaagttc tggcaaaaga agaaaaagcc ctatcgccag aagcgaccga
    23521 aaagattcaa tcagagatta cacaattgaa acaagagatt tccactaccc aagataagca
    23581 taaattagcg aaactttatg aacaagttgg acttaaattt tctgaattgt atgtgaacga
    23641 tcaagccatc caatatttag aaaagagttt ggaaaataaa cagacgatcg gagatgggta
    23701 taaaaaactg atgagcctct ataaccagaa gcgtgcagat gctgctcgtg ctggtgatga
    23761 tcaagggatc gattactaca tgggcaaaat ggatgagatg cgacaaattg caaaacaagt
    23821 aacgatcaaa ggaaacaaat aaaaagataa ggtggcaata aaagatgtat acaacattga
    23881 aagaagtaac aaaaacagca gaagagttga attttacagt tggagctttt aatacgcata
    23941 acctagaaat gcttcctgct atgctaggcg cagcaaaaga aatgggttcg ccgatcatta
    24001 ttcaaacaag tgtcgatacc gctaaatata tcggcatgca tgtgttagtc aatgtaatga
    24061 cagctattgc agaagaagaa atggttgatg cagtcttgca cttagaccac gcccgagact
    24121 ttgatgatat caaagaagca atcgacagcg gatatacttc tgtcatgtat gatgggtccc
    24181 atctgccttt caaagaaaat atcctgaaga caaaagcagt agtagaatat gcccatgcgc
    24241 acggtgtttc cgtagaagga gaacttggaa caatcggcgg tacagaagaa ggaatccatg
    24301 tcgctgaaaa tgacaaggta tatacaagac cggaagacgc agtgaaattt gtagaagcta
    24361 caggcgtgga tgcattagct attgctatcg gtacgaatca tggacaattc aaatcaaaaa
    24421 cagaagtaaa cattcccttg ctgaaagaaa tcgatgcagt agttgatgtc cccctagtta
    24481 tccatggagg aacaggtgtg aaagaagaag attatcctga attgatcaat aacgggatcc
    24541 gcaaattcaa tgttggaacg gagcttttag tcaactggac gaaagtagct aaagaaacct
    24601 ttagtgaaac agaaatcaac aagtctcttc gccataatgt gattccagca aatgaagcag
    24661 taaaagaaat tgtcaagcat aagattggac tgtttatgaa cttggaaagt ccaattaact
    24721 taggaaataa atgagatgaa aaagtatttc attcttctgg ctggtagtcc tggtacagga
    24781 aaaacatatc ttatgaataa actacgggaa cgatttccag atatgtatgc catcacgctc
    24841 gatgaaatca aggaatacta tgcagaatcc atcgggttta acaatttgga agaacgggcg
    24901 gaacaagaaa ggacgaaagt ttatcctttc tattacaaag cacttgattt gtatatggaa
    24961 gcaggaaaga aagtgattgt gtccgagtat ccttttagtg ataagcaaaa agcacgactc
    25021 caagaattag cagatagata cggatatcag gtgatcacga ttcgcctaac agcagacttt
    25081 gaagtgttat ggcaacgtcg gtacaaacgt gatcgtgaat cagatcgtca tttgagctat
    25141 atcatggatc attatcacta tggtgacgta ttggaagatc gaacgaaagg gaccaatcat
    25201 attacgaaag aagcattcaa aaagatcatc gccgaccgaa aatatgacaa attccagtta
    25261 ggaacactat atgaatttga tgtaacagac tatactcaag tggattacac tccactattg
    25321 gataaattag aaaaagaaat caatgacaat aagtaacaaa gaaatggctt aatatcgaaa
    25381 aattggcttt tgaacgctgt ttaccgagag gtcgtgagcc tgtcgttcag ctccgagtca
    25441 caaggagcaa tttcaaaaaa acagcttctc atgtttgatg aatattgtga cttgtgaccg
    25501 aggagttggt aggagaacac cgtttagtgg ggaggttgtg aaaaagctgt cctgcctcaa
    25561 ttaataagaa cagccagaca agaatagctc gtcatatttt tcgtttggtg gggcttatta
    25621 ccgcagatgc aagcttttga acgccattta ttcacagccc cagagaagtc gaatgtcttt
    25681 tgttctcgag ttttttaaca ttcttttatt tattgcagat ttcattatat ttatttgccg
    25741 taattaaacc agtcttctat acttgattac atagtcatac atttgctaaa aaagaaggga
    25801 agagaatgaa caagaagttg gctcaataca aaagactact cgcactttct caagctggtc
    25861 tatattatgg aaaagatgta tttgataaag agcgttttga ggagatccgg acgatttcat
    25921 tggaactgat cagtgaaata ggaaaagaaa ctttcgaaga aataaaagcg ctcacaacga
    25981 ttggagaagg acatccaaca ccaaaagtag atgttcgtgc ctatatcaaa aaagatggca
    26041 aggtcctact aatcgaagat aaacggacga aagaatggtc acttcctggt ggatttgctg
    26101 aaataggatt gtctcctgaa gaaaatgtgc gtaaagaagt ctatgaagaa acaggattaa
    26161 cagtagaaac tactcagttg cgagcagttt ttgatacgaa taaacaaaaa gatattccac
    26221 agctttttca gtattataaa cttgtttttg cttgtgcgat ccacggtgaa gaggccaagt
    26281 ttattgagaa taatgaaact tccaacatgg gattttttag tatagaagag ttgccaaagc
    26341 tatcagaaaa gcgaacaacg aagaaacagt tacttatttt agaaaacaaa aaccagatct
    26401 attgtgattg agctttttcg taaaagaa
//
LOCUS       DANKHL010000013        25536 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000013 DANKHL010000000
VERSION     DANKHL010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.015"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="PW101_000392"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="PW101_000392"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL2512823.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="PW101_000393"
     CDS             complement(937..1518)
                     /locus_tag="PW101_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2512824.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="PW101_000394"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="PW101_000394"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDL2512825.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="PW101_000395"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="PW101_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDL2512826.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="PW101_000396"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="PW101_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDL2512827.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="PW101_000397"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="PW101_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL2512828.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="PW101_000398"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="PW101_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL2512829.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="PW101_000399"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="PW101_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDL2512830.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="PW101_000400"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="PW101_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDL2512831.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="PW101_000401"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="PW101_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDL2512832.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="PW101_000402"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="PW101_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDL2512833.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="PW101_000403"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="PW101_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDL2512834.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="PW101_000404"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="PW101_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDL2512835.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="PW101_000405"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="PW101_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDL2512836.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="PW101_000406"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="PW101_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDL2512837.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="PW101_000407"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="PW101_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDL2512838.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="PW101_000408"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="PW101_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDL2512839.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="PW101_000409"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="PW101_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDL2512840.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="PW101_000410"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="PW101_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDL2512841.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="PW101_000411"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="PW101_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDL2512842.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="PW101_000412"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="PW101_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDL2512843.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="PW101_000413"
     CDS             13972..14157
                     /locus_tag="PW101_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDL2512844.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="PW101_000414"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="PW101_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDL2512845.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="PW101_000415"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="PW101_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDL2512846.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="PW101_000416"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="PW101_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDL2512847.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="PW101_000417"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="PW101_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDL2512848.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="PW101_000418"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="PW101_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDL2512849.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="PW101_000419"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="PW101_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDL2512850.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="PW101_000420"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="PW101_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDL2512851.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="PW101_000421"
     CDS             18232..18879
                     /locus_tag="PW101_000421"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDL2512852.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="PW101_000422"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="PW101_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDL2512853.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="PW101_000423"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="PW101_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDL2512854.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="PW101_000424"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="PW101_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDL2512855.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="PW101_000425"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="PW101_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDL2512856.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="PW101_000426"
     CDS             20317..21255
                     /locus_tag="PW101_000426"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDL2512857.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="PW101_000427"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="PW101_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDL2512858.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="PW101_000428"
     CDS             21935..22774
                     /locus_tag="PW101_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2512859.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="PW101_000429"
     CDS             22750..23619
                     /locus_tag="PW101_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2512860.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="PW101_000430"
     CDS             23609..24406
                     /locus_tag="PW101_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL2512861.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="PW101_000431"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="PW101_000431"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL2512862.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         8299 a         4712 c         5497 g         7028 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggta gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttgaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcgagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttataa aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcatacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgattgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac cctcttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaag tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacatcctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tgtgacagaa gtggtcagct
    25381 tcaagaaata agaaggaatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag gagctacttc tggaacaccg tgaatcagag gttgtgaaaa agctgccctg
    25501 catcaagtaa taagaacaac caaacaaaaa tagctc
//
LOCUS       DANKHL010000014        24752 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000014 DANKHL010000000
VERSION     DANKHL010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.016"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(62..1093)
                     /gene="queA"
                     /locus_tag="PW101_000432"
     CDS             complement(62..1093)
                     /gene="queA"
                     /locus_tag="PW101_000432"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDL2512863.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1286..2461
                     /locus_tag="PW101_000433"
     CDS             1286..2461
                     /locus_tag="PW101_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2512864.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2465..3106
                     /locus_tag="PW101_000434"
     CDS             2465..3106
                     /locus_tag="PW101_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2512865.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3103..4020
                     /locus_tag="PW101_000435"
     CDS             3103..4020
                     /locus_tag="PW101_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2512866.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4017..4688
                     /locus_tag="PW101_000436"
     CDS             4017..4688
                     /locus_tag="PW101_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2512867.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5229..5717)
                     /locus_tag="PW101_000437"
     CDS             complement(5229..5717)
                     /locus_tag="PW101_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HDL2512868.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5918..6184)
                     /locus_tag="PW101_000438"
     CDS             complement(5918..6184)
                     /locus_tag="PW101_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL2512869.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6184..6447)
                     /locus_tag="PW101_000439"
     CDS             complement(6184..6447)
                     /locus_tag="PW101_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2512870.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            6923..7108
                     /locus_tag="PW101_000440"
     CDS             6923..7108
                     /locus_tag="PW101_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2512871.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7250..9073)
                     /locus_tag="PW101_000441"
     CDS             complement(7250..9073)
                     /locus_tag="PW101_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDL2512872.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9671..12376)
                     /locus_tag="PW101_000442"
     CDS             complement(9671..12376)
                     /locus_tag="PW101_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDL2512873.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(12682..13629)
                     /locus_tag="PW101_000443"
     CDS             complement(12682..13629)
                     /locus_tag="PW101_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDL2512874.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            13819..14088
                     /gene="rpsN"
                     /locus_tag="PW101_000444"
     CDS             13819..14088
                     /gene="rpsN"
                     /locus_tag="PW101_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL2512875.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(14264..14626)
                     /locus_tag="PW101_000445"
     CDS             complement(14264..14626)
                     /locus_tag="PW101_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2512876.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(14637..15758)
                     /locus_tag="PW101_000446"
     CDS             complement(14637..15758)
                     /locus_tag="PW101_000446"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDL2512877.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(15774..16124)
                     /locus_tag="PW101_000447"
     CDS             complement(15774..16124)
                     /locus_tag="PW101_000447"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDL2512878.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(16254..17765)
                     /locus_tag="PW101_000448"
     CDS             complement(16254..17765)
                     /locus_tag="PW101_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2512879.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(18087..19439)
                     /locus_tag="PW101_000449"
     CDS             complement(18087..19439)
                     /locus_tag="PW101_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDL2512880.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(19592..20668)
                     /locus_tag="PW101_000450"
     CDS             complement(19592..20668)
                     /locus_tag="PW101_000450"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDL2512881.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            20864..22609
                     /locus_tag="PW101_000451"
     CDS             20864..22609
                     /locus_tag="PW101_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDL2512882.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(22889..23326)
                     /locus_tag="PW101_000452"
     CDS             complement(22889..23326)
                     /locus_tag="PW101_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDL2512883.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(23310..24683)
                     /locus_tag="PW101_000453"
     CDS             complement(23310..24683)
                     /locus_tag="PW101_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512884.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
BASE COUNT         7055 a         5239 c         4635 g         7823 t
ORIGIN      
        1 ctttgtcata aaatctttgt aaagcttttt tgttattcaa aactagcggc ttttcataac
       61 cttatttcac gaacatcgca tctccaaaac taaagaaacg atagcgctct tcaatcgcat
      121 gctggtaagc agataaagtc aattctcttc ctgcaaaggc actaactaac atgactaaag
      181 tcgattttgg caaatggaaa ttagttgaga aagcttctac cactttgaat tcatagccag
      241 gcgtaatgaa aatatcagtc catccgctgt ctgctttgat ttctccatta aatttcgttc
      301 caatcgtttc taatgttcga attgaagttg ttccgacagc cacgattttc ccgccatttt
      361 tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa acggtagaat tcactatgca
      421 tatggtgttc ttcaatttta tctacgctga caggtcggaa tgtacctaac cctacatgta
      481 gtgtcagata gactaattca acaccctttg cttcgatctc atcaagtaat tctttcgtaa
      541 aatgcaatcc agctgttggc gctgcagcag aaccattttc tttcgcatag acagtttgat
      601 agcgatctgg gtcttccaga cgttctttga tatatggagg caatggcatt tctcccaatg
      661 attcaagaat ctctagaaaa attccttcat actcaaagtc aatgatccgt ccgccgtgtt
      721 ccaattcttc tttcactact gcttgcaatc tgccatcacc aaaagagatt cttgtcccaa
      781 ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt atctccttgt gtattcgtta
      841 gaagaaggac ttctaaatgc gcaccagtat ctggtttttc tccatataaa cgagcaggca
      901 gaactcgtgt attattcata actagcgcat caccaggctg taactcttca atgatttcat
      961 ggaaatgttt atcttccatc tctccagttt tccgatccaa aacaagtaaa cgagaatgat
     1021 cgcggtcttt taatggtgtt tgtgcgatta attcttccgg caagtcaaaa tcaaaatctt
     1081 ctgttgttaa catatcgtca ctcactttct atcatgtgct tcatcattct atcatgtttt
     1141 tcgttctgtt ggcaaaatca gcactttttc acttctattt tttgttttcc taaataaaaa
     1201 aactacccta tttgtatttt ttcattgttt taggcatact agtaatagat agttcttcat
     1261 tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa aaatttataa
     1321 aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg agttcatttg
     1381 ttttatcggt acgagcggaa gcggaaaaac gacgacgatg cggatgatca accgaatgac
     1441 tgatccaaca caagggaaaa ttttgattga tggaaaatcc atccaagaaa ttgatccagt
     1501 ggaattgcga cgtcagatcg gctatgttat ccaaagtatt ggattgatgc ctcacatgac
     1561 gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag aagaacggaa
     1621 taaaacagct gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt tagatcgcta
     1681 cccacatgaa ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg cccttgcagc
     1741 gaatcaagat atcatcttga tggacgagcc attcggtgcg cttgatccaa tcactcgtga
     1801 ttccttgcaa gaccttgtca aagatttaca agagcgtttg ggaaaaacga tcgtatttgt
     1861 aacccatgac atggatgaag cgattaaact agcaagccgt atcgccatca tgagtgaagg
     1921 ccgtgtgatc caatttgata caccagaaaa tattttacgt catcctgcaa acgatttcgt
     1981 agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga caagagtcgg
     2041 cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac aagaagcaat
     2101 caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt cgaatgtatt
     2161 aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat ccagtgtcgg
     2221 cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc gtgatacctt
     2281 gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac agaacaaagt
     2341 cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga tttggggaga
     2401 agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta aggaggtctg
     2461 acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag gttttgaaca
     2521 tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac cgcttggtgt
     2581 cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca gtgccttgca
     2641 gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg taggtaaatt
     2701 acctgcgatt atcgcactct ttatttattc attattgccg attttacgta atacgtacat
     2761 tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg ggatgactga
     2821 gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca tggctggtat
     2881 tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata tcggcgctgg
     2941 tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc tcattttggc
     3001 aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag gtctcttgga
     3061 acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg tatactaaaa
     3121 atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc tggtcttgcc
     3181 agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga agcacagatc
     3241 ttagcaagtc tagttgccgg aatggttgaa cattatacag ataaaaatac cgcaatcatc
     3301 aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga tgcttcaatt
     3361 tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc tcctgaaaaa
     3421 gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata cggtcaaacg
     3481 tggttcccgt cctatggttt tgaaaatacg tatgtctttc ttgttcgaaa agacaccgca
     3541 caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga attagtagct
     3601 ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt ccaagaaact
     3661 tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta tgacgcagtc
     3721 gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat cgccagttat
     3781 gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc tgctccagtt
     3841 gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa taagttaggt
     3901 cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa taatctagtg
     3961 gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga agggaagtga
     4021 agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag gaaaatggct
     4081 cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt gtcctctttg
     4141 cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa ttagcaaact
     4201 gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg atctccattt
     4261 taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt ttgtattccc
     4321 tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa aacatccttg
     4381 atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta gagttaccac
     4441 tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc atcgggataa
     4501 ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc cgaggaacca
     4561 atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca ttaatggcga
     4621 tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc agccgtacat
     4681 cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt cgcaaaggct
     4741 tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc cttatgggca
     4801 aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat actgccaacg
     4861 ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca ttttgccaat
     4921 ccattttccc atcactcgtc atggaattca taccacatga tctctaaata ctcgtctgct
     4981 tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat ttaagccttt
     5041 tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg tgactgccgt
     5101 atcggtaact tcgatcatct tttatacaat caggaacttg ccatcctcat ccttggctaa
     5161 aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct aaaaccggta
     5221 cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg atccttcacc
     5281 agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa gacattggcg
     5341 taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat ggccgaaagc
     5401 gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt cgctttggat
     5461 acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc ttttccaaaa
     5521 gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt gtccattgct
     5581 tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt cttcgcctca
     5641 ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc tccaatttta
     5701 ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca acattccatt
     5761 cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta aagaaatcat
     5821 gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt tggggagact
     5881 ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt gtccaggcag
     5941 aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga tgctgtctat
     6001 tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa gactgcgtgg
     6061 gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc tttaaattcg
     6121 tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact gtatagttac
     6181 tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt cgtctttttc
     6241 gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt cttgaatcgc
     6301 tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac taataatctc
     6361 gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt tacgagcagt
     6421 gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata taattatagc
     6481 gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc aaacttttcc
     6541 tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca cgctcacttc
     6601 ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac gtatcatctt
     6661 tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac acgaaggttc
     6721 ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca cgaaagattt
     6781 gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca aaaatgattg
     6841 cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt agaccgttgg
     6901 gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat ggtcacttga
     6961 cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg taaaggaaga
     7021 agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt atgatcaaga
     7081 catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc atacgctgtt
     7141 cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa acagaccctc
     7201 tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt tatttcttca
     7261 aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc aatcttaacc
     7321 gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca aattgaggaa
     7381 tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata tctacataac
     7441 ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa cggacatctg
     7501 ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct tcgtctagcg
     7561 aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac gcaccgatat
     7621 ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa tcatgtagtt
     7681 ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca tggatcttat
     7741 aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc cctaaccgat
     7801 agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata ttggcaatcg
     7861 atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct gtttgagata
     7921 aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct gttgagccat
     7981 ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca ttggaatagc
     8041 ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt gccagattgt
     8101 atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa atcagcgctg
     8161 tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta ccaatttgag
     8221 acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata aatgtgatcc
     8281 ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg gcattttttg
     8341 ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca gttaaagagg
     8401 aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc acagccgctc
     8461 ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat aaaccaacaa
     8521 cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg aacgatgcag
     8581 attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc acagaaatag
     8641 cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca cctgctgaga
     8701 cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa atcaagccag
     8761 cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga ggataagcat
     8821 ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca aaagcggcaa
     8881 tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca acgatttgtt
     8941 ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa gcagcaaatc
     9001 gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact aacaaccttt
     9061 ttaagtaatc caatttctaa actcctttac acacaatttt acttcaagtt ttatagtcga
     9121 aaatttatag taaacaagat gttatagtac gctcactgac cttaaaagtc aataaacttt
     9181 aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg ctttaataca
     9241 gcaaaaatct aacatttttt tatgggcttt ttctttgaat tctataataa aaggatagaa
     9301 cttgacttag aaaatcaagt tctatccttt ttgatatctt aacttttgga agagagatgt
     9361 aaggctgctt tctacccatc attcttacta ctcgaagcat agcgcagttg tcacaacctc
     9421 ttgataaacg gtgtttaaaa gcttgcatct gcggtaataa gcccaaccaa attaaaaaca
     9481 taagaagctg tttttatttg gtcgttctta ttacttgatg cagagcagct tttttacaac
     9541 ctcttggtac acggtgttca atcagctgac cctcggtatt agctcaaaaa aacggcaaaa
     9601 tatgaggaac tatttttcca tttttttctc taatactcag gtcaaaacac tgatttcaca
     9661 acctcttttt ttaatgaaaa atatggctct catgcgccgc ttctttgaat ttcgtcattt
     9721 tttcttcgat gaacggatag attgccgttc ctacgattcc aaagacaaca aacaaaccga
     9781 tcatgatcca gattgctttc gtagcatttg gccaataaat tcctcctgca gattcccgta
     9841 acaagtttac cgcatgagta aatggcaaga acggattgat catttggaag aatttccctg
     9901 agacttggat cggatagttc ccgcctccgc cagagatcga cagtactaag atgataatag
     9961 cgatcccttt accgacatta tcgaataatg cagccaagac atagacgatc atcatgaacg
    10021 ctaaggcgat caagacagca aatgacacgc tgtagaccgg attttggaca tctactccta
    10081 gtaagaaaat attccctaat gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg
    10141 tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta
    10201 aatagtattc tgtcgtagca acacttgaaa gaagaacagc gccgacccac agacatagtg
    10261 cagtataaaa cggggtgcta gctgaaccgt tatttgcgat tggataaagc gcattggttt
    10321 gtagctcaac aggttgtgta aagaagtcac tttcttttgt tgcgtctagt ttcaataatt
    10381 tgatgacttc gcctagatcg acttcttgtt cgccttttcg gatcgcgtct gctgcttttt
    10441 ggataccagc tttgatattt ggccagtcat tgttgatcaa gtccgcagca gcgttcacag
    10501 ctgtttcgac ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg atttcatttt
    10561 ttagtgctgg ccaatcattt tggataaaat cagcggccaa tcctaatttt tgctgaatga
    10621 tcggtaattc attgttgtat aggtctgccc cttcattgat gccattgacg atggtatcca
    10681 tatttccatt cagcattgta ttggcatcat ggatttcttg tccaattgca ggcatttctt
    10741 tttgatattt ttctaaaatt gccactgcat tcgttacagt tgctttcgtt gagctgagaa
    10801 gcgaatcaaa gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat
    10861 cgatgacttg gtctagtaga gaagtgacag tggaactgat ttgacctgta ttgataccag
    10921 caatgatgcc attgatatta ttaacaacgg catcaatttg atctagcaat tcatcaatag
    10981 ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga taggcggttt tgcagattgc
    11041 ttagcaaggg ttttaattga tttagctgat caataattgg ctgtaatttc gtatttccag
    11101 cacttgtttg caatttagtc aggaactcga ccagtttatc gattgcattt tgttgttgag
    11161 atattccgtc aataaagccg ttcaatacat tgctgatgtt tttcttttca tcatcagtca
    11221 gcttaccatc ttcaatagct gttcttacag tatctattaa cgaacctgtc gttgttgcca
    11281 cttgtccaat tgacttcaat gtaacatcta cactgtttgt gatgcttggt atcgcatctt
    11341 tcagctgaga ggcacctgat tttgtttggt ttgctagatc acttgcctga ttttctaaat
    11401 ttttcacatc tggcaagata tcttgtactt gttggatgat cgtcaagcct tgttttgctt
    11461 catcgattcc ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta
    11521 gttgcttacc ggcattttgg atctctggga ttttttcttg caaatcaagg atgatttttg
    11581 cttgatcttt caactcaggc attttgtcat tcaatgcaac gactttttca cccatctcat
    11641 ctactttagg gatgtaatcc acgaattcat ttgctttatt cagtttttcc ttgatttcag
    11701 gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt
    11761 catctgttga taaaatcata tctttaactt tattgatact tactaggttc gtatcgatgt
    11821 cgtaacctat ctcattgaat actttcaata aagtcgaact ggctgttttg ataaattcat
    11881 ttgtgatttg cgattggatc gaacttgctc ctttatcggt gattttcggt gcaatcgcat
    11941 tgattttttc atttaccgta tattcgattt ttggtttctt gatatcacca ctagtaaaac
    12001 tcagcagatc ttctgagaaa tcctttggca agtaaatacc tgcgtaatac tttccggaac
    12061 ggactccgtc ttctaattgt tcttttgaat caacgaattg ccatcctaat tgtttatttt
    12121 tatgaagcga ttcgatgact tgctccccaa tggccacttc tttaccttga aactctgctg
    12181 gcttgtctgc actgtaaaca gcaattggca gctctccagt atttccatat ggatcccata
    12241 atgctttgat gttaaaccac gcatatagcg aagggatgat catgatcgcg atgatcagaa
    12301 gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag caaatataat cgaagcgtat
    12361 ttttgattga ctgcatagtt ctcccccttc ttcttttttc agctgaatta ctttaccatg
    12421 tatttcatac ttttttcaat cggctttttt caaaaaagtc gaatttttgg acatatcacg
    12481 gcaattgtct cattgtaatc tcttacatat tgagaaataa ttaaggatta cagcctttac
    12541 tttatcatga aataaaaggg aaagataatt ttatgccaaa gtttttatct attatacgta
    12601 tacgcaaaaa agctaaaaca ttttttgtct ttgcccctac aaaaatgttt cagctttttt
    12661 cgttttttat tcctgtattt cttatttttc gaatgacagg atgattttcc cagcaggagg
    12721 atgtccttca actagctgat gtgcttggat gacatgctta agtgaagcag acagattcat
    12781 tgaaataaaa acatgaaagg ctcctttttg gtaggctttg attatgccag caaaggcttc
    12841 tgcatctaga tattcctttc ttggaacaaa cgattcataa aagccttcct ttttttgccg
    12901 caaatctaga tctgggaggt catttaatgc aacatacctg ccgcctggtt tcatgatctg
    12961 gatgccggtt tctcctttga tacttccttt cgtcgcatcg attacaaggt ctgcctgatc
    13021 agcaaattgc agacctggat ttgttttatc ataagcagca aatgctgaag ctcctaattt
    13081 ttttaccttt tcctcatttt tactggatgc actggttaag attcggattc ccttttcatg
    13141 gagaagctgg atcagacttg acccgaccgc acctgatgct cctagtatca ttacagtatc
    13201 tgtcggctgg atttccgtca aatggttgat tagattataa gccgtaattc ctggcgtgac
    13261 cattccggct gcttcttccc aactcatttt atctggaatt tttgctactt ttgctgatgg
    13321 caacacgact tcttctccgt aagtcccgcc gactgcatgg acagctactc gatcacctac
    13381 atgaacagtt gtgacatctg aaccaatctc tgtcacaata ccagcgccat cattacctgg
    13441 tacataaggg aatttcaatg ttcgtatctc tttcatttct cctaaacgca aggcaacatc
    13501 ataaggattc acactgaatg cttttatttc tacacgaaca tgtccagaac cgacttctct
    13561 gggatgagca tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa
    13621 tcttcgcatg gtcaccatct ccctttttct ttaagtttac gtttatgcct ttattttgac
    13681 aaaaaaaatg tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt cttttttcgc
    13741 gtaacgtgtt aaattattta tctaaaagcg taaagattac gatttacgct aataaaaaat
    13801 aattggagga atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa
    13861 gaaacgattg aacgctatgc tgctttacgc caggaattaa aagcgcaaaa agattacgaa
    13921 ggcttgcgta aacttccttt agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc
    13981 gatggacgcc ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa
    14041 ttagcacata aaggtttgat tccaggagta aaaaaagcca gctggtaatc caagtctggc
    14101 tgtaatgtaa aggaggaggg cgcatggacc actccttgga tcatcaaaaa agatacgttc
    14161 gcttcttcta gcagaaatgc tggtactaga agcagacgta tcttttttta cgcacagctg
    14221 gtcctttttt atatgtaaag gctgcatggt tacttcgcta tatttattta gtaacaggag
    14281 tcagtgcttc gatacccaca ctaattttca atgctcggtc aacttctgcc ataatgggtc
    14341 gatccaaatg gcagaccttt tctttcaatc ttgatttatc gatcgttctg atctgctcca
    14401 acaggataac ggagtctctt tctattcctg ttccagtagc tttgatccca atgtgagtcg
    14461 gaagcttagg tttagccatt tttgccgtta tggccgcaac aatgatcgtc gggctaaaat
    14521 ggttacctag attattttga ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca
    14581 cgactggaga aagatcagca aaataaatat ctcctctttt taccatagga tcctccttat
    14641 tcttgatatt ctcttggtat ccgctgtcct aatccgcaag ccacttcata gtggatcgta
    14701 cctaattgat ctgctatatc ctgcatagtg atttctttgt ctgcattttt gccgataagc
    14761 gtcacttttg ttccgacagg aaattcttga ggcaatcgga tcatcagttg atccatacat
    14821 acacgcccaa tcgtttcaca gtagttccct tctaccaata gagaaaaacc ctgcatcttt
    14881 cgtggccagc catctgcata accgatcggt atcgttccga tccactctgc ttcaggagtg
    14941 atatatgttt ctccatagcc gattccttca cctgctggga gttttttgac ttgaatcaat
    15001 tcagaaacca gctcaagtgc tggctgcaat ggataaactt ccggtagtgc gtgcccagag
    15061 ggattaagtc cgtacattgc tactccataa cgaatcatat tgccgattgt ttcatcatgc
    15121 cataaagccg ttgcactatt gctgacatgt acatatcgag gaagttcggg aagttttttc
    15181 agcacttcta taaagcgttc tttttgcaga ttccaatatg tatcatctgc ctgatctgct
    15241 gtagaaaaat gggtaaataa accttcccat tctaatacac gactttcttt gatgaatgca
    15301 gctgcctctt ttacttcttc aggtgtacaa aatccgatac gacccatccc agtgtctgct
    15361 tttagatgaa tcgaaagagg cgtctctagc ttacttttct ctaaaacagc agctgctgct
    15421 tgcaaccatt cccgtgtacc tgcagttact gataaatcat gagcaacagc tacagtcagt
    15481 gcgtcgtaag gaaccatatt caagataagg atgggctgag taaatccagc ttctctcaac
    15541 tcgactcctt catctaaatt tgaaacacaa aagcccgaag ctccacctgc aactgctgcc
    15601 tcagctgttt caatagctcc atgaccgtat ccgttcgctt ttactactgc aaacaattct
    15661 tttccttgcg gaagtcttgc aacttcattt gctacatttt cagttatagc ttttttatgt
    15721 atcactgctt tcgttgggcg gtgccaagcg acaaccatcg tttttccttc tttctagtct
    15781 tccaaaataa tttgtgcaaa agctgtttca tttgtatgag taatactgac ccatacattg
    15841 ccttcatgag gtgaacgcgt cacaacaggc tgaccatttt cattttttaa aatctcgaca
    15901 tcttgaaagg taacttttcc gatcccagtt ccccaagctt tagagaaagc ctctttgcaa
    15961 gcgtaacgcc cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg gaatagtttc
    16021 atttcatcag aggtcaagat tcgtgcaatg aattttggtt tttcttcgat cagccgagtg
    16081 atcctaggaa gttcgactgc atcgatccca attcctttta tcatatgctt ttctcttttc
    16141 aataaattta gaacactctc attctaccaa attataacca ttttgccaca aataagtcca
    16201 caaataacaa aaatgaaagt ttctcttaaa aaagagaaac tttcatttta tgtttaatcg
    16261 ttatttgaac ggattacaaa tccgcgtttt ttatcattgt gacgtttacc gccatctttt
    16321 tggctgttat tatttttgct ataaccgcct tttgatttgt ttccacgata attaccgccg
    16381 tcacgacgat tacggttgtt tccgccacca cgtttgccat ttttattgta tccttttttc
    16441 tgcattggca atgggcgttc tggtgtgatc ttcactggca ctgcatcagc tggatctttt
    16501 gctgtcgttt ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc taataattta
    16561 tctgctgttt gcaggtattt atctagacca ttttctgcaa gttttgtttc gatttgttca
    16621 actgcagcac ctaattgacc tttgaatgct tctttttcgg ttggtggacg taaagtcgtc
    16681 atccgttttt tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg
    16741 aaagtcacag acataccgcc ttttccggca cggcctgtac gtccgatacg gtgaacgtag
    16801 ctttctggat cttgagggat atcatagttg tacacatgag tgacacctga aatatctaat
    16861 ccacgtgcag caacgtctgt cgctacaagg atatctaaat gaccgctctt aaatgaacgc
    16921 aatacgctca ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc acggtaaccg
    16981 cgtgcttcta atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc gaagacgatt
    17041 gttaattcag gtgtttgaac atcaaacaag cgagtcatga tatcgaattt ttcgtattct
    17101 tttgcgcgga catagtattg atcgatcaag tcagctgtca tttctttcgc tttgatcttc
    17161 acatgatgtg gatttttcat gaatttaacg ccaatattct tgatagctgg cggcatcgtt
    17221 gctgagaaaa gtaatgtttg tctttgatca ggaacttgtg aaatgatttt ttcgatatct
    17281 tccaagaagc ccatattcaa catttcatct gcttcatcca aaactaatgt ttgaacggtt
    17341 ccaagtttca atgtgtggcg gttaatgtgg tcaagcatac gtcctggtgt acccacaacg
    17401 atatgtggac gatctttcaa gccacggatc tgacggccaa tatctgctcc gccgtaaacg
    17461 gcttgtacac gtactttttt gtcgcgccct aaacgatata attcttcttg tgtttggata
    17521 gctaattcac gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc tggatcgatt
    17581 ttttctaaca ttggtaatcc aaatgcagct gttttccctg tacctgtctg tgcttgtcca
    17641 ataacgtctt ttccagctaa tgctagtgga atcgtttctg cttggatagg cgttgcttct
    17701 tcaaaaccag cgcgttcgac tgatttcaac aattctggtg ataattctag ttctttaaat
    17761 ttcaaatgaa tcctcctata tggttgttaa ctgaggatgt agcttttttg ctaccccgtc
    17821 gatttgttcg gtcaaagtgt attgtttctt tttgacagtg gccacgcacg tttttatcca
    17881 ttcttagccc ctgtggcacc accgaaaaat cactttgcga agtttaggca ttagaaaagc
    17941 caaacgggtc gcgttcagct ttatcattat tatgacatct ggcttcaaca agttgtttct
    18001 atggcaaaac cagtccaatt tctcttcatc tttcggcaat aggttattct aacatagttt
    18061 ttcgttttct gcaagtttac agatttttat ttcatttgct gaagttgttc taccacttca
    18121 aataagccca tgctgttgct gccttttagc ataatactat ctgttgcttc aagattttgt
    18181 ttgatagcat caatcaattt attcttttct ttttccaaat agaacacagg tagttggggg
    18241 tatttttggt tcaattcttc ttttagtgca tgcatttgtt ccccataaag gaaaatcatg
    18301 gcgaatttct caggatcgat atgttcactc atttgcgcat gcatatttaa agactctttc
    18361 cctaattcca gcatatctgc taacacagcg attctgcgtc cttttgttgg cagattttta
    18421 aaagtatcta ataccagtcc catggctgtt ggatttgcat tgtagacatc gctcaaaata
    18481 gctgcaccat ttcctgccgt tagccattcg gttctgtttt gagtgataga aacatgggcg
    18541 agcccttggc gtatctcagg tatggacagt ccaaagaagc gcccgatcgt atacgcaatc
    18601 aaagcattcg ttacattata acttccaggc agcgggatcg taaatgtttc tccttctact
    18661 acaaaatcgg tttgtgcttt tccttttgct gtcacttccg catgaacatc gccatttcca
    18721 ataccgaatg ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag caacggttca
    18781 tctgcgggca caacgagtaa tccgtctgat acaagtccgt cagtgatttc cattttggct
    18841 ttagcgattc cttctcgcga cccaagattt tctatgtgtg cttcaccgat cattgtgatt
    18901 gctgcagctt ttggttgagc cataagtgaa agttccgtga tttcattggc gtgatccatg
    18961 cccatttcca aaacgatctt ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga
    19021 ccaatattat tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc agacaagaca
    19081 gacgcggtta gatctttcgt ggtggtttta ccattgcttc ctgtaattgc aacgacttct
    19141 gcgcctactt tttccaagta ataggttgcc aattgctgca tggctttcaa aggatctgct
    19201 acaggaataa ctggaaaatt ttttggagct tgttcaacag gcaagctcca aaatgaagca
    19261 gatgctccgt ttgtgatcgc ttgctggata aattcatgtc catctcgcgt accagcaaga
    19321 ggaacgaata aacttccttc ggttatcttc cggctgtcaa attcgacatt ggtaatcttt
    19381 gtcagtgcag agcattcgct gcccacggct tgtgcaattt cttgtattgt tagattcatt
    19441 gagatctctc ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg
    19501 actctcactt ccaagtaaat ttctctattc gtatttagaa gctagaatga acgcggttac
    19561 gcaatcactc cagcctcttc tttatttctc tttattcatt tttttcaaaa aaagattgac
    19621 gctgatggta tcgattcatt cctaactgga tcagttcttc aatcaaatca ccgtatttca
    19681 agcccatatt ttcccataag agtgggtaca tactgaactc cgtaaatcct ggcatagagt
    19741 ttaattcatt caggaataat tcatttttat ttgtcaaaaa gaaatcgcac cggctcaatc
    19801 cgcttccacc taacatcgtg taagctaact tcgcgtactc ttgcgctttt tgataaactt
    19861 cttctggcac ttcggctgga atctgcattt cgattttatt attgatatat tttgcttcat
    19921 aatcatagaa tgctacgtct tttacgactt caccaggcaa agtcgtccga acgtcttcat
    19981 ttcctaatac agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg attgctcgag
    20041 aatcatactg ataggctgtt gctaaagcat tttgcagctc ttctcggttt tctgcttttg
    20101 taatgccgac actagaaccc atattcgccg gtttgacaaa catcggataa agcaaagaac
    20161 cttcacattg atcaaatact tttttaggat tttctttcca ttgattctta agtactggta
    20221 cataaggaac ttgcggcaca ccagcagctt gtaaaatata cttggtcatg attttatcca
    20281 tggcacatgc actggtcaat acgcctgcgc cgacataagg catattcaat gtctctaaga
    20341 agccttggat cgttccatct tccccgtttg gcccatgtaa aactggaaaa acgatggctc
    20401 cttcttcttt gatttcgccc ggattgatca cttttcctgt aaagccttcc tctgtctgtc
    20461 cacttgggtc ccatgaaaga tgcaagacat ctttgctggc aggtttttct gttagtaatg
    20521 gacctttgac ccattgtcct tcttttgtaa taaatacgag ttgaacttgg taataattat
    20581 aataaatggc atttaaaact gaaaatgcgg aaagaatgga cacttcatgc tctgcgctgc
    20641 gtccgccata tagtaaagta atcttcaaaa gattgtcctc ctgtattcta aatcgttcag
    20701 tgatttactc tcatatttta gcacaaaaca aaaataaacg aataacttat ttataaaaaa
    20761 acgagccagc ttctaaaaga acgagcgtca tttatttttt gtcagctaga ttatttgtcc
    20821 cgttttttat aaaaagttat gataaagaaa aggagggatg atgatggcgc aaacaaatcc
    20881 acgcagccgc ttggcagaaa tcattcgagt gttcatcaat tacaatgtag tgccaaactt
    20941 tgtacagcaa aaaaatcccg aacaggtaaa aaaagccttt gaagaactag gccccacctt
    21001 catcaagata ggacagatgc tttctgttcg agaagatttg ctttcttctg ccttcactca
    21061 aacattcaaa acattacaag atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac
    21121 gatcgaaaca gaactttctc tttcattatc agatatattc gatgattttt caaaatcgcc
    21181 ttttgcctct gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg gtgacgcggt
    21241 agtcgttaag atccaacacc ctaacatcgc cgaagagatc cgacttgatc ttcaactatt
    21301 tgaacgagcg atccctttga tcaaatacat tcctgaaact agcgtagttg atctaaaagg
    21361 ggtactccaa gaagtaaaac gttccttgat caatgaaatg gacttcttaa aagaaagtca
    21421 aaatggcgaa caattctatc aaaagaacaa tggctggaaa gagatccgca gccccaagat
    21481 ttatgacgct ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt
    21541 gaatcattta atgaacgccg aaaacaaaac tgaaactttt atcactggaa ttcaaaacaa
    21601 gcagctgaaa caagaagttg caaagctttt agttgagaac tttatgaaac aggtgtttga
    21661 tgatggtttt ttccatgctg atcctcatcc agggaatctg ctattccatg tactaacaaa
    21721 agaagaacag acacaagcta gccgaaaaac tgaaacagtc catgaaaaag agttcggttc
    21781 ctttgctttt cgagcctcca cttctgcaga agaccctgtt gcgccatata ccatcaacta
    21841 catcgatttt ggtatgatgg ggcatctttc tgcaggactt aggcaaaaac tcacacaagc
    21901 cgttttagcg ctgtatacga aagatgctta tcgaatcgaa aaagcggtcc ttcgactttg
    21961 tcagcaagaa ggctcgtttg atgaaagtcg gttccaccaa gaactgacaa gttttcttga
    22021 acagtattat gattctccta ttgatgaaat caacttgcaa gaagtattca cacatgtcgt
    22081 gacgatttgt catcaaaata atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc
    22141 atttgggaca ttagaagggg tgatccgcgt cctcgatcct gaagtttccc tcatggaagt
    22201 cgcttcgcct tttgctcaac attattttct cactcatctg gatgtagaag atacattgaa
    22261 acaaagcgga ttagacttat tggaagggat gaaggctgcg cctaagatcc ctcaacagct
    22321 ccaccatcta ttagaaatgt ggacttcggg acaaggaaaa gtcaatttgg aattgaaaaa
    22381 gcaagacaaa ttattgagca ggatcgaaag tatgatcaat cggcttgttt tcggtatgat
    22441 tttagctgca ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc
    22501 agaggtcgtt tccttattag ggatatttac gtatgctatt gccgcattcg tcattatttt
    22561 tttggcaatc gatgcattga tccaaatgta taagaagagg aaaaagtaga aaaaggttgt
    22621 gacagaaaga atcagcttca agaaataaga aggggtttta cataaattgt ttctcaaatt
    22681 tttgtgaaaa ttcggcttat ttttcgaaga ggctacttct gaaacaccga gaataaaaaa
    22741 tgtgaaaaag ctgttctgca cccagtaata agaacaatca aaaagggaac atggaacaca
    22801 tttatcctgt ttccctgttc cctttttgat gtattcgagc ataaataaaa taccttcgtt
    22861 acctactatc ttctgtactt ggtaaacctt agtcaattgc cttttttaac gcttgacgaa
    22921 aagacatatg atctttctcc gtaaagggtt ttggaccctt tgtccttatg ccagcttttc
    22981 ttaactttcc actatgataa cgttgttcta gaagcgtgcc gatattcgct tcgtcaaatt
    23041 cttttccaga gtgatggaac actcgcacac ccttcggcaa tacaagtgaa gctaaaccat
    23101 aatcttgcgt gatcaaaaag tcattcttct taaccatacc gacgatttta taatccgcac
    23161 tatcagctcc gcggtctacg tagacgattt ggacatgatt agcgtattct ttggtcgtat
    23221 aatggtccac actggtcacg ataaccacgt caagaccata ggattctcct aattttatga
    23281 catcttcttt tactggagaa ccatctccat caatgaatag cctcattctt tttcgccttc
    23341 ttccataaat gataaacagt tactaatccg ctcagtaaaa gaccaacatt gatccatttt
    23401 gccaagataa gcagttgtac actttctatc ggcactgatt catggttcag cgtgatcaat
    23461 aatagatgga ttggctctcc catttctttt gctaaaacaa acaaaagcgt catcaataaa
    23521 aactggtgga tcacactccc tttattttga gtagtatact tgacaacgat ccggcgatcc
    23581 attggaatag ctgtatcagt ttgataatat tccttattta gccagctgcc tgttactacg
    23641 tgccagaaac taagcagaaa gacacctatc gcaaaaccat ttggaatcaa gaggaccaat
    23701 aaagaagaac tcagtccaag caattgcata gccaatggag accatgttaa acgtttcaaa
    23761 atccatggtc cgactatttt tgctgcaatc aagcctgctg cataaggcaa atacataaaa
    23821 atcgaaagtt tttctaatcc gtacagtatg ccaacataaa aactgctaaa taaaaaaagg
    23881 aaattcccta gcattccatt tagaaaagta aacagattta gccagcttgg caacttccat
    23941 tccttcttct ggtgtcctaa aacaaacacg aacaagatga acagcagcaa gaaaccataa
    24001 atagcaagat caaaaagcct ttcatcgaat aacaatcggg catttctcaa aagaaacaat
    24061 aagccaaaga atatcaaaaa taaaacaagc tctcgcgtag ctaccagatt tcttttgatg
    24121 tctttgaaat ccagttcgta acgcggataa tgactaaccg tatggtaggc catgacgtaa
    24181 aaaagtgtat aaatagtaaa gcttgctgca atctgagtcg gtgcaggcaa ctcaatcgct
    24241 tgatacagga gaaatagcca gatagctgca aataagtact ttttcccatc catattgata
    24301 aaaccttgcg tcttttcgtg aaaattgacc gtggtattag caggtacaag ccatgctgca
    24361 ctcagcccta gcaaaacact tgaaaaataa aataaaggaa aataaaatac gcctaatatt
    24421 ccaagcaatg aaccagcacc accaatcagc agagcgatca tcagcaacgt gtaactatct
    24481 agaccaaatc gtagactgtt gactaaaaat agtccagtca tccgaaaggc ataaaacagc
    24541 gtaaatggca aaatcgtcag ccaattatgc gagtctgtta gtcccaaaaa taaaagataa
    24601 ggaataaaaa taaccgtgtt acttaagata aagggcgttg tttgtgtaaa attacgccca
    24661 aatcgtttga gtttttcgaa catgacaatg gctccttaaa cagtcttata actaatctgc
    24721 tcttcgattt tagcattaaa ggggaaataa ac
//
LOCUS       DANKHL010000015        24690 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000015 DANKHL010000000
VERSION     DANKHL010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.017"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            97..825
                     /locus_tag="PW101_000454"
     CDS             97..825
                     /locus_tag="PW101_000454"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDL2512885.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            825..1079
                     /gene="purS"
                     /locus_tag="PW101_000455"
     CDS             825..1079
                     /gene="purS"
                     /locus_tag="PW101_000455"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDL2512886.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1081..1752
                     /gene="purQ"
                     /locus_tag="PW101_000456"
     CDS             1081..1752
                     /gene="purQ"
                     /locus_tag="PW101_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HDL2512887.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1753..3975
                     /gene="purL"
                     /locus_tag="PW101_000457"
     CDS             1753..3975
                     /gene="purL"
                     /locus_tag="PW101_000457"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDL2512888.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            3960..5399
                     /locus_tag="PW101_000458"
     CDS             3960..5399
                     /locus_tag="PW101_000458"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL2512889.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5422..6474
                     /locus_tag="PW101_000459"
     CDS             5422..6474
                     /locus_tag="PW101_000459"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDL2512890.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6471..7049
                     /gene="purN"
                     /locus_tag="PW101_000460"
     CDS             6471..7049
                     /gene="purN"
                     /locus_tag="PW101_000460"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDL2512891.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7046..8581
                     /gene="purH"
                     /locus_tag="PW101_000461"
     CDS             7046..8581
                     /gene="purH"
                     /locus_tag="PW101_000461"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDL2512892.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8597..9871
                     /gene="purD"
                     /locus_tag="PW101_000462"
     CDS             8597..9871
                     /gene="purD"
                     /locus_tag="PW101_000462"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDL2512893.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            9993..10907
                     /locus_tag="PW101_000463"
     CDS             9993..10907
                     /locus_tag="PW101_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2512894.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11040..12605)
                     /locus_tag="PW101_000464"
     CDS             complement(11040..12605)
                     /locus_tag="PW101_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2512895.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12598..14049)
                     /locus_tag="PW101_000465"
     CDS             complement(12598..14049)
                     /locus_tag="PW101_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2512896.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14063..15079)
                     /locus_tag="PW101_000466"
     CDS             complement(14063..15079)
                     /locus_tag="PW101_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2512897.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15299..16387
                     /locus_tag="PW101_000467"
     CDS             15299..16387
                     /locus_tag="PW101_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HDL2512898.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16441..17967
                     /locus_tag="PW101_000468"
     CDS             16441..17967
                     /locus_tag="PW101_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL2512899.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            17969..19174
                     /locus_tag="PW101_000469"
     CDS             17969..19174
                     /locus_tag="PW101_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2512900.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19431..20408
                     /gene="guaC"
                     /locus_tag="PW101_000470"
     CDS             19431..20408
                     /gene="guaC"
                     /locus_tag="PW101_000470"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDL2512901.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            20672..22096
                     /locus_tag="PW101_000471"
     CDS             20672..22096
                     /locus_tag="PW101_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL2512902.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            22557..23666
                     /gene="pdhA"
                     /locus_tag="PW101_000472"
     CDS             22557..23666
                     /gene="pdhA"
                     /locus_tag="PW101_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDL2512903.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            23669..24646
                     /locus_tag="PW101_000473"
     CDS             23669..24646
                     /locus_tag="PW101_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDL2512904.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         7994 a         4203 c         5580 g         6913 t
ORIGIN      
        1 ttgatttata aaaagatgat tgctaaaatt taaagtgaaa aaacgaacaa aaaacgatgt
       61 atttgctttt aatgtaaata aaaaggagag acgtacatgg agaagaaaga acttttgtat
      121 gaaggaaaag ccaaaaaaat atatcttact tctgatgaga acttgatttg gatagaatat
      181 ttagatcaag caactgcgtt gaatggcgcg aggaaagacc agataagtgg aaaaggagaa
      241 ttgaataatc aaatcacaag tgcaattttc cgttatctga ctcaaagagg aataaaaaat
      301 cattttattg aacaattgtc taaaaccgaa caattagcaa aaaaagtaca aattatccct
      361 ctggaagtgg ttatccgaaa tattgcagca ggaagtttct caaaacgtct aggtgttccc
      421 gaaggtaaag aattcatcaa accaatcatt gaattctact ataaggatga ccagttggat
      481 gatccattta tcaatgaaga gcatatctat ttcttacaac ttgcaaagcc ggaagaaatc
      541 agtgagctga aagcccaagc gttggctatt aatgaaattt tgaccgaatt atttgctaag
      601 gtcggtttgg ttttagtaga tttcaagcta gaatttggcc ggtcgaatgg acaaattttg
      661 cttgcggatg aagtaacacc ggatacttgc cgattatggg atgaacgtac gaatgaacat
      721 atggacaaag acgtttaccg cagagatcta ggtgatttga tcccagtcta tcaagaagta
      781 tataaccgtc tatcggaact aactgaagaa aaggagcgag tatgatgtat ttcgtaaaag
      841 tatatgtaac ctataaagat tcagtgttag atcctcaagg ggaggcagta aaaaaagcag
      901 tccatcgtat gggatatgaa acaatcgaag atatccgaat aggaaaatac tttgagatac
      961 aacttgctga tgcagaagaa ccaatcgata cagtgattga aacgatttgt gacaagctgc
     1021 ttgcaaatgt aaatatggaa acataccgat atgaaatcaa aaaaatggag gaagtataac
     1081 atgaaatttg cggtcatcgt ttttccaggt tcaaattgtg acgcagatat gttatgggca
     1141 gttcgcgaga tcatgggcgc tgatgccgaa tttgtacggc atgatgagtc ctctttagaa
     1201 ggatttgatg gggttttact gccgggagga ttctcctatg gtgactattt gagatgcggt
     1261 gcgattgctc ggttttctac aatcatggaa gaagtgattc gttttgctga agaagggaaa
     1321 ccagtattcg gtacatgcaa tggattccag atattgacag aagcaggact tttgccaggt
     1381 gtactgagac gaaatgagtc tttgcatttt atttgcaaaa ctgtaccatt gaaagtcaac
     1441 cccaagacgg cctttacttc agagtatgaa gccgacgaaa taatctatct tccagttgct
     1501 catggggaag gaaattattt ctgtgacgaa gaaacacttc aagaactaaa aaaaaataat
     1561 cagattgcct ttacttataa tgacaacatc aacggcagcg tggaaaacat tgcagggatt
     1621 acgaacaaag cagggaatgt gttaggaatg atgcctcatc cagaacgtgc agtagaagac
     1681 ttgctaggat cagaagatgg gaaacggttc tttgcttcca ttttaaaaaa cttcggaaag
     1741 gtgaccgtat agatgaaaac attgaaagaa ccgacagctg aagagatcaa gaaaaaaaga
     1801 atctatgccg aatggggatt gactgatgaa gaatatcgaa tgatcgaaga agatatctta
     1861 ggaagaatgc ctaattatac ggaaactgga ctgttttctg ttatgtggag cgaacattgt
     1921 tcctataaaa attctaaacc ggttctacgc aaatttccta ctagtggacc tcatgtgttg
     1981 caaggccctg gagaaggcgc aggcatcgtc gacatcggtg atggactagc agttgtcttc
     2041 aaagcagaaa gtcacaatca tccttcagcc gtggagcctt atgaaggagc tgcgacagga
     2101 gttggcggaa tcatcagaga tattttcagc atgggggctc gtccaattgc gttgctagat
     2161 tcattgaggt tcggcgaatt agataatcca agaacgaaat atttactaga agaagtagtt
     2221 gcaggtatcg gcggatacgg caattgtatc ggcataccga ctgtaggcgg cgaaattgca
     2281 tttgatcctt gttatgaagg aaacccatta gtcaatgcaa tgtgcgtagg tttgattgaa
     2341 cacaaacata tccagaaagg gcaagccgct ggcgtcggta attcgatcat gtatgtaggt
     2401 gcaaaaacag gtcgcgacgg tatccacgga gcaacttttg cctcagaaga atttaatcaa
     2461 gaagaagaac agcaacgttc tgctgtacaa gtaggcgatc cttttatgga aaagctgctg
     2521 cttgaagcgt gtttggaatt aatcttgcat cattcggatt ctctaatagg tatacaggat
     2581 atgggagcag caggacttgt ttcatctagt gcggaaatgg cttcaaaagc tggctcagga
     2641 cttatattga cattagatga agtcccacaa agagaaactg ggatgacacc ttatgaaatg
     2701 atgttatcgg aatcacaaga acggatgttg atttgtgtta aatcaggaga ggaagaaaaa
     2761 atccaagaac tatttcagaa gtatgatcta gatgcagtca ctatcggtaa agtaacagac
     2821 gacgggcaat atcggctata tcatcaagga gaagaagtag caaatctgcc agtcgatgca
     2881 ttagctgaag atgcaccagt ttatcataaa gaaatgaaag aaccagctcg aatcgtagaa
     2941 tttcagcagt tagctcctta tcagccagtt atcgaagaac ctggacaagt attgcttgat
     3001 ctgctgcagc aaccgacgat tgcttcaaaa cgttcaatct acgaaactta tgattcacaa
     3061 gtacaaacca atacagttgt ccgtccagga agcgatgctg ctgtgatgag ggttagaggg
     3121 acgaataaag cgttagctat gaccacagac tgcaacgcgc gctatctgta tttggatcca
     3181 aaaatcggag gacaaatagc agtggcagaa gctgctagaa atattattgc tagcggtggt
     3241 aagcccttgg cgatcaccga ctgcctgaac tatggttctc ctgataaacc agaagttttt
     3301 tgggaattat cgacttcagc tgacggaatt gctgctgcgt gtgaaacgtt aggaactcct
     3361 gttatttcag gaaatgtctc tttatataat gaaacggatg gtcaagcaat ctatccaaca
     3421 ccaatgattg gtatggtcgg attgattgag gatcataaac atatcactac tcaggaattc
     3481 aaaaaatcag gggatctaat ctatatatta ggtaagacgt ttgcagattt tgatggcagt
     3541 gaattacaaa agatgcagct tggaagaatc gaaggtgtga tccgaaactt tgatctatcc
     3601 attgaaaaga gaaaccaaga gttagtattg acagccatcc aaaacggatt gattgaaagt
     3661 gctcatgact gttcagaagg tggcttggca attgcactgg cagaatcagc tttcaaacat
     3721 cagctaggga tatctgtaca gtttgaattg tcctctgctc aattatttgc agaaactcaa
     3781 tcaagatttg tgttgacagt cgcaccggaa aataagacac gattcgaaga aatgatggga
     3841 gatgctgctg ttttagcagg taaggtgacg gatgaagcga taatcgaaat ttcagcaact
     3901 gacggtcaga tcaaaataga aactgcagta gcaagaaagt gctgggagga tgcaatcgta
     3961 tgtcttatga agtgaagagt ttaaatgaag aatgcgggat cttcggtgtt tggggtcatc
     4021 cagacgccgc tagtgtggca tacttcgggc tccatagtct tcagcatcga ggacaagaag
     4081 gtgcaggtat cgtgtccaat gatcgaggaa agcttaaggg gcatcgagat ttggggctgc
     4141 tttctgaggt attcaaagat caaagaaaat tgcagcagtt aacaggagaa gctgcaatcg
     4201 gacatgtacg ttatgcaaca gcaggcaatg gaagtgtgga caatattcag ccttttctat
     4261 tcaaatttca tgatcagtcg atcggcttag cgcataatgg taacttgatc aatgctcgtt
     4321 cccttagaaa agagttagag gaaaatgggg cgatcttcca ttctaactcc gatacggaga
     4381 tattgatgca tttgatccga cgaagtgaag aaacagattt tttaaataaa ttaaaaagtg
     4441 ctttgaatga agtcaaaggt ggctttgcct atttattaat gacagaaacg gcaatgattg
     4501 ccgctttgga cccaaatggt tttcgtccat tagcaatcgg acaaatgaaa aacggagcat
     4561 acgtgattgc ctcggaaact tgtgcattag aagttattgg ggcacgtttt ataagagatg
     4621 tccagcctgg tgagatagtg atcatcaatg acgaaggaat ccagattgat tgctttacga
     4681 aggacacgca gctttctatt tgttcgatgg aatatatcta ttttgccaga ccagattcaa
     4741 acatcgcagg gatcaatgtg catactgcac ggaaaaatat gggacggaat ttagcgaaag
     4801 aatcgccagt aaaagcagat atggtcgttg gcgtacctaa ttcttctttg tcagccgcaa
     4861 gcggttatgc ggaagctagt ggaatccctt atgaaatcgg attagtaaaa aatcaataca
     4921 ttgcccgaac atttatccag ccaacgcctg agctaagaga gcaaggtgtt cgaatgaaat
     4981 tatctgctgt acgaggagta gtggaaggta aaaaggtgat tatggtcgat gattcaattg
     5041 ttcggggtac gacaagcaga cggatcgtcc atcttttgaa agaagcaggg gcaaaagaag
     5101 tccatgtgag gattgcttct cctcctttga aatatccatg tttttacggg attgatattc
     5161 aaacgcgtaa agaactcatt gcggcaaatc acacgattga tgaaatcaaa gaatgtatcg
     5221 gtgcagattc tttaggtttt ttaagtgaag agggtctgat cgaatcgatc ggtctggaaa
     5281 aagatgctcc gtattcagga ttgtgtatgg cttatttcaa cggagattat ccgacgccgt
     5341 tatatgacta tgaagaaaaa tatcaggaat cactaaaaga aaaagtatcg ttcttttaaa
     5401 agcagaccaa gaagggagta tatgaattca atggcaaatg catacgcaaa agctggcgta
     5461 gatgtcgaag ctggatatga agtagtagaa cggatcaaga agcatgtcaa aaaaacggaa
     5521 cgtttaggta tgatgggcgc tataggagga ttcggcggtt gttttgattt atcggtcttt
     5581 caattaaaag agccagtcct tgtctcgggg accgatggcg tggggacgaa attagcagtg
     5641 gcaatcgaag cagacaaaca cgatacaatc gggatcgact gcgtagcgat gtgtgtcaat
     5701 gatatcttag cacaaggcgc agagccgtta tatttcttag actacttagc aacggggaaa
     5761 aatgatccgt tacgtttgga aaatgtagta aaaggtgtag cagatggatg tgttcaagga
     5821 aatatggcgc tgattggcgg agaaacagct gaaatgcctg gtatgtactc aggtaatgat
     5881 tatgatctag ccggatttgc tgtagggata gctgaaaaat ctcaattatt gacaggagaa
     5941 acgatcaacc caggagatgt gttgcttgga atagcatcta gcgggattca ttcaaatggg
     6001 tactctcttg ttcgcaaaat tttctttgag cagcagcagt tttctttatc atcaaaactg
     6061 ccagaattga aaaaaacact tggcgaagaa ttgcttgagc caacgaaaat ctatgtttct
     6121 tccttgcttc cgctgatcaa agaaaagaaa ataaaaggag ctgcacatat cactggtgga
     6181 gggtttgtag aaaatatccc gcgaatgtta ccagaatcta tggctgcaca aatcaaactt
     6241 ggctcatggc cgattcttcc aatattcggc tgtttacaaa aatatggtga aattcctgag
     6301 atggagatgt atgaaatctt caatatgggc atcggtatgg tcctggcagt ccaagaatcg
     6361 aaagtaaaag aaatagtaca cgaaattgag caagctggcg aaaaagttta tgaaatcggg
     6421 aaagtgatcg ccaaagaaaa acatcgtgtg gtgtttatag gagaagaacg atgagaatcg
     6481 ctgtttttgc ttctggaaac ggaagcaact ttcaagcgct agccgattat ctatcgaaaa
     6541 aaggattgga atcttcgatt gactggttgt tttgtgatca gcctgaagca tatgttttga
     6601 aacgtgcaac tgcgttgagt gtaccggcag attgtttttc acctaaagaa tttgattcaa
     6661 aaaaagagta tgaagaagca attttgcata aattgaaaga aaaaaagatc gatttgatcg
     6721 tattagctgg atatatgcga atcattggac cggtattgct gaaaaattat gataaaagaa
     6781 tcatcaatat ccatccgtct cttttacctg ccttccctgg tttgcatggc atacgtgatg
     6841 cctttgaagc tggagtaaaa gaaacgggtg tcacaattca ttatatcgac caaggtgtag
     6901 atactggacc aatcatccgg caggaaaaag tgagaattga acaggaggac acgttcgatt
     6961 ctttagaaga aaaaatccat agagtagagc atcgaatcta tccagaagtg attagtgaaa
     7021 ttattgaaaa tggaggctat ctgaaatgac aagagcattg atcagtgtat cagataaaac
     7081 tgggattgta gcattcgccc aaggtttgat tgaacaagga gtagaaatca tttcaacagg
     7141 aggaacaaag catgcgttgg ataaagctgg gatcaaaacg ctttcgatcg aagaagtgac
     7201 agggtttcct gaaatgatgg atggccgtgt caaaacactt catcctaaaa tccatggtgg
     7261 tcttttagga agacgcgatc tccctagcca tatggaggca atggaaacgg aaaatatcca
     7321 accaatcgac tttgtttgtg tcaatttata tcctttcaaa gaaacgatta tgaaaccaga
     7381 tgtcgaaata gcagaagcta ttgaaaacat tgatatcggt ggtcctagta tgctccgtag
     7441 tgcagcaaaa aaccatgaat ttgtcacctc tgttgttgat ccatctgact atgaagaagt
     7501 actttcagaa ttacgtcagt atgggcatac ttcactttct ttcagacaaa aactagcggc
     7561 aaaagtcttt cgtcatactg cggcatatga tgcactgatc gctcaatact taacggattg
     7621 ggtggaagaa aaagaaccgg aaaaattgac acttacgtat gaacgaaaac aaacgcttcg
     7681 ttacggtgaa aatagccatc aagccgctgc cttttatcag tccgctcttc tggaaaaata
     7741 ttcgatcgca gcagcaaaac aactccatgg gaaagaactt tcttataata atatccgaga
     7801 tgcagatgct gcgctacgga tcatgaagga atatgacgag ccaacggtag tcgccttgaa
     7861 acacatgaat ccttgtggta ttgggacagc atctacgatt ttagaagcgt gggaagcagc
     7921 gtatgaagca gatcctgttt caatctttgg tgggatcatc gttttgaatc gagaagtaga
     7981 tctgccaaca gcagaacaga tgcataagct gtttctagaa atcatcattg ccccttctta
     8041 tacacaagaa gctttggaat tactgcaaaa aaagaaaaac atccgattac tgacagtaga
     8101 ctttgcgcag gaagaaaatc aaaatcgaga agaaacggtc tcagtcttag gtggacttct
     8161 tgtacaagat caagatctgg caatagaaaa tgaagaagag tggaaagtcg tgacaaaacg
     8221 taagccgact ccagcagaag caaaagcatt agcgtttgct tggagagctg tcaaacatgt
     8281 gaaatctaat gcgatcgtac tggcaaacga acgtcagaca gtaggaatcg gagccgggca
     8341 gatgaatcga gtcggttcag taaaaattgc gattgaacaa gcgcaagaga aaatggaagg
     8401 tgcagtttta gctagcgatg cttattttcc aatggatgat tcggttgaat atgcagccaa
     8461 acatggtata aaagcaatca ttcagccagg aggaagcatc aaagaccaag attctattga
     8521 tatggcgaat aaatacgatc tagcaatggt ctttactgat gttcggcatt ttagacatta
     8581 gagaggagtc ggataaatga acattttggt gatcggtgca ggtggtcgtg aacatgcaat
     8641 aagtaaaaaa cttttagcca gtccgaaggt ggatacggtg tattgtgcgc caggaaatcc
     8701 ggggatgaca aaagatggta tcaattgtgt cgctatcagt gagcgagatc attcagcgtt
     8761 aatccatttt gttaagaaag aggctattga ttggagtatc gttggtcctg aagttccttt
     8821 gttaaatggg atcgtcgatg attttatggc ggaaggattg aaaatcttcg gtccgacaaa
     8881 agcagcagct ctgatcgaag gttcgaaaag ttttgctaaa caattaatgg atacttatca
     8941 aatcccgaca gctaaatcca gaagttttga atgttacgaa aaagcagcag cgtatatcaa
     9001 agaaaatggt gcgccaatcg tagtgaaagc ggacggctta gcagctggga aaggtgtagt
     9061 cgtggctacc acagtgaagg aagcgctgga tgcggcagaa caaatgttga accagcatcg
     9121 atttggggat agtagcaaaa aaatcgtcgt agaagagttt ttggcaggag aagaattctc
     9181 ccttttggcg tttgtccgta ataccaaggt gtatccgatg gtcatttcgc aagatcataa
     9241 gcgggcattt gataacgata aagggccaaa tacaggcgga atgggtgctt attcaccagt
     9301 accgcaaatt tcaaaaaaga tgattgaaaa agctgtggaa gaagttttaa aaccagcggc
     9361 tttgggaatg aaagaactag gacgctcttt cacgggtgtt ttatatgcgg gtttgattgc
     9421 tacgaaggaa ggaccaaaag tgatcgaatt caatgcgcgt ttcggtgatc ctgaaacgca
     9481 agtcgttctt tctcggttga agagtgactt tgcgcaagta atcgatgatt tattagagaa
     9541 tcgtacagta gagttaaagt ggcagcaaga aggatacaat gtaggcgttg tggttgcagc
     9601 tgccggttac cccgaagaat atgagacagg aatggttcta ccagattttc tggaagaaga
     9661 gctttctgtt tactatgctg gagtttcctc aaataacggc aaactagttg gagcaggagg
     9721 acgtctttat ttagtagaag catacggtga gacgatcaaa gaagcacaga aaaaaattta
     9781 caccgcgtta atggataaaa cgacagatgg aacattttat cgtacagata ttggagaaaa
     9841 agctgccaat agcgaactga tcgtgaagta aatcagctgc ttccatttat aaaaaataca
     9901 acaaacataa tcggaagcgc cgattttagt gaagcctttt cataaagagt gtggtacact
     9961 tttgttgtgt atataaataa ggaggagttc agatgaaaaa aattcaatta ggtacttctg
    10021 atcagaaagc agctgcagtg atcttaggct gtatgcgtat caacaatgcg gaagatccag
    10081 taaaagtgat tgagacagct tatgaaaacg gcattgattt ttttgaccat gcagatattt
    10141 atggaaacgg tgaatgtgaa gaaatctttg cagatgctct agcaaaaaca tcaatcaaac
    10201 gagaagatct ttttattcaa acaaaatgtg gaatcgttcc aggtgtgatg tttgattttt
    10261 ctaaaaaaca tatcattgaa tctgtagaaa aaagtttgac ccgtctgaaa atggattatg
    10321 tggatgcttt acttcttcat cgtccagata cattggtaga accagaggaa gtagctgaag
    10381 cgtttgatga attggaacgg tcaggaaaag ttcgttattt tggcgtcagc aatcaaaaac
    10441 caatgcaaat cgaattgctg aaaaaatatg ttcgtcaacc attagtagct aatcagctgc
    10501 aatttggctt gaaacatact ggaatgatcg atcaagggat tcatgtcaat atgacagatg
    10561 aagcaagtat cgatcatgac ggtagtgtac ttgattattc ccgattgcat gatatgacga
    10621 tccaagcatg gtcaccatac cagtatggat tttttgaagg tgtatttatc gggaacgaaa
    10681 aattccctga attaaaccaa acattagaag aactagcagc taaatatcaa acgacaccaa
    10741 ctggcttggc ttctgcttgg attttacgcc atccagccaa tatgcaagtg atcgccggta
    10801 cgatgaacgt agggcgaatc aaggaaatcg ctcaagcttc agaaattaat atgtctcggg
    10861 aagattggta cgaattgtac cgtgctgctg gaaacatttt accttaaaga ttgtcataag
    10921 caggtcaaaa gggctataaa ttaaaaagac agaataactt gaaagctgtt ttcagcttcg
    10981 agatattctg tcttttttta ttgcaaatgg aaagagtatc attgtacgga tttcgtttat
    11041 tagttatttt tgtttttaag gaaaagctgt gtccgatatt cttttggaga aatcccttgt
    11101 tttttcttaa ataaataact gaaatagtta gggtcattat aaccaatttc aaaagcaata
    11161 tctgaaatac gatagtctga ttgctgcaat aatttttttg ctaattcgat tctttgttct
    11221 gtcagataat cgatgaatgt ttggttaaac gattgagaaa aaatcgtact gaaatgagag
    11281 ggacttaatg caacttgttg agcgacccaa gttaaggaca tatctggatc ggtaaaattt
    11341 tctttgataa aatccaatgc atggttcaaa attggctgat atttgttcct tgaaggctgt
    11401 gagggcagag cagttaagaa agaaatgaac atataaactg tcgaacggta aagcgagtcg
    11461 tcggcgctga ttgctaagcg ttgactggtg gatgtgcaat gaaccatttc ttgcgggatt
    11521 ttcttttctt tcaggcagac atgctggagt agcttagtca agacaaataa acgatataat
    11581 cgtgttcgag ttggtgtgcc ttgtggtttc gtcaagtgtt cgatgtactt agatacttcg
    11641 tttttcgata atgaagacaa atcaagtgag aggtcaaagt aaaagacaga atcaactgga
    11701 agtttcgctt cttgaatcaa atcctcatag ctgataattt gttctttaag ggaactggcg
    11761 gtttcaagta tcatctgcgt gatctcaaaa gatgcaggta attcgctgat tctttctgcg
    11821 acaggaccaa tggcgacaga aaaattattt ttttgtccct tttctaattc atggaccaac
    11881 gtttgggcta cctgataact ggtctcgatc acattttctt caagagaatc aactattaaa
    11941 aatttgatga atcgagagga aacagcagag aagatcactt gttgattctc cctaaataaa
    12001 aacttcaaat aatcactgag atgagcgtaa tcttcaaagt ctgtactgac ttggtttgct
    12061 gctaataata cggtcatttt tttccctgct aaagagcgat tgaaagattc agcaagtttc
    12121 aatgtttcac tgatcgtaag tgagcctttg aacaaaccgt tcaagaaatg attttttttc
    12181 aattcgaaaa caagatcaga agaagtgtgc gtttcttttt gctgcaaatc taaagcagtt
    12241 actgcttttt ctattgcttg tttcaattcg ctacttttaa tcggtttaag cagataatca
    12301 tctgcctgta cctggattgc tcctttcata taatcgaaat cgtcgaatcc actaataaat
    12361 atgagacgag tccaaggaag tagtttcctt gcttcttttg aaaaagtcaa cccgtccatg
    12421 aaaggcattt ggatatcagt cagaatgatg tcgggctgta agtcgataat tgaagacagt
    12481 gccatctcac catctccagc ttcgccgaca taagtgattg gataataaga accaaggaca
    12541 gttatttgtt cccgcaagtt attgcgaatg aggtattcgt cttcgatgat aaaaacttta
    12601 tacatgtttg tttcctccaa ttgttttggg agatagaggg atcatcagtg tgacagttgt
    12661 tccttctcgg tatttgctgt caatcgtgat aggtttttga tcatttccgt aataaagaag
    12721 gagacgcttg tttacgttgt aaagtccgta gccgatactg aaatctgaat cgatcccttt
    12781 ggctaactct ttacgtattt ctgccagttt ttctgcaggg atgccaatcc cgttgtctat
    12841 aactttgaat tggatcatct catcaatttt gcatgtggta atacgaataa atcctttgtg
    12901 acggacgtac ttcgttccat ggtatacagc attttcaatc aatggctgca agatcatttt
    12961 taaaatagaa acaggaaaaa gtgtttctgg tatatcgatc ttatagtcaa gcatatggcc
    13021 gtatcttatt tttaagatga ctagataatc attgatatgg ctaatttctt tttcaagagg
    13081 aatccagtct tgtcctttgc ttaaagacgt acgaaaaaag ccggaaagtg catgggtcat
    13141 agtcagagct tcttcaaagc agtcttgttc gatcaaagaa ataatggcat ccaaactgtt
    13201 ataaataaaa tggggagtaa tctgagcttg caatacacga acttcacttt gtgctaaatg
    13261 gtattgtttc aaagcgttct cccgtaaaga ctggtttaga tcttccgcca ttttattcat
    13321 gctagcggta agtgtttgta attccagtgt ttccggcagg gttaaacgaa agccaaggtt
    13381 ccctgaagaa agttgttctg acatctcgat cagctgattg atcggttctt ggacctttct
    13441 ctctaaaaag cgacgattct ttttcgcata gaaaaaaatg gtactaagaa ctccaagttc
    13501 cactattgtt aatagaatga gcgagcggac aagtgtcgta ttttttttgc ttgctaagtc
    13561 gatctccaca cgaacaaaat cttgtaagat atctgacagc agttgagtga cagaatctac
    13621 ctgattgatg atctcttcat tggtatccat aaaatctttt gccaagatat tattgatcag
    13681 ctcgttttga taatcttcca gtgtatcgat cgtacgtagc gatacatcta acgtactaat
    13741 ttctttaagt gtatttgtat tatttttgat ctctcgaata tcattcctaa gttcttctag
    13801 gatattttcc tcattgaact catctacggt tacttggcca gaaatcaagt cccacatact
    13861 ttccaaaact ttttgatcaa ccttcaaagc aattgcatct gctttttgga tatttgcgat
    13921 cgtctgttga taaatcaaaa gctgttgcga atataacagt atgctcagta gcaaagggat
    13981 aacagcaaat agaagcatgg ctttgtttgc ttgggcaaaa atctgtttta tgcttaaatt
    14041 ttttgtcatg atacttctcc agtcaataat ttctggttgg gatgttagct atattctgct
    14101 gggaaaagac agtctcggaa ataaaaatat ctttaggtaa tgtttttcct gtttgaatat
    14161 ttccaaaata acgagtaacg gtattggcta cataccagcc gacatcaggt ttacactcga
    14221 ctacacaatt gacgattcct gagcgtagct tctcgatcat atcttcttgt ccatcaatag
    14281 ttacaatgat caaatcttct ccaggtttga ttgatgtttc ttttatcgca gctaaagcac
    14341 cgtgagtcat ttcatcacta tggctgtata atacatcgat ttcggataaa tcatttgttt
    14401 ctaaatatcg tttcatctgg tcttttcctt tcatccgaat aaaatcaccg acaattcggt
    14461 gaacaatagt catcggaggt ttatggtcga tggttccatc tttttttagg gttggtttac
    14521 gattgattga ttccaaaaat ccttcggagc gtagcttggt cggtgtggag ttctctgaac
    14581 cagcaagttc taggattctg atttcctgtt tctttgaatt tgtgaagtga ttgctgacaa
    14641 ataaacccgc ccgatttcct tctgctttga aactggggcc aatgtgggtg acgaaaagct
    14701 cgggatcatt cgtttgaata tgtctgtcga caagtatgac agggatccct gctcgctttg
    14761 cttcttttaa tacagagtcc cacccggatt cttgcaaagg agtaaaaatg atcatatcca
    14821 ctttataggc aatgaacgtc cgtatatctt ggatttgccg ttcctgattc atgtagccat
    14881 ttcggtagag aacttggtaa tcttcttttt caagcgcagt gataattgat tcagtatgct
    14941 ttttccgcca gctactctcc gttcctgatt gcgaaaaacc aacgactagt gggtcaggtg
    15001 cttcctttgt attaggtgta caccccatca tttggtaagc gattacaaca agggctaaaa
    15061 aaaataaaac acgtttcact ttaaatcact ccatctggat ttgttagtac tatcttatca
    15121 gataaaaggg aaaatcaaag tcaaaaggaa tcaatcgaaa aaaatccaag aaacttaaaa
    15181 aaatccaaga caaaaaaagt acacctaaaa aatttaaagt aaatcccata aaaaaatcaa
    15241 tgttaacgct ttctttatta gctatactcg gtgtatcaat tgaaatgggg gataaaaaat
    15301 gaaaaacaaa tttagaaaga ttgttgggac tatcgctatt ttgaccgcaa gcattctatt
    15361 aagtgcgtgt ggcaattcag gatctgcaga tgatagtacg gggtatgtag gaatcgctat
    15421 gccgaccaaa tcagcagaaa gatggatcgc tgatgggaac aacatggtct cggaattaga
    15481 aaaactgggt tataaaacag atcttcaata tggagaagat aaagtagaaa atcaagtagc
    15541 acaaatagaa aatatgatta caaaaggggt agatacactt gtgattgctt ctatcgatgg
    15601 ttcggcttta actgatgtgt tagctaaagc aaaagaagca gatatcaagg tgatcgccta
    15661 tgatcgttta ctgatgaatt cagaaaatgt cgattattac gcgacttttg ataattttgg
    15721 agtaggtgta tcgcaagcag cttatattga agagcactta ggattgaaag aaggaaaagg
    15781 accattcacg attgaattat ttggtggctc gcctgacgac aacaacgcac taatcaatta
    15841 caacggagtg atgtctgttt tacaaccata tatggacaac ggtcagttgg tcgtgccatc
    15901 tggacagacg agtttcagtc aaatcgctac gcttcgctgg gatggttcta cggcgcaagc
    15961 aaggatggat aatctgttaa gcgcaaatta tactgaccaa acattggatg ctgttttgtc
    16021 gccatatgat ccaataagtt taggaatcat ttcgtcatta aaaggcgtgg ggtatgggag
    16081 tgaaagtaaa cctttacctg tgattactgg acaagatgcg acagttgctg gcgttaaatc
    16141 gatcattgcc ggggaacaaa cacaaacgat ctttaaagac acacgtatct tagcaaaaaa
    16201 tacgattgaa atgatcaaag caatcagtga tggagaagag gttccggtca atgatactga
    16261 aacttatgat aatggggtga aaactgtccc tacgtattta gcaaatacag tatctgtcga
    16321 taaagataat tatcaagcag agctgatcga caccgattat tataaagaat ccgatctgaa
    16381 aaattaagag gaaccaaaca aggaactaaa gagatctaag tcagagagag gataagaatt
    16441 atggcggatt gtattttaaa aatggcagat atcaccaaaa aattttctgg aatccgagcg
    16501 ttagattgcg tgaatctgat gattgaacgt ggggaaatcc atgcattgtg cggagaaaac
    16561 ggagcgggaa aatcaacatt gatgaatgtc ttgagcggct tgtatccata tggctcctat
    16621 gacggggaaa tcttttatga tggaaagctg tgtcaattta aagatttgaa agatagtgaa
    16681 gaaaaaggca tcgtcatcat tcatcaagaa ttagcactaa gtccttatct ttcaataaaa
    16741 gagaatattt ttctcggaaa tgaacaagca gaaagaggcg tgattgattg ggaactgaca
    16801 gaaaagaaaa catatgaact gctgaataaa gtcggtttgt ctatcgaccc gaatcttcta
    16861 gtatcccaga ttggcgtagg gcaacagcaa ctagttgaaa tagctaaagc attatcaaaa
    16921 tccgtccgtc tgttgatttt ggatgagcct accgctgcat tgaatgaaga agaaagcgcg
    16981 aatttgctga gattgatccg agagttgaaa gagcaaggtg taacctccat cattatttca
    17041 cataagctga atgaaatcgt ggatatcgca gatcgaatca ccattttacg ggatggacag
    17101 acgattgaga cactagaaaa tgaaatgatc agcgaagagc atattattcg cggaatggtg
    17161 ggcagagatt tagttgatcg ttatcctgaa agaaaaccgg atattggaga agtttatttt
    17221 aaagtagaag actggacggt ctatcaccct cttgatcacg agcgaatcat aaacgaccac
    17281 atcaacttgc atttgaaaaa aggagagatt gtaggaatag ctggactgat gggagcgggc
    17341 agaactgaat tcgctatgag tctatttgga cattcttatg gaagcaagat atccggacgt
    17401 atattaaagg aaggggcaga gatatcagtt aagaccgttc cttcagccat tgcaaacgga
    17461 ctcgcttatg tctcggagga ccgaaaatct ttaggactga atctgctgat ggatattcgg
    17521 gaaaacgtga cgctgagcag tttggataaa ataagtcaaa aaggagtatt agataaagaa
    17581 aaagaagtaa ctgaggcaga aaatttccgg aaaaagatgc gtataaaaac aaactccatc
    17641 catcaaattg tcagtagtct tagtggtggg aatcaacaaa aagtcgtttt atccaagtgg
    17701 ctgatgacac aacccgatat tctttttttg gatgagccaa cccgtgggat agatgttggg
    17761 gcaaaatatg agatctacac gattatcgaa gaaatggcag caaatggcaa agctgtctgt
    17821 cttatctctt ctgaacttcc tgaaatatta gggatgtgtg atcgaatcta tacgatgaac
    17881 gaaggacgta ttacaggaga agtctcgcga gaagatgcag atcaggaagt cttgatgaaa
    17941 ttaatgacga aagaagaggt gggttaagtt ggaacaggca aaaaatctta cggataaaaa
    18001 agagaaaaaa ctaaatttga aaagtagagc agctgatctt tttggtcgct acagcatggt
    18061 gatcatttta gcgggactac ttattgcgtt tcaattgatg acggatggta ttttctggcg
    18121 tccattgaat ataactaacc ttgttctgca aaatagccat atattagtac tagctgcagg
    18181 gatgttatta gttgtgctct taggtcatgt ggatttgtcg gtagggtcag tgatggcgtt
    18241 tgttggtgca attgcgggca tgatgatggt caatcgccaa atgaacccgt ggatgacagt
    18301 tatcgcatgt cttgcagtag gcgggctgat cggcgcatgg caaggttttt gggtagctta
    18361 tgcagggata ccggcgttta tcgtgacttt agcaggattg ctgatgtttc gtggactgac
    18421 acaagttgtt ctaggaggcc aatctctagc accttttcca gctgttttcc aaaaaatgtc
    18481 tacgggttat ttaccagact taagcgaagg aagcagcctt cacttcttga cattgatttt
    18541 aggaatgatc atcacgatcg tgttagttgt tggtcaatgg cgaacaagaa atcgtcggaa
    18601 aaatcattta tttgaagtgg aatctccaac agctttcttg gtaaaggcgg tatttacagc
    18661 agtcgttgta ctaggaataa cttatatctt tgcctcttat caagggtatc cgattatttt
    18721 gatcattctt ggtttgatcg ttgcagcata tgggtttttg acaaataaaa cagtagcagg
    18781 tcgacagatt tatgcgacgg gcggaaatcg aaaagcggct gaactatcag ggatcaaaac
    18841 aaagcgaatc acgttctggg tattcgtcaa catgggagtc atggcagctt tagctggatt
    18901 agttttagct gctcgattga atgcggcaac acctcaagca ggtacatcga tggaactgga
    18961 tgcaatggcg gcagtatatt ttggtggtgc ttcgacttcc ggcggcattg gaacaatcat
    19021 gggtgcgatt gtcggtggct tggtcatggg tgtattgaac aatggcatgt cgattttagg
    19081 cgtcggggta gactggcagc aggcaatcaa aggattgatc ttgcttttag ccgttgtgct
    19141 agacttgtat aataagaaaa aacgaacaac gtaggagaac gccggttatt cagaaaaaag
    19201 actgtgacaa gacatttgtc acagtctttt tttcgctaaa agcgaacatt cattgtttcg
    19261 tttgtataga ctaaaaataa tatatgtttt tttaccattc gtataaattc tctttttgct
    19321 cttgattttt ttgcttaatt gagttaaatt acatagggcg ctaaaaaaag ccttgattaa
    19381 attaataatg ttcgtgtttt gctggatgtg aacatcagga ggataatgta atgaaagtat
    19441 ttgattacga agatattcaa ttaattccta ataaatgtat cgtaaatagc cgttctgaat
    19501 gtgatacaac ggtcacatta ggtaaacata cattcaaaat gccagtagtg cctgccaaca
    19561 tgcagacgat tattgatgaa acaatcgcag aattcttagc ggaaaatggt tatttctata
    19621 tcatgcatcg gtttgatgaa gcagctcgaa ttccgtttat caaaaaaatg aaaaagcgtg
    19681 gattgattac ctcgatcagt gttggcgtca agaaagagga atattccttt atagagaagt
    19741 tggcagaaga atcgctaaat cctgattata tcactatcga catcgcgcat ggacatgcta
    19801 actcagttat tgatatgatt cagcatatta aaaaatattt gcctgaaact tttgtgattg
    19861 ccggtaatgt tggtacaccg gaagccgtcc gtgaattgga aaatgcgggc gcagatgcaa
    19921 caaaagtcgg gattggtcca ggaaaagtat gtattacaaa aatcaagaca ggctttggga
    19981 ctggaggctg gcaactggca gctcttagat ggtgtgcgaa agcagctcgc aagccgatca
    20041 tcgctgacgg tgggatcaga acacatggcg atattgcaaa atctgtccgt tttggtgcga
    20101 caatggtcat gattggttca ttgtttgccg gtcacgagga atctcctggc gaaacaaaag
    20161 tcgaaaatgg aatcgtgtat aaagaatact ttggttctgc ttcagaattc caaaaaggcg
    20221 aaaaacgaaa tgttgaagga aagaaaatct ggatccaaca caaaggatct ttgaaagata
    20281 cactagtgga aatgcaacaa gaccttcaat catctatttc ttatgctgga ggaagagatc
    20341 tggaagccat tcgaaaagtt gactatgtca tcgtgaaaaa ttcgattttt aatggagaca
    20401 caatctaaaa aataaataaa atattaggat gttgtgaaaa agttgtcctg cattaagtaa
    20461 taagaacagc caaacaaaaa tagttcttta tatgttttgt ttggctggaa ttattatcgc
    20521 agatacaagc ttttgaacat cattattcag aataagggac tgtgataaga tttttgtcac
    20581 agtcccttat gttttgcaaa aaaagttttt cttttttctc gtaaattcgg taaaatcaaa
    20641 agaaaaaata gacaatagaa aagagaagct tatgtgggaa aaagtagaga aaaaacaagg
    20701 accgatttat cgtcaaatta tggaacagat tatgagagaa atcgaaaacg gaaggatcaa
    20761 tcctggaagt caacttgaat cagaacgaaa actagggcaa ctttttggtg tgaatcgttc
    20821 caccgttgtc catgcattgg aagagcttag ggagcttggg gtactaacga gcaaacgtgg
    20881 cagcggatgg tttgttaatc agactgaatg ggggaaattc gctgttccaa gaatcgattg
    20941 gcggcaaatg atctctcctc gttatgagca gacggactgg tatgagcaaa aaataagaga
    21001 agcaaagcag atccaaaaaa catcgtttct tgatttatac gctagcgaga tgccagatga
    21061 gcttttgccg aattttgagt ttccttctta ttctcttgaa gaaatcttag cggaagaaaa
    21121 agaaatggat cttttaggct atactccgtt acgtaacaaa atcaaacggt atttggaaag
    21181 taaatttgaa ttggcatttt cccccaacca gcttcttgtg actggtggcg gtcagcaagc
    21241 aatctttttg attttacaaa cgattctttc agctggagag gggatagctg tagagtcacc
    21301 ttcttttttt taccgattac ctcttttcaa agcttcagga acgcgtttat ttgggatacc
    21361 gatggatgaa gaagggatcg acttagaaaa actgaaagaa tcgatccata aaaataaatt
    21421 gaaagcagtg cttatcaacc cgaattttca aaaccctaca ggaaaagtga tgagtcaaaa
    21481 acgacgagaa aagctagtag aattgtgtcg acagtatcgg cttccaatta ttgaagacga
    21541 tgtatttacg gatctttcat ttctaaacca agaagaagtc cttcctatac gaacgattga
    21601 tccggataat gtgttatacg tcggttcgtt atctagggtt ttaggaaaaa caacgaaaat
    21661 cggttgggtg ataggacctg agacttttat ctcgcaacta gctcaagcgc aagaaatgat
    21721 ggagttttcg atgaatatcc ttacacaaat cacagtggct aatgtattta atgaagcaat
    21781 tgatttgaaa atgcaagatg taagaaaaaa gttgaaagaa aaaagccgac tgataagcgc
    21841 ttggggagat tcacaatcgt ttttcaagct tcattcaatt ttaggtggtt actatgcctg
    21901 gattacttgg gatgggaaaa aactagaccc cgaactagca gaaaaagtag tagatatggg
    21961 tcttggaatt gcaccaagtc tactttttgg gcaagatata aatggaatca gaatcaatta
    22021 cagtaggctg gatgaaaacc aaataccgtt gttttattca aagatgaaaa ttctagaaaa
    22081 gtggttaaca gaataatttc acaaagtaaa tagattaaag aatataacag cttggattta
    22141 aatttcaagc tgtttctttt ttttcggaaa aagaatacga tataaagaaa ctaagaaaaa
    22201 accattattt gtacgtccgt tcacgatgtg taaataaaat aaatgtttgc aacaaaactg
    22261 tataacacca acgtttttaa atagaaacag atggttgtaa gtttgattag aaaatagaaa
    22321 aaatgcagat gaaatataaa acgctttcct tattgtgatt ttattcacta atgctgttaa
    22381 tatgcggatt ggcataattt cctttcatcg tgaaaatttg tgcttttagc ttgtatttct
    22441 cataaatcca tgataattta ttaatgaaga cggttaaata gtgatacagt tttattcact
    22501 gtttcaatcg cagaatgaag agattttctt atagatgaga gaggaatgtg tgacaaatgg
    22561 caaacaaaaa agcaccgatc gacttccaag cactgctaga tgaagtcaat gcagatttcc
    22621 cagtatatca agcacttgat caagatggaa acgtagttaa caaagattta gtacctgatt
    22681 tatccgatga tgaactcgtt gaattaatga ctcatatggt atggtctcgt gtattagacc
    22741 aacgttcaac agcgctgaac cgccaaggac gcctaggctt ttttgcacca actgctggac
    22801 aagaggcaag tcagctggct agtgcttacg catttgataa agaagatgtt cttcttccag
    22861 gttatcggga tgtacctcaa ctagtaaaac atggtttacc tttatctcaa gcttttttat
    22921 ggtctcgagg acatgctgca ggtaattctt atccggaaga attgaaagct ctaccaccac
    22981 aaattatcat cggtgcacaa tatgtacaag cagccggtgt tgcattaggt ttgaagaaac
    23041 gaaacaagaa aaatgtcgtc ttcacttata ctggtgatgg cggttcatca caaggtgatt
    23101 tttacgaagc aatcaatttc gctggcgctt accatgcaaa tgccgtattt tatatccaaa
    23161 acaacgggta tgcgatctct acacctcgtg aaaaacaaac tgcagcaaaa acacttgcac
    23221 aaaaagctgt cgcagctggg attcctggta ttcaagtcga tggaatggat ccgcttgcag
    23281 tatatactgt atctaaaatg gcacgggaat gggcgatcag tggaaatgga ccagtcctaa
    23341 tcgaaacact gacttatcgt tatggaccac atacactatc tggtgacgat ccaacccgct
    23401 accgtacaaa agacacagat gacgattggc agaaaaaaga tccattgatc cgcttccgta
    23461 aatatttgac ggaaaaagga ctttggtctg aagaaaaaga agaacaagtc atcgaagcaa
    23521 ctaaagaaga aatcaaagca gctattgctg aagccgacaa agtaccaaaa caaaaggttt
    23581 cagatttctt gaaaaacatg ttcgaagtat ctccacaatc aatcaaagaa caaattgcaa
    23641 tttatgaagc gaaggagtcg aaataatcat ggcacaaaaa acaatgatcc aagcaatcac
    23701 agatgcctta gcacttgaac tagaaaatga cgagaatgtc gttgtttttg gtgaagatgt
    23761 tgggaaaaac ggaggagttt tccgggcaac tgaaggattg caggaaaagt tcggagaaga
    23821 ccgagtattc gatactcctt tagctgaatc tggaatcgca ggattgagtt tcggtcttgc
    23881 gttagaagga tttcgtccag ttcctgaaat ccaattcttt ggttttattt tcgaagcaat
    23941 ggacgaagta gtagctcaaa tggctcgtac aagatatcga atgagcggca ctagaaatct
    24001 acccatcaca attcgttcgc catttggtgg cggtgttcat acaccagaat tgcattcgga
    24061 taatttagaa ggtttgatcg ctcagtctcc tggtattcga gtagtgattc cttccaaccc
    24121 ttatgatgca aaaggactat tgatttcttc tattagaagt aacgatccgg ttgttttcct
    24181 agaacacatg aaactatatc gctcattccg agaagaagtg ccggatgaag cttacgaagt
    24241 tcctttggat aaagcagcag ttactcgtga gggaacagat gtatctatta tcacatacgg
    24301 agcaatggtg cgtgaagcaa tcaaagcagc agacaatctt gcaaaagaaa atatatctgt
    24361 tgaaatcatt gatcttcgta cagttgcgcc attagatgta gaaacgatca ttcaatccgt
    24421 ggaaaaaact ggtcgcgtag ttgttgtcca agaagcacaa cgacaagcgg gcgttgccgc
    24481 gcaagtagtt tctgagatct cagaacgcgc tatcctttca ttagaagccc caatcggacg
    24541 agtttctgca cctgatacag tcttcccatt tggacaagca gaaaatgtct ggttgccaaa
    24601 tgcaaaagat atcgaagaca aagtaaaaga aatcgcggaa ttttaagaaa agaagggacg
    24661 aaacaaagtc atggcttatc aatttaaatt
//
LOCUS       DANKHL010000016        23983 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000016 DANKHL010000000
VERSION     DANKHL010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.018"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            42..674
                     /locus_tag="PW101_000474"
     CDS             42..674
                     /locus_tag="PW101_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512905.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(808..933)
                     /locus_tag="PW101_000475"
     CDS             complement(808..933)
                     /locus_tag="PW101_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2512906.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1067..1960)
                     /locus_tag="PW101_000476"
     CDS             complement(1067..1960)
                     /locus_tag="PW101_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HDL2512907.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2142..3068)
                     /locus_tag="PW101_000477"
     CDS             complement(2142..3068)
                     /locus_tag="PW101_000477"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDL2512908.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3153..3914)
                     /gene="pflA"
                     /locus_tag="PW101_000478"
     CDS             complement(3153..3914)
                     /gene="pflA"
                     /locus_tag="PW101_000478"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDL2512909.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4158..6389)
                     /gene="pflB"
                     /locus_tag="PW101_000479"
     CDS             complement(4158..6389)
                     /gene="pflB"
                     /locus_tag="PW101_000479"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDL2512910.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6666..9116)
                     /gene="parC"
                     /locus_tag="PW101_000480"
     CDS             complement(6666..9116)
                     /gene="parC"
                     /locus_tag="PW101_000480"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDL2512911.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9128..11173)
                     /gene="parE"
                     /locus_tag="PW101_000481"
     CDS             complement(9128..11173)
                     /gene="parE"
                     /locus_tag="PW101_000481"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL2512912.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11339..11773)
                     /locus_tag="PW101_000482"
     CDS             complement(11339..11773)
                     /locus_tag="PW101_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDL2512913.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11900..12538
                     /gene="plsY"
                     /locus_tag="PW101_000483"
     CDS             11900..12538
                     /gene="plsY"
                     /locus_tag="PW101_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDL2512914.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12670..13545)
                     /locus_tag="PW101_000484"
     CDS             complement(12670..13545)
                     /locus_tag="PW101_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDL2512915.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13627..14418)
                     /gene="codY"
                     /locus_tag="PW101_000485"
     CDS             complement(13627..14418)
                     /gene="codY"
                     /locus_tag="PW101_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDL2512916.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14436..15836)
                     /gene="hslU"
                     /locus_tag="PW101_000486"
     CDS             complement(14436..15836)
                     /gene="hslU"
                     /locus_tag="PW101_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDL2512917.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15850..16398)
                     /gene="hslV"
                     /locus_tag="PW101_000487"
     CDS             complement(15850..16398)
                     /gene="hslV"
                     /locus_tag="PW101_000487"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HDL2512918.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16435..17340)
                     /gene="xerC"
                     /locus_tag="PW101_000488"
     CDS             complement(16435..17340)
                     /gene="xerC"
                     /locus_tag="PW101_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDL2512919.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17538..18914)
                     /gene="trmFO"
                     /locus_tag="PW101_000489"
     CDS             complement(17538..18914)
                     /gene="trmFO"
                     /locus_tag="PW101_000489"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HDL2512920.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18883..20961)
                     /gene="topA"
                     /locus_tag="PW101_000490"
     CDS             complement(18883..20961)
                     /gene="topA"
                     /locus_tag="PW101_000490"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDL2512921.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21083..21940)
                     /gene="dprA"
                     /locus_tag="PW101_000491"
     CDS             complement(21083..21940)
                     /gene="dprA"
                     /locus_tag="PW101_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDL2512922.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21996..22763)
                     /locus_tag="PW101_000492"
     CDS             complement(21996..22763)
                     /locus_tag="PW101_000492"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDL2512923.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22756..23616)
                     /gene="ylqF"
                     /locus_tag="PW101_000493"
     CDS             complement(22756..23616)
                     /gene="ylqF"
                     /locus_tag="PW101_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDL2512924.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         6262 a         5151 c         4119 g         8451 t
ORIGIN      
        1 tattatactt tatatactta tcaatttaat ttggagatat tatggaaatt caacgtagaa
       61 ccactaattt tttacattta ggctcttttt ttgcaacttt gacgatctta ttttttctat
      121 gttattcata ccttaagacc tcacatgcgc cttctagtgc attaggctgg ttgcttttgg
      181 atcattctga tatcacagaa gccgaagaaa atccgttttt tttgattttg tcttctcttt
      241 gcggcttact gttttctgtt tatttcggtg cctatttttt tcatcgacat cctggtacgt
      301 taaacgaagc ttcaagcgta ttgattgcat ttaaaacgtt ttctttttta gcaattcttt
      361 tttattcgtt taatcacaat acgcttttct tctttatcat gaatggttta gtcgtgattt
      421 tatccatttt tacttatttt attttttcac tcatttttag ccaattgaaa tgtccgctct
      481 tctttcgatt agttcctgtc acctgtttta tttacagctt gttttccttt tttgtccttg
      541 ctttaatgcc actgaccaac ccttcgacga tccaagtcgc aggaaatctg ttggatatgc
      601 tcttcatcgt cagtctaggt gtatttatgt ggcaaagaaa aaacaaacaa aataaaaagc
      661 attttgaagc ctgactatta cgacttcata aatgcttttt tagttttaaa cacttcaatc
      721 taaaagaata acccaagatt ggataaatta ttaatgactc gccattcgtt tcttagtgat
      781 tctggattat caaatggact tcctaatcta atcagctagt tcttcccata aaatgccata
      841 cttgactgaa tcataatatt gattatttaa aaatctaact tttcttatca ctacttcctt
      901 tgtcattcca catttctcac catttttttg cattcctttg tttccagacc atgtagtgaa
      961 tctaataggt ggtagatagg caaaatactc aaataattct tttaaccata aaggaagaga
     1021 aaggctcctc ctgcaagagt cagtataatt tttgcttact gctctcttag gttttactca
     1081 ctaaaactgt tccatattct ttgatataag aaaatgcatg cggtataaat ttcttgctca
     1141 gctcagaaaa gatttgtcgt ttaagaaagg ccatttcctc cattgtcaat gaaggatcca
     1201 atgttggtaa ttcaatctca aaattgtttt tttcgacaat aggtaattct aatgtgaatg
     1261 attcttcttc ctttatagta ttcgtcacaa gtagagactg atggaaaata ctaggccggc
     1321 gatttttata tttattctta cgtatcctgt caacgataac ttctatttga tcaaaaaagt
     1381 acgattcatt tacgtctcca ataagttcgg ctaattcttt gaatgccttc gttacaggaa
     1441 gctgattctt ctgtacaaaa aagtaattag ccaaagtaag cgggaagtgt tctatagagg
     1501 aaagtgtgat aaaacggtac ttctcatttc ctgcagttgt cctcagcaat tctgtctctc
     1561 ggctgtttct tactccataa aaatacgctt ctttccgttc atcttcgaaa caatacgccc
     1621 aaataacttc tcccatcgct aatttttgtt catcaacaga gagtctcttc aattgttcag
     1681 ctatggtttc agcagccgaa gtggcttgtt gcaagtaatc tttaggatca aatattggaa
     1741 agttcaaatg atatcttcgt tcttccctct tgattaatcc tgcatcgatc aataaatcaa
     1801 gaaaatggga aaatttattt tgcgaaaatt cttttttcaa ttctcttaga atcgtttctt
     1861 gttctcgatg ttcatataga taatcggtca atgaagaaaa aagcggatca tcggtcagtt
     1921 tttgataaaa gttttcctta ttttttgaat aatagttcat atatgttctc ttttctatcc
     1981 gttttatttt acttaaaagg cttttacgta aaaaggagga agaacaagag tcataattga
     2041 ctgttattct cctccatttc ttcggccttt agtatgttct atgtttcttt ttatatcaat
     2101 cgcttatcca ttcattttga atgcttcata ctaccatttg attagctaaa tacagccgtc
     2161 aattgaggta cgacttgttt tttacgtgaa acgacacctt taagcaaagc acggttgttg
     2221 gtcaaagttg tttggaaagc ttcttctact ttttcttttc ctttaccagc aacaagtaat
     2281 tctgaatcac tgtctaagat gtttgtgaca actaatacga acagatcgta gccatttgct
     2341 ttcatttcat cttccattgc tgcttctagc tctgcttgtc gatccatcac ttcattaagg
     2401 tccactgtat tgacttgagc gatacgaacg ttggaactgc ccattgggaa actttttgca
     2461 tccaaatcaa gaagaaccgc agctgattta tcacttagat ttgttcctgc tttcaacatt
     2521 tcaagtccat aagtgtttag atcgacttca gcgattgctg caagttcttt tgcagctgct
     2581 tcatcttcag gcgtacatgt tggtgatttg aacaacaatg tatctgaaat gatagctgag
     2641 agcatgattc ctgcgatttc tttaggaatt gttacttggt tttctttata tagttttaaa
     2701 ataatcgtac ttgtacatcc aactggttct gcacgatagt acaatggatt agctgtttca
     2761 aagtttgcaa tacgatggtg gtctactaca gctaaaatat ttacttcgct gatatctgaa
     2821 atactttgtt ggaattcatt atgatccaca agcatgacat ctgcattttc tgctttagct
     2881 gactctacta ctcgtggtgc agatagctga aagtggtcta atgcgtattg tgtttcttca
     2941 ttaggtgtac ctagagcaac tgcttctgtt tctttaccta attcattttg caagtgtgaa
     3001 aacgcaatgg ctgccccgat tgcatctgta tctggatttt ggtgaccaaa aactaaaatt
     3061 tttgacatgt cattcgctcc tttgtgattt ctcctattta ataatagcaa aaaaacggaa
     3121 agaagcaata cttatcttct ttccgatgcg acctatcgcg ttaaatattt tgtatactct
     3181 tctgtatgaa gcaatcgttt agcattttcg acacgttctt ttgtaggtgg ttcgatccct
     3241 tctaatggat atttcaaacc aagtgtttcc cacttgtatt ttcccatcgt atgataagga
     3301 agaatctcta ctctgtctac atttgacaag gtttgaataa agttgtctag tcggatcaaa
     3361 tattcatcat aatcacttcg ctcaggaact agtacatggc gaatccaaac cggtttgttg
     3421 atttttgaca aatattgagc catttctaaa atattttcat tgtcccaatg cgttagtttt
     3481 ttatgctctt cattatcgat gtgtttgatg tcaaataaca gtaaatctgt atacttcatc
     3541 aattcttgaa aacgtgaaaa gaaaggttct tcatacgtaa aaggttttcc gcatgtatcc
     3601 aatgtagtat gcatcttccg ctcttttgct tttttaaata gttcgatcaa aaaatcgatc
     3661 tgcaatagag gttctccacc actaacagta ataccacctt tatctcccca gtaaggtctg
     3721 aagcgttctg ctttatctaa aagttcatct gctgtgtatt ctttccctcc gccaatattc
     3781 caagtatctg gattgtgaca aaattggcaa cgcatgcggc atccttgcat aaacacgaca
     3841 aagcgcagtc caggtccgtc tactgaaccg aaagtttcga tagaatgaac atagccgatt
     3901 gttttttctt ccatttaagt tcacatcttt tctttgattt cattttaagt acaagttagc
     3961 tgtcgaagcg aagctttctg aacaaaattt ttcgcaattg ccgcttcgtt atgtgtaaaa
     4021 tttacaggga cggaacatac aagtcctgcc cctgtaaatc gtttctttca aataagcata
     4081 ctgattattc gattttgatg cgatggctat tcaacgtcct ttcgacgcta agtcgaaaaa
     4141 cttttttcac gaacttttta cattctgtcg tgtgaagttc tcatcaagac atctaattgt
     4201 tgttctcttg tcaagtcacg gaatttaact gcataaccag atacccggat cgtcaagttc
     4261 ggatatttca ttggattttc cattgcatca cgcaataaat cattactgaa tacattgaca
     4321 ttcaaatgat aagctccttt tgagaagtat ccatctaaga cattacgcaa gttttcgatt
     4381 cttgtatctt gttcttttcc aagacctgaa ggattgattg tttgggtatt tgaaattcca
     4441 tccaatgcac agccgtaatt cattttagct gttgagttca ggctagctaa taacccactt
     4501 tgttccgcat tgtaacttgg atttgctcct ggcgcaagtg gttttccagc ttcacggcca
     4561 tcaggagtat ttcctgtatt tttaccgtaa acaacatttg aagtaatcgt caataatgaa
     4621 gttgttggtt ttgagttgcg gtaagtttta tgacgtttga tttgtcccat gaagtattcc
     4681 aagatccatg aaactaattc atctgcacgg tcatcatcat ttccgtattt agggaagtcg
     4741 ccatccactt caaaatcaac tgctattcct ttttcattac ggattgcttt tacttttgca
     4801 tatttgatcg ctgacaagct gtctgccgca tgagagatcc cagcaatacc tgtagcaaat
     4861 gtgcgttgca agtcagaatc catcaaagcc agctgagctg cttcataaga atatttatca
     4921 tgcatataat gaatgatgtt caacgtattg acatatagtt ctgctaacca atccatcata
     4981 ttcatgaagt tatgtttcac ttcttcaaag tctagtattt ctgatgtgat tggacgatat
     5041 tctggtccaa cttgggcgcc agtcacttca tcgactccgc cattgatggc atataatagt
     5101 gttttagcca agttagcacg agcgccaaag aattgcatgt cttttccgac aactgttcct
     5161 gatacacagc aagcaatcgc acaatcatca gaaccccatt gttcacgaag ctgattatca
     5221 ttttcgaatt gaatcgaaga actttcgata gcgattctgc tagcatatgt tctgaatcct
     5281 tctggaaggt gttcagaata caaaacagtc aagtttggtt ctggtgaagg tcccatattt
     5341 gttaatgtat gaagcagacg gaaatcattc tttgtaacta atgaacgacc atctaatccc
     5401 atacctgcaa tggataaagt agcccagata gggaatcctg agaataattg gttatattca
     5461 ggtgtacgcg cgaatttgac catacgtaat ttcattacta agtggtcgat caattcttgt
     5521 gcttcttttt ctgtgattac tccattatcc aaatcacgtt ggatatagat atcaaggaat
     5581 gctgagatac gtccgattga catcgcagca ccattttgtg atttgattgc tgctaaatat
     5641 ccgaagtata gccattggac agcttctttt gcatttgcag ctggtttaga aatatcatag
     5701 ccatatttcg cagccatttc tttcatttgc gctaatgcac gatattgttc agtgatttct
     5761 tcacgtaaac gaatcacttc ttcactcatt gtatgataac cagtcatatc atgatctttt
     5821 ttcttttgtt ccatcaaata gtcgattcca tacaatgcca cacgacgata gtcaccgatg
     5881 atacgtccgc gaccgtaagc gtctggaagc cctgtaatga ttttgttttt acgagcagca
     5941 cgcatttcag cagtataagc gtcaaataca ccttgattat gtgttttgcg ccagtcagtg
     6001 aaaatatgtg tcaattcatc gtttggtgta tagccattgc tttttagcgc attgttagcc
     6061 atattgattc cgccgaacgg catgaatgct tgtttcaaag gtttgtctgt ctgtagaccg
     6121 acgatttttt ccaaatcttt atccaaatag cctgcatcgt gtgaagtaat agttgatacg
     6181 atttctgtgt ccatatcata gacaccgtta cgctcgaatt ggatatcatt taacttttgc
     6241 aattcatccc ataatttttc tgttgcttct gttgggcctt ccaaaaagct gtcatcaccg
     6301 cgatattcag tgtagttatt ttgaataaag tctctagtgt ctacgccttc tttccatttg
     6361 gtacctttaa atccattcca ttgctccatt gtatggacct cctaaatttc tgttttatgt
     6421 aacagttttt acgtgattag tataacacag caccctatct ttgcaagggt tttcttgttt
     6481 tttatttaac aatttttatg ttttatttac atatatttaa atcaacgttg acataccaac
     6541 atttaagcat tgtgaacact ttcatctttt tttgcaaacg tataaggatt tattaaaaat
     6601 gaatgaaaaa gagacgccac ctgtattaat acagatggcg tctcttcatt aaaatactac
     6661 aacagttacg actcgatgaa aatctcagga gcatcgataa cttcgatgac ttctccacct
     6721 tttttctcat ccatcgcaaa tgatccattc gatgttcgat cacctatagg gaaatcagtt
     6781 gggtcaatcg ttaactgctg ccctttttga ttcatcaagg ttatttcttt tgatcttcct
     6841 tccgacatat agataatacg gtgcggattt tttttcagct cacgcatgac cgtcactccc
     6901 cgcttcgcac gtgaagtttg gctgatttct tgagcaagca tgcgtttaac agctcctcgc
     6961 tgagtaacga ttacgatcgg cgtatcacct tctgctaaca caagcagccc gttgataatc
     7021 ctatctccat ctttcaaatt catagacttc actccagctg cctttgctcc aacaacggga
     7081 acatctgtta aaggatagcg caaacccatt ccttgatgcg ttactaagaa aacatctaag
     7141 cctggctgat cttctttcag atacacagca atcagttcat cttcttccgc ctttagcttc
     7201 atacagctga gcggacggct cttataggtg cgccatggtt cgaactctgt cattcgtgtt
     7261 tgcttgatca atccattctt gctgatgaat acaaatgttt tctctgcttg aattttttga
     7321 tatgggaaaa cagctaaaat cgattcgtct acagacagat tggagattgt ttgagaaatg
     7381 tgctcaccag catctttcca tttcaaatcg ggtagttcat ggacagggcg gtagatcaca
     7441 tttgctttat ttgtgatcag aagaacatgg tccaatgtac tcaattcacc tgagtatagc
     7501 aagtagtcgc cttctcgcat cccgatttct tctggtttgg aagcactata agaacgaagg
     7561 cttgttcgct tcacatagcc ttctcgcgtc acactgacaa ttacgtcttc ttgtgccact
     7621 aagacttgcg tatcaatttt gatttcttcg atttcgtctt cgattgtcgt tagacgagcg
     7681 gaagaataat tttttttcac ttctcgcaat tcttttttca taacagagaa aagctctttg
     7741 tcatttgaaa ggattttatt caactctgtg atttgcgcaa tcaagctttc ggattccttt
     7801 tgaagctctg tgatatccgt gttcgtcaaa cggtataatt gcaatgtgac aattgcttct
     7861 gcttgttctt ctgtaaattg aaagacgtcg actaaattct ttttggcgtc ttttttgtct
     7921 ttactttcac gaatcgttgc gattacttca tccagaatcg ataaagcttt catcaatcct
     7981 tctacgatat gctgtctttt ttgagctttg gctaaatcga attgactacg ttttgttatg
     8041 acttgctttc gatgctcgat ataacttctt aaaatgtctt tcagtccaac ttgatgcgga
     8101 gtcatatggt cgatagcgac catattgaaa ttgtaattga tctgtagctc agtgtttttg
     8161 aacaaataat tcaaaattcc ttgagcattg gcgtcttttt tcaattcgac gacaatttgc
     8221 agacctgttc tatcactttc atctcgtact tcagctattc catcgatttt cttattcaaa
     8281 cggatctcat ccattttttt caccaaggtc gctttattta cttcatatgg gatttctgtg
     8341 atcacgattt gctgtttatt acccttcatt gcctctatct ttgttttcga acgaagaata
     8401 acttttcctc gtcctgtttc ataagctttt ttgatttcat cttttccttg caagatccct
     8461 cctgttggaa agtcaggtcc aggaatatat tccatcaatt tttcaaggct cgcttgtgga
     8521 tgatcgatca tatagattgt gccatcgatc acttcagcta agttatgagt cgggatttct
     8581 gttgcatatc cagcagatat acctgtcgat ccattgacga gaaggtttgg gtatcgagct
     8641 ggaaggactg ttggttcttt ttctgtatca tcaaaattcc acacgagatc gactgtttct
     8701 ttctcgatat ctgctagcat ctctccactt aacttggata atctggcttc agtataacgc
     8761 atcgcagcag gtggatcacc gtccatactc ccgttattcc cgtgcatttc gatcagtact
     8821 tcccgcaact tccagtcttg gctcatgcgg accatcgctt cataaatact gatgtcacca
     8881 tgaggatgat aattccccat gatatttccg acagattttg ctgatttacg gaatcctttg
     8941 tcatacgtat tgccatcttt gttcatggca aataatatcc ggcgttgcac tggcttcaat
     9001 ccatcacgga tatcaggcag tgcacgttct tggataatat atttggaata acgtccgaag
     9061 cggtcaccca tcacttcttc taaagttaat tcttgtactt cttgtcgatt ttccatcagg
     9121 tgacctccta ttctacttca aataactgat tgttttcatt tttatctgct cgttcttcat
     9181 ctaataaatc attagaaact gaaggagaga tttcagtatc ctctttttta tctaagatac
     9241 tgccatcttc ttcgagcgta aattgcacgt gattttcgat ccattttctt cgaggttcta
     9301 ctttgtcacc catcagtgtg gttactcgac gttcagcttg cgcagcatca tcgatacgga
     9361 cgcggatcaa cgtacgggaa gtcggatcca tcgttgtttc ccacaattgt tccgcattca
     9421 tctctccaag tcctttatat cgctgcagca tatatccttt gccgactttt ttaatcattg
     9481 ctgccaattc gtcatctgtc cacgcatatt caatgacttg cttctttcct tgtcctttgg
     9541 atactttata taaaggcggt aacgcgatat atactttccc agcttcaatc agcggtttca
     9601 tataacgata aaagaaagta agcagtaata cttgaatatg ggcgccgtct gtatccgcat
     9661 cggtcatgat gattactttg tcgtaattac agtcttcgat agaaaactca ggaccgactc
     9721 ctgcaccaat cgtatagatc atcgtattga tttcttcatt tttcaagata tcttgcattt
     9781 tagctttttc ggtattgatg acttttcctc gtaaaggtag aattgcttgg aactttctgt
     9841 ctcttccttg tttagcagat ccgccagcag aatccccttc gactaaatac aattcatttt
     9901 tcttagggtt tcgggattgt gcgggtgtca attttcctga aagaagagat tcgccttttt
     9961 tacgcttctt cccatttcgg ctttcttctc tggctttacg tgctgcttct ctcgcttctc
    10021 tggctttgat tgctttacgg accaacatct ggctcatttc gctattttcc tgcaaataaa
    10081 agcccatttg ttcactgatg acgttatcta ctacggtacg agcaactggc gtaccaagtt
    10141 tttcttttgt ttgcccttca aattgcagaa gattttcagg tacacggata gaaagtacag
    10201 cagctaaccc ttctctgaaa tcactacctt ccagattctt gtcccgttct ttcagcaaac
    10261 caacttttct cgcatattca ttataagatt tcgtcatcgc cgctttcata ccagcttcat
    10321 gggttccccc atcttttgtc cgaacgttat tgacaaaaga taaaacattt tctgaataac
    10381 cgtcattgta ttgataagcg acctctactt cgataccttc tttttctcct gaaaaataaa
    10441 caactggtgt caatgtatct ttttcttcat tcagataatc aacaaattct ttgattcctt
    10501 cttcgtaatg aaaaatttcc ttgactggtt cttctccacg aagatcagaa agagagattt
    10561 taacgccttt tagaagaaag gcagattctc ttaaccgttc ggctaaaatt tcatatgaaa
    10621 atttcgttgt actgaatatt gtatcatctg gtaaaaaatg gacagtcgtc ccgtttgctt
    10681 tttttgtttt tcctatcttt ttcaaagtac cgtcaggttt accgccgttt ttaaatttct
    10741 gctgatactc tacgccatct ctaacgatcg ttacaattag ccatttcgat aaggcattta
    10801 ccacactggc tcctacaccg tgcagtccac cagaagtttt gtaccctcct tgaccaaact
    10861 ttccgccagc atgcagcaca gtaaaaatca cttcaactgt tggtattccg gaagcatgca
    10921 ttcctacagg catcccacgt ccgctatctg caacagttat actattgtct tcatggatgg
    10981 tcacatcaat ctcactgcca taacctgata atgcttcgtc aactgcatta tcgacgattt
    11041 catagactaa atgatgcaag cctcggctgt cagtggatcc aatgtacatc cctggtcttt
    11101 ttctaactgc ctctaatcct tccaatactt ggatggacga gtcattgtat tcgttgttta
    11161 ctttttttgc caaggcaaac gctccttcaa taatactttg cggcaaaacc gcaccacgat
    11221 tatcatacta gaaaaggtcg tgaaaaaaaa gagggtagac gagaaatcaa ttttaattat
    11281 ctcctaaccg ctttttcgac attcccctct atcgatcatc tcttctttgt ggtcattttt
    11341 atttttggtt ttctttcaac ttaccaagtt caatcttaat acaacgatcc attacgatat
    11401 ctgtacgtcc tgcttttctt agcatctcag ctgcttcttt gttttcaatc cctaattgtg
    11461 cccagaacac tttagcatca gttttcaaaa aatcacctgc tatttcaggt aaaaattcac
    11521 tccgtctaaa tacatctacg atatcgattg gatcatcgat ttctgtcaaa ttcgcataga
    11581 ctttctcgcc tagaatttct tcaccagcca ataatggatt tactggtatg attttatatc
    11641 cattgtcttg caatacctgt gcaaccatgt aacttgttcg ttctggttta ttgctcaagc
    11701 ctacgacggc aatatttttt gcttcttcca aatagttgaa aatcgtttct tgtgttggat
    11761 tttttacaga catttctgcc accccttgct tttcttttta ttccatctat tatacttgta
    11821 tctttcattt tttcaattgt tcctgttacc tattcatgct aaaataaagg atagttttca
    11881 acttgaaaga gggtaaaaaa tgaaattgat cttattactt attttagcat atcttttagg
    11941 ttctattcct tccggtgtct ggattggcaa attgtttttc aaaaaagata tccgacaaca
    12001 cgggagcggt aatactggaa cgaccaatac atttcgtgta ttaggcaaaa cagccgggat
    12061 tgttgtcctg cttatggata ttttaaaagg tacattggct acttgtttgc cgatgatctt
    12121 ccacctttct gggattaacc ctctttggtt tggggtatgc gccattcttg gccatacatt
    12181 tccgattttt gctgggttca aaggaggaaa agctgttgcg acaagtgcag gaatgctttt
    12241 aggatttaat cctgtatttt ttgtttattc ttgtatcatc tttatcgtta gtttattttg
    12301 tacaagtatg gtgagtttga gcagtatgat cagtgcagta ttgatcactt tatctacgat
    12361 cgtccttcct tatgtagctc ctgtgatttt ggctaagccc aattggttac tgacgatcat
    12421 tgccttttta gtttcttcct ttatctttta tcggcataaa gacaatatca agcgtatcag
    12481 aaaccatact gaaagtcgaa tcccgtttgg tttgaatagc agcaagaaaa aatcctgata
    12541 ctttttcatt ccaaagagat aaaacgggag tgtgacaaat cgatgtcaca ctcccttatt
    12601 acttacagga tagctttttt acacaccttg gtaaacggtg ttccctgcta acttctcggt
    12661 cacaacctct tacttcactg tgatggaata ttttgtcttg aagatttcat gggcacctag
    12721 ttgattgatc cctacttttt ccatcaagtt tccactactg ttcaccgtgt cagcgattcc
    12781 gcaccacggt tcgatacaaa cgaaaggcgc ttcttttgga taaggagacc agatccctac
    12841 ataaggcata tctttatagc ttaaagttac gctatgggaa gatttttcac ttcgaatggc
    12901 gtacgcattt agaccccttg tttcaaaaac caaggcatct tgctcaaaaa gttcacgatt
    12961 caaagccaga ttcgtatttg tctgacctaa cgtcttttga tccatatcga tgaatgggcc
    13021 tttcaatggt aggcgtaatc ttgatttcaa tggagaaaat gccagataga aatcttcaaa
    13081 tgacaatcct tcttcaagag gtacattgaa agcaggatgt ccaccgatag aaaagaacat
    13141 ttctttttca ccggtatttg ccacttgata atgaaccgtg atgccgtctc ctcctaattc
    13201 atagctgatc actaattcaa aatcaaatgg atacttttct aatgtctccg gcgtactcct
    13261 taagatgaaa ctagccattt cttgtccatg ttcaatgact tcaaaatcca tatcccgagc
    13321 aaatccgtgt tgccccattt gatatgtttt cccttgataa acatactgat catcttttaa
    13381 acgtccaaca aaagggaata aaaccggtgc atgtctgccc caatacgctg gatcaccttg
    13441 ccagatatac tctaaaccat gttcttttga tttcagactg atcaattccg caccgtgttc
    13501 agcgatttcg gcgatcagct ggtcattttg aataataaca gtcattgcca ttcctccaac
    13561 ttcgacttac tttttaaaat gggaaggctg gaacaaacat ttctatctgc cccagtcccc
    13621 ttacttctaa taactgtgtt ttgctaactc gtctttaaaa cgggaattca atacttttaa
    13681 ataagttccc ttcatgccta acgaacgaga ttcaatgatt ccagctgatt ccaattttcg
    13741 taaagcattg acgatcacag aacgagtgat gccgatacta tcggcaatat tagaagcagt
    13801 cagacgacct tcatctccat ccaatgcttc aaagatagct tgtacagcct tcaattcgct
    13861 ataagataat gtgttgattg ccatttggac agccgttgca ctccgtacat ctgcttcgat
    13921 gcttcgagac ttctgataaa ggatctgcat accgacaacc gtagctccat attctgctaa
    13981 gactagatcg tcatctgcaa acgattgttc catacgagat aagataatcg tacccaaacg
    14041 ttcgccagct ccaaagatcg gaacgacagt cgtcagacca aatggatatt tctctcttga
    14101 ctctaccgga aatgcagtaa tatcactagt aattgggata ttagcttcag ttttcatcaa
    14161 ctgtgccact gtatcagtat aagattcagg aaattgtttc tcaacaaaca ttgttttgat
    14221 tcgttcattg ttcacatcgt gacgttcatt ataacctagt aatactccgt cacttcctat
    14281 gatataggtg ttgctgtcaa gaataccacc tagtgtgaca gccattttat cgtaaggcaa
    14341 atctgcttgc atgtcaaaag tattcttttg ctgcaaaagt gtgtttatct tgcgggtttt
    14401 cgttagcaag tctgtcattg tcgtcctccg attttttata aaatatagcg acttaaatct
    14461 tcatttgccg caattgagcc aagtttgtca tccacatatg cttctgtgat cgttatttct
    14521 cccatttgca tatctggtgc ttcaaataat agatcttcta gtagtttttc caaaatggta
    14581 tgcagtctgc gtgcgccgat attatctgtg tcacggttga catcgaaagc gatttgagcg
    14641 atccgttcaa ttgcttcttt tgtaaacgta actgacacat tttctgtacc gatcaacgcg
    14701 acgtattgtt ttactaaagc attatttggt tcggtcagaa tcttcacaaa atcttcagct
    14761 gtcaaatcat ctaattctac ccgtatcggg aagcgtcctt gaagttctgg aattagatca
    14821 cttggtttag acaaatggaa agctcctgaa gcaatgaata ggacgtgatc tgtttggatg
    14881 atcccgtatt ttgtgtttac ttgtgaacct tcgacaattg gcaatatgtc tctttggacg
    14941 ccttctctgg atacttctcc agattgctgt gatttagaag tgatcttatc gatttcatcg
    15001 ataaaaataa ttccactttc ttccgctaat ttgattgcct cactatggat atcagcatcc
    15061 ttgactaatt ttgctgattc ttccttgatc aataactcta aagcttcttt gactgttacc
    15121 gtccgttcca cttttttctt cggtgtcaaa gcacctagag tttcatttag gtcgatcccc
    15181 atttgctcca atccattgtt catcattggt gcaggtttct tttggtcttc aacttcaatg
    15241 gttacttcac gactatccag taaacccttt tgtagttgtt ccaagactgt acggcgattg
    15301 attcgaatat cttctgtcac ttcttcttgc ggttcagcgg tattttgctg actagcatta
    15361 aaaagattca tcatttgttc aaattgatta gtggattgtt tctgttcttt tttgatacca
    15421 ggtaccaaga tttttaccaa acgtttttct gcattttttt tagcacttgt gtatacacgg
    15481 ctgtactgct ctttttctac gatttggatc gcattttcta ctaaatcccg aaccattgat
    15541 tctacatctc ttccgacata accgacttca gtaaattttg ttgcttcgac tttgacaaat
    15601 ggtgcattga cgatttttgc caaacgtcgt gcgatttctg ttttaccgac ccctgtaggt
    15661 ccgatcatca gcatattttt tggtgtaata tcttgttgca ttttttcatc taactgcaaa
    15721 cggcgatagc ggttacgcaa agcaacggca acagattttt ttgctgcgtg ctgtcctacg
    15781 atatattcat ctaattcact tacgatttgt cttggtgttt gttttaattc atacatatct
    15841 actcacctct tacagttctt ctacgataat attatgatta gtaaagatac agatatctgc
    15901 ggcaatattt aacgcatttt ctgcaatttc tttcgcactc atgtcgtttt gagcatagtt
    15961 tttcattgct cgggctgcag ccaacgcaaa attccctcct gatccgatag ctaagatacc
    16021 atcatctggt gtgataactt ctcctgtacc tgaaacaaga agcatttctt tatcattcat
    16081 tacgatcagc atcgcttcaa gtttttgcat agattgctgt gtccgccatt cttgagctaa
    16141 ttcgacagct gcgcgttgta gattcccgtt atattcgttt agtttgcctt caaatttttc
    16201 ttctaaagta aacgcatctg caacacttcc ggcgaaacct acaacaactt ctccattgta
    16261 gatacgtcgt actttctttg cagtgccttt catgacaaca gattcaccca ttgttacttg
    16321 gccatctcca gccatagcaa atttcccgtc tttttcgacg gcacagatag tggttgaatg
    16381 aaatggtgat tcgaccatgt ttgtttcctc ctaaatttgt tcttttcctt atttttacgc
    16441 tcttggatgg aaagaacgat agtttttttg taaactttct tttgttacat gggcatagat
    16501 ctgggtagtc gataaattgg catgtcctag caattcttgg actgtccgca aatctgcccc
    16561 attatttaat aaatgagtag caaatgtatg gcgcagcatg tgaggatgga tatcactatc
    16621 caaactgctt tttttgatca tttgattcaa gacatattca attccagtag gcgtgattgg
    16681 ttttccatga tgattcacaa aaacgatatc atgctcttcg tgatattttg tcatcaatac
    16741 ctggcggcct tccctgaaat attcttgcag agcatcttga gcaaaagaac ctagaggagc
    16801 atatctctct ttgttccctt taccatggat caacatgacc tctgaatcaa aatcaatgtc
    16861 tgataccgac aaactgctac actcacttaa acggatgcct gacccgtaaa ggacttccag
    16921 taaagcgcgg ttcctcaaat ccaaaggact atctcctgct acgctgtcaa aaagtgcttg
    16981 catttcattt tcataaaaaa aacgtggcag tctcaaattt ttctttttga gatgaacgta
    17041 agaaaatgga ttttctttta tcacttcttc tttgagaaga tactgataaa acgaacgtag
    17101 gctggctatc ttgcgactga tagaatttct gctatagcta ggacgatgat cagctaatgc
    17161 actcaaatag acacgaaccg taaaatattc cgccttcagc agatcagcgt ctccagattc
    17221 ctccaaaaaa tgtttgaaat ggagaatgtc ttcttcatat gcttctttcg ttttttctga
    17281 atatcctctt tcgacaatca aataacgcaa aaatttttgt tcccacgtgg cttctgtcat
    17341 tttctcacct tccttttgtc ccagagaata atttagcaca gcgtttataa aaagacaaat
    17401 aaattgtcag aatttaacca atatttctaa gtttgttcac aattggcgca attcctcaca
    17461 ttttgtttga gaaattctta agaaaaacgc aagaaaaaga gcgagactat ccgtctcacc
    17521 ctcttcatgg agtttcctta ctcatttggg attgcagttt tttctaattc tgtttttatt
    17581 gcctcattag cttttaaagc tcgttgtgca attgcttcat atcttgcttt tttgtctcga
    17641 atgcgctctg gcagttcagg aaacagacca tagttggcat tcatcggttg gaaatgtttt
    17701 ccttccgcat gagtaatgta gtaagccata cttccaatag ctgtttcttg tggaaactcg
    17761 actggttcca tttcttttgc taagcgtgcg gcgttgattc cagcaagtag tccgctcgct
    17821 gcactttcaa catagccttc tacaccagtc atctgacctg caaagaacag atcttcgcgt
    17881 ttcgttgatt ggtaagtcgg tttcagcaat tctggcgaat tcataaagct gtttcggtgc
    17941 atcacaccat agcgaacgaa ttcagcattt tctaacccag gaatcatccg gaaaacacgt
    18001 ttttgctcgc cccatttcaa gtgagtctga aagcccacga tgttgtataa agaagctgca
    18061 gcattgtctt gacgcagctg gataacagca taaggacgtt tgccagtttt aggatcttcc
    18121 aatccaactg gtttcatagg tccgaaaagc atcgttttga atcctcgctg tgccattact
    18181 tcaattggca tacatccttc aaaatatttt tctttctcaa aagatttaag ttctgccact
    18241 tcggcattga ttagtgcctc atggaaagct ttgaattcct cttctgtcat cggacagttc
    18301 aagtaagctg cttctccttt gtcatagcgg gacttcaaat atactttatc catatcgatc
    18361 gtagctttgt ctacgattgg tgcagctgca tcatagaaat aaaaaccatc tgaaccattg
    18421 aatgctttga tgctttctgc taatggatga gaagtcaaag gtcctgttgc tactacggtg
    18481 attccttccg gtatttcagt aatttcttcg tttatcactg ttactaatgg atgctctttg
    18541 acttttcggg taattgtttc agaaaaagaa tcgcggtcaa cagctaaggc accaccagct
    18601 ggcactgctg tttcatctgc cgaagtgatt actaccgaat tcaatcggcg catttcttct
    18661 ttcaataacc ctactgcatt agccaaactg tttcctctta atgaattgga acatactagt
    18721 tcagcaaaat tacttgtgtg atgcgcctca gtcgatttga ctggacgcat ttcatataaa
    18781 cggacaggga ctcctgcttg tgcgatctgc caagcagctt cacttccggc aagccctgct
    18841 ccaacaacat ttaccatttt caatgaaaga acctcttttc tattattttt ggatgtcttc
    18901 ttcgtaatct ccatttacac agactacttg tttaccgcct ttgactttct tctcgaccag
    18961 atatccgtca cattttggac aatttctgcc cactggttta tcccaagaag tgaattcaca
    19021 ttctgggaaa cgggaacaac catagaatag tctgtttttc ttagattttc gttcaatgac
    19081 ttgtccttca tgacatactg ggcaagtcac accgatttct tttacgattg gtttcgtatt
    19141 ccgacaatca ggaaaattac tgcaagcata aaacttccca tatttcccta attttatcac
    19201 catcggatgt ccacagacat cacaatcgaa tcctgctggt tcatctttga tttgaatttt
    19261 ttcgattttt tcttccgcat tcgtcagttc tttttcaaac ggtttataga aacgatcaac
    19321 cactttgacc cacgcttccg tgccttcttc aattttatcc aaatctcctt ccatttctgc
    19381 tgtgaaatgg atatcgacga tttgagggaa gaattcacag attaatgaat tgacaatttc
    19441 tcctagttca gtcggctcaa atcttttttg tgtcaatttt acatagtagc gtctttggat
    19501 cgtatcaagt gttggtgcat aagtggaagg tcgccctacc ccgttttctt ctagtgcacg
    19561 aatcaacgta gcctcactaa aacgtgctgg tggttgagta aaatgttgtt ttggttcaat
    19621 atcaacagat tggactacat cgccttcttc aagttctgga agaatatttt ctttctcttc
    19681 tttgccgtca tctctacctt ccacatacac ttgcatgaat cctttgaatt tgatttttga
    19741 accgttcgca atgaagatga caccattttg ttctaatgtc actttcattg tatctaaaat
    19801 agctggtgtc atctgactag ccacaaaacg tgaccagatc aatgtataaa gtttcaattg
    19861 atctttatct aaatattgtt tgagttcatc tggtgtacga agcacactcg ttggacgcac
    19921 agcttcatgg gcatcttgtg cgccttgagc atttttgatc tttcggtgat catgtctaga
    19981 aaattctttt ccatacgtat cttcaataaa tgctgcggcc tctgcttttg ctgtatcggc
    20041 aatccgggta gaatcagtac gcatgtatgt aatcaagccg actgttcctt gtttacccaa
    20101 agtgattcct tcatataatt gctgtgctac catcatggtt tttcttgttc gaaagtttaa
    20161 cttacgggca gcttcttgtt gcaggctact tgtagtgaaa ggattcgcag ggtttctttt
    20221 gcgctctttc ttttctactt ttttaacttc gaagtctgtt ccatcaattc tagcagtgac
    20281 ttcttttaca ctttgtgcat ctggtaattt ctttttctta ccgtcgattc cccagaaatt
    20341 tgctttgaat ttcttgcgag cttttttaaa attaccgtcg atgctccaat attcttctgg
    20401 aacaaattct cggatctctt tttcacggtc aataataatt tttaaagcga cagattggac
    20461 acgtcctgca cttaaccctt tcttgacctt acgccagaga ataggactga tcgaataacc
    20521 aacgattctg tctaaggtac gacgagcttg ctgtgcatct actaaatcta catcgatcgt
    20581 tcttggttcc ttaaaagctg ctttgactgc gtcttttgtg atttcattga agaccacccg
    20641 atttttttct ttcagatcca acccaagtag gtacgctaaa tgccaagcaa tcgcttctcc
    20701 ttctctatcc gggtcacttg ccaagtaaac tttttgagct ttttttgctg cggctttcag
    20761 gcttttgatg acatcgcctt ttccgcgtat agaaatataa tggggttcat aattgttttc
    20821 aaaatcgata cccattttac ttttcggtaa atctcttata tggccaacac tggccattac
    20881 tttatagttt cgtccaagat atttctcaat tgtcttggct tttgcaggtg attctacaat
    20941 cactaaatat ttatacgcca ttactgaatg gactcctttc cattgtcgtg ttcctactat
    21001 taaaagagga ttcatacaaa tcgtagctaa tcatatccat tcagaaaacg tttgtcaaga
    21061 aaagcaaatg aaaagtatgg tgttattctt taaattctgc caagatatct tcacccgaaa
    21121 ccgtgcaaac tgcaccgtct tggatcaaat gatgacatcc ggtagaatgt tctgttaaaa
    21181 tatttcctgg aacagaaaaa actgttcttc caagatccaa tgcttgctgt gctgttatca
    21241 gagagccgct ttttttcttg gcttcaaaga cacatgttcc tttagacaaa gctgcgataa
    21301 tccgatttct cattggaaaa caatgctttt tcgggccgga ttgaggagga tattcactca
    21361 aaagaagatg atcatttgca atcacctctt gcaagtcacg attttcagca ggatagactt
    21421 gctctatccc tgttccaatg actgcgattg tccgtccacc atgagagatt gctgcttggt
    21481 gaccgtatgt atctatccct ttcgcgcagc cactgacgat cacatatcca gcttctatca
    21541 ctggttttag taaataataa actgtttttt tggctaaagc agaagtgtgt cttccaccaa
    21601 caacgcttaa catgttacaa gaaagtaaag atagatcacc attataaaac agtacaattg
    21661 gcggataggt caagtgaagc agctgagggg gatatttggg atcaaagcaa gtgataaacg
    21721 actgtttcga ttggattttg ccccagttag cgtgaatttc ctgccaactt ttgcgaaaca
    21781 gctcccggtg tttggttatt ttacctatct taatgatgtc ttcaaatccg atttctttat
    21841 aaggatactg catcatccag ttagcaagtg cccattttcc ttgactactg attccttttg
    21901 tataagcaag tttcaataaa ttttcttcta tttgatacat cataaaaccg cctttcgtct
    21961 ctctttctaa taagatacgt aaaagcggct gttttttatt gtaaatattt ttttacaggt
    22021 gcaaaacttt tacgatggat tggcgtaatt ccttgtcgtt caagtccttc taagtgttcc
    22081 tttgttccat aacctgcatt atgcgagaaa ccatatccag gataaagcac gtcatattct
    22141 ttcatcaatt ggtctcgata tactttagca atgatacttg cagcagcaat cgacacagat
    22201 cgtgcatccc ctttgattat tttttcctgc tgaataggca aatccaaggt catcgcatcg
    22261 atcaacaaat agcttggtac aatggacaat ttttctactg cttggatcat cgctaattta
    22321 cttgcttcat aaatattcac atcatcgatt gtctgctgat caacgatccc gatcccgatt
    22381 gccagggctt tttcttgaac ttgttgataa agaagttctc gctttttctc tgataattgt
    22441 ttggagtcat tcagccctaa tatgatctca tttttcggca agataactgc agctgccacg
    22501 acaggtccag ctaatggtcc tcttcctact tcatcaatgc cggcaatcca ttggtgacct
    22561 tgtgcgtacc cttgcttttc aaagcaaaac atttcttcat atttttcctt aattaatgct
    22621 tcctttttca atcgtcgttc aaaagaagcc agtgcttgct gcacgccttt gcgtggatca
    22681 tttcttaatg attctagtgt aggatcatct attctctgta tgcttttcaa tcgttctttg
    22741 atactttgga tggattcagt catcactttt ttgctccatt tcttcgcaac gatccagtgt
    22801 gtatcgtcca agttttcctt gacgaatatc atgcacgact aattcgctgc cacgatcata
    22861 gtcttcttta tacccacgtt ttgctgtaat atccatcagt aattccggtg ccaatttttc
    22921 ttcttgctcg attgataaac cataacgatc gctgattctt cctgaataat atcgggcaaa
    22981 aaagtctaat ccgtaaatcg ctaaatcatc taaatggaca agctggtctt tgatcgcgcc
    23041 tgtcagcgct agttttttgc cgatttcttc atcttcaaat ttgggccata aaatccctgg
    23101 tgtgtctaac aattccaact cattacctaa acgaagccac tgctgacctt ttgttacgcc
    23161 aggcttattt cctgtctgtg caattttctt gcctactagt cgattcatca acgtagattt
    23221 cccaacattt ggaataccta agaccattgc ccgaatagcg cgaggcttga tcccccgagc
    23281 agcatctctt gcccgttttt ctttcaatgc ttctttagcc gcaggtacta gacctttaat
    23341 tcctttactt tcttgtgccg tgatagctaa tgcaagatgg ccttgtttac gaaaatattc
    23401 ttgccacagc tttgtctgtt gtggatctgc cagatccgct ttattcaata aaacaaggcg
    23461 aggtttttgc tgaatgatct gatctagcat tggattacga gaagataaag gtaatcgtgc
    23521 gtctaccaat tcaaatacga tgtccacata tttcattttt tcagaaactt ctcgtcgggc
    23581 ttttgccata tgtcccggaa accattggat ggtcattgtt ctctccattc ctttcatacc
    23641 ggaatattcc ccggctttac ttgttttttt ctttttcaat ataaattcac ttttttatta
    23701 taaagaaatt ccatctatta gttaagtgaa agtgaaccag tcctcattct atcacatctt
    23761 cacttttttg atgatgcaaa tttttatttt ggtttctata tatgaatcta aatttttgac
    23821 agcgtttcct aaataagaga tgatagacga acaaaacaga aaggagacga aacaaatgaa
    23881 tagtatggtt tttgttaatt ttcctgttgc tgatgtcaat cgttccgttg ctttttatga
    23941 aaagctgggt tttaaaaaaa atgaagaatt ttctactgaa gaa
//
LOCUS       DANKHL010000017        23845 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000017 DANKHL010000000
VERSION     DANKHL010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.019"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(501..1055)
                     /locus_tag="PW101_000494"
     CDS             complement(501..1055)
                     /locus_tag="PW101_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDL2512925.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(1181..3110)
                     /locus_tag="PW101_000495"
                     /pseudo
     CDS             complement(1181..3110)
                     /locus_tag="PW101_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(3416..4132)
                     /locus_tag="PW101_000496"
     CDS             complement(3416..4132)
                     /locus_tag="PW101_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL2512926.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(4133..5956)
                     /gene="glpO"
                     /locus_tag="PW101_000497"
     CDS             complement(4133..5956)
                     /gene="glpO"
                     /locus_tag="PW101_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HDL2512927.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5970..7466)
                     /gene="glpK"
                     /locus_tag="PW101_000498"
     CDS             complement(5970..7466)
                     /gene="glpK"
                     /locus_tag="PW101_000498"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDL2512928.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7602..9062)
                     /locus_tag="PW101_000499"
     CDS             complement(7602..9062)
                     /locus_tag="PW101_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2512929.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(9052..10347)
                     /locus_tag="PW101_000500"
     CDS             complement(9052..10347)
                     /locus_tag="PW101_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2512930.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10679..11356)
                     /locus_tag="PW101_000501"
     CDS             complement(10679..11356)
                     /locus_tag="PW101_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HDL2512931.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(11386..12477)
                     /locus_tag="PW101_000502"
     CDS             complement(11386..12477)
                     /locus_tag="PW101_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDL2512932.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12578..13012)
                     /locus_tag="PW101_000503"
     CDS             complement(12578..13012)
                     /locus_tag="PW101_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2512933.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13596..15053)
                     /gene="ascB"
                     /locus_tag="PW101_000504"
     CDS             complement(13596..15053)
                     /gene="ascB"
                     /locus_tag="PW101_000504"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2512934.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(15050..16945)
                     /locus_tag="PW101_000505"
     CDS             complement(15050..16945)
                     /locus_tag="PW101_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2512935.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(17003..18778)
                     /locus_tag="PW101_000506"
     CDS             complement(17003..18778)
                     /locus_tag="PW101_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL2512936.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18828..20324)
                     /locus_tag="PW101_000507"
     CDS             complement(18828..20324)
                     /locus_tag="PW101_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2512937.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20518..22140)
                     /locus_tag="PW101_000508"
     CDS             complement(20518..22140)
                     /locus_tag="PW101_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL2512938.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(22468..23781)
                     /locus_tag="PW101_000509"
     CDS             complement(22468..23781)
                     /locus_tag="PW101_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL2512939.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
BASE COUNT         6850 a         5203 c         3940 g         7852 t
ORIGIN      
        1 tttgacctaa gtattagaga aaaaaacggg aaaatagctc ctcatatttt gccgtttttt
       61 gagctaatac cgaaggtcag ctgattgaac accgtttaag gaagagattg tgaaaaagtc
      121 gcttagtttc gagtattaag aaaaattttg tgaaaatgga tttctacatt tttgcataaa
      181 tttggcttaa taccgagaaa ctggcttttg aataccgttt aaggaagagg ttgtaaaatc
      241 agcggtttga cctgagtatt agagaaacaa acggaaaaat agctgctcat attttgccgt
      301 tttttgagct aataccgaag gtcagctgat tgaacaccgt ttaccaagag gttgtgagcc
      361 taccatttaa ctccgaatca caagaaataa tttcaaaaac aacttctcct gtttttggtt
      421 cggttggctt attatcgaga agtagctact gggagtctat ttatcaaaaa aaggaatgcg
      481 acataggtca cacccctttt ttattttctt tgaaacatct ttcggtactc gacagcatac
      541 aagacaaaag attgtaaaag accgtaagtg atgacggcaa aaatgactct cacccacagc
      601 gttggtaatt ctgggaaaag cagcaaaagg atcatgccaa catagatgac agcagtcgct
      661 actttgccat accacttcgc tccacttaat ttgatatttt tagatagccc gattaatcca
      721 gctacaaaca taaagccttc ttttatcaag aatagaccaa acaacagcca aagccacggt
      781 cgataccatg ccatagacaa aatcagaaaa agttgtgtca atttatctgc aaacggatca
      841 agcaccttcc ctaagtctgt tacctgatta tatctccgag caatgacccc gtctagataa
      901 tcagacaaac ctgagacaac aatgaccgct cctgctatat aaaatgactg tctcagcaca
      961 aaataaagat aaatcggcag caaaaaaata cggaatatcg ataaaagatt cggaatcgtt
     1021 tgtatttctt tacgccagtc tgtgcttaat ttcattttat cgccctacct cctcagcact
     1081 cttttctttt tattttagct tacacgggga ataaaaccaa tgatttcact gtttttattt
     1141 ttctttacag acactgatat tatgagctaa tttgagataa tcacttgctg ttttcatcgt
     1201 ttactcttct ttcttcctgt cgtttgcgca tttcttttac tagtacttta tgagcttttt
     1261 ttgatcctcg ctctaggtca cgctgccgtt tacgtgtttt ataatcgata aacagactgt
     1321 ctaaatcgta acctgtcggg tacagatctt ctgctgaacg caccagacga acacgctgac
     1381 gaggcacgtg gattattttt ttgtctaaat atacatcaat cgtatcttca cctatatctt
     1441 cataaactaa cgcttctttt tggtattcat tgacaaatat ccgatcacct tttgcaaaga
     1501 gctgacgttc ttttttattc tccttcctat ttgtaactgc tgtcttagga agaaattctt
     1561 ttttctgtgt ccgataggct ttttccgcca agtaacgctc tgcttgatgg atcaatttca
     1621 tcgacatatc cattttatga gcaatccaca aagcttggct ttctcctgtt tcttccaacc
     1681 cgcaactgat atttaggacg aagtgcttcc cgatcgaaag ccatagcagc cggcacaaaa
     1741 tcttcatgat ctccggcgaa tttcttgatt tctccataat gggtcgtggc aatgatcaat
     1801 gccccttgct catacataga ttccatgatt gcgatcgcta gtgcagcccc ttcatttggc
     1861 tctgttccac tgccaatctc atctaaaagt accaacgtat tcctttttat ctttttcaaa
     1921 atagcagcga tgttttgcat atgaccagaa aatgtactca acgcattttc taaattttgg
     1981 tggtcaccga catcgacgaa aatctcatca aagacagcaa gctctgtccc ttcttttgcc
     2041 ggaacttgca taccgaacat cgccatcaaa gtcaataaac cgactgtctt caacacgact
     2101 gttttgcctc cggcatttgc tccggtgata atcaagcctc gatagtcttt accgatttca
     2161 acatccaatg gtaccgcatg ctcaggtaaa aatggatgtc tgccttgctt gataatgata
     2221 tgttccgatt tattgattct aggtgtgctt ccattgattt ctcggctaaa ttttgcccga
     2281 gcaaagatga tatctaacac cgtgatcgtt tcgatcaata aatcgatcac ttcttcgttt
     2341 tctgcgatag caccagtcaa tgccgccagg acttgatatt cttcactgat ttcttcagct
     2401 tttaacaact gatagtgttc attcaattta gaaatcacag ttggttcaat aaaaaccgtc
     2461 gttcttttat ttgattcatc gataatcgtt ccatctattt tatttttata gctggctttg
     2521 ataggaatcg tatagtattc gcctttttga acaatcattg cttcttggat catttctttg
     2581 tttttcggat gacgcaagaa tttcagtaat tttgactgga tttctttttc gattagttgt
     2641 aattgtttgc gtactttacg caaattacgt gacgcgtcgt cactgacttt attatttttt
     2701 attttctggt caatcaactc ttctacattt atcaaatcag gaagatgttt gctataagca
     2761 aacaacaagg gtgtctggta ttgattctta tcaaaaaact ttgtgatcat ccgactgctt
     2821 ctaagaaaat ccgcatattc gatcaaatcg gttggctgca gaatcaaccc ttttttgact
     2881 tgttctgtca gtgcatcgat cctcggcaac cccataaatg gaacatgctg actgctttct
     2941 aagatcaagc gtgcttctct tgtttcttct tgacgatcca agacagtttg taggtttgtc
     3001 gagaccgtca tatcgctgat tcgttttttg ctatagtttc caattgctct tgcttggatt
     3061 tcctttttga tccgatcaaa ttgtaattga ctaatcattt cgttgtccat attctctcct
     3121 tttcttgaag agatacctgt cccaaaataa ataagggcct agagataaat aaaaggatag
     3181 aacacagctt aaaaaagtca tgtcctatcc ttattagaat acaaagatat tgttttcctg
     3241 acaggtaccg tttcataagc acaccaaata aactttgttt attggctatt cgacttactg
     3301 attagatacc agctacactc acaaaacata ccccattctt ctaggtctta tttattttcc
     3361 acttaactat agcaagacac attttggttg tcaaacttta gtatgtttta tagatttaca
     3421 ccattaatga atagacacct accgcgataa ttgctccgat cattggacct gcgattggta
     3481 cccatgaata tgcccaatct gaagttcctt tattagctat tggcaatacc gcatgtgcta
     3541 atcgaggacc aaagtcccga gcagggttga ttgcatatcc tgttggccct cctaatgaaa
     3601 gaccgatggc taagatcaat ccacctacag cgaatacgtt cgttccaggt gcaaattcgt
     3661 tttgtccaaa agctagcaac ccaagaacta atacaaatgt accaattaat tctgtgatca
     3721 catttgctgg ataattccgt atagcaggac ctgttgcaaa cgttcctaaa atcgcgcttt
     3781 catctttcgt tgcattccaa tgtggcaaat aagaaagcca aacaagaatg gcccctgcaa
     3841 aagctcctaa aacttgcgca atgatgaaag gaacgacaag attccaacta attgcccctg
     3901 tcatcgccat agctagagag actgcaggat tcaaatgagc tgggcccatg aatccagaaa
     3961 tatagacagc cattgttaca gcgagtcccc aaccaaatgc gatgacaatc cagccagaag
     4021 cctgtgcttt acttttgttt aaattcacag ccgcacacac accatctcca agcaaaacta
     4081 ggatcagtgt cccaataaac tcccccatga tttgagtcat atcactgttc atttatttct
     4141 ccccctttag ttcccgcaga tctgattctg caatcaatgc ttctaaacgt ttagtctgtt
     4201 gcgccttctc ctcttctgtc cattcaaaat atccagcaat tgcatcgatc acaggttgct
     4261 tgatctcatc aagctgatct cgttcaaaca acagatgatt tgtacgacga atgagataat
     4321 cgcctggggc caaaaccatt tcttcttcta aaccataacg taaccgagct gactcagcga
     4381 gactcaaacc aggataaggt gccatttctt ttgctaattc aaagatacgt cgggcattag
     4441 tcccgtaaaa atcagcgata taagtagcat cttcttcctc taaaccggct gctactcctt
     4501 cttttgctaa ttctgtcact gtttcttcta atttagttgg atcgaagttt ccacctgaaa
     4561 tctgatattt tttagagtcg atttctttcg tctctattcc gtattcttct tttaataatt
     4621 ggcgtatcaa tcgcaaagct ccttctgcca tcttacggta atcggtgatt ttcccaccag
     4681 ataaagtcag caaaccatct ggttctcttt ctaaagaact acctctggaa atcgtagaag
     4741 gtgctttttc atcacggctg ttttccaaat ggttcaacac gtcttctact tcagcacgag
     4801 aaactttgtt ttccttatat tcacttactg tatcgacaac tttattgaag ctcttgtctg
     4861 aaatcgatcc attatctcca ccattgtaat cagaaccaga attaccaatc aacagtggac
     4921 gaagtcctgc ccagcttgct tcgatatcat tcaatgtgat atttgcttct ggatagcgat
     4981 gattgatcac atccaataga taatctacat cttcttgtgt gactttcgga tccgtaaaat
     5041 ctccttggta atccgtatct gtcgtaccaa agtaagtctt gttttctctt ggaatagcaa
     5101 aaaccatccg cccatcttgc tttcctgtat cgaagtatgt gggttgcggt acaggcagtt
     5161 ttttcgcatc tacaactaaa tggatccctt tagttggacg cattttagga gagactggac
     5221 gcgtaaaatt caagtttcgt actttatcta cccaaggacc actcgtattg atcactaatt
     5281 ttgctttgat ttcgatcact tcatctgtca gcaggtcacg agctttgacg ccaacgattt
     5341 gatcgccatc atataaaaag ccaaccgctt tcattttact tactagataa gccccatctt
     5401 cggcagcctt tttgatatta tcaatcacta aacgggcatc attgttgcgg aaatctagat
     5461 agacacccgc gccttttagc ccttcttttt tcaaaaacgg ttctctttcc aatacttctt
     5521 ctggtgtcag cgtataattc tcatatttgg ttcctgtcac atttgccagt ttgtcataaa
     5581 ggtccattgc tactttgacc gagaacatat tgaaggttgt tgctccttcg ccctcataga
     5641 ttggcaaaag cattggatct ggttttggga tgtgtggggc aatactttgt acgactgcac
     5701 gttcaccaac tgtgtcagct actacttcca catcaaatgt cttcagataa cgaataccgc
     5761 catgaacaag tttggtcgag cgagaggaag tcccttctgc aaaatcctgc atttcgatca
     5821 atcctgtttt gattcctgat gctgctgact ggactgccac accggcacca gtgattccgc
     5881 caccgatgat caacagatcc aattcttctt gctgcatttt ttgaatgcta tcttgtcttg
     5941 tcttgtttga aaacatatgg atttcctcct tattcaaatg cttgggtagc ttttactgct
     6001 tttttccaac cgttgtaaag tttttctcgg cgctcttcct ccatttgaac ttcaaatagc
     6061 ttgccagctt cataaaattc gcggatctcg tctgtatctt tccaataacc gacagctaat
     6121 cctgctaaaa atgctgcccc tagcgccgtt gtttccaagt tttctgctcg ttggatcggt
     6181 acattcaaga tatctgtttg gaattgcatc agatattcat tgtttgctgc accgccatct
     6241 actttcaata caggaatctt gattccagtg tcatcttgca tcgtatcgat gatatctctc
     6301 acttgataag caatcgactg taaagtagct ttaatgatat cttcttttgt cgtaccacgt
     6361 gtcaagccaa acatagcccc tcgtgctgcc tgatcccaat aaggagcacc tagcccaacg
     6421 aatgcaggaa caacataaac ctcatcttcg cttgtagagc gtttcgctgc gtcttctgaa
     6481 tcgctagctt tttgcaacat ttgaagtcca tctctcagcc attgaatcga agaaccagcc
     6541 acaaaaatac ttccttctaa agcatagtaa acctttccat ttatgccgta accaattgtc
     6601 gtcaataaat tatttttgga taattgcggt ttttcaccgg tattcatgac aataaatgaa
     6661 cctgtaccat aagtattctt gaccatgcct ggttcgaatg ccatttgtcc gaataatgcg
     6721 gcttgctggt ctcctgccat ccctgaaatt ggtgcttcgc ttccataaaa atgataacct
     6781 tgcgtataac cataaacttc tgagttagaa gtgacttttg gtaacatcac tcgaggaata
     6841 tttagtaaat ctaaaatctc ctgatcccaa tctagctgat ggatattgaa caacatcgtc
     6901 cgactggcat tcgagtaatc agtaacatgt gcttgtccat cggtcagttt ccatactaac
     6961 caagaatcga tcgttccaaa catcaattcg cctttttcag ctcgttcttg cgcgccttct
     7021 acatgatcaa gaatccagcg tatcttcgtt gctgagaaat aggcatcgat cactaaccct
     7081 gttttttcgt ggaaaaattc ttgatagcct tcatttttta agttatctgc aattccagct
     7141 gactgtctgg attgccaaac aactgcatga tagattggac gtcctgtatc tttttcccat
     7201 attactgttg tttcacgctg gttcgttatg ccaatcccag caatctgatt tggtttgatc
     7261 cctgattcga tgaacgctcc ggcaataact gactgtacag aattccagat ttcatttgca
     7321 tcatgttcca cccaactttc ttgcgggaaa tattgcgtga actctttttg agaactgccg
     7381 atgtgtcttc ctttcttgtc aaagataatt gctcgtgagc tagttgtacc ttggtcaatc
     7441 gccatgatat acgttgattc tgtcattgtg attccctcca ttatatgcaa acgctttctt
     7501 taactactct tatcatacta ctgttttaaa aaaagaaact gtcaaaattt tattcttttt
     7561 ttcttttttt gacagttttt tattatgaag ctatttttta atcaattctt ttcttttttc
     7621 agcgtctcta ttcttctttt caatcgaata atgtcgtaag gtgacgcaaa ttctgacaga
     7681 taatattcac actgtttttt gtttctctgg caaaatgtga tcaccaaatc atattcttta
     7741 ccttcttcat aacttccaat ctcaataccg ccgattcccc tcagttctct gatcagatat
     7801 tgttgaaaag caatacgata aatcggcaga ctttctaaat cgattccaat cactactggt
     7861 tttgcaatat aaaaatcgat catcaaaaga acattggcat actggatcat caggtaacta
     7921 agctcactat cttcagctct tttcctcggc aattgctgca ccgctgtttc ctgtaatttc
     7981 gccagtaact gtgttaagga atgcccgagc attttctgct gctgttccaa aagccgatca
     8041 cgctcataga cttcgatctt tccaacaaag aaaaaccatt cattgttgat ctgggaaatt
     8101 tggtagccta ctgccttttc ttctttgatt ttcaaacgac gaggacgata atgccataac
     8161 atcgtttccc gaatgtaagt atctaaaaca gcggttggta actttttgct ccgtgtcaaa
     8221 tcatactgat aaaatatttc ttcgtctact atcgaaaaag aaacaaaaaa gctgtaaaac
     8281 atcattgttt cgtatacatt cagttctgct gctgttcgac taagatgcat cacgatgatt
     8341 ggatcgattg cttgataaag ccggtccgac tgatagagta atttcttttc tttcaaagtt
     8401 gcgtacgtac ttttttcatt caatagtctt tttttcgtaa tcccgagcca gcaagcaatc
     8461 tgtgcttctg ctgatgccgt aaacgttgtt tctaatacac ggtccaatcc tagaatgaac
     8521 ggtctttttt ctggtgtatt ttgcaaaaaa agcggtcgct gatcttccgg gatatattgg
     8581 aatagttcat aataaaaata tcgtatctgt agctcttctc cgtataattg accgtttttg
     8641 atttgtaatc caaattcctc taagagctgg ttcaattctt tgatctttcg aaaaacagaa
     8701 gactcactga tattaaacgc tgtcgctaat cgaacgatgg atacttgctg atgttctaat
     8761 aaatagacga gcatcttaaa cttcaagctt tcctgcaata aaaacaatag aattttttcc
     8821 aaacttacat cttcgtccaa cacaagcagc gcctgatact catttcgctg tacttctact
     8881 tttttttcca tcattcggcc aatcaattca atatcttcca aatattgatc aaacgaggct
     8941 tttgacagtt ggacatgctc tctcaaacca ttgatagagg ctgttccccc ttgcagaaca
     9001 aggtttcgca aaatcaggat ctgtaactgc tcttttcttt ctaataatgc ttcaatcttc
     9061 ataggcagta ttcgccacct ttgaccacac catatctggc acacggtatt ctggctggaa
     9121 aaatagctca tgttcttcta actgttcaaa cgtccatcct tcttctactg caattgtcag
     9181 ttggtcaaga aggtggaaca tctctttttt gctttggagc tgtgctccaa gaatcttttt
     9241 ttcagaatca aatagaaatt tgagtttaac tttgctttga tttattgctg taagcttgta
     9301 gtctacgtat cctgtttcaa ttttttgggg ataaaaaatg ctctcttctt ctgtcaaacc
     9361 tacgcttcct aagaaatagc caaaccactg attgccgact ggacgatagg ttccctgatc
     9421 ctttgtctgt gagccactaa ttgtcttgct gacatttcgt gctgtacgga cagcgttatt
     9481 tactagagaa acatacattt tttcatttgt tggacgaaaa gttacttgga tcgcatcgcc
     9541 aatcgcataa acgtcttttt gagacgtttg caagtagtca tctacccaga tcgttccatc
     9601 atcattcaat gttaattgtt cttcccacat ctgattgtcg ggacgagaat gattagccag
     9661 caaaacaaga tcatagttct cctttctttt ttccgtctct acaatagcgg tttcattttc
     9721 ggtcaaagac acaacttgtt cttctaaaaa aagtgaaagc ccttgatttt tcatttcctt
     9781 tactaacggt tctgtcattt caggatctag ataacgaaaa agaattgact ttcggctttc
     9841 gtaaagatgg atttgttttt ttcttgatgc caacccttca gctatttcga ttcctgcttg
     9901 tcctgcacca ataattgcaa ttttctttgc attcatgatc ttttgctgaa gagccgttac
     9961 gttttgtatc tttttaactg ggtgaataag cgttgattct gcagaaatat ttcgaaactt
    10021 ttgatttgaa ccagtcgcta gaatcaaacg atcaaaatcc aatttttttc ctcctgctaa
    10081 aacgactttt ttttctttca atcctattac tgtcttttct gtgtaaacat ggattcgata
    10141 gcggtcagtc agttcttctt tcgtgatcca agaaagttgt ttttcactgg taacctttcc
    10201 ttttaatatc aaattgaacc cgctgggaat ccatcctaac ttcgattgct tttcaatcaa
    10261 atatatttca cttgttggca tcaattggcg aagataaata gccgcatgaa tccctgcaaa
    10321 cgacccgccg acaattacaa tgttcatctg cattcctctt tcttataagg tgttctactg
    10381 ctaacttctc aatcagaaaa cacagtattc ataactcgct ttcaacctta tgatacatag
    10441 cgatgcagca gaatcaaccc tctccatcat accgtgttct aggtctgctt ctcaataatg
    10501 tactagtgga cctaattgaa gagtatcatt tctgaagaga tgtttcaaaa aatgtactct
    10561 tataaaagaa aaaaactcgc cttttacgta cctctgtcca aataggaaac agaaaagaac
    10621 gaaaagacga gttttagcgt gtgtaatgta atagttagaa tagacacatc atttattttt
    10681 aattctttaa tgcttcaaca actgcttcgc ctaattgatg agcagattga ggattttgcc
    10741 ctgtaagaag ccgcccatca atttgaacca cttctgaaaa agctgctcca gactgatagt
    10801 ttgctccttt ttctttcaaa gcatcttctg ctaagaatgg tacttcattc gttgtaccat
    10861 ttgcttcctc ttcttcatta ctgaacccag tcactgtacg tccagctaaa atagattttc
    10921 cagactcatc tgtaactggc aacaatccta caacaccgtg acaaacagct gagaccagcc
    10981 cttcatttcg atagatttgt gaagcgactt tcccaagatc tattgcttct ggaaaatccc
    11041 acattgttcc gtggccacca gcataataaa ccgctgcata atcatttgca tccacatcat
    11101 caggacgtaa ggtatttccc aaggcccgat ttctgaaatc ttcatcttca taataggccc
    11161 aatcgatttc atccatattc aaaacacttt gcggttctaa aggtacatat ccgcctttag
    11221 gactgacaaa atctgcatcg atcccatttt ttactaatac atcataaaaa tgagtcagtt
    11281 cacttaacca caaacctgta ggacgctcga tcttttcgta cttttctaca tttgttaata
    11341 cgatcaatac tttcattttc ccatcctctt tctataaaaa ttgcgttatt gataacgtgc
    11401 tttcaagaaa ttatataatg ggcttggcaa ataaagctct ttatatgctt cttcaaaagg
    11461 tttcgttaca aattcatcct cacggttttc caataatttc tctgcccagt caggatcgac
    11521 gatcatttgc tgtccaattg ctgcaagttc tgcatatttc aatacctgct ctacatcttc
    11581 gcgattgcgg atcccaccga caccaactaa agggatcttt ccttgaatcg tgttatggac
    11641 cacttcaaga atcgatttat cgttgtattt atcagaacga gctattcgat catacatatt
    11701 taatgaaaca tgcagataat ctagttttga ctcacgtaat cgttcaagaa gccagatcgt
    11761 gtcttcaaaa cggatacctg gtgtttcata ttcttctggt gagaaacgat accctacgat
    11821 gaatggtttt ttagcatatt gttcaaccgc tgagaaaacc gttgttaata attcttcaat
    11881 aaaacgataa cgattttcca agctaccgcc ccattgatcc tctcgtctat tcgaatgcgg
    11941 agagaaaaat tgttggatca aatacgtatt tgctccatgc aactcgattc catcaaatcc
    12001 tgattcaatc gctctacgtg tggctttgcc aaatgcttgg attgtttcaa cgatttcttc
    12061 ttcactcatt gctcgtggtg tttccgcatc aggacgtttt gctgcaactg cacttgcaga
    12121 tacaggctgt tctccagata aagttgctcg acttgtcatt cgtccaccgt ggaaaatctg
    12181 aataatcgct tttgcgcctt ctccttggat tgctttcgct agctcactta atcgaggaat
    12241 catttcatca tgagcgatgc tgagttcacc tggccagcct tttccgatat cttggacatt
    12301 tgcagcacca gtaataacag caccaagacc tttagaacgt tgtttataat aagtgatctc
    12361 atcttcggtg atcactccat tgaaaaagct gagttgtgtc gtcataggcg acagcatcaa
    12421 tcggttcttc aattgaactc ctgaaggtaa agtaattgat tgatcaaatt gtgacattgt
    12481 cattcctccc ataattcgct ctgattaacc tatcgagaat catctatcat taaaccactc
    12541 gattagttgt acatgcaact aatttgctta aaaaatttta ttttttttgt aacttatctg
    12601 ttgcatcatt cattgtctgg gctaattctt tacttgcttc atttcccaaa actgctttgt
    12661 atgactgatg aagttcttcc cagctaccat cgacttcctt agcaaattgg cgtccttgtt
    12721 ctgtaagata aactaatgac aagcgacctt cgctaactgt tttgacgtat cctttatatt
    12781 ctaatttttc aacaaaacga gcaatcgttg atggggcgga atagagcatt tgtgcaatat
    12841 ctttttgtgg tattccatct ttttctttta atatcaataa aataaaacca taagttggtg
    12901 ccagacctgt ccttctaaac gcttcatcag ctaatttatt caacatgcga tctagctttt
    12961 tcacgctaaa gaaaagacat tcatctaacg ctttttcaat tggcttttcc atttcatccc
    13021 ctccattggc gtacatatca tagtatgatt tttatttttt gtcaaaaaac acggaataat
    13081 gtccgcctcc tctgtctaga tttgaaaaaa agaagtagcc atcttcaaag aatttcggct
    13141 tgttttcgac aaaactcctt ctggaacacc gtgaatcaga ggttgtgaaa aagtcgttta
    13201 gttttgagta tgaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttgg
    13261 cttaataccg aaaaactggc ttttgaacac cgtttatatc agaggttgtg aaaaagccgt
    13321 cctgcatcaa gtaataagaa cagccaaaca aaaatagttc ctcatatttt gtcgtttttt
    13381 gagctaatgc cgatggtcag ctgattgtac accgtttatt gagaggtcgt gagcctgccg
    13441 ttgaactccg agtcacaagg agcaatttca aaaaacagct tctcatgttt tatgactatg
    13501 gtgacttgtg atcgaggagt tggcaggaga atactgttta ttcgtaaaga aggggctgtg
    13561 acaagacttt tgtcacagtc ccttatttcc ttttattata ggatttcgcc atttgattgg
    13621 atgacttgtc tgtaccaatc aaatgatttt ttctttaacc gtttctttgt gccattccct
    13681 tcgtcatctt gatcaacata gatatatcca taacgttttg acatttgtga agtagaagcg
    13741 ctgacgatat caatgcagcc ccacgacgta taaccaaata aatctactcc atctttgata
    13801 gcttccttca tctgctcaat atgctgcttc aaatagtcaa tgcggtaatc gtcttgaacg
    13861 gttccatctt cttctacttt gtctatagct ccgagtccgt tttcagctat gaacagtggt
    13921 ttttgatacc tatcatataa ttgattcaaa gcaattctca aacccttagg atcaatctgc
    13981 cagccccatt cacttgatgg aagatacgga tttttaattc ctccagttat taagtttcct
    14041 tggacccctt cccaattttc tggatgagca ctgactgcac tagacatgta gtagctaaaa
    14101 gaaacaaaat caacagggta ggctgctaag atttcttcat cacctacttc tatttttaag
    14161 ttgacacctt tttcttcaaa cattcgttgc gtatacgctg gataatatcc tcttgcttga
    14221 acatcactaa agaacaaaga agttctttgc tgctcaattg ttttttgaat atcatccgga
    14281 tcacaagaat acggaacaag aagctgatag ctgatcatac aaccgacttg tgaacctttg
    14341 ataatttcat gacaagcttt tgtagctaaa gcacttgcta taaactgatg gtgtgcacct
    14401 tggtatttcg cttgttccaa atggggatgg ttttcttcaa ttactcctac gctaacaaaa
    14461 gggtgatgtt ttacacagtt gatctcattg aaagtcatcc aatattttac tttattttta
    14521 tagcgtttga atactgtttc agcgaaacga acgtaaaact gaataagctt tcgattaggc
    14581 catccaccat attctgtaac caaatataac ggcatttcat aatgggaaag agaaacgact
    14641 ggctcaatcc catattttag acattcgtca aacacttgat cataaaaaaa taatcccgct
    14701 tcattgggtg tttcttcatc accatgagga aaaattcttg tccatgcgat agacatccga
    14761 tagcatttaa aacccatttc agcaaataaa cggatgtctt ctttaaagtg atgatagaaa
    14821 tcatttccgt gtcttttagg ataataatac ttgctgttgg gatcctgcgc cgcttttact
    14881 tgctgatgcg tcatcatatg ccattgatct gtccattttt ctttaggtaa atcgctgtcg
    14941 aatgtataca catcagacac agataatcct ttttcatcta catcccatgc accttcacat
    15001 tgattggccg ctgtcgctcc gccccataaa aatccttctg gaaatttttt catatcgatc
    15061 cccctaaact tgctagtttt cttgtttgat tatttttctt atgactagta atgatatttt
    15121 tgaatctact actatttgta acaataacca ttgtggtcgg atcatatccc tctttgatta
    15181 attcttgcaa atctacttcc atcaataaat cgcctttacg tacttttttt ccatcagata
    15241 ctgcactttg aaagtatttg ccatttaaat tcactgtatc aataccgata tggatcagta
    15301 tctctgctcc ttcttcactg actaccccaa ttgcatgttt cgttggaaaa actgttgaaa
    15361 cttccccgtt tacagaactg tagatttttc cgtccgttgg aatgatcgca aacccttctc
    15421 ctaaagatcc actagcaaat gttggatcgt ttacttcaga taaatcaatt atttcacctg
    15481 ctgcaggagt acttaacact atatcctttg aggattcttc ttcgatttca tccttgtagc
    15541 cgaaaagaaa agttagcata caggaactga tcatcgctaa gaaataacta gcaactagcc
    15601 aaacaaatcc ttcaccaata taaatcggga aagacaaaat acttcttgta gcaactgcca
    15661 aagcaccggc tcctgcggca ccagctactc caccaaatat agctccacat atacaagcaa
    15721 tgtaaaatgg tcgtttatat ttaagagtca caccataaac agcaggttca gtgattccaa
    15781 ataatgctgt gataaatgca gataaagata aactttttac tttcttattt ttcgttttca
    15841 aaaatacccc aagtactgct cctgcttgcg cagctacggc agtcatacat gctggaccta
    15901 gcgtatcacg accgtagaaa gaaagattgt tggtcattac tggtacaagt ccccaatgta
    15961 acccaaaaat cactaacact tgccaaaatc cgccgataac agcaccagat aaaacagggc
    16021 taaagttata aacagctaaa tatccagctg caatcagctg acttgcataa tctgcaattg
    16081 gacctacaac taataaaata attgggacca tcacgatgat acaaatgaat ggtgtcaata
    16141 aatttcgaat actttcgtgc aagcttttgc gaatcttagg ctcaatataa gaaagaaccc
    16201 atacagctaa aattgctgga ataaccgtac ttgtatacct tgctagaatc acgggcatcc
    16261 ctagaaatcg caaggttatg ctatcactat atgcactgac aatcgtagga taaacgagag
    16321 ctgctgcaat aaccatagcg atgaaacgat ctgtattgaa cttttttgca gcagtatatg
    16381 ccaaaaatat aggcaagaaa tagaaaaatg catcagctgc tgcattcaaa atgatataag
    16441 ttcctgattc agtagttaat aaattcaaac tcgtacataa ggcgagtact cctttgatca
    16501 aaccacttcc tgccaagacg cctaaagtcg gggcaaaaac agcggcgatc atgtctataa
    16561 aagagtttaa aatgcctttt ttctcagttc ctctctttcc actagatgca tcatcttcta
    16621 gtttcatttg tttggataat acgtcataca cttcatttac tgcatttcct actacgactt
    16681 gaaacattcc tccagcttcg actacagtga tcacaccttc tagttgctct accttctcct
    16741 tatctgcctt cttattatct tttagggtaa agcgtaacct tgttgcacaa tggtaaacat
    16801 tgctgacatt ttcttcgcct ccgacaaatc ggacaatatc tttcgctaat tgtgaatagt
    16861 ccatttttac ttcccccttg ttaattaatg ccgtagcaat ccttcttaca agataagtat
    16921 agttcttaga aaacggtttc tccattcaat tagtacccct tctaatagcg aacgaaactg
    16981 tacaatagtg tgtatggttt acttagccga gcaaatcact cactgcttga aagaaaacaa
    17041 gtatatcgtt tctcttatag tcaaaggaga caaaaaaaac acgcttgatc ttcctttgct
    17101 gatgtataag tggtatctca agctgatact ctgtgatttt tgtttctact tggcttttga
    17161 taagacagca caacaaagtc tcggcattta acggaagcat aactaggtca gaactaggag
    17221 agattagttg ctgaatggaa tctatctttt tatggatttt tatttgattt tcttctttgt
    17281 agtaatgatc aagcagtttt gttttctttt tttcttcaac tatggtcagc ttttcacgca
    17341 aaatctgata aatctcccaa gattcaaact catctagaac actaggtaga taaatcaaat
    17401 catcccactc aaaaataagc tcttgctcag tagtcaaaat tgcatcatat tccgttattt
    17461 tgtttgattg atttttgaaa agatacactg gttctatccg gcattctttg atccacatgt
    17521 tcatactttg ttgaatctgt ttttttagtg tattcaaaat actaattggc tggttactaa
    17581 caatcagtat ctccccttgt tttcgaaaag gattcaacgc ttcggcaaga tacaaaacaa
    17641 aataagcgct ttcagctaaa ttgggacgta attgaaaatg tattgggaag aaacgccgaa
    17701 ttaaataaat ttctaaagga taacgggaga tgatcttttg tacaaaatga ttcatggtat
    17761 tatgtccggc atccatcctc gttttcattt gtttgatatg ggttagcaaa aattctgttt
    17821 catggaacaa taacgaaaca ggaagatcta aaaaacgaat aatcgtttgc tcaaatgctc
    17881 ttagattctt ctctatttct ttgttttttt catcattaat catcaaataa tttaactcta
    17941 atagtcgatt ttcaattgct tgtttttctt gattggaaaa agaatagggt agttctgatg
    18001 ataacttttt tgtgagggtc tctaccatcg gaataaataa tgtatacctg tttgtttttt
    18061 ctaaaagttc ttctcttcga agaatggtca aaacgagata ccgagaaaat cgtatgaagt
    18121 cctctcctga aacaatataa ttatatgata taagaacctc ttttaatgtc ttttggacag
    18181 tcggcatgat attggaaaaa attgtactga tttgttgagg aaaccttgcg tgttggatca
    18241 ccgtttctgt tccaacaaga gataagaaaa ttctttttga attttcttca gcaatgattt
    18301 ttagtccttt tgtcgacgaa acttccagtt ctaatttagg attgcgctcg atccaacgca
    18361 aaatcgtttt tatttgtaat gatacggctg tttttgaaag aaagaaaatg tctgctatct
    18421 tttgcatact gacataccct ttttgaaaca ataagtacgt cacaatagtt gaagctcgga
    18481 aatccatgct ggaatagatt ttatgtttcg tcacctcttg tatgatttgc tgtttagttt
    18541 cagattcaat tgattctaaa aaataacctg aacgtttatt tgactggata atgattttct
    18601 tctcgtagat agcaagcata tttttgattt cagtttggat cgttttcttg ttggtaccta
    18661 aagcttcagc taattcttcg cttgttatcc aatgattctt atttcttaat actacaagaa
    18721 tttggagttg tcgtttattt aagtccattt gcttacacct cctctttgtt ataaacatac
    18781 aggaaaaacc tctcaaatga caatagtcac ttgcagaggt ttaaaaatca tttgaagaaa
    18841 gatctatatt caaggttttt accattagac tggataattt gtttataata ttcaaaacta
    18901 tcttttttca atctcctaaa gcttccgctc ccatcatttt gtctatccac ataaataaag
    18961 ccatatcgct tcctcatttc ccctgttcct gccgatacga tatcgattgg tccccaccat
    19021 aagtaaccaa tgatttggca accatctttt attgcctcag caatttcctg aagatgtatt
    19081 tttagatact cacaacgttt tggatcgtgg attcgattca attcatctgg tgcttcatct
    19141 agtccaatgc cattttcaac tatgaataat ggtaattcat aacggtctgt caattcattc
    19201 atgacataac gaaaccctag aggatcaatt ggccaactcc aaggttcggg agatttttcc
    19261 ttcaaaaagg gattatcgaa accatttgct ccgccagtat ctacgcgcat ttctgcttct
    19321 tttttataga cagcgctacg ataataacta aatgcaatat aatctgccgt gttagcttgc
    19381 aagcactgtt cgtctccatc taacattact ggtgcacagt cttcttcctt ccatgtcctg
    19441 taaatataag ctggataatg ccctcggcac atgacatcgg agaaaaacca tgaattgcgt
    19501 ttaaattgca gtgaaccaaa tacattctct ggatcacagt catatgcata agtcgctaat
    19561 gcagaaaagc tacacataat acccatctta gcatttggat cgattttctt taataattga
    19621 gtagcacggg cgttcgctag aaacatatga tgacaggctt gataaatcac tttttttgtt
    19681 agtgtagcaa tgggttcaag tttatctcta gcagggaatg taaccaatcc agcagctgca
    19741 taaggcatac gaaaagcatt attgatctca ttaaaggtca tccaatattt cactttccct
    19801 tggtagcgtt caaagacggt ctgcgcataa aattcaaaaa agtcgattaa ttttcgattt
    19861 tgccaccccc catactctcc taccaatgct aagggggttt cataatgact aagagtaatt
    19921 actggctcga tatcatatcg atttaatgta ttgattagtc tgtcataaaa tacgagacca
    19981 gcctcatttg gcttctgttc gtctcctctt ggaaatattc gtgcccatga gatgcttgtt
    20041 cgaaaagctt ttaaacctaa ctccttcagt aaacgaatat cctcttcaaa tcgatgataa
    20101 aaatcaatcg cttcatgaga aagataatgg agtgattcgt ctaaagcaat ctcccaaatc
    20161 tttttttctt cattccactg aatactagga tgctggttca tgatcccgat aagcgtatct
    20221 gtcacattag gtaatttgcc gtcttccaac catgcacctt cacattgatt agcagctact
    20281 gctccgcccc ataaaaaatt ttctggaaat atttcctgat ccataactat ctctcctcgc
    20341 tatcaacaat ttcttacttg aagtatagct cgattcatca aaacaagata ttctaactat
    20401 ttcggcagaa gagaagaaaa aaattgaatg ctactcttca tttttaatat ttatatataa
    20461 caaatacata agacgatccc cgaaagaaaa cactcgttaa ccatctaagc aaacgattta
    20521 ttttacttcc aacacaaatg cttcataagg tcgtaaatca actttgccgt ctggtaactg
    20581 tttcagagga taatttgaca caataaccct taaagcttca cgtggaatag tgaacgtctg
    20641 cttttcactg gacatattac aaataacgag ccattgctgg tcttcatata tgcgagaata
    20701 ggcaaagaca tgctcaggcg tcttttccac taattcatag tctccccaga cgatgatgtc
    20761 atgttttcta cgcaaatcga tcaatttctt gtacatataa aaaacagaat cttgatctgc
    20821 taacgcttct tctgcattga tttctttata atttggattg actcgaagcc atggaattcc
    20881 agtagtaaac ccagcatgtt cacgggcgtt ccattgcata ggcgtcctag cattgtctct
    20941 tcctttcgcc ttgattgatt ccaagatttc ctcttttgta aatccagaag aaatcctttc
    21001 gttatacata ttgattgtct caatatcttt tgcttccgcg atatcatcga ttgggcaatt
    21061 cgtcatgcct aactcttctc cttgatagat ataaggggtg cctttcatca tatggagcaa
    21121 gatagcaaac atcttggcac tttcttttcg atacacatcg tcatttcccc aacgagaaac
    21181 aattcgcgga agatcgtgat tattccaaaa taaactattc catccttcat cacctaaaga
    21241 tgtttgccat ttggataata cttttttcag ttcagagata ctcaagggtt tcaaatccca
    21301 tttgtctttg ccttcttgct gatctaatcc tatgtgttcg aattggaaga ccatggataa
    21361 ttcatttctt ttaggattgg aataaagttt agcgatttcg gtagttgctc cccatgtttc
    21421 gccaacagtc atcagatttt tgtcaccaaa agttgcttga ttcatttcct gaagatactc
    21481 atgcagttta ggtccattac ttgtgatttc tttatccggt tctttgccaa tcatatcgat
    21541 cacgtccata cgaaagcccc caataccttt atcgatccag aaattcatca tttgataaat
    21601 ctcttggcga acctgtttat ttttccaatt caaatcaggt tgacgcttgc tatacaagtg
    21661 cagaaaatat tgccccgttt tttcatccaa ctgccaagca gaaccactga atattgagcg
    21721 caatctatta ggctcttttc cattgaccgg atcacgccaa atataatagt cccgataagg
    21781 attttcttta tttttacggg cctcaatgaa ccacggatgt tcatcagaag tatgattcac
    21841 caccagatcc atgatgatac ggatatttct tttctttgct tcttcaatca gttcttccat
    21901 ttcttccatc gtaccgaatt ctgccatgat tgcttgataa ttactgatat catacccatt
    21961 gtcatcattc ggagactcgt atactggaga aagccaaatt gcatcaatac ctaaatacgc
    22021 cagataatcc aacctggaaa taatcccccg caaatctcca ataccatctt gatttgaatc
    22081 ttgaaaactt cttggataga tttgataaac aactgaatgg tgccaccatt tttgttccat
    22141 gattttcctc ctataaatgg atgtttctat tttttgctta atcatttatt ttagtctctc
    22201 tcactaaaaa ttgtaacgct ttcaaatagc tattgcacct tgttatcggt aacagaataa
    22261 aacaaaaaaa cagctgagga aattccctca actgtttttt gaaaggattg atttatgcag
    22321 ggaagaatgc tgatttcaca acttctttat aaacggtgtt cactcagctg tcccttggta
    22381 ttagctcaaa aaacggcaaa atataagaaa ctattttttc gtttttttct ctaatactca
    22441 ggccagaacg ctgatttcac aacctcttta tttcacattc aatatccaca tgaatcccac
    22501 aaatacaaag aacagaccat acatgattgg agatacttct tttcctcttt tagctgctaa
    22561 catggtaatt ggatagaaga taaaacctaa tgcaatccca tctgaaatac tataagtcaa
    22621 aggcatgccg atcaagatca agaatgaagg gatcgcaatc tctaagtttc cccattcgat
    22681 ttgtcgcaaa gattgtgcca tcaatactcc taccataatc aatgctggtg cagttacttg
    22741 tgaagtaaca actgatagta atggagaaaa gaacaagcct aacaagaaga aaatccctgt
    22801 agtaatagct gtgatacctg aacgtcctcc aacggcgata ccagctgatg attctacaaa
    22861 tgcaccaaca ggagaagtac caaataagga acccgcaagc atagctgttg aatcagaagc
    22921 taaagctttc cctacacgag gcatcttgtt gtctttcatg aaacctgctt ggtttgctaa
    22981 tccaactaaa gttcctgctg tatcaaagaa agtcacaagc aagaatgtca gcactacgac
    23041 ccacatttga agagaattga tgtcccctac atgttttaat gcaactaaaa atgttggttt
    23101 caaacttggt gctgcggcaa caatttcaga aggcattgga atcaaacctg tgaccagacc
    23161 taaaatcgtt gtagctgcca tcccgatgaa gatgccacct ggtacacgcc gaacaagcaa
    23221 gatagcagtt acgactaatc caaaaatcgt caaccaagta gaaccaacgt tgagtgatcc
    23281 taatcctact aatgtagatt cattcgaaac gatgatccca ccttcgctca gaccaaggaa
    23341 agcgataaat agaccgatac cgccagaaat cgcatatttc aaatctgctg ggatcgcatc
    23401 aatgatcatt tcccgcagct tgaagattgt gatcaaaata aaaatcaaag aagcaacgaa
    23461 tacgccggct aatgctgttt cccatggaat ccccattcct aaacaaacag aataagcaaa
    23521 aaaagcattg atcccaagag caggtgccgt tccaattgga taacgggcta atacccccat
    23581 taaaacacag ccgatcgcac tggctaatgc agtcgcagta aacactgctc cttcatccat
    23641 tccagaagca cctaatacag ttggatttac aaataagata taagccattg agataaatgt
    23701 tgtaaatcct gccagtacct cacgttttac cgtggtgttc aatttttcta tttcgaagta
    23761 ggaaagtatt ttttccttca tcttccatcc ccctaaatcc gaacattata aataaagtcc
    23821 gttaaaattt cgatttatta ataaa
//
LOCUS       DANKHL010000018        23830 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000018 DANKHL010000000
VERSION     DANKHL010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.020"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(54..788)
                     /locus_tag="PW101_000510"
     CDS             complement(54..788)
                     /locus_tag="PW101_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL2512940.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="PW101_000511"
     CDS             1115..1525
                     /locus_tag="PW101_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDL2512941.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="PW101_000512"
     CDS             1518..2219
                     /locus_tag="PW101_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDL2512942.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="PW101_000513"
     CDS             2219..3805
                     /locus_tag="PW101_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2512943.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="PW101_000514"
     CDS             3824..4045
                     /locus_tag="PW101_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDL2512944.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="PW101_000515"
     CDS             4042..4803
                     /locus_tag="PW101_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HDL2512945.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="PW101_000516"
     CDS             5183..5692
                     /locus_tag="PW101_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL2512946.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="PW101_000517"
     CDS             complement(5767..6306)
                     /locus_tag="PW101_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDL2512947.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="PW101_000518"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="PW101_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDL2512948.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="PW101_000519"
     CDS             7319..8170
                     /locus_tag="PW101_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDL2512949.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="PW101_000520"
     CDS             8198..8833
                     /locus_tag="PW101_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDL2512950.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="PW101_000521"
     CDS             8853..9626
                     /locus_tag="PW101_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2512951.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="PW101_000522"
     CDS             10034..10831
                     /locus_tag="PW101_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDL2512952.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="PW101_000523"
     CDS             10809..11222
                     /locus_tag="PW101_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2512953.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="PW101_000524"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="PW101_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL2512954.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="PW101_000525"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="PW101_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDL2512955.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /gene="maf"
                     /locus_tag="PW101_000526"
     CDS             15999..16559
                     /gene="maf"
                     /locus_tag="PW101_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDL2512956.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="PW101_000527"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="PW101_000527"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDL2512957.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="PW101_000528"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="PW101_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDL2512958.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="PW101_000529"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="PW101_000529"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDL2512959.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="PW101_000530"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="PW101_000530"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDL2512960.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="PW101_000531"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="PW101_000531"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDL2512961.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="PW101_000532"
     CDS             21907..22485
                     /locus_tag="PW101_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2512962.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="PW101_000533"
     CDS             22537..23019
                     /locus_tag="PW101_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDL2512963.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="PW101_000534"
     CDS             23136..23510
                     /locus_tag="PW101_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512964.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4286 c         5115 g         6648 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgac
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcaccgggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact cctttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt
//
LOCUS       DANKHL010000019        23653 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000019 DANKHL010000000
VERSION     DANKHL010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.021"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(26..601)
                     /locus_tag="PW101_000535"
     CDS             complement(26..601)
                     /locus_tag="PW101_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2512965.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(606..1580)
                     /locus_tag="PW101_000536"
     CDS             complement(606..1580)
                     /locus_tag="PW101_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleosidase"
                     /protein_id="HDL2512966.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1604..1648)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1863..2831
                     /locus_tag="PW101_000537"
     CDS             1863..2831
                     /locus_tag="PW101_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2512967.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2928..3221
                     /locus_tag="PW101_000538"
     CDS             2928..3221
                     /locus_tag="PW101_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL2512968.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3339..3575
                     /gene="secG"
                     /locus_tag="PW101_000539"
     CDS             3339..3575
                     /gene="secG"
                     /locus_tag="PW101_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDL2512969.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3678..4433
                     /locus_tag="PW101_000540"
     CDS             3678..4433
                     /locus_tag="PW101_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2512970.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4436..6793
                     /gene="rnr"
                     /locus_tag="PW101_000541"
     CDS             4436..6793
                     /gene="rnr"
                     /locus_tag="PW101_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDL2512971.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6815..7279
                     /gene="smpB"
                     /locus_tag="PW101_000542"
     CDS             6815..7279
                     /gene="smpB"
                     /locus_tag="PW101_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDL2512972.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7581..8300
                     /gene="atpB"
                     /locus_tag="PW101_000543"
     CDS             7581..8300
                     /gene="atpB"
                     /locus_tag="PW101_000543"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDL2512973.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8353..8568
                     /gene="atpE"
                     /locus_tag="PW101_000544"
     CDS             8353..8568
                     /gene="atpE"
                     /locus_tag="PW101_000544"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDL2512974.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8659..9183
                     /gene="atpF"
                     /locus_tag="PW101_000545"
     CDS             8659..9183
                     /gene="atpF"
                     /locus_tag="PW101_000545"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDL2512975.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9170..9712
                     /locus_tag="PW101_000546"
     CDS             9170..9712
                     /locus_tag="PW101_000546"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDL2512976.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9735..11291
                     /locus_tag="PW101_000547"
     CDS             9735..11291
                     /locus_tag="PW101_000547"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDL2512977.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11305..12207
                     /locus_tag="PW101_000548"
     CDS             11305..12207
                     /locus_tag="PW101_000548"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDL2512978.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12230..13636
                     /gene="atpD"
                     /locus_tag="PW101_000549"
     CDS             12230..13636
                     /gene="atpD"
                     /locus_tag="PW101_000549"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDL2512979.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13650..14072
                     /locus_tag="PW101_000550"
     CDS             13650..14072
                     /locus_tag="PW101_000550"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDL2512980.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14541..14771
                     /locus_tag="PW101_000551"
     CDS             14541..14771
                     /locus_tag="PW101_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDL2512981.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14868..16169
                     /gene="murA"
                     /locus_tag="PW101_000552"
     CDS             14868..16169
                     /gene="murA"
                     /locus_tag="PW101_000552"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL2512982.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16182..16358
                     /locus_tag="PW101_000553"
     CDS             16182..16358
                     /locus_tag="PW101_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL2512983.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16493..16789
                     /gene="yidD"
                     /locus_tag="PW101_000554"
     CDS             16493..16789
                     /gene="yidD"
                     /locus_tag="PW101_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDL2512984.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16853..17320
                     /locus_tag="PW101_000555"
     CDS             16853..17320
                     /locus_tag="PW101_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2512985.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17405..18289
                     /locus_tag="PW101_000556"
     CDS             17405..18289
                     /locus_tag="PW101_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2512986.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18403..18639
                     /locus_tag="PW101_000557"
     CDS             18403..18639
                     /locus_tag="PW101_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HDL2512987.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18727..18807
                     /locus_tag="PW101_000558"
     tRNA            18727..18807
                     /locus_tag="PW101_000558"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18761..18763,aa:Tyr,seq:gta)
     gene            18814..18885
                     /locus_tag="PW101_000559"
     tRNA            18814..18885
                     /locus_tag="PW101_000559"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18846..18848,aa:Gln,seq:ttg)
     gene            19011..19181
                     /locus_tag="PW101_000560"
     CDS             19011..19181
                     /locus_tag="PW101_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512988.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            19401..20108
                     /locus_tag="PW101_000561"
     CDS             19401..20108
                     /locus_tag="PW101_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2512989.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20167..20811)
                     /locus_tag="PW101_000562"
     CDS             complement(20167..20811)
                     /locus_tag="PW101_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDL2512990.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20938..21225
                     /locus_tag="PW101_000563"
     CDS             20938..21225
                     /locus_tag="PW101_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512991.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21333..21653
                     /locus_tag="PW101_000564"
     CDS             21333..21653
                     /locus_tag="PW101_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2512992.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            21971..23047
                     /locus_tag="PW101_000565"
     CDS             21971..23047
                     /locus_tag="PW101_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2512993.1"
                     /translation="MFDQPEAKPREVVFFWTGQLTEKQTEIAKRILCHSEKRHHLIWA
                     VTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNELFLRYRYVFPEEKITLLHGNTA
                     TAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYNGDAGLAHAVQTAIRSNGRFIY
                     LSATPDEKLLKEIKQTFELHKLALRFHRRLLPEPVLVFMNNWRQKCGNKKKINSLVRI
                     IQQLIKENRILLFCPSITMMHRLKITLQEAFPEYEITDVSSQDDQRAKKVQKMREAIY
                     EILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQIAGRADRKGPYTNSRVYFFYE
                     EKTSAIRKACQEIKEMNEEGKQML"
     gene            23044..23653
                     /locus_tag="PW101_000566"
                     /pseudo
     CDS             23044..23653
                     /locus_tag="PW101_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
BASE COUNT         7820 a         4125 c         5092 g         6616 t
ORIGIN      
        1 attttgctaa tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt
       61 gtggcaacat agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt
      121 ctgtacctgc ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata
      181 gctgaacgat catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga
      241 tcgaagaaac gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc
      301 tgtttttatc tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa
      361 tttggattgg cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc
      421 tggacaatcc tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc
      481 cgatagaatc gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat
      541 tgatcccaat acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca
      601 ttttgttagc ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt
      661 aaaagaacgg ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta
      721 ctgttagcag gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt
      781 ccttgagttt ccacagcagt gaagcttttg aaaccttcac agatggcctc ctcgataaaa
      841 taagcaacag ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg
      901 aagtcaaaat aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga
      961 cagcagtatt cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc
     1021 atttctatcg tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag
     1081 taattgtatt cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag
     1141 acaaaacgtt ccatatgttt cccaatattt gggttgactt tcaaagcaga agcgatattg
     1201 gtcaaaggac ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa
     1261 aaatcaacgg catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc
     1321 cctaaaccat ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt
     1381 tccattcctt gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca
     1441 ttctgcgtac ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg
     1501 atatcagggg atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa
     1561 tcaataatta cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta
     1621 ttctgggaag tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag
     1681 tatactggct tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac
     1741 tttaagtaaa tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc
     1801 ctatggatcg gtacattgga taatagatag attatagttt ggcagaaagc ggcgggtgta
     1861 caatggttat tttttatttc attcttattc tgataattat agggatcagt taccattttc
     1921 tttcttattt ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt
     1981 atgaagacac tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac
     2041 gacaaaaatt agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca
     2101 aaagttatga cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata
     2161 aatgggtcat ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag
     2221 gtaaaagata tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag
     2281 aaagtgaagg agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga
     2341 tccaactcat cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg
     2401 gagcttctac gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta
     2461 ttttagacag cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca
     2521 ctgtctttcc taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg
     2581 gttactcctt aaagcaagca tcagctacga gacagcttgg cagcaatcat ctgcctttat
     2641 tagtcatcca tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattcaaa
     2701 atgctacggc aggggataaa gcactgctgc ttgttccgga agcagaacac ttggaagcat
     2761 cgatgaaaga tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa
     2821 cgatgaatta attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag
     2881 atcaggagac tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg
     2941 catatggaaa tcaaagaaga aaaaatcgtc ttgctttatt caatgatgaa caacaagaga
     3001 tcggagaaat gacatggtca gatgcgggcc cagacatcat gatcgttgac catacatttg
     3061 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
     3121 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
     3181 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     3241 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     3301 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     3361 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     3421 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     3481 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     3541 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     3601 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     3661 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     3721 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     3781 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     3841 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     3901 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     3961 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     4021 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     4081 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     4141 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     4201 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     4261 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     4321 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     4381 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     4441 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     4501 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     4561 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     4621 agtgaaactt cctttaaaac caatcctgat cgaaggaaca tttcgagcaa atgaacgagg
     4681 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     4741 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     4801 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     4861 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     4921 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     4981 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     5041 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     5101 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     5161 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     5221 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     5281 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     5341 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     5401 tgtatatttg actgatcgag taattccgat gatcccgcaa cgtttatcga atggtatctg
     5461 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     5521 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     5581 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     5641 tcagctcgta cctatgttcc aagaaatggg tgaattgcac cgaatccttg aaacgatgcg
     5701 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     5761 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     5821 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     5881 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     5941 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     6001 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     6061 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     6121 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     6181 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     6241 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     6301 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     6361 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     6421 gggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     6481 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacagaaag tgcgagtaaa
     6541 agtagtcaaa gctgatccag atgaacgagc agtagatttt gaacttgttt ccgcagaaga
     6601 ggttacagcg ttggaacggc caaaaacacg aaatggaaaa tcaggaaaac gcgaaggaaa
     6661 acaagcagga aaacgcccag aaagaaatgg caagaagaac aaacgatata gcgatagtaa
     6721 gcaaacgttc ggtaagaaaa aagggaagaa gccattttac aaaggtgtta aaaagaagaa
     6781 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaattaat
     6841 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     6901 ggtcttgcaa ggaaccgaga tcaagtcgat acgaaacagc cggatcaatc taaaagatgg
     6961 atttattcgc gtccgcaacg gagaagcttt cttgcataat gttcatatca gtccttatga
     7021 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaaaaa
     7081 acaaatcatc cggcttgaaa atgaattgaa aaatactgga atcactgttg ttcctttaaa
     7141 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcgaaag ggaaaaaatc
     7201 ttatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     7261 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     7321 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcat aggttttttc
     7381 ttagatcagg agcaccgtgt ataaagaaaa attcgcttcg tttttgactg aaataaaaaa
     7441 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     7501 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     7561 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gttccacatt ggaccaattt
     7621 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     7681 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     7741 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     7801 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacatcggtt
     7861 tagtaacaaa aatcgtttta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     7921 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     7981 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     8041 ttccgatgaa actgattgaa gaatttacaa acttattaac acttgcttta cgtctttatg
     8101 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     8161 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     8221 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     8281 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     8341 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     8401 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     8461 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     8521 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     8581 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     8641 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     8701 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     8761 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     8821 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     8881 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     8941 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     9001 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     9061 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     9121 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     9181 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     9241 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     9301 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     9361 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     9421 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     9481 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     9541 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     9601 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     9661 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     9721 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     9781 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     9841 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     9901 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     9961 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
    10021 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
    10081 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
    10141 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
    10201 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
    10261 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
    10321 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
    10381 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
    10441 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
    10501 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
    10561 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
    10621 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
    10681 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
    10741 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
    10801 cggttgatgc cggactatct gtatctcgtg taggaggttc tgcgcaaatc aaagcaatga
    10861 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
    10921 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
    10981 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
    11041 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
    11101 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
    11161 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
    11221 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
    11281 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
    11341 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
    11401 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
    11461 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
    11521 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
    11581 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
    11641 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
    11701 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
    11761 tctgatcagc caagctttga tgaagtccgc aaaattgtaa atatggcaac aactatgttc
    11821 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
    11881 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
    11941 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
    12001 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
    12061 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
    12121 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
    12181 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    12241 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    12301 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    12361 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    12421 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    12481 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    12541 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    12601 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    12661 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    12721 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    12781 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    12841 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    12901 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    12961 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    13021 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    13081 attgcaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    13141 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    13201 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    13261 ggcttcttct tcaagtgctt tagcacctga aatcgtggga gaagaacatt ataaagtagc
    13321 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    13381 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    13441 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    13501 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    13561 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    13621 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    13681 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    13741 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    13801 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    13861 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    13921 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    13981 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    14041 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    14101 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    14161 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    14221 tggaacaccg tatatcagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca
    14281 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    14341 tgaacaccgt ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt
    14401 tgcatatttt tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat
    14461 acttgttttt ccttttatac attttatggt attatcattt tattagttat tataaaacaa
    14521 tcgttagaaa ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga
    14581 gtcatttggc gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt
    14641 ttttcagacc aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt
    14701 tagggtacac tgcaagtaat tttttcttag aggttatcgc tttatgccga aatcttttcg
    14761 ttagcctgta aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa
    14821 gattttgagt aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca
    14881 tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg
    14941 ttttgcctat tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg
    15001 ttccgattct ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg
    15061 tagattttga tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg
    15121 aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac
    15181 ttgccagaaa tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac
    15241 caatcgattt gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg
    15301 gctatatcga agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta
    15361 gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca
    15421 ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg
    15481 gcgctcaagt ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac
    15541 atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg
    15601 ctgcaatgac acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat
    15661 tgatttctaa attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg
    15721 tcatcggacc aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc
    15781 ctacagatat gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtgg
    15841 tgactgaaac agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg
    15901 ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg
    15961 cagaagtata tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag
    16021 caaatggaat cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc
    16081 atatcaaact acagcaatta ggtgcagacg tcgaacgtgt agacatggat caaacttctg
    16141 ctgaaaaaac agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata
    16201 tcttattaac tttgataaag attttagtgg tgatcttatt gcttatcctt ctttttgtgg
    16261 ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtattccagc
    16321 caagtttgtg gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc
    16381 tagtcgatat gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt
    16441 gacgttagtc gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa
    16501 gatattgatt tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag
    16561 ctgccgttat tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggtgc
    16621 gctaaaaggg acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg
    16681 cggtatcgat tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga
    16741 accagattat aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaaaagagg
    16801 aaatttattg tcaaagtaac aactccgcaa ctacttatat gggggacaaa aaatgatcga
    16861 agaactaaac aagaaagatt taccatggtc cttgttatta gatgcagatc ctgataaaga
    16921 aaaagttcaa gtatatgttt ccagagggag tggctggata tggaaagaaa aagaaaaaac
    16981 aataggtgta ctgatctatg tggcaaggga aaaagaatac gaaatcgtca atgtagcagt
    17041 aaccccaacg tatcaaggaa aggggattgg cggaaaatta cttgagacag cttttcagaa
    17101 gctttcacag ctagcttcaa accaaactcg gatcattata cgtactggca ctactagtag
    17161 cgctgcgtta cacctctatc aaaaaatggg ctatgtggag attggtcgag tcaaagacta
    17221 ttttatccat cattactctg agcccatttt tgaaaatgga gatcaattaa gggatcaagt
    17281 gattttagca attcacttac ctaaaaatag ctttaactaa taaactttca attcgagaaa
    17341 atgctttcaa atgctaaata gatacattac aatagcttta gcaaacaaaa aggagaatat
    17401 ttttatggga atattagttt ttgattttgg tggatcggct gtcaaatatg gttgttggga
    17461 tggcaaagag attaaaggta agggacaatt tgcaacgcct gaatcttggg aagaaatgaa
    17521 aacgcaatta tttcaagttt ataaaaaaat actcgtttct tttgaagtcg aaggaattgg
    17581 aatcagctct cctggagtag tgaataatga gaaacaggtg attgagggaa tcagtgcgat
    17641 tccttatatt catggattta atattttccg agatttggaa actctgtttc aactcccagt
    17701 aacgattgaa aatgacgcca actgtgccgg aatggctgaa ttttatgaag gtgctgcccg
    17761 tgattatcaa gatgttgctt ttgttgtggc aggaactggg attggcgggg cgttgttcac
    17821 gaaaggacaa ataaataaag gggcacatct ttacggtgga gaatttgggt tgatgttctt
    17881 agatggcgat aagacattca gtaaacttgg tacagcggta cagatggctt ggcgttattg
    17941 tgaacgcaaa ggtctcgata aaaatgctta taccggaaaa gatgtctttg aattagcaga
    18001 aactggagat gcaatagcaa aagaagaagt tgagagtttt tatacgtatc ttacaaaggg
    18061 attattcagt attcaatttt cgtttgatcc agaagtgatc gtattaggcg gcggggtttc
    18121 tgcaaaagaa gggctcattg atgagatcaa aagccgaatg aagaagctga cagaacaatt
    18181 cgatctgcat gatttcgaac cgcagatttt attatgtgag tacagaaacg atgctaatct
    18241 tataggtgca gcagcaaact atctcgctgt acgtcaagga gagaattaag agctttagag
    18301 aaggtgcagc ttgcataaaa gaagagaatg aaatgaatag gagattttta cctaaggaat
    18361 gttttatttg ctgcagtatg ataaaatatc tgagaggtga aaatgatgtc aaaaaaaaat
    18421 aaagatattg aagtgcgtat agaagaagtg aaaaaagata tcaaaggtaa gaattatgaa
    18481 gtcactcagt tatttatagg gaaaaaaatg attggtgaga tcttagccta tgggccaaaa
    18541 gaatatgaaa ttttctttgg tgaagaagat tttggaaaag aaaaaagtct tgaaaatgcg
    18601 atagaaacag tgattcgaca ctggaatctg cacgaataaa aaaatatcaa aaaaactttc
    18661 aataaagggt tgcaaaaatc tggaaaatta ggtattataa ctaagtcagg ttgatacaaa
    18721 cctttcggag gagtagcgaa gtggctaaac gcgacggact gtaaatccgt tccttagggt
    18781 tcagtggttc gaatccactc tcctccattt cattggggta tagccaagcg gtaaggcaag
    18841 ggactttgac tccctcatgc gttggttcga atccagctac cccagtatac cacaatcgta
    18901 agctctcgtt agttcgagag ctcttttttt ttgctttgct accttgttcc ttgaagattc
    18961 tgctgaatta agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga
    19021 aagaaaaagt agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca
    19081 atattgaagt gaatattccg aaaaaaggcg gaaaagcggt ccaggtaaaa tcaaaacgcc
    19141 caacgaatca tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata
    19201 agtcggcatt ttcatcgatt ttagggtctt ttgttatttt ctaggttgaa catatctaaa
    19261 aaattgtttc tgacaatagg agtagggaga acgatgtgtg tctaaatcct ctgttgatgg
    19321 atagaaacct ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa
    19381 cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc
    19441 aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa
    19501 gagaattagc agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact
    19561 tggcagatga aggaatctta gaaagaaaga tcggttcggg tacgtatgtt gcgcgagaaa
    19621 aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc
    19681 gagtaccgtc tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg
    19741 aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag
    19801 atgatgtccc tatttgtttt gaagttgcca gtgttccaca aaagctgatc gatggctaca
    19861 gcaaagcaga gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga
    19921 tcggtccagc gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt
    19981 tagaggtaaa acgaagagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg
    20041 gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg
    20101 aaaaataaaa aacaggtgat ccctgattcc ttattagaaa taaaagagaa tcagaatcac
    20161 ctgtttttat atatgatcag ttatcagttg ctcgtgataa gaaacagaac cctcttcaaa
    20221 ttggacttgt ccgttcagca gctcaatgat ggattcttta aagtggttag cacttttttc
    20281 atctacagca cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg
    20341 ttggagttca agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat
    20401 ctcctgttgc aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg
    20461 agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac
    20521 tactgcagct acattgatca gttgattttt tttgaggact tccaacatcg gtacgcccgc
    20581 cgttccactt ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat
    20641 gaaggcgctg cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaatt
    20701 ttttgcttgg tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat
    20761 ctcaatctca ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc
    20821 tctttttttc attttcttta ttcagtataa ataaagttct aggttgtttc aacactgtaa
    20881 cggttgcgca attgctgtag tatgcgataa taaagacaaa ggcaggtgga tctggggatg
    20941 caggaacgaa tgaaaaagta cgatgcaatt acgcattatt tgaagaacaa tggcggctct
    21001 caagtaacgt tgacctttac ccaatttgac gaactgctgt ttcccagtaa cggacttccc
    21061 aaaacagcaa gggaaagtac agattggtgg gcaaatgatt ataaacaccc tgaaaaaggt
    21121 gcctatggct ggataaatgc aggctatgaa gtagttgtca tcaatttgga caaagaatat
    21181 gtcgtgttca ataaattagt gaaatctagt tggctatttg attaaataaa ccgcgtttag
    21241 aaaacgttga caaatttctt tctttttata taataaagta acagacgcga agaaaacgtt
    21301 tgcgtagttt gttttaaaaa ggagagaaaa atatggctaa aaaaacaatc atgcttgtat
    21361 gttcggctgg tatgagcaca agtttactag taacaaaaat gcaaaaagct gctgaagcaa
    21421 aaggaatcga ctctgatatt ttcgctgtgt ccgcttctga cgcagataac aatcttgcaa
    21481 ataaagacgt ggatgtgttg ttattaggcc cacaagttcg ctttatgaaa gctgattttg
    21541 agaaacgtct ggaaccaaaa ggcattcctt tagatgtcat caatatggct gattatggta
    21601 tgatgaatgg cgaaaaagtg ttagatcaag caatcgcgct cataggtgaa taaatagaac
    21661 agtataacta tcgaacaagc gatccaaaaa atagatcgct tgttttttcg tatatttttt
    21721 tagggcgaat aatatgaatc atttttgggg tagaaggctt ctgacgagag atattaaagc
    21781 aatagactgt gaggaggtaa atttaccaaa ttatgagaag atcgcagcag tggaacgttt
    21841 gggcaactgg attctttgtc atcgctgtgg aatgaggacg tccgtcattg ttggacagct
    21901 tgcagattac ggttattttt gtacacattg tctatattta gggtgatgcg acactcaaca
    21961 agatttatat ttgtttgatc agcctgaagc aaaacctcga gaagtcgtct ttttttggac
    22021 aggacagctt actgaaaaac aaacggaaat cgcaaagaga atactttgcc attcagaaaa
    22081 aagacatcat cttatttggg ctgtaacagg tgcagggaaa acagagatgc tttatcctat
    22141 tttagtggaa actttaaaag ctggaggaag agtagcgatt tgtacaccgc gagtcgatgt
    22201 ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca gaagaaaaaa tcacgttgct
    22261 ccatgggaat acagcaacgg cttatacctt tacttctttt gttatctgta cgattcatca
    22321 gctataccgt ttttatcggt cctttgattt aatagtgatc gatgagatcg atgcgtttcc
    22381 ttataacggg gatgctgggc tagctcacgc tgtgcaaaca gcaatcagat caaacggccg
    22441 attcatctac ttaagtgcca cgcctgatga gaagttacta aaggaaatca agcagacatt
    22501 tgagctccac aaacttgcct tgcgctttca tcggcgcttg cttcctgaac ccgtgcttgt
    22561 tttcatgaat aattggcgac aaaaatgtgg aaacaaaaag aaaatcaact ctttggtgcg
    22621 gatcattcag caactaatca aagaaaaccg tatcctttta ttttgtccaa gtattacgat
    22681 gatgcatcgt ttaaaaatca cactgcaaga agcttttcca gaatatgaga taacagatgt
    22741 ttcttcacaa gacgatcaac gggcaaagaa agttcagaaa atgcgagaag caatatacga
    22801 gatactgtta accacaatga tcctagaaag aggagtcaca tttgaaagaa tctctgtcat
    22861 cgttttagga gcagatcatc cagtttttac taaatcctct ttggtgcaaa tcgcaggaag
    22921 agctgatcgt aaaggtcctt atacgaacag tcgagtctat tttttctatg aagaaaaaac
    22981 ttcagctatt cgaaaagcct gtcaggaaat caaagaaatg aacgaagaag ggaagcagat
    23041 gctgtgagat gtagttgctg cggtaaagac atcattcaaa atttaacatt cagcgagatc
    23101 atcggagtgt cgaaatctag acttcctcag acatgttcag tttgccgcgc acaattccat
    23161 ttattagaag caaaaggttg tgcaggttgt caaaaaccaa tccatttgaa atacgaggag
    23221 acgaaaaaga aagcaaatac tagtttcgtt tactgcaacg actgtcaaga atggaagaag
    23281 aaatatcctg catatgattt tcaacaccgc gcctttttta cgtacgatga aaagatgcag
    23341 gcttggatca atcggtacaa gtttatggga gatatccgat tagcagggac ttttgcatat
    23401 aaatggactc agataaaaaa aagattaccc ggcatatatt tattgtccaa tacctttatc
    23461 tgaaaaaagg tggctagaaa gaggattcaa tcaagtaagc gagatgttga aagcagcaaa
    23521 aattcctttt cagatgctgc tcaaaagaga gcaacatctg cttgctcaag caaaaaacac
    23581 gcgaaaggaa agattggaga tgcctcagcc ttttgagctt gccgtacctc tttctaagat
    23641 acaacataaa aaa
//
LOCUS       DANKHL010000020        23479 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000020 DANKHL010000000
VERSION     DANKHL010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.022"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            72..2669
                     /gene="adhE"
                     /locus_tag="PW101_000567"
                     /gene_synonym="adhC"
     CDS             72..2669
                     /gene="adhE"
                     /locus_tag="PW101_000567"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDL2512994.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2898..4004
                     /locus_tag="PW101_000568"
     CDS             2898..4004
                     /locus_tag="PW101_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL2512995.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4021..5049
                     /locus_tag="PW101_000569"
     CDS             4021..5049
                     /locus_tag="PW101_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL2512996.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5055..5534
                     /locus_tag="PW101_000570"
     CDS             5055..5534
                     /locus_tag="PW101_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL2512997.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5550..6845
                     /locus_tag="PW101_000571"
     CDS             5550..6845
                     /locus_tag="PW101_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDL2512998.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6856..7680
                     /locus_tag="PW101_000572"
     CDS             6856..7680
                     /locus_tag="PW101_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL2512999.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7845..8744
                     /locus_tag="PW101_000573"
     CDS             7845..8744
                     /locus_tag="PW101_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2513000.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8870..9739)
                     /locus_tag="PW101_000574"
     CDS             complement(8870..9739)
                     /locus_tag="PW101_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HDL2513001.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9892..11352
                     /gene="rhaB"
                     /locus_tag="PW101_000575"
     CDS             9892..11352
                     /gene="rhaB"
                     /locus_tag="PW101_000575"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDL2513002.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11349..12605
                     /gene="rhaA"
                     /locus_tag="PW101_000576"
     CDS             11349..12605
                     /gene="rhaA"
                     /locus_tag="PW101_000576"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDL2513003.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12619..13464
                     /gene="rhaD"
                     /locus_tag="PW101_000577"
     CDS             12619..13464
                     /gene="rhaD"
                     /locus_tag="PW101_000577"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDL2513004.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13466..13777
                     /gene="rhaM"
                     /locus_tag="PW101_000578"
     CDS             13466..13777
                     /gene="rhaM"
                     /locus_tag="PW101_000578"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDL2513005.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13859..15010
                     /gene="fucO"
                     /locus_tag="PW101_000579"
     CDS             13859..15010
                     /gene="fucO"
                     /locus_tag="PW101_000579"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDL2513006.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            15137..15895
                     /locus_tag="PW101_000580"
     CDS             15137..15895
                     /locus_tag="PW101_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDL2513007.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            15995..16639
                     /locus_tag="PW101_000581"
     CDS             15995..16639
                     /locus_tag="PW101_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL2513008.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            16646..17653
                     /locus_tag="PW101_000582"
     CDS             16646..17653
                     /locus_tag="PW101_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL2513009.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            17669..18511
                     /gene="kduI"
                     /locus_tag="PW101_000583"
     CDS             17669..18511
                     /gene="kduI"
                     /locus_tag="PW101_000583"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDL2513010.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            18531..19334
                     /locus_tag="PW101_000584"
     CDS             18531..19334
                     /locus_tag="PW101_000584"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDL2513011.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(19583..20647)
                     /locus_tag="PW101_000585"
     CDS             complement(19583..20647)
                     /locus_tag="PW101_000585"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDL2513012.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(20644..21729)
                     /locus_tag="PW101_000586"
     CDS             complement(20644..21729)
                     /locus_tag="PW101_000586"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDL2513013.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(21742..22719)
                     /gene="mvaD"
                     /locus_tag="PW101_000587"
     CDS             complement(21742..22719)
                     /gene="mvaD"
                     /locus_tag="PW101_000587"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDL2513014.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(22712..>23479)
                     /gene="mvk"
                     /locus_tag="PW101_000588"
     CDS             complement(22712..>23479)
                     /gene="mvk"
                     /locus_tag="PW101_000588"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDL2513015.1"
                     /translation="LAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMGS
                     SAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFTK
                     GHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQA
                     KQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGGC
                     MIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
BASE COUNT         7390 a         4247 c         5232 g         6610 t
ORIGIN      
        1 gaaaaataaa tattgtatga tatacatgtg aattatttaa caaataacca tgacccatta
       61 ggaggataat gatggctaaa gtaatagaaa aagaaaaaac gaagacaatt gacgtacaag
      121 caatgattga tgatttggca gaaaaagcaa atgcagcatt gaaagaaatg gaaagctttg
      181 atcaagaaaa agtagaccat atcgtgcatg aaatggcaat ggcagctttg gatcaacata
      241 tgccattagc gaagatggct gtagaggaaa caggccgcgg tgtttacgaa gataaagcaa
      301 tcaaaaatat gtatgcgtcc gaatatattt ggcataatat caaacatgat aaaacagttg
      361 gtgtgatcaa tgaagatgtc caaaaagggt tgattgaaat tgctgaacca gtcggtgtgg
      421 tttgcggagt aactccaaca acgaatccga cttccacaac gattttcaaa tcaatgatcg
      481 cgttgaaaac aagaaatcca atcgtttttg ctttccatcc aagtgcccaa aaatcttctg
      541 cagaagcagc acgagtggta cgtgatgctg ctatcgctgc cggagctcct gaaaattgta
      601 tccaatggat cgaacatcct tcgatcgaag cgacttctat gctaatgaac catccaggta
      661 tcgcgatcgt cttagcgaca ggtggagctg gaatggtcaa gtctgcttac tctacaggaa
      721 aaccagcttt aggtgtaggt ccaggtaacg taccagctta tattgaaaaa acagcaaaaa
      781 ttaaacgtgc agtgaacgat ttgatcgttt cgaaaacatt tgataatgga atgatttgtg
      841 cttctgaaca agctgtgatc gtggataaag aaatctatgc agcagtaaaa gcagaattcc
      901 aagcacacca agtttatatc gtaaaaccag atgaattgca aaaattagaa gatgcagtga
      961 tgaacgaagg aaaatatgcg gtcaatccat caatcgttgg acattctgcg atggaaatcg
     1021 caaaattggc aggaatcagt gtaccaaaag gaacgaaaat gctgattgct gaattggaag
     1081 gcgtaggccc agattatccg ctatcaagag aaaaactttc tccagttctt gccatgatca
     1141 aagctaacaa cacggaccat gcgtttgatt tgtgtgaagg tatgttgaat cttggaggat
     1201 taggacatac agcagttatc cattcagaaa acgaagagtt gcatgtcaaa ttcggtcttc
     1261 gaatgaaagc ttgtcgtatt ttagtgaata caccatctgc agaaggcggc atcggtgata
     1321 tctataacga aatgattccg tcattgactt tgggatgcgg ttcttatggt aagaactctg
     1381 tttctcgtaa cgtctctgct gtaaacttaa tcaatgtcaa aactgtagcg aaacggagaa
     1441 ataacatgca atggttcaaa ttaccgccaa aaatcttctt tgaaaagaac tctctattgt
     1501 acttagagaa aatggaaaac gttgaacgtg tcatgatcgt ttgtgaccct ggtatggtcc
     1561 aattcggtta ctgcgataca gttcgtgaag tattggcacg acgtaaaaat gatgtgaaga
     1621 tcgaagtctt ctcagatgtt gaaccgaacc catcgacaaa taccgtatac gcaggaacga
     1681 aactgatggc agacttcaag ccagatacag taatcgctct tggaggcggt tctgcaatgg
     1741 acgcagctaa gggaatgtgg atgttctatg aacacccaga cacatctttc tttggtgcaa
     1801 aacaaaaatt cttggatatc cgtaaacgga catataagat cgacaaacca gaaaaaacac
     1861 aattcgtatg tatcccgaca acatcaggga ctggttctga agtaacgcca tttgcggtca
     1921 tcactgacag tgaaacacat gtgaaatatc cgttagcaga ttatgcattg acgccagatg
     1981 tcgcaatcgt cgatcctcaa ttcgtcatgt cagttccagc atctgtcaca gcagataccg
     2041 ggatggatgt gttaacacat gcaatcgaat cctatgtttc agtcatggct tcagattata
     2101 cacgtggatt gagcctacaa gcaatcaagt tagtctttga ctatttagaa aaatcagtaa
     2161 aaacacctga tatggaatca cgtgaaaaaa tgcacaacgc ttctacaatg gctggtatgg
     2221 cgtttgctaa cgcattccta ggtatttgtc actcagtcgc acataaaatc ggcggggaat
     2281 acgggatccc tcatggacgg acaaatgcga ttcttttacc gcatattatc cgctataatg
     2341 caaaagatcc acaaaaacat gcgatgttcc ctaaatatga ctatttccgt gcagatacag
     2401 attacgcaga tatcgcaaaa ttcttaggtc tgaaaggcga aacaacagag gaattagtcg
     2461 aagctttagc aacggctgtt tatgaattag gaaaatctgt cggcatcaac atgagtctaa
     2521 aagctcaagg agtcacacaa gaaacactag atacaaccgt agaccgtatg gctgaattag
     2581 cttacgaaga ccaatgtacg acagctaatc cgaaagaacc attgatctct gaattgaaac
     2641 agatcatcat tgatgcttac aacggataag caaaagaaag cgtagaaaaa agataagaaa
     2701 atagatgaaa aagaacttgg aaaacgttgt ttctccaagt tcttttttta atttccaagt
     2761 aactgaaagt tacttttgtt atttaacatt taggtcaatt ttagacagtt tttgttcaaa
     2821 aagcgatatt tatagtggac ggtattactg ctatattaag gatataaaat gaaagcgttt
     2881 aaaaaggtgg cgtaaagatg attattaaag aagagatttt tgtacctaaa gaattagtga
     2941 aagaaaaaat tgacctgctc gttgaaaacc tgacaacgat caaagacgat aatggtgaat
     3001 ttttgttgga ctttgatgga ctgaaagtag atgacaaaag ctggacagtg tggaattggc
     3061 cacaaggtgt cggtctatat ggtatttata aaaattacca gatgacaaaa aatcctcgcg
     3121 cttatcaagt ggtcaatgaa tggtttgagg atcgtatgga agaaggcgct ccgcccaaaa
     3181 acgtcaatac aatggcaccg ctattgacta tggcttactt gtatgaagac acgaaagatt
     3241 cacgctattt gccttattta gaacaatggg cagaatgggt catgaatgac atgcctcgga
     3301 caaatgaaga cgggttacag catgccacat atggaccgga aaataagaat cagttgtggg
     3361 acgatacatt gatgatgaca gtgcttcctt tggcaaaaat cgggaaactg ttgaatcgcc
     3421 cagaatattt agaagaagct cgttaccagt ttatgatcca tatcaaatac ttgatggata
     3481 aacgaacagg attgtggtat cacggctgga cgtttgaagg aaatcataac tatgccgaag
     3541 cattttgggc tcgtggaaac tgctggttga cgattgctat tccagagatc attgaaatct
     3601 tagaattatc gaaagaagat gcattgcgca aattattgat cgaaacactt gaagcacaag
     3661 tgcgtgcatt aaaaacttat cagtctgaaa gcggtttatg gcatacgttg ttggatgatc
     3721 cttcttctta cgtagaatct tctgcaactg caggatttgc ttacggtatt ttgaaagcgg
     3781 tccataaacg ttatttacca caagaatata aagaagtcgc ttatcgtgct atccaaggat
     3841 tgcttgaaca aatcgatgaa aaaggagaag ttcaaaacgt ttcaatcggg acaggaatgg
     3901 gggacagttt agatttttat cggaatatcg gaatcacagc aatgccttat ggacagtcgt
     3961 taacggtgct ttgtttgaca gaattgctcg tctcttattg ttaaaagaaa ggaaacccat
     4021 atgaaaaaaa catcactgct gtttgcagca agttcgctta gcctaggtct gctaggtcta
     4081 agcggctgcg gaaataattc tagtgtggca actgtcgcag atgcagatgt aaatgagata
     4141 gttaccttgc gctttgctta tgcaagcaac agccagccag taattgatgc aatgaatgaa
     4201 tttgggcgtt tagtaaaaga aaaaacagat ggttccgtac agatccagta tttcccagat
     4261 ggacaactgg gtggagaacg tgaactgatc gaattgacac agtcaggagc agtagatttc
     4321 acaaaagtaa gtgcctcagc gttggaaagt ttctctaaag attattcgat tttttcagtt
     4381 ccttatctat tcaatgatga agaacatttc tttaaggtga tggataatga agaaataatg
     4441 gagccaatct accaatcgac caaaaattta ggatttaccg gattgaccta ttatgattct
     4501 ggtcagcgca gcttttatat ggtagacggt ccgatccata cgccggaaga tttggaggga
     4561 aagaagatcc gtgtgatgca aagcgaaacc gctatcaaaa tggtagagct tttaggcggt
     4621 tcgcccgtac ctatgggaag tgatgaagtg tacacgtctc ttcaatccaa tttgatcaac
     4681 ggatcagaaa acaatgaatt tgtcttgtat acagctggac atggtggtgt ggctaaatac
     4741 tattcctatg atgagcatac acgtgtgcca gatatcatca tcatgaatga tgccatcaaa
     4801 gaacagctaa ctgatgagca ggaaaaagcc attgaagaag cagcgaaaga atcaacggtt
     4861 tttgaaattg aagcttttgc aaaagcaatt gaagaagaaa aagctttagc acaagaagaa
     4921 catggagtcc aattcaatga tgtagataat acaccgtttc gagaggcagt tgccccactt
     4981 catgatatat tcaaaaacga tcctacctac aaagaccttt atcaaaagat ccaagagcaa
     5041 ggagcgtagc tgtgatgaaa atgattttaa acaaggtatt agagatcctg ggagctgttt
     5101 tgcttgttgg gatggtcgct gttgttttat ggcaagtatt ttccagaacg gttttaggaa
     5161 atccaaatac ggtcacggaa gagcttgttc gattcggtct tgtctggttt gccatgttgt
     5221 cttccgcgta tgttgtggga caaaaagcac atttagccgt aacggtttta agtgaaaaat
     5281 taactggcaa aaaaagtaaa attttagaag ttgttgttca aagtttattt ctggtatttg
     5341 ctgcgatgat catggtgtat ggcggatgga atgctgtcac attgacaatg gggcagatct
     5401 cgccttcttt aagtattcct atgggatatg tttatctgtc agtacctgta tcgggggtac
     5461 tcatcattat ctatagcctg atcaatctaa tcgatacatt aagcaacaag ccagcaattc
     5521 aagaaaaaat ataaaaatgg aggaataaaa tggcaataga agccggatta gtgttatttt
     5581 ttgtatttgt tgtgttgctt gtattaggga tgccgattgc tataagtatt gcattctcat
     5641 cgatgatgac tttattgtta gttataccgt ttgatgtttc tgcttttagt tcagctcaaa
     5701 aaatggtggg aagtatcaat agtttttcgc tgatcgctat tccgtttttc gttctctcgg
     5761 gcattatcat gaataatgga gggatagcta agaaactagt ggattttgcg atgttgttcg
     5821 tcgggcgcgt gccgggggct ttggctcata cgaatgtgtt agggaatgct ttatttggtt
     5881 ccatctctag ctcggccatt gccgcttcta ccgcgattgg cggagtgttg atccctcagc
     5941 aagtggaaga aggatatgac cggaagtttg cgacagcggt caatatcgcg tcagctccga
     6001 caggtatggt cattcctcca agtactgctt ttatcatgta ttcacttgtt gctggtggtg
     6061 cttcgatctc tgctttgttt atgggaggtt atctcgtagg cgctttgtgg gcgctaggaa
     6121 tcatgctagt agcattcatc gttgctaaaa agaataaata ccctgttgtc gaaaaaggac
     6181 agatggctca tgctggaaaa ataataaaag aggcgattcc tagccttcta ttgattgtca
     6241 tcattatcgg tggtatcttg acggggatat ttacagcagt agaagcttca gcgatagctg
     6301 tcatttactc cctgttgatt gctatgtttt actataaaac ggtaaaacta aaagatatac
     6361 ctaatatgtt gtttcaatca gtagcaatgt cagggacgat catgttctta ttggctactt
     6421 cttccatgat gtctttcgcc atggcgttta ccggtattcc tcaggcaatt agttcgttga
     6481 ttatgggcgt aacggataat aaatttatta ttctgttatt agtcaatctt gtgttattac
     6541 tggtgggtat gtttatggat gtggcgcctg ctatcttgat ttttacacca atatttttac
     6601 caatcgtcac aagtgtaggg gttgatcctg tacattacgg gttgttttca atcatgaatc
     6661 tgtgtgtagg ctccatcacg ccgccagtag gtacaggact atatgtcggt gcaagtgtag
     6721 gaggcgtaaa agcagaggca atgctgaaac cgcttcttcc tttttatggg gttatcttag
     6781 tcgtcttgtt tttgattact tacttcccgc aaatcgtcat gtggctaccc aatatgctga
     6841 gttaggagaa tagttatgtt gaacattaca attagaggac atgatctttc cgaagtccaa
     6901 acgatagaag atcttgcaga aaaaacaaaa gaacaaggga ttcatacact gcagctcgcc
     6961 ttaggtcttt cgtttcctga aacaccttct ggcgcaaatg agatcaattc tgggatggga
     7021 aattatgtca aacgagtttt agaaaagcaa gaagtgaccg taggaatttt aagttgctat
     7081 atcaatatga ttcatccaga tttaacgata cgacaagagt tgctgacaaa gttcgagcag
     7141 tatcttcgct atgcttcttc ctttggtgcg tcgatggttg cttcggaaac tggatgtgtt
     7201 ttgccggaaa tccaatatac ggaagaaaat ttcacagatg aagcatttgc tgaagctgtg
     7261 tccgttatcc gtcgcttggt aaaagctgga gaaaaatacc aaatgatggt ggggatagaa
     7321 ccaggtttga atcatccggt gtattcttta gcaagggtag agcagctgat ccaagcagta
     7381 gattcggact atctaggaat cattttagat ccgaccaatt tgatcacaag tagtgattat
     7441 caagaacaag tccaattggt agaagaagct tttgaacgtt ttggtgaaaa gatatgtgcg
     7501 gttcatttga aagattttcg tgtcgaacag gaaaaaatcg tgccggtaaa tttgggagac
     7561 ggtgtgatcg agtacacaaa aatcaaagaa atcatcatga agaatcgtcc gtacctgtat
     7621 gtggttcttg aagaaacaaa agatgatggg attcgttatg gaagaagtct tcttgaataa
     7681 tgtaaagaag acgttggtta actaatattt agaatggagg gggcggggct agaagatgcc
     7741 acgtctcttc ttttttataa gagtatataa aagcagctct ttttgattac aaaaaacgtg
     7801 gtataataaa attgtgcata agtgaacaaa aggaggtgcg gcacatgctc caaaagaaaa
     7861 tcagcggtga acaagtcaat acgatggttt ctgatcagta tgaaatctat catgtcaaag
     7921 atgtcgtcag ccaaaataaa acgatctatc atcatcatga tttctatgag atccatgcta
     7981 cattaaaggg agaagcgact ttttatttga acggacgaca atttacgatc aaagcaggaa
     8041 gtgtgctgct catccattcc aatgatttgc atcgtatcgt ccgccaatct tcagatgtat
     8101 tcgagcgtgt ctatatcttt gtcacccctt cttttctaga aagtcgttcc acgccatggt
     8161 ctaatctatc ggcttgtttt caaccaatgg gagagaagag gagtcgtgta ttgcaaatag
     8221 aacaggaagt attgaagcaa aaattatcat ttatcgacca tcctcctgat aaaaaaattt
     8281 acggtgaaga cataaaatat gaacaagctc tggtagaata cttgattttt ctcaatcaag
     8341 ttgtacaaaa agaagagagt gcgcaagaac agaatgctgt agttcctaat gagcgtgtgg
     8401 aacaaatgtt acaatatatc tcaaaaaatt tatctcatcc gctttcttta gaggaaatgg
     8461 aaaagcagtt ttttgtcagc aaataccata tcacacgaga atttaaaaag tacactggat
     8521 ttacgtttca tcagtatgta atgaagaaaa agctgatcta tgccaagcac ttgttgcgag
     8581 aataccgcag ctctagtgct gtatatagta aaagtggttt ttcttcttat ccgcattttc
     8641 tgaaagcatt caagagagag ttcggcataa cgcctaaaga atttttgaaa cgtgatacac
     8701 agaaacagtt ggttcattat gaacattttg aagatgaaca atgagaatga tcactaaaaa
     8761 ataaccgcag tagctgctgt tgtttacata gaagcagaaa ctgcggttat tttattttta
     8821 tctatatatt tcaaaatatt ttgaaaaaat gacgtacata ttatatgaat cagctgaatt
     8881 tacgaaagtc tctcggcgat agtcctgttt ttgctttgaa caggcggcta aagtaagcaa
     8941 aattctcaat ccctatttct tcggaaatca ttcgaaccgt aaattctgta taccgaagaa
     9001 ggtctttagc gtaagaaagt tttttgctga tcaaataatc gattggtgga gaaccgataa
     9061 atttggaaaa atctttgctc agctgatatt tattcaataa gaattgttgt tctaattgat
     9121 caagtgtaat cgtttctttg aaatgttcgt ctaagtattg cttcagtttg taaacgtagg
     9181 cgggaatatc ctcttcttcg aaatctagct gatacttttg caaaatcagt tcattcagca
     9241 actcgtggat agctacagag gactggaaat cggtacgggc attcgtgcct tgttgcatgt
     9301 ggatcaattc tgtgatcaaa tggtggatct tgttttctga tggatcccct gttgaatgga
     9361 aggtgttatg gccgtctttt gtgaactcct gataaaatga ttggacgttc tgaccattca
     9421 aatggatcca atccatttcc catggttctt cgctgatcgt ttgataacgc tgatattctt
     9481 ggcagtcaat aaaaaataag tctccagctt ttagctgttg ctgattatca ttatatgtaa
     9541 gcgaaccttt gccagacaaa gtaaacttta tcaagtaaga aggtaaatgt gctcgttcag
     9601 tatagtaagg gattggtgct ttgaagtaac cgatttcttg gatataaaaa aataaattcc
     9661 tgcttctttg tgtaggcgtc aagatcactc tttttgagtc ttccgaccat gagtggttgg
     9721 ctttttccca gtaatccatg taaacactcc caacttgtca agataatgca attatatacc
     9781 aaaatagtgt gaagcaaaat ataagattct gtattatgat agcattgtta ataaatgata
     9841 acgctttatt aataaagtgc taatcaacaa taaagtaaaa gaggtggaga aatgaatcca
     9901 acatttgttg ccatcgatat cggtgcatca agcggacgac taatgaaaag tacgagggat
     9961 gcaaaaggtt ctttgatctt ggaagaagta caccggttca aaaacggctt tcggaacaaa
    10021 gacggtcacg atcgctggaa gatcgattat cttatcaaag agattttgat tggatttgaa
    10081 aaattaaagc agacagggat tgatcgcctt tttgcgggga tcgatacatg ggcagtcgat
    10141 tactgtctga ttgatgaaca aggggatcgg ataaatgatc cgattgctta tcgggacaag
    10201 cgtaccaatg gagcagtaga acagttttct gaaatctatt ctctagataa gttgtatgag
    10261 cagacaggga ttcagatcca gcctttcaat accttgttcc aattgtttgc tgaagaaaaa
    10321 gaaaatttgg caaaagcaca taaattgtta ctgatacctg attatttagg gtatgtcttt
    10381 actggtaaca tggtgacgga gaaaactaat gcatctacta tgcagctgct gaatgcaggg
    10441 acaaaagaat gggaaataga acttcttgaa aaaatcggtg tctcgaagga attatttcca
    10501 ccattgacag agccgggaac gatactagga gatctgcaaa aagaacaatt ttcaaaatat
    10561 gatttgccag atgtgacctt tatcacagta gcaagtcatg atacggcgtc agctgtatta
    10621 ggaacgccag gtcaaggaga acgttggggt tatatcagca gtgggacatg gtcgctttta
    10681 gggattgaaa caaagatcgc caatgtttct cgcaatgctt tttctgaaaa ttatacgaat
    10741 gaatggggtg cccataacac ggtgcgtttt ttgaaaaaca tcatggggat gtggttgatc
    10801 caggaagttg cccgaatgca agagtacgag tacagttatg cggaattggc agagcttgct
    10861 gcaaaagaaa aaccgctgca gcaagtgatg gaaatcaatg accctcgatt tttgaatccc
    10921 ccaaatatga tagaagagat tcagcaatac tgttttgaaa cagagcagcc agtccctgtc
    10981 tcatcgggag ctatcgctcg ttgtgtgtat gataatcttg ctctttgtta cgcacaagag
    11041 ctgatcaaag tagaacactt aactggaacg aaattggaaa agctgcacat cgtagggggc
    11101 ggatcgaata atcgtctact gaatcaattg acagcagatt atgccggaat cattgtagaa
    11161 gcaggaccta gtgaagccac agcaatcggg aatgttgcca tgcaaatgct cagtacaggt
    11221 cagttttcga atatagaaca agcaagaaat gtaatcaaac agtcgtttcc atgtgaaact
    11281 ttttacccag atgaaaataa gcaaaacacg atggaatggt acgtgaataa aatcaaagga
    11341 gtaatcgtat gaacacgatt gaaagaaatt acgaacaagc aaaagaaaaa tatgcaacta
    11401 tcggtgtaga tacagatgct gtactagaaa agatacagga catcaaaatt tctatgcatt
    11461 gctggcaagg agatgatgtc aaaggtttct tgacaccgga tggcgaattg accggtggaa
    11521 tcatggcgac agggaatttt ccaggagccg cacgaacacc tgaagagctt agacaagatc
    11581 tggagaaggc ttattcgttg ataccgggca aacacaaatt gaaccttcat gctatctatt
    11641 tggatacaga agaatctgtc gatctaaatg aaattgagcc gaaacatttt gaaaaatggg
    11701 tagagtgggc aaaaaaagaa gaaatcggtc ttgacttcaa tccgactttc ttttcgcatc
    11761 caatgatgaa agacggcttt acattggctc atccagataa agaagttcgt gaatactgga
    11821 tcgaacacgg gaaaagaagc cgcaaaatcg cggagtactt tgggaaagaa actgggcaaa
    11881 caagtatcaa taacttttgg gtacctgatg ggatgaaaga taatccgatt gatcgtttta
    11941 ccccacggga acgtttaatg gaatcactcg atgaaatttt tacagaagag ttagatgaac
    12001 ggtatgtaca agatgcagta gaaagcaaac tatttggttt aggcgctgaa gcttatacag
    12061 tcggttccca tgaattttat atgggttacg ggttgactcg tgacaaactt gtttgtctag
    12121 atgctggaca ttttcatcca acagaagtga tttccaataa attatcttca ttagctttat
    12181 tcagcaaagg aatcatgttg catgtttccc gtccagtgcg ttgggattca gaccatgtgg
    12241 ttttgatgga tgatgaattg caggaaatcg ctaaagaatt agtccgtaac gaattgttgg
    12301 aaaaaacaaa tatcggtctg gatttctttg atgcaacgat caaccgtatc gctgcttggg
    12361 tcatcggtac gagaaataca caaaaagcac tgctgaaagc tatgctggaa ccagttgagc
    12421 gattgaaaga aatggagctg gcttttgact ttacttcaag aatggcttat acggaagaat
    12481 tgaaagattt cccgtatgcc gatgtatgga attatttctg ttacaaaaat caagttccag
    12541 ttggtttgga ttggctggaa gaagtccagg aatatcaaaa agacgtgttg gaattaagaa
    12601 aataggagtg tggctatatt gaaaaaaata gatattttac aagcgcctta tgtgaaagaa
    12661 atgatcgaaa cgacgtcgaa tctttatcgc ttgggatggg atgaaagaaa tggcgggaat
    12721 atttcttatt tactaaaaga agaagagatc acaccgttct tggatcctga aaaagtgatc
    12781 cgccgcatct cgatgatttt tgatgccagc cgtttagcag gatgctattt tattgtaact
    12841 ggttcaggaa gatattttaa aaatgtagcg agcgaacctg ccgagaatct tggaatcgtt
    12901 cgagttgctg aagatggcaa aacactggaa cttttgtggg ggctggaaaa cgaatgcctt
    12961 cctacaagcg agttgccaag tcatttcatg agtcatatcg cccgtttaga agtagaccca
    13021 gataatcgga tcgttatgca ttgccatgct agtcatctat tagcgatgag ttttacgcat
    13081 gaattagacg aacgttcctt tagtcgtacg ctatggcaaa tgtgtacgga atgcttagtt
    13141 gtttttccag aaggaatttc catcattccg tggatggttc cgggaacaaa tgagattggg
    13201 gaagcaacag ctgaaaaaat gaaagaaact cgattggttt tatggccaca acatggaatc
    13261 tacgggtcag gaaaagacat ggatgaagtc tttggtttga tcgagacagc agaaaaggca
    13321 gcagaagtct atacgtacgt caaagcgcaa ggaccaatct tgcaaacaat cacggacgaa
    13381 aacctgtggc atttagcaga tgcgtttggt gtcactccaa aagcaggtta tttggaggaa
    13441 gttcatacga aagcaggtgt ctgacatggt gaaaaaagct ttttgcatga aagtttaccc
    13501 agaaaaacaa gaagaataca aaaaaagaca tgatgaactt tggccagaaa tgcgtacagc
    13561 acttcgtgaa cacggggtca tcgactattc gatctatctt gatccgagta caagtttgtt
    13621 atttggttac ttagtgatcg aagatgaaaa gttatgggaa aaaatggcaa atacatctat
    13681 caatcaaaaa tggtggaagt acatggaaga tatcatggag acaaatccag atgcctctcc
    13741 agtcagttgg gaattgatgc cagtctttga actgtagcaa caagcaactg ggcaaccggt
    13801 tgcttggggt tctttcgttt ttattgtgaa aaaaaataca aattaggagg aatagaaaat
    13861 gagtaatcga atgattttaa atgaaacatc gtattttgga aaaggagcga ttgagtcgat
    13921 cgtccctgaa tttcagcagc gaggattcgg caaagcagct gtcattactg acaaaggatt
    13981 gatcgagcac ggtatagcaa caaaagtaac agaacttcta gataaagcag caatcgctta
    14041 tgctttatac gacgggatcg tacctaatcc aacgatccaa aatgtaaaag atggagtggc
    14101 ttttgttaaa gaggcagagg cagactgttt gatcgcaatc ggtggaggtt cgccaatcga
    14161 tacggcaaaa gccattggga ttattttgac gaatccagag ttttcagacg tcattagttt
    14221 agaaggtgtt gctgatacga aaaatccatg tttacctata ttagctatac caacgacttc
    14281 tggaacagca gcagaagtaa cgatcaacta cgtcatcaca gatgaagtaa atcaccgaaa
    14341 atttgtttgt gttgatcctc atgatatccc aatcgtcgcg ttcatcgaca gcgatatgat
    14401 gatgggaatg ccgaaaaaat tggctgcatc aacaggtatg gatgctatga cgcatgctat
    14461 tgaagggttt atcacaaaag gcgcttggga aatgactgat atgctgcatt taaaagcaat
    14521 cgaaatcatc ggccattctt tggaagtttc tgtagatggc gatcaaaatg ggcgagaaaa
    14581 aatggcatta ggacagtata ttgcgggaat gggcttctca aatgtcggtt taggactggt
    14641 acacgggatg gctcacccgt taagtgcatg gtacaacatt cctcatggcg tagcgtgtgc
    14701 agcacttttg ccaactgtca tgaaatataa caaagaatat actggagaaa agtaccgtga
    14761 gatcgctctt gttctaggga ttaaaggagc agctgaaatg tctttagaag atgtaagaga
    14821 agcggcttgt ggtgagatcg atcggttgag caaagcggtt ggaatcccag aaaccatttc
    14881 tgaattaggt gtaaaagaag cagatattcc agcaattgca gaagatgctt tgcgagatgt
    14941 atgcacacca ggaaatccaa gagaaacgac agtagaagaa atcattgctt tatatcaaag
    15001 tttgatgtaa aaacgaagcg gatcttcaac attaaattgc ctgtaaagag aaatggtagt
    15061 ttatatctgg atatctcttt taaattgatg gtatttccgc tataattttt cttataaaga
    15121 aataggagaa tattatatgg aaaataaaaa gccttatgga actgtattga tcaaagcctc
    15181 taaaatacta gactatttag cggaaaacca agatgtctct ttgcagacga tcgcaaaggg
    15241 tgtgggaatg acctcttcta cagtcttgaa aatactagat actttgttga tgatcggcta
    15301 cgtaaataaa aattcagaga aaaactatcg tttaggtgca aagcttattc gctacgcgaa
    15361 taaaaacatt gaacaaatcg atttggtaga gttgacttta ccgtttttag aacagctgca
    15421 gcaaaagatc gatgagacta tccacttagg ggtattggac aacaatgaga tcttgtacgt
    15481 gaacaaacta gatccaaaac atcaaacgat ccgcatgtct tcaaaaatcg gaatcacccg
    15541 accattatac aattctgcga tgggcaaagc cgtattagca gaattcagtg aagagcagta
    15601 cactgattat cttgaaaagc atccgttgat cccttacaca gaatatacga tcaccaatgc
    15661 attacggttg aaaaaggaaa tccaaacggt aaaagaaacc cacatcgcat ttgacgatga
    15721 agaaatcgaa cgtgatattt tttgtatagg agcctcaatc gtgaaaaacg aacaaatcat
    15781 tggtgcattt agtgtaagta tgccgaaata tcgtttaaca gatgagacaa aagaacaaat
    15841 aatcagtgcc ctcaagcaaa caaaaagcga aatcgagcaa agaattcaaa aataatgaaa
    15901 aaagcgtttg caatttttct tttctgccgt ataatatggg tatagatttc caagttataa
    15961 aataaaattt ctaaatagga aataggtgga gaagatgaaa aaaattgata ttttatctcg
    16021 attgaaaaac gccggagtga ttgcggtggt acgcggaaaa agcaaagaag aagctttgaa
    16081 tgcttgccac gcaatcatca aaggtggtct aactggtatt gagttgactt ttacagtccc
    16141 tcaagcagat caagtaatca aagaactttt gtcattctac aaagaccaac cagaaatagt
    16201 gattggcgca ggcacagtat tagacgctgt cacagctcgc ttggctatct tagctggcgc
    16261 agagtatatt gttagccctt cctttgatca agaaacggca gagatgtgca atctgtatca
    16321 aattccttac ttaccaggat gtatgactat taccgagatc aaaacagcgc tgaaaagcgg
    16381 ggtagatatc gtcaaactat ttcctggaag cgcttatgga ccaagtatca tttctgcctt
    16441 caaggcgccg atgcctcaag tcaatatcat gccaactggc ggtgtgagtc tggacaatat
    16501 gaaagagtgg ttcgatgctg gtgtcgtaac agtcggagta ggtgggaacc ttttagcacc
    16561 cgcagctaca ggagattttg acaaagtaac ggaagtcgcg caacagtatg cagcaaaaat
    16621 gaaagaaatc aagaggtagc tgcttatggg aaaagtagtg acactcggag aaatcatgtt
    16681 gcgattgtcc acagagcagg gaacccgtat cgattcaagt gaacgattcg ctgcccatta
    16741 tggcggaggg gaagcaaacg tagcaatttc tttagctaat tacggacatg aagcgatttt
    16801 tgctacaaaa gtacctgata acgcattggg aaaagcagtc aaaaagcact tgcaatgtta
    16861 tggagtagat acgaaacagg tattatctgg tggatcaaga ctaggtactt attatatgga
    16921 aactggtagt ggcgaacgcg ccgcctcagt gatctatgat cgtgccggat caagttttgc
    16981 tgaaatggat catttggaat ggtcgctaga ggagttattc caaggaatag atatatttca
    17041 tgtttcagga atcaccccag ctttgtctaa aaaatggcaa aaaatgacga aacaggtgat
    17101 agaagctgca aaaaaagctg gctgcctgat cagcttcgat atcaactatc gtggaaaatt
    17161 atggacacag caagaagcca gcgaagtaat ccatcagctt ttacctttag tagatatctg
    17221 ctcagctgga aaaatggatg ctctttattt gttaggtatc gagaaagcgc cagaagcaga
    17281 agaacaacct ctaatttatt attatcaaaa aatgcaggaa aagttcccga atattcaggt
    17341 gttttattcg accaagcgta ccgttcattc agcaagtgaa aatgaattga aggggacatt
    17401 gtggatgaac catgaatatg tggaatccca agtccataga atcaccccaa tcgtggatcg
    17461 agtaggtggc ggagatgctt ttgcaggtgg cgtgctccat gggattttat ccaatatgtc
    17521 tccgcaagaa atcattgatt ttgctacggc agcttctgcc ttgaagcata cgatccatgg
    17581 ggactgcaac caatttagtc aagaagaagt cgcacaattt ttagcatgcg gctctggaaa
    17641 aatcattcga tagaaggagt agaagaaaat gcaagaaatg gaaacacgtt atacacacag
    17701 tccagaagat atccgtcatt attcaacgga acaattgaga aaagaatttt tagtagaaaa
    17761 ggtatttgaa ccagggaaaa tcagtttgac ttatacgcac aacgatcgaa tgatctttgg
    17821 tggagtgaca cctactgaaa aagaattaga aatcacatta gacaaagaac taggtgttga
    17881 ttacttctta gagcgtcgtg aactaggtgt gatcaatatc ggtggaccag gattcatcga
    17941 gatcgatggg caaaaagaag aaatgaaaaa acaagacgga tactatatcg gaaaagaaac
    18001 acgacatgtt gtattttctt ctcaagatgc tgaaaaccca gctaaattct atattagttc
    18061 tgttccagct catcataaat atccaaatgt aaaaatcagt atcgaccaag taaaaccaat
    18121 ggagactggt gaaggcctga cactgaatga acgtaaaatc taccaataca tccatccaaa
    18181 tgtatgcgaa agctgtcaat tacaaatggg ttatacgatt ttagaaccag gaagcgcttg
    18241 gaatacgatg ccttgtcata cacatgaacg tcgtatggaa gcttatgtct actttgatta
    18301 tgccaatgaa gatacacgtg tgttccacat gatgggcaaa ccagatgaaa caaaacattt
    18361 agttgtagac aatgaacaag ccattatttc gccaagctgg tcgatccatt caggtgtagg
    18421 tacaagcaat tactcattca tctgggcaat gtgcggagaa aatatcacct atacagacat
    18481 ggatatggtg caaatggatc aattaaaata agcataaggg agagaaaatg atggaattta
    18541 gtatggatat gtttcgttta gatggaaaag tagcattagt caccggtgct gtctatggta
    18601 ttggatttga gattgcacgc tctttagcta cagcaggagc aaaaatcgta tttaataact
    18661 tgaatcaaga atcagttgat gaaggtatag cgcattataa agaagcaggc atcgatgcaa
    18721 aaggttatgt ctgcgatgtg accgatgaag aagcggttca agcgatggtt caacagatca
    18781 aagaagatgt aggttcagtt gatattctag tcaacaatgc agggatcatc aaacggacac
    18841 cgatgatcga aatggatgcg gcagacttcc gccaagtaat cgacgtggat ttaaatgccc
    18901 cattcatcgt ttcaaaagca gttattccgg atatgatcga aaaaggcggc ggtaaaatca
    18961 tcaatatctg ctccatgatg agtgaattag gacgcgaaac ggtcagtgct tatgctgcgg
    19021 caaaaggcgg attaaaaatg ctgacgaaaa acatcgcttc tgaatacggt caatacaata
    19081 tccaatgtaa tggaattgga ccaggttata ttgctacacc gcaaactgct ccattacgcg
    19141 aaacacaaga aaacggtgaa cgtcatccat tcgatcaatt tatcgttgga cgtacgccag
    19201 cagcacgctg gggcgagcca atcgatttgg ctggaccatc tgtcttcttg gcttctagcg
    19261 catctgattt cgttaacggc catgtcttgt atgtggacgg aggcatactt gcctatatcg
    19321 gaaaacaacc ttaaaagagc tttagtacac tgtttagtgg agaggttgtg aaatcagcgg
    19381 attgaacgaa taccgaagga cagctgatta aacatcattt attcgaaaaa agaggctggg
    19441 acagaagcat ttaactccaa gaaataagaa ggaattcacg aaaattgctt ttcaaatttt
    19501 tgtgaatttc agcttatttc cgaaggagtt gcttttactt ccgccgtttg tacgtgttta
    19561 gagcgtgaga caaaagtgtt ctttactttt gtcccacgct cttttctgtg cgtcttccat
    19621 acttcactaa gtcgatccct cttgcttcgc accaatcctt cactggtccg gaaaagatca
    19681 gagattgttg gtgtagggcc gcggtattcg ttgcgccgac catagtataa agcagacgaa
    19741 gctcttcttg ccattgcttc atcagtataa tagtttcttc tacgccgtga ttcatcagat
    19801 gcgtcagtac ggttcctgaa gtacccacgg catttgcgcc gagacaaagg gctttaaata
    19861 tgtcataggc attgcggata cctccggagg ctaggatttc catagaagtg tctgcttcat
    19921 ttgcttcaag caatgaagag actgtcgatt gcccccagtc agctaaataa ctcagttccc
    19981 gcttggagcg acgggcgttt tcgatctggg tgaagcttgt tccgcttcga ccgctgatat
    20041 caactgtatg aactcctaaa gcagctagat cattgagcgt ttctcttgtc atgccaaaac
    20101 cgacttcttt tacgatgagg gggacgtcaa tggctgtttg agtttcttga ataagtactt
    20161 tccaattggt aaaatcacgg tctccctcag gcataaccaa ctcttgaggt gcattcaaat
    20221 gaatctgtaa ggcatctgcg tgaaacaatc ggatagcttc tttggctctt tcgacagaag
    20281 tccctgcacc gatgttggca atgattttcc cgtgaggata ttcttgtcgc ataatcgtat
    20341 aagtatcggc taaagaaggg actttcaagg cagcgctgac tgaaccagtg gcaatcatca
    20401 aatctgcttc tcttgcgacg attgcaagat cttggttgat tttttttgtt ttttcgcttc
    20461 cgccagtcat cgcattgata taaaatgggc tgcttagtgt aaacccaact gcctgtgtac
    20521 ttagatcgac atcagaaact gctatttgcg gcaaagggtt atggatgatc cgtacgaagt
    20581 caaattcgtt tttttgttta tcatgaaagg ctttagctag tgaaacatgt tcgtcttttc
    20641 gattcatcgg ttttcctttc tttgatcgct atagacatgt aacggcagtg gagtgatttc
    20701 tgctttttcc catgcactca ttaaaggaag aatgccagat ttctggtcaa caattacgat
    20761 tccgcaatcg cccccacctg caccagaaga ttttgcggct ccttcatact gttcagctaa
    20821 attacacaat ttgttcaaag caggcgtttc gatgacgacc ccagtgattg cagataaatc
    20881 atgcagtaat tgtcggtttt ttcgaatcat ctgttggatc aacgttacat tattttcttt
    20941 aaaccctttg atcatttcgt tgacacattc tgtactattt tttaagaaaa gctgataagc
    21001 caccatttta tcttctctcg aacggtgaac ttgatcgacc aaatcagaag tagaggcagg
    21061 gctacccgtc caaccaatca ataaacgtaa atcttcagga gcaatcaatg gctcaatgga
    21121 tagacctggc caatccaggg caagtaactc actgatagaa tgctgagttt cttgttcttg
    21181 gagccaagga tggtcgaagg ttgagaaagc gatccagcca ccataacagc tagcagcgat
    21241 gtcgccgcag gaaccattat cttgaactgc taaattggct agtgctgcaa tcttgaaaat
    21301 ctccaactga gataaattca acgcgtaaaa aacattcaag gctttgaccg ttgcaacggt
    21361 cacggcaccg ctagaaccca atccatattt ccggccattt gaactgtcta actcacttgt
    21421 tactttcaga tcataaaagg ataaaagaat gtttttttct tgtgcatact tttcagtcaa
    21481 gcgaatagca gcaaggatat aatgaaaagg attttcccga atatctaaaa ctaattctcc
    21541 gttgcgtctt gtccaacgta caggcatccc actatattgt gcagattgga tacttccgac
    21601 ttttcgtgca gattctacag tgactgtcac gaattgatcg actgcagcga taactgctgg
    21661 atggcctgtt tcaacaactg catattctcc ggcaatatag agtttgcctg gtgcagatac
    21721 ttcaatcata aaaatctccc tttattcaat aatcgcaatt cctggaccag catgtgcagt
    21781 aattacttgt tctttgctaa ataatttggt aaatgtttct tgtacctcag ataaatggct
    21841 gttttccact aaaactttga cattagggcc agcatccatc gtaaagtagc atggaatgcc
    21901 ttgctttctc aattgtcgca ccgcatccat cgcttttaag ctgtcaggag accagtaagt
    21961 gaatggaggc tgggcagcta aagtcgttcc atgcattttt agtccgtttc tttccatcgt
    22021 ttctccgaga agttggaaat cttttgtttt gattgcttgt ttcaattgat atagatcccc
    22081 ttcaacggaa tctaaccagc cttgataaaa actggatgtt tcgactgtcc gacgcatccc
    22141 atttctgctg gacacttctt ttttctggtc gttgatcaaa acgaaaacca ttgctaaatc
    22201 gtcttcgaaa gaatcggaag ggactggctt agcgtaagaa cttaagtcgt catggccttt
    22261 ttcccattcg acgaaaccac cgaaaatact tcggcaagct gaaccagacc cgcgtcgagc
    22321 aaatcttgaa agagagagat catctaatcc aagcttaaga gcagtattgc aagcgcctgc
    22381 taaagcagct agcccgctgg cagaggaggc taatccagct gcggtaggaa cgaaattctg
    22441 actgattact gaagctttta gtgagaggcc ggcttcttta cgaaaaaggt caagaaactg
    22501 actgactttt tttgttgctt tttcgtcttg caaggtgcca tctagataaa attcatctac
    22561 catatagcta tcagaaaaga tgacttctgt ctctgtataa aatgcatcca atgttaacga
    22621 aaggctgttg ttcattggaa ggatgagttc ttcatttttc ttaccccaat atttgattag
    22681 agcaatgttg gtatatgcgc gtgctttgcc tttaaacata ggtatgtact cctaatcctt
    22741 gtatccaagt ttcagcagca cccgcatctt caagtgcttg actgatttct tgggcttttg
    22801 tttttgtttg tgctaatgca atcatacagc caccgcgtcc accgccagtt aatttggcac
    22861 ccaatgcccc ggtatctttt gctgtttgga tcaatagatt aagaaaatca ttgctgattg
    22921 tcagcttttc cagaagtgat tgtgattcat ccattgtctg tgccaacgtt tctggtgaat
    22981 tttcaatgat tgcttgtttt gcttgcttcg tcaagtatcc taatttttgg atcatttgtc
    23041 cagtttcatg aggctgggtt tcaaagaggt gagcgacatc tttgacggct tctcttgttt
    23101 gtcctttgat tcctgtatca gcgacaatca aaaaagcatc gatgttcaaa gaaaagtagt
    23161 cgaaaggatg cccttttgta aaataaatcg gctgcaagct gctagtagcg gctgcatcga
    23221 ttccactagg attaccgtgg gcgatttttt ccgaaagctg gacattttct aatagtattt
    23281 cacgagacaa aggaaatgct aagtaatcat aaaaagcacg agtgactgct gtggcgactg
    23341 cagcgcttga tcccattcca cgttcagcag gaatcgtgct ttcaatggtc agttgcaagt
    23401 ttttttggat gtcatgctgt ttttttaagt gaatgaataa ttttttgata ttttttaatg
    23461 catgaggagc ttcggctaa
//
LOCUS       DANKHL010000021        23167 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000021 DANKHL010000000
VERSION     DANKHL010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.023"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="PW101_000589"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="PW101_000589"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDL2513016.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="PW101_000590"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="PW101_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDL2513017.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="PW101_000591"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="PW101_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDL2513018.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="PW101_000592"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="PW101_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDL2513019.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="PW101_000593"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="PW101_000593"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDL2513020.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="PW101_000594"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="PW101_000594"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDL2513021.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="PW101_000595"
     CDS             complement(8053..8523)
                     /locus_tag="PW101_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDL2513022.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="PW101_000596"
     CDS             complement(8569..8994)
                     /locus_tag="PW101_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDL2513023.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="PW101_000597"
     CDS             complement(9061..9330)
                     /locus_tag="PW101_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDL2513024.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="PW101_000598"
     CDS             complement(9341..10936)
                     /locus_tag="PW101_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2513025.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="PW101_000599"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="PW101_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDL2513026.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="PW101_000600"
     CDS             complement(14489..15049)
                     /locus_tag="PW101_000600"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDL2513027.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="PW101_000601"
     CDS             15402..16376
                     /locus_tag="PW101_000601"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL2513028.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="PW101_000602"
     CDS             16546..17946
                     /locus_tag="PW101_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDL2513029.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="PW101_000603"
     CDS             complement(18024..18746)
                     /locus_tag="PW101_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2513030.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="PW101_000604"
     CDS             complement(18825..19331)
                     /locus_tag="PW101_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL2513031.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="PW101_000605"
     CDS             19454..21253
                     /locus_tag="PW101_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDL2513032.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="PW101_000606"
     CDS             complement(21321..22895)
                     /locus_tag="PW101_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2513033.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         6354 a         5026 c         4228 g         7559 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataa
//
LOCUS       DANKHL010000022        23134 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000022 DANKHL010000000
VERSION     DANKHL010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.024"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            7..864
                     /locus_tag="PW101_000607"
     CDS             7..864
                     /locus_tag="PW101_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDL2513034.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            979..1899
                     /gene="pstC"
                     /locus_tag="PW101_000608"
     CDS             979..1899
                     /gene="pstC"
                     /locus_tag="PW101_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDL2513035.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1899..2783
                     /gene="pstA"
                     /locus_tag="PW101_000609"
     CDS             1899..2783
                     /gene="pstA"
                     /locus_tag="PW101_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDL2513036.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2798..3604
                     /gene="pstB"
                     /locus_tag="PW101_000610"
     CDS             2798..3604
                     /gene="pstB"
                     /locus_tag="PW101_000610"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL2513037.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3620..4378
                     /locus_tag="PW101_000611"
     CDS             3620..4378
                     /locus_tag="PW101_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL2513038.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4391..5068
                     /gene="phoU"
                     /locus_tag="PW101_000612"
     CDS             4391..5068
                     /gene="phoU"
                     /locus_tag="PW101_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDL2513039.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5306..5605
                     /locus_tag="PW101_000613"
     CDS             5306..5605
                     /locus_tag="PW101_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513040.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKK"
     gene            5631..6338
                     /locus_tag="PW101_000614"
     CDS             5631..6338
                     /locus_tag="PW101_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL2513041.1"
                     /translation="MSIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLL
                     NISILFVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYE
                     ELNYLTRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQV
                     GRDVIGSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVL
                     SIPISVLFFLTVRRLKR"
     gene            6335..7102
                     /locus_tag="PW101_000615"
     CDS             6335..7102
                     /locus_tag="PW101_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL2513042.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7202..8770
                     /gene="liaX"
                     /locus_tag="PW101_000616"
     CDS             7202..8770
                     /gene="liaX"
                     /locus_tag="PW101_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HDL2513043.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9067..9387
                     /locus_tag="PW101_000617"
     CDS             9067..9387
                     /locus_tag="PW101_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HDL2513044.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9387..9737
                     /locus_tag="PW101_000618"
     CDS             9387..9737
                     /locus_tag="PW101_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL2513045.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9904..10842
                     /locus_tag="PW101_000619"
     CDS             9904..10842
                     /locus_tag="PW101_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDL2513046.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10857..11687
                     /locus_tag="PW101_000620"
     CDS             10857..11687
                     /locus_tag="PW101_000620"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL2513047.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11731..12756
                     /locus_tag="PW101_000621"
     CDS             11731..12756
                     /locus_tag="PW101_000621"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL2513048.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12771..13709
                     /gene="galU"
                     /locus_tag="PW101_000622"
     CDS             12771..13709
                     /gene="galU"
                     /locus_tag="PW101_000622"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL2513049.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13834..14274
                     /locus_tag="PW101_000623"
     CDS             13834..14274
                     /locus_tag="PW101_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDL2513050.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14277..14879
                     /locus_tag="PW101_000624"
     CDS             14277..14879
                     /locus_tag="PW101_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL2513051.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14947..16053)
                     /locus_tag="PW101_000625"
     CDS             complement(14947..16053)
                     /locus_tag="PW101_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL2513052.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16263..17264
                     /gene="ccpA"
                     /locus_tag="PW101_000626"
     CDS             16263..17264
                     /gene="ccpA"
                     /locus_tag="PW101_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDL2513053.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17600..19945)
                     /locus_tag="PW101_000627"
     CDS             complement(17600..19945)
                     /locus_tag="PW101_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL2513054.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20224..21126
                     /locus_tag="PW101_000628"
     CDS             20224..21126
                     /locus_tag="PW101_000628"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDL2513055.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21339..23114
                     /locus_tag="PW101_000629"
     CDS             21339..23114
                     /locus_tag="PW101_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDL2513056.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
BASE COUNT         7361 a         4121 c         4889 g         6763 t
ORIGIN      
        1 tttctaatga aaaaacttaa attattctta ccaataatag gattagccat tcttttatca
       61 ggctgttcaa agtggattga tcgaggagaa tccattacgg ctgttggctc ttctgcttta
      121 cagcctttag tagaaacagt tgctgaaact taccagacag aaaatcctgg aaaattcatc
      181 aatgtacaag ggggcggaag cggaactggt ttgtctcaag tacagtcagg agcagtcgac
      241 atcggaaact ctgatttgtt tgcggaagaa aaatcgggga tcgatgcctc agcattagtc
      301 gatcatcgag tagcagttgt tgggatcact cctattgtga acaaggatat tggcgtaaaa
      361 gacatcagta tggaaaacct gaaaaaacta tttacaggcg agatcaaaaa ctggagtgaa
      421 gtcggtggaa aagatcagcc agtagttata ttgaaccgcg cttcaggaag cggaacccgt
      481 agcacgtttg aaaaatgggt attggacgga gaaacagctg ttcaggcaca agaacaagat
      541 tcaagcggaa tggtgcgcca gatcgttgcc gacacacccg gtgcaatcag ctatgttgct
      601 ttttcttatg tttccgatga tgtagatacg ttgaatgtta acggtgtaga accgacagat
      661 gaaaatgtga caacgaacaa atggacgatt tgggcatatg agcatatgta cacaagagat
      721 catccaaaaa aactgacaaa agaattttta gactatatct tatctgatga agtacaagat
      781 aacatcgttg gagaactagg ttatatccct gtttctcata tgaaagtcga acgtgattgg
      841 caaggaaata tcgttaaaga gtaatcttta cacaaaattt acaaacttag ctaaactttt
      901 acatgttatt aaaaccaaat tgatgttcaa tttgtacact gagtgagtat gttaaacctg
      961 attaaggggg agtcatctgt ggaagatgtg aaaaaagtat tattaacaaa atcaaaacgt
     1021 tcaaaaatgg aacagcgtgg aaaattcatc agttttttat gtatcgcgct gatcgtgttg
     1081 gttgtattat ctatttttta ttttgtagct agtaaaggat tggccacctt ttttactgat
     1141 aagatcaatg tatttgattt tctgtttggt acagattgga atccaagcca aatcggagca
     1201 aatgggaaac caatggtagg ggcattgcct atgatcacag gttcgtttat tgtgaccttt
     1261 ttatctgcta tcgttgccac gccatttgcc attggtgcag cagtattcat gacggagatc
     1321 tctcctaaaa acggaacgaa gatcttacag ccggtcatcg agctgcttgt aggtattcct
     1381 tctgtcgttt atggttttat cggtttatca gttatcgtgc cttttgtcag aagtattttt
     1441 ggcgggacag ggtttggtat cttagctggt acatttgtgt tgttcgttat gatcttgccg
     1501 actgtcacaa cgatgactgt tgatgcatta aaggcagtgc cacggcacta tcgggaagct
     1561 tcattagcgc ttggtgccac aagatggcaa acaatctaca aagtcgtgct acgagcagcc
     1621 gtgcccggga ttttaacagc agtcgttttt ggtatggctc gtgcgtttgg tgaagcctta
     1681 gctatccaaa tggtcatcgg taatgcagca ttgatgccaa caggacttac tactcctgct
     1741 tctacattga cgtccatctt aacaatggga atcggaaata caatcatggg aacagttgaa
     1801 aataatgtgt tatggtcatt agcgctgatc ttgctattga tgtcattatt ctttaatatc
     1861 gtgatccgta tgattgggaa gaaaggagag ctgaaataat gaacgcaaaa cgctcagata
     1921 aaattgctac aggtgtttta tatatagttt cggggatcat cgtattgatt ttagcagctt
     1981 tactacttta tatcttagtt cggggaatac cgcatatttc atggtccttc ttgaccgaac
     2041 cttccaaagc ctaccaagaa ggcggtggaa tcgggatcca gctatttaac tcgatttatt
     2101 tgcttttgat cacgatgatc atcagttttc ctatttcttt aggtgcgggt atctatcttt
     2161 cagaatatgc tggaaaaaac tggctaacag atgtgattcg cacgtcgatt gaaatcttga
     2221 gctcgttgcc ttctgtcgtt gtcggtttat tcggtttctt ggttttcgtt attcaattcc
     2281 agtatggttt ctcgattatt tctggtgcat tggctttgac tgtattcaac ttgcctcttt
     2341 tgactagaac agttgaggag tctttacaag cggtccatta cacacaacga gaagcaggat
     2401 tggccttagg tttgtcacgc tgggaaacag tgataaaagt ggtggttcca gaagcgacac
     2461 cagggatttt gactggtgtg atcctaagtt ctggaagaat ttttggtgaa gcagctgcat
     2521 tgatctatac ggctggacaa agtgcacctg cgttagattt tacgaactgg aatccattaa
     2581 gtgtttcaag cccgatcagc attttccgtc aagctgaaac attagctgtc catatttgga
     2641 agatcaatac agaaggcacg atgcctgatg gtgtttctgt ttcggctggc gcttctgccg
     2701 tgttgatcat tgctgtttta ctgttcaact tcggcgctcg tgcgattggc aaacgattat
     2761 accgaaaaat gacttcagcc taaggagaag aagcaaaatg aaaaaatata actggaatga
     2821 aacaaatgtc atcaccatgc caaaagagaa agaagtagca ctatatacgg atgatctaca
     2881 tgtctggtat ggcgacaatg aagcaatcaa aggagtggat ctggaatttg aaaaaaataa
     2941 gatcactgca ctgattggtc cttccggctg cggaaaatct acgtatcttc gttcgttgaa
     3001 tcgaatgaat gatgggatcg ctaatacgaa aatcactggg aaaatcatgt atcaaggcgt
     3061 agatattaat gcgcctcaag tggatgttta tgagatgcgc aaacggattg gcatggtttt
     3121 tcaacgtcct aatccgttca gcaagtcgat ttatgacaat attacatttg ctttgaaaca
     3181 gcacggcgaa aaaaacaaaa agaaattaga tgaaatcgtt gaaacaagct tgaaacaagc
     3241 tgccttgtgg gaccaagtaa aagacagctt ggacaaaagt gccttggctt tatctggcgg
     3301 gcaacagcaa cgtttatgta ttgctcgtgc gatcgcgatg aaacctgata tcttgctgct
     3361 agacgagccg gcaagtgctt tggaccctat ttcgacagga acggttgaag aaacacttgt
     3421 aaacttgaaa gaagactata cgatcatcat cgtgacgcat aacatgcaac aagcagctcg
     3481 tatcagcgac tatactgcct ttttctatct aggaaaagta atggaatatg acaaaactaa
     3541 caagatattt accagaccaa aaattcaggc aaccgaagat tatgtgtccg gtcattttgg
     3601 ttaagacgga ggcctcaaca tgacaaaaga aattatttca tctaaagatc ttcatctgta
     3661 ttatggaaag aaagaagcac taaaagggat caatctaagt ttcaatcaag gggaaatcac
     3721 tgcgatgatc ggtccttctg gttgcgggaa atccacttat ctgcgttctt tgaaccggat
     3781 gaatgacctg attccgaatg taacgatcac cggaagtgtt ttatataaag acaaagacat
     3841 ctatggtcca aaaacagatg ttgttgaact aagaaaagaa atcggcatgg tattccagca
     3901 gccaaaccca tttccttttt ctatctatga aaatgtcatc tatggattga aattaaaagg
     3961 ggaaaaagat aaaaaagtcc ttgatcaagt agttgaagaa agcctgaagg cagcgtctgt
     4021 ttgggatgat gtgaaagaca aattgcataa aagtgcttta tctttatccg gtgggcagca
     4081 gcagcgtgta tgtatcgcac gtgttttggc agttaatccg gaaatcatct tattggatga
     4141 accaacaagt gcgcttgatc ctgtttctac tgggaaaatc gaaagtatgc tgttagaatt
     4201 aagagagcgg tatacgatga tcatcgtgac ccataatatg tctcaagctt cacgtatttc
     4261 agataagacg gcattctttt tagatggtca cttgatcgaa tacaacgata cgaagaagat
     4321 attcatgaat ccagaaaaac aagagacaga agattatatt tcaggtcgat ttggataagg
     4381 aggaaacgga atgctacgta cacaatttga agaagaattg ctgaatctcc ataatcagtt
     4441 ctacgaaatg gggatgatgg tgagcagcgc cgtgcacaag tcagttcgtg catacatcaa
     4501 acacgataaa atcttagccc aagaagttat cgaaaacgat ataaatatca ataacatgga
     4561 aacacgcttg gagaagaaaa gttttgaaat gatcgcattg caacagccag ttacaactga
     4621 tttgcgtatg atcattacgg tcatgaaagc tagctcggat ttagaacgta tggccgatca
     4681 tgcggtgtct gttgctaaat ccacgattcg cgtaaaaggg cagacgagaa taccggaaat
     4741 cgaaaaagag atctccgata tgtccgatta tgtgaaaaaa atggtggaca acgtcctagt
     4801 tgcttatgtc aaaacagacg aaaaagacgc acgtacgatc gccaatatgg atcaaagagt
     4861 caatgactat ttcaatcgaa tctataatgc aacaatcaaa aatatgcagg aaaatcctga
     4921 gacagtcatc agtggaacag actacttgaa tgttgccagt tatctagaac ggatcggaga
     4981 ctatgtgaca aacatctgtg aatggatcgt ctacttggct acagggaaaa tcactgagtt
     5041 aaatacgaac cacaatgaga ttttctaaac tgaagctgtg attatgccgt ttgtttagga
     5101 aaagaggttg tgaaatcagc ggttgtgaaa aagctgttct gcatcaagta ataagaacag
     5161 tcaaacaaaa aatagctcct catatttttc gtttggctgg acttattacc gcagatgcaa
     5221 gcttttgaac accgtttatt cgggaaaggg tttgtgacaa aacatgtcac aaaccctttt
     5281 catcatttca tagaaaggat aaagaatgca aaaaaaaatt agcatcatca ctatattttt
     5341 tatttgtgtg cttacttcat gggttattca gcatcaagct gctttatatg aagtacctgt
     5401 catcgagatc gaaaaagtac gaataaaaga ggatcatcaa ttgattacag gaagactgat
     5461 gaacacggat caaaaaggag aatcgattta tttatcggtt ccttatcaaa aaaatcaaat
     5521 agaaacagca aatataaaag cgagacaaat gtatctagtc caaatgcatg gagatgaatg
     5581 gcagctagta gcaaaaaaaa aatgatggct ggcttttctt cttcacaagt atctccattg
     5641 gcgccatgtt gcttattggc ggaaaacagg gtctatttgc tttaggaagt ctcatcatca
     5701 atatcgtttt acttttactt ttgttgctca tctttactaa aacagaaggg atttctctat
     5761 taaatatatc tatcctgttc gtcgttatag ggatgattat tagcatattg gctttagatg
     5821 gatggaatag aagcagtata atcaaaattt cagctgcagt tacgagtgtt tttttagcat
     5881 tttttatttg tcttttgaca atgaatcatt ggaaagacca aggactgcgc tatgaggaac
     5941 tgaattatct gacacgaccg tatcgttctg tttttctttc tagcgtactg attggaacag
     6001 ctggaggaac actcgatacc gttatcacag tcattgcgac attagaagaa ttgactaagc
     6061 gacaaccaga aataacagcg aaacagttat ggctttcagg aaggcaagtg ggtcgagatg
     6121 tgattggctc gatgaccaat atccttttgt tcgtctatat cagtgggtca gttccatcgc
     6181 ttcttttata tttaggaaat caatggtcgt tcaaagaaac gatagaacag catttatcat
     6241 tggaattttt acgcgtcatt gctggcagct taggaatagt attatcgatc cctatctcgg
     6301 tcctcttttt tctgactgta aggaggctga aacgatgaat gtacttatta tacttgttgg
     6361 gattttagca atcagcgtgc tacttattgg cggtacgcaa ggtatgtata ttcttttagg
     6421 cttattcatc aatttaggga tctttttcct gttgttattc ggttatcatc agaagtggcc
     6481 gatactggca ttgtcggtca taggattttt attgattgct gtggttatct tattttttat
     6541 caacggttat aatctaaaaa tgcgtgcagc ttttgtgagt atactgatct ttttattttg
     6601 tttcctacta ttgcttccta tcactgattt tttagcgatc caaggtttta catcggtaga
     6661 actagaagaa ttatctggct tagataagac tttagcgatt gattttagat tacttacgag
     6721 atcgttgctt ctgattagtc ttagtggtgc tgttttagat gctagtgtag caatttctag
     6781 tgggacattc gaagtatatc aggcaaatcc gcaattatct ttttaccaat tgcgccattc
     6841 tagtttcgct attgctaaaa aaatattggc gtcaacagta atgacacttc tttttgcatt
     6901 catgggaagt tctttagcgt tgatcctctg gtttattgat ttagacattc cttttcagca
     6961 agtgatcaat gaaaaatcat ttgttctaga atatacgatg gctattctta ccacattgtc
     7021 tgcactactt gttcttcctt taacctgcgt gactacagca tatctcttta caaaaaagaa
     7081 agaacaactc aagatggagt aaaagttaca acttaagtcc tatgacattt ttactgaatc
     7141 agtgcataat atggatgtaa ggaaaagctt gcaaacaatt ttgcttaagg aggcaatttc
     7201 catgaaagaa agagaacgta ttttagattt agtgaaaaaa ggaatccttt cgactgaaga
     7261 aggcttagat ttattagaaa gcatggcaac tgaaaaagac gaaaagcaaa taaaaaaaga
     7321 agcagacaag gtaactgcta gtcataaaga aaaagatcag gcaagtcaat tgatcgataa
     7381 acttgaaaac ggcgaagaag aaatttctga gccagttgat ccaaaggaaa gagaaaggca
     7441 agatcaagaa aacttagaaa agatcttaga tgaactagca actgaagcga ataaaacttc
     7501 ggcgcgtcta gatgaagtaa atgctcaatt cgctgacaat aaggcagcac gcaatgagaa
     7561 acaggaagca ttgatgcagc tgaatacaaa agaagaatta ggcgagttga cagaagaaga
     7621 attagcacag cgtcagacgt tagaagcaga aatcaaagaa ttagaagcta gtggcgatac
     7681 actgttagaa gaacaattga aattagaagc agaattaaaa gatatccgta agaatcaatg
     7741 gtctgagaaa aaagaaacgt ttactgataa gtttgaattg ccagatgatt ggaaagatca
     7801 agcaacagat acactgaatc aagtaggcga aaaaatgtca gaagctggat cacagttagg
     7861 caaattcttg aagaaaacat tccaaactgt ttcagaaaca gtgaatgaca acatggaatg
     7921 gaaagatgtc agcttacgtg tccctgggat tgcgacaacg aaatttgagc atgaatttta
     7981 ctatgaagct cctgcagcaa gtatccttga tatcaaggca gcaaacggaa acgtgacgtt
     8041 aaaaacgtgg gacagcaacg atgtgaaagt tgaagctaag atcaaacttt acggaaaaat
     8101 gggtgctgaa ccatttgaag cattctctga acggagccag attgaggtta atgaagatca
     8161 tatctccttc caaattccaa ataaacgtgt gcgtgcagac cttgttttct acttacctaa
     8221 acgagtatac gaccacgcag ctattaaact tctaaatggc aacatcatga ttgaaacatt
     8281 ggaagctaaa gatatttata caaaatctac gaatggtaac atcatcgtgg accaattaac
     8341 tgcaacgatg ctggaagtag aaggtgtaaa cggcaatatc gatattcgaa acgggaacat
     8401 cttagattct attatcgaaa cagtaaatgg tacagtgaca tttggtgcga cagcagaaaa
     8461 cctttctgtg tctttggtaa atggtgatgt acgtcttacg attaaagaag ataatttgaa
     8521 aaaagtagaa gcaagttcag tgaacggaaa tgtcaaagtc gctttaccag atggcatcgg
     8581 tttggaagga catgctaaaa caagtcttgg aagtatcaac agccgtctgt ccaattacga
     8641 agtagttcgt gagaaaaaag aacggacaaa ccaaatgttg caattccgtc gtgtttcaga
     8701 tggtgaaatc acgcaagtgc aactttcaac gacaacagga agtatctatt taaaagatac
     8761 agataaataa agaggttgtg aaagagatgt tcagccttga gcaataagaa gcgattttgt
     8821 aaaatagctt cccctatttt acaaaattgt gccttattgc cgataggcta tctcttgaac
     8881 accgtttacc aagagattat gaacctgcca attatcgccg agtcacaagg aataatttca
     8941 gaaaaagctc gtcacgtttt atgaatatgg taatttgtga ccgagaattt tgcagaaaga
     9001 caccgattaa gaagaaggtt gtcacaacga ttttcttcat ttaaaatagt agaacagagg
     9061 tgagacatga tgaaaaaaaa gttgacaaaa tccaataaaa atgttgtttt gacagggact
     9121 ttagcaggaa taggagaata catcgggatt gatccgactg ttatccgtgt gctttatgta
     9181 ttcctgagct tagttgcttt tggaagtcca atcattttat acattttatt agctttgatt
     9241 atcccatctg cgcgttctgc acgaacaaac tatggacata acaatgcata ttatgaaaaa
     9301 aacaattatc gtgaacaaac tcgtgaacaa acaaaaaaac gcaaagaagc ggaaaaaatc
     9361 gatgatgacg aatggagtga cttttaatgt cgtactttca acgtatttta gttaatacgt
     9421 taacattcat atcattggct gttctttttc ctgcgatggt ccatgtgagc agtatttgga
     9481 cggccgttct tgccagcttc gtattgtctg tgttgaatat gtttgtcaaa ccgattttga
     9541 cgatactgtc cctgcccttt acattagtca cattgggatt gtttacgttt gttatcaatg
     9601 cagcgattct taaaatgacc tccttttttg tgggagcaga acattttagt ttttcttcat
     9661 tcggtgcagc gttgttagta gctatcttga tgtcgcttgt caatgtaatt gtcagtgaac
     9721 ataacagtag aaaataaaag aaagctgtgc cgaactatcg gcgcagtttt tttatttaga
     9781 atcaggaatg cgttttctat tacattggta attaaataaa aacgtttttt ttaattggta
     9841 gtacttttgg ctagacaatg ataaaatgat agagaacact ctaatagaaa tgaggtagca
     9901 tgaatgtcag aagttgtaaa agttagccaa ttagtgaaaa aattatcttt ggaaatcgtg
     9961 acaggcgacg agaaaagctt agaccgtgtc atcgtaactg gggacatctc acgcccagga
    10021 ctagagttga ctggttattt taattattat tcccataatc gtttacagct atttggcagt
    10081 aaagaaatca gttttgcgga acggatgatc ccagaagagc gtttgatcat catgagaaga
    10141 ttatgtagtg aagatatgcc cgcatttatt atttcaagag gattaccggc tccagaagaa
    10201 atgatccagg cagcaaatga aaatgggata gctgtactgc gttctcccat ctcaacttct
    10261 cgtttattag gagaattatc cagttattta gatggtcgcc ttgcccctcg aacaagtgtc
    10321 cacggtgttt tagtggatgt ttacggatta ggtgtactga ttcaaggtga cagtgggata
    10381 ggaaaaagtg aaacagcgtt ggagttgatc aaacgcggtc atcgattgat cgcagacgat
    10441 cgagtagatg tttatcagca agatgaatta acggtgatcg gagaaccgcc taaaatcctg
    10501 gaacatctaa ttgaaatccg aggaatcggg atcattgatg taatgaatct ttttggtgcg
    10561 agtgcggtac gtggctctat gcaagtccag ttggctgttt atctggaagc atgggcaaaa
    10621 gataagaaat acgaccgtct gggctctgaa gacacgaccg ttgaaatcgc ggaagtgggc
    10681 attccacaaa taaaaatccc tgtcaaaaca ggacgaaacg ttgcaatcat cattgaagta
    10741 gccgcgatga atttccgtgc acgtacaatg ggctttgacg caacgaaaac atttgaagaa
    10801 agattaagtc gcttgattga agaaaactca ggcaacgact agcaaggagt gacaatatgt
    10861 tagcacaaat caatcgagtt gcttttgagc ttgtagggat tcctatctac tggtatgcat
    10921 tgatcatcgt atcaggtatc attatcgcca tgtggcttag cagcagagag gctgttcggg
    10981 taggattaaa agcagatgat gtgacagatt ttatgttgtg gggattgcct atctccatta
    11041 ttggtgctcg cttatattat gttttatttg atcttcccca gtatatagct gatccaatcc
    11101 aaatattcaa tatccgttca ggaggcttag ctatttatgg cggactgatt gcaggcgcga
    11161 tcacgctgta tttttttact aaatatcatt ttatttcttt gtggactttt ttagatatcg
    11221 ctgcaccaag tgtattatta gcacaggcaa tcggtcgttg gggaaatttc atgaaccacg
    11281 aagcatatgg accagacacg acgcgaagct tcttagaaag tctgcattta ccgcaattca
    11341 tcattgataa tatgtatatc gaagggatct atcgtcaacc aacatttctt tatgaatcac
    11401 tttggagttt gattggattt attttgcttt tgtttttacg aaagaaacca caccttttga
    11461 aaaaaggcga agtcctttta tgttatctaa tgtggtatag tttcgggcgt ttctttattg
    11521 aaggattacg tacagacagt ctttactttt taggtgtgat ccgtatttct caattattat
    11581 ctgctgtgtt atttgtcggt acattgattc tatttatttg gcgcagaaaa aaaggaaatt
    11641 taccggatta tgaccgctca ttaggaaaaa atcaacaaat tatctgattt ttagagcgtt
    11701 aaaaatgaaa aaaatttcag gagtggtaaa atgaattata gagtagcggt tttaggacca
    11761 ggctcttggg gtactgcttt ggcacaagtc ctcgccgaaa acggacatga cgttcggatt
    11821 tggggacatc gtgcttcaca agtagaagaa atcaacaccc aacacacgaa tcaacgttat
    11881 ttaccagatc attatctacc cgattccatc caagcattca ccaatatgga agatgcagta
    11941 aaagacgcag atgcggtgct attcgtcatt ccaacgaaag caatacgttc tgttgcaaaa
    12001 gaactagtgc ctaaattgaa tacaaagcca gtgatcatcc atgcaagtaa aggattagaa
    12061 cagggaacac ataaaaggat ctctgacatc ttgttagagg aaatcccagc agaaaaaaga
    12121 aaagctgttg ttgttctttc tggaccaagc catgcagaag aagtagcgaa acatgacatc
    12181 actacgatca cagctgcaag tacagatgca aaagcagcaa aatatgtaca agagctgttc
    12241 atgaataatt attttcggat ctataccaat cctgatgtga tcggtgtaga gatgggggca
    12301 gctttgaaaa atattattgc tattggtgct ggcgctattc acggcttagg gtttggcgat
    12361 aatgcaaagg cagcaatcat gacgcgcgga ttagctgaga tcagccgttt aggtgtagca
    12421 atgggtgcca acccgctgac ctttatcgga ttaagcggag taggcgatct agtcgttact
    12481 tgcacaagtg ttcattctag gaactggcgc gcaggcaact tgttaggaca agggcagccc
    12541 ctagaagagg tgctagccaa tatgggaatg gtcgttgaag gtgtgaatac tactcaagca
    12601 gcgatggaac tagcccaaat aatggaaatc gaaatgccca tcactcaaac aatctatgaa
    12661 gtgctgtatg aacaaaaaga aatcaaaaca gccgcaaaag aaatcatgtt acgtgatggg
    12721 aaaaaagaaa atgaattctc attaacaatt ttttgaagga gcgaatgtat atgaaagtca
    12781 aaaaagcagt tattccagcg gcaggattag gaacaagatt tttaccagca acaaaagcta
    12841 tggcaaagga aatgttgcca attgttgata aaccaacaat ccagttcatc gttgaagaag
    12901 cgttgaaatc aggaatcgaa gacatcttga tcgttacagg aaaagctaaa cgaccaatcg
    12961 aagatcactt tgattcaaat attgaattag aaacaaacct aagtgaaaaa ggaaagaccg
    13021 aattattgaa attagtagaa gaaacgacag atgtcaactt gcatttcatc cgtcaatctc
    13081 atccaaaggg tttaggacat gccgttcttc aagcaagagc ctttattgga aatgaaccgt
    13141 ttgtcgttat gctaggcgat gatatcatgg aagatgaagt accattgaca cagcaattaa
    13201 tcgatgatta cgaacgtact catgcttcta cgatcgccgt tatgcaagtt cctcatgatg
    13261 aaacttcaaa atacggaatt atcaatccag gagaagtatt ggaagatggg ttatacaatg
    13321 tcaaaaactt tgttgaaaaa ccagatccaa gcaaagcacc aagtgattta gctattattg
    13381 gacgttactt attgactcca gaaatttttg atgtattgtc cgagcaacaa ccaggagccg
    13441 gaaatgaaat ccaactaact gacgcaatcg atacattgaa taaaacacaa cgcgtatttg
    13501 cccgtaaatt cacaggtaaa cgttacgatg tcggtgacaa atttggtttt atgaaaacaa
    13561 gtattgaata tggtctaaca catccagaag taggtgcacc tttacgtgag tatatcctgt
    13621 ctttaggtaa aaaattagaa gcagaaggaa gcaagatcga tcataatcca aaaggaataa
    13681 cagaaatcaa agaagaaaag aaaaaataac ccttttttct aacgagcttc agctgctaat
    13741 tgaataaaaa gtagttgaag ctcgttttta tttgtttaaa aaacacgaaa atgctatcct
    13801 tatagaaaag agagacaaga agggagtttg atcatgtcag gaggagaaat tgcaggattg
    13861 atcgcagcaa tcgcctttgc gatacttgtc gtttttatcg ttcgtgtgtt gttgcaagtt
    13921 tctaaaactt tagaaaaagt agatcagact gtagcagaag caaatacgac gatcgaaatc
    13981 gtcacaaaag acgtagatct tttgtctaga caagtagaag gcttgctggt caaaagtaat
    14041 gaactgctct tggatatcaa tggaaaagtc gcaacgatcg atccattgtt cacagcagtt
    14101 gctgatttaa gtgaaagtgt atcagaactt aatcattcta gtaaaaacat cgcgacgaaa
    14161 gtcggcggaa tcggtaaaac gacagctaaa gcgacggttg ccggaaaagt cggtgggaca
    14221 gcgatgaaat tttttaaaaa caaaaaacat actgaaacga caggaggaaa ataaaaatgg
    14281 ctaaaaaagg tggattttta ttaggtgcag tagtcggagg aacagccgca gcaatcgccg
    14341 cgttattgtt agcaccaaaa tctggtaaag aacttcggga tgaattagct gcccaagcgg
    14401 atgatttgat ggatatggct tcagattaca cagatatggc gaaagaaaaa tcgactgagt
    14461 taacaggtat tgcaaaagaa aaagcgcaag atctttctca acaagcaggt gatctagcag
    14521 gtagtgtcaa acaaaaagta agtgaatcag cagatgaagc aggaagtgaa tctgacgata
    14581 tcttgttaaa tcttaaagaa caatcagctg acttatctaa tcgtttcaaa aaaacggcag
    14641 aagatatgaa aaaacctgca gatgaattag gaagtattgc taaagatact tcagaagata
    14701 tcatgattga tgtaaaagac gctgctggta aagcaggaga tactgtcaat gaaggaatgt
    14761 cagaagcaaa accagttgca gatgacacaa aagaaatgat gaaaatggca aaagaagatg
    14821 cgaaagaagc taaaaaagaa ataaaagata caatgacaga ttcatctaca aatcactaaa
    14881 atcactaaaa tcacaaattt atagaataaa aaggtgggag cggactagct ctcacctttt
    14941 ttactcttat ttttctaaaa ctagtaattc ttttggtgta gtcgtgaatg gcacacagcc
    15001 atcgcttgtc acatatacac agtcttcgat acgtacccct actttttcag gaatatagat
    15061 tcctggttca agagagaagc acatcccttc ttcgattact aagtcatttc cttctacaag
    15121 ggaagggaat tcgtggacgg ttgttccgat cccgtgacct aaacggtgat tgaaatattc
    15181 gccgtagcca tagctgttga tcacgtttcg tgcgatttga tcaagttcac ctgctgtcac
    15241 acctggacgg acggcttctt gtgcggcaag ctgtgcttca agagtgatgt tgtatatttt
    15301 ttcttggaag tcggttggtt tttgataagc aattgtacga gtagcatcac tgcaatagcc
    15361 attccaaaca acgccaagat cgaataacac aagttcattc ggtgaaatct tcgtgtttcc
    15421 tggtgtacca tgagggcttg cagcatttgc tcctgcaagg actaatgtgt cgaaagacat
    15481 ttgtgaaaca ccttgttttt tcaattggta ttcgatttct gcaatgatcg cttgttcagt
    15541 cacgccttct ttcactgctt taaagccaat ctcaaatgca acatcggccc atttaccagc
    15601 ttcgatcagt ttgtcttttt cctctagagt tttgatcagt tgtagacgtt ggatgatgga
    15661 agctagatca gtgtaagaat ccgttcccgt caagatatca gagatagcat cgaagcgctc
    15721 gacagtcaag gcgttctttt caatccctac attgccaggc tgaccatagc gagcacgaag
    15781 ttcttttccg atgatctccc aagggttttc gctgtctaaa tagccacgga catcatacgc
    15841 ccaactactg ttttgagcat cttcaacttc caaagcagga gtgaaaagga aaggatcttg
    15901 ttcaagaggg ataaaaagag ctagtacacg ctcatgagga tcactttcgt atccggaaaa
    15961 gtaagcgatg tggccagggt cgctgatata agccaattcg acttggtgtt gtgccatcca
    16021 ttgacgtagt tcattgattt ttacgttatt catttggcaa cctactttct aaattaattt
    16081 tgtattcaat aattgtacca ctgatttatt aaaagaccaa cgttctaaaa actgaaaaaa
    16141 gtaaaaattt tcatgaaaac agagataaaa cggttgcaat tcttgagatt atttgatatt
    16201 ctattttaga aatgtaagac gtattttcag aaaacatata aaatatgaat aggagaacac
    16261 aaatggaaaa gcaaacaatc actatctatg atgtagcgag agaggcaaat gtttcgatgg
    16321 ctactgtttc acgtgtcgta aatggcaatc caaacgtcaa accagcaaca agaaaaaaag
    16381 tcttggaagt tatcgatcgc ttggattatc gtccaaacgc agttgcgcgt ggcttggcaa
    16441 gtaagaaaac aacaacagtt ggggtaatca tccctgatgt cagcaatatg ttctttgctt
    16501 ctttagcacg tggtatcgat gatgtggcaa caatgtacaa atacaatatt atcttagcaa
    16561 attcagatgg taacgaccaa aaagaagtca atgtattgaa taacttactg gctaagcaag
    16621 tggacggcgt gatcttcatg ggtcaccata tcacagacga aatccgtgga gaattttcac
    16681 gttcaaaaac tcctgttgta ttagctggct ctattgatcc agatgaacaa gtaggtagcg
    16741 taaacattga ttatgtatca gcaacaaaag atgcagtaaa caaattggca aaaagcggca
    16801 ataaaaaaat cgcttttgtc agtggtgcgt tgatcgatcc tatcaatgga caaaatcgtc
    16861 tgaaaggcta taaagaagca ttaagcgaaa ataacctacc atatagcgaa ggattgatct
    16921 ttgaagcaca atacaacttc aaagatggtc tatctctagt cgatcggatc cataatagcg
    16981 gtgcaacagc tgtctatgtt tcagatgatg aattagccat cggtattttg gatggtttat
    17041 tagaccgtgg cgtgaaagta ccggaagaat tcgaaatcat cacaagtaac aattcactac
    17101 tgacagaagt agctcgtcct cgtttaacaa gtatcacaca accattatac gatttagggg
    17161 cggtatctat gcgcctattg acgaaaatga tgaataaaga agaagtcgaa gaaaaaacga
    17221 ttatcttgcc ttatggaatc gaagaaaaag gatctacaaa ataataaaag gacaaccatt
    17281 cagaggttgt gagcctgccg tttaactctg cgttacaagg agcaatttca aaaaacagca
    17341 tttcatgttt gatgaatatt gtgactcgtg attcggagtt ggcaggagaa caccgtttat
    17401 tcgggaaaag gggttatgac aaaattttgt ttaatccttt ttttttgtct taaaaaagag
    17461 agaaagtggt tgaaattaca tttatcgtat ataaaacaat ccgtgtatag atgctgcatt
    17521 atgttcacca ccatcaaaaa aataagggac tgtgacaata ctattgtcac agtcccttat
    17581 tactgtgtat tctattaaat caatcatctt tctcatctga ttctttggtt gcttgattcg
    17641 ctcttgcata agctgaagcg gtgttccagt aatctgtata gttttcatcg gtaccgccaa
    17701 cgccaaattt aaaggcgctt ttcttcggtc catttttcgc ccatagcgat tcaactgttt
    17761 ttcctggtgt ttggatggat tttttattga cagtaacttt tccaggtgtt tgaccagtaa
    17821 atccagatac ttttttcttc atcacatcag atgataattc gaatttctca tcagtgccaa
    17881 aaatgtcagg ttttgcttgg taagcttgat tgatcagatt ggcaagataa ttagccgaac
    17941 gtatccttgc tttgctgtcc attccagtat tgtcatcgtg ccctgcccag ctactgatgg
    18001 taatagacgg agtagagaca attagccatg aatctcggta ttcattggta gaaccagttt
    18061 ttccgaccca atctgctttt cctagatttc cgtttaagct tgagatggca tctttgaatg
    18121 gtgtagtgat ttttgcattg atcacactgc gcatcatgtc attcatgatc gatgccgttg
    18181 cttccgaata gatccggaca gagttgcttt cgtgtttata aattacattt cctgcgttat
    18241 ctgtgatgga atcgatgata tagccttctt catagacgcc gccgtttgct aaagcctgaa
    18301 atccattggt ctgttgaagc gtcgtgacat ctactgtacc taaaggagca gattcaacgc
    18361 cccaattgtc atttgccggg tagttcatct ttgccaagta gtgctcgtaa gcatattgtt
    18421 tcgatccacc ggaattcaac acatcttgat ataagtgata agccgggata ttgttggacc
    18481 attctagtga ctcacggacg gtttggaagg tgttcgatcc ttcgttagta gcattgacga
    18541 ttttctcgcc agcattttct ccttcttgcc aagttgttgc ataatctgat acacgggatt
    18601 ctgatccgat aagccccatg tcgattgcag gtccataaac aagaactggt ttgattgatg
    18661 agccagcttg acgttctgta tcgaaggcat ggttattttg attctgactg taattccgtc
    18721 cgccaacgaa tccgtagatg cggccagttc tgttatccat caatacattt ccggtttcta
    18781 cttgactgcc agcaccatca tctaacatgt atccataatt ttctacgcca ttttgcaatg
    18841 cagcatagac atctttatcg attgtcgaat ggattgtata tcctttattt tggatcgttt
    18901 gctgtgcctt ttgataataa gcgtcactga ccgatgaatc agacatatca gcgttgtctt
    18961 tttcagccaa ttgttgtgca atgattcttg ttgcttcatt catcacagta taatacaaga
    19021 attctctgtc agtctgttct gcgatttgtt gtggtagaaa gtcttttgta aggtcatagg
    19081 cttttgcttc ttcgtattgt tctttcgtaa tttggccttc acgatacata ctgaataaaa
    19141 caaaatcttt tctggcaagg ccggcagaaa gatcttcttt tagcgcacct gtgttcgtgt
    19201 atggactata cgtgatcggg ctttggggta atccagcgat ataagcagct tgcggcagcg
    19261 tcacatcttt tgcatctaca ccaaagattc ctcttgcagc ttcctggact ccagcaatgt
    19321 tttgtccctt attattacga ccaaatggtg agacattcaa atacgtagca atgatctcat
    19381 cttttgaaaa gtatttttct acttgtgcag aaagaatgat ctcgtttgct ttacgtttga
    19441 atgttgtttc atctgtaagg atctgctgtt tgactaattg ctgtgtcaaa gttgatccac
    19501 cagaagagcc aataccagta gcttcagata ctaatgctcg taaaacagct ttaggaacgt
    19561 aaccttggtg tttatcgaag tattcatcct ctgtgctgat aattgcagtt ttcaataaag
    19621 gtgaaatttt atcagatgaa atagtcgtgc gcatcagatc tgtctggatt tttgatatct
    19681 ctgtattatc tgcataaaca atctttgacg tttctgtaat gtttcctagt tctgtctgca
    19741 gtgctttttt agtcggcaat tgtgtgtctt cgacgagata ggcaaaatag ccagcgccta
    19801 ttccaatacc tagagctgcg aacatcaaaa gaagaacagt aataaaaaca gtcaatgatt
    19861 gaaaaactct aaaaataatc ttaggaacaa accatttttc tttttttctt ctattctgtt
    19921 tttttcgagt atttttgcgt ggcaaaaggg tcacctcaac taatggtttt atttcgctaa
    19981 ttatagcaaa taatagaaca aaaaaacaga caaggctgtt tttggcagga atttcttttt
    20041 taaaagagtt aaaacgtgag aaaatgatct tttttataaa agagttctta agcttttctt
    20101 tagagaaaat ttcagttact atggagaaca gggcaaagaa aaatcaatca ttttctttgt
    20161 atatcatctt accaatatat aggaattgtt agtaacaatt gcctttcaag gaggaatcca
    20221 tccatgttat taggctcaca cgtcagcatg agcggaaaga aaatgctgtt aggagcagca
    20281 gaagaagcag ccagttatgg atcttcgact tttatgatct atacaggcgc ccctcaaaac
    20341 actagaagaa aagccatcga agagatgaat attgatgccg gaaaagcgta catggcagaa
    20401 aacggtctaa caaatatcgt cgttcatgcc ccttatatca ttaatttggg gaatacgatc
    20461 aaaccagaaa tgtttccttt tgccgtccaa tttctgagag aagagattgt tcgtgcggaa
    20521 gctttaggcg ctactcaaat tacacttcac ccaggtgctc atgtaggggc aggaacagaa
    20581 gccggaattg ctagaattat tgaaggcttg aatgaagttt tgacaaaaga acagacggca
    20641 caaatcgctt tggagacaat ggcaggtaaa ggtacggaaa tcggtcgaac atttgaggaa
    20701 ttagcgcaaa tcatcgatgg agtaaaattg aatgaaaaat tatccgttac ttttgacacc
    20761 tgtcatacga atgatgccgg ttataatatt aaagaagact tttcaggtgt aatggaagag
    20821 tttgataaga tcatcgggtt agatcggctt aaagtactcc atatcaatga ttctaaaaat
    20881 ccgcaaggtt cgcataaaga ccggcacgcc aatattggtt ttggaacaat cggattcgac
    20941 acgctgaata agatcgtcca tgatccaatg tttgacaata tttcgaaaat cttggaaact
    21001 ccctatgtcg gcaaagataa aaagaaagct tacgcaccat atggaaaaga aatcgccatg
    21061 ttcaaagcac aaatattcga tccagaagtg tttcctgagt tattagcaga agaagtgata
    21121 ctctaagatt ttctatctga cagcagcaaa aaaggaccag gcacattgcc ttgtcctttt
    21181 ttgtttaaga aacaaaggag tgctttttgc agtctttttc atcatgcatc ttctcccccc
    21241 tctttttaaa tttagacaaa cagggtatac tggtagaaaa gtaaaaaaac gaaaagtttc
    21301 acaagcaagt ggcaaaacgt gattggaagg acgaaaagat gctgaacact ttaaaaccct
    21361 tgatcaacca actaaaaaag aaacatacat actttattct tggcatagcc ctattattta
    21421 caattattcg tttgtattta tatagtaaag caatctatgg aacagacatg tcggcaacct
    21481 atgatgacca gctgctgatt cgctatgctc aaaatatttt ggacggtaaa tggctagggg
    21541 agtatacgac tacaacactt tccaaaggaa tctcctacag cttattttta gtacttgcac
    21601 ataaactgca tctttcttat gcagttttgt tcggcatgtt cagtattttt gccagtatat
    21661 tggttgcact cgcgtttaaa ccgatcgtga agaacaagta tcttctttta agtttctatt
    21721 tttatttttt gttttctccg attagtttta cacatgaata ttccactcga acgtatcgta
    21781 atgccctcgt gattccggct gttgagatca ttatcgcttg tttactagct atttattacc
    21841 gtaaatatag ttccagcaaa aaaatgctgc cgtggttttt agggctagct gtttcttttc
    21901 ctttcttttg gttcattcgc gaagattcca tctggcttct tccttttact gtagtcgcgt
    21961 taggcatcag tttcgtccag atcttgctag gacaagactt ttctttcaaa gaagcaaaga
    22021 agttaaaaga acgaccattt ctcctttcga aacaagaatg gctgaaactt tcattgtttt
    22081 tactccctct agtcggtttc tccatcgtct atcagtggat cgaatataaa aacgagacaa
    22141 catatagtat tgccgccatc aatgaccgtt cgagtggcga atttggacgt ttgatgaaac
    22201 agctgatccg aatagatgac gaaacgaact taaataaaga aaacagtgag atatgggtct
    22261 cgcatgctgc attaaataaa gcattagacg catctcctac atttcatgct gtttctactc
    22321 gtattgacga attgtatacg actcatgcgt ggacccaagg tggaaaagta aaagaattac
    22381 caggagatat cattttttgg gcattgcgag atgtggtaag cgaatctggt tattaccaaa
    22441 acaatgcaaa agaaaccaat gatttttgga aaaaagtcaa tcaagaatta gaagaagcct
    22501 atcaaaaggg aactatcaaa aagaaaaaag aattctatct gatgggatca ggggatggaa
    22561 agacgatgaa tgatctgcca atcgtatttg cttttctgaa atcaggatat gcctatgcat
    22621 ggcgatacaa agatttccat caagggggga attatagttt cggtacgcaa gaacaagtcg
    22681 aacaggccca agaattattg aatcttccac taatggataa ttggatcgat agcagtgatc
    22741 cgagaacacc tgatttccag ttgacaaaaa cagcaaaagt cgcgaatgtc atgataaaaa
    22801 tttaccaagt tctggccatt cccgtgctga ttgcttcaat agcagggata ttacttatga
    22861 tactaggaac cgcatttgca aaagctgatc gtgcctatta tagttcctct ttgattgtag
    22921 tcaccggatt aatactgaca caattggttt ttcttttcgg cgtctcgtgg ttttgttctt
    22981 atgcgccaga aaaaaaggat tattttttaa gtgtctacac tggcgcaggc gtgccattaa
    23041 cgcaattcat cgtagtaaca agcatgctag ttttcagtaa attgaatctg aaaaaagctt
    23101 tatcttccaa ataaaaacat actaagatta gagg
//
LOCUS       DANKHL010000023        23065 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000023 DANKHL010000000
VERSION     DANKHL010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.025"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            191..2662
                     /locus_tag="PW101_000630"
     CDS             191..2662
                     /locus_tag="PW101_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2513057.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2765..4501)
                     /locus_tag="PW101_000631"
     CDS             complement(2765..4501)
                     /locus_tag="PW101_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDL2513058.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4498..5202)
                     /locus_tag="PW101_000632"
     CDS             complement(4498..5202)
                     /locus_tag="PW101_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2513059.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5333..5836
                     /locus_tag="PW101_000633"
     CDS             5333..5836
                     /locus_tag="PW101_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDL2513060.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5796..6134
                     /locus_tag="PW101_000634"
     CDS             5796..6134
                     /locus_tag="PW101_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDL2513061.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6155..7573
                     /gene="ffh"
                     /locus_tag="PW101_000635"
     CDS             6155..7573
                     /gene="ffh"
                     /locus_tag="PW101_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDL2513062.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7685..8263
                     /locus_tag="PW101_000636"
     CDS             7685..8263
                     /locus_tag="PW101_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDL2513063.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8267..8788
                     /locus_tag="PW101_000637"
     CDS             8267..8788
                     /locus_tag="PW101_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2513064.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8867..9421)
                     /locus_tag="PW101_000638"
     CDS             complement(8867..9421)
                     /locus_tag="PW101_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL2513065.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9674..9949
                     /gene="rpsP"
                     /locus_tag="PW101_000639"
     CDS             9674..9949
                     /gene="rpsP"
                     /locus_tag="PW101_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDL2513066.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9961..10212
                     /locus_tag="PW101_000640"
     CDS             9961..10212
                     /locus_tag="PW101_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDL2513067.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10337..11128
                     /locus_tag="PW101_000641"
     CDS             10337..11128
                     /locus_tag="PW101_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513068.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11298..11819
                     /gene="rimM"
                     /locus_tag="PW101_000642"
     CDS             11298..11819
                     /gene="rimM"
                     /locus_tag="PW101_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDL2513069.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11809..12570
                     /gene="trmD"
                     /locus_tag="PW101_000643"
     CDS             11809..12570
                     /gene="trmD"
                     /locus_tag="PW101_000643"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDL2513070.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12706..13053
                     /gene="rplS"
                     /locus_tag="PW101_000644"
     CDS             12706..13053
                     /gene="rplS"
                     /locus_tag="PW101_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDL2513071.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13426..14088
                     /locus_tag="PW101_000645"
     CDS             13426..14088
                     /locus_tag="PW101_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513072.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14167..17394
                     /gene="fss3"
                     /locus_tag="PW101_000646"
     CDS             14167..17394
                     /gene="fss3"
                     /locus_tag="PW101_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HDL2513073.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            17508..17822
                     /locus_tag="PW101_000647"
     CDS             17508..17822
                     /locus_tag="PW101_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2513074.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            17835..18209
                     /locus_tag="PW101_000648"
     CDS             17835..18209
                     /locus_tag="PW101_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2513075.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            18219..18563
                     /locus_tag="PW101_000649"
     CDS             18219..18563
                     /locus_tag="PW101_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL2513076.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            18634..19986
                     /locus_tag="PW101_000650"
     CDS             18634..19986
                     /locus_tag="PW101_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL2513077.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            20046..21188
                     /locus_tag="PW101_000651"
     CDS             20046..21188
                     /locus_tag="PW101_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDL2513078.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            21213..21467
                     /locus_tag="PW101_000652"
     CDS             21213..21467
                     /locus_tag="PW101_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513079.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            21723..22907
                     /locus_tag="PW101_000653"
     CDS             21723..22907
                     /locus_tag="PW101_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL2513080.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            22904..23041
                     /locus_tag="PW101_000654"
     CDS             22904..23041
                     /locus_tag="PW101_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDL2513081.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         7667 a         4102 c         4962 g         6334 t
ORIGIN      
        1 aaagtgttag tagaaaatcg ccttcgattt tccaccaaca caaaatatta tagaaccctt
       61 tttttttgct atatgacagg aatgaaaggt gtttgcaagc ttaatgtgag gaacactttc
      121 catatctgtt ctatactgga taggagaagt ttggaaatat ccagaaaacg aaatcaatcg
      181 aggtagccac atgaagaata aatggataaa tcgaagtaaa aaaaagaaag taacacaaag
      241 atcatcagat aaagtcatcc gtctttttcc atcgtataaa gaaggattaa cggcagatca
      301 agtcaaggaa cgaatagaaa aaggtgcagc aaataattca gtcgatccca cattcaaaac
      361 aaatcagcaa atcgtattgg aaaacatatt tacgtatttc aatttgatct ttcttatctt
      421 agccattttg ctttgtttag tagaatccta taagaacttg acttttttac cagttatcat
      481 agccaataca ggaatcgcca tttttcagga aattcgatcc aaaaagatct tggacgaact
      541 aaatgtttta aatacagcaa aagtcaatgt catacgagac gggatagaac aaacaataga
      601 tatagaagac cttgtaatag atgacattgt tattttagag actggatatc aaattccagc
      661 agatgcagag gtcgtggaag ggaaactgca agttaatgag gctttattga caggcgaagc
      721 agatgaaata accaaagaaa taggtgatcc attaatgtcc ggcagtttca ttgtttccgg
      781 aaaagcttac gcccgattag acaaagttgg agcagattcg tatatttccc agctgacagt
      841 caaagccaag acaatgggag aaggagaaca gtcggagatg attgcttctc ttaatcaatt
      901 gatcaaatgg attgggatca tcattattcc tatcggtatc ctgctttttt ctcaaagtta
      961 cttttttaat ggaaacacga tcaaagaaag tgtcgttgca atggaagcag cattgatcgg
     1021 tatgatacct gaaggactgt acttgctgac gacgattgca ttagcattaa gtgcgactaa
     1081 actagcaaag caaagagttc ttttacacaa tatgaaaagc attgagacat tggcaagggt
     1141 aaatgtcctt tgtgtagata aaacaggaac gatcacagaa aataaaatgt ctgttcaaaa
     1201 aatgatcgtc tctaaaaaac aagaaataat cagtcagtca gatcaattgg aagcatgcat
     1261 tgctgactat gcaaaagcga tggcaggaga caatgcaaca atggaagctg ttaaagcata
     1321 ttttacaaaa accactgatc aatcctatca ccatgttatt cctttttctt ctgtccaaaa
     1381 attcagcagt gtagcgttgg cagacaaggt gtatgtttta ggagcgcccg aaatggtttt
     1441 gcgcgatcaa ttgccggaat atgcggaaga gtttgtttct ttcgctgaaa aaggctatcg
     1501 cgtgttggtc tttggtgtgt acgatgggat tcttgaagaa ccagtattga cagaagcagt
     1561 cacaccatta ggctatattc tgattgctaa tcctatcaga aaagaagcaa gagccacatt
     1621 tgattacttc aaaaaacagc aagtagccat caaagtgatt tctggagata atccgctcac
     1681 cgtatccaat gtagctgtcc aagcagggat ttcacatgct gatcagtacg ttgatgcatc
     1741 taaattatca gaagaagaat atgaagcagc tgtcgaaaaa tatacagttt ttggacgcgt
     1801 caagccagaa caaaagaaaa tcttcgttca attgataaag cgtaacaata cagtagctat
     1861 gacaggtgat ggcgtgaatg atattctagc catgaaagaa gcagactgca gtatagcgat
     1921 ggcttccgga aatgaagccg ccatgcaggc ctcacaagtg gtcttacttg attctgactt
     1981 ttcacgcatg ccagaagttg ttgcagaagg acggcgcgtt gtgaacaata ttgagcgatc
     2041 agccagcttg tttttagtga agaatatttt ctcctttttg ctctcactgt tttcagtgat
     2101 ctttgctctg acctatcctt tagaaccatc acaaatcaca ttgattagtt tgtttactat
     2161 cggacttcca tctttcttgc ttgctctaga agaaaacaaa aagcgaatca gagggaaatt
     2221 catcatgaat gtgatggaaa aagctgtgcc aggtggacta acagatatga tcgtagtcgg
     2281 tgcgttggtg atctgcgggg tgactttgaa tctaaataaa acagatgttt ctacagcatc
     2341 taccatgctt ttgattgcag tagggttcct cgttctctat aaaatctgtt caccgctgaa
     2401 taaattcaga agtcagatca ttcttttctg tgctagcggt aacttttttt cggtcatctt
     2461 tttgcataag ctgttttcaa tcaccggtat ctcagctgta tcacttttgc tgttgagcat
     2521 tctatttttc tcggcagatt cgatttttag gcatttgacc acattggtag aatatttgtt
     2581 tgaacgtaaa aatgaaaaag agcaaaagcc gtttagtcgc gtagctaaaa aatttctatc
     2641 gatatttaaa cgagaaaatt gacgaaaagt atctcaaaaa agagtaaaaa agaacaacca
     2701 ctcgtgtatc tttcgaagca ggatgagtca tccaacttta aaacgcacga gcagttgctc
     2761 ttttttacat tttcggaatg gtaacggtga aagtcgaacc tgttccaaga tgactgtcta
     2821 cagtaatcgt accattaagg agttgcacgt agtcttttac aatcgctaac cctaatccgg
     2881 tcccgcctga atgtcggctt cgcgccttgt ctacgcggta aaaacgctca aagatccgtt
     2941 cctgatcctc aatgtcgata cctataccaa agtcttggac agaaaacgat agatcatctg
     3001 ttgcctgata gcggataata atctcgctgt cgctttttga atattggaca gcattttcaa
     3061 ttagattttt gataattgga taaaggatat ctgttttagt agtaaatggt tcgttctttc
     3121 caatcaatcg aatcgtcaac cgtttctttt cgatgattgg ctgataattt tgaacgattt
     3181 gctggaaata aggttctgta taaattgtct ggatctcgta agaattctcg ccatcttttg
     3241 acagctggat gatttcccga atcagtttat ctaaccggat cgcatctttt tgcatgatct
     3301 caagaaaaga agtcagagtt tgcgggtcat cttttgcacc atccagtaaa gtctcggtaa
     3361 aaccaatcag tgaagtcaca ggagttttta attcgtgaga gacgttgcca acgaaatcct
     3421 tttgtagctt ttctaaacgt cgcactttcg ttaagtcata cgccacccct agaatctgtc
     3481 cggtaccttc tgaattagta aagtagcgca aactcaagtc taatgtctgc cgaccttctg
     3541 taagggtgat ctcctgatgg atcagcggat gctcaggagt cacttgatga atcagctgaa
     3601 tgagttttgg ctcttgaatg acttctgtgt accgctgttc aggttgatag cttaccacgt
     3661 ttaaatgtag ctgcatcttt ggattaagca gtagtaaacg agaatcagca tcatcaatca
     3721 ggaatacgcc aatcatcagt tcgttcagta acgtatataa ttgctcttct gtggtagtac
     3781 acgcccgata catcttactc atttgttcgc tcaacgcatt aatcgtctga tacaattctt
     3841 cccattgatc gggtgtttgc atgattactt ccgcctgatt aggattttgc accatttttt
     3901 ttagtacggg cagcacggtt ttcaatggct ggtttttttg atagatcaga taatagatca
     3961 ttcctgcaag aacaaggaag aaagacagaa agaagaaaaa gacccaacgg cgaaaactct
     4021 cagttctcgg taaaaaaccg cttgtcggtt ctgcgatacg taaaatggct tctaactggc
     4081 cacttttttt gactggtaat gcgacatata aaagatcctc attcaaagta gtacttttgc
     4141 gcaaagcgct acctaggttc ccaccattca atacggcttt gatttcggga cgggagtttc
     4201 gctgttcatt caatgtttca ttgtttgtgt caaataagat ctttccagta gcatccatta
     4261 aagtaatccg ttcgttcgac tgatggacga attcttccaa agcagttttg ttttgcgctg
     4321 cttggaggtt ttcgacatct aattgccgga tcagcagttg tgcttttttt gtcaggtagc
     4381 tttcttgctg ttccacgatc tgctgttgaa aataatggga aatcaattgc cagccaccaa
     4441 aaaacaaact aactaaaaga ataagaaaga agagacgttc ataaattttt ttccatttca
     4501 tcgtttgggt tcctgaaact tatagccaaa tcctctaata gtcaccaagt actttgggtg
     4561 ttttggatct cgctcgattt tttctcgcaa atgactaacg tgtacatcta cgattcggct
     4621 ttgtcctgcg aagtcaaaat tccaaatgcg gtctagcaat gtgtctcggt cgatcacacg
     4681 gtcttttcgt ttcatgaaat aaacaagtaa ttcaaattct ttcggtgtta gttcaatcag
     4741 atggtcgtct acggtcactt gataattggt caaatcagca gtgatttgtc caatcgatag
     4801 atattcaggc tcagcttcat cttttgattg cttacgaggt tcaattcgtc tgaatattgc
     4861 tttcatacgg gctaatactt cgcgaggact aaatggtttg gtcaaatagt cgtcagcacc
     4921 tatttctagg ccaatgatcc gatccacttg gtcatcttta gccgtcagca tcaaaatcgg
     4981 tgtttcattt ttttcttgac gcagccgctg agtgatctcc atgccatcca tcgttggaag
     5041 catcacatct aaaatgatga aatcaaatga ttgttctaat gctaattcta atccttcgcc
     5101 tccatctgtg gcactggtta ccttgtagcc ttctttttcc aaattgaacg tcaagagcgt
     5161 tacaatagaa ggttcatcat caacaactag tatttttttc attcaaatct cctttaaata
     5221 ggtttcattc tgtttatatt ttagcataga atcagtacaa agggctattt tacaaaagaa
     5281 ataaatggta taataggaag gcactttgct gaatagaaag gaagagcagt caatgattgg
     5341 aatcagtgca tgtttaggcg gtctggcctg tcgctatgat ggacagaaaa aagaagtacc
     5401 agaattgatg gaactagttg aaactggaga agctgtcatg gtctgtccag aagtgatcgg
     5461 cggattacca attcctagag atcccgctga aatcattggt ggaaatggat tcgatgtgtg
     5521 gaaagatcaa gcaaaagtat ggacgacaag cggcgaagat gtaacggaag cgtacaaaaa
     5581 tggcgcgatc aaagcctatc aaaaattgca ggaaaaacac attgatctag tcatcatgaa
     5641 ggaaaaaagc ccttcatgcg gaacccattc gatttatgac ggaagttttt ccggaataaa
     5701 aaaaaatggt gccggagtgg ctactgctta ttttattcag caaaaaatga ctgtgctctc
     5761 tgatgaagaa tggctgaaaa ctagaggtga gcagaatgga aatcgaaaag accaatcgaa
     5821 tgaacgcgct ctttgaattt tattcgacct tattgacaga aaaacaaatg aactacatgg
     5881 aattatatta tgcagatgat ttttcattag gtgagatcgc ggaagaatac gaagtaagcc
     5941 gacaagcagt atatgacaat atcaagcgga caagcaagat cttggaggat tatgaaaaaa
     6001 aactccatct tttttctaat tatattgttc gagaacaggt attggaaaat atgactgcat
     6061 atattacaga taagtacccg gaagataaaa aactacttga atatgtacag caaattcaag
     6121 caatcgaaga ataaataaaa ggaatgaaga aaatatggca tttgaaagct taactgaacg
     6181 tctccagcag gcgatgggaa aaataagaaa aaaaggaaaa gtatcagaag ctgacgtaaa
     6241 agaaatgatg cgggaaatcc gccttgcctt actagaagcc gatgttaact tgcaagtagt
     6301 caaagatttt actaagcgcg tacgcgaacg tgcggtaggg gcagaagtgc tggaaagttt
     6361 atcacctgca caacaaatcg taaaaatcgt tgatgaagaa ttgacgatca cacttggttc
     6421 tgaaacagca gagttaaata aatcaccaaa aataccaact gttatcatga tggctggtct
     6481 tcaaggggca ggtaaaacga cctttactgg taaattagcc aattatttaa agaaaaatga
     6541 aaatgctcgt ccgctcctaa tcgcagggga cgtatatcga ccagctgcga tcgaccaatt
     6601 gaaagtatta ggtcaacagc tagacgttcc tgtatttgat atgggaactg aagtcagccc
     6661 ggtagagatc gtccgtcaag ggatggagtt agcaaaagaa aagaaaaacg actatgtatt
     6721 gatcgatacg gcaggacgtc tgcatatcga tgaaacattg atggatgagt tgaaacaaat
     6781 caaagaattg acgcaaccaa atgagatttt gcttgttgtc gatgccatga ctggtcaaga
     6841 tgccgtcaac gtagctgata gcttcaacca gcaattaggt attacagggg tcgtcatcac
     6901 taaacttgat ggggatactc gaggcggggc agcactatcg atccgctcag tgacaggcgc
     6961 accaatcaaa ttcatcggtt ctggtgaaaa attgactgac ttagaagtat tccacccaga
     7021 tcgtatggct agccgtatcc ttggtatggg ggatatgctg acattgattg aaaaagcgca
     7081 gcaagactac gacgagaaaa aagcggaaga acttgcacag aaaatgcggg aaaattcatt
     7141 cgatttcaat gactttattg aacagcttga tcaagtgatg ggtatgggcc cactggaaga
     7201 tctaatcaaa atgatcccag gaatgaatca agtccctgga atcgaaaacg tcaaggtaga
     7261 tccaaaagat gttgaacgca aaaaagcgat ggtttattct atgacgccag cagaaagagc
     7321 aaatcctgat ctgttgaacc caagccgtcg tcgccgaatt gctgccggtt ctggaaacag
     7381 tgttgttgaa gtgaatcgaa tgatcaaaca attcaaggaa tcaagaaaaa tgatgcagca
     7441 aatgtccaaa ggggatatga acatcccagg tatggaccaa atgtttggtt caggcgtcaa
     7501 aggaaaacta ggcaaaatgg cgatgaatcg aatgatcaag aaaaataaga agaaaaagaa
     7561 aaaacggaaa taagatgaaa aaaacagctt cggtacagac tgaaaatgtc tactgccgga
     7621 gcttttctat cagcaatttt tattctttac taatacaatt actctaaacg aagaggtggc
     7681 acagatgaga tgtccgtgga gtgaacaaac agaaagtatg cggcaatatc atgatactgt
     7741 ctggggtgtt cctgaatatg atgaccaaaa gctgttccga aaattgatgc ttgatattaa
     7801 tcaagccgga ttgagctggc agacgatttt aaataaaagt gaagcattcg atgaagcata
     7861 tgatcgattc gatatcaaaa aagtcgcaaa ttatggagaa acaaaaatca gtgaactaat
     7921 ggaaaacaaa ggaattattc gtaaccgaag aaaaatcgaa gcagccgtcc aaaacgccaa
     7981 agcagtcttg aaaattcaag aagaaatcgg cagtttttca gagtatcttt ggtcgttttc
     8041 taatggaaaa atcgttgatt cttcttatca ttcacaagaa gaagtcccaa caacaagtcc
     8101 tctttcagat gaattagcaa aagatctaaa gaaaagagga tttaaattta tcggaagcac
     8161 aacaatctat gcttttttag aagctgtcgg gatcatcaat gatcaccttg acagttgttt
     8221 ccgcaaagaa gaggtcgtac gtatcggcag aaagggcgaa taaagcatgc gaaaaatcat
     8281 attttatggt gctataagtt tagatggcta tttggcaacg aaaacggatg atcttcaatg
     8341 gctttttgac actccaacag gtaagcaaac tacttatgaa gctttttatg aaacaatcga
     8401 tacgaccatc atgggcagaa aaacttatca ggaagcaaaa aaatatttgg agaccgaagc
     8461 aatttatcca gagaaaaaga atttcgtttt ttccacccac gcacaattac aattgccaga
     8521 tgctgaggtc gtaaacgaag atcctgttgc ttttctaaaa aatctacaaa tgacagaagg
     8581 aaaagatatc tgggttgtgg gtggcggtcg attgatcaaa ccgctattgg aacatcaaat
     8641 gattgatgaa tggtatatcc agattacccc tgtattatta ggggacggga ttcctttatt
     8701 ccaaaaagga acatatgaag cgcgttttga aatgctagac acgacacgat tcggtgaatt
     8761 tatcgaattg cattatgtac gaaagtagaa gtaaataaaa taaaaaaggc aactatatgc
     8821 agaaatgtcc tgtacatagt tgcctttttt attaccgtta tgctttttac tctgttaatt
     8881 tgttagaaaa atctagatac gcatctaaaa tcgtttggaa gaaacttaat gttccttcaa
     8941 cgatattacc attttcatca attagattcg caacgtttcc gatatatgct tccggttgct
     9001 gcaaggttgg cacattcaaa aagacaagtg attggcgtag atggtggttt gcgccaaagc
     9061 cgccgatccc acctggtgat gcagtaacga ctaaacctgg tttatgatcc caaacactgt
     9121 gtccataagg tcttgagcct acatctaaag catttttcaa tactgctggc acagaacggt
     9181 tgtattcagg agtaacgaaa acgacaccgt ctagtcccct cacttcctca cgaaaacgag
     9241 tccattcagc aggagcctgt tctggagttt cgagatcctc attgtaaaat ggcagatcat
     9301 cgatttttac aaatacactt tcgtaccctt caggaagcaa tttcgcaaaa gcttctgcta
     9361 cttttttgtt gtaagaatct tttcttaaac taccaataaa aaatccaatt ttttttgtca
     9421 tacgattttt cctccttcaa actatctata ataatcttac aaaagataag taactggagc
     9481 aaataaaaac acttactgac aaaagctaca tagaagatag attacttatt tttaacaaaa
     9541 aagaagtcta aaatcttcta ttttctaaaa aaaatcgaat ttaatcaagc tgtaaagaaa
     9601 aaaagcttta cagggtaaaa aaaaactgtt acactacatc ttgtgaataa aacaatggag
     9661 gtggaaataa ataatggcag ttaaaatccg tttaaaacgt atgggttcta aaaagagtcc
     9721 gttttaccgt atcgtcgtag ctgattcacg ttctcctcgt gatggacgtt tcatcgaaac
     9781 tgtaggtact tacaatcctt tgaaagaccc tgcagaagta gttttaaaag aagacttagt
     9841 tcttgactgg ttatcaaaag gtgcacaacc ttctgacact gtacgtaaca tcctttcaaa
     9901 agaaggcgtt atgcaaaaac accacgaagc taaattctca aagaaataag gtgaccggat
     9961 atgaaagatt taagcgactt agtcttaaca atcgttcgtc cgctagtcac ttatcctgat
    10021 caagtcaagt tagacgtcgt agagtcgaaa gacttttacg agtacaatct aacagtggct
    10081 cctgaagata ttggccgtat cattggaaaa caagggcgtg ttgctaaagc aatccgcaca
    10141 atcgtttacg gtgttcggat caatgcaccg aaaaaagtgc gtttgaatat catcgatgat
    10201 aaggaaaagt aaataaacgt gctgtactcg aattttcgag tgcagctttt ttgttttttt
    10261 tattaattga agaaatgatt atttggcaaa acaagcaaga aagcgtatcc ttataggagg
    10321 agggagtgaa cagccaatga gaaagaaaat gttatttatt ccatttgctc tattgttaag
    10381 cgcttgtagt tcggttactc aagaatcaga caatacacat ctatcaagca gtatatctga
    10441 aactgttacc agtccagaaa aaagtagtac caaaacggct tccaccactg aacaaacaat
    10501 ggctacctca aataatgaga aaaagactgc tttagaccag ttgaaagaac aacagccaaa
    10561 tgttcccatg cctctagatg taccagtctc ttcaggctat ctcaatatcg cagcgacaca
    10621 tacaaagcaa ggttattcta ttttatatta cagaacagat cgtcccctcg ggttgaatgc
    10681 tgacgaattg aatcaagaaa cgcctattgc aacctatcta tatcaatatg ggtttgcttc
    10741 tagtcaagaa acaatccaag ttttgcagcc atttgagatc gacacaaatg gtcagcaagt
    10801 ggacttaggt tctcgaatca cagggtatca gcaaggtgca gcaggttcaa gttttcttga
    10861 atggcaagaa ggaaactggc gtattcgaat ccgtgggaat aatatagaag gccaagatcc
    10921 cttactatta gctaaagaaa tcgtagctta tttggaagaa aacagtctac cagcacctga
    10981 acaatttggt aaaattactg ttgatatggg tgatacgacg aatcgagccg tagaagtaag
    11041 ctggcaagaa ccgaaaaatg cctatacgat cactcatcaa gatcctatgt ctgcgttgaa
    11101 gatggcagtt tctatgaaga ggttgtgaaa tcagcgcgct gatctgagcc catcatttaa
    11161 ctccaagtca caggaagtaa tcaaaaatag cacgccgcat ttttgcataa ttttggctta
    11221 ataccttaaa agaaaactag aattgcctct tcacacatgc taaaataaaa cagacaaaaa
    11281 agaaatgggg atataagttg actgaatact tgaatgtagg aaaaatcgta aacacacaag
    11341 gtatcaaagg cgaagtccgt gttatctcaa caacggattt cccagaagaa cgttacaaaa
    11401 aaggaacggt gctaacacta ttccaagacg gaaaagcacc tgtggaatta acagtcaaaa
    11461 gtcatcgcaa acataaaaat tttgacttat tgagttttga aggacatcct tctatcaatg
    11521 atgtggaaaa atatcgggat ggcatattaa aagtgtcaaa agacaactct gtagatctag
    11581 ctgaaaatga attctattac catgagatca tcggtctgca tgtattagaa gatggaactg
    11641 agttaggtaa agtaaaagaa atcttgtcac caggtgccaa tgatgtttgg gtcgttcaac
    11701 gtccgaaaaa agctgatata ttgcttccat atatcgcttc tgtcgtcaaa gaagtcgatt
    11761 tagaagctgg agtggtccgt gtagaaattc cagaaggact gattgacgat gaagattgat
    11821 gtattgactt tatttccaag aatgtttgaa ggtcctatgg gggaatccat catcggtaag
    11881 gctgtagata aaggattatt agatatcaat atctctaact tccgcgatta ttcagataac
    11941 aaacatcaga cagtagatga ttatccatac ggcggtggag caggtatgct gctcaaagtc
    12001 cagcccatct atgataacat ccaagccatt gaaaaagctg caccggatgt caaaaaaaga
    12061 gtaattcttc ttgacccagc tggtaaacga ttcgatcaga aaatggcaga agaattttca
    12121 caagaagaac acttagtatt catttgtggt cattatgagg gatatgatga acgtatccgc
    12181 acattagtga cagatgaagt ttccttagga gattatgtcc ttactggggg agaattaggt
    12241 gcgatggtca tgatcgatgc aacagttcgg cttttgccag atgtgttagg gaatcagact
    12301 tcagctcaaa cggattctta ttcaaccggt ttattggaac atccccaata tactcgcccg
    12361 gcagaatata aagggatgaa agtaccagaa gtattgacaa atggaaatca taaattgatt
    12421 gaagaatggc aattaaaaga atcactaaga aggacctatc aacgtcggcc tgatcttttg
    12481 gaaacacttg aatggacacc tcaaatgacg aaatttttag aagaaatcaa gaaagaagaa
    12541 caagaaaaaa cagcagatga agccaagtga ctttttctaa aatcttcttt acagtaggtt
    12601 gataatatgt tagtatttta tggtgagtgc aaaagcactt aactattacg atattccgct
    12661 gtgagaaaag ctcataagaa tatttggaat aaggagaaga aaaccatgaa tccattaatc
    12721 gaagaattaa caaaagaaca attacgttct gacatcccag cgttccgccc tggtgacact
    12781 gtgcgtgttc acgcaaaagt tgttgaaggt actcgtgaac gtatccagtt atttgaaggt
    12841 gttgttatca aacgccgcgg tgctggaatc agcgaaactt atacagtacg taaagtttct
    12901 aacggagtgg gcgttgaacg tacattccca ttgcatacac cacgtgttgc aaaaatcgaa
    12961 gtagttcgct acggtaaagt acgtcgtgca aaattgtact acctacgtgc attacacgga
    13021 aaagctgctc gtatcaaaga aatcagaaga taaacacgga aaacttcctt ggaaatctga
    13081 aatttgtgaa aagtccttgt atatcaaggg ctttttcttt tttctctatc acagttttaa
    13141 gccatttttg gctgttttgg accatttttc aatcatattc ccttaaaaat tgtcctagat
    13201 tgtccgacat tgactatcag tgtcgtcaaa atagggcaaa atttagtagt cagcttgacg
    13261 acaaatataa tacgaaaaat accaaaacat ttaccgaatt tagcaacttt tgaacccaag
    13321 tttctcaaag ggtcagctat tagcaaaaaa gacaaaacat gtacttttct actaaaaagt
    13381 tgatgtgata ggcttctatc taaataacta ttaggagttt ttatcgtgaa gcaaaatgat
    13441 ggacgtatca tttggaaaaa tcaaagtgag ttaaaactga ttttaacaat taatgaattc
    13501 atcgaaaaac acggaattac atctagccgt caataccaga aaaagctatc agaaaatcca
    13561 aattctgcac caagtatgtg gttcattaac aaaaaatatg gatcatggga gaatttattg
    13621 attagtatag gacgagaaaa tactgggtat ggaaaatggg ctagaatgtc agaacaagaa
    13681 ttattggaaa tagtagaagc ctttattaag tgcgagaaga taacctctca acgaatgtac
    13741 gaacaaaaat cagtagggaa gaatattcct tcacttagta ccattaaaaa gatgttagga
    13801 gatatacgtc cactttttaa agaaaaaaat gatggatctc gatttactga tttcgaactg
    13861 ctgttagaac taaaaaatga aataatccga ttaaaactac aggatgattt atcaatgaca
    13921 aagtttcgaa aactagttca atccccaaaa ctaccatcag ttgatacaat tatgaaaaga
    13981 acgaacaaaa attgggagga attgatggca gagattggat ttgattatcg aagaatcaaa
    14041 atttataagc agagaaacaa cctatccaag acaaagaaaa ctaaatagta tcactctaaa
    14101 tcagacgttc aaaattaaaa tttggacgtc ttttttatta cccaaaaacg aaaggagatt
    14161 tattttatga aaaagccaag atttaagaat tggcgtttgc tcgcaacact gagtctatta
    14221 tgccaaacaa ttggtggatc gcttggccca acgattgcct ttgctgatga aattactcat
    14281 ccacaaacgg taactgtgga attggatttg gctcaccagt atgctgtcga aggtacattt
    14341 agcgatggtc gtcccatgtc agaagttacc gttccgcact atgcagttta caatggtgtg
    14401 aagcaagata ttttctgtat tgagccaggg gtgccgattt acaatgagtt cactcctggt
    14461 tatgagaaaa atccattgcc tgatatgtcg gagaaagcga aattagtttc cgttttatgg
    14521 aaggaagctg gtacggatgt ggatacacat attgtggccc agaagatgat ttggcaagaa
    14581 gttaatggct acacgcttca ctcaattaaa cgttctgatg gtagtgcagt aaatatcgca
    14641 gccattgagt caaaaatcaa tcaagccatt gcggattatc aaaagaaacc aagcttccat
    14701 aactccacag tgaaaaccgt gttaggtcaa tcgactactg tgacagatac gaacggcttg
    14761 aatttatcgg agtttgatga agttgtcgag aatatcgcaa atattgatta tcgtgtgaac
    14821 ggcaatcaac tagtgattac tccaaatgcc aattcaaaag agagtggtga attaacccta
    14881 aagaagtcag ctaatacagg aacaccggta gcttataaaa tggctggtca acaaactttg
    14941 atggcggggg caattgataa gcccaatacc tacacaatca aaattgatgt ggaaactgag
    15001 ggttctttga agattaaaaa agtcgataaa gaaacaggaa atgttgtacc gggaaccgtt
    15061 ttccatttag actttggaaa aacattacct gcaaaagacg tgaccaccga taaagaaggc
    15121 attgcgacat tggatgagat tcctcatggc gcgaaagtaa ccattactga aaaatcagtg
    15181 ccagctcctt atacgattga cactacacca atcaccgcaa ccattaaatc tggtgagact
    15241 atttctgtga cttcaaaaaa tactcgcgaa aaaggtcaaa tcattttgaa taaaatgggg
    15301 gtggaaacag gaactgatct ttggaacgac aaatactcat tagccggaaa tacgtttgcc
    15361 attcgcaaag acagtccaac cggtgaaatt gtccaagaaa tgaccacgga tgaaaaaggt
    15421 catgcggaaa caccaaaaga gattgccaat gcgttggaat tgggaaccta ttatgtgacc
    15481 gaaaccaaag ctagtaatgg ctttgtgaat acctttaaac cagtaaaagt cgaattgaac
    15541 tatgtcaatc aaaccgtggc tcttgttacc agtaacgtaa aagggcaaaa ccaagaaatc
    15601 actggtgaaa ccactttgac aaaagaagac aaagataccg gtaatgaaac tcaaggtaaa
    15661 gctgagttta aaggagctga atatactctc tttactgcaa aagatggtca agcggtcaaa
    15721 tggagtgagg cttttaaacc agagttagtg aagggaacga aagcttctga tgaaacggtg
    15781 actttgactt tagatgaaaa gaatcaagtt gctgttaaac acttagcgat taatgagtat
    15841 tactggcaag aaaccaaagc acctgaagga tataccttgg atgaaacaaa gtaccctgtt
    15901 tccatcaaaa aggtggatga taacgaaaaa aatgccgtga ttacccgaga tgttacggca
    15961 aaagaacaag tcattcgctt tggctttgat ttctttaaat ttgctggatc agctgctggg
    16021 actgccgaaa ctggcttcaa tgatctgacc tttaaagtat cgccattgga aggaaccagt
    16081 gagattacag gtgccgaaga tgaagcaatt acagcttata acgagcaact aggttttgat
    16141 ggctatggca agtttgaaaa tcttccttat ggggattatt tacttgaaga agtagaggct
    16201 ccagaaggat ttcaaaaaat tacgccatta gaaatccgtt ctacttttaa agaaaataaa
    16261 gcggattaca ctaagagtga atacattttt acgattaccg aaaaaggaca aaaacaacca
    16321 attaaaacgg tgaccgttcc ttacgagaaa ctgacgaata aagcattttc tgttagcttg
    16381 aatcgtttga tgctctatga tttacctgaa gaagaagata gcttaacttc tcttgggact
    16441 tggaaagacg gaggcaaaga attgacagac cttgattcta ccgagctagt tgataaattg
    16501 agctataact tgcatgaaat caaagaagac tggtatgtgg tagcgcaagc cattgatgtg
    16561 gaagcgacaa aagctgccca agaaaaagat gaaaaagcca aaccagtggt aattgccgaa
    16621 acaaccgcaa cgttggcgaa taaagagaaa acaggaactt ggaaaatttt gcataaatta
    16681 accgcagaac aagttttgga taaaaccatc gtcttattca actatgtgta tgaaaacaag
    16741 aaaccctatg aagcaggtga tgatccagta gcgaaagatg ctagcttgaa caatcaagcc
    16801 caaactgtca attgtacggt ggaacgccat gtttccatcc aaacaaaagc ccacctagaa
    16861 gatggttcgc aaacctttac tcatggggat gtggtggata tgttcgatga tgtgtcgatt
    16921 acccatgatg tactggatgg ctcaaaagaa gcttttgaaa caattctgta tgcattactt
    16981 ccggataata cgaccaaaga aatttggaaa tctggcaaaa ttgattatga agtgaatgac
    17041 aaagaattca ctaaaaccgt acttgcggaa aaagtggaca caagtaaata tccagaaggt
    17101 acaagtttta ccttcaaaga aattaactat ggcaaagacg gaaacatcaa tgggaaacac
    17161 aatgaggatc taaaagaaaa aactcaaact ttaacaccta aaaaagttcc gacaacacca
    17221 agtgcaccag aacaaccgga aataccaact gttccaagtg actctcaaga atctagtcct
    17281 acagtaaaga cattcccaca aacaggggag aaaaattcta atgttctgct gtttattggg
    17341 tttaccttga tctttgcgac tgctgggtat tatttctgga atcgccgaaa ctaaggtgat
    17401 gtgatgaaac aactaaatag gaaaaaccaa cgtgcgcgac cgcctcctaa aaaaagtggt
    17461 cgcgctattt ttgtatccaa aactaaaaat gaaaagagga aaagaagatg gaattgaaat
    17521 ttgttgtgcc agatatggcc gaaacctttg gaaagataag gtatgccggc gaaggcgaag
    17581 ttttaactga aggatatggg cggaatacta cagtaattgg tcgtagttat catttgtatt
    17641 ccagtaagca acgagccgat gatattgaag tggtggtagc tgcggaagcc ggcgaaaagg
    17701 attttgacca agatcaacca ctaaaagccg tgaatcccca tttggttgcc aaaggctatg
    17761 agattgagaa tcgtgggttt accgactacg tgttgtatgt cgatgattta gtgaaagcat
    17821 aggaggaaaa gaagatgaga ttaacagaag gtattgttgt agattcagga ctaacgtttg
    17881 ggaaattacg attttccgca ttacgccgag aagtacggaa gcaaaatgag gatggaacgg
    17941 ttagtaacga ggtaaaagaa cggacttata atttgaaatc ctctgcgcaa ggtcgaatga
    18001 tccaagttag tatcccagca aatgtaccct tacgcgagtt tgcctatgac gcagaggtag
    18061 agttggtcaa tccaatcatg gatacggtcg ccaattatgt tttccgagaa ggaactactg
    18121 tcaattggtt cattaaggcc gatgatttag tcttgaaacg acaaccagat cagggaaatt
    18181 ctacaaacca aaatgaagga aagaaatagg tgaagcgtat ggaaattaat gttgaacata
    18241 ttttagattg tctcgatcaa tatggaaaag gtgagctaac ggaggaacaa ctcactaaag
    18301 cattaaccta tgatgaaaaa atgtttctga ttatgcatca agggttactg gaagtaggta
    18361 acgatgtcga agaagatttt gatgtactga attggttagg ggaagaagag tccttcttta
    18421 tggttatcga agtaaatgaa agtctatgtc gagaagccga aaccgtattg gaagaaattg
    18481 gtgtggaaat gccggatgcc attgaagttt ttttaaagca actagtggaa acgaaacaac
    18541 tccctatagc agtaaatgac tagcataact gcttgaaatt ggatcaatga aatcctatga
    18601 agcttttttc gattggaggt ggaaaccata aaaatgtaca aagggcatcg tatacgggca
    18661 ggagatcagc acttggttta tcactttgtt cttggctggc tactcgctct gttcatcggt
    18721 tggatgagcg tcttttattt tcaagagctt agacaatttg atatttctaa gctatcactt
    18781 tctactatag aaatcgttcg ttctataaaa gatttaatct atttgctagg aagtctcgtt
    18841 ctttcaggaa gcacgatgtt gctttatata cacttttttc aggatcattg gcgcagtttg
    18901 tggcatcgac aaaagctagc ccggatgatt ctagaaaatc attggtacga agtaaaacaa
    18961 actcagagtg aaggattttt caaagacctc aatagtagtc gaaccaaaga gacaatcagt
    19021 tacttcccca aaatctatta ccgtatgaag gatggtttac tttctattcg cgttcaaatt
    19081 tcactgggaa aatatcaaga tcagctttta aagctggaaa agaagttaga aagtggtcta
    19141 tattgtgaac tagtagaaaa ggaactcaaa gactcctatg tggagtacac tttactgtat
    19201 gatataattg ccaatcgaat tgggatagac gaagtagtgg cagaaagtgg ggctttgcga
    19261 ttgatgaaaa atcaagtatg ggcttatgat tctttacctc atatgttaat cgctggcggc
    19321 acaggtggtg ggaagactta ctttttactc actatcatcg aggctttatt aaaatctgat
    19381 gccgagcttt ttgttcttga tcccaaaaat gcggacttag ccgatttagg tacggtaatg
    19441 cctcatgtct attcccaaaa ggaggaaatt tctgcttgtg tggaagattt ctatgagcgc
    19501 atgatgactc gtagcaaggc aatgaaagaa atgcccaact acaaaacagg agagaattat
    19561 gcgtttcttg gacttccacc aaacttttta atctttgatg aatacgtggt ttatatggaa
    19621 atgttaacga cgaaggaaag tgcggtgatt ttgaataagc tcaaacaaat cgtgatgtta
    19681 ggtcgtcaat ctggtttctt tctgattcta gcttgtcaaa gaccggatgc gaaatattta
    19741 ggagacggga ttcgcgatca atttaatttc cgggtggctt tgggtcgtat gagtgaactg
    19801 ggatattcca tgatgtttgg cgaggttgat aaaaaattct ttatgaagcg tatcaaaggt
    19861 cgaggctatg tagatacggg agggagtgtg attagtgagt tttatacgcc acttgtacca
    19921 aaaggatatg atttcttaag ggaaattagc gacattggtg gattaaacgt taatacggaa
    19981 ggataaaata atagtatgta gcctaggagt gtcaagaatc ctgggctttt tttacgaggt
    20041 gatttatgac atttttagat ttattcgctg ggattggtgg cttttgctta ggtatggaac
    20101 aagcgggtca ccagtgtatc ggcttttgtg aaattgatga ttttgctcgc cagagttata
    20161 aggcaatcca tgatacaagt aaggaggtgg aaatgcatga catcacaagt gtatcagacg
    20221 agtttattca atctctcgga ccagtggata tcctttgcgg tggatttccg tgccaagctt
    20281 tttcaattgc gggaaagcgg caaggatttt ccgatactcg aggtacttta ttctttgaaa
    20341 tcgctcggtt cgccgctatt ctccaaccta agtttttatt ccttgagaac gtccggggat
    20401 tactcaatca cgaaggaggg gctacgttcg agacgatcct ccgaacgctg gatggattgg
    20461 ggtatgatgt ggaatggcaa gtgcttaact caaaggccta cgttccccaa aaccgtgagc
    20521 ggatcttcct tatcggacat tctcgagacg catgtaccga acaagtattt cctatcattg
    20581 gatcgtctcc aacatctgat caaaacatca gaaacttatt aaatatcaac ccttccaatc
    20641 gaggaatggg gggacaagtc tatgggtcag atgacgtagc gcctaccttg accggtgatg
    20701 aaggaatcaa aatcgcattg ccggtagatg atggaatttc tgtagcgggt atgttactgg
    20761 gaaattttga acaaggcaat cgggtttatg agataactgg aacagcgcct actttgtcaa
    20821 cgaaacaagg cggaacgaag atcatgcttc ggaaaaacga aacagaatat caagaggtga
    20881 agccagggaa cagtgtgaac ctggcttttc ctaattccac aagtcgtcga ggccgtctgg
    20941 gaaaacaaag tgtgcatacc ttattaacag gagaccaaca agcagtcgtc acggatcagt
    21001 atcaaattcg aaagttaaca cctagggagt gctggcggct tcagggcttt ccggactggg
    21061 cttttgatcg agcttctcaa gtcaattcag atagtcagtt gtataagcag gctggaaatt
    21121 ctgtaacagt tccggtcatt tttgatattg ctagaagatt aaaggaggta aaaacagatg
    21181 atttatgatg agttgattgg ggaatctact gggtgattgg caagattcag tcagatccgg
    21241 aattggaaga agaactgtac cggctcaatt ttgatattcg caaaaacggg gtgaaagtcc
    21301 ctgatgatcc gtatgtaatt gatgaggaaa cggatgctcg aatggagctg aatcaagtca
    21361 tcagtgaatt ggaacgaatt gcggactttg ctaaagaacc tgatatccgc caatacttgt
    21421 ttgagataaa agcagagttg gaaattgagg gtattaccgc ggagtaacgg ccaaataaac
    21481 cactgatagg cactgaccgc gggggatttg cgaagcagaa atcctccgtg gtgctgtgcc
    21541 tgtttatgga cggcgttagc cggccatgat gtctcacccc ccgtatctaa caggggggta
    21601 caaaaacaca aaaaataagc aagcaacgat aaggaatggc tgtcctatca aggataataa
    21661 gcaatttgtg tagatgttaa ctgatttgaa aaagttaaca ccttaaattt ggaggtgaga
    21721 acttggcaca aaggaattta gattatcggc tattaaaaga ccggcgtaat gaatatggcg
    21781 tttcacaaaa taaattagct acgacttgtg gacttagtcg cccgtactta aatcagatag
    21841 aaaatggtgg cgtaaccgca tcgacaaaaa ccatgaggaa aatttttgac caactggaaa
    21901 gtttcaatcc cgagttacct ttaacgttac tgtttgacta tgtaaggatt cgctttccca
    21961 caacggatgc gaggaaaatc attcaagaga ttctccattt gaaatttgat tatatgctcc
    22021 acgaagatta cgccttttac tcctatcaag aacaatatgt tatgggagat attgtggtga
    22081 tgttgtcaca tgaagaagat aaaggcgtcc ttttagaatt aaagggtcgt ggttgtcggc
    22141 agtttgaaac ttttttactc gcccaaaagc gtagctggta tgactttttc gaagattgtc
    22201 taaaagccgg tggcgtgatg aaacgcttag acctggcaat caatgatcga gttggcctgt
    22261 tagatattcc tgatttaacg aaaaaatgtc agaaggaaga atgtatctcc ttgtttcgta
    22321 ccttcaaaag ctatcgttcc ggggaactgt tgaaacctga tgataaagat ggtatgggca
    22381 atactttgta tattggcagt cttaagagtg aggtctattt ctgtttgtac gaaaaagact
    22441 atgagcaata tatcaagtta gggattcctt tagaccagac cgaaacgaaa aatcgttttg
    22501 aaatccgact gaaaaatgat cgagcttatc atgcaattca agatttgtta aaaggtcgca
    22561 gtattgaaag taccacattt tccattatta accggtactt gcgatttgcg gataaggtgg
    22621 aaggcaaaag gcgaactaac tggcccttga atgaacaatg ggggcgcttc attggacgga
    22681 atcagaagga aattcaatta acttctgaac cgaagcctta taccattgaa cgaacgttga
    22741 actggcttgg acgacaagtc gcccctacgt ggaagatggc caaggagttg gatcggttga
    22801 atcagacaac gtacattcag gatatggtaa ataatactcg actatcagac cggcacaaaa
    22861 agattttgga gcaacaaagt atggcaattg aaaatttgat tatatgaaaa gaggaatcgt
    22921 gatggaaatt gtgaaagata ttttattggt gtttggtggc agtatgcttg gtgttactgt
    22981 gatgtgcttg atgcatgcaa gtgcagcggc ggaccgagca atggaaaaga aaggaaagtg
    23041 atgaaatgaa tttcggtcaa aattt
//
LOCUS       DANKHL010000024        22655 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000024 DANKHL010000000
VERSION     DANKHL010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.026"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            42..854
                     /locus_tag="PW101_000655"
     CDS             42..854
                     /locus_tag="PW101_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDL2513082.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            851..1651
                     /locus_tag="PW101_000656"
     CDS             851..1651
                     /locus_tag="PW101_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDL2513083.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1812..3080
                     /gene="rseP"
                     /locus_tag="PW101_000657"
     CDS             1812..3080
                     /gene="rseP"
                     /locus_tag="PW101_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDL2513084.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3148..4857
                     /locus_tag="PW101_000658"
     CDS             3148..4857
                     /locus_tag="PW101_000658"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDL2513085.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5069..9427
                     /locus_tag="PW101_000659"
     CDS             5069..9427
                     /locus_tag="PW101_000659"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDL2513086.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9572..10045
                     /gene="rimP"
                     /locus_tag="PW101_000660"
     CDS             9572..10045
                     /gene="rimP"
                     /locus_tag="PW101_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDL2513087.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10068..11243
                     /gene="nusA"
                     /locus_tag="PW101_000661"
     CDS             10068..11243
                     /gene="nusA"
                     /locus_tag="PW101_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDL2513088.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11265..11558
                     /locus_tag="PW101_000662"
     CDS             11265..11558
                     /locus_tag="PW101_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDL2513089.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11555..11866
                     /locus_tag="PW101_000663"
     CDS             11555..11866
                     /locus_tag="PW101_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HDL2513090.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11879..14185
                     /gene="infB"
                     /locus_tag="PW101_000664"
     CDS             11879..14185
                     /gene="infB"
                     /locus_tag="PW101_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDL2513091.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14212..14559
                     /gene="rbfA"
                     /locus_tag="PW101_000665"
     CDS             14212..14559
                     /gene="rbfA"
                     /locus_tag="PW101_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDL2513092.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14716..14889)
                     /locus_tag="PW101_000666"
     CDS             complement(14716..14889)
                     /locus_tag="PW101_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2513093.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            15337..17699
                     /locus_tag="PW101_000667"
                     /pseudo
     CDS             15337..17699
                     /locus_tag="PW101_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18067..18990
                     /gene="truB"
                     /locus_tag="PW101_000668"
     CDS             18067..18990
                     /gene="truB"
                     /locus_tag="PW101_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDL2513094.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18994..19935
                     /gene="ribF"
                     /locus_tag="PW101_000669"
     CDS             18994..19935
                     /gene="ribF"
                     /locus_tag="PW101_000669"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDL2513095.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20287..21072
                     /locus_tag="PW101_000670"
     CDS             20287..21072
                     /locus_tag="PW101_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2513096.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21085..21936
                     /locus_tag="PW101_000671"
     CDS             21085..21936
                     /locus_tag="PW101_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HDL2513097.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22060..22512)
                     /locus_tag="PW101_000672"
     CDS             complement(22060..22512)
                     /locus_tag="PW101_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2513098.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         7686 a         3957 c         4956 g         6056 t
ORIGIN      
        1 cttaagatag tgggaatctt ttaagtatag gaggaaaaag aatgttacgt ttttttccgc
       61 aaaaaaacaa atatattcaa gaaaaaagcg aatttcaatt taataccgat ggaccaattc
      121 ctcagcacat tgccatcatc atggacggca atggtcgttg ggcgcaaaac cgtcgtttgc
      181 cacgagttgc tggccataaa gaaggcatgg aaacagtaaa gaaagtcaca aaaaaagcat
      241 caaggcttgg agtgaaagtg ttgacgcttt atgccttttc gactgaaaac tggaagcgac
      301 caaaagatga agtaagtttt ctcatgcaac tacctgtcga cttctttgat acgttcgtac
      361 cagaattgat taaagaaaat gtcaaagttc atgtcatggg ttatgaaaac gtattgccag
      421 aacatacgca agatgctgta agacgtgcaa ttgagcaaac aaaaaacaat actggaatgg
      481 tattgaattt tgctttgaat tatggaagca gagcagagat cgtgactgct gtccaagaaa
      541 ttgccgaaga agtagcaaaa ggagaaatac acgcagaaga aatcgatgat gaattgattg
      601 ctaagcattt aatgacaggt ttcttgccta aagagttaca agatcctgag ctgatgatcc
      661 gtacaagtgg agaagagcga attagtaatt ttctgttatg gcagatcgca tatagtgagc
      721 tgtattttac aaaagcgctt tggcctgatt ttgatggtgc gcatcttgaa gaagcaatcg
      781 cctcttatca aaatcgagac cgtcgattcg gcggtgtaaa aaaaacagaa gatgagaaag
      841 gggatcagtc atgaaacaac gtgtggtcac agcagccgtg gcgctagtct tgtttattcc
      901 gattatatgg atcggaggca tagcggttga gcttacagca gcaatcttag cagtaatagg
      961 agtttacgag ctgtttcgga tgaaaggatt gacactctta agttttgaag gagttttatc
     1021 cgccgttggt gcagtgttac ttgttttgcc aaaagaaaga tggtttttct ttcttccaga
     1081 gaatgtaagt acatttatgt tattttatct tatagttatg attcttttag gaacatcagt
     1141 tatttctaaa aatacgtata cgattgatga agcaggtttt cctgtgcttg tcagcttgta
     1201 tgtgggaata ggtttccaga attttgtcaa tgcacgaaca gaaagcttgg ctgttttgat
     1261 atttggtcta tttattgttt gggcaactga tatcggagca tatatggttg gtcgacgtta
     1321 tggtcagcat aagttaagtc ctgatatttc gcccaacaaa acgattgaag gtgcgctcgg
     1381 tggtattggt agtgctgttg tagtcgcatt tctttattta ttgctatacc cagcaaaaga
     1441 actatttaat catggtatgt ttgtcatgct tctgatgacg attatttttt cagcagtcgg
     1501 acaatttggt gatttggtcg aatctgcaat caaacgtcat tatgatgtga aagactcggg
     1561 aaatatctta ccaggccatg gtggaatctt agaccgtttc gacagtttgc tgtttgtatt
     1621 tcctatcatg catttatttg gcattattta gtaagtatac ctgcgtttct ttgtgaattt
     1681 cataaaagac gcaggttttt atttttcatt atattgatac tattggtgtc tttggtatac
     1741 tatggtaaaa ttatagtgtt ttatcgttgt tttataaaaa acacaaaaaa caagtaaagg
     1801 agaaaaaagc tatgaaaacg attctgacat ttatcatcgt ttttggtata ttagtgattg
     1861 ttcatgagtt tggtcatttc ttctttgcga aacgatcagg aatcctcgtt cgcgaatttg
     1921 ctatcggtat gggtccgaaa atttatgggc atcaagcaaa agacgggaca acatatacgc
     1981 ttcgcctatt acctattggc ggttatgttc gaatggcagg caatggggat gatgaaacag
     2041 aaatggcacc aggaatgcct ctttctcttt tactaaattc tgacggcatc gtagagaaga
     2101 taaatttaag taagaaaatc caattgacaa atgctatccc gatggaattg agccgttatg
     2161 atttagaaga tgagctgact atcactggtt atgtgaatgg agacgaaaca gaagtagtaa
     2221 cctaccctgt tgatcacgat gcgacgatta ttgaaaacga cggaacagaa atcagaattg
     2281 cgccaaaaga cgtacagttc caatcggcta aattgtggca gcgtatgctg accaactttg
     2341 ctggacccat gaataacttt attttggcaa tcctgttatt catcattctg gcatttatgc
     2401 aaggtggtgt tcaagtgacg aacactaatc gcgtaggaga gatcatgcct aatggagctg
     2461 cggcagaagc aggcttaaaa gaaaatgatg aggtagtcag tgtggacgga aaagaaatcc
     2521 attcatggaa tgatctgaca acagtcatta cgaaaaatcc ggataaaaca ttagacttta
     2581 agatcgaacg agaaggtcag gtgcaatctg tcgacgtgac accgaaatct gtagagtcaa
     2641 atggcgaaaa agttggccaa cttggtataa aagccccaat gaatacaggt tttatggata
     2701 agatcattgg agggacgcgt caagctttca gcggttcatt ggaaattttc aaagccttag
     2761 gatcactatt tacaggtttc agtttagata aattaggcgg acctgtcatg atgtaccaat
     2821 tgtcttcaga agcagcaaat caaggtgtaa caactgttat tagtttgatg gcgcttcttt
     2881 caatgaatct cggaatcgtc aatctgcttc cgattcctgc cttagatggc gggaaattag
     2941 tcttgaatat ttttgaaggg attcgcggga aaccattaag tcaagaaaaa gaaggaatat
     3001 tgacattagc tggctttgga tttttaatgt tgttgatggt gctagttaca tggaacgata
     3061 ttcaacgatt ctttttctag aaaaagttga caaatagggt ataataacaa acgataacta
     3121 ttttgaggaa ataaaggagt atatagaatg agacaatcaa aaatgttgat tccaacatta
     3181 cgcgaagttc caaatgatgc agaagtatta agccatcaaa tcttgctaag agctggatat
     3241 atccgccaag tatcagcggg tatttattca tatctgccat tagcaaatcg tgtattggaa
     3301 aaactgaaac gcattatgcg ggaagaattt gaaaaaatcg gagcggtaga aatgctgatg
     3361 cctgccatct tgccagcaga actttgggaa gaatccgggc gttacgaaac atatggacca
     3421 aacttgtatc gtttcaaaga ccgcaatgat cgaaaaatga tccttggacc aactcacgaa
     3481 gaaactttta ctgatttgat gcgtaatgaa atcaattcat ataagaaatt acctttaaat
     3541 ctttatcaaa tccaacctaa atatcgtgac gaaaaacgac ctcgcttcgg tttgcttcgc
     3601 ggaagagagt ttatcatgaa agatgcgtat tctttccatg caacggaaga aagtttggac
     3661 gagacatata aagattacga aaaagcctac accgaagtat tcaagcgttg tggcttagaa
     3721 tttcgctcga ttatcggaga cggtggcgca atgggcggga aagactcaaa agaattcatg
     3781 gcgatctcag aaatcgggga ggatacgatc tgttattcga ctgaaagtga ttatgctgca
     3841 aacttggaga tggctacaag ctactatgta tcaaaaaaat cgcatgaaac acagcttgaa
     3901 ttggagaaag tagctacacc taacgtaaaa tcaattgacg aagtagcaga tttcttttct
     3961 gttgaacctc aaaaaatcat caagtctgta ttgttcattg cggatgaaca acctgtcctt
     4021 gttttggtac gcggagacca tgaagtcaat gacgtaaaac tgaaaaactt tttatctgct
     4081 gactttttag aagaagcaac agaagaagaa gcacaaaaat atctaggtgc tgagtttggt
     4141 tctgtcggac cagtcggtgt ttcagatgac gtaaaagtat atgcagaccg ttatgtccaa
     4201 gatttagcga atgccattac tggagcgaac gaaacaggat tccactataa aaatgtgaat
     4261 ccagaacgcg atttcactgt actttcttat gaagatcttc gttttgtaca agaaggagat
     4321 ccttcaccag acaacaatgg tgttcttgcc tttactcgag gaatcgaaat cggacatatc
     4381 ttcaaaatcg gcactcgtta cagtgaatct atgggagcaa ctgtactaga cgaaaatgga
     4441 cgagaaaaat ttgtgattat gggctgttat ggtatcggtg taagtcgttt attatcagca
     4501 atcgtagaac aacattcaga tgaacaagga atcaactggc caaaaggaat tgcgccattt
     4561 gatttgcata tcgtccaaat gaatctaaaa gacgaatacc aaacaaacct gacgaatgaa
     4621 ctagaagaag cgatgacaaa agctggctat gaagtactgg ttgatgacag aaatgaacgc
     4681 gcaggagtga aattcgcgga ttctgatttg attggctgtc caatcagaat cactgtcggt
     4741 aaaaaagcag tggatggaat cgtagaagtg aaaatcaaga aaactggcga aatggttgaa
     4801 gtacgtaaag aagaattggt gaatacttta ccaatcctct tgaaccaagg cgaataataa
     4861 gcgttaaaac acagaaaata aaaatagctg aatcagctgt ttttgacaat ctgatgattc
     4921 gcttttaaaa ttggaactgc gatttgaatc gcagttccaa ttttttaatg taaaactcag
     4981 aaaaaacgag tatactaaaa ggtactgaaa aagaagagga taagatgcga tcaattgcat
     5041 tgtttctcat cagaaagggc gtatatcttt gtcagaaaaa cgagatttat ttgaaaaact
     5101 gttggaacaa atccaactag aggaagcaga aaaaaatcat ccactgctta ctgctggtga
     5161 aatggatagt gtagtggtcc atcgtaaatc gcgtttatgg gaatttacgc tgcatttttc
     5221 caaaattttg cctattatgc tatatcggtc attgactcag catctgacta tcgcttttaa
     5281 agatattgca caagtcaaac tgaatatctt agcagatgac cagacattcg atgaacagct
     5341 cttgcaagat tactgggccc aagctttgga aaatcagcaa tgcgatacac cactagcaca
     5401 gcaagtactg aaaacgcagg tacctgtgat aaaagatcgt aaagtcatct tgccaatcga
     5461 cagtacagga gctatttctt atttgaagca acaatacttg ccattagtag aagagttgtt
     5521 cgtcagttac ggattcccaa aatttcgtat cgaaccagaa gtggatgagc agcaagcgga
     5581 acgagtattg aaactttttg aagaacgcaa acaagaacag gcagaagcct ttatgaagca
     5641 agcagctgaa agtctagttg tccacgaaca aaagaaaaag gaaagaaaag agcaatcgcc
     5701 agctcttgaa ggacccatcc acatccagct aggacgaaat attccagccg atgaaccaac
     5761 gacaccaatg atcagtatcg ttgaagaaga acgaagagtc acacttgagg ggtacgtatt
     5821 cgacaaagaa gtcagagaac tacgttcaaa acgcaagatc ttgactttga aaatcacaga
     5881 ttatacttca tcatttatcg tcaaaaaatt ctcgaacggc gaaaaagatg aacaagtatt
     5941 tgatgcgatc agtgtcggta gctggttaaa ggtacgagga agtattcaag aagatacgtt
     6001 tgtccgtgat ttggtcatga atgcgcaaga tatcatagaa gtcaaacata cacctcgcaa
     6061 agattatgct cctgaagggg aaaaacgagt agagcttcat gttcatagca atatgagtac
     6121 gatggatgca acaaacagca tctctgatct tgtggcacaa gcagggaaat ggggacaccg
     6181 agctatcgcc attacagacc acggtggtgc gcaagccttt ccggaagcac atagtgctgg
     6241 aaaaaaagca ggtgtgaaaa tcttatacgg cgtggaagcg aatgtcgtag atgatggtgt
     6301 cccaattgct tataatgatg cacatgaagc attgagtgaa gccacatatg tcgtatttga
     6361 cgtggaaacg acaggattgt ctgcagtata tgacacgatc atcgagctag ccgcagtcaa
     6421 aatgtataaa ggaaatgtga tcgaaagctt tgatgaattt atcgatccag gacatccttt
     6481 gtcacgaaca acggtagatc tgactggtat cacggatgga atggttcgag gatcaaagtc
     6541 ggaagaagaa gtattgcgga tgttccttga attttcaaaa gatacaattt tggtagccca
     6601 caacgcggct tttgatatgg ggttcttgaa tactagctac gcaaggtatg gtattcctga
     6661 agcggccaat ccagtcattg atacgttgga attagctaga tatctgtatc ctcaattcaa
     6721 acgtttcgga ttaggtgtgt tatctaaaaa atttggtgtc agcttggaac agcatcaccg
     6781 agcaatctat gatgctgaag caactggtca cttagcatgg atctttgtga aagaggcaat
     6841 ggacaatcat aacatgcttt atcatgatca gctaaatgag catatcggag aaggagattc
     6901 ttacaaaagg gctcgacctt tccatgtaac gattttagca aaaaatcagg caggactgaa
     6961 agatttgttc aaactgattt caatgtcgaa tgtcgaatac ttcgaacgcg tcccacgaat
     7021 accacgctcc caattgaaaa agatgcgtga aaatctattg atcggctcag cttgtgataa
     7081 gggagaaata ttcgaagcca tgatgcaaaa aggggtagaa gaggcaagaa accgagcaaa
     7141 attctacgat tatattgaag ttatgccaaa agcggtctat gcgcctttga tcgagcaaga
     7201 attggtaaaa aatgaacatg atttggaaga aattatccaa aatttggtag aaatcggaaa
     7261 aagtttggat aaaatcgttg ttgcaacagg taatgtacat tatctgaacg aagaagatgc
     7321 catttaccga aaaattttga tcaattctat gggcggagcg aatccgttga atcgtcatag
     7381 tttgccggat gtccatttcc gaacaaccga tgagatgcta actgcatttc actttttagg
     7441 ggaagaaaca gcgaaagaga tcgtagtaga aaatacgaat aaaatcgcag atatctgcga
     7501 agaagtgatt ccagtcaaag atgaacttta tacaccgaag attccaggtt cagaagatga
     7561 aatatcagaa ttgagttaca caaaagcaaa acaaatgtac ggagatccgt tacctgagat
     7621 cattcaaaaa cgattaaaaa aagagttgaa ttctattaat ggtaatggct tctcggttat
     7681 ttatctgatc gcccaaaaac tagtgcataa aagtaatgaa gatggctatc tagtaggttc
     7741 acgtggctct gttggctcta gctttgttgc gacgatgaca ggaatcactg aagttaatcc
     7801 gttagcccct cattattatt gtccagagtg ccaatattcc gagttttttg aagatggtac
     7861 gtatggttct ggatttgaca tgccagaaaa acaatgtcca aaatgtggtg cacgcttaaa
     7921 caaagacggt catgatattc catttgagac atttttagga ttccatggag acaaagtgcc
     7981 cgatattgat ttgaacttct caggtgatta tcaagcagaa gcccataact atacgaaagt
     8041 attgtttggc gaagactatg tttaccgtgc cggaacgatt ggtacagtcg ccgacaaaac
     8101 agcatatggc tatgtaaaag gatacgaaag ggacaataat ctgcagtttc gaagtgcaga
     8161 agtcgacaga ttagcaaaag gcgctactgg agtcaaacgg acaaccggcc agcatccagg
     8221 ggggatcatc gttattcccg attatatgga tgtctatgat tttacgccga tccaatatcc
     8281 agcagacgat caaaattcgg aatggaaaac gactcatttt gattttcact cgattcacga
     8341 taatgtattg aaattagata ttctagggca tgatgaccca actgttattc ggatgcttca
     8401 agatttatca gggatcgatc cacaaacgat ccctacggat gacccggaag tgatgcggat
     8461 atttgccggt cctgaagtac ttggagtaag tcaggaacaa atttattcca aaacagggac
     8521 attaggtatt ccagaattcg gcactcgttt tgttcgaggg atgctagaag aaacgcatcc
     8581 tacaactttt gcagaactac tgcaaatttc cggactatct catggtacag acgtatggtt
     8641 aggaaatgcg gaagaattga ttcgacgagg agatgcgaca ctggcggaag taatcggctg
     8701 tcgtgatgat attatggttt atttgatcca tgctggatta gatagcggga tggcatttaa
     8761 aatcatggaa actgttcgta aaggacaatg gaacaagatc cctgatgaat tgcgagaaac
     8821 ctatctatcg gcgatgaaag aaaataatgt gcccgactgg tatatcgatt cttgttcaaa
     8881 aatcaaatat atgtttccga aagcccatgc tgcagcttac gtattgatgg ctcttcgtgt
     8941 cgcttatttc aaggtttatt tccctatttt gtattactgt gcatactttt ctgtacgtgc
     9001 agacgacttt gatctagtct ccatgtgcaa aggaaaagat gctgtgaaac aagcaatgaa
     9061 agaaatcacg gacaaaggat tagatgcttc cgtaaaagaa aagaatcaac tgacagtgct
     9121 ggagttagcc aatgaaatgc tggaacgagg attcaaattt ggaatgattg atttgtataa
     9181 atcggatgcc gttaactttg ttattgaagg tgatacgttg atcgcaccat ttagagctgt
     9241 cccaagtttg ggaaccaacg tggcaaaaca aatcgttgaa gcaagaaaag acggtccatt
     9301 tttatccaaa gaagatcttg ccacccgcgg taaagtttca aaaacattga tcgaatatat
     9361 gaacgacaat ggcgtattga aagatctacc agatgaaaat caattatcgt tattcgatat
     9421 gctataagca tctgttttcc tgtctgcttg taattttaag cagagtatgg tatagttctt
     9481 ataggtaatg atactaactg tgagtgagcg gatttttcgc tcactctttt taatggaatt
     9541 acgcacttaa ttttttaagg aggcgaaact tttgagtagc gtcgttgaaa cagtcacaga
     9601 aatggtgaca ccgattttag atgaacaaaa ttttgaactt gtcgaagtcg aatttgtaaa
     9661 agaaggaaaa agctggttct tacgagtatt tattgacaaa gaaggcggta tcgatatcga
     9721 agaatgcgcc tttgtcagtg agaaactgag tgaaaaactt gatgcagcag atccagatcc
     9781 gattcctcaa gcttatttcc tggaggtatc ctccccggga gcagagcggc cattgaaaaa
     9841 agaaagcgac tatgaaaagg cacttggtga ttatgtccat gtctccttat accaaccagt
     9901 agatggcgaa aaacaatatg aaggatttct tcaatcattt gatgctgaac agcttacatt
     9961 gaaaatacgc ataaaaacac gagaaaaaga attgactttt gaccgaaaaa atattgcaaa
    10021 agctcgctta gctatccaat tttaatcacg gagaggaatc agaaaaaatg agtaaagaaa
    10081 tgttaaatgc gttagacgct ttagaagctg aaaaagggat ctcaaaagaa atcgtgatcg
    10141 atgctttaga agcagcattg gtttctgcat acaaacgtca ctatggccaa gcacaaaacg
    10201 tcgaagtgga atttgatcag aaaaaaggaa agatccatgt ctatgcggta aaagaagtaa
    10261 cggaagaagt gatggattca cagttggaag tctcattgaa agatgcactt ttaatcaatc
    10321 cagcatatga gatcggtgat acgatccgtt ttgaagtaac accaaaagat tttgggcgga
    10381 tcgctgcgca aacggctaaa caagttattt tacagcgtgt acgtgaagca gaaagaacga
    10441 ttatttataa cgaattcagt gcttatgaaa aagacatcat gcaaggaatc gtcgaacgcc
    10501 aggataaacg ttatatttat gtcaatcttg gtaaaattga ggcagttctt tctaaacaag
    10561 atcaaatgcc aaatgaattt tatcaaccac atgatcgaat caaagtttat gtttcccgag
    10621 tagaaaatac gtctaaaggt ccgcaagtat ttgttagccg cagccatcca gacctattgc
    10681 gccgcttatt tgagcaagaa gtaccagaag tctatgatgg aatcgttgaa attgtcagca
    10741 tcgcccgtga agctggcgat cgttcaaaag tggcagttcg ctcaaatgat ccaaatatcg
    10801 atgcagtcgg tacatgtgtc gggccaaagg gacaacgagt tcaagcaatc gtcaatgaat
    10861 taaaaggcga aaacatggat atcgtcgaat ggaatgaaga tccagctgta ttcattgcaa
    10921 atgcattgaa tcctgcacaa gtcatggatg tgatcttcga tgagacaaat ccaaaggcct
    10981 gcacagttgt cgttccagac taccaattat cgctagcaat cggtaaacgc ggacaaaacg
    11041 cacgtttagc tgctaaattg actaaccata aaatcgatat caaatcagaa tctgacatga
    11101 cggaatttta tgaaaatcaa gaacaacaaa aagaaacaga agaactgcat gatgaagcaa
    11161 tcgttcagtc agatttaaca gacgatgaat atgaaacaat tgcattcaac aatgaaacgg
    11221 tcgaagaaaa accagaagct taactttagt aaggaggcaa agagatgaaa caaaggaaaa
    11281 ttcctttacg caaatccgtg gtttcaggtg aaatgaaacc taaaaaagag atggtccgaa
    11341 tcacccgttc aaaagaaggg gaagtatcga tcgatccaac tggaaaaatg cctggacgag
    11401 gagcgtatgt ctcacttgag ccggaagaag tacaacaagc ttgggataaa aaaattctcg
    11461 atcgagtact cgaaacaaca ttaacagatg agttttatca agaacttctt gactatgtca
    11521 ctcaccaaaa agcacgtaaa gagttatttg gaaaatgaat acagttaatc ggcaaaaagc
    11581 catgaatctc atcggtctag cgatgcgcgc gggaaaacta atcacgggtg aagaactgac
    11641 cattgctgaa atccgcaaac aaaaagcaaa attggtgttt gtggcaaccg atgcaagcga
    11701 gaatacaaga aaaaagataa aagataagag ttcatattat gaagttcctt gctttgagct
    11761 attttctgaa gcagaaatca cgcagatgat cggaaaacct cgcaaggtat tcggaataat
    11821 ggatgccgga tttgcgaaga agaccaagga gctaattgaa ggttaggaag gtgattgcat
    11881 gggcaacaaa agaatctatg aattagcaaa agaactcaaa aaatctagta aagacgtcgt
    11941 ggaaaaagca caacaacttg gcatcgatgt gaaaaaccat atgggtgcga ttactagcaa
    12001 tgatgagaag aaattgcaac aagcgttcaa aaacccaagc acgaaacaac aagctgcaca
    12061 acaagcaagc agaaaaccag ccaatcaaca gccgaacgat caacgtaaaa aagaaacaac
    12121 gaacaatcaa caaaaaaata aaaataaccg cagttatcaa gatcgcggtc aagggagcgg
    12181 tcaagtgaat caaggaaaaa aaccaacaac aaaccaaaat aatacgggaa ataccagcag
    12241 ccaaaatcgt caaggaacga gccaaggcgg caatcaaaac cgtactaaca ataacaacaa
    12301 accaaacaat aacaatcgcg gcaaatttaa taataataac cgtaatcgtt ataataaaaa
    12361 aggtaaaaaa ggcaaacagc aacaatcaac taaaccagct gtaccaccac gcaaattccg
    12421 tgaattgcca gatgtattag agtatactga aggtatgaac gtagcagata ttgcgaaaaa
    12481 aattcatcgt gagccagcag aaatcattaa aaaattattt atgcttggtg tcatggtcaa
    12541 tcaaaaccaa gcactggata aagatacgat tgaactgtta gcgacggact atggcatgga
    12601 accacaagaa aaagtccaag ttgacattgc tgatatcgat aaattcttcg aggcagaaga
    12661 agtcgatcca gataaattag tctcacgtcc gccagtcgta acgattatgg gacacgtaga
    12721 ccatgggaaa acaactttat tggatacatt acgccattca cgtgtaacaa gcggcgaagc
    12781 cggcggaatc acacagcata tcggtgccta ccagatcgat attgatggta aaccaatcac
    12841 tttcttggat acaccaggac atgcggcctt tacaagtatg cgtgcacgtg gagcaagtat
    12901 cacagatatc acgatcttag ttgttgctgc agatgatggc gtcatgccac aaacagttga
    12961 agcaatcaac catgccaaag ctgcaggcgt tccaatcatc gtcgctgtaa ataaaattga
    13021 taaaccaggt gcaaaccctc aacatgttat gcaagaatta agtgagtatg agttaattcc
    13081 tgaagcatgg ggtggagaaa cgatctttgt tgagatctca gcgaaattcg gccaaaacat
    13141 cgaagaattg ctggaaatga tcctgttagt cgcagaagta gaagatttaa aagcagatcc
    13201 aacacaacgc gcaatcggaa cagtgatcga agcacgtctt gataaaggta aaggtcctgt
    13261 gtcaacctta cttgtccaag aaggaacact tcatgttggt gacccaatcg ttgtcggtaa
    13321 tacttacgga cgtgtccgtg taatgacaaa cgatcttggc cgtcgcgata aagaagcagg
    13381 acctgcaacg ccagtagaaa tcactggctt gaatgatgta cctcaagcag gtgaccgttt
    13441 cgtagtcttc gaagacgaaa agacagcacg tgcagcgggt gaagaacgcg cgaaacgtgc
    13501 attattagaa aaccgtgctg ccacaagccg tgtgactttg gataacttat ttgaaagcct
    13561 gaaagaaggc gaattgaaag aagttaacgt aatcatcaaa gcagatgtac aaggttcagc
    13621 agaagcactg gctgcatcat tgaagaagat tgatgtggaa ggtgtccgtg taaaaatcgt
    13681 ccattcagct gtcggagcaa tcaatgaaag tgatgtaacc ttagcagcag ccagcaacgc
    13741 tattatcatc ggtttcaacg ttcgtcctac accgcaggca aaagtgcaag ctgatactga
    13801 agaagtcgat attcgtcttc accgtatcat ctataaagcc atcgaagaaa tcgaaactgc
    13861 gatgaaaggg atgctagacc ctgaatttga agaaaaaatc accggtcaaa tgatcgttcg
    13921 tgaaaccttc aaagtttcta aagttggtac gattgcaggt gcctatgtaa cagaaggata
    13981 tattcgtaga gatagcggtg tccgtgtaat ccgtgacgga atcgtaatct atgaaggcca
    14041 acttgcaagt ctgaaacgat tcaaagatga cgtgaaagaa gtcaaaatgg gttatgaatg
    14101 tggtgcaatg attgaaaaat tcaatgacat caaagtagat gacgtaattg aaggatttat
    14161 catggaagaa gtcaaaaatg actaaaaact gataaaagga ggccaatcat tatggctaac
    14221 taccgtgacc gccgagtcgg tcaagaaatc ttaaaagaag tcaatgatat tttgcgcaaa
    14281 aaggtccgtg accctcgtgt ggagaacgta acgatcacag atgtgaaagt tacgggggac
    14341 ttacagcaag caacgatcta ttacagcatt ttatctgact tagcatcaga taagaaaaca
    14401 gcacaagatg gattgaataa agcatcagga ttgattcgtc gagagttagg gcacaatcta
    14461 agtatttata agacacctga attgttcttt gaacttgatg aatcagttgt ctatggcaac
    14521 catatcgatg aattgcttcg cgacttgaac aaagactaat caaaaactcc cgcaaatcct
    14581 gtgaaatcag gggttgcggg atttttttgt tttcattaag acgtattacc taagagttcc
    14641 atgactttag tcttgcggcg tgtcagctaa ccaaaaaaga gaaaaagaaa agtaaaaaat
    14701 tcgactttct tttttctagt agtatataaa tttttgcata tagataaaga tcaagatacc
    14761 gataacggaa gctagattgt aatctatttt agtgacagtc aggtatagcc aagtaactag
    14821 aagatcagtg gaacctgcac cataaaagtt tgagtttgtc ggtccgttga cagttaaaag
    14881 aaaaatcacg ttgaagttat tgatatttcc gattacgccg cttcgatttg gtcacgatcc
    14941 aaattttgga taatccctgt agaaattagc atagtagctg ggataccaat tcacatattg
    15001 acaaagataa cagagacttt tacataaaac ggatcaatca ggaaaggaat tgctcgatcc
    15061 agccccagtt ttccagcaat gtgttaactg gcccagcgtc attcagcaaa ttatgcatga
    15121 tgaggagcga aatgaactgc ggaacagcca ttgtgataac gacaagtttt ttggatcaat
    15181 gtaaaaaacg tcagcgaaag gaaaaatttc gtggtttttt atacctgttc atttggctag
    15241 atataaaatc caaacaaatg aacagaagtg cttattaggt ttcaattggg agtatctgcg
    15301 ctagaataag agtaataatg aaaaggagtg ttatgtatgg attattctag taaagaatac
    15361 tttgataaaa tgacggcttg gtggcgtgct gctaattatc tttctgttgg gcaactttat
    15421 cttaaagata accccttact gagacgtaca ctgaaacccg aagatgtcaa aaaacatcca
    15481 atcggtcatt ggggaacgat tcctggccaa aattttattt atgtccattt gaaccgagtc
    15541 atcaataaat atgatttgaa catgttttat attgaagggc caggacacgg cggacaagtc
    15601 atggtatcga atgcttattt agatggaagt tacacagaga tctatccaga agtaacggaa
    15661 gatgagacag gaatgcaaaa attattcaaa cgattttcat ttcctggagg cattgcttct
    15721 catgcagcac cagaaacacc aggttctatc catgaaggcg gagagcttgg atactctctt
    15781 tcacatgcag tcggagctgt attagacaat cctgaagtaa tctctgctgt cgtgattggt
    15841 gatggtgaag cagaaaccgg tcctttagct ggaagctggt tctcaaatgt ctttatcaat
    15901 ccagtgatag atggagctgt tttaccgatc cttcatttaa atggtgcaaa aatagccaac
    15961 ccaacgatct tggcacgtaa atcagatgga gaattagcaa attactttaa cggacttggc
    16021 tgggaacctt tctttattga aggaaatgat tcagaaaaat taaatcctgt tatggctgaa
    16081 aaaatggatc aagcaatcga gaaaattaaa tccatccaaa aagaagctcg cctgaagaca
    16141 gcaacagatg tcgtgatgcc aaaatggcca gtattgatcg tacgcacacc aaaaggatgg
    16201 accggtccaa aagaatggga cggggaacca attgaaggaa ccttccgagc acaccaagta
    16261 ccgattcctg tagatcaaga acatatggat catgcagatg ctttactccg ttggctgaaa
    16321 tcctatgaac cagaaaaatt atttgatgca caaggacgta ttttagaaga aatcagagaa
    16381 atcgcgccta ctggtgacca aagaatggca aaaaatccta tcaccaatgg tggaatcgat
    16441 ccaagaccgc ttattatgcc agattggaaa aaatatactt tgcaatttga aaaaccaggc
    16501 tccgttaaag cagaagatat gaccgaactt ggtaaattcg ttcgcgagat cattgagaaa
    16561 aatccagaaa atttccgtat atttggacca gatgaaacaa aatcaaaccg attgaatcaa
    16621 gttttcaaaa cgacaaatcg ccaatggatg gaaaaaatcg aaccagaaaa tgatgaatgg
    16681 ctttcgccat ctggtcgcgt catcgacagt caattatcgg aacaccaaga tgaagggttc
    16741 ttagaagggt acgttctgac tggacggcat ggttttttgc aagttacgaa tcattcttgc
    16801 gagtggttga ctctatgttg actcagcact tcaaatggat gcggaaatca catgaccttt
    16861 cttggcgaaa tgattatcct tcgctaaatc tcattgcaag ctcaacagtc ttccagcaag
    16921 atcacaatgg atatagccac caagatccag gaatattgac acatttggca gaaaagaagg
    16981 cggagttcat ccgtgaatat ctgcctgcgg atgcaaatac attgctcgca gtcatggata
    17041 aagcattccg ctcgtcagaa aaaatcaatt tgatcatctc ttccaaacat ccgcgtgctc
    17101 aattttactc agcagaagaa gcagccgtct tagtgaatga aggcttgaaa atcattgatt
    17161 gggcttcgac agcaaaagag gaggaacctg aattggtcat tgctgctgca ggaacggaat
    17221 ctaacttaga agcattagca gctgtaactt tgctgctgga agaattccca aaactaaaaa
    17281 ttcggttcat caatgtcgta gatttattga aattacgtca tccaagtcaa gatccgcgtg
    17341 ggttgagcga tgaagaattt gatcaatatt ttacaaaaga caaaccgatc ttatttgctt
    17401 tccacggtta tgaaacattg gtccgcacga ttttctttga tcgtcataac catcatttaa
    17461 tgattcatgg ctacaaagaa aatggagata tcacgacacc atttgacatg cgtgtagtaa
    17521 atgaacttga tcgttatcat ttggcaaaag acgcagcttt aaaaatcaaa ggaagtcaag
    17581 cagaagattt tgcgaaaaaa atggatcaga aattacaaga acatcaaaac tatattcgtg
    17641 aaaatggtat tgatctgcca gaagtattgg attggaaatg gaaaaatctt gaccaataaa
    17701 atgataaaat cgatcccgca aaatagaatt ttttacattg attaagaaac tgaaaaaata
    17761 aataaaaaaa acggaaaaaa gactggaaga atggccagtc ttttttttcg tttttgggaa
    17821 gtgaaaacca aactatttac ctgtaagcca cccataaatc caatcaaatg gtgcaaagaa
    17881 aaaatcttcc attctattcc ctcctttaaa aaagcataac atgatttcta tttacgtgag
    17941 catgaatttt gttacgtttt tattgccgaa aaataaaaac cgttcattat gatgtcaaat
    18001 tcgtcagtaa ttgatgcaat gtgttataat aattcagtta aatcaaaaat gaaatggaga
    18061 aaagacatgg acgggttatt acctttatgg aaagaacgcg gaatgacaag tcatgattgc
    18121 gtattcaaat taagaaaaat cttacatacg aaaaaaatcg gacatggggg tacgcttgac
    18181 ccagacgtag acggtgtgtt gcccatttgt attggaaaag ccacaaaagt tatcgaatat
    18241 ttgacagatt ctgggaaaat ctatgtaggc gagataactc taggtttcag tacaacgacg
    18301 gaagatcgat ccggagaacg ggtagaaaca aaaccagtag atatgtcttt atctgaaaaa
    18361 aaaatagatg aagtgatggc ttcatttatt ggtgagatca cccagatacc accaatgtac
    18421 tcagcggtaa aagtcaacgg taagcggtta tatgaatatg cccgcaataa tgaaacagtg
    18481 gagcgtcctg ttagaaaggc tcagatttac cgttttatcc ggacaagtga attgacaaga
    18541 gacgaagtga caggaaccat ttcttggcgc ttcgaagtag aatgcgggaa aggaacctat
    18601 gtccgcacat tagctgtaga tacaggcaag aaacttggtt accctgcgca catgtctgat
    18661 ctgacccgaa cagcaagtgc cggtatgtcc cagaaggatg ctctgacatt agaagaagta
    18721 gctcaagctg tgaaaaatgg gacaatcgat gattgtttgc tgccaataga aaaaggagtg
    18781 gaacggttca aacgagtaga tattactgat gaagtatggc agaaagtaaa aaacggtatg
    18841 agacttgatt atactgtatt tgcgctccca gaaatgccgt cagaagaaat tgctttgttt
    18901 tataaaggaa aagttgtcag tatttatcag ccaaacccga aagaaaaaaa caaattaaaa
    18961 cctagtaaag tattgagaaa tgaggaataa cgaatgaata tcatcaagat cagacatcct
    19021 taccaatcag aacaaattcc agaagacgat gtcgttttag tgctgggatt tttcgacggc
    19081 gtgcatatcg gacaccagaa agtcataaag acaggtatgg aaattgcaca aaaagaagga
    19141 ttgaaattag cattgatgac tttcaatcag catccatcta ttgtctttaa aaagatcgat
    19201 cctagcagcg tgaaatattt gactagcctt aagcaaaaag aagaaaaaat ggccgctttg
    19261 ggtatagatt atttatatga agttgatttt acttcttctt ttgctcattt agcaccgcag
    19321 gaatttgttg atcaatacat cgtagggtta catgcaaaat atgctgtttc cggttttgat
    19381 tatacgtatg gaccaaaaga cattgcagat gtcgctcatt ttcctgaata tgccaaagga
    19441 agattcaaaa tcgtcacagt ttctaaagaa gaagaacaag gtgaaaaaat cagctctacc
    19501 cgtattcgtg gattactaga cagcggacaa gtaaaagaag cagcaaaact attaggttgc
    19561 acatatgagg tagaaggggt agttgtccat ggcgatgcac gaggcagact actaggattc
    19621 cctacagcaa atgtcaaagt aaagagcacg gttcatttgc ctaaagaagg tgtttatgtg
    19681 tcggaaataa aaattgggga aacatggtat ccagccatgg gttcaattgg acataatgat
    19741 acattcggtg aaggacggga gctgactgta gagttgtata ttttggactt taatcaagat
    19801 atctatggtg aacatgtcgc ggtacgctgg aatcaatttt taagagatca agtgaaattt
    19861 tctagtgtag aggaattgat tgaacaactc aaggaggatg aacaagcaac agctgaatat
    19921 ttttctcagc aataaaacat tatgcataaa atctcttgtt tatgtataac tagcaattac
    19981 aataaaatgg gtgataaata agcccaggaa aggggctgtg acaaaagtct tgtcacagcc
    20041 tttttttcca aataaatggt ttttcagaag tagcttcttc ggaaataagc cgaaatttct
    20101 tgaagctgac cacttctgtc acaacctctt tctttataga aataaaagga cactagcaat
    20161 tcatgaaaac gattgctaga gcgtatcatt tgctttagac tatatatctt ggtgtacact
    20221 cactttaagg cggaaataaa agttatccgt aataacaggt ttaggtatga aaaaggagtg
    20281 ggtactatgt acaaggaatt agaaaacaaa gtcgcagtag taactggtgg ctcaaagggg
    20341 attggaacag cgattgctca acgattcggg aaagaaaaaa tgaatgtagt tgtcaactat
    20401 aactcggata aagaaggagc agaaaaagca gtcaaagcaa tcgaagaggc aggcggaaaa
    20461 gcagtctctg tacaagcaga tgtcagttct gaagaaggaa tacagcgttt acttgatacg
    20521 gctttagaaa attttgggac actggacgtt tgggttaata atgctgggat ggaaaatcag
    20581 catccaaccc atgaacttag ccttgaagat tgggaaagag tgttaaaagt caatttgact
    20641 ggcgttttta tcggaacgaa agcggcttta aattacttta cagagaataa taaaaaagga
    20701 aatatcatca atatgtcttc tgttcatgaa cagatcccat ggccaacgtt tgctcattac
    20761 gctgcttcaa aaggcggggt aaaattattt accgaaacgg tagcaatgga gtatgcaccg
    20821 aagggaatca gaatcaatag tataggccct ggtgctatcc gtacacctat taatgcggca
    20881 aaatttgatg atcctgaaca aaaagaactc ttggaaagta tgatcccact aggacgcgta
    20941 ggtgaaccaa aagaaatagc ggcgacagct gcatggttgg cttcagacga atccagttat
    21001 gttacaggta ttacactatt tgcagatggt gggatgactt tatatccttc tttccaaggt
    21061 ggaaaaggat aagggtgaat aaaaatgggt ttattgattg cattgatccc ggcagttggc
    21121 tgggggatcc agccactgat tttgaaaaag attggtggca gaccaacaaa tgagatatta
    21181 ggaactggta ttggagcggt gcttgtcggc ttactcgttc aattattcgc ttctccagct
    21241 ggtatttcag tgactacatt tttgattagt ttactttctg gtgcattttg ggttattgga
    21301 caaacaggac agtatacatc tttcaacttg attggtgttt ctaaaacgat gccaatttcg
    21361 actgcattac agttagtcgg aacctcgttg attggtgtct tcgcttttgg agaatggtct
    21421 ggaagtatgg ggaaaatcat tggagcagca gcaatcattc ttcttgtatt tggttctgct
    21481 ttaacggcaa tcagtgatga tcctggaaaa aaaggcggta tcacaaaagg aattagtcta
    21541 ttagcatcaa caagtattgg ttattgggta tacagtgcat tgcctaaact agttgatgca
    21601 agcggacttt ccattttctt tccgcaaatg atcggtgtct ttttaggggc agtgatttat
    21661 gtgattttcc gtaaaccaac cgcattttct gaagacaaga gttggaaagc aacgatcgtt
    21721 ggagccatct ttagtgtgtc agcattggct tatatttttt cagcaaatga aaatggggtt
    21781 gctactgcat atatcattac acagctaaac gtagttatct ctacattagg cggtatgtac
    21841 atcctgcatg aaaataagac accaaaagaa aaaaaattaa cgattatcgg attattgctg
    21901 atcgtagtag gaagcatggt cacggtcttt atttgaggat aaagatggta aaatttcatt
    21961 tattaaaaaa atatttgttt gaaccaaaca gtaagtactt ataaaaaaat agcgctagaa
    22021 tttccaacat ctctggaatt tctagcgctt tttaattgtt taagatacaa gggaaatatc
    22081 tgtctggatc aataattttt cattgatcca ttgactaaag tttacgacat aattgaagtt
    22141 accaccgatt agtatctccg catgattgtc tttataaaga tcgtattgta aaagacggtc
    22201 ttttttattt gcgtaaacat cgttatggta ctggactaat tgttctcgga attgattgat
    22261 tgctgtaaaa tcacgatatt gaaggttgaa acgcatcgtg attggtgcat ttagcatgaa
    22321 tcgctgcatt tcttttgctg cttcctctgc ttgatctccg tataggaact caaaaagatt
    22381 acgttcagaa aatttccatt gcccgccaac ctttgttcct tttagttttc cttcatgaag
    22441 ataatttctg atagaacgtg tagtaacacc taattgttcg gctaattcat ttactgagta
    22501 aaaatctttc aatgttcacc ctcctatgtg aatggttcta ctgacttagt tgatatttgc
    22561 ttaatcacga aatgtagaaa gaacgatttt aattaaggtc attgtaaaaa tcaagtcaga
    22621 aaattctaac ttttatttct ctaaagaaat tcgtt
//
LOCUS       DANKHL010000025        22336 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000025 DANKHL010000000
VERSION     DANKHL010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.027"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..1162
                     /locus_tag="PW101_000673"
     CDS             <1..1162
                     /locus_tag="PW101_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2513099.1"
                     /translation="GIWSYAAPKHTLEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAG
                     ESVEYLPNEHYWNGTPKLKKITMKSVPMASATEALNSKLYDMIFQMPTDTYDTYKEIA
                     GYTNLGREQTSYTYLGFKLGKWNADTKSVEYNPDSKMADKSLRQAMGYALDNNAVGER
                     FYAGLRSNATSLIPPVFQSYHDDEMKGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDG
                     NKLTINFASMSGGETAQPLADYYIQQWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEI
                     DVYQAAWNTGTDPNPSGLWGPNAAFNYTRYESEENTKLINDISSSKAFDEEYQKEAYQ
                     KWQQYAFEEAFAIPTLFRNEVLPVNDRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            1510..2196
                     /locus_tag="PW101_000674"
     CDS             1510..2196
                     /locus_tag="PW101_000674"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDL2513100.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            2216..5797
                     /gene="smc"
                     /locus_tag="PW101_000675"
     CDS             2216..5797
                     /gene="smc"
                     /locus_tag="PW101_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDL2513101.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            5806..6615
                     /locus_tag="PW101_000676"
     CDS             5806..6615
                     /locus_tag="PW101_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2513102.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            6626..7624
                     /gene="ftsY"
                     /locus_tag="PW101_000677"
     CDS             6626..7624
                     /gene="ftsY"
                     /locus_tag="PW101_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDL2513103.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            7811..10078
                     /gene="gshAB"
                     /locus_tag="PW101_000678"
     CDS             7811..10078
                     /gene="gshAB"
                     /locus_tag="PW101_000678"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDL2513104.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(10167..10619)
                     /locus_tag="PW101_000679"
     CDS             complement(10167..10619)
                     /locus_tag="PW101_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HDL2513105.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            10769..11719
                     /locus_tag="PW101_000680"
     CDS             10769..11719
                     /locus_tag="PW101_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL2513106.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            11712..12671
                     /locus_tag="PW101_000681"
     CDS             11712..12671
                     /locus_tag="PW101_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL2513107.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            12668..13423
                     /locus_tag="PW101_000682"
     CDS             12668..13423
                     /locus_tag="PW101_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2513108.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            13446..14399
                     /locus_tag="PW101_000683"
     CDS             13446..14399
                     /locus_tag="PW101_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2513109.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(15043..15339)
                     /locus_tag="PW101_000684"
     CDS             complement(15043..15339)
                     /locus_tag="PW101_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HDL2513110.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            15694..16050
                     /locus_tag="PW101_000685"
     CDS             15694..16050
                     /locus_tag="PW101_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2513111.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            16060..16959
                     /locus_tag="PW101_000686"
     CDS             16060..16959
                     /locus_tag="PW101_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDL2513112.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            17476..18207
                     /locus_tag="PW101_000687"
     CDS             17476..18207
                     /locus_tag="PW101_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2513113.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            18352..20379
                     /locus_tag="PW101_000688"
     CDS             18352..20379
                     /locus_tag="PW101_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL2513114.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            20487..21584
                     /locus_tag="PW101_000689"
     CDS             20487..21584
                     /locus_tag="PW101_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL2513115.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            21891..>22336
                     /locus_tag="PW101_000690"
     CDS             21891..>22336
                     /locus_tag="PW101_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2513116.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT         7591 a         3796 c         4665 g         6284 t
ORIGIN      
        1 tggtatctgg tcatatgcag caccaaaaca tacgttagaa ggtgtagcaa tcaaagatat
       61 ggaatcaagc gatcaagtac gtaaaaatcc agtgactttt ggcccttatt acatgagtaa
      121 tatcgttgca ggagaatcag tagaatactt gccaaacgag cactactgga atggtacgcc
      181 taaattgaaa aagatcacga tgaaatctgt accgatggca agtgcaactg aagcattgaa
      241 ctctaaatta tatgatatga ttttccaaat gccaacagat acttacgata cctataaaga
      301 aattgctggc tatacaaact taggccgtga acaaacttct tatacttatc taggattcaa
      361 gttaggtaaa tggaatgcag atacaaagag tgtggaatac aatccagatt caaaaatggc
      421 tgataaatca ttacgtcaag caatgggcta tgcattagat aataatgcag taggtgaacg
      481 tttctatgca ggtttgagaa gcaatgcgac ttcattgatt cctccagtat tccagtcata
      541 ccatgatgat gagatgaaag gattcacaca agatctagac aaagcgaaaa aactattaga
      601 tgatgctggc tacaaagatg tggatgacga tggtttaaga gaagacaaag atggaaataa
      661 actaacaatc aactttgctt cgatgtccgg tggggaaaca gcgcaaccat tggcagatta
      721 ctatatccaa caatggaaag aaattggttt ggatgtccaa tacacaacag gtcgtctgat
      781 cgaattcaat tctttctatg ataaattaga aaacgatgat cctgaaattg atgtttacca
      841 agctgcttgg aatacaggaa ctgatccaaa tccaagtgga ctatggggac caaatgctgc
      901 gttcaactac actcgttatg aatcagaaga aaatacaaaa ttgatcaacg atatcagttc
      961 aagcaaagca tttgatgaag agtaccaaaa agaagcatat caaaaatggc aacaatatgc
     1021 atttgaagaa gcatttgcga ttccaacgtt gttccgtaat gaagtattgc cagtcaacga
     1081 ccgtgtcaaa gactgggatt ggtcttatga tgtaccaaac ccatgggcaa atgtttctgt
     1141 tacagcagat tcacgttcat aatcactaaa aatgaccgaa aaagggcgat gtcctttttc
     1201 ggttttttga tatttttctg tttatttctc taatattcag gtcaaggcgc tgatttcatg
     1261 acttctccaa taaacggtgt tcaaagtcca gtttctcggt attaagtcaa atttatgcaa
     1321 aaatatggaa agccattttc acaaaatttt ttcttcatac tcgaaactaa acgaattttt
     1381 cacaacttct cgtcaatcac taggattatt aaagctttac ttaccttttg accgaaccat
     1441 ttcaaatttg acaaaaaacc agtataataa ggaagtatcg aaaaaatatc cataaaagga
     1501 atgtggaaaa tggacaacca gctgacaaaa gaactaaaag aaaactataa catcgttttt
     1561 cacgatctaa atttgcttga acaagctttt acccattcat cctatgtgaa tgagcatcgc
     1621 aatctgcaat tatcggataa tgagcgactt gaatttttag gagatgccgt tttagaactg
     1681 atggtttctc agtatttatt ccgtttatat cctcatttgc cagaaggaaa attgacaaaa
     1741 acacgagctg cgatcgttcg cgaagacagc ttgtcgaaat ttgccaaaga atgtcacttt
     1801 gatcaatatg ttttgttggg caagggagaa gagaattcag gaggacgtac gcgaccagca
     1861 ttgctttgcg acctattcga agctttttta ggcgcgttat atcttgacca gggattcgac
     1921 acgacccatg cattcatcga aaaagtgatt tttcctaaag tcaaagcagg tgctttttca
     1981 catgagatgg atcataaaac aaaactacaa gaagtcttgc aaaaatcagg tgatgtatca
     2041 atcgaatacc gattgatcaa tgaagaaggt ccagctcatg aacgagtatt ttggatcgaa
     2101 gtttatgtag atgatcaatt gattggcact ggacaaggga aatcgaaaaa attagcagaa
     2161 caagctgcgg cggaaaatgc attggcagca ctgtaaaagt aagtgaggga aagctttgta
     2221 tttgaaacga attgaaatag ccggttttaa atcatttgca gacaagacag tcattgattt
     2281 tgaaaatagc gtcacagcag tggttggacc aaatggcagc ggaaaaagca atattacaga
     2341 ggctatccgc tgggtattag gagaacaatc tgctaaaagt ctgcgaggcg gaaaaatgcc
     2401 ggatatcatc tttgccggtt ccgatacccg taagcagctg aatattgcag aagtcacagt
     2461 gattttagac aatagtgatc attatctgcc attggagtac aatgagatca gtgtaacccg
     2521 ccgttatcga cggactgggg aaagcgagtt tttcatcaat aaacaaagct gcagattaaa
     2581 agatatccaa gaattgttct tagattctgg attaggaaaa gaatcgttct caatcatttc
     2641 tcaaggaaaa gtagaagcga ttttcagtag taaaccggaa gaccgtcgtg ggatctttga
     2701 agaagcagct ggtgtcttga aatacaagca gcggaaaaag aaagcagaac agaagctttt
     2761 tgaaacagaa gacaacctca gccgcgtcca agacatcatc cacgaattgg aagaacagtt
     2821 aactcctttg gccgcccaga gtgaggcagc aaaggagttt ttacggctaa aagaaacact
     2881 gactcaaacc gatgtctctt tgatggttgc tgaaatcaag acagcaaaaa aagattggga
     2941 caacaaacaa gcacagcttg cgaaattcaa tctagaatta ggcaaactat ctgagtcgat
     3001 ccaagaacaa gaatccattt tggcgaagca gcgtaaagaa aatgcgcaag ccgatcgtct
     3061 gatcgaaaaa aatcagcaag tattattaga tctttcggaa aaactgaagc agacagaagg
     3121 acaaaaagat gttttgcagg agcggacaaa gcatacacaa aaaagctcgc aagaatatca
     3181 aacttcatta gcagaagcac aaaaaaaggt taaacatttt gaaaagcttc aagaaagctt
     3241 gatgaaagca gcagcagaaa aagaaacaga gatccaaaaa gctgaagcaa atctcataaa
     3301 aacacagcaa gaattagaaa aataccaaaa atcaacgaaa gaattattgg cagaattaag
     3361 agatcaatat gttgatttga tgcaagaaca agcagctgta ggaaacgaac tgaagtatct
     3421 agaacgtcaa tatatacaag aaacagcaaa aagcaagcag acgttagcca agcaatcgga
     3481 agtagaagca tcagtagacc gattgatctt acaaaaagaa gaattgacac aaaagcaggc
     3541 gcagctaaaa tcatctttaa ctgaaacaaa agagaagtta gaaatgatcc aacaaaacgg
     3601 gaaaaagttc caagaagcat tagcaaaaga acagcctaaa atgtatcaat taatgaatca
     3661 agtccagcag ctacgtgctc gtaagaaaag ccttcaagaa atccaagaaa actattttgg
     3721 tttttaccaa ggggttcgat tggtcttgca gcataaacag cagctttcgg gtattgtcgg
     3781 agcagtggct gaactgatcg atgttcctgc agatttcacg ttagctatcg aagcagcttt
     3841 aggtggggca gcccagcatg tcatcgttga aaatgaaaaa gatgctcggc aagccatcac
     3901 gtatttgaaa cagcaaagag gaggaagagc aacattcctt ccactgacga caatcaaacc
     3961 aagacaatta ccggctcata tattgacaca agcagcagct gtagaagggt ttatagggat
     4021 tgccagtgaa caagtcagtt atcccgatca aatccaaact gtggttcaaa acctgctagg
     4081 aacgatcctt ttagccaaag acttaactag tgccaatgca atcgcgcaaa cgatccgcta
     4141 tcaatatcga gtggtttcct tagaaggtga tgtgatgaat gccggaggat ccatgacagg
     4201 tggagcgaat aaacgaggaa accaaggcag tttgtttgta caaaatcaag aattgaaaca
     4261 gttgactagt gaatttgaag aagcagataa acaactccaa gcacaagaaa aaaaagttca
     4321 ggagctgcaa caagaaacag ctcgtttagc cgaagaacaa gaagttttgc gtacacgagg
     4381 cgaacaatta cgttttgaag aacaagaagc gacaaatcag ctacaaaaca tcataaatga
     4441 acttgaacgt tttgaaaaag aaaagcaaat ctcaactttt gagacacgtg aactgcagca
     4501 gtttatcgaa gattacgaaa agcaaacgaa cgaattgaaa gacaaacaaa cagatcttga
     4561 atcgcaacga caaaaaatcg acgaagaaat caaatcgtta agtcaagaaa gcgaccagat
     4621 ggaagccaga agagcacaag tacaaagtca aaaagcacaa gaacaagcag atcttgctgt
     4681 cttgaaagag cagtttaatc atctgcagat ccagctccgc ggtgcccgag tccaaaaagc
     4741 agaggcaacc gaacgtcaag aagcgataga aaagcagttg gcgacattga cagcagactt
     4801 ttctgatcat gaagtcacag aagaaagttt ggaaaaacaa attaacgaat tatctgctca
     4861 gcgagaaaca ttaaaagccg agttggcaaa agccaaagag cagcgagata gaaggcaaaa
     4921 ggagatcgat aagcttgaag cagtcttggc agagcgtaat cgtgagcaaa aagcacgact
     4981 gtcggaacaa tccaaattag aagtccaaaa agaccgagct gaaatgattt tagataatca
     5041 tttgacctat ctgcagtcag aatatcagct gacatttgag aaagcttccc aagactatca
     5101 agaaacaacc gatatcgaag acagtagaac aaaggtttca tccttaaaag aacagattga
     5161 aaaactggga ccagtaaact taaattcaat cgaacaatac gaacaagtca gtgaaagaca
     5221 tacattctta gcaacacagc gagatgatct gttagcagcg aaaaaccaat tgtttgaaac
     5281 gatggatgag atggatgatg aagtgcggac aagattcaaa gaagtatttg aagctatccg
     5341 tcaagaattc aaaattgtct ttccaaatat gtttggcggc ggtaaagctg aattagtact
     5401 gacggaacca actgatttgc tgaaaacagg tattgagatt gaagtacaac ctcctggcaa
     5461 aaaactgcaa agtctaagtt tgctttctgg aggagaacga gcacttacag ccatcgcgtt
     5521 gctcttttcc attatccgtg tctgtcctgt tccgttttgt atcttagatg aggtagaagc
     5581 agcgttagat gaagccaacg tgaaacgttt tggtcgttac ttgagcgatt tccaagatga
     5641 cacacaattt atcgtagtca cacaccgcaa aggaacgatg gtcgcagcag atgtactgta
     5701 cggagtgacg atgcaggaat ccggtgtatc aaaaatcgtt tctgtacgga tggaagatat
     5761 caatgaagag gcgaaaatca acgaaacgaa agtgtaggaa cgaaaatgat caaattagta
     5821 gcaatcgatt tggacggtac attattaaat gaacaaaagc tgatttcaga tgagaataaa
     5881 caagcattag cacaagcaaa acagcaaggg gtcaagatcg ttctatgtac aggacgcccc
     5941 ttagctgcga tggcgcatta tctgcaagaa ttaggactgg tagatgaagg tgactacagc
     6001 atcactttca acggcggttt ggtacaaaaa aatgatactg gtgaaatcat ggaaaagaaa
     6061 gtgatggaag tatcagatat ccaacgctta tacaaactgg ctcagcaatt ggatctgcca
     6121 ctagatgttt tgtctgacaa tgtggtttta cagctgccta gtgccccaag gaagcaatcc
     6181 ttgtacaatg tgttgaataa gctgctgcaa ttccaaccag cggatataag cgatattaca
     6241 gatgaatttg tgcttaataa ggcagtaatc gcttatgacc aagaagaatt agatcctaaa
     6301 atcaaagaaa taccagcaga attccatgaa atgtatgaga tcatcaaaac aagaagtatg
     6361 cttttggaat ttatgccaaa aggtgttaca aaagcttatg gtatttcttt attggcaaaa
     6421 gatcttggtt tgaaacaaga agaaatcatg gcgatcggtg atgaggaaaa tgatttaccg
     6481 atgatccaat atgctggact aggcgtagcg atggagaatg ctgtgccttt cgtcaaagaa
     6541 gcagcagatt tcgtgacagc cagcaatgaa gaaaatggcg tagccaaagt gatccaaaaa
     6601 tttgtattag agtaggggga gtcaaatggg attttttgat aaaatcaagc gtgcttttac
     6661 tggcgaagag aaaacagaag aagttgaagt acaggagaag tacgacaagg gtttagaaaa
     6721 gacgcgtaaa acatttggtg aacggatgaa tgagttgttt gccaattttc gaacagtcga
     6781 tgaagacttt tttgaagaat tagaagaaac acttatcggt gcagatgtag gttttgaaac
     6841 agcgatacga atcaccgaag cattacgtca agaagtgaaa ctcagaaatg cgaaaaaatc
     6901 ggcagaagtc caaaatgcga tcatcgagaa acttgtggat ctctatgaag cggaaggaat
     6961 caatgaagta aatgaactaa atatccaaga agacggacta acagttattt tgtttgtcgg
     7021 tgttaatggt gtagggaaaa caacaagtat tggtaaactt gcccatcagt tcaaaaacga
     7081 aggtaaaaaa gtattgatgg cagcagcaga taccttccgc gctggagcaa tcgaccagtt
     7141 agttgtctgg ggcgaacgcg ctggtgttga agttgttcgt ggcaatgcag gaggcgatcc
     7201 agcagctgtc gtattcgatg ctttggaaag agcaaagaca gagcaggcag atgttttact
     7261 agttgatact gcaggccgac tgcaaaataa agtgaacttg atgaacgaat tagaaaaaat
     7321 aaaacgagta atccagcggg aattaccaga agcaccccaa gaaacacttc ttgtattaga
     7381 tgcaaccact ggtcaaaacg cgatggttca agcaaaacaa ttcaaagaaa caacagatgt
     7441 gaccggtctt gttttgacaa aattagatgg tacagcaaag ggcggtatcg tacttgctat
     7501 ccgtaatgaa ttgcatttac cagtgaaatt ggtcggtctt ggtgaaggaa tcgatgactt
     7561 ggagccgttt gatccaaatg attttgtcgt tggattgttc aaaggattac taaaagaaga
     7621 ataacccatg tctaaaaagc aggagctgtg acaaaagttg cagctccttg tttgaatgat
     7681 tgtttttaac atagctgtgt ttttgatgcc ttcattttta tacttatttg taagcggttc
     7741 tcaaacaatt tcgttctttt tcttagactt tttgattata attagattca tatgaattgg
     7801 aggtcactta atgatgaatt ttaagcaatt attattgcat gtcaatgcgc gcccttttat
     7861 cgaccaagct cgatttggta tagaacggga aggacagcgg gttgatcttg caggaaatct
     7921 agcaaaaacc gatcatccag caatctttgg cgatcgatcc tatcacccct atatccaaac
     7981 agattttagc gaaacacaaa cagagatgat cacccctgtt accgattcta ttcccgaatt
     8041 atttcagtat ctcgctgctg tttatgatgt gactgctcgt tctataccga aagaagagat
     8101 gatctggcca ttaagtatgc cacctgcctt accggaaaaa gacgaagaga tcattattgc
     8161 aaaattaaaa aatttcgaag atgtcttgta tcgacgttat ttagcaaaaa aatacgggaa
     8221 gcgaaaacag atggtaagcg gtattcattt caattttgaa tttggcgatg agttgctaag
     8281 aacattgttc agccatcagg aagaatttca agatttttcc gaattcaaaa cagaacttta
     8341 tttgaaaaca gcgagaaact ttatgcgcta tcgctggatg atcacttatt tattcggtgc
     8401 ttcaccaatg agtgagaaga actacttttt agatgaatca catccgcaag aacctgttcg
     8461 cagtattcgc aacagtgcgc tgggctatac gaatcatcca aatgtgaaag tttcctatgc
     8521 ttctatgaaa cagtatttag cagatatcga gcgaatgatc gaagaaggga aactttcgga
     8581 ggagaaagaa ttttatacac cgcttcgttt ccgaggcgga aagaaagtcg cagatttagc
     8641 aacaacaggt gttcgttata ttgaattgcg gaatatcgat ttgaatcctt atgcaagatt
     8701 gggtatcaat ccagagcaag ttcggttctt acaattattc ctgatgtaca tgttatggac
     8761 agaagaaaaa gaagactgtg accaatgggt agcagaaggt acgactcgaa ataacaaagt
     8821 agcactagaa caaccatcag atcaaacaga atttcatcaa gaaggtagag aaattcttga
     8881 aggaatgaag caaatgctgg ttgaattgga ttggttggat tctctttatt tagtagagga
     8941 agcattgact caaatggatc atcctgaaca aacgttagca gcgaaactct atcaagaagc
     9001 acagctatcg agtcagcaag aagttgctgt ggcattggga catcaatatt ataaagaaag
     9061 ccatgaacgt ccctatcagt tggccggttt tcgcgaaatg gaactttcta ctcagatatt
     9121 tatgttcgat gccatccaaa aaggtgtcca agtcaaagta ttagatgagt cggatcaatt
     9181 tttgcgtctg caattccaag accatgtaga atatgtgaaa aatgcaaaca tgacaagtaa
     9241 ggacagctat atcgtcccac ttatcatgga aaacaaaaca gtaacgaaaa aagtcttaaa
     9301 agaagctggg ttccgggtac caggaggcgc agaattttca tccatggaag aagcggtaaa
     9361 agcttatccg agatttgccg agcaggcatt tgtgatcaaa cctaaatcaa ccaattacgg
     9421 gttaggtatc acgattttca aggaaggagc tagtctggaa gattatcaag caggtttagc
     9481 cattgctttt cgtgaagaca gttcggtttt agtagaagaa tttatgccgg gaacggaata
     9541 tcgtttcttt gtgatcgacg gagaagtcca agccatcatg ttgcgagtcc ctgctaatgt
     9601 tattggtgac agtatccgaa cagtgaaaga acttgttgaa gaaaaaaata gtgacccttt
     9661 gcgtggaacg aatcatcgtg cacctttaga attgatccaa ttaggtgaat tagaacaatt
     9721 gatgttgaaa gaacaagggt tgacgatcga atcagttccc caagccaatc aaattgttta
     9781 tctaagggag aactcaaata tcagtacagg tggtgattcg atcgatatga ctgatgaatt
     9841 ttctgaagct tacaaaaaaa tcgccgtttc tgcggtagaa gcactaggag ctaagattag
     9901 tggtatcgat ttgattatac cagataaaga aatcgatcct acaactgata aaaaagcata
     9961 tgggatcatc gaagcaaatt tcaatccagc catgcatatg catgtttatc cttttgcagg
    10021 aaaaggcaga agattgacga tgaatgtatt gaaattgtta tatcctgaag tattttaaaa
    10081 gaaaaagctt ttgacagatt gtcctatttg atgtcaatct gatcaaaagc tttttttgca
    10141 tgtaaaaatt tatgtgtttt ttcgttttaa aataaacgat cccagaagct ttttttcttt
    10201 tttggttttt ctttttcagc tggcgaagag ttttcttctg ggtatttttt ttcgatatca
    10261 atcacaggtt catttactgc ttcttttgct gttaagagca atccgaattc ctcaggattt
    10321 gcagtgacgt aatcgttgac tacagtgaaa cggctattgt ttttcgttgc tagctggata
    10381 tattggtttt ggacagaaag gggtagagcc ccattcaaga gtatggaagc atctggatgt
    10441 tttttgagtt cttttaaaaa gttttctttg ttttttggtt ggatcatttc agcaacagtc
    10501 atactaagat agcagcgttc cctgaatgtc cctaaatatt tatgctgttc ttcagggttc
    10561 actaaaggtg taccataaag tcccttatcc aaatggcttt ttacatcatc ttgtgccata
    10621 aaaaataccc tccttcttct ctttgtcttt cttacgatta tagcatattc ccaataatac
    10681 ctaaaacata tgaaaaagcg gttgcttttg ttcaacgatt cacttaaaat aagaatgaaa
    10741 atcattctca attagaaagg tggattggat gcgctacgga cgactagtag cattattagt
    10801 tattttaagt agtatttcat tgttcatcgg agtatcgaat gtacaattgt cagaactgct
    10861 ccagttagat gggcaaagct tacaaatctt gctagtgact cgtttgccac gaacgatttg
    10921 tttgatcctt gtgggagcga ccagtagtat ttgtgggttg atcatgcaac atttgaccca
    10981 aaataaattt gtttcaccaa cgactgcggg cacgatggat agtgcgcgtt taggtatttt
    11041 agtggctatg attttcctac ctggtgcatc cttgattgtc cgatccttga cagcattttg
    11101 ctttgctttt gcaggaacat tgcttttttt atcccttacg cgattgttcc tccagaaaaa
    11161 tcaattgatg cttccgttga ttggagtcat gttagggaat atcattggtt cagttgctac
    11221 tttttttgct tatcaattcc agttggtaca aaatatgact tcatggcttc aagggaattt
    11281 tgcgactgtg atacgaggaa attatgagtt attgtatgct gttgttccat tgttgctctt
    11341 ggtttggcta tttgcttacc gatttacgat cattggtctg ggcgaatcgt ttgcagtaaa
    11401 tctgggaata gattaccaga agatgcagtt tttaggcatt ggcttagtat cattggctag
    11461 tgcagtcatg ttgatcatgg taggaagcat tccttttatc ggagtgatca ttccgaatct
    11521 tgtctctaga atatatggag atcaagtgca caaaacaatt ggcgtaactg ctttagcagg
    11581 tagtttgttc ttagtatttt gcgatcttgt cgctcgcgtg ttgatttttc cttatgaaat
    11641 accagtcagt gtgatcgttg gaatcatcgg tggggctatc ttcctttatc ttctgataag
    11701 gggacgacga tatgcttaaa aaaaagccat ctatttttat tggtatgctt gttcttgttt
    11761 tatttgttac aggaatgtac ttgacgatcc atacatatgg aaactggcat tttgcatgtg
    11821 cattgaggag taagaaaatt tttgcattta tccttgtctc gctagctgtc agcagtgcca
    11881 caatcagctt tcagacaatt acgcataacc agtttttgac tccttcagta ttgggacttg
    11941 agtcgttata tgtccttgtc caaacggtcc ttttcttctt tatcggagga gtgacgatgc
    12001 ttcagcagac aggtacagtt tttttcttat tgaacgttgg gctgatggtc gggctgagtc
    12061 tattgttatt tttctttctt ttgaaaaaaa tgagagcgaa tcttttttta ttactgatgt
    12121 cagggatgat tttaggtacg ctttttggta gtgtcagtac ctttttacaa gtcatcatgg
    12181 atccgaatga atatgatttg cttcaaggca gattatttgc aagctttggc aatatccaga
    12241 gtcagtatct tttgcctgct tctttgatca ttatcggctg ctggtacctg ttatttcgat
    12301 atcgacatgt gctagacgtt ttccatctcg gaaatgaaca agcagtaaac ctcgggatag
    12361 acacagatcg ttttgaattt ttgattttat gttgtgtcag tcttttgaca gggacatcaa
    12421 cggctcttgt gggtccagtt acttttttag ggtttattgt cgcaaatatc agttatcgtg
    12481 tcttttctac ttatcgtcat ggtcagctat ttatcggaag ctttctggta gctatgctgt
    12541 ttctgacagg aggacagctc gcagtagagc agattttcgg ctggaataca acagtgagtg
    12601 tcgtcgttga atttgtcgga ggaatttatt ttgtcacaaa attgattcgc gaaaggaaga
    12661 gtatcctatg attgatttac aaaaggtcaa taaactgtac catggaaaat cagtggttaa
    12721 agatgtacag ttaacgattc ctgaaaagaa gttgactgct tttatcggtc caaatggtgc
    12781 tggaaagtca acggtgttgt cgatgatcag tcgtttgata ccaaaagata caggtgaaat
    12841 ctatttagac cataacgaag taaaggcttg gaaatccaat gaattagcca aaaaattatc
    12901 gatcctacga caatcgaatg aaataaatgt ccaaatcacc gtgaaagaat tagtagaatt
    12961 tggacgattt ccttatacga aaggaaaatt gacgacaaaa gacgaggaaa tcgttcagga
    13021 atcactggaa caccttgggt tagaagaaat agcggatcaa tcgattgaca ctctttcagg
    13081 cggtcaactc caacgtgcgt atattgcaat ggtgctagcg caagacacgg attatatttt
    13141 actggatgaa cctttgaaca atctagatat gaattttgcg gttcaaatca tgcagatttt
    13201 aaaaaattta gtacaagaaa tcgggaaaac aattgttatt gttcttcatg atatcaactt
    13261 tgctgcaagt tatgcagacg aaattgttgc aatgaaagat ggacagttat ttacgcaagg
    13321 aacgacccaa gaaatgataa aaaaagatgt attggatgaa ttatacgaaa tggacatacg
    13381 gatttgtgag attgaaggcc gcagattctg tctttatttc taaaactaac attaggagag
    13441 aagagatgaa aaaattagta gtaggtatct tgattctagc atcatttggt ttagcagcat
    13501 gtggaaatag caacaacacc agtcaggcag acacaaaaaa aagcagcaca caaactgaaa
    13561 ctacattgac gataacagat agtaatggag accaaatcga ggtgccaaat aatccagaaa
    13621 aagtcgtcgt attcgacaat ggttctttag atacgatgga tgctcttggt gttggagata
    13681 aggtagttgg agcagctaca agcagccttc ctgagtatct ttcatcctat aaaaaagtag
    13741 aatcagcagg tggaataaaa gaaccagact tagaaaaaat taatcagctt cagccagatc
    13801 taatcatcat ttctggccga caaagagatt ttcaaaaaga tctaagtcaa attgctccga
    13861 ccattttcct ttctttagat gcgaaaaatc catgggaatc tttccagcaa aatgtcactg
    13921 ctttagggga gatctttggt aagcaagaag aagctaaaac tcaattagag gaattatcta
    13981 gtgcaatcga tcagacgaaa aagaaagcag aagctactga caaaaaagca ctagtcacac
    14041 ttgtgaatga agggcaatta tcagcctacg gatctggttc tagattcggt ttcatccatg
    14101 atttatttgg ctttgaacaa gcagatgatc aaatcgaagc atccactcac ggtcaaagtg
    14161 tttcttatga atatgtgctg gaaaaaaatc cggatatctt atttgtagta gacagaacaa
    14221 aagctattgg tggagacgac agcaaagatg atatttctgc caatgaactg gtagcacaaa
    14281 cgaatgctgg taaaaatcag cagatcatct cccttgaacc agatgtctgg tatttaagcg
    14341 ggggcggttt agaatcaatg aaactgatga tcgaagatgt gaatcaagca tttaaatagc
    14401 ataaataaca gaggattaaa ctctcatact atgtaacaat aatgtttctt acattcttaa
    14461 atagcaagag cctatcctca aagaagttat caaaaaccat ccgtaaaatc gtattgatga
    14521 ttttacggat ggtttttatt tatttgagaa atcttttttc aacgtgaatt cattatctac
    14581 atcatacggg ttgattctag aatggggagt ggcaaaaaat agtgtgatcc attttttcag
    14641 catcaagatc gcctcctttg atttgaatca aaggtacaaa gcgaaactta gataaaactt
    14701 aaatttagat atttttattt tgtaccgatc tcttctttaa acgatgttcc atctgctgct
    14761 tctcggcaat aagtccacta gactagaaaa tatgagaagc tattttctgt ccagcgttct
    14821 tattactcaa aacaaatcgc agttgtcaca atctcttcta aacgatgttc catctgctgc
    14881 ttctcggcaa taagcccact agactagaaa atatgagaag ctattttctg tccagcgttc
    14941 ttattactca aagcaaatcg cagttgtcac aatctcttct aaacaatgtt caatcagctg
    15001 accttcggta ttagctcaaa gcactgattt cacaacctct atttaggcat gtgtctcagc
    15061 aagcggttga tagttgatga tctgcatttt tcgataaaaa tcggcgggaa gcttactcca
    15121 ccgttttagt aattgataga aataactctc ttctgctttg attttatact cgcttaatat
    15181 caatccaaaa ctattcgttt tttttgaagc taatttttta taaatatcca atgctttggt
    15241 tttatcttca ttgacaaaga gtgtttttac ttcaatataa gaaccctcga tattttgtgt
    15301 ttcttcgata aaatagatgt tttttatata ttccttcata gtgattcctc caaagaagtc
    15361 ttttatttga taagtttact ctaccgaaga gttgttaata atccgtgaac aaaaagtaat
    15421 tctttttcca atagttgtta aaggtttttt attttttata gtattttttg ttatttaaga
    15481 aaggaataag gttaaacaaa taacaggttg ctttttgggt ctttttccgt tcaagaaact
    15541 gaacgaagaa aagggaaagt tgtctctttt gtgataagag aaatcataat aaacgaatga
    15601 cttactttcc aataaattga gaacagtcag aaccagacaa attagttgca tctgatgaat
    15661 aaagtgatta ttctatgaca tgaggtgata cagatgaaat tagaagtgac aaaagaagca
    15721 caagaagtat tgaagaataa attagaagca gacgatcaac ttttattaga tatcgaaaac
    15781 ggtgacggtc catttgcaga aagccaagtc agttgccaat tggatacggc atttcgattg
    15841 atcattgtca aaaaagatac acaaacagat ctaagtcttt attctgtagt ggctgataca
    15901 ccagttggac caatcaaaat caaagacagt gcaatacttt acatggatga tccatccaca
    15961 cttgctttag aaccaactta taatagttta caattaaaag gaccaagcgg cttgagaaaa
    16021 ggcaacttgc aagttgttcg aaaagactag agggataaaa tgggaaaatt ggcgatcatc
    16081 agcgacctac atgtggacat caataaattc ggcgaatcag agctgatgca gctttggcaa
    16141 gttttgcaaa agcacaagat cactcggctt catctagccg gagatacggc taataaagta
    16201 gaccgttgcc tagctgttgt cgaatttttc gtcaataaag gattatctac gacatttcat
    16261 ttcggcaatc acgagttggc agatgtgccc aatgaacaga tgatggacca ttttccagac
    16321 ccacattttt taaatgaacg gtatattgct ttaaatgagc agaccgttct tttagggatg
    16381 aatggctggt acgactatca gttttccgaa ttgaaagatt acgaggcaat cgtacgaatg
    16441 aagcgtttgt attggtatga tcggttcatc cagcgtggga aaacagatct cgaggtcaat
    16501 gcagaaatgc ttgcagctgc aaaaaatttg ctagaccagc tggaagcaaa aaaacttgag
    16561 gtgattttat ccactcattt cgttcctaaa cgggcattta tcatgtatca atctgctcct
    16621 tatgagcgtt ggaacaaatt aaatgcattt ttaggatcag aatcattcgg agaattactt
    16681 gaccgttatt caaatatcaa acaagtagtt tttggccata cgcatcgtcg ctttgaagat
    16741 aaatccatcg gtcacacgat ttacagctgc agaccttttg gttattatta tgaatggcag
    16801 ctgacccgag attttgtctt gaaagaacag ttgctagaca gctatgatcc tatgaaatta
    16861 cgtgtcctct tgcgtgccca tcacccagct tttcaggcct atcaaacaca acatttgcaa
    16921 gaggaatttg aacaagcgat gacaatcgtt ccgtattgag acagcattag taatgtgtaa
    16981 aagaaaatac cttgtaaaac ataaggataa gtaagagcag tagatccatt tatcgtggat
    17041 atgctgcttt ttttgcggaa aataatcagc gaatgtttat ttgatcggat gatttgtaac
    17101 tcgcagagta ctagatgcaa gcgtgtattc tttttttgaa cgataatcac aaaaaaaaat
    17161 ataatataca ttttttattt aattataatt aatattgatt gaagtctagg cagtcaagtc
    17221 acattttgat agataggagg gggacttacg aattatttta aaggctatga cagctgtatt
    17281 tctagattgt gtttgcatca agttgcaata aaaactagaa atcaaatcgg gctgataatc
    17341 atttataact aatcgataca taggaccaaa tataggtcta tgttctttta tttcataagt
    17401 atcgtaaaac agaattttgc tatggattaa tccaatcgac tgatatgttt agtaaataaa
    17461 gaaagaggaa tgtcaatgat gaaaaaagaa atcgtattaa cagcagcaat gatgttattc
    17521 agcatggtag caagtacgac ttttgtttct gcaactgaag tatacccaaa ggaatataac
    17581 acagaaggaa cgattacatt tgaagcagga gatgagggtg taactcctcc tgttgatcca
    17641 gagaatcctg atcccaataa acctgtcgat ccatcagatc ctccaagccc tggaacagga
    17701 ggggcattat cgattgatta tggatcaaaa ttcaaatttg ggactcaaaa aatttctact
    17761 gcggataaaa cgtattatgc agctgctgat gtgatgaacg atgggtctag aaaaccgaca
    17821 tatgtccaag tgacagatag gagaagtacc ttatctgggt ggaaattaag tgtctctcaa
    17881 ccagaacagt ttaaaactgc cagtggggat gaactagttg gagcacagct gaaatttacg
    17941 aaagggcagg ctgtttcttt ggttgatcca acatatacgc cgcaaacggt gaattctgaa
    18001 ttaacgttga caccgggagg aaacaatacg ttagcaatta atgctaaatc aggaacaggt
    18061 gtggggactt gggtctatcg atttggtgca aatgaaaatg aaaatcaaga tgcggtacag
    18121 ctttcggtac ccggtaaatc tgtcaagtta gcgcaacaat attccactaa attagtttgg
    18181 acattagaag atacacctaa caactagttt actgaaaaga actgttaaaa ataaatacgt
    18241 agtcatattg tcttgcgagg taatcgagca aaaattgtca aaggctaggc taaatttgtt
    18301 tggatacgaa tagttcatct aggaatttaa cgatcaggag gaaaagatat attgaaaaag
    18361 aggaggatcg tgctcatttt aagcagtctc ttagtgacta atatctttcc gatcataggg
    18421 tactctttgg aattgaatca gttggagcat ggaaatagaa gagacgaaga aagcaaactc
    18481 acttcgacat tcgacagttt aatagaatcc aaccagacac aagaaagcag tgaggattta
    18541 agtatttcga gtagtggaga gaccactgac aacgtagaag ctgagggttc ggacaaacaa
    18601 ggactagaag atgcaccaat ttctgcggtt gagctgcctg acgatcccaa tattatttct
    18661 atcgataaaa tttttcaaac acctatcggt actagcacgt ccatattgga aaatgggaag
    18721 ttactgcagt tgaacccagc agaaaggtct caaagaggag ctatttggtc aaataagaag
    18781 attagtttac tctcggattt tacatttaaa tcttatttat acttaggaaa tcaacgagat
    18841 agagcgggag atggcatgac ctttacattg actggcgatc ctagaatggc tagtaatccc
    18901 agtcaagtta ttggatcgcc aggtatggga attggctcat attctactcg ttctggccaa
    18961 ccttacattg cgaatgcgct ctcgattgag tttgatacgt ataaaaatag tggttcttct
    19021 gatcgaatgg atcgagagat aactgctgat aatagagggc atggacatgt cgcttttgtc
    19081 acgcctaaac caaataataa taattattct ggagagcata ctggcgtgac ggtagctcct
    19141 acatttcttt ctaatggcac atggcgcttg ttaataatta aatgggacgc ggagcaaaga
    19201 agtcttactt atgatttgga aggtgtcggt cgctcttctc ggattatacc tgatctcatt
    19261 aggcagtttg gttctacaga agtaaattgg gggtttactt cttcaacagg tacttttttt
    19321 caagaaaatg ctgtcgctgt cacgcgactt ccatcgaatg tcacatcaac agctgatgta
    19381 aaagtcaacg atagtgatta tggggccatc gctgaagctg gaggaaacga taagataact
    19441 atcagaaata cattgaaggt cgagggggat tttcttgaat ataagaaaat gcagactagt
    19501 atcgacctcc cacaagaatt aactattccc gagcatatct tgatcaatgg aaatcaaatt
    19561 gatgccaaag atatcttgat aaccggaaat cgagtcacgc taaatctgac agattatttt
    19621 attgctggag atacgttaat aatcgaattg gagacaactt tgcaggaagt aattcctgaa
    19681 aaagtactca ctatgcattt tgaatacctt gaaaacggga atttgattga gcaatcgaat
    19741 gaaataaacc taaagatcgc aaagctgaag gagatagata tccatgttta ttataaagat
    19801 aaagacagtc agattgatat tgctgaaatg aaaacaatca ctgggaaaat aggagagaag
    19861 tataaagaaa ctcctattga tattgaaggg tatgtttttg actcggattc agcgaataca
    19921 gaaggcatct tctccgaaaa tacagaggat atctattttt attacagaaa aggcaaactt
    19981 tatctctcaa ctgctcctca atatgtcaat tttggaaaac ataaaatatc cagtagacca
    20041 ttaagtgaat tcggacagtt ttcaggtggg ttgaaaatta tagatgaacg ggcttttgga
    20101 agttggaaat tacaattgaa acaagagaaa gttcttacaa atggagattt tgagcttcca
    20161 gaagctcttt cctttgttac ctctgaaaat acaacggtta tagggacatc cgctgttacg
    20221 attttcgaaa gtgatcaaaa gggtgaaagc gatttgtcct ctcttttaga ttctacagct
    20281 catcgtggaa tcagaataaa tattcctgta gaaaaccaaa gaatcggaac ctttgagggg
    20341 aaactatctt ggatacttgc agatgtccca ggaaattaac aggaagagaa gaatataaca
    20401 aaattattga gtttcaagaa gggcacaggt accataggct cttgtgtccc acagagacaa
    20461 gattttgtta tagtcttctc ttacatatga ttaaaggagc gattttcttg agaccaaaac
    20521 aacaattatt tataacactc ctagttattt taggggcact ttttaacaat atatcagtgt
    20581 cagctagtgc atttaatttt tcagtgacgc cgataacttc agagaatcaa atcgacaaga
    20641 gaaaaactta cttcgatctt caacttgatc cggatcagga agtagaagta aaagcggaat
    20701 tgagaaatga tacagaaaaa gaagtgaaaa tcgatatttc tgtgaatagc gcaaccacca
    20761 attcaaatgt gatggttgaa tacggaaaaa acgagattga aaaagacatg agcctgatct
    20821 ttgatctggt agattatgtg agctatccac agatagtgac gctaaaacct aaaagtgtcc
    20881 aaaccgttgc ttttcatgtc aggatgccga atgagcgatt tgatggtgta ttggctggtg
    20941 ggatcacatt ccaagaaatc cagacggaaa aggaccaaac agaaactaag gatcaaagct
    21001 tatcgattga gaatgaatac acctatatta ttgctttgtt gatgcaacag acactgaacg
    21061 aagttgctcc gaaccttact cttcatgaag ttaaaccaga tcaaatcaat gcaagaaatg
    21121 tcatcttagc aaacgttcaa aatgatcaaa aaacctatat caatcaagta gttattgaaa
    21181 caaaaattac aaaaaaagga cattctgaag ttttatatca agaggaaaaa gaaggattgc
    21241 agattgcgcc gaatacaaat ttttcttttc caactgcttt aaatggtcaa ccgctaaccc
    21301 caggtgaata tcaccttaca atgactgttt taggaaatga aaatgaaaat ggaaaatttt
    21361 cgagaaaaaa aggaaatact acgatcaact ataccaatca atgggtattt gaaaaagatt
    21421 ttacaataga cggaaaagta gccaaggaat tgaacacgac ggatgttacg attaaaaaag
    21481 acaataattg gatttatatc ttagtagctt cacttttctt attgtttatt ttattatgca
    21541 tacttttgtt tgttttgaga aaaaaagaag agaaagaaaa gtaaggttaa gataacaaat
    21601 agtaaatttt tattattgta ggatggttgg gctcttttta taacaatata aaatatacca
    21661 ttttattaat aaaaatgcat actagtaaat gatataattc taataacagg tttgatcgcg
    21721 agaaaggcca aataaaacgc tctccaaaat gttaaaaaaa agacaggacg atgtatatta
    21781 tacaaacaag aatcgctgac aacagtgact cggatccagg aaatcagttt tatccccagc
    21841 tagttggtga atgcatggaa agtaatatac gtagaaagag ctgatgacta atgaattatg
    21901 agtttttttt aagcaaagaa gatcacagaa gttattcatt acttaaatat ttagaagcaa
    21961 gctcaaattt atcagagtca atatcgaatg ttcaagaaga gttgtcgcta tcgacatttc
    22021 tcttgaaaaa aacgatcgat aagctgaaac atgatttaca aaagttcgat ctagagaaaa
    22081 atttgcgagt aaccgtcagt gaattggata tatgtctgga aattaatgga aaatgttcta
    22141 gcaaagctct gttatctcgt tatataacgg attctttatc tatgaaaatg gtattggctt
    22201 tctttcagga aaaatatatc tcagtagaaa attttgctaa acagcatcac gtgagttatt
    22261 ctgtggctta taaagtatta caaagactaa aaagaaatct aaaaaaatat caaatttttt
    22321 ttgaaaagag aaagtt
//
LOCUS       DANKHL010000026        22258 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000026 DANKHL010000000
VERSION     DANKHL010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.028"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(13..555)
                     /locus_tag="PW101_000691"
     CDS             complement(13..555)
                     /locus_tag="PW101_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513117.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(576..1667)
                     /locus_tag="PW101_000692"
     CDS             complement(576..1667)
                     /locus_tag="PW101_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL2513118.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(1675..3885)
                     /locus_tag="PW101_000693"
     CDS             complement(1675..3885)
                     /locus_tag="PW101_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513119.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(3901..6429)
                     /locus_tag="PW101_000694"
     CDS             complement(3901..6429)
                     /locus_tag="PW101_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2513120.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(6481..6891)
                     /locus_tag="PW101_000695"
     CDS             complement(6481..6891)
                     /locus_tag="PW101_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513121.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(6904..7122)
                     /locus_tag="PW101_000696"
     CDS             complement(6904..7122)
                     /locus_tag="PW101_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513122.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(7119..8111)
                     /locus_tag="PW101_000697"
     CDS             complement(7119..8111)
                     /locus_tag="PW101_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2513123.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(8190..9101)
                     /locus_tag="PW101_000698"
     CDS             complement(8190..9101)
                     /locus_tag="PW101_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2513124.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(9120..9464)
                     /locus_tag="PW101_000699"
     CDS             complement(9120..9464)
                     /locus_tag="PW101_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513125.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(9484..10040)
                     /locus_tag="PW101_000700"
                     /pseudo
     CDS             complement(9484..10040)
                     /locus_tag="PW101_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10121..10621)
                     /locus_tag="PW101_000701"
     CDS             complement(10121..10621)
                     /locus_tag="PW101_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513126.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(10622..11167)
                     /locus_tag="PW101_000702"
     CDS             complement(10622..11167)
                     /locus_tag="PW101_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513127.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(11164..12420)
                     /locus_tag="PW101_000703"
     CDS             complement(11164..12420)
                     /locus_tag="PW101_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL2513128.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(12781..13092)
                     /locus_tag="PW101_000704"
     CDS             complement(12781..13092)
                     /locus_tag="PW101_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513129.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(13115..14425)
                     /locus_tag="PW101_000705"
     CDS             complement(13115..14425)
                     /locus_tag="PW101_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HDL2513130.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(15039..15491)
                     /locus_tag="PW101_000706"
     CDS             complement(15039..15491)
                     /locus_tag="PW101_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513131.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(15493..15858)
                     /locus_tag="PW101_000707"
     CDS             complement(15493..15858)
                     /locus_tag="PW101_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513132.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(15957..20240)
                     /locus_tag="PW101_000708"
     CDS             complement(15957..20240)
                     /locus_tag="PW101_000708"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HDL2513133.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(20387..20626)
                     /locus_tag="PW101_000709"
     CDS             complement(20387..20626)
                     /locus_tag="PW101_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513134.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(20716..20988)
                     /locus_tag="PW101_000710"
     CDS             complement(20716..20988)
                     /locus_tag="PW101_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513135.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(21071..21322)
                     /locus_tag="PW101_000711"
     CDS             complement(21071..21322)
                     /locus_tag="PW101_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513136.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
BASE COUNT         6519 a         4830 c         3314 g         7595 t
ORIGIN      
        1 tcatttttta ttttaatacg cactccgtac ataataaatc ttgatcgcat caatcttttg
       61 agcttcttga tctactttca attgaagtaa tacaaattga gtaaccttat tcattgtttc
      121 ttctccaaat aacaaatatt gatccggatt ctctatatct tgtgtcacct ggtaaagttt
      181 cccagttgtt ttcatttgta catgcgggtc tacatcaata ctattgtctt taatacaacg
      241 atctgtcata tagtccctca cagcttgatt gcgtttataa acatctgtga aattactcca
      301 ttttctagca aaatctttta agaaattttt tgtcatttca tctatttgca tttgttgcgc
      361 tgtctcgtct tctaatgaac caatttgact gttactttca gtactttgaa gagttccact
      421 tgtttctttt actgcttcga ttgtttcttc tgtcgttctc aattgtgttt tttgtttttc
      481 agaagaaaac caatgacaac cagtaagacc tatcaaacat aaccaactaa atactaaaat
      541 tatccctttt ttcacgtttc tagtcactgc ctttcttatt tcaccttacg aaccagacta
      601 cgaatttttg tttgattgta cgtgcgttga taccgagcca taatccgtcc ttgctcggca
      661 ttctgttcca cactagagaa atgttgtccg ccatatgaaa catctgttat aattccggtg
      721 tgaccataga ttccaggaga caatgcacca cctgcttgcc aattcaccac atctcctgct
      781 tgtaaatcac ttggttttgg gttcatgatt acttgccaac cataggttgt ccagttataa
      841 tcttccccta ttaacatcgc aaacatgtgt ccagaaccca ttaactgtgg gccacctaat
      901 tgagatacat accatgccgt accaccataa cattccccat tatttacgga tcgtccaatg
      961 atccgatcca aaaccttcgc attgccttta ctttttcctg caataagcga agtttttttt
     1021 gttggtaggc tattccaatg cttgatcgca aaagtatcat attccgttaa gttataggac
     1081 gttatgatac tcattgctcc tcgataatac ggactccctt cggtaatacc tgctgccgca
     1141 tagcctccac gacctagaat caataaacaa cttccaggat ctttattgcc cacacattga
     1201 ttaaagttgc tttggctcgc caccataaag ccataatagt cataacaagc ttttaagttc
     1261 ggaaacttca tatagaaacc accttcagaa ccaccaattc cacgtgcaga accttgtggg
     1321 tagggacaac caggaaacca ggtaatccca aaaaagttcg tatcgtttct tccagattgt
     1381 gactgtccat aagctgattc atagcctaat tgagaaagaa tcacactcgg taataactga
     1441 tactttacag ctgcaccaac tgctgcttgc gcatgttcat ctgtgattgg accacgtcct
     1501 ttaatctctg aaaaaatcgg taatccttcg gtatattccc cattaaaagc gacagctaga
     1561 cctgtagctt ctcctccatg ctcagaaagt tctcctgaaa aaaagaatgg caatagaaaa
     1621 aagggaagaa taaagaaaag aagacaacct agacaccctt tcttcatttc ttcctcatcc
     1681 tttcttcgat aatggttttt ggactgttcg tccttttggt aaagtaaaat cagttcctgg
     1741 ttttgcgtcc aaaatcggca gtgttttagg atccttttct attttttccg attgctctgt
     1801 tggcttatct aaagaagaaa atgcaggttg taacgatttt ggtgcggaaa tcgacggttt
     1861 actttcagac gaacgttgcc ccggttgttg cacagaacga tttcctgata gagtggctac
     1921 cgacgaatta ggaacagagt attggttcaa taactgctgc attgctggat aactcccatt
     1981 tttcgcctga tttttcactt ggcgaatctg ttgtgaaaga gaaccagcag ctgttaattg
     2041 tggcacgggt acttttccag aaggcaacgg aatcgattga ccaacaaaag cgataccttt
     2101 atttaacaat ggttttcttt gattattccc ttgttgctta ctcaattgtg ccaatgattg
     2161 tacctgttgt tttggtctta ctgaattcat ggacgaattt ggaatcaaac catttgaaag
     2221 accagaattc cactctgttt tcgatttggt agaagacatt tcaggctcta ccccatgata
     2281 acgaacatga cgaccactat aaggcaaggc ttgatttgaa cgcccaccta gttcaaaggg
     2341 attaaccact tgcctccctt ttaccgattc ttgtggtgta cgtttttgtt gaaacttttg
     2401 aaatccatgt atcgctgcag aagcgagtgg attcgttgct cgatacacat tccctgctaa
     2461 tcctgcacct ttgattgcca aattttgtcc agcagttgat agttgaccag gcagttgttt
     2521 catttgagac aaagaatgac ttgctatgtt tttcgtgttg tttatagttt gatctgcagc
     2581 tcctaaccct agtttcttta ataaatcttt ccgttttaca atgagtaaaa ttgataggag
     2641 tgctttgaca aacatatttc ctaaaaaacc aaaaacagta ttcattggaa ttaaatcgtc
     2701 tactatgtta tagatcaaaa atgccaccaa aataatcaca ctgtaaaaag atttttgaaa
     2761 catcaagccc aaaaaggact ttaaggtatt catgagcata taatcaaata ctggaacaaa
     2821 agaagcgagt aatgaaaaag gaatcacaag taataaacac aacaccaaca tttgaaagat
     2881 aaaacgaatc atgccaatca ctacaagtgg taatccaata cttagattaa gcaatggcac
     2941 catagaacca acgactgctt tataaacagc tttagttggt tgaatataga cacgataagc
     3001 cttctttttc cctctatctt ctcctgcagc agtggtcact tttgtctcaa tatcagataa
     3061 ttcttcactt gacgcttcct tggctagaaa ttcttgtggt tcaatctctg cttgatacaa
     3121 ttcttctaaa tttgttgtcc cataatttaa caatatatac ggatcaacaa cagccgcttt
     3181 ataataaagt tggcgcactt tttgaatggc ttccttttgt gtcgttggca ctaaaacacc
     3241 attgacatct tttgtcgcca attcatctag actatttgta gcagaaaaaa taaagccttc
     3301 tacctccacg gacaaatcat ttaacccttt ggtaaaagct tctcctttac taggtacgtt
     3361 actaccatag ccaaaccaaa ccattgaaaa aatgacaacg aagagaaatc gaataaacac
     3421 catttttgcg taatgtttcc ctctaaatat acgaagagta aatagatata acacatacaa
     3481 gacgacaatc cccgcaccaa taggatcgta tatttttgaa taaatagtat ccgaaaaagt
     3541 gaaaaactgg tgaattaact cgttcattgc agctccttca tagagcttct caatgacaaa
     3601 atcaaggcct tgccaaatca atttacttaa actaaagaac atattcgtca tcatttttag
     3661 aaaatctgtg ccatttgccc caaataacat tttgcctaat gaaaattcac tatcagataa
     3721 ataagaagaa aaggtatcaa tatgaaattt cccaatctca tagatcgatt gatccacctg
     3781 cttttctgcc gcatacacca atccaccaac aaaaagcagt ccgaatccaa tcgttaaaaa
     3841 aaacaaaacg caccatttct tttccttatg ctttatataa gccattgttt cacctcattt
     3901 ctattgcatg attaacgtat cctgttcttc tacttgagag tgacttgttt tttccgttgt
     3961 ttgtaatgca gcggtcattt cttcaaacaa actgtgtaca ttgagttttc ctacacgtcc
     4021 atagggatct aacatccaac actgtccttt aatcatgtgg ctcatttcag ctaagttcgc
     4081 ttcatttact tccaaacgta aatgattcaa aatcttttct cgctcactgt cttcatcaaa
     4141 agcaaagacg acaccaaaat tccctttttc tttatctgaa cctaagtcac caataaattg
     4201 tgtatctaac actaattgat tcatctggga acgccccaca cgtgccattg ctgcaataat
     4261 actacgtcct gcgttcgaat tattaaatac ccatgcttct gtaaaataaa tcgctgtttt
     4321 cttactacta tctcgttttc caaacatttc acaaaaacga cctaaactaa tcatcaaaca
     4381 caaagacttt cgatccgcat ctgtatatag ttctggtcgg acattgtcat ttggtaattt
     4441 caaatcttgg atctctaaaa tcgttgtttt ggcattaaag tctaatcccg ggttttctcc
     4501 ataagaaaat cctaaacgta aaatcgaatc ttctaccgtc aagcgtaaaa actcaccata
     4561 ttctcgaacc tctttttgtt ccatttgttc taaatagtcg accacatgca tcaagccaac
     4621 ttgttgtccg gcttcttttt gaactaaaac ttcttggatt ccctttaata ccgccccttt
     4681 taaaaggtga ttggtaccta acggacttaa ctcatcaatc atatcctggg ctacttgttt
     4741 cgcatctact cgggataaac aaacaattgg atctaataca ccataattac ggggatttgt
     4801 agcatctaac gtgacataac tcattttttg caaatgttct acaaacaaag ggtaatgttt
     4861 ctgataatac gggtcttgaa tgagtttcgt aaaccatttg cgcttttctc ctttgggatc
     4921 cacaaataaa ctttgtacat tcataaacga actatacaaa aagagcaagc caactaaaaa
     4981 ggtcttccct ttaccggttt ttccagttac tgctacatga ggcgcatcaa acaacgcacc
     5041 tttgattcct tccgcaatgg ctagaagatt caaaaagaca aaacgatttg acgcataaat
     5101 taattcttct aatggtgttt tttctcctcg agaaccacca tcccctaatg tatcttgccg
     5161 accaatatac cagccggatt ccatacctaa ttctgatact gctgaaaaca accattcgcc
     5221 aactgcttct acagttgttc ggtgcttcca atgtaactgc cgtcccaatg gttgccctaa
     5281 taaacattta tagaacaatt ctcgttgatc attttttgca cggtggatat ggataatcgt
     5341 atgaagttgt tgaataaccg ttgcactatt ttgacgaact tcttcaaccg tttgaccgta
     5401 tacaataaaa catgcgatcc aatcgacgac aggttgccca ttatctactc gttgctttaa
     5461 atgttttact aatcctcgaa cagttaataa gcgttcacta ggttcttcat cattttgaac
     5521 caattcctta tgctctgttt tgaaccgttc cgatagccaa ttcagtttta aaccaatacc
     5581 tccctgtcct ttcaagcgtg gatagtgaat tttcattcgc aattctgctg ggaaagaaag
     5641 atcctgagca aattgaaata gatcacaatt tctcatattt tcaggtgttt ccccaatagg
     5701 caacaatgct aaataactac tccctaactc atcttctaat tgaagaaggc ctttatgttc
     5761 cttcggtata cgtacatacg ttccttgatt gtctttcagt acttcccaat tattttgttc
     5821 ttctactttg tgagccattc ctcgaatata ttgcagacga ttgagataca acagctcttt
     5881 ttttactaag ggacgaatag aaaagctata aagtaattgt gtcacttctt ttacataagg
     5941 atcactttca gaaaacaaag tttccaaatc atctacttct aatccaaata agcgtgctaa
     6001 acttcctgct aaatctaacc agattagatt tattacatca tgaaaactct ccaccgttgc
     6061 tttttctttg actttgattc ctaaaataaa tgaaggacga tagacttgac cttctcgttc
     6121 caacaatgtc actgtttctt ttgcataata cgcaccaatt gcttgactat cgggagaaaa
     6181 tccctttcct aatgtgtcaa acttcgtccg taaattcatt gttcgtggat acatcaccaa
     6241 ctccacatct tcataatctg ctaaaagcaa cagtaaacga gccatcattt cttttatttc
     6301 ctgtgtcttt tttggtgtgt tttttgaaat tgttcgtgga aaaacatgat agtaggccca
     6361 gatacaatcc tctccattta acaaacaatt tttcttgaat gcttttactg gttcttttac
     6421 aagcaccaat gaatttcacc tcccaataaa aaaggaacaa ccagttgatt gtcccttttt
     6481 ctaatcctct aaataaacat acttattcac aaaaagtaat tcttccaatg cattgtttga
     6541 taccttgtcc cagttataat acacacaatc accattcatc atacgaatca tataacaaaa
     6601 ccaatcccat aaataaaaat gcactttttt tccatcaggt tttagttttg ccagtgctaa
     6661 cacacataaa taggggagtc ctatataaaa aacaactcct gctggtggaa aaaaagaaat
     6721 gacccaacca ataaatgaac gtaaaaataa atattcaaag attacgacca acaaaaaagt
     6781 tgctgcattg actaatgcaa ctggaaatgg aagacgccaa ttgcttgtaa tctgttgtac
     6841 gcgataaggt tccgcataaa caatcgaata atcataaggt tcccaatcca cttcactatc
     6901 ctcctaatcc ataaataatt tttcaatgat tttaccaaac gcatcaaata cacgtgttgg
     6961 atcatttaag aagaaataaa ctgcggcacc tgctgcgata gatattaatg catttcctac
     7021 ttttgatttc ataaaatttc gcaatcccag ataaacaacc accattatta aaaaatagcc
     7081 accttgcgtt aatataaaat ctacaattcc cttcagtgtc atttccgttc ttctcctttc
     7141 ctatccaacc aattatgtgt taatttttca acataatact gatttgtttt tttcgtaatg
     7201 acaatcgtca tttgttcttt ttgtaaaagt gacgtatcac catctcgaaa gacgaccgtt
     7261 gcttttacaa taaaaggttt tttcgtttct ttataacgca atactttcat ctctacttga
     7321 tcaactttag ctgtttgatt gagtccttca ggttctttca tcatgtatga catatctttt
     7381 gcattgtttt tcgcatactt atctaagaaa tcttccacaa atccttcaac atctttcgct
     7441 tccgacgcat caaccgtttc gtataatgtg tcatcataag taatcattcg ttgtttttct
     7501 gtattctttg gtagtgccgt aaaataagga taagcaataa tacgacagcc cttgtttgaa
     7561 atagaaaagg gaagattgag tacttgtgtg attgtcacac ttgtttcttt cttactctct
     7621 ggattcatcg agcgaatatc ataagttact ttataactag cagttggaat ggtttcaatt
     7681 tcttttaatc caataaacga agcagcagtt aattttcgaa cacctgaaac acttcctggt
     7741 tcttgaataa agttttgtgc ataaaactct ttttttaatg tttcttgacg ttgttgttct
     7801 tgttcatgat tatcgaatgt attcatatac aaagcaacaa aacgattcat aaaggattca
     7861 atttcaggtg tgtacgttaa ttctgaccga ctttgtactt ctttcttttg taattgttgg
     7921 atttcatctt tcaaaaacga cacttgaacc ttcgttttca tggattgaaa aaatgccaag
     7981 ccccctgaaa aacaaagaaa taaaaccaaa caccaaatcc atttcactgc tttttttgaa
     8041 tggattttct tttgcttcgg tactttctct gtataaactt tttctttccg ccgtagttgt
     8101 cgttttttca taagctactc cttctagtat gtttttacta gtacgtacga ttgacaacga
     8161 taagcttgtt tttctgaaaa atagtccatt caattaccct cattttctat tccttttctc
     8221 tttcttttat atccaacaaa tccagcaata cttgtcaaca ctcctcctag aacagaaagt
     8281 gcaacatgta tttcttcacc agtctttggt aagacttttg tttttccatc tgctgttttg
     8341 atttcaatat tgccacttgg aagtttcttt acaggaatcg acaaatcctt taccaaattc
     8401 ccttcactat cttggattaa tggaatacca tcttttacac caacaattgt ttgatcacct
     8461 gctactttta atggggtggt tggtgttgga atatgggtca attcctctac attactagtg
     8521 acaatcggta cactcattcc ttgagaagta ttcgtcgtaa tttcaccgct tggggttacc
     8581 gatactgtga cttcttttgc tggaacaata ggtttatttt cttttggaat tgcttctgat
     8641 ttttcaacag gttgtttcgg ttcttctttt tgttcttcac tggtagacga catcgttggc
     8701 tgttccgtac ttgacgaatc agtattcttt tcagaagaac tggttgttcc tgattgttcc
     8761 ggagttgtga ctatacttga atccgttgtt gttgaactat gcacaccttc ttcttcgatc
     8821 gaatcagtcg gtaaagttga ttcaggaaac tctggttctg gtgtcactgg ctctttttca
     8881 ggtttctctg actgcaattg ttcaggttct gctggttttt ccgttgttcc tccatttagc
     8941 tctgtctcaa catctggtaa tccttcccct ggcaatgttt ctacaatttc tgacttaggt
     9001 gtttctacag tttcatctgc ataaaccaat gaactcccta attgaaacga aaataatgaa
     9061 attcctagtg ttgttacgac tgctaatgtt gtttttttca tgattattcc tccaattttt
     9121 tatgcaattc gtgttttgtt catagacgcc gcactaatat tatctgaacg ataaaagaac
     9181 tcccgtccgt tttcttctaa cccaacatat ggattatttt tgaataattg aatcaatagt
     9241 tcactggtgt tgtattttgg atagtttgca aaaaaataat actgtgcttg tcgcccatcc
     9301 gaaaattcaa caagtaattc ataacgatac cgcttcaatc cttttcgttg ttttaaagga
     9361 tctctatacc aacttgtcca tcgttccatt cgtggataga gatagacacg atacacatag
     9421 aacaatgcca taccacatac caccaaaaat aataatcgcc acataaaaat tccccctttt
     9481 cttctacctt tcttgaatca tcgaacattc ataccctcta tagagatttg aaaaatccat
     9541 catcaattgt tcatataaac gttgttgcaa atagcgattg tttcgaatac ataattcttc
     9601 atagccatca atcacttgat cttctttttc atagcatctt gtagctctat gacgctcagc
     9661 ttgcaagaca taaccaaact ctgcaccata tcgttctact gttaataact gttgactacg
     9721 caataatgca atataacaat cacaaaaatg ttcaaccgac atttgatttt tctcacgttt
     9781 gtgaaatagg tattctttag aaagcacatt attcgttaaa caatctgata aatagcgata
     9841 atctggcggt cggttatttt catctgtgaa tttttcccat aataaactga tttttattcg
     9901 ttcagcggct agatagtctt gttcgcgtgg ataaaattca tcaatcgcac aaagtttttt
     9961 atcacctgaa taaacaaatc catataatgc gaaacgaact cttccttttc aattggctct
    10021 cctaaaaatt ccagtgacat accatcttct ccttcctaaa aaaaataaaa aagcagaaac
    10081 cgatttggct tctacttttc tttcctttta tagctctttt ttacggaaga atccgcaaat
    10141 gacatttttt caaaaaatca atcggcacaa gttcacgaaa acttggagca attttggata
    10201 gaatcacttc cccatcttgt aaaatacaat cataaaaatc atcagttact tgatacttac
    10261 gcttcttaca ataattgata aaatagtagc gatcattttc tatttccatt cgttgttcta
    10321 ctaagcgcat acgaacatca tcatagaaat aagccacaaa tcgcccttca agttcatgtt
    10381 tttcaatggg atactcttct ggtaacaatc tattttttcg tcgtttatac attgctggat
    10441 accttagaag aagttcatct aaacgtctgt cagtatatgg taactgacga atcaacgatt
    10501 ctaatcgttc tttatacaga tagaataatt ggcgcaagca atagtgtacc gcctctggat
    10561 tttctaatac tgttggatca cgtgaccaat acgcaccaac ataaatcata atttttttca
    10621 tttagacaat cacaccagca atactaatga ccgatccaca ataggtagaa caagtggcat
    10681 agcgaccaat ttcctctaat tctatctcaa tctcttctaa agaaagatat gtctgttctt
    10741 ctgttggctg gcgactcatt tcccaagtga cagtcaccaa tgaaatttct tccataagag
    10801 gtttttcgct gctaagtgct gccacacagt tttcatatat cttcatttta tcgcagcctt
    10861 ttttaaccgc taaaatcgca ggtattggca atgaaaatcg ataaaagcga atgaattcta
    10921 gccttccttt ccttcggcta aattccaact cttcgagttc ttgttcgtat cccttacgaa
    10981 cttctaataa gcgtgcaatt tcttgccagt tctctttttc ctgataacgt ccagctttta
    11041 ccgcttgttc aatataagga actcgaaaac gaaaataaga caaatcaacc atcttttcat
    11101 ttaatgcttc gattgcagcc tttaaatcca acaggcgcag ccattctgtt gtcgtatatg
    11161 cattcatgca attttccctc ctgacgttcc catccattct agatagtttt gttgctcgtc
    11221 atttagttcg gcattatcaa tcccttcttg tacaaagtct cttcctaaaa tacgatctgc
    11281 ttcttgtaac aactttaatg tcggagctac ttgtttcaaa aaccagcgtt ctgaacgatc
    11341 taatgagaat ttttctggct ttactgtaaa atccaattct tccatcgaac gaaataattt
    11401 tgcccatcgt ttatcaacaa tccgtgcatc actttccagt ctatcccaaa caataagttt
    11461 tgcattaatt aaaccaacgc caatctctcc taaacgtttc ggtgaatcac ctaaaataaa
    11521 tgcttctact gcttgtgttg ctttctcatt ggctaaccgt aactcatatc gattataaat
    11581 accatatttc gttaatgctt ctaattctga aattttttct cggttcgcaa tttcaaaacg
    11641 tttctcataa aaattaaaaa agagaggact ctttcgcgat ccaaaataca aagataatcc
    11701 ttcacttcga aaatagccat catctaaaaa acaaccgcca atattctgga atctcttaaa
    11761 gtccatttct aataaacctg ctttttgttt ttctagtaat tcaaagagat cgaaatgttc
    11821 ttccgtttcc aatccccaaa gttcatctaa cgcaatatcg aatcggctac attgcacacg
    11881 tgtataacca gcttccaact ttccttgatt tttccaaatg gaatatagcc atgttcgcca
    11941 atctgtttgt tgttcacgca tgacttcctc atattcccgg caaccttgtc ctgaaaattg
    12001 cacaagtaca ttttctgtta cagttcgttt tgaatgccag aacacccaga tattcccacg
    12061 aacagaaaga cagtcaaaaa gatacaagcc cgttggacgt gtttcaaatt ctgtcagatc
    12121 cattcgtaat acacgttgaa tcactgtttt gtagtctgtc ccaaaaaagg aaagacgcaa
    12181 ataatcaatc attgctcgaa gatctattcc gcttttcata tacaatcgaa agcgttcctt
    12241 gattcgttgt tcaaaatcct ctgggatttt tcttttccct tgttccatct ttctaatgta
    12301 atcttttgag cagcccatca attgcgcaaa tcttgcttgt gatagcggct ttttatctcc
    12361 acttatatta tgaattgcac ttaacttctt tctgtagctt tttagctcaa tcggtgtcat
    12421 tttctcgctc cttttataaa ataacaaaat cgattttttt taacccttta gaaacaccga
    12481 tatatcaact agtccccctc gttttttggg aaaagtagta caactgctga ccttagataa
    12541 tcacttgata caaaagggtt tataaaactt ttttctatgt gttatgttat ttctgccccc
    12601 catattatat atcggggggc tttacacaaa gaggccgtgc gcaagcgcac gacctctttg
    12661 tgagctgcac acgctgctag cgagtgcgca ttataggccc atgcttcgca gccttaatgc
    12721 gcaccgccag gcagagtgcg gctaccagac tacgtctaga gccctcccga tagataataa
    12781 ctacaaaatt aaatgatcta attttgttat ttcactatat ttttcaggag aaaaaacgcg
    12841 ataaattgac aaagttaaat cctcttctaa atctactcga taatgataca atcgagtcaa
    12901 tcgttctata tcttttttcg ttagcaatac ttgattaata acttttcctg taaaaacacg
    12961 ttctttgcgc aaaaagcggt aatcagttac ttgttcaata cgattcaacc aatcataaaa
    13021 cgttctggaa gaaagagaag gattaatcat tcgtattgct gtagctactc tataataaca
    13081 actctctgcc atttaacttc acctccttag ttgattacaa aatatgcgca tcagaaaaac
    13141 ttgttccttt tggatctttt gctaaataca tttggcgctc tagttgttct tgtaacaact
    13201 gattatcatc tcccatcgtt tgatcttgac tatcctcttt tactgtaaaa tcatagacaa
    13261 tcatttcagt aaatttctca aattctttga taaagtgata atctttgtaa acaaatggcg
    13321 caaaaaactc tacaggtaca tcaaatcctg gaaaagcata tccacgacca ggttgatctt
    13381 tgttgtaaaa tggcttattt ttaaaatctt ctccaaaagt cattaaataa ccaatgggag
    13441 acaatcgacc aacagaaata cggaatgtta aattatcccg aacaccactt ggaaaatatt
    13501 ctgcatctgg tcgttgcatt gctaaaataa tgtacatccc tgcttgacga ccctttaata
    13561 ctaatggctg aatcaattta tcgactctat ttctcgcttc catatccaac gaaccataaa
    13621 aagctgccca ttcatcaaaa aagataaaat gcggtggtaa cccataataa gcataatttt
    13681 tccctggttc atatttagga tctttcatgg ttttcatata ggcataccgt tgattcattt
    13741 cttttaatgc atttcgtaaa caaataatca tttcttcatt tattttgtaa aacacatgtc
    13801 ctttgaatac atcgacgtca cctaatgctg ctaaatctgc cgcttttcca tcgcaaatat
    13861 ctactgtccc aacacttaaa caagcttgaa ttaaagaata caataaatag gttttcccac
    13921 caccgattgc accagcaatc aacatatttg gtgattgatc atatacccaa tccaaagttt
    13981 tcattaaatg aatacgtcca ttttctgcta tacattgatc aataggaatc cgaccagaat
    14041 ctgaaaggaa tacataattg attcgggctt tttctcgttc ttcacgaatt aaatcacgca
    14101 aaaatacttc agataacaaa tgacccattt ttaaaaatcg ttcgtgaaaa cgcccaccat
    14161 ctaaaggaaa agaaaccact aattcttgtt tcttttgata aaaataaact tttggtaatc
    14221 tcattttttc ccttgtttgt ttctcactaa cttgtttctt tgtataaaca aaccgattgt
    14281 caattaacat tcgaacaagt aactgacgcc aataaacaga ggcaaaatat ccttctttta
    14341 gtcgcgacca atgcatcaac cctaaaatta acccactaac cagcaacaaa tacaacacaa
    14401 tacttattcc aggaataaga tacatttgcc aattcgaaaa cgtacttaaa cgtggataaa
    14461 atactttcca actaaaccaa cctaacagaa tcaaaaatgg aaacaaaaag aaaccataat
    14521 agcaaaacca taaataacga ttgaaatagc gaattcgccg accacgatac ttgaaaaaat
    14581 gagctttcaa ttgtcacatt cctcctttct gtttttactt ttcctgtgct aaaaactttt
    14641 gacaaaaagc aactaataca ggaatatcac atttagtaaa aaaacataat cgaaaaagat
    14701 ttaatcgatt tttataaggg tgtgcacaca tattacatat tcgtactata cgaatccaaa
    14761 tcacttcctg atgatcttgt ttccactggt acggtaatga aaaacgaaca agatcacgta
    14821 aaaacataca ctgcccaata accgcacaaa tatatgcatt tgtttgtaca aaaatcgttg
    14881 gattgtctcc aaaatactca atcaattgtt ggatattttg ataacaagca caatctctca
    14941 aagtatttga ttggttttat ctgaattcaa aatctcccct tccttctatc ttagataaaa
    15001 agaataggaa gactccttcc tattctttca aaaaaacctt attttttctc tttttcctta
    15061 gaatcaacac ttggttgttt ttgatctaaa cctttttctt gtgattgttt catattagga
    15121 ttatttgttg gagcttctac tttctctaat cctttcgcat aaatcactaa atctgaatat
    15181 tcacgattgc catcttgtgc tggaatatat accacatgat attctggatc aattaatttc
    15241 acaggatcac gtcgtttaaa accagtcaaa tctacatctg ctggaaattt aactcgtcgt
    15301 gcaatctttg gttcatctgg atttccaaaa actaattgtg ctcgctgtga tcgaatttct
    15361 cccgtataat cgcctgtttc attatcaaat acacgatcag actcttctaa tgcgccaaag
    15421 cgtacggctc ctaaacaagt ttcatctaat gcttctgtaa tgtcataatt atttggaaat
    15481 tttaatccca tcttacaaaa cctccccaat ttttacacca gttaaaaaga caccaacatc
    15541 ttgatagcca gttgttcgac gctctgtttg tcccatatca tttgtttcac gactattcac
    15601 tcgttcttta cgaaaaacaa aataagcttc ttcaaatgaa atacgcacct ctttttctgg
    15661 taatcgtaat cttttatcct cttcagctgt gagataaaca aaaaaagcag gctgttcttc
    15721 tcgcgaacac tctgcttgta attgaacata tcgcccagac acaccattaa tttcctttgg
    15781 tggattaggt tcccgtgaac ccaataaaaa tacttgacct aaaaactctt tcttcatacg
    15841 caatgctttt tctgccataa agtttttcct cctcttaaaa accaaaataa attctaaata
    15901 tttctacatt acaatcaaac aagcggttaa aaatcctaac cgcttgttcc tcatgactaa
    15961 tctttggctt tacgtacttt ataaagtgtt atacctaaag caatgactaa tactactaat
    16021 ccaagaacca taagtatact acttgtttgt tcacctgttt ttggcaacat tggtacaaca
    16081 gtagctggtt taacccgttg ttctggtttc actggttgcg gtggttttgg cgcttccaca
    16141 atttgaaccg tttgatcttt gtttgttaga tctacattgt gtttcgccac ttcttcaaac
    16201 gtttctttct catcttccgt tttcgcttgg agccattccg caaaactcaa gccttttcct
    16261 gcaagattgc gaccgtccac taagaattca aataagaatt ccatcatttc tttatcggcc
    16321 gcaaagactt tttccgcatg agtgccttca aatggtgtgc cgtctggtgt taagtagcct
    16381 tgaacaatta agcgatagat ttgaccaatc gcaagaccgt ctactttgac ccattcaagc
    16441 acagtttgat ttggcaaggc caagatctct tttccgccat ttttattcgt cgctttcgtc
    16501 gtaatggttg gattactgat tttgatggtt tggtcattgt cggtaatatc tgcgtgagaa
    16561 gccaataata cgccttctct aaacagatct tcaaagacca cgacttcttt gcctcgcaac
    16621 gcacgtgcgt ctagaataaa gtctagattg actgtaccat ccgttgtttc tgccacaaac
    16681 gttaaggtac ttgtgacttc ttttccatct actagcactg gtttttccgt tactttgtcc
    16741 attagaacac cgttgactgt gtatgttttt ccaacgacca aatcgtggta tttcacagcg
    16801 tctgtaatgg tgactttcgt taatggatta acctctttca agccagtggg tttatgtgta
    16861 gccgttgtac gtacacttgg gttagttact cgtaccgttt gccctttgtc gttgatgtct
    16921 ttatggtttg ctagttcttc atcatttcta aaaagacttt caaacacgac gatttcacga
    16981 ccatttatac ctgtcaaatc aaaggtaaat ggtacgatca ccgtttcatt ttctgctttg
    17041 gcaataaagc caacactcgt ttctaccgta tttccatgca caagcaaggg ttcaccggtt
    17101 gctttatcca ttaagatccc tttgaccaag tatgcttttc ctggaagaat ctcggtaatt
    17161 tcaacggtat cgtttaaggt aaacaatcct gttgggtctg cgactgtcaa tccgtcttca
    17221 aagacagccg tggtatgaac ggttggatta ttcactttca cactttgggc ttcatcgttg
    17281 atatccgcat gtgctgcaac ttcaatgcca tcacggaaaa gtttttcaaa aacaacgatc
    17341 gtttgacctt tcagaacact agcattaacc acaaattcaa ggtcttttgt gccattgact
    17401 ttgtctgcaa taaaggccaa ggtacttgtg atttctttgc catcttgtaa aattggcgca
    17461 ttcgttgcct tgttcatcaa tactcctgaa atggtgtatt cttttccgac aaacaagttg
    17521 gtataagaaa cactgtcttt aatgacgatc gtttctaatg gatcaaccac tttttcacca
    17581 tcggctttat ttgacgcttg tgtttctagt tttggtggaa tcaagtaatt acgtacagaa
    17641 accactaacg tttccccatc atctggcgca ttttgtttga atgcaaccgt gaatgcttgt
    17701 ttatctgtat ttggttggaa actttctaat ggggcatttt ccaagaagta atagtcatcg
    17761 gcaactagac ctttcactaa aatttccccg ttttgatcag tggtatattc accaattgct
    17821 tgatttttac cgttatacaa ggtaaagttc acacctgcga gcggcgtttt cttcttgttc
    17881 cattcttcat acttgaataa ttttacactt ttccgtgctt gacggttcgc aaaatggatc
    17941 actttgatcg gttctgtatc attggtaatc gagaacacta atgggttttg attcaaaacg
    18001 taatttacgg gggctttgat ttcttccaaa cgatagcgat cttttccata agtaagtgta
    18061 ccgttagtta ggaaaaagcc tttttcattg gttttaaaga tatccgttgt ttctgtatca
    18121 tttggaatct ccattgtgac ataacgattg gctaaagtat caaaaatctt gaattctgta
    18181 ttagcaagtg gaatcacttt tcctgtttct tgatccactt tcaccacttt aagacgttgt
    18241 tcaattactt tatcttctaa aacataatgg aaagtttgcc cattttcaga aattgtgact
    18301 tcaagtggtt caaatagttt atacccttct tttcctttgg tttcagaaag aatgtacgta
    18361 tcaaagacaa cggtattttt accaaaagta gcataaccat cattgtctgt cacaacggtt
    18421 ttcaccactt tgccagttgt ttttgacgtc aaagtgattt caatgccagc taattttggt
    18481 ttgttatctg gattcgttga atcgactaaa tctttattag caactttcac taaatcaatg
    18541 gttcctttga tcacttgatc ttttgcggtc gcattgacag tttttccagg ttcaacaaca
    18601 atttcaattg cttcatcaga aagcacataa ccttttggcg ctttgatttc ttttaaaaca
    18661 tatttgccag gatcaagatc tttaatttct gcctgtactt tgccgttaat ttttttcaag
    18721 gtaatttctt taatcttttt cccatctgct tgatacaaac catagaccgc attttctaaa
    18781 gaagctgcac cttgtgcttt cacaccagtt tccgcatctt ctttaaacac acgaatgttt
    18841 ccttgtttta gaatttgtaa attaataaca gacgcattag gttctgttgg atgtaacaca
    18901 ccaattgttt gagctcctgg acgacgatag gctaatggcg caccttgaac tttaatttta
    18961 ttcaattgaa tttttgcttt atccggtgcc tcttttgtga ctgtaatcca tacttcattg
    19021 ccgtgaatag atacattgac accttttggt ttttgaatgt ctttcacata tttcaaaaaa
    19081 tcatttttat tctttacttt caacgtttcg ccaattttta ccgtatgtgt ttgattatta
    19141 aaagttgctt tttcaccaaa gttattaatc aaacgttgaa tttctgcttt tcgacgatca
    19201 taatcaacac ggatattttt gattgggatt tttgtgccca aagcttccca aatcataaat
    19261 tgtgccgcaa tgtattgttc atcggatttg tctttatttg tgacatatcc taaataagag
    19321 atcaagcttg tttttttccg tacatttgaa tttttcaaat agtttttcaa agcagtagct
    19381 gtataaactt gaccttctgc ggctgagaca ccaggctcaa tacaaaacgc cacttgtcca
    19441 ttaatctcaa aacgaaccat ataatttaaa gtgaagttta gattgttcgg actagaagta
    19501 acaccaatat tttcgcccca atcaattttc cgaccaccta aaacagctgc attggttgca
    19561 cggcgaacac gaaccgaacg attggttggt actgttacta acagtgtagg tgtacctgtt
    19621 tttggaatgt tctgctcttt tttcttttga ttttcaatac tagccgtaga ttctttgttt
    19681 ggtttcgttg gttgattttg actagctgcg gttgaagaga ttgttttttc aacgttttcc
    19741 tcattttttc cttctttgtc agcagtagcc gctggttgtt ccactgtttc ttctattcgt
    19801 ttcttttctt ccgcttgttt ctcagaagac tctggctgca cagaaggcgt tgcttcggca
    19861 gctttttctc cttctagagt ggacgtttct gaagcagggg ttgtaattga ggcagtttct
    19921 tcaactggtt gtgaagtacc aggactattc cctgggtgtt gtgtttcccc aatcgtctga
    19981 tcgagagtcg ttttggaatt gaccgttggc gcttctgtca caggggcctg ttcaacagtg
    20041 gcttcaccag ctgggacccc ggcttctgct ggttggactt ctgtcacttc agaagcgact
    20101 tgggcaatgg cttccatcgt aggttgtata gccgtcgttt cttctgcatg tgcttgagca
    20161 gttcccacca agaaacagaa ttgagaaaaa gccaccaatg cgagtgcttt caaccaattc
    20221 attttcgttt ttgcatacac aagaattcct ccttttcctg agaattttat ataataaaac
    20281 aaaacgccgt taccaaaagt gataacgacg tgaactgtct cttctatttt tatctggcgc
    20341 tcttatctga acacatttca tcaatgattt taccctccca ctataattac agaacaaaaa
    20401 aagaacctgc ttcgtggaaa tcaactgtcc atttcccaat tggaatacca gcttttttgg
    20461 caacttcacc atcaccaaat ctcatgccat accaattact accttcatcc ttaatttctt
    20521 tccaaccata tgacatagct tcatctaatg aatcaaaaat catgacacta ttgccaattg
    20581 ttacaggatc tggcttaaga tcttctggaa gagaaatatc aatcatacca taatgatccg
    20641 attcctcagt attattgtca ttactagcta ctggtttggt tgttctactg gcttattttg
    20701 atacaacaag tactcttatt cttcttttac ttctccgttt tcaatttccc attcaaacca
    20761 ctccgcaaag tcttgcttaa aaccatctac tcgataccta ttttgtaaat aggcatcttc
    20821 tgacgtcgta ggatctaaat aggtaggttt attctctgga cgagcataga cacctaccca
    20881 tgtttcgtaa tctccttcaa aataactatc taccaaatag cctttttttc taagaatctc
    20941 ccgaacttga tttttaatta ttgatacttg ttcaaaacca tcactcattc tatcacctca
    21001 aataactcta gattctcata aatattcccg acaatttcaa tcctatcaat gatctctaac
    21061 aaatcgctac ttatgcttcc ccattcgtat aagaatttcc cttccgcaaa ataaattttc
    21121 cataaacttc ttcatgatcg tcccaaccaa tatcactctc gaaaaattcc acgttgttct
    21181 tatcttttag ctcagtaaat gccataagtt caacatgttt tccaacctcg aaacaatcac
    21241 accacccatc aaccgttaca cgtgtgcctt caatacctaa gaaactttgt cctcgtgtat
    21301 taaacactaa aactttccgc attttttgct cattaattaa tcacgcgcga aatctaggaa
    21361 tcacaactta ttcgcttctt ctttataaat aaaaaaactc caataatcgg aaattactat
    21421 cttttcctca ttcctttttt aaatcttcat acttcttctt atctttgtcc taacctaata
    21481 acacaaattt tgaagaaaaa ctcagtaaaa cctgattctt tttttatctt cacaacaacc
    21541 aactttttct tcagatcctt ttactagaac ttgtatacaa aaattgttga atgctgatat
    21601 aatttgcggc tacttgaaaa aacgatagat gtaaaaagat aaatagaatc attttttatt
    21661 ctatatcagt ctatgattca ttgcttatca atcacccaac ccattcgatg ccgttttttt
    21721 tagagaaatt taaaaaagac cgtacgaatc tcgcattatc cgattacttt tccagaaaag
    21781 atccccaatt ctgtataaat aaccctatct tcttgttggt ttcattttct catataagaa
    21841 ttcagtcaat ctacccacta tgaccattac tacgtccaac acaactgaca tattaaccac
    21901 gtaagtattc cctcacagat ctccgaatct ccatggtatg gattcccatc ctgacgctgc
    21961 agaggacaac atatttcatg tggctctgta cccttttgat ttggaaacac tacaaatcaa
    22021 tactcatcta taacttatca agcgaacttt cgctaggctt ctagtgatta ctaaattgtt
    22081 ggataacata catagcccat tttatctagc tgttattcat aacagttagt tcttgtatta
    22141 cttcttccgc ttgtgttgcc attgagcttg tcaaaaagtt acttagaaaa aagctgccag
    22201 taaagcaact ttcaacaaac cctttttgtt tcttatcata tcaagaaacc tcctttta
//
LOCUS       DANKHL010000027        21992 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000027 DANKHL010000000
VERSION     DANKHL010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.029"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            201..1166
                     /locus_tag="PW101_000712"
     CDS             201..1166
                     /locus_tag="PW101_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL2513137.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(1265..2437)
                     /locus_tag="PW101_000713"
     CDS             complement(1265..2437)
                     /locus_tag="PW101_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2513138.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(2424..3569)
                     /locus_tag="PW101_000714"
     CDS             complement(2424..3569)
                     /locus_tag="PW101_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2513139.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(3583..4507)
                     /locus_tag="PW101_000715"
                     /pseudo
     CDS             complement(3583..4507)
                     /locus_tag="PW101_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            4624..5703
                     /locus_tag="PW101_000716"
     CDS             4624..5703
                     /locus_tag="PW101_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2513140.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            5735..6328
                     /locus_tag="PW101_000717"
     CDS             5735..6328
                     /locus_tag="PW101_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2513141.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(6438..6665)
                     /locus_tag="PW101_000718"
     CDS             complement(6438..6665)
                     /locus_tag="PW101_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2513142.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(6768..9356)
                     /locus_tag="PW101_000719"
     CDS             complement(6768..9356)
                     /locus_tag="PW101_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDL2513143.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(9376..10035)
                     /locus_tag="PW101_000720"
     CDS             complement(9376..10035)
                     /locus_tag="PW101_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2513144.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(10084..10587)
                     /gene="trmL"
                     /locus_tag="PW101_000721"
     CDS             complement(10084..10587)
                     /gene="trmL"
                     /locus_tag="PW101_000721"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDL2513145.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(10808..11674)
                     /locus_tag="PW101_000722"
     CDS             complement(10808..11674)
                     /locus_tag="PW101_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2513146.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            11783..12424
                     /locus_tag="PW101_000723"
     CDS             11783..12424
                     /locus_tag="PW101_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDL2513147.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(12486..13847)
                     /gene="mgtE"
                     /locus_tag="PW101_000724"
     CDS             complement(12486..13847)
                     /gene="mgtE"
                     /locus_tag="PW101_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDL2513148.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(13865..14764)
                     /locus_tag="PW101_000725"
     CDS             complement(13865..14764)
                     /locus_tag="PW101_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2513149.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(14766..15563)
                     /locus_tag="PW101_000726"
     CDS             complement(14766..15563)
                     /locus_tag="PW101_000726"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDL2513150.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(15541..16245)
                     /locus_tag="PW101_000727"
     CDS             complement(15541..16245)
                     /locus_tag="PW101_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDL2513151.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            16376..16951
                     /locus_tag="PW101_000728"
     CDS             16376..16951
                     /locus_tag="PW101_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDL2513152.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            17069..17746
                     /locus_tag="PW101_000729"
     CDS             17069..17746
                     /locus_tag="PW101_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL2513153.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(17825..19633)
                     /gene="pepF"
                     /locus_tag="PW101_000730"
     CDS             complement(17825..19633)
                     /gene="pepF"
                     /locus_tag="PW101_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDL2513154.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(19809..20807)
                     /locus_tag="PW101_000731"
     CDS             complement(19809..20807)
                     /locus_tag="PW101_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDL2513155.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(21033..21686)
                     /locus_tag="PW101_000732"
     CDS             complement(21033..21686)
                     /locus_tag="PW101_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDL2513156.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         6492 a         4698 c         3814 g         6988 t
ORIGIN      
        1 ttttttgtgt ttttcggtct tcggaccttt ttcattttcc tagacttctg ctataataac
       61 caagaattaa ttttgtaaca gatttttttg aaaaaacaag tttctttcat tcaaaaaata
      121 gaaaaagctt gctaattatt ggttaaccag caaacaggag tattaaaata tcttgtttta
      181 ttgaatagca ggaggaaatc atgaaattcc attatcatat attgatcaat ccatcggcag
      241 gcagcggaaa tggctataaa gtagcagaaa gaattttacc ggtattaaaa aataagcata
      301 ttgactatac acttcattat agtgaataca aaggacacga tgctgagatt gctgaaactt
      361 tggcaaaaga aacattgatt ccttggatag aagaagaaca gaaaacaatc gatactttcc
      421 cccttttgat catcgtagga ggtgacggca ccttacatca ggtacttgat actttctatc
      481 agatggaagt agaattccca gttgcttata ttccagcagg ttctggagat gattttgctc
      541 gaggggcagg ccttccaaaa aatcccaaaa aaggacttca gctgatttta gcagctcaat
      601 caccagaaaa agtccacata atggcttatg aagaaaaaat aagtgaaaaa aaaggaatag
      661 ctgttaataa tttcggtata ggattagatg cagctatcgt ccatgcgaca aatcattctt
      721 ccacaaaaaa gcgattgaac aaatacaact taggatcatt ttcgtattta ttctctattc
      781 tccgtgcttt attcactcaa aaaggatttc caattctggt agatgcagga ggaaaacaat
      841 taagtttttc aaacgcattt ttatgtacag caacgaagca tccttttttt ggcggaggaa
      901 tcgcgattgt tccaactgct gatgcgtcta aaccagtcat tgattttgtc gtggtcgaac
      961 gaatcaattt ctttaaaatc ttgtggctca ttcttttact tatacagaaa aaacaattga
     1021 aatcgaaata tttccatcac tacacaacaa acagattacg tatcgtttca acaacaccgc
     1081 aacatggtca agaagatgga gaagaaatgg aaaaacgacc ttttgatatc acaataacca
     1141 caactaaaca gttgttttgg tgttaataaa agaggttttg tcagaagcta catctggaac
     1201 accgtttatt cggaaaaaag gactgtgaca cgacttttgt cacagtcctt tttttactat
     1261 gcagttaatt ctttgcccaa ttgggttgcc atcgatagtt tctttcttta cccaaaataa
     1321 attggatcat tgaaaacacc gcagcaaaag caactaagac acaaccattg aaccaaaaag
     1381 attctgcaaa tgtttgattg taactgagtg tttcactgat caagttatta ttttgaacaa
     1441 accaatatgt tggtgtgaat gccgctattt tgctaaccat ttctggcaag tattctgctg
     1501 gtacaaacac cccaccgata aagcaactgc ctgtgataaa aatatctttg atcccaccgc
     1561 tggtttctga gttttttacc aatgaagtga tacaagccga aaagctaatg atcggcaata
     1621 aaaacaagaa agtatttaaa atatgatacc ataccgttat atcccacgag gtgtggatca
     1681 ctaacagaac cattagaagt aagaagccac tgaacaacag cagcgagtaa ctaatcaaac
     1741 taaaggttac tttgcgattc aagtttcgtt tggtaatggg cgcactctgg tttcgtttac
     1801 tgatctcttt gcgattaaat gctagattga tgacgctgaa accgctaaaa atggatagaa
     1861 acaacccata agacaagaaa ttaaaggttg tgcccgtcaa cagctttttc atccgctgac
     1921 tatatcccgc acttagctca acttctccag taaccgagag tgccatttgt gtctgttcca
     1981 tagctgcatc gattgtttta tcttcttctt gaaacagaat cagtgtttgc aaaaaggtgt
     2041 tcacttgctg tgtaacaact gttttagaaa aggcatctgg gcgactctgt atcttgatcg
     2101 tcggcatctt tcctacttcg acttgttcgg taaagtctgc aggcagatgt aagataatgt
     2161 ttacacgatt aaaataaaga gcatcatcga tttctttttg tgaggttcca tccaatgtaa
     2221 cgactgtttc cttgtcattc aaatatttac gaaggccatc gactacaggc gactcttctt
     2281 cactaatcaa ggcgattttc gcatcttgca gagaggtatt ttcaacatca atattttgcg
     2341 caaaaaagaa tgtggcaaca acagtgataa ttacacctat cagtaaattc gatttatttg
     2401 ctttaagcaa tgtccaaatc gttttataaa gcgtcataac gcacccctct ttccatccaa
     2461 gcatttccta acgcaaaaag gaaaataaat ccgatcaacc atagaagatt ggtataaaaa
     2521 ctagccaaat cagtgtagaa ataaagttta tacaaacttt cgctgatcaa gttgacgaga
     2581 tttaattgac caacaatcgg aatatgaaga tcaatccaat acttcacttg ttccgacccc
     2641 atcataccag caaaaaagct catcgcaatc gtcactgaaa tcaagatgct atccttttgc
     2701 accagattca tttttggtat caaattacca accaatgtac ctaacaaaat cgcacacaac
     2761 gaccccagag cggtaacaat catcaaccat tgccaacgtt caccaaagtc tacttgatag
     2821 ataaaacgaa agacagctaa cataatataa atctctgtca aaacaatcgt aaagcttgct
     2881 gccaaattag cgattgaaac taacgattgc tttctaggaa tcaaggacaa gcgaatacca
     2941 tttgacgatt gatttgcttg ctgatcctgt acattgcgga ccccccacat cgtcccgtag
     3001 agaatcgcca ttccaatcag agtgaagaaa taaaaacttt tgaaactcat attacgacct
     3061 gcatgaactt cttgaaaaga tacttcttgt tcaaaaacag tcatgatggg cgcgagatca
     3121 acgttctcca cttctgccac tttactcgaa attataccaa tacgctgtaa aaattgatcc
     3181 atgacgtttt tcatcaacgt ctgactcatt cctgcttgac ccacatggag cgaaacagat
     3241 gaaccatcaa tgagataaaa accagccaaa tcatctgcat ttagcttttc attcgcttct
     3301 acttccgata gttcctgtac ttcaaataat ggtttatcat tgacttcgac ttgttctaag
     3361 actgtggtaa aattttccga aagcgctgtt tgatccgctt tgattacaat tccagtatta
     3421 atcgtttcga gattgccacc ttgatcaatc tcgccaaagg ccgccataaa catcaatcct
     3481 aaaagaattg gaaaggccaa tgtccaaaac aacatggatt tatttcgcag cagtattttt
     3541 gtgcgatatt tataaaaatg ccaaaacatt atttcccctc ctctagtctc tcaaatcctt
     3601 gccagtaatc tctaaaaaga catcattcaa ggacgcttct ttggttgcta tttttccgta
     3661 actgatcttt tcttcttgta agattgtaag aatcggcaac aatactgtat ttagttcagt
     3721 agttgttact tggagctgac cttttttata agcgattcca tcagaagcta ttgttcgctg
     3781 acaacacgtt ttctgggagt aatgggatgt cgatcgtcaa acgatccgcc gtccgtacca
     3841 tatcttttag agattctttg gttcctttag caatcacctg tccttgatcc atgatcacga
     3901 tctggtcgca aagaatttct acttcttcca tgtaatgtgt tgtatagaca atggttgctc
     3961 cttgtcgatt cagctccttg atactttcta aaattttatt ccgactttgg gggtctacag
     4021 ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg ggcaatccca catgcgatgt
     4081 tcaaccgacg tttcaagcca ccactaagtt ctcctggacg aaacgaaaca tatttttcta
     4141 atcccactaa ttgaatcacc tcttcaatca aggctttcct tttctctttt tcggaaacat
     4201 ataaaccgca aaaataatcg atattttctt gcacacttag ttcgtcaaag accgcaacgt
     4261 cttgaggaac gaccccaatc attgccttga cagcataatt ttcaggagtc attgttgtcc
     4321 cgaacaattt aatggttcct ttgtcataat ttaacaaaga aagcatacag ttgatggcag
     4381 tcgatttccc actgccgttt ggtcctaaca gacctaagac ttctccttct tgtacttgca
     4441 gatcaaaatg attcaatgct acaaaatctc catagcgttt gactaattta tcaatttcga
     4501 taatcatcat gaaaaactcc ttccaaattt cttgttgatc tgactataat aaagtgcaag
     4561 aaaagaaact agtgacgatt ggctaaacca cacatgacat ttgtcacaat gaaggagtct
     4621 gctatgcata aatctcggct actcaacgaa actagctttc tactggttat ctcattgctt
     4681 ttgctggtaa gtcaaggatt cgttgctatg caagttatct atttattgat cgccgtttta
     4741 ttcactggtg tcagttattt actgcctaca aagccaaatt ggattttcca agttattcta
     4801 tttcttttat gtgccttttt acctgatctt gtcctgtttc tgccagcgac gatgcgagtg
     4861 atcgcccctg aagaaagcca ccttgattac ttgtttttgt taggtttttt gttgatttcg
     4921 acggttggta atcatccccc catcaaggtc gcctttgtcg taattttgct gtttttttcc
     4981 ttttacttac gaaaaaaaga tcaacgaacg accgatcttg agaaaaagta cattgtctcc
     5041 aaagatactc attgggaaaa acaacaagag ttatcggaac aaaataaagc attggccctg
     5101 tcacaagaaa cacaattagc cttgcaaatt gtggaagaac ggaaccgaat tgcccgagac
     5161 atccatgaca atgtcggtca tctgctgtca agtgcgattt tacaaattgg tgcgcttgaa
     5221 atgatcaatc aagaaccaaa actaaaagaa ccattagaaa atttaagtgc caccgttcac
     5281 actgggatgg atcggattcg tcaaagtgtc catgacctcc atgagacatc gatctctttt
     5341 caacactcct tagagtttct gatcgctgat tttccttctt ctgttacgat tgaaggaaac
     5401 ttgtttgaag gattgaatga aacgcagcaa aactgcttct taatggtcat caaagaagcc
     5461 ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg ttttacattt tcgaacgttg
     5521 ccggcctttt atcgcttgca aatcacgaat gatggcacca gcccacaaaa aaattctgct
     5581 cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag aacaaaaggg acaagtccat
     5641 attttccaag aaaaccagca atttcagtta acgatcattt taccaaggga gggacaccaa
     5701 tgataacagt agtcattatt gagatgatcc atttgtgacg aaatcgttac aaaccatttt
     5761 agaaagcacg aaagagatcc gtgtgttagg cattggtcat tgtgccaagg atgcgctagt
     5821 cttatacgaa acccatcgtc ccgatgtttt gctgacggac atccgcatgc ctaaacaaac
     5881 gggcatcgat gctgccaaag aaattttggc taaatttccc aaagccatcc ttttgctttt
     5941 gacgacattc aaagatgaag aatacatccg cgaagcattt tccatcggtg tcaaagggta
     6001 tttaatcaaa cagaacttgc aagcgattat tccttctgtc aaatccgctt ataatggaca
     6061 ggccgtattt ggtaatgaaa tcatcgagac tttcactcaa atgatgaaga ataaaccgac
     6121 gattgatagt catgcttatg cttcttttag tgaacgtgag cttgcaatca tcaaagaagt
     6181 agctgccggg aaaaacaata aagaaattgc agatgccttg tacttgagtg acggcactgt
     6241 tcgcaattac atcagccaat tactagaaaa gctagattta cgggatcgca cgcaattggc
     6301 tatctatttt tatcagcatt tgcaataagt cttatctaac acaaaaaaac ctgaatgatg
     6361 ctccttcgca tcattcaggt ttttttcaca tcttctattc aattgtcccg caaaccattt
     6421 cctcacgatt gaaagaatca aggattcgca gggctgccag gattcgttgg attgttcggc
     6481 gtgttaggcg gaaaaatagg cgccttgtct aacgtttttt ctagttttga ttcttcacga
     6541 tcgtctcgtt ctacatcagg actttcattc aatttgaatt ctttttccat ctcatctaac
     6601 tttttaaagt cattgccaga ttcttttgct aatttcgcta atttatcttc ggtttcttta
     6661 gccataaagt aacgcccccc ttttgatttc agcttagcat atcacactga aaaaacaaaa
     6721 gaaaacgcta accaacgatc atttttagct atttctcatc tttaaaatca agcatttttt
     6781 gccggtataa aatcgtaggg agaactgttt tccataccaa tcatcggatc tactaactgg
     6841 ttatcgatca cttctgctgt aagaacgaaa tctttagaaa cagcaggttc tacctcttct
     6901 ttcttttctt ctccaacaaa tgaagaaaac agatctggtt ccactgtttc cattacttgg
     6961 tttttcgttt cattcgtccc actgtcatct tctacaggta gcttttcttt tacaacccgt
     7021 ggctcttttg tatagttccc atcttcaaat ggatcatttc cgaccaaaga atcttcataa
     7081 gctgctagtt tggtccggag agtgatattc attttatcat tatccatgat ccttgatttc
     7141 aacgtagtca tccggcttgc tgattcattc gctacacaag tgacataggc ttgctcttcc
     7201 cctaacaaaa tcaacagttc cttactgcga gtcactgccg tgtagagcaa attacgctga
     7261 agcatccgct gatattgatg gaccatgggc aaaataacca tcttgaattc actgccttgt
     7321 gatttatgga ttgagcaaca ataagaaaga gtgattttat tccattcgtt tcttttataa
     7381 gtgacctcat ttgcatcaaa ctggataact aattcatcca ctttatcttc agaatctttt
     7441 gccaagatga tacccgtgat ttctcccata tctccattga atacattcaa ttcgggagta
     7501 ttgactaatt ggaggacttt gtctcctatc cgataaacgg tgtcgttcca atgtacttcc
     7561 ttttttccag gtttccctgg attgaatatc tcctgcatca ttttatttaa cgcatcgatc
     7621 cctgcaggtc cccgatacat tggtgctaat acttggatat cttgtgcacg gaatccctta
     7681 tttttcgctt tttccacaat tttagagatc aagggttcaa tttgataagc atcacttcgg
     7741 aaataggaac gatctttttg attctttgtg aaatcttttg gcagacgtcc ttctttgatc
     7801 tcatgggcaa gaggaataat actagaacca tcaccttgcc gataaatctg attgagttcc
     7861 attttgggaa tttgttcgat ttccaaaaga tcatggagta cttgaccagg accgacagag
     7921 ggaagctgat ctttgtctcc tactaagata acttgcatat tcgtcggaat cgctttgaat
     7981 aatgtgttag ccagccatgt atcgaccata gacatctcat caacaatcaa aagtccgcct
     8041 tctaattctt tcgtactgac tgaaggtgtt ttttcccgtc cattcagccc cagaagacga
     8101 tggatcgtac tggcaggcag acctgtcgtt tcgttcattc gtttagctgc tcggccagtt
     8161 ggtgctgcaa gtagaatagg aaaaatttct tgggtatatt ttgaaggatc caattccagt
     8221 ccgttcaact ctgcaaaaag attgacgatt ccgttgatca cagtagtttt ccccgtacct
     8281 ggtcctcctg ttaaaataaa tagaggggaa cggattgctt cagcgatagc ttgttcttgc
     8341 gaaggtccat actgtatgcc gaatattttt tcgatacgcc gaatattctt ctgcacttct
     8401 ttttcaggat aatggatttc ttttttacga ctgagaaggc gctggatcga attaccgatc
     8461 ccccattcag aaaagtacaa actgttttcg taaagattag tctcttcttg ttgtatcttt
     8521 ccttcttcaa caagttggat aattacagta gcaacctgat caggctggat ttcaattggg
     8581 cgacttgttt ctaaaatacg gatcgtttgc tgaagcaatg tctccgccgg tacgtatgta
     8641 tctcctgttt gaactgcatg ctgaaaaatc tgatgcaaga tagctgctcg aattcttctc
     8701 ggtgaatctg ctgcaatacc gatttgttcg gctagattat ctgcacgttt gaacccgatt
     8761 ccttctatgt cttcgactag ctggtacgga ttttcttcga tgatatccaa agactcgttc
     8821 ttataggcct ggtagatact gaaagctaac tgactaccga acccgtaacg attcagtccc
     8881 atgatcactt gatccatccc gtggtttagc cgaatcgtct cagcaagcat ttctcttttt
     8941 ggcttcgtca aaccggagat ttgttttaat accgaaggat cagcaatgat tttttcgata
     9001 gcatcttctc ccagcaattc tacaattttt tctgctgttt tcttcccgat tcctgggaat
     9061 ttttcgctgg aaagatacgc tacgatccca tttgcagttg ttggcttttc ttgctcatat
     9121 ctttcgagtt taagctgcct tccgtattta ggatgatcca ctaattctcc ataaaaacga
     9181 taagtctcat tttcttgaat atcgccgaaa cttccggtga ctacaatctc tttttcacgg
     9241 taatcagctg tggtttcact gatgtgtacc aaaagcactt tgtaaaaatt actaggattt
     9301 tgaaaaaaaa tcgcggcaac tgtaccaacg aaataaggca tttcttctgg ctgcatcgga
     9361 agccgcctcc ttcttctata gtaaaggttc tggtgctgtt tcagttccta aaaaatctgt
     9421 ttggttccat actttcagtt catatggcaa taatctgcaa ggctcttcag tctctaaaat
     9481 cgtcaaacta ctattgaaca atccacccat ttcacgcaac tcacttagct cttttcctgt
     9541 catccattga atggctgcag ttaaagaagc tccatgtcct acgaataaaa gtgggccatc
     9601 tttatgttgg gcaaccgcat ctgctacaac agtttcgata cgtgtgattg ccgattcaat
     9661 cgtttctccg tgaaaaggag aaggatcata cagatctaaa tgattacgca aattcggcag
     9721 attatccgga tatttctggt acatttcatc gatcgactga ccttctaaag tacccagccc
     9781 tagttctctt agcttatctg tataaaaaat ctctaccgga cgagcaagct ctttgttgat
     9841 accttctgct gtatcacgtg ctcgctttgc tgtactggaa taaattgctt taaaaggaac
     9901 atattttacc tttttcccaa atgctttgat ttcttcgtaa ctttgtggca aaagcggaga
     9961 atcgccattg ctcccttgaa aacgtctttc ctgattccat tgtgtcttcc catgtcgggt
    10021 aaaatatagt tccatacgca gcaaactcct gtctttcttt tattttactc tataataaac
    10081 tgtctaatcc agcttgtcat tttcatagtg atgcgtcaat tctaaattag ggaatccttg
    10141 ctggcgaagt gcttcataca ctatcattgc agctgtattc gataaattca atgagcggac
    10201 atgctcatca ttcataggga tgcgtaaaca cttttcttcg ttttcacgca taaatgtttc
    10261 tggcaaaccg gttgtttctt ttccaaataa aaagaagtgc tcttctccat cagcaaaatt
    10321 ttcgtcacta tatactttat gggcaaattt tgagatcaaa tgtagtttac gattacctaa
    10381 aaatgttaaa aaagcttcta aattactgtg gtaagtaata tttacatcat tccaataatc
    10441 caaccctgcc cgttttagat gtttgtcatc agttgaaaaa cctagtggct cgattaaatg
    10501 aagcggagta ttcgtggctg cacacgtacg ggcaatattt ccagtgtttg cagggattaa
    10561 tggttcaaat aaaacgatat gattcatcag gatcattcct ctcatattta tgttcgtttc
    10621 tattttctga caatttgatt tttgcataaa aaaaaacgta tcaactcggt gtcagtcact
    10681 gcgagtcgtt acgttagtga agctcttctc actaactttt atcagaacag gaacctgata
    10741 aaaaccaatg aaaaaacaac ttgttgctaa tataacaccc tcaacttttt tttgcaacct
    10801 ctactcatta aaaactgcta gaaaaacgtt ttctcttagt tccttttaat aattctatgt
    10861 caattgtaat cttcttcaaa ggattcttat ataagtctgc tttgattcgc tcttctacct
    10921 gccattctaa aggtgacatg gccaaaagat ctttttgatt ttcctctggt atctcaaatt
    10981 cataggttac acgctgacgt tcagttaatt tcattgcttc agcaaacttg gctactacac
    11041 gttcattgga ataactctgt ttttcaggtt gattcggata aaaagcctga cgtagttcct
    11101 ttaaataatt ttcttgtggc accaccttca acacttctcc atcttctgag agtacacgtt
    11161 ggaattcttg gtaatgagaa ggcgaaaaga tatttaaaac cgcgttgaat gtttgatcag
    11221 caaacggcaa attggtcaaa tcagctacac accaaaatgc ttcggcagat tgttctgtag
    11281 ctgcgtagac tccttctttt gaaatatcaa atccaacagc attttgaatc tttttcaatt
    11341 ttgaaagttc attcaagaag ctaccttctc cgcagccaac atctaatagc gagacttctt
    11401 caggtaaatg ctgcgcaata gtctcaataa cgggctgata catgccagat tggatcattc
    11461 tacgccttgg aacgaacatt ccttgatcgt attctgtttt aaccgcatgg gataaaaaat
    11521 aaagtgttcc ttttttggaa aggtcaaaac ggtgattatt tttgcatatc aagccattat
    11581 cagacttttt tacttcttct tggcaaaccg gacagcggaa cagctgatga ttttctgcca
    11641 aaaactggcg tcctcggtca atttttttta acatagttcc ccctcatttc tgttttatct
    11701 taccgttaga tgctcacttt ggcaactgct aaaaatatga tacaatgcct agaagaaagt
    11761 gaaagataaa ggagaagatt agatgatcaa agaattttgc gctgaaaact acacatctat
    11821 tcctctagca attgctagag gagccgatcg gatcgaattg tgtgacaact tagcggtcgg
    11881 tggcacaacc ccgagtactg gtgtgatcga agaaacgtta agttatgctg gggaaaaaga
    11941 tatccccgta atgacgatta ttcgtcctag agggggcgac ttcgtctata acgatatcga
    12001 attgaaaatc atgcatactg atttgattga agcaaagaaa ttagggacgg atggcgtagt
    12061 atttggttgt cttactcctt caggctggct agacgaagag gccttagaac tattgatcga
    12121 aacagcagaa gggttgcaga tcaccttcca tatggctttt gacagtattc ctgaagatcg
    12181 gcaattcgaa gcaatcgatt ggctggtaga acatggtgtt gaccgtatct tgactcatgg
    12241 cggtcctgct gacaaaacta ttgtagaaaa tttcccacgg cttaaagaat tgattgccta
    12301 tacagatggc cgcttaatca ttttgcctgg tggtggtgtc actgcagaga atgcagcaca
    12361 tgtagctaaa gaattgaacg taagcgaagt acacggaacc aaaatcgtgg atcttcagtt
    12421 ataatcttat aaacaaaaaa acatttgcaa caggcataat gcttattgca aatgtttttg
    12481 tttttttaca taaaatagcc cataaataaa gaagcaatat tgaaataaat cagtacactc
    12541 gtcaaatcgc tcaaagtcgt gataaaggga ccgcttgcta ctgctggatc aaatcctaga
    12601 cgatccatta gcatcgggat taaactacct gcaagattcg ccactgtgat tgcaaatagc
    12661 atagccatcc ctatgacaaa ccccaagata aaattatgtt tccaaaaacc tacgattaga
    12721 aaaatcgtga tccccgtaac agctcctgtg acaacgccgg tcaagacttc agccaaaaca
    12781 agtcgaacaa atccatgatc tttctcatca gaaatcgcaa gtcgacgaac agccacagct
    12841 agcgactggg ttccagcatt accggcagtt cctgtaatca gcgaaatgaa gactgctaaa
    12901 atactcgctt cacttaccaa gtcttcatag tgactgatta aagaagcggt cgacatacct
    12961 aaaaacaaca gagtgatcag ccaaggcaaa cgtcgagacg ctgcctttac tggattttca
    13021 ttaatttctt ccacattgac cccggcaagt cctgaataat cgctagcagc ttcatcatca
    13081 ataacgtcga tgatatcatc gactgtgacg attcctaaca aatgatcatc ataatcggta
    13141 acaggaagag ctaagaagtc ataatctcta aatgtttgtg ccacatcttc ttggtcgtcg
    13201 cctacatgaa ctgaaagcac tcgttcgctc atcagatcag aaatcattgc gtcgtcatca
    13261 ttcacgatta ggtcgcgcag agaaataacc cctactagct gaccttcttg attaacgaca
    13321 tagatgtaat agattgtttc agctacatct gcttcgttct tcagcacgta catcgcagag
    13381 cgaacagttt gattggctac gatcgaaaca aattcagttg tcatgatcgc gccagccgtt
    13441 tcatcctcgt aatggagaag ttccttgatt tcactggcat cgtctgccga catcaaactt
    13501 aaatatttag caatttgctt tttatccagc gtattaagca agtcaacagc attatctgta
    13561 tacatctcag aaagcatatc agctgcatag ctaggacgca tttcatctag gtattctttc
    13621 atatgttcgt tgtcttcttc aatcacatcg aacatatccg ctaattcttt aggcgacaga
    13681 taggaataga tcagctgcct gttctcttct gtaagagatt ggtagaattg cccctgctca
    13741 tagatatgca tctctaaaaa actgtttctg aatgcctgaa tattattttc ccttaaatct
    13801 tgagtcaact gggcaaatct ttcctctaat tcttgatttt catccaaacc aatctcacct
    13861 cgccttattc ttcaaaccgt ctcacaactg cctccatgtc tgcggctaaa ggctgcttga
    13921 actccagcaa ctcttctgta aatggatggt aaaaacgcag atcataacaa tgaagggctt
    13981 gtctttttat tcccagatcc attcgcccgc catacatatc atcaccgagc agggcacagc
    14041 caattgcgga aaaatgaact cttatttgat gtgtccttcc tgtatgcagc tggatatcaa
    14101 ctaacgcgat ttctgttagt cgcttctcta tccagtactc agtctgtgcc gaacgtcctt
    14161 catcagagac cattcgcttc aatagcgaag aataatctcg ggcgatcggc aaatcgatat
    14221 gtccatgtgc cgataaatct gaaagccttc cactgactaa ggcctgatat tttttcacaa
    14281 acttcttttc acgaagttgc atatctaatt ttgcgtgcgc aaatccatgc tttgcaaaaa
    14341 gcatcaaacc agatgtatcc cgatctagtc gcgtcaccac atggattact tggttttcgt
    14401 aaccttgttt tttataataa gcctttacac gattagccat tgtcccattt ggatggtatt
    14461 gggcaggaat agacgaaacg cctgctggtt tatttactac cagaacgtgt tcatcttcat
    14521 acacgatatc gatcggtgtt tcatccaaaa gtacggtttc atgttctcct tcagcaggaa
    14581 tcacaatggt taccttatca tccttagcta gagaaaacaa gacattttct acttgatcat
    14641 taactttgat ttgaccgcct tgaaatttta ttttcgctaa cagacccttt gagatccctt
    14701 tttcttttaa aaaatatttt acttgctgag aatgatcttt ctcataatcc catgtaaact
    14761 gcatatcaat cctcactaat aaaggcatct ttcacacgat gccaaaaatg catatgtcga
    14821 taagaagcaa aatggatccg ttcttctgct attcgataat aaattgagcg gatatttgct
    14881 tttgcgacat tcaattgatc aaccgttaca agataatctt ctgtattttc caattttatt
    14941 tctagccatt catcatgggc aatcacgatc ggagaaccca aagtacgaaa cactcgatta
    15001 ttcagtgaag caatctctgc caactgaaag gcattgatac taggatggat cacagcaccg
    15061 ccgacacttt tattatatgc tgtcgaacca gtaggcgtag aaatcgtcaa tccatctcct
    15121 ctaaaacttt caaacagctc gtttttgatg tatacatcgg ctaccatcgt acgattggcc
    15181 cgtttaatcg ttgattcatt cagtgctaaa aaatgctggt ccggtgtttc atccaaataa
    15241 ctgatccgaa catccagcaa gggataacta acactttgtt cccggttcgt tcgtaagctg
    15301 tctactagtt cttccagctc gtaatcccgc caatccgtgt aaaaccctaa atgacctgta
    15361 tgcacgccca agaaacgcac atcattcaga caatgactgt agtgatggaa cgccgacaat
    15421 aatgtgccgt ctccgccgac tgagatgacc aattctggct catgatcatc tcgttgaatc
    15481 cctgcttgtt ctaaaagtgc gttcaaacgt tctgtcactt ctatagtctt tgcttcttta
    15541 ttatgaacga tcgctacttt catcccctgc ctcctctctt tcgctttctt tcttcaaaag
    15601 cgttcgatag tagacatcat ctgattttcc tcttccatgg gagaataacc gctgtgcttc
    15661 ttggatttct tcacggatac tggacatctc ttcatccaac tgataggcag cttctgccgc
    15721 acgctgaagt cgttcactca tttcttctgg aaaaacgcct tgatatttat aattcagcga
    15781 atgctcaatc gttgcccaga aattcatcgc aagagtccga atctggattt ccgccaaaat
    15841 tttcttctca ccgctgatca gctgtacagg atactcgatc accagatgat aagaacggta
    15901 gccacttgct tttttatttg tgatgtaatc tcgttcttgg agaactttca tgtcttttct
    15961 tttacggatg atctcaacaa cttgatggat atcttctaca aactgacaca tgatccttaa
    16021 cccggcaata tcagacattt cttcttctaa acgtcccatt ggaatatgcc gtaattgtga
    16081 ttttgttaca atactatcga ctggcttgac tctcccagtg acaaattcaa tcgggacatg
    16141 tcgattttgt tcacggaact gtttgcggat cccccgcagt ttcactttca attctgatac
    16201 agtttgttca tatggtgcta aaaatgcttc ccaatctcgt tccatagctg atacctctac
    16261 ttcattaaca ttcattgttt attctaccat atatgcaaag ttttttcgta ctcttgattt
    16321 atgcattgac caaaaaaaga aaattaggca aaatagacaa aaaggagtcg atcaaatgac
    16381 acaaatgctt gaaatcgaat ataaatctat gctgacaaag caagagtacg agatattcat
    16441 cgctcactat caattaacag ataaagattt ccgtgtacag actaatattt atttcgatac
    16501 cgttgatgaa cagctgaaga aacaaaattg tggcctacgg atccgtttcg ttggtcatca
    16561 agctgaatat acattaaaaa cacctgctga aaaagggcgt ctcgaaacaa cggatacctt
    16621 tataacagaa gaagcagaag catttatctt cgaaaaacgt ctgcctcgtt caggcgcagt
    16681 ttttcaaaaa ctttctgatt tgaatatcga tcctgcttca ttaatccaaa ccggaaagct
    16741 tacaacaaaa cgggctgaat tccctattga agaaggactt ttagctattg atgaaagctg
    16801 gggagacaat ctacacgatt ttgaactgga actagaggta ggggatgcat ctgtgggaaa
    16861 aattgctttt ataaattttt taaaacgttt ttctttacct tatcgtcctg ctaagaacaa
    16921 gatccaacgt atgcttgaag ctaaaaatta gaaacctttc attttcacaa ggcacttgag
    16981 ttcatttttt tacttttttc tgttaagtta taggatgtga aagaatctag tcactagttg
    17041 tatagaatct attttggggg agattcatat gatcgaaatc tatttatttg ttaatccttt
    17101 aggggaatac tgtttcgaaa tggaacaaca gttattacaa tttatcgaag aagagtacgg
    17161 gacaccatca aaagaaaaga tgcaatttcg ctttcttcct ttggtcaatt tgcagacaat
    17221 tggggatgtc atgcaacaaa aaggaatttc tcagaacaat cttgaggaac gtaatcactt
    17281 attctcaatg acttattctg cagcattgga ctgcaaagcg gcgcagtttc aaggaaagaa
    17341 aaaaggccgc cgttttttga tcaaattgca agaagcagtc ggctgtaatg acataccata
    17401 ttctcaagaa cttgctgaat ccatatttga agaaatcggc ggcgatttga atatgtttaa
    17461 agaagatcgt cagtctgact tcgttaaaga agtattcttt tctgatcaaa aaattgctcg
    17521 ggaaatgggc atcacaaaac acccttctgc agtcgtttac aattatgcct gcgaccgtga
    17581 ttttggtgtt ttagtagaag taaaaggtac aagtagcctt tggagaatca cagagctgtg
    17641 taaaacaaca gataactcat atcaagtttt taatggtgaa catttacata caaatgattc
    17701 tcagttccat tcaagaacta gccatttaca cttgctttct aattaaaaaa atacgtcgac
    17761 tcccttacaa gagagccgac gtattttttt cgtttctatt cgagaaaatc aaaaatagaa
    17821 agccttataa acgatcgatc aattcttcta attcatttag acgttgttca aacatcgaca
    17881 ttgcatcttc aatatatgca gcctgtgtca tatcaactcc tgcttttttc attacttcca
    17941 caggataatc gctgttacct gcttttaggt aagccaagta gttctctagt gcctctggtt
    18001 cctggtttag tattttcttc gccagtgctg aagcagcaga aaagccagtc gagtattgga
    18061 aaacatagta attataataa aaatgcggta tccgtgacca ttcgaattta atttctgggt
    18121 cttcttcgac tgctggacca taatattttg cattcaattt accataacta tcacttaggt
    18181 attcactagt caatggcaca cctttttcat cttccgtatg catgaaatgc tcgaattcag
    18241 cgaattgtgt ctggcggaaa actgttcctt taaacccatc caagtaatgg ttgagtacat
    18301 aagcccgtac acgagggtct ttttctgttt ccaataaata ctccgttagg atattttcat
    18361 tcgttgtcga agcaatttct gctaaaaaga tggagtagtc gccgtacaca taaggctggt
    18421 tcgaacgagt gaaataacta tgaacactat gtcccatttc gtggacaagc gtaaatagct
    18481 gatccagcgt atcatgccaa ttcaataaaa tatatggatt tgtgtcatag cttcccgaag
    18541 aataagcatc gcttcgtttc cctttatttt cgacaacatc gatccaacgt tcagagaatg
    18601 ctttttctac gatggccatg tattcttcac ccattggttt cagtgcttct aaagcttttt
    18661 cttttgcttc ttcgtacgta aaggtaattg gagcttcccc taagaccggt gtataaagat
    18721 catacatgtg cagtttttcc acttctaata aacgtttccg taattccatg tatcgatgta
    18781 acaaaggcaa atgcttgttt accacgtcta ccaaagtatc gtatacactt tcaggaatat
    18841 gattattgct caaagacgct tctctggcag agctgtaatt acggactttc gctttaaaat
    18901 tatgtccttt tatatgtgtg cctaaagtag aagcaaatgt atttctaaat tgttcgtaaa
    18961 cactgtacaa tcccttaaat gctgcttcgc gcacccttcg atccgtgctt tctagcaact
    19021 gaccatacac gccatgagat aattggacta tttcaccatt ttctccttca atcgttggaa
    19081 aaactagatc tgcattattc aaaacagcaa atgtatcact tgaagcatca aagatttcac
    19141 ctgctccagc aagaagagat tcctgctcag cagataagac atgggctcga ttatctacta
    19201 tttgctggat ataatggcga tagacttcca attttggttc ttctttgaaa tattgccaaa
    19261 tctggtcatc tgacaattgc aatatttctg gttcaaacca ggaaacggct tcactaactt
    19321 tcgaaaacag actgcttgct cttgcataaa gcgcttggta atctgtattt ccagtgtctt
    19381 gatcgttttt aaggtgcgca tatacataaa tgacttcagt ttggcgataa accctcaata
    19441 cgaattcaat cgcatttaaa aattgagaag cgccttgatc aagtgttcct ttgtgttttt
    19501 cagattgttt taactcttct gataattcca aatatttctc atcaaactct tggtcgcttg
    19561 aaaagatctt ggtcaagtcc caagttaaat tttcaggcaa ttcttctcgt tttggcaact
    19621 gctttacttc catgaaatcc actccttttc ttcccttagc tctatctatc aaaatagatc
    19681 atagtctaac atccacatgt cacagcatct aacttttcta ctcattcatg tgtctgcttc
    19741 catcaatttt agcataacta aatcttgttt tacatagaaa accgattcct agcctatttt
    19801 tatacttact attttttcaa agaagacaag tgtatgcatt cgttaaaaaa ctgctgatag
    19861 accatctctt catcaatcat aggaaaaagc cattcccttt tatgttcttt tatttggcaa
    19921 agccatgagt tgaaatcagg aactttctgg ttttccaagg cttcttcgta caacattcga
    19981 taaagaaaaa cctgttcttg taaataaaag aaaaaccgac ttttttgata gatccacgat
    20041 gatagatgca aaagatgttt atgacgcaag tagcattgtt cttgtatgga tactgtattc
    20101 ttttgcttgt gaataagttg gcgtgtcagc catttcttcc catcttcact cactttttct
    20161 atttttttca tttggttcat tttaatttcg ttactagtga atatttcttt aaggtcccta
    20221 ctagaaaaag tccagcagct actgcaaaag gaaatatgcc ctgatacagt ctctgtaata
    20281 tgatgataca aataaagctt ttgtttcttg agatcagctt tccagcaatg cactcctctt
    20341 tttctattat agtagcagca acttttttct atcaatgaga attgcgattg tccaagaaaa
    20401 gaattaccaa gtatccacca tacttggtaa ctgttattca aataattttg ggttctttca
    20461 ataaatctct ggtgagacag tctagaacat tggatttcta ttgctagatt cgccaacaaa
    20521 atatccggcc tttgccgcaa cctcggcaaa taggcttcca aaaatactgg ttctactgac
    20581 tgtcccagcc aatccatgaa gcattccttt aactgtaagt gctcttttga ttctctttca
    20641 ctaaagaccg aacagttatc tgatttttga tgggcaaaat gagcacattt tttctttccc
    20701 tttttcaaaa tcactctttt tcgacagcca cgacaataat atttctttcc tttttctgca
    20761 tgtttagcaa aacagcactt attatattca ttcattgcaa gcagcatttt tctccctctg
    20821 aacttattta cgtctaagaa tttatttaca cgaaaaaaga gggcgtgaaa aagctattct
    20881 gcatcaagta ataagaacag ctaaacaaaa atagttccca atatttttcg tttggttggg
    20941 cttgttaccg cagatgcaag cttttgaaca catttatacg aaaaaaagac tgcgacacaa
    21001 tttttgtcac agtctcttac tataatgctg atttatacaa aataaaattt cgttcgctcg
    21061 atagcattac gcgccatcag acattgtcca tattcattca atgtttctgg tgtacgatcg
    21121 ctcttttcag caaactccaa aattcgagca atctgattct ctacatccgc ttggttgaga
    21181 ttttccatcc agaagtgaac gactaggtaa tagcgctctt cgaataaata caaatcagtc
    21241 caagcagact ctagttcgac atctgttgca agttggatca cttgttcaaa atcttcaaaa
    21301 gcaaaagttg cttgtctttc ctgttcttct agggcgcttc cagataattc ttcctccgga
    21361 tcgctgccaa taatctgctg acgaagaaag tcactcacat cttctgaatc aacagtagaa
    21421 tcttctgatt gagaaatttc ttcatccgga agatttttac tgataaacag ttctaatccg
    21481 tctccttttg gcaaaacttg gaaagtgaca gcttcgcttc ctcggaactc ctcttcgata
    21541 tcaacttctt ccaaaatact ataaaagaaa ttttcgattt ctttgtgatt gccaagtaaa
    21601 tccaaaaatg aaacgccccg ttcctctaaa tcttcatgac caattagtac acggatcgta
    21661 ttttcattaa tatgttccat ttccattttg ctacacctca ctttgccaca atgtttaata
    21721 tagattcatt gtagcgaaat cagcttaaat gtaaagaaaa aaaatctcgc tgttaagcga
    21781 atttaataaa accagcggat ttttcaacct ttcaagctat cttatttaac tataacattt
    21841 ttggcagacg aatactatat agccgacttt tcagctaatt ttccctctct atttttcgtg
    21901 caatcatcat cttctactac ctttttatac atctaatcaa gaatactttt tgtaaaaaca
    21961 aaaaaacttg gaaaaactat ctttgtaata gt
//
LOCUS       DANKHL010000028        21898 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000028 DANKHL010000000
VERSION     DANKHL010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.030"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            6..245
                     /locus_tag="PW101_000733"
     CDS             6..245
                     /locus_tag="PW101_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513157.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            312..521
                     /locus_tag="PW101_000734"
     CDS             312..521
                     /locus_tag="PW101_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513158.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            581..1252
                     /locus_tag="PW101_000735"
     CDS             581..1252
                     /locus_tag="PW101_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDL2513159.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVLAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1305..3281
                     /locus_tag="PW101_000736"
     CDS             1305..3281
                     /locus_tag="PW101_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL2513160.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDITQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            3296..4048
                     /locus_tag="PW101_000737"
     CDS             3296..4048
                     /locus_tag="PW101_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2513161.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            4064..4819
                     /locus_tag="PW101_000738"
     CDS             4064..4819
                     /locus_tag="PW101_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513162.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNEETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            4832..5092
                     /locus_tag="PW101_000739"
     CDS             4832..5092
                     /locus_tag="PW101_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513163.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            5089..7179
                     /locus_tag="PW101_000740"
     CDS             5089..7179
                     /locus_tag="PW101_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL2513164.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            7206..7670
                     /locus_tag="PW101_000741"
     CDS             7206..7670
                     /locus_tag="PW101_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513165.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            7667..7909
                     /locus_tag="PW101_000742"
     CDS             7667..7909
                     /locus_tag="PW101_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HDL2513166.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            7927..8229
                     /locus_tag="PW101_000743"
     CDS             7927..8229
                     /locus_tag="PW101_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513167.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            8299..10362
                     /locus_tag="PW101_000744"
     CDS             8299..10362
                     /locus_tag="PW101_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513168.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            10362..12974
                     /locus_tag="PW101_000745"
     CDS             10362..12974
                     /locus_tag="PW101_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDL2513169.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            12971..15502
                     /locus_tag="PW101_000746"
     CDS             12971..15502
                     /locus_tag="PW101_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513170.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKEND
                     QSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            15552..15884
                     /locus_tag="PW101_000747"
     CDS             15552..15884
                     /locus_tag="PW101_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513171.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            15885..16478
                     /locus_tag="PW101_000748"
     CDS             15885..16478
                     /locus_tag="PW101_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513172.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            16641..18665
                     /locus_tag="PW101_000749"
     CDS             16641..18665
                     /locus_tag="PW101_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDL2513173.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            18665..19864
                     /locus_tag="PW101_000750"
     CDS             18665..19864
                     /locus_tag="PW101_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL2513174.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            19880..20524
                     /locus_tag="PW101_000751"
     CDS             19880..20524
                     /locus_tag="PW101_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513175.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            20535..21341
                     /locus_tag="PW101_000752"
     CDS             20535..21341
                     /locus_tag="PW101_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513176.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            21363..21593
                     /locus_tag="PW101_000753"
     CDS             21363..21593
                     /locus_tag="PW101_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513177.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
BASE COUNT         8098 a         3442 c         4501 g         5857 t
ORIGIN      
        1 agtagatggc gcgaccagtt ttagaggcga tggatatgga tactttgaca aaaggaaggt
       61 atgtacagct gttagctcgt ggttattcac ctaaagaagt attcaaagcc ttatctaaag
      121 gaacagatat ggaaaaagaa agattgcgaa aagaattcga ttattggaga acccataatg
      181 gaatcaagga tttgaaacca gcacggccag taattaaacg aaagaaaaag ataacccaag
      241 actgaagtgt agagcgtatg tgatcatagg cttttttatt tttgcaaaaa acaaggaagg
      301 taggaaaact catgaattat gaagaaatcg aaaacagaaa aaaagtgtcg aaagaaatgg
      361 aagaaaagtt actaaaaacg atgaagcaaa aacaccttaa aagattatct gtcgcgcaat
      421 atatcaatga tatgcagtta acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa
      481 actttgaaca gttgagacga acttatagaa ttcattttta aaactttttc tatggaaatg
      541 agaaaaagtt tttttgtttt gtctgaaagg aggcagatgg atgaaaaaat ggttatttgg
      601 ttttttagga gtcactttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa
      661 acatgaagga gaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa
      721 tgcacatgca aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg
      781 gcaaatgttg tccatacaaa aagaagcaat cgaaatgggc gtcaataaac aagtacttgc
      841 acaaattcag attcctgcac ttggtttagc attgccaatt tttaaagggg caaatcaata
      901 cactttgagt cttggagcgg ctacttattt ttatgaagat gccgaaatgg gcaaaggaaa
      961 ctatgtccta gcaggtcata acatggaaat gccaggtgtt ttattttctg atattcagaa
     1021 gttgtcatta ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt
     1081 aacgcgtaaa tttatcgtac ctgagtactt taaattaatc gatggcgttc ctgaagagaa
     1141 ctctttcttg tctttgccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt
     1201 ctatacgtca caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaaggttt
     1261 taatcaacgc gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt
     1321 atgtaaaaaa agctggaatg cttattggaa tgttaggaat cattgcaggc tgctttcagg
     1381 gatatcgagc atatgcagaa gatatcacgc aaaaaacgcc accagaaaag gtcaatatca
     1441 cggttcataa attaatgtac gatcaaggaa cgcaattgaa tgttgatatt gacgggatca
     1501 aaaacgatgg ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag
     1561 attatggtga tgttgagttt acgttaggaa atattacgga tcaagtatta ccgactgaag
     1621 attctgatct aaccaacgcg aaagtggacg aaatcgttaa agatgtagaa gacaagggat
     1681 ctaacagtga gtacgtcaag aatgcaacaa acaaaattac ttcagctgta gatgaaaatg
     1741 gtgagattac ttttgctgat caaccagcct atgtcaattc caaagggaat gtatatgtgg
     1801 tttatgaaag taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga
     1861 ttgcgccaat gactgataat acaggttcag gttttttaaa agacattcac ttatatccta
     1921 aaaatattgt ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcacagctc
     1981 aaagcaaaca aacaccttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa
     2041 aaggaacaaa attaggagat cttgtatcgg atgatcaagg aaagctgact gctactgatt
     2101 tgacgttagg aaaatactat tttgtggaag ttccttcaga agttgtagtg ggatcagata
     2161 aagaaccaac agcagatcaa tatttattag gcgcagacgc tcgcaacgat gcgcacaata
     2221 aattaacctt tgaaattacg aatgatggcg tgacaagtga cttaaaagct tcatacgtaa
     2281 actataaagc accagtgatc gataaaacag taacaaatgg aacaggacaa gaacattctt
     2341 tccaaattgg tgacgcagta aattatcaag gaacaattca tatcccaaca gacattgcag
     2401 gcggagcaga cggtatcaca gtaaacggag tgaaaagcga aacttctcct tacagtgtat
     2461 ttaaatgggg ggatacagca ggacaaggac tttcttatgt cgctgctaaa gcaaatatca
     2521 aagtaacaaa taaagatgga tctgtggtat taaaagaaaa tacagattat aaaatccaaa
     2581 attcagaaaa tggctttgtg atcgatttta tcgtgaataa tggccaagta agtgatactg
     2641 tagcaagttt acacggacaa gatcttcaaa tgacttataa catgtatgta aatgacagcg
     2701 cagctgttgc caatccatta acaaatagcg tggatttcgt ttataacaac aatccattta
     2761 atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt gacttatggt gcaaaattct
     2821 taaaagtcga tagcggacta tttggaacag gaatcaaagc aacaccgctt gaaggcgcag
     2881 aatttgctgc taaaaacgca gaaggaaaat attatggtgg attagttgat acggataaag
     2941 acggtgtcaa agaagcagta tgggtagacg atgtagcaaa tgcagcgatc ctaaaatcag
     3001 ataaagaagg tcactttgaa atcactggtt taactgaagg tgaatattca ctggaagaaa
     3061 caaaagcgcc tgaaaactat caaaaattaa cgaaagagat ctctttcaaa gtagacaaag
     3121 attcatacaa agaagaaaac cgaatcacga tcaaaaacaa tcaaaaagct tccgtaccaa
     3181 tgaccggtag caacggtttt caaacgtatg tattaatctc ttgcttgttg ctaggagcag
     3241 gggcactttc tgcagttgtt tactttaaga agaaagccta gaaggatcaa aaccaatgat
     3301 tatcaggaag cctaagaaaa agcgaatcat gggtaaatgg atcattgctt tctggttact
     3361 ttcagctgta ggtgtcttac ttttgatgcc tgctgaagcg agcgtggcga aataccaaca
     3421 aaatcagcag attgccgcaa tcgatcgaac aggaacggca gcagaaacgg attcttcttt
     3481 ggatgttgct aagattgagt taggcgatcc tgttggtatt cttacaattc taagtatttc
     3541 tttaaaacta ccaatttatg acggaacgtc cgacaaaatt ttagaaaatg gtgtaggtat
     3601 tacggaagga actggtgata ttacaggagg aaacggtaaa aatcctttaa tcgcaggtca
     3661 tagtggactt tataaagata atctttttga tgatttacct tcagtaaaaa agggagaaaa
     3721 gttttatatc aaagttgatg gtgagcagca cgcttatcaa atcgatcgga ttgaggaagt
     3781 gcaaaaagac gaactacaaa gaaactttgt cacttactta gaatcaaatc ccaatgaaga
     3841 tcgagtaacg ctaatgacat gtacgcctaa ggggatcaac acacaccgtt ttctagtgta
     3901 tgggaagcga gtaaccttta caaaatcaga attaaaagat gaagaaaata agaaacaaaa
     3961 attatcttgg aaatggcttt taggaagtac tgtttttctt tcagtcatga tcattggttc
     4021 gctatttgtt tataaaaaga aaaaataaag gagggtaacc aacatgagaa atagaaaatg
     4081 gctatttgtt tgtgcagccg ctcttttttc tttcactgcc tacacacagt tgacgtttgc
     4141 agacagacaa gaaagcacgg atagcacaga acaaacaatc caaacgtcag atagcagcac
     4201 gattcaaagc agcgatgaaa ccgtcgattc gtcaacagtt gattcttcag aagaacctaa
     4261 aattgctgaa gacgatcaaa tagtcaaaca gaccattcac gttcaaaaga ttatttcttc
     4321 agaacaatta gacaaccagg ggcatgaaaa acaagaagga gtaaatggcg cgaagttttc
     4381 tgtttatgat gtatcggaca ttttacaaaa gatggatgtt aaggatctca ctactgacca
     4441 aattgaatcc caattaaaaa atcgagtgaa aaaactatcc agtgatcaat tgaagctagt
     4501 ttctaatgaa gaaacaaaga caattgatca acaaacaggt gtctttgaat tctcggtaga
     4561 agtacaggca aatcagaaac aagcttatta tattgtcaat gaatcaagtc ctgaaaacat
     4621 ttctaatagc gaggatattt tgctattaac acctgtttct gataaaaacg gtgaattctt
     4681 aaaggatgtg tggatctatc ctaaatcaga agcttcgcaa cctaaagaag aagtaaaaaa
     4741 aatcgtttct actggcgtaa agaaaaactt ctttgagaat tgttgggatt ttgttactca
     4801 cttgttttct agagattaag gaagaaataa aatgaaactg atcaaattat tattattcaa
     4861 tttttcttgt ttaaccgcag tctttttgta ctgcggttta ttattttcta aactatctat
     4921 tttgccaagc taccaactat acctaatgag gattttaaac ataccagctg cttccatgcc
     4981 gctagatatt atggaaaagc tcattaggaa tccattattt actacgatca atttgatcgt
     5041 tattgtcgga atttcactag tcatttataa aaaagagggg atgaagggat gaattttaaa
     5101 cactcgtgga caagacggtt aaaatatgtt tttcccataa tgatgatgtt aggtacattg
     5161 tttgggttaa aaacagccaa tgtctttgct gaaaaagtta ttgtcgatcc gtcaaatcca
     5221 atccagaacg taaagaataa ttttattatt ccaaaagatg ttcctaatgc aaaaacaaat
     5281 attactgtaa acggagcgtc aaaagtttac aaatatcatg ttgatgccaa caaaggtggt
     5341 tttacagacg actacgtagg attagtggag ggaaacaaaa atatacgcta tccttctttc
     5401 caaaaagaat acggtgaaac aaaccttgtt ttagaaggcg taacaaccaa caccaaagtc
     5461 aatattgact atggaaaaat cggtacgtat aacgggaaaa aagtcaatat caagctagtt
     5521 ctatctaata ttcacttata ctcggatacg ttgccgtgga atctactaga taacaactat
     5581 acaaaaactc attttcgaga cgatggctat aaaaatacga atggtgcaat gagtaaatct
     5641 aaaaaaagaa cagtactttg gatctcagac aatcttttct caggaattgt ttaccattca
     5701 acacaaatga atgtgcagct agtagccaca tatgaggacg gtagccccgt tcaattttca
     5761 ggagatacat ttatcagttt taactctttg aaccctgcag gaggaaaaag cacggatctt
     5821 aaaggagaat atgcccacta tgacaaaatg aatacgacag attggtatgt cagaaaagat
     5881 acggttcttt cagaatttaa atctttctac aacaatctga acgtggtagg tggccaccca
     5941 ggaggaagct ctaagctgac acaagcggat aatgatttca ataatttgca tgataaatta
     6001 ggagatccga agtttggtca aggtactgtg tcctttaaaa tatctgaagc aaatccgacg
     6061 tttgtgattg gttcttctaa tgttcaaacc tggtttactt tatcttctgc gacaatcttt
     6121 agtgttgttc ctgatcaacc tgaaaaaaca ggggttgata aaaatggaaa taacgtgaac
     6181 gataaaatgt tgcaagtagg agacacgatt caatatcgaa tcaaacagaa ggtgaatcga
     6241 ttaggagtgg atttactagc ggtttacgat cgatttgagt taatcgataa tttaccaaaa
     6301 gaagtaaatt acgtcgatgc acacgttgaa agtggtacaa ataaaaaatt cgatgtatct
     6361 ggtgaagtga cctatgacaa aacaaaacac caggtaaaat atgcagcgaa agctgataca
     6421 ttaaaaaatc gaatgaagta taacggcgaa acttacgaat tagtgatcaa tgtaaaagtc
     6481 aatgaattgg ccaatcaaaa tagtgtagcc aaaaatcagg gaacgtcgat cattaataaa
     6541 gtagaaaagg atacaaatat cattaccgtt tatttcccta aaattccagt gaaagaagta
     6601 caacaaaatg gaaaagatgt aaacggacga aatgacggta agaagggtac gccgacagct
     6661 cctttaaacg caggatctga agtacaatat ctagtcacac aaaaatggca cacaaaaggc
     6721 gtagacgctg cgtcagatca ctacaaacaa tttagtattc aagatccaat tgaagctcgt
     6781 ttgacatata aagaaggttc tgcgcaagta atagataaga gtaaaggaaa agatattact
     6841 tctgaaggca cactaaccta tgatagcaat tcaagaacgc tgaagtggga agcttccgct
     6901 gacttcttaa gtaacaatct tttagatgga cgagaaattc agctgatatt tacagctaaa
     6961 actccattac aatcagaaaa aaatattgat aaccaagccg tagtagcagt agataatgtt
     7021 tctaataaaa caaatgttgt gacaattggg gttgatccta acttgccaca ggtcattgtt
     7081 cctaaaacag gttctacaca tttagtaacg atttcagcgg tcagcttact attacttgtg
     7141 ttagcaactt ttagttatgc ggttctgaga tacatttaaa ataaaaaaac gaaagaatga
     7201 ataaaatgaa aaagttaata atagtagtta cttccttgat tgccatgatt gttattttag
     7261 tcggatgtag taatgaaaaa aataaacaaa ctaatcaaga taatggcgta ttgaaatcag
     7321 gaactatgtg gaaagaagaa gtaggtggtc ttgtttataa tttgaaaatt atagacgaga
     7381 ctacctggga atactctgag agtgtatggc atccagatcc tgtacaaata acagtcaaga
     7441 ggcaaaaaga ctataaaggt ttagaaagat acaaaatagt agattcagct ggtgttaggg
     7501 aatttataaa caaatctgat tctttattta ttgttgttcc ctatgagaag aatggcgtaa
     7561 aaaaaataat ttttcttgga tctagcaagg atgaaaagcg aacgaaagag aaattgattc
     7621 acgatggatc acaatccaat aaatataagt tacaaaagac tagcgagtga ttgctagtct
     7681 ttttatttgt cctgaaggag gaatcacagt ggcaaaagct aaaacgccat ttgaaaaagc
     7741 cgcgtctaaa atcttagaac gccgcggtgt atctgaaaca cagtggaaac aagaagtcct
     7801 tgaaaaagca caatttgacc tattacgggg aaaagacaag gacttagaag gatatatcta
     7861 tcaacgtgaa cgtgaaaagc ttgttttcga tgaaattatt aaacactaaa aggagcgaac
     7921 aattttatgt ttgaatcaat tacaatgaat tttttaacac aaccgaaagt agtagctgcg
     7981 gttgatattt ttagagcatt acaaactgcc gcaactagtt taaaaaacaa tctgatgatt
     8041 tttgctgctg ctgcggccgt tgcttccatt gttattgggg gtcttttctt cctatttgga
     8101 cgaagaggag ctgaaaatgg gaagatgcac gtaggctatg tattgatggg ggtaggagtt
     8161 attttagcag cagcttctat tgtggtttcg ttatttggat tgtttggaca aacacctcaa
     8221 ggattataag aaagtaaaaa agtaggagta agaagtagtc gatacttttt actccttttt
     8281 tatgaaagga gaatactcat gtactttacg attgcaggca ataccaaaac agataaagag
     8341 ttaataaaaa aagcaacgaa ggaagtagga aagcgtactt cttttttacc gaaaacaatt
     8401 aaatattctg tcattgaaac ggaagaacag ctacatgtgt tagggaaagt actggaacac
     8461 gaaagtcgat cttctgttaa gttgactctc acgttacatg acacagaagg aaaaatagag
     8521 aaagctggta cagaatacgt ttttggccca ctggattatc aaatttcaga aggtttctat
     8581 cctacattga tggatttaat ccgccttgat gtgtttaaag aaacggatga aactatgagt
     8641 gtggaagagt cttatcgaaa cgaaaaactt ttacaagttt tagaagaaga gttagagagc
     8701 gaccaaagaa aagtgaatca agggagtgaa gtcgaagtaa atgaagaagt accatgggaa
     8761 cgaccaatat ttccaaagaa ggagtcaaaa gaaaaagaga cagtctatgt tgacgaacaa
     8821 gctgatattc cgactcagaa atcgatattt gaggaagaga acgatgtgat gggtcaattg
     8881 gaggtagatc cctttgaaga aacaatggag gatgaagaga cggccgaagt agaagttgat
     8941 ccagttgaag cgaatgaaga gtcaacatgg actgaagggg attctcagga atatattgaa
     9001 ccacttatca gtgaagaagt agaaaaaaac gaaatagaga tacaaaaggc aactgtattt
     9061 gattatattt tggatcaata tccgccagag catgaatgga tgaaagaaaa aaacaaatta
     9121 tttttaaatg agctttattc gaaatatcaa ttaccccaaa cctatcaagc atttttagag
     9181 agtaaagaag aattaatctc acagggtaaa ggcacacttt tagaacagtt agagtctgtc
     9241 caatcaacaa attggcatga gcttgcacaa gaagatttat tagccacctt tgaagaacgt
     9301 caacaagaaa cagatcaaga aattaatgag tatgcgacgg aacagtcgtc taattgggag
     9361 aaagcaaaag aggaactaaa taaagaagaa cagcgagtga ttgaagaaga agtacaaaaa
     9421 attaaagagc gatacgaaaa gaaacgcgac aatgcgtatt ccttgtgtgt agcaaagatc
     9481 aataatttta ctcagacgaa tcaagaagcg ttatcgaaag aaaaagaaaa cttgttaaaa
     9541 gaacgtatta aagaacgaaa agaagaattt taccgaggtt tgcgtacaga taagctaaag
     9601 attagcaaag aattaaatga acaactgaat aagctatatc aaacaacgac tgaagttctt
     9661 atggaaaaat acgaaaagat ccagcaagaa ttacaagtgc aaattccaaa atggaaaaaa
     9721 gagtatgaaa ttcaacaaca agaattgaaa gaacaagaag aaaaacaacg gcaaaaagaa
     9781 aaagagcgcg aacaattaga actaaaaaaa cgtgaacagg atcttgcgga acaacaaatg
     9841 gcaatagaaa aacaacggtt aaaccaagaa aaggaaaaag aagcagctgc tcgtaaggaa
     9901 cgtcaagaac aaatggatac attgcgacaa gctcagttaa attatctagc acaacctcaa
     9961 ccaacgcctt cagtagctgc tgttcaaacc ccaatgaaca accaaacaaa tctaactcct
    10021 tcaccacagc gttcctttaa cggatggatg gtgggatgta ttgcaagttt aagcttgctt
    10081 ctaggtggag gaacagtatt tgcttttaat catatcaatc aatcttcaca ggctgattct
    10141 gtggccacaa cgacgtcaat caaggaagaa gcccaagaac aagcaagaaa agagtatgaa
    10201 caacaattag aagaattatt gaatcgtacc aatcagtcaa caacagagtc aagcatggaa
    10261 aatagcaaag agagtgattc tagtaaggct aaagaaggat caagcgattc aactgtacaa
    10321 tcaaacactt ccgaaagttc agaatcaaat gaggtgaaat aatgcgtgtt agagaaacaa
    10381 cgaagcgaaa agacaaacga ttcaatttag aaaagaatag gtatctttct catttctatc
    10441 gctatactcc ctcaggagaa gagctaccag aaaaacatga caaacgcatt tcgaaaaaga
    10501 ttttttatag tggacttgta tacctccctt tagagtattt agtaacaaga gtaccagaag
    10561 ttttcaaccg attggaaatt gttaagggaa gcttacaagg aataaatctg tttcaattac
    10621 aggaaatgcc acaggtcttt cattttgatt cagctggttt ttggaatcca tttactccta
    10681 caactagttt gggtacacgt ttagcaattt cgacagtttg tgtggttcca gctgtactta
    10741 tgggattgaa aatggacatg tggtttcgtc ctctagcaga tggacaaaaa ggggataatc
    10801 gattaatgac cttaaaagaa atccaacaga tttatccgca aatacctgaa acaaaaacta
    10861 cttttccagg ttatggaggt attccaatca cacattacaa aaaatattgg tacattcaaa
    10921 cagatactgt taatacgtgt attgttggta cctctcgatc agggaaaggt cagattgaag
    10981 tgttagccac cattgacaat ttatctcgtg cagaaaaaca aagttcaatg gtcgttaatg
    11041 atccaaaaac agaattatat gttggtagta aagatgaact agaagctaga gggtatgatg
    11101 tatatgcctt taatatcacc gatccactgc aatcaatgag tgataatcca ttggcactca
    11161 tcgtgaaata ctggaaacga ggagacatag atactgcaac acagttaacc aatactttca
    11221 caaatacgat ttattatgat gccaacgctt cagataacaa atactttaac gaaaacgcgc
    11281 agaaagctgt caatgcgctt atttttgcgt tgttagaaca agctgatcaa acaggagatt
    11341 actccaaatt gacaccgaat aatctcgttg agcttctttc tgagattggc ggttttaatt
    11401 attatccaga tccagacaat gaattcaaac agttgaatgc cttagatgaa tttatgaatc
    11461 agttgccacc tggaaacatt gcaaaaaaac aatatggggc aacgaaaatt ggatctgata
    11521 aagcgaaggg aaatatttta tcaacggcaa tcacgggatt gaatccattt actctaacga
    11581 aaattgcaaa gatgacttct caatcaacga ttgattataa atcggttggg tttccaaaat
    11641 acttagattt gaaattacat gaaagtcttt taaatcaacg agtcgtagta gaattttatc
    11701 ataagggaaa gaaatttcat gaagaacgtg tcaaagtagg ctataaagga ttttgtgaaa
    11761 tcaattttga ttgtcattta gttgcaggtg atcaagtttg tttaaaatac gatcatcaag
    11821 gaaaagaata tgtggcttgg tatgaatttt ctcaaagagt attgaaagac gaagagggta
    11881 atatcgttta tcggaaaaaa cgaggagaaa cccatttgcc agaattagaa aaagaagccg
    11941 ttctaacatt agatgaggag aaaagtaact tattagtaga gtctattcgg atgcactata
    12001 ccgataaacc aatggctatt tttatgctta cacctgatta tgatccatct aaccatatca
    12061 ttgttagtat ttttcttagt cagttatata aagaattatc tacgcaatgt gttaagacta
    12121 aaggagataa atgtcaccgc aggattcatt ttattttaga tgaatttggg aatatgccac
    12181 caatggataa tatggaagga atcatgacgg tcacagctgg ccgaaacatg ttgtgggatc
    12241 tgtttattca atcttatcag caactttatg cgaagtatgg agaacaaaac gcagcgatta
    12301 tcaaagaaaa ttgtcagaca catatttata ttatgtcgac agatgataat accatcgaag
    12361 aattttctaa aaaagtgggg aataagacgg ttgaacaaga gaacagtacc atcaattcat
    12421 taggtatgaa tactcatatc aatcgcaatg ttgattcaga tcgtatctta actcctgaaa
    12481 gaatttctac ttttttagaa ggtgaaacga ttgtactcgc tcctttaaaa cgacaagatt
    12541 tgaaaggtca taaaatacgg ccgtatccta tttttaatca tggggaaacg aatcttcctt
    12601 atgcttatca atatattgaa gatttcaatc catcgtggga tatcaatgat atagacatac
    12661 attgtacaca tgcggacttg gatcttaagg ctttacaaat tgattatgat tatttcctac
    12721 cagatgaata tgctagaaat ctctatcatg gactgcatga tcaattagaa gaaaaaaacg
    12781 aagaaaatat gttttataaa atcgttcaat ctcttgagaa tgagacggct aaaaaattgt
    12841 taattcaatt ttacgaagaa caagcagcag atcgacttca agaatatgtt gaaaagttga
    12901 tcaaaaataa agaaatttct gaaatgattg ggaaacggtt gttattggaa tctgaaaagg
    12961 tggtgaaaga atgatcattg gaggaatttt ttctggcata gccgactggt ggtcaggctt
    13021 attcggagaa ggggatgcaa accccaaaac cgtaacgaaa gtcttacaag attatggtaa
    13081 ttatctgcag tatcaagatg tatttagttg gatttggaac acttttctgt ggggattgat
    13141 taaaggatta tataatctca atactttact agaaaaaacc atttatcaaa gctttgattt
    13201 aaaagactta ttgaacgccg ctggggtaaa tgcactattt caaagtttga ttagtaaggt
    13261 attggctctt ttttgtgtcg tcacattaat ttatttaggc ttgaaatttt ttatgagtaa
    13321 acacccacca aaagtcaaaa atatcttggt taatttattt atggccatga tcttgatttt
    13381 tggtgggagt tctttaatcg atgaagggtt aaatatcagt aaaagctttt acggagacgt
    13441 cactgcagca aataaagcag ataaaacggg ttcacctgct tttcaaatta ttcaaaataa
    13501 tgtgatcgat gtcagtacat tactagcaac tgacccgaat aaagtagaat ccctgccaac
    13561 agacaaaaga aatgctttaa cagcgaaaaa ttttaaaaca gcgaatatca atgggattat
    13621 tactcctaaa caagctaaag aaatggcgga taaagataag agtggtttat cagatttaca
    13681 aaaggatcgg ttaagagcgt tacaataccg attggaacta gatgataatg ggaaagaaat
    13741 tcctgttcaa attagtgacg aaggcttagc aaaatatgtc tatacttcag ggtatcgtcg
    13801 atacagtagt aaaccaggta ttatcttagc tggtgaatct tctttggcgg tggcctattt
    13861 atttatccta tttacgatcg ttacttgcat tattgagctg gtatttaaaa aattctattt
    13921 agtgattgcc gcgtcaacgg actttgagac aggccaacgg atgaaaagtg cgatagaaga
    13981 tatttctcaa tccttcttgt tattggcgtt tacgatttta gaattacgaa tctatacatt
    14041 aatgctttca ggtatcggtg atttgcatgc ggcaggaaag atcaatggat tcttgtatgt
    14101 tgttgcttta attgtgctaa ctattgcgct attcaaaggc tctcaatcgg ttacgaagat
    14161 tttcggtgtt gatacgagtc tgaaaaacgg cagtaattct cttcttagca tgtttgcctt
    14221 aggaaatatt gcgaaaaatg tgggtagcgg agctaaaaac cttgcaagtg ctggtaagag
    14281 tggtttaagc catttaaatg atattcgtaa aaatggattt ggccatcgaa aagatgttac
    14341 ccctgataac ggggaagaac aatcagacga taaagctaca actgtaccta atagtaagaa
    14401 aacagtttcc gaaatgttcg ggaaagcgaa aaatggtgtt tctaaagccg cagaaggtct
    14461 tggttacgtc aatgaacgtg ggttaaaagg aacagctgaa gatcttacag aaaaagccaa
    14521 agacacctta gaagagaaat tagacggcac gagagctaaa gaattggctg aagctgtgaa
    14581 gaatccatcc gaagcactag aaaaacaaaa agaaaaagct tcagaagtca aagaaggact
    14641 aaaagatgcg tatggtgaag gacaagttaa tgcagcagta aaagctaata caacaaccga
    14701 aaaagaaaat caagaaggta tagtgattcc ttcagtcaaa gatcaccaag aagcgttgca
    14761 ctctaatgaa aattcgattg aagagaaaaa ttccatagaa gaaagaaatt cagaagaagg
    14821 aaaaaatgga cagttaccaa aaactgatcc agaacaatat cgctataaac ctaacagtga
    14881 ccttgaaaat gagaagcgaa tttcagattt atcaaaagta aatcaagaag acaaagcaga
    14941 aaatgaggga cagttaccaa aaactgatcc agaacaatat cggtatagat ctgttaactc
    15001 cgaaaatgaa aaacgcattt cagatttacc aaaacgaaat caagaagtac aaacggatga
    15061 ggattccatc gttctgccaa atgataatgg aatcgaagtt ggaactgaaa aatcaacgaa
    15121 aattggttta cctaaggcac aaaatttacc aatgaaagat agtcgtttga atcaattcaa
    15181 ggagaatgat caaagctttg ttcaccaatc aactgcaggt actgttcaaa tgaaaccaag
    15241 tcgtttgcct gaaaatggaa atctgacaac tgtttcttac aatttatctg aaagtggtga
    15301 gacagggaat gcgtccgttc aaaatgttgt gacaagagaa gctgcaccaa aacctattca
    15361 aaacgttcat acaggttaca agccagcaaa tgtggcattt actgcaccaa caaaaataaa
    15421 tgtgacaaga gaaactaaaa acaggaacaa tgcattgaaa gaacaactat tgagggatat
    15481 caaacaggat atcaaaccat aaaaggccta caagttaggc cttttattta tatgaaaagg
    15541 agaaatcaat tatggataaa tatataaaca tgaaaaaccc tccaagattt agtggagaat
    15601 ggaagttttt cgcttttttc tttttagatc tattaacgct tggtggctgc tttttttggg
    15661 cgaaaatggt caataatctt gtccacttac cactcttttt tgcactcttg aattatggct
    15721 tatggattgc gatagcgtta gtgattgttt ggcgacccta taataaccca ggtcaacgac
    15781 aaatttttgc gattatcgag tctatttttt ttgcggacaa gaaccattat catgcgttag
    15841 atcctaaccg aaattatcat ggtacgtata aggaggatga ataaatgcga gtcaaaggac
    15901 aaaattcagt tgaaaggaaa tccattttgc agtttggaaa accagaagag cgctctatct
    15961 tggacttatt tccgtataaa tcctgtacag aagagggagt cttaattact agagaagatc
    16021 attttcaacg ttattatcgt gtagtttcta cagacgtgga aggattaaat gaacaagaga
    16081 aactagaacg aatgaatcag ttgaccaccg tcatgcgtac gtatgtgcca acaattaagt
    16141 tagttagttt gaccacagag acggatctaa cggaacaaat cgtgcaaaag cggcaattat
    16201 tagataaaaa tcgtatggaa cagtccttag gaagaaatct acaacgatta aaaaaatatg
    16261 aaaagaaatt agtcgaagaa atccaggaat tgaaacgagc agaacaagaa cgtcctgact
    16321 taagcttctt tttcgtcatt gaaggaaaga caatcaagga tttacaaacc agaaaccgtc
    16381 agttaatgcg atctagtggc attttagggt taaaaccact ctacaaaaaa gaattgatca
    16441 agattctgta tcgaatgaac aatatgaatg atgagtagga atggagaaga gagaatgaaa
    16501 tatccgttga tatggttaga aaaagatgtt ccgaaggaag agaccactag aaaatttcct
    16561 cacactttaa gcctgggtaa gttgggaaaa gggcaatctt ttaaccaagt aaagaatcaa
    16621 acaagaagag gtgactgtac atgttagaag caattaaaac agctttttca ccacaaaatg
    16681 atcaaaaagt acttgatcca ttaaagcaac taggaaatcg ggaaatagaa attttaaaat
    16741 ccaaagggta tgatttagat tttttaaaac aagtgatgcc acaaggtggg ttaaattttg
    16801 atgaagaata tgtgacgtat ggtaatggtt attcacgatg tgtgacagtt tatcactaca
    16861 caaaaaatcc tacgcttttt tggttggcca atctcatgaa caatcctcat acagtggcca
    16921 cactcgatgt tagcacagat gaaaagcaaa aagtgattga tcaaatcaat cgtgccttag
    16981 atgaattaaa tgatcgatca gataatgaac gaaaagcgac tgaccgaaat aaaagcgcag
    17041 aagaatacct ggaactagcg gaatatgctc gtaaattgac ttctaatggg gaaatttcta
    17101 agcaagtcaa agtgagagta catgtttacg gaaacacgat tcaagaagta gatgaacgag
    17161 cgcaggaact gatcgaaaat ctaaaaggga tggattatcg tgcggtgact tatctgcata
    17221 aaaccaaacg agattggcaa tcgatgttta ccatgttcga tgaacaagct gaatggttcg
    17281 gaaatatcaa agggcaatca ctaccatcgt taaacattgg cggaggtttc ccgttccacc
    17341 accaaacgct aaaagatcca ggagggacac aatatgggga aaccatgaca ggcggcccat
    17401 ttgtctggaa tgcgacgtat aaaaatagtg tacggttgtc tactaatacc ttgcttctag
    17461 gaatgatggg agctggtaaa tcgaccgttc ttaaaatgat cgcggaagcc catttagctg
    17521 caggagactt tatttggggc tttgaaaagg gaaaagactt tattcctttc ttaaaggaat
    17581 ataacggtat tatggtgcgt ttagacggat ctgacggaat gatcaatcct ttagagatat
    17641 ttgccacacg gacgtatgat gaagcatcca gtttatatga cgatggatca gtcaaagatt
    17701 tgaagatcaa tgaagcagcg tcctatcaaa cacacttaga caaagtggtc tatcaggtac
    17761 agttagtgag tcctcagcta aaaggaacga tgaaagcaga atttaagacc tacttaaatc
    17821 gtttttatga agagtatgga acagtaccac gcggctttac ttcctcaaat tcgcgttcaa
    17881 atacggagac gcaagtcaca ggtaaagatc cagaagctta tccgacgttc aaagaatttt
    17941 tagacttttt aggacaactg gaattaccag gtgcttccca agagaaaaag aatcgaaaag
    18001 aagaaatgga atccatcgta gaatccttat gtgagacgta tggcatgatt tttgatgggc
    18061 atagtacgat tcgacacctt gaccaacaac agttggtctt ttttgacatt gactctatta
    18121 caggcttaga tcgaggggta caacaatgtc aaatgtacat ggctatgacg ttgatttgga
    18181 atcaggcatt gattcaagga agaagacaac gacgtttaat taaagaaaaa caaatggaag
    18241 aacaagactt caaacgcttc ttggcgatga ttgatgagtg ccataatgcg attaatccag
    18301 attttattga aacggtaaat tatgtcacaa actttcaacg agaaatgcgt aaagtagaag
    18361 caatgactgt tttagctaca caatccccac aagaaatggt gccagaaaat atgtcttctg
    18421 ataacttctc gaaactgaag aaagtctttg aattttcttc tacgaagatc tttatgcgta
    18481 tggatgaatc tattttacgt catatagaag gattagtggg taaatcaatg acgaactcag
    18541 aattagaatc cattcctcaa caaaatcaag gagacgctgt gatcaatttt ggctcaaaag
    18601 aaaccattcg tgttcatttt agtccgaaca aacgtcaatt aaaattgttt gatggaggaa
    18661 aataatggaa aaccaaaatg aaagtttaat caaacagtat gttaagcgtc gtgcaaagcg
    18721 gcgcttattt ttgtgggtgt ttggtacttc tgcaggtttg attactatat tgatcactgt
    18781 gtttgtgacc ctttttctaa tacttgcggc aggttctatt gataattctg attcaagtag
    18841 tggtggcgaa gcttttacag gagaatattc ggaaggctta cccatttata aagaaatcaa
    18901 aggaagaggc cctttttctg atgagattgc gcaatatgct gtaggtgcag cagtgaagta
    18961 taaattatta ccaagtgtga tcctttcgca atacggttat gaatcagcgt ttggtacgtc
    19021 ggcgtccgca agaaatgatc tcaattattt tgggattacg tggtttgatg ggtgtctgtt
    19081 tcctaaagga actgcgcgtg gaattggtgg cattgaaggt ggctggtaca tgaagtttcc
    19141 caactcaaaa gctgccttct cctattacgg ctttatggta gccacacaat caaattttaa
    19201 tgcttgtgtc ggaaataaaa gccctggcgc aagcttattg atcttaggac gaggagggta
    19261 tgccgccgca ggaattactg aagacagtgc ctattataaa aattgtatgt ctattattaa
    19321 tacaaataaa ttgatggagt acgatgagtt tgcgattaaa cactggagag aaggcggcgg
    19381 aactggtggt acgattacag gaagctgggg caatccattc ccaggaagta gcttagataa
    19441 aaattcgttc agtggaggac aactttttgg gaaaaatcca ggaggagaat tccgacctaa
    19501 cgggttccat gatggactag actttggttc agtcgatcat ccaggaagtg agattcatgc
    19561 ggttcatggc ggaaaagtcg tgtacgttgg caatcctggt atttcaggtt tgggtgcgtg
    19621 tgtgatcgtg attaattacg atggcttgaa tatggtgtat caagaatttg caaatagtac
    19681 aggaaattct cgagtgaaag taggcgatca agtgaaagtt ggtcaagtaa ttgggatacg
    19741 agatacagca cacttgcatt taggatttac tcgaatggat tggcgtcaag cacaaggaca
    19801 tgcattcatt gatgatggta catggatcga tccgttacca tttttgaata gcagcaaaaa
    19861 atagatcagg agaaaaaata tggaaaataa aaccttaaaa atcgtcttaa cagctgcaat
    19921 tagcgttagt ttaatcggca atattgcgtt gttttggcag cttaaagaag aacaagagac
    19981 aaatacctat aaaattgaag aagtggctaa aaacgcgtct gataagctca aaaaaaattc
    20041 ttctgaggca caggcgaaga tcgacaagtt aaatcaaacc atcaatgaga aaaaccaaga
    20101 aatcgatcag ctgaagcaac aagatggcac aaaagcaaaa gaggaagtaa ctgaagcaca
    20161 aagaaagacg gcagaagagt tcggtcaatt ggccattgat aaatcattaa gcagaaatga
    20221 attaacggaa aaactaaaag atgtcgcaac ccaagaagtg atcgacaaat taagtcctgc
    20281 agacgatgat caccaacatg acgactacgg aagttactct tttacattag gtgacgtgca
    20341 aacttacgct caaacaagca gtgtaaaaga ggaacaaaat tttgtcgtat ttgttgatta
    20401 tacaatcggc aatcctcaat ttaaagatgt caaaccacaa aaaataaaag gtggattaac
    20461 tgtcactgaa aaacaagtag atggtcaatg gaaagtcaca gactttagtt atttcactcg
    20521 ttaggaggaa gaaaatgagt aagaaaaaag gagcaagaca acgttttaaa aaacgaaaaa
    20581 gttatcttag atggctgggg atctttatga ttctcggctt tttgattctt gctagtccga
    20641 tttacacaca taaaaaggta gacataccag atgaagtaag cacgaacgca tttttaaaca
    20701 cgaaggaact gacgatggtt tcaaaagaaa aaggagcgaa ccagcaatta gtttttgaat
    20761 ttgcgatcga cgatgaagat cagagcttat taaaagaact agctaatctt aattatcgtg
    20821 tagaagtaaa gacagctaaa ggggattatc aagcaataaa agccaaagtg atcaaggtgt
    20881 ctgatgatta ttttgtagtg aagttagcac atgtaccaga agaatatatc gccatgcgtt
    20941 taaccattat tcctgaaaaa attgatccaa aagtggatat gcaagaacca caagatctga
    21001 tttattacgt tcacgaagat aaggttaaag atcaagtgaa agatgaagat tatgaacaac
    21061 atgcgatcaa ttacaaagtg aaaggataca aaaaagaaca aaaagcagca caaaaacaaa
    21121 tggaatcctt ccaagcaacc attgatttga atgaagaatt agtgaagaag ctaaaagatc
    21181 aattgcctta tcaagtcgca gatgatcagg aaaatactga aaacaagatt aatagctacc
    21241 agcaagaaat tgaaacatcg aaaacgcaaa tgaaagatca agaagagatc attcaaaaac
    21301 ttcaagaaaa aattgatcgt atgacaaaag aaaagattta gaaggacagg tggagtgaaa
    21361 aaatgaagcg aacaaacttg gctgacaaag atactttcgc tgagcagaaa gtattgatta
    21421 aagcctatct caaagttgta aaagctaaag aaatatgtcg agaagtgaat ttatctaata
    21481 caacaatatc tgagttgaag aatggtcgaa agaatttaga tactttaaaa ttcagatcat
    21541 ttgaagctct atatgtagct gctaaaaaat attttacagc tgataacaaa taaaaaacag
    21601 gagtatttta tgtgaaaaaa gtatataaaa aatgcttatg aatcagctat atcaccttaa
    21661 ccatttgtta atgagatggg agaaaacatt aaccacgatt ctaacgcaaa catcaagcac
    21721 ataaaacatt taacactcct cctttctggc tgaagatttc aaccaaacag gaagacagcg
    21781 tatcaattat agcatacact ttttcttaaa ttcatttttc atatgacaaa atatttcctt
    21841 ctaatcgaca aagttagaag gattttttat tgtctaaata cacaaataac cttaattt
//
LOCUS       DANKHL010000029        21246 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000029 DANKHL010000000
VERSION     DANKHL010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.031"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(53..664)
                     /locus_tag="PW101_000754"
     CDS             complement(53..664)
                     /locus_tag="PW101_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2513178.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(787..912)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(994..1710)
                     /locus_tag="PW101_000755"
     CDS             complement(994..1710)
                     /locus_tag="PW101_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL2513179.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1716..2285)
                     /gene="scpB"
                     /locus_tag="PW101_000756"
     CDS             complement(1716..2285)
                     /gene="scpB"
                     /locus_tag="PW101_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDL2513180.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2269..3066)
                     /locus_tag="PW101_000757"
     CDS             complement(2269..3066)
                     /locus_tag="PW101_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDL2513181.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3036..3407)
                     /locus_tag="PW101_000758"
     CDS             complement(3036..3407)
                     /locus_tag="PW101_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL2513182.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3426..4313)
                     /gene="xerD"
                     /locus_tag="PW101_000759"
     CDS             complement(3426..4313)
                     /gene="xerD"
                     /locus_tag="PW101_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDL2513183.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(4409..4882)
                     /locus_tag="PW101_000760"
     CDS             complement(4409..4882)
                     /locus_tag="PW101_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2513184.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(4987..5850)
                     /locus_tag="PW101_000761"
     CDS             complement(4987..5850)
                     /locus_tag="PW101_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL2513185.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(6046..8145)
                     /locus_tag="PW101_000762"
     CDS             complement(6046..8145)
                     /locus_tag="PW101_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL2513186.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(8365..9474)
                     /gene="rpoD"
                     /locus_tag="PW101_000763"
     CDS             complement(8365..9474)
                     /gene="rpoD"
                     /locus_tag="PW101_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDL2513187.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(9498..11339)
                     /locus_tag="PW101_000764"
     CDS             complement(9498..11339)
                     /locus_tag="PW101_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL2513188.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(11521..13836)
                     /locus_tag="PW101_000765"
     CDS             complement(11521..13836)
                     /locus_tag="PW101_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2513189.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(14021..14881)
                     /locus_tag="PW101_000766"
     CDS             complement(14021..14881)
                     /locus_tag="PW101_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDL2513190.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(14940..15461)
                     /locus_tag="PW101_000767"
     CDS             complement(14940..15461)
                     /locus_tag="PW101_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HDL2513191.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            15739..16500
                     /locus_tag="PW101_000768"
     CDS             15739..16500
                     /locus_tag="PW101_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2513192.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            16526..19117
                     /gene="mprF"
                     /locus_tag="PW101_000769"
     CDS             16526..19117
                     /gene="mprF"
                     /locus_tag="PW101_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL2513193.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19305..20492)
                     /gene="tuf"
                     /locus_tag="PW101_000770"
     CDS             complement(19305..20492)
                     /gene="tuf"
                     /locus_tag="PW101_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL2513194.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            20860..21216
                     /locus_tag="PW101_000771"
     CDS             20860..21216
                     /locus_tag="PW101_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2513195.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         5818 a         4458 c         3721 g         7249 t
ORIGIN      
        1 tgtatttttt tccaaaaaaa aagaccaaga aatttcttga tctttttgat atttatttga
       61 ctgctgtatg acgttgttcg aactgatgtt gttttcgact taaccatgga agaagttgcg
      121 catgcagcac taaaaagaca gcactcacga tgaatccttt gatcaagttg aaaggaacga
      181 tcccaatcag tacatagttt gctaaagaca tgcccagcat ctgtgttgcg ttcagcccta
      241 aaaacattaa gtaaagaggc gtaataacga aataatttgc gatactcata aaaatagtta
      301 gagcgatggt tcctgtaatg acacctaaaa atttgtttct tcctttatgc tgtttcttta
      361 cactaaagaa ataataaatc gggaatgtaa agatcgttgt tgataagaaa ctagcagtgt
      421 ctcctaccaa attatccgga gaaaaacctg tagtcagcag atgtaatact gaacgcaaga
      481 acgctgttgc aactccggcc gctggaccga ataagaacat gctgatcaat accgagatat
      541 cactaaaatc gatcttcaaa aaactaaaag ccggcatgat cggaaaagca aaatactgta
      601 aaactaatgc gatcgcagca aacatcgcta ctgcaaccat tttctgtaca cgggtgttct
      661 tcatttgaaa tcctcctgtt aggactcctc ttcagcgaac tttttgacgt ttctctccaa
      721 atgaaaaaac tctattttcc agggaaaata gagttagagt tatttgatca agcgtcgtca
      781 aataagctct atcttcttta tccagactat actgtcggta tcggaatttc accgattcag
      841 ctattttttt gcaaaatagg tcgtggacta taaccaccgg tcgggaattt caccctgccc
      901 ctgaagacga accttatata atttttgttt acatattcat tatacatatc tattcaaaaa
      961 acgcaacatc taacacttat aaaaagtaag tgttcatttt gagtggttca gcaaactgct
     1021 gacttctttt ttgttcagtt cacggtaatc cccaggacgt aggccttgta aagtcagatc
     1081 tccgtatttt tcacgtttta gtttttgcac aggaagacca actgcttgaa gcatattttt
     1141 cacttgatga ttacgtcctt cgtggatcgt caattccacg atactgtggt ttgtcttagg
     1201 atccactgat aaaatttgga atttagctgg agatgttttt cgtccttcga tctttattcc
     1261 ttttgtcaaa ggtgcaagca tcgattttgt agcgatccct tttacctttg caacgtaaac
     1321 tttatccact tcatgttttg gatgtgtcag gcgctgagca aaatctccat catttgtcag
     1381 tagcaagatc cccgaagtat cgtaatccag tcgacctaca ggatagatac gttcttttac
     1441 catcggcaaa agatccgtaa caacttttct tcctttgtca tctgcaacag cagaaataac
     1501 tcctttaggt ttgtacaata aatagtaacc gtattcttct tgataaattg ggacttcatc
     1561 cacttccaca gtgtcatgtt tcccaacttt tatccctaat tcccttacaa cttgtccgtt
     1621 tacttttaca cgtcctgcaa ggatgaattc ttctgctttt cgacgagaag tgatgccagc
     1681 gtgggcaatt actttctgca atctctccat tacaattatt ctcctttatt cattccatca
     1741 aagaatagat ctggtgacac gtcttcttca agtgaaattt ccatttcttg tgtatcaggc
     1801 agttcttcta atgatttcaa accaaaataa tctaagaagt agccagttgt tccatataat
     1861 atcggccgac ctggtccttc tactctacct ttttcttcaa tcagtttatg ggcagctaga
     1921 cgctgtaccg ctcctgaaac ctgaactcct cgaatctgct cgatttcagc tcttgtgatt
     1981 ggctgtttat aagcgatgat tgctaaggtt tcgacggcgg cttgagataa tgtgtttgat
     2041 gtcccttgcg catatcgctt aagtaaggga gccagctctt ttttagtcgt taatacataa
     2101 tggctttccg tttcaaaaat atgcaaagct gagtgcgagt tttgagtgta agattctttt
     2161 aattctttca ctaaacttgt gacttctgct tcgctgagat ttaacagtcc gcttaattct
     2221 tcattactga tcccttcctc gccaacaata aaaagaaggg cttcaagttc acttaatttc
     2281 gtcatgttcg gtatctccta ctgctggata aagcataatt gattcatagt tttctttttg
     2341 ttgcgcaatg atctgccctt ttttcatcaa ttctagcaaa gccataaaag tagtgacaac
     2401 ttcactttta gaaggtgact caaaaaaaga atcgaaagta cagccatgcg catttctttt
     2461 tatacgctct tcgataaata ccattcgttc ttcgatcgtt gtgtcatcgg gcgtgatcgt
     2521 tttttccact ttttgagaaa atttcttacg ttctaagatt ttctgcatgg cacggatcaa
     2581 atcgattttt ttgacttgtc ctcgcttcaa cggcttttca gcatccgcta acccatcgat
     2641 gttcataggt tcttttgtgt aataaagact tcgttcctct gctttttctg tcagtctttc
     2701 agctgcatat ttatatttac ggtattctaa aagctgagtg accaattctt ctcgaggatc
     2761 ttcttcccat aattcttcat cgtctgtgac gaattcttgg acaggaagca atgtcttgct
     2821 tttgattgcc atcaatgtag cagccatcac taagtattct cctgcaacag ctagctcgag
     2881 tgttttcatt gtatggatat aattcatata ttgctccgtg atcgtagcga tcggtatatc
     2941 atatatatcg atttccattt gctgaatcag atgaagcagc agatcgagag gaccctcgaa
     3001 tacatctaat ttaatattga tctccgtcaa ctcattcact ctttcttctt tttctttgct
     3061 agccattttc caatgtaagg ctctgtgatt tcagttgcac ggatggagac atccgggtac
     3121 ttttcctgta attcatctaa catttgaaag cccatattct caccgcgata agacggattc
     3181 aaagaaatgt catggatgat cagctgatcg ttcgttgccc agtaaatacc aataatccct
     3241 tgaatgttgg ttgaaccttc tggcgtaaag aaaaaaagtt cataatcgtc ttcagattcg
     3301 tacgtatcaa tctccttcaa caaagaagtt ttacctgtca gtttcttatg gaaactcaat
     3361 agtcccatag caattttacg gttttctttc cggtaatgtg ttaacatctt ctcaccttcc
     3421 tatgactaag cccttggaaa atgctgctta taaacatccg tcattctttt cttcgtgata
     3481 tgtgtataaa tctgagtcgt agagatatcc gcgtgaccta acaattcctg tactgtccgc
     3541 aaatcagctc cattttctag cagatgtgtg gcaaagctat gccgcagcgt atgtggtgtt
     3601 acttctttgt aaatcccagc ctctcgtact aactgcttca gatttttcca gattccttgc
     3661 cgagaaagac ctttcccatg attattgaca aatacatgca tttcagatgc gtcctttaca
     3721 agtaatggac gtgcttcgtc taagtagcgt tccagccatt ggatcgcata atcacctaaa
     3781 ggaatgatcc gctctttatc tccttttcca actgtctgta ccagtccaag agaaagatga
     3841 agatctccca atttcaatcc aatcaattca ctgacgcgca tcccagtagc atacatgact
     3901 tccaagattg cacgatctct tatccctaac gtttttgtcg tatcaggtgc ttcaatcagc
     3961 ttttctactt ctgtcagagt caatgtactt ggtaatttct gtgctttctt cggcgtatcg
     4021 atatgttgca tcggatcatg atcagtgata cgctcttgcc gtaaaaactg atggaatctt
     4081 ctgagacttg agatcatccg agtaacagtt gctgtcgctt ttttttcttc atgcaacttc
     4141 tccagaaaac ggatgactaa aaagcgatcg acatcctgcc atgaagcaat tttctgctcc
     4201 tgtagaaaat gcaagtactg ttccaaatca cgttcatagc tttgtcgtgt attggcggac
     4261 aatccccgtt caatattcaa gtagtggaga taatcgttga tttgctcttt cataataatc
     4321 cccctcatgc ctctctatca tatcagatac cgcttcattc tgatagtctt tttccataat
     4381 ctgagcaaag aaaaagccgt ctagacactc agacggactg gtttcttttt tctttttcat
     4441 agcattcctg acagattcca tggaacgtta gccgatgatc ttttactaaa aaatgataac
     4501 gttcttctac tacttgctcc acttcaagta aaagatcctc ttctacttcc tcgatcgtgc
     4561 cgcagttcaa gcatagtaaa tgatgatgga aatgtttggc gccttctttt ctcaaatcat
     4621 aacgggctaa tccatcatta aaacttactt tatcgacaat tttcaattct gtcaagattt
     4681 caagggttcg ataaacagta gcaagtccaa tttccggact cttttttttc actaaaaaga
     4741 agatttcttc tgcagataaa tgctctttct cattttccaa aagcacaagc agagtagctt
     4801 ctctttgcgg tgtcagctta aaccccgctt catgtagctg ctgttttgtt tttttcatcg
     4861 ccgaaatagc attcatgacc atttactcaa ccccaacaat ttataataat tacaaataaa
     4921 aaaagatata acactatgtt aattaaaatt attataagct agttaagaaa aacatgcaag
     4981 ggattctcaa ttagattttt tgactagttt tgtcacaccg ttttcttgtg taatcaatcc
     5041 ttgtttcaaa agattaccta atgcgcgctt aaatgctcct ttgctgatac caaaagcttt
     5101 catgatttct tctggatccg atttatctgt aaagttgatt tgctgatctg cagaacgttc
     5161 taaaatggtt aaaatcattt gtgcatcatc tgatatagct tcatatgctc ttggcttcaa
     5221 tgaaagattc agtacaccgt ctggtcggac accaatcact cgacctttta tctgctcgcc
     5281 tagacgaggt tcattatatc gttcagaagg atggataaac ccaagataga aatcttccgt
     5341 taaaacgtac gtacctgtta atttcaagcg gtatgctgtt cctatcacat ctttgttttt
     5401 catttcttct gttccatatt tgcttaaaga tttgaatatt ttttcatctg ctaaactgcc
     5461 ccaaatacgg tcttttgcat caactctaag ggaaatcatc aagcgatccc cttttttagg
     5521 ccatagctct gtcattgtcg gcaattcatc aagagatacg acaaagtctt tatctggaag
     5581 accgatatcg acaaacgcac ctaaatcttt tcgtgtagca gtaacgattc caaaagcgta
     5641 atgaccgata cggcttttag gaatttgtgt agtgatccga ttttcatgtt tttgattttg
     5701 gtaaccgaat ccttcaactg cttcaccgat tttgtgttca ccttctgatt tgcttaagcc
     5761 aaaggtttgt ccgttttttt ggacaaagta gtttttttca ttttcatcaa taataatacc
     5821 tgtaaatata gttgctaata aatcgttcat ttttttctcc ctctatagtg aaaggttttc
     5881 ttaatacatt tctttgctat cataacataa tgttatctac ttgaatagct gagccttggt
     5941 ttggaaaggt gaaaaaagga gtatgacaac agttgtgtca tactcccctt tttcgaataa
     6001 acggtgttaa acggcaggct cacagcctct ttcatttgcc taaaattaat tgctcccatt
     6061 tcctccgaac catccagttt tacagttgat ggttacgatg taaaatggca aagcactttc
     6121 tccaatgcct ttcatcaatg cagattttgt tgaagtggca tatagattat aatagccttc
     6181 cccattttcg attctggctt tttctaatct gtaacctcct tcaacgatat atccacgttg
     6241 gatacatgta gcaatcactt tgctttggac acctggtgcc cacgtccatg ctggattaga
     6301 tgcatcggca atatcacttg ctctggcaga tttgattggc tgattcgctg agtttgaaga
     6361 ggaaggggtc gaggatgccg cttcttgctg tgctgcttgt gcgaccgcag cttgttgagc
     6421 ggcttgagct gcagctgctg cttgagcggc ttgttttgct tgttcttcag ctgctttttt
     6481 cgcttcttct gcttttttct tttcctcttc ttcttttttc tctttatcag tgagcatctt
     6541 atctacttgt ttcaattgtt cattcaactg agcagataga tctgtatttt tcactttttc
     6601 tactgcttct tttgccgtct tgtattggtc gcgtgttgct gaatccacta cttttccgtc
     6661 tttaaagact atagctgttt tttctttagc tgtttgcaat tcagcatatt gatttttagc
     6721 ttctgtttgt gcttgttgaa tcaattttcc aaaatctgtg ttatccgttt cgattgcttg
     6781 gatctcttta tctgctttca acttgatgtc atctgctaga tgatcatcga cgatggcagg
     6841 tgctatgaac cactcattca ctttttggat atctcggatc ttagattgga tatcttggta
     6901 agttgtttct aactctgaat atcctttttc attcttaaga ctggataaat cattttttag
     6961 ttttgataaa tcttgatgaa tcatatctgc acgaataaat tgatggtgat catctgtata
     7021 aaaggcagca agtttctcat gaagttcttc catttgttgc tgtctttttg cttctgctgc
     7081 tacttcggct tctacttttt gatgatggtc ataataagcc cagcttcctc caccgagtag
     7141 aagtactgct gctaaaacga gataaggtac tttttttgat tttttctttt gcgctttatc
     7201 tggatctgtt ttttctaagt ttgattcttg atgtgttttt tcagtatctg gcacgattgt
     7261 tattgatttc gcttctgctt caaacagctt aggctggttc tcttcttttt ctatttttgc
     7321 ttcaagatca gtaccctctt tgcttacaga ttgattttca gcatcggatt cactatttat
     7381 ttcatcgggc tctgttgact ttttgtctgt gttttcgagt atttcttttt tctctgcttc
     7441 cggcagttta ttcgtttttt ccttttctaa ttgtttttca gtaaatactt cagttttttc
     7501 tgtgatatcc gtttttacgt cttttatttt tactgtttct ttacctgtgg cgttttctct
     7561 atcagagtct ttttcagttt cttgttcctt ttctggctct actttgtttt cctgtgcaat
     7621 tttttcactg atcattttga gcagctcttt cgcgtgctct tcttttgtat cttcacgatg
     7681 tgctttgata taagcggata gtatcgaatt ttcttcctca ttatcgacga tttctttcag
     7741 ttcagctact cttctttttt gttttgattc aagcgtttct tcttgattct ttcctgattc
     7801 ttccgaagtc tcatgaacag ttgctttctt ctgttcattt ttctctatat tagttacttg
     7861 atcgtgagca ggtggttctt cgtcttctgg ttctgctttt tcgaataatt ccattacaga
     7921 ttctagaggt aaatctttca attccgacca ttcgattgga tcgtttaatt ctgtatcagg
     7981 aaagactgca tcttcagttg atttatcctg catagctgca tctgtatttg tttcttcaat
     8041 tgattctttt atttgctgat cttctgcagt tatttgatga ccgcagtctg gacagatagt
     8101 attctgctcc atttttttcg atccgcaatt tggacatttt ttcatcactt agcgatcggc
     8161 tcctttacta ggtttacgct accatttaat ctatcatatt ctatacgtca cgccaatagc
     8221 ttatttttag gatttactta aaaataatga atgaatttcc ttcctataga aaaaaaagct
     8281 tgagactgcg tagtctcaag ctttttttct tttaaaataa accaaacagt tttactgatt
     8341 tctcttctgg tcaatagtcc acgcttattc taagaaatct tttaattgtt ttgaacgaga
     8401 aggatggcgc aattttctta atgctttagc ctcgatctga cgaatacgtt cccgagtaac
     8461 accaaatact ttccctactt cttccaatgt tctcgtccgg ccatcatcta aaccaaaacg
     8521 taagcgaagg acattctctt cccgatcagt caatgtatct agtacatctt ccaattgttc
     8581 ttttagtaac tcataagctg cgtgttctgc tggacttgtt gcttcctggt cttctatgaa
     8641 atcacctaag tgagagtcat cttcttcacc gattggcgtt tcaagggaaa caggttcttg
     8701 cgcgattttc aagatttctc ggactttttc tgttggaaga tccatttccg caccgatttc
     8761 ttctggcgta ggttctcttc caaggtcttg cagcaattga cgttggatac gtatcaattt
     8821 attaattgtt tcaaccatat gaactggaat acggatcgta cgtgcttggt cagcgattgc
     8881 tcgagtgatg gcttgacgaa tccaccatgt agcataggtt gaaaacttaa atcctttgcg
     8941 gtagtcaaat ttttcaactg ccttcatcag tcccatgttt ccttcttgga tcaagtcaag
     9001 gaactgcatg cctcgtccta cataacgttt agcgatactt actactaaac gaaggtttgc
     9061 ttctgccaga cgctgtttcg cttcttggtc gccttcttcg attttcaatg ctaacgctac
     9121 ttcttcttcc gcagttaaaa gggagacacg gccaatttct ttcagataca tacgaaccgg
     9181 gtcattgatt tttacaccgg ttggcgcaga aagatcttct gtttttgctt gttcagcttt
     9241 tttctcagca ctcttcaagc tgtgaattga tggatcacca ttttcatcca ccacactgat
     9301 acctgcgtct tcaatctttt ggatcaattt gtccatttca tctgcattca aagtgaatgg
     9361 tgtagcaagc tgattggata aatcatcata tacaacttgt cgtttgggtt tgttttcttt
     9421 gatgaaagct gctactgctg cttcatacgt ttttttatct gttccatttg ccataaaaga
     9481 aggcccccct tgagtagcta tttggcttgt tttaactgtt tcgttaaatt gatgatttca
     9541 atcgtcaatt caagctcaag ctgtttgttt ccagttcgtc tggcttcttg ctgcatttgt
     9601 ttttttaatg ttatttcatc agctaagcca gatttgctga tcacatctag caagtcttgg
     9661 atttctcgtg gcgaactttc ttctgccacc ggtaaatatt cgatttccac caccaaccgc
     9721 cttaaatttt catcttgcaa aaagtctaaa aactctgcaa ggttgaattc ataatgttgt
     9781 gagacatatg catcgaataa aaaataaact tctgcatatg catcatgaat aaactgaaat
     9841 tctcgttctt ttaaaatatt acggacagaa acttctttaa atgcacgata taatagaatc
     9901 tgctctgcct tttctacttg cgtcaaaggt ctctttttcc gtgcagtttc ttgtctcgta
     9961 acctgagtgt gttgactttc ctgaggttct tgacgatttt gctgatttgt ccttcgaaga
    10021 tcgcgcagtt gtttttgtat cgtctctctg ccgatctgaa actctgttga cagttgagat
    10081 agatacatat cttgttcgat tggtgaaaca actcggctaa gttcaactag taattcttcg
    10141 aggtattcga tcttgtcttt ttcattatct aaatttttcc cttgttttaa ataacgtgat
    10201 ttaaaagtaa agactgtttc gcgcccatga gccactaatt cttggaaaga tgcttctcca
    10261 tacttacgga gatattcatc aggatcaagc ttttctggaa cgactacgac agaaagtgct
    10321 aatcgactgt tttgccgaag caattcaatc cctcgattgg ttgcttcaaa acctgcgttg
    10381 tctccgtcat agcaaaagac caattctttc gtcagctttt ccatttgttg gatctgttga
    10441 gctgttagac ttgtccccat agaagctaat ccagttttga taccagcctg ccatgcagca
    10501 atcacatcca taaacccttc gaacaacaaa atttctccgt ttttccggat atcgcttctt
    10561 gctttatcaa gattaaataa aacttgtctt ttattgaaca gttctgtttc tggactattc
    10621 aagtatttgg gctgatcttt ttcctgccct ttttcaggtg caagccagcg tcctgaaaaa
    10681 ccgatcgtct ggccttgaga attacgaagt ggaaacatca ctctttgata aaaacgatca
    10741 gacatacggc catcatcatg cgtgacaaac aatccagagc gttctaacaa ttcatttgaa
    10801 attgattcat tcttgaatac ttgctgaaga aaagaacgct cattaggtgc aaaacctatg
    10861 ttgaattctt caattaattc taaagacaac ccacgacttt gcaaatattc taaagctggc
    10921 tgtccagctt ttgtatgcaa tagcatgtga tgatagattt ccgctgcttt ttcatgagca
    10981 gcaatcagtt tccccgtagc agattcagga ttttggacag ctatagtacg ctgacgccat
    11041 tgatcttcta acggtatctg ttcgaagtct gccactttta ccactgcttc tggaaaactt
    11101 agaccttcta gttcttgaag aaaagtaaac acgtttccgc ctttaccaca accaaagcaa
    11161 tgaaaaatct gcttgtcttc tgcaacagaa aaagacggtg tacgttcttc atgaaaagga
    11221 cacagcccca agtaattttt accagacttt ttcaactgga catattgtcc aatcacctca
    11281 acgatattcg tttgactgcg tactgtttcg ataacctctt gaggaatccg ttgagccatt
    11341 ttttcacccc ctgctctatt gatatctttt gggtttaaca gaagatattt caggagaata
    11401 accggtaaaa aatcgcacta actgaaatca gtgcgatttt aaaggacaca attatacatg
    11461 tttgattgta tcatatttca ttgctttttc aagtataaca catttctatc tcattaagca
    11521 tcaatttgct actcttttca gtaactgatt ttgaatatca gccttccagt acaatactcg
    11581 aagaacaata tttacgagca tgaatacaaa gaagtatcct acgctctctc ccaaccctaa
    11641 gtttacttcc aaaattccat ggaacagcat tgcagctaca tatatcccta cgatcgtcgt
    11701 tactgctagg aagatgcgca atgggttgaa gggataacaa gcttttacaa ctgccatgca
    11761 actgatccca atcagcaagt aatacatcaa agtagtagtt tcagcatttc cccatgcgaa
    11821 tgtttttcca agtagataca ccgcaatgat attcactact acaagcaagg catttggtaa
    11881 agcattaata agtgctgtag gcaaataacg gtaagtgatt tttcttttat cttgttcaaa
    11941 agataaaaag aaggaaggat acccttcaat cgctaaatca atcagcgtga tttggatagg
    12001 gataaacgga aatccaattg ctgtaacggc acatataatc gataaaataa atgaataaat
    12061 cgttttgata aaaaagatgc ctgagacttt cgtcacattg ttgaccactc ggcgtccttc
    12121 aaacaggact tctggcaatg tagtaaaatc agagtccaac aataccagat tcgcaatctg
    12181 acgtgttgct ccatcacctt ctgccatagc aatgctgcaa tctgcttctc ttaacgccaa
    12241 aacatcattc acaccgtcgc ctgtcattgc aaccgttctg ccgctttctt tcaattcatt
    12301 taccagcaat ttcttttgct gcggactcac tcgtccaaaa accgtatagc gatgagcagc
    12361 ttctcgcact tccatttctt cagtaatttg agaaagatcg acatacgcat cataattcgg
    12421 taatcctgca cgtctagcga tattggagac agtaaaagga ttgtctccag aaatcacttt
    12481 cagatccaca ccttcctcat gaagataagc tagtgtttct tgagcgtttt ttcggattgg
    12541 atcatcgatt tcaaacagag caaccggctc aacaaatgct ggtttttctt cgttcaattg
    12601 ctgatttgga gcgatcccca acataagtac gcgatagcca ttcgcttggg ctgtttttag
    12661 actgtccgga agccgatctt cttccaccaa acgttcaggc gctcctacgt acacgactcc
    12721 cagttctgga aattccatcg cgccccattt gcgatcagaa gaaaatgcga ctttgtttgt
    12781 cacatcatac cgattagaaa gctcaaaata atctctcata gcccgcatag tgatgttgtt
    12841 atcactgctt tctgccagat acgtccccat aatttctggg atggctttct cgtacagcgg
    12901 atgtagtgct tctacccgct ggatactcat tttcccttca gtaatggtcc ctgttttatc
    12961 caagcataat gtatcgacat gggccagcgt ttcaattgaa tacatgtctt gaacaaggat
    13021 tcgttttttt gccagtttgg ttacccctgt cgttaatgca atacttatta gcaataccaa
    13081 accctttgga agcatgccta acaaagctgc tgcagatgca ataacagaaa ttttgataga
    13141 agcatcgcgg atgaacagtg cttccaaaaa tagtatgatc cccaaaggaa taatcacgaa
    13201 actagtgaat ttggatactt tgcgaatcga atctacaagt tcagaacgaa tagctttgtg
    13261 agtctttgcc tcgctagtca agcggacagc ataattatct gcacctactc ggataacttc
    13321 agccatgaca gaaccgcttg tcaaaaagct tccagaaagc aattcagctc cagtttcttt
    13381 gacaatcaaa tcagattcgc ctgtcaaaag agcttcattt gcttctgccc gtccagatat
    13441 aacctgtaaa tcggctggta tctgttctcc tgctccgatt tttaccaaat ctcctaatac
    13501 cagctcttta gtagaaatcg attgttcttt accgtcccgg atcactagta cttctgattg
    13561 ggaaacaatc gataatttcc gtacaagatt tcttgcatgt atttcttgat aaattccaat
    13621 aacgatattg acgacaataa ttgctaaata cgccatgttg gaatacgcac ctacaaataa
    13681 cagacaaatc ccaataccaa agttcagaaa attgaataac gtcaatatat tatcgctgaa
    13741 tatttgtttt gtactttttg atgcatcttc ctggtagtca ttttgctggc cagaagccat
    13801 tttctgaact acctcttgct gtgtaagtcc attcattttc tttttctcca ttttccgcca
    13861 actttcaaaa ataatcacgt gtttaagtct agcgtgttcc aagattaaaa acaacttgtt
    13921 tttataaaat ttatagaaaa acacacgaat gattttacaa aaaagatgcc aacctttttg
    13981 tttgaatcac ccgtgtgctg tttttattat caatctatct ttacttatat tgttcctcca
    14041 aacggctcat ccaccaacct agtgctgtac cggctataaa aagaacagga caagctagta
    14101 ccccagcaac tcccaatccc gcgtaatcgg taggaatgat cataatcgtt gaagcaaaaa
    14161 ctacacctaa aatgaaatgg aatagcttgc tatatgcctt tgagaaaatg taatccatga
    14221 tttttgacaa tccaagaaca caaagaatcc caccaattcc gatcggcaca atcacttcaa
    14281 gtcgtacttc tttaaatccg tcagccatcg ctttatacaa tcccatatac accaaaaaat
    14341 tagaagggct caatcctggg acgatcatac ccaaaccaat caatccgccg gcaatcagcc
    14401 atgttccaaa attctgatcg acttgcccgt caaataattg agcgccgtat aataaaaaca
    14461 aataagccag aacaaaagta acaaccatga tgatcacatc agaagtatct cttccttttt
    14521 tccctgcttc tttccataac gcaggaacag ttccagcgat acatccgaca aaaaaccata
    14581 atatcgttgt ttcatacgca cctaataaaa aactgactaa aaaagaaagt agaaagatgc
    14641 ctgtcactcc gcctagacca acaggcagaa aaaacaaaac attttctttg aaattttttg
    14701 taaggtgggc taaaaaagaa atcagccgct catacatgcc aaagacagct gctaatgcac
    14761 caccgctgac tcctggcaag ataaatcctg aaccgataaa cattcccttc acaaaccgta
    14821 acatccagtc tttggttgtg taccccactt cttgattttc cattttttgc cttttttcca
    14881 ctatttgcgt actccttctt tcatttaatt ttattcatga ttgaacattt cctgctattt
    14941 tacttaaaga ctttttcata ggtctctatt attttttcat tcattttgac aatatcagga
    15001 taaaacatgt tacctccatt atgatagaga tagccgccat tgtaaagcaa ggactggatc
    15061 cgccaatatc ggtattgttt cccttcctca tttcctaaaa gtgggctcag cacatcttta
    15121 gaatacgctt ctgctaattc tactgtgttt tctccgccat gattttttac ataggcaata
    15181 taatctttcc caaaattata tgcttggaca gctgtccata aatcgcatcc ttgtttcttt
    15241 gcttctacta tcatttccgc caaatatttg acaccttgat caatactgtc agctggatta
    15301 tcaatgatct cagcttctcc atgcagactt tccgagctct gcatgagatc tgtccctttt
    15361 ccttttgatt cggtcaatat tatcgccttg atcaaattct cgtagttttc catatgataa
    15421 ttttttgcag ctgttgttat ttctatttga tagctttcca ctcgttttac cgaacgataa
    15481 gttcttcctc ataaaaacaa caccgcacca ataaccgtca gaatgatcat gtaaaaaaga
    15541 atacggataa tagataatcg ttttcgcttc atttcatcac ctagttattt tgacatcttt
    15601 ttttatctta ctatttcttt atcttcttgg ctatttcttc cttgatttct gtgtacgcct
    15661 taagaaaaaa tgaattttcc tgtatttttt gacatcttac gaaaatgatc gttgtttttc
    15721 agtaaaaaca attgcccaat gactgatata atcgttatac tttacacagt aggtgattat
    15781 gtgaaaaaag aaaagaaata tgcaaaaatg ccagatggga gcgaaattta ctatgaaaag
    15841 agcggtcaag gcttccctct ctttttactg catggcaatg acggcagcgg ccgctttttt
    15901 tcagaacagg tccctgtact cgaacgctac tataccgtgt atctagtaga tagccgagga
    15961 catggtcgtt cgacaaacga agcatctatg ttgaattttc agttaatggc cgaggatctg
    16021 aatacgatca tgttgctgga aaaaatcgat caggcagatt ttcttggatt tagtgatgga
    16081 gcaaatctag cactggtatt tgccagcagc ttccctaaaa aagttcatcg attgatttta
    16141 aattctggaa acacgcttgt taaaggcgtt cgtttttctg ccagagtcat cagtaatatt
    16201 cattatgcat gggtatggtt actctcttta tttcgcccaa gtcttcgcaa aaatctttta
    16261 gttatcaaac ttttgctgca tgacatcggg cttacagaaa atgatttaaa aaaaatcaac
    16321 tcccccactt tgatcatcgt gggaaaaaaa gatgtaatca agctaaaaca ttctttgtat
    16381 atagctaaaa ctataccaaa agcttccttt gtgcttgtga aagaacaagg acatgaatta
    16441 gctagaaaag atcctgaacg cttcaatcga gaagttcttc aatttctttc tgaaacataa
    16501 ctattaggaa tagaaggtga tttatttgtt aaaaaatacc atacaatggc tgaaaaacca
    16561 cctaggactg ttcaaaacaa tctttttgat ttctgtcatt gtcattattg tcggtgaatt
    16621 gatgtccatc ggtaaaactc tatctatcca gcaattagga gaaacgtttg ccactatacc
    16681 tgtatggaaa agcggactaa tgctattgat tggtcttatc tctgtgttac ctatggttgg
    16741 ttatgatatt attttgaatc ggatgcttga acaaaagcag aatcctcgtt acctgtttga
    16801 gacaagctgg ctgatcaata cgatcaacaa tattgccggt ttcggcgggt ttgttagtgt
    16861 ggggcttcgc tctgaactat atggacaaaa gaaagagagc agaaacgtga tccaagcatt
    16921 atcgaaagtt ttcttgtttt tgatggctgg actttctatt tacagtctgc tttctttttt
    16981 acttgtagtc ttcacgccag ttgctccttt tttgaagcaa tattggatat ggttgatcgg
    17041 cggcggtcta tattttcctg tcgtcttttt atttactact ttcaaaaaga aaggattcct
    17101 tggtggtacc tcacttaaaa ctcaaggaca gctattgcta gtttcattgt tggaatggac
    17161 cggtgtcctg tttagtttcc tttctgttgg ttatttaatg gaaatccgga ttgatctgtg
    17221 gcaaacaatt ccattattca ttgctgcttc agtgatagga atcgtgtcta tgattccagg
    17281 agaaattggg agtttcgatg taatgatgat tatcgggttg tccgctattg gtgtgccaag
    17341 agaaatagtc gttgtgtgga tcttgctcta tcgcctgttc tactacatca ttccgttttt
    17401 gattggaatc gtctttttct tcaaaaatat cggttccact tttgatcagc gttattctgg
    17461 tatccctaaa caattagcaa cagaaattgc gcataaaatc gtggttgtct tattatactt
    17521 ttctggaatc atgctagtac tttctgccac tatcccgcaa gcttttacag agtttcgctg
    17581 gcttcatagt ttgaacccat tgaaatttca ttttattatc caatttccta gtatactatt
    17641 gggatttctt ttaatcatta tgggcagagg gatcgcagca cgtgtgaaac gagcatattt
    17701 gcctacaatc ttcttgattg ttctagcttt actttatgtt ttattaagtg attttagttt
    17761 cacaccggtg gttttcttgt ctattctgct tctgatcatt cttgcttcta aaaacgaatt
    17821 atttcgggaa caattgatct attcatggga gtggcgtacg atcgatggaa tcattattgg
    17881 tgcactgtcc ttgctttaca tcgttattgg ggtatacaat ctgccggatt tccctcatcg
    17941 gcgtcaccat tttatttcct ttttcttatt tccttctgaa aagatttggt tttcgggatt
    18001 gttggccatc atcgctgtta gtttcatgat cgttctattt gttcatttcc ttcaaggaga
    18061 aaagaaacag attggcgaag cctttaatga agaaaaggcc ctgaagatcc ttacaactta
    18121 tggaggaaac tccgatagtc aattgatctt cttaaaagat aagcggatgt ttgcctatga
    18181 aaaagacggt gaaccaactg ttcttctcca attcgcttgc ttcaataata aatgtattgt
    18241 aatgggtgat ccttctggta agaaagaaga ttttccagaa gcaatcgaag cttttatcga
    18301 agaaacagat cgactatgct atttaccagt tttctacgaa acaagcgaag agattgttat
    18361 gattttgcat gaattcggct acgatttcat taagatgggg gaagaagctt atgtagacct
    18421 gaatagtttt acaacttcag ggaaaaaaat gaagggcact cgtgctgtct tgaatcggat
    18481 cgaacgagaa ggatttacat ttgatgtctt acaaccacca ttttctactg agcaaatgag
    18541 tactttcaaa aatatctctg ataattggtt gggttcacga aaggaaaaag ggttttcttt
    18601 aggctttttc tctgaagatt atctacaaag agcgcctatt gcagttgtca aaaataccca
    18661 agaagaaatt gtcgcttttg caacgatcat gcctacttac acgaacaata aagtaggaac
    18721 gattgatctg atgcgatatg attctaaaaa agcaccttct ggcagtatgg atttcttgtt
    18781 cttgcatttg tttgcctata tgaaagaaga acatatccaa tggttcaatt tagggatggc
    18841 ccctctttca aatgtcggaa cttccagaaa gagtttttta caggaaagga ttgcatactt
    18901 ggtttacgag tttggttctc atttttattc tttccatgga ttaagagaat ataaaagcaa
    18961 gtacgctaca aactgggtct cacgttatac tctatattct agagatagtt ggattgcata
    19021 tgttatgatt gctttgctga ttattgataa tgcgccagtt gagcagacac aaggtagaat
    19081 aagtggtttg aaaaaatgga tacgaagaaa gtattaaaaa ctgcataccg atagatgaac
    19141 actctcatga tgaataaaag agactgaagt aaaacgaaag ttcatgcttc tacaatctct
    19201 tcttatacaa ttgaagaaca aagagcggtc caaacagaaa tcctgtttgg accgctcttt
    19261 tattttaaac atttttcatt accaaaatat tcgagtcgcg tttcttatgc ttcgatctct
    19321 gtaacgatac ctgaacctac tgtccgtccg ccttcacgaa ttgagaatgt cgttccgttt
    19381 tcaacagcga ttggatggat caagtcaaca tcaatcgtca cgttgtcacc aggcatgacc
    19441 atttctgtat cttctggcaa tgtgattgtt cctgtaacat cagttgtgcg gaagtagaac
    19501 tgtggacgat agttgttgaa gaatggagta tgacgtccac cttcttcttt tgtcaacacg
    19561 tacacttctg ctttgaattt tgtatgtgga gtaatggaac ctggtttagc aagtacttgt
    19621 ccacgttcga tatcgtcacg ttggatcccc cgtaacaaca cgcctacgtt atccccagct
    19681 tccccgtaat ctaacgtttt acggaacatt tctacacctg ttacaactgc tttttgtgtt
    19741 tcaggtttga tccctacgat ctctacctca tcaccgacac gaacagcacc acgatcaata
    19801 cgacctgagg caacagttcc tcgacctgta attgagaaga cgtcttccac tggcaatagt
    19861 aatggtttat ctgtatcacg ttctggtgtt gggatatatt catctactgt atccatcaat
    19921 tccataatag cagcttcagc atctggatcg ccttgcaatg cttttaatgc ggaacctttg
    19981 atcacaggag tgtcatcgcc tgggaaacca tattcgctca ataactcgcg aacttccatt
    20041 tctaccaaat cgatcaattc ttcatcatcg actaaatcaa ctttgttcaa gaaaacaatc
    20101 aggtatttta caccaacttg gcgtgacaat aaaatgtgtt cacgtgtttg aggcatcgga
    20161 ccgtcagttg cagaaacaac taagattgca ccatccattt gtgctgcccc tgtaatcatg
    20221 tttttcacat agtccgcgtg tcctggtgca tcaatatgcg cgtagtggcg tttttctgtt
    20281 tcatattcta cgtgtgcggt attgatcgta atcccacgtt cacgttcttc tggagctgca
    20341 tcaatactag catagtcttg aggatttgct aggccttttt tgcctaatac tgtagtgatc
    20401 gcagcagtta aagtagtttt cccatggtca acgtgtccaa tcgtaccaat gttaacgtgt
    20461 ggtttacttc tgtcataatg ttcttttgcc ataatttaca aacctcctaa ataatataga
    20521 tcactgaaaa atttcagctc tcaccttttt attatagtct ttttttgact aaatcaaaaa
    20581 gaatacgtcc gacttacttt ttgtaagatt cgaactaagc ttattatacg tcaatattgg
    20641 tcaaagtcaa ctaagagcac tcgtctgtct tatacgcgaa aaaagtacct tttataaata
    20701 aaaaaatgtg ttttttgaga aaaacttcaa gatagtcctt aactttcttt tgaagcaata
    20761 agaacaaaat gaggaaaagt ttaaaattca gctgttgcca acaactttca atagacgaaa
    20821 tcttctattt cgttaataat aaggaaagag gtgaagtaca tgaaaaaatt aattggcgta
    20881 cttgtcgttt taggcatatt tctcggcgga agttttgcag cttatcatta tttttacggc
    20941 ggcgaagctt attatacaaa aatcacgacg aatggcgaaa tgtcagctac ccaagcagat
    21001 aacggagaga aattcacgac ttatacctac aagcaagcgg cttataataa agatggtgaa
    21061 aaaaaacagg tgacgcttcg cgaagaaaga gaacatccat taaagatgaa tgcgtatctt
    21121 aaactcaaag tcaatcctcg taaaggtgta ttaagctggg aagaaataaa ggcttctgaa
    21181 gtgcctaaaa atgcggcaga aaaaatcgat aattagtaaa tggtcttaaa attttattag
    21241 ataaaa
//
LOCUS       DANKHL010000030        20949 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000030 DANKHL010000000
VERSION     DANKHL010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.032"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(136..1509)
                     /locus_tag="PW101_000772"
     CDS             complement(136..1509)
                     /locus_tag="PW101_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2513196.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(1509..1904)
                     /gene="rbsD"
                     /locus_tag="PW101_000773"
     CDS             complement(1509..1904)
                     /gene="rbsD"
                     /locus_tag="PW101_000773"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDL2513197.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(1932..2849)
                     /gene="rbsK"
                     /locus_tag="PW101_000774"
     CDS             complement(1932..2849)
                     /gene="rbsK"
                     /locus_tag="PW101_000774"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDL2513198.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(2957..3970)
                     /locus_tag="PW101_000775"
     CDS             complement(2957..3970)
                     /locus_tag="PW101_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL2513199.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(4051..4650)
                     /locus_tag="PW101_000776"
     CDS             complement(4051..4650)
                     /locus_tag="PW101_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2513200.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            4836..5537
                     /locus_tag="PW101_000777"
     CDS             4836..5537
                     /locus_tag="PW101_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2513201.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            5547..8258
                     /locus_tag="PW101_000778"
     CDS             5547..8258
                     /locus_tag="PW101_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2513202.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            8709..10337
                     /gene="treC"
                     /locus_tag="PW101_000779"
     CDS             8709..10337
                     /gene="treC"
                     /locus_tag="PW101_000779"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDL2513203.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(10397..11578)
                     /locus_tag="PW101_000780"
     CDS             complement(10397..11578)
                     /locus_tag="PW101_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2513204.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            11830..13353
                     /locus_tag="PW101_000781"
     CDS             11830..13353
                     /locus_tag="PW101_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDL2513205.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(13422..14150)
                     /locus_tag="PW101_000782"
     CDS             complement(13422..14150)
                     /locus_tag="PW101_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2513206.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(14163..15443)
                     /locus_tag="PW101_000783"
     CDS             complement(14163..15443)
                     /locus_tag="PW101_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2513207.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(15584..16186)
                     /locus_tag="PW101_000784"
     CDS             complement(15584..16186)
                     /locus_tag="PW101_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL2513208.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            16431..16637
                     /locus_tag="PW101_000785"
     CDS             16431..16637
                     /locus_tag="PW101_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513209.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            16949..17668
                     /locus_tag="PW101_000786"
     CDS             16949..17668
                     /locus_tag="PW101_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2513210.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(17749..18432)
                     /locus_tag="PW101_000787"
     CDS             complement(17749..18432)
                     /locus_tag="PW101_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDL2513211.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            18651..20837
                     /locus_tag="PW101_000788"
     CDS             18651..20837
                     /locus_tag="PW101_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDL2513212.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         7167 a         3749 c         4073 g         5960 t
ORIGIN      
        1 gataaaaatg gttatgcata tgtaaattat accaaagttt ggaaagtatc acaaaaattt
       61 ataaaaataa tgatcctaaa aagaaaaaga ctgaaatatg tgataaacat ctcatacttc
      121 agtctttttc attaactatt cataagacat taggtcttca tcaaacccgc taacgagagt
      181 agtgatcatt gctacgacta gactaacagc cagtcctgct aagtaccaga aaaaagtacc
      241 ggaaatgaat gcaggcaatg tggtcagact accaaataca aaagcgatcg ctttcacttg
      301 ggcaaatccc attacagctc caccagcagc tgcaccaatg caagcagcaa caaatggttt
      361 tttcaatggg atcgcaattc cgtaaacagt tggttcggtc actcccgcaa gtaaaatcga
      421 agcaaaagca gaaccggaga tcgaacgggt ttttgaacgt ttacttcgca gaaatacacc
      481 taaagctgca ccagccattc caaaattggc tgctcctgtc aacggaacga ttcggtcgaa
      541 cccataagca gagatattac tgatcatgac agggtttaca gcccattgga taccaaatat
      601 taccaaaata ttccaaccac cagcgatgat tgcgccactg atcggtccgt taatatccat
      661 catccaagta attgcggcag ctaaaccttc gccaaggtaa acacccgcag gcccgaatac
      721 cattaacgtc aaaggaaccg tgataactaa tgaaagtaaa ggtacaaata ctaattgcat
      781 attttcgcga atatattttt tgaggaattt ttcaacaaaa gaataaaaga aaatacctaa
      841 aatcgccggc atgacagtag acgtatactg catcagtact acaggaatcc ccagaaaatc
      901 agtagctgct ttgccactgt ctgaaagtaa agaggtcaga ttcggttcaa tcagtgctgc
      961 agcaatgaca gccgcaatat atccattcac tttaaacgtt ttggcagcag aaaaagcaag
     1021 aaagattggc agaaaataaa agacggcatt cgacgcagca gacaaaatat gataagttcc
     1081 gcttgtttca ctaagccagc caatcgtagt cgctaaaaca accagacctt ttaatatacc
     1141 agaaccggca aacagcggaa tgatcggcgt aaagatttcc gatatcaaac caaatagttt
     1201 ttcgatcgga tttagctttt ctttcggagc tgaatgatcg atttgacgcg tcaattcctt
     1261 gtaagcagga gccaatccct gatccatgat tatttctgca tatctatcga gtaaaatgaa
     1321 atacttaatg gaagggattt gctgttttaa tttttctgca ttggctactg gctgtttttt
     1381 caatttgata cgaagccgag tcgcacatgg aaggacagaa tcaatattgt cttttcctcc
     1441 caactgttca atcagcatat gaatctgctc ggacagtacc atctgttcag gattcattgt
     1501 cgtttccatt agaagaccac tccactcact aatataatat tggcgtaagg tgtcatttcg
     1561 cctgtacgaa taaatgcatg acagttattc aactcttttt tcatttcttc atgtgggata
     1621 aagctaatcg gtgtatctgg taacaataat ttgatttttt ccaatatttc cgggttcatt
     1681 gtctttattt cttccgccaa ttcaatcgat tgtacctcaa gttcttctaa aacgttttct
     1741 aaaacagata aaaaacttgg aagaccgttt gagacagcca aatctatttt ttcagtacca
     1801 aaggggacag gcatgcctgc atccccgata cttaatttat caaagtgccc catttgtgcg
     1861 attactcgtg aaatatctga attgattact tttccttttt tcatgtttgt ttcttcctct
     1921 cattgttaat tttagactgc tgattgttct aaagcttcgc ggtaagggat agaaggttgc
     1981 gcaccataag actgcacagc aacagaagag gctagatttc cataaaggat cgcttctttc
     2041 aagttactaa ggtctggctg aagtgtgctg cttaatgcgc caataaatgt atcacctgcg
     2101 gctgttgtgt caacggcatc cactttaaaa gcagggacga taccgtgttc tttttctgta
     2161 tgatagaaag cgccggcact gcctagtgta atgattactg ttccaatacc taattcatgg
     2221 agtttttcgg ctgctgcgac caaactgtta tgatctgtca ctctgattcc tgtaatgatt
     2281 tctgtttctg tttcatttgg tataatgata tctgtctttt caagtagttc agtaggaatc
     2341 gtttcacgag ctggtgcagg attcaggatc gttgtttttc ctgcgtcttt tgcaatagaa
     2401 aaagctagga tcgtcgcatc taagctactt tcaaactgag caataataaa gtcactgttt
     2461 gtaatcagtt gcttgttttt taggacttct tgttcatgga aagcattatt tgcccctgca
     2521 tggatcaaga tactattttc tcctgctgcg tctaccataa tcattgcttg tccggttgtg
     2581 gcgccttgga tttcagctat cccagatgta tcgatgtttt cttttgtcag tagatctaat
     2641 aactgttgtc cttcgctgtc agccccaacg cctccaataa aagatgtccg tgcgcctgaa
     2701 cgtttggctg ccactgcttg gttggcacct ttacctccgc cagcgtgaaa aatctcatgc
     2761 gcatgcatcg tttcgcctgg cttgggcatg ttggttaaac gaagggtagt gtccatatta
     2821 atacttccaa ttactgtaac cgtgttcatt ttacaaacct ccaactatct catttatttt
     2881 gatgggaaaa ttatttttta aaacgtttta gtaaaacgtt ttaatagttc gataaaaata
     2941 tagcacacct tgaaagctat ttcaagggtg cagttgaaaa tcttttttct aatcgtacag
     3001 gtaatctttt ttcttcccat tctttttctg ggaattggat ccgatctaac aataacttgg
     3061 cagatgtttg tcccaattcg aagattggtt gtgcgatcgt tgtcagtttc ggcttgatat
     3121 actcgcacat atctatatta tcatagccaa taatgctgta gtcttcaggg atgctttttc
     3181 cagcttcctc taatccgcga tataatccaa aagctaattc atcatttaaa gcaaacacac
     3241 cagttgcagc agaatcaaga agttctgctg tgatctggta gccaccttgt tttgagaact
     3301 gtgtaggtaa gaggatcagt tggtcgtgag ggatttggta aacatcaaga gcagatttaa
     3361 acccctcaat ccgagcgtgg acattttccg gtggattttc aggatagaca agggcgatcg
     3421 tttgatgtcc tagactaagc aagtgcatgc ccgctaggta accaccgcga aaatcgtctg
     3481 ttcgtacggc atcactaaat ccttcagatt ttttctgatc caaaacaatg aagggccggc
     3541 cttgtttttt caaattttcg ttgattgcat ctgtagagac agcagaagtc gcgataatga
     3601 atccatccac acctcgacgt gtgagctcgg ctagatattc gatttctttt tgatgatcag
     3661 aatcagcatt acataatatc gtgacgaaat tctgcttata caagatatcc tcaattcctc
     3721 gcatcagtgt actgaagaat ggattcgtga tatcaggaac gagtacacca attgttttcg
     3781 tttccttggt aatcatttgt ctagcaaaat agtcaggctg atagcctaat tcttctttgg
     3841 cagctaaaac tttctgaaca gttttcggac tgaatttctc cgtatttcca tttaatatca
     3901 aggagacagt tgtgattgaa acgccggaaa gtttagcgac ttctttgatt gtgatctttt
     3961 ttttcaccat gttcatactc ctatctaaat cctgtggtta ttttcccatg aaatcaaaaa
     4021 gaagtaaatg aaaatcacgt gtgccagtac ttatttgctt tgttggatag cttcgattat
     4081 ttgtttcgtt gagcgtactc gtgcagtttt cggaaatatc ttttttatga acaattgatg
     4141 ggattctttg tcacgatcac tgcaagcatc ttcagcaagc acgatatggt acccgtgttg
     4201 aaaggcatct aatgcagtag catatacacc gtttgttgtc gctacaccgc ttaaaatgat
     4261 agtttcgatt ccacgccggc gaagttgaag atcaagagaa gtaccaaaaa aagcacttgg
     4321 attgtatttt gtgatagtca acatattatc tgtatctttc aaagaatcct tcaaaagtaa
     4381 atcagtgaat tctggaggta cagaagcagt cattccaccc atatcagttt gttgactcaa
     4441 ataagggaaa gtggagatgt ctacgttcac taaaactttc aaggcagcgg tacgtttcaa
     4501 tgttcttact aacaaatcat tgtttgtcag aatagatgct gcagtgtgtg gagagagtgt
     4561 accgttattt acgatctctt tttgcatgtc gatcgcgaca aaagcagttt ttgataaatc
     4621 aaaaatcata ttttccttta aatcattcat gatgttcctt ctttcaaata acaatgtttt
     4681 ttatgtaagt aaatcataaa tcgtttattt gtcaactaaa ttaaaggatt cctttcttta
     4741 gtctagagaa attcagtggt aaaataaata agtgtgagaa aacgcattcg atgcatagat
     4801 atagcgagaa atatcgtgaa aatggaggaa tcagagtgaa gtatgtcgaa gtaaaaaatg
     4861 aatacaaacg atacaaaatg ggagaaacga ccattacagc aaatgacgat atttcttttt
     4921 caattgaaaa aggcgaatta gtgattatac ttggtccaag tggagcagga aagtcaacga
     4981 ttcttaatat tttaggtggg atggatacac ctgatgaagg tcagatcatt attgatgaca
     5041 cagatattgc gcaattttca gataaacagc ttactgctta ccgacgaaca gatgtagggt
     5101 tcgttttcca attctacaat ctcgttccta atctaacagc aaaagaaaat gtggaattgg
     5161 caacggaagt ttctcctgat gcgcttgatc ctgtagaggt attgaggcaa gtgggcctag
     5221 ctcatcgatt gaataacttt ccgtcccaat tatcaggagg cgaacagcag agggtttcaa
     5281 ttgctcgtgc actagctaaa aatcccaaac ttcttttatg tgatgaaccg acaggcgcgc
     5341 tggattttga aacgggcaag caagtattga aattattgca aaacgctagc agacaacacg
     5401 gcaataccgt tttgatcatt acacataatt ctgctttagc gcccatcgct gatagagtca
     5461 ttcacattaa tgatgctaaa gtaaggtctg ttgaattaaa tgatcatcct tcatccattg
     5521 atgagattgt atggtagggg gattttatga aaacaagaac atatcttaaa gcaggttttc
     5581 gcgaaattcg gcaatctaaa ggacgattca ttgcaatcat ccttattgtc atgctaggaa
     5641 cacttttatt tgtaggtgtc aaaactgctg gacctgtcat gcaaaaaacg atggatcact
     5701 atgttggtac agcaggactt tctgatttgc aaatcgtttc tactggtggc ttgacacaaa
     5761 aagatattgc tcaagcagaa aagatcccag atgcaaaagt agaaacagga aaacagatct
     5821 attattccaa tactggcaaa aacgaagtga ttcaaatatt ttcttataac aaaaataaca
     5881 agcagaacaa gctacaatta actgatggaa aactcccgga aaaaccaaac cagcttgtat
     5941 tggatgaaaa agcggaagat gaagggtata aaatcggtga tatttatcag attgattcag
     6001 atgaattgaa agaaaaagag tataccatca cgggctttgt ccgttctcca ttgttcatca
     6061 acaatttgga gagaggatac gcgaacgtcg gaaatggtag cgtcgattat tttgtctatt
     6121 tacctgaatc cgtttttaaa acagatattt attctgtctt atatcttgat tttacaaacg
     6181 taaagaattt gaatacgtac agcaaagctt acaaagataa aatggagcaa aatcaagaaa
     6241 aggcagaaaa gtatttacaa gatcgacccg aagaacgctt agaagagctg aaaaaatcgg
     6301 ccaacgaaga attagaaccg gcacagcaaa aaatagcgga tggcaaagca caaacaacgc
     6361 aagcaagaac acggttagaa gatgctaaaa aacagttagc gcaacagtca gcaactatcc
     6421 agcagcttcc tgaagcgcaa cgaacaatcg ctgttcaaac attatctcaa caagaagctc
     6481 aacttgctga acaagaaagg caattagttg agaaagaacg tgaactggcc tctgcagagg
     6541 aagaattgaa tcgaaatgca gaaaaaattg aaaatttaac taaaccatca tacctataca
     6601 atgaacgttc agaaaatgtc ggttttcaag aatttggtga tctgtctgat cgtatcgctg
     6661 cgattgccaa tgtttttcct gttttctttt tctttattgc cgcattgatc acctttacga
     6721 caatgacacg tatggtagaa gaaaaccgca gggaaatcgg gacattaaag gcgttaggtt
     6781 atacgaaagt tgaaatcgca aaaaaatatg caatctatgc aagtctagcc tcagggatag
     6841 gtattgtttt aggaacgata ttaggaacga atcttttgcc aaggatcatt tttgagctgt
     6901 ctaacgagcg gtatgatatt gggagtgcgc ttatttttta tgactggccg ccgattttac
     6961 aagcagctgc cgcattcttc attgccgcat ttggcgctgc aatgatcgtt ttattcaaag
     7021 atttaagaga acggcctgct gcattgcttc aaccgaaagc accgaagcca gggaaacgca
     7081 tttttcttga atacatcact ccgctgtggt cacgccttag ctttaatcaa aaaatcagtt
     7141 accgaaatct cttccgatat aagtcaagaa tgcttatggc gattatcgga attgctggat
     7201 gtgccgggct gatggttgca ggcgtgggat tgaaagattc tctaagttct gtttcagcca
     7261 aacaatttgg accgatcatt gactatcaag caatcgttac attggattca tccacggaag
     7321 atagtcgtca aaaagcagcg gatatgctgc agaagcaaga aaaagtaaat gagatcctag
     7381 ctattcatac tcaaaccgta gaattgagaa aaaaaggaca tgcttcgcaa agtctgacag
     7441 tgatcgtacc aagtaaaaca gctgatttgg atcgttttat ccatttaact gatcttgaag
     7501 agaacccacg ctccttgcct aaaacaggaa tcgctattac gcaaaaagta gcagaactat
     7561 ttgacttatc tacaggagat catatttcgc tttatgatga agatcaaaaa gaatggaaag
     7621 tcgaagtaaa agaaatcgtg aaaaactatc taggtagctt tatttatatg tcccctgaat
     7681 attatgcttc agttactgat caatcgatga tggataatgc atttttgctg cgtacagata
     7741 agatgacaac aaaagaagaa gaaatgcttt ccgagaaact gcttgccaca gacgctgtga
     7801 caaatacgtc tttcatgtcc aaacaaatcg aaacgcaaga agaatcaatg gcgaacttag
     7861 actcggttgt cttaatcttt gtcgtattat caggactgct ggcttttatc gtcttgtata
     7921 atctaactaa tatcaatatt tccgagcgtg tacgcgaatt atctacaata aaagtccttg
     7981 gattttttga taaagaagtc acgatgtaca ttgttcgaga aaatatcatc tttactcttt
     8041 taggaatcat cggaggattt ggtgtaggat atatattgac tgacttcatc ttgcaacaag
     8101 cgtctatgga gaatgtcatc tttccattag tgatcacatg gtgggcttat gtcttatcag
     8161 caggattaac tatcgtattt acggttattg tgatgattgt tacccatttc aaattaaagc
     8221 atatcgatat gattgatgcg ttgaaatcga atgaataaaa acagaggttg tgaaatcagc
     8281 ggtttgacct gagtattaga gaaacaaacg gcaaaatagt ttttcatatt ttgccgtttt
     8341 ttgagctaat accgaaggac agctgattga acaccatgaa tcagaggttg taaaaaagcc
     8401 gtcctgcatc gagcaaaaag aatactcaaa taaaattagc tcctcatgtt ttacatttga
     8461 gaagactgat tgccgtagat aaaagctttt gaacaccatt tatagaagaa acaagaatat
     8521 tttgctattt taaacaagag gatagacaaa tacatgtctg atctcttgtt ttttattggg
     8581 caacagacct cttagaatgt ataaagataa attttacaaa cgctttcggt tcagttataa
     8641 taataaatga taacttgtat atataagttg tggtaggtaa taaatcagtt gaatggaggt
     8701 aaatatccat gacatttaaa gacaaagtca tttaccaaat ctatcccaaa tcttacaaag
     8761 acagtaatgg tgatggaatt ggagatttaa aaggaatcaa agaaaaactc ccgtatctga
     8821 atgatctagg catcgacatg atttggctaa accctattta tccaagccct caaaaagaca
     8881 acggttatga tatctctgat tacacagcca tcgatcctat ctttggtacg atggaggaat
     8941 tcgaacagct aatagccgaa gcaaagaaat accggataga aatcatgctc gatatggtgc
     9001 tgaatcacgt ttcgacagaa catgaatggt tcaaaaaagc actagcaggg gaccagcatt
     9061 atcaggactt cttcatttta agagaagagc caacggattg ggtatcaaaa tttggtggaa
     9121 gtgcttggtc gccatttgga gatacgggga aatattattt gcacttatat gatcggacac
     9181 aagctgactt gaactggcgg aaccaagcag taagagagga actattcaaa gttgttcgtt
     9241 tttggatgga aaaaggggta aaaggattcc gtttcgatgt tatcaatgtt atcggtaaag
     9301 acgaacaatt gaaaaataat aaagaaaatg atggcaaacc tgaatatacg gacaaaccaa
     9361 tcacccatac gtatctaaaa gaattgaacc aagcaacgtt tgggacggat tccactatca
     9421 tcacagtcgg tgaaatgagc tctacgacga tcgaaaattg tatcttgtat acagaaccta
     9481 gtagagaaga attatcaatg gtctttaatt tccatcattt gaaagtcgat tatgaaaatg
     9541 ggaataaatg gtctactcct ccatttgatt ttgcagaatt aaagcgacta tttcatacat
     9601 ggggagaaaa gatgagtgag ggtaacggct ggaatgctct attttttaat aatcatgatc
     9661 agccacgtgc gctcaatcga tttgtagcta tcgatcagta tcgaaaacaa ggagcagaga
     9721 tgctagcaac catgatccat ttgaaccgtg ggacgcccta tatttatatg ggagaagaaa
     9781 tcggcatgat cgacccagac tattcatcga tttccgacta tattgacgta gaaagtttga
     9841 atgcttataa gttactcctt gaacaaggat tcacagaaaa agaagcgttt cgtcggatca
     9901 aagcaaaatc tcgcgataac tctcgaacac ccatgcaatg gtcagacaca gaacaagcag
     9961 gatttactac agggaaacct tggttaaaag ttggaggcaa gctagaagag attaatgtag
    10021 aaaaagaact atcttctgaa gattctattt tgtcctatta caagaaattg attcggctga
    10081 gaaaaacata tccaatcgtt gcacaaggag attatcatgc ttatgaagca aatcatccgg
    10141 aagtttatgg ctttcttcgc caatttgaag gacagcagct gcttgtatta acaaatttct
    10201 atgataaaga tacaaggatc gccatcccag aaggattcac agaagccaat attcttatcg
    10261 ataattattc cgatactgtt cttcatcaaa gtctggagct aaaaccatac caggcaattg
    10321 ctttgctgaa aaattaaaac aaaaacacaa aacgtcgttc ctacgagcta ttctcgtaag
    10381 atcgacgttt tgtgttttat ttaaaataat cttcttcaga aaatgaagag ttttcatact
    10441 ctcctttatc gccataaaat ttattgtagc gttgtttgaa tttacggaaa ataaaagtaa
    10501 cgagtaattt gactaacgta tagataggaa taccaaaaat aacgcccaca attcccatta
    10561 gatcacccat gacaagtaaa acgattaaga tcgtaatcgg atggatctgt aaacgatcac
    10621 ccattactcg aggaatcacc aagtcaccgt ggagaagttg tacggcaaac cagacaatga
    10681 caaatttgac agccataaaa gtagagtctt ggaatgcaat gatcaaacct ggaaagaagg
    10741 cgataaacgg cccaatatat ggaatgatac ataacaaccc agcagaaata gctaaaacac
    10801 tagcaaattc taatccgatc agtaagaaac aaacccagta gactgctcca aggataaatg
    10861 aagcaatgat ttgccccttt agaaatgaac ccaattgttg attagcaatc tttgtcaaat
    10921 ttttaaaatc tgtacgaaat gctggtggga caatcgctaa aactgactga taaaattttt
    10981 gaggatctct caaaaggaaa aaagcgacaa ttggaccagt aaataaagta ataaaaatag
    11041 ctgtaacagt tgaaaaaatg ttgctgatcc gttgtgcgcc actttcccaa taatttccaa
    11101 taaatccagc tatatcagac gtcacgtcgt caatagaagc gaaaaattga ttaaatgaat
    11161 cggcaaaagg tgttttctcc aaaaattgtc ggagactctc ttgaaaatct tgaaacaaat
    11221 ctggcaaact actgatcaat gtctttgcct gttcttcaag aaaaggaata agccatatgc
    11281 ttccaagccc caataaaaat aaaataatca cataaacgat aatgactgac aagttcctcg
    11341 aaaggtgctt gctcaaacgt ttgatgactg gttccaaaag ataatacaag atcagtgcaa
    11401 acaataatgg gggcaagata gtagcgataa tgattccgac aggcttgaat ataaacgaga
    11461 tctgatggaa aaaataaatg atcagcgcaa gtaagataac tattcctaaa acatagtaag
    11521 aggtttttcc tcctaaaaag cggatcaaac gattcgttgt atctgtttct tttttcactc
    11581 tttttcctcc tttatctata acttcattat agacaaaagc gggtataaaa ggaaaatgat
    11641 agaggttgat tgcaaaaata tcgtcaatca ttcatataaa ctttctcatc aattaaaata
    11701 agaaaacgct tgacagttct tttaaccata tgataaatta aatcctgttg ctaaaacgta
    11761 actattacgg tttgcgaaaa gcgaatcgga acattcaaat gaaagaagat gagaagagga
    11821 gtgtcataaa tgaaacgatt gataggtata ttggccatgc tggttttagc aggtatgtta
    11881 actgcttgcg gggcaagtgg gaaagccgag gacagtaaag aaaaattgag cgtgatgacg
    11941 accttttatc ccatgtatga ttttacaaaa gcaatcgtcg gggatgaagg agaagtagag
    12001 ttattgattc cagcagggac tgattcccac gattatgaac cttctgctaa agacatggca
    12061 aagatccaag atacagatat ctttgtttat aatgatgaaa acatggaaac atgggtcccc
    12121 gcaatccaaa aaacattaca agaaggcaat gtccatacga taaaagcgac agaaggtatg
    12181 cttttacttc cgggaagtga agaaggacat gatcatgacc atgaacacgg ggaagaaggc
    12241 catacacatg agctagaccc acatgtatgg ctagctccaa gtttagcaat caagcaggta
    12301 gcaaacatac gtgatcagct gattgaagct tacccagaaa aacaggaagt atggacaaaa
    12361 aatgcagcag cctataccga gaaacttcaa gcactccatc agctttatca agaaaccttt
    12421 aagcaagcaa aacaaagatc ttttgtcacg caacatgctg cattcaacta tctagcgtta
    12481 gaatatggat tgaatcaagt atcgattgct ggtctttctt ctagtgaaga accgtctgcg
    12541 gcgagaattg ctgaactgaa acactttgtt aaagaacatg gaatcaacta tatttatttt
    12601 gaagaaaacg ccaaagatag tattgccaga acgttagcaa acgaagcagg tgtatcttta
    12661 gaagtattga atcctttgga aggattgacg aatgaacaaa tagaaaatgg agaaaactat
    12721 ctttctatca tggaagcaaa cctcgaagcg ttgaaaaaaa cgacagagac agaaaacccg
    12781 ctagaagata ctttgacccc caaaaaagaa aagagcgtct acaacggtta ttttgaagac
    12841 gaggatgtac aagatcgctc tctttcagat tgggcaggaa cttggcaaac agtagatacg
    12901 tatgtagaag atgggacttt tgatcctgtt tttgaatata aagaaaaatt gaatcaggat
    12961 aagacagcac aagaatataa agaatattat acgaagggct atcaaacaga tattgcttca
    13021 atcaagatca gtgagaaaga gatggcattt acgtttaaga atggaacaac gaaacgttca
    13081 aattatcgtt atgtagggaa agaaatcttg acctacgagg caggaaaccg tggcgtaagg
    13141 tatctattcg aagcagaaga taaggaaagt ggcgctttta gatatattca attcagtgat
    13201 cacacgattg caccggctga ctccgaacat tttcatttat atgcaggaaa tgaaagtcaa
    13261 gaagtattgc tgaaagaaat ggagaattgg ccaacatttt atccagatac aatggatgga
    13321 cgtgaaattg cacaggaaat gcttttgcac tgataggcaa aaaagaaccg aagggtttgg
    13381 atcggctggt catttagtcg gtccaaacta ttcggttctt tttatgaaat caatttcttt
    13441 aatccacgga tagaggctgg gatggtctcg ccgtttgcca acgtgacttc tctggttttt
    13501 ttattaatcg accgcacatt ttgtgtattg ataacatagg atttatgaca acggataaaa
    13561 ttccgatgca ttttctcgat ttctttgatc ttctcataga attgtaattg ggcatgagtc
    13621 aaatggactt ccagtttatg tggcgctcct agcgaagtag aaaaatattg gatgtctttg
    13681 atcggtaaaa atttgatctc atcattaaac tctattttga agatatcttt ttggatttgg
    13741 gacatttgaa aatcttcgaa aattttgctc aggatatgct cgacattttc ttttaaatca
    13801 taaagatctt ctttgacgat ataatcagtc ggttcgatgt tgctttttaa gattttcatc
    13861 gacatttccg tatgggaagt cacatagatt atttttccta aaggatctaa ttcacggatc
    13921 aatttaccga tcgctacacc atccatttca ctgtcttcta gttcgatatc taaaaagtaa
    13981 ataccaaaag aagaagttct gatcttggct gcattgagaa gttcttccgg atcagaggta
    14041 gaaatatgga cataactgtc taagttttca atcatgattc tatttttgat caccgttgat
    14101 aaaacttcta attgttttgg atcgtcttca caaatataaa tgggaatcat atcattactc
    14161 ctttatttta tttgtatata aatcgtttga gaaaaacgct ggttttcaat tgatgtttca
    14221 aggaaaagat aaggattggt ctttgtcaat tcatccaaga taaacaagcc aaggccttcg
    14281 ttttttggat ttgtttttgt tgagaaccct ttttgtttca actggtttaa cggtaatgta
    14341 tttgttgttg tattgatcac acgaatcatc acggaatgat tcttcttgag tatggtcaat
    14401 tcgatttttt tcttctttgc agcactagct ccttcaatcg cattgtccaa gataatacca
    14461 agcatacgta caaggtttac cgtatgtttc gtatccagtt cgattggctc aggaaaatga
    14521 agctggacga tcaacccttt ttcctgtgcc atcatgagtt ttaatgacag taaactacgg
    14581 atctcaggaa ctttcatatt atccaacata cttagattgg ccggaaaaag gttcatcatc
    14641 tggcgtgtag gtaatatcgt ttgatcataa taagaccgta atccttctaa gtcgccttct
    14701 gaaatatagc tgtgaagaga aaaaagcaga ttttggtaat catgtttgaa ttctctcaag
    14761 tcttgatagt ttttttctag atctttgata taatcctgtt gcatcctgac ttgttgctct
    14821 aataactgat cagctaattt cttattagag attttccaat aataaaccaa gatgatcagg
    14881 ccaacacaca gatatagaat gaaaaaactc gtattcaaag ctaagacatc aggtgtttca
    14941 ccagaaaaac gggtataaaa aataatgata tagtatgtaa ataatatcaa tgtcccgatc
    15001 gtaccgatca tacggcggtc cacagtgtga aaccaagtct ctaacgattt ttttaaacaa
    15061 aaagctaata aagcagataa aaaagcggat aagacaatgt gaaaatactg caatgaatct
    15121 cctggttcaa taaccgaaga tttcaaaaca aaaaaatcgc cgatgcttgt taagtgatct
    15181 cctaaaagcg acgtcaataa cccaaaagga agcgcgtaaa ccaattgttg acgcgaagtc
    15241 gaaaagaaga aaaaagtaag catcagcatt cctatagcaa ttagagcagt aaaaatccct
    15301 aaaatcgaat agacccatcc aatcaaaata caagaaatca gaatgactcc cgcaaacaga
    15361 tatttgtatc ggatgtgatg gtcaatcaat aagatacaaa ttgtgtaaat caaatattgc
    15421 aacgctatgg accataaaaa cataaagtac agctcccatt gatttttacc aaattatagc
    15481 acaattggcg tattattttt ttaatttttc agggattttg aattcagaac tcataccaaa
    15541 gcagcagcca gatacaaaaa agtcagataa gttagttaat tgtttaatga ttgttttttt
    15601 catgaaagat tcctccaaaa ttcatcaagt agtttgtaat aggaagcaac gtagctattt
    15661 ctaaaacaat cccaagtagc aaataggtag aaaagtaaga agctgagaaa aataacgtaa
    15721 tgagagaaaa aattagaaac atgctgatcg ccttctttaa taatggggga cgtttctcgg
    15781 gttcaatggg attgattgct gtacctcttg atccatagcg aagcagtaac aaaaagcagc
    15841 tgattaacaa aaagaccatc attgggaagg agatagtcaa ttgcagatgt ttcatcacca
    15901 agggaacgcc ccataaataa agtagtgtcc acagcagaca aacgaattca gagtcggaat
    15961 gcaatccata agcatatctg cggatgcaga tataagaaag aaaggtgatt cctgtttccg
    16021 cccatgagcc tgtcaccaga gaaaagacta gaacgaccgc tagttttcca agattattca
    16081 ataaaacttc taagacaaat tttacttgga catacaccat atcttcttcc gatttgtctc
    16141 cgctgaataa ggcttctaat aatttgttca caactttatc tttcatctca tacgtacctc
    16201 caccacaaat tttatgctaa aaaaaataaa aaacgtcttt tgtttcataa aaagtggttt
    16261 tgacatcaaa accgtcatga tctcctaaaa aaatcttcgt tttccaagaa ccttaccaaa
    16321 aaaagtgaca gttttgatac gaaaagtact ttttatgaca agtttgtgct ttttcgagtg
    16381 atttatgtta aaaaattact gaccttataa aaggtgatgg agggattctt atggaaaatg
    16441 aacaattgtt tgaagtcggc gaaacatatg catttaaatc tgatttattt gaatcaccaa
    16501 taaaaggaaa aatagaaaaa atctatgaaa atagtgtgat gatacgtgtc atgaaatgcg
    16561 cagtagaaga caatgatacc gctcataatc tgcactggca gttagttgta cgcaaatcaa
    16621 gtacaataga agaataaaaa cagataaaat gttgagagca taaagaggtt gtgagtctcc
    16681 cgtttagctc tgagttacaa ggaataatgt taaaaaacag cttttcatgt tttatgaata
    16741 ttgccttgtg accgaggtgt tggcaggaga atactgttta tttgaaaaaa gggctgcgac
    16801 aagacttttg tcacagcctt tattttcatg aataggagtt acttttggct acttttagag
    16861 aagtagcttt taaggagcag ataaagtcaa ggcttttatt tctataaatg aagtagtaaa
    16921 atgaagaaca gattttatta caggaaggat gccgataatt gtgatcattg ttgcaggaac
    16981 gatcggtgca gggaaaagca cactaactga aatgcttgcc caagatttag aaacaaaacc
    17041 tttttatgag aacgtggaag ataatgaagt attgccgcta ttttattcta atccagaaaa
    17101 gtatactttt ttattacaga ttttcttttt gaacaaacgc tttttggcaa tcaaagatgc
    17161 tttcagccat gatgacaacg tactagaccg ctccatctac gaagatagta tgctgtttca
    17221 tttgaatgca gatttagggc gtgtttctga agtagaagta aagcagtatg aaggtctttt
    17281 agaaacgatg ctgaaggagt tagaagaaat ttcgccacaa aagaaaccag atctactggt
    17341 ttatattcgt gtatcctttg aaactatgtt agcgagaatc aaaaaaagag gccgggaata
    17401 tgaacagcta gaacaagatc ctgaactcta ttcctactat aaagaactca atcgacgata
    17461 cgaagagtgg tatgaacagt ttgatatatg tcccaaaata gtgatcgatg gggacaaata
    17521 tgatttcgta gccgatccgg catgtggaga acaaatcgta caagaaatca agatgcgggc
    17581 aaagaaaatg acagaagagg cagatgccca gtcgatccgc tacactacaa ataacccgaa
    17641 agcaaaggga atctttacaa ctaaataata taaaaactaa gcaaattgct cactcgcaga
    17701 tatttctctg tgagcagcat cttgcttagt ttttttgatt cattgagatc aaatagtgaa
    17761 ccgattcgta tgataaaatt gccataaatg aaatgtctga gtgagggcca tgatcaaaaa
    17821 taaaatggaa acgatcaaaa tcgaatgggc gccaaacaga tgatgacaga aaacaaggac
    17881 aatagctcct gcgataaagg caagaacgac acttagatag tggaaaaatc gatcaaaatc
    17941 tgctttttct ttagaaatac aataatcata taagttcgtt gctacccctt ttaaatttcc
    18001 agttgtaaat aaattagtgt aggcacgccc gttgatttta tcaaaagcta cccactggat
    18061 cgatgcagta aaagcaatca aaatcgttac taatgcggga tgctcatgga cgggtgtcaa
    18121 actaacaaag aaaaaaatca ctgcttcata atatagaaca aacaagcgcc agaaatgttt
    18181 ttcttttttt ctaaaataat caattagata ctttgttaga agaatcccta agaaaaagaa
    18241 aagggtagac aatacttttt taccaacact tagccattct ccgtcaaatg cttggatgat
    18301 cgatagaatg atattgccag tttgagcaga ggcaaaagca ccatattgga tataagtata
    18361 actgtccatg gctcctccga taaatgtcag caaaagtcct attgtacgcc cctcgtgaaa
    18421 ggaagtctgc atttttttca tcacctttta tgaatatttt tgtcttcctt atcttagcat
    18481 aaacgattga aaaaaaagga aaaattttca gaaaactaaa atcatatcgc ttcccatttt
    18541 catttttaga aaggtctgtt agtataaaga aaaaatagac aacgaaactt taaataacca
    18601 acttgaaata aacaaattca aagaagaaag atgggaagga aatcaatcag ttgaatgaaa
    18661 aagaaattct ccgattacaa aaagaattga aacgctggga gaacaagtat aaaaagcttc
    18721 aacaagtctt tcggctacaa gaagaaatca ttgctaccta tgaagcggct tatggcgaag
    18781 cacccggact tattgaggaa gaagtacaag taaaagaaag tgaaggaaac agctctttct
    18841 ttaaaatgat ggagaaacgt tcctataatc gcttcaatca agaacaaaaa gcagctatca
    18901 catatgatat gaagaaacat cttcgaatca tagcaggagc tgggagcggc aaaacgcaaa
    18961 caatctgtgc aaaagctgtt tatctgatga cgcaaaaaca agtagacgaa gaacgcatac
    19021 taatgatcac ttttacaagg aacgctgcaa atgaactaaa aaaacgagta gataatttca
    19081 gccagagaaa aaccgctgtc catatcggaa cattccatag tatctttttt aggatataca
    19141 atgaaatttg tcgaaaattt cctgaagtag caatgcaagg aatacaagga gacttttcaa
    19201 aggatactgc acaaaaagtg aatgctgttt tgcaacagct gatacgtaaa tataatcttt
    19261 atatatttga tcaatatgga gaaaaaacga ttgcttctcg gttagactat tggcagaata
    19321 tgaacctttc agtagaagaa atggttcagt tagttaaaga aaaatatgat tccattgata
    19381 agaatgcacg acagccgata tcagaacgac tgtatggatt gctcacggaa ctccaagaac
    19441 aaaaaagaag ccagcaattg ctagagttca atgatgtact ccaaaatctg aaaagcgctt
    19501 tggagaatga ggagatccag cattatatcg gccaaaaata tgattatcta ttcattgatg
    19561 aatttcaaga tactaatcca cttcaatggg aaatcgtgaa acggatgaca aaggaaaaca
    19621 ggatcaagtt gattgttgtt ggagatgatg atcagagtat ctatggattc agaggatcag
    19681 agccagcata catcaagaac tttgaaaaag aatatcctac caaaactctt ttcttattaa
    19741 ctaattatcg ttctcgggca gctattgtcc aaggagcaaa tagattgatc agctataata
    19801 aaaatgatcg gataccaaag gcgatgattc cagcacaaaa agaagcaggt gttatggaag
    19861 cctatttgtt ttctgatact aaagcagaaa gtcagtggct aatcaaacaa attcagacat
    19921 ttattcaaaa aaatgggaaa tataaggaat caatcatatt ataccgatca gcttcacaaa
    19981 cgcagcagct tgtccaatct ctgctgaaaa cgaatattcc ttttgtactg gaagcagata
    20041 gtccatatga aggtatcttt ggtatcaagt ctttccaaca tttctatcaa aaaattgttc
    20101 attggcaaac agcactgaat ctgcaaaaga aaaatcaggc ctaccagcaa ttgctccggc
    20161 agctgatggc agactgctac ctgaaaaaaa gtgaatgcga tcaatacttt tctcctaaat
    20221 atcaaaatat agccgttgca gattatattt tgtcgataag ccccaattta aaatcgaaat
    20281 ctgcagaaat ccgagagttt gaggaagcgt tgcgtaaaac agaccagcat caagaaatct
    20341 attcgcttgt tcaaagctat cttcatctgc cacgtatagc taaggaaatc gaccagagtg
    20401 atcaagaatg gatacgagaa gaagtaaagc agcatgctac tttcagaaga ctccaagcct
    20461 tatcgcaaga aacccaagcg acagctaaag aattaaaaac ccgcttgatt gcttacagac
    20521 aaggaaaact tgatgcacta tgtatccaga gcattcacaa aagtaaaggt cttagttacc
    20581 gaaatgtctt tttgattggt tgtaacgaag gaagtttgcc ttataacgga gcgacagaaa
    20641 agaaagtaat cgactttaat gaaataaaag cagaaccagt aacgacgata gaagaagaac
    20701 gaagactatt ttatgttgcg atgactagag caagaaatcg tctgtatcta tgtgtacctc
    20761 aaatgaaaaa ttcaagaaaa ttgaaagtat cgagatttat caaagaaacc gggagtcgtg
    20821 tgaaaaaagg aaagtgatag aagagagggt gtgacagaag tggtcagttt caagaaagaa
    20881 gaaggaatgt acgaaaattg cttctcaaat ttttgtgaaa tttcgactta tttttcgaag
    20941 aagctactt
//
LOCUS       DANKHL010000031        20213 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000031 DANKHL010000000
VERSION     DANKHL010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.033"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(41..2116)
                     /locus_tag="PW101_000789"
     CDS             complement(41..2116)
                     /locus_tag="PW101_000789"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDL2513213.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2118..3035)
                     /gene="glyQ"
                     /locus_tag="PW101_000790"
     CDS             complement(2118..3035)
                     /gene="glyQ"
                     /locus_tag="PW101_000790"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDL2513214.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3419..4231)
                     /gene="recO"
                     /locus_tag="PW101_000791"
     CDS             complement(3419..4231)
                     /gene="recO"
                     /locus_tag="PW101_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDL2513215.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4374..5273)
                     /gene="era"
                     /locus_tag="PW101_000792"
     CDS             complement(4374..5273)
                     /gene="era"
                     /locus_tag="PW101_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDL2513216.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5288..5683)
                     /locus_tag="PW101_000793"
     CDS             complement(5288..5683)
                     /locus_tag="PW101_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HDL2513217.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5667..6143)
                     /gene="ybeY"
                     /locus_tag="PW101_000794"
     CDS             complement(5667..6143)
                     /gene="ybeY"
                     /locus_tag="PW101_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDL2513218.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6160..8352)
                     /locus_tag="PW101_000795"
     CDS             complement(6160..8352)
                     /locus_tag="PW101_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDL2513219.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8376..9347)
                     /locus_tag="PW101_000796"
     CDS             complement(8376..9347)
                     /locus_tag="PW101_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDL2513220.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10053..11432
                     /gene="brnQ"
                     /locus_tag="PW101_000797"
     CDS             10053..11432
                     /gene="brnQ"
                     /locus_tag="PW101_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL2513221.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11588..12034)
                     /locus_tag="PW101_000798"
     CDS             complement(11588..12034)
                     /locus_tag="PW101_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDL2513222.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12061..12237)
                     /locus_tag="PW101_000799"
     CDS             complement(12061..12237)
                     /locus_tag="PW101_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDL2513223.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12399..12827)
                     /locus_tag="PW101_000800"
     CDS             complement(12399..12827)
                     /locus_tag="PW101_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2513224.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            12919..13779
                     /locus_tag="PW101_000801"
     CDS             12919..13779
                     /locus_tag="PW101_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDL2513225.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(13863..14213)
                     /locus_tag="PW101_000802"
     CDS             complement(13863..14213)
                     /locus_tag="PW101_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL2513226.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14229..15956)
                     /locus_tag="PW101_000803"
     CDS             complement(14229..15956)
                     /locus_tag="PW101_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDL2513227.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            complement(16169..17095)
                     /gene="trxB"
                     /locus_tag="PW101_000804"
     CDS             complement(16169..17095)
                     /gene="trxB"
                     /locus_tag="PW101_000804"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDL2513228.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17240..18676)
                     /locus_tag="PW101_000805"
     CDS             complement(17240..18676)
                     /locus_tag="PW101_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2513229.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(18673..20076)
                     /locus_tag="PW101_000806"
     CDS             complement(18673..20076)
                     /locus_tag="PW101_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2513230.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
BASE COUNT         5441 a         4397 c         3614 g         6761 t
ORIGIN      
        1 tattttgtgc ctgttctctg gtttatttac atattagaaa ttattttgtg atgatttgat
       61 ctaaacttgc tagtgacaaa gccatggcag caatttgact taattgcgtt aatcggttct
      121 tacgtactgc tggttcttct gccatgacca tcgtctgatc aaaataattt tcgatcagtg
      181 ggcgaaggct tacaagctga tcataatttt cgttgatcgt ttggtcagca aagcttgttt
      241 tcattgcctc tactgctttg tgcagttctt tctccgcttc gttttcgaac agttcagggt
      301 ctacttctga ttgtttttca agctccgctt ttttcgctaa gttcacgaca cgtgtcaaag
      361 cttccatcga tggtttgaag tcttcatctg caagatgctg cttgaacaat tgtgctgctg
      421 caaatacttt tgtcagatct ttttgttctg cattcaaaac agcttcgatg acatcatgac
      481 ggatattctt cgttgtcaat aattggcgca gacggccttt gataaagtcg atcacctgtt
      541 gttgtcccgc tgacaagcca atgccaaaac gatccgtatc tttactgata acttcactaa
      601 tcgtttcttg cagaacagac aaaggaaagg cccatttttt cgattcgatg attcgaacga
      661 caccatatgc ttgacggcgc aaagcatatg gatcgtttga gccacttggg atcatcccaa
      721 ctgcaaagaa agaaaagata ctatctaatt tatcagcaat cgctaaaaca gaacctactg
      781 tagtctcagg taaatctcct tcactggata taggcatgta atgttcacgg atcgctgttg
      841 ctacggctgg tttctcgcct ttcagcagag catatttttc tcccatgatc ccttgtagtt
      901 cagggaattc tcctaccatg ttggttacta gatcaaattt atagatttca gcagcgcgtt
      961 gcagatccat caattcttcc tcagatagtc ctacttgttc gcctatgatg cgagcgatga
     1021 cttgtacccg ttgcattttc tcatagatgc taccgatttt ttcatggaaa gtaacagttt
     1081 ttaatttttc aacgtattgg tcgattgaag ctttcttgtc ttcattataa aagaattccg
     1141 catcttctaa acgagccgtc aatacttttt gattgcctcg taccacattt tccaatttta
     1201 cccgatcgcc attacgagca gaaataaaat gaggaagcaa cataccttga tcgttccgta
     1261 cttcgaaata acgttgatgt tctttcatag aagtgactag tacttcttca ggaacagata
     1321 gatatttttc ttcgaagtcg ccagaaaaga cagttggata ttcgactaaa ttgttgactt
     1381 cttctagtaa atcttcatct agatcaatga tccagtgatt tttttcggct aattcgtttg
     1441 cttgtgtgat gatcatttct tttctttttt gcggtgaagc aataacaaat tgttcattca
     1501 atttttcttc gtattctttt gcatgcgtaa atgtcacatc ttcgcctaag aaacgatgac
     1561 ctcttgaagc tctgtctgtt ttgacatcca agatctcaaa cgggattatt tcgtcatcta
     1621 acaatgccac gatccagtga atagggcgaa tatattcaaa atcgtattct gcccagtgca
     1681 tcgttactgg gaaagtgagg cttttaatga catctgctaa acctgtcaat acttcttttg
     1741 cttctttgcc gtgagtgtat ttcgttacat agacatattc cacgcctttt aattctctaa
     1801 aagtgatagc atctgtcgtc agaccttgtc cgcggacaaa tccttcagct gctttcgtcc
     1861 agtttccatc tgcgtccaaa gcaatttttt tggctggtcc tttgacttct tcttcgctgt
     1921 ctgcttgacg ttctggaatg tttgttactt tgattgctaa gcggcgcggt gtagaaaacg
     1981 tttcgatcgt atcataagtc aattggtttt catctaaaaa ttttgctgtt ttttcttcta
     2041 attgtttcat gcttggtgtg acaacatgag caggcatttc ttccagcccg atttcaagta
     2101 ataagtcttt tgccatctta ttcagcctcc ttcatgtttt ctggatttgc tgtgttttta
     2161 ttcaataaag ggaatcctaa tttttcgcgt tctgccacaa agattttggc tacagcacga
     2221 gccatgttgc ggatacggga aagataacct gctcgttctg ttacggatac tgcaccgcga
     2281 gcatctaata aattgaatgt atggctgcat ttcaaaatat aatcatacgc tggatgaaca
     2341 agattttcat cgatacagcg tttagcttct ttttcaaaac ggtcaaagtt gcttagtagc
     2401 aagtcttggt cacttacttc aaaagaatat ttggaatgtt catattctgg ttgtttgaag
     2461 atctcgccat atttcacacc atcagcccat tccagatcat agacactttc aacttcttga
     2521 atatatgaag caagacgttc tagtccataa gtgatctcag aagtaactgg tttacaaggt
     2581 agtcctccga cttgctggaa gtacgtgaat tgtgtgatct ccatcccatc aagccatact
     2641 tcccaaccca caccggcaca ccccatcgat gggttctccc agttgtcttc aacaaaacga
     2701 atatcgtgtt ctaatggatc aattcctaaa agacgcaagc tttctaaata aagctcttgg
     2761 atattttcag gtgaaggttt cattacgact tgaaattgat gatgctgata caaacggttt
     2821 gggttttctc cataacgccc gtcagctggt cggcgggatg gctctacata agcagcattc
     2881 catggttccg gtccgattgc ccgtaagaat gtatatgggc tcatcgtacc tgctcctttt
     2941 tctgtgtcat aagcttgcat cagcatgcag ccgtttgatg accaaaattt ttgtaatgtt
     3001 aaaatcatat cttggacggt caatttttta ctcatctgtt ttgccccttt caatttttaa
     3061 gcgttgctgt caataagaat acaggaaaga aaacgacaaa aagtccctat gtctgattac
     3121 agacataggg acgaataatt tcgcggttcc accctacttc cgaataaaga ttcggcagct
     3181 tcatttatcg tactcgcaag tgcctcttca aaaatcgttt gggtccggct tccactctcc
     3241 cgaactcgct aaaccacgtg ttttttactg atcttggtca atgtacgtat tcattttgca
     3301 gttttcttgt ttatataata gatcctctca gtgaacgatc tatttaatct acggttaata
     3361 atagacggaa gtccctgtat ttgtcaaata gttttgattt ttttgttgta tctttcattt
     3421 attcttcttt ttcaggtatc tctttttcag gcatattttt ctctggcgtc tttaatacgt
     3481 gtgcccaaga cttcatttga tcaatgaact ttttgctttt tagatgaata ccgacatatt
     3541 catcatacaa ctggtcaatg aggtcacgga ttgcctgctt cgtttcttct ttcaagtgga
     3601 ttccactgat cttatcataa gaaatagccg aaaacattcg aacgaaataa acagcgcgag
     3661 gatctgcatg ataacggtgt tcgtccattg gccagtgttt ctcgcatatc acaccgccag
     3721 atttggaaga atagtcaaat tttccttttg tttccccgca tacgacacag tgcgtccaaa
     3781 ttggcgcgat tccgaagcgt tgcagtattt gaacttcaaa gatattcgtg atgatttctg
     3841 catcatctcc ttgatccatc cgctgcagag cttgttgcgt aaattggaat aaatgcgggt
     3901 catattgatg atcttcgatt gctgcatcta ctagatttag caaataggta ccataaccgc
     3961 tcaaaaagat atcttcttgg atgttccgaa acgcatggac gtctttgaca ctgcttaaaa
     4021 acgacaaacc ttcacttcga aaattaccga tatagactgc ttcggtaaag gggagcaaag
     4081 ccggagctaa tggctgattt tgccgatgga ttcctttgat ataaaacatc attttgccag
     4141 aagattccgt aaagattttg actaatttat ctttttcttt gtaattacgg ctagataaaa
     4201 tcaagccttt cgattctgca agctggctca tattctccct cctaaaaaat aaatctggaa
     4261 aagcagattc tgccgatcca gattatttat gtttcgatct tccgccgttt ttctcaacgt
     4321 gcggattttt atccagttta tttctatact taacctataa gaagttcaga atatcagtat
     4381 tcgtcttttc ggtagccaaa gtcttgcaga tagacttttt tgtctcgcca gtctttttgg
     4441 actttgaccc aaagttctag aaatactttg tctcctaaaa gattttcgat atcctttctt
     4501 gctttcgttc ctacttgctt cagcattttt ccgcctttgc cgataatgat tcctttttgg
     4561 ctatctcttt cgacgatgat cgtggcctgg atatggattt tatcattctc gtttcgcttc
     4621 atcgaatcaa caacgactgc gacagaatga ggcacttcgt ctcgtgttaa aaacaagact
     4681 ttctcacgga tcaattcaga aacgataaag tattcaggat gatcagtcac ttgatcatct
     4741 ggaaaatatt gtgggccctc tggcatttca ctgactaata catccattaa gcgttcgaca
     4801 ttgtttcctt ctgtagcaga gatcggaatc acttgtgcaa aaggcatctg cgtcgaataa
     4861 tcttcgatga ttcccagcaa ctcgtctgga tggatcttat cgattttatt gataatcaga
     4921 taaactggag aatgcacgtt ttttagacgt tcaataataa aatcgtctcc ccggccgcgt
     4981 ttttgatcag cactgatcat aaataagact gcatccactt ctttcaacgc actgtaagct
     5041 gcttctacca taaaatctcc tagacgatgc tttggtttat ggattcctgg tgtatcgata
     5101 aaaatcagct gagcttctgg tgtagtatat actccttgga ttttatttct agttgtctgt
     5161 gccttgtcac tcataatggc aattttttga cccacgattc ggttcaataa tgtggatttt
     5221 cctacattcg ggcggccgac gattgcgaca aaccctgatt tatgttctgg catatacgtt
     5281 ctccttttta attaaacaac aaatgatata ttttagggac aaataaaatc aacccaatga
     5341 tgacagcaaa acaagcggtc actaaaactg ctccagctgc catatctttg atttttttcc
     5401 caatcggatg aaaatgaaaa tccgtaaaca tgtccactac attttcaaaa acagtattga
     5461 tgatctctac tatccaaacg agaaaaacag ctaaaagcag ccataaccac tctatccgat
     5521 caagctggaa aatcacgccc ataaataaag caaggatacc gaacgcaaca tgtttcctca
     5581 tattgcgttc ttcctcaaaa acggttttga ctccttggac agcaaattcc acagacatca
     5641 ggaaatgttt gtttttctct acgtttttat cttttgagtc cataggcatc caagatctct
     5701 ttctgtaaac caaacatttc tttctcatct tctggtgtca tatggtcata cccgttgata
     5761 tgcaagaatc catgtaccgc taaaaagcca agttcccgat catatgaatg gccgtaagct
     5821 tctgcttgtt cagcagcacg ttcaatagaa atcatgatgt ctcctagtac acgaggcaaa
     5881 ggattctctc catcttcaaa aatcactggg atttcgtctt cgccttcttc ttccagagca
     5941 aaactgatga cgtctgtcgg tgcatctttt ccacggtaat cacgattgat tacttggatt
     6001 gccgcattgt ccatgaaagt aacagacatt tctgtttcct ctggcagctt taaataagat
     6061 gctgcaaatt gcagcaagtc atcgatttct ttcattttct cttctgagac ttggtttgtt
     6121 tcatccataa aggtaatatc cattatacat cgcttctttc tattcttctt gttcttcctt
     6181 tatcttctca acttctgttt tcattttagg atatttgatc cgagaatgga aggtgctact
     6241 taaaccgctg atcaatgatt tttccacttt tcggatttct ttcaaggtca gtccactatc
     6301 gtctaattga ccatcggaaa tacgctcttc gataagatta tggacaaaac tttcgatttt
     6361 ttcgctcgtc ggatgatcca ttgcccgtac tgcagcctca caactgtcag caatattgac
     6421 gatccctgct tcacgacttt gcggttttgg tccaggataa cggaattgct cttctgttac
     6481 ttccggattg cgttctttcg ctttgatgta gaaataacgc attagcgtgg ttccatgatg
     6541 ctgtttgcaa atatcgatca ccatttgcgg catattataa tcttccaata ttttggctcc
     6601 atctgtcaca tgtccaaaga taatatgttt actgtcttct ggcaataaga aattgtgcgg
     6661 gttttccgct cctgaaggca agttctcaac gaaaaagcta gcatgtttga tcttgccgat
     6721 atcatgataa tagcaagcta ctcgagttag caaagaacgt ccgccgattt ctgctacagc
     6781 gttagcactt agattagcta ccatcatact atgatgataa gtacctggcg cttcttccag
     6841 caactgtttc aataaaggat gatttggatt gctgagttca tttaatacaa ttacactgtc
     6901 atcattgacc atcaattcaa tatatgggtg tagaccgatc gtcagcaaga atgagaagag
     6961 tgcaccagtg aacccgcaga caagcattaa ccaagtcgtt gcatcaccaa agctcattcc
     7021 ttgatagacg atcaaaatga catccatcat cacaggaaaa ataatgaccc acatggcagc
     7081 agataatcct tgttctgaga tccgtttgcg tctgacaatc gttgccatga gcccagagaa
     7141 aacataagac atcaagataa tcgttagaaa gttcgtgcca atcgaatcat aaaaaatgaa
     7201 aagtgcagat actgcttgga aaataccagc catgatccct gctcttcggt tcaagaaaaa
     7261 gcttaaaaca agtggtgcaa aagcagcggg ataaaacagc gggatataag tagcttgttc
     7321 cgtttggaat aattggaaaa atttcatcag cactatgctg actgacatag ctgtcacata
     7381 gaaaagcacg tattttgttc tttctcctgc attttggaat tgaagtgaat tataaatcag
     7441 tacgccgatt tgaagcaaaa cagctaaaac catggcaatg agtgggaaaa tcgaagtcgt
     7501 ttgattcgtc aatcctagta attccaattt tttcatcgct gaagcatcaa tttgatttcc
     7561 ttcgcgaaca atgatttctc cttggaagat catcactggt tgtacagctt ctcgtgcaga
     7621 ttgttttaat tcttctgtct ttttctcatt caatacatca ttgactacaa tgccttgatc
     7681 cactaagtag cgaatcattt gttgctgctc tgttgtgaca ttcaaatact gaatctgatc
     7741 ttctgcttct tgtttaaaag cttccaattc gctttcacga acttgtttac tcatctgttc
     7801 gtcaatgagc ttcaaacttt catccctaac cgtgtgaatt tccgtttcag tcatttcaaa
     7861 tatcgtcgaa taaaaattgt tcggcagttt ttggtaaaac gcaacgtttt ctgcattcac
     7921 gttttcaaaa tttttcttca gtgtagcaat ccgctcttct gtagtaggtt tcgggatatt
     7981 ttcatcttcc ttcgccttat ctaccttgtc attataagat ttgttgatgg tatcattcgt
     8041 cttatcgatc aattcgaata attgcttgat ccgattgttc tggtcatctg ccagatcttt
     8101 ttgataagta tattctggag tcactgcttc tgctgccagt tttcttcttt gttcggtttc
     8161 ttccgtgttc tcgatcgttt tattcgcacg gatactctct tctgctactt ggccttctct
     8221 gaaatcattg gccttttgtt tgacactact aaatgtaacg ccgcagacaa tgataaaaaa
     8281 caagaacaga aggaaaggca atgctgcctt tccgagtttt tcttgtagtt tctgcagtgt
     8341 tttattcaac atgtgcttat cctcctaagc aaagcttatt cttgatgaag atccgccttt
     8401 tcataagctt tgatgatttc ggcaactaca gggtgccgaa caacgtcact tgcttcaaaa
     8461 ttaacaaaat cgattttttt gatttgcttt agtgttcgtt ctgcatgaac aagaccactt
     8521 gtcgtccctc gaggcaagtc tatctgactt gtatccccat tgacaatcat ttttgattga
     8581 taaccgagac gggtcaagaa cattttcatc tgagcgactg tcgtgttttg tgcttcgtcc
     8641 aagataacga atgcatcatc caatgtccgc ccccgcatgt aagctaatgg tgcgatttcg
     8701 attacgcctc gttccatcaa tcggttcgta tgatccaatc caaaaatttg gtataaagca
     8761 tcataaacag gccgcagata aggatccact ttttctttca gatccccggg tagaaagcct
     8821 aggttttccc ctgcctctac agcgggccgc gtcaaaatga ttttttgcac ttcgcctttt
     8881 tttagggcag caattgctaa tactacggca agaaacgttt tccctgttcc ggctggccct
     8941 acaccaaata cgatgtcatg tttggctacc gattcaacgt atttcttttg accgctgttt
     9001 tttacccgta taggttttcc attacggtct ttgacgatct cttcctcata catctcaaca
     9061 aaataatcta atgtaccttt ttgccccatt ttcaatgccg tcacgacatc tggtgtactt
     9121 acgtggattc ctcgtttgat caattcctgt aatgtccgaa tgactgaggc agccaaggaa
     9181 atctgggatt gttcaccaat gatctgaatg atttctccac gtgtatggat ggttgtttcc
     9241 gttgtttcct cgatcagttt tatatgcttg tcgtgtgcac ctaaaagcat gctgacatca
     9301 tcagcagctg tcaatctaat ctctaaagaa ctagactctt ccgtcaaaaa tagacatccc
     9361 ctttttttcg attcattctt tcctatttta ccatactcag aagaaactat atagcaaata
     9421 agaaaaggtt gtaaaagagt cgtttaacca tacgtaccaa gaaacgttaa aaaatagctg
     9481 tttccattcg ctaaaggtct cgttttgata aaaagaatct gagaatgtaa gttatatgaa
     9541 agtggagaac aaaatattcc gtttcattca gaggtattct ctaggtattt ttcttgtttc
     9601 aaaaaaagaa aacaaaatag ttaaaatatt cttgcatttc aaaaaaaaca ggtgtattat
     9661 tacgtcaaca aataaacata cagcaaagcg tcgaggaaaa cagtaggcta ttcaacgatt
     9721 tattcccagt gagtcatgta agtgagaaat gacaataaat cgaatagacg aacatcattt
     9781 ccgagtcaat gtcgaaaacg tagatcattg tggctgcctc cattatcagg ctagcggata
     9841 actttatcaa aaagcgtccg tgaatgaaga agctttttta agcgaaaaaa ggtggtaccg
     9901 ttctagaatc tagagccctt ttcccaagtg gaaaagggct tttttttata ttttaaggtg
     9961 gtggttttca tggaatctga tattagtggc attactaatt gctgcaacgt ttatatcgtc
    10021 aatcaaaatg ataattattg gaggaaaaat ttatggaaga aacaaaaact tattcatgga
    10081 aacaactatt gatggtcagt tcacttatct tcgggatgtt tttcgggtca ggtaatctaa
    10141 tctttcctgt ccacttaggt caaatggctg gcgcaaactg ggtcagtgct ggcgtaggat
    10201 ttgccatctc aggcgctctt ttcccattac tagctatctt agctgtagta gtgacaaaaa
    10261 gtgatggtct gtatgactta gctaaacctg tcggaaaatg gtatgctgcc ttgttcttaa
    10321 tccttgttca tttgacgatc ggaccatttt tcggtacacc acggacagct gctactgctt
    10381 ttgaaatggc tgctaaacca tttttgcctg aaaaatataa ttcattaggt atgtttttat
    10441 tctctgctct tttcttttta cttgcttatt tccttactgt ccatcccaca cggttggtca
    10501 aatacgtcgg aaagtatcta aactccttgt ttcttgcatt attagcaatc atttttgtcg
    10561 tttctttatt ccacccaatg ggaaatctaa atcaatctgc tgcagatact taccaaacaa
    10621 acgctttaac aaatggtgtc ctagaaggtt ataacacggt tgatgcagta gccttgcttg
    10681 ccttaagtgt cacttttgtc catgcagtcc gtgaccttgg tttcaaagat aaaaaagtca
    10741 cagaattaac agcaaaagct ggtagcctga gcgttgcgct agaagtcatg atctattttg
    10801 gtcttgttct tctcggtgcg atgagtttga ataagatgtc tttatcagaa aatggcggaa
    10861 ctgcactttc tcagatcact aatttttatt taggtaactt cggcgctagc ttcttaggag
    10921 tgatggtcac attaggtgtc ttcactaccg ctatgggact cgttgtttct tttgcacaag
    10981 atttccataa actgttccct aaagtcagtt atatgacatg gctgcggtta acgactttcg
    11041 tctcttttgt cgttgcaaat gctggtttgg acaatatcat ccaatggtct ttaccagtat
    11101 tgatgctttt gtatccattg tcgcttgcgt tgattttgct ttccttaaca gcaaaatttt
    11161 tccaaaagac accatttgtc tatcaagtaa cgatgttgtt tgctgctgtt cccgccgtat
    11221 tggatatgct ggcaaattcg cctgctttag tgagtcaaca aagagttgta gcaagcatgc
    11281 tcgaatttta tcatcatcac gttccttttg ctgcattggg acttggctgg atggtcccaa
    11341 cgttacttgg gtatgctggc agcttactct tctattacgc ctatcgccta tcagggtata
    11401 aacaagaagc aaatgaacta ccagaagaat aaccagatca aacacaattt aacataagaa
    11461 atattaaaca ggaaaaaaac agctagatga aagaatccta ttctttcgtc tagctatttt
    11521 ttagatctct tttttacgag cacaaaaaaa ctgcggacaa tgattgtccg cagtttaaat
    11581 gataaaatta agattgtaat agttctttca caatggcatt tacttggttt ccatctgctt
    11641 ttcctttgac tttaggcatg atcgcaccca tgactttgcc aaattctttc attgatgaag
    11701 caccagtttg cgtgattgct tcttgaacaa gttgtcgaat ttcttcgtcg gaaagttgtt
    11761 ccggcatgta tttttcgact atcacaattt cactttttac tttttcagct aaatcgtcac
    11821 gtcctgcttc ttcaaactca tgcaaagaat cacgtcgctg tttcatttcg cgagacaaca
    11881 ccgttaattc ttcttcttct gtcaagtcgt gaccttcttt aatctgctcg ttttgtatag
    11941 atgacttgat cattcggatg acttgtaaag attccttatc tttcgctttc atcgctgttt
    12001 tcatgtcatc gtttaatcta cttagtagtg acacactacc aactccaatt catcacagaa
    12061 ctagaatttt ttgcgttttc tagctgcttc agattttttc ttgcgtctta cacttggttt
    12121 ttcgtagaat tcacgtttac gagattcttg taaagtaccc gcttttgaca cggaacgttt
    12181 gaagcgacga agagcatcgt caagtgattc gtttttgcga acgactgttt ttgacatgtt
    12241 aattccctcc ctccgagctt aaaaagtaac cgtaattgtt attgaaatat atctcaaaaa
    12301 acaagactgt cctgtaaata ttatatctaa tgctgttttg accgtcaagg aaatcttgct
    12361 tttttttaaa attatcaaat gttattttat ttaaatcctt attgacaatt ttcacatctt
    12421 ccgaaaatct cgaatctatg cccttcgatc gtacatccac cgagctgttc ttcgaaataa
    12481 ttcattggac acatatggat ttctttcgtt gttccgcaca ccgtacagat aaagtgatgg
    12541 tgatggccga tttccccttg acagcaatga aaacgaaact tcatctctcc atttaaatcg
    12601 gtttcttcca gtaaatcgat ttctgcaaaa tcatggagat ttcgataaat cgtatcgtaa
    12661 cttactcctt tgaattcctg attcatatag tcaaaaactt ctttagcgga tacgtagcga
    12721 ttgcgcttca ctaaataagt taacaatgct tctctttttt tagtatattt aaaaccgtta
    12781 tttttcaatg tttctagtga ttgttcgatc aacgtctgtt ttttcatgac ttattcctcc
    12841 aaatcaaaat gattacgaag tacacattct agagtatggt ataataaaca taaaaaaaat
    12901 gcaagtgagg ttttatccat gtcttccgat tctacagaaa tcgttacttt ttttgtcatc
    12961 tccgactctg ctggagaaac tgcgacaaaa ctagcgcaag ccactatggc ccaatatcct
    13021 agcgttgagt tcaatttatt ccgcagaaca tttgtgacag atgagaaaac attgatgcag
    13081 gcactgcaag atgcactaga agaaaaagct cttgtcctgc acacattgat caatcaagaa
    13141 ttgatcgaga tgacgcgttc tttttgtgaa gaacatcaat tatttagttt tgatgtgatg
    13201 actccgccga tatcagaaat cgagcgctta actggtatca agcccattcg gcaacctggc
    13261 gcccttcatt tattgaatga gaactatttc aaacggatca aagcaatgga atttgctgta
    13321 aaatacgatg acgggaaaga tcctcgtggg tttctagaag cagatgtcgt tttgctaggc
    13381 gtatcccgaa cttccaaaac gcctttgagc ttgtttttag ctaacaaaaa tttaaaagtt
    13441 gccaatctac ctttgatccc acaagcacat attcccaaac agctttggga gatcgatcct
    13501 aaaaaaatcg tgggactgac gaataaccca gatattttaa acaatatccg taaagaacgg
    13561 atgcgctctt acggattaaa tcctgatact gcttattccg atatcgaaaa aatacgagct
    13621 gaactcgatt ttgcgaatga tctttatgaa aaattgggct gcgtagtaat caatgtcgct
    13681 tctttatcta ttgaagaaac agcttcgctt attttgaatg ctttagacct agaagaccat
    13741 agttattatg gaactgaaac caccgaagaa acagactaac aatttttctt taaaaaaact
    13801 ggactgtgtc agcgaagaag attcgttgac acagtccagt ttgtctctat ttgcacctat
    13861 cgttattgat gttccacatg tttgagtgtc tggttcaaaa tatccagtac gtgagaatca
    13921 tcaagactat acaaaatcgc ttttccctca cgatgggact tgacgacatg gttttctctt
    13981 aataaacgca gttgatgcga tacagcagat tgttccattt ccactgcttg gatgattgct
    14041 gtcacatttt gttccccgtc tttcagatac agcagaattt tgagacgagt ggggtcactc
    14101 aacattttga atagttggct gactttctct acttttgttt tttgcgtgtc tggcaatcca
    14161 ttcacctcca acttgaaaaa agactgactt ctaaagttta ccactttttc catcagtctc
    14221 aagttttttt attcttctgt catcttttgg ataatcgttt cataagcggc tactttttca
    14281 gctgcgtctt ctgaagagtt tccttttaca ccgatataaa atttgatttt cggctcagtt
    14341 cctgaaggac ggatagcgat ccagccattt tcttccagcg tatacttcaa tacgtcagat
    14401 ggcggtgtcg ttagtttttc tacgtttcct tcacggtctg ttctcgtcaa ctctttgaag
    14461 tcttctgttt ggatgacttt tgctccggca aattctgttg gtgcttcttc acggaatttt
    14521 tccatcaaag cagagatttt cgcagaacct tcttgtccgc tcattgtcac agaaatcgtt
    14581 ttttctgcgt agtagccata ggtttcaaaa atcgtttgca agccatcata caacgttttg
    14641 ccttgttttt tatagtaagc tgctacttct gctaataaga gcaatgcttg gatcgcgtct
    14701 ttgtcgcgaa cgaaaggttt aactaaataa ccgtaacttt cttcgaatcc gaacataaaa
    14761 gtatgggaat gatcttcttc aaattgttga attttttcag caatgaattt gaatcctgtc
    14821 aatacattga acatggtagc cccatagtgt tccgcaattg ctgtaggtaa ttcgctagaa
    14881 acgatcgatt tcaataccgc tgcgttttca ggtaaagtgc ctcgcgattg gtgtgcttcc
    14941 aaaatatatt gagcaaacag tgctccgatt tggtttccgg tcaatacttc ataggagcca
    15001 tttggcaaac gaacggctgc acctaaacgg tctgcatcag gatctgttgc aatcagcaaa
    15061 tcagcgcctt ctttctcgcc aagtcgtatc gcatactcaa aagcagagtg ttcttctgga
    15121 tttggtgatt tgactgtagt gaaatctggg tctgcgacag cttgttctgg aactagtacg
    15181 aattgttcga atccagcttg tttcaacgca cgttcgccca acatttttcc tgttccgtga
    15241 agtggtgtgt acacaagttt caaatctttc cccattgttt ccaccaattc atgatcaatc
    15301 gttactgctt tgatttcttc tagataagcg gcatccactt cttctccgat gatcgtgatc
    15361 aatccgctat gttcagcttc ttcgtcagat aatacttcaa cttttaacgg attgctgact
    15421 tcacggacat atttcgtcaa tgcatctgcg tctgcaggag gcatttgtcc cccatcttct
    15481 ccgtagactt tatatccgtt gtaagctgct ggattgtggg atgcggttac catgattccc
    15541 gcaaacgtct tcaaatgacg gactgcaaaa gaaagctctg gtgttggacg tagactttcg
    15601 aatacataag atgggatatt gtgatgtgct aatgttttgg ctgcttccat ggcaaattct
    15661 ggtgattgat ggcgtgagtc ataagcgata gcgacacctc ggcgttttgt ttcttcgcct
    15721 tgtgtatcca tgaaacgtgc aagtccttct gttgcttgtc ttaccgtata gatgttcatt
    15781 cgattgatcc caggccctaa gattccccgc atgcctgcag taccgaattc caacggtgca
    15841 taaaaagcat cttcaagttt ttcaggatct tcacccagat catttaattg tttttttaag
    15901 ttttcttcta aattttcttc atgtacccat tgttcataca cttgttccca agacatgaaa
    15961 ataacacctc tttctgaaat atggttctta ataagtatag cattcactta ttagatatga
    16021 aagtatgagg accaaaaaac gcttaaatca agaaaggatt cttttatcaa gcacggttgc
    16081 aagtattcgc ataaatcgga ttcagtagat ttcacgcgaa aaaaaagagt aaaagaacaa
    16141 ttaaaccgtt cttccactct ttttaaactt atgcagttgt ttcttctaaa gattccaagt
    16201 atttatacac ttgttgtcca gcgatcccac catcaccaac agcagtagtg atttgtcgca
    16261 aatctttttc acgaacatct ccgatggcaa aaacgcctgg tatattcgtc ttcatttcgt
    16321 tgtctgttgg aatccagcca gcttcgttcg tcaaccctgc ttctctaaat ggtgcagtta
    16381 atggatccag tccaacatag atgaatactc cgtctgctgc caaatcaaac tcattgccgg
    16441 ttttgacatt acgtgctttc acacctgtca ctaccatttc attcccaacg atttcgtcaa
    16501 cgactgtatc ccacataaag gaaatttttt cattcgcaaa tgcacggtct tggatgatct
    16561 tttgtgcacg taattcatct ctacggtgga cgatgactac ttcagaagca aattgtgtca
    16621 aatagatggc ttcttctact gctgagtctc ctcctccgat cactactaga cgtttgttac
    16681 ggaaaaacgc accatcacaa actgcacagt aagaaacgcc acgtccagca aattcttcct
    16741 cgccaggtac gcctaattta cggtggatac agcctgttgc gataatgact gtttttcctt
    16801 cgtaagtttt atcttcacaa ataacttctt tgtggctgcc gtgatccttg atatcttgca
    16861 cgattccata agcattttct gtcccaaatc ttgaaacacc ttcatacatc ttataagcta
    16921 gatcaggacc cattattgaa tcgaaaccag gatagttttc gatttctgcc gtattgttca
    16981 tttggccgcc aggggcgcct tgttcgatca tcaatacgga taagtttgaa cgtgaagcat
    17041 ataaagcagc agtcatccca gcaggacctg cgccaataac gattacatca tacatatgta
    17101 gattctcctt tacttaatca agtaagcata ctttacagcc aatgcgaaaa aaagtctatt
    17161 gttctgcttg cgtttctcga atagctttat tatactcgaa aaatccgctg aaaaacaata
    17221 gacttttata aatcattgat caatcattcg aaagattttc tccatttgaa tcaattactt
    17281 ttttatacca gttgaatgat ttcttcttcg aacgggctaa tgtgccattt ccttcgttgt
    17341 cacgatcgac ataaatgaat ccgtaccttt tcttcatctc acctgtacca gctgatacta
    17401 aatcaataca tccccaagtc gtatagccta atagatcgac tccatctaat tctactgcat
    17461 ctttcattgc ctggatatgt gctgccagat agtcgattcg atagtcatct tccacataac
    17521 cattctcatc gggcgtatcg actgcaccta agccgttttc tacaataaat agtggttttt
    17581 gataacgatc ataaatatca ttcatcgtta ttctcaagcc taatggatca atttgccaac
    17641 cccattcact tgcttccaga tatggatttt tgacagaagc aaagatattt cctgcagtct
    17701 gttccaagag ttcgttgtca gtagtcgaaa ctcgagaaga ataataagaa aaggagataa
    17761 aatcaacagt atgttctttc agcaatgcgg catccccagg ttccattttg atctgaatgc
    17821 catttcgttc catttctttc aatgcataag caggatattc tcctcgtgac tggacatcaa
    17881 tgaagaaata attttctcga tctgattttc tagaagccca aacatcttca ggattgcaag
    17941 tgtatggata atatgctcct gctgccagca tacagcctac tttgttctcc ggatctactt
    18001 catgagcaat cttcgttgcg atggcacttg caaccaactc atgatgtgcc gcttgatatt
    18061 tgatttgttc ttcattttct ccttcttcaa aatacagccc tgcgcccatg aatggtgcat
    18121 gaaggatcat attgatctca ttgaatgtca accaatattt gacaagtcct ttatagcgat
    18181 tgaagatgac tcggcacaga ttttcataaa aaccaacgag ttctctggaa cgccacgcac
    18241 catattcttt gatcaaatgc atcggacaat caaaatgtgt aatcgtcaca agcggctcga
    18301 tcccgtactt ttgacattct ttaaagacat cctcataaaa tttcaaacca gattcatttg
    18361 gctctttttc atcgcccatt ggaaaaatac gactccaagc aatggacatc cgataagtct
    18421 tgaaccccat ttctgcaaat aaagcaatat cttccttata atgatgatac atatcgatcg
    18481 caatttttgc tggataaaaa tgattttcat caaattcaaa cattttcttt tttccagtaa
    18541 tgacaggaaa acggtcttct ccgattggca caacatctac gttcgctaac ccacgtccgc
    18601 cttcatcata accgccctcg cattgattgg ctgctgtagc tccgccccat aaaaaatctt
    18661 ttctgaatcc catcatactt cctccattac atgtgtattg cttgataatt ctcttgccag
    18721 ttcatcttgg acttcgattc cttctgcttt taaccggctg attgtttcct tgaattgcgg
    18781 taaatactgc tgatgtgcaa taaacaactc attcatgatc cgttttgctg tctttcctga
    18841 tcgtataagc ggattgatcg taaacgcttg taaagctgtt ccataatcac cagtcacagc
    18901 tgcttcgatc gtcagcaatt ccatattctt cattacttgc aaccagcctc tttcagcagc
    18961 aggaagggaa ccaaacgcta catttcgtgc gccttgtgcg ccaacatagg cagtgacttc
    19021 tactacacaa tctgccggca gatcaggaac agctccatca ttctttgtag ataccacgat
    19081 ctgtgtttct ttatttccat agatcgaagc aatcgtttca caggcagcat ctgaataata
    19141 agcaccacct cgtttttgca attgttcagg tttatgatcc aattctggat ctttgtataa
    19201 ttcaaacagc tctgcttctg tttctttcac ttgctgcgct cttgtaccaa tcgtttgata
    19261 ttcttctaaa gcatgttcca gcatttcttc ttcacggtag taatagcggt ggtagccaca
    19321 agggatcatg ttcatttgtt ttaattgttc tttgaaaaac gggatatcaa agatattttt
    19381 cggcaatcca ttgtcttcat cgtataaacg atcaatcaat tctgccgtgc ggtcatttcc
    19441 tttattatct gctactttgt gccaatggaa gtgatttaga ccggcaaact tgtagatcag
    19501 ctcttctttt ttcttgccaa gcattgttgg ttctgtcatc atcgccatta ctggcacatt
    19561 gcataatcct atcactttat cccatttgcc atatctgata actgcttctg tcaccatccc
    19621 gcttgggttc gcgaaattga tcagccaagc atttggacat aaacgcttca tgtcttcaac
    19681 gattgccaaa accactggaa cggtacgaaa agctttgaat atcccgccag caccatttgt
    19741 ttcttgccca atcatcccat aataactagg gatccgctcg tctttgattc gtgcatctaa
    19801 ttgaccgaca cgaaactgag tcgtaacaaa atccgcatct ttcaatgctt cttcccgatc
    19861 cagagttgca tagattttga cttcatacgg agaagcatcc cacatacgct gagacatctg
    19921 actgacgatc tcaagctttt cttttccttc ttctacatct acgagccaga tttctcgtat
    19981 cggtaattcc tcatatcttt tgataaatcc ttccatcaac tcgggggtgt aactgctgcc
    20041 tccaccaatc gtagcgattt tgattccttt tgccatgttc tcactccttt actttttaca
    20101 cttttattat cttttttgta aacaaaacaa acaatagaaa ttcgtttctt tccagtaaca
    20161 tttacaaaag ttagtgtttt ctactggatt tttgtaaata tcttgtcaaa aaa
//
LOCUS       DANKHL010000032        20091 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000032 DANKHL010000000
VERSION     DANKHL010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.034"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(125..895)
                     /locus_tag="PW101_000807"
     CDS             complement(125..895)
                     /locus_tag="PW101_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2513231.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(888..1823)
                     /locus_tag="PW101_000808"
     CDS             complement(888..1823)
                     /locus_tag="PW101_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2513232.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(2417..4066)
                     /locus_tag="PW101_000809"
     CDS             complement(2417..4066)
                     /locus_tag="PW101_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL2513233.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(4261..5157)
                     /locus_tag="PW101_000810"
     CDS             complement(4261..5157)
                     /locus_tag="PW101_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2513234.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(5150..5830)
                     /locus_tag="PW101_000811"
     CDS             complement(5150..5830)
                     /locus_tag="PW101_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2513235.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(5831..6613)
                     /locus_tag="PW101_000812"
     CDS             complement(5831..6613)
                     /locus_tag="PW101_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2513236.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(6606..7523)
                     /locus_tag="PW101_000813"
     CDS             complement(6606..7523)
                     /locus_tag="PW101_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2513237.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(7526..7810)
                     /locus_tag="PW101_000814"
     CDS             complement(7526..7810)
                     /locus_tag="PW101_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513238.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            8598..11825
                     /gene="fss3"
                     /locus_tag="PW101_000815"
     CDS             8598..11825
                     /gene="fss3"
                     /locus_tag="PW101_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HDL2513239.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            11937..12251
                     /locus_tag="PW101_000816"
     CDS             11937..12251
                     /locus_tag="PW101_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2513240.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            12264..12638
                     /locus_tag="PW101_000817"
     CDS             12264..12638
                     /locus_tag="PW101_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2513241.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            12639..12992
                     /locus_tag="PW101_000818"
     CDS             12639..12992
                     /locus_tag="PW101_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HDL2513242.1"
                     /translation="MKNMEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQ
                     GLLEVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFL
                     KQLVETKQLPVVVND"
     gene            13061..14077
                     /locus_tag="PW101_000819"
     CDS             13061..14077
                     /locus_tag="PW101_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL2513243.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            14671..16545
                     /gene="ltrA"
                     /locus_tag="PW101_000820"
     CDS             14671..16545
                     /gene="ltrA"
                     /locus_tag="PW101_000820"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL2513244.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            16671..17027
                     /locus_tag="PW101_000821"
     CDS             16671..17027
                     /locus_tag="PW101_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HDL2513245.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            17072..18214
                     /gene="dcm"
                     /locus_tag="PW101_000822"
     CDS             17072..18214
                     /gene="dcm"
                     /locus_tag="PW101_000822"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HDL2513246.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            18204..18493
                     /locus_tag="PW101_000823"
                     /pseudo
     CDS             18204..18493
                     /locus_tag="PW101_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18749..19933
                     /locus_tag="PW101_000824"
     CDS             18749..19933
                     /locus_tag="PW101_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL2513247.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            19930..20043
                     /locus_tag="PW101_000825"
     CDS             19930..20043
                     /locus_tag="PW101_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDL2513248.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
BASE COUNT         6424 a         3834 c         4036 g         5797 t
ORIGIN      
        1 cactaaacgg tgttcaatca gctgaccctc ggtattcgct caaaaaacaa caaaatatga
       61 ggaactattt tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc
      121 ttttctaact tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa
      181 caacgatgaa taacacaagt acgatcatcg atgttgctac tggcacatcg atcgttccaa
      241 ggaatccgta acggaaaccg gagatcatat aaacgatcgg attgactttt gaaacagctt
      301 gccaaaaggg cggtaacata gagatggcat agaaaacgcc acctagataa gttaacggct
      361 gcaagacaaa tgtaggcacg atcgatacat catcataaga ctgagcaaaa atcccgttga
      421 gcagtcctgc taatgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc
      481 atgaatacac atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga
      541 gaatactgcg acccacacca ccaatcacaa agccccaaat aatcacatgc gtaggaacag
      601 gtgctaccaa gatttcttcg atatttttct gaaatttttg agagaagaag gacgaagaaa
      661 cattggcata cgaactagtg atagcagaca tcataatcaa accaggaacg atgaattcca
      721 tatatgaaaa accacccatg tcaccgatcc gcccaccgat catttttccg aaaataacaa
      781 aatacaagga tgtcgtgatc actggcggaa ccagtgtttg gacccatata cgcaaatagc
      841 ggttcgtttc cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc
      901 tcctccgtat gattattttc ttctgtgatt ttcaagaaca gttcttctaa acgattcgac
      961 ttattcctca tagaaagaac tttgattcct tgtcttgtaa gctgatcgaa gattccgttc
     1021 accccttgat ttcgttcaac ttcgacagcg attgtctgct catcttcaaa tgtacaagca
     1081 tatccttcaa tcacaggttt ggtatcatat ggcgctaaat caaagataaa tgtttcaaat
     1141 tgcagttttg acagaagctc tttcatactt gtattctcga tcagttcacc cgattggatg
     1201 attccgatgt tgcgacaaag catttcagct tcttctagat aatgcgtcgt caaaatgatc
     1261 gttgtaccat tttcgttcaa ttctcgtaga aactcccaca tctcgcgtct caactcgatg
     1321 tcaactcctg ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta
     1381 gcaatcatca gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt
     1441 tcccaaagat tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag
     1501 acaccgtagt agcctgcttg attcacgacg atttgctgga ccgtttcaaa agggttaaaa
     1561 ttgaactctt gtggaaccaa tccaatctgt tgctttgctc gaactagatc cttgtccaga
     1621 tcataaccga agacattcac ttttccagat gttttattga ctaaagaagt aataatcccg
     1681 atcgttgtag acttcccagc tccatttggc cctaatagcg catagaaatc tccttctttc
     1741 accgaaagat cgattccccg caatgcttcg accccacttc catagatctt tttcaaatta
     1801 tttatttcaa gcgcatttgc catttatttc ctatctcctt atagacatga ttcttaattg
     1861 tatcatggaa tacagcagat gaaaaaacaa cttggcttcc acacaaaaac acacctgcta
     1921 acaaagagga gatgtgtttt ctagttcatg atcatcttcg atagaataac tatctaaaca
     1981 actctggaac ttttaaaatc ataaaagtac cgacagcaac tactacgatg acatatagaa
     2041 gatccttata actgaggttc ttgtcttttt tattcatgtt aagctttcct ccgtcctttt
     2101 atcgaaagct tttttctttt attttaaccg aaataaataa aatggaaata gttattttca
     2161 atttctgttt tcgctgtttg ttttacggag aactttgcca tggaacgtgc gtctcgtcgc
     2221 tgatgctaag ttttttcgat aaaagctgct agaaggaaga cagcgtggtt tattttgtat
     2281 cgtcttttgc taaatcttcc aagatcatct ggacagcaga ctcgttccca ttgaattctg
     2341 ccactttttt ggcatcaatg tacttctttt gataatcgtc tttttggacc cacgcatcat
     2401 gcttcgtttg ataaagttaa tcaaacggat accactgacg ctgttcatcg aacaacaaac
     2461 tttctttttg ggcctgttca actatggatt taggataatt caaaactttc agcaaggcaa
     2521 tcgcatttcg ggtccttgaa ggtccttctt ttaatttgaa atcaaaactg attccttttc
     2581 cttctgttac ttgttcttca aagtggacat tctcacaata attctttaaa atctctgtca
     2641 attcgatatc atgtgtggcg acaaatgcta aagatggata ctccgcaagc caccgaacga
     2701 ctgaagcgga agaagcaatc cgttcgatcg tatttgttcc ttttaaaatc tcatctacga
     2761 agcagtagca gcgttctttg gaagccactt ggtccagcaa acgtttgatc gatttgattt
     2821 ctgaaacaaa ataactgtcg ccttcaaata aatcatcttc gattgccatg gaagtcaaca
     2881 catgtccagg ttgcagggta aactgttctg caatcgctgt ttggatcgtc tgtgctagaa
     2941 tacagctgat ggctatactt ttgacatacg tagattttcc tgatgcattg gaacccgtga
     3001 ctaatgtatt cttctgccag ttgactggat taactaccgc ttcttttaac aatggatgat
     3061 agatatcttt cgctgtcaca ccgccctcag taaactctgg tcggcaagtg atcggcatat
     3121 aggtgcgata attcaaaacg gctgctgcaa cttccaattc tcccagcaat tcccacagct
     3181 tgatggcttc agttgaatgc ttttctaatt tagtgagaac gaaatgatag gagataaatg
     3241 gcagcatgaa catgatgttg acatattcaa aaatcaattc tgcttcactc ccgctcttca
     3301 ttcgaaatga aaaacctaat ttagggatgt ctcttaatgg tttagtttgc tgtttcagct
     3361 ctttttgaag tggtgtcgtc atttttgcta actgattggc ggctgaaatc gttgaaacca
     3421 aataactcat gctattcagc tctgtttcta gtgtttgttt cttcactaga taatagaccg
     3481 tattgaatac taagccaccg attgctagat agattcctag tacttgccct aaaagaagca
     3541 aacctacacc caccagcgga agcaagccaa gaaaaataaa taaacctacg cgtccaatct
     3601 ttttactttt tccatccgct agatattgtt ttgcaaaatt atgatctttt tttcctaaac
     3661 gagcgaattg atattggatt ctttctctca gttgaggatt ttcctgataa aaagcaatta
     3721 gtttttctaa ctgataatct tctccaaaat caaagctacg taaacgctga tacaacgctt
     3781 ctgaacctac actagaatag gtactgttga tcccttcaaa tacttcgaac atatccaagt
     3841 cataccaagt aagatcgtcg atttcactat cccatgaacg gaattttttc tccgtgagcc
     3901 acgcatcctt caaactttct tctttatcaa atctcggttg ataaggaatt tttccccaat
     3961 tactacgaac tgtttccttt actttcaaac gtgaaaaaat gtctaaaaca actaataaaa
     4021 taacaactgc aataataatt aaaacgataa attctctttg gcccatatcc ctctccgttc
     4081 cttgatttgt tagttttagt atagcactgt tgcgctattt gaaaaggggg tacaaaaata
     4141 actgtcttcg ttacctaatt cgtatcaatc acaccaaaat aaaaacacct tccaaaaaag
     4201 aagatgtctt taaaacgaga tacaattttt tgtcattcac ttcaggtaaa ttagagccaa
     4261 ttaaaccctc cgcaacttga cggagatgga gaagatgccc ccgtctaatc tagcggtcat
     4321 ctctcctccc attccttcca ccaatttttt tgacaggtaa agcccgattc cgctagattc
     4381 cgtatttgtg cggctggtat tttcggtata aaaacgctgg aagacttttt caacagaggt
     4441 ttgcgctcct ggtttgatag gattggccac tgttaatcga atgaatgaat cctcttttga
     4501 aaatgtcacc tttgcttgtc catctgcata acgcaataaa ttctgtatga tattttgcag
     4561 cactcgcagc aacatgagcg gatctgctaa tacagttgta tttatcgagt gttcagcgaa
     4621 cgctacctcg atatttttct cagaaaattg ttcataaaaa gagaaaaata cttcttccac
     4681 ctctttagaa aaagaaagcg tatggagatc catttctttt tgcttcgtct ctattcgagc
     4741 aagatcataa aactgatccg tcatactgag taatcttgca acagattgtt tgatgatcgt
     4801 taaatacttc tcccgtttct ccgtatcctc tacctgttcc agtaattgta cataaccgtt
     4861 aatagcagtc aaaggagttc tgagatcatg agaaaggccg acgatcgata gtctgatgtt
     4921 ttcttccatt tcttgaaatg cttgtccttt ttgttcattt tcatccaccc atgtattcag
     4981 tgcatctact aggtccatta gattcttttc tcgaaattcg actgaaagac gaccaccata
     5041 ttcttgtcgt tcagaaagag tggcgatttt cttagttaat ctcttgattt ccctttgcag
     5101 gaagaaaata aaacttccca taaagataac aacgagaagc aacagccact taatcaaatc
     5161 taaaccccac tccccacacc gtatgaatat acacagcatg tgcctttgct agtttccctc
     5221 gaagattgga aatatgcata ttcactgttt tctcactatc aaaatatggt tcttgccaaa
     5281 ctgtttcata aagatttgct cgcgaaaata cttttttcgg atgttctaaa aaaagcttca
     5341 gcagatcgaa ttctcttcct gtcaaatgga cagccacacc attcacattt acatccctcg
     5401 actctttgtc cagtaagatt tccttgtaat agatgcgttg cgacagatct tttacttgga
     5461 cagaagtctg atgacgaagt tggatgccga ttcgtaacaa cagttcttcg atatcaaatg
     5521 gtttcgtcac atagtcatcc gctcctgctg ccaacaagga aactttatct ctttgatcgc
     5581 tttttgctga aagaacgatg atcggaacat tcgatgtctg tcggatttct gctaacaatg
     5641 cttcacctga aagccctgga agcatgagat ctagaaggat caaatcgaca ggatatgtgc
     5701 ttaacaataa ttttccttcc gtacctgaat aagcatcaag aagtttgtat ctctgttcaa
     5761 gaacctcctc cagtaaagaa tgaacagctt catcgtcttc gataatcaaa atagtttcca
     5821 gcaggaacac ctacttaatg tcagattttt ggaaaataaa cattcctaga gctgatgata
     5881 ccacgattgt tactagactg accagaatca ttttcggtgc ttgcgaataa gttgcttgtt
     5941 gcgcaaagtc cgtatacatg agccacgttt ccgtaaacca ctccgttaga aattccaagc
     6001 ctttgatttt tgcgaaaaca ccgggaacga aggccgttcc catgatataa agaaaagaaa
     6061 gaagcaaact gacactggta cttctagtga tgaatgtcaa agccactgga acagataatg
     6121 tcgctaatat cagcagaagc aatacaggaa atacttttag aacataattt ccatagtttt
     6181 tgattccctc aacttcacca aatccgaaag caagtgtgta agcaatcatg gctagccccg
     6241 taatcaaaac cacgccaagt gttgtgatca aaagcgtaac gagaagtttc gacacataat
     6301 aagtcattcg attccgtcct aaagataacg tattgcgaat cgtaccgttt tgaaattctt
     6361 ccccaatata aaagcacgca aatgctgcga aagccagaag aatccatgca gccatttttt
     6421 ccaccattaa aatccctgtt gcaccgttga tgactgtatc cgttgcttgg tttaattcat
     6481 tgccgtattt tgtatgcata taccattcgt tccaagaata aaaaagcgga agtacgacca
     6541 tcagtcctag tgagacccac attttacggc tcttccatac ttttattttt tcagcagtta
     6601 atagattaaa catgtggatt gcctccagtt aattgtaaga aataatcttc cagtttttgc
     6661 ccaactaggt ggataccatc cacaggaact tttgcctcta ccaatgcttg attgatggct
     6721 gctacttggt caattccttc aaaaagctgg ataacacggc tagattggac aaaataatcc
     6781 ctataaccaa gagagtcgag aaccgttact gcttgttccg tcgccgaagt gtcaattttt
     6841 ataaattgac gagattcttg tgccaattct tctttcgata attctttgat caagtgtcct
     6901 tcatgcaaaa taccgtagtg agtggcgatc tgtgacaatt catctagcaa gtggctagaa
     6961 agcaagacgg tgatgccccg ttcttttacc aaacgttgga tgatttctcg catttctgcc
     7021 atccctgctg gatccatccc attcgtcggt tcatccaaaa tcaaaaattc tggattcgtg
     7081 atcagtgcat ttgcaatagc taaacgctgg cgcataccta aagagaaatt ctttgctttc
     7141 tttttccctg tattttccaa tcgcatcatt ttcagcaggt cttcgatttc tctatccgac
     7201 acaccgccgt tcgctgcttg gatacgtagg ttatctcttg cagttaattc tggataaaga
     7261 gcaggggttt caatcgattg acccatacgt cgtcttgctg cctgcaaatc cgtcgttcca
     7321 aataattcga tcgaaccttc atcaatcgag atcaaaccca taatagttct cataaaggta
     7381 gacttacccg ctccattctc cccaatcaaa ccatagatca ttccacgttt gatttcgatc
     7441 gatactttat ctaatgcttt ggcagcaccg tattttttgg ttataccagt tgctttcaag
     7501 accgtttcat ttccaaaatc catacttatc cttctttctt attgataagt gaagtatagt
     7561 tgacgggtat aaagaaagca gccagaaatg tataaagaaa gtgtaaattt tccaattgaa
     7621 tttcaagaaa aatcagactg tctcgcagac gtggttgagt tgtctgccaa tcaaagagcg
     7681 caccaaacag tgctaatcca agtggactgg ccaccatggt aatcgttgtt atcgttgaca
     7741 tcagacgtcc ttgtatatgc gttggaactt ctcgctgaag ccagaccata taaaaagcaa
     7801 aagaaaacat taatccttaa aaacggactt atgaaaaata atacatgccg tttgggattt
     7861 acaattagct accaaataag aggttatgac aaaagacttt tcacaatccc ttcttccgaa
     7921 taaacggtgt tctcctgcca actcctcggt cacaagtcac aatatttatc aaacatgaga
     7981 agcaattttt gtaaattcct tcttatttct tgaagctgac cacttctgtc acaacctcag
     8041 aagcggataa ataatattgt ttgatgatac tttatctttt tcccgtttgt ttatagatcg
     8101 aatgatatta tagaccaaga atcctaccta ttaacatagc taaaaacaca aaaaaggcac
     8161 tttccaaatt gtggaaagtg ccagaaaagt taggattaag gtaactgcgc tcagcattgc
     8221 ttcgcccagt acgattctcg taactcgtga actcgaacga taatcgcaat taacgttttg
     8281 agaactgtga agctttacgg gctttcttaa gacctggttt tttcttcgta tcattgaata
     8341 agtttgaaaa gccttgattt ataaaggttt ttccgtaaat atattttgtt atatttgatt
     8401 aaatttgaaa tgtttgtgtt ctaaaatgtg ttctaattta aaggcagtat atttcgtatt
     8461 ttatattaaa tttttaggct cattcattct caaatccatt ctatttagtc aataaatatt
     8521 ttaaaaagac tttataaaca aagatgttca agcgagcatc ttattttttt gtgcctaatt
     8581 tgaaaggaga acatttcatg tataaaaaac aaaaaaagaa attatgtgta ttaactattt
     8641 tgagtttggc tgttcaatca attgtaggag caattgctcc tacgattgct tttgctgatg
     8701 aaattaccca tccacaaacg gtaaccgtag aactagattt agcacatcaa tatgctgttg
     8761 aaggaacttt tagtgatggt cgtccaatgt cagaagtcac tgttccgcat tatgcagttt
     8821 acaatggtgt gaagcaagat attttctgtg ttgaaccagg agtcccgatt tacaatgagt
     8881 tcactcctgg atatgagaag aacccgttac cagatatgtc cgataaagcc aaattagttt
     8941 ccgttttatg gaaaaaagta ggtacagatg cagatactca tattgtggca cagaagatga
     9001 tctggcaaga agttaatggg tatacccttc actcaataaa acgatcagat ggtagtgccg
     9061 taaatatcgc agccattgag gcaaaaatca atcaagccat tgccgattat cagaagaaac
     9121 caagcttcca taatagtacg gctaaaacag tattagggca atcgactact gtgacggata
     9181 cgaataatct gaatttgtca gagttcgatg aggttgtgga aaataccgca aacattgatt
     9241 atcgtgtgaa tggcaatcag ttgataatca gcccaaatgc caattcaaaa gaaagtggtg
     9301 tgttgacgct taagaaatct gctggtaccg gaacacctgt cgcctataaa aaggctggcc
     9361 aacagacttt aatggccggg gcgatcgata agccgaacac ttatacggtc aaaattgatg
     9421 tggaaatgga gggttctttg aagattaaaa aagttgataa agaatcgggt gatattatac
     9481 caggaacggt tttccattta gactttggaa aaactttacc cgcaaaagac gtgaccaccg
     9541 ataaagaagg cattgcgacc ttagatgaga ttcctcatgg tacaaaggta accattactg
     9601 aaaaatcggt gccggcgcct tatacgattg ataccacacc catgactgcc acaattaaag
     9661 caggtgaaac catttcagtg acttcaaaaa atacgcgaga aaaaggtcaa attattctgg
     9721 ataaaaccgg aattgaaaca gggagtgatc tttggaacga taattactct ttagcgggca
     9781 atacgtttgc cattcgcaag gatagcccaa ctggtgaaat cgtccaagaa atgaccacgg
     9841 atgaaaaagg tcatgcagaa acaccaaaag agattgccaa tgcgttggaa ttgggaacct
     9901 actatgtgac tgaaaccaag gctagtcatg gctttgtgaa tactttcaaa ccagtaaaag
     9961 tcgaattgaa ttatgtcaat caaacagtgg ctcttgttac cagtaacgta aaagggcaaa
    10021 accaagaaat tactggtgaa accactttga caaaagaaga caaagatacc ggtcatgaaa
    10081 ctcaagggaa agctgagttt aaaagagctg aatacacctt gtttactgtt aaggatggaa
    10141 aagcggtcaa atggaccgag aactttaaac cagaattgtt gaagggaacg aaagcttctg
    10201 atgaaaccgt aactttgact ttagatgaaa agaatcaagt tgccgttaaa cacttagcga
    10261 ttaatgagta ttactggcaa gaaaccaaag cgcctgaagg atataccttg gatgaaacaa
    10321 agtatcctgt atccatcaaa aaggtggatg atagcgaaaa aaatgctgtg attactcgag
    10381 atgttgcggc aaaagaacaa gtgattcgct ttggctttga tttctttaaa tttgctggat
    10441 caactactgg cactgccgaa actggcttca atgatctaac ctttaaagtg tcgccattgg
    10501 aaggaaccaa tgaaatcacc ggtgccgaag atgaagcgac cacagcttat aacgagcaat
    10561 tagggtttga tggttatggc aagtttgaaa atcttcctta tggggattat ttacttgaag
    10621 aagtagaagc cccagaaggt tttcaaaaga ttacgccatt agaaatccgt tctactttta
    10681 aagaaaataa agaggattat accaagagtg attatatctt tactattacc gaaaaagggc
    10741 aaaagcaacc gattaaaacg gtaacggtcc cttatgggaa tctaaccaat gagaatttct
    10801 cagtcagttt gaaccgtttg atgctttatg atttgcctga ggaagaagat agcttaactt
    10861 ctcttgcgac ttggaaagaa ggagataaag aattgactac tcttgattct accgagctag
    10921 tcgataaatt gagctataat ctacatgaaa tcaaagaaga ctggtatatc gtagctcaag
    10981 ccatcgatgt agatgctaca aaagctgccc aagaaaaaga taaaaaagcc aaaccggtgg
    11041 cgattgcgga aacaaccgca actttagcga ataaagagaa aacaggaact tgggagattc
    11101 tgcataaatt aactgtcgaa caagttttag acaagaccat tgtcttattc aactatgtgt
    11161 atgaaagcaa agaagcctat gaagctggtg atgagccggt agcgaaggat gctagcttga
    11221 acaatcaagc gcaaactgtt aattgtatgg tggaacgtca tgtttccatc caaaccaaag
    11281 cgcacttaga agaaggttct cagaccttta ctcatggtga cgtggtagat atgtttgatg
    11341 atgtatccat tacccatgat gtactagatg gctcaaaaga agcttttgaa acaattctgt
    11401 acgctttatt tccagatggt acaagtaaag aaatttggaa atctggcaag attgattacg
    11461 aagtgaatga caaagaattt accaaaaccg tacttgcgga aaaagtggat acaagtaaat
    11521 atccagaagg tacaagtttt accttcaaag aaattaacta tgacaaagat gggaatatca
    11581 atggaaaaca caatgaagat ttgaaagaaa aatctcaaac tttaacgccc aaaaaagttc
    11641 cgacaacacc aagtacacca gaacaatctg aaatgccaac tgttccaagt ggctctcaag
    11701 aatctagtcc tacagtgaag gcattccctc aaaccgggga gaaaacttcc aatgtgctac
    11761 tgttcattgg ctttagcttg atctttgcga cggctggcta ttatttttgg aatcgtcgga
    11821 actaaggtga taagatgaaa catgtaaata gtagaaaaca atacgcgcga ccgcctccta
    11881 aaaaagtggt cgcgctattt ttgtattcaa aactaacaat gaaaagagga aaaaatatgg
    11941 aattgaaatt tgttgtacca gatatggtcg agacgttcgg aaaactaaca tacggtggtg
    12001 aaggtgaagt tctatcggaa gggtatggtc gaaatactac aattgttggt cgaagttacc
    12061 atctgttttc aaataaacaa cgggccgatg atattgaagt ggtggtagct gcggaagccg
    12121 gcgaaaagga ttttgaccaa gatcaaccac taaaagctgt aaatccccac ttaattgcca
    12181 aaggctatga aattgagaat cgtgggttta ccgattacgt gttgtatgtt gatgatttag
    12241 tgaaagcata ggaggaaaag aagatgagat taacagaagg tattgtcgta gattcaggat
    12301 taacctttgg caagttgcgt ttttccgcat tacgtcgaga agtacgcaag caaaatgagg
    12361 acggaacggt tagtaacgag gtaaaagaac ggacctataa tttgaaatcc tccgcgcaag
    12421 gtcgaatgat ccaagtcagt attccagcaa atgtaccctt acgcgagttt gcttatgatg
    12481 cagaggtaga gctggtcaat ccaatcgtgg atacggtcgc caattatgtt tttcgggaag
    12541 gaaccaccgt taattggttt atcaaggcag atgatttagt cttgaaacga caactgaatc
    12601 aaggaaaacc tacgaaccaa aatgaaggaa agaaataggt gaagaatatg gaaattaatg
    12661 ttgaacatat tttagattgt ctcgatcaat ttggcaaagg tgagctaacg gaggaacaac
    12721 tcactaacgc attaacctat gatgaaaaaa tgtttctgat tatgcatcaa gggttactgg
    12781 aagtaggtaa cgatgccaaa gaagactttg atgtactgga ttggttaggg gaagaagagt
    12841 ctttctttat ggttgtcgag gtaaatgaaa gtttatgtcg gcaggcagaa tctgttttgg
    12901 aagaaatcga tgtggagatg ccggatgcca tcgaaagatt tttaaaacaa ttagtggaaa
    12961 cgaaacaact tcccgtagta gtaaatgact agcataactt tttgaaattg cccaatgaaa
    13021 tcctattggg ctttttcgac tggaggtgga aaccatcaaa atgtacaaag gacatcgcat
    13081 acgcgcagga gatcaacact tgatttatca ctttgttctt ggctggctaa tctttttgtt
    13141 cattggttgg atgagcgtct tttatttaca agaacttaga caatttgata tttctaaggt
    13201 atcattttcc actagtgaaa ttggctggtc tatgaaggat gtaatctgct tgctaggaag
    13261 tctcgctctt tcaggaagca tgatgttact ttatatacac ttttttcagg atcattggcg
    13321 aagtctttgg catcgacaaa agttagctcg gatgattcta gaaaaccatt ggtatgaagt
    13381 aaagcagact caaggtgaag gttttttcaa agatttgaat agtagttgga ccaaagagag
    13441 tatcagttac ttccccaaaa tttattatcg aatgaaagat ggcttacttt ctattcgcgt
    13501 tcaaatttca ctgggaaaat atcaggatca gctattgaag ttggaaaata agttagaaag
    13561 cggactgtat tgtgagttag tagaaaaaga actcaaagac tcctatgtgg aatacacctt
    13621 gttatacgat atgattgcca atcgaattgg gatagaagaa atagtggcag aaaatggggc
    13681 gttgcgattg atgaaaaatc aagtatgggc gtatgattcc ttgccgcata tgttaattgc
    13741 tggcggcaca ggtggtggga agacctattt cttactcacc attattcaag cactattgaa
    13801 gtcagatgca gaattgttta tcctcgatcc caaaaatgcg gacctcgccg atttaggtac
    13861 gataatgcct cacgtatact cccaaaaaga agaaatttct gaatgtgtgg aagattttta
    13921 cgaacgcatg atagctcgta gtaggtcaat gaaagaaatg cccaactaca aaacaggaga
    13981 gaattatgcg tatctcggac ttccgcccaa ctttttaatc tttgatgaat acgtggctta
    14041 tatgggtgcg aatcgttttc ctacaagcat cgaatagatg tgaaatggaa gcctattgag
    14101 attatcaata gtgacaacta atgttttgag agatggaatg aaagccttca agttgagctg
    14161 aaacatcccc gctaatactc ctacatgctc taatcaatga agtagattgg ttttggtatg
    14221 aagctaggtg aagtcgtctg aaatctgcct aagtgcttga gcataaggct gactataggg
    14281 cgggcggctg aaaaagataa ctatggtaag aatgtctgaa aatgactgac gaatctgcga
    14341 atgtacgggt ctaaaaggct gatcttattg aaagatagga acatacggac gtatggtgag
    14401 ataccgacaa gtaaggttcc atgatatgaa aatcgggata gctaacgaag aagctataat
    14461 ttcacaggct catagtagat acctaaggtt atacatgata gctaaaaaca ttggaacgat
    14521 gtaagcagaa aactatcaat taaatgacta cttctagata ggtgcatata agtaataacg
    14581 aaattgcttt gtttctgtga aagtaagggc acagtaccga tgaagcgtct aacaaatgtg
    14641 gagggatagc cctaagtcta tttaataaca atgaatgttg aaacagatga agttcaagga
    14701 tctggtaaga acgtgggaaa atccgttatg gaggtgccac gcttgaacac aaaaatacgt
    14761 tactgggaat attatgatct acaagaaaca ttcgatcagc tatttactca aagtagaaac
    14821 gggaaaaagt tttatcaatt atatgagtta atcatttctg aaaataatat tcttttagca
    14881 taccgtacta tcaaggcaaa caaggggtct tcgacaccag gaacggattc cttcacaatt
    14941 gataactata aggaaatgaa ccaagcagag tttatccatc ttattctcag tcatttagaa
    15001 aattacaaac caaaatccat aaagcgagta atgataccta aacccaatgg tgagaaacgc
    15061 ccattaggaa ttccatgcat gattgatcga atgatccaac agatgttcaa gcagatactg
    15121 gagccaatat gtgaagctaa gttttatgaa catagctacg gtttcagacc attgagaagt
    15181 gcaaagcatg ctctaggtcg cattatgtat ctcataaaca tcagtaaaat gcactatgca
    15241 gtagacattg atatcaaagg tttctttgat aatgtaaacc accgtttact gataaaacaa
    15301 ttatggaata taggaatttg tgacaaacaa gtcttagcta ttctttcaaa atctctgaaa
    15361 tcacccattc aaggagaagg tatcccgagc aaaggaacga tccaaggagg tatcatttct
    15421 ccactattat ctaatgttgt tctaaacgac ttagaccatt gggtctcaaa gcagtggcat
    15481 acctttgaaa caaagtatcc ctatactaaa ggttacaata aatttcgtgc tctaagagat
    15541 acaaatctaa agcaaggcta tattgtcagg tacgcagatg actttaaaat catgactaat
    15601 gattatccga cagcattaaa atggttccac gctgtcaagc tttacctaaa agacagactt
    15661 aaattagata tttccaatga gaagtctaaa atcgtcaatt tgcgtaaatg taagtcggaa
    15721 tttttaggat tcgctatttg cgtgaaacaa aaagggaaaa aatgggtttg taattcacgt
    15781 atttctaaca aaaaaaagga tcagattaaa gaagagatta aacaaagaat caaagatatt
    15841 caaaagagtc ctacagcaca aaatgctctc ctgtttaatg ctctcgtgct cggtgttcat
    15901 aattatttca ggtatgccac tcatattaat ttagatttga ataggatgaa ctacgttctt
    15961 tcattcacta cttacaaccg attatcaaat tgtagtacaa gatgttaccc caacaaagct
    16021 tccccaactt acaaaaagtt ttactcaaca acgatgaaaa cgtataaagt agctggagtc
    16081 tatttgtacc ctctttgcga cgtatctaca aaaacaattt acggatttaa tacggaggat
    16141 acaccgttta ctccgtttgg tcgtcaaaga ttggagcaca aaagcctaca gtctcttgtg
    16201 ttccaagaat taagaaaatt gatggaatcg aaaataccga atagaacggt tgagtattta
    16261 gatagtagaa tatctcgtta ctcaatgaaa tcaggcaagt gtgagattac aaaacaattt
    16321 ttgccagcaa aagccgttca ctgtcatcac tatttgccaa aaagtcttgg tggagacgac
    16381 aaattcgata acttgagaat tattcataag gatatccatc ttctgatcca tacaacaaat
    16441 aaaatgataa tcgatcacta cgtgcatgaa ctgaaactgg tgccagagca aatagcaaaa
    16501 atcaaccttt atcgaaagat gtgtaatctg caaaacattc aatagaaaat aaatagatgg
    16561 aacgccgtat gaggtgaaag tctcacgtac ggtgtggagc gggggaaaag atggagatta
    16621 tatcaaattc ttacctatcg ctatagatgt taacgacaaa agaaagtgcg gtgattttga
    16681 ataagcttaa acaaatcgtg atgttaggcc gtcaatctgg tttctttctg attctagctt
    16741 gtcaaaggcc ggatgcgaaa tatctaggag atgggattcg cgatcagttt aacttccgtg
    16801 tggctttggg tcgtatgagt gagcttgggt attccatgat gtttggagaa gtcgataaaa
    16861 atttttttat gaaacgcatc aaaggacgag gttatgtaga tacagggggc agtgtgatta
    16921 gtgagtttta tacgccactt gtaccaaaag ggtatgattt cttaagggaa attagcaaca
    16981 ttgttggatt aaccgttcat acggaaagag aaaataatag tgtgtagcct aggaatatca
    17041 ataatcctgg gcttttttat tgaggtgatt tatgacattt ttagatttat tcgccgggat
    17101 tggtggcttt cgcttaggta tggaacaagc cggtcaccag tgtatcggtt tttgtgaaat
    17161 tgatgaattt gctcggagga gttataaagc aatccatgat acaaggaagg aggtggaaat
    17221 gcatgacatc acaagtgtat cagacgaatt tgttcgatcc ctcggaccag tggacatcct
    17281 ttgcggcggc tttccgtgcc aagctttttc aattgcggga aagcggcaag gattttccga
    17341 tactcgaggt actttattct ttgaaatcgc tcggttcgcc actcttctcc aacctaagtt
    17401 tctattcctt gagaacgtcc ggggattgct caatcacgaa ggaggggcta cgttcgagac
    17461 gatcctccga acgttggatg gattggggta tgatgtggaa tggcaagtgc ttaactcaaa
    17521 ggcctacgtt ccccaaagcc gtgagcggat cttccttatc ggacattctc gagatgcatg
    17581 taccgaacaa gtatttccta tcattggatc gtcttcaaca tctgatcaaa acatcagaaa
    17641 cttggtaaat atcaatcctt ccaatcgagg aatgggtggc caagtctatg gttcagatag
    17701 cgtagcacca accttgaccg gtgatgaagg aattaaaatt gccttaccgg taaaggatgg
    17761 gatttctgta gcgggtatgt tgtcgggaaa ttttgaacaa ggcaatagga tttatgaggt
    17821 aaccggaaca gcccccactt tgtcaacgaa acaaggggga acgaagatta tgattcggaa
    17881 aaatgaaaca gaatatcaag aggtgaagcc aggggacagt gtgaacctgg cttttcctaa
    17941 ttccacaagt cgccgaggtc gtctgggaaa acaaagtgtg cataccttac taacaggaga
    18001 ccaacaagcg gtagtcacgg atcagtacca gattcgaaag ttaacaccta gggagtgctg
    18061 gcggcttcag ggctttccag attgggcttt tgatcgagct tctcaagtca attcagatag
    18121 tcagttgtat aaacaggccg gaaactcagt aacagttccg gtcatttttg atattgctag
    18181 aagattaaag ggggtaaaag catatgattt atgacgagtt gattggagaa atctactgga
    18241 tgattggcaa gattcaggca gatccgaaat tggaagaaga actgtaccgg ctcaattttg
    18301 atattcgcaa aaacggggtg aaagtccagg tgattcgtat gtagtggatg aggaaacgga
    18361 tgctcgaatg gagctcaatc aagtcatcgg tgaattggaa cgaattgcgg accttgccaa
    18421 agaacctgat atccgccaat acttgtttga gataaaagca gagttggaaa ttgagggtat
    18481 caccgcggag taatagggaa ataacccact gataggcact gaccgcgggg gatttgcgaa
    18541 gcagaaatcc tccgtggtgc tgtgcctgtt catggacggc ggcagccggc catgatgtct
    18601 caccccccgt atctaacagg ggggtacaaa aacacaaaaa acaagcaagt aaccataaaa
    18661 aatgcttgtt gtatcaagga tattaagtaa tttgtatagg tgttaactca tttggaaaag
    18721 ttaacacctt gaatttggag gtgaaaactt ggcgcaacga aatttagatt atcggttatt
    18781 aaaagaacga cgcaacgaat atggcgtttc acaaaataaa ttagctactg cttgcggact
    18841 tagccgccca tacctaaatc agatagaaaa cggtggcgta atcgcctcta caaagaccat
    18901 gagaaaaatt tttgatcaac tggaaagttt caatcccgat ttacctttaa cgttactatt
    18961 tgactatgta aggattcgct ttcccacaac ggatgtgcgg aaaattattc aagaaattct
    19021 ccatttgaaa ttcgagtata tgcttcatga agattacgcc ttttactcct atcaagaaca
    19081 atatgtcatg ggcgatattg tagtgatgtt atctcatgaa gaagataaag gtgtcctttt
    19141 ggaattaaaa ggtcgtggtt gccggcagtt tgaaactttt ttactcgccc aaaagcgtag
    19201 ctggtacgac ttttttgaag attgtctaaa agccggtggc gtgatgaaac gcttggattt
    19261 agcaatcaat gatcgagtgg ggctactgga tattcctgat ttaacgaaaa aatgtcagaa
    19321 ggaagaatgt atctccttgt ttcgtacctt caaaagttac cgttccgggg aactgttgaa
    19381 agctgatgaa aaggatggta tgggcaatac cttatatatt ggcagcctca aaagcgaggt
    19441 ttatttttgt ttgtacgaaa aagattatga acaatatatc aagttaggga ttccattgga
    19501 ccagaccgaa acgaaaaatc gttttgagat tcggttgaaa aatgaccgtg cttattatgc
    19561 gattcaagat ttgttaaaag gtcgtagcat tgaaagtacc acgttttcca ttatcaatcg
    19621 gtatttgcga tttgcggata aagtagatgg aaaacggcga accaattggc ccttgaatga
    19681 acaatggggt cgttttattg gacggaatcg gaaagagatt caattaactt ctgaaccgaa
    19741 accgtatacc attgaacgaa cattaaattg gctaggtaga caagtcgccc ctacgtggaa
    19801 aatggctaag gagttggatc gattgaatca aacaacgtat atccaggata tggtacagaa
    19861 tgcgcgactc tcggaccggc ataaaaagat tttggagcaa caaagtatgg caatcgaaaa
    19921 cttgattgta tgaaaagagg aattttgatg gaaattgtaa aagatatgtt attggtactt
    19981 ggtggcagta tgcttggtgt taccgtgatg tgcttgatga atgcaagtgc agcagcggat
    20041 tgagcaatgg aaaagaaagg aaagtgacaa aatgaatttc ggacaaaatt t
//
LOCUS       DANKHL010000033        19795 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000033 DANKHL010000000
VERSION     DANKHL010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.035"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..149)
                     /locus_tag="PW101_000826"
     CDS             complement(<1..149)
                     /locus_tag="PW101_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDL2513249.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDD"
     gene            465..1124
                     /locus_tag="PW101_000827"
     CDS             465..1124
                     /locus_tag="PW101_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2513250.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            1209..2114
                     /locus_tag="PW101_000828"
     CDS             1209..2114
                     /locus_tag="PW101_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2513251.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(2116..2748)
                     /locus_tag="PW101_000829"
     CDS             complement(2116..2748)
                     /locus_tag="PW101_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL2513252.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    2899..3024
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            3068..3592
                     /gene="infC"
                     /locus_tag="PW101_000830"
     CDS             3068..3592
                     /gene="infC"
                     /locus_tag="PW101_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDL2513253.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            3664..3864
                     /gene="rpmI"
                     /locus_tag="PW101_000831"
     CDS             3664..3864
                     /gene="rpmI"
                     /locus_tag="PW101_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDL2513254.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            3917..4276
                     /gene="rplT"
                     /locus_tag="PW101_000832"
     CDS             3917..4276
                     /gene="rplT"
                     /locus_tag="PW101_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDL2513255.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            4528..5865
                     /locus_tag="PW101_000833"
     CDS             4528..5865
                     /locus_tag="PW101_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HDL2513256.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            5913..8042
                     /locus_tag="PW101_000834"
     CDS             5913..8042
                     /locus_tag="PW101_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDL2513257.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            8017..8706
                     /locus_tag="PW101_000835"
     CDS             8017..8706
                     /locus_tag="PW101_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513258.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(9114..9727)
                     /locus_tag="PW101_000836"
                     /pseudo
     CDS             complement(9114..9727)
                     /locus_tag="PW101_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(9863..10057)
                     /locus_tag="PW101_000837"
     CDS             complement(9863..10057)
                     /locus_tag="PW101_000837"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513259.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(10107..10547)
                     /locus_tag="PW101_000838"
     CDS             complement(10107..10547)
                     /locus_tag="PW101_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDL2513260.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(10551..11396)
                     /locus_tag="PW101_000839"
     CDS             complement(10551..11396)
                     /locus_tag="PW101_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDL2513261.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11545..12963
                     /gene="pepV"
                     /locus_tag="PW101_000840"
     CDS             11545..12963
                     /gene="pepV"
                     /locus_tag="PW101_000840"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDL2513262.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(13020..13967)
                     /locus_tag="PW101_000841"
     CDS             complement(13020..13967)
                     /locus_tag="PW101_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2513263.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(14190..14540)
                     /locus_tag="PW101_000842"
     CDS             complement(14190..14540)
                     /locus_tag="PW101_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL2513264.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            14740..15819
                     /gene="pepA"
                     /locus_tag="PW101_000843"
     CDS             14740..15819
                     /gene="pepA"
                     /locus_tag="PW101_000843"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HDL2513265.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            15963..16283
                     /locus_tag="PW101_000844"
     CDS             15963..16283
                     /locus_tag="PW101_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL2513266.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            16305..16769
                     /locus_tag="PW101_000845"
     CDS             16305..16769
                     /locus_tag="PW101_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2513267.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            16974..17579
                     /locus_tag="PW101_000846"
     CDS             16974..17579
                     /locus_tag="PW101_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDL2513268.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(17618..18907)
                     /locus_tag="PW101_000847"
     CDS             complement(17618..18907)
                     /locus_tag="PW101_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDL2513269.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            19335..>19795
                     /gene="rlmH"
                     /locus_tag="PW101_000848"
     CDS             19335..>19795
                     /gene="rlmH"
                     /locus_tag="PW101_000848"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDL2513270.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAG"
BASE COUNT         6283 a         3666 c         4053 g         5793 t
ORIGIN      
        1 tgatcgtcca ttgcatccag ctcttcaagg aatgcggtga cttttgttgc atagaaagga
       61 aaggcaatcg ctggttctcc ataaacaacc gcgtgctcgc ccatcaatat gatctttccg
      121 cttgattctc cttggccata gtttgccata atctaactcc ttctcacgta aaagtgattt
      181 ttaagcaaaa aataaagcgc ccaaaacgag ttgcgctgct tcacgaattt ttttgtccac
      241 taagggtctc aaaaaacatt cctcctttat tttactaaaa ccatctcaga agtaaaacta
      301 agaatacgtt ttctaaagaa aaaacacttt tttcttaaga attttttgac tttttttctt
      361 ctttgactag aaaaaaatac aattgacact actagatatt gggggtatat ttaaaaaggt
      421 tggagttaaa cacaatattt agtgatgaag tgagctggag gtaaatgagc gtgatcgttt
      481 tagcaggtac aattggtgcc ggaaaatcaa gtttaacaga aatgatggct gaacactttg
      541 atagtcaggc attttatgaa tcgattgatg ataatgaagt cttgccctta ttttatgcaa
      601 accctgaaca gtatgctttt ttgctgcaaa tttatttttt gaataaacgt tttgcaagca
      661 tcaaacaagc aatgaaagat gacaataatg tattggaccg ttcgatttat gaagattcac
      721 tgcttttcca tctaaatgca gatctaggaa gagcaacaga aacagaagtt cgtgtgtatg
      781 atgaactgct ggaaaacatg atggaagaac ttccctatgc tgcccataaa aaacatccgg
      841 atttattagt tcatattcgt gtatcttttg atacgatgtt ggagcgtatc gaaaaacgtg
      901 gtcgttcgta tgaacaattg tcctttgatc caagtctata cgattattac aaagaattga
      961 acagacgtta cgatcaatgg tatgaagaat acaaagaaag tccaaaaatc caaatcgatg
     1021 gagaccgcta taattttgtg gaagatcctc aagcaaaaga agaagtcttg aaaatgatcg
     1081 aagaaaaatt agcagagatt cgccaaacca aagtagccat ttgatttaca gttgctcaaa
     1141 taaagggtaa acctttattt gagcgacttt tttctttata ctggaaacaa atgtcaggag
     1201 gatagagtat gcgggaaaaa cactatttta atcaagaccg taatttttgg atcaatgtac
     1261 agcaaaacga tgtgctagct gttaaaaact taaaagaaga atatggcatt agtgacgaga
     1321 tgctgactta ttctctagat aaaaatgaac gggcgcgcgt agagtacgat tcttttgatg
     1381 aagcgttact gcttgtatca aatgttccgc accagcaaaa agtcgataat cattatgaaa
     1441 ctagtcctat cgcttttatt ttaaaagaag acggtttgtt tacatttacc acgccgaata
     1501 ccgagtacgt gattcgacta attcgatcgc ttttggaacg aatgccagac atgagtgtat
     1561 acagtttatt gtttcgtaca ttgtttttga tttctgattc cttttttcct ttaatcgagg
     1621 aagtgaatag tgaacgtcag cggcttaatc tgaaattaag agaaaaaaca acgaacaaga
     1681 atctgctgca actttcagat ttagagatcg gtttggttta cttagttaca ggaacaaaac
     1741 aaaatgtagt acttttagaa caaatcaagg cattggctat ttatcgcaag ttgtcagaaa
     1801 aggaaaaaga agagttggac gatgcgctga tcgaagcacg acaagcagtt gaaatgacga
     1861 acttagcttc gcagatttta gaccagctct ctggcactta taataatttg ctgaataaca
     1921 acttgaatga cacgatgaaa tttttgacgg tctggtcgct gatcttgacc gttccaacta
     1981 ttgtgacagg attcttcgga atgaatctcc agttaccctt tacccattcc gttttcggat
     2041 ggggaatcgc cttgatcatc agtttggtct tatctatttg gatgcttatt gcactgtggc
     2101 ggcggattcg ttgatctagt ctttgatctg caaaccaagg gcttgggcga tcacaagtgc
     2161 ttgctcttgg ttgacgatta gtcccgccaa agaatcttga gataaagcga ttgctgtgaa
     2221 gaaattagtc gtaaagtcta atcccttcag agaagttcga aaccagttcg aaccggtcaa
     2281 ctgattgtca gacaaaaaaa ggtttttcca agtgacctca taaaattcgc tgtcttctaa
     2341 ttgacaatga tcgaaggaga cagctttcaa gtttgtacta ctaaaggaag ccatcgaagc
     2401 aatacaatca gtgaaagtac agtctcgaag ataactttcg gcaaaattcg tacctgttaa
     2461 tttacattga tgaaacacga cttggtggaa actgccacca atccactcca gattggaaaa
     2521 atcacatttt tcaaaaatga cattactgca ttcaaaatga cgtaaaactg ttttttgcat
     2581 cgtaagtttt tggatatgac tctctcttaa taccatattt ttggcatcga gatagctttg
     2641 gtcacccccg ttgatcagaa gaccagagaa gatctcctca tcttctaaat aaaactctcc
     2701 ctttgtaaga agaggaagag aaggtggaat aggttcgatt tttttcatgt gggttcacct
     2761 cgtttttctt tagtatagca aagaagcggg aatcactgct agaaagtctg ctaatctaat
     2821 cattttcaga aaaggaagac aaactgtatg aaaaacattg acaaaaagat tagtttttgc
     2881 tatactatga tggttgagaa ataaaagcag aagcacccgc ttctcgcctt agttgataat
     2941 gtcactgggc tagatagttt tcactggttc tattaggtga agtacgcgcg ggttctgtaa
     3001 gagaactcgt ttttttattg ccgtttattc ggtaagcttt tctttctctc ataatatttg
     3061 gaggtgaatg accatagcaa aggatatgat ggttaacgac ggcattcgtg cacgagagtt
     3121 acgattgatc ggttcagatg gtgagcaatt aggagttaag acaaaagcag aagcattgaa
     3181 tattgcagaa caagcaaact tggatcttgt gttagttgcc cctggtgcga aaccaccagt
     3241 tgcgcgaatc atggactacg gtaaataccg tttcgaacaa caaaagaaag accgcgaagc
     3301 gcgtaaaaaa caaaaagtga tcaatgtaaa agaagttcgc ttaagtccta cgatcgactt
     3361 gaatgacttc aatacaaaac ttcgtaatgc acgtaagttc ttggaaaagg gcgataaagt
     3421 gaaagcttct atccgtttca aaggccgtgc cattacccat aaagaaattg gtcaaaaagt
     3481 cttgaatcgc ttagctgaag aaactgcgga tattgcaaca gtggaacaaa aagcgaaaat
     3541 ggacggacgc agcatgttct tgacgctggc accgaaaaac gacagtaagt aatttgaatg
     3601 actaactggc tgttggacag ccgatagtcg gataacgaaa gattttgagg aggaaatata
     3661 gtcatgccaa aacaaaaaac acaccgcgga tcagcaaaac gtttcaaacg tactggtaaa
     3721 ggcggattga aacgcttccg cgcgtttaca agtcaccgtt tccacggcaa aacaaaaaaa
     3781 caacgccgtc aattgcgtaa agcaagcatg gtttctagtg gcgacttcaa acgtattcgc
     3841 caacaactag caaaaatgaa ataagaagta caaataacag aatctgaaga tttattttta
     3901 ttaggaggaa ttatacatgg cacgtgttaa aggtggaaca gtaactcgta aacgtcgtaa
     3961 aagagtgcta aaattagcaa aaggttacta cggttcaaaa cacactttat ttaaaacagc
     4021 aaaagaacaa gtaatgaatt catacaacta cgcatatcgc gatcgtcgtc aaaagaaacg
     4081 cgacttccgt aaattgtgga ttgctcgtat caacgcagca gctcgtatga atggcttgag
     4141 ctactcaaaa ttaatgcacg gtttgaaagt tgctgaaatc gacatcaacc gtaaaatgtt
     4201 agctgacttg gctgttaacg atgcagcagc atttactgca ttggctgacc aagcaaaaga
     4261 agctttggct aaataagcta aacaattata aagaggaaag aagataacga aaagttatgt
     4321 tctttcctct ttttttattt acaatcctcc atcaaatgaa acaggacaat aacgtataag
     4381 tctaaaatag cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta
     4441 atgaaagcgc taactacata agttagaatg agtttgactg atatattagt ttgcttatca
     4501 gaaaaacaaa atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt
     4561 atctcattct ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt
     4621 gccatcatgg ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac
     4681 ccggcgatgg aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca
     4741 gctgagtacg ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt
     4801 ctagtagata gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac
     4861 aagccaagaa tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg
     4921 acaggaatcg gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta
     4981 ggtatttcag taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt
     5041 gctaatatcg tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct
     5101 gaaagctata cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta
     5161 gttgtggtac taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca
     5221 gcaaacgtac cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta
     5281 ccagttttag gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt
     5341 atctgggcat tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag
     5401 tttgcgaaat tatttacaga tggcgcagta gatgttgcac caatggttgg tttcttgatg
     5461 accttagcga tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt
     5521 ggagattgga taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca
     5581 ctaggatttt tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc
     5641 gtattagctg ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca
     5701 acgattgctc ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat
     5761 acaaaagtaa cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga
     5821 gcgatcttat gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga
     5881 taaaaacgat tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg
     5941 atgttggcgg tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg
     6001 gaaaatcttc agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc
     6061 agtcttttca aaattgtttg cgggaaaata atatcagtcc agaagatgtt gtgtttgttg
     6121 cacattctac cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg
     6181 taatcgggat ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga
     6241 acgatatcga tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca
     6301 aacacttaaa tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg
     6361 aggtcttggt ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt
     6421 atgaagcagc atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat
     6481 atggattgac aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc
     6541 tagatacggc aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga
     6601 tgattatgcg cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt
     6661 taaccatgct ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt
     6721 ctaatggtgt ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg
     6781 gacgtccagc aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag
     6841 atgttcgggt tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca
     6901 tcgatgtcgg tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga
     6961 cagaaaaaat caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg
     7021 attacgtcaa ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag
     7081 ccaatgtcct tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac
     7141 gaaaagccat ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac
     7201 agatcatgga aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt
     7261 atcacttgga gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga
     7321 ttacttactt tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga
     7381 tttcttctat tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt
     7441 cgccaagtaa agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat
     7501 caggcgctac accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag
     7561 taacggctat cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca
     7621 caacagaaga agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt
     7681 gcttattgga aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg
     7741 ctggaagtct tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt
     7801 catgtatgaa aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata
     7861 tggcagttta tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa
     7921 gagtctctga ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag
     7981 tatctactat ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat
     8041 aagttagaag agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac
     8101 cagtttcggc gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata
     8161 caacaatcaa tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt
     8221 gccgatgtag gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag
     8281 caaataaacg aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg
     8341 acggagccgt tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg
     8401 caagctttga ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt
     8461 tatacgcaaa gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac
     8521 attcgtgcat tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat
     8581 ttttcgcctt atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa
     8641 gggatcttag aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac
     8701 aaataaagag actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacgatgtt
     8761 ctcctgtcaa ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt
     8821 gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg
     8881 ttcaaaagct tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt
     8941 ttacttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg
     9001 gtgtgcaatc agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta
     9061 ttttgccgtt tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg
     9121 atgggtctgt cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc
     9181 tcctcgataa tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga
     9241 tcaatcattg attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct
     9301 gaaatcgtca aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa
     9361 tcccaaattt ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa
     9421 tcaaaaaaag cttttttatt ttgagcgatt actgcctgat cggtttttcc aacttttccc
     9481 gcgtataacg ggctaatgaa gcagatcgga tatcgatttt tgaaacataa gtgccggact
     9541 gtggaatggt ttttacaagc tcttgattgc gcagttggat cagtgcttct cgaatcggtg
     9601 tacgcccgat agctagttgt tcttctaaca ctttatcgga gattttactt ccaggtgcta
     9661 actcggaata aatgatttgt ttacgtatag attcataagc ttgctgttgc aaattattga
     9721 taaccattta accttccttt ctatgcagat ataatctatt ataacataaa tcgcttacaa
     9781 atcgtgtggg cataaaaaca cagtctgcaa aaaaagccct gataggcatg cctattagag
     9841 cctcgaaaag aaaaatattg aatcaatctg gtcgtacctc ttgtatcggt ttgacacgac
     9901 cgccgattct tttagcaaaa gcttcggatt cttttttgtc tttgaaaatg agcagattgt
     9961 ctaagttttt ttcccgagtg cgacagccag ttttatcgat gtaaccgtgc attactttaa
    10021 cgacatacat ggggatcacc tgcttttttc ttttcattat acgaactact cgacacaata
    10081 caagaaaaat gcttagacgg tctttattaa gaatttacat catccaaaga agcgtacgct
    10141 tggaataact gatctaactc cggattgact ttgctcagct tcactaatcg ttgcgaatca
    10201 gaaagtaaaa aacagtgttt actactgcca gtagttttta attttttgtc ttcagttccc
    10261 acaatttcgt agctaaaatc caaccgtgtc cccgtatact tttggatagt tggatggatg
    10321 ctcacttttt ctccgtaatg gatcatttct ttatagctgc aatttacttc cagaacgggg
    10381 atgatgatcc cagattcttc gattttttta tagggaaacc ctagatgtac caataaggct
    10441 acccgagcct cttcaaacca gcggatataa ttgctgtgat gaataatccc catttgatct
    10501 gtttcataat aaaaaggttc tcgcacataa cctgtaaatt tctccatgac tcatgcctcg
    10561 ttttccattg attttttaca aaaataattc atccatttcg gaagtgcttc gctgatttgt
    10621 gtcggcaaaa caatatattg ttcttttgcc agctggtctc caatgaaact atgaagataa
    10681 atagctgctg caatcgtttg gtcattttga ggaaactggg ctaaaaatcc tgtgatcatc
    10741 ccagctaaag tgtcacccat gccgcccgtt gccatagcag cagaacctaa aggattggtg
    10801 tagaagtgct ggtgaccata aatttttgtc tcatgacttt tcaaaacgag cacagcaccg
    10861 atttcctgtt gcttcttttt gttttcttta tcccgttgtt cttgtatcgg tagagaactc
    10921 aaccgttgcc actccatttg atgaggtgta agtacagttt gttcgggata gggtagcaaa
    10981 agatgattag cagcaagtaa acttaaagca gagccatcaa tcaccagcca ttgttctttt
    11041 gattggtttt ctagtacttg tttgaaacgt tccaaactct tagaagaagt acccaatccg
    11101 ggaccaatca agagtacatc tgcttgcttt attacggaaa gaaactggtc agtatccgtc
    11161 caaactacaa tcattgcttc tggcagatga ctgtgaagag cactccgatt tttttcatca
    11221 gttactacag tcgtgagacc cgctccgctg tgtacggcag ctaaagcact cataatgatt
    11281 gctccgccat attgttcatt gccgccaatc aaaacgatac gtccaaatgt tcctttatgt
    11341 gaaatttttg gacgaggtgt gatgactctt ttcactagtt gttcgtttat cagcatctaa
    11401 aaacctcctt ttactcatta tagcgtatat tttttttaga gaaaaacaga taagaaaagg
    11461 aattgtcaga ttttttgaac tatttttttc tgtaatttgt ggtatcatga acacgaagac
    11521 ttaaataagt ggaggattaa ctttatgaca attgattggc aaaaagaagt agaagcacgt
    11581 aaagaagact tgcttgaaga cttgaaaaat cttttacgtg tcaacagcga gcgcgatgac
    11641 tcaaaagtaa caccagatgc accatttgga cctggacctc gcgacgcatt gaaacatatg
    11701 ttagcttatg gcgaacgtga tggatttaaa gtaaaaaatg tcgataacta tgcagggcat
    11761 attgatcttg gcgaaggaga cgaaacgcta gggatatttg gacacatgga tgtcgttcct
    11821 gcaggagatg gctgggacac agatccttat gaaccagtaa tcaaggatgg aaagatcttt
    11881 gcacgcggat caagcgatga caaaggtcca agtatggcag cttactatgc gatgaagatc
    11941 attaaagaac ttgacttgaa actatcaaaa aaagttcgct tcgtagtcgg cagcgatgaa
    12001 gaaagcggct ggggcgatat ggcttattat ttcgaacatg aagaagaacc agattttggt
    12061 ttctcaccag atgctgaatt cccaatcatc aatggtgaaa aaggaaatgt ttctttagct
    12121 ttacgcttca aaggcgacaa tgcaggagat tatgtcttga aatcttttgt ttccggactt
    12181 cgcgaaaata tggtgccagg aacagcaact gcagcacttc aagtaccaag tgcagatgca
    12241 gctatcgcga tggaagaagc attttaccaa ttcatcgaag caaatccagt aagcggaacg
    12301 attgaagcgg acaatacgta tgtaaaaatc gaattagttg gtaaaggcgc acatggtgca
    12361 agcccacaat ctggtatcaa tgcaggttct ttcttggcat tgttcttaga taactatgaa
    12421 ttcctaggat ctgctaaaca atttatccat gtagcagctg cttatgtcca tgaagatttt
    12481 tatggagaaa aactaggagt cgcttatgaa gatgaaaaaa tgggcaaatt gacaatgaat
    12541 gctggactat ttgcttttga agaaaatggt acagaggaag cgaacttcat caatatgaat
    12601 ttccgttttc caaaaggtgt aacagtagac ggattgcaaa gtgacatcga acaaacagtg
    12661 gggcaagaag gcgcaacagt tactcgaggt gctcgcgtaa tggaaccaca ttacgtacca
    12721 atggatgatc cattggtggc tacattattg caagtctatg aagaccacac tggagaaaaa
    12781 ggctacgaac aaatcatcgg tggcggaact tacggacgtt tgttgaaacg tggcgttgct
    12841 tatggagcga tgttcccagg atatactgat acaatgcatc aagcaaacga atttatgagt
    12901 cttgatgact tgttcagagc aacagcaatc tatgcagatg ccatctatcg tttggcgaaa
    12961 taattaatta aaaagaggat caagaattgc aaattatttt tgcagtcttg atcctttttt
    13021 tactttctac tgtggtattc atgataaaca tgatatagat tcaattcata ttctttctta
    13081 gcgttcgtga cgactgtatc caaaatcaac cctgttacta atgaaagaag tgctaaagtc
    13141 atcaatccag tacttaaaat cgcagaaggg atgcgtgtaa taaagcttgt cagcatatat
    13201 tcgcgaatca caggaatccc cgtgaccaat ccaaataaaa agaagaagat ggtccaaatc
    13261 ccaaagaaaa gcaaaggctt ataatcttta aacattttaa taatcatcat gatcactttg
    13321 aatccgtcag agaatgtatt caattttgat tcgctgcctt caggacgatc acgataatcg
    13381 attgggattt caacgaactt gaatttttta tctaaagcat ggatcgtcaa ttccgtttcg
    13441 atttgaaaac cagaactcat aataggaaaa cttttgacaa agatacgatt gaatcctcga
    13501 tagccggtca tgacatcaag aatttttgtt ttatacattc tagtaattgt atttctaaca
    13561 aggttatttc caaaatcgtg gaaaggtctc ttattctcat caaagtaggt tccatttgat
    13621 aggcgatcac caatgaccat atctgcttca ccagaacgta atttctcgag caaaccatgt
    13681 acagcttctg caggataagt atcatcgcca tctaccatta aataatagtc ggcatcgata
    13741 tcaaagaaca tttggcggat cacatttcct tttccttgac gaggttcttt ttttacgatt
    13801 gctccacctt gaacggccaa ttcgtatgtt cgatcggtcg aattattatc atagacataa
    13861 atatctgctt ctggtaattc tcgtttaaag tctgcaatga ctgttgaaat tgttgcttcc
    13921 tcattgtaac aaggaatcag aacagcaatt ttttcttgtt gttccatttt tcactctcct
    13981 taactataga aaacaaaagc taaattaaaa atacctcata atcaaaggaa atgctagttt
    14041 ttcccgctaa atatcattta tttaaaaaga aaatactgga atatatgttt ggaaagctct
    14101 atctaaaaaa ccacccacta caaaagaagg acatgatacc tctcttttga atagtgggtg
    14161 cgtattcgtt ctctcacttt tttgacgtat taatctgttg cacctttttt tcttgaaatt
    14221 tctaatacgg ttcctgtatg agcatctgct aaaaattcat aataaacagt ttcgccatct
    14281 tctattcgag aaagacctcc ccgataagca ctcgaccgaa cggcgaattt cctaataggt
    14341 tccttttcaa aagagatcca agaaccttcg attgcacctt cttttagaaa agatgacttg
    14401 atattttcaa gtacttggtc tgctgacaat acttttttgc gatgatacca aatcgttgaa
    14461 gctaccccgc tcaataatcc tagaccgatt ccaactgcta acccgccttt aaaatagctt
    14521 agttcgcttt cttcatacat gtggacatac ctccttcttc tcacttgtta ctccttgtgc
    14581 ttattttacc acactctcat attttctttg aaataaataa ccgctaccag aaaaaataag
    14641 ctataataaa tacgatcggt ctgcagaaaa aatcatattt tatccgcaga tcaatataag
    14701 tctgagagga gtttagtctg aaatttagac gaatagcata tggaagaaaa aacatttcaa
    14761 cgaatcaaag agttaacaga attacaagga acaagcggtt ttgaacaaga tgttcgcgct
    14821 tatatggaaa gtcatatgga accgctggta gatgagttac agttagatgg tttgggcggc
    14881 atttttggtc tgcgccatca tgaagaagcg gatgcacccc gtgtaatggt cgcagcacat
    14941 atggatgaag tcggattcat gctgacacaa atccaagaaa acgggctttt taaagtcgta
    15001 cctctaggcg gctggaatcc ttacgttgtt tctgctcagc gttttacact taaaacctct
    15061 actggtaaga attatccatg tatttcttct tccgttcccc cacatttatt gcgaggaaca
    15121 agcggacaaa aatcagtaga agtaaccgat attttgttcg atgctgggtt tgaatcaaga
    15181 gaagaggcaa tgagttacgg tgtccttcca ggagacacaa tcgttcctta cgctgaaacg
    15241 atcaaaacag ccaacggaaa gaatattatc agtaaatcat gggacaaccg ctatggctgt
    15301 acaatggtat tagaagcttt agaagcgttg caaaatgaaa cattaggtca tacattgatt
    15361 gcaggcgcga atgtccagga agaagtaggc ttgcgtggtt ctaaagcttc tgtcaataaa
    15421 ttcaagccag atctgttctt tgcagtagac tgctctgcag cagatgatac agtgacgaaa
    15481 aatggcacat tcggacattt aggagaaggt acattgatgc gtatccaaga tccaggtttg
    15541 atcatgcttc ctcgtttgcg cgagtacttg ctggatatcg ctgaaacaaa caatatccct
    15601 tatcaatact ttgtttcaaa aggcggtaca gatgcaggtg ctgctcatac acaaaacgaa
    15661 ggaatcccaa gtacagtgat tggcgtagtg ggacgttata tccatacgca tcaaacgatg
    15721 ttcagtattc gcgattttga agctgcacga gaaatgttga ttcaaacact gaaaggtttg
    15781 gataaatcaa cagtgaatac gatcgtatac ggaaaataat gcgatagtga aactataaaa
    15841 tctggcaaaa gattgctttt tgccagattt tttttataaa aagaggatca atgtttgtta
    15901 gttcgaaaga aaggtgtata atttaattga aaaatacata aacagaaagt agaggacaaa
    15961 gaatgattat cccaaatagt ttagaagaat tagcaggcta cgtagaaact ggaaagagtg
    16021 tatttttctt tactgccggc tggtgcggag actgccgttt tatcaaaccg caaatgcctg
    16081 aaattgaaaa tgacttttct gattggcaat ttattgaagt agaccgtgac aagtacatag
    16141 atgtagccgc tgaatggaac atttttggta tcccaagttt tgtcgtgatc caagacggaa
    16201 aagaattagg tcgtctagtg aataaagatc gtaaaacaaa acaggaaatc gaatcgttcc
    16261 tagaaagtgt aacaaaaggc tgacatctgt taggaggggt caagatgaat caagaatata
    16321 agaatatttt agtaggtatc gacggtagtg aacaagcact tgctgctttc aaaaaagcgg
    16381 tagaagtggc tcgccgtaat aatgggaaag tatatgtagc aaacgtaatc gatcagcaat
    16441 tttataactt tatgggctat gcacctattg atcaaaatct agtcgatcag caaactgaaa
    16501 cagccaagga aatgatcgaa gaatgtaaag aatatgggaa atctgttgat tacacaaata
    16561 tagaaggaat tattgcatat ggttctgttc gtgaatccat ggcagtcaaa ttgccaaaaa
    16621 aatatgagat cgatctaatc atggttgggc agtcaggtct aaatgcagtc gaacgcttta
    16681 tgacaggaag tgtagccagc tacgtgatac gtcattcacc ttgtgatgtc ttgatcgttt
    16741 ctgacgaaga agaacagcca agtaattaag caagtggaac ttgtacgacc tttttaatca
    16801 attaactaaa acagtaaagg gactgtgaca aaagtcttgt cacagtccct tgttacgaat
    16861 aaatggtgtt ccagaagagg cttcttcgga aataaagcat gaaaaaaatt tcatgcagtg
    16921 aatgcaattt tttgttaaga taaatctcag caaaggataa tggaggaaag aacatgattt
    16981 ttgcttataa caaagcacac gtcggagaca ccttgatggt tattatagcc gacgataaag
    17041 gaacagaaaa cactgttgaa cgcaaatgga atgttgctcg gatcaaagat gaacaaggtc
    17101 aagttgttgc ttggaacttt ttccatatct ccgatcatct aacaatcgaa gggaacggcc
    17161 aagtaagctt aacagatgag gaattagcga aattgaatcg attgatcaaa gaagcaggat
    17221 tcgaagaaca gcttgaagct gatcatgaac cgaagatcgt tgtcgggtac gtaaaaacat
    17281 gcgtcccaca tcctgattct gaccatttat cgattactga gacagaagta gacaatggta
    17341 cagtattgca aatcgtttgc ggtgcaccca atatcgaagc tggacaaaaa gttgtcgttg
    17401 caaaaccagg agctatgatg ccagatggaa tgatgatctg gccaggaact ttgcgagggg
    17461 tagatagcta tggaatgatc tgttccgctc gtgaattgca tttgccaaat gctccagcaa
    17521 aaaaaggaat cttagtattg cctgaggatg ccgtagtcgg agaagcattt ccaaaataaa
    17581 gaggttgtga caagtctttg tcacaacctc ttttttatta attttcacta ttttgatttt
    17641 gttttaatgc cgattgatcg atcgtcaatt caacagtagt tgttttttct ttcgagcctt
    17701 catagtaagt gattttgaca gaatctccca cttttttctt atacaaagca gattgaagtt
    17761 ctacaccaga agaaacttct ttgtcatcga tcttagtaat gacgtcgtat tgtttcaatc
    17821 cagctttttc agccggtgta gctgtttgga catttgtcac gatgacaccg ttagttacag
    17881 atgaaggaat cttcaagatt tgttcttgtt gctgagtaga aacggcagat aagtcgacca
    17941 ttgtgatacc tagagctgga cgtgtcactt ttccgtcttt ttccagttga ttgatgatat
    18001 tcactacatc gttacttgga atagcaaatc ccattccttc tacgcttaca ttagaagtag
    18061 attcagaagt acttgcgatc ttacttgagt tgataccgat aacttgtcct tcgatattaa
    18121 ctaaaggacc accagaattt ccagggttga ttgctgcatc tgtttggatc gcattgatat
    18181 tgactgtttc gttcgattca ttcgtacttg tcacttgtcg gttcaaggaa gaaatgattc
    18241 ctgaagtgac agaatttgca tattcagatc ctagtgggga accaatcgca atcgcaggct
    18301 caccaacctt cagagcactt gagtcaccaa aagaggcaac tgtttcgact ttgtctgaat
    18361 tgatcttcaa caccgctaaa tctgaataag catctgttcc aactaattca gcttttacct
    18421 tcgtaccgtc tttcatcaag acttcaagac cttgctggcc atctactacg tgattattcg
    18481 tgactacata agcagtattc ccgtcttttt tgtagatcac gccgcttcct tcactataag
    18541 cttccaaatt actatcatct gagctgctac tttcttcttg ctgcccgaat agtccgccaa
    18601 aaccgcttga ttggttttga ctttgtaagt tgataacaga aacgacagcg tcttgaactt
    18661 tatctaccgc tttggtgata tcagaatcta cattgacttt gacattttcc acaactgttt
    18721 caccggcaga attctgattg ccgcttgtag cagtagaact attgttgcca gtgcccatcg
    18781 ctgcataaaa aatgccagct gtcagtagtc ctccaacgat ccctccaaca agccccagac
    18841 ctaacttacg ccagaggcta ttatttttac tttttttcat ctttggtgtc acattttttc
    18901 tatccatgtg ttcctcctcc aattacattc atttatctac tgttagtata gtcttatttg
    18961 ttgactttag tctactttga gactgtgaac tttttatgaa aaactttagg aaaaatcatt
    19021 gttttcagat gaaaatattt gattttcttt ttccacagat actgtgtaaa actacgcgga
    19081 taggattgga aaaataaggg ataaaacata tccacagcct gtgtataaag tggataaatt
    19141 cttataaaac gtatgtttgc ctaatgaatg tgatgaaata aggtaaaata acactgtttt
    19201 ctgtggataa acctgtggat gttgtgcata actgttgggg aaatcttttt aacaaaaata
    19261 gccaaaataa acatatagac gaaaaaattg attataataa acaaagtgag catgaagacc
    19321 aaaggagaga aaacatgaat atcaaaatta tctcagtagg aaaattaaaa gaaaaatacc
    19381 tgatccaagg tatcaatgag tatgtcaaac ggctgaatgc ctatgcaaaa atcgaattga
    19441 tcgaagttcc tgacgaaaaa gccccggaga atttaagcga agcgcaaatg agacaagtaa
    19501 aagaaaaaga aggggaacgg atcttagcga aaatcaaaga acaagaatac gtctatgcct
    19561 tagcaattga agggaaaaat cctacaagtg aatcttttgc taaacaaatc gaccaattag
    19621 gtatccaagg aaaaagccac cttgtttttg ttatcggcgg ctctttagga ctcagcgaag
    19681 ccgtcatgaa aagaagcaat gctcagatct cttttggaaa aatgacgtat ccacatcaat
    19741 tgatgcgtct gatccttgtc gagcaaatct accgtgcctt ccggatcaat gcggg
//
LOCUS       DANKHL010000034        19160 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000034 DANKHL010000000
VERSION     DANKHL010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.036"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(58..783)
                     /locus_tag="PW101_000849"
     CDS             complement(58..783)
                     /locus_tag="PW101_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDL2513271.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(1144..1214)
                     /locus_tag="PW101_000850"
     tRNA            complement(1144..1214)
                     /locus_tag="PW101_000850"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1180..1182),aa:Gly,seq:ccc)
     gene            1331..2083
                     /locus_tag="PW101_000851"
     CDS             1331..2083
                     /locus_tag="PW101_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL2513272.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNNLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            2099..2578
                     /locus_tag="PW101_000852"
     CDS             2099..2578
                     /locus_tag="PW101_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL2513273.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(2829..4409)
                     /locus_tag="PW101_000853"
     CDS             complement(2829..4409)
                     /locus_tag="PW101_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HDL2513274.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(4402..5145)
                     /locus_tag="PW101_000854"
     CDS             complement(4402..5145)
                     /locus_tag="PW101_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2513275.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(5321..6097)
                     /locus_tag="PW101_000855"
     CDS             complement(5321..6097)
                     /locus_tag="PW101_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2513276.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(6345..7181)
                     /locus_tag="PW101_000856"
     CDS             complement(6345..7181)
                     /locus_tag="PW101_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HDL2513277.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(7192..8121)
                     /locus_tag="PW101_000857"
     CDS             complement(7192..8121)
                     /locus_tag="PW101_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDL2513278.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            8313..8666
                     /locus_tag="PW101_000858"
     CDS             8313..8666
                     /locus_tag="PW101_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL2513279.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(8740..9396)
                     /locus_tag="PW101_000859"
     CDS             complement(8740..9396)
                     /locus_tag="PW101_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL2513280.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            9622..11556
                     /locus_tag="PW101_000860"
     CDS             9622..11556
                     /locus_tag="PW101_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2513281.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(11642..12865)
                     /locus_tag="PW101_000861"
     CDS             complement(11642..12865)
                     /locus_tag="PW101_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2513282.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(12859..14757)
                     /locus_tag="PW101_000862"
     CDS             complement(12859..14757)
                     /locus_tag="PW101_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HDL2513283.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(14751..15869)
                     /locus_tag="PW101_000863"
     CDS             complement(14751..15869)
                     /locus_tag="PW101_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2513284.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(15952..16785)
                     /locus_tag="PW101_000864"
     CDS             complement(15952..16785)
                     /locus_tag="PW101_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2513285.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(16778..17695)
                     /locus_tag="PW101_000865"
     CDS             complement(16778..17695)
                     /locus_tag="PW101_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2513286.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(17718..17897)
                     /locus_tag="PW101_000866"
     CDS             complement(17718..17897)
                     /locus_tag="PW101_000866"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513287.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(17922..19046)
                     /locus_tag="PW101_000867"
     CDS             complement(17922..19046)
                     /locus_tag="PW101_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2513288.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         5973 a         3773 c         3198 g         6216 t
ORIGIN      
        1 atgtatgttt ttacacaatt ttttcataat attcataaaa aattcgaata gctgctacta
       61 attttttgtt tttgttgctt gtcctgtctc ataattgatc gtgtagccat cttgaacatt
      121 gtagataaag acattgaatt caattttttt gtcttcaatg ctttctgctt ccaattgcac
      181 tcccctggct accagttcat tcccctcaaa atgaggagtg acacggtagc gtacatgatg
      241 atttgtttct ttgatatatg ccgcaatatc attttcgtga gcaagcatgt atggagcatt
      301 caacgaacgt gtccctgtca tcaagttttt aatgttgtta ttttgaccag tcagctggaa
      361 accaattaaa tggctgcgat tatataacca ttgcccgtca cttaatttct tttgtttcca
      421 acctgtcggg tcaatataca aaggctctct cttttcagta gggaacaaat cttttccaag
      481 catagcattg gcttgtccta ctctgttcaa tttatcaata tttgaatagc tttcccatgt
      541 tcctttggaa aggctaagat cctcttttgt aaattccggc tgattcccat tgatttctat
      601 ttcttgatgt tttccatcat attctggcac atccgctact tgagaagctt gtggtgtgga
      661 atcaaaaata gttgtaatct gctgaaagat atctgaatct ttttgtgtgg tggaaacacc
      721 gaatgcccct gcaatcacag cagcgacgat ccccacgatc gccttggttg tttttttgct
      781 catttcttta ctcctttact aacagctccc taaatcttaa catatgttcc cctataaata
      841 aatactagaa agaaatagaa acagcaaaaa ataaaacctg ttttaagatg gcttaaaaat
      901 acaaaaaaga gacggattcc tcgtctctaa tcagtcacca ctctatctgt aaggtattta
      961 ttattattca aaaattttct tcatactcaa gacaatacac tgatttcaca accgcttggt
     1021 aaacggtgcg caaaagcttg catctgcggt aataagtcca tccaagttaa aaacataaga
     1081 agttgttttt gcttggctgt tcttattact tgttgcagag cagctttttc acaacctatt
     1141 ttaagcgggt gacgggaatc gaacccgcga catcagcttg ggaagctggg attttaccac
     1201 taaactacac ccgcaataca aaaacagtat acgcctcttt tattccttcc tcaagttttt
     1261 ttattcttta cgcttctggt atgttttgta gtaaaataaa actaatcaat caattattgg
     1321 agaagatgct atgacgggaa aagaattaga tctgttgctg gacacttgtt tactcgctgg
     1381 gaaaatcatg atggaaagca atgcggagat gtatcgggta gaagatacga tgagtcgaat
     1441 tgctttagct tctggaaatt tccgtttggt cagctatgtc actcaaaccg gtctctttat
     1501 tggtcttgat cgtacctcga ccatccgaat ggaacaaatt accaaccgtt cgattaattt
     1561 agaaaaagta gtaaaagtaa ataatctttc acgtaaatat gttgcttcag agctaacatt
     1621 agaagaactt tactgcgaac tgcaagcaat cgaccgagac cgttctttct ttcctgtctg
     1681 gttgcaaatc gttagcgcgg cagtgatcag tggaacaatt atggtgttat ttggcggaaa
     1741 gatgaccgat ttacctgtga ctttagtcat tggaggattg ggatacgcag cttatctata
     1801 tagtttgaaa ttttttagaa tcaaattttt atcggaattt ttatcttctt tattgattgg
     1861 catagccgct atctacagcg tacgttttgg cttaggaagc aatcaggatc taatcattat
     1921 cggatgtgtg atgcctttag ttcctggagt tcaaattacg aacgctatcc gtgatttgct
     1981 agctggacat tatgtctcag gagtttctcg cggaacagaa gcgatgatga ctgctacgat
     2041 gatcggtttt gcgattgctt ttattttcca gcttttttat taggaaagga acacaagtat
     2101 ggggacgata tttattcagt tttcttttag ttttttagcg actgcggcat ttgccgttat
     2161 tacaaatgta cctaggcgtt cattgatttg ttgtggtctt tctggtgctt ttggctggat
     2221 gatctactgg atctgtgttc aaataggcgg atctgctgct tttggttcat tgcttggttc
     2281 tttaggagta gcattcatca gcgatctttt ctctaaacgt ttgaaaatgc ccgtaacgat
     2341 tttcaatatt cccgggatgg ttcctctagt ccctggtggt ttagcttatc aagcggttcg
     2401 aaatctcgtt actgaatcct atcgagaagc ggctcattat gcggtccaag caattatgat
     2461 tgctggagca atcgctttag gtcttgtatt atcagaagtc tttaaccata acatccgtaa
     2521 tttcaaagta aagcgtgacg caatcatgat gaaacgaaaa aaacggcttt ttaaataaac
     2581 gaagtgtaac aaagaaaagg ttgtgaaaga gacgttcagc cttgagcgat gacaaaatat
     2641 ctcacaaaca ttgcgacaag taaccgcagg agcaataaga agtaattatg agtattaaaa
     2701 aaagttttgt aaaaatagct tctcacattt ttacataaaa ttgacttaat actgagaaac
     2761 tgacttttga acaccctcta tctgtaaaaa aggggctgcg acatgatttt tgtcacggcc
     2821 cctcttcttt attcaaattt tttccaaaac gttcgttgat aataaacgat ccatagagcg
     2881 gaaagtatta gaactaaacc gatcacagga atattcacta accaaaatgg ataaaacaaa
     2941 gccaacattc cgtataagac gataagaata atactgatga tcaatgaacc catcatagtt
     3001 gcgactaaac ccaaacttcc tgaacctctg ttaaataatt ggttaatatt ggtccagttc
     3061 agcagcagca accgataatc tctagcgaaa taacgtagtg acaataagta gcagccgaat
     3121 aaagaaccaa atagcaagct agggataaaa aacaaaggca gctgaaaaga cagtccgctt
     3181 aataatgcga tcccaccagt taaaaaactc tgcaatatcc atccgaatct gaacttttct
     3241 tttaaatatt ggttcataga gattggcaaa gaacggataa acagaaaatt ttcttgatcc
     3301 aaagaaataa gattgctgat aaaagaagtc tgattaactg ttaagaaagc caatgcgatc
     3361 cctaccagga aaacaacacc gattaactta ttatctaaat cagttaattt tatttcgcct
     3421 cctagggcaa aagcaagaat aaaaatgatt ggagttaaca aggaagaaga aagaacttgc
     3481 ataatcaaat taggttcttt tactaattga gcattgtaac taaataaaag ctgatgcaca
     3541 ttttggttct ttttatgggt tctttttatt ttgaactcac tgtttgaatc agtcagttgc
     3601 tctgtaagct ttggtactat cagctgtttt acagcaagga aaaatcccag agccgtgata
     3661 gctagtattc cccaacccaa taaaccttgt tgagaaagtg gctgactggc agcatcatag
     3721 aatggcaata atatcgaaat cggatgccga tctgttaact cattttcaat agtccccgtt
     3781 tgatgattca tccacatgat tccagcaaat gcgatcagca tcgagctgcc aagcaagata
     3841 ctagaaactg tttttctatg ttttttaaac actgctgttc gagccaaacc aaaaacaatc
     3901 aaactagcca gacaaaaaag aataatcaaa aatagagaaa acaaaaggat tgagataatg
     3961 atagtaagca aaatccctag atcagctcgc catccggtta aaataaacat gactagaagc
     4021 ggaaaaacaa aaggaatgat ggtcaaagca actaccaaaa tctttgctga aaaaacctgt
     4081 tcttgtccaa aaggcagagg caaaaatact tgcaggtcat ttccttcaaa aaagatatta
     4141 taaatgactg atatactttg agttaaacct aacataccaa aaagtgccac gtaataagta
     4201 aagaaacctg gcaatttaac aaagttcata gcaaacatag caaatccgta aatcagtaaa
     4261 aaaatcagcc ctgaaaaaag attttgagac aaaagataga tagttaatcg ctttcccttt
     4321 tttcctttct ctcttgcttt attcgtgagc tgtggattaa catataaaag attaactcga
     4381 attaattcta ataattgtcg cttatacata cggtccatct cctgtcgctt gccttccagc
     4441 cattttcaaa taaatctttt ctagcgtttc atcaggtgag ccattcaata attcactaac
     4501 tgccccctga taaatcaatt cacctttctt caaaatagcc agtttatcgc ataactgttg
     4561 tgcggtatcc aatacatgtg tagaaaaaat gactgttttt ccttttgcgg catgagcctt
     4621 catcatttct ttcaaatcaa atgctgcttg aggatcaagt ccttgcaaag gttcatccaa
     4681 tacccaaata tctggatctg gcagcaacgt gccgatcaaa atcgtttttt gacgcattcc
     4741 atgtgaaaaa cttgcgattg tttcattttg atgactatac atatcaaaaa gcgctgtcaa
     4801 ttctgctaaa cgcttttctt ttttttgtgt gtctaattca taggcagctg aaatcaagtc
     4861 ccaatattct ccagccgtca attgtaaaaa aatatctggg gaatcaggca cataaccgat
     4921 tttttgtttg atactcaatc gattttccgt caatttcatt ccatctacta aaatcgttcc
     4981 cgatgatggt tcaataatac taactaaact tttaatcgta gttgattttc cggcaccatt
     5041 atgtcctaaa aatccaaaaa tttccccttg tcttatcgag agagacaagt cttttaatgc
     5101 ttgttttttc ccgtaacttt tacttacatt gatgaattca atcatttctg tactcctttt
     5161 gtttgttatt actttttatt gtacccttcg ttattggtgg agtaaatgtc aaaaagagtg
     5221 cataacaaaa aaggatagaa acgactaggc tgctacttgg gttgttctct tctacaggaa
     5281 aacactcttc taacaggtaa gatatgagtc tttgttttct ttaaaatgat tcattttcta
     5341 tcttttcttg cataaaattc tttagtttat caatacttct ctttcccatc tctagttgtt
     5401 ctccatttgc aaatgttact atttcgttta ctctgctaat agatgtaatg ttttcagtat
     5461 taatgatata agcttttaaa tctttacata aatactcata ttcgagtgat tttttatatt
     5521 ttttaattgt gccgtttacg agaatctctt catctttcaa tactaggcct attttatttt
     5581 tataacctgg catgctcatt agataaagga tttcatcgta atggatatag tgatcgctat
     5641 taccataacg gacagttgtc cacctttttt tcgtagcatg ttgaacagga tagttttctt
     5701 tttctttcaa taagtaactt tggatgcttt ttttattttt ttcataagtg aattctttta
     5761 tcaaataacc actaggcgta atctttcgat taataacagt tattgcttta ttcggatgag
     5821 acgttagata aacaatttta cacttttcat tcttttctct aagcatttcg cctagttcga
     5881 taccgctata ataggcgttc aaatcgatgt ctaggaaaaa aatatctgag gaatagattt
     5941 cattattttt aatgaattca acaaattgta taacgttcaa tccctgaacg atctcaaatt
     6001 tccaaggact taataaaatt ctggattctt ccaaaatttc tactagcatt ttttcaaaaa
     6061 ccaaactatc atccaaaata aatatacgcg ttaccataaa aagtacctcc actcgatatt
     6121 ttactatcaa aatcatttca tacaactttt caacatacat gttaggaata aattgtaatt
     6181 ctcttcacaa tcttttccaa cacctttgaa accgatatca aatactttca ggatgctgct
     6241 tctaaaataa atatcaaaca aatgaataca tcatgcaaaa aaagccagac aaccgacgtc
     6301 cttgcgtgtc agctgtctga gctatgttta tttattttgt tcttttaaaa agaacgatac
     6361 acatactgat ttttcggagc agagactttc tcagcgtttg taacaactac agatgggatt
     6421 tcacgcttga atggttgata atagtctgat tggatggttc cttctatttt ataccattca
     6481 tcatttttaa ccgttactcc ttccggcata tgcgtcaaca aaccaaagac tcctgaatct
     6541 gccacacaat ggataatgcc gaatcggaac aagaagatat ctgtattgct atcgttcggt
     6601 gtttggtaaa caaatccttc atagctgatt tttttgccaa tgaattcgct agggtaatta
     6661 taaattagct ccataacttc taaatagttt tcatctgtga tcttgatagt ttgcttatcc
     6721 ccatactttt ctttcaactc attcatttgg tctaagtagt ctgatttgtt gaaataaata
     6781 ctggtatccg gctttaagta ttgtgtctgc atctccggat cccctactga ttctttactt
     6841 actgggaaat taaatccttt ggcttctacg atcgtagtgt cgagactaac tgttggaaac
     6901 aacgtcccca cgattactgg caaagctagc agtacataag cgattccacg ttgataaggt
     6961 tttgacaacc catggtcatg atcgtgctcg tgtgcctttt cttctgcttt tccgtttttg
     7021 acccacaaat acagctgaac taaagccaaa agagcagata aaatcattga aagaatagct
     7081 aagtagcgat aatggacatt gatatattga tcgagtttgc cagaaacttg caagtacatc
     7141 atcaattcga aatacccaga taaaatcaaa aaccgcagca tattttccct cctaaataaa
     7201 taatgtgaat ccaataacgc taagggcaat cagtccgatc agcgataaaa taaagcgtcc
     7261 tgtaaaatat cgtttcatca tcagtagatt tttgatatcg accattgggc caaagaccaa
     7321 aaaggcagct acaggtgctg tgccgaacag acttaacaaa gaagatccta taaacgcatc
     7381 tgcttctgag caaagagaca tcgtaaaggc caataacagc atgatgataa tggctaaaat
     7441 ctttgttgag ccgagcgtta gcataattct agttggtaaa taagtctgca tcgcagctgc
     7501 gattaatccg ccaatgatca aataacgacc tgtatcaaaa aattcgtcga ttgaatgtgt
     7561 caacacatgc ccagtttgat ggatcagctg attttttggt tcattcttct cactttgttc
     7621 ttcttcatgg acatgatgat ggtgattttc tgctacttta gacgtgattt tttctttcaa
     7681 tacagcgtct ttttgaaaat aggcaagcca gacaccaatc acaagggcaa caaaaaagct
     7741 gcctatcacc cgccagcttg ccatctgcca agaattgcca aatgcgataa aagtggaaaa
     7801 aatcacgatt ggattgatga tcggagcagt cagcataaaa gcaaacgctg tgtgaactgg
     7861 tacgcctttt ttaacaaatt gatgaacgat cggtacgatg ccgcattcac atgaaggaaa
     7921 gaaaaatcct aagatcgaac cagtaaaaat agagaagata cgattttcag gaagccatct
     7981 cttcacacgt tctggggtca aaaatacttg taaggcgcct gagataagac agcctaataa
     8041 tacgaaaggt aatgcttcaa taacgatcga taagaaaatc gtactcatct gcaaaaccga
     8101 atttggaata aaagaaaaca tgagtttcct cctttttatt tctagttcat tcgctttgca
     8161 aatatacgct tttattcaaa aaaaacaagt tgctctgtaa atttttacac aactcttatt
     8221 ttttaccaaa gcacttcaat tgactttttc tgttattccc attaaattaa ggtctcatac
     8281 atatcacttc tatcataaaa gggagaataa aaatgaaaca aaccactaac ttgactgaag
     8341 ttcaagacat actgcaacag tcatccgttg ccgtcattta tctttcgatg cctaattgca
     8401 gtgtttgtca cgctgttcgt ccacgactag aggaacttct cacacattat gatattccgg
     8461 cacttcattt ggatgcattt gaaacaccag aagtagctag taaatttgaa gtgctgactg
     8521 cgccagttgt cctgattttt catcaaggaa aagaagtctt tcggcaagca cggttcatcg
     8581 actttaaaaa aatccgttat ctactagatg aactgacagc agaagatgat tccctttctt
     8641 atgaagaaat cttccgaaca aaataatcgt tgtccaaaca gaaaaaagcg attgaacaag
     8701 tgaaagtttt gccttcattt gttcaatcgc tttttatttt tattctgctg tattttcgga
     8761 atgcgcattt gcgtcagaat gcaagaaata gatcaaggtt tgcaattcat tcgtcaaatc
     8821 aacattttgg atcaatacat ctgacggagc attcaatcga gcagcggtaa agttcaagat
     8881 tcctttgatt cctgcttccg ttaactgatt gactacttct tgtgctcttt cagcgggtat
     8941 cgttaaaatt gctacttcga tttgttggat cttgatttgt tctgacatat cttccattgg
     9001 ataaacgggg ataccgtcta cgatgcgccc aacgatttcc tcattcacat caaaagcaca
     9061 actgatacgt atgctattac tttgatggaa tttgtatttt agtaaagcac ttcccaagtt
     9121 ccctacaccg atcagcgcaa catttgtcaa ttgatcgtcg tttaatgtct ttgcaaaaaa
     9181 gtgcataaga tcttctacat cataaccata cccacgtttt cctaattcgc caaaataaga
     9241 aaaatctcga cggatcgttg cactatctac ttgaacggct tcacttaatt cagttgaaga
     9301 cacttttttc tttccagcat tatttaaaat ccttaaatag cgataataca aaggcagtct
     9361 tttagctgtc gcctttggaa taatatgatc tttcacgaaa tgacctccac gtattgtcta
     9421 attacatctt tgatttctcc atcatagcag tatttatagg aaaaaatcaa ttccattgct
     9481 cagatattgt gaaattttat tctttattca tttatttatc atttctgcaa atctcgcagt
     9541 ataatggaat ttatggtaga ctactaattg aaaaatagtt ttttatttga ggagaattca
     9601 caaagtagaa aagaggattc catgatatta ttacaagcca atcagattgc tcgccatttt
     9661 ggtgcagaag tcttatttga aaacatacat ttagaaatca gtacaggcgc tcgtgtcgct
     9721 ttagttggca gaaatggcgc tggaaaatcg actttattaa agatcattgc tggaattgaa
     9781 gcacctgatg aaggaaagat cgctaaaaat aaaacagcga ctctcggata cctcgctcag
     9841 gacacaggac ttgattccaa tgaaacagtt tggaacgaga tgctgaaagc ttttgaacaa
     9901 gttagacaaa tggaagagcg gatgcgcgag ctagagattt tcattagcga aggtacgcca
     9961 gacacacctg cttatagttc ttatttaaaa gaatacgacc ggctgcaaca cgatttttca
    10021 gatcgaaacg gctatggtta tgaaaatgaa attcgttccg tattgcatgg gtttaaattc
    10081 gattcttcat tttatgatca gaaaatcgat actttatcag gcggacagcg cacacgttta
    10141 gctttagctc gcatgctttt gcaacaccct gacattttga ttctagatga accaacgaac
    10201 catttagata tcgagacact ttcctggctt gagaattatc tgcaaagcta tactggtgca
    10261 ttattgattg tttcccatga ccgttatttt ttagataaag tagtcaatga agtctatgaa
    10321 ttaagcagaa gaaaaatgcg ccattacaaa ggtaactatt cgaaatatct agatttgaaa
    10381 gcagaacagt tagcaagtga atggaaggct tatgaaaagc agcaaacaga aatccataaa
    10441 ttagaagact tcgttgctcg gaaccttgtt cgcgcctcta ccacaaaacg agcgcaaagc
    10501 cgcagaaaga cattagaaaa aatggaccgg ctggatcgtc ctcaagggga tgaaaaacaa
    10561 gcaaactttc tattcagtat agaaaagcct tctggaaatg tagtcttgca aatcgaagat
    10621 gccgctatcg gatatgataa tgtcacctta tctgaaccaa tcaatctaga tatccgtcgt
    10681 caagaagcga ttgctctagt aggtccgaat ggtatcggga aatcgacgtt gctgaaatca
    10741 atcattcacc agatcccttt tatccgtgga aaagaaacgc tagggacaaa tgtcaccatc
    10801 ggctattacg atcaaggtca agctgatctg catacaaaca agacgatcct agcagaattg
    10861 tgggatgagc atcctaccac tccagaaaaa gagatccgaa gtgctttagg tgctttctta
    10921 ttttctggcg aagatgtaga aaaaccaatc cctctgctaa gtggcggtga aaaagcacgg
    10981 gttgctttag ccaagttagc gatgaacaaa gaaaattttt tgattttgga tgaaccaaca
    11041 aaccatttag atattgacaa caaagaagta ttagaaaatg cattgatcga ttatgacggc
    11101 acactgcttt ttgtttccca tgaccgatat ttcatcaatc ggatcgcgac aaaagtcgtc
    11161 gaactctctg aaaatggcag caaagtctat ctaggagact atgattatta tttagagaaa
    11221 aagcaggaag aagaagaaat cgccgaattg ttggcacaag atgagccaga atctgctcct
    11281 gctcccacga acaaatctga ttattaccaa agcaaagaac agcaaaagct tatccgtagc
    11341 cttcaacgaa aaattactca ggttgaagaa gaaatggcgc ggatcgatga atggatcgat
    11401 cagttggaac aagaaatggt cgcgccagaa aacctggaag atcacgtaaa actcaatgag
    11461 ctgaaccaag atttagaagc tgctagacaa gatcaagaaa caaaattgac ggaatgggaa
    11521 gaactaagct tggaattaga agaatatgaa aattaacgaa aaaaccaaaa cagaaaccat
    11581 tcatgctgtt ttggtttttc tttttttatt ttatcaaaca aaaattccat atttaccttt
    11641 ttcaccaata agaagaccaa ttactagtca ttctcactaa agcttctaaa taatagtaat
    11701 caccccaaat acaaggtgta tttaccccat tatttttggg cttattataa acagactgtg
    11761 ttaaaaagcc atcttcttct tccgttgtag catatctttc tttcaattgt cgcataattt
    11821 tccaaacagc agactggtat aagttctttt ctgttttgtc taatagacct gattctatta
    11881 attctaataa tccacaaacc atgatcacca tactagatgt atctttttct tctctgctag
    11941 aatcatcaaa gatcaaatcc caataacaaa tggtatcttc tggcaaccga ttcataaaat
    12001 aattggctaa tattttgctt aaatcaaaat atttttgttc tttcaaataa cgataactta
    12061 atgcaaaacc gtaaatcccc catgcttggc ctctagccca acaggaatta tcactataac
    12121 cttgatgcgt acttccatat cttggttttc catctttgac atccatataa aatgtatgat
    12181 aagttgaacc atcttctctt actaaataat ctgctgcatt ttgaatatga gcctgagcag
    12241 cttttttata tttttgattt ccagtttctt cactcgccca atacaataat ggaagattca
    12301 tgcaacaatc catgatcatt ctaccttgtt gtgaaggatc actaggatct ccccaagctt
    12361 gtataactcc tgccttctcc caaaaacgct gaagtaatgt ttctgcagca tcaattgaag
    12421 cttccttcat ttttcgattt cccgttattt tataatctgc tacggttgat aaagaataca
    12481 aaaaaccaag atcgtgatga ttagtgataa tttgacagtc aatcctgtgg ataaaatcta
    12541 tatctgcttt ttcagctgtt tgttgaaata aaggatcacc cgtcttctca aataacaacc
    12601 aattaattcc tgtccaaaat ccttctgtcc aaccaatatt gtctcttaat ggataacgtc
    12661 cttttatagg acactcgctt gggaattgat actgactgtt actgatacgt ttgattaacc
    12721 tctccgtgtg tctgcgacaa cgctcgatct cattattcca ttctttttcc attaattctt
    12781 tataacattc atttatccat ttttctggat gtattatctc ttcaaacatc actttatcaa
    12841 gcatttcccc accagccttt agcataattt tagtcttttt ttattgtttt tattttggat
    12901 agttagcccg gttgtcccat caaaatcaat tttgggacga ttgttccata cttctaaact
    12961 ataatcccga tcttgatgag caaccactcc tgttccgagc caatgggttc cttttccaaa
    13021 tgtaccctct aatccaggaa taaaagtgtt ttcccatgtc atcaagtttg tattgggcaa
    13081 acccataata gctgtggatt ttcgtttaat agtatcgtca cttaaacaga taatacctga
    13141 aaagccagct tcactcaccc tatagttttc cttgtcttct tctatgatta ttccttcttt
    13201 ctcaggagaa cattcaatag caaaccctgt ctcatataga acatattctg tatctaattc
    13261 tactttgtgt acgcgaatct gccagccttc aaaaggaata agccaagttc tgatttttgc
    13321 ctgtaaagta ggttcccatg tagaggcaag tacctctttc ttacatacaa tgtctcgtac
    13381 attatttcga acgatgaact gtccataggt tttgatgctc tcattcttaa aactggtcgt
    13441 gatatatgtg tccgctcgct gaatccctag cattgaatcc attgccattt ctgctttcaa
    13501 ttgattcgct cgcggtatcg aaaaaccaaa ttgactagaa taagcaaatt ttaaatattt
    13561 ttcattggtc aatgtatgga aatttggccc taattgtccc gcattcaaaa aaaatgtatg
    13621 tccattatca tgttgtatga gcaaattggc ttctgaaaga agtttagttc cttcacgaat
    13681 aggcataggc atttctttag ccgtccaata ctcatggtct tcaggtagcg ccaacaataa
    13741 aaaaatctta ttcagccata atggagataa cgtactatta tagggttcag tcaatgtcaa
    13801 ttgagggtag gtataaccaa tattcaggat acctttttca tcaaatattt ctttttgcat
    13861 ccaccatctt atattccgat tgactaaccc cttgatttca cctaatttaa aaggcaaaag
    13921 atcggcccac aacatagctg accaaaagct tgaaactgca aaacgataaa tcatgcttcg
    13981 accaaaaggc acatttgcac catcttccgc aaaaaaatga atatagtctc tagaaaaatc
    14041 aatagctcgg ttcttatata tctggctaat gggcttttct ggttctagta cgctataaat
    14101 caaaccataa taatgaaatg caaaaggatt ataataatct tggcgacctc ttgaactgtc
    14161 tcgataccag ccattttgaa gataacaatt ttctatatta tcaaatgctt ctttcagtgc
    14221 tgattcttta actggataac caaatttttg gataattgtt ccgacaatta tcttaaaaaa
    14281 ttgccaatta ccatctgcac attcatattc aaaaatttgc gagaaccaat gaataatctg
    14341 gcttttttct cctgtatcta attttttcca taataattgt tcatgatgat ataacgctaa
    14401 taacattgcg ggcatttcta ccatacgctg gtctctttca tgtatttctc cccaataatc
    14461 gggatgggaa ggatctgttc cagcttttat tcctgataga attaatttca accatcgttc
    14521 atctaattgg tcaattgcgg gccccgctcc ccataaaacc cgagagaatc cttccattcc
    14581 tgcaatttta tttccatatc ctaccccttg atgtatataa tcaatttgtg cgccacttgc
    14641 tcgataaaac ggctggagtg gttgatttat ctttttaaat aagtcaaagt aatcctgtct
    14701 ggtccttaat agtggtactg gatgtttcat tgtattttct cctcttttta ctaccaaata
    14761 gctaattccg actgttctaa tcgaagtaag ccttcaataa aaaaataatc agaatagatc
    14821 agtggcacat tataagtcag ttctctatgg tatgcttctg ctccgcctcc aatgatccca
    14881 tcttcatcca tattccaatt tgcatagtgc ttttctgtct tttggagtat attgatagca
    14941 gcttcatgat acatttttga tcgtacagag gaaacatatc ctgctaattc cagaagaccg
    15001 catgcagcgc atactccagc actggtatca atattcgaat cttttacagc aaaatcaatg
    15061 agaggaatat aaccagtttt ttcgattttc tgaataaaat ttactgctat cttttctgct
    15121 atagctagat agtacggatt ttttgtgtaa cgataactta acgcgaatcc atataaagcc
    15181 catgcatttc ctctgctcca acaagaattc tcattgtatc cttgcccgcc aagtaaccga
    15241 atatatttcc ctgattctgc atcaaattct gcgatatgcc cggttgatcc atctttcctc
    15301 actagatact tagctgttgt atctgcatgc tgccgagcaa ttttagagaa cctcggatca
    15361 ccagtttccc tcgaagccca atacagtaat ggtaaattca tcatactatc aataatcatc
    15421 caaccaggtt tatggtcatt ccatgctact aaatagttcc ctgaaatatt atagcgtcca
    15481 gcaagtaccg tagctgcatg taaccctagt tgtcgactat gtttgttttg agactgttga
    15541 taatgtgcaa tagccgatgg taagaataaa aaacctacat catgatgaac gtctgaaaaa
    15601 tggtacaggg cagattctaa taattgttct tgttgtattg ctgcctcttt gaacttttct
    15661 cgttttgtct tgtgaaagag ctgccataaa ataccactcc aaaaaccatt ggtccaccag
    15721 tcgattcctc tgtcctttat tacgtctgta tattttccgt tgtaagaaat atatggaatt
    15781 ttattcccca ttctgtctaa ttccgcatct atcttttcag aagctacatt caaaattttt
    15841 cttgaccatt ccttcgtctg ttcattcatg gatatcccgc cattctttta atttttatga
    15901 atgtggagtt gctcctagaa cgaactagat ggttaacatt cgttattttt tttacttatt
    15961 tgcagctcca atgtcattct ccttaaattt agactgcgcc cacacataaa tcaagaccat
    16021 tgggatcact gaagttgcta aaattagcat aattgcgcca taattaattg gctcacctgt
    16081 actagaaacc gtcatatttt tgataattaa gggaaccgta tatttatctt tatctgaaag
    16141 catgactaat ggaaatagat attgattcca acttcccata aagataagta aaccactagt
    16201 aataatcgta ggtgataaaa tcggtacagc gattttcgta aaaatcaccc actcattggc
    16261 accatcgatt ctggcagcct ccatcagtgc tgtaggaaat tgactgctat attgtcgcat
    16321 cataaacact gaagttgtac tggcaatcga agggatgatc acacctgcat aagtattcga
    16381 taatcctaaa cgactcataa tgtaaaataa aggaatcaat gtggtaaaaa aaggcaccat
    16441 cctagatagc ataactgtat tgaacaacag tttattcccc ataaaacgat actttgctaa
    16501 tgcataaccc gccattgtag aactggctgt agctaaaact gtactgatta cagcaataac
    16561 aatcgaattc caaataatca agggataatt atatctcgcc atgatggctt gccagttttt
    16621 taatagcata tccccaaact tgaaattgat tccgccactt aaaatttctt gattactttg
    16681 cgttgaagct acaatcatat ataaatatgg catcaaaaat attaaggcac caaacacaat
    16741 aatgacgtac gtaaaaattc tgctttgtat cttttgttta gtcattttta tcacctccga
    16801 atttaaattg aatagccgat aatataacag atacaaacac catcgtccag gcaatagcag
    16861 aggcttttcc aaaatcaacc atttcaaaac ttgttttata taaattgact cctacgacca
    16921 aagctgaatt ttctggtcca ttggaagtcc caaagaatat atttgggata ttgaaattac
    16981 ccaagccatt gatagtggct aaaactgtgg ccattaaaat gattggcttt agcatgggga
    17041 tcgttaataa aaagaattgc cgaaatttcg atgctccatc tatttcagca ctttcgtata
    17101 tctctttaga aatcccttga agaccagcta aaaataaaat agtgttatat cctacactaa
    17161 gccaaatcat cgtgatgatc attgatattc gtatccaaaa aggatttgtt aaccactgta
    17221 caccgttaat tcctagcgca ttcaaaaatc cattgaataa cccagtcgga ttgaaaaaag
    17281 tcatgaatac ggaagctaca gccaccgttg atgtaatggt tggaatataa tagacagttc
    17341 taaaaaatcc ttttcctttt aactgtgctg aattcaataa aacagcaaag taaatagcta
    17401 atagcatcgt tattggaatt gatcctgcca tgataatcaa aacattcgtg taagatttaa
    17461 taaacaatgg atctcggaag atagctagat aattgtcaaa tccaacaaat tcaaatgagt
    17521 agccaatttt cataaaacta aaagcaaacg agataatgat gggaatcaaa atcgtcacaa
    17581 taaagaaaat aacaaaaggt gtgaccaatc cataagcact ttttttatga gacgtatctg
    17641 ttgctttttt cttgctgttc tttattgtct tgcttggtaa cgaagattgc atcatcgtta
    17701 agtcctccta atatttttta atcaccagtt aattttacgc cgtacttatg agcaaaattt
    17761 tctgcttctt ttagatatga attttcaaaa tcacctgttt gccaatagtt atatgcagcg
    17821 gtagtcaaat aagacgtagc atctgaataa gtggctggta aaacagtatt tgatgtattt
    17881 tcattgattt tattcaatag ttcatagtat ttttgtcctc cttagtagtc aaactttttc
    17941 tcagctgctt ctgtttgata ggccaatgag ttagatgtag ccacgcccag ctccagtgcg
    18001 ttggctaagc attcttcatc tgtcagcata aatgtaagaa attgttgtgc cacttgtgga
    18061 ttttttgcat ctttagggac ataaaaactt gatcctcctg atactgcacc ttttcctgga
    18121 ttttcttttg aaaaaggaat cagtggagcc atgcgccatt tgtcagatgc atcaggaaaa
    18181 ttcaaagaca tattcgtagc ataccagccg ccagcaacaa acattgcaga ttcttgaaat
    18241 gctttctcag catcttgtgc accgtagaaa tgtacaatat ctgagtcgat catttctttg
    18301 attaactttg cagcatgttt cgcttcttta gtattcagac ggatatttcc ttcttcatca
    18361 agcaaagatt gattctgctg ggctagcatg ttgataaaca attcttggtc cccagattct
    18421 ggcaaggcaa ttccaaaact accagttttt tcttttaatg cttttgccac ctggatatat
    18481 tcttcccaag aactgatttc ttcaaaatct acacctattt cagcaaattt ttttggttga
    18541 taataacaaa gtgctggggt aaaatcacta gcccaaggaa taatcgtttc attactcgtt
    18601 tgttgacgca aaatattcaa tttttgatca taaaactgat cgccatattt gtcataaaat
    18661 ccatattcat cagcactata aaaagcatct tgaaattttt ccagcacacc tgctgctgaa
    18721 gtgtctgcca tggagataat atccggcata tcttcgtgtg ttaccaattt tggggtgatc
    18781 atttgtactg ttgctgttcc cgaagctaca ttcacaattt ttaagtcaat atttgctcca
    18841 gttttgcggt tatagatttt tgcagcattt tcaacatagt ttgtcagttt gccgtttgcg
    18901 acatatactt ctaatgtagt ggcttttttt atcattggat ctgcgatgtt tgctgttact
    18961 tcttcgttag tttttccaca tgcacttaag ctgaataata gggtaatgtt tagcaaagct
    19021 atcaccaatt tgtatctttt tttcatgtct atcccttctt tcttttcaag ttcttatata
    19081 aaacgctttc acttatgtat attaacggaa ttagtaacac aaatatttat ataaaaaaat
    19141 attttacttg tcgttttggc
//
LOCUS       DANKHL010000035        19038 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000035 DANKHL010000000
VERSION     DANKHL010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.037"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            81..659
                     /locus_tag="PW101_000868"
     CDS             81..659
                     /locus_tag="PW101_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2513289.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            659..1588
                     /locus_tag="PW101_000869"
     CDS             659..1588
                     /locus_tag="PW101_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2513290.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            1585..3321
                     /locus_tag="PW101_000870"
     CDS             1585..3321
                     /locus_tag="PW101_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL2513291.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(3384..4220)
                     /gene="pheA"
                     /locus_tag="PW101_000871"
     CDS             complement(3384..4220)
                     /gene="pheA"
                     /locus_tag="PW101_000871"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDL2513292.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(4217..4726)
                     /locus_tag="PW101_000872"
     CDS             complement(4217..4726)
                     /locus_tag="PW101_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDL2513293.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(4719..6014)
                     /gene="aroA"
                     /locus_tag="PW101_000873"
     CDS             complement(4719..6014)
                     /gene="aroA"
                     /locus_tag="PW101_000873"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL2513294.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(6027..7118)
                     /locus_tag="PW101_000874"
     CDS             complement(6027..7118)
                     /locus_tag="PW101_000874"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDL2513295.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(7135..8301)
                     /gene="aroC"
                     /locus_tag="PW101_000875"
     CDS             complement(7135..8301)
                     /gene="aroC"
                     /locus_tag="PW101_000875"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDL2513296.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(8303..9367)
                     /gene="aroB"
                     /locus_tag="PW101_000876"
     CDS             complement(8303..9367)
                     /gene="aroB"
                     /locus_tag="PW101_000876"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDL2513297.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(9400..10422)
                     /gene="aroF"
                     /locus_tag="PW101_000877"
     CDS             complement(9400..10422)
                     /gene="aroF"
                     /locus_tag="PW101_000877"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDL2513298.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(11081..12055)
                     /locus_tag="PW101_000878"
     CDS             complement(11081..12055)
                     /locus_tag="PW101_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HDL2513299.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(12036..13919)
                     /locus_tag="PW101_000879"
     CDS             complement(12036..13919)
                     /locus_tag="PW101_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HDL2513300.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(13937..16162)
                     /locus_tag="PW101_000880"
     CDS             complement(13937..16162)
                     /locus_tag="PW101_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL2513301.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            16277..17311
                     /locus_tag="PW101_000881"
     CDS             16277..17311
                     /locus_tag="PW101_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2513302.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(17378..18391)
                     /locus_tag="PW101_000882"
     CDS             complement(17378..18391)
                     /locus_tag="PW101_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2513303.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(18469..18657)
                     /locus_tag="PW101_000883"
     CDS             complement(18469..18657)
                     /locus_tag="PW101_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL2513304.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         5344 a         4113 c         3480 g         6101 t
ORIGIN      
        1 taaaataaaa atatccttgc attttctcct agaaataagt atttttaaat tacaagacta
       61 ttcttaaggc gggtaaaaaa atgccaatac gcgtaattct tatcgatgat gaacagacga
      121 ttttagatgg aatgaaacag ttgatcaatt ggtctgagca cgggtttcaa ttaataggga
      181 ctttttccag tccgctgaaa gcgcttgatt tttctttggt acatgcaatc gatatcgtgg
      241 tcacagatgt aatgatgcct gaattaaatg gaatcgaatt atcgcggcta ttaaaacagc
      301 aacaacctga aacacagatc cttatcctct ctagctatga tgaatttgat atggttaaag
      361 attcttttaa agaaggcgtc gctgattatc ttttaaagcc tcgattgagt cctgattttt
      421 ttctaaattc cttaactgcc gtttcacaaa aaatcaaaaa aaagcccaac aaaagcccaa
      481 tcaccaatcg ttatgagcag atctgcgaga atctttcaca gcagattgct ggacaaactc
      541 gtccattgct tgaccctctt ctttttgaac acaccttgtt tttctgttat ataccgaaaa
      601 acggccattc agttcaaaac aaggctctaa acaaaaacag tcgatcaccc attcattgat
      661 ggatagcgac gattcgttta ctatttatcc tgtttctacc ttttcggaag aatccggcta
      721 tgtaatcaat actgattcca aagatacact taggaagttt gttcaaaagt ttcgtcaaga
      781 agtttttcca ggtgattttt ttgttgtttc tgattctttt gcaatccaag atatcaatga
      841 aatattccaa gagatcagaa attgcaccaa aggacaacgt ttctatcata agggaaagaa
      901 ccttgcttat cagcatgaac ttcttccgtt attaaccaac cacgagcagc aagcaagtag
      961 ttatttaaca aaaatcgttc ataaagacta cgctttagct gtcaaagaac ttgagaattt
     1021 cttcacgacg atattggaac agcctattga tccagcttat ttgaaacatg aggcaatcaa
     1081 tcgtttctat gcattactca acgctttggc tgacgagcat gttgggaatg aagaactttc
     1141 tttgctcaag atcgcccttc cttctttatt agccgatgct gagtatttcg aagatttttc
     1201 tattcttgtc aaagaaacac ttgagaaact cacccacttt tctgcaaatg caaaaaaaga
     1261 caatttcgat atcttgactt tgatctacga gttcgtccag gaaaattatg agaaagaaat
     1321 caatttacaa cttttatccg aaaaatatca tttttcatat agctatctct cagccatctt
     1381 tactgaaaaa tttggtatca acttttcaaa atatctaaaa aaagtccgta tcagtaaagc
     1441 caagctcttt ttgacggaaa cacggcttac tttgacggaa atctgtgaga aagtcggcta
     1501 taccgaactg ggttatttct ccagagtttt taaagaagag acaggaatga caccttctca
     1561 ataccgtaaa ggaacttttg taaaatgaga aaaaaaatca aagagcatga actctttgcc
     1621 aaacttctgt tcattttgtt catcggtctt tttctgcaag ccatcgtcat ttctttgttt
     1681 atttatcatc gatcaaggga tgcctatatc cagctgttca atcaatcgaa tgatgtggta
     1741 ctaaaaaaga ttcaatctga atttgaaagt ttgaatgata caatcgagaa tacattagcc
     1801 gcttttgatt ctaatccagc tgtcaaaagc tatttttcca atgatccagc ccaacatatg
     1861 gaacagcttc agcaactaag aacaatccaa aaaatgaatg actctctttc aaaaatccat
     1921 ccaatgatcg attacgatgt attgatcttt ggagaaaatg gaagaacttt tgtcggaaac
     1981 gacatgctca ctgccgtatc tgccgattct ttttttcaat ctgcgattgc tcagagagtg
     2041 aatgaacgtg ctgctgatac acaaatgctt tttgcacacc acggattgac tcttcgcgat
     2101 aagaaagcac ccagcgtctt ctttgtcaaa aaactgaaaa atacattgaa tcacgtttac
     2161 ggatatgctg ttttatcgat ctcatccaag caactagcga atctttttca atctgtagta
     2221 aatcctgaga tatcgaagat ctccttcatg aacgaagaac agacgatcat cgcatcaaat
     2281 gaagaaaata caattgggag ccgatcaatg attttcaaaa aactaacagt aggtgaaaca
     2341 acgatttcaa atggacagca gttgacccgt cttcctctgt accgccagga ttgtacatta
     2401 ctcagtcaag tcgatcttca ttctttatct aaccaaatgg gcgtgatttt tccgattatt
     2461 ttgtataata tcgtgacact tgcttttgct ggctcattgg tatttatgta tttggatcga
     2521 cacacaaaat cgatctaccg cttgattgat tcgctaaaac ggctagagga agatggctta
     2581 aaagctactg ttcctgtaca aggaagccat gaagtccaat tgctagggaa tacgatcaat
     2641 caattgctaa gtaatgctca gcatcattat gaacagacga tccaagacga aaagaagaaa
     2701 cgttcgcttg aaatccaaac tatgcaagca cagatacagc cccattttat ttataatacg
     2761 ctgactagct tgaaattttt gatttggcag aaaaaaacag atgaagcagt tcaaggaatc
     2821 gacagtttca ttactttact aagaagtacg attgggaaaa aagaagaagt gattccagtc
     2881 aaggaagaaa tcaaaagcgt acaaagttac atcgatcttt tgagtatgcg atatggcgaa
     2941 agaatctcgg ctaaaataat gattcctgat gaactgcagt cattatctat gccgaatatg
     3001 atccttcaac caattatcga aaacgcctat cttcatgctt ttcacaataa aacgcaagga
     3061 ttcatcacgg tgtacggcaa aacaaaaaat cagcttcttt tattcgaagt gattgataac
     3121 ggcgatggct tcgatactga aaataccaaa caaaaaaatg atcatttttc aggaatcggc
     3181 atcagccatg tagatgagcg gatacgccta atgtatggcg atgcatatgg cttaacgatc
     3241 caatcagtga ttgggacagg aacgactgta acgatttgtc taccgcttct tcctaaaaat
     3301 caatcagaat atcaaaaata aagaatagcc aagcgctgat ttttttattt taagttaaat
     3361 aaatcaatca gtattcttga ctttcatcta cgcgtctctt ctactgtcag cactggataa
     3421 cagcccaata tttgactttc tcctccgagc atcttgattt cttggagcgc attctctact
     3481 aatgtcattg gacggtccaa caacaaatcg atcacgaaaa aatattctcc caagctagtt
     3541 ttcaatggtc gggattcaat cttacttaag ttgatttccc gccagccaaa agctgccagc
     3601 attttatgaa gcatccctgg ccgattagca ggtagtgtca agatgacact catttttact
     3661 ggagctgact gctggctggt catctttcgg tcaccgacga tccaaaaacg tgtctggttc
     3721 aactcattat cttggatatt ctcagctaag atctccaatc caacatgctg tgctgtttct
     3781 aatgaagcaa tcgctgctgc gtcttctttc ggatgttctg ctacatacat tgctgcagca
     3841 gttgttgatt ctgttgctac taaaggaaca ttaggataat gcgtttctaa aaactgttgt
     3901 gattgtgcta gtgcttgtgg atgtgaaagg atttttgtaa tctttgttgc tggattgcct
     3961 aaaagctgct gcttgatagg aagaacgatt tcacttttaa cttcgacttc tggatgtttg
     4021 gaaagaagat cgattgtgtg atgaacagct ccttccaatg aattctctac aggaacgaca
     4081 gccagatcga cttgtttgcg aaacaaagcg cgcaaacaaa cagggatgga agcatacgca
     4141 gtcaattgtt ctgtaggaaa tagctgacaa gccgcctgat aagtgaatga actttctggc
     4201 cctagatagc tgacgttcat atgcttttga tcctttctat aatggtatcg atgatctttt
     4261 ctggtgtttg attggtcgtg ttgattcgat gtgtcgcact ttcttcatac caagaaagtc
     4321 gttgtgccaa taatgctttt atttcttctc ttgtttttcc aagagctaaa ggtcgaatag
     4381 acgtttcatc ttgttggata cgatcaacta gacagtccgc ttctgcctct aagtaaagaa
     4441 caatcggctg tgtttttaaa aaacgccgat tttctgcact ttggacaatc cctccaccag
     4501 tcgcgataat tttgtcttca tttgacagct ttcttaaaat atctgactcc acttttcgaa
     4561 agcttttttc accgtaatag tgaaaatatt cggcaatcga caattggata tgttcctcaa
     4621 tctttgtatc taaatctaca tactcttttt gtaaacattg agctaaccct ttaccgattg
     4681 ttgtttttcc agcgcccatg aaaccaatca gcaaaatgct agccatgagc ttctcctcct
     4741 gacacaagtt ctgctaaatc gtcaaaaaat gccggataag agattgaaac agcttcagct
     4801 ccttctagtt cgcaaggtcc atcagctatc aaagcagcta cttgcagcat cataccgatt
     4861 cgatgatcgc ctctgctgga cacttttgcg ccatgcagag gtgtcggccc atggatgatc
     4921 agaccgtctg ctgtcgcttt tatctttgct cccattttct gtaattcttc tgccaccgca
     4981 tcaattctgt tggtttcttt tacttttaat tcttctgcgt ctttgatgat cgtgattcct
     5041 tctgcttgag tagccactaa agctagaatg ggcaattcat caatcagacg cggaatgatt
     5101 tctccaccaa tcgttgcttt cttcaaatga ctcgtcttga cacttaaatc agccgattgg
     5161 ttctgttcat tgtgattcgt ctgggtaatc gctgcgccca tctcctccaa cacatctaag
     5221 ataccagttc ttgttggatt gaccccgaca ttttttaaca acagttggct ttccggaaca
     5281 agaagtcctg ctgctagaaa gaaagcagct gatgaaatat cacctggaac agttatctgt
     5341 tgaccagtta atttttgcgg tccggtcact atgatcgttt tatcttccac tgtgatcctt
     5401 ccgccaaatt gacgaatcat ttcttccgta tggttacggg aacgttcttt ttcaacgatt
     5461 acggatgtgc cttctgcttg taatgccgca aacagcaaag cagatttgac ttgcgcactg
     5521 gctacaggca tattgtatcg gataggtgat aaagagcggg ttcctgtcac agtgattggt
     5581 agatactggt cattttcttc tccttgcaag tcagcgccca tctcgcgtaa aggttccatc
     5641 actcgcccca ttggacgttt agacaacgaa gcatccccga acagagtagt cgtaaaaggc
     5701 tgtccagcca aaatacccat caataaacgt atggtcgtac cggagtttcc catgtcaatt
     5761 gattcggttg gttggacaaa tgagtggcta ccgcgaccat gaatcttgat cacctcttcc
     5821 tcttcttcga tctcgacacc taattgttgg aaggcatgta aagtactcag acaatcttct
     5881 gcgcgcaaaa aattttgtat agtcgtcgtt ccttcagcaa ttgctccaaa catgatgctg
     5941 cgatgggaaa ttgatttatc tgctggtatc ctaacagtcc ctcttaatcc atgtatttgt
     6001 tgtaataatt gcatttatcc agcctcctat ttgatccagc aatgataact ggtattagac
     6061 ataatgattc tttttgcttg taacaaatcc tgttggtttt taaaactgat ttgcagtatt
     6121 cctaaaatat cttccctggt ctcttggatc ttcaaattaa tcaaagaaac tcccgctttt
     6181 ccgatcagtc cagttacttc tgcaataacg ccaggttcat ctggcacatc gataaataaa
     6241 tcaaaaaagg caggaatcgc gccttgctta tggacaggca actggtctcg actttctttt
     6301 gcccgattga aaaaagaaaa gatttccgat tggttctcag tcatgatcca ttctgctatt
     6361 tgtgtcattt cttgttgcca atctgccaat cgttccaata atatctgctt attgctcaac
     6421 aaaatatctg tccacatgac aggatctgag gaagcgatcc tagtgatatc tcgaaagcct
     6481 cctgctgcca gttgtttggc tcgcggatac tcttgattga attgttctgc ctgattgact
     6541 aagccagctg ccagaatatg tggaaaatga cttagcattc ctgttatacg gtcatgttct
     6601 cttgctgtaa gagttacata ttttgctttg gttccgcaaa atagttcttg cagtttttca
     6661 acatcttttt tcgtcttttc caaaggtgta aagatgtaat aggcattttc aaatagttta
     6721 gcatctgctg cctgtatacc tgatttatga gaaccagcca ttggatgtcc accgacaaac
     6781 cgaagatttt tctgctcagc ggatgtcacg acttgctgct tcgtactgct gacatccgta
     6841 atgatgacgt gatcagacaa gttgattctt tcaaggacat caagataagc taaggttgta
     6901 cggacgggaa cggctaataa gataacgtct gcttgttctg cacctgtttc aaaatcactt
     6961 gcaacgtaat caacgatctg ctgttctact gcttgctcac gggtttttga ctgactatcc
     7021 caaccaacta ctatcagttc tggattggtc tgacgaatac aaagtgccat agaagcacca
     7081 atcagtccaa gccccacgat aaaaacatgt tgtgccatct tatttgcccc ctttttagta
     7141 atgtttcaag tagtcacggt actccttcac tgcagctttc aactcaacaa gcgaatcact
     7201 tgggaatttt gacaatagct ctgtcgctac cacagtggct acaacatttt ccgcaacgac
     7261 gctagctgca ggaactgctg tactatccga acgttctaca cttgctttgt aaggttcttt
     7321 tgtatcgata tcaacacttt gaagtggctt gtacaatgta ggaatgggtt tcatgacccc
     7381 gcgaatgaca agtggttcgc cattggtcat tccgccttcg aagccgccta gattattggt
     7441 acgtcggaaa tagcctcttt cttcttccca taggatctca tccatcactt ctgagcctgg
     7501 taatgaacca gaagtgaatc cgaccccaaa ttctacgcct ttaaaggcat tgatactaac
     7561 cacagcttgt gcgattttgg catctagctt cgtgtcccat tgtacgtagc ttcctaatcc
     7621 agctggaacg cctccaacga gcacttctac aattcctccg atcgtatctc catttttctt
     7681 tgtctgatcg atcaaatctt ttattctttg ctcaactgct ggatcaacaa ctcgtacttc
     7741 tgacgcttca gaaatttctt tgatttcttg aatcgagata tttccaggta tttctgcttg
     7801 gattccacca agacaaatca cgtgtcctgc tacgatgatg tcaagttctt ccagaatttt
     7861 cttggcaatt gcgccgattg ctacacgcat ggtcgtctct cttgcagagg accgttcaag
     7921 cacattccga agatctcgat gatggtattt cataccgcct accaaatccg cgtgtcctgg
     7981 acgcggacgt gcaactcgcc gcagtgattg gtctttttca ggcacttctg ctacagacat
     8041 gacagttgtc caatttttcc aatctttatt ttctaccact aaagtgacag gtgaaccaat
     8101 tgttttgccg tgacggatgc cagacgtgat ccgaacacga tctttttcga tcttcatcct
     8161 tcctcctctg ccgtaaccag cttgtctgcg agcgagttcg tgattgattt cttcttctga
     8221 cagaggtaat cctgctggta gtccttcaat aatggctgtt aattctggtc catgggattc
     8281 tcccgcagtc atataacgca tcttacagtc ctcctaagta atctttgaat gtctctgtgg
     8341 gaacaggaac gatttttgct tgcccaatct tttctagcaa gatgatattc aattggcttc
     8401 ctcttgcttt cttatcatgg gttatcgcgt ggaataattc atctggatga cttagatcgt
     8461 acgtaatcgg cagatggaat ttttgaagta tcctttctaa agcagctgtc gatccagttg
     8521 gagacaagcc gattttttct gcatgagaag tgatggctac cattccaata gctactcctt
     8581 ctccatgact gactacccca tatcctgctg tattttcaat tgcatggcca attgtatgac
     8641 caaaatttaa aataagtcga tttccttggt caaagacatc ttcttccact acttttcgtt
     8701 tgatttccaa cgtagcagta atgattggaa cagcatgttc cagaagatct ttttcgtctt
     8761 tcagctcgtt cagctgtttc caaagcgttg catctgctat agctgctgat ttgatgattt
     8821 cagcgattcc ttctcgtaaa cgacgtaccg gcaaagtttt tagaacctca ggatcaatca
     8881 atacaccagc tggctggcta aatgttccga caaggttttt cgctttgttt gtattgactg
     8941 ctgtcttccc gccaatacta gaatctactt gtgcaagtag cgtagtcgga atttgaacga
     9001 aatggatccc acgcatgaac gtcgaggcca cgaatgctgc tagatcaccc accattcctc
     9061 caccaagtgc aagtatccca tcgctgcggg taaaatcatt atctgctaaa aaatcgtata
     9121 actcagctgc ttgttctaaa gatttactgg tctcacccgc cggtacgatt gaaaggactg
     9181 gttcaaaatt agcttgtctc aattggaaca tgattttttc tgcatatagt ggagcaacgt
     9241 ttgtatcagt gatcacagct actttttgag gtctccaaat cgtttgaagc catgatccag
     9301 cctgttctag tgcgcctttt tcaatatgaa tatcgtattt ttggttatca acatgtacag
     9361 taagcattcg tttcacttct ttcttttaaa aagctggaat taatcgagtg tctgaatctt
     9421 ttccattgct tctttcatga tcgcaacttc tttcatcatg cgtgagtaag tcttttcatt
     9481 caacgattgg ggaccgtctg accaagcatt tgcaggatca ggatggatct ctacgatcat
     9541 gccatctgct ccgcttgcca cacccgcacg agccattggt ggtacaagtt cccaaattcc
     9601 tgtaccatga cttggatcaa caatcacagg gaaatgagtt aattttttca tcaatgggac
     9661 agcacttaaa tcaaacgtat tacgtgtcgc tgtttcgtaa gtccgaatcc cacgttcgat
     9721 aaaaatgaca ggagatttgt cttcgactgc aatgtattct gcagcattaa gccattcatt
     9781 gatcgtaccg gaaattccac gtttcaatcc aacaggttta cctgttttac cgacagcaga
     9841 tagcagttta aaattttgca tattacgtgc gccaatctgt aggatatcgc tgtactcagc
     9901 caccatatcg atatgccctt catccatcac ttcagtaatc actttcattc caaattcatc
     9961 agccgcttga cggatgtatt tcaatccttc ttcttctaaa ccttggaaag catatggaga
    10021 agtacgtggt ttgaaggcac cacctcgcag aatttttgcg cccccggctt tggccattcg
    10081 cgcacattct ctgatttggt ctaatccttc gatagaacaa ggaccagcca tcgtcacgaa
    10141 actgccgtca ccaattttta ctccgtctac atccacgact gtattgccag gatggaattc
    10201 tcgagaagtc aatttgtatg tgtttaaaat acgcacagca ttttctactc cttcatagcg
    10261 tgtaaatgct ccttcttgta aacttcgcgt atcacctttt agtccaatca ctactcgttc
    10321 tgttccttga tcgattgaga ctttgaatcc ttctttttcg atatgatcga cgactttttt
    10381 tatttgttct tccgttgctt cttttttcat aattactatc attgtttttc ccgctttctt
    10441 ttgtttactt atctatcttg gctgattttc tctacttttt caaggtggtt ttacattaat
    10501 tcctactaaa tgaccgtgtc ctgttgttct cattttcgct ccttctttcc tacttatgac
    10561 ccagataatc aacaaaaaaa gatgtccctg taggaaattg cctctacagt gaacatcttc
    10621 aacgagcctt aaaaagcgct gcaaaaatat aaaatagcca ctatagatga atttctctac
    10681 gtggctgtaa gtcgtgttta aaaatcttta gccatcatag atacaaacct ggtttgtttg
    10741 tacccacgaa gtcatgaata caaacctatc taaaatagct aaagtaatac gtattcagtt
    10801 ttgtaagttt tctagatttc ataaaaaatc gctccaaact gatgtaagaa ttgtacctag
    10861 tatagacata aaaaaatcga ctgtcaatca tttttatgaa acttttttta attttctgat
    10921 agttaacttt gtttttcaga aaaataacaa ttggttttat tgaagaaaat agtcatgaac
    10981 aaaagtatct gagaactttt ttactctctt gttcatgaca tatagaataa agcagctttg
    11041 tcttgtaaac tccgtttttc aaataataat ctaaagattg ctactgaata ataaagccga
    11101 tttcatggtc ctcttcgctg ctgattcggt aaggtgactc aacatccgct ccccagctat
    11161 tgataccccc gactcctctt acttttgcaa aaatcgtaag aaccgttcgt ctttctggcg
    11221 gtagttcttc tatatgtgtt gcctgttcca gttcttctgc tttgaatggt aaagcggaaa
    11281 aagcaaatgg cgtatcctgc tgttcaaatc gcaacgaaaa atcttcttga tctttatccg
    11341 tattatccaa cgtttgattt cttgttagtt ctacccattt cgtttccata tgcatgccgc
    11401 attcttgggg aacaaggtat ggggaaacgg ggagtcctgc cactttataa ataccttcga
    11461 caccaccagc catccgatcc ggatatgttt cccctgatag gccttcatac tcaaaaaatt
    11521 ttgctttcgt tggcataatg aaccttaaac ccaatgcggg taattccggt aatccttttt
    11581 gtccataata atgcatattg acaagcattt taccctctcc agcgatttgg taggaaattg
    11641 tcacttgggt ttttggtact gtgttgattt ccagtgtaaa aatgatttca accgtttcag
    11701 cgtattcatt acttgaatat tgattattta ctggagcaat cggcaaatcg atctttttat
    11761 tgtccaccag tacttctatt tctttgactt ttggatacaa tccggctcct aaccacatac
    11821 cggatttaaa tccaaatccg ctacctctgt cattatcggt cgttgctcgc caaaaaactg
    11881 gaagtgtttc ccggtatagc cattcttttc cgtttttatt gagcgattcc agtccgcctc
    11941 ttgtataaga aaaaatataa tgcgtattcc ccgatttgac acctaatgta cagtcgccga
    12001 agacgattgc aactttttcc gtatccatat gacttttatt tactgtattt ttcataataa
    12061 tatcttacct cctgcatggc gggtttttct tgtccatctg caaaaacgat cccattccct
    12121 gagaattcat agtcacttgg tttatcgtca aagtcaccgc catagcgcat cacttctctg
    12181 cctgtgattg gatcagtcac ttggatcgcc tgatctttaa aatcccatat atagcctcct
    12241 tggtacattt cataacgatc aagcaacgtc atgtaagaat ccattccacc taacgaattc
    12301 cccatgtcat gcatatattc acataagatg aacggttttt ccgctttatt ttcaaggtag
    12361 tcaatgatct cttctggtgg cgcatacatg cagcttttta catctgaaat cgaatcttcg
    12421 aatgctggat ttggaaaatt cccttcataa tggacaagtc gatcggggtc tacttttttg
    12481 aagtaatcgt tcatttgtcg aatgttttct tccgcataag attcatttcc aagagaccaa
    12541 aaaagaatac ttggatggtt ttttaatgtt tcgaaattcg tttttgctcg atcaagaaca
    12601 gccgcctgcc actgaggtac tgcacctggt acgttgtaag aaggctcaaa tgcgcccatt
    12661 ttttgccatg agccatggga ttcgaggttc gtttccgcca tcacatggat tcctgcttgg
    12721 tcgcataaat aataccaagt gatctggttg gggtaatgag atgtccgtac gccgttgatc
    12781 tttgcatttt ttatgatagc caaatctttt tccatatctt ctatagtgat ggcgcgtcct
    12841 tttttcggat gccactcatg tcgatttacc ccgttgataa tcatccgttt tccattgaag
    12901 tagatgattt tatctttgat ttcgatttta cgaaaaccga aggaatagtc aatatactcc
    12961 gttactcttt tttcttcgtc tatcgtctcc aattcaagct tatataaatt gggctgaaaa
    13021 tgcccccaca gtgccacatt ttcaaatcca tcgatgcgaa tcgtcatttg ctcttttaac
    13081 ggatattgct gttgatagat ttccttccca tcaggatcta gtatcgctac attcagctga
    13141 ctgtttttgg ctacttgctc aaaagacaaa tctacttcaa gtttgccatt ttgacatttg
    13201 tcatctaagc gaggtttgat ccaaagatct tctaaatggt tttgtcctcg agcaactaag
    13261 aagacatctc tgaagatccc aaaaaatcgg aaaaagtcct gatcttccaa gaatgcagct
    13321 gtacttcttt tgtaaacctc cacagctaga atattttctt cttccaaata aggtgttaaa
    13381 tcaaattcag acggagtgaa cgaatcttcg gcatacccga taaaatgacc attcaaccaa
    13441 ataaacattg cttgttctac accagcaaaa tgaatcgaga tttgttttcc ctttagacct
    13501 tcttctaatc gaaaagtcgt caaatacgaa ccaaccggat tgtctttagc agctgaaaag
    13561 atcccttccg cctctttctc agggcttgtc gtgaaagggg gacggcgaaa tacatgtccc
    13621 tcccacggat acatcgtatt gatgtaatgg atcttgtcat aatttgctaa ttcaatatga
    13681 ctaggaacag tgatttcatc aaaagaacag gcatcaaaat cttttttata aaatgttttt
    13741 ggtcgtaccg aaggatttgg cgaataacaa aaacgccatg atccatttaa agactgttta
    13801 agggaggatg tggtttctac ttcttccctt gttgcataac aaacatgatc gctatgtgca
    13861 tttgattggt tcactctaaa aacttgtgga tcatctaacc attttatatt tgctttcata
    13921 cttttttcct tctctctcat aactttttga aatgaagcag ttctgtttgg aaatcacctt
    13981 gaaaatcggg gaagtaaagt ccaactgtca tcaattcggc ccccgaaaat atccgcttcg
    14041 aggtctgttc ctgataaagt gcctcatcat ctaaccccat cagatatact tcatggaacg
    14101 ctggttgagc tgaagctagt atcctcccta aaaacacaat agcttcatct tgtttaggag
    14161 acacaaacat ccatgcggca atattttctt caaaaggact cgccaaacga taataatggc
    14221 catactggat caccggacga atcgtttgat aggatataat ttggtctttt acttgctttt
    14281 tttcttcagg agacagcttt gtcaaatcca gttcataacc aaaaacagcg ctcatagctg
    14341 tttctcctct tgttttcaac gaagtagatc tgtcggtctg atgatttggt acagctgata
    14401 catgagctgt gatggacgaa atcggataag caaggcttgt tccatattgg attttcatac
    14461 gctccactgc atccgtgtta tcacttgtcc atgactgcgg catataataa ataaacccgg
    14521 catcaaaccg tccacctcca ccagaacagc cttcccataa gatttccgga taatcagttg
    14581 tcaaatgttc aagtagttca taaagcccga gcatataacg atgcgctact tctccttgtc
    14641 tctgtgggtc aagggtaatc gaatagacat ctgaaagcga acggttcata tcccatttga
    14701 tgtagtcaag aggaatcgta tctaaaatct ttctcatttg tttctcaatc gtctgccgaa
    14761 catctaacct tgtaaaatcc agtacatgtt gtgatctgga agcagaaggc attctacctg
    14821 gctcctgcat aagaaaatca ggatgtgttc gatacagttc agaatcaacc gagatcattt
    14881 caggttcaaa ccataaacca aatttcaatc ctttgctatg agcataatct gctatttcac
    14941 gtaatccatt tgtcaacttg ccttcatact cgaaccagtc tcctaatgaa gaattgtccg
    15001 catctctttt tccaaaccag ccatcatcta atacgaacat ttcgatgcct agctcctttg
    15061 cttcatcaat gatcgccttg attttttcac tattgaagtc aaagtaagtg gcttcccagt
    15121 tattgaccag aatcggccgt tcagcaaatt gatgacgacc tcttgcgatt ctttcacgta
    15181 gcaactcatg gtggatctga ctcattttgt tcaatccctg acttgagtaa gaaaggatca
    15241 tttcaggtgt ttgaaaagac tggcctgcat tcaattccca cgaaaaacga tggctgttga
    15301 tcccaccgac gactctcaat tgattgatct gatctttttc aagttcgaaa gaatggttgc
    15361 cggaataaac aaattgaagt ccgatcgctt ctccagtatt ttctgtcgta tgatgatgaa
    15421 ctaaagcaat aaagttattc atatggtgac tgctcgttcc tcgacggctt tcaaatactt
    15481 ttgttccaga taaaacagat tgccgtgaaa tctgtctttc attgacatgt gctcctggca
    15541 aagcaatgac ttcatcaaat cgcctagtat gggcaaagtc tagctggaaa ctagccgctt
    15601 tttctagttt gattgtttct ccggtttcat ttcttatctt tacagatcgt gtgatcactg
    15661 ctctatcagt aaatatggta tagaacaaat cgaaataaat cgctgtttct ctatccttaa
    15721 gtgaaatcac caacgtttgt gcctctgact tgtccatcac ataagtttga ggcaagcctt
    15781 cgatctctgg cttcccatct gtaatataat ggctgtcata tctcagatcc agtaagtctg
    15841 atccattctt gcgctgaatg atcgatgcgg gcgttcgaaa gtccatgctg ccatgagaag
    15901 agtattcttg cggtagtgta tctttcgaca gagaccggtc aggatttaag ggaacatttc
    15961 ctgaaaatcc acgatctctt cggggataat ttttattgtc tttgtactgc ttgactggtt
    16021 tgccaaaata aatatgcgcc aatacattgc tttcttctat ctttatcaga taagaaagaa
    16081 atgtatttcg taaatggaag actcggctct tttcatcaaa taagatgaaa tttttatctg
    16141 tttttctatg cgtaatatcc atcatttcac tcccaacaaa acggaaaccg tttacttttt
    16201 tttataattc ctgtatactg tttactaaac aattttgtca ataaaaattt tttatttttt
    16261 tgaggagtgt gtaaaaatgg ttggaattag agatgtcgca cgaaaggcaa atgtctctcc
    16321 tgctacagtt tcccgtgtgt tgaataacga tcagacgatc catgtggctc agactactcg
    16381 gcaacggata gtagaggcag ctgctgcgtt ggattatgac atcagtactc gtaaatatat
    16441 caaaaagaga atgccttcga ttggtgtgat ctctacgatc ggcaaagcga gcgaagaaca
    16501 ggatatctat tacaaacatt tgcgagtagg gatggaagaa gaagctcgac gtctgcattt
    16561 agggatgaat cgaatctata atctatctga taatcctaaa caatgggaag atctagatca
    16621 gttaggtgcg gtgatcgttg ttgggactgt tacgaaagaa tcgattaccg ccttgttaaa
    16681 acaaaacatg caaatagtcg tcgtggataa ccctgacatc cagcaagagg tcgatatggt
    16741 gtatgcagat cttgaacgga tgactaaaac cgtactggaa ttgttcttag aaaatggtca
    16801 taaaagaata gcctatatcg ggggatataa agtcgatatg gatgaattcg gtgggaagca
    16861 gatttctata aatgaaaaga ggatgcgtgc ctatcagcat tttatgatcg atcatcactt
    16921 agaaaaatat atgtcttatc atctaggtgg ctggacagaa aaacaaggtg aggtacttgc
    16981 gactaatcta ttagaaaatg aatcccctct tcctactgcc attcttgtcg ggagcgatcc
    17041 aatggcaatt ggtgtctatc gggcattgaa acaaaaaaat atccaaattg gagaagagat
    17101 tgttgtagca agctttgata atatcgagac aatcgcaaac ctctcccctg gcttgaccag
    17161 tgttcaaatc aatgcgaaat caattgggca agcagctgtt cgaatggcat tggagcgaat
    17221 tgacggtata cgggacgaac caatcatttt gacttatcct gcacgattga ttgttaggga
    17281 aagttttatc ccaagaacaa aaaggtattg aaagagaggt tgtgaaaagc cgcttcgttt
    17341 cttcatactc aaaacgaaac ggctttttca caacctctta cttatactta ttcacaaaat
    17401 ccaatgtctg ctggtaacaa ttttgcgatt cttttagcgt gtaatcaaat tggtattcat
    17461 gggtaatctc tttttttgta tctgtataaa acaaggattg cacgggtacc tttaattctg
    17521 tcaaccgatt ttctaatgct tgaccttgct cctggaatga gtatgcattt ccgtcagtta
    17581 tatacgttgg cggaaattcc ttagaaacat ggtcagccac actagcttct ttcaattgct
    17641 cactcgtttt ccaatctttt gttccaatca acgaccacgc aacggttttg acgaaaaact
    17701 tcataaatcg atcatccgaa tgctgctgtg ctgttagctt caaatcaaca ggtccgcaat
    17761 aagaaatggt tcctttcaga ctatcttttg gaagtagctg tttgattttc atttgtttag
    17821 catacgcatc attcgtttgt atagctgcat attgcaatgc gatttgtgaa cctgcactat
    17881 ctccaccaaa aaataattgt tccatgtcta gcaatttgtc gtctttttct tcagctaata
    17941 aatctttgat cagttcattc acttgaagaa cttgattagg gtattctgaa tcaggcgcta
    18001 attcataatt catcgcaacc actgcgatgt tggcgtctga aactaatttt gtcgcaaatt
    18061 ctttgacacc cgatttatct ccgccgacga atcctccacc atgtacccaa aatagaacag
    18121 gaacaggccc cttgctgttt tttggatagt agatgtcata agtattctct ttatacttgg
    18181 actcatacgt caaatctgtt tttttcgcca cgtttttttc tgcttgatcg aacgtttttt
    18241 tatcagtgat ttgaacagta tttgaaaaca tatggttgat caagaatgct ccaggtctgg
    18301 gggaaagctg gaaggcaaga aagattgcaa acgcgatccc aagaatgatt ccgaagaacc
    18361 atccgataaa tttaagaatt ttctttttca taaggctccc tcagtagtta gtattaagca
    18421 ttctgattat accatagtgt tatttcctat caagctctcc tagatgattt aatgattttc
    18481 cccaaacttc ttctgcaatg cttttgacaa aagaaatttt ttccattgtt cttcaaacaa
    18541 aacattttct tcttctgttg aagcaaatcc acattgagga cttaagcaca aacgatccaa
    18601 aggcagatat cgtgtcgctt cgtagatccg gtcaatcact tgctggtgct cttccatttc
    18661 gccatttttc gttacagtca aaatctaaga caactaattt atcttcagag actttctcta
    18721 aaagaacaaa actcccagca cgttcactat cgtattctaa ataataggca cggacatttt
    18781 cttgagtaaa ataatgggtc tgctacgtcg tcatcccccc tgatgccgcc aaaatcgaat
    18841 aataatttcc gcgtcaaaca tgtatattta gtgttgaatc atttgagaat atgaaaataa
    18901 aaatcttttt gatcaatcag ttaatcaaat tgaaataacg aaaaatctga gacagaatga
    18961 cgttcattct gacccagatt tgattttgca caactcaaca gtccttattg gcaaatcgcc
    19021 aaaataatgt agactaac
//
LOCUS       DANKHL010000036        18933 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000036 DANKHL010000000
VERSION     DANKHL010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.038"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(43..1182)
                     /locus_tag="PW101_000884"
     CDS             complement(43..1182)
                     /locus_tag="PW101_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDL2513305.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1581..2879)
                     /gene="eno"
                     /locus_tag="PW101_000885"
     CDS             complement(1581..2879)
                     /gene="eno"
                     /locus_tag="PW101_000885"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDL2513306.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2995..3750)
                     /locus_tag="PW101_000886"
     CDS             complement(2995..3750)
                     /locus_tag="PW101_000886"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDL2513307.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3854..5044)
                     /locus_tag="PW101_000887"
     CDS             complement(3854..5044)
                     /locus_tag="PW101_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDL2513308.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5154..6155)
                     /gene="gap"
                     /locus_tag="PW101_000888"
     CDS             complement(5154..6155)
                     /gene="gap"
                     /locus_tag="PW101_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL2513309.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6196..7287)
                     /locus_tag="PW101_000889"
     CDS             complement(6196..7287)
                     /locus_tag="PW101_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HDL2513310.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7436..8770)
                     /gene="rpoN"
                     /locus_tag="PW101_000890"
     CDS             complement(7436..8770)
                     /gene="rpoN"
                     /locus_tag="PW101_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDL2513311.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8830..8901
                     /locus_tag="PW101_000891"
     tRNA            8830..8901
                     /locus_tag="PW101_000891"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8863..8865,aa:Arg,seq:ccg)
     gene            complement(9093..9977)
                     /locus_tag="PW101_000892"
     CDS             complement(9093..9977)
                     /locus_tag="PW101_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL2513312.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(10064..10963)
                     /locus_tag="PW101_000893"
     CDS             complement(10064..10963)
                     /locus_tag="PW101_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDL2513313.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(10980..11486)
                     /locus_tag="PW101_000894"
     CDS             complement(10980..11486)
                     /locus_tag="PW101_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDL2513314.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(11675..12295)
                     /locus_tag="PW101_000895"
     CDS             complement(11675..12295)
                     /locus_tag="PW101_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDL2513315.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(12371..12808)
                     /locus_tag="PW101_000896"
     CDS             complement(12371..12808)
                     /locus_tag="PW101_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDL2513316.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            12971..13801
                     /locus_tag="PW101_000897"
     CDS             12971..13801
                     /locus_tag="PW101_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDL2513317.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            13785..15152
                     /locus_tag="PW101_000898"
     CDS             13785..15152
                     /locus_tag="PW101_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2513318.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            15171..15980
                     /gene="yidA"
                     /locus_tag="PW101_000899"
     CDS             15171..15980
                     /gene="yidA"
                     /locus_tag="PW101_000899"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDL2513319.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(16023..16466)
                     /locus_tag="PW101_000900"
     CDS             complement(16023..16466)
                     /locus_tag="PW101_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDL2513320.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            16600..16971
                     /locus_tag="PW101_000901"
     CDS             16600..16971
                     /locus_tag="PW101_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2513321.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(17140..17922)
                     /locus_tag="PW101_000902"
     CDS             complement(17140..17922)
                     /locus_tag="PW101_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDL2513322.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(17919..18890)
                     /locus_tag="PW101_000903"
     CDS             complement(17919..18890)
                     /locus_tag="PW101_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDL2513323.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
BASE COUNT         5223 a         3944 c         3404 g         6362 t
ORIGIN      
        1 taatgctaat ctggctctgt tttttcattt attaaaaaaa tcttacgcta agtaggataa
       61 aataattcct tgatattttt cgatcatatc caaataatta tcgatttcaa catactcatc
      121 tacttgatga ggcaaagtaa cgacaccagg cccaaagacg acaaagtcga aggaatggtt
      181 agctttcgta aattcggctg catccgttgt tgctgccgct ccaactaaag gtaatggctg
      241 gctgaattgt tgctgaatgc aatgaatcaa aggagaatct ggatcagctt tgacaggtat
      301 tttgttgtaa tcgatcatca attccaaatg atagtctgtc tcttgattca attcattgac
      361 gattgattgc agcaatgcaa tgattttatc atttgggtat tctggaatcg atcgaatgtt
      421 cccttggagc tgcgcatggc ttgggatgct gttgacttga ttcccgccac tgatcaatgt
      481 aacattatgg attgtttttc ctaggacagg attctcgatt gtttcagcga ggtgattcat
      541 ttcagcattt gctttcgtaa tgaattcatt taggtgattg attgcattat aaccttggtc
      601 tggcatagag ctatgcgctt cttttccgtg cgaggtgact gtatagttga ttgatcccat
      661 gtgcgtatac atcaaactgt aattggttgg ctctccgatg atcaaggcat ctaagtcatc
      721 tacatagcct gctttcgtga gctgttcccc acctagttca cccacttctt ctccgacagt
      781 ggctaataat ttgactgttc cgttaaatgg ttttcctgac tctttcagtt cgatcatggc
      841 aatcaccatt gctgccaagc cactcttcat atctgtagca ccacgtccgt acaaacggtt
      901 cccatggatt tctgctgcaa agggtgcata ggtccaagac gattcatcac cagccgccac
      961 gacatccata tgcccagata ggcctaacac ttttcctgat tggccctttt ggtaacgagc
     1021 aatcaaatta tctctaccat ctctgtaaga aacaatctct cctgttatgt catgctttgc
     1081 caaaagctta ttcaaatagg ctgctacttc accttcgttt ccattcactg atttaattcg
     1141 gatgatctct tgtaaaatcg cgattttttc ttcttttttc atctaatcct ctcccttgcc
     1201 atttcttttt attgtatttg agtgtgctcg aattttcaaa taggagcaac caaagtaaaa
     1261 atgaagaaag agagccactt tatgaaaaat atgctgctac ttccctccta ttttatttat
     1321 acgagaggac ttgctcatag attcctattc tactgttcta tccttaaaag ctcttatatg
     1381 atgctagtta ttgttagctt tcctttttca aggatgatct tttcttgtac cattctttgt
     1441 aaacatcata tacagaagct agttgcaaaa aaactctcta cctgctgata tttcagcaag
     1501 tagagagttc gattacttaa ctaagtgatc atactcgact atctttcaat agtcgatcat
     1561 gtatcttcat tagaaaagaa ttatttgttt tttaagttgt agaaagattt caaacctttg
     1621 tattctgcaa cttcaccaag ttggtcttcg atacgtagta attggttgta tttagcaata
     1681 cggtcagtac gtgatagaga accagttttg atttggccag cgtttgttgc aacagcgata
     1741 tcagagattg ttgaatcttc tgtttcacca gaacggtgag atacaactgc tgtgtagcca
     1801 gcttctttag ccatttcgat agcttcgaaa gtttctgtta atgtaccgat ttggttcact
     1861 ttgataagga ttgagttagc aattcctttt tcgatacctt cagataattt ttgagtgttt
     1921 gttacgaaca agtcgtcacc aactaattga actttgtcgc ctaatacgtc agttaatttc
     1981 ttgaatccgt cccagtcgtt ttcgtctaat ccgtcttcga ttgagatgat tggatattta
     2041 gaaactaatt cttcgtagaa tttgatcatt tcgtcagttg ttttttcgcc ttcgcctgaa
     2101 tcagctagta cgtaaacacc tttttctttg tcgtagaatt ctgaagaagc agcatccata
     2161 gcaagaacaa cgtctttacc aggtacatag ccagcttttt cgattgcttc gatgattact
     2221 tcaaaacctt cttcgtttga accaaggttt ggagcaaatc caccttcgtc accaacagaa
     2281 gttgctaatc cgcgagcttt caagattgaa gctaatgcgt ggaatacttc agcacccata
     2341 cgtagagctt ctttgaatgt tggagcgcca acaggcatga tcatgaattc ttggaagtcg
     2401 attgagttat cagcatgtga tccgccgttg atgatgttca tcattggtgt tggcaatact
     2461 tttgtgttga atccgcctag gtagtggtat aaaggtactt ctaggtagtc agcagctgca
     2521 cgtgcaacag cgatagaaac acctaaaata gcgttcgcac ctaatttacc tttgttagga
     2581 gttccgtcta aagcgatcat agctttgtcg atagccattt ggtcacgaac atcgtagcca
     2641 atgattgctt cagcaatgat gttgtttacg ttgtcgacag ctttagttac acctttaccg
     2701 ccataacgag ctttgtctcc gtcacgtaat tcaactgctt cgtattcacc agttgaagca
     2761 cctgaaggaa ccattccgcg gccaaaagct ccgctttctg tgtatacttc tacttcgatt
     2821 gttgggttac cacgtgagtc taagatctcg cgagcgtaaa catcagtaat aattgacatg
     2881 ttttgtctct cctttgagtt tgttttttcc taagggattt ccctcatata cgattttagt
     2941 taattttgct cattagtgca aacatttctt gcatttttcg caaattttca acaattattt
     3001 tacagcgtct aacaatgaca agaatgaatc tgcttgcaag cttgcgccac cgactaaagc
     3061 accgtcaaca ttttcttttg ccatgtattc agcaatattt tctggtttta cagaaccgcc
     3121 gtattggata cgaactgctt ctgatacttc ttttccataa agtttttcaa ctgtttgacg
     3181 tacaactcca caaatttcgt cagcgatttg tgcgtcagct gatttaccag taccgattgc
     3241 ccagattggt tcataagcaa taaccatgtt tgacacctgt tcagctgaaa gatcagctaa
     3301 accagctgta atttgtcctt cgatccattc agcagttttg cctgcttcat aggtttctaa
     3361 agattcgcca cagcatagga ttggtgtcat accatttgca aagatagctt tcgctttttt
     3421 attgatgtct tcgtctgttt catggaaata ttcacgacgt tctgaatgtc cgatgatcac
     3481 ataatgaaca cctagatcag caagagctgc tggtgaagtt tcaccagtga acgcacctga
     3541 gttttcccag tagcagtttt gtgctgagat ttttagttct gtaccttcag ctgatgtcat
     3601 taattcttgt aagaataatg caggagatcc gatgactgaa tcaactttgt catttgaagg
     3661 gattttgttt tttactgctt cagcaaattc ttttgcttct gatgcagttt tgttcatttt
     3721 ccagttacct gcgataatag gtttacgcat tgaggaacac actccttaat ttattgaaaa
     3781 atggctaggg agtcatgaaa actcccttca ccttttgttc gattactcgt cagaattgag
     3841 caaacttggt cgtttattta tcgttgattg ctgctagtcc tggtaattct ttaccttcaa
     3901 gcaattccaa gcttgcgccg ccacctgttg agatgtgtgt gaatttgtca gcaaatccta
     3961 attgttctgc tgctgcggct gagtctccgc caccgatgat tgttgttgcg ccttctaagt
     4021 tagcgattgc ttcacaaaca ccgattgtac ctttagcaaa gttactcatt tcaaatacac
     4081 ccataggtcc gttccaaaca actgtttttg caccttgtaa tgtatcagca aataatttta
     4141 ctgatgctgg gccgatgtct aaagccatca aaccatcagg aatgtctcct tcgattactt
     4201 gtgtttcaac atcatttgaa aattctggtg cacagacatt gtcgattggc aagacaagtt
     4261 tgtcgcctgc tttttcgatt aattcttttg ctagttccac tttatcagct tctactagag
     4321 agttaccgat cttgattcct tttgcttcgt agaatgtgta agtcattccg ccaccgatca
     4381 agattttatc tgctttagga atcaagtttt cgattacgcc gattttatca gaaacttttg
     4441 cgccaccaag gattgcaatc attggtcgtt ttggttcttc tactgcttca ccgatgaact
     4501 tgatttcttt ttccatcaag aatccagcaa ctgttgggat accagttgaa gcgataccta
     4561 cgttagaagc atgtgcacgg tgagctgtac cgaaagcatc gttaacaaat acatcgccta
     4621 atgaagccca gtatttacct agttctgtat catttttgct ttctttttta ccgtcaatat
     4681 cttcaaatcg tgtattttcg aatacgacaa cgtcgccgtc attcatgttg cggattgctt
     4741 cttctaattg ttctccgcga gtttcaggta cgaatgttac ttctttacct aataattcgc
     4801 ctagacgttt tgcaactgga gctaaagatt ttccttcttt atcttcttct gtttttacac
     4861 gtccaaggtg agagaaaagg attgcttttc cgccttgttc tagaacgtag ttgattgttg
     4921 gcaatgctgc tttgatacga gtgtcgtcag tgatcacgcc gtctttcaaa ggaacgttga
     4981 agtcaacacg gacaagaact tttttgtctt ttaaatcgat atctttcact gttttcttag
     5041 ccattttatt tcctcctgaa taaacttatt atcaaaaaaa gcggaagaag cgcgattgct
     5101 tccccgcttt taaatgtaca tgtctataat ttacacttgt acgtttgtct atcttataag
     5161 ttagcgaagt attctaaagt acgtactaat tgtgcagtgt atgacatttc gttgtcgtac
     5221 caagcaacag ttttaactaa ttgtttgtcg ccaactgtca ttactttagt ttgtgtagca
     5281 tcaaataatg aaccgtaagt cataccaacg atatctgaag aaacgatttc gtctgtgttg
     5341 tatccgtaag attcgtttga agcttctttc attgctgcat tgatttcgtc aacagttact
     5401 tctttttcaa gaactgttac taattctgtt aatgaacctg ttggtacagg aacacgttga
     5461 gcagctccgt ctaatttacc gttcaattca ggaattacta aaccgatagc tttagcagca
     5521 cctgttgagt taggaacgat gtttgcagca gcagcgcgtg cacggcggaa gtcaccttta
     5581 ggatgaggtc cgtctagagt catttggtca cctgtgtaag cgtggatagt tgtcattaat
     5641 ccttcaacaa caccaaattt gtcgttcaat gttttagcca taggagctaa acagttagta
     5701 gtacatgaag cacctgagat aactgtttct ttaccagtta atgtttcgtg gtttgtgtta
     5761 taaacgattg ttggaacatc gtttccgcca ggagcagaga taacaacgcg tttagcacca
     5821 gctgttaaat gtttttcagc tgctgattta gaagtgaaga aaccagtaca ttctagaacg
     5881 atatctacgc ctagttcgcc ccatggtaat tcttctgggt tgcggttagc taatacttta
     5941 acttcttttc cgtttacgtt gaatgaacct tcatgaactt caactgttcc gttgaaacgt
     6001 ccttgagttg tgtcgtattt taacaagtgt gccaacattt tagcatctgt caagtcgttg
     6061 attgctacta cttcgattcc atctacatct tggatacgac ggaatgctag acgtccaata
     6121 cgtccaaaac cattaatacc tactttaact gtcatttaag atttcctcct tatgaaaatc
     6181 aaaaaaatat ttattttaaa gggttacccc ttttaaaatc tcatttgcag cagcttcatc
     6241 agtgatgagc cacgtttgtt ttggtgcatt tttcatataa gctcgaattg ctttagcttt
     6301 tgattttccg cccgcgattg ccatgatgat cgggactttt tccaaatctt gcaattctat
     6361 gccgattcta gggaccttat aaacgacttc acctttttca ttgaaaaagt agccaaaaga
     6421 ttccgcaaca gcatttgctt gtttcagcat gactaattct ttttcagtca tcttacgacg
     6481 tgctgccatg tgtaatgcac gcccaatact atgaataaca caattcgctt cactaatcaa
     6541 gtttaagact tcttggatag atggctcatt gagtaaagaa ttgtaagttg ccagacttaa
     6601 ttgttccgga acataaagtg cacggtggtt accgtttgct ttagcagcca ttcttgcact
     6661 gacagaattt gcctgtacag ttatcgcttc accaatcccg cctcttgcag ggacaaacag
     6721 gttatgccgt ttcttatttt ccagattaga caattgttcg gctaccacag ccatagtagt
     6781 tcctcccatc acagcgatga tattctcgcc ttctggaagt agtcggtcta gggcttcgct
     6841 cagcgcttcg ccgaatgcat cagccacttt ggtctgctct tcgctgtttc ctgaaacaac
     6901 gatacagcgt tggttaccaa aatattctgc caactgttgt tcggtttgat gcgtacctaa
     6961 aagctgatcc atgaagtttt caaggctttg ataaacttct tcgcccttag ttgtcagcgt
     7021 cataccgctt tttgaggtgt cgatcaaatt cagatttttc aaaagatctg tttctgttcg
     7081 caatactcgt tctgtgatcc ccatactttc cgataaactt cgacgtccaa tcggctgcat
     7141 ccaatagata tttcgtaaaa ttttatagcg ttcttgcaac acttcgataa tatctggtgc
     7201 aacagattca atcattttta tttcatccag catagcgatc tccttagttg gacactttac
     7261 gtccaatgta gacttaaaac gacccatata cgataaaaaa agaaattaac tattcgaatt
     7321 tcacatcaat aaagggaaat ctcaacctca cgactgtaag tctaacagtt ttgctctcgt
     7381 ttttcaacta aaaagactag aaaaagtgaa atactttttc tagtcttttg actgattagt
     7441 tatcataacg ttttcttttt gatgaactcg ggattcctaa taaatcacga tattttgcta
     7501 ctgttcgtct tgagatcgct atctcatctt ttttcagcaa ttcaaccaat ttctggtcag
     7561 acaacggctt gtttttgtct tcttgatcga ctaattcctg cagctttttc ttcgctgtat
     7621 ctgctgaaag gtcttctgtc tgttctgtac tattcgtagg aattcgtgta gtgaaaaatt
     7681 tttttaattc aaatacaccg aagtctgttt ccaaatattt cccgttgact gcacgactga
     7741 ccgtggattc atgcacatct atctcagttg caacttcctt taacgtaaga ggcttgattg
     7801 gacgttcctt gtcgataaag aaagcttttt gccgggaaac aatgacttga gcaacccgca
     7861 aaattgtatc cccacgttgt aggatcgttt tcttcaacca ttcgaattcc tgtttccgtt
     7921 cttttaaata attttcagtt tctttatctg cttgttgtga catttgtttg aagtaactct
     7981 cttgaaagcg tatatttggc tgattttttc ggtttgagat aacttgtaca tttttatcgt
     8041 cgtccactaa tacccggaca tcaggaataa tgtacaattc gctgctacgg tcgaaaatcc
     8101 ggccagggct tggactcaac gtttgtaaat agtcgaatat ctgctgaaca gcatgtagat
     8161 caacagaaaa acgttgtgcg atttctttcc attttcgctc caccagttcc tcaaaacatt
     8221 cttccaacac gatataagct aattccggcg cataatcatc tcgttctgtt tgaagcatga
     8281 gacattcttg caaactacgt gcacctactc cagctggttc taattgctga atcagagtca
     8341 acgcatcgag catttggata ggcgtagctc caatctcttt tccggcttct tctaaactaa
     8401 tcgtcaaaaa tccgttcaaa tcgatgtatt ctacaagata aagaacgatt ttcctcaaaa
     8461 aagtatctcg gtaattcaaa tggacttgtt cgatcaatga atcaaataaa gacccttttg
     8521 tatcaggaat ctgatttagg taatttgtct cttctccttc ataagaagat gaagaagctt
     8581 ttatgtaatc tggctgccat tccggctcaa caacttccac caatggattt tccattgctt
     8641 gattctcaac gaatgccaac agctcctcac tgttatattg taaaacttgg atggactgtt
     8701 gcaactgttg cgtcatggct agtttctgtg tctgctgctg tttttgtgaa aaattttgct
     8761 caaatttcat ttttttgtcc aatccccttg taatttttta tctgtttagt tacaattatc
     8821 ccagtgcttg cgtccttagt gtagtggata tcacgtaaga ttccggttct tgagacgggg
     8881 gttcgattcc ctcaggacgc gttctactgc tattttagca taatttacga atagttgctt
     8941 ctttattttt aagtgaaaac tgtccgccaa agaaaattgg tggacagttt tttgttgtac
     9001 ctgtatagat attgttaagc ctttgcaagt aaggtagtta tgtgttcaac tacttattaa
     9061 aatgtgttat ttattaaaga tacttgtctc acttactgaa ctggccgatg atcgtttctg
     9121 ccataatagt tctcgacagc cgttggatgc cctaataaat ccccaactgt cgtgatgcga
     9181 tacccttgct ctttcagata atcgatcact tgaggaacgg cacgaatcgt ttcaggatag
     9241 atatcatgga ataaaataat tgccccatcg tatactgttg cttggatctt ctgcaaaatc
     9301 aaatcaggat ttcttgtctt ccaatcttct gaatcaacag accattcgat gatcggtcgc
     9361 tggatgatcg ttgctcctac attcgtgaca gaaccatagg gcggacgaac aaaagtgggg
     9421 gttttgccag tgactttttt gatctcagtg tctgctttcg tcacttcctc cgtcatcgac
     9481 tgggcagaaa gagtggccag attctgatga tcgtatgtgt gattgccaat ctcgtgccct
     9541 gcttcagctg tctgcttcac gatttcagga tgctgcgccg tattttctcc taagacgaaa
     9601 aaagtggcat gcacgttttt ttcttttaag attcgcagaa gctgcggtgt tgtggtcgct
     9661 cctggtccat catcaaagct gattgcgacg cttttgacag ccggatctcg gctcgttttt
     9721 gtactgtctt gtgttgattg tgatgactga ctttgtacct tttccgctga ttcttgaatg
     9781 gcttctttga cgttagctgt ttcagttgat tggcttttaa tggttgattg ttctgattgt
     9841 ttgaaagatt gtgacgaatg ctccgaagag tgtgtttgtc cttttacttc ggtgatcaag
     9901 gaggccaccg aagtaatttc gaaaaacaaa agcacggata aagcaataat actgataact
     9961 aaataatttc ttttcactat atctcttctt tctctttttc tcttcttatt atttacttgt
    10021 acttacttga aaattgaaac tgctagtcgc aactacttct cgtctaatga tccaaataat
    10081 cttccgatgc caaaatcgtt tttgtcacgg acttaggaat gacaaattct ggtgtaccca
    10141 tataaccagg agcaatttcg tattgagaaa aagtcaaaac taattgatgc tctttgttca
    10201 aataaaaact tcgtgtaggc gtaacaagtg ttggtttttc tatcgtccct tcttgaatat
    10261 cttgctcagt ccaatagtac ttagatggat cttccttgac ttgttcggcc atctgccgtt
    10321 tgacttcttt tgtcaaaaca gatagatagg tgtcatcttt gaataaaagt ggaagtgaga
    10381 gctggacact gtttttcttg tcgattgtgt cgtatctttt ttcaacatgg ctatctgctg
    10441 ctgtttgggt gatctgcctt tcaatcacga gaaaccggtt atcgtctacg attttctgat
    10501 aatctcccaa aacttggtaa tgatccgttt caaaatttcc atattctttt tcagcttttt
    10561 caaaatctgc ttgtccttcg cgaaagtatt tttgatttaa tccatgaatt gttttatttt
    10621 ctttctcatt ttgtgtattg atcttcggta cctgaatatc gattttttca gatcgatcag
    10681 taaagcgttc gcctaagatc aattcaatga ctgatcctaa gatgggtaaa tcttctgcaa
    10741 agcttcgaac ttgactatta aaaagaaggc tacttgttgt aatcattacg atagccaaag
    10801 atactccgaa cccttggaga cgtctctttc tccgctttgc ttttataaat gattgctctt
    10861 cgataataaa acgctggcgg atctcctctt taagcccttt gggaattgct tgttctttat
    10921 aagttttctt tagttttttt aaattttctt tttgggaata catggcactc ttctctcctt
    10981 cattctagga tttgacgtaa agcattcaat gagcggtaca ttttcgtttt aactgtgttt
    11041 tcattcatat ccaagatagt cgcaatttct ttcaaacgaa atccttcaaa aaagtagagg
    11101 atcagaatct ctttttccgg actgtctatt ttatctaaaa tcatatggag ctctgcaacg
    11161 gaagtttcta aaggtaactg ttgggggata tcttgaattt caaataagtc cacaggcgtc
    11221 cttttttgtc tgcgccaact atctaatgcc gtgcggatca atatccgata aaaccatgca
    11281 ctaaaatgct cgatctcttt ttctttttca aaatagttca gggctttatg aaaactatct
    11341 tgcatcacat ctaaggcctc ttctttatca tgtacgtaat tcatcgtgat acggaacatt
    11401 ttttgataat cagtttcgat gagtccattc agcaggtgac ggtatttttc gatctgccgt
    11461 tttttcttat atccagtaaa aatcattctc cactcctttc tgcctcactt aatatgacgt
    11521 acaaggtgcg ctaaaagttt caattttttt attttttatg tatttacttg ggatttttat
    11581 atacaaaaaa gaaggtgtga caaaaatcgt gtcacacctc cttattctga taggctgatc
    11641 gtctctgtca caacctctcg taagaaaata atgtttattc tttttcagag tcagaaaatt
    11701 cttcctcttc ataatcttca tcatcatcgt cttcgtctat gatacttaaa tcttcttcga
    11761 tatttcctgg caagtcttct tcatcatctg tcgggtcatc tgctccgatt tcttgcaagt
    11821 cagaattata agcagcgatt tcttcatctt catcatcatc gtcatcaaac agatcatcgt
    11881 catcttcttc tgtcagatct gtatcttctg ggtcatcgtc attgtaatcg atcgcgtctt
    11941 catcatcatt gataaaggcg ttgactttct tgcgtttacg gcgacgtgga gtatcttcat
    12001 cctcttcgtc cattccatgg tttacttctt catcgatcga atcgatagca taccatgaac
    12061 gcaagcccca acgattatcc cctaatgaga taaagctgcc gtcaatattc aagtcagtat
    12121 aaaattgtgc gagtgaatca cgaatttcac tgtctgattt cccaagatag ttttggatct
    12181 ggttagctaa atcagaaaaa tccatgacat cgccacgttg ttctaagatc gcgtgtgcta
    12241 cttcgatcat tgataattcg cttttgtttg caccttcaaa tacactaatt tccaatcagg
    12301 acacgtcctt tcacagtcta ctccttatca tacaaaatca gacacaaaaa atcaaacttt
    12361 tcttttatat ttacgctgta aaatccaagg tcaaataata ttcgccgatt ttttccggtc
    12421 ccatgaacag atcataatcg attcgaacct tccctgctgt gggacggtct ttcaaactaa
    12481 catgcagttc tttcgtaaaa gtagcaatct ggatcatacc gtatggtgtt cgataggatg
    12541 tttctaaccg ttctttatag ccaaatttca aacgcatccg catttctcct gcacggatca
    12601 gctgtacatg accatctgga actaatttga tcgttacagg aatctcatcg cccgtctcct
    12661 cctgaatctc tttataacgt atgtacagcg tatcgccaat tgtcaccatt tgccctttca
    12721 aatcaaaata aaaatcttgc acttcccctt gctgttttac ttttgtacgc aatttgattg
    12781 aaatcggtgt tccttgtgat aattccatta ttcattctcc tttacttctc atagctacat
    12841 tgttcctagt ttcttttgtt ttgtcaacca acttgtccat ttttacagaa aacctttttt
    12901 ccaaaacaac tctttgattt tagagtataa tattgtagaa aatatgaatt tatacaaagg
    12961 agccggttga atgaaccctg cgattcgact agatcagcac atttatcaaa aaaacgactt
    13021 ttcccctttt gcattaacag atatattaac ggaatattct aaagaacacc agcaaatctt
    13081 tttacatttc tggcaatatg actgcacagt catcttaggt atgaaggatg ttcgaactcc
    13141 ttttctaagt gacgggatcc aaacactgaa agatgcaggt tacgctccag tgatcagaaa
    13201 ttcaggtgga ttagggatcg tatcagatga aggaattttg aatatttctt tgattttccc
    13261 ccaaagtgac acaaaaaaaa taaccataga tgacgcttac gagcaaatgc tcctattgac
    13321 taaatacgct tttccaaagg caccgataca agcattcgaa atcacccatt cttattgtcc
    13381 tggaacttat gatttaagca tccatggcaa aaaatttgcc ggtatcgctc agcgtcggat
    13441 caaaaatggg atttctgtta tgatgtactt aagtgtcaac ggtaatcagg aaaaacgcgg
    13501 gcaagtcatg aagcgatttt atcaatctgc tttaaaagaa gactttggta caaatggcta
    13561 tcctgcagtg gatcctgcta caatggcaac acttgaagaa ctgcttgacc aacccctttt
    13621 tgtaaaagaa gtcaaagaag catttgcaaa agcttttgaa aagctgtatt ctgcaagtga
    13681 tttacaggat tctaccgact ggctaaaaaa agaaatccag ctggagcaat ggaacaacca
    13741 aatccagaga atggaacaac gtaataacat aaaggagcta cattatgaca accccttata
    13801 aatatcaaat gctgcctatg gaaaaagttt ttcgtgatcc agtccataac tatatccatg
    13861 tccagcatca agtaatctta gatttgatca attcgaaaga agtccaacgt ctacgacgca
    13921 ttaaacaatt aggcacttct tctttcactt ttcacggagc agaacatagc cgttttactc
    13981 attcattagg cgtttatgag atttcaagac gaatctgcga tatcttttct cgaaattttt
    14041 caaaagaaaa aataggcaac ggaggctggg atgatcagga acggctggtg actttatgtg
    14101 cggcactgtt gcatgatgtc ggtcatggtc cttactctca tacatttgaa catatcttcc
    14161 atacgaatca tgaagccatc actgtagaga tcatcacttc tccagaaaca gaagtgtatc
    14221 agattcttaa ccgagtagaa gaaggttttc ctgaaaaggt agctagtgtg atccaaaaaa
    14281 cttaccccaa cccacaagtt gtccaaatga tttctagtca gatcgatgca gatcgaatgg
    14341 actatttgct tcgggatgct tatttcactg gaacagaata tggaacattt gatcttaccc
    14401 gaatattacg tgtgattcgt ccttatgaag gcgggatcgc tttttccatg agtggcatgc
    14461 atgcagtgga agattatatc gttagccgat atcaaatgta tgtgcaagtg tacttccatc
    14521 ctgtttcccg tggtatggaa gtaattttgg agcatctgct tcatcgcgca cacgagttgt
    14581 atcaagaaga tgccagcagt tttgaactcc attctcaatt acttctgcca tttcttgacg
    14641 gaagctttac tttagaagaa tacctcaaac ttgatgatgg ggtcttgggc acttacttta
    14701 ctcaatggtc tgaatctgct gatcctattt taaatgattt agctaaacgt tttttgaatc
    14761 gtaaaccact gaaatctgct actttttctg gcaaccggga ttcaaaatta gtccaagaac
    14821 tcactttgtt agtagaaaag gtcggatata atcctgttta ttacactgct gtgaattcta
    14881 gctacgatct accttatgat ttttatcgcc cagaacaagg cagacatcgc acacaaatcg
    14941 agattttgcg aaatgacgga actttgatag aattatcaca ggttagtcag cttgttgctg
    15001 cattagcggg acaagaacaa ggagatgagc gatttttctt tccaaaagaa atggttgatc
    15061 cgtccttgcg tgatcattat gatttatttg acgaaactta tcaagaattt gccagccata
    15121 tccgtaatgg cgcattgatt gaaataaatt aaaatatatt ggaggaaatt atgtctatca
    15181 aactagtcgc tatcgacatc gatggtactt tattaaatga gaaaagagaa gtaacaaaag
    15241 aagtaaaaga agcgattgcc gctgctgttg caaaaggtgt ttcgatcgtt ttatgcactg
    15301 gccgtccctt accaggtgtc caggaacagt taaacgagtt gaaccttttt caagataatg
    15361 attacgttat tacttataac ggggcactcg tacaacaaac gaaatcaggt aaaatcatcg
    15421 cccgtcacgg actgacacat gaagatttcc tagagatcga agtgatggca cgccgtgtcg
    15481 gcagtcatct ccattcgatc gatgacaaag cgatctacac gcctaatcga gatatcagcg
    15541 cttatacgat ccacgaagca agtttagtca agatgccgct aaaatatcga acacctgaag
    15601 aaatgacacc agacatgaac atcgtcaaaa tgatgatgat cgacgaacca gaaattttag
    15661 atgcagcaat tgcgcgttta ccgcaaactt tccgtgataa atacacaacg gtcaaaagtg
    15721 cgccctatta cttcgaagta ctaaataaag aagcaagtaa aggtgcggca gttgcgaatc
    15781 tcgcacagca ccttggtatt gatcaagatg aaatcatggc tatcggagat aacgagaacg
    15841 acttatccat gattgaatat gctggtctag gagtagcaat gggaaatgcc gtttctcttg
    15901 tcaaagaagc agcaaatgtc atcacttcat caaacgacga gcacggtgta gctgaagcaa
    15961 tcaaaaaata tgtactgtaa taaataaaaa aactgagaga aagctgatct actcgctttc
    16021 tttcagtttt tatttttttc gatccatctc tcgactgccc atcggtggct accacgtttc
    16081 aatttcacaa gcgcttcttg agggctcacc caccaaattt tattggtctt ttcagttggt
    16141 tctcctatac gcttccaaga atcagcaaca aagaaatatc ctggattata ataataagtt
    16201 gcacgataat tcgagtaaaa atactcatca gcttctccca gataagaaga aatttctgcc
    16261 acaattccca tttcttctat cagctcacgg acaatcgctt cttctttcgt ctcttctcct
    16321 tcaatttccc cgccaggaag aaaaaaagca ccatttggag cctcgattac tgcaatctgc
    16381 tgatctcttt ctatgacaat atatgctgca taacgggttt gataatcagc aaattgcttt
    16441 ttttcaccaa aaattggttt atccatgaat agtttccacc ttaacagttt tattttctat
    16501 ccattttaca tgagaaattt ctcatgaaca agcacaaatc aactatttcc ttacttcttt
    16561 ttgttatgat tgagtaaaag gaaaaggaga gatgaatcta tgaaaatggc tcacacatgt
    16621 gttcgagtga aagatcttga agcatcaatc gaattttatc aaaaggcatt tggttttgaa
    16681 gaaagccgcc gccgtgattt tccagaaaac aaatttacac ttgtttattt gacacttcct
    16741 ggggatgact atgaattaga gttgacttac aactatgatc atggtgcgta tgatctagga
    16801 gacggctatg gtcatatcgc tatagcagct gatgatctag aaaaattaca cgagcaacat
    16861 aaagctgctg gtcttgagat tactgactta aaaggattac ctggcactgc gccttcttac
    16921 tactttgtca ttgaccctga tggttataaa atcgaagtca tccgaggata gttagtatgg
    16981 ggcattttac tcttgattga ataaaaaaca aacaccaata attaaaatga gacgcaaact
    17041 tttcattaaa ttgaaaaagt ttgcgtcttt ttttaacaac cattgtagct ataaggttgc
    17101 cactctccac caatttattt ttaattcatt tgaaagaaat caaataaacg aggtaccttt
    17161 agaaaacaaa actgcttttc ctcctattat cacacgatct tcttttaatt cacattctag
    17221 atccccgccc cttgatgaca tttgataagc tgacaatttt gttttattca ttctttctgc
    17281 ccaataagga ataagattag aatgtgctga tccacatact ggatcttcat tgattgttaa
    17341 ttttggaaag aaggttctag aaacaaagtc tttttgcaca ctttctgctg tcacgatgac
    17401 tcctattcct aattcaaaat ttttgatagc tgtaaaatct ggctgcaatt tagccaccgt
    17461 tttttcatct tctaatacga aaaataaatc acgtgctaag taggcttctt taatcttagc
    17521 cccaatcgct tcttcatatt cagacaatat cggcactttt ttcggcataa tactaggaaa
    17581 atccatgtaa taataatctt taattcttgt cacaaataga ttaccgcttt gagtagaaaa
    17641 tttaatcgtt tcatcaggta ttttgtaata gttaaataaa acaaatgctg ttgctaatgt
    17701 cgcgtgacca caaagatcga tttctcgatc tggcgtaaac catctaagct caaattcttg
    17761 gtttttcttc actgtaaatg ctgtttcaga taaattgttt tcaatggcaa ttttttgcat
    17821 cgtaccttct ggcagccatt cttctagcac ataaaccgct gctggatttc ctttgaacac
    17881 ctcatctgca aatgcatcaa ctacgtaata atctaagttc attttaaagc ccctttctct
    17941 aatttgttat aaatatattc tgcacaacaa agatcttcaa tagctagacc aactgcgtcg
    18001 aaaatcgtcg gcctcgattc tcctttataa aatgtttttt tctcagaaac taattcgcct
    18061 aatgtaccaa caatacaaga ttcctctaaa tctccttctg ccatggctat aagcaggtcc
    18121 ccgctttctg ttaaggcagc tgagtaatca tctacataga tttgagaagt ttccatcaac
    18181 gatgtactta tctctcttgt atcaggagta aatgtgccca cagcattgat atgtgcgtct
    18241 tcccttaccc actctttttg aagaaatgcc tctttactag aggtcaacgt acaaatgatt
    18301 tccgctggac gtactgcatc ttctactgat attgcattta taaacgttat tttaggatac
    18361 tgttttttag ctttagcgat aaaagtattt cgtctttctt ctaaaatatc gtaaacaaac
    18421 actgtagtga tgggacgaat ggtagaaatc gcagtcaaat gcgacaatgc ttgttgacca
    18481 gctccaatta aagcaagtgt ttctgcatgc ggatttgcta agtaatctgt tgccagcgca
    18541 gatacagcag ctgttcgaat ccaggtgact gcatttgcgt ttatcaatgc tttgggtagt
    18601 ccgttttgag catcaaacaa gaaaatctct cctaaatgag aaggtaaatg atactgatga
    18661 ttatccggaa aagcggtaat gacttttgtg ccaaaatatc tgttctcccc caaataagca
    18721 ggcatcatcg caaacatatt tttgcctttt ccgtcaggta aaatttccac caatcgagga
    18781 ttttggctga ttttaccact ttcataatca aaaaaacatt ttttcattac ttgaatcgct
    18841 tcttcaaaac taaaagaagc tactacttcc tcaaaattaa taattttcat agacaattcc
    18901 ctcctcacaa aagattaact tctaaaatca ata
//
LOCUS       DANKHL010000037        18884 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000037 DANKHL010000000
VERSION     DANKHL010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.039"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(146..1111)
                     /locus_tag="PW101_000904"
     CDS             complement(146..1111)
                     /locus_tag="PW101_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDL2513324.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1345..2190
                     /locus_tag="PW101_000905"
     CDS             1345..2190
                     /locus_tag="PW101_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2513325.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2381..3568
                     /locus_tag="PW101_000906"
     CDS             2381..3568
                     /locus_tag="PW101_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL2513326.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3574..3930
                     /locus_tag="PW101_000907"
     CDS             3574..3930
                     /locus_tag="PW101_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDL2513327.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3932..4270
                     /locus_tag="PW101_000908"
     CDS             3932..4270
                     /locus_tag="PW101_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDL2513328.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    4346..4602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4665..5699
                     /locus_tag="PW101_000909"
     CDS             4665..5699
                     /locus_tag="PW101_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2513329.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5692..6378
                     /locus_tag="PW101_000910"
     CDS             5692..6378
                     /locus_tag="PW101_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2513330.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6402..7250
                     /locus_tag="PW101_000911"
     CDS             6402..7250
                     /locus_tag="PW101_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL2513331.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            7447..8220
                     /gene="sufC"
                     /locus_tag="PW101_000912"
     CDS             7447..8220
                     /gene="sufC"
                     /locus_tag="PW101_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDL2513332.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            8233..9519
                     /gene="sufD"
                     /locus_tag="PW101_000913"
     CDS             8233..9519
                     /gene="sufD"
                     /locus_tag="PW101_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDL2513333.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            9516..10751
                     /locus_tag="PW101_000914"
     CDS             9516..10751
                     /locus_tag="PW101_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2513334.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            10738..11208
                     /locus_tag="PW101_000915"
     CDS             10738..11208
                     /locus_tag="PW101_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDL2513335.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            11213..12604
                     /gene="sufB"
                     /locus_tag="PW101_000916"
     CDS             11213..12604
                     /gene="sufB"
                     /locus_tag="PW101_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDL2513336.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(12699..13841)
                     /locus_tag="PW101_000917"
     CDS             complement(12699..13841)
                     /locus_tag="PW101_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2513337.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(13880..14614)
                     /locus_tag="PW101_000918"
     CDS             complement(13880..14614)
                     /locus_tag="PW101_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2513338.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            14846..15121
                     /locus_tag="PW101_000919"
     CDS             14846..15121
                     /locus_tag="PW101_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2513339.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            15219..17216
                     /locus_tag="PW101_000920"
     CDS             15219..17216
                     /locus_tag="PW101_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513340.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(17292..17627)
                     /locus_tag="PW101_000921"
     CDS             complement(17292..17627)
                     /locus_tag="PW101_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2513341.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            17755..17969
                     /locus_tag="PW101_000922"
                     /pseudo
     CDS             17755..17969
                     /locus_tag="PW101_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            18006..18368
                     /locus_tag="PW101_000923"
     CDS             18006..18368
                     /locus_tag="PW101_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HDL2513342.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            18414..18740
                     /locus_tag="PW101_000924"
     CDS             18414..18740
                     /locus_tag="PW101_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513343.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT         6256 a         3263 c         3943 g         5422 t
ORIGIN      
        1 ttacgacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccag
       61 tacaaaaaca tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct
      121 ttttcacaac ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt
      181 tgctatgttc tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac
      241 ttgcaaacag acattccgca acttttaaga aatccaatac gctactatag atacctaaac
      301 tgttgaagta atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa
      361 aggctgcata gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa
      421 cgaaatacaa tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag
      481 caaagttttt aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca
      541 gcggacgtgt cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca
      601 ataagaacgc atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga
      661 agttcccggc agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat
      721 aaattgcttc aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa
      781 ggatcacttg gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt
      841 catatgaaac aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg
      901 aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa
      961 caaaaatgaa tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat
     1021 ggtgatagtc tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg
     1081 ggatcggaat aattttcaaa aagttcttca tatataatat tctccttttt atttaataga
     1141 ttttagtacc aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca
     1201 agcagacagc tgattgtctt catctaagtt actagttgga tcagcaactg ttttgttgta
     1261 catttctatt atagatggtt ttttgtatat ttaaatcaaa tagtttttat agtaattatc
     1321 ataaaaagtt atggaagtgg tgccatgttt aagttattaa cgacttttcg agcagtatat
     1381 gaaacaagaa atttttccag agcagcagaa gtactgtttt tatctcaacc agcagtctcg
     1441 aatcaaatca aacagttgga gcgggaattg aatatcgatc tttttcaacg taacggaaga
     1501 aaagagatgc ttccaactaa gcaagccgac ttattatatg aacggacact ggttttgtta
     1561 gaagaatggg aagaaactca gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt
     1621 cggatcgttg cttcccacac ctttgctgtc tatcttttgc ctcagttgat gaaacatctg
     1681 atcaaaaggt ttcctaaagt gcattttgag atcgaaatca cgaattctca tgaatcatta
     1741 gaacaagtag cgaaacatga atccgatttt ggttttatcg aaaagccatt agagacttcc
     1801 ggcgtacaaa gatactcttt gatggaagac cagctagtca tcgctgggga cccaaaaagc
     1861 aaattgtggc ttgtaagaga agcaaactct ggtgtttttc attacacgca gcgttatttt
     1921 gaagagatga atatccaaga taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg
     1981 ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg
     2041 ccaatgactc cgttgaattt ccatcgatat ttttatttca tccaacgaac caatctggtt
     2101 tctgatcagc tgctagagat tgccgaagag atccttcgat acaatgaaag cacagaaact
     2161 tctcacagcg aggagaatct cagctggtag ataattgcat gtatcaaaaa aagttctatg
     2221 atacactaaa gataagcagt aggcttttgt actgcatttg ctcaagcaac tgcacaaacg
     2281 gaaagttaat ttttcccaaa tgtctatgga aagactttcc attatctaac tggaccgcta
     2341 taataatcag cagtatggtt tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc
     2401 ttcgaacgat aatcgtattg attatggcgt gattttacca gtctttctgt tatgtttgat
     2461 tggtctgctc tcattatatg tagcacttag ccatgatccg aacaatccag cagtcttgaa
     2521 aggcgtaggg atgcaagttg tctggtattt agttggagcg gcagcaatcg ttgtcatcat
     2581 gcatttgaat tccaaatgga tctggaaact gactccttac ctttatggtt tggggttagt
     2641 ggtaatggga ctcttgctca aattctatga tcagaattta gctgcttcaa cagggtctaa
     2701 aaactggttt cgattcggtg cgtttacttt ccagccagca gagttgatga agattgcgta
     2761 catcttgatg atggcactga tcgtaactaa acataatacg caagtaaaag aacggacgat
     2821 gaaatcggac ttttggctga ttgggaagct cttgatcgtg acaataccgg ttttagcatt
     2881 gatcatggcc caagatgact ttggaacgat gcttgtcttt cttgcgatct ttggcgggat
     2941 tttcctgatg tctggaatct cttggcgcat catcgcacct gtagtaatct tggcagtagt
     3001 cgttggagca ggaacgatct tcttggttac gactgaaggc ggaagagact tgctttataa
     3061 agttggattc aaatcttatc agtttgcacg tattgattca tggctggacc ctttccatga
     3121 tacgtcagga atgagttatc agccggccca aggacttttg gcaattggta ccggtggctt
     3181 gtttggtaaa ggattcaacg taagcaatat ctatgtgcct gttcgtgagt ccgatatgat
     3241 ttttaccgtt atcggtgaaa acttcggctt tatcggtggt gcattcgtca ttttcttgta
     3301 ttttatttta atctatcgta tgattcgggt gtgtttcgat acgaataatg aattttacgc
     3361 ctatatcgca agcggtttga tcatgatgct gctgttccat gtctttgaga atattggagc
     3421 aaatatcgga ttacttcctt taacagggat cccattgcct ttcattagtc agggaggatc
     3481 ctcgatttta gggaacatga tcggtatcgg gttgatatta tcgatgagat accaaaatga
     3541 agcacctgca agatcttcaa ggaggtaaga aaattgtaca ccttttactg gtacccgaaa
     3601 tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc atggcgttag ttatcaaacc
     3661 gttgatatga tcaaagaagt accgaaggcc tcaacgattg agacttggct agaaacgagt
     3721 caagtaccaa tccgtcgcct gttcaacaca agcgggatga aatatcggga acttggactg
     3781 aaagatcaag taggcgagtt tacggcttca aaagcgagcc aagtcttatc gacagatggt
     3841 atgctgatca agcgaccact gatcttacga gaggaccagc ttgttgcaat tggattcaat
     3901 gaaaaaattt atgaaggaga actgatataa aatggcaggc aagcatttga agaaaaagga
     3961 caacctttgg atcttattta atggaaaaga atactgtgtt ggtttgacaa atgaagcgca
     4021 agaagaactt ggagacatca cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa
     4081 agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct
     4141 aacgggtaca gtttcttctg ttaatgataa aatcggtgaa gatatcaata tcttgaatga
     4201 ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat gttgatccaa aagaatttga
     4261 cgaattataa aatcagaaaa tcacttgaaa aaagttgaca ctgattcttc cagttgctat
     4321 aatcataagc aatcctatat gaaacgagaa agctttgaaa agaagagtac atgtcacgga
     4381 cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa aatgagatgg aagatggtct
     4441 tagagcagag taagcgagcg taagcaaact tactacggga gtgcccgtta cagcgcgaca
     4501 gtatacggaa agaagctaag tccaagtact ggaagaggct gttattgcga agtaacggta
     4561 aatcaaggtg gtaacacgga agttcaagct ttcgtccttt tgcttaaggc aagagggcga
     4621 aggctttttt gtttgaaaaa ttataagaga aagatgtgaa tggcatgtca ttaatccaac
     4681 taacgaatgt caaaaagaca ttcaatggga aaaacggaaa catccaagcc gtcaatgacg
     4741 tttcactaaa cgttgaaaaa ggggatattt atggaatcgt cggctattct ggcgcaggaa
     4801 aaagtacatt agtccgtctc ctgaatggac ttgagcttcc cacgagcggc gaagtgatcg
     4861 taaacgatca agatattaca aaattaaaaa acaaagaatt acgtacattc cgaaagaaaa
     4921 tcggtatgat cttccagcat ttcaacctgt tatggtcaag aactgtactt gaaaatatcc
     4981 agctgccgct ggaattagct ggcgtaccaa agagcaaacg taaagaacgg gcagaagaac
     5041 tgttgcgttt agtcgggtta gaaggacgcg gacaagcata tccttcacaa ctttccggcg
     5101 gacagaaaca acgcgtcggt atcgcacgag cattggcaaa tgatcctgag atcctgcttt
     5161 gtgatgaggc tactagcgca ttggacccgc aaacaacaga agaagtatta gacttattgt
     5221 tagcgatcaa taagaaattg aatttgacca tcgtcttgat cacacatgaa atgaatgtga
     5281 tccgcaaaat ctgcaacaaa gtcgctgtga tggagttggg gaaagtcgta gaagaaggag
     5341 acgtattgac tgtcttcaga caaccgaaaa aagaagtaac caaacgcttt gtacaacaag
     5401 acatcgaacc agaagctgat tcgacagaac tattagctga actcatcaaa gaaaatccaa
     5461 gcggccgttt ggcaactttg acctttagcg aaagaaatgc caacgagcca gtcatttccc
     5521 aagcgattcg acgctataac atcgatgtca acgttgtgta tggaaagatc aaacaagtga
     5581 aagaaggttc ctttggttca ttgactgtct tgatgactgg tgaaaccaaa gaattagatc
     5641 aagcagaagt gttcatcaaa gaacaaggag taggaattga ggtgatccat cgtggataaa
     5701 agcttttcag aaacatatct agattttaaa caaatcaacc cggaaacgat gaatcaggct
     5761 atccttgata cactatatat gacattaatc tcaatggtct tagtgacgat catcggatta
     5821 gttttgggat tgatccttta ctctattgga cgcaaaaaca aaccgtatgc tcgaatcatt
     5881 tatggaatcg tatcgatcat cagtaatatt ttccggtcaa cgccatttat gatcctgatg
     5941 gttttgatca ttccatttac taaagcaatc gtaggcacta tgctaggtgc gcaagcagcc
     6001 attccagctc ttgttttatc cgcagcacca ttttatgccc gtctagtaga gattgctttt
     6061 cgtgaagtca gccctggtgt actggaagca gcagatgcaa tgggagcaag ttactgggaa
     6121 atcatctgga aggtcttgat acctgaaagt attcccgcat tgatttctgg attaacagtt
     6181 acaacgatct cgatgatcgg atttactgcg atggccggcg cgatcggcgc aggtggactt
     6241 ggaggtcttg cttggcagga aggctaccag cgtggtaatc taacagtgac attcgttgcg
     6301 actttaatta ttttactaat cgtatttatc gttcaaggaa tcggtgattt cttaactaag
     6361 aaaacagaca aaagataata ggatataggg ggatgtagga tatgaaaaag aaaatttttg
     6421 gatttgcagc agcgcttttg ctgactgtag gattagcagc atgcggtaac aacagcgaca
     6481 gcaaagattc agcaaataaa gcagacgaca cgacattgaa agtcggcgct tcaccgacac
     6541 cgcacgcaga gattttggaa catgtgaaac cattattgaa agatgaagga atcgatcttg
     6601 aaatcgtcaa attcgatgac tatgtattac cgaaccaatc tttggaagaa ggcgacattg
     6661 atgccaatta cttccagcat attccttatt tgaacaaaga aatcaaagaa aaaggatatg
     6721 actttgtcaa tgccggtgct gtccacatcg aaccaatggg cttgtactct aaaaaagtca
     6781 aagatgtttc cgaattaaaa gatggtgcaa caatcattac ttcaaattct gaatcagact
     6841 ggggacgcat catcacaatt ctacaagatg ctggcttagt caaagtcaaa gatggagtag
     6901 atctggaaac agcaacattc gatgatattg aagaaaaccc taagaacttg aagttcaacc
     6961 atacgatcga cccatcactt ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg
     7021 caatcaacgc aaacttcgct tacggcgcag ggttgaatcc attagaagat gccgtattgc
     7081 ttgaaaagga caactctcct tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca
     7141 gtgacaaaat caaaaaactg attgaagtgc ttcaccgcaa agaagtccaa gaatggatca
     7201 cagacaaatg gggcggctct gttaaaccag taaaggctga tgcaaaataa ggtatggaac
     7261 aaaaaagatg agaaataagt ctgtaatggc ttatttctca tcttttttat acctattcta
     7321 atttagaaat caaaattgtt taataataaa taaaagatat ttgattttag taaggaaggt
     7381 cattgcgctt cattctcaat tagtctaaaa tgagtgagaa cgaaagagaa tttgggggta
     7441 taatgcatgt ctgtcttaga aataaaaaat ctacatgtgt cgatcgagga aaaagaaatc
     7501 cttaaaggcg taaacctaac aatgaaaacc ggagaaatcc atgccatcat gggacctaac
     7561 ggcacaggaa aatctacctt atctgccgct atcatgggca atccaaacta tgaagtaaca
     7621 gaaggcgaaa ttttgtttga cgggaaaaat gttttagact tggaagtaga tgaacgtgcg
     7681 cgacttggct tatttttagc tatgcaatat ccaagtgaga taccaggtat cacgaatgcg
     7741 gaattcatgc gtgcagcgat caatgcgaaa cgtgatgaaa acgacaaaat ttctgttatg
     7801 gcatttttga aaaagttaga tgaaaaaatg gctttactaa atatgccaga agaaatggct
     7861 gaacgttatc tgaacgaagg tttttctggc ggagaaaaga aacggaatga gatcctgcaa
     7921 ttgttgatgt tagaacctac atttgcaatt ttagacgaaa tcgattcagg tctggatatc
     7981 gatgctttga aagtcgttgc taaaggagta aatgaaatgc gcggagacaa tttcggtgca
     8041 ttgatcatca cccactatca acgattattg aactatatca cacctgatgt cgttcacatc
     8101 atgatggaag gccgagtagt catgacaggg aatgctgacc tagctaaacg tctggaagca
     8161 gaaggctacg ctggtatcag taaagaatta ggaatcgact ataaagaaga agaagcgtaa
     8221 gggggagcaa gaatgaaaga aaaaaactgg ctcgattatc ttgacgcggt aaatgatttt
     8281 tctttaagta aaggcgaacc tgattggatg agaaccttcc gtcaagacgc actagcgaaa
     8341 gcagatgaac tgccattgcc gcatatcgat cgggtgaaat tccatcgctg gtcgttattt
     8401 gatgtgaaag aaacacaaac catcagtgaa acaggcacga ttccggcatt tgatgcaatg
     8461 aaagacaatc ctgtgcttgt ccaacaaggt tcatggacga tttttgaaca actgcctgtg
     8521 gaattagcag aaaaaggagt catttttacc gacttgttca ctgcaatgat cgaatatcca
     8581 gaactagtcc aagaatatta tatgaaaaaa gcagtaaaca tgaacgaaga ccaattgact
     8641 gcactccatg tagcttttat gaacagcggt attttcctgt atgtgcctaa aaatgtcgtg
     8701 atcgatgaac cactcgagtc cttattcatt caagatggcg catccgatga gcacttcttc
     8761 aagcatgtgt tgatcgtggc agatgaacat agtgaatttt cttatctgga acgtttccag
     8821 acaaccaaag aacaagtagc aaaatcatca ggtaatatca tcgtggaagt catcgctaaa
     8881 gctggttcaa aaatcaaata ttcagccgta gatcaattag gagaaaatat tacttcttat
     8941 atgaatcgcc gtgggcacat tttacgagat gcttctgtcg actgggcaat tggtgtcatg
     9001 aatgacggtc atgtgatcgc tgactttgac tcagatttag caggagaagg tgcacacgca
     9061 gaagtcaaaa tcgtggcaat cagctcgggc agacaaatcc aaggaatcga tacacgagta
     9121 accaataaag cccctcatac gatcgggcat attttgcaac acggagtgat ccgagaaaaa
     9181 ggaacattga cctttaatgg tatcggccac atcttgaaag gggcaaaagg tgcagatgcc
     9241 caacaagaaa gtcgtgtctt gatgctttct gataaagcac gtggtgatgc gaatcctatt
     9301 cttttgatcg atgaaaacga agtaactgct ggacatgctg ccagtgtagg acgagttgat
     9361 ccagaagaaa tgtactatct gatgagtaga ggattgcata aagaagaagc agagcgctta
     9421 gtgattcgag gattcctcgg ttctgtctta acagcaatcc ctgtagaaca agttcggaaa
     9481 gaattagtcg aagtaatcga agggaagttg aatggatgat cgatgctaaa aagctacgca
     9541 atgattttcc tatcttattt caagacgtca atgatgaacc tttagtttat ctggataatg
     9601 cagcgacgac ccaaaagcca aaacaagtac tccaggcatt agcgtactat tacgaacacg
     9661 ataacgccaa tgttcatcgc ggtgtccata cgttagcaga acgcgcgacc catgattatg
     9721 aacaagcaag agaaaaagta cgggcgttca tccatgcaaa agaaacagca gaagtgctgt
     9781 ttactcgtgg tacgacgaca agcttgaatt gggtagccag aaccttcggc gaccgctata
     9841 tcaaagcagg agacgagata gtgctctctt acatggaaca tcattctaat gttatcccgt
     9901 ggcagcagtt ggcaaaacga acaggtgctg tcttgaaata tatcgaacta acagaagaag
     9961 gcttcctgga tatggaggat gcagcgaaaa agattacgga caaaaccaaa atcgtctcca
    10021 ttgctcatgt atccaatgtc ttaggtgtct tcaatcctgt tcaagaatta gctgaacttg
    10081 tccatgaaaa cggcggggtc atcgtagtag atggtgctca atctgccccg cacatggcca
    10141 ttgatgtcca agcactagac tgtgatttct ttgctttcag cggacacaaa atgtgcggac
    10201 caacaggtat tggtgtttta tatggtaaac gaaaatggtt agaagaaatg gaacctgttg
    10261 aatttggtgg cgaaatgatc gattttgtcg aattatacga cagtacatgg aaagaactac
    10321 cttggaaatt cgaagcaggg acaccaaata tcgccggcgc tatcgcatta ggccatgcaa
    10381 tcgattattt gcaaaaactc ggtatggaca atatccatcg atatgaagaa gaactagccg
    10441 cttatgtgtt gccaaaatta caagcaattg atggattgac tgtttatggt ccacaagacc
    10501 cgaattttcg gacaggagtc gttgcattca atttggatgg gttacatccc catgatgtcg
    10561 cgacagcttt agacatggaa ggggtagccg tcagagcagg tcatcattgt gcgcaaccgc
    10621 tattgaagta ccttgaagta tcagcgactg ctcgcgcgag cttttatttc tacaacacaa
    10681 cgcaagacgc cgaccgttta gttgaagcaa tcatggcaac aaaggagttt ttccaacatg
    10741 ggactatcta aattagacaa tttataccgt caagtgatct tggatcattc ttcccatccg
    10801 catcatcgtg gaagcttgac tgactcgaca agcaagatcg aattgaacaa ccccacatgt
    10861 ggagatgtga tcgaattgca agtcgatgta aaagacggaa tcatcaaaaa tatcgccttt
    10921 gacggcagtg gctgctccat cagtacggca agtgcctcga tgatgacaga cgccgtcatc
    10981 ggaaaatcaa tagaagaagc agaacattta actgtagact ttctacagct tgtccaagga
    11041 aaagaagtca aagatcccga ccaattaggc gatgctgcta tgcttggtgg tgtagctaaa
    11101 tttcccgctc ggatcaaatg tgcgacactt gcctggaaag cactaggaaa agcaatggaa
    11161 aacgatggta ccgcagcttt ccatcattac cacaaggaag aggagtgaga acatgggcgt
    11221 accagaatta gaagaatata aattcgggtt tcacgatgat gtcgaaccgg ttttcagtac
    11281 aggagaaggc ttgacagaag aagtgatccg tgagatgtca agaatcaaag gcgaaccaga
    11341 atggatgctt gaattccgtc tgaaatctct agaaacattt aacaaaatgc caatgcaaac
    11401 atggggcccc gacctttcag acatcgattt cgatgccatc aaatattacc aaaaaccaag
    11461 tgacaaaccc gctcgtgact gggaagacgt accagaaaaa atcaaagata catttgaaaa
    11521 gattggtata ccagaagctg aacgagctta tctagccggt gcttccgcac aatatgaatc
    11581 agaagtcgtg taccataata tgaaagatga attcgaaaag ttaggaatcg tctttactga
    11641 cactgactct gctttgaaag aataccctga attattcaaa gagtactttt caaaacttgt
    11701 accgccaacc gacaacaaat tagcagcgct gaattcagcc gtatggtcag gtgggacctt
    11761 tatttacgtg ccaaaaggcg tcaaagtcga tgttccatta caaacgtact tccggatcaa
    11821 tgcggaaaac acggggcaat tcgaacgtac gctgatcatt gttgacgaag gagcaagcat
    11881 ccactatgtc gaaggttgta cggcacctac ttattcaagt aacagcctgc atgccgccat
    11941 cgtagaaatc tttacaagaa aagatgcgta ttgccgttac acaacgatcc aaaactggtc
    12001 agacaatgtg tacaacttag taaccaaacg agcaaaagca tacgaaggcg caaccgttga
    12061 atggatcgac ggaaacctag gtgcaaaaac aacgatgaaa tacccaagtg tttacttaga
    12121 tggaaaaggt gcacgaggca ccatgctctc catcgccttt gcaggagcaa accaaatcca
    12181 agatacgggt gcaaaaatga tccacaatgc accaaataca tctagctcta tcgtctctaa
    12241 atcaatttca aaagacggcg gagaagtaaa ctatcgagga caagtaacct ttgccaaaaa
    12301 cagtgcgggt tcgatctctc atatcgaatg tgacacgatc atcatggacg aatggtcgaa
    12361 atccgatacg atcccattca atgagatcca caacagccaa gtctctttag aacacgaagc
    12421 caaagtctca aaaatctcag aagaacaact ctactatctg atgagccgag gcctgaccga
    12481 atctgaagca actgaaatga tcgtcatggg ctttgttgaa ccattcacca aagaacttcc
    12541 aatggaatat gcagtcgaac tcaatcgatt gatcagttat gagatggaag gcagtgttgg
    12601 gtaaacctgt tataggagct agaacccttg atatataagg gttctagctt ttttgttttg
    12661 cctgtatact agaaatgacc agattttgac cagctctttt actgatgagc catcatatca
    12721 gcaaagtctt gacctgtttt cttaggtgca ttatttgtaa tatgtgcata gatgtctaat
    12781 gtcactttac tattttcatg ccctaatcgt tgttgaacta ccttgacaga ttctcctgct
    12841 tctaataata aactagcatg tgtatgtcta aacccatgag gtgtaatacg aggcaagtta
    12901 tattttttca aaatatatct taaccaatca ttaacagtgt ttggaatgta tagagtgttt
    12961 ttaactgttg taaaaacatg ttgctcttta gagctagtat tgtatccaag ttttaaatac
    13021 tcttcttttt gctgtaattt ccagttgttg aggatagaaa gagtctcagt atctaaagag
    13081 ataactcgtc tagaagctct tgttttcgga gattgaatta taactttacc aaattcatct
    13141 acagcaagag ttttattgac atgtagttct ttattaaacg tgtctatgtc tttccactgt
    13201 aaagcgagta cttcactctt tctcatacct gtgtatgcca ataatctgaa gaaggcgtat
    13261 tgtttcatgt taccaaaatc tttaaaagca tccagtaaat tattaagctc ctcttttgag
    13321 taaaatttac gatttttctc atactctttt tttcgtggaa gaagtgtttt ttccatagga
    13381 ttattgtcaa tatattgcat tgcaaccccg tagcgtaaaa tttgtgcagt ctgttttctc
    13441 atataatcgt aactttcata tttactgtgc cattcatcta ccactttttg acaatatggg
    13501 acagagatat tcgatagttt aagcttacca aatgctggaa gtatttgatt atttgcgtat
    13561 cttactgaga ttgcgattgt tgaagcttta acactggtac gctgatgagt tatccattca
    13621 ttatacaggt cttcaaatgt catatcaggt gctttctttt taacaccgta taatatttga
    13681 tgtctgtact cgtcataatc tttttgagct tcttgtttgc tactaaaacc tcttctcgta
    13741 gtatttattt tttcaccagt tcgtggatcg gtggcaatgt gtcctttata catccacgcc
    13801 acttgaccgt ttttcttttt gtactttgat atttttgcca tgaaatcatc tcctcttagt
    13861 ttacaaggag tacttaccac tagatggtat cacctccctt catttttttc ttagtgtatt
    13921 ttttcccaat tgatgaaatc gtttgttcta gcttgtttat attatcagta atttctctca
    13981 cagcttcttc gggataagac atgtctttca attgaatagg tagccattca tctaccattc
    14041 tttctagtag ttcttctata ctgctagtga ggaaaagaat gttattgtca aattcttcta
    14101 caatagcatt gatgaataat tcttctattt ttaagatatt gaaaagacta attttttcat
    14161 ttttaacttt attacaagtc ttgttagcaa taaattcttg catctctggt tctaatttgg
    14221 aatatctatc cagccatgta tctaaattag agtgtactgg tgggaactgt tgacgaagat
    14281 gctggaacag gtttttatct ggaaatgtta taccatctag tacaatgcta tctctataaa
    14341 gaataattcc caaaatatat tcttctacat tgccacaaac taactcctct atactcatat
    14401 ttcccaaatc agcaattcgg gataatcgct ctggtattgg taaataggtt cccttctccc
    14461 attcgtagat agtttttttg tcaattaatt gaccgttgtt aatcataaga ccaaatgctg
    14521 gttgtgttaa ttttttactc tttcttatag cttttatttt tgttccgagt atcttttttc
    14581 gttctaaata atttattggt ggacgtttta tcatattgac cacacctcct aaaattagta
    14641 tacctcgaaa aaggggtgtt gtaaataaat tttacaaaga tattgacacc tcttttttat
    14701 cgaggtatta tgtgtatgtc gatgaactgg taatcatttt ttgtttgatt aatcggttaa
    14761 taacaaaata cttttatttt gagcaaaaaa tacgaagata attcttagaa aatttgttca
    14821 agcaatcacg aaaggagggg gaaatatgtc tctacaaatc attgatagca ttgtttctat
    14881 acaagttgaa caacaagtga aagagataat tacagtgcta aagcctatgc tagaagacat
    14941 tatttctgga gtcactctag agtatatgga ctatcagcag gcaagtgaat atctaggtgt
    15001 atcagtagga acgattagaa aatatgtttc tcaatacggg ttacctgtta taaaaattga
    15061 tacggtcatt agatttaaaa aatctgatat tgatagtttc atggaacggt ataaaggttg
    15121 agtaaacaaa taagactaaa acatatagta ataaagagtt tgtttttatt atctgtatgt
    15181 tttagtcttt aacagaattt tataaggagg aaacacaaat ggaaacatca atgattaata
    15241 ttccaattta tgtagataaa gaaaaattat tagagaagcc agttacaaaa aaagaaaaaa
    15301 ctgatgtaag agataggtgt ttagataact atagagaagt tacaagagat gagtttgtag
    15361 agatactaaa ttcatcacaa tcatttattc caagcaagac aaagaataaa ggcaatgaca
    15421 aggagagttt tgtggaaacc agagtaatta ttctggatgt cgataacact gttaaagatg
    15481 agaaggggaa ggtagtagat ttgagtaaag atgatagtag atacttaagc atagaaaagg
    15541 ttttatctat caatttagtt cataattctg cgtttgcgat acaaaaaagt attcggtact
    15601 ctgaaaatct agaaaaatac aagattgtct ttctattaaa agaagcaatt actgactatg
    15661 gtaaaatggt tgctgtttac gaatacttga aaaagaacat ccccgggtgt gatgacaatg
    15721 taaatgcttc gaatagaatg ttttttggag gatataagtc agatgcagtg ataataatta
    15781 atgaagacaa catgttagat attactgagc ttccgattga ttttaccatg acatgttcta
    15841 aaaattgtca aagtgattca gggagagagt atgaactagt caatgaaacc gattttgtta
    15901 agttgataaa aaatgacgac aaagaagaaa tgaaacagtg gtttaaagac tgcctgtttg
    15961 attcctccga catgtctttt aatgagattt atgaaagatt gttaaccatt gatatgaata
    16021 aattacttaa gtcaagtaaa aacttaagat gtctgtttca caacgatcat aatccctctg
    16081 ctagtatttt taccagtaaa actgggcatt ccatttttta ttgtcattcc agtaattgtg
    16141 atatcagtga aaattttatt ggagttgtga tgctaatttt aaagaaagac agtcgggtag
    16201 aaacatttaa ttggctatta gagaacctag acttgtatcc tacacatttt cgaaaattga
    16261 aagaggaatc aaaagtactt tttgacactc ttgaatcaat gaaagacaaa cttttcagga
    16321 caataaggaa ttatctagag gaaatgcgac gtgtctatga tgtcttgctt tctgatgtta
    16381 atttttttga tgatagtaag gaaagtgtga cttgtatttt gtcgggggaa caattagcta
    16441 aaaaaatgag tttttactat ggtaaaactt atgacataga taaatgtaat aagttactat
    16501 cacttatgac ttttatagga ttgataaaaa aattagatga tgaagatgtt cctttgaaga
    16561 tgctcgaata cctaaatagg ttaaaggaag agaaagcgtt aggaaacttt aatcataaga
    16621 gaagcaatgt ctatcgatta gacgttttta atgcaacttc tgtgattcaa aaattgaatg
    16681 aagatatcct tccacttctg gaaaaatttc attttacata tcaatatttt tcttatgaat
    16741 gggttaagat atgttttaac gaaaaagagg ctaaaagagt atttcctcaa aattcccgat
    16801 cagaacttag caaggaaaaa caattgattt tagatgaagc acatgaagtt ttacagggct
    16861 tatcgtggca aggaacatat ctaatgacag agaaagaact tactaacact gtttgtgaac
    16921 gtctacgtaa tgttgggaat aacagcgtgg aaaatgcttt aaaagaaaac agaggctact
    16981 ttgttaaaca aggattcact cttgaacgag cttctaagga tattaagaga ctatacaatg
    17041 tgaaagcaag tagtagtttt ttagtctacc tagcaaaagg agcagggata gatgaagtaa
    17101 taaaagaatt gatattatca ggagttgaat tagttcctct ttatcaattt acaagtgtaa
    17161 caagagatga tggacggaga gtaaggaaga gaaaagaccc aattgtatta aactagacag
    17221 gttatccata gctcttaatc ttatttcatc tcaatacacc caccgttaaa gttcggtggg
    17281 ttatttattt ttcaatcttt caatagctga ttgcgttcat tttcttgcct ttttaatagc
    17341 ttcatatagt caattgttcg aactgcatct tcagtgctaa gagaacgata gagagccaaa
    17401 aactcttcct cataatctac gacaacattg tctagaaaat ggcttacagg ggttcctagg
    17461 gctctagaaa gagcggttaa gctatcgagg ttaggctctg ttacagaatt ttcccatctt
    17521 gctactgcct gttgtgtaga gaagattttt tcagctaatt ctttttgtgt ccacccgaga
    17581 gcttttcgct tttctctaat gacctttgca tattgtttct gtttcattaa tacaccccca
    17641 tctcagtata gtacaacaag ttgttgtatt tgtaatggta tttgaagttt ttttatattt
    17701 tatgagttga aatacatcat attgttgtat ataataaaac aggaggggag aatcatgaaa
    17761 aaagtaagta atctcagccg atacagaaaa gaaaaaggac tttctcaaac ggagttagca
    17821 aaaaaatgaa tgttacacag caatgtgtaa gttcgtggca aacagggaga acgattccta
    17881 aaccctatca aatgaagatg ctttctgaga tattaagtgt accaatgaat gagctgtttt
    17941 ctgatgtatt taataaagta aacagttaac taataaacaa ttgaatgttt ttaggaggag
    18001 aagacatgta ttgtagaaaa tgtggcaaag aaataacaga tgaaagtaag tattgccagt
    18061 tttgcggagc actacaagaa acagatgaga caactggagc agacgcagta aagaaaagta
    18121 ctgagcagac aattaatgaa atgaaggaac aaatcgtaca tggacaagag acagggaagc
    18181 tgtatttgat tattggttgg atttctatgg tagtagcgtt agcatttatt cctgttgtat
    18241 ttggtgctgt tgctgttatt atgggctact tatatcgaga taaagatgaa caaatgggta
    18301 cgattttgat gatagcaggg gtagctggag caatctttgg tgtactttta gggatgtcaa
    18361 tgtactgata agagggataa gagcaaatta ttttggagat aggagataaa aagttgaata
    18421 aagtaaatat gaacaagaag tttatcatta tagttatcgt tgtcttggct attataggta
    18481 tcattttttc aaaaatgaca ggtacatctg gattagagaa gtcatcgaaa ccattggtaa
    18541 cacagatttt acaagaacag tacaacttaa atcgatcatg tgatgatgtg acgattactc
    18601 atgaaaatgg agataacaca taccgggcta aagctatatt agacaatgga agtgctatca
    18661 acataaatat tgagtactat ccaaagaaag accgtatata tgtggagata ccatatgctg
    18721 aggtgttaat gttaaattga tttaaatgta ttatgcatac cgtccaaaca ggaactccca
    18781 aaggtgtaaa ttcactaggg agtttttata gtttcctata aattaaatac taatacaggt
    18841 gacaaaaaaa tattacaatg attgtcggga atttttatgt tatt
//
LOCUS       DANKHL010000038        18686 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000038 DANKHL010000000
VERSION     DANKHL010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.040"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(71..982)
                     /locus_tag="PW101_000925"
     CDS             complement(71..982)
                     /locus_tag="PW101_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL2513344.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1359..1997)
                     /locus_tag="PW101_000926"
     CDS             complement(1359..1997)
                     /locus_tag="PW101_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2513345.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2001..2771)
                     /locus_tag="PW101_000927"
     CDS             complement(2001..2771)
                     /locus_tag="PW101_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDL2513346.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSTYWERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            complement(3189..3605)
                     /locus_tag="PW101_000928"
     CDS             complement(3189..3605)
                     /locus_tag="PW101_000928"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513347.1"
                     /translation="MMTREKEAYERFILLFVERFNQAFHTDYATNIFEDFENSYSYSV
                     ISSTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNGFSLHQVLWCLLGKKKI
                     IITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGI"
     gene            complement(3656..4037)
                     /locus_tag="PW101_000929"
                     /pseudo
     CDS             complement(3656..4037)
                     /locus_tag="PW101_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4163..4828
                     /locus_tag="PW101_000930"
     CDS             4163..4828
                     /locus_tag="PW101_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2513348.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5225..7258)
                     /gene="brxL"
                     /locus_tag="PW101_000931"
     CDS             complement(5225..7258)
                     /gene="brxL"
                     /locus_tag="PW101_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HDL2513349.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(7376..9898)
                     /gene="pglZ"
                     /locus_tag="PW101_000932"
     CDS             complement(7376..9898)
                     /gene="pglZ"
                     /locus_tag="PW101_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HDL2513350.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(10014..13646)
                     /gene="pglX"
                     /locus_tag="PW101_000933"
     CDS             complement(10014..13646)
                     /gene="pglX"
                     /locus_tag="PW101_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HDL2513351.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(13710..17381)
                     /gene="brxC"
                     /locus_tag="PW101_000934"
     CDS             complement(13710..17381)
                     /gene="brxC"
                     /locus_tag="PW101_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HDL2513352.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(17406..17981)
                     /locus_tag="PW101_000935"
     CDS             complement(17406..17981)
                     /locus_tag="PW101_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HDL2513353.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(17981..18580)
                     /locus_tag="PW101_000936"
     CDS             complement(17981..18580)
                     /locus_tag="PW101_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HDL2513354.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         5761 a         3564 c         2617 g         6744 t
ORIGIN      
        1 agttctaata aaaagattta gatgaacagt tttattgatt taaatagttc atatttttta
       61 tttataccgc ttatttacta cttgctgtac tagaatctga agtgaatgta ctcaaaacat
      121 tttcaaagtc tttgtctttg atttttacat tagcgtcttt caatacttcg ccaattactt
      181 tattctggaa ggtactgtca cttaattttg tgtctgttgc aatttctttc aattcttttt
      241 tgtatttatc catgtcgttg cctttgtttt ggtttttcac cattttaacc acataatatt
      301 ccgtagtata agttgaagca ttggtagaag tgatcacatc agaaacttga ccatctttca
      361 acttaaaggc tgcttctttg acttctgccg gaaccgttgt agtagtggaa tcaaatttca
      421 cttttccgcc atcttctttt gtcgtagtat cagttgactt gtctttggct aatttagaga
      481 aatcgtctcc cttatcagca gctttcttca catcttttgc atcatcttca cttgctactt
      541 ggataatttg agcttccact tctggatgga aagattccca agccgttttt aaatcttcat
      601 cggtcaattt gatatgtttc tttagacctt cttgaaaggc tagactttgt ttgatactgt
      661 ctttgaatgt ttgttctgtg taacctgcag atttcaattg tgaatcaaat gaatcaccca
      721 attgtttctt tgtttgatca tattgggaat cgatttcttt ggttgatacg tcatctccat
      781 atttatcttc aaatacttga taaacgatca actgttgcac agcggaatct gctccttgca
      841 tttttttcaa gctatccatt acttcttgtt cgctgattgt accgccttta taactaacgg
      901 ctgttttttc accggcattc gtacagcctg acaataagcc agtgcctagt acgatggcaa
      961 gtgctaaaaa tctttttttc atttatcaat tctcctactt ttcgtttttt gcgaattgct
     1021 aaaaacaaag gtgttcaaga tgaaaatcat caagaacacc tttactaatc tgctcgatac
     1081 gtcctgaata gatataaggc tacacaatcc agaaagttcg ctcagcagaa taaatgtcaa
     1141 gtagcacatc acaccttaat atactacttc ttttgttacc gtttttcaag tctatcttgc
     1201 gttccgtatt taacgtaact aattcttaga acaaatgtta tcgattttgt ttttcttctt
     1261 ccgctgttgg gatattcaaa tcagacactt ttatgttcga ttttccagac atttttttgt
     1321 tgtttttatt agaaaaagtt tgttttttca tttgttttct aaagcatgat cttcttgttt
     1381 cctattttgg tcagatatgg atcatgcgtg accacgataa ctaacgtacc ttgggaagcc
     1441 tgttgcgata agatttcaac gacttgctcc ttatttcgtt tatctaatga tccagttggt
     1501 tcatcggcta aaatgatttt gctattcttc aataagactc tcgcacaggc gattctttgc
     1561 ttctcaccac cagataatgt gtagataggc gtacttagct tcttgtttat atttactttt
     1621 ttcaatgcct catactttgc tgttcgctta tctgaaacat ttaaattcga aaacttcaat
     1681 ccaatatcta aattaaaatc gattgtttca tattcaatca acccaaaatc ttgaaaaaga
     1741 aaagagaggg catttctttt tatcaagcgc ttctctcttc cagttaaatg ttttgtttct
     1801 tgattaaata aataaaattg ccctgaactg aattgatcat tcaatcctaa tatattcaat
     1861 aaagtacttt tccctgctcc gctctctcca actacagtca caaaatcccc agcatccgca
     1921 gtgaaacgat ccaaatgaag aatcgttttg tttcgaatga tttttttcgc accttttatc
     1981 actatcaacg gctccaattg ttaacctcca ttcatcagca ataactgact tttcttttct
     2041 gtagacaaaa taaaaacaac tgaaagaata aaatcaatga agctaaagaa aaaaatatta
     2101 aaaagatatt taggctctct ttctataaga aaaaggaaga ttacacttgt cgttccccat
     2161 ttgatgaggt tcatcatgaa gaaatcttta tacgtttcaa agagcgaata accacttgaa
     2221 cgaaagatag cgattttccg acaattgtag acaaaatata tgcataacat gacatagctc
     2281 aacaagaaga aagaaaagaa agtgagtaaa aatatctgta tcaagtaatg aaatgatctg
     2341 cttaacactt tgacagctat ctcttcagtc tgagtatacg gaaatgataa ctgcaagtta
     2401 tcacgtaact gactttcttc aatcagctca aataaccttt ctttcgacaa agagcctatc
     2461 ttgatcttta acggtggata gatttctccg ctgagaatat ttctttccca atacgtactg
     2521 tttttcaatg tcagtatttc aacaaccgga taatgttcga tccaggggat agaaggaata
     2581 aatgtataaa tgggttgatt gttttttata aatagaaatt tcggttcttt ttcactgatt
     2641 tcttgataat attctgttat ttgcgccagg tcatttttta aatctgtatg tctatatcgt
     2701 tcagggatca atataacagg gtgcgtttct ttttcggtta tttggatcaa ccttccagag
     2761 acatcaatca aaggaaattt cttaagataa ttcagattta tttttatccc tctgccaaac
     2821 tgttcatttt cttcattcaa gtaatctgat acattgacta atatacttcc gtttttattc
     2881 aaagtttcat ataattcctg atcggctgct gatgcaaaac gattggaata aataaaatcc
     2941 acgtggtttt tcccgatggt tactggataa aatacatgat agtcctcacc agcgatttct
     3001 tttggtgttt catgggaaac agctaactgt gaagaagaaa aaacgatagt tgctaattcg
     3061 acattggcaa cggtcaaaag aagagaaaac aacaatccaa atgtaaaaag aatgtattgg
     3121 atatagttga acgcttctct taacgaagcg attttttcaa ctaataaaag agaaatcaaa
     3181 gagataccct atattccgct gaacaagaaa acgtacatct ggaacaaata aagaaattgc
     3241 tccttataat acataaaaat aaaaccacca atgattcctg caattatgaa tgtaataatg
     3301 attttctttt tccccaataa acaccacaat acttgatgta atgaaaagcc attattcaac
     3361 aataaactga tttctttatt catcaataag aaaaaggatg ataacaaaag gaataaaaga
     3421 atcaatccaa tagttaaata cactcgataa ttatatacgt ctgtacttga aataaccgaa
     3481 tacgaatagc tattttcaaa gtcttcaaaa atattagtgg cataatcagt gtgaaatgct
     3541 tgattgaatc gttccacaaa tagtaaaatg aaccgttcat aggcttcttt ttctctcgtc
     3601 atcaagaaaa attcaccttc aaaatctttt attttgatcg attcttcaat ggacgttacg
     3661 cgaaaggagg tattagaata gggtaccttc tcgtgagaag atttgacatc cccttgataa
     3721 tacaatgtca tttcaagctt tggctgtatc ttgaagttcc aagatgtatc tacttcattg
     3781 gattcattta ctgttttttt catataagaa agctcagttt ctgatgagac attttcaaat
     3841 accatgatta gctgctcata ttcttcttgc ccaataaaat gttgtggcaa tcgaatcgga
     3901 gtataagtat ctgtttgatg gaaaaattca tttgtccgat agagtacctg tttatttata
     3961 ataaccaagg caaaaccact catgataccg gtcactatga ttgtgagtaa aactgtgaac
     4021 ttttttttga ttatcaccac tctcccttac ttaaatgatt ctttgataaa atcataccgt
     4081 ttttcatcaa aaaaacgtat ctatcaaatg tataggtcat gtagaataaa tctatatact
     4141 gtagaatcga aaggagaaga agatgaacat cggagaaaaa atacgaactt accgcagaaa
     4201 ttgtaagctt acgcaaaaag aattaggcga aaaaatcggt gtttccgata aaacaatctc
     4261 tagttgggaa aattctcgta caatgccaga ccttgaaatg ctgtcacttt tacatcagac
     4321 tttaggtctg ccgattgatt ttccttctat tgaatcagct accgaatcaa tagaaccaac
     4381 attgccggat gaacctcttg ttatcaagaa atcttattca tcaaaacaac ccttctcact
     4441 caaaaagaca ctatctttct ttctgctcgt cttttgtttg gtctatcttt ttcttatcaa
     4501 ttatcaatat aaaaatgaaa cggttgatcg attcaatcct tttttgaaac cacaaatcgg
     4561 ctataccaca ctccctgtag gatacgtgga aaattccatc gaacattggg tatgtaatga
     4621 tatctttgtt catggtacat ggctgtcttt tcacgctgga gaggtagaaa aaggtaagaa
     4681 tcatgctgta atcaaacaca aaggaaaaca cgtcatgaac gttcgcctgc tcacttggga
     4741 agccattccc tcagattacc aagaaatcct catccaagac tatgacgatg ccttaaaaag
     4801 aagccctcag gagccagata ggcattaagt aggagatgaa cgtagagata gaaaaaatca
     4861 atgcacatga tcttatcaaa aaaaaacagg ggtacaaatt aattgaaaaa gctttgagat
     4921 gaaagttcct aaataaaaaa gttcagaatg ataggtaaaa aaatgactaa ctattctagt
     4981 attaggctat ttgtattatt cttctgttat ttttatatca aaaaatccag aaataactaa
     5041 atataggtat ttctggctta tagtataata tttacgccac agcccccaat gattcttttt
     5101 ggaatcatga tcaaggacgg acatacacaa aaatattttg cccattcgat ccgaatagat
     5161 taacgactaa tctagtaagt aatatagatt ggtacgtagt caaaccagca aatggaaaag
     5221 ttgtctattc caccccaagg gctttaaaga cagcatcttc cggcgtttga taaaaaatca
     5281 aattaaaggt acccattaat tctgggggta cagttccaat atcagcagcg cttatcatag
     5341 gtacaagtac ttttttcgct ccggcatctt ttgctacttg taaacagtta gctaaattat
     5401 ctaccttaat catagaacca ccaattgtaa tatcccctaa aataactaaa cttggtaata
     5461 caggtttatt taaggaaact gagcataaag ctattatagt tgataacgat aatgtttttg
     5521 tcataccaat gccattcata tcttgaacat gaacgacaaa atcttttgtc gtcaaactaa
     5581 tagactgact aacacttcca taattagctc ttaaatatcg gtatgccgaa tccatcgctt
     5641 ctcttgctcc agcatctgaa ttgatcccag tacgttcaaa ctttccattt cccgaagtca
     5701 tttgcgtttc caatttatag gtacctaatt gcccagtttt gccataagat accgtgtaca
     5761 aatgccctgg cttccctaac ccttctggaa tcaaagttcc tccaccttgt tcaggaacag
     5821 aaacatactc ttctcgctct tcttctaaat caatgtatga aaaattgaca tcataaaatt
     5881 ccattccacc aattttcttt aattgttctt ttacacgacg acgcatttca agagcaaatc
     5941 ttagtatttc ttcgacatca cttttttcaa atctaccatc aggatacaac agtttcatca
     6001 tcccagatac aatccgacga actgcaatcg tatcccgctg atttaattga gaaccaagtt
     6061 tgaaatattt atcaaaacta tcgccaaaac taacttttcg aagctcacgc atgacttcag
     6121 aaaaataatc tgtaatgaag ccatagtcat ttgtaaaaaa atctggtcga tatttaggaa
     6181 tttcccaacc tggtaaataa caatgcatcc gatccaaaaa ggctgaatcc gtccccataa
     6241 cctccggaaa tggctcaaat aaattcgaag ttttcaatat tacatcaacg ctttgattaa
     6301 tatttcctac aaaagccatt gatgctgaag cattcttttc ttctttgcct ctagcaaaag
     6361 atcctgaagc catgtaatct ttcatgattt gaacgccatc attatcctta aatttaatcc
     6421 cagcaacttc gtcaaaagca acacagtccc acatacctac aagtcccatt gtacgactag
     6481 ccatattata gaaaaggttt gctactgttg tttgtccacc tgaaacaagg attgagtttg
     6541 gtgaaatttc tttatataca tgcgatttac ctgtaccacg tggtcctagt tcacagagat
     6601 taaaattatt ttcaattaat ggaataagtc ttgctaaatg aagccatttt acccttttat
     6661 taaattgtgt aggttccata ccaatggacc gtagaagtaa gtcaatccat tcatttgtag
     6721 taaactcctt gcgagccatc ttgtaagcct caatgtctaa accaggcatt tggattggat
     6781 caaccttttt tgcaataaaa gggctattcc aacgatcatt ctcatcatac caatattcca
     6841 tcgaaacaat acaccagatc cccccaccta ataatcgatc aaattttttc ggatactctt
     6901 cactaatttg aacatttttt aatcccaggt tagagaattc tgcttcataa caatctttct
     6961 tttcatttaa atgtacagtt actttatcaa taactgtata accacccaat tcacgaattt
     7021 ttgcttttac tttttctgct tcatcaggac gcacatagtt tcgagcaaga atatttttaa
     7081 cattttccac acctttttca accaatgttt catccaatga cgaagcatac ataccaagta
     7141 aatattccaa tacataaaca ggtacattcg caccttcttt aattttcttt gttaaatctt
     7201 ttctgacaat ctttccggga aagaattcag ataactttaa aatttcctgt tgttccacta
     7261 gaattcctcc atgtgttcag tcattataat agcaattaaa gtatatcata cttaaccagt
     7321 aggcttgata atacaaaaga aaaagtatcc acaaaaaatt gtggatacct cgtttttata
     7381 tatcaaaatc aaatgattct gcaaaaggat tatctataat gaattttatt cgctcggtca
     7441 aatcattttt ttgatttttt atcactaaat aataagtatc ttcaactgta tatcgacgat
     7501 ttacaaattc aaaaacaaat ttagtaaacc gctgatttgc atcttctgtt tttgaatccg
     7561 caacatagat ttcttcattt gagatacgtt caccatattc atcttcaaaa taaagccaga
     7621 aagatgcggg gtcataaaca tcactaattg catttttttg atagaattct aacgtggttg
     7681 ataatcctgt aattctgcgg ttgctagtca ttaattcaat tgctactggt tcctttgcat
     7741 tgctaccttt ggctaccgta atttctagaa cagggactag catttcttga atagatgacc
     7801 ctccatgaac gtagctttgt cccccgccag ctttcttaaa aatacttgaa gtaatcggga
     7861 cacaaatgtt tctagtgtca tcatttccta aagaatctcc caaagagtaa tttttcgtac
     7921 caattatttc gtattcttct tcactaagaa taaaacgacg ttctacacga tctttttcat
     7981 ttctagatgg attttcaatc ttatctactt cttcaacgtg gcttctttta taaataaatc
     8041 catgatcagc cgtaactata aaacgataga tactgccatc attatgcaag cgattaataa
     8101 gtcgggaaat ttcgcttatc gctaaatcac aggcctcaaa aactcgattt tcattcgagt
     8161 gatcacctgt tctatccact tgatcgtgat aaatataaat gactgatttg ccattaaata
     8221 cttctcgtac ctcagcctta ttcatcttaa ccacatcatc ataatttaaa gctacagcat
     8281 ttgcatctcg ttgctggaga atcgcatttc gattggataa accattcgtt tttttcccat
     8341 caatcagtac ttctgttcct tcattgtaag ttagttgatt gtttggtaat aaggcggctt
     8401 taccgaattc agtaactgaa ggtaatacag aaagaaagct agccatgttt gttgaatact
     8461 tcttttctct aactaactta cgttctaaat tcatccctaa ctcatatcga aaagcatctg
     8521 aaataataac tacagtttta acagagcgtt tcattacagc ttgattataa aattttcgaa
     8581 tggatggacg attcttaaat tcaaaagttt gattccaaat ttgtcctgat tgatcaagaa
     8641 acttcgaata atttgcttca actaatcgat acaattggcc atatttttgc ttttcttcta
     8701 tatgatcact attccaaata aatttccgat aatgcgtatc aacccaatac atttccttct
     8761 catattgagt tagcaaatta tccatgttca tcggcaagca caatctgttt ttatccacaa
     8821 gataataagc atgtttcaat aattgatact cataaatata atccgatgcg aaatgcattt
     8881 tttcgcgctg ctttaaacta tcaaaaattg tttgtcctgc aattttcgtt gtagtgtcag
     8941 aagcctttaa tcgttctgca taaaacatta atatttttgc atgaatctca gaaaatacgt
     9001 ctgcttcaat tagatcatca atttctatac tgtctaataa tccatcacca tcaatagcct
     9061 gatatacttt tttagatagc tgttcaaagc tttccatgta acgtgtatca ttcataaact
     9121 gatccatgaa agtaataatg tttgctttca aatgtagtat ttcaaatcct ttgaacttct
     9181 tcggcatctc ttgatcaatt tgataataaa aatagttcaa ataaagacac atcgtcagct
     9241 tttccaatgt tggctggcta tccgtaaagc caaatttcat ttgaataaat tcccaaaatt
     9301 tagctgttaa ctgatactta tcaagttccc ttaaaggatt ctccctactt tctgtaaagc
     9361 tttttagaac tacacgcagt aaatcattta atcgaatact tttcgatttc aacaaaacag
     9421 ccattatcat taattcaatc gtttctgatt tattaattgt catatgcgta ctaaattcac
     9481 caaaacttct catgcgatcc tttgcattaa agaaattacg atactgttta actgtctgat
     9541 gaaatctaag tggaatagcc aattcattca tgataatcgc taagcggtct gcactaaatt
     9601 cctctgaata attcaataca ctcaacaaat gattatcttc atctttatca tcccaacgag
     9661 gaaatggcgc atagattaaa tagttagttt cgggatcttc atattccaat aaacgcttag
     9721 ttttaaattg tgcgtccttt tctaatcgat agatttttgc attttgaatg accaatgagt
     9781 caatttcttc aagaaaatcc tgatttgaat caaaccaaaa aatgattttc ctacatccat
     9841 aattaggaaa acgttgattt aatttttctt caatttgttg cgtatttata tctgtcatca
     9901 atctcaccta atcgttctct ttctataccc cttatattga cataatttgg ataatttcgc
     9961 acgcaattta agaataaaaa agaaagttgt tagaaaacaa ctttcttaga taactatttt
    10021 tttggttctt tgaccccttt tgcaaaaatt tggaatttct cgccggtttt aggatcacgc
    10081 tgcaacttat cataattgac aattacacca tcatctaagt ctaattcaat tcgttttaat
    10141 gctaagtgac ccaacttctc gtcatactca cgaacttctt ttaattgctt ctgaatcttt
    10201 acaatttctt ttgtcattgc atttctttct tttgggttat cacttgttga aatttgttgc
    10261 tgacgcaatt cgattcgatt gtcatacgct ttttgtagtt cgtgtaggta ttcagtacgt
    10321 acttttccta attgatctgg tgtatagcga tgcaagtaca ttaacgcttt aaagccattt
    10381 tgttttcctg agtccaattg ccaataaatt ggtcgattgg agtatgtttt taaatggtct
    10441 ttgtaaaaat cttgtacgaa gtaacgacga ataatttctc gacttgtccc tttgccacct
    10501 aaagtgtcag cgataaactg gagattttct tcaagggttt ctttaccaaa agtaatctca
    10561 acgaaatcaa tgaaacgatt gacaatgtca atttggctat ccttgaaata ctcatcatcg
    10621 gtgatgagta aaacattatc agtatcaggt ttaaattttt gatagcggga aggatcccat
    10681 tcgccgccag cgaagatgag accatcttcg tctagggagt aacgaccgaa gatacaacct
    10741 attgaataag aaataaaatc cttaatttcc ctcgccttat caggtaacct tgcagagata
    10801 tattcatctt gaacctcttt tgttaattct ttttcaagag aataatgatt aatgaatatg
    10861 tcgttcagtt cctcttcatt cgattttaga gtattaaatt gattaaccaa ctgcctactt
    10921 agacagttaa atgcctctga gagatctttt ttaaacatat aacttaacaa aatgtttctt
    10981 ctaaactcgt atgacgtttc aagagcatta tagtcttttc tagctatatc gatattttca
    11041 tccacttttt ctcttatttt ttttaatttt ctctcagatg taactttaag aaaaggtagt
    11101 tttcccattg gtccttcatg gtaatccaag gtgggagaaa taaaagttaa aaagtgatta
    11161 actaatttag agtttacata gccaaatatt tcttcaaagt cagttatatc ctttgagaac
    11221 agaacagatc ctttagtttc aaaaattgcc tgattctgca atctaaaaga tacttgtcca
    11281 gatgaaagtg tcgaccaagt aattcccttt ttaaaatagt aattaagatt ttgtggtcta
    11341 gattttaact tcccattttc attaacataa ttttttattt catctccatc attctcccag
    11401 ttaaccacgt attcattgtt tccataccat tttctaaaac gaccaccttt agttgtagga
    11461 aaccattttt ttctagactt tttagcgtcc tctaatgttt tagcatcaaa ctttattttg
    11521 ttaaattcta cttcaaacca ataccgcaaa aatttattgt tatttccggt tgccaaccct
    11581 tgttttgcat ctgcaacatc gttagttaat tttccttgag caaaatcagt gattacgtta
    11641 tcgcttgccc agtaagcaat cggcattcct gggatttttt tgaagttgtc ttgattagta
    11701 ctataaaaat aatcgcattc accatgctga tgaattgctt ctaacgtttt aattttttga
    11761 acttccattc cacctttaaa atctgataat tttatatagc ttccatcttg ttcatttgaa
    11821 ttttttatcg taaacgtaca aattggaacg gttgcttctt caaaagctga atactccatt
    11881 tgaatcaaac tagaaatgtt tctttcatta attaaaaatg agcgtaactt ttcatagcta
    11941 gaaatgaaca tccaaacaaa tggcgtcata aaggaagcat atccattatc aatcagcatt
    12001 tggctattca atttaataaa cacagaaaat agatcttttt tgacatcaga atagttatca
    12061 tttacatatt tttttaattt cttatccatt ttattaagat aaggcggatt agtgactacc
    12121 gctacatatt tatcaactag catttcagcc acgtcaagaa tgtgcaagta tttttcttgt
    12181 atttccttca tcccataaag aatatccata ttcgtaaatt cgttgaattt tgatatatac
    12241 tctcttactt ttcctaagta ttctttagtt attgcctgat tatttttatc gactaactta
    12301 atgattgatc caagttcttt cccattttca aattgatcaa aaattaactc tttgtattca
    12361 ttccagcgtt cactatactt atcattgttc aattcactaa tacgctcttg atactcgtct
    12421 gtcactttag gagaatcaat aaactctctt aaattcggcg atagactttt tttcaaaaaa
    12481 agacgatcgt attgacgccc tttcatcata attgcaaaat aagacaattg atacgctctt
    12541 ttatcgattt caagaccata taaattgtat tcaacgattg cacgtgcagc atcacgctca
    12601 gaatatccta acgtaagata aatttccatc aatacatcga aggcgtaaac taaaatatgc
    12661 ccagacccca ttgcgttatc gataattttt agttcttctg gttttaaatt acttacagat
    12721 gttgcttgtt caaaagcata tttatctgga attaagtatt tcaacttatt tcttaattca
    12781 ctattgggat aatgatctaa ccaatacttt cctaaactat tgtcaaccat ataacgaacg
    12841 acccaatctg tggtaaatag ttgagttgct gccggaatat cttgtttttt gacaatccct
    12901 ttataaatat tcaccacttg attatgtggc tcactattat agtactgata caaccaccca
    12961 ataatctcta cctgtccacc agcttgaaca tagaaatcat cctctggaat acttaaaaga
    13021 tcagcgatta caccatcttt tttaatataa gaagctgtaa acaaaagttc tgtataatct
    13081 tctacttctt caaatagctc aggtaagtaa tcattcaatt gattacagat ttttataaaa
    13141 atcaattgat acagcttgtc cattgcttct gctgaattat ctgttttcca agttctgact
    13201 tgttgctgtt cattactaga taattcttca aagatatcgg catcataaat atctgtaata
    13261 atatctggct caattttccc acttatttca ctagataata cacgatacgt gtcaggtaaa
    13321 taatcattta cctccataaa ccgaatggca atcaatcggt taaaccacgt ataagcaacc
    13381 tcttcgatta actcttgaaa agcttctgaa taagaagtga cagaagcacg ttgttttagt
    13441 tctgcaatca atttgtttct ttgcgctatt tcttttcctt gaattcttat tcctgatttt
    13501 tcgatttctt gtaaattgct atcaattttc ttcacatctt caatttttga ttcatcaatt
    13561 cctaaacgtt ttgctttagt cattacttca tcaattaatt ttctacgggc attgaccgcg
    13621 aaattcttta tcgctttctt atccattttt ctttcccttc tatatgtaac ctttgaaaag
    13681 acaggcacct ttttatgcct gtcttttcac tagaattgaa tcgtgatttc tgtattttct
    13741 tgaagttgtt ccttcagttt ttgattaatt tcagctgtta actcatcaat atctgctgct
    13801 gacttaatag ttttaacatg aacgttaagt gttctcattg gtactgtttt ctgttgaata
    13861 gtaggtttta ctatcgctat ttcttcagct tttgctgcag agtctatttc atctttttca
    13921 gtttcacgaa tcaagtttgc tgcttcaatt tgtttacgtg cttgttcttc ttgacgtctg
    13981 cggttttctt cattatcgaa acgttgatta aagatcaaat actgttctgc tacgtttaat
    14041 gataagaaaa caagatcatc tatagtattc gcttgttcaa tttttgcttt cagttcttga
    14101 aattcttggt caactattgg tacaaattga tccttaaaat cttttccttc taataattta
    14161 aatgttgctt gtttttcttc cgttaattta tttaatattt cctgactttc caactctaac
    14221 agttgattat attgttcaat aaattcttga atcaactcat ttagtttcat catatctgaa
    14281 taaggatcag cctttttcaa aatggttttc atctcatgaa tcaattgatc aatttcttgg
    14341 ctagaagttt gacgccgact tttttcgatt aatctaattt tatcttgtgc attctcccag
    14401 atttttttct gagtactatt ttgcttgtgg aaatttttaa ttttatcata tgtttcagcc
    14461 cagtcgaata attcatcgcg atattttttg attgtataaa aaaagtcttc aacatctgac
    14521 gtttgaacaa ttacttcaag atattttttt atttgctcat attcataagc actagggtag
    14581 gtataagtgt tttgtaaatc aaaataagag cgtaaattgt ctaattgacc ttttaaatat
    14641 ttcttgaaat tttccattgt atgttcattg ttattttcat caacaatatt acgactttga
    14701 aacagttctc taccgatttc gtcaaccaat ttttttgctt ttggcgaaat caccgcgctt
    14761 ggttcaattt ttatcttttc actttctcgt cgtttttgaa taatcattac aatactattt
    14821 ggatcatcca tccgtgaaat ttgtacattg ttataaagta aatcaatttt accagacatg
    14881 aaaagtttag cgatgcacca ttccacatca agttctgtga atccatacgg ttgttttgtt
    14941 tcaaaacgat cacgaattgt tttcatcgta atcgttaggt gatttctctt ttgatcattg
    15001 ataaaattga aaatttcctg aactgcttgc tcatttgatg gatcttcaat aatttcattt
    15061 ttgctttctt tcactaaata taagatatct tctttttctt taggcgcatc aatttggttt
    15121 aatttatggt atacttcttc agccattttc tgaagtgctg attggaattt tgctttaaag
    15181 tcggagcctg tcagttgaat ttctgcaccg ttatagaaaa attttgcaga tttcaacagt
    15241 tcatttaatt gatcgttgat acttgcactt agttcctgta attcaccaga tttttccgaa
    15301 cgaatcgcgt caaagttctt tactacactc gaataagtcg ttgttaaata aagattcatt
    15361 ctagcataca ttgctatatc atctttaaat tctttatctg atggcaattc tacgaataat
    15421 aagttacggg aactcttaag cgcaagttct gcctcttgac ctgctaaatc agagtatggt
    15481 gaaataaaat taactgtcat ttcagcattt ccagcttgct tgtatggacg ttgatctaac
    15541 acttgtgcat aagtaaacgt ataccggttt ttaaatttat ttccacctga catgcctaat
    15601 ttttcatagg caaaactatt tcggtctaga acgccgtcaa aaattcgttt agatagttct
    15661 acgatcaaat cactatctgt aatattttga tttttaatct cacgagtaat ttcctgttct
    15721 tcatcagttt ggaaaatata actatctaag ttcttccgca caaagttttc cttaattaaa
    15781 aggtccaatg cttcttctac tcgtttttta gtttccaggc ggtcttcgtt gatatttctt
    15841 atcatcaagc tcgtaatatt ttcaacattt ggtttgatgg cttgatcaac atatttaatc
    15901 atgaataaaa ttcgtaagac atttacgtta aagttatcta tatcagttgg tctttgaata
    15961 tgagaattat ttgtagcttg tgtaatcact cggctaattg ctgtatcaat ccaacgttct
    16021 aacgagtaat aaaactgatc taaagaaacc aaactggttg tatcattttc cttatttcgt
    16081 tcagcggctt ctttaaacat tgctagtaat gaacgttcac cagacgataa gtttgatcct
    16141 tgatagctgt gattacgaat ctcggttaga atttgcccca ataaattgaa ttgataggga
    16201 ataaatggat aaacttctgc gtaattttca gcatcttgga agagctttaa ttcaggacta
    16261 tcaacaaaga gtaatttatt tttcaattct gtttgaacag tttcaaattg taaggctaaa
    16321 gaatcattgg caaactcttt tttcttcaaa atacgttctt taattacttc atcggcattt
    16381 gctgaagata aactcacagt tgtggcaaaa cgatccttga tcttagaaaa atcattatca
    16441 gaattgttat caaagtttgt tacgtgatca atcgcttcct gacttgtcac taatacccaa
    16501 gccttacctt cacaaattcg acccaaatct tccacaacgg tttgtaactg caaagtcaac
    16561 tcacgattac cgccgatata ttgtccgacc tcatcgacta gaaaggcaat atgatggtct
    16621 gcttctttgg aatcaagata gcttttaact agatttgcaa agtcatcaat gcttattaaa
    16681 taatttcctg atgctttttc taaaaaatta tcaatagcgg cttcactcat aaaatcgatt
    16741 ttaagtgccg cttctttgat atcatcttgg atccaatcaa actcatggcg ctcttcttgc
    16801 cattttgccc cattaatctc ttcaaaagca gctttgaatg catcaaactt ttcttcttta
    16861 attaaccgac gttcaaaatc tgctagatgt ggaatcgtgc ttaatccttg catttcgtta
    16921 aataccttta aaaaaacatt cacgattccg tttttgtctt ttttaccacc tgcatcactt
    16981 tttgaatcaa tattaaacag aataacatct gtatttattt gtgagcaccg ttccatcgtt
    17041 gccaaagtca gtggatcttt gattttccca tcttctttga agtaatcaat ggctttcttg
    17101 tcatttattt ctcgatttgc gagcaagtaa gaaataattt ttaagaaatg agatttacct
    17161 gaaccaaaga acccctgaat ccaaacaccc attttatctg ttggtgtatt gatactccgt
    17221 tggtaggcat caaaaattgt tttaaagtgt ttttgtagtt ctttcgtaac cacgtattct
    17281 tctaattctt gttgaacatt ttcatccttt gcttgaccga ccttaataac tccttgaata
    17341 tttcggtcaa ttggttttgc aaaaatatct ttaattagca tttaatctcc taccttccat
    17401 cccaactaaa ttctaaatgc tcgataataa ttatcatctt tattggtatt aagaattgtc
    17461 aaacttaatt catcatattc ccctggatac atcatcacaa ctggtacgcg atcaaatacc
    17521 tgatgcattt tatttaaaat agagtgcgct ctaattaatg gatataattc gccaattcct
    17581 gtcaggaaca caactgattt ttctggtgta tgggcatcca catattcaaa aaatacattt
    17641 tcttcctcag gtaaatccat tttcaataaa cgaccaatgc tttccactaa tttttgtaaa
    17701 ccacgtcgtt cttccatctt aatcgctttt tcagtgaaac cgcgtttttc tagatactct
    17761 aacataatca catagagatt ataaactttc acttcataat tatcaccaac agatgttcca
    17821 gatatatcat taatataatc tcttacaatt aactcgtctt tcggatcgta gtcaaagata
    17881 taatagccta cttcattacc taaaccacga ttcaataaaa aatcttcttt ttgaattact
    17941 tcattcaact tttccaaatg ctcttgaatc gataatgaca ttataaaatt cctcccagtg
    18001 ctagtacaat atctttacga ttcttgtcaa ctaaccattg aatcaatcgt ttatctaata
    18061 atatcggagt cattcgtcca tattcttttt cccctctgac taatcctgtt tcaattagat
    18121 tcgtttgata agcggcaata agccgtttaa tcgtataatc tttccatttt gaaatatctt
    18181 ctgaacttct ccgcttctct gtaaaaaaag aacggtaatc aactggtgtt aattgcaaat
    18241 ttcctttacg tagattttca gcatatacct cagtcataaa ttcattaaaa agatcatcaa
    18301 tcataaggat ggaaatcaaa ttcactaact tttgattatc tatatttaac tgattgaaaa
    18361 gttgcaatcc agcttcatcc agttgactaa ctctcctttt cacgttctga tagatttctt
    18421 tttgacggtt actgctcact gcaaaaaaaa tattttcatg ttcatttttc tctttaattt
    18481 ctatatctgt atatccttct agtcgtaatt gaatatataa cttgaactct gaaaaccaaa
    18541 agcgtttaga aactagacca gcagaatatt ttttattcaa tagattgcct cctaatcatc
    18601 tataattcaa taagtaaaca agtgtttttt gctcatcgtt agcattcatt ttttagtatc
    18661 tactatacaa tacatattta ttatag
//
LOCUS       DANKHL010000039        18293 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000039 DANKHL010000000
VERSION     DANKHL010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.041"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(369..995)
                     /locus_tag="PW101_000937"
     CDS             complement(369..995)
                     /locus_tag="PW101_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513355.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1290..1625)
                     /locus_tag="PW101_000938"
     CDS             complement(1290..1625)
                     /locus_tag="PW101_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL2513356.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1612..1896)
                     /locus_tag="PW101_000939"
     CDS             complement(1612..1896)
                     /locus_tag="PW101_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL2513357.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1952..3475)
                     /locus_tag="PW101_000940"
     CDS             complement(1952..3475)
                     /locus_tag="PW101_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2513358.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3468..4643)
                     /locus_tag="PW101_000941"
     CDS             complement(3468..4643)
                     /locus_tag="PW101_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2513359.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4647..4832)
                     /locus_tag="PW101_000942"
     CDS             complement(4647..4832)
                     /locus_tag="PW101_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513360.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4798..6492)
                     /locus_tag="PW101_000943"
     CDS             complement(4798..6492)
                     /locus_tag="PW101_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2513361.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6489..6962)
                     /locus_tag="PW101_000944"
     CDS             complement(6489..6962)
                     /locus_tag="PW101_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDL2513362.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(7031..7186)
                     /locus_tag="PW101_000945"
     CDS             complement(7031..7186)
                     /locus_tag="PW101_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HDL2513363.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7320..7700)
                     /locus_tag="PW101_000946"
     CDS             complement(7320..7700)
                     /locus_tag="PW101_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2513364.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7704..7919)
                     /locus_tag="PW101_000947"
     CDS             complement(7704..7919)
                     /locus_tag="PW101_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513365.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7922..8329)
                     /locus_tag="PW101_000948"
     CDS             complement(7922..8329)
                     /locus_tag="PW101_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HDL2513366.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8595..10070)
                     /locus_tag="PW101_000949"
     CDS             complement(8595..10070)
                     /locus_tag="PW101_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDL2513367.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(10060..10908)
                     /locus_tag="PW101_000950"
     CDS             complement(10060..10908)
                     /locus_tag="PW101_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HDL2513368.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10945..11307)
                     /locus_tag="PW101_000951"
     CDS             complement(10945..11307)
                     /locus_tag="PW101_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513369.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11308..11466)
                     /locus_tag="PW101_000952"
     CDS             complement(11308..11466)
                     /locus_tag="PW101_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513370.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(11607..12231)
                     /locus_tag="PW101_000953"
                     /pseudo
     CDS             complement(11607..12231)
                     /locus_tag="PW101_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12228..12539)
                     /locus_tag="PW101_000954"
     CDS             complement(12228..12539)
                     /locus_tag="PW101_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513371.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12583..12876)
                     /locus_tag="PW101_000955"
     CDS             complement(12583..12876)
                     /locus_tag="PW101_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513372.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            13070..13717
                     /locus_tag="PW101_000956"
     CDS             13070..13717
                     /locus_tag="PW101_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2513373.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13778..14923
                     /locus_tag="PW101_000957"
     CDS             13778..14923
                     /locus_tag="PW101_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2513374.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(15362..16180)
                     /locus_tag="PW101_000958"
     CDS             complement(15362..16180)
                     /locus_tag="PW101_000958"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513375.1"
                     /translation="MVQGLFGGGTSYEGQSISDIKEDVNNWKIYTENVKSQIVEKKEA
                     LIKNGFWNKIPWNFQQTINESIFCLGTFLSDFQIVESAFEENFISEREIKLLRKIGKN
                     SVQFNHSYGKYFKEEYEWREYGNPEFEIVESIYQHGRDFFVTLQDASNAAGRLEDYMS
                     NNSIYNKVNIFGNSYNTNIQQGHNNNMIVSIDDMSELLSKLDLLLENLNDSLPEEEVE
                     EAQEYIETIKDEAIKEKPKKTMLRFAVDSLKKLKWSTEFLASVATIAQLVAPLL"
     gene            complement(16280..17605)
                     /locus_tag="PW101_000959"
     CDS             complement(16280..17605)
                     /locus_tag="PW101_000959"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513376.1"
                     /translation="MENIEETFLKVLSPYISDSILRFQEQEKNYKFGFDIKNYNDSLH
                     VVFQYNKTGKIGIKEVHIFQIFLAKVNIEASKSKLNYTTLLDTNALFFSKKLLPYLHS
                     YEWALRKLIYLLAPAYFSDNWAEESIPNEIISSIKRKQIQAIGKFDSKNLLQWIDLYD
                     FEEYLFGENYILVTQENEENIVRYKEISQDQLLKLFQDNSHTISDSYSLWSEVFSKYM
                     DVKLEEIQTDMSLIREGRNIVSHNKEIEMALYQKLIKKLKRYTNQLEKAFQKILAGDI
                     AGEDLSDMSDDFEGYIGNYFSNIDLAGVTKLSESLSAMKVLNTDSMKKSMESIGQTLS
                     VQQEIMQNFDTEGIRRATEGINQSLQSIKILNTDSMKRSIESIGQTLSALSAQRAIMQ
                     NFDTKGIRSATEGINQSLQSMKIVNTDSMKRSMESIGKISSTNQVLKSE"
     gene            complement(17774..>18293)
                     /locus_tag="PW101_000960"
     CDS             complement(17774..>18293)
                     /locus_tag="PW101_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513377.1"
                     /translation="FSYRFLLHMRNYSQHGHLPVNIHQQRVYFDLDEILAMPHFDFNG
                     QLKKELDVLRKDIYNQFRDSPRISYVYTVAKFNLVITEIYFNYLKEIKPILIKLNDEK
                     NELLLNTKFQLINSDGSSSDMLFYEFDGENYHCFNRKDNSISMYADIKREVKKILKVE
                     EQYYKEIEKRNQ"
BASE COUNT         5335 a         3678 c         2796 g         6484 t
ORIGIN      
        1 ggaagagact gtgaagtcaa cgttttgacc tgattattag agaaacaaat ggcaaaatag
       61 ttcttcatat ttcgccgttt ttcgagcgaa tgccgagggg cagctgattg aacatcgttt
      121 atttggaaaa agggattgtg acaagacgtt tgtcacaatc ccttttttta tatcttatta
      181 attcattctt ttgttctatt cttcttcttc cgccatgaat gagaaaccca tatcaaagaa
      241 ctataaaggt tatcaaaaca gcgtttatga ctaaatgaat tagagttatc tatcactatt
      301 tttcaagcga tttaaaccaa atggcataca atatggcata caaaaacttc aataagccgt
      361 gggggttttt aaatttcttc ttgttcttta aaatcaatga gtggagctac ataatttgaa
      421 tccacttttt caaacgtcac tttttgcatc gacgaatgtt ttctttccca agataacgta
      481 aatagatttg actctggact tataaagtct acttcacctt ctagtattac tttcttatgc
      541 aaatattcct ctaataaata tagacttgct aaagcataca acacattttc caaatttgct
      601 tccttataat ttgaagaacg accatgtttt actctgttat atattttcca ccaatctggt
      661 gtatcttcag gccacccatc tccaaaaggt tttagtgaag tcaatgttaa agaattcata
      721 accctaacac cattatccaa acacattaat tcgctagatt taataacagc tttaaaatca
      781 gttatgcttt tattgctact gctatcaaat ccgcaaagtt ctttgaaaat aatctcacac
      841 tccgaaccaa tcgctagtag tagtttttga tattcaatag agaatgtttt gaaattattc
      901 tcatcgagca tcacaaaatc tgtggtatta ataaactcat tttctaaatg tagataatac
      961 ttccaataaa aatttgtaaa ctctgctttg tccattaaat cacctctttt gaattataac
     1021 aaaaaaaaca cgtccacaac agacgtgttc tttgatttct acacaatcag cttgccaaaa
     1081 ccatccaatc agtcctagca tgcctgtatt aaaattgaac gttcatcatc tatcatcttt
     1141 cttagtccag catgtttgca accacaccta caattttcag tccaacaata gatttcgact
     1201 agctaagtct tgcaaaattc aggcagctaa cccttcattt actaccttaa ttatactaaa
     1261 tacccacaac gtttacaagt gtaatcattc taagaaatct gtttagctat gatcgttgta
     1321 taatctttaa acgccgttcc ttcattgatt gaaataattt tataactctt gtttttccaa
     1381 gctactttca tatcgtttgt tagttcataa tcttgatcgt atcgaataat aaacgtcaac
     1441 gttccttcaa gaatggtacc tacactagca agcacgtctt ttagcatttg tgaacgcaca
     1501 ttagcccaac attcaaatac agtttcttca ctatccacca tctgcccatc ttcatctttt
     1561 acccgttttg tctttttaaa cttgactctc tcagtcaatt catttacatt cttaatgatt
     1621 ggcataataa gcacctctca tttgttgtac taatgcttgt accgttactg gtatccgttc
     1681 gctgctggct tcttgacggt tcaaatacca atgttgagta agtaatgaca ccgccaaatc
     1741 aaattgctga taattatcca tatctttatc cgttgcgcta ctatcaatag cactttttat
     1801 atattcgcct gctgtatcaa tcaattgttg aataaagtca tcatcaatag tgtgatcgat
     1861 ccgcatactt ttttttatgc tatccaaaat taccatttta tctctcctaa tgaaaaaaag
     1921 gcagtgggat aaaccgccgc ccaatatttt tctatacttt tggtgttaca actggcgtaa
     1981 actcaatata cactgaagca ttttgatcaa ttttcttata atcgttgcga acgatcactg
     2041 caagtccttg tgagtagtaa tcgaattttt cccattgagt cgttacttga tttctacgag
     2101 caacaaagat agattgtgca atatccccca taatcatagg gaatgttcca gctttaggat
     2161 tggcaaacaa tgtatctgca atcaatacta ctggcatacc gaataatgat ttaccactag
     2221 gtgctgtcac atcgggttgt aaaatgtaac gtccatctga atctttcaat gtatctaggt
     2281 ggtaatatcc gctttggttt agaattacca ttttatttaa tgctgggtct aatgccacat
     2341 tgtatagttg tttcaaatcg tccaacgtag cagccgtttt cttagtgaat gtttttaata
     2401 aatccatgat atgcttattg tcagtgtttt ctaccaattt cgctaactga tctttgacct
     2461 cttgtacaat attcactgct gaatcttcca caacctcatt tgataaggca atcttaccag
     2521 cacgagtttc tactttataa tcaactgaag taaacatttc agcgtcaata tcaccaattt
     2581 cagcaagttc agcttttgtt gctaacactg cttgttggtt agttgctact ggatatttac
     2641 cttgtccatt tgatactgtt tttactgttg catattgagc taagttataa tttgaacgtt
     2701 tcaaatcaaa gacttcaccg attacttctt cgggaacaac tgccgctgca tttacagtag
     2761 tgactccatc acgcacttcg ccttgtgaac ggatatattc ttcataccca cgcaattctg
     2821 ttttttcgtt atcgattaaa gttttttcca tgttattact ctccttttgt gctttagaag
     2881 tggattctaa gctattgttt gtttgttttg cttggttgtt actcataaac gattcgtaag
     2941 aacgcttatt tacttggaca tttgatgaat cataagcggg aacggtcacg acactaattt
     3001 cattaagtgc tttgatttta tctattgatc gttcaattgt gccatcttct tttttgtcga
     3061 atgaatctaa tccaagcacg aacccaaagg acatagaatc aacaacccct tttgagatat
     3121 tctcatacac atcatttgaa taggtcgtgt cggttagctc tgcttcaaaa tgtaatcctg
     3181 tatcatcgac attcaatttc aacgtacctg ctttgacact agctagtggc tttgaataat
     3241 cgtgtccata taataagaac acatttgata aatccacctc ttttaatgct tcgggtgtaa
     3301 tcacttctac aaagccgcct aaatcttttg acggttgccc gaatttcaac gcatagcctg
     3361 aaatagtttt gccgtctgtt ggttgttttt cattttcttt ctctactttg ggagaatcgg
     3421 ctgttagctc agcttcttct gtcagccgtt tttccttttc ttcttcatta ttcaactggt
     3481 gtaactcctt cctgttcttt taattgatat ttctccaacg tatctaaata cgtaaagttc
     3541 aaacttgcta gtaaacgatc gccaccatcc atggggggta gccccatttt cgaccgcccc
     3601 tcattaatcg tgagaagtga ccctttgacc tgatccaata cattttttac ttttgtttca
     3661 ggatcagtct ctagtaactg atcggaatta aagcggatat tggtttcaag tttcgtatct
     3721 aattcactca caaacacatt aaagtaatga atcagcgtac tttggatata ctggagatta
     3781 ctttgaacgg tgctagaatg ttcattttca acgcctaaac gctcaatcgg tacaccaaac
     3841 gccttggcaa tttgttttgt gttccaatca ttggaattga taagcttcaa aacatcggtg
     3901 tttatttcta atgttttgta gtccattgtt tcatcaagaa tgatcgttct aagagcatta
     3961 tctccactac ttgaaccgtt ggcttcttca aacttttcac gaattgcgtt ctttgctgat
     4021 ccgtctaaat ctgacttatg aaccttcaaa atacctgacc cactgacacc acgagtaaag
     4081 aaattgtgca atgtgcggtt gccagcttgt tgaattttca gttcatcacg taacgcataa
     4141 agtggtggta atcctgttaa accatcttgg gtgaaatatt tgaagtgcaa aatatcgcta
     4201 gacttcacac gtcttttttt atcaccaatc tgataagaca acgtaccatt atctaattga
     4261 gtaaccgtta cacttgagtt aggtaataag tgaatttctt ctaccttttc accgttgcgt
     4321 ttaatctctg caaagctatt accgtttagc aacatattga ccgccaaagc aaactttaaa
     4381 tgccaaccat ccatttctga attaggtttc tcgtttagta acttcaccag ttcattatca
     4441 gcttgtggca tattgttttt gaccaattga atcggacttg aagcaatatc actagcaata
     4501 attcgaacgg ctgcaaacac atcactattt cttaatgcac gaacgcttgt aaagctggtg
     4561 gtgtaatcat ccgattggat tgataccaca tgttgtagga aaggatctcc cgttgtattt
     4621 tgtctcgctt taaaaaatgc catcttttaa cctcccttct gttctgtatt tagcatgacc
     4681 gctaccccaa taagcactat gcccagcgct aacaagccat agtagacatt tgttagtaag
     4741 gtgatagcaa ccaacacaca caccaatcca agaagtaaaa gaacggtatg aatgtaatta
     4801 gaaagaaaat tcttcacttg tataaaattc attgtctgct ttcgctcctt ccgttgtggt
     4861 gaagtaatcc attgcaaaca cataggcatt gattaatgca gctattggat caattttgtt
     4921 actgttcttt gatttattaa tctgaatgcc gttgttatcc gtttttaaaa tagcgttgtt
     4981 cactgcatgg gtgagaatcg tatttttatt gtgaataacg ttgccttcat aaacttgttc
     5041 tctaaaagtt cgggtcggaa cgtttagagt aatcgttcct tgtcttactt ctaacattgg
     5101 gtagttggct ttttcagctt tactgataag cgaattagcg ttatacgggt cataacaaat
     5161 ccccatacat tccaaatcat tttcttgaat caaatcttga ataaactgaa atacttggtc
     5221 atagtccacg attccacttt ctaattgagt aatggaacac tcaccagctt tttctagttc
     5281 tcgataaggc aaaccgtcgc gcttttcttt gtcttgaagc ccatatttgg tggctacaaa
     5341 gctatgtgaa tcacaataaa gttcgccgtt atctagcggt acgatccatg aaacgctagt
     5401 taaatcatct gttttagata aatccactcc gatataaacg ggcttgcctg taatatcttg
     5461 tggttctact tcggttgcct gccagtcttc agcaatcata tagctgtcct cactcgcttg
     5521 tctccataaa ttgaagtttt tcaccaatac agaattaagg ttattttgtt tcaaagcaag
     5581 gttcacatca tcttgaatgg ttggaatcat tacttttttg atttcttcac tttcaaaaat
     5641 cggattggct ttgatccatt tctcttgatc gtgaatttct tcgggatcgt ccaattccca
     5701 tatcgctata aagtacctgt cggcttgttc ttcgcctttt agcacacgat ctaacaataa
     5761 gtattcctcg tacataggaa cgtttaaatc aagcccagcg gtactaataa cacacaataa
     5821 gccgttcttt tgttgggtca ttcctgactt aatgacgttg tacgttttac gagtttttgc
     5881 ttcgtgccac tcgtcaagaa ttgcggttgt tggtgcaaac ccatctagcg tgctggtttc
     5941 actagccagt gccatagcga acgaattaga gggcaagtgc tgaatcttag agttcataat
     6001 tttcagttgt ggtcttaaaa acttactaga cttgaccacg tttctaagtg aattagacag
     6061 catatcatag cccaatttag cttgtttcaa agcgttactt acaaacaata cttgccttgc
     6121 ttctgctggt tcttgttcca taatcaacgc attggcagcc atgccacttg cgatatacgt
     6181 tttcccattc ttacggctca tactggtaaa cgctcgattg aatcgcctgt aattcccttc
     6241 tttagtacgc cagccataaa gactgcctag aatgaatttt tgaaatccta acatcttgat
     6301 tgctttaccg tccgtagatg gtagcaactc aatgaattta atcgctttgg tggcttgttc
     6361 ttcatcgaac acataaggga agctatctga cttagaacgc tccaaatcgc ttatatgacg
     6421 ctcacacgct tggataattt tcttgggtgc aatcagctta cctgatagga ctttctcaat
     6481 gtaatcattc atgaattcat caactctttg aacgggtctt cgggttcatc atttttaacc
     6541 ttgttcaaag ctagttttga tcgtgattct aaggtcatac ctagtgaggt ggcttgtttt
     6601 ttcaaatcac tcatagcttg tgattgaacc cgaaccgctg gattggcttt ctttactccg
     6661 ttttcgcatg tgataaacgt acctgatttt cttatctcgg cttcggctgt tttcattcga
     6721 gcaaaggcta aacaataggc taccaatgaa ccataatctg cttcactcaa tggataatct
     6781 ttttttaatg ttggtacgat acgattccat tcacttctag cgctcgctgc catccagtca
     6841 gggggcgtga tatttattaa tggttgctgc ttgaacaatt cttctctagc gtccacacgg
     6901 attgcttttt cttcacttga tatattgcct tttgtatcgt gtaatatttt agggtgtccc
     6961 atgttgtcct cctttcagac aaaagaaaaa ggaacgtaag caattaagct cacattcctt
     7021 cgtttttcga tcagaatttt tctttatcta atttatctat ataaatgatt tttccgttct
     7081 gcatttgaat ggtcacttga ccaaaatgag gtaactcaat ggtgcgctgt ttccctgttt
     7141 tatcagtaat aacaattgta tttttcaata aatctcctac tttcattata tactttttca
     7201 cttgtgatta caagtgtaat cgttatttgt aattaccctt atgccttttc acaacggtta
     7261 tttgtaccaa aatgagtagg catcgattgt cagtaagttt cacactatgc ccccttaact
     7321 caaagggtac tcttttctcg tctttgtctt tcattggctg tcttggaatt atggtgtgca
     7381 tggcataaag aacgaaaatt agtttcatct aaccgtctat tccaatcatc ttttagttca
     7441 attttgtgat ctacaacatc agctttcctt atcaagcctt cttctaggca atcctcacat
     7501 actggatgat ttaaccgata caagtatgaa gcctttcgcc atgttctact ttgatagaat
     7561 tgaaaatatt taccttcgct ttgctttctt tcttcacgct taattgccgt ttgctttgct
     7621 ttatgcttcc cacaatactt ctgcctataa tcaatcaatg ttttacatcc agcatgatta
     7681 cattgttttt taactgccat agattaccaa ccaaagcgat ctcgttcgtc agaatcatct
     7741 tgttcccatt ttgatttgta ttcaatataa ggcgaaacta tcttcaagca cgttccgtct
     7801 ttaaaattaa ctttagtgat tttatcttcc tcaaaactaa taaagtctac atcttcttga
     7861 gtgtttgaat actcaatgtg tttctcacca tcgaagtatc taatactttc catatgcata
     7921 attattctac ctccaataaa ttagttatca attcatcatc tgttagtagc aaagccaaga
     7981 acttatcatg gatgtttaaa aacaaattat cctcattcga tgtaagctct cgttgtctcg
     8041 ctcgatcata taggttaagc agacaattaa attcatccat tagctcacct gtatcatcat
     8101 agttgccact atctaccaat gatcccacta ctagcgattg acaccacgct actgactcct
     8161 gcagccaatc aggttcttct tccattatct ttaatacttc tggatcaaat gtggaataat
     8221 cttctgttac caatcccaca ctaaatgcca gcgtgttcat tagaaaccga tccatttcat
     8281 ctgtatattg aaagttcggt gctttgtttg ttgaaataat attgagcatt tactttcctc
     8341 cttgattcgt aacacgtggt aacacctacg taacaggtac ccgttaccac tcaatcccaa
     8401 cggggacaag ggttctagcg ttttggtaac aggtaacggg taaaatccag taacattatt
     8461 ccccagcata ttagctaact atttcaaaag ttttaaggaa ataaggtgtt accccgttac
     8521 caaattgctg tatcccttgg ggcagtaagg gtaaactagt aacgggctag gtgttactga
     8581 cccgttacta cccgttactt tttgtacttt ccagctcgat ttttattttc ataggcattt
     8641 gacttattgt aaaatcctcg tcgtttattt ctttttggca gttgcttccg ttcccaacca
     8701 ggaatactgc taataatcaa ggcaatcttt ttagtttctg catctgatcg accattcaat
     8761 aaatcctttg cttgtttatc aaaaacagct tccaaaatat cagcggtgag tacgctatca
     8821 actaaataaa actcttgttg catagtagct ccattataga aatgctgtaa atttttctca
     8881 ttacatttac tgtcaggata gttctgataa taagaacgtc tcgcccacgc tggagcttct
     8941 tcccatttat acgggatttc catttccaaa tatttagtga ttgcttcttt aattggattc
     9001 tccatcttgg catcctcttg atagtctttt gcaatttcta agacttcttt atcttcatcg
     9061 ggactaaaat agattcgttg tcctttatca aaaagcacct ttgcttcagc taacacttga
     9121 agaaaatatt catcctttgt tttaaacgga ttcatctttg gcttattctt acaaggcaaa
     9181 ggataaaaac ggcggtttcc tgtatcatct ttcaaatact gcccatcatt cgacgttcca
     9241 ataaatacac attgccttgc ccaagctatg acattccgac cataaggagg tctaatttta
     9301 tcgatcttgg cactgatgaa gttttttatc tgttcaattt tagttttgtt cattgaagat
     9361 aattctccaa gttcaataat tacattgcct tgaagctgca tataatcatc tttcttttca
     9421 cctagcgaat ctaatgaatc ggtgaaaaaa tcatccgtgt aaagtaatgc tggaacggta
     9481 ctcttaccaa tcccttgcgg tccatccaaa actggaacga tctcaaactt tattcctggt
     9541 ttatagatac gtgcgactga tccgactatc caacgttttg ttacttctcg tgtgtaaagg
     9601 ttgtcgtcta cccctaaaag gtcaataaac agtgtttcca gtctaggctg ttgatcccat
     9661 ttaacttttt cgatacgttc ttttactggg ttgaaaacat tctttctagc cacatttact
     9721 actgctttgt gtattttgtt gtcatcaaaa actacattgt agttttcttc aatatatgcc
     9781 gttaaggcat cctcaaaact gtcttcccat tctccagcgg tacgattgat ccaaggagaa
     9841 tcttttaata ggtgaatgta tccgctaaat tcgttataag caattgttcc agctaaattg
     9901 ctatcattgg ttaaaatcaa ttctgcattc acaacactgt tacttttaat ctgatcgtct
     9961 ttattgcatc ttaatcgtga catccaggtt ttcaattcaa tgatattgtc ttcacgctcg
    10021 ctggcttcgg gaaagtctaa aatactatct aatttatgct tatgagccat gagctttccc
    10081 cctcaaatct tttttaatca tcgatcgaat aatttgattc acttctttat cttttagcgg
    10141 tggttcattg tacgcatttg ctgtataaat caattctgct aaagtttgat tatctacacc
    10201 gtgccatagt aaccaaccgc ataacttagt taaatgatcg tttctactac cttcgccagc
    10261 gccttgaaca atcatatcta atttttttgc tgtccctgtt tttgctacgg gtcggtattg
    10321 agtctttatt tcttgcggtt tttttagcag taggttcact agccaatcgg gagctggtgc
    10381 aatattattt agagatacgt tttcaatagg ttcatatgct ctcccaacac ttcttgaaat
    10441 aatcatagtt ggcgctattg gaactccttt agtgataata tcaatcccgc ttaaactttt
    10501 acctttatca tcacgcccga agatatcagc aaatgcacct gtttcttgtt ggataattac
    10561 accatcagga attttaaaga agaaatggat tcctttacct ggtgtgattt ccgtataagt
    10621 agaaggaaat ggatcataca ttgaagcaag ttttttataa ttctcaacgc cgtttcgtgt
    10681 ttcagaatgt tgatctaggt caactaccac catttgatga tcttcaagca ttaacccaat
    10741 atttctagtt gcatcttccg tccaccattc attaatttga ttagaatcgt tagttgcatc
    10801 attccaacca ctagagcctt ttattggaat tttagttcca ggagataagg gataaattga
    10861 ccatcccgac ttttgtaatt ccgttgctgc ttgataatta tttgccaata aacctctcct
    10921 cctttttcaa aatactggta taaatcattc agacacctcc atgagcttca actgctcaag
    10981 ttcgtatttc aacccatccc gaacaaattc agcacctttt aattgattct gatactttac
    11041 ccatgtatta aatgttttca cccttgcttc ttgcatatca gtaatgttta accatgcggt
    11101 taaaatctga ttttttagct cgttgatttc tcgacaaatt attttgtgtt ctgcatgttt
    11161 tttgagtaca tacaaaactg ttagtttttc attgaatgaa tttccaaagt aggttttccc
    11221 gatattattt tctagttcag cgatcccttg attaatttcc tctaattcaa ggctatcgag
    11281 ttggtttaat agttttgttt tgtacatcta cttatgccct ccagttctta attgatagtc
    11341 gtactcgtca taagcagtga agagatataa cgctaatcct gcaaagatag ctatttttgc
    11401 aattaatggt aataagccga aaattccgcc gattaaaatg cttcctataa ttaagtttcg
    11461 tttcataatg gttccctccg attttttaat atggtataat cggaacaaag aatatcgggt
    11521 tttatcccaa ttaagttgcc tttatcctgt ccgccaagac tgttatggat aaggcttttt
    11581 ctttgttctc gatcatgtta atgctcctaa gttgtctgca attcagcaga acgattataa
    11641 attttctcac tcactgaaaa ctgcaaccca tatttttctt taacagtcat taattctact
    11701 gtctcatcta aaataggttc acgatcaacc aacatttcag gagtcatatc tttctttttt
    11761 accattttgg aatgaccata aatagttgat acagctttat tggctatcac attcgcttta
    11821 atgtagtcta ctggttttgg ttcagtaata gcgtggctta atttagtcat tgcttctttt
    11881 tggtgttctt tgtctagcat gcggaatact tgaaagcctt caagtcctgt ggactggcgt
    11941 agttctttga tgatgtcgaa aatccagtct tgaaatgcct cggcttcttt tttacggctt
    12001 ttgaatgcca tgcggtaaat attttttttc gttgatgatg tttacatctt gtgttctgcc
    12061 caatgaatcg atgaccttac tagtagtaac cccatcttta ggcaaagagt gaatggctct
    12121 agtaacctgt ttaagaccta acgcttccgc aacatctttc gccactgccc accattcatc
    12181 attaatatcg acaaatctta taatgtgtcc gttccaaatt tcagttttca ttcaacgtcc
    12241 tccagttcaa gccctagcaa gctcgctaac aactcaactt gctgatacaa aatttcttga
    12301 tactcttcaa tagaaagtcg ttctttaaac tctgcttgta ctaaatcgtt caagccacgc
    12361 aatactccgc ttaccgttgg ttcttgtggt agcaattttt gatagtccat ttgttcgtac
    12421 aaacattcaa acgtttcttc tagcgctttg ccttccaagt ttgctaattc tgccgctgtg
    12481 tcttctgtaa gaattgttga taatactttt tcgattgctt tattttgtgt cgattccatt
    12541 attatttcct cctattaatc cctttgcccc cacggcttgc gtttattttt ggtattcttc
    12601 cataaagcga tcacattcat cttgtgaaat tcttacgact ccatcaacta taatcaactt
    12661 taaaccgtta ggaacatatt tctgcatcat agtattgtat gaaacgttca tatacttagc
    12721 agcttgtgcc ttgttcatat attttggaaa ctggtacttt ttttctaata atgaaagtaa
    12781 cgttccttga gtttgctcta cttgttgaac aactcgatcg tcaataattt taaaagctgg
    12841 atcatcaagc gtgagtttgt caatttttaa ttccatattt atcccccctc gttagcatac
    12901 atttctatac aaagatttta aaaaaatgca agaggctctt gaatacattt ctatacctct
    12961 tataaactca atataccatc ataccttttc atttgcaaga attttaacat acttttttga
    13021 acttttatgt ttcacacata tttaaatcgt gatatagtag agaaaatata tgaatggtgg
    13081 tgaaaatgtg gctagaaaaa aataccccga agcagtggag acaggacaaa gaattagact
    13141 ggctagaatt gaaaagggac tctcaatgga acaacttgga ggtaaattat ccccaccggc
    13201 cagtaaaggt gccgttagta attgggaaaa tggctataat cttcccaata acaccaggct
    13261 taaacaactt tcaaaggtat tagatgtatc cactacctat ctattaaacg gatcttatac
    13321 attaagagat atgcatatga tgcccgaaat taagcgaaat gtgcttgaaa agcaattgaa
    13381 aaaaactatt gaaaaaaata actctataat tgattctaat ctttctaatg ctatcaataa
    13441 catcgaagaa gataacctga ctgtctcaca gaagatttat ttaacaaacg caataaaata
    13501 tcttaaaaca ggatctcgaa ttgaatttcc tgacgggagt accccaattg cttatctcgc
    13561 agctcttcta aatacgcttt tacgatatga ctcattaatt aatgattcag aaaaaaatac
    13621 agatgagaaa aaagagtatc aagaatttta tgaaaatgat cttctaaaag agttcacagc
    13681 tcttgttact gagctaaata aacactattc aaaataataa taaactgcct cctccaaccg
    13741 cccccacggc ttgctacact atggagggaa aactattatg gcaacattca agcaatatga
    13801 aactaaaaaa ggtaaatttt ggctctatga agcctatctt ggaatgaaca aaatgactgg
    13861 caaacctgat aaggttcgta gacgaggtta caaaacaaaa aaagaagccc agcttgcctt
    13921 gtcacgcttg caagtggact acgataaaaa cggtattaaa aaagcgaaca acgaaacgtt
    13981 taaagaagtt tatgacttgt ggctagaaac atataaaacc actgtccgag aagccacctt
    14041 catgaaaact gaaatcaagt ttagaaagtg gattcttcca aaatatggaa atcttcgtgt
    14101 gaatgaagtc acggtaaaaa aagcccaaca aattgtaaat agctgggctg aaactactga
    14161 ccaatacaag gtacttcatt caacttctaa gcgtattttt agctacgcta tcaatcttgg
    14221 gattattgag actaatccac tagacaacgt attaatgcct aaaagaacca ccactaagca
    14281 taaagaaaag gtcaaagttt acagtaaaga acaactgatc accttgtttg attacctaaa
    14341 cagcaaacct gaaacttatc gaaacgactt tgataaaacg ttactacgct ttctctttta
    14401 tagcggggtt aggatcagtg aagcccttgc attgaattgg tcagacattg attttcaaga
    14461 aaatacggtc accatcaata aaacactaag ccaatccaaa aatggctata aaatcagtga
    14521 tcctaaaact gattgtagtg ctggggttct tccactagat gaaagaacaa tttctatatt
    14581 aaaaaaatgg cagattaatc agcggaaata tatgctgaca cttggtgtaa ctgatccgac
    14641 tatgattttt tgtggtatct ataaagaact aatcacacac cacgctattt atgcaagatt
    14701 gattactatt tgtgaaaaag ctatggttcc atttctcggt gtgcatgtag caagacacac
    14761 tcatgcttca atgctcttgg acgctggagc tactatggta gaagttcaaa ctagattacg
    14821 gcatacaaaa attagtcaga cgatcgattg ttacggtcac ttggcaaaag aaacgaaaga
    14881 aaagactgtt gaaaaattag tacaacactt aaatattgac tagatggcat acaatatggc
    14941 atacagtaaa aaataaaaaa aggagaaacc ctatcatatc aaggtttctc ctagctttat
    15001 agtctattct tcttcttccg ccatgaagtt aagctcatta aagataattg acgatagttg
    15061 gtatttcaac gatttttgaa tagaactaat cactttatta agtctaattc accttaagtg
    15121 gttttttagt cgtattcaaa aacgtattca agttgcgtgg cgtgcttttg ataagaaatc
    15181 gtaagaacta gttaatcaaa cgacttataa ataatgtaac accactgatt aatcagctat
    15241 atccaaaaaa acaagcattc attcagatac atcaatctct atagaataca aagctttcat
    15301 tatctcaaac cattaccaca acttggcaag tcggttaatt gaagaacacc tcattctcca
    15361 cttatagcag tggagcaaca agttgagcta ttgttgcaac agaagctaag aattctgtac
    15421 tccatttaag tttttttaga ctatctaccg caaacctcag catagttttt ttaggctttt
    15481 ctttaatagc ttcatcttta atggtttcga tatactcctg agcctcttct acctcttctt
    15541 caggtaagga atcattcaaa ttctccaaaa gcaaatcaag ctttgataat aattccgaca
    15601 tatcatcaat agaaacaatc atattattat tgtggccttg ctgtatatta gtgttgtaac
    15661 tatttccaaa tatatttact ttattatata ttgaattgtt gctcatatag tcctccagtc
    15721 gtcctgctgc attacttgca tcttgtaacg tcacaaaaaa atctctccca tgttggtaaa
    15781 ttgattcaac gatttcaaat tctggatttc catattcgcg ccattcgtat tcttccttaa
    15841 agtattttcc gtaactatgg ttaaattgca cagaattttt accaattttc cttagaagtt
    15901 ttatctctct ctcagaaata aaattttcct caaaagctga ttcaactatc tgaaaatcac
    15961 taagaaatgt tcctaaacag aagattgatt cattaatagt ttgttgaaaa ttccatggta
    16021 ttttgttcca aaaaccattc ttaattaaag cctctttttt ttcaacaatc tgtgatttta
    16081 cattttctgt atatatcttc caattattga cgtcttcctt aatgtccgaa atcgattgac
    16141 cttcataaga agtccctcca ccaaaaagtc cttgaaccat tttatctcat cctctctata
    16201 ataattactc taaatccaat acttgctaca aatgatagca tttcgtgtgg taacatgatt
    16261 gccatcgttc ctgttttatt tactctgact tcaatacttg gtttgttgat gaaattttac
    16321 caattgattc catcgatcgt ttcatggaat ctgtatttac tattttcatt gattgtagag
    16381 attgatttat tccctctgtt gcacttctga tacctttagt atcaaagttt tgcatgattg
    16441 ctcgctgagc tgataaagct gataatgttt gacctattga ctctatcgac ctcttcatgg
    16501 aatctgtatt taatattttt attgattgta gagattgatt tattccctct gttgcccttc
    16561 tgataccttc agtatcaaag ttctgcataa tttcttgctg gactgataat gtttgaccta
    16621 ttgactccat cgactttttc atggaatctg tatttaatac tttcattgct gatagagact
    16681 ctgacaattt agtaacacct gctaaatcga tattactaaa ataatttcca atatatcctt
    16741 caaaatcatc tgacatatca ctaaggtcct caccagcaat atctccagcc aatatttttt
    16801 gaaaagcttt ctctagttga tttgtatatc tcttcaattt ctttattaat ttttggtata
    16861 aagccatttc tatttctttg ttatggctta caatattcct accttctctg attaaactca
    16921 tgtctgtttg aatttcttct agcttaacat ccatatattt actaaatact tcactccaca
    16981 aagaatatga atcagatata gtgtgactgt tatcttgaaa taacttcaat aattgatcct
    17041 gagatatttc tttatacctt acaatattct cttcattttc ttgtgtaact aaaatataat
    17101 tttctccaaa gagatattct tcaaaatcat ataaatctat ccattgtaaa agattctttg
    17161 agtcaaactt tcctattgct tgtatttgtt ttctttttat tgacgaaatg atttcatttg
    17221 gaattgattc ctctgcccag ttatcagaaa aatatgccgg agctaataaa tagattaatt
    17281 ttcttaatgc ccattcgtaa ctatgcaaat acggtaatag ttttttcgaa aaaaacaacg
    17341 catttgtatc taaaagggta gtgtagttta attttgattt acttgcttca atattgacct
    17401 tagctaggaa aatttgaaat atatgtacct cttttattcc tatcttacca gtcttattat
    17461 attgaaatac tacgtgtaac gaatcattat agttctttat gtcaaagcca aatttataat
    17521 ttttttcctg ctcctgaaat cttaagatag aatcactaat atatggtgac aacactttca
    17581 gaaaggtttc ttcgatattt tccattcttc cttttttagg ataatataaa tattcaacaa
    17641 cttgcttact aatatgtcat caacttcttt cctttcaaca attttaattt tcctattaac
    17701 ttgatatttg ctacataagc cacaactagc aaacagaaag agaacttttt ctttaccttg
    17761 ataactgtgt taattactgg tttctttttt ctatttcttt atagtattgt tcttctactt
    17821 ttaatatttt tttaacttct ctttttatgt cagcatacat agatatactg ttgtcctttc
    17881 ggttgaagca gtgataattc tcaccatcaa actcataaaa aagcatatca cttgagcttc
    17941 catctgaatt aattaactga aatttggtat tgagtagtaa ttcatttttc tcgtcattca
    18001 actttattaa aataggtttg atctctttta aataattgaa atatatttcg gttattacta
    18061 aattaaattt cgctactgta taaacataag agatacgtgg ggaatctcta aattggttat
    18121 atatatcttt ccttaaaaca tctaactcct tttttaattg tccgttaaaa tcaaaatgag
    18181 gcattgctag aatttcatcc aaatcaaagt acactctttg ttgatgaata tttactggca
    18241 gatgaccatg ctgagaataa tttctcatat gtaataaaaa cctatacgaa aaa
//
LOCUS       DANKHL010000040        18223 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000040 DANKHL010000000
VERSION     DANKHL010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.042"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            39..1700
                     /locus_tag="PW101_000961"
     CDS             39..1700
                     /locus_tag="PW101_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2513378.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1770..2117)
                     /locus_tag="PW101_000962"
     CDS             complement(1770..2117)
                     /locus_tag="PW101_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDL2513379.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2201..3142
                     /locus_tag="PW101_000963"
     CDS             2201..3142
                     /locus_tag="PW101_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2513380.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3143..4186
                     /locus_tag="PW101_000964"
     CDS             3143..4186
                     /locus_tag="PW101_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2513381.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4192..5262
                     /locus_tag="PW101_000965"
     CDS             4192..5262
                     /locus_tag="PW101_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2513382.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5252..6205
                     /locus_tag="PW101_000966"
     CDS             5252..6205
                     /locus_tag="PW101_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2513383.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6217..7227
                     /locus_tag="PW101_000967"
     CDS             6217..7227
                     /locus_tag="PW101_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2513384.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7512..8333)
                     /locus_tag="PW101_000968"
     CDS             complement(7512..8333)
                     /locus_tag="PW101_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2513385.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8430..8708)
                     /locus_tag="PW101_000969"
     CDS             complement(8430..8708)
                     /locus_tag="PW101_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513386.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8976..10985
                     /gene="metG"
                     /locus_tag="PW101_000970"
     CDS             8976..10985
                     /gene="metG"
                     /locus_tag="PW101_000970"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDL2513387.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11464..12051)
                     /locus_tag="PW101_000971"
     CDS             complement(11464..12051)
                     /locus_tag="PW101_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513388.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12226..13548
                     /locus_tag="PW101_000972"
     CDS             12226..13548
                     /locus_tag="PW101_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2513389.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13703..14479
                     /locus_tag="PW101_000973"
     CDS             13703..14479
                     /locus_tag="PW101_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL2513390.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14472..15041
                     /gene="rnmV"
                     /locus_tag="PW101_000974"
     CDS             14472..15041
                     /gene="rnmV"
                     /locus_tag="PW101_000974"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDL2513391.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15034..15918
                     /gene="rsmA"
                     /locus_tag="PW101_000975"
     CDS             15034..15918
                     /gene="rsmA"
                     /locus_tag="PW101_000975"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDL2513392.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16369..16998
                     /locus_tag="PW101_000976"
     CDS             16369..16998
                     /locus_tag="PW101_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513393.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17122..18222)
                     /gene="ugpC"
                     /locus_tag="PW101_000977"
     CDS             complement(17122..18222)
                     /gene="ugpC"
                     /locus_tag="PW101_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003406299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDL2513394.1"
                     /translation="MALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGKSTT
                     LRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGLKLR
                     KYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMDEPL
                     SNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIGTPK
                     EVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGYEGK
                     EVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKVDAR
                     DFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
BASE COUNT         5943 a         3293 c         3738 g         5249 t
ORIGIN      
        1 ttaattattc tgaaaattcc aataaagggg tacgtaaaat gaaaaagaaa tttacatttg
       61 gtttggttgc ggtatgcggc cttgtacttg ctggatgtta tggcggcagt tcagccacaa
      121 gcagtagttc agcaaaaagc ggttcagcag atggaggagg cgtatttaac ttagtcgtgc
      181 ctcaagaaat gcctacagct gacttatcag ttgcaacaga tacaatcagt tttacagcat
      241 tgaataacgt ttatgaagga atttaccgtc ttgatgaaga cagcaaacca cagccggcag
      301 gagctagcga gttggcagaa gtcagtgaag acggattaac ttacaaaatc aaattacgag
      361 aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc ggattacgtt tatggctggc
      421 aacgaacagt agacccagct acagcttctg aatatgctta cctgtatgcg ccagttgaaa
      481 atgcagaaga cattacagct ggaaagaaag ataaatcaga actagggatc aaagcagttg
      541 gtgactatga attagaaatc aaattgacga aacaaacacc atatttccaa tatctgcttg
      601 ctttcccatc atttttccca caaaaacaat ccgtagtgga agaaaatggg gatgcctatg
      661 catctgcaag tgataaagct gtttataatg gaccattcac attagaagat tttgatggac
      721 caggaacaga tacagaatgg acttataaga aaaatgatca ttactgggac aaagatacag
      781 taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc ttctactgca ttgaacttat
      841 tcaaagatgg ccaagcagat gacgtgatct tgtctggtga gttagcacag caaaacgcaa
      901 acgatccagc ttatacttct gttaaagaag cgcggacaag ctatatcgaa ttcaatcaac
      961 gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa agctatttct tattctatcg
     1021 atcgtgaagc tttagtaaaa caagtactag gtgacggatc agttgtctct acaggattga
     1081 tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga ctttgcaaaa gaagctggaa
     1141 aattagtttc ttatgacaaa gataaagcaa aagaatattg ggaaaaagcg aaaaaagaat
     1201 tgggcgttga ttctttagaa tttgatttga tggcttctga tgatgattca tcgaaaaaag
     1261 taatcgaata tgttcaaaac tcaatccaag aaaacttgga tggcgtaaca gtcaaaccaa
     1321 cacctgttcc attctctgta cgtcttgacc gctcaacttc gggtgatttt gacgcagtac
     1381 taggcggatg ggctgctgac tatgcagacc caagcagctt tactgatttg tttgtcacag
     1441 gtaattcata caaccgtggt cgctggagca atgcagaata tgacgaagca gttaaagcag
     1501 cttcatcaac agacgctggc gatgaacaag cccgttggga tgatttccaa aaagcaatca
     1561 agatcatctc tgatgatatg ggtgtcgttc cggtgtacca aaaagcagaa ggtcacctag
     1621 tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg cgcttcatgg gattacaaat
     1681 ggacatacgt tgaagaataa tcgttaatac cgtgagcatg agaaaaaacg aggctgcggc
     1741 aaatgtcgca acctcgttct catattcact tatcctaaca aagaaaaaag catgaaaata
     1801 gcagaaagag caattagtat tccggcaatg atcagccaaa agctgtacat accgtagctg
     1861 gcagacgaga gcgtggaaaa ttctggtttt ttctttcggt tccttgttcg tgcaagatgg
     1921 accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga cccataaaaa accgccgata
     1981 atcagaaacg gcaatgacca taagaataga tcattagata attgaatgag tgtaaaggaa
     2041 tggttgatga gattttttat taaaataacc agaatcagta gaaaagctac tgcgtagggg
     2101 atagattttt ttttcatttg attgctcctt tatttttggt tcattatcta ataatgtagc
     2161 gaaacatctg tcagaagtca aaagctaggg ggaaacatta gtgaatagtt atatcaaata
     2221 tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg ttaattgcaa caatcacctt
     2281 tttcttgatg caattgctgc cgggcacgcc atatacgaat caggaacgtt tgagtcctga
     2341 aacaatcgag atgctaaata aacaagtcgg tttggataaa ccagttatcg tacaatatgg
     2401 gatttatctt tctaatttgc ttcaaggaga ttttggaatt tccttccaat ttaaaaacca
     2461 gccagtcgca catttgttag ctggaagaat cggaccatcc ttgcagttag ggttacaggc
     2521 aattatcttt ggaacagtcc tagggacgat tttaggaacg atctctgcca tgaagcaaaa
     2581 tacatgggca gatacatctt ctacactcgt agcgatatta ggtcgttcca ttccaaactt
     2641 tgtatttgct gttttattac aatatatctt tgcaatcaaa ttacaagttt tgccaatcgc
     2701 aaaatgggat ggctttgtgt acacaatctt accaacaatt gcgttggcca tgtcgccgct
     2761 tgctgactct gctcggttta tccgtacgga aatggtggaa gtactgcata gtgattatgt
     2821 ggaattagca aaagcaaaag gattgagccg ttgggaaatc gcctttaaac atggtcttcg
     2881 aaatagtttg attccattga tgactttatt aggaccgtta gctgtagcat tgatgaccgg
     2941 atcactggtg gtagaaaata tctttgctat tcctggtatc ggggaacaat tcgttaagtc
     3001 aatcacaaca aatgattatc ctacgatcat ggctgtaacg atcctttact catttatgct
     3061 gatttttgtt attttgatcg tggatctatt gtacggtcta gttgatccaa gaattcgtgt
     3121 ttcagaaggg agccgaggat aaatggaaat gataccaaag cgttatgaaa cagttgcaga
     3181 tatccctgcg gatgaatttc ttccattaca aaaaaatacc gaagaagaac gtgaacagat
     3241 tactgcacct tctttgaatt ttatccaaga ttcatggcgc cgtttaaaga aaaacaaagc
     3301 tgccgttatt tcaatggttt tactattggt catcattttg atctctgtga tcacaatctt
     3361 tgtttctcct catgatccaa ctgcccaaaa cgtggcttat atcaatttgc ctcctcgtat
     3421 tccgggaatc aatatcgatg gattgaatgg aaaagcaatg gttggtggtg aacttgttga
     3481 taaatatgct caagcaaacg tcccttcaaa cgtaaatttc tttttaggaa cagacggttt
     3541 gggacgtgat gtattaagcc gcttatttat gggaatgaga atctcgttgc tgatcgcatt
     3601 tattgctgca ttactagatg taacgattgg tgtagcttac gggttggttt ctggtttgtt
     3661 aggcggacgt gtcgataacg tgatgcaacg ttttttggaa atcttatcag gaattccaaa
     3721 cctcgttgtc atgattttga tgttggtcgt ttttgaacca ggaatttttt caatcgtggc
     3781 tgctatggcc atcacaaact ggataccaat ggcacgtatc gttcgtgcac aaacaatgaa
     3841 attaaaagat caagaatatg ttttagctgg attgacatta ggtgaatcaa gaatgaaaat
     3901 tgcattcaaa catattttgc cgaatatctc tagtgtcatt attattcaaa tgatgtttag
     3961 tattccatct gctattttct tcgaagcctt tttaagcttt atcggtttag gtcttacgcc
     4021 tccgtcagct tcattaggaa cgatgttgag cgaagggtac aagacattcc tgtatctgcc
     4081 ttatctatta tggattccgg cagtaacttt atcggttatc atgattggct ttaacttact
     4141 tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa gagtgaagac gatgacgaaa
     4201 attttagaag tgaaagactt agaaatctct tttgatacgt atgctggaaa ggtacgagcg
     4261 attcgcggtg tcaattttca tttgaataaa ggagaaacat tggcgatcgt tggggaatct
     4321 ggaagtggaa aatcagtgac gaccagaagc atcatgcgct tgctgtcaag caacgccaat
     4381 atagatgctg gtcaaatcct ctttaaaggt aacgatatcg ttggaaaatc agaaagagag
     4441 atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc aagatccaat gacctcttta
     4501 gatccgacga tgcccattgg gaagcaagtc gctgagtccc ttagaaagca taataaagtt
     4561 tcaaaaaaag agggacaaaa agctgcatta gaattgctta aattagttgg aatacccgaa
     4621 gctgagaaac gtatcaacag ttatccgcat cagttctcag gaggtcagcg tcaacggatc
     4681 gtgatcgcga ttgcactggt atgttatccg gaaatcctga ttgctgatga acctacaaca
     4741 gcgcttgatg tgactattca agcacagatt ttggagctgc tgaaagaaat tcagacaaaa
     4801 atcgatacat caattatctt tatcactcat gaccttggtg tagtagcgaa tgtcgctgat
     4861 cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg gaacatcgga agaaattttc
     4921 tacaacccgc aacatcccta tacttgggga ctgcttggtt caatgccgac attagatagt
     4981 gcgaacgatc gattgtatgc tattcctggt tctccaccgg atttattgaa cccgccaaaa
     5041 ggagacgcat tctatccaag aaatgaattc gcgatgaaaa tcgatgcaga acaagaacca
     5101 ccattttttg aactatcaaa aacacacaag gcagcaactt ggctacttgc tccgcaggca
     5161 cccaaagtga caccgccaga agaaatccaa cgtcgctggg ctatctttaa agagaaacaa
     5221 aaacatactt acggtcaagg aggaattgct gatgccaaat aaactacttg aagttaaagg
     5281 attgaaacag tatttcaatg taggaaaaaa gaatgaagta catgctgtaa atgacatcag
     5341 ctttcacatt tatgaaggag aaacattcgg tttagttgga gaatctggaa gcggaaaatc
     5401 cactactggc cgtacgatca ttcgcttgaa tgaacctact gatggtgaga tattattcga
     5461 cggtcaagat ataacgaaga tcaaagataa aaaaggtttg acgaaattcc ggcatgatgt
     5521 tcagatgatc tttcaagatc catacgcttc gttgaatcct cgtatgaaag tcagagatat
     5581 cattgccgaa ggaattgatg ttaatggact agctaaatca tctgaagagc gtgcaaaaaa
     5641 agtcgatgat ctattgagaa ctgttggatt gaatccaagc cacggtactc gttatcctca
     5701 tgaattttca ggtgggcaac gccagcgtat tggtattgca cgagcattgg cagtaaaacc
     5761 gcgttttatc atttgtgatg aaccaatttc tgcactagat gtgtcgatcc aagcccaagt
     5821 ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg acctatctat ttattgcaca
     5881 tgatttgtcg atggttaaac atattagtga ccgaatcggt gtaatgcata acggcttgtt
     5941 attagagatg ggaacaagtg atgaaatcta taatcacggt gttcatcctt atacagaaag
     6001 tttgttatct gctattcctc taccagatcc agaccatgag agaaaacgca gaagaatcaa
     6061 atatcaacct gaaccagatg acggacaagt acgtcaactt cgtgaaatcg caccagaaca
     6121 ttttgtctat gcaactgagc aggaagtccc atattacgca aaaaaactaa aacgacaaaa
     6181 ggaatctctt ctggttgcta actaggaggg acatagatgc taaaagcttt taaattccgt
     6241 atgtatccga cagaagaaca aaaacagcaa ttgattcgta cttttggatg tgctcgattt
     6301 acctataacc atttgctgaa aaagcggcag aaaagttggc aacaaacagg agtagccaat
     6361 ttttcgctca cacccgctac attaaaaaaa gaatacccat tcttaaaaga agtagacagt
     6421 cttgctttag caaatgcaca gcttaatttg gatcgggctt ttcgcaatta tttcaaggga
     6481 cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt ggcaatctta tacgaccaac
     6541 aaccaaaccc gtacgatcta tctggaaaat ggctacttaa agttaccaaa acaaaaagaa
     6601 ctaatcaaga taaatcagca tcggccagtt gaaggatcga ttcgctccgc tacgatttct
     6661 gctcgatata atgaagaatt ttatgtcgca ctactttgtg atgtttcgcc agtaaaaaaa
     6721 gaatctttag ctaaatggat cgggatcgcc tatcatccga agacattgat ccaaacatct
     6781 cgacctctag aagtaacttt accaaaattt caccagacag aagaaaagct gcaacatgca
     6841 cagcggaaat tgaatgtgaa agtccgctcc gctcaccata gaaagatcag gttagatcag
     6901 gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc tttatctgaa acaaaagaac
     6961 cagcgagaag attatcttga acaattatct gggaaattgg tcaaacaata tgactactta
     7021 tttgtagaat cttttccaaa agaagaggca catgctgatt tttctattca cgactggcat
     7081 aaattaataa caaaattgcg ttataagtcc caatggtata ataaaaaatt tcttttgatc
     7141 aataccgacg gagcagaaga aagcaattcc gtaagaaaga gccaagtagt agaaaaaatg
     7201 ggcagacatt cggttatcaa gggataaaaa atgaatgaaa ctgataggag cgttagggtt
     7261 agccttgtta ataaaaagtc ttaatgactt ttgtttctag gaatttttct tttgaagaaa
     7321 agctcactaa ttatgacaaa ccaataaaac aatagcagaa aaagttcgta cgagcttttt
     7381 ctgctattgt taatttttta ttcagaaagt ttacgaatac atcaaaattc tgaaacaaaa
     7441 aacgtaactc acaaagagtt gtttttgcgt tttttcttga atgattaggt ctgaatcttt
     7501 tgctcacaac cttattcatg gtgccccggc ttgagctgat agatttttga aggacggccg
     7561 attcctacag gacgttcacc gatctcatcg aaatattgga gcatagcttt cttaaaattg
     7621 gaatgatcga tcgttttata atcgatccct aagaatttag caaatacttt tcgagcttcc
     7681 gtaatcgtga aatctttacc taaaacttgt agaacttgcg gctcgtgttc cattttattg
     7741 actacacggt tgaatgcttt caagatgatt tgactatggt caaaagccaa agtatctttg
     7801 ccagaagact ctccagtgac taaatcaagc gagatagcca cttctccgct agataaatgg
     7861 atcttgttac tatgacgttc tagtgtgaac cagcgtactt ccttggcatc atctccggcg
     7921 attagcggtt cttctccaat aaaggctaag tagctgactg tgacgaccca gcctcgcgga
     7981 tctcgattag gcgtactgaa tgtatgcagt tgctcaatat tttcttttga aataaccaca
     8041 ccagtttctt cctttgtttc tcttaagacg ctttctccag ttgactcatc cttttggaca
     8101 aagccaccag gcaatgccca tgaatttcga taaggatgcc cttttctttg gatcaacaaa
     8161 actttcagct gatcttcttc tttgttatag cacatcagta cgatatcgac agtcaaagag
     8221 ggcttgtcat attctggacg ttcctgtttt tgataccagt taagaaattc ttcttcactt
     8281 gctttatgtt cataatataa tttttcttct tctttagaag caaaacggtt catctgacca
     8341 cctccattga cagtaaaagt actttttacc ttattgtagc agactctgaa aaaaaaccaa
     8401 ggatttactt gaaaaatcta ggttatcatt taaatatgaa aaataaatgg aagtatgaga
     8461 ttgaataaaa agaaaagcag ataaaacggc aatactttat accgctcttc acgaatggca
     8521 aagaagccac aaataacgat atatacgtca atgataaaac gcaaaatcca agctgctgtc
     8581 ataaaactgt tcaaatgccc atggaggtta aaataaccat acatacgaat ataaaaatag
     8641 ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa gtgtaaaaaa aagatgtttc
     8701 ttctgcatga ttgttccagt cccttactaa gatagatagt ctttattata ccacgtgtga
     8761 ggggagaaat aaaaagccgt tttttagtag atatgaaaca aaacaaaaac ccgcatttat
     8821 tcaacctatt catcctttta aaggatagat tgaacgtaga tagaagtcgt ttatccagtc
     8881 tttttcattt tgaaaaaaag ctaacagcat gcttttttta tggtatacta gcaaggaaac
     8941 gaattcgcat catgcgataa aggaggaaga gccccgtggc agaaaaggaa actttttata
     9001 tcactacgcc aatctattat ccaagcggca aacttcatat cggcaactcg tatactacga
     9061 ttgcatgtga cgcaattgct cgttataaac ggttgatggg ctttgatgtg ttttatctca
     9121 ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc agaagaacta ggtgtaaaac
     9181 cgcaagaata tgtagataaa atggcggctg atgtcaagaa attatggaaa acgcttgata
     9241 tcagttatga taaattcatt cgcacgacag atgattacca taaagcagct gttcaaaaaa
     9301 tatttgatcg tttattagaa cagggagata tttacttagg tgaatatgaa ggctggtact
     9361 ctgtttctga cgaagaattc tttacagaaa cacaacttgc agaagtgtac aaagatgacg
     9421 aaggtaacgt gatcggtggt aaagcgccaa gtggacacga agtagaattg gtaaaagaag
     9481 aatcatactt cttccgcatg agcaaatacg cagaccgtct attggaatac tatgaagaac
     9541 atcccgaatt catccagcca gaatctcgta aaaacgaaat gatcaacaac tttatcaaac
     9601 caggattaga ggatcttgca gtttcacgaa caacttttac ttggggagta ccagtcaaga
     9661 gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt atccaattat attactgcac
     9721 taggatatgg ctccgaagat gaaagtctat tcgaaaaata ctggcctgca gatgttcaga
     9781 tggtcggtaa agaaatcgtt cgctttcata cgatctattg gccaatcatg ctgatggccc
     9841 ttgatttgcc tctacctaaa aaagtatttg ggcatggctg gctattgatg aaagacggaa
     9901 aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat gcttgtggaa cgttatggtt
     9961 tagatgcact ccgttattat ttgcttcgtg ctgttccgtt tgggtcagat ggtgtcttta
    10021 cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc aaatgatcta gggaacttat
    10081 tgaatcgaac gattgctatg atcaacaaat attgtgatgg actcgttcct gactatgcct
    10141 cacttgtcac tccatttgat agtgaactgt ctacgacggc tgctaacgtt gtcggacgtt
    10201 accatgatgc aatggaaaaa atggaattca atacagcatt ggctgaaatc tgggtgctga
    10261 tttctcgtgc aaacaaatac attgatgaaa cagaaccatg ggtattagca aaagacgaag
    10321 aaaagaaagc tgaattgaat agtgtcatga tccatttagc tgaatctttg cgtatcgcag
    10381 cgatattact tcagccaatc atgacagaaa cgccagagaa gatcttcaat caactaggat
    10441 tggactctga aacaatgaat cttgaaggat tacactttgg cgaatttcct tcaggaacaa
    10501 aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga tatggaagaa gaagttgctt
    10561 ttattcaaga aaaaatgtct gaaggtactc aaacaaacga agatacagtc aaatgggatc
    10621 cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat caaatttgat gtatttgaaa
    10681 aagtagaact aaaagtagca gaagtcatca actgtcaaaa agtagaaggg gcagataagt
    10741 tgctgcaatt ccgactagat gctggagaca gccaagaccg tcaaatcttg tcaggaatcg
    10801 ctgaatttta tcctgatcca agcgaattga tcgggaaaaa agtcgtcatt gtagcaaatc
    10861 tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat gatcctttct gcagaagcac
    10921 cagatggctc tttacaagta atcgaagcac caaaatccat gccaaatggc tcagaaatcg
    10981 catagaaatt caaagggggt atgacactag ttgtgtcata cccccttttt ccgaaatttg
    11041 agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc aatcatataa tacagaaaca
    11101 gagaactatc caaaataaat cctagaaaaa gaagtacgag cttcgctgat ttttgttaat
    11161 ttagaataat actaatagga atcgtaaaaa cagaaaaaaa gcataatgat aaagggtcgg
    11221 gcagatttta attgattcat ctggtactct tttttattgt ccttacattc ataaagtttg
    11281 aatgcaatga ctttattaca tctatattta tatctaacag tagcgttgat gtgttaatta
    11341 taagttctat cactttatgt tcaattagtt tgtgctctta tatgtgtgat tgaaaatata
    11401 gataaaaaaa caatcatcgg ctttgacttc gatgattgtt ttctttattt tgtaaaaatc
    11461 ctatcacttg attttatggt aaccttttat ttttgtcttc aaacggcttg gtaagagagc
    11521 agtaagcagt gcaaatggga tcgaaaagac ataccagaaa atcgtacgtc cgataccata
    11581 atgttgatag agctttgtca acatcacgtg taacaagatc aaccctaaaa gcaaggagcc
    11641 agctataaac caatgattat cccaatgcat gacttcacta agtgccatca ctccaaaagg
    11701 atagaaataa aaatagatcc cgactaaggt catccgccag ttctttgtaa atgttccgat
    11761 attcaataaa aagaaaatcg ctgaaagcaa aataccgata ggcggaaaaa gcccaaccaa
    11821 aaaggaatac aacatccccc acaagatcat cacaggacct ttgatcaatg caacgattgc
    11881 gatgaacaaa gtcagcaata ttagtttttg tgataacgtt gcaacagtca ttaatccaaa
    11941 taaaattaat gtgaaccaat gccaatggga ctgctgttgt tctttacggg aaaaatcgac
    12001 ccggttggat aaataatctg ctaatttcgg ataagaacgc ttaaaatcca taaactgttc
    12061 ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa aaaagaaata catccgtctt
    12121 aagagggagt ttttcactga aagtggagaa aaaagaatgg taaattaaaa tattctcagt
    12181 tatactgaaa attataccaa atttcaaaga agaaagagga tgggaatgaa aggcaaacta
    12241 aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta tgggaaccgt gatcggtgca
    12301 ggcgtcttct tcaaaactgc tgctgttact gctagtactc agtctacaag tttgacattg
    12361 ctagcttggc ttttgggtgg tttcttaacg atctgtgctg gattgacagt ggctgaatta
    12421 gctacagcaa tcccggaaac tggtggagct gtaaaatata ttgaagctgc gtatggaaaa
    12481 ttgcccagct ttttattagg atgggcgcaa agcttgattt actttcctgc taatattgct
    12541 gcattatcga ttatctttgc gacacaattg acgaacttat tacaattatc taccgattat
    12601 cttttgttga ttgcagtgat caccgctgtt tctgtgacag ggttgaattt gttagggaca
    12661 aaagtcggtg cttctgtaca atcagtgacg ttgattgtta aattaatacc aattgctgtg
    12721 attgtcatat ggggattatt gaccccagga caaggaactg tccagttatt tccaatcgaa
    12781 gctggaaaag atgttacttt tgtcgaagga ttgagcagtg cgttgttagc aacgttgttt
    12841 gcttatgacg gctggttagg tgtcggtgca atggcagggg aaatgaaacg gccggaaaaa
    12901 gatttgccta aagcaattat tcttggtttg agttttgtaa cagtcgttta tctattgatc
    12961 aattttgttt ttttgaaaac tttgccgatt gatcatcttg ccggtaattt gaatgcagct
    13021 tctgaagcat cagacgtcat ttttggagga atcggtggaa agctggtcac tattgggatc
    13081 ttgatctcgg tatacggtgc attgaacggt tatacactaa ctgggatccg cgtgccttat
    13141 gcgatggctt tagaagacga gctacctttc agtaaacagc tgacaaagct ttcaaagaag
    13201 tttacggttc cttatgttcc tgcggttttc cagttagcag tagcttgtat tatgatgagc
    13261 cttggttctt tcgacttttt aacagacatg ttgatttttg tgatgtggct attcagttta
    13321 ctgattttta tcggtgtctt tattttaaga aaaaaagcgc cggaattgcc acgtccatat
    13381 aaagtaccac tttatccaat cgttccgatt attgcgattc tcggtgctat tttcatttta
    13441 ggtatgacga tgatgacgca aacgaaatta gcattgatcg gtataggtgt taccttgata
    13501 ggtatcccag tctattatca aaaaaagaaa aaacgctcag aagagtaaaa tatttcaaca
    13561 acagacagaa aaagtacgaa ctaggtcctc tagatcttcg tgctttttct atttcaataa
    13621 aacaagttct tctattttga tttttgctac ttctgcaagt agtgcttatg tgctaaagta
    13681 agaatgaaaa ggagagataa acatgatatt tgattctcat acccatttaa atgctgaaca
    13741 gttcaatgaa gatattccag aaacgattca gcgagcacaa gaaatggggg taacgaaaat
    13801 ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct ttgcaattaa gccatgacta
    13861 tccaaatata tacagtatta tcggctggca tccgacagag gctggcagtt atacgaaaga
    13921 aatcgaaaat aaattacaag aacaattgac tttaccaaag gttgtagcat taggggaaat
    13981 tggcttggat tatcattgga tggaagaccc gaaagaagtc caagataaag tatttcgcag
    14041 acagattgct atcgcaaaag aaatgaacct accaatcagt attcatacta gagaagcact
    14101 tgaagacacg tatcaaatcc taaaagaaga agatgttcga gatatcggtg gaatcatgca
    14161 tagtttcagt ggtgattatg aatgggcaaa acgatttctt gacttaggga tgcatatttc
    14221 atttagcggt gtcgtcactt tcaaaaaagc attagacgtc caagaggctg cagcaaatgt
    14281 accaatcgaa cgattacttg tggaaacaga tgccccttat ctggcaccag ttccttttag
    14341 aggaaagcga aatgaaccag gctatactcg ttatacagta gaaaagattg ctgaacttcg
    14401 gaatcttccg acagaagaag tggctgccca aacatggaca aatgcgcacc gattatttgg
    14461 tctaagcgaa catggataag atgaagatcc aagagattat agtcgttgaa ggaaaagacg
    14521 atacaagaag gctgcgggaa gtagttgatg cagatacgat agaaacaata gggtctgcaa
    14581 tcaatgaaga gatcctgatg caaattgaac atgcccaaga gactcgtgga gtgatcgttt
    14641 ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat cattatggaa gctgttcctc
    14701 aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc aaagaaaaag ggaagccttg
    14761 gtgtcgagca tgcaagtgat gctgctattt tagaagcatt gaaaaaggtc gtgacgccag
    14821 acatttccca aacggctgtt tctgaaatca ctagacaaga ccttttagac tatggtttaa
    14881 tagctggggc acaagcaaaa gaaaaacgag aaaaactagg tgacgagttg cgcatcggtt
    14941 acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt tcgtattacg ccgaaagaac
    15001 tcgcccaagc aatgaaaata gtggaggaac aacatgagtg aatatcgtga aattgcgaca
    15061 ccctctagaa caaaagagat tttattgaaa catggttttt catttaaaaa aagtttagga
    15121 caaaattttc tgactgaacc aaatatttta aggaaaatcg tagaaactgc gcaaatagat
    15181 gaacatacga acgtgatcga agttggacca ggaatcggtg cattgacgga acaattagcc
    15241 aagcatgcaa aacaagtcgt agcatttgag atcgatgatc gtttgatccc tgtattggct
    15301 gatacgatgc aaccctatga caatgtgact attgtacatc aagatatttt aaagaccgat
    15361 ttgtcaacag ctgttagaga aacatttcat gaggagcttc cgttaaaagt cgtagcgaat
    15421 ttaccttact atattacgac accgatcatg atgcattttc ttgaatcaga tttggttgtg
    15481 gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc gtatctctgc tgaaccagga
    15541 acgaaagctt atggttcatt atcgattgct gttcaatatt atatggaagc cagtttagca
    15601 ttcatcgtac caaaaacagt atttgtccct cagccaaatg tagattcagc gattttgaaa
    15661 ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg aaaaagcatt tttccgattg
    15721 acaaaagcgg catttcagca aagaagaaaa acgctttgga ataacttgca gcattcgtat
    15781 ggcaaagatg accagacaaa agcatggtta gcaaaaagct tggaaacagc tggaatcgat
    15841 ccaaaacgtc gtggggagac tttgtcatta caagaattcg ccgcattaag caatgcgatg
    15901 tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt tgtgaaaaag ctgtcctgca
    15961 tcaagtaata agaacagcca aacaaaaata gttcctcatg tttttcgttt gtttgggctt
    16021 attaccgcag atgcaagctt ttgaacaccg tttaagaaag aggttgtggg cctgccgttt
    16081 aactctaagt cacaaggaac aatttcaaaa aacagcttct catgtttttg agttagttgg
    16141 tcttattact tgatgcaagc ttttgaccac tattatgccg aacaaggaga tgtgacacaa
    16201 attttgtcac atctcctatt tttgtttctt gatcaaaaaa ttcttatact tgatactcac
    16261 actcattttg tatggattta gaaaagaata tgtgtcttga cagttattag taagtgagtt
    16321 atactaagtc gacatttatg tgtcaagaca tattaggaga gagaaaaaat gagaaaaaat
    16381 tcagttcgaa aactaacgat ttctgcttta ttgattggga tgggggtcat tatcccaatg
    16441 gtcatgccta aaatcatgat tccaccagct tcctttactt tggcgtctca tgtgccatta
    16501 tttattgcga tgttttttac accaggagtt gcagtggctg ttgcgctagg tacgacattt
    16561 ggtttctttt taacaacgcc ggttatcatc gcattgcgag cattgtcaca tcttgtgttt
    16621 gcgttgattg gggcttggta cttgcaaaag catccgagta tcgttttaaa aaatggacag
    16681 tttacttttg ctaattggcg tttccaaggg ttcaattttg tgatcggtgt gattcattct
    16741 ttagcagaaa tgctagtagt cagcatcttt tactttattg gtaatatgcc tgaaacatat
    16801 tattctcaag gatattttta tacagtattc atcttgatgg ggattggtgg gcttatccac
    16861 agtttagttg attataatat tgcttatttt atagcaggaa cactcagcaa atattttgat
    16921 atccccgtat ttacggcagc aaaaaaagaa aatcggaaag agtcgattca agggtacgcg
    16981 aagaaaacga catattagac atgatctctc aaaaaaaaaa ctctcatcta gtaatggata
    17041 ataaatcctc taagatgaga gttttttgtc ctaaaaagag ctgcaccaac atcttctggc
    17101 acagctctaa cagggggcaa tttatggaag tttgatgact tgttctgttt ctttatcaaa
    17161 gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt tcgcctggtt tatggaaatc
    17221 tcttgcatct acttttgaga tgaactcagt agaaccagtt tttgtgtaaa gcatcgtttc
    17281 agctccaagt aattcggaaa cgacaacttc tgatttcact acggcttcag gtgaagcttc
    17341 aagggctact tgttcactgt ggatgtcttc tggacgaata ccgaagataa cttcttttcc
    17401 ttcataacct ttttcaacta atactttgtt ttttccttca ggaatacgaa gcttaagccc
    17461 atgtccatct gtgataacac catttttcaa tgtcacatta aagaagttca tggcaggaga
    17521 tccaataaag ccggcaacga acatattgac gggtgtatca taaacttctt tcggtgtacc
    17581 gatttgttgg ataaatccgt ctttcatgat aacgattcgg tcagccatag tcattgcttc
    17641 tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga cgctggtgaa gtttggcaat
    17701 ctcagcacgc atggctacac gtaatttagc atctaaattt gataatggct catccattaa
    17761 gaagactttt gcatcccgaa cgattgcccg tcccaatgcc acacgttggc gttgtccacc
    17821 agataaagca gctggttttc gtttcaagta ttctgtcaga cctaagatct ctgctgcatt
    17881 gtccacacgt tttttgatgt ctgctttatc atatttacgt aatttcaagc caaaagccat
    17941 attatcaaaa acagtcatat gaggatacaa tgcgtagttt tggaaaacca tggcaatatc
    18001 acggtcttta ggtgccacgt cattcatgac ttttccgccg atatttagtt ctccttcaga
    18061 gatatcttcc agtcccgcga tcattcgcaa agtagttgac tttccacaac cggaaggacc
    18121 aacaaaaacg ataaattcac ggtctttgat tgatagatta aaatcagtta cagaataatt
    18181 ttctgcattg tcatattttt tatatacgtt tttcagagcc att
//
LOCUS       DANKHL010000041        18102 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000041 DANKHL010000000
VERSION     DANKHL010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.043"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(240..1037)
                     /locus_tag="PW101_000978"
     CDS             complement(240..1037)
                     /locus_tag="PW101_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL2513395.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1034..2218)
                     /locus_tag="PW101_000979"
     CDS             complement(1034..2218)
                     /locus_tag="PW101_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL2513396.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2243..3490)
                     /locus_tag="PW101_000980"
     CDS             complement(2243..3490)
                     /locus_tag="PW101_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL2513397.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3705..5450
                     /gene="dnaX"
                     /locus_tag="PW101_000981"
     CDS             3705..5450
                     /gene="dnaX"
                     /locus_tag="PW101_000981"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDL2513398.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5470..5784
                     /locus_tag="PW101_000982"
     CDS             5470..5784
                     /locus_tag="PW101_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDL2513399.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5809..6405
                     /gene="recR"
                     /locus_tag="PW101_000983"
     CDS             5809..6405
                     /gene="recR"
                     /locus_tag="PW101_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDL2513400.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6469..7113
                     /locus_tag="PW101_000984"
     CDS             6469..7113
                     /locus_tag="PW101_000984"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDL2513401.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7128..7457
                     /locus_tag="PW101_000985"
     CDS             7128..7457
                     /locus_tag="PW101_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDL2513402.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7471..8409
                     /gene="holB"
                     /locus_tag="PW101_000986"
     CDS             7471..8409
                     /gene="holB"
                     /locus_tag="PW101_000986"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDL2513403.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8445..9269
                     /locus_tag="PW101_000987"
     CDS             8445..9269
                     /locus_tag="PW101_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDL2513404.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9262..9609
                     /locus_tag="PW101_000988"
     CDS             9262..9609
                     /locus_tag="PW101_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDL2513405.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9678..10550
                     /gene="rsmI"
                     /locus_tag="PW101_000989"
     CDS             9678..10550
                     /gene="rsmI"
                     /locus_tag="PW101_000989"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDL2513406.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10659..11780
                     /gene="dinB"
                     /locus_tag="PW101_000990"
     CDS             10659..11780
                     /gene="dinB"
                     /locus_tag="PW101_000990"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDL2513407.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11834..12436)
                     /gene="nrdG"
                     /locus_tag="PW101_000991"
     CDS             complement(11834..12436)
                     /gene="nrdG"
                     /locus_tag="PW101_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDL2513408.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12751..14940)
                     /gene="nrdD"
                     /locus_tag="PW101_000992"
     CDS             complement(12751..14940)
                     /gene="nrdD"
                     /locus_tag="PW101_000992"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDL2513409.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15239..16033
                     /locus_tag="PW101_000993"
     CDS             15239..16033
                     /locus_tag="PW101_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2513410.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16023..18065
                     /locus_tag="PW101_000994"
     CDS             16023..18065
                     /locus_tag="PW101_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2513411.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         5641 a         3327 c         3770 g         5364 t
ORIGIN      
        1 atattgtgac ttgtgaccga ggagttggta ggagaacacc gtttaccaag aggttgtgaa
       61 aaagctgttc tgcatcaagt aataagaaga gccaagtaaa aacagtctct tatgttttta
      121 acttggctgg acttatcacc gtagatgtaa gcttttgaac gccgtttatt tgcaacaaaa
      181 ggggctgtga cacgattttt gtcacagccc tttttttgcg aacattcatt tagcaatttt
      241 tagaaacttc tgccgccacc gccaaaagtc ccgccaccgc tagaatgggt agtacttccg
      301 ccaccgccgc ttccaggagg tgggcttttg gggattcttc ttgtcgtgac aaaagaattt
      361 gtcagacgat cggttttttc agttaatttg atactggatt tttctcggaa tggatatttg
      421 tagatgcctg atttcaattg ataacgcgaa acagtcacaa aataaaagac aagactaagg
      481 ataagagcca aaacaacagc aatgatgatt tctactgtcg tcaaaacctt atagcgtgta
      541 attttgccag tttcttcgtc tacacgataa tgtcctcctg gaactccgtc ttccacatat
      601 gttgctgatt tagacaggta agcttttgca gcattgtaat aggcagcatt tgtcatctga
      661 tcataaacat catctaagat cgaatcgatt ctgctatcag tcaaataatc gatcatatta
      721 ccagaagtcg aaatgtagat ttcacgctgt cccatatcta atagcaatac tgagccattt
      781 tgatcatttc cgactgcgtc tcttaagtaa ttatcagcaa agtctcttgg ttcttctgaa
      841 ttagatgtag tcgttacgat caagactcgt cctttgattt tttcattcag cttttctgcc
      901 tgttgttcca aagattggat ttgatctgtt gtgaataacc cagcctcatc atttaccgta
      961 gctgtatctg catgagtagc caccggccag ccaacactta ctagcaaaag aaagaataaa
     1021 aaaaattttt tcatcatatc aagtaacctc cgatcagaaa gaggatcgct agcccagcga
     1081 aaatcccaag agcggtcagt cccaattttt tctggctgat cggcaggaca ccgctgacct
     1141 ttcctgtttg tccattcatt gcgtagtagt agactttttt acttgagtca ttgcttcgat
     1201 aagtaacaag ccataccggc agtaatacgt aattatttac ttcgtttgtg attgaagcag
     1261 aagttcgcac accggtcaat gttgtatatc catttgcggt atcttttaac agcttctctg
     1321 aatagtcttt taattcagat tgaatctgtg atttgattgc ctcatattcg atatctcttt
     1381 tttctgcttg gaacccggct aaatactggc ttttaaaatc aattgcttta tctaacggga
     1441 aaggttgcac gccttcgacc atcttttgtt gcgtattttt tgataaggca ttttttacta
     1501 gttctttaaa agaaagcttg cctgctcttg aaacagcaaa ttgcttcgtt tctgtgtatt
     1561 cgatgtcacc tatccgccat atgcggataa ctgtaccatt cgcctgcatg gcaccatcta
     1621 cttcagcatc tgtagcccaa taagggaaat acacacctgt caacttatcg atttgatcct
     1681 tattgaaaaa agctttggga acaaaccatt ttttcttggt ccaagcgaga aatctgttga
     1741 ttgcttcttc tttctcgaca gcaaaaggaa gtactttatt tggcaagaat tctccgctta
     1801 aacgtcccga tagtacgacg ggattatggc agtagtaaca ataagtcgca gcagttgtcg
     1861 catcagtcac gatctctgca ccacagttgg gacaaagaaa aagatctatc gctgcggtat
     1921 tatctgcaga ttcgtccgct gtatctgaaa aaccgcctgc ctcgtcaacc gctttgcgct
     1981 cggcttcatt catatcggca agctgttctt gggctgtaaa agtaaaatct tcggtaggtg
     2041 aaggttcttc tttgctttct tcgccagcca tacgcgcttc tttttgcttt tgctcatatt
     2101 ggctgacttc ttcttctgta tagatattca ggcagtattc gcaatgaaat ttttggtctt
     2161 tgggatcaaa agtcaacgga ccgccgcaat taggacattt atgtgtaaga acatccatca
     2221 gctgcacacc tttctaaata aattaattaa cggggattcc tttaaacggt ttaccacagt
     2281 ttggacagaa tttagggatg ccgttggaaa gatccacgat ctcattgcat tcactgcatc
     2341 gcatttgtag cttaggctga acgttttcta caggtttggc agctccgcag ttggaacaga
     2401 attttccgga attttctgtc ccgcaaactg gacatgtcca agtatccgca tttccttgtg
     2461 cagtttgttg attttgtttt tctgcttgtt gtttgatttg ttcttggtta ttttgagctg
     2521 cttgagaaag ataagaacca tttgcattca ttcccatacc tacacccata aagccggtca
     2581 ttgctcctgc ttcatttttt ccggctgctt ccataccacg tgcaatggaa ccttgcacgt
     2641 agccttcacg tacgctagga tcacctagca tcgccccttc gttacgcata ttgatcagtt
     2701 tcacagaatc atcggtgtaa gaaatgctgg ctacagccac agaaacaatc tccatcccac
     2761 gtaattcacg ccaagaatca tccaatgcag tatccatgta tttgcttaat tccaaactct
     2821 tagatggtac ataagaaatc cgttgaccat ctgcagacat ctgattgatt gctgattgta
     2881 gcgctgttag gaattcagct aaatactgct cattgatatc attgatttcc acttgtgttt
     2941 tgtttttagg gattgcgttt gtataaaata aaatcgggtc aacgacatga atagaataag
     3001 tgccatgtgc ccgtaagaat aattctgcgt tatagaaatt gtcaaagtag ttcaacggcg
     3061 cgcttgtacc gaatttgatt ccctttattt cttggagatt gatataaaaa acttgttgtt
     3121 tctgcggtgt gacgccgcca aatttgaatc ggtcaaaagt ttcagcaatt gcttctttca
     3181 gtgttccatt gaacatggaa ggagctgcgt catttttaat ggtgtaataa ccttcctcag
     3241 cggtataatc gatgattttt ccaccatcga ccaaaagcat catcatgttt ggatagacat
     3301 ggacgactgt tccgtctgtt aaaacatctt ctgttccttt acgattggag ccacgtttgt
     3361 catctttgcg gactttgaca ccttttgtca tgaccgttgt atcactcata ttgtctggtt
     3421 cgatgacttc tagccattga tctgccagac cgccgccaac catacttgtt gctgctttga
     3481 taagacccat aaaaaattcc tcctctgatt cagctagtgc caaacaatta ttgtttattt
     3541 attataccat aggaagatgt gaaacttctc ggactagaga cggaattgaa atggaaaatt
     3601 gcgtattgtt taaatacctg aaaaagccga gaaggatagt agttttttta cctaatatca
     3661 cgtataataa gagatagtga attttttaaa aaagggggat ccttatggca tatcaagcac
     3721 tttatcgagt ctggcgttcg cagcgtttcg aagacatcgt tggacaaaaa gctgtgacac
     3781 agacactaaa aaatgctatt gtttcccaca aaacctcaca tgcctacttg tttacaggac
     3841 ccagaggaac cggaaagacg agtgcagcaa agattttcgc aaaagcaatc aactgtccga
     3901 acagtaaaga cggtgagcca tgcaacgaat gcgaaatgtg tcgatcaatt actgctggta
     3961 cgcaagaaga tgtgatcgaa atcgatgctg ccagcaataa cggagtcgaa gaaatccgat
     4021 ttatccgtga tcgggcaaat tatgcgccta caaaggcagc gtacaaagtg tacattattg
     4081 atgaggtaca tatgctttca acaggagctt tcaatgcctt gttgaaaaca ttggaagagc
     4141 caaaagaaag tgtcatattc atcttagcaa cgaccgaacc tcacaaaata ccagctacga
     4201 tcatttcacg gactcagcga tttgatttta aacggatcaa tacggcagat attgtcgaac
     4261 atcttgaatt tatcttgaaa gaatcgacta tcagctatga accagctgca ttgaatgtga
     4321 ttgcccgttc tgcagaaggc gggatgcggg atgcattgag tattcttgat caggcaatct
     4381 cgtttagtga aggaacaatt actttagatg atgccatgca agtgacaggt agcttaactg
     4441 acgaaatgat ggacagttac ttgagtgctt gcgtgaaaaa agaagtacca gaagcattgg
     4501 aaacattgaa ccaaattctc gcagccggca aagaatcacg tcgctttcta gaggatatgt
     4561 tacaatactg ccgtgacttg ctgatgtatc agcaagcacc taacctagtt gaggaacaat
     4621 cgagtacgat cagaagtaca tttacagaat tggcacaaac tgtttcagcg gatcagctgt
     4681 tttcaatgat aaagattttg agcgatactc aaaatgaaat tcgttttacc aatagtgccg
     4741 ctatttattt ggaagtcgct acagtcaaac ttgcacagct accgtctgct tctgttcctt
     4801 cagcagatgt agcatttgca gatgatcaga tagtaaaaca gctacaacaa gagattaagg
     4861 aactaagaaa cgaaatcaag gaactacgca gtaatacagg aattgagaat acaaaagtac
     4921 agccggtaca gcaaacaaat cataaaaaac agacaagtac ctatcgtgta ccgacggaac
     4981 gagtatataa agttctgcgg gaagcgacaa gacagcatct gaaccaagta aaagaagtat
     5041 gggaagactt gctgatgagt ctatctgtca ctcagcgagc aatgcttaaa gcaagcgaac
     5101 cagttgcagc tggacctaca ggactagtga ttgcgtttga ttatgagatt gtttgtcaaa
     5161 gagcagcaaa tgacgaagaa ttgcagcttt cggtacacaa caacttaagc cgaatgatca
     5221 aagattatgc gcctgatcct gtttatatta caagagaaag ctggccagaa cttcgaagaa
     5281 attatttgac gcaggcacaa gaagagggaa cagaaaactc ttttgcttct gaagactcag
     5341 aaggagaaat cgagttgctt cctcaagaac aagaagtacc agaagaaaca cttgtagtaa
     5401 aagcagagga actgtttggc tccgaagctg ttacagtggt agatgattaa taaaaaggag
     5461 tagataatta tgatgcgtgg aatgggcaat atgcaaggca tgatgaaaca agtacaaaaa
     5521 atgcaaaaag aaatggcaga agcacaagag ctgctaaata aaacagagtt tacaggtaaa
     5581 gccaccaatg aattggtcaa agcggttttt acaggcgatc gtcgcatgaa agatatccaa
     5641 atcaatgaag aagtcgttga tcctgatgat atcgagatgc ttcaggatct tgtcgtaatg
     5701 gcagtcaatg atgcgatcgc acaaattgat gtggaaactg agaaaaaatt agggaaatac
     5761 gcaaaaggtt tgccaggatt ttagttttaa actgaaggag taatgaatat gcaatatcca
     5821 gaaccaattg cgaaactgat cgacagctac atgaaacttc caggaatcgg tcagaaaaca
     5881 gcgacaagac tcgcctttta tacgatcgat atgaaagatg aagtagtaaa tgaatttgct
     5941 aaatctttgt taagtgtaaa acgcgatttg catttttgta gtatctgcgg aaacatcaca
     6001 gaagaagatc cctgcgaaat atgtagaaat ccttcacgtg atcgaagcgt tatcctagtg
     6061 gtcgaagagc cgaaagatgt aatggcaatg gaaaaaatgc gagaatatca cggactttat
     6121 catgttctcc atggtgtgtt atcaccgatg gaagggacag gtccagaaga catcaatatt
     6181 tcttctctgt tgcagcggct gcatgatgca gatgtaaaag aagtgattat cgcaacaaat
     6241 gcaacaacag agggagaagc aacagctatg tatctctcac gtctgatcaa acccgcaggt
     6301 atcaaagtga ctcgtctagc acatggtctg tctgtaggct ctgatatcga gtatgcagat
     6361 gaagttactt tattgaaagc tgtcgaagga agacgtgagt tataaatttc taattaagtg
     6421 ttttttcaga tacaatataa aagcagcaca agagatgggg gcaacaatgt gaacggacta
     6481 tttattacaa tcgaggggcc tgatggtgca ggaaaaacca gcatcttgaa tgaactttac
     6541 cctcttttga aagaagtagc aaaaacagaa atcattcaaa caagagaacc aggaggcatt
     6601 ccgattgctg aagagatccg agcagtgatc cttgatccaa aaaatgatcg gatggatgaa
     6661 cgtacggaag cactgcttta tgcagcagca agaagacaac atctagtcga aaaggttctg
     6721 cctgctctgg caaaagggaa aatcgtttta tgcgatagat ttgtcgacag ttctcttgct
     6781 tatcaaggtg caggccgacg catcggcgtt accgagattg ctcggttgaa tgagtttgct
     6841 acagaaggta ctacgccgga ttttaccctt tatcttgacg tagattcaga caccggatta
     6901 agaagaatca agaaaaacag acaaaatcaa atcgaccgtt tagattcgga aggtttggaa
     6961 ttccaccagc gtgtacgtca tgcttactta aagctggcag aagagaatcc ggaaagaatc
     7021 cacaaagttg atgcaagaaa aagctttgaa gaagtgctgc aaacaagtta tcatacaatt
     7081 attgaacagt atccgcaatt ttttgaaaac taggaaggtg aacgtttatg aaaattattt
     7141 tagcgattat ccaagacaaa gacagcaacc gtttatcaaa tgaattgatt gatgcaaata
     7201 tccgtgccac aaaactatca tcgacaggcg gcttccttaa ggctggaaac agtacgttta
     7261 tcgtgggtat tgaagatgac cgtgtagaag aagcattgga aatcatcaaa aaaacgtgtg
     7321 aatcaagaaa gcaatttgta tctacacctg ttacactgga tatttcgatg gatgggggcg
     7381 tcccttatcc agtagaagta gaagtaggcg gtgccacagt cttcgtctta cctgttgaag
     7441 gattccatca attttaggag gagctgagct atgaatcaag aaataaactt ggctcaaatg
     7501 caaccgatcg tgtatcagca gcttcaacgg agttttgagc atgggcgtat cgctcatgct
     7561 tatctttttg aaggggagaa aggaacagga aaacatgaag taggtatatg gcttgcacag
     7621 cacctgttct gtacccaaat gaaagacaat ctgccttgtg gaaaatgcaa caattgtcag
     7681 cggatcgaaa agaaagaaca tccagatgtt ttggttattg aaccggaagg acaaaccatc
     7741 aaagtcgatc agatcagacg tttgcagacg gaattcagtc gcagcggtta cgaatcaaga
     7801 aaaaaagttt ttttgatcaa agaagctgaa aaaatgaata gcagtgcggc aaacagtttg
     7861 ttgaaattct tggaagagcc ccctggagat tttttagcgg tgttagaaac agatgcagta
     7921 ggaagaattc tccctactat tcaatctcgt tgtcaagttc tgcattttca agaactttcg
     7981 aaagaagcac tgatccatcg attgcaagaa gagcagattc cattggaaaa agcaaaactt
     8041 ttggctttct taacaaacag tttaggaaaa gcagttgaaa tctctcaaga tgaatggttt
     8101 aatgatgcta aggacttgat ttatcaatgg ttcgtttact tgcaaaagca agatactcaa
     8161 gcctttatct atgtccaaaa aaaattggta aaatcattta aagaaaaaac acaacagttt
     8221 acaggattat ctgtcttgat gttttattat caggaagcac agcgtcaagc agtagcaaac
     8281 ggcgaatatg gcaaagtgaa acagataaac cagataattg aacggatatt gatcgcagag
     8341 cagaaactac gcagcaatgt cagttttcag gctgttgcgg aacagttcgt tttgcatacg
     8401 attttttagc ttgccgcaaa ggatgatgat caaggaggat ggaaatggta gaagtagtag
     8461 gtgtccgata tagagatgca ggtcatatct attattttgc accaggagac tctgaatatg
     8521 tttatagtga caaggtatta gtcgaatctc agcaatcctt acatattgct actgttgtcc
     8581 ttcctaaaaa agagattgat cccaatgacc ttcctgaagc tatccgtccg atcttgcata
     8641 aagcgtcaca agctgaatta gaaaaagaca aaaaaaacaa aaaagatgct tatgatgcat
     8701 tgaaaatcgc aaaagaaaaa gtgcagttgc gtgagttgga aatgaaaatg gtcaaagccg
     8761 aatacacatt tgatcgaaat aaattgattt tttctttcac agcggacggg cgaattgact
     8821 ttagagaatt ggtaaaagat ctggccgctg tatttcgtac tcgtattgag cttcgacaaa
     8881 ttggtgtccg cgatgaagca aagataatag gcggaattgg tccatgtggg cgtccattat
     8941 gctgttcttc ttttttaggc gatttcgtgc ctgtatcaat caaaatggca aaagatcaaa
     9001 gtttatcttt gaatccaaca aaaatttccg gtttatgcgg acgcttgatg tgctgtctgc
     9061 aatatgagag cgaagaatac gagacagcga aaaaagaact ccctgacttt ggaaaagtaa
     9121 ttgctacacc tgatggtaaa gggaaagtcg tagggttgaa tctgcttagc cgtatcgtta
     9181 gtattcggct gactggacgg gaaaatcctg tcgaatatga atgggatgaa ttgaaagaat
     9241 ttatccaagt aggtgagatg aatggataag agatcattat acgacggatt gaatcaatta
     9301 gaatcagagt tgagaaatac attaacagaa cttgttgaaa tgaagcatgc tttgcatgag
     9361 atcgtagaaa aaaatacaac tttggagatg gaaaatcaac gtctgcggga acacctgcgg
     9421 gaattgaacc aagttgcaca agctccagct gaaaatgtga agcaggaact ttctaagtct
     9481 cgtatgaatc ttgaaaaaat ttatgaagaa ggattccatg tctgctacga tttatatgga
     9541 tcaagaaggg acaatgacga gccttgtgcg ttttgtctag aagtgatcta tcgcgaaagc
     9601 ggtcgatgac caataacaag aggagactgg agcggagaaa tactgctcca gtctttttat
     9661 taaggagatt gtgacagatg tataatcaaa aaagtttcaa gggaaatccg gtatatgggg
     9721 atttatatct tgtaccgacg ccaattggaa atttagaaga tatgagctac agaagtgttc
     9781 gtatgctgca agaatccgat cttatcgcaa gtgaggatac gcgtaataca caaaagttat
     9841 tgaatcattt tgaaatccat acaccccaaa aaagtttaca tgaacataat tataaagaac
     9901 gtgtccctca attgattgct ttgttaaaag aaggaaaaac gattgctcaa gtcagtgacg
     9961 caggaatgcc ttccatcagc gatcctggac atgagctagt tttagcctgc ataaaagaag
    10021 ggatttctgt gatctctatc ccaggaccta cagctgggat gacggctttg attgcttcag
    10081 ggctagtgcc tcaaccattt ttattttatg gcttccttcc acgtaagaaa aaagaacaga
    10141 aagaaacatt gaaaaagcta aaagaacaga cagttacgct gattttttat gagtcacctt
    10201 atcggatcac tgctacattg acgaatttct tagaaatttt tggaaatcgc caagtcgttt
    10261 tgtgtcggga attgacaaag gtccatgaag aataccttag aggaagtacg gaagagctac
    10321 tcgcttatat cgaagaacat ccagtaaagg gtgaatgttg tctgcttgta gaagggaatt
    10381 tgttcgaaat gccagtgacg gaagttgatg aagagttggg aacgttaaag gaacaagtag
    10441 aagcaaagat cgcttcaggc gaaaaaccaa atgctgctat caaagcagta gcgctgaaaa
    10501 atgggctgaa aaaacaagaa gtctaccgtc agtaccatga attagactga gatagtataa
    10561 gttgtctata atgaaagact aatgaggaaa tcagctacta acttttcaaa tctcttgaac
    10621 gcgaacgaat gttcttgtat aatgaataag aggtgaggat gatggagctg caatttcctt
    10681 tacataatga tacatcaaga aaaatcattc atattgatat ggatgctttt ttcgcttcgg
    10741 ttgaagaacg agatcatcct gagtttcgcg gacatccatt agtaattgcc agacacccca
    10801 gtgacactgg tgggcgtgga gtagtgacta cagcaaatta tgaagcaaga aaattcggaa
    10861 ttcattcagc aatgagtgca caaaaagcgt atgagctttg tccgcaagcc atctttaagc
    10921 ctggcgacca tcataaatac agcgatattt ccaaacaagt acgggaagta ttctatcgtt
    10981 atacagatat tgtggaacca gttagtatcg atgaagcgta tcttgatgtg accgaaaata
    11041 aaatcaattg caagtcagcc atcaaaatcg cccgaatgat ccaagcagat atttgggaga
    11101 gtgtccagct aacatgttct gcgggagtaa gttacaataa gtttttggcc aaattagcat
    11161 cagactatca aaaacctaga ggaatcacgg tagttgcccc tcaagacgct gtgccatttt
    11221 taaaagcttt gccaattgaa aaatttcatg gggtagggaa gaaaactgta cctagaatgc
    11281 atgagctagg gatttataca ggaaaagacc tatatgaatg cacagaaatg atgttgattc
    11341 gaaattttgg aaaaatgggc tattctcttt atcgtaaagt cagaggaatc catgattctc
    11401 cagtaaacgt tacaagagag cgaaaatctg tgggaaaaga gcatacttat gggcagccgt
    11461 tgcaaacgga agaagcagtg ctgactcagt tgcgccaact agctgaaaaa gtagaagaag
    11521 cactgagaag agtccaaaaa catgggaaaa cagtagtttt gaaggtcagg tacacggact
    11581 attctactgt gaccaaaaga gtgacattgc cagaatatat ctataaaaaa gaagcactct
    11641 tttatcaagc aagccttata tgggaggaaa tcttgggagt agaaaaagga atccgattgt
    11701 tagggattac tttgacgaat ctggacccga tgacttatga aaacatcgtc cttccattat
    11761 gggaaaatca agaaatataa aaacagcttt gagagttgaa accggaaatc cgttttctct
    11821 cccaaagctg tttttatatc aagtcttgct ttttgatttg ttcataggtt tctgttgcat
    11881 ccgtacattt ttcccaaatc acaacttgat tggattctaa agatttttgg acgtcaatga
    11941 tccgctggtt gctacttccg cgaaattgta gttttaggtc acgtttgctt aattcaaagc
    12001 gtccgtcaac aaggatatcg atttgcgaaa ggagttctaa tttatcttct gaatcttgta
    12061 gcaattcatc aaaggtatag cctgtccatg accagatatc ttttgtcgta ccgaactctt
    12121 ttcggatacg tcgtacgact tgcagacaaa catctgtatt caaaaaaggt tcgccaccaa
    12181 gtaaagttaa tccttgcacg taatcgttgc gcaagtcagc gatgatttgt tcttctaatt
    12241 caggtgtgaa tggtttgcca tatcgaaaat tttgtgcagc tttgttgaag cagccttcgc
    12301 aggcaaataa gcagccacta acgtaaagac tgttgcggac accttcacca tcgacaaaat
    12361 taaacggttt gtagtcagca atatagtttt ggctgtagtc ttttgctaac cattcttttg
    12421 gttttggatt tctcataaaa aaacttcttt catttaaggt tcaaagatat agctattgaa
    12481 gataaaatct actcgaccac ataaataaga acaaactgac ctaaatccat tgttcaagaa
    12541 agcttgttct tattttgata gagggcgttc aaagatacaa agccatcgaa agagggtggt
    12601 gaaaaaagac ttgtcatagc ctcttattct gaataaacgg tgttcaaaag cttgcatctg
    12661 cagtaataag cccaactaaa cgaaaaatat gaggaactat ttttgtttag ctattcttat
    12721 tacttgatgc aggacagctt tttcacaacc tcatttcatg tgtttcacac gtgaggaaat
    12781 ttctttatgg cggccatgta ccatcggacg agcttgcgga tttcctaaat agccacaagt
    12841 acgtttgact acatcacatg tttttggatc atgatttcca cattctggac attcgaaccc
    12901 tcgttctgtc ggtttgaaat caccttcgaa accacattga tagcaatgat cgattggcgt
    12961 attcgtacca agatacccga ttttgtcata tgcaaaatcc catacagctt ctaaagcttt
    13021 cggattttgt tggagcatag ggtattcaca gtagtggatg aatccacctg aacaatattt
    13081 tggataatcc ttctcaaatt ccaatttttc aaatggcgtt gggtttttac gcacatcata
    13141 gtggaaactg tttgtataat attctttatc tgtgatattt tcgatgatgc cgaatttttc
    13201 tgtatccaaa cggcagaaac ggtcagttag actttcactt ggagttgaat agacactgaa
    13261 atgataacca tattcatccc cccatgcatc tgcattagct tttaattctt tgacgatatc
    13321 aagtgtgaaa gcttttgctt ctgggtttgt ttcccattcg ccaccgaaga aagctgaagc
    13381 aacttcatac aatccaatgt agcctaacga tacagtagct cgacggtttt tgaataattc
    13441 attgacagaa tcggtacgtg aaagacgttt cccgaaagca ccatacatat ataaaattgg
    13501 cgcatttgct ggaatggctt ctttacaacg ttctacacga tagagaagcg catctttcat
    13561 gatttctaag cggtcagaca gtaactgcca gaatttattc tgatctcctt ttgattcgat
    13621 cgcgattctt ggcaaattca atgtaacaac acctaaattc attcgtccga catttacttc
    13681 ttgaccattt tcgtctttcc acccttgcaa gaatgaacga cagcccatag gtactttgaa
    13741 acttccagtt aattcaacga ttttatcgta attcagaata tcaggataca ttcttttcgt
    13801 tgcacattcc aatgctagtt gtttgatgtc gtaattagga tcttcagggt ttaagttcac
    13861 accttttttc aaagtgaaga tcagtttagg gaagatagct gtccgctttt cactccctaa
    13921 tccattgatt cgaatctgta aaattgcttt ttgaatttct ctttcaaacc agttttctcc
    13981 tagtccaaat cctaatgaag tgaaaggagt ttgtccattt gaagtaaata aggtattgat
    14041 ttcatattcc aaactttgca tagcatcgaa gatatctttt ttggtttttg cttttgcata
    14101 ttctttttgg cgttctggcg aatcgatcca ttcttccgcg tcttttagat gttttttata
    14161 gttcaattct gcaaaaggag caagcaattc atctgttcgg tcggcagaac agccaccata
    14221 ttgacttgac gctacattgg caataatctg tgagatttgt gctgtggcgg tctggataga
    14281 ttttggcgat tcgacttcgg cattgccgat tttgaaccca ttgttcagca tgcctttgaa
    14341 atcaatcaag cagcaattcg tcatcggtgt ataaggatga taatccaaat catggtaatg
    14401 gatatcgcct ttttgatgcg catttgctat atgtggtggc aacattttca gtccgattga
    14461 ttttccaacg atcccggctg tcagatctcg ctgtgtattg aaaacattac tgtctttatt
    14521 tgcgttttcg ttcacgacag tttggtcctt gttgattagc ttttcaatcg aaaagttgat
    14581 atctgttgct ttactgcgag caaaatctcg tttggtacga taattgatgt actcttctgc
    14641 taattcatat tcgttttttt ctagtaatat atgttccacg atgttttgaa tctcatagat
    14701 tttgacattt tcagcaaacc ggcttttgat ttcttggaca accaagtctg caatcatttg
    14761 aattttttca taagtaagtg gtgataatga gccgtgaatt ttcttttctg ctttgatcag
    14821 tgcttcattg atcttttgtt catcgaaatc aacatggcgg ccatcgcgtt tgatgacttt
    14881 catctgggat gtctctggct gctgagtaac gacagttttt tcttcataat ttactttcat
    14941 tatcttcaac tcctgctaaa ttgttcttgt ctatcataaa acaaaatcgg ggaaggagca
    15001 actaaatatc ggctatatat agtattcaat gtgctgtata attggatttc tatactatat
    15061 gtagtgtata ttttattgtg aaaaaaggtg aaaacttgta gctggtggat ttgttttttg
    15121 catatttaat tttgctgaat aaaataattg aataaaattt tttattcagt ccaagtaaaa
    15181 gtgacaaaaa cataagagca aattattcgt agatatgcta ctatgcaaat aaatagaaat
    15241 gaaaaatgaa agaggaggag aagaattgga aaagatgatc gaagtgaaac atctaagtaa
    15301 aagctacggc agcaaacttt ctcccgtcaa agccttagat aatatctcat tcacagtaga
    15361 agaaggcgag tttgttggga ttatgggccc aagtggtgca ggaaagacaa cgctgatgaa
    15421 tatcttgtcc acgatcaatt tgcctacgat gggcagtgtc tcgatccaag gaaaagaaat
    15481 cacaaaaatg aaaaacagtg agctcagtga ctttcgtcga aaaaaactag gattcatttt
    15541 tcaagagttc aatttgatcg ataccctaaa tgcaaaagac aatatcctat tgcccctagc
    15601 agttgaacgg atgactaaag aggaaatgga aaagagagta agacatgtag ctcaattgtt
    15661 gaatatagaa gaactgctca aacggtatcc cgatgaactt tctgtcggac agagacaacg
    15721 aattgcagct gctagagcac ttgttgttca gccacaagtg atttttgcag atgaaccaac
    15781 tggctctttg gactcgaagt cggctacaga attgttgaac tacttgaaga ccatgaatca
    15841 aaaagaaaaa gcaacgatac tgttagtcac ccatgatcca tatacagcaa gctactgcga
    15901 tagaatttta tttattaaag atggtgtgat tttttctgaa gttgtcagaa gagggacaag
    15961 aagagagttc tttgagaaag taatagacat gcaggcgacg attggcggag gaggaaaaat
    16021 gaatgctgta taaactagcc gttaaaagtt tttttcggca gtcacgaggc tatcttgttt
    16081 attttttcag tttgacttta tccgtaatga tctattattc cttcagcgca atgacctacg
    16141 atcaatcttt agtcagaaga gcaagtcaag atacttcgat agatggtgga ttgagttttg
    16201 gtggatttat cattactgtt gttttgcttt ttttcgtatt cagtgccaac cgatttttct
    16261 tgaatagaag gcaaaaggaa ataggcatct atcagctttt tggtatgaat aaactgcaga
    16321 tatcaggtat atatgtgtta gaaatcatga taattggatt gttcgcctgt atttctggga
    16381 tattactcgg tattattttt tccaaactct tttcaatgat tttggttcga atgatggata
    16441 tggatcttac tagtccattt tttatctcca ttccatcagt agtagatact ttatttgctt
    16501 ttttcatcat ccttttagca gtatctgtat attcaatatg gaaaatatgg cgctatccaa
    16561 tgattcgaag ttttggagaa aaagaacaag cagatagttc aacaatgcgc ttcaggacaa
    16621 ggcaccgatt actcgcagtc atcgggttac tacttttaac gagcggatat tttggagcta
    16681 gtcattttag ggaaatcact tcatggatga tcagttatca ttatcttgat acattagttt
    16741 tcttttttcc ttttttaatt tttttcgttt gtgtggtcgg tacttatttg tttttctgtt
    16801 atacatttcg gttatttttg atcatattga gtaaatggaa gatttattac tatggtatca
    16861 attttttgat aatgggtaac acgcaagttc atttgctcaa aagttggcgt acaaattctc
    16921 tgattacagt cattctcgga atttctttaa tgacaattgg cggcacggct agtatctcta
    16981 ctattttatc ctaccaggag aaagtttcct ccccgatgga ctatcagttt gatgttgaga
    17041 cagagaaaaa aatcaagccg attctagcag aggaagggca gaagataact aaagaaatca
    17101 ccttgcatta taaagtcatt gggagtatct atagtcaaaa tctcgaagaa cctatgtttc
    17161 aaactgctaa tctgatcacg gaaacacaat atcaagcctt cagaaaagtt aatccagatt
    17221 tgccgaacat ttctttgaaa tcacctggtc atactgtttt gcttgatcaa atgcaaactc
    17281 tttttcggaa tatttcatta tatggatcta gtattggact gccagataat caagaattga
    17341 agattcaaca aatgcttcca agtgtattag gtgacgaaac aatggtatat ctaagtcctg
    17401 tactgattgt agatgatacc gtttttgaaa aagcaaaagg tgtggattat caagtagtca
    17461 atgtagatgt atcaggtgga aataatgaaa aaatagatga acgactgagc aatgacctgt
    17521 cgataaagtg gctcaatgct gtttattatt cttatgatat cgtaaatgga agtctacaag
    17581 gagaaattag cacaaagaaa cttccggatg accaaatgaa ggaagcacaa tttagccaag
    17641 aaagctctcg tttgaatttc tcttccagct attcgaaatt gcgtgcagtt aatcggcgaa
    17701 tcggtatcaa tatttttgtc acactatttg tcgggatgat cgtgattatt gctactggta
    17761 gtattttgac agtccggcaa ttagcagaag cagaggaaga aaaagaaaat tatcacctat
    17821 tgactaagct ggggattcca caaagaagga tcagacggat gatcttcgag caaaacgcac
    17881 ttaccttctt tccacccatg atattaggga tcactcacgc agtatttgca atcaatgtct
    17941 tcactcagta tataaaaact gccgattact ggttggctta tcttgtgagt ggcttattga
    18001 tccttatcta tcttttgctt tatgtcatta cgagtcagtt atattgtcgg attatcgagg
    18061 aatgatatac ggaataagac aagataataa atagaactaa at
//
LOCUS       DANKHL010000042        17993 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000042 DANKHL010000000
VERSION     DANKHL010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.044"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            214..498
                     /locus_tag="PW101_000995"
     CDS             214..498
                     /locus_tag="PW101_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HDL2513412.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(815..1198)
                     /locus_tag="PW101_000996"
     CDS             complement(815..1198)
                     /locus_tag="PW101_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HDL2513413.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(1200..1784)
                     /locus_tag="PW101_000997"
     CDS             complement(1200..1784)
                     /locus_tag="PW101_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDL2513414.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="PW101_000998"
     CDS             complement(1831..3306)
                     /locus_tag="PW101_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDL2513415.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="PW101_000999"
     CDS             3487..3798
                     /locus_tag="PW101_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513416.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="PW101_001000"
     CDS             complement(3913..6249)
                     /locus_tag="PW101_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2513417.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="PW101_001001"
     CDS             6391..7059
                     /locus_tag="PW101_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2513418.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="PW101_001002"
     CDS             7062..8105
                     /locus_tag="PW101_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2513419.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="PW101_001003"
     CDS             complement(8342..9223)
                     /locus_tag="PW101_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2513420.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="PW101_001004"
     CDS             9382..10110
                     /locus_tag="PW101_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDL2513421.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="PW101_001005"
     CDS             10266..10583
                     /locus_tag="PW101_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2513422.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="PW101_001006"
     CDS             10576..11184
                     /locus_tag="PW101_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDL2513423.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11239..12030
                     /locus_tag="PW101_001007"
     CDS             11239..12030
                     /locus_tag="PW101_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDL2513424.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12168..12656
                     /locus_tag="PW101_001008"
     CDS             12168..12656
                     /locus_tag="PW101_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDL2513425.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="PW101_001009"
     CDS             12707..13579
                     /locus_tag="PW101_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL2513426.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="PW101_001010"
     CDS             13755..14117
                     /locus_tag="PW101_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2513427.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="PW101_001011"
     CDS             14126..14878
                     /locus_tag="PW101_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2513428.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="PW101_001012"
     CDS             14892..16694
                     /locus_tag="PW101_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2513429.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..>17993
                     /locus_tag="PW101_001013"
     CDS             16971..>17993
                     /locus_tag="PW101_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL2513430.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYK"
BASE COUNT         5637 a         3126 c         3720 g         5510 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttattt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca ctacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatac tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaa
//
LOCUS       DANKHL010000043        17579 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000043 DANKHL010000000
VERSION     DANKHL010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.045"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..184)
                     /locus_tag="PW101_001014"
     CDS             complement(<1..184)
                     /locus_tag="PW101_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513431.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLV"
     gene            complement(172..1524)
                     /locus_tag="PW101_001015"
                     /pseudo
     CDS             complement(172..1524)
                     /locus_tag="PW101_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(1521..2453)
                     /locus_tag="PW101_001016"
     CDS             complement(1521..2453)
                     /locus_tag="PW101_001016"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL2513432.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2450..3226)
                     /locus_tag="PW101_001017"
     CDS             complement(2450..3226)
                     /locus_tag="PW101_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDL2513433.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3280..6465)
                     /gene="carB"
                     /locus_tag="PW101_001018"
     CDS             complement(3280..6465)
                     /gene="carB"
                     /locus_tag="PW101_001018"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDL2513434.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6470..7546)
                     /gene="carA"
                     /locus_tag="PW101_001019"
     CDS             complement(6470..7546)
                     /gene="carA"
                     /locus_tag="PW101_001019"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDL2513435.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7551..8831)
                     /locus_tag="PW101_001020"
     CDS             complement(7551..8831)
                     /locus_tag="PW101_001020"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDL2513436.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8850..9776)
                     /locus_tag="PW101_001021"
     CDS             complement(8850..9776)
                     /locus_tag="PW101_001021"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDL2513437.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9789..11072)
                     /locus_tag="PW101_001022"
     CDS             complement(9789..11072)
                     /locus_tag="PW101_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDL2513438.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11086..11625)
                     /gene="pyrR"
                     /locus_tag="PW101_001023"
     CDS             complement(11086..11625)
                     /gene="pyrR"
                     /locus_tag="PW101_001023"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDL2513439.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11860..12771)
                     /locus_tag="PW101_001024"
     CDS             complement(11860..12771)
                     /locus_tag="PW101_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2513440.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12768..13232)
                     /locus_tag="PW101_001025"
     CDS             complement(12768..13232)
                     /locus_tag="PW101_001025"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDL2513441.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(13237..13935)
                     /locus_tag="PW101_001026"
     CDS             complement(13237..13935)
                     /locus_tag="PW101_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2513442.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(13922..14308)
                     /locus_tag="PW101_001027"
     CDS             complement(13922..14308)
                     /locus_tag="PW101_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513443.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(14455..16125)
                     /locus_tag="PW101_001028"
     CDS             complement(14455..16125)
                     /locus_tag="PW101_001028"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDL2513444.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16308..16541
                     /locus_tag="PW101_001029"
     CDS             16308..16541
                     /locus_tag="PW101_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HDL2513445.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(16640..17071)
                     /locus_tag="PW101_001030"
     CDS             complement(16640..17071)
                     /locus_tag="PW101_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HDL2513446.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            17150..17563
                     /locus_tag="PW101_001031"
     CDS             17150..17563
                     /locus_tag="PW101_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDL2513447.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
BASE COUNT         4860 a         3972 c         3122 g         5625 t
ORIGIN      
        1 taaccagtct tagaaaaatg aattttaaaa aaatcaaaaa ctgatcacga tagaagtttc
       61 ttttcatacc tgatgaaaag atgcttctcg caatcagttt ttggattttt gctattccaa
      121 ttgatgattc tggagacaca aatcggattt ctctatcttc atcaagcgcg cttaaatctg
      181 ccaattttca ggatcttttt tccattcttt tagcagagtt aattcttgct catcgatata
      241 acgatcttct aaagctgctt caatcaatac agaataattg gtcaacgtca taagagggat
      301 ttcggctttt tcgaaatttg cttttccttt tggcaactca tacgtaaaga tagccgcaac
      361 accaaggata tccgcacctt ctctttttgc tgcttcagct gcttccaaga cacttccgcc
      421 tgtagaaatc aaatcctcga tcacaaccat cttctgacct tcaaatatac gtccttcgat
      481 ttggttacct ttcccatgat ctttcgcctt acttcgaata tagaccattg gcaaatccag
      541 tatttccgct acccatgcgg catgagggat tcctgctgtt gcagttccag caatcacttg
      601 tacttcaggg taagcttctt tgattttgct tgctaatcct tttgcgatct cttttcggac
      661 ttttggatag ctcatcgtga ttcggttatc acagtaaatt gggcttttga ttccacttgc
      721 ccatgtaaaa ggattacttg gatttaaaaa tactgcttca atttctaata agtcttttgc
      781 gatactttgt tcaacattca ttttaggtta gctccattct gtttgaatcg tttgatatgc
      841 tttataagga ttagttgact gagtgattgg tcgacctata acgataaaag ttgagccgat
      901 cttttttgcc tgagaaggtg tgactactct tgtctgatct cctgcgactg ttccttctgg
      961 tcttataccc ggagttaagc aaacaaattc gtctcctgcc atttctttga tcgcttctac
     1021 ttcccaagca gaagaaacca caccatctaa tcctgctttt ttggcacagc tagcataatg
     1081 gatcacactt ttttctaaag aaacttcgat tagttgatcg gcatgcattt cttcttcact
     1141 tgtagaagta agctgagtga tagctagaag tagcggacgt tttccacctt ctggagtccc
     1201 ctcttctaat cctcgcattg ccgcctccat catacgaata ccgccagcgg catgaacaca
     1261 ggtgagatca acaccaagct ttgccaatcc ccgcattgct ttttcgaccg tattcggaat
     1321 atcgtgaagt ttcagatcta aaaatacatt atgaccagcc tcttttagat aacgtacgat
     1381 ttcaggtcct tcttggtaga acaattccat tccgattttc acaaacaatt tttcttcttt
     1441 cgggaaaaat cgaagaaagt cttctacctc ttgcctagtt gaaaaatcta atgcaataat
     1501 tggtcgttgg tctagttgat tcatcgttca cgttcctctc tcacttcttt gattaatctt
     1561 tccagcgatt cgatgcctag ttcgctcatt cgcacaggga gctctttaat tagtttagga
     1621 cagatatacg gatcggtgaa attcgcggtc cctatagcta cagcacttgc tccagccata
     1681 aacatctcaa gaacatcatc cactgtctgc acgccgccca tcccaatgat tggaaggctg
     1741 gatatacttg ctacttggtg aatcaaacga atagcgacag gcttgattgc tggtccggag
     1801 agtccacctg tttgattggc taaaatcggt tttcttgtct taaggtcaat ccgcatacca
     1861 agaagagtat tgatcatcgt aaacccatct gcccctccag cttcgattgc ctgagcgatt
     1921 ggaacgatgt ctgttacatt tggggacaat ttgacataga caggaacaga agaaactttc
     1981 tttactgctt gtgttaattg aaaagcaaca tcagaatctg ttccaaaagc aattccgcca
     2041 tgtttgacat ttgggcaaga aatgttcagc tcgattgctt tcacatttgg tgcttcgcca
     2101 attttgctac aaacttctac atagtcttct tcacaagcac cagcaacatt tgcgatgatg
     2161 ggtaactcat tgtactcctc taattctgga agaagtttat gcataacaac gtctagacca
     2221 ggattttgca aaccaattgc attcaacatg ccagatggtg tttccgccac tcttggcgtg
     2281 ggattgccaa agcgggcatt tggagtcgtt gccttgacca tgatcgatcc aagctgattc
     2341 aaatcatagt attttccgta ttctttgcca aaaccgaaac agccgcttgc cggcatgata
     2401 ggatttttta attctaggcc aggaagtttg accgctaaag gattctcatt catataatta
     2461 cctctcctat tttaaaaatc ggtccttcgt cacaaacttt cacacttttt gttcctgttt
     2521 catcttccgg cgtatgacaa acgcaagcat agcaagcccc tatcccacat gccatgcggg
     2581 attctaatga taactggaca ttttgatgcg aagaaaacag ctgttccact gtcttcaata
     2641 acccgtttga accacatgca aaaacagctg taggttccgt cttcgttcct aacaataaat
     2701 taccgacatt tccgtgcata ccgtatgttc catcgtcagt agcgattctt gtttctccta
     2761 gacgattaaa ttctttttca taataaacga cttcatgtgt ggcaaaacct aaaaaatgcg
     2821 ttggcttaat tcctttttcg actaactgtt ttgataactc atatagagga gggacaccga
     2881 ttcctccacc gattataaat acttcgtctc ctgtatgaag attggacaaa tcaaaaccat
     2941 tgcctaaagg acccatgaca tctaaatggt ctccggcaga aagagtagag aagactctag
     3001 tcccctcacc ttccacccga taaatgatac ggcaagtatt tgtttcgtgg tcgatatgat
     3061 tcaagctaat cggtcggcgt aatagcaagt ctgcacgcgg cacgagaata tgcaagaact
     3121 gtcctggttg ctgcatttct tttactagat cacctttcaa tgtcatttca aaaatctttg
     3181 gtgccagctg tttttgctga acgatttcca tcatttcttg tttcatctgt cccctcctgc
     3241 aacggttttt cgtctttttg tcttaacatg tgctcgccgt taaatagggt tagttgaaaa
     3301 agcacgggat tccatcactc gaacgatcgc atctgctgtg tctaaagaag taaacaatgg
     3361 aataccatgt tcaactgctt ctcttcggat gatgaaaccg tctttagaag catttccccg
     3421 atctttatca atcgtattga caaccacttg tgttttacca tctcttatta agtcgactac
     3481 atttttttct gctgcttcct cagaaatttt cgctactgtt tcaacgacca gaccattttt
     3541 ttcgaaataa gcagctgtat gtttcgttcc tatcagacta tagccaattt ctgtaaaacg
     3601 tttagctaat tctagtgctt cttcttttgt ttcgtccgca atcgtaaata atacggctcc
     3661 atattcaggc aagtgtaatc cagaagcttc gaatgccttg tacaaagctt tgtctaattg
     3721 tctgtcagat cccattactt ctccagtcga tttcatttca ggccctaagt aagtatcaac
     3781 tttctgcaat ttcgtaaaag aaaagacagg tgctttaaca tgaacttctt ttgtttctgg
     3841 ataaagtccg ttttcataac caagtgtctt tagtttctct cccaaaatag ctttcgtcgc
     3901 aatttgtgcc attggaattc ctgtcacctt acttaagaat ggaacagtcc gacttgctcg
     3961 aggattgact tcgatcacat aaacctcatt ttcatggatg acaaactgga tattcatcat
     4021 cccgacacaa ttcaaaccta atgctaagcg gatcgtgtaa tcttcgatcg ttttcttgat
     4081 cgattcagat aatgtttgag gtggatacac agccattgaa tcccctgaat ggacaccagc
     4141 acgctcaata tgctccatga tccctgggat gagtactgtt tcaccgtcac aaatcgcatc
     4201 cacttcgcat tcaccaccaa gcaagtaacg gtccactagt actggatgtt ctggagatgc
     4261 tttcacagca tgttccatgt aatcttctaa atctcgctgg ttttcaacga tttccatcgc
     4321 acgtccgcct aatacatagc ttggacgaac aagaactgga taaccaattc tcgaagcgat
     4381 aaccacggct tcttctttac ttgtagcagt atcccctaaa ggttgaggaa tatccaactc
     4441 tttcagcgct tgctcgaata aatcacgatt ttccgcacga tccaagtctt cgatcgttgt
     4501 ccctaagatt tttacaccag cagcagtcaa aggttcggct aagttgattg ctgtctgacc
     4561 accaaattga acgattactc ctatcggctg ttctagctcg atcacattca tcacatcttc
     4621 gaaggtcaat ggctcaaaat ataatttatc cgaaatagaa aagtcagtcg aaactgtttc
     4681 aggattgctg ttcatgatga ttgcttcgta tcctgcctgc tggattgctt tgacagaatg
     4741 cacagtcgca tagtcaaatt caaccccttg tccgatacgg ataggaccag atcccagtac
     4801 tagaattgag ggcttttctg atcgtgaact ttcattttca tattcatagg aactataaaa
     4861 ataaggtgtt tttgattcga attccgctgc acaagtgtca accatcttat atacaggaat
     4921 caaatcattt tcttttctca tctggcgaac ggcttcttcg tttatttccc acagctccgc
     4981 cacttttttg tctgtaaaac cgttttgttt cgctgttttc aatatcttgt catccaacgg
     5041 ctgctgtttc aactgctgtt cgatttctac gatatgcaac agtttatcta aaaagaataa
     5101 atcgattttt gtcaaatccg ctaattcttc tatggtgtac cctcttctaa tagcttcagc
     5161 tacataaaac aggcgatcat cttgtgctcg aaccactttt tggattaggt catcgtccgt
     5221 gatgtctgcc aactcaggaa gttcgatatg gtgtgtaccg atttctaaag aacgcacagc
     5281 tttcaataaa gactcttcga tattccgacc gatagccatc acttcaccag ttgctttcat
     5341 ctgcgtgcct aaaagccgtt ctccgttttc aaacttatca aacggccagc gaggaatttt
     5401 agctacaaca taatctaatg caggttcaaa ttctgcaaaa gtcgttcctg taacaggatt
     5461 tttcatttcg tctaatgtta atccaagcgc tattttagca gctagcttag cgatcggata
     5521 gccagtcgcc ttacttgcta acgcagatga acgagaaaca cgcggattga cttcaatcac
     5581 ataataatta aaactatgtg gatcaagagc gagttggaca ttacagcctc cctcgatctt
     5641 aagtgctcga ataatcgaaa gagaagcatc acgaagcatt tgatattcat ggtcagacaa
     5701 tgtttgacta ggggcaaata cgatagaatc acctgtatgg atacccaccg ggtcaaaatt
     5761 ttccatattg cagacaacga ttgcattatc agccgaatct ctcatcactt catattcaat
     5821 ttctttaaaa cctgcaatac ttcgttcaat caaacattgg gtagcggggg aaagacttaa
     5881 tccattttct gatacgatac gtaattcttc ttcattttca cacatacctc caccagttcc
     5941 gcctaaagta aaggctggac gaacgatgac aggatagccg attttatttg caaaatctac
     6001 agcttcttct actgtattga cgatttcaga ctcggggatt ggctgattca attctttcat
     6061 caactgtttg aatagatcac gatcttctgc ttgatcgatc gcagaaagct ttgttcctag
     6121 aagttctatg ttcaattctt ctaaaatacc tgatgctgcc agctccattg ccatattgag
     6181 gcctgtctgt ccgcctaaag ttggaaggag agcatctgga cgctcttttc gaagaatcct
     6241 tgatacaaat tcaagtgtga tcggttcgat atatacttga tctgccactt ctttatccgt
     6301 catgatcgtc gcaggattag aatttactaa aaccacttca tatccttctt cttttaaggc
     6361 aaggcaggct tgtgttcctg cgtagtcaaa ttctgcggct tgaccaatga tgatcggacc
     6421 agatccaatc accataattt tcttgatatc tgtacgtttc ggcatctgtt tactgctcct
     6481 ttcctgcgtc catcatttcc ataaattcat caaataaatg tagtgcatca tgcggaccag
     6541 gggcagcatc tggatgaaac tgcacactga aagcgggata atctctgtgt ttgactcctt
     6601 ctactgttcc gtcatttact tctacatgag ttacgatgag tttttctgga tcaatcgtgg
     6661 cttcatcgac tgcatagcca tgattttgag aagtaaaatc aattcttccg gtcgctactt
     6721 ctcgtaccgg gtgatttaat cctcgatgtc cgaatttcat tttataggta tctgcaccgt
     6781 tggctaaagc aaataactgg tgaccgagac aaataccaaa aatcggtact tttccttgga
     6841 tttttttgat catttcgatt gcttctggca catcttttgg atcaccaggt ccattcgtca
     6901 gcatgactcc gtctggtgat agcgctaaaa tcgtctcggc atctgtgtta tagggaagta
     6961 ctgtgaggtt acagtttctt ttactcaatt ctcgtaaaat actatgtttc aagccgaagt
     7021 caatgacaat gactttccga cctgttcctg gacttggata aggtttgtta gttgagactt
     7081 gctgcacttg attggttgct agaacggttg ctttcagctg atcaaatgca tgttcgaagg
     7141 aatctccagc atcaactata ctagctttca tcgttccgac ttcacggatt ttgcgagtaa
     7201 gggcgcgcgt atcgatacct gatattccag gaattccttt ttgtttcaaa aatgaatcta
     7261 gcgtcatttg gtttcgccag tttgaagcta cacgtgcatg ttcttttact actactcctt
     7321 tgcatgttgg agaaatcgat tcataatcat ctctatttac cccataatta cccaccatag
     7381 gataggtaaa tgtaatgatt tgaccgttaa aactttgatc agtgatagtt tcttgataac
     7441 cagtcatact tgttgtaaaa acgatttctc cgaagacact agcatctgca ccaaaagcct
     7501 ttccttcgaa aactgttcca tcttctaata taagcaaacg ttccatactt ttattctccc
     7561 ttctgccaca caagttttcc gttgacaaaa gtcatttgtg tttcgccttt tactttccag
     7621 ccgataaaag gtgtattctc cccttttgat aaaaagtctt ctttatcgat cgtgcaagtt
     7681 tgtgtaatat caaaaactgc tacatcagcg ggggcaccaa ccgttagcgt tcctgcattc
     7741 aaaccaaaaa tctcagccgg tttaactgcc aaccaattga tcacttgttc caacgtgaat
     7801 cgaccagttt ctacaaaatg agtatagatc agttgaaacg ctgtttcgct tcctacgata
     7861 ccaaatggtg atttcatgaa actttggctt ttttcttcta atccatgggg ggcatggtct
     7921 gtagcgatgc aatcgatcgt accgtcaagc aagccttcga tcaaagcttc tctgtcctcc
     7981 cgtcctctta gcggcgggtt cattttccaa aagccgaaat cttcgggaat atcttcgtcg
     8041 atcaaaataa gatgatgggg tgaaacttct gctgttacgt ggattcctgc tttttttgcg
     8101 tctctgatta cacggacact ttctttagta gaaacatggc aaacatggta atgaacacca
     8161 gttgcttcag ctagcaacag atctctagca atttgagaag actcggttac gcttaagatc
     8221 ccgggaagac caagttcttc tgccttcttg ccagcgtgca taacgccgcc aaataataaa
     8281 gattcatctt ctgtatgggc tactagggct ttattgtttt ttgcagcttc tttcatagcc
     8341 agatacatcg tcccagctgt ttggacacct acaccgtcat ttgtgaaagc aaaagcacct
     8401 tcttcaatca gtgcttcttg atcaactaat ttctcacttc gcagattttc tgtgattggc
     8461 gcatactgca ataccttcac aactgcatct tttctaatga tgtcatatac ttgtctcagc
     8521 ttttctgccg tatcaggtac aggatttaaa ttcggcatcg cgcatacagt agtgaaaccg
     8581 ccacgagcag ctgagcgagt cccagcttcg atcgtctcct tgtatgtaaa tcctggctct
     8641 ctcaaatgaa catggacgtc cactaaacca ggtgtgatca attgtccctt tgcatcaaac
     8701 acttcatcaa attcggcttc tgagaatcct gtaccgattg ctttgattcg cccatcttct
     8761 atccaaattt ctgctggtgt cgtcatattt tttcgtgtat tgatctgacc gtttttaatg
     8821 agtgttttca ttttttcatc ctcctataat tacgcttttc catttagcac tgcttctaag
     8881 attgccattc tgacgaatac tccattgctc atttgctgaa cgatccgcga ctgtaatcct
     8941 tccactaatg aatcagctaa ttctacatca cggttaactg gtgctgggtg cataatgatt
     9001 gcatgttttt gcaacttctt cgcacgctca actgttaacc catgttcctg atggtagcct
     9061 tcttttgaga aactttcttt tccatcatgg cgttcgtgtt gcacccgaag catcatcatg
     9121 acatcaactt gatctagtag ttcatccaat ggcatataat gtccgtacac ttcgaattca
     9181 tcgtcgtacc attctctagg acctgagaag aatacttgtg cgcctagacg ttttaacatc
     9241 tgcatattgg atttagctac gcgagagtga gtgatatcac ctacgatggc aactttcaga
     9301 ccttcaaaat gaccaaattc ttcataaatg gtcatcaaat caagcaagca ttgggttgga
     9361 tgctgtccgc ttccgtctcc tccattgata atcgaacact ggattgtttt actttgtatc
     9421 agttcgtcat aatagttctc atcaccatga cggataaccg ctacgtctac gcctaatgct
     9481 gacatcgtca gtactgtatc gtatagtgtt tccccttttt ggacagagct tgtgcttgct
     9541 tcaaattcaa tcacttctag ccctagtttt ttctccgcta catcgaagct tttatgtgtg
     9601 cgagtactat tttcgaaaaa gaggtttgta gcaaagtact ggcgcttttg cggagtccaa
     9661 ttagcacctt gcttgaattc ctgccctctg cggatcaagc ccataacttc ttgatcagta
     9721 agtgcctcaa cggtcaataa atgctttaag ctgatacgtt ctgatttgat gatcataatg
     9781 tttcctcctt agttttcttc gcttcgtgaa gtttctggta agataagatt taaaatgatg
     9841 ccaagtacag ttgctaatgc cattgccgac aatgtaaatg tgcctacttc aaagacgagt
     9901 ccgccaattc ctactactaa gataactgaa gcaatcaaca gatttttctt tttatcaaaa
     9961 tcgattttgt tctcgattaa aatcttcatt ccacttgcgg caattactcc aaataagata
    10021 aaactgattc ctgagatgac tggtccaggg atacttaaaa tcaaagcgct taattttcct
    10081 acgaatccta aaatgactgc aaatactgca gctccaccga tcacaaacac gctatggaca
    10141 cgtgtgatag ccagaacacc aatgttttca ccgtaacttg ttacaggagg acctccgaca
    10201 aaggctgcca cgatttgtgc taatccatca cctgtcaacg tcttatgaag acccggttct
    10261 tcaaagaaat tacgtttagt tagtttattc aataccataa gatggccgat atgctctgtc
    10321 atcgtcacaa aagcgatggg tgccattgtt gtgattgctc caagatatag ctttggttca
    10381 tattgtacaa agggaatctc aaagttagga acagcaaacc aggcagcgtc tacgattggc
    10441 ttagtatcca cgattccaaa cagcaaggct acgatgtatc cgctaataat acctagtaaa
    10501 atcggaatca atccgagaaa acctcttagc cacatgttga agaaaattgt taatcctaat
    10561 gtgatcagcg ctacagcaat atacttgaaa tcatacgtgc cctgattata catagcattt
    10621 gttgctgcat ttgccgcaag tcccaacccg ataaccatga tgactggacc gacgacgatt
    10681 ggaggcaaga ttttatccag ccatgctgaa ccgatctttt tgatgatcag cgcaacaatc
    10741 aaatagacta atccagttgt gactgctcct tgtgcaatcg ctggataacc atctgttttc
    10801 atcaaaagct gcatcgctgc aatgaaagcg aagctgcttc ctaagtacgc aggtatcttt
    10861 cctttagtag taaataagta caccaatgta cctagtcctg aactgactag cgcaatacct
    10921 ggatcgatac ctactaagat ggggactaac acggttgctc cgaacatcgt gaacaagtgt
    10981 tgtaaactta atcctaccca atgtcctggg gttgggcgat cgtggatatc taacaccacg
    11041 tcatcattat gaaatccttt tttctccgac atgtttctct ctcctctaat cttcctcttt
    11101 caaaatcatg atgcgatctt gtccatcaag ctcttgcatc tctactaaaa tctcttccgc
    11161 ccgagatgtt ggaatgttct tcccaacata atctgcacgg attggcaatt ctcgatgccc
    11221 acgatcgacc aggactgcta atgaaatttt tcttggtctg ccaaaatcca ttactgcgtc
    11281 cattgctgct cgaatcgttc gtcctgtata caaaacatca tcaatcaaaa taacttcttt
    11341 tccttctaag gaaactggga tgtcagaaga atgaagttcc ggttcttctg gattttcttt
    11401 tttgtcatct cggtataaag tgatatccag ttctcctaca ggaatatcga tatcttctag
    11461 ctgcttcagg cgttcggcaa ttcttgatgc aatgtagatt cctcgtgttt tgatacctac
    11521 caatacgata tcttggatac tatggtttct ctcgataatc tcataagtaa ttcgagtcaa
    11581 tgctcgcttc atggttacct gatctactac ttcttttgct tgcattttca ttccccctac
    11641 ttttttgcat aaaaaaactc ctgccctaag aggacaggag gaatgtttag actgagcgta
    11701 tactgataca ctgatagcta tactatgtca atgagcaatc tacttatggc gcaccatacg
    11761 tatccttgct cccacccttc ttagcctcac gggactaaac ttaaaggaat tctattaagt
    11821 tttatgcttt cgttgttaat tactataccg ttatctaaat taaaatgcaa tgttttttct
    11881 taatttttct aaagtttttt caaaaacttc tggaagaggt gcttcaaaga tcatcttttc
    11941 gtttgtagtc gggtgcgtaa aacctaacat ttgcgcatgg agaaactgac cattgccttt
    12001 caaagttttc ttcggtccgt atactggatc tccagctaac ggataaccga tatacttcat
    12061 atgcacacgg atctggtgtg ttcttccggt ttctagtttt aattcaacca gagtaaatcc
    12121 ttcgaaacgt tccaatacag taaaatgtgt gacagccgac ttcccatctt ctcttatcgc
    12181 ctgcattttc cggttttcct tcgaacggcc gattggcgca ttgatggttc ctttttcgtg
    12241 aggaatctct ccatggacta aagcaacata tttgcgcaaa gaggtcttat cttttaattg
    12301 ctgagctaat gattcgtgtg cctggtcatt tttggcaacc attagcagtc cggaagtatc
    12361 tttgtcgatt cggtgaacaa tacctggacg aataacgccg ttgatcgtag aaagatcctt
    12421 gatatggtac atcagcgcat tgaccattgt gccatgcgta tgacctgctg aaggatgaac
    12481 gaccattcct tgaggtttgt ttatcaccgc tacatcttca tcttcgtaaa cgatcgtcaa
    12541 cgggatattt tctgggacca catctaatgt ttctggttcg gggatcaaga cagtaatctc
    12601 gtctccttgt tttactttgt aatttgcttt gactggctgt ccattgacag aaaccgctcc
    12661 atttttcagc cacagctgaa tttgcgaacg actgtaatcg gtgaataaat tagtcaatac
    12721 tttatcgatc ctaccagatt catggtgaat cgtcgtttta agttctgtca tttttttctt
    12781 tgatcctttt catctaaaat aatataaatt aacatacaaa tcactccaca tacaagtgtc
    12841 acatctgcta cgttaaaaat cggaaagctc ataaaatctg tctggaacat atctactaca
    12901 tagccaagac gaatacggtc gataaaattg ccaatcgcac cagctagaat caaactcaat
    12961 ccaatcatga accagcgatt acctttttca cgatttttaa tcatcaatgt gagaatcaca
    13021 acgactgcaa tgagtgtcac tatagtaaag aaccacatct tcccttctag catactccat
    13081 gcggctccag tgtttcgaat atgattcagt gacaaaatac ctggtataag atttttcgtc
    13141 tctccaagcg ctagattatc agtgatccac catttgacca actgatccac tcctactagc
    13201 aaagcgctga atatccaata aataattaac aaaaaatcaa tccttttcta tttgtttata
    13261 aatatcacga ggtcgtacga tcccgagcag attaccatcg ggattcccat ctttagtgat
    13321 aagtacggct tccaaagtcc cttgctcacg aaacatcgct tcaacctcgt aaatatgtgt
    13381 tttagctgga acaaaatgat aatttgtgta ctttccgttt tggatgatca aatccaacgc
    13441 tcgtttattt gttaaatcaa ccggaccttt ttggctttct tttgctagcc aaaacccgat
    13501 attacgtaaa gtaatcaatg ctacaaattt tcctttattg tataacggaa attgtgaata
    13561 ccttttttct gctactatct ttaacagttc aagtaacgga agatcttgtt caaaacctgt
    13621 tacacgtttt gcaaatcgag gcagcacggt ttcaggctga atcagttctt gttcgatcag
    13681 ctggattcgc ttgactgtcc aatcatttgg ctctgcaatg acaaaatcga ctgcgatccg
    13741 atcatggacg atcgcattcc ttaactgagc aagctgtaaa agatcatctt cgtattttct
    13801 gatcattaat tgttttcttt gagctaggcg tctgacaagt tcggtaaatc ccatatttcg
    13861 tgcatttccc atctcgtctc tcatccattt ttcgattcga ttgaaactga ttaaaaaaag
    13921 atcagagttt gccataattt tcctccttga tcaaatatgc ccggtttttt atatagaaaa
    13981 agaacgcaat caacacaata actgcaaaaa aactgaaaaa taaattttgt aggaaaacgg
    14041 cccccaaccc agttgctaga aataaaaacg gatagatttg aagaatcaag taattcattg
    14101 tgatcacata agcaggcttt gcttctttca ctgtataaac ggggcttttt agccaataaa
    14161 aggtgacctg catcgtaaag ggcacttcca gatcaagcgt tatcttttgt tcatgactga
    14221 gaacactgcg tttttcattg ttgattctta gctgaatcga gctacccata ccgtaaaaac
    14281 cggtttttct tataaatgtc gttttcatca taccctccta ttatgcttta gtatagctca
    14341 aaatacaata aagaaaaaga atgatcctag tgtaaacaaa attaattccc tatcgtctaa
    14401 aaaaagaagc tgtagtcaaa atacagctcc ttcgatggta gattataatc aagcttaaaa
    14461 caatcccaac acttctccgt catttgtcac gtccatattc aatgcagcag gttttttagg
    14521 caaaccaggc atcgtcataa catctccagt caaggctact aaaaatcctg cccctaattt
    14581 tgggatgatt tcgcggatcg tgatggtgaa atcttccggt gcacctagca gtgaaggatc
    14641 atcagaaaat gaatactgtg tttttgccat acaaatcggc agtttgtccc atccattctt
    14701 cgtaaactct tctatttgtt tttgcgcttt tttgctgaaa acaacaccat ttcctccgta
    14761 aattttcttg acgattgttt ctgtcttttc ttgtatcgtt tcttctaggc ggtataatgg
    14821 cttgtaatta gcttcttttc tatcaatcaa tcgtactaca gcttttgcta aatcaacgcc
    14881 gccttctgct ccatctgccc atacactcgc tctcttggca agaatcccct gattttcaca
    14941 caagtgctcc aacagtgtga gttcattgtc tgtatctgtt acgaattcat tgattgcgac
    15001 gatcacaggt agctgatatt gttccatatt acgtatatgg cgctttaaat ttgcaaaacc
    15061 tactttcaaa gcatcaagat tctcattttt aagttcatct tttttcattc cgccatgcat
    15121 tttcaatgca cggacagtgg cgacaatcac cacagcatcc ggcgcttttt tcaagttagg
    15181 tactttgata tctaaaaatt tctctccgcc aagatctgca ccaaatcctg cctctgtgac
    15241 aacgtaatct gctaatctca atgctgtctt ggtagctaaa atactattgc agccatgagc
    15301 gatattggca aacggtcccc cgtggacaaa agctggtgtt ccgtaaattg tttgtacaag
    15361 attcggcttg attgcatctt tcagcagtaa tgccagagcg ccttctgctt tcaagtctcc
    15421 agcagtcaca ggctgacggt caaatgtata gccgacaacg atccttgcaa gacgagcctt
    15481 taaatcttca aggtctgccg caagacacaa gatagccatg atttcgctgg caactgtgat
    15541 atcaaaccca tcttctcttg gaacaccttg gattggccca cccaagccaa caattacttt
    15601 acgcagttca cgatcattga gatcgactac ccgtttccaa atcactcggc gggcatcgat
    15661 gttcaattca tttccttgat gaatatgatt atctaaaaga gcagataatg cattattagc
    15721 tgttgtgata gcatgcatgt caccagtaaa atgcaagttg atatcttcca taggcaatac
    15781 ctgtgcatat cctccgcctg cagcaccacc tttgattccc atcacaggac caagtgaggg
    15841 ttctcgcaat gcaataacag attttttatt gattttattg agcgcgtctc ctaaccctac
    15901 agtgatcgtt gactttcctt cgccagctgg tgttggattg atggaagtaa ctaagatcaa
    15961 atgcccctca gcgttatcct ttgatcgatt gattccagaa aaattgattt tcgccttata
    16021 tttcccatac agttccaaat cgtcttcctt aagatctaat ttactagcca cttcaacaat
    16081 cggttctagc tctgtttctt gtgaaatttg cagatcattc ttcatttgat tacccctttt
    16141 cacgaacttt attcaacaaa atatcttctt tgttaagata tgccaattat accttttttt
    16201 attcagaaaa aaaacataaa attacaggtt tttaatttat ttgacgtact tataaatact
    16261 ctttataatg aaggctatga tcagacttta gaaaggtcgt gagtgctttg accacaggaa
    16321 ttgtaaaatg gtttgacaac aaaaaaggat acggctttat cagttatgat gacacagaag
    16381 aaatttttgt acattttact gcgattgaag aagagggatt caaaactttg gaagaaaatc
    16441 aagtggtcga atttgagatc atcgaaggga atcgaggaac acaagctgcc catgtcaaga
    16501 aaggcaacgt tcagactacc gatactagta tgcatgaata gataaacaaa tagagtgaga
    16561 cgctaaacgg gatgaaccgt acgtctcact cttttctttt cctgtaaaaa ttcgtttttt
    16621 taggttctga tttttaaaat tagctttttc tttttttcgt ataaggataa ggaattgatt
    16681 ctgatttggg tccgtttttt tcaaaatgat caaaaaggac tacttgatct ttttttcgca
    16741 tcatgaaaac agatggggct ttctcatctt gcaacatcat ttgcacccca gtattagttg
    16801 gcgtcaaaga tcgaatctgt ccgacagata tttctcggtc tttccaaaaa gcagcataac
    16861 gtataatcac agagtttgat tttaaaataa aagaacgccg caaacctaga aatagaaaaa
    16921 caagaaaaat acctaaaaca aaattgcttt tccaataagg acctgtgttt tctaaagaaa
    16981 gaatcagact aaaaaacaga atgatcaagg tcaatgacca atagatgatc gtatgtgcca
    17041 gttcaggctg ccatcgaata ttttttttca ttctctaacc tctcaccgat tcagtaatga
    17101 tataatctta ccataacatt agtttgaaca aaaaggagaa tctcttttta tgttaaaagt
    17161 atacattgat gcctctacaa aaggcaaccc tggtcctagt ggaggtggaa tcttgattgt
    17221 ctatcaaggt aaacaagaac agctatctat cccgctttct atagggacaa accatcaagc
    17281 agagttcgaa gtctttttaa aaactttaga atttctaaag aaaaacgact tacagaatga
    17341 aacgattttc tgtttttcag acagcaaaac actagtttct acggtcgaca aaaacgcaac
    17401 gaagaacgag ctcttccttc cctaccttca aagaatccaa gaacttctat ccgaattttc
    17461 tttattgatc ctgcaatggt tgcccgaaaa caaaaataaa ggagcagata atcttgcacg
    17521 tcaggctctc cataaacaat taaaagaaaa aaagactctt taaattaaaa actttaagg
//
LOCUS       DANKHL010000044        17534 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000044 DANKHL010000000
VERSION     DANKHL010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.046"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..198
                     /locus_tag="PW101_001032"
     CDS             <1..198
                     /locus_tag="PW101_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513448.1"
                     /translation="LINKNDKNQGFLQFYGAAYAVLGVMGILTAFFNQRFIALVFLLI
                     VIIVSATFSIRFAKKIAEPKQ"
     gene            271..750
                     /locus_tag="PW101_001033"
     CDS             271..750
                     /locus_tag="PW101_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2513449.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            973..1116
                     /locus_tag="PW101_001034"
     CDS             973..1116
                     /locus_tag="PW101_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HDL2513450.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            1694..3277
                     /locus_tag="PW101_001035"
     CDS             1694..3277
                     /locus_tag="PW101_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2513451.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(3714..4898)
                     /locus_tag="PW101_001036"
     CDS             complement(3714..4898)
                     /locus_tag="PW101_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDL2513452.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(4923..5930)
                     /locus_tag="PW101_001037"
     CDS             complement(4923..5930)
                     /locus_tag="PW101_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2513453.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(6102..6461)
                     /locus_tag="PW101_001038"
     CDS             complement(6102..6461)
                     /locus_tag="PW101_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513454.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(6433..6804)
                     /locus_tag="PW101_001039"
     CDS             complement(6433..6804)
                     /locus_tag="PW101_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HDL2513455.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(6821..7123)
                     /locus_tag="PW101_001040"
     CDS             complement(6821..7123)
                     /locus_tag="PW101_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513456.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(7140..7610)
                     /locus_tag="PW101_001041"
     CDS             complement(7140..7610)
                     /locus_tag="PW101_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDL2513457.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(7585..7863)
                     /locus_tag="PW101_001042"
     CDS             complement(7585..7863)
                     /locus_tag="PW101_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDL2513458.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(7860..8909)
                     /locus_tag="PW101_001043"
     CDS             complement(7860..8909)
                     /locus_tag="PW101_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HDL2513459.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(8866..9795)
                     /gene="tadA"
                     /locus_tag="PW101_001044"
     CDS             complement(8866..9795)
                     /gene="tadA"
                     /locus_tag="PW101_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDL2513460.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            9927..11681
                     /gene="ade"
                     /locus_tag="PW101_001045"
     CDS             9927..11681
                     /gene="ade"
                     /locus_tag="PW101_001045"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDL2513461.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(11779..12441)
                     /gene="trmB"
                     /locus_tag="PW101_001046"
     CDS             complement(11779..12441)
                     /gene="trmB"
                     /locus_tag="PW101_001046"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDL2513462.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12511..13293)
                     /locus_tag="PW101_001047"
     CDS             complement(12511..13293)
                     /locus_tag="PW101_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDL2513463.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(13497..14708)
                     /locus_tag="PW101_001048"
     CDS             complement(13497..14708)
                     /locus_tag="PW101_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2513464.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(14701..15438)
                     /locus_tag="PW101_001049"
     CDS             complement(14701..15438)
                     /locus_tag="PW101_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2513465.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            15576..16010
                     /locus_tag="PW101_001050"
     CDS             15576..16010
                     /locus_tag="PW101_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDL2513466.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            16003..16347
                     /locus_tag="PW101_001051"
     CDS             16003..16347
                     /locus_tag="PW101_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL2513467.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            16527..>17534
                     /locus_tag="PW101_001052"
     CDS             16527..>17534
                     /locus_tag="PW101_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2513468.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         5602 a         3572 c         3100 g         5260 t
ORIGIN      
        1 ttgatcaaca aaaatgataa aaatcaagga ttcctacaat tttacggagc tgcatatgct
       61 gttttaggcg taatgggtat tttaacagct tttttcaatc aacgctttat tgccttggtc
      121 tttttactga tcgtaattat tgtttctgcc accttcagta tccgatttgc aaaaaaaatt
      181 gctgaaccaa agcagtaaat aattctcttt ttccctaaag tgttctacaa taaaagagta
      241 caatacgatt gacttgagga gtgaatgctt atgttacaac aatacaaaaa aatcatggtt
      301 gccgtagatg gttctgatga agctgaatta gcttttaaaa aggcggtcaa tgtagctatc
      361 cgaaataacg gagaacttct attagcacat gtcattgaca cacgttcttt ccaaaccgtt
      421 tcatcctttg atggaatgct tgcagaacaa gctactgaaa tggcaaagca aacattggca
      481 gattatgaaa gcaatgcgaa gaaagctggc ttgaataatg taacctctgt cgtagaatat
      541 ggctcaccaa aacaaattat tgccagagaa atcccagaag ataatcaagt agatttgatt
      601 atgttaggag ccacaggatt gaatgctgta gaacgtttat ttattggttc tgtttctgaa
      661 tatgttatta gaaacgcagc ttgcgatgtg cttgttgtcc gaactgattt agaaaaccaa
      721 ttgccagcta aagaccacac caaagcttaa cacgtagacg acctgccaac tatcggcagg
      781 tttttttgca caaaaaaccg tgataaaaac aaaagacgcg gcaagcactc atcggtcttt
      841 ataatgttta aaataaaggg aaaacagtca atgagttcaa gtaaaataaa aaaaagtgaa
      901 caccagggcg attagtcgcc ctggtgtggt ttaaaacgac ctaagcatgc caaagcttat
      961 tggttcgtca atgtggaagt ttccgatgaa tctgatacga aaaaatatga agtatcgatg
     1021 gcggataccg attttatcgc caaactaaaa ttttatttcg ggttcgggta ccgcgagaag
     1081 gcaagtgcta cattcggtaa atcattcaag aattgatgaa aggctaccgc ttgccattgc
     1141 aaccacaacg atactcctga acgcgatgtt ggtgagattt ccaaagagaa gagctgttta
     1201 atgcaacggt attaagcaaa tggcaaaagg aagtcatgta gacaaagttt tttatcaaac
     1261 accatatgat ttcgcctgaa cgctggttta gctcagaata caatgatgtg gtccacgaga
     1321 cattaccctc agtcagataa aaaaagctct ctgacagaat tacacatcga taaatattaa
     1381 gtaaatgtca gcgctagttg aactttctga cactttcagg tacactggaa aagacaacta
     1441 cacgaaggag ggaaaatact atgatgcgca tgactaaaat aacaagtaat gaaaattacc
     1501 aacgatacca tatcgcattt agccatgcaa ttggttttac agttgctgtt gacgggacaa
     1561 gtttgtccaa aacagttgaa ttcacgtaaa atcaatggcc atctcaaggt atttttctca
     1621 tgataatctg agtttaaata acacaggcca tggataacca tggccttttt gagtggtctc
     1681 aaggagagag atcatgatca tacaagcaat ttctattaaa aaaacaatcg gcgggaatcc
     1741 attatttgag gatttatcct gtcaaattga agctggtgaa aaaattgcct tagtcggtat
     1801 caacggaact ggcaaatcaa cctttttaca aatcttgacc ggcagagaag gtgtcgacag
     1861 cggtgttatc agtcgcaaaa aaggcttaaa gatcgggacg gtcgaacaag aacttacagt
     1921 ttccgaagcg accgtcaacc actacttgct ccattctgcc gctgagatac aggacctcaa
     1981 acagcaaatg agtcgctacg aagcattgat gacagaacca gatacaaact tagagaagtg
     2041 tctggcccgt tacggtgagc tgcagcatcg ctttgaagaa ctaggcggtt acgtattaga
     2101 agaccggatt gcagcgatct tgcaaggctt agggattcca caccgtcagg aagcacggtt
     2161 aacagaactt agcggaggcg aacgagttaa agtcgcttta gccaaaattt tagcagccga
     2221 tgccgattta cttttactcg atgaaccgac caatcattta gatcttaaca gtattcgctg
     2281 gttggaaaac tatttacaga atacaaaaaa atcggttctg gtcgtttctc atgatcggca
     2341 atttctcgat caagtcacca caaaaacatg ggaactggaa gaaggtgcat tgattgagta
     2401 tcccggaaac tacagccgtt ttcgagtctt gaaagaagct cgcttggctg agctgacaaa
     2461 aaattacgaa ctgcagcaaa aagaagtcca gcggttgaaa gtcatgattc gtcgttttcg
     2521 tcagtgggca catgaaggag acaatgagtc cttcttcaaa aaagccaaag aactggaacg
     2581 ccgcctggcg aaactgacac tcgtcaaacc gccacctccc ccgaaaaatc ggctgcaaag
     2641 cttgtctaat ggtggaaaat ctggcaaaga agttttcatc attcaaaact tgcaccagca
     2701 gtacgctgat caagtactat tcaaagacag cagttttgca gtctatcgtg gagatcattt
     2761 agccatcatc ggcgacaatg gtgcaggaaa atcaacctta ctcaaattat tactcggtga
     2821 ggaaaagcct tacggtggca ccatcaaatt aggtagtagc ttgcaaatcg gttatttgcc
     2881 gcagcagctg cagtttgccg atcccgatag ccgtttgctg gcttacgcca tcacattgac
     2941 tggaaacgaa gaagtcgctc gacggcaatt agccaaaagt ggcttttatc aaagcgatgt
     3001 gggtaaacgg atcaaagatt tatccggtgg tgaaaaaatc cgtttggcgt tgctgaaact
     3061 ctttttagaa aaaatcaacg tcctgatttt ggatgaacca accaatcacc tcgacagcta
     3121 tgctcgagaa gaaattgaag aaatgctgca agactatcaa ggaacgctcc tagcagtcag
     3181 ccatgaccgc tactttttgc agcaacactt ccaagaagca ctcgttattg atcaggagca
     3241 gatcagccgc cagccgctcg gccaactgat tgactaggac gttttttcgc aacaaaaaac
     3301 ttgattttca tacctgtgct ttcacgattc aggcaaagta caggtggaaa atcaagtttt
     3361 tattattcga tcaatgaatg tttcggatcg gttttctagc taaacataaa gcgaagaata
     3421 aagtcgacag cccacgaggc tgatctaaaa atgttttttc tttttacata tgtaatgtcc
     3481 cttataaaga tgaatgaaaa taaaatattg ggattttcct gaaaatcaat aaataaaatg
     3541 ccagtctttt cccttttgac cacttttcca agcaaaattc cctaagaaga tcctgtaaaa
     3601 aagaagggaa attgaagtta aattaccatt ttgcggtctt ctccgcaaaa aaaacacatg
     3661 caagaattcc ctcttgcatg cgtttcatct tttatcctaa cgaatgcttt ggcttagccg
     3721 cgcagacgct ccacatcacg ggcaatcatt agttcctcat ctgttgggat cactaatact
     3781 cttacttttg attcttttgt agagatatct acttcttcac cacgcaattc attcaacgct
     3841 ggatcgattt cacaaccaaa ccaagacata cctttgatga tttcactacg tacatgtccg
     3901 tcattctcgc cgataccagc agtaaagaca attgcatcaa caccattcat cgttgtcaca
     3961 taaccgccga tgtattttcg gatgcggtcc acgaaaatgt cataagctaa acgaatatgt
     4021 tctttttcat agttcttttc aagatcacgc atatcactag acaatcctgt taggcctagc
     4081 agaccagatt tcttgttcaa aacattgatc atttcatcaa tatcaatctc taatttttcc
     4141 attaaatatg ggagaacagc tggatcgata tcacctgaac gtgtacccat tgtcacccca
     4201 gctaatggtg tgaatcccat agaagtatcc atggatttac ctccatcgac tgctgtgatc
     4261 gatacaccat ttcctaagtg gcaagtgatg atttttagtt cttcgatcgg tttgttcaat
     4321 aattttgccg cacgttccga cacataacga tgacttgtgc catggaagcc atattttctt
     4381 actttgaaat cttcataata agacaccggt aagctatata aataattttg ctttggcatt
     4441 gttgagtgga aagatgtatc gaatacagcc acactcaaaa tgtcaggtaa aatcttttta
     4501 aacgctttga tccccattaa gtttgcagga ttatgcaaag gagcaaattc tgctaattca
     4561 gcaatgcggt ccattacttt ctcatcgatc actactgaat ccccataagt ttctcctccg
     4621 tgaacaacac ggtgccctac tccggtgatt tcatcataag agcttaaaat ttgcagttca
     4681 atcaactgat ctaacagcat ttttactgct acttcgtggt tttcgatatc catgatttgt
     4741 tcgaattttt gattatcgcc gtacttgatg gtaaaaatag aatcttgtaa accgatacgt
     4801 tcgacgatcc ctttcgcaac tacggtttct tctggcattt gatataattg ccatttcaaa
     4861 cttgaactcc cagcattgat tgcaattgtt ttagacatat gactctcctt ctcgttgaat
     4921 gattacagag cagatgattt ccagttttta aactcgttga aaaattccgt caccttctct
     4981 ggttctttta atgaagcaag tttcacaagc aagacttctt ttacttgttg tgcttgtggt
     5041 ccttttttct gtaagatcaa aatacttttt tgcgattgtt tattacggaa caattcatct
     5101 ggcaattgaa tcattccttg aagataacct tcttctttaa accatttttt gatttcttca
     5161 ctttgatccg tttctaaaaa tccactcggc atcaaaaaca aaccaaaacc atccggcttc
     5221 acatatttca ttgattgctc tagcaataaa tgatgtgcat aactatggcc ttcttcggta
     5281 cttgttaaaa actcttttgc tttttcgtca ttcggataat aaccgattgg caaatcgctg
     5341 attgcaaaat cgacaggatc tattaataat tcctgcaacc cgtcttgatg aaagtattga
     5401 acatttgctt gtgtcaaatc acttgtagaa gctgcgacag ccaacaatgt atcatcaata
     5461 tccacaccaa ttccttctgt ttggtacccc gcattcgata ggtttaaaag aactgttaat
     5521 aacaagttgc ccattcctgc cgcaatatcc agaatcttaa ccggtgcttt tttatttgtg
     5581 tatagttgtt caataagaaa aacaaataaa aaaccgatac tatcaggagt gagttgatgg
     5641 ttcgcctgca aatgttctgt ctggctgccc ttcaataaaa gtaattgaga cagtctgcgc
     5701 cattcttctg gctcgaaaga aagtttcttc aactcttcat aaagagctgt gatccgttgc
     5761 gttgtctctt ttgtcggcac tccatccacc acacgtactt gataatcatc tattaaattt
     5821 tctgcatttt cgacataggc ttctaaaaag gaagttccca atgcattctg taaaagctga
     5881 atagcttcca ggttttgatt aaacccttgt tccatttttt ctggtaacat ggtgcacctc
     5941 atttctagat atattataac aattttttca cctgtttaat tctatcgaac aattgacaaa
     6001 atttcaatcg ttttcactgt tttttatttg tgaaacggca aacatttttt tattaaaaac
     6061 agtgttataa cagaaaagag ggcgttttca cgaacattcc atcattctgt actagtatct
     6121 gtatcatttt ttacttcttc tgtgaaccga aagataaacg gtgagacttc cacctctaag
     6181 aatagggact gttgtaccca ctgatatgtt acagatccag ttgtgtagta aaaaactcct
     6241 tccgaacttt cggcaagcgt agactgttcc gataaagcca tctgcttcat tatttttgct
     6301 atataataat ttttgattcg tatattaaga tccattgaca aacggtagct ttctaatgct
     6361 gccatcaata aaaacgtaca taaaagaaat aaaagcaaga cagaaaataa aatagagccc
     6421 ttataacatt ttctactgat cgttttttgt ccatatggca tatccatctt ctccattttc
     6481 aaagtgtaca taaaaagaaa ttgtgtgttc tttttccaat aattgaagct cagtgatact
     6541 tgttaacata ggttgatgtc cgccggaagt tcttctttta cgtatcattg cctgatatct
     6601 ttcgatgata tactgtttat cctcgatcgt gtagtgcagt tgctgatttt tcacagagac
     6661 attgtgactg ctcttgagtt cttcttctaa ttgtgctaaa aatattagcc actctttttg
     6721 atctttacgc tgcaaaaatt cacttacttg gatggtttgc tgaatcaaaa gattgaatgt
     6781 gagcaataga ctgctcaata tcatcagtcc aattagacat tcaatcagcg taaatgctgg
     6841 aattacttgt tttttcaata ctgatcgctt ctttctccat attgacaaac actttttcga
     6901 ttactccatt attatcgaca atttggatat cgtatgggat catttcttcc cttcgctctg
     6961 gtcttttgtg atacatccgc tgcgtcttga tatcttcata aatcattcga ataaatagca
     7021 gttcctcccc acttttcttt tctgcctgaa gcaaaaacag ctggccaaag ctcactaagt
     7081 agacacagac agaagcgatc ataagcgctg taagggactc caacaaaaca aatcctgttt
     7141 tattcaattc tttttgtata gcgaccactc cctatctgga attgatacgt aatcgtccgc
     7201 tctttttctt cccaataaaa ttgataagct tttagtgaac tttcattccc ggttctagca
     7261 gcaaaagtaa tagaagcatg gcgtttcaag gtaatctcat caggaatatc taatacttcc
     7321 caatttattt tatctggatt cggaacatga aaaatgattt gattctcttc actattccaa
     7381 taaaagcctg tttggatctg attcacaata gcgatttttt gcgtagaata gattcgtttt
     7441 tcaaattggc cgaaaaattg atagactgcc aattcattct tccatgcaga aaatgcgaga
     7501 actggaaaag aaaggatcgt tgtaagtaat aaaatcagta agagtgattc aaacaacgta
     7561 tatcctgatg ctaaaacctt accttcaagg ccgctgatag atttctgcat gttctcgttt
     7621 gatatagcct tcattcaaca attcctccac agtaggaact cttccattat tttgaagcgc
     7681 atagagctct ttttgtgtgt ctaccaactg gatgatcgct tcacgacttt cttggtctac
     7741 atgattccta taacgcgaca aattagggac gaacaacaac actaatacac tgatgatcag
     7801 taagacgatt agcatttcga tcaatgtaaa accgctataa taagatattt ttctcttttt
     7861 catggcaaaa cctcctctat gcttccgtaa acaggtaata aaacagcggc ataaactaat
     7921 aaaatcaaag ccgctacgcc caaaaaaatg attggctgaa taaaatgcat ccagtgatga
     7981 agacgtttga aaagctgttt tcttgtccat tcgctgtaaa aaatcagttc tttacctaaa
     8041 cttccctttg cctctccctg taaaacaatt ttactgaatt cttctgatag aaaacgataa
     8101 ttctggaact gctcagccaa tgacatacct gcctccaatc cttgaatcaa cttagaggca
     8161 agatgctgaa tcagactttc ttgtttcgtt tcctttaagc agactatgat ttgccttaat
     8221 tcgaaacctt catgaaaaag ctttccaatc tctagtgaca aataagccga ttgatacaag
     8281 gaatatatcg gtccaatcaa taattgtctg cttatccatt cactctgatc aacaggagac
     8341 aacttcatca gacgatacct tactagtaaa gccaatagag taaccgctag gatgaatagt
     8401 agaacatacc aatgaaagac ttgtatcagc tgaatacccc aatgctcttg catgaccatt
     8461 cctgaaccaa taagctgagg caaaacgaac aaccgcatac taaacaagat acctagcaaa
     8521 aaggtgagca atagcatcgg atatgcaagc agctttttca aatcattgcg aaatccttca
     8581 acaagccgaa actgtttggc aatttccaac aaagttgtcg gcagattccc atgacgttca
     8641 gctaactcga tctgtaccag ttgctctgct ccatatccta tttgcgaaag tacctcacta
     8701 aacgcttgcc cttcctttag ctgatgttgg atatttgcca aaataacagg tggaaacgaa
     8761 cggatcgtga gtaataaata aagggattct tgcagactaa aaccagataa aagcagttct
     8821 cccatggttt gtgcaaactg gttttgctgg cttcttgtta atcctttaga atagttctga
     8881 ctgccaaata ttttcctgag cttcttcata actttcctcc catgattttt ttactgattc
     8941 ttcctccata aattgataac tccataaaac agaaggatgg tgattggcat cttccaaaag
     9001 ttcttgataa atgacaccag ctaaacattc agatagctct tcttttttct ttgtcagttc
     9061 atggattctg accaatgtac cttcaagatt gcgtgcatgt acggttgcaa aaacccggtg
     9121 ccctgttagt gctgctctta ccgcagcatg tgcagttgat tcatctctta tctcgccaat
     9181 gattagcaag tctggacgat gccgtaatgc gagtctcaaa agtgtatcat aagtctgtgc
     9241 tattttcgga ttgatctgta gctgcagaaa aaacggttct tctatctcta caggatcttc
     9301 gatagtaatc acttgcaatt tttcttccaa agccaaccga tacattagag aagtcttgcc
     9361 tgatccgact ggtccgctga aaaggtataa tccacgtctg acggttttct gtaaaatagt
     9421 tgcaaaatct cgagaactaa agcaacgatg gggtgtttca aaagtaccgt ataatagccg
     9481 aatcactaaa ctctctcgtt gaaggtaatc tcctacagaa gacaaccgca gacgaacttc
     9541 ctgtttttct aaagaataag taaatgcacc taactgagct tttctttttt ctccgatatc
     9601 catctgtccc aaatacttaa atcttgagat cagttgctgt ccatctgccg aagagacttt
     9661 cttccagaat gttcgttttt ttccatagcg aaaatagatg taaaatactt ctgaagctgg
     9721 caatatatat acgtcttggg catgtttggc aaaactttca cttatcattt gatccgataa
     9781 tcgttttatt tccattattc tcccctccac aaaaaacata cgtctttttg atctttttgt
     9841 ttcaaataaa atgaatttac aaaaaaatat tggcaaaaac cgaatttatg ttatacttat
     9901 aaaaatttat cttgggaggc actccattgg aaacaacatt aaggaaatat atcgaagtag
     9961 caagtaaaaa aaagcctgct gacctagtga tcaagcatgc taagatcgtc aacgtcttta
    10021 cgaaagaaat catggaaaaa gatgtagcaa tctgcgaagg aatgatcgta ggaatcggtg
    10081 aatacgaagg aaaaagcgta cataatgcta atgggcaata tctagtgcca ggattcatcg
    10141 atggccatgt gcatatcgaa agctcacttt tatcaccaag cgaattttca aaagtatcct
    10201 tgcttcatgg agttactact gtagtgacag atccccatga aataggaaac attgctggga
    10261 gtacaggtct tgagtttatg atagaagatg caagacaaac actcatgaat atctttttta
    10321 tgctaccttc ttgtgtacct gtaacccctt ttgagaccaa tggtgcaaat ttaaatgcaa
    10381 aaaaattagc ccctttttta caaaaacccg aagttttagg cttagctgaa gtcatgaact
    10441 atcaagcagt agcaaataat gaaacagaca tcctagaaaa gatccaattg atgcaccagc
    10501 ataagaaaaa aattgacggg catgcagctg gtatcggcat ggaagagtta aatgtttatc
    10561 ctgcagcagg tattcggacg gatcatgaag ctacaactgc aaaagaagct aaagaacgtt
    10621 tggatcttgg tatgtattta atggttcgag aagggaccgt tgcaaaagat ctggcatcgc
    10681 tactaccggc gatcacacct gaaaattcaa gaagatgctt ctttgtcacc gatgataaac
    10741 tgatcaatga tttagtaaaa gaaggatcaa tcgatcatat cgtccgtcaa gctattgcac
    10801 ttggaatcga tccattacaa gccattcaga tggcaacatt gaatgcagca gaatgttttg
    10861 atttgaaaac actaggtgcg attgcccctg ggtatcaagc tgatttcttc ttaacagacg
    10921 atcttaccaa gctgccaatt catgaagttt tctataaggg aacattagtt gttcaatctg
    10981 ggcaactcca agaccattta tttgctgatc attcaaattc atatacgtgt tgtctgccaa
    11041 aactaaacgc tcaaccatta aatccacatt cgttagagct gcttctttct tcttcacaag
    11101 cccatgtgat cgaaattatc ccgaatagtc tagtgactaa agatttggta gaaaaagtgg
    11161 accgacaagg cgaacgattc attccttcaa tcgaaaaaga ccaattaaag attgcggtga
    11221 tcgagcgtca ccatcaaacg ggaaacattg gattaggcat agtgaaagga tttcagctga
    11281 aacaaggagc gatagcgaca acgattgccc atgactcgca taatctagtt gtcgttggga
    11341 caaacgacga ggatatgctg tatgctgcta atcttttgat tcaaaaaggt ggcgggatga
    11401 ccgttgtcaa caatcagcag gaaatagctt gtgtttctct tcctataggc ggaatcatga
    11461 cacaggaacc tttttcgatc gtctatgaac agttgaatga attaataact aaagcacatc
    11521 aactaggagc agctaaaacc tttgatcctt ttttgacact ttctttctta acattatctg
    11581 tcatacccga attgaagatc accgacaaag gattattttc attttctcgc ttccaattga
    11641 ttccaccttg tggaaaaacc gatgaaataa atacatttta aatcaaataa atcccagaaa
    11701 atacattaca tctgtaatgt tcattttctg ggatttattt atggatatta atgtttttat
    11761 gaaagcagaa agttagtttc acagactttt ctttttggtc ttatcttgaa attgagcttc
    11821 tactcgataa atacgttgac cttttgaaga aaatttttct tcatactccg tcataatatt
    11881 gccttcaaat tgactttgat gtaaatctaa ccatacctgt ttgaggatca tgccatattg
    11941 agaaaaactt gccagagaat actcaaacaa tccttgatta tctgttttga aatggatttc
    12001 tccatttgga cgaagaatct gttcatctac agcaagaaaa gtcttatatg tcaatcgccg
    12061 tttttcatga cgtttttttg gccatggatc ggaaaaattc agatagattt gatctacttc
    12121 gctgtcagcg aaatactctg tcaacgctga tccatccaca tgtagaagtt tcaggttagg
    12181 aagcggttgt tcgatcaact tgtctagtgc aatcgaaaca acactgacct gcatttctac
    12241 tccaatataa ttgatttcag gatgggcttt tgccattcca gtgatgaact ggcctttccc
    12301 cataccaatc tcaatgtgga ttggatgatc gttttcaaac aattcattcc attttccctt
    12361 ccacaatgtt ggatcactaa ttacaaaatt cgggtgagcc gctaacattt ccgctgctcc
    12421 cggacgattt cggacacgca ttatttatct ccattccttt cctctaatta aaaaggtctg
    12481 gccaaatctc cgcagagttt gggccaaacc tcagccactg aaaattcgtt ttaattgtaa
    12541 aatttcttcg ttcatccgtc gatgatcttc acgtttgcaa tatctggtga tttcttggag
    12601 caaattcatg gccgcatacc aatgaaattt ttcaagcatt tcgtcagtcg ggcgtatgcc
    12661 ataactgatc agccactgat tccagctaga cagtggcaca taatttccta aaatcgttgc
    12721 aatatccacc gcagggtctg caaacatgat tgaatcccaa tcgactagat ataaataatt
    12781 tttacatact aaccaattac gatggtttac atctccgtgt actactgata agttttcctt
    12841 atctattgac gggatctgct cacgaagaaa acgatggaca atttgcagaa attgattttt
    12901 ctggagttct tccggtaatt ctttttcata tttttgcagc atcttctccg gcgtcatgac
    12961 acgaccaccg atacgggtca gcatggtttt aagagaattt gaatgatgta aatggtacaa
    13021 aacatcaatc acgtcgtttc tttgaccaat ctcattagga gtcagcaagc gaccttccag
    13081 ccactcttgt gctgtcaaag tatcgccatt ccccgttcgt tttgtccaaa caagttttgg
    13141 tgtaattcct tcccttgaaa gagcagccaa catcggagtc gtgtttcgtt taataaagac
    13201 tttgtcctca tcacgcatgc ctatgtatgt ttttcctgtg tcgcctttga tgggacgtaa
    13261 ccgccactct tggtctaact gaaactccat catgcattct cccgtcctct tactctttgt
    13321 tttgcagtac ttattattct agtcgcctga acactacacg tcaagcacat atacgttctt
    13381 tttcatttaa ttgccattca aaaaaccagc ttttctcaaa aaaaatagaa cgaacagcat
    13441 tcttactgtc aacgctctat ttttcattct catttttacc ctacacagct ttgttttcag
    13501 tctgacatct tcttcaaacg agttggtaca tatagtttaa caaagagcat agtaacaatc
    13561 aaataagccg ctagcggaac ccatcgctgc tgcccatcta atacgactgc agcaatcaaa
    13621 ccaaaaataa ccgacgcgat aattaatacc catgtgagta gatactgaat cgcctgcttc
    13681 ttctgattct tatggattgg ataaagctga acgagaacca tatagcggaa ctggttcgct
    13741 aaaggtaaca gttggaaacc gatcaaatat aaaaataaga aaccgataat cagactaaaa
    13801 taaaagtcga ctactccggc aatcaataac ccacctataa ttactaatcg gaaataaaga
    13861 ccactaaaat cagaacctct tgccaatctt cttgcaaaca agtaaagata agtgtttttt
    13921 gattctcttt taatatgagt caatatagga tccaaatagc gtcttctttt gatctttgcc
    13981 gtaatttcag gaacatctgt gaataaattg ataaattgat agatctgatg caacctatgt
    14041 tcttctacat gcaccatttt ctcccaatcc aatccttttt cttgttgctt ccacaaaaac
    14101 cagtaatata gacttgcctg tactaatgca cctgctattc ctgcccatat tagccaataa
    14161 aatgatagaa acaaaaagat cagagaagaa aaaaaccagc ctaatttcca ataacgaata
    14221 acttgttgat ctagtcgaaa caattctgct ctcttgattt gcagatgact gaatttcaaa
    14281 ctccataata tgatcagata gacgaaaaac atcgaaaagg attgctccgt actaaccacc
    14341 actaatggca tggccaatcc gcatgccaat atcaaaaaga tcaacggaaa aatgcaagaa
    14401 tagcgaaaag ctcgttccaa ataggtttgc atttcttttt ctttaggaag taaaaaaaca
    14461 atatctgctg cctctgcaaa agtagccatc ttacccacga ataaacagag catccaaaat
    14521 acagagatta tgagccctcc aattgcaaac ggtggctgca gggttttcaa ccaattagaa
    14581 taatataatc ccacaccgcc aatcaagaaa agacaaacta aaacaaaatg atcgttcaat
    14641 acataacgca tatatttcat cattttcttt tgatgtcttg ctaaacgttt ttgaaaaaaa
    14701 ctactcatgt gcctgctcct ttgttaaagc aatatagatt tcgtccaaag aagaatccgg
    14761 cagctgaaat tcttttctca acatctcaag agaaccgtct gctcgtatct ctccgttatg
    14821 taagaccaca aaacgatcac aatatttttc tgcagtagct aaaatatgcg tagacatcaa
    14881 aataccggct ccttcttttt tcatttctac catcaattca agcaatgcat tgatagctag
    14941 tggatccaag cctaaaaatg gttcgtcgat gatatataaa gaaggctcga tcaaaaaggc
    15001 gcataagatc atcactttct gtttcattcc ctttgaaaaa ttagctggga accagtcaag
    15061 acgattagat aaccgaaaag tctccaacaa cttttctgct cttttcattg ctatatccat
    15121 aggtatatcg taggccatcg cagtgacttc gatatgttct ctcaatgtca attcttcata
    15181 taaagaagga gtctctggaa tatacccaat ttgtttgcga tattcttccg tatttttctt
    15241 taaggataga tgattgattt ctatcgttcc ttttgaagct tccagcagac cgataatgtg
    15301 cttgatggtc gtacttttcc ctgctccgtt cagtccaatc aaccctacta tctctccagg
    15361 ctgtacatcg aaactgatat cttttaacac agggagatgt ccgtaccccc ctgtgacatg
    15421 atcgataatt aaactcattc tttttcctcc gccatttatt tttatttatt ataccacgaa
    15481 gaaaatgacg gtattttgtt tttttcctta caatagcttt aacttatggt aaagttaagc
    15541 tgaagtattt attctttata gaaaggatgt tttatatgga aaactgtatt ttttgcaaaa
    15601 tcatcaatca agaaatccca agctataaaa tatatgaaga cgacaaggtc tatgcttttt
    15661 tagacatctc tcaagcaacg aaaggacaca cattagttgt gcctaaacgt catgtagcag
    15721 atatttttga atatgatccc gaacttgcag gagaggtttt ttcgcgtgtg cctaagattg
    15781 ctcaagcatt ggaaaaagct tttccagaaa tgaaaggtct taatatcttg aataacaacc
    15841 gtgaactagc ctatcaatct gtttttcatt cacatatcca tttagtccct cgctacagca
    15901 aagaggatga tttttctatt cactttgtca atcatcaaga tagttatgga tcggaagaac
    15961 taaaagctat tcaagaaaca atcgtaaagc aggtgtcctg cgatgattaa aaactttttc
    16021 aaaggactgt tatttggagc tgctgctggc actgtcggag gtctgttatt ggcgcccagg
    16081 agcgggaatg aaacaagaaa gaaactgatc gaagaattgg aagaagcaac aactctgaca
    16141 aatgatttaa ataacagcct gaatcatttt aaaagcgcat tgatcgaaac aaaagaaaca
    16201 gctgaatctg tgattcctgc tttccaagaa gcaatcaaaa aagatatcga agattttaaa
    16261 ttccaagccg aaccacgtat cacacaaatc aatgaacaag tagaaaaact aaaagctgat
    16321 ctgccagagt atcctgaaca aatataataa tgattatttt ctcaacagat ttaactatat
    16381 aagtgagaaa agtttgcgta tttcctagga atgtgatgaa attttgaatt ttgtttgaaa
    16441 ctaccacgac aacgttttta aaggaaatgg gatatggtat agttaatata taaaaataat
    16501 aactaattac aggagtgcaa attttaatga aaaaaaaatc aattattttg gcagctacta
    16561 gtgcgttggc tgttttaact ctagcagctt gttcaggcga tactaataaa gatatcgcaa
    16621 cgatgaaagg cggaacgatc actgtatctg atttctatga tgaagcaaaa cttgaatcat
    16681 cgaaccaatc cttagtacaa cggatgatca tttataaagt attcaataac aaatatggcg
    16741 ataaagtaac cgataagcaa gtggatgcag aatacgacaa acaagcgaaa tcattaggtg
    16801 atacttttga atcacaatta gaagctgctg gatatacgaa agatacttat aaagaatata
    16861 ttcgtaacaa tttagctttt gaagctggct tgaaagctca cgtagatatt acagatgacg
    16921 atttgaaaac tgcttggaaa tctttccatc cagaagtaga agcacaaatc atcaaattat
    16981 caagtgaaga tgaagcaaaa gatgtgaaaa aatcagcgga tgatggagat gatttctcta
    17041 aattagccaa agacaaatca actgatacag agacaaaaga agatggcggt aaagttaaat
    17101 tcgactcaac gacaacaact attcctgcag aagtaaaaga agcagctttc aaattgaaag
    17161 acggtgagat ctccgatgtt attactacaa caaatcctac ttcttatgcg acagaatact
    17221 acgttgtcaa aatggtgaaa aaccaaaata aaggcaatga catggataaa tacaaagatc
    17281 aactaaaaga tatcgctact gaaactaaac taagtgacaa tgcattcaca actaaagtaa
    17341 tcggtgaaga attaaaagat gcaaatgtaa aaatcaaaga tgatgccttt gaaaatgtgt
    17401 tgagtgcatt cacaacaact tcatcaagta cgaaagactc aagtgaaact acagcttcaa
    17461 caaagagttc tgacacaaaa tcaactgatt caacaaaaga atcttcaact gaagaaacaa
    17521 ctgattcatc aaaa
//
LOCUS       DANKHL010000045        17401 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000045 DANKHL010000000
VERSION     DANKHL010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.047"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(540..1565)
                     /locus_tag="PW101_001053"
     CDS             complement(540..1565)
                     /locus_tag="PW101_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2513469.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1840..3525
                     /locus_tag="PW101_001054"
     CDS             1840..3525
                     /locus_tag="PW101_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HDL2513470.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3539..4822
                     /locus_tag="PW101_001055"
     CDS             3539..4822
                     /locus_tag="PW101_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL2513471.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4892..5761
                     /locus_tag="PW101_001056"
     CDS             4892..5761
                     /locus_tag="PW101_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2513472.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5758..6594
                     /locus_tag="PW101_001057"
     CDS             5758..6594
                     /locus_tag="PW101_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2513473.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6620..7687
                     /locus_tag="PW101_001058"
     CDS             6620..7687
                     /locus_tag="PW101_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDL2513474.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7684..8511
                     /locus_tag="PW101_001059"
     CDS             7684..8511
                     /locus_tag="PW101_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL2513475.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8556..9581
                     /locus_tag="PW101_001060"
     CDS             8556..9581
                     /locus_tag="PW101_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2513476.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9578..11761
                     /locus_tag="PW101_001061"
     CDS             9578..11761
                     /locus_tag="PW101_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL2513477.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11761..12889
                     /locus_tag="PW101_001062"
                     /pseudo
     CDS             11761..12889
                     /locus_tag="PW101_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            12886..13563
                     /gene="pgmB"
                     /locus_tag="PW101_001063"
     CDS             12886..13563
                     /gene="pgmB"
                     /locus_tag="PW101_001063"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL2513478.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13647..14174
                     /gene="msrA"
                     /locus_tag="PW101_001064"
     CDS             13647..14174
                     /gene="msrA"
                     /locus_tag="PW101_001064"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2513479.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14375..15061)
                     /locus_tag="PW101_001065"
     CDS             complement(14375..15061)
                     /locus_tag="PW101_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDL2513480.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            15141..16679
                     /locus_tag="PW101_001066"
     CDS             15141..16679
                     /locus_tag="PW101_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDL2513481.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16799..17266
                     /locus_tag="PW101_001067"
     CDS             16799..17266
                     /locus_tag="PW101_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL2513482.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
BASE COUNT         5645 a         3147 c         3762 g         4847 t
ORIGIN      
        1 tctaaaataa tttgtctaac tagaagaaaa gtgggaaagg atcttaacaa catcggttcg
       61 tatccaatta aggtatcgga acgatcatct tccaagagaa gtcaatcaac aaaagtaaag
      121 atagatcatt ttttgaatgc aaaataaatt gatgggaaga tagagcggtt atttttaata
      181 ttttgctagc tattattgaa taattcctcg aataaaacaa aaccagtaga catgtctgtt
      241 attctattta tgttttagag ataagtatcc ccaaataaca tggattgaaa taagggtaaa
      301 tgtgtttaaa aaaacgaact ataaaaactt atacacaacg tggttaaaaa tagttttttc
      361 ttccctgtgg aaaaaatagt ttaacacaaa cattatagtt atccacagct atctaaaaca
      421 gatggaaaat aagaaaaatt cgctaaaata ccgggagggg gtaacgaggg atgagtgatt
      481 gaaaaaagaa cagagtaact gacttatcct cagtgtttct gttcttttat aattctgttc
      541 tacatttctt gttgttggat cgaatctgtg agagtaatct cggttgaaag aatgattctt
      601 ttttcttcag ggtattggtg gtggattagt tggaataaaa gttctgtggc tttgattcct
      661 aactgatttg tcggaatgtc tatccgagtc aactgtggcg aaaataggga ggacaataag
      721 ggatcactga atcctgcgat cgaaagatca tcaggtatct gctttccaga ttgttctgcc
      781 actttgtaaa tactcaatga ttttaatgca tcggtagaaa aaatcgcagt tggtctttct
      841 cgtagcttca ttaaatcatt cattgctgcc atgctatctt ctaagcgaaa gccgccgtct
      901 ataatccatt cattacggat atctaggtgg tgatcaaata agcattgacg gtaaccattg
      961 agacgatcat tggatacata aacttctgtc ggagcatgga gacagccgat atgacggtgt
     1021 ccattcttga tcagtgtttc caccaaatcg taactatcct ggaaattatc cgtgtccact
     1081 gagtagatgt tcgtgtagtc gtttccgact tttccgatta ccacaatagg aatattgtat
     1141 tgatccaatt caaaaagaag tgtttcatcc atcgtagagc ttaaaataat aattcctcgg
     1201 atcatttttt cttggatctt tttcgcgcat ttttctagct cttcttctgg tgtttttgcc
     1261 gtttgtagaa tcaagtcgaa gttattttgt tcagaaacgg cagaaatgga ttgaataaca
     1321 tctgaaaaaa aaggattgcc ataggtcgta tgagaagtac gatgagataa tgctagaatc
     1381 gcgtcaaaac catttcctga cagtcctctt gcaattttgc tcggttcata attcaattct
     1441 ttgattgctt gaagtacact tgcttttgct ttatcagaga tatttggctg gttatttaat
     1501 actctggaga cagttgattt tgagacatta gcttttttcg caatgtcata aatagtaggt
     1561 ttcaatttta tatgcacctc ctttttggga gcgttttaga ataattccat agtacaatag
     1621 acattctttt ttgtctatga ttttgaaaat ttaggaatag cttgcatgat catgagagac
     1681 catatttttt taagaagcta ttaattattg atacataagc tttcttattt gtatttttgt
     1741 gtatatcaaa attatttaaa aaaatgctat tgacagtaag cgttttccat aatatgatca
     1801 aatcgttggg aacgctccca aaaaagaaag gggaatccga tgtatacaaa acttttacaa
     1861 gcgatttatc gcgactcaga tttgtctgag aaaaccgcct ctatgattga acaaaaagtc
     1921 agtgtttatc agcagaagaa aggccaggca ggcaaactgc attgggacga agaagatatt
     1981 ttcttgatta cttatgggga tcaatttttt gaagagcagc aaccaacttt gcgtacattt
     2041 aagaaatttt atcaaaagta tttggcagat acatttagag ttgtacattt cttgccattt
     2101 ttcccatata catctgatga tggtttctcc gttgtaaatt atcaggcaat caacgaccgt
     2161 ttgggcacat gggaagatat agaagaaatg agaagcgaaa cacggttaat gttcgacttt
     2221 gtctgcaatc atatgtctgc cgaaagtact tggtttcagg aatatttaaa cttgaatgat
     2281 acgtacaaag atttttttat tgatttgcca ctggatacag atacaagcag cgtgactaga
     2341 ccaaggacca gcccgttact tacaacgttc attgacaaaa acgggaaaga aaaacacgta
     2401 tggacgactt ttagcgaaga tcaagtagac ttgaatttta aaaatccaga agttttattg
     2461 aaaatgatcg atattctttt gttttactta gaaaaaggcg cttcatttat tcgcttggac
     2521 gctgtgggat ttttatggaa agaactagga acttcttgta tacacctgcc agaaacgcat
     2581 aaaatcatcc aattattccg tgcaatcgct gatgaagcag ctccaggaac ggtattgatc
     2641 actgagacaa atgtgccaca caaagataat atcagttatt ttgggaatgg cgagaatgaa
     2701 gcacagatgg tctatcagtt ttcgctacca cctttgaccc ttcaagcgat tcgttcgggt
     2761 aatacaagta agttgaaaga atggttaaaa accatcgatt tcgcatcgaa aaaaacaact
     2821 ttttttaatt ttttagcctc ccatgacggt atcggattga atccggtacg aggtattctg
     2881 ccggaaaaaa cgatccttga attagtggaa cagctgaaga aagagggtgc ccgagtaaac
     2941 tacaagaaaa acccagatgg aacgaccagt ccatatgaga taaacgctac gtatctagat
     3001 gcgttaagtt ctccaacaga tacgacggaa tggagaagca aacgcttttt agctgctcat
     3061 tcatttttgg taacgttacc aggcgtgccg gcaatttata ttcaaagtgt gctgggaagt
     3121 cgaaatgatt acaaaggcgt agaaaaaaca ggagagaatc gaaaaatcaa tcggcaaaaa
     3181 tttttgttag cagatattga gaaagagttg cagacagatc cattaagaac agcagtatat
     3241 ggtgaattga cccagatgct atctattcga aaaaaagaaa aagccttcca tccgaatagt
     3301 ttgatttact tactggactc agcagattct tttatcagtt ttattcgcga agcgaaggaa
     3361 gagaaagtct tagttatcca taatttttcc aatcaatcag caggttatct tttaccaaaa
     3421 ggagattacc tagacctgtt ttctggacaa attatccatt gcagtcaaga acgaaagttg
     3481 atattccagc cgtatgaata tatgtggctg aaaataataa aataagaggt ggaaaaaaat
     3541 gaaaaaaatt attgttacta cctgtctgct agctactgga tttcttctta ctgcttgtgg
     3601 tgaaagtcaa agctctaacg aatcacaaca gacaacgatt caattcatgc actcttctgt
     3661 cgaacaggaa cgattaagtg tgatcaatga actggttgct gattttgaaa aagaaaatcc
     3721 agacatcaaa atcaagcaag tgccggtaga agaatcagcg ttcaatacaa aaatcgttac
     3781 gttagctcgt tccggtaaat taccagaagt aatggaagtc agtcaggatt ttgctaaagt
     3841 gatggataaa gatgaactga ttgatcagac ggcagtccaa aacgttatcg aggaagctgg
     3901 agaagataat tattatgacg gagccaaaaa ccttgttcgt tcagaagatg gaaaaagcta
     3961 tattgctgca ccaattagcg gctgggttca aggtatttgg tatgacaaag aaaaattatc
     4021 agaagctggt ttttcagaac cagaaaattg ggatgatatt ttaaaaattg ctcaacattt
     4081 cacagataaa gagaataaac aatatggaat tgctatgcct actgctgaag gaacaatgag
     4141 tgagcaagcg ttctctcaat ttgctttgtc aaatggagca aatgttttgg atgatgaagg
     4201 cgaggtaacg atcgatacgg aaaagatgag agaagcattg tcgttttatc aaaacttatc
     4261 ccaatacacg atgccaggct ctaatgatgt gacagaaatc aaagatgctt tcatgaatgg
     4321 aacagcacca atggcaatct attcaactta cattttgcct tctgtttatg aagcggggaa
     4381 ctcagaaaat atcggttttg ctatcccaac acaaaaagat caagctgtct atggcacagt
     4441 atctggattg acgatttctt ctggtctaga cgagaaacaa aaagaggctg ctgaagcatt
     4501 cacagctttc ttatctcaac cgaaaaacat ggaaaagtgg gtcttgatgt ctcctggtgg
     4561 tgctcaacca gtgaataaac aagtagtcga actggatggt tacaaagaaa atgaagtcat
     4621 caagtctttt ggcgagctgc catctgagat tgctagtgct tttgatgaag tacaagtctt
     4681 tggactagtc ggggaaaaga atttcacgaa aatgggagat atcacaagct caggtgtgat
     4741 cggaaaagca gtgaaccaag taactgtcgg aaacgaagat gtcgatcaag ctttagctga
     4801 cgcacaaaaa aatacgaaat aacaagaagt tctgccaatg gtgttcgaaa aaagtagata
     4861 gctattggca gattatcagg gggaggtttc aatgaatcgt ccaaggaaac attctgactt
     4921 aaaattagcc atgggacttt tagcaccaag catgctttta ttagctgttt tagtgctgat
     4981 tccaatgctt tccaatattc agatcagttt tttcgatatg ccgctgaatc ctaactcaga
     5041 cgccgttttt atcggattaa gcaattatct tgaaatttta agtgatagtg aattttatcg
     5101 ttctttaggt ttaacagttg cttacacatt cttagttgta gcaggcagta cagctttagg
     5161 attagccgta gctattttgc ttaaccgcaa gttcagattt aggaaaacgg tgcgttcact
     5221 gattttatta agttatgtta cgccatctat ttctttgatt tttgcttgga aatatatgtt
     5281 caataatagt tatggaatcg tcaatgatct gttagtagat aggttgcagc ttttcaacga
     5341 agcaccgctg tggttcgacc agccagttgc tagcttcttt atggtcgttt tgtttgctat
     5401 ctggagatac tatccgtatg cgtttatttc atttttggct attttgcaaa cagtcgacaa
     5461 aacgctttat gaagcagctg aaatggacgg tgcaaatgct tggcagaagt tcaagatcgt
     5521 gacactgccg gcaatcaaac cagtactagc aactgtgatt gttttacgat ctatctggat
     5581 gttctatatg tttacagatg tctatctact gacgaataaa gtcaatatct taggcgttta
     5641 tttgtatcag acagcctttg cgttcaatga tcttggaaaa gcagcagcta tatcgattct
     5701 attgttcatc attattgcag cggtcgtctt gctctcacga aagaaggtgg acttgaaatg
     5761 aaaaataaaa agagcaggaa aatcttattt tacgcgacga ttttgttgct attagctgtc
     5821 aatttgtttc catttttgat catgctgatg acatcattca aggattcaca agaagcaatt
     5881 gcaacgacac aaactatttt tccgaaagaa tggacttttg aacattatca ggatattttc
     5941 aatgctgaga tttttccatt catcatgtat ttcaaaaata gtttagtgat ctccttgata
     6001 gcctccgtaa ctgcagtagt gattgggatc agcggggcat acgctttgtc aaaactacgc
     6061 ttcaaagctc gctcgaaaat caatgctagc ttttacaccg tttatatgtt ttccggtatt
     6121 ttgatcgttg ttccattatt taaaatcatt tccggtctag gtttgtatga taccaataca
     6181 gcactgatta tcacgatggt cgttcaaaca cttcctactg cgatattcat gctaaaaagt
     6241 tattttgaca cgattccgac tgatattgaa gaagcagcaa tgatcgatgg tcttagccgg
     6301 atccagatca ttattcggat cattatacca ttggctattt ctggaatcat ttctgtcttc
     6361 gtttattgct ttatggtttc gtggaatgat tatttatttg cttcgatttt cctgtcgacc
     6421 tccgaaaaat ttactttacc aatcggtttg aacacattat tcagtacacc agactacatt
     6481 tggggacgaa tgatggccgc ttcattggtg accgctttac cagtcatcat tatgtatgcg
     6541 ttatctgaac gatttattaa aggtgattta gtcgaaggcg gcgtaaaagg ataaaaagaa
     6601 aagaaagagg aaagaaaaga tgaaaaaatt agttgcatta gaaccaagaa tagccggatt
     6661 caaagattac gaagaccgtg agatcgaaaa gaacgaagta cgtatccgag taattttcgc
     6721 tactccaaaa ccaggaacag aaattgtcga ctttagagga ttaagtccat ttatcgatga
     6781 agaattttca gaagaatggc gcctattctt gccaagagaa gaaggaagcc aacgcggtgt
     6841 ggtatttggc gaatttcaat taggaaatat gattgtcggt acgatcgaag aaacgggcag
     6901 tttggtgaca gagtatcaaa aaggagagtt agtctgcact tatggaccca ttcgtgaaac
     6961 gctgattaca aacggtgttg ataattataa actacgtaaa cttgatcggt tggatcaatg
     7021 gaaaaatgca gtatgttacg atcctgctca attcgcttta ggtggtatcc gggaagcaca
     7081 tgttcgagca ggtgatgctg tagcaatctt cggtttaggc gctatcgggc aaattgcgat
     7141 tcaattagct aaaaaagcag gtgctactac catcgttggg atcgatccca tagcgcagcg
     7201 tagagaatta gcagaaaaat acggtgccga ttacacgcta gatcctatga aagcagatgt
     7261 cggttttcgt ctaaaggaat tgacagataa acgtggagta gattcagtta ttgaaacgag
     7321 tggcagtgct gccgcattac aagcttcttt aagagggatc ggctatggcg ggatcatttc
     7381 ttatgttgcc tttgctaaac cttttccagc tgggtttaat cttggtcgcg aagcacattt
     7441 caataatgca aaaatcgtct ttgctcgagc atctagtgaa ccaaacccag attacccaag
     7501 atggaaccgt aaacgaatcg aggacacatg ttgggaatta ttgatgaacg gttacttgga
     7561 ttgtgaagaa atcattgatc cagttgtgcc atttgcagaa agcgcagaag cttacatgcg
     7621 ttatgtagac cagcacccag aagaaagtat caaaatgggc gttcaagtcc gaggaggaga
     7681 acaatgaaat tcggtacgca agatcaagca tttttcccaa gagacatttt agaaaaattt
     7741 agatttatca aagactgtgg atttgaagaa tacgaaatcg acggtaagtt gttaatagaa
     7801 aatatcgaca cagtcaaaca ggcaatcaaa gaaacaggga tcccagtagt gacagcttgt
     7861 aacggctacg atgggtggat cggtgacttt atcgaagaac ggcgtcttaa tggtgtacgt
     7921 caaattgctg agattttgcg tgcactaagc gaggttggag gcaaaggaat cgttatccca
     7981 gcagcttggg gaatgttcac ttatcgatta ccgccgatgg tttcaccccg aagcaaagaa
     8041 ggagactata aagcagtcag cgaatcactt cgttatttag ataaagtagc cgaagaaaca
     8101 ggtacggtta tttatttgga gcctttgaac cgataccaag accatatgat caatttgtta
     8161 gcggatgcgc gtcattacat tgactctaat caattgaaac atgtgcagat catcgctgat
     8221 ttttatcata tgaatattga agaagatgat atttcaaaag cattgactac gcaaaaagag
     8281 tatgtgggtc atatccattt agcagataat catcgctacc aaccaggatc tggcgcaatt
     8341 gattttactc gccattttca aacgctactt caaaatgagt atgaaggtta tatggtatat
     8401 gaatgtcgtt tgcgaggaga aaatgaagtg aaaatttatc aggaatcatt gcaatacttg
     8461 aaaaactgtc tggaaacagc gcgtcattta aatgatttag agaaagaata gagaaagaat
     8521 aacaataagt aaataagcaa aggagagaga aataaatgaa gaaagtagca atcataggcg
     8581 ctggacaagt agcagaaaaa gtacatgctg cttattatca aaaaagatca gatgtcttag
     8641 aattggccgc tgtggttgat ccagtcagcc agcgagcaga agacttttgc cgacgtacag
     8701 gattttctca tgcgtataca tcagttacac aaatgctgaa tgagatttct cccgatatcg
     8761 tgagtgtctg tacagccaat cgctttcact tcgaacatgt attggctgcc ctcgaagcta
     8821 atgcttcagt gatgtgcgag aaaccaccag ctatgacagc tgatcaagca agaaagatgg
     8881 cagagacagc cgataaaaac gggcagattt tggcttatgg attccagcat cgtttttctg
     8941 acgaagcaac aatgatcaag aatcatcttg atgttctcgg ggatatttat tacgtagaag
     9001 cgaatgcgct tcgtcggtct ggtgttccag gctggggaac gtttatcgac aaagaacttc
     9061 aaggaggcgg gcctttgatc gattatggca ttcacatgct tgatacagcg atgtatatta
     9121 ctggatttcc taaagtaaaa agcgttcggg cgtacagctt ccaaaaaatt ggtacgaaaa
     9181 aaaattcagg gaaattcggg aaatgggatc caagcaaatt cacagtagaa gatgcaattt
     9241 ttggcacact ggaattagaa aacggcaaga tcatccaact gaatacttcg tttgctttga
     9301 atatcagaga agacaaactg aatgtggctt tttgtggcga tcaggcaggt gctaatctgt
     9361 atccaggcga actattcgaa gatgtagatg gaaagctggt ttccttacaa aagaccacgc
     9421 cagttaatga cccacatgaa ctaagtatgg ctcattttgt gaatcgggcg ctcggtgacc
     9481 agactcagat gattgcagat ggctggcaag gatatgtcat ccagaatctg gtggaaaatt
     9541 tatatcgttc ggcagaaaca ggagaagtta ttaccttatg aaagtagaag aaaaacagtt
     9601 tgatttagag tatatcaata agtatgcgag tcagatgacc attggaaatg gattttttgg
     9661 tatcagagga agccaggagg aagactatcg gaatcaagta aggggaatgt ttgcagctgg
     9721 tgtatataac cgacctttag gagcagaatc agccgagtta gtgaatctcc cagatgtcat
     9781 cagatgggaa ataaaaatcg acggtgaaca tttttctctt gagacgatga aagttcttgc
     9841 atacgagcgg gcactagatt tagaaatggg agagcttaaa cgctatgcgc tcattgaaac
     9901 tgctcaagga aaaaaagtgg aagtcattac ttgtcgtgtt gctgatcaaa ataatctcca
     9961 tcaggcagcc ctgaaagtgg tcatcaagcc gttgaccgaa gactgccaag tagatgtgaa
    10021 aacgggtatc gatggacagc aaaccaattt tggtacacag cagttggttg aaaaagaact
    10081 gcgagtattt ggtgacgatt tgatcagtgc atcctaccag acaacagaaa gtaaaattga
    10141 tattgggatt gcagctaaat tcaataaagc aggtgtattc caagcaaaaa atcgccggat
    10201 caccgcttcc ttttcagaaa agatcagaaa aaatacgtcc tttacgctgg agaagatcat
    10261 cgtgctctct acctctcttc agcaaacaga cccgtttaag aaaagtattg cccaagccca
    10321 gaaaagcgtt agttatacag aaattttaga gcagtcgatg tcttattgga cagatttttg
    10381 gcatcagcat cggataacga tccaaggcga ttcttctttc gatcagttag cgatggactt
    10441 cgctgcttac catttacaga ttatgactcc ccgcaatgat ccaagtgtta gtgtaggggc
    10501 gaagggacta actggcgaag gttataaagg gcatgttttc tgggatacgg aaatttttat
    10561 cctgcctttc tttttatatc agtttcctga agtagcaaag aatttgctgc tgtaccgtta
    10621 tgatcgatta gaaggcgcac ggcaaaaagc aagagattat cactatcaag gtgcactttt
    10681 cccatgggaa agcgcacaca ctggtcagga agagacaccc aaatttgctg cattgaatat
    10741 caaaaccgga acaagacaag aagttgcttc agctgccgca gaacatcata tcagtgcaga
    10801 ccttgcttat gccgttcaag agtatgtcca agcaacagag gatcaatcgt ttttagaaac
    10861 aaagggaaaa acgctcattc atgaaacagc agctttttgg ctgagcagag ctgtaaaagt
    10921 cgatgatcat ttagagatac acagagtcat tggtccagat gaatacacag aatatatcga
    10981 caataatgca tatacgaact atctggcgca ttataatgtg gccttagctg ttaaatatca
    11041 agctggcgat ccgctttttt tgaaagaatg tcaaaatttt ttagataagt tatatttgcc
    11101 agtgcctaac aaagaacagc tgattccgca agatgacacg tatctcagta agccagttat
    11161 tgatttgacc acttacaaag aacgtcctgg cagccagtct attttacttg attatgcccg
    11221 tagcgaagta aatgaactcc aagtcagcaa gcaagcagat cttgtcatgc tgttatactt
    11281 attacctgag tatttttctc aaccaatcgt ttctaagaat ttgcattact atgaaaacaa
    11341 aacaatccac gactcatcgt tgagtaaagc gattcacgcc atagaggctt gccgtatcca
    11401 agaaatggaa tgggcgtatc atttattcca agaagcgtgc ctgattgatt taggaccaga
    11461 tccgcattca tcagatgacg gaattcatgc tgcttcatta ggagcactat ggctagcagt
    11521 tatatttggt ttttcaggta ttacagtaac gaatggaact ctttctattt ctccaagact
    11581 tccttcagca tgggaaaaaa tttcttttcc ttttatttgg aaaaatcaca ggttgctatt
    11641 tgttttgaca aaaaaacaaa tagtaataaa aaaagagacg aaagaaacat tacctatttt
    11701 tgtcaatgga gaccaatacc agctggaaaa agaactgatc ttggaacgga ggggaaaata
    11761 atgcgtattc tgattgcgcc agattctttc aaagaaagtc tgacagcctt agaggcagca
    11821 caagccattc aatcaggttt tcaagaagtt tttgttcagg ctgaatatga tctgctacct
    11881 ataggcgatg gaggagaagg aacggtttca tcattagcag acagtttaag gttgaagcgg
    11941 caaacagcag tcgttactaa tgcatttggc aaaaacaaag aaatccagta cgcttcagac
    12001 ggtcaaactg cgatttttga aatggcagaa atcgtcggat tgaaagatgt cccactaaac
    12061 caacaacagc cacttaggtt atccacgcga ggagtaggag aactgatacg acaattgttg
    12121 caacaaggtg ttcgacacat catcatcggc gttggcggaa gtagtaccaa cgatggcggc
    12181 atcggcatgg cagaaggtct aggttatgct ttttacgatc aagataatca aaaagtagaa
    12241 gcactgggag aaaatctagg taaaattgct acagttgata aaacgaacgt gcctattgag
    12301 ctgattaaag ccaaaatcac tgtcattact gatgttacta atcttttatg cggaaaagaa
    12361 ggcgcttctt ttgttttcgg accacaaaaa ggcttgaaag aaaatgaatt agcagtggct
    12421 gaccaagcca tgcatgattt ttatatgaga tttgcccctc aagtaatcga ggcatcgggc
    12481 agtagtgctg gtggtggaat gggtgcagga ctgttagcat ttgcttcagg gaaaatagtc
    12541 tctggcatcg actacgtatt ggatttactg gagttcgata cgggtacagc aagcagacct
    12601 cgtaatcgta ggcgaaggac gtctagatgg acaaagtcta aaaggaaaag cgccagttgg
    12661 gattgctaga agagtaccag agggcgttcc tgtgatagct atttgcggaa gtgttggtaa
    12721 aggaagcgaa aaagcagcag atgttggcat ttcggccgtt tttccgatcc ttcctgcagc
    12781 tgtgccttta caagaagctt tacagcaagc cactgataat cttcatcgca cagctaaaaa
    12841 catcgcatct ttacttcaag tcgttcattt tcaaggaggt aatcgatgaa aatcaaagca
    12901 gcactattcg atttagatgg cgttcttgta gacacagctc gttatcatta cgaagcgtgg
    12961 ctggttttag ctaaccagct gtccattcca ttcactgaga aagaaaacga gcaactgaaa
    13021 ggaatatcca gaaccgagtc actagagcga ttgctgtcat ttggaaaaat ggaacagaaa
    13081 ttctcagaaa aagagaaatc agcctttgct gagcaaaaaa acaatctata tcttcaagcg
    13141 attcaaaaaa tggatgaaac atccgttttg cctggtgcaa tagctgttct tgagtacttg
    13201 aaaaaaacaa acatcaagat cggattggga tcagcaagca aaaatgcacg cctcatttta
    13261 gaaaaaacga atttgacaag ttattttgat gtccttattg acggcacgca agtatcaaaa
    13321 gcaaaacctg atccagaagt ttttctcaaa ggagcacagc aattgaatgt accgccaaat
    13381 gcatgcttgg tcattgaaga ttcagaagct ggttgtcaag cagctttggc tggtaacatg
    13441 catgtattgg gaataggtga aaacataaat cttccttctg ctgaatatgt gattcctgat
    13501 ttaaccgttt ttgatcaagt acgttcgttt tggcatttat cagaagccgt atcacgtata
    13561 taatagtttc cgttttttct gtcacaagca aaacgttatc ctccttttgg aaatcaaggt
    13621 atgatgagaa aaaaggggga ttttatatga caaaaaaagc agttttcgca ggcggatgct
    13681 tttggtgtat ggtcaaacca tttgaagagc agccagggat catttctgtg acatccggtt
    13741 atactggcgg gcatgtacca aatccaactt atgaacaagt aacaacaggc acaacagggc
    13801 atacagaagc agtagagatc gaatatgatc cagaagttat ttcttatgat cagttggtgg
    13861 aaatctattg gcagcaaacc gatccgactg acgcattagg acagttcgct gatcgcggtg
    13921 attcttatcg tccagtgatc tactataatg atgaagaaga acgaaatatt gcagagcgtt
    13981 caaaacaagc attgcaagac agcgggcgat tcgataagcc aatcgtgaca aaaatcgaac
    14041 caagaagcga attttatcct gcagaagatt accatcagga ttattacaag aaaaacaagc
    14101 tccattacaa tctttatcga gaaggttctg gacgtgcagg tttccttcgt cgacattggg
    14161 gcgtcaagaa ataagaagcc gaaaaagcta cttatggaac accgtatatc agaggttgta
    14221 aaaccagcat tttgagctaa tactgagggt cagctgattg aacaccattt agtggagagg
    14281 ttgtgaacct gtcgcctagc tccaagtcac aaggatttgg taagagaaca ctatatatca
    14341 gaggttgtga caagtcgttt gtcacaacct tttttcaata cctaagatga aggacaatca
    14401 gcaacgcact gctcaaaaaa ggaatgaaag gcagcttttc caactttttt ttatgacaga
    14461 taaagtaaac agaaaaaacg gatattccaa acagactagc aagaaacaag atctgaagca
    14521 actcttctag agacaagcca ccactccaag aaagcatcaa cagagtatcg ccagctccga
    14581 gataggattg accatagcga aggcacacac cgcctacgat aaacgttaac agcacacttt
    14641 gccattggaa gacgccggcc tgcaaccaaa aaatccataa aattgcccaa gaacagaata
    14701 gcgtgtgaga atggaccatg aagtaaaata aatccatcaa cgaaaatagc aaagccgaaa
    14761 gcatccatat gaacgtcgaa aaatttcttg cggaagaaaa agtaaagaac caggcaaaaa
    14821 gaccaccaca taaggcttct gccaacaaat agtagaaggg caaccgacac ctgcaatagc
    14881 ggcagcgaaa tcgttggacg atgatcgaga gaatcggaat caattcatac caagaaagcg
    14941 aacgacagca tttgacacaa tgagatctag gataaataat agagtgacct aacggaatcc
    15001 gctgggcggt taaacataaa aatgatccga tacagcaacc aatgataaaa taaagtaaca
    15061 cgccatctcc cccttttcaa aaccaagata cgcaagctta aactaaattt ttgtacatac
    15121 ataaaatttg aggtgaaaaa atgaacgttt caatcggatt agatatcgga accacacaaa
    15181 ccaaagcagt tgctttcaca gatgaagccc aagaagcagc atccgcttat tttcgctacc
    15241 ctttgatcca agaaacacaa gggatggcag aacaagatca ggaattgatc tttcaaggtg
    15301 tctgcaccgt tataaaagaa gtaattaaac agttgccttc agacacaatc attcaatttg
    15361 tctccttctc ctctgcgatg catggcctga tattgttgga tgaagagaaa acaccgttat
    15421 ctcgaatgat cacttgggca gataaccgag cagcggacga agcagaaaaa ctgaaatata
    15481 ccgtgcttgg caaagaattt tatcggcaga ctgggacgcc gattcatcca atgacaccat
    15541 tgaacaaaat caagtggctg caaaacagcc aacctgaatt attcaaaaaa actgcttatt
    15601 ttgtcggtat caaagactat atcttttata gattgtttgg cgaactgatc agcgactacg
    15661 gaacagcttc aggaactgga tattttgata tccatgaatt ccaatgggca gaagaagtgc
    15721 ttgactactt gaacattcga atagagcaat tgccgcaagt ctttccttcc acctatcaag
    15781 taacaggtct accatcaaaa acagcagcag aactaggttt gccaaatgat atatcatttg
    15841 ttttgggagg ggcagacggg ccgctttcta atctagggtt aggtgctttt ggtgatacga
    15901 tcgccgcttt gaccgtagga acgagcggag cacttcgttt tattactact cagcctcagt
    15961 tccatccaga aatggagacg ttttgttatg ttctggatca gaaacattgg gtagtgggag
    16021 gagcgactag caatggtgct ggcatctttg attgggcagg gcatacattt ttacaggaag
    16081 tcgttcaatc tgctaaagaa aaaggagaaa atccatatga tgcactgctt tcggctatcg
    16141 cttttgtgcc agcgggtgcc aatggtctga tttttcatcc ctatttactt ggtgagcgag
    16201 caccgctttg ggacgcagat gcgtttggca gttttatcgg tttacagcgg caacatacag
    16261 aaaaagaaat gatgaaggcg attctagaag ggatctgtct caacctttat cggattttaa
    16321 aaggagtttg ccaggaaaat cagctcacag agatccgtgc tacaggcggg tttgctcaat
    16381 ctccgatttt tcgtcagatg atggctgatg tactgggtta cccgcttgtt ttccctactg
    16441 tcacagaagc atccgcatta ggagctgtga ttttaggctg gagaagcatc ggaaagatcc
    16501 aaagtatcag tgaagcacag gaattgatag aattgaaaga gcaagttgga gtacaagaaa
    16561 ctgatcataa aaaatatcag aaaatctatc cattatttgt ttcgacacaa aagcatttag
    16621 cacagactta tcagcaattt gctcaactgc gagaagaatt agctgattct gaaaaatgat
    16681 tggctttacg gataaaaaag cgaactgaaa atcgttctca ggtaaatgat tttgttgtac
    16741 ttttcattga aagtctacta ttattttaag tgtaagaaaa tgttaggagg caagaaagat
    16801 gaaattccaa caaaccaaag aagtattaaa tcaattagtt gcagatctaa gccaaatgtc
    16861 tgttgttgtt caccaaacac attggtacat gagaggacca ggtttcctta cattgcaccc
    16921 aatgatggat gagttcatgg atgatttgaa cgctcagtta gatgaagtat ccgaacgctt
    16981 gatcacatta gacggttcac cttattctac attgcgtgaa tttgcagata acacaaaaat
    17041 ccctgatgaa attggccgct gggatcgtac tattccagaa cgtttggaaa cacttgttgc
    17101 aggctatcgt tatctagcag atctttacca aaaaggaatc gaagtttctg gagaagaagg
    17161 cgacgatcca acacaagata tctttatcga attcaaaaca gccacagaaa aacgtatctg
    17221 gatgatccaa gctcatttgg gcaaagcgcc agagatcgac gcgtaaaaac tgttgttgaa
    17281 cttgaccgaa aaagaggttg tgaaatcagc gttttgacct gagtattaga gaaacaaacg
    17341 tcaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc agctgattga
    17401 a
//
LOCUS       DANKHL010000046        17337 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000046 DANKHL010000000
VERSION     DANKHL010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.048"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(18..563)
                     /locus_tag="PW101_001068"
     CDS             complement(18..563)
                     /locus_tag="PW101_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2513483.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(575..1267)
                     /locus_tag="PW101_001069"
     CDS             complement(575..1267)
                     /locus_tag="PW101_001069"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDL2513484.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1286..1570)
                     /locus_tag="PW101_001070"
     CDS             complement(1286..1570)
                     /locus_tag="PW101_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513485.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1583..2146)
                     /locus_tag="PW101_001071"
     CDS             complement(1583..2146)
                     /locus_tag="PW101_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2513486.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2374..3036
                     /locus_tag="PW101_001072"
     CDS             2374..3036
                     /locus_tag="PW101_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDL2513487.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3160..4356
                     /locus_tag="PW101_001073"
     CDS             3160..4356
                     /locus_tag="PW101_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDL2513488.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4457..5833)
                     /locus_tag="PW101_001074"
     CDS             complement(4457..5833)
                     /locus_tag="PW101_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2513489.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5870..6736)
                     /locus_tag="PW101_001075"
     CDS             complement(5870..6736)
                     /locus_tag="PW101_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HDL2513490.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6825..7850)
                     /locus_tag="PW101_001076"
     CDS             complement(6825..7850)
                     /locus_tag="PW101_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2513491.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7868..8542)
                     /locus_tag="PW101_001077"
     CDS             complement(7868..8542)
                     /locus_tag="PW101_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2513492.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8726..9619)
                     /locus_tag="PW101_001078"
     CDS             complement(8726..9619)
                     /locus_tag="PW101_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2513493.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9693..11390)
                     /locus_tag="PW101_001079"
     CDS             complement(9693..11390)
                     /locus_tag="PW101_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2513494.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11383..12696)
                     /locus_tag="PW101_001080"
     CDS             complement(11383..12696)
                     /locus_tag="PW101_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL2513495.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12696..16733)
                     /locus_tag="PW101_001081"
     CDS             complement(12696..16733)
                     /locus_tag="PW101_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDL2513496.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16746..17204)
                     /locus_tag="PW101_001082"
     CDS             complement(16746..17204)
                     /locus_tag="PW101_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2513497.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4867 a         3898 c         3011 g         5561 t
ORIGIN      
        1 caaatcactt tgttttttta taaaatttct gcgcctagtg tggactcaaa atgtctcaat
       61 gcccattcat gtccaacggg atcgaaactt gccacggaat ctttgaaaat atggagtttg
      121 tatcctagat tatacgcatc gactgctgta tgcaacacac agatatctgt acatacgccg
      181 gtcaaataaa tatctgtgat ctggcgttct cgcaaacgga tgtctagatc tgttccacta
      241 aaagcggaat aatgccgttt gtctatccaa taaacatttt cctcttgctc atagtcttgg
      301 tataaacttt ttaaagagcc gtacaattcc cgtccacccg ttcctatcac attatgcgga
      361 ggaaataagc ggttctccgg atgaaaggaa tcttttggat catgcgcatc aatggcaaaa
      421 acgacaaaat ccttttgatc aataaacttc tttgtatgag cagtcagctt tttttcaatt
      481 acttgtcctg ctgtcccagt agtcagcttt ccatcctctg caacaaaatc atatgtgtag
      541 tcaatcgaga tcaatgcgtt catcttagtc ctccttatac gagatgtttg acaaattcga
      601 tcaccatttc tgctgatcgt ttgcctgcgt cttcgataaa ttcgtcaaag ctttgtgtcg
      661 ccgagtgatc tgctgtgtcg ctcattgcac gtacgatcaa aaatgggata ttgaactgac
      721 gagctgtttg tccaacagcg gctccttcca tttcgcaagc taatgcttca gggaaattcg
      781 ttaagatttc tttgatttta tctggcgagt cgacaaatgt atcacctgta acgatcaatc
      841 cttcttttgc gttcaaattc gttgcttttg ctgctttaac catttcactg cgcaaatatg
      901 tgcttgcttc ataatataga ggcattcctg gtaattgtcc tgggttgtag ccaaatcctg
      961 ttgcatctgc atcaaaataa gctactttgt ctgatataac gatgtcacct acttgtaagc
     1021 cttcaccgat tccgccagct gaacctgtat tgatcaccat gttcacgccg tattgttgga
     1081 tcaacagact tgtggtaata gatgctaaga cttttcctat accggaacgt acgacgatca
     1141 cttcgtggtt acctaatgaa cctgaaataa ataaagcgcc tgcgcgttcc catgataatg
     1201 gttcgcttag gttctctctc aaaattttta cttcttcttc cattgcgccg ataataccga
     1261 ttttcatctt tttaattcct tctttctata taaatgcgat tgctaaaaaa accacacata
     1321 acattaggat aacaataata agagatttca tcaaaaaaga gttccatttg gtcgtctttt
     1381 cccgttcgct gatccttgtt ttcgtgattg gtttattctt tttgctttct ttacgataaa
     1441 agctggcaat ctttttttct tcttgctgat aagctgtttc tgcttttcgc tgttttttta
     1501 atgactcgct tgcttgtgct tgttgtcgct tgcgaagttc gcttcgcgta ataagcggtc
     1561 ctttggccat aagccgatcc ctctactttc ctttggtcaa tagttcccag tattgaatcg
     1621 cataaatcgt ctttgcatca caaatcaact gatctttcat cgcttgttgc gcctcttcaa
     1681 gagttagatg atacaactcc agtacttcgt cttcatcttg tgctaaagga ttctcgacct
     1741 tctctacccc ttgtgcatga taaatatgaa ggacttcatt tgcaaaacct ggtgaaagat
     1801 acatcgaagt aagatggctc agcgacttcg cacgatagcc agtttcttct tctaattctc
     1861 tagctgctgt catttcggga ttttgtcctt ctcctggatc gatttttcca gctggaatct
     1921 ccaaaatcac tttttctaaa ggttttcgaa attgtttgac aagaagcaga cgatcttgtt
     1981 cgtcaaaagc aatgatcccg acaccgccag gatgaaatac cagttctctc ttcgaagttc
     2041 ctccatctgg caaacgaact tcgtctacag caacatcaat aatttttccg ctatagattt
     2101 ctttgcgact gattgttttt tcttcaaatt gctcattgtt cagcatatct tctcctgctt
     2161 tctttgtaat cataactgct tttttttcat ttttcaattt ttctcaaaaa ctccttatta
     2221 gtttaccata gataaacctt agattttctc taaatataga aaatccttct ttagtccgat
     2281 actctttcta gtagtccgta tgctaaaata agtatgtaat cgaataacga aaagaattca
     2341 atactcaacg ataaatgaca tggagggttt attatgataa aacgaaattg gccgtggctt
     2401 ctagcattgg tcttgctatt tctctttgtc cgaaacaata gtttttcttt tgttctagga
     2461 cttttattga taggtgtagt tttagttttg tggggactct ttggttcgcg caggaaaaag
     2521 ggaaaacaag aagaaattcc tgcactgact gacgaacttg aaactcacta tgaaaaaagc
     2581 ggtatgacag cgaatgaaat ttcatttttt cgacaaacaa tgaatcaaac aaaaaatgag
     2641 atcaatcaat tgcaaatgaa tatgcagcag acagccaaac ttaaagcaat cgatctccgc
     2701 catgatccag tcaaagcagc gaaagcttta ttcaaagaat tggtgaaaga accaaaccga
     2761 ctgcatgaag caagtcagtt tctttatacg catctgccga atctagttga tttgacaaat
     2821 aagtataatg agatcaatga ccatgagatc aaaaacaaac agacctatga aaaactagaa
     2881 gaaagcgcgc agatcatcga ccagctgtca gctttaatcg cacaagatta tcaaaaattc
     2941 gttgctgagg atctagatga tatcgacgta gagatatctg ttgcgaaaca gagcttaaag
     3001 cgtgataata aatatgaacc agaacaaaag gattaactct tttgttcata aaagaagact
     3061 cggaacattc agatgatgtc tggctgtttc gagtactttt tctaaagtag agacattcat
     3121 gtaagaatcg agaacagatt caacaaggag gaatttttta tggatcataa accgactgaa
     3181 acaaaagatg ttacaccagt taacgataca cttgaagatt tattgaacaa cccgttttca
     3241 actccaacag atacgctgac cacttcacaa caagcggaga ttgatcagct ccaagagcaa
     3301 cagacagctg ctcgtttaat tgacaaacta ccggcggaac gccaagaaca agctaaacag
     3361 ctagcagcaa aaatcgatgc taatgatgca cagtctgtca tcagctacgg atcggctgcc
     3421 caagcaaaat taagtgaatt ctcacaatcg atgttgaatc atgtgcaagc acaagatatt
     3481 ggtcctgtag gagattcctt aactgaactg atgtatcgtc tccaagaagc caatcctgac
     3541 gaactgcgtg ccggagaagg gaacatcttc cagcgtgtct tcgggaaggt caaacaatcg
     3601 atctatgaag tgacagcaaa atatcaaaag attggtgcac aaattgacaa gatttcagtg
     3661 aaattagata aagaaaaaga cggtctgcta aaagataatc tgatgttaga acagctttac
     3721 cagaaaaaca aagactactt tgatgcactg aatatttata tcgctgctgg agaattgaaa
     3781 atggaagaac ttcaaacaac gatcattcca gaagcgatga aaagagcaga agaaactggc
     3841 gatcagatgg atgttcagat tgccaatgat tacacgcaat tcctagatcg tctggataaa
     3901 cgtacgcatg atcttcgctt agctagacag atcacgatcc agcaagcacc acaaatccgt
     3961 ttgatccaaa acacaaatca ggccttggct gaaaagatcc aagcatctat cgctacagcc
     4021 attccgctgt ggaaaaacca agtcgtgatc gcattgacct tgcttcgtca aaaagatgca
     4081 gtaactgctc aacgccaagt ctctgaaaca acaaacgact tattgaagaa aaattcagaa
     4141 atgctgaaaa tctcagccat cgaaacagcg aaagaaaatg aacgtggaat cgtggatatc
     4201 gagacccttc aaaccactca aaatgatttg atcgaaacaa tccaagagac attgcgtatc
     4261 caaaaagaag gaaaagaaaa acgtcgtcat gcagaagtag agcttggaca catggaagaa
     4321 gatctgaaac aaaaactgtt ggatttaacc caatagatca cctttcgctt gttactaaaa
     4381 caaacgccca acaacagttt attacaactg ttgatgggcg tttttcttat ctttcttcta
     4441 acttctccat tggagtctat ttctcattca tcttgctgtg tccatagcca tagccaaaat
     4501 aaatgatcag tccaataacc attacgatac cgaacacgat ccatgtcaca gtttgcagac
     4561 gaagcatcaa tacaatacaa gtcaaaatgg agacgattgg caatagcgga acgaatggca
     4621 ctttgaattc tccagctttt ggtttgccaa acatttttct caacttcagt aagcctagac
     4681 taacaagcat caaataggcg agcgccatga tattcgttag ttccgctaat acattcaatg
     4741 ggaaaaatcc ggcaaagaat acagctgcca caccagcggc ataagtcgct tttttcggtg
     4801 tacgatgctt ttgatcgatt tctgtcataa acttaggaag taagccatcc ttgctgacag
     4861 caaacagtaa acgagcaagt gaatacatca tcgagatcgt taccgtcagt agcgttaaaa
     4921 ttgcccctac agcgatcacc gcgcccactg ttccatgtcc tacatacctc attgcaaaag
     4981 ccactgggtc attgactcct agttcagtat acggaacgat tcctgtcaag atcagcgtca
     5041 cgatgatgta aaggacagta gcaatcagta tcgaaccaat gattcctctt ggcacatctt
     5101 tttgcgggtt tttgacttcc tcggctgcca ttgcaacggc atcaaatccc aagaaagcaa
     5161 aaaacacaag agctgcacca tctagcacac cggaaaaacc aaaaggcatg aatggctgcc
     5221 agttatcagg tttaacgaaa aatattccta caatgataaa caacgcgatg ataccaaatt
     5281 tgacatatac catggcatta ttcaaacgta atgctttctt ggcttcaagc gacacgatgt
     5341 aagtgacgaa tatcactacg agcaccgcaa tcaagtcaat gtatgtacca ttttccggat
     5401 tgtacccgcc agttaatgct tgtggaagtt cggcccctaa acttttcaaa aaaccttgca
     5461 tatagccaga ccatccggaa gcaacagagg aaaccgctag taaaaattcc ccgatcaaca
     5521 accaccctgt cagccaggca gcaaattcac caaatacaac atacagatag gcatatggcc
     5581 caccaatcac tggcactcgc gaagcaaatt ctgcgaaact tagtccagac aaaatgacaa
     5641 cgattgcagc taagacaaat gaaagtgaca gtgctggacc agcattttga ttggccgcaa
     5701 cccctgtcac cacaaatatt cccgtaccga tgatcgcacc aattccaagc atgatcaaat
     5761 cagacgtttt tagttctttt ttcatctcac tcgtatgatt gagattgatc tctttcttac
     5821 gaaacaaatc cattgaagtt cctccccatt ttaggttgga aaacagcgat taaactgttt
     5881 tcatataccc taaaaggata ccacctaaaa tgactaaaag gcaaccaaca ataacataaa
     5941 tgagctcttt tttcgttttt ctttcgccaa ggatgaaaat cccacctaaa gtagagataa
     6001 tgatccccat ttgtgaaagt gagaagccaa cagctaaacc aagtgatttg actgttagaa
     6061 gcatgcagat gtttcctaag ccccataata atccacagat catgtttttc cacacaaact
     6121 gatcgacttt aactttacgg aaagcaaaga agcttgcgcc taagatcatt ccgatacttt
     6181 gaggtaagat aattgacatc gcatccaaat tcgcccagtt gacgatgatg gtatatacac
     6241 cataaccaat cgtagagaag atcaatgctc taaaaccctt accaaaatca agcattttat
     6301 tatctgtatc tatttttcct tcgtcatctt gtcgggcagt caaatacgca ccgcttacaa
     6361 gaagagctaa agcaataata ccgtatacat aatctttcgt ttgtgtccat tcatggaaga
     6421 aaacggctcc cgcaatcgta ttcaaaatta attgaaaacc agtagacaat ggtagcccta
     6481 cagaaacacc catgtacttc attccatgga actgtccatt ttgtccaaca ctccaagcta
     6541 agccggataa aaatccgatc aagaaaatcc aaggggtgat tacaggacta acaacgaaaa
     6601 agacaactaa agaaaacaga aatgcaccga tcgtcattcc tagtgtttgt tgattggcat
     6661 cgccaccaat tttaccactt actaaaccaa tactacccca tgccaacata gggactaatg
     6721 ctattaaaat ttccataacc aatctccttt tatttcacat gataagaaac ttatcagcga
     6781 aaacgttaac aacacaagca ctttttttcg tttctaacga ttttctaacg tttttcatta
     6841 taaaaattta agattgggaa aaataaagtc attgttgtcc cttttccttc agttgattca
     6901 gccgtcaaat ggatacctaa tttttcggcc agattttttg ctaaataaag tcctaatcct
     6961 gttgaatgtg ttttgctaag tcgaccattt ctcccagtaa atcccttatc aaagatccgt
     7021 ttcaagtctt ccgctgggat accgatccct gaatctttaa ctgaaaggct gactccttgc
     7081 gcttcctttt ctatgctcac aatgatgttt ccgtgatccg gcgtatattt gatggcatta
     7141 ctaagcaatt ggttgaagat aaaagcgatc catttagcat cggtcaaaac agtatgatcc
     7201 tcaccaacga tctcgtagcg aatatttttt tggataaaat aatttgcctg actgcgtatc
     7261 acaggctgaa tgattccttt tagtccatat tcttggatga ggtagtcccg cgagaaactg
     7321 tcgagtcttg cataatacaa aacttgttct acgtattcat cgatttttga taattcattt
     7381 tctagtaatg tatacttttg atcgtcaata tcgaactcga tcgaacgaac tagcaattta
     7441 gaagcggaaa gtggcacttt gatttcatgg acccatgagt caatataatc tttttggtct
     7501 tgttgattac tgattgctga ttgcattact tcttggtgtt cacggatcag ctgattgata
     7561 tattcttcta ccagtttttc ttcttctgtc ctcccaccac tcaaataatt ttgcaaaggg
     7621 gaagtttttt gtatgtcggc taattttccc caccatcttc tcttgctcaa gtaaccaccc
     7681 agcagataaa aaatcagaaa gacgccctca atcaaagcta aataagcaat tgtgctcaaa
     7741 tcaactttga tgtttggcgc cagccacatc acaaagatcg tcaatgcaat aaaaaacagc
     7801 catcccaaga tcaatgtcca ctgatctttc aaatatttaa aaaaattcat cttaaccgct
     7861 catcctttca gggaacgata tatccctgtc ctacttttgt ttcgatatat ccttggatac
     7921 ctgcctgttc gatttttcgg cgtaaccgat tgatgttgac tgtcaatgta ttgtcatcta
     7981 caaatcgttc gtcatcccac agtgctcgca gcaatttttc ccgacttaag atctttccat
     8041 tgtgcttcat caaaataaat aatagacgat attcattttt gctcaaatca atgatctctc
     8101 cgttgatttc catacttccg ttgtcaacat tcaacgtaat gccattgtgg ctcatcattt
     8161 cactagataa ttctgtgtag ttgtaagaac ggcgaagtaa tgcattgatt tttgcaatca
     8221 gtacatcgat ttggaaatgt tttgtcacaa aatcatctgc ccccatgttc atagccatga
     8281 tcatatccat attcgtattc ctgctggaga taaaaatgat cggtactttc gatacttcac
     8341 gtattttttg acaccaataa tagccatcca aaacgggaag attaatatct agtaatacaa
     8401 gctgtggttc ttctctttca aagtctgaga acacttgatt gaaatcggtc gttccaaagg
     8461 tttcaaattg ccatttctca agttcctcca tgatcaattg tcgaatgatc tcttcgtctt
     8521 cgactaccat gatttttgcc ataaaaaatc cctccatctt tcttcttgct actacaatat
     8581 caaagaagcc cttgtgaaac aagttggctt acctggaaaa tgtcatttta gcaaacgtta
     8641 aaaaaaactg gaaatatgcg atttaatcgc gtttgtctct tccaagcagc ttaaatttat
     8701 tatgttattc ttaataattg gtctattatc tgttattttt ctttcgttgt ttttgtcttt
     8761 taaattttcg ataacaccaa taaccgaaaa gcgttagcac tagcaatata cctagaagga
     8821 gtataagtaa tcgataaaga tggtattttt ttgtctgttc tatctttgaa gagacttctt
     8881 ctgtaaaggg aactcgtctt cctgtcacta gtaatcggtg agtgttcacg gtgtaaggtg
     8941 tacaagtgat aagtgtgact agatcctgct tttctctaat tgctaatgta tttgtttttg
     9001 ttggaaggac aacaattttc tcaacgactt cataagcgag catccgacca tacacttcaa
     9061 gatagaatcg gtcatcttgc tccatttctt caagatccgt aaagagtttt ttctcagcaa
     9121 taccgctatg ggcggtaatc acggaatgtg tactctctcc tccagttgga aaagaggtgc
     9181 cttgcaggac tgtcgctcct ctatctaaca aaagatcatt cgtttcatca aataaaggca
     9241 agctaacatt gattttcgga atatagatgg ctccgatcat atgttgttcg taatattctt
     9301 ttcctggatt ttggacattt ctaaccgctt gctcaaaagg atctttgact tgtcccattc
     9361 ctgggattac tgatgtatga gcaagcgtct gatttttctt ttcatgcact gctaattgtt
     9421 gctcatgttt ttcttcttta tcgcgttcaa gctgcttttg ataattctcg atccttcttt
     9481 gatccaaata attgctgaga gcatctgcta aaaatggata tgtaaaaact gcagctccgg
     9541 caagaaacat cactgccata aataattgta ataaaagact gatttgggtt tgaggtctat
     9601 tacctatttt ctttttcatt ttcttcacct tcttatttaa aaaaactgca gcaaccttta
     9661 taaaaccgat tgctgcagct tatttttgtt gcttatactt cagacttcat tttagattta
     9721 cggtaccagc tgtacgctcc aatcatcaaa cttattccga ttagtaagaa tgccaagagc
     9781 ccgttcccac cagtagcagg aagtgacccg cgtttcatat tcggtactct ttctggccct
     9841 ggattttcta ctcctacgat ctgaatttct tcattatagc ttccataaga gatgataaat
     9901 tcagtaaagg ctgcctcacc tgttggaagt acataacctt ctggcgccat ggtctctttc
     9961 atttgatagg ttccataatc taacccttga acctcaaagt atccatttcc atctgcgaca
    10021 aattctgttg cttgtccgct tgttgcccac tcaattctat ctgctcctga ttcataaaca
    10081 gctaaaggcg actccccttt ataaaatatt gcgtattcag aacggtcttc attccacaat
    10141 tcgaatcttg catctttaag caattcactg cttgatgcat catatttttc aaactttctt
    10201 ccacctgttg tgactttctc tggcggctct actccttctt cataatcaaa ttcatcattt
    10261 cgtccgattt caataaccgc tgtgttgtta tggaaatcat ccgcttctag ttctgtggaa
    10321 accttcaaat tatacgtgat cgttaaatct cctccacgtg catctgctaa tctacctaaa
    10381 gtatctactt tggctactcc attttcttct gtcaaaatcg tcacgacaaa cccgttgctt
    10441 actacttcta ctatataatc ttgtcctttt gttaaaatcg tctcgccggc tcgcacttct
    10501 atttcctgat cataataagt tagaccaact gtaggcgtat ctgtgatttt gtaatttttg
    10561 ataacagcgc ttccgttttc aaggacagaa tcgatgaaat aaggaatagg tgctgtgatc
    10621 gtgtaagaag ccgtttttcc tctttctaga tcaatataag aaatcgtttc ttgttctcca
    10681 gcttcattaa taatcgttac ttgtctaagg tcgctttcct ctacattcat ctgtttatct
    10741 gcttctctga tttcgttttt cggatacaaa tgcacatctg tattgtatgt gtctttccat
    10801 gtagaaccat cccacacgcc atccaccata tcaggcatca tcactggcat cataacaacc
    10861 attggagacg cgattctgga aatatgagct ggcgaataag tttctaagaa aagatagact
    10921 ttatctctag aagaaggtgc ttcttccgtc acttggacgg tagagaatac cgcttctcca
    10981 gcttcattcg ttgtttgttt gtcaatactt gtcagtcgat attttaaaaa ccaactttct
    11041 gtgtgccgtt tttgaatcca ttcaatcgct tcggcagaat taagaccgtc ttctcgttcc
    11101 ggtgttaaag gattatctcg aatcaaatcg taatatactt ccgttacgtc aaacaaatca
    11161 aaatcgactc ctggaatatt ttcttcacca aaatccgtag aaaccaatcc gctattcggt
    11221 cgttcttccg gaacagatga gaaacctttt ttgtgtaatg taatcgtcac ttcacttggt
    11281 ttttgtgcga cttcttcggc tttgactagt tttccgttcc cgaaaatccc tattcccgca
    11341 gtaacgagta acagtacact cattcccgtg acaatcattt ttttattcat ttttttctcc
    11401 ccgttctttt tttattaata aaacagcacc taacaataaa agaattccta gtaaagaaat
    11461 caacgttctt gcttcattcg tttgcgggaa tacggaccat tttcctgatt gacctggatt
    11521 gcttggacca ctcggctctt tcggtttgtc cgttgtttct tttacggtct cgttataaac
    11581 acgtggtgtt gcggattcat aggcagagac tggaactttc ccggattcta attcagccat
    11641 ctcctgtccg ttaactacta catattgtgg ttggcctttt gcatccaccc aaaattcagg
    11701 gatgatgacc tcgattctct tacttgcttc atccatctcg taaccttcca caccttttaa
    11761 ttcttcaaaa taataggtgc cagaaggcaa gaaacgttgt cctgtcgtaa caaggccctc
    11821 tttatccgaa atgaatttgc ttacattttt gtcattcaaa ggatcattgt ctgctggttt
    11881 gatccattga tttttcaagt cattagcagg agccatatct aaataatatt tccccccttc
    11941 tatcatctga tatagagcaa aaacagcacc ttctaatggt tttccttttt cagttgttcc
    12001 tctttgacgt ccatacttga agaagtatgg atctcttaga taaccgacat ttttaggata
    12061 gatatgaatc tcttgcagtt cctcggttga ttctgtcggg tgataaattg gcaaaatagc
    12121 agcaattggg atagctgtct gatcgatatc aacatccaat ccaacttctg gatcaatcga
    12181 ttcttcgaaa atcaaataaa ctgcatcccg atcttggttt ttcttaggtg ctttgatacg
    12241 tgcaatcccg tctcctaatc cgccaaccgt atctttccca gttgttaccg aagtccctat
    12301 ttggacaagc ctttcttctg tgattagctg acggatattg tttctgttcg tttcagcgat
    12361 ttgctggacg aattctttag gagtcatgtt ttcagcagct tttttttcat gataataatc
    12421 ggtcatgtcg taaatggcaa atgtcgctcc atttagtggc accgtctgtg aaatgatatc
    12481 tgctgtttcc cctgcttcct cagagatagc caagccatca ttttcatggt attcaaattg
    12541 atcgttataa tcgatatctc gaatcatccg tttatgcaac acgaattcca cgacatcttc
    12601 ccctgattct tctgccgaca tcgcatgtgg aagaaggact gctgcaagcg gtagtaaaac
    12661 aagtaattga agcatcaaac acaacagctt tttcattacc ttgctccctc ctttttagat
    12721 tgtctgacaa acaaaccata ggctgctcct gcaagcgcca tgaagaatgc agcaagcagg
    12781 atatagaata atcgtccgga tcctcctgta gcaggcaacg gattttcagg atcgttcccg
    12841 acactaagct gtaatctgat tcgatttgtc tccaaaagtt catattctaa ctcttgctgt
    12901 agatcaggcg tctctgcttc ttcgtttgga taatacgaaa tctcaacttt tagtgtaccc
    12961 gcttcttttc ctggaactac ccgatcattt tctatgtcag gttcttctcg ataactaatt
    13021 tctaaaataa agtaacctgg aagttttcta tagctgtcag gtgacttcac ttccttgatc
    13081 cgatagaccc cttccaacaa gtctgaagca ttgaataggg aagaatcgtt tggatctggt
    13141 gccaagcctc cctcgatgat ttcatactct cctgcactat tttgtttctc aattgaaaat
    13201 tctgcgcctt caagacgtcg gctattcata ctgtcaattt tcaaaagttc caaatcgatt
    13261 ggcgctagtt catcttctaa ggttacttgt agtacaccat cgatgatttc atagccttcc
    13321 ggcaagggag cagtttcgtc ttcgatgacc gtatcttcaa tgataaactt accttcttca
    13381 ttcagtgcaa actggaacac ttgatcagat agccgatacc catttggtgc gacagattcc
    13441 gagacctcat attcaccaat agacagatgc agaggttggt cgtaatttaa atcgacatac
    13501 ttttctactc ctacggcacg agaatagaag tatccgatcc caggcaaacg tttcgattct
    13561 tctggttcta acggattacc agtgtatgct cgatacagcg ggaaggaaga atcccccact
    13621 ttcctgatat cgaaacctgc accatctaat gtatggcctt catggtctaa ttttctaact
    13681 cggatctccc gataatcaaa cgtattgtaa gcagtgaatg ctacccataa acgatcctct
    13741 tcatctgttg tatacgtgag atattgccaa ttttcttcac ctgtttgctt gagttggata
    13801 gtccctatag cattatcgta aagtcgcaag atccatgtgt cattttcatc aggttctttg
    13861 aaaccttctg ggttctggct ctcaatgatt ttgtattctg catagttggt agtttcttca
    13921 ctatccttat aaacaaatga atctggctct ccgattggat gaataacttt ctcaccatcc
    13981 acgattacat atttcccgtc ttcatctact gaatagaacg tgagtctccc tttctcttcc
    14041 tctacttcgt tcacaccaga aagccctttc gctactggat atccgatttc cagtccgtta
    14101 ccatctgttt cataaagttc aaactctgcg ccattgattg gcatattact gccttcaatt
    14161 agtttattga tattcatgtg gattggcttg aattcatttc gcatttccag tttgacatac
    14221 aactcatcat actctgtgcc ttctatgtta ggctcttgta caacagaaag cttgtgatat
    14281 tctccttcat aataggaatt cggatcttta ccttccggtc cgccgactct cgaaccagat
    14341 gtatattcat ctacaagctc gtagtgacct aacatctcat cttcgttggc tgcatatttt
    14401 ttgatttgga attgatacgt ctcatctact aactgataac gtggtggtcc agcgatctct
    14461 ctgatccaaa atatgtcgct gactgctcca gtttcatctc ctaaaatcgg catcttcgtt
    14521 tcaaagatcg ctattccgtc cgtggtagtt ttacttgttt cgattggttc ggcttcccca
    14581 ctctctggat tttggtcata taatgcaaat tcgactccag ccaacggctc gccattttga
    14641 cccttcttgc tgacttggaa attacggcct ttatgttcgt aacgaatgga agaagatggg
    14701 aaatacatcc gtccagtagt gatatcattc tcataatcct ccaggttctg aatataggcc
    14761 atattgttca ctgtgcaaaa atttccgtct tgagctcccg catcaagttt tacttggtac
    14821 ttgatcctga ccatttcccc ttcacctaaa gaaatcggag aaaatttata agcttggttt
    14881 tcccgtccga catacgttgt cggagtcgcc actacttctg gtggggcttg tccatgacgt
    14941 tcttcatccc atatttctgg ctcttggtac ttcatgccgt tttctcctgg atctagatgg
    15001 taaaactgaa tatctgccga tctgccagct ccaaatggtg agacattaaa accagtttca
    15061 tcataaagtg tggcttgctg gatcgttctc ttgaaggtag aagagatatc ctccaaggct
    15121 gtaacaatgt cattcttgtt gttcgcattg taatatcgcg gtgttacgcc atcttcttct
    15181 aaatcagcaa tatttttcaa tacatttcgt ccaatagcat ctagtcgttg tctcccgcta
    15241 acgtttccat cgattcctaa accaatggag aagatacttg gcgggttctc ttgatacttg
    15301 tttttcagcc aatgatgata agcaactgtg ttgatcgcag atgacttttc ttgcgaagta
    15361 ggactgccat tcgtattgtt attagaagaa ccatcaccaa cccagtgagt cgctttatta
    15421 tctccttctt ttacctctcc ataacgatag tgtacattta cttcatcttc tggacggtta
    15481 aagtcagatg cataggtaac tggatgagta aaccctgcat tagaagtatt cccagaaaac
    15541 tcctcaaaat ttctgaacaa aacgtcatta tcttcagcaa tcttatcttc catcacagtc
    15601 cagttgctcc aagctccatt agtgctcgta cgataacttg actgaatcgg tgcataactg
    15661 aaggtcggtg tcccatctcc cacaacgatt aggatttttt ctggatttct attttctccg
    15721 ccattgtctt tatacaatgt ttcatagcca tttttaagcc ctagtgtaag cggagttcct
    15781 ccgattggtg tacgatttaa tgctgtattc tcacttcccg tcaaacgatt gatatcattt
    15841 gtcaatggaa agatgtccga actgatttgc tcatggttat tgatagctgt actattatgg
    15901 taaaaattca ccataccgat tcgaatatcc agactcgggt cagagagcag atctttactc
    15961 atttcgttta ctgcctcaat caacgcagca tttttagttt gactattggt cccagcggtt
    16021 aattcactca tactcgctga cttatctaga acaaaaacga tatcgattgg actgatttcc
    16081 tttttctcgc ccccgatgac atctagatag acattgtaaa gggttgttgc atcttcagga
    16141 tgtagatcag atgctgtgct gcttggcaga acaggttcta cccatttttt gatgtacgcc
    16201 ccgttataat ccaagtaact gttttcaaaa ttccgttcat ttccccaaag ctgaacggtt
    16261 tgagctggaa cgtttctatc ttcttcgcct gcagttgatc cattattagt agcatagtcg
    16321 taatttcgcc agttcgctcc cccgtttgta taatcactat catcgatcaa gttctctccg
    16381 tcatatggcg agcctttgtc tccttgatag cgttcattgc tttgtgtcca atttcctaac
    16441 acttttgtat agtcattcgt tgtaaacttc gggtagcgat gctgttcgtt ttcaacttct
    16501 ccgctaggat agatatattc ataggggtct tgcgtagctg ttggcccaag acgctccaaa
    16561 ataccgttga ataactggcg ttgaatgcgc tggagtgctt gaccgctggg tacgattggt
    16621 tcgtccagct tttccatctc attttcttcg ctacgttgaa tttcagcgga atcttctgta
    16681 tcagtttcat tttcttgtaa ttcttggtta tctattcttt catcaaccac cacttgaaga
    16741 ggtagttacc cttcgttcac atcggggatc ttttctgttt caaaaatcaa tgtgccggtt
    16801 tctgcttctt ttgaataaac ttttccgaca taccattctt gaccatcgat cgtctcgact
    16861 tttaggtctt ctccatcttc atcataggag gatagacctg ttccacgaac gttttttacg
    16921 gtaccaatcc cggcggctgc cgcatcgatt gctaaacgta ttctaccttc attggcactt
    16981 tccttttttt caaaagccag atgccattca agggtttctt ctttttcaat atacttccct
    17041 tcaatttttg catagtcctc atctaagaat acctcatcct cctttgtttc tgttgactca
    17101 gcagctacat ccagggtcgt catatgatag actgactgga aatatggtcc aaatagcaaa
    17161 ctgacagtta ctaactgcaa aagcttcaat tttaaccctt tcacatcctt atactccttt
    17221 caaaaatatt tgtcttcttt cacaacaatc aacaaaacaa tgcccatcta attataattt
    17281 aatttaattt taattttaaa tcaaatgata tcctttttag ttaaaaaaca ccaatta
//
LOCUS       DANKHL010000047        17254 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000047 DANKHL010000000
VERSION     DANKHL010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.049"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(24..722)
                     /locus_tag="PW101_001083"
     CDS             complement(24..722)
                     /locus_tag="PW101_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDL2513498.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="PW101_001084"
     CDS             complement(715..2067)
                     /locus_tag="PW101_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HDL2513499.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="PW101_001085"
     CDS             complement(2290..3690)
                     /locus_tag="PW101_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDL2513500.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="PW101_001086"
     CDS             3851..4588
                     /locus_tag="PW101_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDL2513501.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="PW101_001087"
     CDS             4713..5192
                     /locus_tag="PW101_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HDL2513502.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="PW101_001088"
     CDS             5189..5956
                     /locus_tag="PW101_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513503.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="PW101_001089"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="PW101_001089"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2513504.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="PW101_001090"
     CDS             complement(6859..7461)
                     /locus_tag="PW101_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2513505.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="PW101_001091"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="PW101_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="PW101_001092"
     CDS             8142..8726
                     /locus_tag="PW101_001092"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL2513506.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="PW101_001093"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="PW101_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDL2513507.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="PW101_001094"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="PW101_001094"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL2513508.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="PW101_001095"
     CDS             10627..10806
                     /locus_tag="PW101_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513509.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="PW101_001096"
     CDS             10828..11853
                     /locus_tag="PW101_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDL2513510.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12044..13288
                     /locus_tag="PW101_001097"
     CDS             12044..13288
                     /locus_tag="PW101_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDL2513511.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13441..14388
                     /locus_tag="PW101_001098"
     CDS             13441..14388
                     /locus_tag="PW101_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2513512.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14522..15151
                     /gene="upp"
                     /locus_tag="PW101_001099"
     CDS             14522..15151
                     /gene="upp"
                     /locus_tag="PW101_001099"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDL2513513.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15290..15532)
                     /locus_tag="PW101_001100"
     CDS             complement(15290..15532)
                     /locus_tag="PW101_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513514.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15653..15725
                     /locus_tag="PW101_001101"
     tRNA            15653..15725
                     /locus_tag="PW101_001101"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15686..15688,aa:Thr,seq:cgt)
     gene            complement(15844..16482)
                     /locus_tag="PW101_001102"
     CDS             complement(15844..16482)
                     /locus_tag="PW101_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2513515.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16605..17237)
                     /locus_tag="PW101_001103"
     CDS             complement(16605..17237)
                     /locus_tag="PW101_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL2513516.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT         5887 a         3057 c         3425 g         4885 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaattgtagg aaaataaaaa ggagctgttg tttgtggtag attacaaaac
    12061 gtttgaccct gatctttggg ctgcgatcgc caaagaagaa gaaagacaag aacataattt
    12121 ggagctgatt gcctctgaaa actttgtctc cgaagcagtg atggctgcac aaggaagtat
    12181 cttgacaaat aaatacgcag aaggttatcc agggcatcgc tattatggtg ggtgtgaatt
    12241 tgtcgatatc gttgaaaatt tagcgattga ccgtgcaaaa gaattattcg gagcgaaatt
    12301 tgcgaacgtc cagcctcact ccggctctca agccaacacg gctgcttatc tcgctttggt
    12361 agaaccaggg gacacgatct taggaatgga tctttccgca ggcggacatc taacacatgg
    12421 atcaccagtc aacttcagtg gcaaaaccta tcactttgtt gcttacggtg ttgatccgac
    12481 aacagaagtt attgattata atgtggtacg tattttagct agaaaacatc aaccaaaatt
    12541 gatcgtagca ggtgccagtg cttacggacg aacgatcgac tttgcgaaat tcagagaaat
    12601 cgcagatgaa gtaggcgcaa aactgatggt agatatggca catatcgcag gattagtagc
    12661 agcaggactt catcctaatc cagttcctta tgcagatatc acaacgacta ccactcataa
    12721 aacattgcgt ggccctcgag gcggcatgat tttgacaaac gatgaagcat tagcaaaaaa
    12781 aataaatagt gcggtatttc ctggtattca aggcggccct ctagagcatg tgattgcggg
    12841 taaagcagta gcattcaaag aagcattaga tcctgcattt aaagaatata gtgaacaaat
    12901 catcgccaat gcaaaagcaa tggtcaaagt tttcaatcaa gcaattggaa ccagagtcat
    12961 ttcaggggcg acagataatc atctgatgtt gatcgatgtt cgcgagttag gtataaatgg
    13021 aaaagaagca gagagcattt tggacagtgt gaatatcact gtaaataaaa actcgattcc
    13081 ttttgaaaca ctaagtccat tcaaaacaag cggtattcga atcggcacgc cagcaatcac
    13141 tactcgcggg ttcaaagaag aagatgctgt aaaagtggct gaactagttg tcaaagcatt
    13201 acaagcaaaa gatgataacg ctcaactaga cgaagtcaaa acaggtgtac gcgaattaac
    13261 tgaaaaattt ccgttgcata aaaaataaaa atttaaattc cattagtaaa cagaaaagtt
    13321 caatgttggg cttttctgtt tattttttgg gaaaacggat tctttgacaa tatcggtgat
    13381 gctctctagt atatgaggtg taattttagt cttcacacta aatctcaggg aggaaaagtc
    13441 atgactgatc gtatctcgtt agaaaaggaa gcagtagcat tcagtgatgc aaatgcgcct
    13501 catccgcgta tctatgaact agcaccggaa gaaggaagag ttttactaga agaagtacaa
    13561 gaatcacctg tgaacaaata ttccgtcgat attgaagact tgatggtgga tactggcgaa
    13621 tggggaaaac tcaatattcg tctcgtacga ccagaaggga atatggataa attaccagtt
    13681 attttttata ttcatggagc aggctgggtt tttggcagtg ctcaaaccca tgataaattg
    13741 atcagagaac ttgcggttcg tacgaattcc gtcgttgtct ttccagaata tagtcgatca
    13801 ccagaagcaa aatatccaac agcaattgaa caaaattatg ctgtgttgca acagctgaat
    13861 aaattagcca agtcaaaaaa tctggatctc gagaatctaa tcgttgcagg agattcagta
    13921 ggtggtaaca tggctacagt catgacgatc ttaacaaaag aacgaaaggg cctcccaata
    13981 tccaagcaat tgctgtatta tccagtcaca aacgctgatt ttgatacaga ttcctatcat
    14041 gaattcggag aaaactattt tttgacaaga gaaggaatga tgtggttttg ggaccaatat
    14101 acgacgaatg aagaagagcg tgctgaaatc acagcttcgc ctttgagagc aaatataaat
    14161 gaactaaatg atcttccttc agcaatgatt ttaacgggag aagcggatgt attaagagat
    14221 gaaggtgagg cttatgcgcg caaacttcgt gatgcagggg ttcctgttac acaagtacgc
    14281 ttccaaggaa tcatccatga ttttgtcatg gtaaatgcta tggatcagac aaaggcaacg
    14341 cgtgcagcaa tggcattatc tacttcttgg atcaagaaaa aagaataagc tttgcttgtc
    14401 cgcaggaaaa cttcttttca aaaacggatt ttcttaggaa aaatggcgag tttgactagt
    14461 caatcacttg attttgtttt acaattaaaa agaaacgaca gaacaaagga gagcttaaaa
    14521 aatgggcaaa tttcaagtga tcgatcatcc attgattcaa cacaaactaa ctattattcg
    14581 ggacaaaaac tgcggaacaa aggtttttcg tgaagtagtc gatgaaatcg ctatgttgat
    14641 ggcatatgaa gtatcacggg atatgccatt ggaagacatc gtgatcgaaa caccgatcac
    14701 tgaaacaaca caaaaaacgt tgtctggtaa aaaagtagcg attattccaa tcttgcgcgc
    14761 aggtattgga atggttgacg gtatcttgga attgattcca gctgctaaag tgggacatat
    14821 cggtctttat cgagatgaag aaacattgga accacatgaa tatttcgtca aacttccaga
    14881 agacattgat gcacgtcagt tgtttgtagt agatccaatg ttggcaacag gcggatcagc
    14941 aatcatggca atcgatgcat tgaaagaacg tggcgcgtcg aacattaaat ttgtttgttt
    15001 ggtagcagca cctgagggag taaaagcttt gcaagaagcg catcctgata ttgacatcta
    15061 tacagcttct ctagatgaaa aattgaatga acatggttat atcgttcctg gactagggga
    15121 tgcgggtgac cgtttgtttg gtacaaaata agtaaatacg aaaaagggtg tgacacaact
    15181 tttgtcacat ccttttttgg tcatttttaa cgtttgatgc agaacagctt tttcacagct
    15241 ttcgtttgca tataaaaaaa ccatcccaaa aatgcagaga tggtcaaatt caaatctttt
    15301 ttagttcttt gtagttaacg attactacac ccttactttg cgtcatcaag ttcgcatttt
    15361 ctgcagtttc atcaatctca acaacagcgg aatttttcaa ttgctttgaa atcgttccag
    15421 tttgcgactt gccgtgaatt gtgaacatta cgtgagtatt gatcttgaat ttttcaccaa
    15481 tttccgatgg tacgatagtt gtatcaaatt ttccaaaagt tgccattttc ataaaacctg
    15541 cctttcacat caattatacc acgttttatg aaaaatatca tgaaattaaa ctaattttcc
    15601 atcggactct ttacaatcag ccaaatagag tatataattt cttttggata aggccagctt
    15661 agctcagttg gtagagcaac gcactcgtaa cgcgtaggtc gtaggttcga tccctatagc
    15721 tggcattaaa agagaagaca gatatatcaa ggatttttaa tccattgata catctgtctt
    15781 tttgtttaaa ggaaataaag tatgctttct tagcaataga gcatgtatta tttctataaa
    15841 aatttaggag aataaaacga ctagcaatat agctgtgaaa tcaaatatca catgaactgc
    15901 ccaacttgta atgatcgagt tgcttttaat ggcagcttgg ttgaataaca atcttgccac
    15961 cccttgtatc aaaaggatgt gtgctacagt gtgtaaaata tttccattat cataagtact
    16021 gtaatggata aatccaaaga tgattgctga caagaaacta gcgacaggaa tgctgaactt
    16081 actagaaaaa atagcgagaa aatacactga gaacaattct tctccaagta tcatgatagg
    16141 caaaataata aataataacg ggctgaaagc ctcatttatt ggattgcctg ttaaacccca
    16201 tttcaaaatt tgttgcaaaa ataccgagac tgcggcactg attactacat tgataaaaaa
    16261 atatttggct atatttttga caggagcgac aggtttcgag aacatttttt tcgtatcttt
    16321 tttaccaaaa acaagtgtaa atgctataaa tccagtgata gttaaagaaa aaatgctaat
    16381 aatggatggc gcaaatagca accctaacaa accgagtaca acgacaatag tgcccaatat
    16441 ccctttttta agttctattt tttttcctaa aatgttattc atgataataa ctcctactat
    16501 tcagtttttg aagatagagt cagtatacat gaagagttaa aataggcaat gtttttttat
    16561 ggatatttgt attgctaaaa atttatctta tatattcatt ttgattatac tacttttatt
    16621 tgtaaaaatt ttctagcaaa accaagtgct tgcaactgat taagcgtaca attttttaga
    16681 tgttccgagc gtacttcaat catttcaaag gagttgctac ttaaatcgat attttttaaa
    16741 tcagtatcaa aaaagctagc attttctaaa tcattagtag aaaacattat tttagtgagg
    16801 gaacagtcta caaaatctgc gcttgataag acagagtgct caaaggtact ttcttttatg
    16861 tttgaaccag ataaatttac atagtccatt ttacaattct cgaataacgt gttcccaata
    16921 taactttcca tcaatttagt acctgttaat ttacaattga caaatttcac gcgatgcaac
    16981 atgctttttg tcaatataca gcctgaaaaa tcacagtttt cgaatataca gtcggtaatc
    17041 tccattcttt gaaaatcatt ggaagcaaaa aatgcatact tgaaaataga tttgtaaata
    17101 attggatggc agattatttg gttttcgatg acctcttttt gtgaaaggat attatacagt
    17161 tcaaaatctt cgttttcata gatatgaaaa aaggatcctt tattcaagct tgcttcatta
    17221 actttaggaa aaatcatatt taattacctc catt
//
LOCUS       DANKHL010000048        16855 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000048 DANKHL010000000
VERSION     DANKHL010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.050"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(178..2070)
                     /locus_tag="PW101_001104"
     CDS             complement(178..2070)
                     /locus_tag="PW101_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2513517.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2067..3794)
                     /locus_tag="PW101_001105"
     CDS             complement(2067..3794)
                     /locus_tag="PW101_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2513518.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3810..4460)
                     /locus_tag="PW101_001106"
     CDS             complement(3810..4460)
                     /locus_tag="PW101_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL2513519.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4664..4960
                     /locus_tag="PW101_001107"
     CDS             4664..4960
                     /locus_tag="PW101_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2513520.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5132..5752
                     /locus_tag="PW101_001108"
     CDS             5132..5752
                     /locus_tag="PW101_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDL2513521.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5844..7010)
                     /locus_tag="PW101_001109"
     CDS             complement(5844..7010)
                     /locus_tag="PW101_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2513522.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7022..7375)
                     /locus_tag="PW101_001110"
     CDS             complement(7022..7375)
                     /locus_tag="PW101_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL2513523.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7503..8525
                     /locus_tag="PW101_001111"
     CDS             7503..8525
                     /locus_tag="PW101_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDL2513524.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8544..8705
                     /locus_tag="PW101_001112"
     CDS             8544..8705
                     /locus_tag="PW101_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513525.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(8964..9398)
                     /locus_tag="PW101_001113"
     CDS             complement(8964..9398)
                     /locus_tag="PW101_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513526.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9508..11367)
                     /locus_tag="PW101_001114"
     CDS             complement(9508..11367)
                     /locus_tag="PW101_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2513527.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11485..12645)
                     /locus_tag="PW101_001115"
     CDS             complement(11485..12645)
                     /locus_tag="PW101_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDL2513528.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12952..13863)
                     /locus_tag="PW101_001116"
                     /pseudo
     CDS             complement(12952..13863)
                     /locus_tag="PW101_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            complement(14029..15222)
                     /locus_tag="PW101_001117"
     CDS             complement(14029..15222)
                     /locus_tag="PW101_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2513529.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(15306..15506)
                     /locus_tag="PW101_001118"
     CDS             complement(15306..15506)
                     /locus_tag="PW101_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDL2513530.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(15930..16163)
                     /locus_tag="PW101_001119"
     CDS             complement(15930..16163)
                     /locus_tag="PW101_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2513531.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(16170..16601)
                     /locus_tag="PW101_001120"
     CDS             complement(16170..16601)
                     /locus_tag="PW101_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2513532.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         4736 a         3686 c         3160 g         5273 t
ORIGIN      
        1 aaatcagcgg tttgacctga gtattagaga tacaaaacgg aaaaatagct tctcatattt
       61 tgccgttttt tgagctaata ccgaaggaca gctgattgaa caccgtttat tcggaaaaag
      121 ggactgtgac acgacttttg tcacagcccc taaaaaaaat aataaatttg tactattcta
      181 tgaagcttct tcttcagaaa actgactgtt ataaagatct gcatagaatc cgtctttttc
      241 catcaatgta tcgtggtttc ctgtttctac gatcgaacca tgattcatca caataatgtt
      301 gtctgcatcg cgtattgtag acaagcggtg agcaacaacg aaacttgtcc ggttttccaa
      361 taggcggttc atcgcttttt gaatcagtat ttctgttcgt gtatctacac tagatgttgc
      421 ttcatcaagg atcaggacat ctggatttgc taagaatgcc cgcgcaattg tgattagctg
      481 acgctgccct tgcgaaatat tgcttgcatc ctcgtttaat acagtgttat atccttctgg
      541 caatttacga acaaaatcgt ctacgtgagc agctttagca gcctcaatga tttgttcgtc
      601 tgttgcatcg tcgttcccat atttgatatt gtcatagata cttcctgtaa acagccaagt
      661 atcttgcagt accattgaaa actgtgcgcg caattcatca cgtgaaagat cacgggtatc
      721 tacaccatcg taacgaatac tacctccaga aacatcgtag aaccgttcaa gaaggttgat
      781 cagtgtcgtc ttccctgcac cagtcggacc tacaatagcg accatctcac caggtttgac
      841 atccaagtta aaatcagtca ttagcaagtt atctggtgtg tagccgaatt gaacattttc
      901 aaaacgaact ttgtatggtg tatctttttc ttctggaagg ccagattttt cattggacat
      961 ctcttcttca tccaacactt caaatacccg ttcggctgaa gcaactgttg attggatagt
     1021 gttcatcaaa cttgcaagtt gagtgattgg ttgagagaac tgtgttgtat attgcaagaa
     1081 cgcttgaaca tcccctaatg tcatgttccc atttgcaact tttactccgc ctagtacagc
     1141 gacgaacaca tagcctagat ttttaacgaa attcatcaac ggcatgatga ttgccgaaat
     1201 aaattgagct ttccaaccag cggcatacag attctgattt tctttttcaa atacttcctg
     1261 gtctttttca gtatgattga atgttttaac aatggtatga ccaccatatg tttcttctac
     1321 ttggttattc aataaaccta aacttttttg ctgatctgcg aaaaacactt gtgatttcgg
     1381 cgctacgatc atcacgatga tcaagcttaa agggatcatc aataacgcga ctaacgtcaa
     1441 ttgccagctg attgaaagca tcatcacgat gaccccaatc aatgtgactg cactagttac
     1501 aaattgagtc aaattttgct gcagcgtgct tgcgatgtta tccatatcat tgattgctcg
     1561 agacatgata tccccatttg tatgtgtatc ataataggaa atcggtacac gattcatttt
     1621 ggcttccaaa tcatgacgta attcataaac tgttcgttgg gaaacacgtg tcataataaa
     1681 ttgctgtaaa aagttgaata cagcagaaat caaatacatc acgattactg tcgcaatgat
     1741 ctgcgcgatt ttatcaaagt ctatcggaaa ttttgttact tgttggcctt gttgcatctg
     1801 ttgagcgcca gccattacac ctttgaatat ttctgtggtt gctttcccca agacctttgg
     1861 tgtttggatt tggaagatca cggctgctat tgctagtaca aatacagcga tgatcgcaac
     1921 catacgttta gacatatagc caaacaatcg gcgtaccgtt ccccagaaat ttttaggctt
     1981 ttcacctttt acaccgagat ttctgccagg tccaccatgt cctggaccgc ctgctggtcc
     2041 atgagaggga cgtgtgtttg ttttactcat gctaagtcct cctctcttag ttgtgaatga
     2101 acgatttctt gataagtttc gtttgttgct aatagttctt catgtgttcc tttgccaacc
     2161 agttttcctt catctagcac aaggatcata tctgcatcca ttactgtact tactcgttga
     2221 gcaactaaaa cggtgatact ttctttcata ttcgttttca atgcttggcg aagattggca
     2281 tccgttttga aatccaacgc tgagaaggaa tcatcaaaca catagatatc tgctgttttg
     2341 actaatgccc gtgcaattgc cagacgctgt ctttgtccac cagagaagtt tcctcctcct
     2401 tgttccacat ggctatctaa gccatcttcc atctctgaaa caaaatcttt agcttgtgcg
     2461 atttctaatg ctttccaaat catttcatct gttgcatccg gcttaccata ctgcatattg
     2521 tctcggatcg ttccagagaa cagaactgct ttttgaggaa caaagcccat taattctcgc
     2581 aaattatatt gagtcatgtc actaatgtcc gttccattta tttggatcga accagattcg
     2641 atgtcatata atcgagggat caaattgact aaggtcgttt tacctgaacc agtcccccct
     2701 atgattgcga cgatctcgcc cgacttggct tggaaatcaa tatcttccaa tgcaagtttt
     2761 tctgctccat gatatcgata attgacatga ttgaaggcca atgttgcgtt ttttccagca
     2821 aaagaaactg ttttaggatt ttcagggtca tggataccga ttttttcatc caacacttca
     2881 ttaatacggt cagctgaagc ttgtgcacga ggcaccatga taaaaatagc ggaaagcatc
     2941 ataaagctga tcaagatctg catcgcataa gtcataaatg caatcagatt accgacttcc
     3001 aaagtcatat ctgctatgta gtgtccacca aaccaagtga ttgctacgtt tgtaccgctc
     3061 atgatcaatg tcatcattgg catcatcaat gcgacgatcg tattaacttt gattgccgta
     3121 tttgtataat ctttattcgc taaatcaaaa cgattttctt caaagcgtgt cttatcgaat
     3181 gctcgaatca ctctgacccc tgtcaatcct tctcgaaata ccaggtttaa acggtcagtt
     3241 ttcttttgca tacttttgaa taatggaaca gcaaagtaca tgattccgcc gatcaaaata
     3301 atcattactg ggatgacaac taagaagatt tttgtcaact gatgatcttt ttgataagca
     3361 agtatgctcg cgccaatcaa cgtgattggt gcattgatca tcatacgtaa aaacatttgt
     3421 gtaaccattt ggatttgagt cacatcattg gtcgttcttg tgatcaaaga ggccgttcca
     3481 tatttatcaa acgcatcttt tgtcagactc tctgattttt tatagacatc cgtacgcaat
     3541 tgcttaccca gtttctgtga ttcacgagta gcaaaaaatg tatttgcgat agcagctaag
     3601 atactgatca aagaaaagcc gatcatcaca aaacctgtat gccaaatata atcgacatct
     3661 ccttgcgcta cccctttatc aataatattg gaggtaagtg tcggcaaata taaatctgcc
     3721 agtacttgga tcaccatgaa aactgcagct gcaattgctg aagtcaagga gatccgtttt
     3781 acgattttta gcatctagtt cccttctttc tatttatata ctccgttttt cagccaagaa
     3841 agtttcaaat tgaattcact gattgcttgc tctactgagt aatcttcaga tgttgaaaat
     3901 aagcgatacg aatgcgcaat tgttgtaaat acagtattca tcactaattg gatcagtaat
     3961 tccaattcat gatcattttt tactttcagc ttcgtccgat cgatcaattc aagcaactct
     4021 tttgcatctt gtttgtgttc tttatgtgga tcgtgaggcg ttttttggcg actcgtatga
     4081 ggagcgactt ttcttgaggc acggtaatcc atgttcataa acaagttacg ataaaaagaa
     4141 gcattctcgc cttccaaaat ctcataaatc attttagaaa aataggattc gaatcctttg
     4201 aatagatcac catcggattc tttcattagt tgtaacatat ctttagagct gtcacgtctc
     4261 attgtctcaa agtaataaaa ataaagatcc tctttatctt caaaatactg atagaaactg
     4321 cctctaggga tatccgctaa tttgacgata ttagcaatcg aagcttcttt taagggtact
     4381 ctagaaaact cgattctcgc agcctcgatt agtctttttt gtttttcttt aggcaaatgg
     4441 aaaaatgtct gtgtcggcat atggatcctc ctttctttca agatgacaca ttgtcatatg
     4501 acgctgtgtc attataacac ctcctacctg ttttgcaaga catttgcata gtttttttca
     4561 ttatcatccg atacattaaa aaagtgataa gaggaatcct tccatttgaa ttaatgagag
     4621 ttgcccatta aaatagccac atactaaaca aaggatgaga taaatggccc aatcaattag
     4681 tgtccaagac ttcaataaat tgcctctcga tgctgacagc atcgttttag atacaagaaa
     4741 taccttagat tacacaaaaa accacttaag ccactcgctt tctatccccg ctgctatctt
     4801 gccaagacaa ttaaaaaatc tagatccaaa gaaactttat tatattctaa gctatacagg
     4861 caggcgatca gaagcattag ccgatcaact tactgctaat ggttttcgtg ctgtttatat
     4921 cattggtggg atggaagctc ttcagtcgag tgttgcttga atattgcttg ttagattcaa
     4981 aagaggctgt gacaaaaaat cgtgtcacag cctcttttat accctgtctt tttttagtta
     5041 tttattcttc aaccttatca atatgacttg atagcctttt ctttttctca tagttataac
     5101 aatcatagca ataaaaggag aaggtaagca tatggcatct gttttagtag tcaaaggtca
     5161 tcccctaact gcagaagaat cgcgcacagt taaggcattg acgtcttttt taacaagtta
     5221 taaagaaaat catccagatg atgaagtgac tgttttggaa ctttatcgcg atgatattcc
     5281 ggaaatcgat gaagaattat tatcaggttg ggaagcatta cgtgctggtg cagaattcac
     5341 ttccttatca gaaagtcaac aacaaaaaat tgctcgtttc aatgaattga ctgatcagtt
     5401 ccttgctgct gataaaatcg ttatcgcaaa cgccttatgg aatctaaata tcccaacaaa
     5461 attaaaagct tggttcgata ccgtcaatgt agctggaaaa acgttccgct atacagaaaa
     5521 cgggccagaa ggattagtga caggtaaaaa agcactgcac atccaatcta atggcggtgt
     5581 atacaacgga caagactttg cctctcaata tgtaaaaggt attttaaact ttgtaggcat
     5641 cgatcaagta gatcagctat tcatcgaagg aatcgattat gaccctgacc gtgcagatga
     5701 gttgatgcaa aatgcattag ataaagctgc tgctttaggt aaatcatttt aaaaattgag
     5761 ataaatgcat acaaaagaga cagaggaaaa aatcttcaaa gcagcatttg taccttggat
     5821 attttcccat ctgtctcttt ttgttattct ttctgctttt tcaacggatt ttcttcataa
     5881 agagaagaga ttgttgtgta ccattcaaaa acatgagtaa tcactacttt agctgctgca
     5941 taaacaggaa tccccaagat cacaccgatg attccgaaca atttcccaga agtcagcaga
     6001 actaataaaa tcgttactgg atggattgct aactgattcc ctagtactaa tggagagatc
     6061 aagcgtcctt ctattgtctg ctctactgca aaaacgatca gtactttgat gatcatgaat
     6121 ggacctgcta caatggctaa aaagatagcc ggaatcatcg ctaaaaatga tcctaagtaa
     6181 gggatcaggt tcaaaaaacc cgcaataatc cccaacgtga cagcatactc taaaccaatg
     6241 accgcaaaac caatcataaa catgattgcc acagcaaaag ccacagttag ttgtcctctt
     6301 atataagaag aaacttgact attcatttca ctcagcacat tcaacgtcgg tttttgcatg
     6361 cgtgtcggta aaaatttgac aaaataaggt gctaattgtt taccgtcttt caatagataa
     6421 aataagataa acggcatcgt cagcagagcc actaaaatag tcgctaccgc gccgacaaaa
     6481 cttccaagac tctgaactgt actcttagat atatcttgga tcatatcgcc tgccgaattc
     6541 atgaacttat cacctgctgt ttccaactgt tcgcgaaatt ggttgaacag cgggtcccgc
     6601 aaaatctcgg ttaatttatt atcaatcgta tctacatatt gagggaagtt actgatgaag
     6661 ctcactgttt gttcctgaat cttagggatg atcactacgc tcccccatgc gattagtgca
     6721 acaaccaaaa caaacagccc aatgatactg taaagccttg gtacttttcg cttttcaaag
     6781 taatcaacta ccggattcat caagtaatac aagattcctg ctaaaatgat aggcagacct
     6841 acgattgcta aaaattgcca gattggtgca aacaaatatg aaacttttgt aaataaaaag
     6901 acgatcagca atattaacaa tacaacaagt aaggctgtga ctacttgatt atttaaaaac
     6961 cagcgccaaa accacgatac cggtctgttc ttcttatttt ttccttccat aattcctcct
     7021 gttagttata aaaaatttgg cttcccaaac caatagcagg cagtctatgc ggatgaagat
     7081 agattccatt tgcaatttgt ccttcttctg gacaagcatc aaaaatcaat gatacgtgag
     7141 ctactgtctc taaattccca ttggccatcg gaccgacata atcgatcgta tatacctgat
     7201 catcaaaagc aattgttccg cctgctttca aagaaaaagg ttcgtctttt gatacttctt
     7261 gaatcaccga atattttcgt aaggcagctg ttgctgtttt atcgaacaaa atcagcatgg
     7321 gttcttcctt gtttattgct tctgtcccaa tttctgtaat ctttgcctga atcattgcac
     7381 tctctccttt aattgctttc aaaaacagta taacacattc tcattcttta gccgtgtgga
     7441 ataattatgt gatagaataa aaacgaaaac tatttcatta caattaggag gaatcagctc
     7501 atatgatcga aaagataatt ttaggaacct atacgcgtcg cgaaagtaaa ggaatctata
     7561 cgatcgacct tgatacagaa aaagaagaat tagccaacct ttcattagct gttgaagaaa
     7621 atagccctac ctatgtagca aaaagtaaag ctggcaatct ttacacagtt actagtgtgg
     7681 atggattagg tggtgccggt gcgtacgata aggattttca ttttttgaat gcggtaacag
     7741 agtctggcgc tcctttgtgt tatgtagcgg ttgatgaagc acgtcaatta ctatacgggg
     7801 cgaactatca taaaggtgaa gtaaatgtct accatatatt aggagatggc gaactgaaag
     7861 cagcagattc aatattccat caagaagcga caggtcctca taaaaaccaa gatcatgcgc
     7921 atgtccacta tacggatctg actcctgacc aacgattagt cgtatgtgat cttggtacag
     7981 atcgtgtgta tacttatgat gtgtccgaaa acggcaaatt gaatcttgta actaccttca
     8041 ctgcagagcc aggaacaggc cctcgccatc ttgtctttca tccaaatgga caattcgctt
     8101 atttatttgg tgaattagac agcacagtcc gcgtgctttc ttataataaa gaaacaggtg
     8161 cttttgaaga attacagaaa atatcgacct tgccagaaga attcgacggt gaaaatggag
     8221 gggcagctat ccgcatttca aatgatggca agttccttta tgcttctaac cgaggacata
     8281 actcgattgc tgtattcgca gtttttgaag atggttccca cattcaaaac aagcaaatca
     8341 tctcaacaga aggggatttt ccccgcgatt tcgcactaga tccaagcaat aattttgtcg
     8401 tgtgtgccaa tcagaacacc gataatctga cattatatcg ccgtcatcct gaaacaggat
     8461 tactggaatt actgaaaaaa gatgtctatg taccagaatg tgtctgtgtt tattttgatc
     8521 gttagtaaga aaaggagaat gaaatgaatc agcaagacgt attagacaga ctacgggaag
     8581 aactaaatat tcctttcttt gatgggaaaa tcgaagataa agagtatagc gaagaggaat
     8641 atcagaaact caaagccgat ttacagaact actttgaaca atatgtacag aatgtagaaa
     8701 actaatcagc tttccatact atctgaaaag aacatcagga caagactttt ccgtctatcc
     8761 tgatgttctt ttctacaaat aaaaaaccgc cgagatggtt agactcgacg gaaaaggagt
     8821 atttactcga ttttgagtaa gtacaaggtt gttgttggtt ataggtatgt cattatcata
     8881 aaggattttg atcactctat cataaaggta atattacatt tatattagat aactctagtt
     8941 tttattgtta tcaaagattt cttttatttt ttcaacaaca aattagtcat tttttggatg
     9001 gcagctgtca attggtctgt cgcttggtct tgttttgctt cagatgtatt gaatagatct
     9061 tcatctaccc ctgattcaag atcgatactt tggatcattt cttcacagcc tgctacatat
     9121 gaaagataag ctttttcaaa atttttatga atgcccattg cttttgctgg tggacgcaaa
     9181 gtactgactt tcttaagcat tgcccgatat ttttttgttc cttcttcaaa taacaggatg
     9241 atctcttcaa gagtcgattt gtccagttca cttgtttttc cttcatcaat tgcgtttctg
     9301 atcacttcat atttcggatg cattccttct ccgattgctt ctgtttcctt gacgatttga
     9361 tcgatggact gcacataata gccaatattc ggtaacatgt tcgttttcct actttctcca
     9421 ttgttgttat tatttattct acttgttttt atcctcaaat gacaagtact tgaaaaaatt
     9481 taatggtttc tatacagctc gccattctta ttcgtgctca aagaaaccat ctgctaaact
     9541 atattgtttc cctgcgacaa tgtcatattg gatcaattga tattcgtcgt aaatctcttg
     9601 ttgcgagcga ttgaattgcg cttctttata ccactgtccg ttttgatagt acagttctcg
     9661 ttcaaacgcc ggaagttcct gttctaatga taaaagcagc tgataaaagc catttgttgt
     9721 ctgattcgtc tgttccatta gattcgctgc aaaataaaac ggactggtga ttgcatcgtg
     9781 agtcgtttgc ttttctaatt ctcctttact gtcgaacata agaaattgtg tttcatgcaa
     9841 tgtttgtttg tcattttttg cttggatcgt atctgaatag atccctggaa gatgatcccc
     9901 ccaaaaaaca actaaagtcc ttctagaaag attcttcaat tcttccgtga actgcttcag
     9961 tgccgtgctg ctataggaaa tatcttgcag ataattctct aagtcaagtg tcccacttcc
    10021 atctgaagtt tttgcaggat agtctagatt atcatacttt ccatcgtagg gcatatgtgt
    10081 ctgcatcgtg actaaatgaa tgaactgagg tgttttttct tccttgagaa gtgtcattac
    10141 ttctttgtaa gctgactcat cagaaatata tggattattc tctatcgtat ccgtataggt
    10201 catcgtacgc tcgctgatga actggtcaaa tcctaatgtc tgataaacat cctctctttt
    10261 atacatcgaa gtattgtatg gatggatcgc tgtcgtctgg tatgattgtg cgtttaacgc
    10321 tgaaacgata gaaggaagct gatccatttt tggtacaagc atcgtgtaag gggtggtgag
    10381 ttgcgcatta aataacgcca tagaaaaacc agtcaatgct tcaaattcga tatttgctgt
    10441 tccaccgcca tagttttgag aaagcatatt gccactatat gtttgatcag ctacttcata
    10501 ataatctgcc aatggctcac cagatacttc tacgccgttt aatcgactag gatctgagaa
    10561 actttcactc atcacgaaca cgatgttcgg cgactcttcc tctacagttt tatttttttc
    10621 atcggctagc ttctggtatt tttcagtgat ttctttgatc ttttctttcg aatatccttc
    10681 aggttcatcc attggttcta ccttcagatt gtacaaaaac ccaccgataa aaccagtgtt
    10741 atagtagttc attttctggc tgtaaggtat ccatagtgcc gttttattat atgcttttcg
    10801 caacaaatta tcgggattgt tgaaattact gatataccct agcagtccaa ttgccacaag
    10861 cagtgtcgtt aatcggatca attggaatcg ccttcctttt ttcaggcttc gaaacatgta
    10921 ccagcaaaac aatcctatca cggttcctgc ggcagctaag atcactgtaa acagcatagt
    10981 gccggtcata tctttcagca atccgatttc agtgatcatc ttcaaatcgt ctggataaat
    11041 cggttcttgc cgataactca ttttcatata atttgcaaaa cctaatacac cgatggctac
    11101 gacatacgta agggaaccta aagggacaga tcctgctagt gcaatcagaa aaataagaat
    11161 aatcagcaag acgagacagg ctaagaagaa tttttccgta tgccaagaaa aagcaaattt
    11221 caacgcaagg tctacggata gatcattttg acaccactgc aaaaataaat tactaaaaac
    11281 aacaagaaaa aagatactga tgattgctgc tgttcctttt aaaatctttt tctgtttggt
    11341 cgaaagtctg cttatatatc ttttcaaatg cttgctcctt catttctatc aataaaagaa
    11401 tgttgcttct cttattttag ttgtacgaaa ttcgaaagta aagaacggaa agaagaatta
    11461 atgaaatcca aatattctct ttctttatgg cctttctggt ttctttaata agcggacctt
    11521 tcgtactcca gaaatttttt ctagctgttg tttcacttga ttgagctgtt caaaatcagt
    11581 actgtctatg tcaatcaata tgtagacatg ctcattctta tggttacgcg atatatctgc
    11641 gatcgctagc tcgttctcat tcagcagaga aaatatctct gctaatatat tggttttctt
    11701 ttgataaaat atagtgaatc gaaacggcgc ttggaaaaac attcgtgcgg atggataatt
    11761 gaccgcttct ctaaccgtac cgaacaaaag ataatctttt aaggcttcaa ctgccaaacg
    11821 tccgctgtct gccaaagctt cctgagtcga tccccctaga tgaggcaaca taagtatacg
    11881 gtcattttct aagaactctt cttctggaaa atcagtcaag tatttagcca gatgttgttt
    11941 ttccaatgct tgcataacag catatttatc aacaatttca cttcttccta cattgataag
    12001 tacagcatct tttttcattt tttctatatt cgcctgatcg atcaatcctt ttgtatcttc
    12061 tgtcaacggc agcatgacta cgatgtaatc cgaggtagaa agaactgttt ctaaatctgc
    12121 ttgccgaaca taatccaact gggcatcgcg gatagaataa cccaagacat ccatccctaa
    12181 gctatagcaa cttagcgcta cttttgtgcc tatcgctcca agaccaagaa gaccgatggt
    12241 tttgccttgc aattctcgtc ccacataagc acttcttttg ttttctgcct gttctaaaat
    12301 attaggacct gttagtgttt gtaccatacg acttgcttcg attatcggac gggaagataa
    12361 gagcaaacag cacaaaacaa gctcttttac tgcattagca tttacacctg gtgtattcat
    12421 gacgatcgtc ccgttttcag aagctttttc aacgttgatc gtgttcaccc caacaccagc
    12481 tcttgaaatt gctagcaaat ctggtgtaag ccattcatct ttcactttcg tactacgaat
    12541 aactattcct tcggctggca aatcatctat tattttgaaa tattcccctt gtaacttttc
    12601 tatctcttgt tctggaatag catcaatcaa atgaacgtga tacataaaac cccctcctta
    12661 ctaacaagta tctcgaaatt tctgcaatta gacaactgtt taccatttga cttcctagaa
    12721 gcaaaaagag cagttagagc agagtaggct gagtgataaa aataaggcta tgacaagtta
    12781 ctgccataag ccttattttc gagagcaaaa tattgagggt gagatatctt gataaatggt
    12841 gttccgttag ctgacattga taataagact aaatttcaaa aaagttgaaa agcaaatttc
    12901 gtcaatctct tcttatttct taaaactaag tggctttttc acaacctctt tttatctaat
    12961 aaccactgtt tccaactcaa ctaattttgc ccacgtttca atttgatctg ttgttagatt
    13021 tagcgagaca acggtatgat gaccgccacc tgcctgaatc catgaatgga caccttcatg
    13081 gaaattcggt ttagttttcc ataacacacg tgcaacaggt aaattaggtg cttcagtcgt
    13141 tggttcaaag gcttctactt cattgatcag cagtttataa tgcgtaccga aatctgccat
    13201 cgatacgact accccttctc ctgctttccc atcaaataca agacgcgccg gatcttctct
    13261 atctcccatt gataaaggag aaacaacgat tcttggtttc gtcgcagcaa gcgtcggatc
    13321 tacttccatc atatgggatt ccaagattgc ttcttggcca ctagctagct cgtaagtata
    13381 atcttccatg aatcctgtat ctttccctcg agccatgatt ttcagcaacc gatcaatcgc
    13441 tgcagttttc cagtcgcctt ccccagcaaa accgtatcct tctgccatca aacgttgtac
    13501 agctagtcca ggtagttgtt tcattccata caggtcttca aaattagtcg taaaagcgtt
    13561 atagcctcct tcttctaaga aacggcgaat accgatttcc tgctgagctt gtacttttac
    13621 atgttcctcc catgtctttt cttcataatc accatagtcg aaggtataca attccttgta
    13681 ctcttcaaat aattgatcga cttcttcgtc tgatacgcga tcgattactt gtacaagatc
    13741 tccgatacca aaataatcaa cagtccaacc aaattgaatc tgtgcttcca ctttgtctcc
    13801 ttctgtgaca cccacgtttc tcatgttgtc accaaaacga gccactttta ttccaaaatt
    13861 atatgggatt ttagattatc tttttagaag ttcttgaatg tcggtatagc ccgtgtttcc
    13921 gggcttttcc ggcatttttg atattgttag atacttacaa atgttcttat tacctctcac
    13981 catttccccc tcaaaagtag taaatggagt agtaaaaccg gccttgtgtc aggctgccag
    14041 ccgttccatt tctgcccttg cggaatcgaa tgtggcgtgt gcataatagt tcagcgtcat
    14101 ggtgatattg gaatgtccca taatgtactg caaggctttg gggttcattc ccgcattggc
    14161 taaccgggtg cagaatgtat gacgcatagc atggggcgtc gtcgttttcg gcaacgcttc
    14221 ttcatggctc ttgttgtact tcttcacaag cctgccaaac atcccgccat aatctaccgc
    14281 cgtcatggga tagccgttct gtttgagaaa caggaaattg gcatacccgt caataatgaa
    14341 cggctttgca cctttgcggt tttttatcac tcgctggaac gcctgataca cttcctcatt
    14401 catggggatt tttctgacgc cgctttgggt cttgggttcg tcaatatagt aaccgacgcc
    14461 ggtatttctc aaaagctggt ggctcacgat gattacccgg ttttcaaaat ccaaatcttt
    14521 gtccgtcagt ccgcacagtt ccgaaatgcg aagccctgtc cccagcagaa taacgattgc
    14581 gtcataatgc ttttggtaaa ccttatcacc ttgaataaag gacagaaaac tttcttcctg
    14641 tgctggtgta agaggcacct ttggttccgt atcgtcgtcc agaacgtcac taagctggaa
    14701 gtcaaacggg ttttttcgga tgcaatcgtc ctgtatggcg gtgtaaaaag ccgctttcag
    14761 ggaacgcttg tcgttattga tggtcttata agaaaggccc ttttctttca tccgaagcgc
    14821 ccattccttt gcgtcggaca gcttcacact gtcaatcggg cagctcccca gccgatcttg
    14881 ttccagaata cgcatcagcc gttcccggcc cagtttggtg ttgtgcttca cattgccccg
    14941 gtgcctgatc tgcttggcgt agagctggca gacggtcatt ttcttgccga tggtgtctat
    15001 cccgtcgtca aggtctttct ggatttcttt ctccttttcc cggagtgaaa tatcctcccg
    15061 tttacctgcc ggggtcttat ccgtaggcac taacttccat gagtaaacaa actgcggttt
    15121 tccaaaggta tcaacatatt tgtaagcata tcgtccgtct tttctctggc tctctccaga
    15181 ccgcaaaacg cgatttttgc tgtctcgtct tttttctgac atctcgtcat gctcctttcc
    15241 atgacggaaa gagccgcata tatgcttata aattattata acatatctgc ggcccttttt
    15301 cactattaga ttgtgtccaa agtgtcaatt attttctcaa actgcttgcg tttgatctgg
    15361 atgcggttgc cattcataat gacccagcca gaggccggat tttcctcggc cagcttacgc
    15421 aatttgtttt ctccgatacg gaaatattta gacgcttcct caatcgtcag cgtgtatttt
    15481 tcccaaatag gcacatcatg gttcataatg cagccccctt tcacaaattc aaaggtttgt
    15541 gtgatttagg ggcatagacg gacggctgct ggcacagctc cacgggactt tcacccccgc
    15601 gtggttctca cgcggcccta cccattgcct gcgacgcttt taacgctcgg actgtggcta
    15661 taagggagta tcatagtcct gcctgtacgt cgtcgcccgc aagccgccac gctcacattg
    15721 cggcgtggtg ttcctcgctc cgcttttccc gtagggaaaa gtatcatggc gcaccagccg
    15781 tccggctcgg cacagacaga atgtgtccgt atgccctatg atgcgccgcc gttatcttgc
    15841 gggcatattt atcaaggtgc gatcaggcca ccgggaaaac accgaaacgg ggaaagggta
    15901 agccgtttca tgctgccggg gccggttcgt cagttcgtga cgatagcgtg gatgagcttg
    15961 atttccagac ggcgcttcat ggcctcgtcc agacacacat gcggatagcc gttttcgtca
    16021 tagagcgtcc gggtacacag cttgttcatg taaccgctgt aatactgcaa aacgcgctct
    16081 acggctccgg catccccagc gcgggcggcc tcgatcaccg aaaggggcaa aagctcaatg
    16141 cccttgtagt tcgggttctt catccgcgtc taacctccct tcggcatcaa cgcctttagc
    16201 tgtttccgca gctccgataa ggtatttgtc ctgtgccgct gaacggcact ccgggacatt
    16261 cccacaaggc cgccgatctc accatctgcc atatccagaa cacagtgcag gattaaaata
    16321 ctctgctcca tgctgggcag cgcggcaaag gcttcggcaa caagctcatt gttgatatgt
    16381 aggtcatagc catgcgacga aaacgtaaag ctgtcgctgg ggtagtgatc tactgtacag
    16441 agcttgtcca aatctgcctg tgacaaagag cttagtgaca cttcacggtc acggcggcgc
    16501 ttcatttcag ccaagtagct tttggcctcg ttacgcagta ccgtcttgca aaaagcatcg
    16561 aacatgcacc gcgcttcata gtcgcggggg atagcatcca tcttctcacc ccctttctgt
    16621 gggggactta ggtggttctc tccctttccg ctaacaagac tttgacaagg ccgccagcta
    16681 cccgctaaat acctcctttt tgaaaatttc tcaaaaaatt tttttgggga cagccgggcc
    16741 gcaaaagacg acaaaaatcg cccggcaggt cgcaaaccta ccgggcgaga aaatgctttg
    16801 ctatgctgct gatttacatg gtatagtaca tactcataac cggaatagcc ctgtg
//
LOCUS       DANKHL010000049        16754 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000049 DANKHL010000000
VERSION     DANKHL010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.051"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(67..1269)
                     /locus_tag="PW101_001121"
     CDS             complement(67..1269)
                     /locus_tag="PW101_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2513533.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            1386..1901
                     /locus_tag="PW101_001122"
     CDS             1386..1901
                     /locus_tag="PW101_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2513534.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2100..2546)
                     /locus_tag="PW101_001123"
     CDS             complement(2100..2546)
                     /locus_tag="PW101_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL2513535.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2593..4071)
                     /gene="msr(C)"
                     /locus_tag="PW101_001124"
     CDS             complement(2593..4071)
                     /gene="msr(C)"
                     /locus_tag="PW101_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL2513536.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4425..5054)
                     /locus_tag="PW101_001125"
     CDS             complement(4425..5054)
                     /locus_tag="PW101_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL2513537.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(5038..5502)
                     /locus_tag="PW101_001126"
     CDS             complement(5038..5502)
                     /locus_tag="PW101_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2513538.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(5989..7257)
                     /locus_tag="PW101_001127"
     CDS             complement(5989..7257)
                     /locus_tag="PW101_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL2513539.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(7488..8225)
                     /locus_tag="PW101_001128"
     CDS             complement(7488..8225)
                     /locus_tag="PW101_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2513540.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(8292..8861)
                     /locus_tag="PW101_001129"
     CDS             complement(8292..8861)
                     /locus_tag="PW101_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDL2513541.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8989..9861
                     /locus_tag="PW101_001130"
     CDS             8989..9861
                     /locus_tag="PW101_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2513542.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            10203..11075
                     /locus_tag="PW101_001131"
     CDS             10203..11075
                     /locus_tag="PW101_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2513543.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            11072..13030
                     /locus_tag="PW101_001132"
     CDS             11072..13030
                     /locus_tag="PW101_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2513544.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(13281..14132)
                     /locus_tag="PW101_001133"
     CDS             complement(13281..14132)
                     /locus_tag="PW101_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2513545.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            14255..14722
                     /locus_tag="PW101_001134"
     CDS             14255..14722
                     /locus_tag="PW101_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDL2513546.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(14985..15467)
                     /locus_tag="PW101_001135"
     CDS             complement(14985..15467)
                     /locus_tag="PW101_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDL2513547.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(15567..16430)
                     /locus_tag="PW101_001136"
     CDS             complement(15567..16430)
                     /locus_tag="PW101_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL2513548.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         4994 a         3474 c         2987 g         5299 t
ORIGIN      
        1 aagctatttt tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct
       61 gatttcttat tcttcttcat ttgtagcaaa ataattacgg tgataaatga tacaacatac
      121 actgataaga acaatggcac ctgcaaaata aaacgcatag ctttcaccaa tatgttcacc
      181 aatcacacct gcaataaatg gtgctaaagc tccaccgaaa aagcggacaa aactatagct
      241 ggacgaagca acgtttcgtt gcagacctgg tatttccata gcaatcgtcg tcaacagtgt
      301 attgataatt ccttggaaaa atcctgctaa cacaatgctg atcggtacaa ggactggcat
      361 agaagttcca gctcctaata acagcaaaca aataatgaat aagacaaatg ctgctaaaat
      421 tgtccgataa gtcgtaaact tccgctcaag agccggtgca acgaaaatcg aagcaatagc
      481 cagaagcaca ccccagccaa aaaatacaaa accgacttct aattcagaat agccaatcaa
      541 taaaaatggc gcatacgcaa gtaaagtaaa gaatccgaaa ttatataaca atgcgattag
      601 tccaactgaa cgaagtttat gattgcttaa cgctttgaca ccttcccaaa ggcctacttt
      661 tctctttggt ttctcagttt caggtaaaag taccgctagg gctataaaac cgcaaaacat
      721 caaaaatgct accccaaaaa atggataacg ccatgaacca ctgcccaaga ccccaccgac
      781 tagagggcca acagccattc cgcagccaag tgctgcttca tacatcatga ttgctttttc
      841 tgtgttacca actaaaatac tgacgatcgt tgctaaagca gtagagacaa ataaagcatt
      901 cccgacaccc cagccggcac gtaacccaat cagcattccg atattcccag ataatccgcc
      961 aagaccagcg aatacaacga tgataaacaa tcctgtcatc aatgtttttt tcaaaccgat
     1021 ccgactagat aagaaacctg taaatagcat caccacacca gtgactaaca tgtaactagt
     1081 aaataacagg gtcgtttcag ctggcgtagc gtttaactgt gcagcaatcg tttttaagat
     1141 tgggtctact agacccactc ccataaaagc gatcatacag gtagctgcta caactagcgc
     1201 tgaacttttc ccctttgttt cttcatttgt ttgttcttgt atatctattt gattctccaa
     1261 tatttccatc cctttctctc tattagcatc atacatgatt tattttacat gtaaatatgc
     1321 acatgtaaaa cgtggaaact ttactgtgat ataataaaga aaataagacg agaggtagga
     1381 tttttatgaa tactttagcc tatatattat tatgcgtgct tttatccaaa ccacgttctg
     1441 ggtatgaatt gaagcagctg atcacaattt tttgggaagc ccatcacagt cagatctata
     1501 caacgcttgc caagttagac cagcagggtt atgtgaccgt tttggacaaa gacgagcact
     1561 ctcaaaaaaa gatctaccac ttgactgatg aaggacgtgc tcttgtagag gaatggatca
     1621 aagaggaaac tcccgctcct acgcaaaaag atgaatttct agcaaaaatc tatgcttttt
     1681 cgacgctcga taaaacaaca gctactggac tgctattcac tagagaacaa cagctgaaca
     1741 aaatcatgca aaaaaatcaa aaaaaattga atgacctctc gctagacaag aaacaagaat
     1801 ttggacgtta tgttgtgatc cagcgcagaa ttatgctttg tcagcaggaa ctccaatggt
     1861 gtcagcaagt aagagaacaa atgcagcagt tttttgagta agggataaac agaacctttc
     1921 tagaaatatc tattgaagag caaattatct tcaataaaag agcaatacag ttggaaaaga
     1981 aaaagcgatc cgaacgacag gccaaaacct atcgttcgga tcactatttt tctagaactg
     2041 cattcaagag actatgacaa aaagcatgcc aaggcctttt ttcaaataac agtgttccat
     2101 cattcattac tttgaattgc ttttagcaca cttcttgaaa gcgggccaac gatgatcaac
     2161 tgcaacggta aagcggcaat cacattcatt ttccaagcat tcatataagc agagaataat
     2221 tgatcaggta ttccttgttc catgatcaca ccgaataaag acatacatgt caccattcct
     2281 aaaaccatca agcatgaaat agttaagatc atttggacct tgctttctgg atttactggt
     2341 agtttaaagg caattttttt cgccagcaca ccgatgatga agacatccaa aatgaaagcc
     2401 accacgaatc caggcactag accaatcaac aaacttgata aatgaagatc ttgatgcagc
     2461 cataaattat acgcgctcat ccccaagacc atcaaaaaac acatcattgt tgtaaaaatt
     2521 attccttctt ttttatttgt aggcaatctg taccactcct ttaaatttgt cacgaaaatc
     2581 atcgtagcac atttaaaaat ttctcgtaag tacttttttt attttctgcc cttttatttc
     2641 ataaacttca tcagccgtac gctcgacaaa gtaagaatca tgagaagtaa acaaaatcgt
     2701 tcccggatat atttgcatta gcttctctaa agcttcgatc gttgccatat caataaaatt
     2761 agtcggttca tctaacagca acacattact tggcttagta aaaagcaaag caatcgtcag
     2821 acgggtcgct tctcccccac ttaatgtaca taacggtttc gtcaaagttt cctgagcaaa
     2881 ccctaagtga ttcagcaaac tacggacaag cgattctgaa aactctgttt gtctcatgac
     2941 aaaacgaatg atggattctt cagatgtcat ttgataaccc ttttgctgat atacttgaaa
     3001 gcttactttc ggagagagaa ggattccttc tccttgtttg cggatatgtt ctaataaggt
     3061 tgtcttacct gaaccatttt caccgacaat cgcgattttt ttacctaaag aaaactgaaa
     3121 atcaccattt accagtaatg ttctcccact tctttccaat tggacatttt gtcccatgat
     3181 gggatagcgg ctgtggagtt caagagattt tggtactgga aaacggattt gtttcatctg
     3241 ctgcggcttg gttgtttctc ctattttttg gagtcttctc tctaatgttt tcgcattctt
     3301 ttggatggct ttttgtacac tgtctttttg tttagaggaa gacaaccgat ccggtctgat
     3361 ttgttgcttt ttctttttac tggacacttt ttccaacttt tccgcttgtt ttttcttttc
     3421 ttggatcgat ttttccaatc gttttttctc tttcatgatc gactctgctt ctcgtagctg
     3481 agtctttttc tccaattcct tttgagtgag ataggcacta taattccccg aaaactctcg
     3541 gatttctccg tcttttactt cccagatttt cgatgctagc tcatctagaa aatagcgatc
     3601 atgactaaca aaaaccagcg taccataata ataacgaagc tcttcaatca gtcgatcaat
     3661 actttttcta tctagatggg ttgttggctc gtctagaagc atcccttgtt cataagaaga
     3721 aagaagacgt atcaactcga tctttcgttc ttcccctccg ctccatttcc gctctgaagt
     3781 ttcttccagt cttaactgga aatgactggc taattccaaa tttaggtcct ttgcttcagc
     3841 gagataggtt aattgaggca ggtagttgaa ggtgatctct gtttggattt tcccatgatc
     3901 gggaaaaagt tcaccggcta ttagtttgag caaagtcgat ttaccgacac cattttctcc
     3961 tatgatccct atacgatctt gctgatagac acgaagagaa tcaatagata gttctagctg
     4021 gttgccaaag ctgacttgta gatttgttat atttactgct aaattttcca tatgttttac
     4081 actcctattt catgtaaaac aacaattatc gatactaaaa aagagcagaa aaatctgttt
     4141 gcaaaaaaac tgcatcacat cttttcctac tctgaacgaa ataactatga ctaaatcacc
     4201 aaatctgtca tagctttcac ctattctact gtcgttcgaa aaaatggaca tactccatcc
     4261 atgaaattgt tgtttataca tgtcttttga atttatttag ttgttattca aaagttcgaa
     4321 acgtaatttc atcgatgcag tcatttttcc gacctccttt tttattaggt taccacacac
     4381 gattcaaaaa aagcaatagc tactgaatag taactgaaaa ccttttattc gaacgtagtg
     4441 atcttttctg gtgctaaacg aacagactcg taaccttctt ctaccgcttt tcctattgat
     4501 aaaataataa ctggaacata acggtcttta tccaaaccaa atgcctctgc caactgatct
     4561 gcttcgactc caccaattgg atttgtttca tagccatgat cacgcgcaac taacatgaat
     4621 tgcatagccg ctaagctaga atcgactttg actacatcgt tcatttcttc tcttgaaaaa
     4681 ctcttgtaat aaggaatgat cgcagctaat tgttgatctc tgacttcttt tggcatcttc
     4741 cctgattcgt aagcttggtt atagatttct tcgccatatt cgtaacattg catatcgcca
     4801 aaaatcatga ccattgcaga agaagtatca ttttgtctcg tattgaaacg gatcaaagga
     4861 cgcaagattt cttttgcttc ctcgctttca gcgaccacaa agcgccatgg ttgcatattg
     4921 acagaagaag gtgcagtcgt cgcttctttg atcatttcca gcatttcttc gtgtgggatt
     4981 ttgtatgttt catcatatac gcgaacggat cttctaccag tcataattgt tgaaaaatca
     5041 tttttttgtt ccattattat taacctcttt cgtttctgtt gttatattgc aaagttgggt
     5101 attcaattta tctaaaagtg ttagaaattg tttcatttct acatcgctga aagccgggga
     5161 tagcacggaa cctgtatctt tatggtccat ttcacaatgc tcgattttct tctgggcttt
     5221 ttctgttagc gctacataca cttcccggtt gttctgagga tttctctgac gcgaaacata
     5281 gccatttgct tccaatacct tcagatgacg agtaactgct ccttgatcaa tctgcaactt
     5341 ttcttgcaaa ctgctttgta gcaatggatt ttcttctttc agtctcatca tgatctcgta
     5401 tctagtaaga cttataccga tattttcttc aaagatctgg acgatttttt gatcggctaa
     5461 tttgatttga tataaaaggt gactaatctc ttctaaagac ataatttcct cctttctaat
     5521 aattgactcg tcaacgattg acttgtcaat aatagaataa aaaaagaaaa aagtcaaaga
     5581 atcagctgtg taataaaaaa aacgctctca atagaaaagt atagagaaaa gaaatttttt
     5641 gtagaatgaa cggaaaaaat gataaagaaa gcacaacgct aaaaccaaga ttattgattc
     5701 ttatcaaact atagaagata gctcttctgc agtagaaaat tttacaccgc caaaaatttc
     5761 cgaaatcgcc ttttgcaaga atcccgtcga atattagaaa ttggatcagt atgcttaaac
     5821 agtaataaaa aagttatgac aagatgttgt cacaaccttt tttgagtaaa cggtgttcaa
     5881 aaacttgcat ctgcggtaat aagttcaacc aaacaaaaaa catgaaaatc tgtttttgtt
     5941 tggctgttct tactacttga tgcagggcag ctttttcact acctcttttt aataaccata
     6001 aaatcctatt ccttcatatc tccagcccat cggtgttagc caatctcttt ccttagcact
     6061 caaggtatag tgatgggtac cagttttttc atttggatta aatagtcgat aaattggctt
     6121 ggaaccacca gaataaaaac ttagaccttc ataacgccaa ccgatttttg ttaaagagtt
     6181 gatctcagat tgtgccattg tgtaatgatg gtctccactg taaggattgt ataaacggta
     6241 gacaggttgt ccgctgtttg gtgcttgcca agcaatccct tcatattccc agcctacagt
     6301 tggcagataa tttttttctc cggcattcaa tgtatataaa tgttctcctg tatgacggtt
     6361 atacagacga tacatcggag aagttgaagt tggtgctgta tcatactgtg tcagattata
     6421 ggtttcgatt acattgttca atgtagaacc ataattaggc gcagtggcat aacgacctgt
     6481 tagccaagcc gtagcatctt tgtaagagtt cgtattgctt ttccatgcac cggaatagta
     6541 gtaaacgcct ggctgaaaag aagtcgtctt caataccgtt acgttatctt gcaatgattc
     6601 tttgtacgaa ggatattttc taaaagcggc attcaccgta atccattgcc cattcacata
     6661 ttccgaagta ggcatcgtaa cagattggcc attatagctt cctttaatcc caaatagatt
     6721 gtaattaggg gcagaagcca aagtactgtt cccccagcca ctttcaacga tggcttgagc
     6781 aatcatgact gatgcatata agtcattggc acttgccaaa ctttgtgcag ttgaggcaat
     6841 actatcaatg aacgcttgtt gagaattagc agatgagcga gcataaaaag catagccagt
     6901 atagttttcg aattcagaca aatctgcttg atgggtatgt gtcaatgttt gcccatctgt
     6961 caattggttc gtgatttctt ctgcattctc tggattgttc tgagcttctg ctgatgtact
     7021 gtcttcggtt tgtgtttgtt ctgttgtttc agttgttggt tcttcggttg aaattgtatt
     7081 ttctgagaca gtagctgact gcaaagtact ctctgtatcg ttttcaattc ctgtttctga
     7141 agaactcgat gtctgagttg cgacagtttc tgtcgattct tcagctagca ctgttgtgtt
     7201 ggcaactgct agatttccta aaagaataac gctaaataat aaattctttg ctttcatctt
     7261 ttttctcctt tgaattatga aacttttcct aaataacagt gtaactaaaa aagtaaaagc
     7321 aagtcaatgt ccatatttaa ataacatttg tttctttttg ataaatttgc tttttctaca
     7381 taaaaagacg taaatgaaga atattcttct ccattcacgc cttaaattga tggtcgtttg
     7441 aataactatt taacaagtaa ttctctttgg atattatccg ttatggatta tacttcctgt
     7501 gtcgtcggac ggataatcat gttactgaca gacatccggt ctggcgtttc tatcgcaaat
     7561 gctacagcag aagctatatc ttcggaggtc aatccccatt ctttttgcgc ttcatgaagt
     7621 tcaagagcag cctttttgtc gctgatggtt tgatataatt cagtttgaac agctcctgga
     7681 gagatgatcg tagatttgat gttattttgc cgttgctctt gacgtaatcc ttccatgatt
     7741 gcacggacag caaattttgt tccgcaataa acggcggaat caggataaac aacatgtccc
     7801 gccacagaat cagttgaaat gatatgccca gatttttgtt tttccatgat tggaagtacg
     7861 gcggaaattc catttaacac acccatgatg ttgatatcaa gcatattttg ccattcatca
     7921 cggtgacctt caactaatgg agcggttggc atgattcctg cattattgta caatacatcg
     7981 attcgaccga acttttccat cgctaaatcg ataactgctt ggacttgtgc aaaatctctt
     8041 acatctgctg tttgaatata tagctcagct tctggtaaag attcttttat tgcttttagg
     8101 cgatcttctc ggcgtgcagc tataactagt ttagcccctt tttctgctaa tagcttcgtt
     8161 gttgcttcac cgattccgct tgaagcgccc atgatgacaa tcactttttc tgataaatca
     8221 ttcattctat tctcctcctg ttttgaaaaa tctcttgctt atactcacta tctcttattt
     8281 catcttactg ttcaaataat ccgttcgaca ggtttgacga ttttggcagg gattcctgca
     8341 acgatggtat tgtctgggac atctttcgta acagttgcat tcgctgcaac aatggcattc
     8401 tcgccaatcg ttacgcctgg cagaatagtt gctcctgctc cgatccaagc atttttcttg
     8461 atgtggattg gctttactaa caagcctctt cgttcttttg gatcggttag atggttcacc
     8521 gtgatgaggt tgacacgagg accaatcaat actgaatctt ctaaacagat gccacctaag
     8581 tcaacgaaca tcacttgacg attgataaag acatttttcc ctatactaat atgtttgccg
     8641 aaatctgtat aaaaaggcag cagaacagtc accgtatcat ctactttgtc actaatgata
     8701 ttttctagcc gtttgcggat atctgctggt tcatggtacc cagtattcag cgctgctaaa
     8761 ggctcttcgt tttcagcttg gatctgatgg atcacttcaa agagcggatc cgttactcga
     8821 atctctttat tgacgatttg tttttcgatg aggcttgtca ttttgctccc tccttttatc
     8881 cttactttat aacggacggc ttatcatgtc taatacttat aaaacatact taactattcc
     8941 ttttaactat acttagtggt atgctttttt aaaaacagga gggaaaaaat ggatattcgt
     9001 atactgacgt attttttagc agtagctaga gaaaaaaata tcagccgagc agcaaaatct
     9061 ctgcatatca cgcaacctac tctctcaaaa caactgaaag atttagaaga agaacttggt
     9121 gtgacacttt tcactcgggg ctcccgagag atacagttga cggattctgg tcgatatctt
     9181 tattcaaaag gaaaagaaat cttgtcgttg gtagaaaaaa cggcgaacaa tttaacaaat
     9241 gacgcaatca tctctggaga aatctacatt ggcggaggcg aaactaaagc aatctctttc
     9301 atttctgata gccttcattc gttgatcaag gcgcatcctg atatccagtt tcatttatat
     9361 agcgggaatg cagatgatat catagacaaa ctggatagcg gattacttga ttttggactc
     9421 gtaatcgaac cgacaaacaa acaaaaatac cagtcatttc gactgccagc ccaagatcgt
     9481 tggggacttt tgatacatcg aaatcatcct ttagcaaagc aaaccgtcat ccatccgcag
     9541 gaactgaaaa ctgttccttt gatcatttcc cggcaaacgt cagtggacag tcaactagct
     9601 gaatggctag gtttcagcat agatcagtta tcgatcgtcg gaacatataa cttattgtac
     9661 aatgcttctt tgatggtcga taacgggact tttggtgcat tagctctaga tgggatcatc
     9721 aatacttatg ggacggaaaa aatattcatc ccactcgatc cgccattaca agcttcactg
     9781 aatctcattt ggaaaaaaga ccagcctctt tctcctgctg ctcgtaaatt tttagatgtc
     9841 ctaacggaga aatacagtta agtagagttt ttttccttcg ctttcagctt tttaacaacg
     9901 aataagataa gcaaaagatc caacgattaa ataacataag tttcttaatt tatttcttcc
     9961 ttttatttct gataattcca gtaaattact ataattataa aaaaagaatt gacaaaacat
    10021 aactgtctat ataaaattga ttacgttaac aataattcgt ttcatgtgac tagatcatac
    10081 taggcatgaa aaatctctga ggaacatggt ttccctagga ggtttttcgt gtctattttt
    10141 gtttggccgc aatcaactgg tctagcaaac gtgaaaacca aatcttcaaa aagggtgcgc
    10201 gcatgaaaat caaaaagatc tttaatcaaa atgcagtatt ggtagatgac ggtggacagg
    10261 aaaaagtagc tattggaaaa gggattggat ttgacagaaa aagaaatgat ttgatttttg
    10321 atcgtgatgt cgaacggata tttgtcatgg aaccagaagg tcaaatcaag ttgcaaaatt
    10381 tacttaatca gattgatgaa cgctttttat ttgcagcaga aacgatcatt gatcatgcgg
    10441 aaactgtttt gatggaaaag ttgaatgaac atgtattgat cgctttgacc gaccacattg
    10501 ctttttctgc ggagaacatt aataacggta tcgtcatccg caacaaactt ttaagagaaa
    10561 tcgaagtgct ttacagtgat gaattcagta ttgctcaatg ggcagtagag tatctgacaa
    10621 aagagcttgg gattccatat acttatgacg aagcaggata tatcgcaatc catattcaca
    10681 gcggacgaag cggtcggaac aataaccatc gcagtatccg tgaagtgacg attgtatctg
    10741 acatcatcca tttagtggaa tcagaattgg aaacagatat ccattcagaa ccattttcac
    10801 tgaattattc aagactggtg aatcatttac gactgcttct acaacgaacc catgcgcaac
    10861 aatatgctgt tttagacacg gaaatcgtag aaatggtcaa acgaaaatac ccagaaagct
    10921 ataagatttc taaaaaaatc cgtgtgttgc tgaccaaaga ataccagctc gcgatcacga
    10981 aagaagaact gggttacttg gccatccata tcgagcgttt aagaagcgca atcgtacaaa
    11041 caaatgttaa caatcatgag gaggaaaaaa attgaaagca tatctacaac gaatgggacg
    11101 ttcccttcaa cttccagttg cggtcttacc tgctgctgct ttactagtag ggatcggaaa
    11161 ctggtgggca tcttatagta acgacattgt cgctcatttt ttacaagctg gcggtaacgc
    11221 agttttaggt caattacctt tgctgtttgc cgttgggtta gcactaggga tgtcagtgga
    11281 caaatcaggt gctgctgcct tggcaggttt ggtcgcattt gaagttccag taaatgtttt
    11341 aaaaactgat tcagtagcta ctttattgaa tatcaaacca gaatcagttg atccatcttt
    11401 tgctcagatc agtaacgtat ttattggtat tattgccgga ttgattgcgg cagctcttta
    11461 taatcgtttc cacgaagcaa aattaccgat ggctttatct ttctttagcg gcaagcgtct
    11521 agtgccaatc ttggctgcct ttgtcatgtt gctgatcagt gcagtgctac tgctcgtatg
    11581 gcctccggtt tactctgctt tagtttcttt tgctaaattc attttgagtt taggctgggg
    11641 tggtgcaggt ctttatggct tcttcaaccg tttactgatc cctacaggat tgcatcaagc
    11701 aatgaactcc gtgttctggt ttgacgtagc aggaatcaat gatattggta aatttttggc
    11761 ttctgaagga acaaaaggcg taacaggaat gtatcaagct ggatttttcc caatcatgat
    11821 gttcgggctt ccagcaggag catatgctat ttacagaaat gcacgtccag aaaacaaagc
    11881 aaaaacagct tccttgatgc tagcagcagc ttttgcttca ttctttactg gtgtgacaga
    11941 accacttgaa ttttcattta tgttcgttgc ttggccatta tatgtcttgc atgcaatttt
    12001 cactgggatt tctttagcag tgagtgcatt cttccattgg acagcaggat ttgcctttag
    12061 tgccggattt gtcgattact tcctatcttt gaaaaatccg attgcgaatc agccattgat
    12121 gttgatcgtc caaggattag taacagcagt gatttactac tttggcttta actttgctat
    12181 caaaaaattc catctgatga ctcctggccg cgaggaagca gatctttcag atgacgatac
    12241 tgcaacgact aattcagaca ataaatatgc ggcacaagca agcaaaatct acgctgcatt
    12301 aggcggagcc gacaatgtaa cttcgatcga taactgtact acacgccttc gtttgcaagt
    12361 caaagataca ggcctgatcg atcaaaataa aatcaaagcc actggcgtac ctggtatgaa
    12421 gatcatcgac ggaaaaaatg cgcaagtcat cgtaggtaca gaagtacaat tcgttgcaga
    12481 tgagatggca aaactacatg gtggtgctgc agcacgacct gcacaaacaa acaccgtggt
    12541 taaaacagaa acatttgccg gtgaaacgat cgaacaagat atttacgcta tcgcaaatgg
    12601 gaaactgatt ccaatcactg aagtttcaga tgatgtattc gcggaaaaaa tgatgggtga
    12661 cggctatgct gtattaccag aaaatggaga aatcttctct ccaatcgctg gtacgatcac
    12721 aaacattttc ccaacgaaac atgctgtcgg aatccaaaca gatgctggta tcgaagtctt
    12781 gcttcatatg ggcatcaaca ctgttgactt aaaaggcgaa ccattcactt tatatgtaga
    12841 agaaggtcaa aaagtcgcaa gaggtcaatt gattgcttta gttgatcttg ccgctattca
    12901 atctgcaggc aaaaacacgg atatggtcgt tgtattcaca aatggcgaca aagtccaaag
    12961 cttagaaatc gaacccgcac gtgatgtaaa agcaaatgat aaaatcggct ctgtttccaa
    13021 caaagcctga gccatcgtct tccccagact tttttatcga taaacaaaca ggagctgaga
    13081 caaaaatctt gtctcagctc ctttttccga ataaaaacgg cgttctcctg ccaactccgc
    13141 ggtaataagt ccagctaaac gaaacatatg aagagctatt ttttatttga ctcttcttat
    13201 tacttgatgc agaacagctt tttcacaaaa tttttcttca tactcaaaac taatcggctt
    13261 tttcacaacc tctttataca ttattcctga tccaatattt cttttaatgc tgcttcaaga
    13321 gaagttaatg gttttcccaa tactttttct aaatcatcag aggaaatagc taactgatcc
    13381 tttttaatat catgttggat cgctaaaaac atgtcagctg cttcttgagg gaatccggct
    13441 tcttttagat tttcgacaaa cgctgcatca cttgcttcaa taatttctaa ctctttacct
    13501 gtcgcacgct ctaatgcctt ggctaattct tcatagtcag ttagattgcc ggataattct
    13561 aagatttctg gagagtcact ttctactagt acttttgctg cagcttctgc atattcacgt
    13621 ttcaatgccc aacctgtttt tccatttcct gcagcataaa cgaattgacc agtttttaaa
    13681 gcctgcccaa tgataggtaa ttcattctct acataccaat tattccgtaa aaatgtatgg
    13741 gaaatacctg aatccttaat cattttttca gtgaattggt gatctggcgc taatacactg
    13801 atcgagtgat cggcatctgc aaaacttgta taagcgatat aagatacccc agctgcttgt
    13861 gctgccttca ctacattctt atgttcggct tgacgatttc ctggaacgcc agagacaaaa
    13921 agcaatcgat ctattccttt caacgcttct ttcattgagt ctaaatcatc ataatcacca
    13981 atacgtatat taaatccagc ttctttcagt gcttctcctt tttcttcact acgtactagt
    14041 acatagattt cattttgtgg aaccatcttt tttaagaatt ccagcgcata accaccaaaa
    14101 cctccagttg ctcctgttac taaataatgc atatttgcca ctccctattc tttttatttg
    14161 ttaagattta cagctgtaca tttataaatt acacctttat cataaagagt gtggtaagat
    14221 aagtcaagca aaaaaaatga aaggatgaca actgatgaag tattctctgc aatttagtga
    14281 tgcaatccat gtacttgcct atattgaaat gtttcaagat acccgatcac tttctagtga
    14341 gatgattgcc agtagtatcg aaacaaatgc agcaaatgta cgtaaaatca tgagtcagct
    14401 caaaaaatcc ggtttgatct ttacaaaaac cggaaaaccg cagccttccc ttgccaaaag
    14461 tccagatcag atttctttac tagatgtcta tcgcagcatc gaaggtaata cacagcttat
    14521 ccaagtcgat ccaaaaacaa atccaaattg cgtcgttggt gcgaatatcc aagaagcttt
    14581 agaagaaagc tacgctcggc tgcagaaaaa ggcagaagaa gaaatggcta agatcacact
    14641 tgatagtctg atacataaaa tagccgtttt ggaaaaagaa aaaagaccag aaaacacagg
    14701 cctcgtagag agatttttat aattctctct actgtgcctg catttttctg atcggaatcg
    14761 tctacataaa ttcttttatt ctccggggga caaaagcctt gtcatgcccc tttttcctct
    14821 ctaatgctca ggtcaaagcg ctgaagtcat gacgtcttga taaacggtgt tcaagtagct
    14881 gaccctcggc attagttctg aaaaatgcaa aatatggaaa gctatttccc atttttcctc
    14941 tctaatgctc aggtcaaagc gctactttca cgacctctat attatcatgg tttattgact
    15001 accggtatcc agatttctcc catatatttc ccgttggtaa ctcccatttc gactacagca
    15061 tttggacccc caacataggc gtaatcttct acttctctta aaacatgccc aaaggccgta
    15121 ttcgtcaaat catttcttaa ttcttcaggc gattctgcta aagcgcttac aacaagatac
    15181 gttccttttg ggaatgggat gattcgcgta gtgtttggaa catcctgact cgtttgaaca
    15241 ccgacatagt acatcatttt cccgttatag ggttcgttga ctgtaaagaa ataatgattt
    15301 tcagcagctt gtttcaatgc atctattgtt ccattaccga taactttttg ctcaaatgct
    15361 tgtttctctg cagccaaacc gacaaaatcc gtattgctac tcttcaattc cacaccaata
    15421 cccgtcaccg taaaatctgg tttttcttcg atcctatact ttgccatttc tcccaccctt
    15481 tctattatat taagttcatc ttacataaaa ttaaagggaa atgacacgat tacgctcttt
    15541 tttctaggga tatttttatt ttttaactat ttttttgctt tcactgcaat ccaaatctca
    15601 ctgtaaacat tactgaaatc ctctccccat tttgtaaaag agatctcggg agcttcgact
    15661 aacccataat ctgaagaagg aagccattct gcatagattt tcccccaagt atcttgtaaa
    15721 gtttggggaa atggcccttt attaggaaaa atggcccaag tgtgttcttc tatagcaagc
    15781 atttctaaat cagcgtacgg attttctttt gtcgtagcaa agccaatcat gtgcatcaaa
    15841 ctgccctttt cttccaagcg gtctccatca aatccataag aaacgttgat tacttgattt
    15901 ggatataaat ctcctaactt atgcatctct tccctttgag tctccgtgat cgtctgcgct
    15961 aacttttgta tttcttgatt gactccttca aatttgacag gaactttttt cgccacacct
    16021 gccacataaa actgttcttt cttttcgata cgaaattcca tcgattttcc tcctctgaca
    16081 tcaataaaaa atgtcaagcg tggaaaagat ttttgtatgc cgtatctgct gatttctgaa
    16141 ggcagatatc ctgaccaatc acgaaaagct cttgaaaaac cttcgactgt ttcatatcca
    16201 tatctaaacg ccgtttctgt cactcttgat ccatctagaa gatcgcgatt agccaaagcc
    16261 aatctacggt ttttgatata gtccattagc gacatccctg caataaacgt aaagatgcgt
    16321 tttaattgat attcagagat tcctgtattt tcggctatta ttttatagga aagtccttct
    16381 gtcaaatgtt cttcgatgta atcgatcaat tggttcagat ctttaagcat atttatcttc
    16441 ctccttgatt gcctttttat catattatca aagaaagtca attacttgat gaatcaggaa
    16501 ccaatttagt cagatgtaga aaatgttgta tggatctaaa gaaaattctc aagcagcgtt
    16561 tcgtaacgga taattggtat tatctgccta tctatgtttt acctttagaa aaataaattt
    16621 tggggaagac agattctaag taaacaaagc ggtaaaaata tcttttattc ccttagttaa
    16681 tccgcttact acctactgta caaagcaaat tgttgttcaa actgctgatc agaacgctgc
    16741 ttggattttt cttt
//
LOCUS       DANKHL010000050        16388 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000050 DANKHL010000000
VERSION     DANKHL010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.052"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..1710
                     /locus_tag="PW101_001137"
     CDS             <1..1710
                     /locus_tag="PW101_001137"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDL2513549.1"
                     /translation="YAGMERFAARKAIVQDLKEIGRLIKIDTMIHSVGHSERTGVVVE
                     PRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFNQTFLRWMENVHDWVISRQLWWG
                     HQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDTWFSSALWPFSTMGWPDTESEDY
                     KRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPFENVLIHGLIRDEQGRKMSKSLG
                     NGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDASWNFINKIWNASRFVIMN
                     IEGMTVEEIDLSGEKSVADRWILTRLNETIERVTELFDRFEFGEAGRQLYNFIWDDFC
                     DWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRLLHPIMPFVTEEIWEHIPHQGNS
                     LVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIRSEVNTPLSKPITLLIKTNDAKI
                     DQFLVENTSYIERFCNPEELTISSEITAPDLAMSAVLTGAEIFLPLAGLINIEEEIKR
                     LEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAERAKEKDYLEKQTAVKERIESLR
                     TIQ"
     gene            2020..3333
                     /locus_tag="PW101_001138"
     CDS             2020..3333
                     /locus_tag="PW101_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDL2513550.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            3323..3976
                     /locus_tag="PW101_001139"
     CDS             3323..3976
                     /locus_tag="PW101_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2513551.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            4031..4723
                     /gene="radC"
                     /locus_tag="PW101_001140"
     CDS             4031..4723
                     /gene="radC"
                     /locus_tag="PW101_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDL2513552.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            4873..5073
                     /locus_tag="PW101_001141"
     CDS             4873..5073
                     /locus_tag="PW101_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2513553.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(5327..7006)
                     /locus_tag="PW101_001142"
     CDS             complement(5327..7006)
                     /locus_tag="PW101_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL2513554.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(7008..7220)
                     /locus_tag="PW101_001143"
     CDS             complement(7008..7220)
                     /locus_tag="PW101_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDL2513555.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            7613..9343
                     /gene="efrA"
                     /locus_tag="PW101_001144"
     CDS             7613..9343
                     /gene="efrA"
                     /locus_tag="PW101_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HDL2513556.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9340..11100
                     /gene="efrB"
                     /locus_tag="PW101_001145"
     CDS             9340..11100
                     /gene="efrB"
                     /locus_tag="PW101_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HDL2513557.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            11195..11392
                     /locus_tag="PW101_001146"
     CDS             11195..11392
                     /locus_tag="PW101_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513558.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            11545..12030
                     /locus_tag="PW101_001147"
     CDS             11545..12030
                     /locus_tag="PW101_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDL2513559.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(12134..12727)
                     /gene="clpP"
                     /locus_tag="PW101_001148"
     CDS             complement(12134..12727)
                     /gene="clpP"
                     /locus_tag="PW101_001148"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDL2513560.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            12907..13434
                     /locus_tag="PW101_001149"
     CDS             12907..13434
                     /locus_tag="PW101_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513561.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            13525..14262
                     /locus_tag="PW101_001150"
     CDS             13525..14262
                     /locus_tag="PW101_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL2513562.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            14266..15183
                     /gene="miaA"
                     /locus_tag="PW101_001151"
     CDS             14266..15183
                     /gene="miaA"
                     /locus_tag="PW101_001151"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDL2513563.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            15185..>16388
                     /gene="hflX"
                     /locus_tag="PW101_001152"
     CDS             15185..>16388
                     /gene="hflX"
                     /locus_tag="PW101_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDL2513564.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTN"
BASE COUNT         5262 a         2838 c         3576 g         4712 t
ORIGIN      
        1 tatgctggaa tggaacgttt tgctgctcgt aaagcaatcg tgcaagatct gaaagaaatc
       61 ggtcgattga ttaaaatcga tacgatgatc cacagtgtgg gtcattccga gcggacaggt
      121 gtcgttgttg aacctcggct gtctactcaa tggtttgtaa aaatggcgcc gttagcgaaa
      181 aaagccattg ataaccaaga aacagatcaa gcagtggaat tcttccctcc aagattcaat
      241 caaacgtttt tacgttggat ggaaaatgtc catgattggg taatctcacg acaattatgg
      301 tggggacatc aaattcctgc atggtatcac aaagaaacag gtgaaatgta tgtagggatg
      361 gaagcaccag cggatagtga aaactgggta caagatcctg atgttttaga cacttggttt
      421 agttcagctt tgtggccttt ctcaacaatg ggctggccag atacggaaag cgaagattat
      481 aagcgatact tccctactag cactttagta accggttatg acatcatttt cttctgggta
      541 agcagaatga ttttccaaag cttggaattt actggcgaac gtccatttga gaatgtcttg
      601 attcatggat tgattcgtga cgaacaaggg cggaagatga gtaaatcatt aggtaatgga
      661 atcgatccga tggaagtaat cgaaaagtat ggagcagatg ctctccgctg gttcttatcg
      721 aatggttctg caccaggaca agatgtccgc ttcagctatg aaaaaatgga tgcttcttgg
      781 aactttatca acaaaatctg gaatgcttca cgtttcgtta tcatgaatat cgaaggaatg
      841 accgtagaag agatcgattt atctggagaa aaatcagtgg ctgaccggtg gatccttact
      901 cgtttgaatg aaacgatcga acgagtaact gagctatttg atcgttttga atttggtgaa
      961 gcaggtcgac aactgtataa ctttatctgg gatgattttt gtgactggta catcgaaatg
     1021 agtaaagaag tactttatgg agatgatgct gtttcaaaac aaaccacccg tagcatctta
     1081 gtccatacac ttgatcagat cctacgttta ttgcatccaa tcatgccatt cgttacagaa
     1141 gaaatatggg aacatattcc tcatcaggga aattctctag tagtggcaga atatcctgtt
     1201 gtacgtccag agtttgatga tgaaacagct tctaaaggga tggaagtatt gaaagaattg
     1261 attcgagccg ttcgaaatat tcgttcagaa gtgaatacac cattgtctaa accaattacg
     1321 ttgctaatta aaacaaacga tgctaaaatc gatcaatttt tagttgaaaa tacaagctat
     1381 atcgaacgct tctgtaatcc cgaagagttg acgatatcaa gtgagatcac tgcgccagat
     1441 ctagcgatgt cagctgttct gacaggtgca gaaatcttct tgccgttagc aggattgatc
     1501 aatattgaag aagaaatcaa acgattagaa aaagaactgg aaaagtggac aagcgaagta
     1561 aaacgtgtcc aaggaaaact ttctaatgaa cgttttgttt ctaacgcgcc ggaagaagta
     1621 gtagaggcag agcgtgcaaa agaaaaagat tatttagaga agcaaacggc tgtcaaagaa
     1681 cgaatcgaaa gtttgcgtac tattcaataa gaatagaaaa agagagtgtg aaatcagcta
     1741 gctattacta aaagctagct gattgaacat cgttacagga aagagtttga gcctcactct
     1801 tagcttcgat tcataaggag atgtgacata aattttgtcg catctctttt ttagtctact
     1861 cttattatgt aggagaagga gggaggtgga tcatgtatca agagtcaata aagtagttga
     1921 taaaatcgtt cctctctctg tttcttttct ctttgtcata cttttatttt tcttatgtgt
     1981 tctatttgtt ataataaaag agaaatgggg gaattgttga tgacgattga agaagcaata
     2041 gaatggatcc atagtcgatt accatttggt tcacgtcctg gccttgaccg agtcgaagct
     2101 ttattagaag tggtagggca tccagagaga aaagtaaaaa cgatccatat cgctggaaca
     2161 aacggaaaag gttctactgt agcttatttg agaagcctat tagaagaggc tgggctgaca
     2221 gtaggtacat ttacttcacc ttatatcgaa acgttcaatg aacggatcgc aatcaatggc
     2281 aaaagcattc cagacaatga tttgatccag cttgtaaaaa aatatcagcc actgatcaaa
     2341 caacttgatc aatttgaagc agtagctggt attactgaat tcgagacatt aacagcaatg
     2401 gcatttgatt attttttgat gaagaaagtg gacgtagcga ttatcgaagt tggcttaggc
     2461 ggcttattgg attctaccaa tgtagctgta ccaatgctga cgggaataac aacgattgga
     2521 cttgatcata ccgaaatact tggagataca attgaagaaa tcgctgcaca aaaagctgga
     2581 atcatcaaac aaggcgttcc agtagtaaca ggaaatattg ccccagaagc ttttactgtc
     2641 atccaacaaa cagctaaagc aaaaaatgca ccgctttatg tatacgggga aaattataaa
     2701 ataaactatc tgcatcctga tgagatgtgg ggagaagtat ttgaattttt taatgaaata
     2761 ggaaagatcc ctcaattaac cactccgctt ttagggcaac atcaagctga aaatgcagga
     2821 gtagccatcg aattgtattt tgtttactgt caattggaaa agctgccttt ccgagaaaaa
     2881 gatgttttga agggattgaa gaaatctcga tggccaggaa gaatggagcg tttgagtaac
     2941 gagccattag ttgttttaga tggtgcccat aatacgcatg caatgaaccg gcttgttgag
     3001 aatttgaaaa aagaattcaa aggatatcaa atcaacattt tgttttccgc cttacgaact
     3061 aaaaatgtta gagaaatgct agaacgattg cagcaagtac caaatgtcca tatttatttg
     3121 acgacctttg aacatccaaa agccatcgaa ttaagtgaag tatcagattt agcaagtgag
     3181 agagtatcaa tcgtttcatt gtggcaattc ggattagcag atatattgga aaagatgaca
     3241 aatgaagacg tattagttgt gaccggttca ctttatttca tttcagaagt tcgccattta
     3301 ttaatacaat taggaggaga tcatgaagta taatgcagtg atttttgaca tggatggttt
     3361 gctgttcgac acagagttag tgtattacga agccacacaa gtagtagcag acaagatggg
     3421 ttttccttat gataaagacc tgtatttaaa attcttaggt gtttcagatg aagaagtatg
     3481 ggcgaattat catatgattt tctcggaatt tggcaaaaca agtgtacaaa gattcataga
     3541 tgattcatac caggaaacgc ttgaacgttt ttcgcaagga gcggtacaaa tgaagcccgg
     3601 tgtgttggaa ctcctctctt ttttagaaga acatcagatt cccaaagttg tcgcttccag
     3661 taaccaacga gcagtcattg aacttttatt gaagaagaac aatctgtttg aacgatttga
     3721 gacgattgtt tctgcagaaa atgttcaacg agcaaaacca gacccagaaa ttttcttgtt
     3781 agctaatcag aagctaggta cagcgaagaa agaaacattg attttagaag attcacaaaa
     3841 tggtatttta gcagcttctg cggctgagat cccggtagtc atggtaccag atctgcttcc
     3901 accatcccaa gaattgatca aaaaaacagc tggggttgtt tcttctttac atgaaatccc
     3961 tgcattgtta aaataaaaat ccatctcttt tcgtatctta ttttagataa gatagaaagg
     4021 agatgttttt ttgggaaaac gattgatcaa agaagtacct actagctctt taccaaggga
     4081 acgtatggaa atttatggcg cggaagcttt atcggatcag gagcttttgg caatcttact
     4141 gcgtaccggt caacaccctt atagtgtgat gtcgattgct ggaaatctgt taaaaacgtt
     4201 tggaggactg gcctctttac gacaggctac tttgcatgag ttagaagaga ttcgagggat
     4261 aggacgcgtg aaagcaatcg aaatcaaagc cttgatcgaa ttaggtcgac gaattcagac
     4321 ggatcatgaa agagcggctc caccggttcg taccagctac gaattggccc agcaattgat
     4381 tctggaaatg aaagatcaca aacaagaaca tttggtttgt atttatttgg acacgaagaa
     4441 tcaagtgatc ttaaaaaaaa cagtgttcat cggttcgctg aatcagtcag tcgctcatcc
     4501 tagagaaatt ttccattatg ctgtacgtta ttgtgcagca aggatcgttt tagcgcataa
     4561 tcatccttct ggtaatgtta ttccatcgca gcaggatatg aattttacca aaaggataca
     4621 aaagtgtgga gaaatgatgg ggatcactgt actggaccac ttgatcatcg gacgcaaacg
     4681 atattttagt ttgagagaag aaggaatgat ggaagagaag taagttgctt tcttgcaaaa
     4741 aaaatgaagc aagtgtacaa ttatattgta attttgtttg ggaatagaag ttgaaacatc
     4801 tgttgcacct ctatcgtaca cgagcaatgt tacaatttaa attagtgctt ttggcacgag
     4861 gaggatatta ttatggaaaa cggaacagta aaatggttca acgctgaaaa aggatttgga
     4921 tttatctcac gtgaagatgg aagcgacgta ttcgttcact tctcagctat ccaaggtgac
     4981 ggattcaaaa cattagaaga aggtcaagca gtgactttcg acgttgaaga atcagaccgc
     5041 ggcccacaag ctgcaaacgt tgtaaaagct taattttatc tgtaaatcaa attgatggta
     5101 gacatcaatt tttacgagag aacaaagcga gtaacaaaaa tacctgccac aagagacaat
     5161 ttctcttgtg gcaggtattt ttttgtatga aaaagtaaat aaatttaaga ggctatgaaa
     5221 gagccacctt gtatcaagga ataagaacaa acaaaaatgg cttctcatgt ttttcatttg
     5281 ttttctctta tattcaggac aaggtgctga tttcataacc tcttatttat tcttcatctg
     5341 gtgtcattac cattggcaaa atcattggat gtctttctgt acgttcgaat aagaaaggct
     5401 gaagcgcgcc agtcatcgct tctttcaatt tatattctga acagtcaggt ccattcaaac
     5461 attctcttaa agcgtggaat aagattcgct gagcttcatt gatcatttct cctgattctc
     5521 gcatgtagac aaatccacga gagagaatat ctggaccagc caagatttct tttttcttga
     5581 tatccactgt cgcgacagct aatacaagac cttcttctga aagcaggtga cggtcacgta
     5641 aaacgacatt accaatatca ccgatcccgc taccatcgat atacacatct ccagccacaa
     5701 aatgcccagc aggacgcgca gaatcgctag ttaacgctaa aacatcgcca ttttccatga
     5761 tgaagcagtt ttctgcagga acacctacat cttgtgctag agaagcatgt actttcaaca
     5821 tgcggaattc tccgtggaca ggaataaaat attttggctt catcaaacga agcattaatt
     5881 tttgttcttc ttgtccaccg tgaccggaag tatggatgtt attgattttt ccgtgaatca
     5941 cttctgcacc agcttcagac agcaaattga tcaaatgatt gacacttgtt gtattaccag
     6001 gaataggtga acttgaaaag atcactgtat cgtctggctg gatttggatt tgacggtgag
     6061 taccattcgc gatgcggctc aacgcggcca tcgcttctcc ttgagaacct gtacatagga
     6121 tcatcgtttc attggcaggc aagcgattga tttcgtgtgc atctacaaat gtgccgtctg
     6181 gaactttgat atatcctaaa cgttgaccgt tgacgatggc attttccatg cttcgtccga
     6241 aaacagcgat tttacgacca gttgccacag cggcatctgc tgcttgctgc aaacggaaga
     6301 tgttagaagc aaagctggca aaaatgatac gtccttcgat ttttgaaaag atcttcaaaa
     6361 tagaatgccc aatcgttttt tctgatttag taaatgttgg aatctcagca tttgtactat
     6421 cagataaaag gcacaagact ccttcttccc cgatacgagc cattttatgc aagtttgctg
     6481 gttcgccaac aggagtgaaa tcgaatttga agtctccagt cgctacgata tttccagaag
     6541 gtgttttcac cacgatccct aatgcatctg ggatactatg ggtcgttctg aaaaagctga
     6601 cagaagtctt tctaaaacgg atgatcgtat cttcattgat ctcatggagc tctgcatctc
     6661 gaagcaagcc atgctcatct aatttgtttg tgatcaacgc taatgcgaga ggaccagcat
     6721 agataggaat atttgcttgt ttcagcaagt agggtattcc gccgatgtgg tcttcgtgcc
     6781 cgtgagtaat caccaaggct tttactttgt gtaagttttg tatgatgtag ctgtagtcgg
     6841 gaatgacata gtcaattcca agtaaatcgt cttccgggaa tttgatcccg gcatcaatca
     6901 aaatgatttc atcttggaat tgtaccccat acgtattttt cccgatttca cctaatccac
     6961 caatagcaaa gacgcctgtc tcgttatttt tgatggaaag tttcatatta aaactccact
     7021 aatgagaaat cagcattttc tttttcgtac tctagatgac ttccttctag aagttgaacg
     7081 aattcaatat tgtatggggt attttcttcc acacgttggc gagcgtctac atcgcttttt
     7141 gcctcaatgt aaagagattt tgtttcttcc cgtttagggt tgcgtacttt tgtttcttga
     7201 taataaactt tatagatcat tgttttgtct cctttatctg cgtaaagcag tgatttagct
     7261 agttgatttt tttagacaac acaaaacgag gggacaagcc acctggtttt tcatatgctt
     7321 tgactagaat actggattcg agtcaaagag acaagcctcg tttttgctta tctgttcgga
     7381 tattttttca accgtacctt ctgtataaaa aatatacaac aattaattgt tattctagca
     7441 taaaacagca aataagtata gaaatcaact agattgaaag aaaaaatcaa tgtttatcat
     7501 cccgctataa tcataatgac ttttttatga gagaagtgtt ctattaatag gctcgttgtg
     7561 ctatcatttt tagtaatgaa aatgtaacaa aagattaaaa ggagatgaga aagtgaaact
     7621 aatgtggcat tataccatgc gttataagaa attcttgttt ttcaatttca tatgcgtatt
     7681 tggttttatt ttgattgaat taggacttcc tacgatttta gcacgtatga tcgatgtagg
     7741 aatccgcaat gatgattttg attatgtgaa gcaacaaggg atatttatga tcgttattac
     7801 tgtaatcggt attattttga atatcttgtt aggttatttt ggttcgagga ttacgactaa
     7861 tatcgtagca gatattcgag atgatttgtt cgaaaagatc caaagttatt cgcatcagga
     7921 atacgaaaat ctaggcgtat cttcattgat cactcgtaca acaaacgatg cttatcagat
     7981 catgttgttc ctgcaaaata ttttacgtat cggttttatg gcaccaatga tgttcattgt
     8041 cagtttgtac atggtcatgc gtacaagtgt gacactcagt ttgtatgtag taggtgcatt
     8101 acctttacta ttattggcag tcgtagcgat tgctaaggtg tctgaaccgt tatctaaaaa
     8161 gcaacaaaaa aacttagata aaatcaacag tatcttgcgg gaaaatcttt caggattgcg
     8221 tgtgattcgt gcattcgtca atgaaaagtt tgaagaaaaa cgttttgctg tagtcaatga
     8281 gaattatgct tctagttcaa aaagcttatt ccgtttgatg gctgttgcac aaccgggttt
     8341 ttatttctta tttaatatcg taatggtatt gatcatctgg agcggggcag tgcaaatcga
     8401 tgcaggcgag cttcgtgtag gagatttgat tgcgtttatc gagtatatat tccatgcctt
     8461 gttctcattc atgttatttg ctagtgtgtt catgatgtat ccaagagcag ctgtttcagc
     8521 tggacgtatc caagaagccc tagatgccaa accttcgatt gtagaagatc caaatggtat
     8581 atctgataca aaaactaaag ggtatatcga atttcaaaat gtaacatttg cttatccagg
     8641 ccattcgcaa gagccagtga tacgaaatgt cagttttaca gcctctccag gagagaccgt
     8701 tgcttttatt ggaagtaccg gtagcggaaa atctacgttg atccaattga tccctcgctt
     8761 ttatgatgtg agtgaaggaa agatcttgat cgatggagta gacgtgagag attatcggct
     8821 aagcaagttg cgagataaga ttgggtttat tccgcaaaaa gcgctgctat ttactggaac
     8881 gattgcagat aatttgcgat acgggaaaga agatgctacg caagaagaaa tggaacgtgc
     8941 agctgagatt gctcaagctt cagaattcat ttctcgaaaa ccagatggat ttaatgaaca
     9001 tttatcagaa ggtggttcca atttttcagg tggacaaaaa caacgattgg caatagccag
     9061 agccatcata cgccgtccgg aaatctatat ttttgatgac agcttttctg cattagatta
     9121 tcaaacggat gcgaagttac gtgctcgtct gaaaaaagaa acgacagaat ctactgtatt
     9181 gatcgtagca caacgtgtgg gaacgatcat gcatgcagat aagatcatcg tattaaatga
     9241 aggagaagtc gtcggtatgg ggacgcatcg ggagttgttg gaaaaatgcc cgatttatta
     9301 tgatattgcc gcttctcaat tatcaaagga ggaattagca tgaaaaaaac cttttcttcc
     9361 ttaaaacgtt tagctcgata tatcagaccg tatcgtgaga cttttctttt agtgatccta
     9421 tttacagttc tgacagtggc tttcaacgtg gctttgccat atgttacagg acttcctact
     9481 acagaaatca gccgtaatat tgccaatggc gaatcaatca atttttctta tatattcaaa
     9541 tgtctcattt ggatcacagt tgttggaata gggtattgta tttcgcaatt actttctggt
     9601 gttttgatga caaatgtcgt tcaaagtgcc atgcatgatt tgagaagaga catagaagaa
     9661 aaaatcaatc gtcttccggt ttcatatttc gataaaaacc agcaaggaaa tattttgtct
     9721 cgtgtgacga atgatgtaga tgcagtcagc ggtgctttac aacaagcttt tattggtatc
     9781 gtaaatgcga tattgggaat tacgttggct gcagcaatga tgttttatat ccagccgatg
     9841 atggcgctga tctctatgat catgattcca acatcgatct ggatctcaaa acgagtgatc
     9901 aatgcttcgc agaaatattt tcaaagcatg cagaattctc ttggtgagct aaacggctac
     9961 gtccaagaaa atatgacagg ttttagtgta ttgaaagtat atggacgcga aaaagaaaca
    10021 ttggaaggat tcaaaaaagt taatcacagc ttgaaatact ttgggtttcg tgcagcattc
    10081 atttctggct tgatgatgcc tctagttcaa ttaactgcct atgctactta tattggtatg
    10141 gctgttctag gaagctttta tgttattact ggggcgatcg ttgtcggtca actacaagca
    10201 ttcatccaat atatttggca aatcagtcag ccaatgggga acgtgacaca attgtcttca
    10261 gttttgcaaa gtgcatcagc ggcaacgaaa cgtgtattcg agattttaga tgagccagaa
    10321 gaaaaagaaa acaaagtaga tgcttcactg ccagaaacga ttttaggaga tgtgacattc
    10381 gatcatgtcg attttgcata tgatccgaag aaaccattaa tccaagattt gaattttgaa
    10441 gtgaaagctg gtcaaaccgt agctgtggtc ggtccgaccg gtgccggcaa gacgacattg
    10501 atcaatctat tgatgcgttt ttatgacgta gacaaaggag caataaggat tgatggaatc
    10561 gacaccaaat caatgagtcg cagtgatgtg cgttcgctat ttggtatggt cttgcaagat
    10621 gcatggcttt atcaaggaac gattgcagat aatatccgct tcggaaagct tgatgcgaca
    10681 gattatgaag tagttgatgc tgcgaaaaca gcaaacgtag atcattttat ccgaacgatg
    10741 ccagatggat atgatatggc catcaattct gagggagaca atgtgtcttt aggacaaaaa
    10801 cagttgctga cgattgcccg agcagtggtc tccgatccta aaatcttgat tttagatgaa
    10861 gcaacaagtt cagtagacac ccgtttagaa gcgttgattc aaaaagcaat ggatcgcgtg
    10921 atggagggac ggacaagttt cgtcatcgct caccgtttat cgacaatccg tgatgcagat
    10981 ctgattctag tgatggatca aggtcagatc attgaaaaag gaacgcatga gagtttattg
    11041 gcacaaggtg gattttatga aaaactgtat aacagccaat ttgctgagga agcagaataa
    11101 gtcatataag aataaaacag tttaaaactt attttttgtt ctctattctt gataatggta
    11161 aagtaaagtt atcaagaaaa gtgaggacac ggagatggaa cagtttttaa atgaaaaaat
    11221 tactgaagcc aaaagagaag ttgatcgttt aacgcacgtc tgtcagacga agccttttga
    11281 ttcagctgat ttttttgaaa atgagtttca aatcgaacgc ttggcagcac aaatagaagt
    11341 gtacgaatta attttaaaaa aagtaataga agaaaatata gatgtacaat aaaaacaggt
    11401 aaaacgtcag ttaccagata ctgtaaatag acatctggcc tgagcaggtt ttacctgttt
    11461 tttttcttat gaaataaata tatgctatga tacctttatt caataggatt agtttctaca
    11521 aaaaatgaaa ggagaaaaat aaatatggaa cggtttatag catcaaatgc cacagtgatt
    11581 ggtgatgtga cattgtcaga agatgtgacg atctggtatc aagctgtttt acgcggggac
    11641 agtaattgga taaaaatagg acaaagaacg aatatccaag atggtacaat catccatgtc
    11701 gatcatgatg cacctgtaga tattgcggag aatgtgacgg taggacatca gtgcatgcta
    11761 catggctgta caatcgaaaa aggggcgctg atcgggatgg ggaccacgat attgaatcat
    11821 gcggttattg gtgagaatag tttgattggt gctggatcat tagtaacaga aggaaaagtt
    11881 attcctccta atgtcttggc ttttggtcgc ccagctaaag tgatccgtcc tttgacaaaa
    11941 gaagaaatac aaaaaaacaa agaaaacatc cagcattatg tcgagatagg tcggatgcat
    12001 gcgcaaggaa aattcaagga gcaaagatag gcagtagcat ccataaaata aaaaaggatg
    12061 gtcagtttga gttgaccatc cttttttatt tggtttagat aagagagctt aaaaaagctt
    12121 cctttgaaac agattaattt aatgaagaac tgttttccat gacttcatcg atcaatccat
    12181 atgcttttgc ttgttcagct gtcatatagt tgtcacgatc tgtatctttt tcaatcactt
    12241 caagcggttg tccagtgcgt tcagctaaaa ttttgtttaa gcgttcgcgt gtttgcaaaa
    12301 tatggcgtgc cgcaatttcg atttcagttg cttgcccttg agcgccgcca agaggttgat
    12361 ggatcatgat ctcagcattt ggtaaagcaa atcgtttacc ttttgtacca gctgtcagca
    12421 agaagcttcc cattgaagca gccataccca taacaattgt ttggacatct gctttgacaa
    12481 agttcattgt gtcgtaaata gccatacctg ctgtaactga accaccagga gaattgatat
    12541 aaagatagat atctttttca gaatcttgag cgtctaagaa aagcaattgg gcgatgatcg
    12601 aattagcaag atcatctgtt acttggccgc ttaacatgat gatacggtct ttcaataaac
    12661 gtgagtagat atcgtaagct ctttcacctc gtgaagattg ttcaatgact gtaggaatta
    12721 aattcataat agagttcctc cttgatttcg tttagaaata gtgtaccata atttagtttc
    12781 taatacctaa aatgtagtat acatttttgg tcaaaaaagg tcaaataaaa aacttaggca
    12841 aattagtcgc ataaacccgc tttatggtat gataagcaga gaaacaaaac aaggaggaac
    12901 aacagattga tagttggtat tactggcctt atcggagtgc tgacaggtgc tgtagttgtc
    12961 tatatcggca tgatactgca aatgcgtcac ttggaaaata aagaacagcg tagacgtgaa
    13021 ttggaattga aagtcaaaga aatcgagacg ctgaatcaat taaataaaaa gatcaacgag
    13081 atcttgcaaa aacggacaac gctgatggaa gaatatgtca gctttgatgc atttgatgat
    13141 tgttatatca cgattgacga ttttgcgtat ctccaatcat tttcagcgca aaatagtttt
    13201 tatctgccaa attatatttt agacgagcta tttaagaata tctcccatcg aaaagtgatt
    13261 ttatctccag atgaaacaat gaagattggt ggatatgctt ataaaggcgg acgcgtgatt
    13321 ttagagaatt ttacggatag tctgactgaa atgatcaatg aaaaaaaagc tcagttgaag
    13381 actatgacgc gccagccgat tggtttcttc tcgaaagatg atgtttggag ctgatatttt
    13441 gaagcattgc cgttgcacat gagagcgtgg taatgctttc tttttttctt aatgatacaa
    13501 tagaactgaa cggagtgaag aaagatgtta caaatctatg cccatcgcgg aagcagcggc
    13561 acccacccag aaaatacgct tcctgcattt gcagaagctg tacgagtagg tgcagatggt
    13621 atcgaactag atgtccatct aagcaaagat ggttatttga tcgttatgca cgacgaagaa
    13681 gtggaccgga cgactaacgg taaagggctg attcgagaaa aaacattgga agaactcaaa
    13741 aaattgaatg cagggggcaa attcagcaaa gaatttccag cagctaaagt tcctgaattg
    13801 agagaagtgt taggactgct cctccaaaaa aattaccgag ggatgctaat gattgaaatt
    13861 aaaactgatc agtatgaata tcccggtatc gaagaaaaat tagccgaaac attaaaaagt
    13921 agagattggc cttttcaaca tgcatattgc agctttcatt tggattcttt ggaaaaaatc
    13981 gcattactgg aaccggagat acagctggac ttattgatga gtacttctga taaaaagatc
    14041 caactagcag cagaaacccc ttatatagaa ggagtacatc caaaaaatac ttggttgttc
    14101 gaacatccag tccaggcagc tgattttcca aaagcaattc gtgcatggac gattaacgat
    14161 gaagaagaaa tggccaaatg tcaagaactc aaaatagatg gaataataac tgattttccg
    14221 gagaaagctc ttcggtggaa acaatcaacg aaaagtaggt aaaatatgga aaaagtatta
    14281 gtgatcgtcg gtccgacagc agtcggtaaa acagcgctaa gtgtggaact cgcacaacta
    14341 tttggaggag agatcatcag tggtgattca ttgcaaatct ataaaaatct ggatatcggt
    14401 acagcaaaaa tcaaagaaga agagatgaat aatgttccgc atcatttgat tgatgtgatc
    14461 gaacctactc aacgttactc agccgctgat tttcagcatt caggtcgaca attgattacc
    14521 gagattgcga accgagggca cctacctatc atagctggtg gtacgggtct ctatatccaa
    14581 tcccttctat atgattatca gttaggagcg acagaagaag cgaatcctga gattagaaaa
    14641 aagtatgaaa cgttagctga aacaattggt tcccaagcat tatggaatca gttgaaagaa
    14701 aaagacccgg cagctgctga gaaaatccac tggaacaatc aacgcaaagt gataagagct
    14761 ctggaagttt tagagacgac aggacatagt atcacggcac ctaaagaaga gccaaagcaa
    14821 ttgtatgatt acctcatgat tggattagat acgaagcgta gtattcttta tgaacgaatc
    14881 aatcaaagag tcgatttgat gctagcagaa ggtttagtag cagaagcgcg taaggtgtat
    14941 gagcttggag aagtccaggc cagtcaaggt attggatata aagaattttt cccttatttt
    15001 gaagggaaag aaacattgga agaagcaaca gaacaagtca aactacattc gagaagatac
    15061 gcgaaacgtc agttgacgtg gtttcgtaac cgtttggatg cacagtggtt tgatcttata
    15121 agtgagccaa atcaaaaaag tcagattgag caaacaatca aaaaatggct ggaggcggaa
    15181 taaaatggaa gaaaaagtga tcatcgtagg ggtagaaacc gaagcaaacc agcgttactt
    15241 ttcagagtca atggaagagc tagttcagct gacgaatacc gcatcaggag aagtcgtatt
    15301 cacaattaca caaaagagac cacaagtgga tcggcaaacg attatcggta aagggaaatt
    15361 acaagaatta gttcaacaag cggatgcgca tgaagcggat ttgatcatct ttaatcatga
    15421 gctaactccg agacagagcc aactgattac cgatgctgtt ggtcttccgg taattgatcg
    15481 cgtgcagctt atcttagata tttttgcgat gcgtgctcgt tcgaaagaag ggaagctgca
    15541 ggttgaactt gctcagttag aatacctgct gcctcgttta gtagggcaag ggaaaacatt
    15601 atctcgtctt ggcggtggga tcggaacaag agggccaggt gaaacaaagt tagaaacaga
    15661 ccgccgacat atacggaata aaatttttgc tgttaagaaa gaattgaaag aagtagaagc
    15721 tcatcgagag cgcaatcgcc aaaaaagaaa aaatagcgag atttttcaga ttggacttat
    15781 tggctacacg aatgccggga agtcaactat cttgaatcta ttgacacaag cggatactta
    15841 ttcgaaagat caattgtttg caacgttaga tccattgact aagagatggc gttttgctga
    15901 aggatttgag attactgtaa cggataccgt cgggttcatc caagacctgc ctacgcaact
    15961 gatcgatgct ttccattcta cattagaaga aagtcaaaat atggatcttt tgctacatgt
    16021 agtcgatgct agttctccag atcgtatttt acaggagcag actgtcttaa aattgatgga
    16081 tgaactgaat atgaaagaaa tgccgatact gactgtttac aataaggcag accagatcga
    16141 cccagcgatg ttcacgccta cgctattccc aaatgtattg atttctactc agacgaaaga
    16201 aggtaagatg gcgttgatcg aagcaatcaa acgtcagctg atggaactaa tgacccctta
    16261 tacactttct gtgccaagca acgaaggaca gaaattaagc gagttgagaa gacaaacaat
    16321 ggttttggac gaagcgtaca tggaagaaac aaacgaatat gaagttaaag ggtttgcaaa
    16381 aaccaatt
//
LOCUS       DANKHL010000051        16289 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000051 DANKHL010000000
VERSION     DANKHL010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.053"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..622)
                     /locus_tag="PW101_001153"
     CDS             complement(<1..622)
                     /locus_tag="PW101_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513565.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPE"
     gene            complement(801..1520)
                     /locus_tag="PW101_001154"
     CDS             complement(801..1520)
                     /locus_tag="PW101_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2513566.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(1651..2802)
                     /locus_tag="PW101_001155"
     CDS             complement(1651..2802)
                     /locus_tag="PW101_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDL2513567.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(3019..3759)
                     /locus_tag="PW101_001156"
     CDS             complement(3019..3759)
                     /locus_tag="PW101_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2513568.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3763..4086)
                     /gene="rsfS"
                     /locus_tag="PW101_001157"
     CDS             complement(3763..4086)
                     /gene="rsfS"
                     /locus_tag="PW101_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDL2513569.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(4143..4736)
                     /gene="yqeK"
                     /locus_tag="PW101_001158"
     CDS             complement(4143..4736)
                     /gene="yqeK"
                     /locus_tag="PW101_001158"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDL2513570.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4720..5370)
                     /locus_tag="PW101_001159"
     CDS             complement(4720..5370)
                     /locus_tag="PW101_001159"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDL2513571.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(5380..5691)
                     /gene="yhbY"
                     /locus_tag="PW101_001160"
     CDS             complement(5380..5691)
                     /gene="yhbY"
                     /locus_tag="PW101_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDL2513572.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(5707..6816)
                     /gene="yqeH"
                     /locus_tag="PW101_001161"
     CDS             complement(5707..6816)
                     /gene="yqeH"
                     /locus_tag="PW101_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDL2513573.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6813..7340)
                     /locus_tag="PW101_001162"
     CDS             complement(6813..7340)
                     /locus_tag="PW101_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDL2513574.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(7520..8305)
                     /locus_tag="PW101_001163"
     CDS             complement(7520..8305)
                     /locus_tag="PW101_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDL2513575.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(8298..9167)
                     /locus_tag="PW101_001164"
     CDS             complement(8298..9167)
                     /locus_tag="PW101_001164"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDL2513576.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(9168..10547)
                     /locus_tag="PW101_001165"
     CDS             complement(9168..10547)
                     /locus_tag="PW101_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDL2513577.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(10555..10974)
                     /gene="fabZ"
                     /locus_tag="PW101_001166"
     CDS             complement(10555..10974)
                     /gene="fabZ"
                     /locus_tag="PW101_001166"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL2513578.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(10999..11475)
                     /gene="accB"
                     /locus_tag="PW101_001167"
     CDS             complement(10999..11475)
                     /gene="accB"
                     /locus_tag="PW101_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDL2513579.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(11478..12713)
                     /gene="fabF"
                     /locus_tag="PW101_001168"
     CDS             complement(11478..12713)
                     /gene="fabF"
                     /locus_tag="PW101_001168"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDL2513580.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12732..13469)
                     /gene="fabG"
                     /locus_tag="PW101_001169"
     CDS             complement(12732..13469)
                     /gene="fabG"
                     /locus_tag="PW101_001169"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDL2513581.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(13481..14398)
                     /gene="fabD"
                     /locus_tag="PW101_001170"
     CDS             complement(13481..14398)
                     /gene="fabD"
                     /locus_tag="PW101_001170"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL2513582.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(14505..14729)
                     /locus_tag="PW101_001171"
     CDS             complement(14505..14729)
                     /locus_tag="PW101_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL2513583.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14782..15744)
                     /locus_tag="PW101_001172"
     CDS             complement(14782..15744)
                     /locus_tag="PW101_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDL2513584.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15754..16194)
                     /locus_tag="PW101_001173"
     CDS             complement(15754..16194)
                     /locus_tag="PW101_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2513585.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         4292 a         3715 c         2788 g         5494 t
ORIGIN      
        1 tttcaggata ttttaatttg acaagcgaaa taatcatttt cagttcttcc atgttttctg
       61 gatattctgg gtcaaatacc ttgctatgac ggccaatccc gccattgtcg atcaatcggt
      121 aaattcgatt ccattcaatg aattggtcaa ttgattggaa attgctgatt ggatagccgc
      181 cacaatccaa aatctctgat acacgaaatg ctaggtatgc actatcgatc actttatttt
      241 ttttcgtgtc tatcttctca tttctttcga taagaatttt ttcaggacga tgtttcaagc
      301 tccactctaa tagtagaaag aaagcctcca tccgatactc ttcttcaaat aaccgaaagg
      361 ctgaatcaga acgacgaaaa agcagcggaa tcagataatt ttgtctataa atcagcattg
      421 gtgcgcggtc caccatacac tctaaataac ggttcagata ccctgtaccg acatagtttc
      481 ctaatcgata gccttcaacg ccgaatcttg cgtatttttg cgctaatttt gaacaatcag
      541 ttacgttcac agctaactct tctactttgc ggtaaccgct taagatacgt tcaaaaggtt
      601 ctaaagtgat ttttgcttcc atattcttcc ccgtccatct tccacatact tcatatcatt
      661 tcacatgata cttatgctct catcatacca caggttgaat tagaaaagaa aataaatcac
      721 acaaaaaagc aagacaatct gttagtgaga ttgtcttgct tttcgaaaaa gactgaatac
      781 ttttcgttaa aactggttgc ttagtcttct aatgccacat tatggaacac atgctgtaca
      841 tcttctagat cttccagtgc atcaatcatt ttttcaaatg tttcttgatc ttctggagat
      901 agtgttacct cgttttgagg gatcatttgg atctcagcga ttgaaaaatc agtgattcct
      961 ttttctttta atgcttcttg tatactatgg aaatccgcta cttcaccgta aacgatgatt
     1021 tgtccttctt cttccattac atcgcgaaca tcgatgtctt tttccattag gtattcaagg
     1081 atctcgtcag cgtcttctcc tgcaaaagca aagattgctg tattgtcaaa catgtaagaa
     1141 accgctccgg aaacacccat gttcccgcca tttttcccaa atgctgcccg tacgtctgct
     1201 gctgttcgat tcacattgtt cgtcaatgta tcaacaatca ccattgagcc gcttggtcca
     1261 aatccttcat agcgcaattc agagtatgtt tcatctcctg caccttttgc tttttcgatt
     1321 gctcggtcaa taatatgttt tggtacatta taagttttag cgcgatcaat gacgaaacgt
     1381 agtttttggt ttgcatgcgg atcaggatca cctgattttg ctgccgcgta gatttcaatc
     1441 ccaaattttg cataaatccg gctattatta gcatctttag cagttttctt tgctacgata
     1501 tttgcccatt tacgtcccat attttcactt cgctttctga tttgatagtt tcataacgca
     1561 aaaacgtcct gttccattat acttgtgaat cacgattctg tgaagctttt tttctttctt
     1621 ccttcattct tctcattttt agtcaattat ctatactcgg attgggattt gcccgaaatt
     1681 ctgttccgga atattgcctc ccaattgata gatttgatca cttctaacta acagttccgc
     1741 ttcttgtgcc tctttttttc cgattcggga ataaatagaa atctcttttt tttcttttaa
     1801 gaatcttctt ccagcatcgg taaatccaag tagtcgaaga taatttttat tttgttggac
     1861 aggaatctct tgctttttga tattcagcaa gacataacaa gataatcgtt ggatccgtgt
     1921 ccatgtatat ctttttgttt ttacctttga cacaaaatcc tcaaaactat cagcagttgc
     1981 agctgctgtt ttcatgcgcg attctattcc ttcggtcatc tgatagatat ctgtcagttc
     2041 cagcaaagta gaagataaca accgataacg caaatacggc caataagatt cccaagtctg
     2101 cagcgaacaa gttgaaagat cttcttctgt ctgaggcggc acaagtccag ctattctttt
     2161 tccttcttct aacgcatggc gaatggctgt tgcactggct atcctctgat ctgtgatgat
     2221 tttttcatga tagcctgcac ttttacgtcg gatcggatgt aattccatag ggtggacata
     2281 cttggcattt tcctttgcat aacttaatcc caagatatga tttggcgaag aaaaatcaag
     2341 cgtaatatcg ggatacagac tgcgaaaaac ctcagtcatt ttttgcggat aactatacgt
     2401 ttgatcagac agttcttgaa atgcctcttc tatcctatct tgattctctt ggacaaagcg
     2461 tccgaaactg ctgtaatcaa acgaagtctc ttcttctgtt ccaaaacaca aactgtcaca
     2521 gtccagtgtt tgcaagattt ttactgcacc tttagcaaaa tagtcagctg actgaaccgc
     2581 ccattcgacg ggaagctcca ctactaaatc cacaccattt tgtaaagctt cattcgcgcg
     2641 atgccattta tcgatgatag ccggttcgcc tctttgcaaa aaattcccgc tcattacagc
     2701 aatgacgacc tctgctcctg ttatttttcg tgccatttcc acatgatagc gatgtccatt
     2761 gtgaaaagga ttgtactcta cgataatccc gcagcttttc atgcgcatcc tccttcatag
     2821 ttatcgataa aaaagaaata gagacaagta aaaaaaggca aaaatcggaa aaaatacccg
     2881 aactatataa actattctcg caaacacaat acttctgcga cgaaaagctg cactgcaaga
     2941 gcaatgccta tatcgttcgg atatataatt tatgttgaag atttattctt ttcttgttaa
     3001 ctcgagcagg caaaaccgct agtctttgta tgctacaaag aaccagcgct ttgatttctc
     3061 tgatgctgct tcgtctgtaa aatcagaaaa tgcttcgact ttcgaaaagc cgctgttttc
     3121 cagcattctt aaataacttt ccaatggata ggtacgctct tgatgtagtt catcttcacg
     3181 aataaatcga ccagaccctt cttgttcttc aacgaaaaag gtaagaaaat gttcgatgct
     3241 gtgtttctcg cttcctggat aactatccca caaaaaagca aaattatccc cttgataatg
     3301 atagctatat tctggaaaaa cctcatctac ttgataggtg gagtgtacgt caaataaaaa
     3361 tcggccattt tcttctaacg cttgatagac ttcatcaaac acttgctgta cctcttgtct
     3421 atttttcata tagcacagcg aatcagaaaa acaggtaatt gcctgatatt gtccgatttc
     3481 tgataaatcg agcatattgc cttccacaaa ttggatcgat gcgtcttctt catatgctcg
     3541 ctggctggca atcatcaaca tctcttctga aaggtctaac cctgtcactt tatatccatc
     3601 ttttgcaaag gcaaccgcta atgctccagt accgcatgcc agttccaaaa tattttttgt
     3661 gtcttcttgt aaatgacgtt tcgaaaaagc taaccattgc tgataaagtg tttcgtccat
     3721 cacttcatcg taaatgaaag caaatgtttc ataagccata tgttaatcca accattcact
     3781 aagatcgact agcggagcat ctgaccatag tttttccaga ttatagaaat ttcgttctgg
     3841 cgcatggaaa acatgtacga tcacatctcc aagatcgatc aatacccatt ttccgccttc
     3901 ttttccttca atacgtttta cttcatattt gttttcttct tctttatcaa tgatttcttc
     3961 tgtaattgcg ttgatttgac gttcgctatt tgctgaacag atcatgaaat aatctgccaa
     4021 caaggaaaca tttcgaacgt ctaaagcgat aatgtcttct gcccgttttg aatctgctgc
     4081 ttcaatcgca atttttaatt gttctttact atctatgatg atttcctcct aatatgagca
     4141 gcttattttt ttgctaccca gtaattatat gtttcgattg tttttggata aatctgttgt
     4201 tcttgcgcaa gcaaatgggc caatgtatgt tttgtttcaa atgctacagc ttcgtctaga
     4261 tcagcccatg caatggctcg tgcatcttgt accccaggaa aatctcgacc tggttcaata
     4321 tagtctgcta cataaatgat tttgtcaagg agactcatct tagcagcccc agtcgtatgc
     4381 acccggattg catgaagaat ctctgcatcg gtgattccta attcccgctc tacaaacgaa
     4441 gcgccaacaa gaccatgcca gattgcatta ttataattca ataactccgg atcaaaaccg
     4501 tctcttacga ttaccatttt gaactcctca tctggtcgtt cttttgcata atcatgtgtc
     4561 aatgcagcaa tactagcttt ttcaggagaa gcaccgtatt ttttggcaag agccacggct
     4621 gtttcttcta ctccaagcac atgcttgaag cgttgttcgc tcattctcat ctgtattttt
     4681 tgcatcaggt tttctctaga ataagcagta tattcattac tatagtccat attcatacaa
     4741 cccttcattt tgaatataat caattacttt atctggaaca aggtagcgga tagaacagcc
     4801 ttctttgatc ttttgacgta ttttcgtgga actgatgtcg atctcaggta catctaccca
     4861 aattacagga tatggagtat ccgttgtgta tcctggtcga cgtataccca caaagttgac
     4921 catagaagtc agctcatcga ttttatacca ttttggcaaa tattctacca tatccccacc
     4981 gataataaag taataatctg tatccggatt gttttgcgtc aattccttca tagtatcata
     5041 ggtgtaactt ttcccaccac gagctaattc aatcgtttca atctgcaaaa aaggattgtc
     5101 ttccaccgct aattccaaca tgttgagtcg atgttttgca tcaatcgtct gtttctcatc
     5161 aacatgaggt ggctgataag aaggcatcaa aaatacgcgg tcaagaccta aattccggcc
     5221 agcttgttca gccatcacta aatgtgcaag gtgaacggga ttgaatgttc cgcccaagat
     5281 ccctacttgc tttttcttct ctaagaattg aggcatttct tcaggaaata attgtgaacg
     5341 aacaaatgtc ttagcttgcg tattcatcat ggtcactcct tatattgctt ttacttcttt
     5401 tgatagcctt tgatactttt cttttgtgga aggtttgtat aatacaataa cacgtccaat
     5461 gatttggacg atttcgcaat ctatttcttt ttctaatact tgagctgctt cttctgcaat
     5521 ctcatctgta ttttgaagta acgatacttt gatcagttct cttttttcta aagcttcttc
     5581 aatttgaacg atcattgctg aattgatccc accttttccg atttgaaaaa tcggctgaag
     5641 atgatgcgct ttgctacgta aaaaacgctt ttgttttcct cttaaactca taattttcct
     5701 tcttccttaa atcaatgctt tgcgtttcaa tacatctaca ccctttggcg cccagcccgc
     5761 aattactact ggcgctgtta cagtgatcca gcctagaccg gcaaaaacga tgtctgtttt
     5821 ttctttcaca gaaaactcaa aacgaacaag ctccggaaat gcctcgactt catcaggacg
     5881 aggaggctgt aataatccac ctacatgttt ttgataaaat tcgtctgctt tttccagttt
     5941 cgttcgatga atatttacat catttgacac ataagcaata aatgaagcac gattgccgct
     6001 gataaaatca aatcttgcaa gtccgcccaa gaaaagtgtt tgcccttcat tcagctgata
     6061 aactttaggt ttgatttctt ttaacggtgc aatgatccgc aaatcttttt tacctaagta
     6121 gtgcgccatt tgatggcgat ggataattcc cggtgtatca atcaaaaaat gcccgtcatc
     6181 taaaggaatc tcgattttat ccaaggtcgt tcctggaaac tgcgaagttg tgattacatc
     6241 ttgtactccc gctgtatttt tgatgatttg attgataagc gtcgattttc cgacatttgt
     6301 tactccgacg atatagacat cccgtccatc gcggtattct tcgatttttt ctaatagttc
     6361 cgtcatctca tgagatttct tagcactagt taataataca tctactggac gaattcctgc
     6421 ttcgtaagca cgttccttca tccattgtgt taatttagtt tttttcaatg atttaggtaa
     6481 tatatccact ttgttcccaa ctaaaagtac tggattgtca ccgacaaaac ggtgtagtcc
     6541 tgggattagt gaaccattga agtcaaaaat atccaccaca ttgacgatca atgcatcttt
     6601 ttgccctaat tcattcagta agcgcaaaaa gtcgtcatcc gtcaatgaga catcttgggt
     6661 ctcattataa tggcgtaatc tgaaacaacg ctgacaatag acttccccgg aagctaaacc
     6721 tttttccaaa gccgtttgcg gcgtgtatcc taattcaccg ggtttgtttg tttgtataac
     6781 ggcaccacag ccgatacatt gtaattcttc tgtcattcga ttccacctcg ccagcccata
     6841 tcagggtgtt ttgctaatag ataggccata attctttttt cccaaaaacg attaaattgt
     6901 gtcttccaac tatctgttgc gataatgggt tttacaagga tgctacgtat ccctgcacga
     6961 ttggctccac gtatatcagt cataatctga tcgcctacca ttactatctc atcatctgct
     7021 aaatccaatt gtttctttgc tatgttgatc cctcttgcta aaggtttcat tgctcgtgca
     7081 acatacaaaa gatcaaattt ttcaatcgct cgcgcgacac gttttgaatt attgtttgaa
     7141 acaacaacaa cagggattcc tgcatttttc atttctaaaa tccaagtaag caactcttct
     7201 gttccatctg gattattcca tgcaatcaac gtattgtcta aatcggtcaa tactgctttg
     7261 atgcctagat tctttaattg ggcaggtgta atctgataga tggcttctac catccatgtt
     7321 ggtttgtaat ttgaaaacat gctgtctcct catctgcaaa aaatgaagga gcacataaag
     7381 tactcctcaa ccccgtatta tactgcatat cagcaaaaaa aactacctct atactcgttc
     7441 gtctttcaga aaaagcattt ctttacaaaa tgaccaaaaa taaagattga tcacagaaaa
     7501 atttagaaac aaaaaacaac taatattggc ggaacctctt gtatcgtttt tccagtaatt
     7561 tatgctgatc taatcgaaca agttccgtca atctttcaat caacgctttt ttcataatgt
     7621 gtgcgacttt atatcgtggc aactgctcgc cgttgaacgt ttcaggaata actcgatcaa
     7681 cgattcctaa ctcgcgtagt tctggcgcag taattttcat caactcagct gcttctgcgg
     7741 cgcgactacc gtctttccac aaaatcgacg caaatccttc tggagaaagc acagcataaa
     7801 tcgtttcttc cagcatccag acttcatccg ctaaacctag agcaagggca ccgccacttc
     7861 caccttcacc aatgatgatg gcgatgatcg gtacctttaa atcagacatt tcgagcagat
     7921 ttctggcaat agcctctcct tcgccgcgct cttctgctcc tacaccgcaa tatgcacctg
     7981 ctgtattgat caaggtaatc accggtctat tgaacttttc cgcttgtttc atcagtcgta
     8041 aagcttttcg atagccttca ggatggggag aaccaaaatt tctgcggata ttttcctgca
     8101 gatcccgtcc tttttgaata ccgatcactg ttaccggttt gccatctaat cgagcgattc
     8161 caccaacaat cgcttcgtca tctgcaaata aacgatcacc atggcattcc tcgaaagaat
     8221 cgaagatctc ttcaaaaaaa tccaagcttg tccatcgttc tttagctctt gctaactgaa
     8281 cgacatcatg tgctgtctta tccacgatct atccatcctt tctgtgtatg caattcaaga
     8341 agcgtatgga ttttctgttt caattccatg cggggaacaa tctgatcgac aaaaccatga
     8401 gataataaga attcagcctt ttggaaatct tctggaagtt cctgcttgat cgtctgttcg
     8461 atgacgcgtc ttccagcaaa accgatcaag ctttggggtt ctgctagaat aatatcgcct
     8521 tccattgcaa aactggcagt tactcctccg gtcgtcgggt ccgttaaaac agttagataa
     8581 aaaagacctt cattgctatg ccgtttgaca gcagcagaga tttttgccat ctgcattaga
     8641 gaaaagattc cttcttgcat ccgtgcgcca cctgaagctg tgaacaagac gactggaaga
     8701 ctttcctttg ttgctcgttc gaataatctg gtgatctttt ctccgacaat cgttcccata
     8761 cttcccatga taaaattagg atccatcact ccaataatga agggaatccc ctgaatggtt
     8821 gctttaccag tgaggactgc ttcatgaagt cctgttttat cttgcatttt tttaattttt
     8881 tccgtatatt cgggaaatgc tagcggatct ttggttacaa gatccgtatc ccattcttca
     8941 aaactttttt catcaataat caaagctaga cgctgccatg caccgattcg aaaagcgtat
     9001 ccgcaatgag ggcatacttt ttcttcaccg atgtctttcg tataaaggat atgtttgcaa
     9061 tttggacact ttgcccacat gttatcagga acagatggcg agttagcagg tttgctctct
     9121 ctattaggat tgatccgaat atatttcttt ttcttaaaca gagccatcta tgcgcctcct
     9181 atctccgttt ctggtgtcca attcggtaaa aattcttctt gtaaaaatgc tgtgctgtaa
     9241 tcacctgcta tgactgcagg atgactaatc aaatccatct gaaattctgc gttcgtgatg
     9301 attccttctg tgataaattc acttaaggct cgctgcatct tcattaatgc ttcaaaacgc
     9361 gtatttccat ggacgattat ttttgcaatc attgaatcat aatatggcgg gatcgtatat
     9421 cccgggtaaa ctgcactgtc tactcgcagt cccatcccac ctgctggaag cagtaagttt
     9481 tggatcttcc ctggagaagg agcaaaatgg aaagcaggat tttctgcatt aatccggcat
     9541 tcgattgcgt gtccttggaa tactacttct tcttgacaca cattcaaagg ctcaccggct
     9601 gctatctcca cctgtttttt tactaaatcg attcctgtga ccatttctgt tactggatgt
     9661 tccacttgga ttcgagtatt catttccatg aaataaaaat cgccttcttc atccatcaga
     9721 aactcgatcg ttccggcatt ttcataattg actgctttag ctgctcgaac agcagcgtct
     9781 cctagtgctt ctctttttgt ttgtgagatc actacagaag gagattcttc cagtactttt
     9841 tgattgttgc gttgtaaaga acaatctcgc tcgcctaaat gaatgacatg accaaatcga
     9901 tcacctaaga tctgaacttc tatatggcga gcaggataaa tgattttttc caaatacata
     9961 tcatcatttc caaatgccgc tgctgcttct tgctgagctg aagaaaaatg ctgtgggagc
    10021 tcttcttttg attgaacttt ccgaatgcct ttcccgccac cacctgcagc tgctttcagc
    10081 atcactggat aaccaattct gtctgctatt tccaatgctt cttctactgt agataataca
    10141 ccagtacttc ctggaatgac tgggacattt gcttcttgca tcaattgacg cgcattgatc
    10201 ttatttccca ttgcatcgat cgttgcactt ttaggaccaa taaatgtgat attgcactct
    10261 tcacacatgg aggcaaatcg gctattttca gataaaaaac caaatcctgg atgaattgct
    10321 tcggcttttg tcacgattgc agcgctcagt acttcctgaa cattcagata tgaatcaacg
    10381 gcttttgccg ggccaatgca gattgcttca tctgctaact gtgtatgaag tgcttcggca
    10441 tctgcttctg aatatatcgc tacggtttgg atccctagtt cgcggcatgc acgaatgata
    10501 cgtacagcga tttcaccgcg gttagctatc aatactttag aaaacatatt tcacctatcc
    10561 aatcataaag gttaattccg cttcggctac ttttttaccg tctactttag cgattccttt
    10621 gcctattccg gctgaagctt tcactttcaa tatctcaact tcaaggtata aggtatctcc
    10681 cggtgtaact ttcttacgga attttgcttt gtccaatccg ccgaaataag ccgtctttcc
    10741 cttgaattgt tcaagagaaa gtaatgcaac tgctcctgct tgagccattg cttcaacaat
    10801 caatacccct ggcataacag gttcttctgg aaaatgtcct tgaaaaaagg gctcattgat
    10861 cgttacattt ttcttagcaa cgatccgttc tccttcaacc atttcttcca ctcgatcaat
    10921 caaaagcatt ggataacgat gtggaatgat ttcttttatt tcttgtatgt tcattgtttt
    10981 ccctctttcc tgctttgctt atttttgctg aatcctaaag agtggctgtc catattcgac
    11041 aactgactcg ttttctacaa ggatttctgt gatcactccg tcggcctcac tagttatctc
    11101 attcatcagt ttcatcgctt caataatgca gataacttct cctttggcaa cttgatcacc
    11161 tacttttttg aaagctggtt tgtcgggcgc tggttgcaga taaacgattc ccacgattgg
    11221 cgaaacaacc tcgattccat caaatactgc cgattctttt tctttcatta tttctggagc
    11281 agttgttgct tgttcaggta tgacattgac tggctccgaa actggcaacg tttcgtttgt
    11341 ttgaaccata ttcatgcctc gacttgtttt atttttattc atatacaatt caaattgacc
    11401 ttcacgcaaa tcgaattccg ttagcgagga ttgatcaaat tgactgacta attctctgat
    11461 ttcattgata tccaaaatta ttcctcccag cgtttcatgc acagaaccgc attatgtccg
    11521 ccaaacccta atgaattact cattgcataa gtaaaacttg caggtttgct ttcgtttttg
    11581 acaactgtca agtcaatctc aggatcttgt ttttctacat tgatcgtagg tgggataaat
    11641 tgatgttcta atgccagcaa agttgccaag gcttcgatcc cgccggctgc tcccaataaa
    11701 tgtccagtca tcgatttcgt gctgctgacg taagtatctc tgaaattttc acctaatgcg
    11761 atttggattg cttttgcttc tgcctggtca ttggctggtg tacttgttcc gtgagcattg
    11821 atatatccga cttgttccgg ctgtacacca gcttcttcca tcgcaagctt cattgcttta
    11881 gatgcaccag aaccatctgg agtaggagct gtcatatggt aagcgtcaca gtttgcaccg
    11941 tatccaacga tttctccata gattttggca ccgcgagctt gtgcatgttc taaagattca
    12001 agaactagaa cgcctgctcc ttcacccatc acaaaaccat tccgattttc gtcaaacgga
    12061 atcgaaccac gattaggatc agttgattct gagagtgctg ttaaagctgc aaatccagca
    12121 atccccatct cacaaatcgt tgcttctgtt cctccagcaa gaatgacatc agaatagcca
    12181 tgtttgatgt tacggaatgc ttccccaatt gaattcgttc cgctagcaca agctgttacg
    12241 atagctgtac aaataccttt tgcacctaca cgcaaagcaa tatttccagc tgccatgttg
    12301 gcaatcgcca ttggtacgaa cattggagaa actcgttttg cacctttttc atgtagacga
    12361 ataacttggt tttcgattgt tggaagacca ccgatcccag atccgatgat gacgccaaac
    12421 cggtcttgat tcattttttc tttatctaaa ccgctcatct ccattgcttc taaagcagcg
    12481 tagattccat agatcgaaaa catatccatt cgtttgctat cttttttaat aaaatattta
    12541 tcgtaaggga aatctttgac ttcacccgct aaggtaatcc ctgtttctga agcatcaaat
    12601 ttagtgatcg gtgcgatgcc attcgtcccc tcttttaagc tattcaaaaa gctgtctgcg
    12661 tcatttccaa taggtgaagt gactccatag ccagtaatta ccactcgatt catttgtttg
    12721 ctctcctttt cttatccgtg catgaccaat ccgccatcga cattgatgac ttgtccggtg
    12781 atatatggat tttgtgctaa gaaaactgct gtttgtgcaa tatcttctac ttgtccgaaa
    12841 cgctgcatcg gaatttgctt ttccgcttgt tccttgactt tgtctgctaa aacttccgtc
    12901 atatcagttg tgatgaatcc tggcgcaatg gcattacatg taatgcctct agttgcagct
    12961 tctcgtgcca cagattttgt taatcctact acgccagctt tactagctgc ataatttgct
    13021 tgaccaatat ttccaatcaa cccagataca ctagagagat taatgatggc cccttctcgc
    13081 tgcttcatca tttttttcaa tacgtgctgc gtcatattga aggttccaat caaattgata
    13141 tccagacatt ttttgaaatc ttctgcatcc attcgcatca caagtttgtc atttgtgatt
    13201 ccggcattgt ttactaacac atggatcgat ccaagctttt cttctgcttc tttgatcatt
    13261 tgtcccgctt tctcataatc agagatgtca ccggaaactc ctacgcattt tacaccgaat
    13321 gcttcgattt cttctatctt ttcttttgga atctctccgc gaccattcaa aatgatattt
    13381 gctccagctt ttgcaaaagc taaggccatc gctttgccga ttcctcttgt actacctgtg
    13441 ataaatacat tttttccttt tacatccata acgttcctcc ttatagtatt gctattgttt
    13501 catcaaatgt ctgcttattc tcaacacgca gaactggtat tgttttatcg atttttttca
    13561 taaatccgct taatactttt ccggggccga cttcgatgac ttgttcgatg ccgattgttt
    13621 ttaacttatg gatactctca tagaaacgaa caggttgcat gacttgctgt tcaagtagtc
    13681 ccgcgattgt ttctttcttc attatttccg ttgttgtgtt gcttatgata ggaaaagaag
    13741 gttcagagaa ttgaatctga cttaggtcct tcgccagttt ttttgctgcc ggttctaaaa
    13801 tagctgtatg aaaaggcccg ctgacattta gagggatcat tcttttgaac cctttttctt
    13861 ttaatagtaa aacagcttcg tctactgctt tttcttcgcc accaatcacg atctgctgag
    13921 gtgtattata atttgcagga gatacgatgc cgtacttgct agcttcatga cagctttcct
    13981 cgatcgtttc aatcggcgca ttcatcactg caaccatttt cccgcttcca gctggggcag
    14041 cttctgtcat ataagcgcct ctttttgcta ctaacgcaac tgcttcagtg aaggatagcg
    14101 cattactagc tactaatgcc gaatattctc ctaaactcag tcctaaagct gcatctggga
    14161 tgattccatg ctccttcaat aagcgataat acgcgatgct gactgttaga atagctggct
    14221 gtgtatattg cgtctgatcc aaacgttcat tttctgtaaa gcaaagttca gccatatcgt
    14281 agccgagaat ctcacttgct tcatcaaatg tctgctttac aacggcttct tcatataaat
    14341 cttttcccat accttgatat tgtgcacctt ggccactaaa taagaatgct gttttcatgt
    14401 ttttctccaa tatatccaga atatatgttt gattcttcct attcaaaaag gccgagacat
    14461 tcctatttca ggaaatgtcc cagcccccaa cctaatttta atgattatgc agtttgtttt
    14521 tctacgtaag tcactaaatc ttgaaccgtt ttgattccgt cttctgtttc gattttcacg
    14581 tcaaattcat cttcaatttc attgatgatt tggaataaat ccaagctgtc agcttctaag
    14641 tcttcttgga tgtcagtagt caatttgatt tcttcagttt cttttcctaa ttcttctgcg
    14701 atgatttctt gaattttttc gaataccata ataatatatt cctccgatat tgtttgcttt
    14761 ttatttttat aatttaacag tctacaactc catgagaata cttccccatg ttaaacctcc
    14821 gccatagcct gtaaatatta ctttctgctg ttttcctaag gtgatctttc cactagaaac
    14881 tgcttcatcc aataagatag ggatgcttgc agcagatgta ttcccatatt tatccatatt
    14941 ggtcaggaac ttttcttgcg gcatctttac ttttcgggca attttctcaa taattctgag
    15001 gtttgcctga tgcaaaagca gataatcaac tggtttgtct ttcatgactt cttggatatt
    15061 gtttgcaacg tctcgtacgg caaaatcaaa aatatctctg ccagtcattt gcaaataata
    15121 cgaaccttct gaatcttctg aatagaacgg atttttattt tgctggtagc cagcggtcaa
    15181 tgagctactt ctggtaccgt cagctgctaa tttttctttc agcaattttt tctgtggatt
    15241 cgcttctaag agaatcccac ctgctccatc gccaaacaac acagcagtcg tacgatcaga
    15301 ccaatcgatt agcttagata gtgtttcacc accaatcacc agtcccttag ttgagccgca
    15361 gcggatcatt ttttcagcca tgcttaacgc ataaacaaat cctgagcacg cagcactgat
    15421 atcaaaagcg atagcatgag ttgctcccaa tttcccttgt acttgtgctg ctacagatgg
    15481 tgtcgtgtaa tcgggcgaca ttgttgctac taaaataaaa tccaaatctt tagggtttag
    15541 atttttctgt tttatcaatt tttccgccac taaaatgcat aaatcagaag tctcctgatt
    15601 tgttacacat cttcgttcac gaatacccgt ccttgacgaa atccattcat cagaggtatc
    15661 catcatcttg gaaagatcat tattagtaat aatcttttca ggaaggacgc tggctgttgc
    15721 agtaatcatt ccaaattgtt ccattaaata tcctcactta tagtcttgca agaagtcgtg
    15781 caagtttttc agtgcatcca gcaaggcatg ctgttcctcc gtagccattc cgtctaagat
    15841 gttcttgacc atttcacgat gaaaatgctg atggacccga aatagtaact ttcctttttt
    15901 cgttagtccc aactttacaa cgcggcggtc atcttcactg cggatacgtt cgacatagcc
    15961 tttttttact aacttgttaa tagcagtggt caaggtccca acagtaattg atagttcttt
    16021 ggctacttct gaagttgttt ttttcttgta catcccgatt gcctcgatcg tgtgcatctc
    16081 tgtgattgac agatctttga attgagattt tttcagctca gactcttcga ttgtcaaaat
    16141 atcattgaag acactaacaa gataatcatt tactgttttt aaatctggtt ccatgttcca
    16201 ccgtccacta attagtttga ttatcaaatc ttttgatgtt caaactatat cttaactttt
    16261 ttcaaaatgc aaccttaaaa gatcatttt
//
LOCUS       DANKHL010000052        15820 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000052 DANKHL010000000
VERSION     DANKHL010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.054"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            48..800
                     /locus_tag="PW101_001174"
     CDS             48..800
                     /locus_tag="PW101_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2513586.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            797..1711
                     /gene="pfkB"
                     /locus_tag="PW101_001175"
     CDS             797..1711
                     /gene="pfkB"
                     /locus_tag="PW101_001175"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL2513587.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1729..3660
                     /locus_tag="PW101_001176"
     CDS             1729..3660
                     /locus_tag="PW101_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2513588.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3771..5330)
                     /locus_tag="PW101_001177"
     CDS             complement(3771..5330)
                     /locus_tag="PW101_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HDL2513589.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5485..7719
                     /gene="pcrA"
                     /locus_tag="PW101_001178"
     CDS             5485..7719
                     /gene="pcrA"
                     /locus_tag="PW101_001178"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDL2513590.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7736..9772
                     /gene="ligA"
                     /locus_tag="PW101_001179"
     CDS             7736..9772
                     /gene="ligA"
                     /locus_tag="PW101_001179"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDL2513591.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9878..10183
                     /gene="gatC"
                     /locus_tag="PW101_001180"
     CDS             9878..10183
                     /gene="gatC"
                     /locus_tag="PW101_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDL2513592.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10183..11655
                     /gene="gatA"
                     /locus_tag="PW101_001181"
     CDS             10183..11655
                     /gene="gatA"
                     /locus_tag="PW101_001181"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDL2513593.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11652..13082
                     /gene="gatB"
                     /locus_tag="PW101_001182"
     CDS             11652..13082
                     /gene="gatB"
                     /locus_tag="PW101_001182"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDL2513594.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13092..14147
                     /locus_tag="PW101_001183"
     CDS             13092..14147
                     /locus_tag="PW101_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL2513595.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14258..15631
                     /gene="rlmD"
                     /locus_tag="PW101_001184"
     CDS             14258..15631
                     /gene="rlmD"
                     /locus_tag="PW101_001184"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL2513596.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15634..15762
                     /locus_tag="PW101_001185"
     CDS             15634..15762
                     /locus_tag="PW101_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL2513597.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         5384 a         2898 c         3473 g         4065 t
ORIGIN      
        1 gagtagaaaa aaaatatgct attatttgta ttgaggtgat cgtgaaaatg cttacagagg
       61 aacgtagaca aaaaatccta gacttgcttg aacagcagaa aatcgtgaaa tcacaagatc
      121 ttgtaaacct ttttaatgct tctgaatcaa cgataagacg tgatttacaa gagctggaga
      181 atagtgggtt tctagaaaga gttcatggcg gagctaagaa agagcaatta ttgaattttg
      241 agcagaatat gagtgaaaaa tcactcaaaa acgttcacga aaaacagcaa atcgccaaat
      301 cggcagctga aaccattaat gacgaagatg tcatttattt agacgctggc tctactacgt
      361 tagaaatgat tccttttctg caaggaaaac agatcacagt agtgacaaat tccgttcaac
      421 atgcggcaaa tttagttgat ttggagcttg ctaccattat tttaggtgga acgataaagc
      481 tggcaactaa tgctgtttta ggatctaatg cgatagaaca actaaaaaat tttcatttta
      541 ataaagcatt tatgggaatg aacggtgccc atcttgaaca aggattcacg actccggacc
      601 cagaagaggc agctgttaaa cgtttagcca tgaaaaattc cgaagagaca tttgtattga
      661 ttgatcatac caaattcaac cagttgtcct tcacctctgt tgcacctttg gaagcagcca
      721 cgatcataac cgagcgttgt ccattagaat tcgttaatga attccgtgaa aagacaacaa
      781 ttaaggaggc aatacaatga tttacacagt aacacttaat ccatcaattg attatatcgt
      841 acgcgtagat gggttaaaac taggcgattt gaaccgaatg aaggaagact tcaagttacc
      901 tggaggaaaa gggatcaatg tttcccgtat tttgaaacgg atcgattctc aatctacagc
      961 actagggttt ttaggagggt tcactggcga atttatttcg aagtggcttg aaaaagaaca
     1021 aatccagtct ttgttcacaa ccgtaaaaga agatacaaga atcaatatca aactaaaatc
     1081 agatactgaa actgaaatta acggtttggg cccatctatt tcagatgaag aaatcgatga
     1141 gttgaagcaa gcattaggaa cagtaggaca aggagatatc gtcgtattgt caggaagcgc
     1201 gccggctagc ctgcgaaaag gattttatca agaactgatt gatatcattc gagaaaaagg
     1261 tgcagatttc gtgattgata caacaggtga tgacctaaaa aatgcacttg ttaagaaacc
     1321 attgttgatc aaaccaaaca atcacgaatt agctgagctt tacaatacaa ccttccaatc
     1381 tgtagaagaa atcattccgt atggtaaaaa attgctagct gaaggggcac agaatgtgct
     1441 gatctcaatg gctggagacg gtgctttgct cttcacggaa caaggaactt ataaaacgaa
     1501 tgtattagtt cgcccattga aaaattcagt aggtgcaggg gattcaatga ttgccggctt
     1561 tattggggct tacagccgta cgaaagatcc aatcgaagcc tttaaatggg gagttgcttg
     1621 cggaagcgca acagcttttt ctgatgactt ggcagctcgc gaattgattg atgagttgct
     1681 gccagaagtt gaaatcacaa aaattgatta gtttcaagga ggataatcat gaatatcaag
     1741 gatttaatgg tcaaagaagc gatgatcatg gatttgcaag cgactgataa aaaaggcgca
     1801 atcgatgaaa tggtccaaaa aatgtttgat gccggccgga tttccgatat cgaaacatat
     1861 aaagaaggaa ttctagcccg cgaagcacaa acttctacag gtcttggtga tggaatcgcc
     1921 atgcctcacg cgaaaaacag tgcagttaaa gaagcaacag ttttattcgc aaaaagccaa
     1981 aaaggagtag attatgaagc cttagacgga cagccaactt atctgttttt catgatcgca
     2041 gctccagaag gcgcaaacga cacacatttg caagcattag cagcactatc tcgattgctg
     2101 attgatgcag atttcgtagg aaaattaaaa gaagcaaata caccagaaga agtccaagac
     2161 ttgttccaaa cagctgaaga gcaaaaagaa gcagaagaaa aagcagaaca agaaacacaa
     2221 gctgcagaag cttctgctca tccagaaaga aaattcgttg ttgccgttac agcttgtcca
     2281 acagggatcg ctcacactta catggcagaa gatgctctga agaaaaaagc gaaagaaatg
     2341 ggtgtggaga tcaaagtcga aacgaacggt tcagaaggaa tcaaaaaccg attgactgct
     2401 gaagacatcg cccgtgcaga aggtgtaatc gttgcagcag acaaaaaagt agagatgaat
     2461 cgctttgacg gaaaagaact gatcaatcgt cctgtcagtg atggtatccg taaaccagaa
     2521 gagctgatca atctagccct tagtggtact gcaccagtat tccatggtga tggaaaagaa
     2581 agcagttcag aagaaggaaa tgcggatggt actatcggac aaagaattta caaagatttg
     2641 atgaacggcg tatcacacat gttacctttc gttattggtg gaggaatcgc tattgcgctt
     2701 tctttcatga tcgaccaatt tattggcgtg ccacatgatc aattatcaaa tctagggaat
     2761 tacaatcaag ctgctagctg gttcaaccaa atcggacaag ctgcatttgg tttcatgctg
     2821 ccggtattag caggatttat tgcttcaagt atcggtgacc gccctggttt gatcgttgga
     2881 tttgctgctg gtgcattagc taatacaggc ggtgcaggat tcttaggtgc actgattggt
     2941 ggattcttag ctggttatgt catcgtattt ttacgtaaat tattcaagaa cttaccaaaa
     3001 tcattggatg gaattaaaac gatcctcttt tatccagtgt ttggcttact gattactggt
     3061 ttcttgatgt tgctggtcaa tgtccctatg aaagcaatca atgatgcatt gaacggtttc
     3121 ttgacaagta tgagtggttc gaatgctgct ttacttggtg cattattagc tggaatgatg
     3181 gcggcagatt taggcggtcc aatcaacaaa gccgcttatg tgtttggtac agcaacttta
     3241 gctacgactg ttgcaactgg cggaagtgtc gtgatggctt ctgttatggc tggaggaatg
     3301 gtacctccat tggcaatttt cgttgctaca cgtttgttta agaataaatt cagcaaaaca
     3361 gatcaagatg ctggtttgac gaacatcgtg atgggactat cgtttgtcac ggaaggatcg
     3421 attccatttg cagcagctga cccgattcgt gccattccaa gttttattat tggttctgct
     3481 ttaactggcg gattagtcgg agctttcggg atcaaattac tggctcctca tggcgggatt
     3541 ttcgttgtat tcttactgag tcacccggtc atgtatttgg tgtttattgc tatcggagca
     3601 gtcgtatctg gtgtgattta tggtttattg agaaaagctc ctgcagcagc agaagcatag
     3661 aaataaaaaa gaagaggctg gtttttccca gtctcttctt tttttataga atcatataga
     3721 atcaagtagt gtagatgaat agttgttgta aaatagtttt tccatgttcg ttaaaatgat
     3781 tgcttcaaat ttgtgataga agatttagcc tcgatttcct gcttgattct tggcaagttt
     3841 gcttcatcag tcaagataac caaaatatct cctacattca tgatcgtatc accgtgagtc
     3901 aagatttcct gatttcctct gcgtatagag attagcagca tatttttagg ccaattgaag
     3961 tctcgtacca ttaatccgtc taaagaactc tctgccgtta ctggaaaatc aaaagttgtc
     4021 cttgcacctt ttagtgaggc ggtctgagta tttccaagta gacgttccaa taatgattcg
     4081 taaattggat taccgccgag aaggtcattg acgatataag caacaagagc agccaatcct
     4141 aaaggcatca gatgattaag agagccaacc atttctgtca ctaaaataat tgctgtcagc
     4201 ggtgcttttc caatcgctgt gaaataacca gccatggcaa taacgacaaa atccttgatg
     4261 tagattggat ccatcccgaa atcaaccata atgtttccat aaaagctacc gattaaagcc
     4321 cccaatgtca atatcggaag gaaaatcccc cctggaagac caccaccgta agaaatcatg
     4381 gaaaaaacga atcgaagaac aaacaaaccg ataatcataa tgattggcaa ggaagcatgt
     4441 ccaaaagcta aaacgatttg attgcctccg cctagagtac taggccataa ataaccaatt
     4501 ggcaaaatca gcaagaaagg aacgaacccg tgaaaataag ctggtaaagg caatcggttg
     4561 taaaattttg gcaaagccag caagaccttc tgataaataa aacctaagat gcctagaagt
     4621 actcctaaga tcactaagga accataatat ttcaacggca aagaaggaag gtcacctaga
     4681 tataaaacag gtctcaagcc gaaaaagtaa agagagacga agttagcagt gattgctgaa
     4741 gcaaatgatg tcagccaaac aagtggagaa aaggagtgat ggatttcttc taaaacaaag
     4801 agtaatcctg cgataggagc attgaatgct gctgcaagtc cgctacttgc accgctagaa
     4861 attaaaatct tttcttctga agatggactc cgaaacaatt gtccagctcc ttgtccaatg
     4921 gatgcgccaa gctgtataga aggtccttct ctacctagaa aaaggcccgc accaactgaa
     4981 agtacaccgc caatgaactt tttccataaa acagagaacc agttcattga taattccccg
     5041 cgaagctgtc cctctacttg tggaatcccg cttcccttga tatccggttc atttttgacc
     5101 agataaccca cgaaaaatgc tgcaaggatg cagcaaatag ctaggccaat taagtaaatc
     5161 ggctgttccc gactcatttc atagatactt cgaactaaat cgcttagctt ttcgatagat
     5221 aatcgaaaca ggctaacgac gattcctgtc agcagtccaa caaatattcc ttttagaata
     5281 aaatatatct gagtcttatc taatttcttg atttttcctt ttttttccat tcaaacgcct
     5341 cgctttcttt taatttagcg cgaaatcaac gaattgacaa ggaaaaatcc ataaaaagaa
     5401 acaaatgatt ttgaactagc tagtttaatg aaagccaaga aaatgctata attaacaaga
     5461 ttgataaaag aaaggatgtt gttaatgtca tccaaagctg aattattaaa cgggatgaac
     5521 ccccgtcaga aagaagctgt tcttcacact gacggaccat tactgctaat ggcaggtgca
     5581 ggaagtggaa agacgagagt gctgacacac cgcattgcct atttgattga ggaaaaagaa
     5641 gtcaatcctt ggaacatcct agcgattact tttaccaata aggctgcaaa agaaatgaaa
     5701 gaacgtgtca atgcgattct cgcatctgga ggcgaagatg tctgggtatc gacttttcac
     5761 tcgatgtgtg tgcgtatttt aagaagagat gttgatttta tcgggtataa ccggaacttt
     5821 acaatcatcg attcttctga acagctgacc ttgatgaaac ggatactaaa agaactgaac
     5881 attgatccga aaaaatatga tcctcgaagt atattaggaa caatctctca agcaaaaaac
     5941 agcctgcaga cccctcagga ttttgcaaaa atgcaaggca gttattacga agagattgcg
     6001 gcaaaatgtt atgctgctta ccaaaaagag cttcaatata atcaatgtat ggactttgac
     6061 gacctgatca tgaatacgat tcgattattt gaagaacatc ctgattcact cacatattat
     6121 caaaacaaat tccattatat tcatgtagat gagtaccaag atacaaatca cgcacagtat
     6181 acgctagtca atttattggc aggtagattt aggaacctgt gcgtcgtggg agatgcagat
     6241 caaagtattt acggctggcg cggtgcagat atgcagaaca tcttggattt tgaaaaagat
     6301 tatccagatg ctgcagtaat tttgctagaa caaaactatc gctccacgaa aaacatcctt
     6361 agcgcagcaa accaagtcat tgaaaacaat agcaatcgaa aaccaaaaaa cttgtggaca
     6421 gaaaacaaag aaggaaacaa gatcacttac taccgtgcag ataatgaacg agacgaaacc
     6481 cgttttatcg tagatcggat gcaagaagag attcgaagca atcatcggaa ttacggcgac
     6541 tttgccatct tgtaccggac gaacgcacaa tctcgtgtaa tggaagaaac tctgttgaaa
     6601 gcaaatattc cttataaaat ggttggaggg cacaagttct acgatcgtaa agaaatcaaa
     6661 gatattttag cgtatttgaa tgtgctggca aatcctcaag attcaatcag ttttgagcgt
     6721 atcgttaatt cgcccaaacg aggcatcgga ccaggatcta tcgaaaaact acgcagtttt
     6781 gcttctcttc atgaatggcc actgcttgaa gcagcgcaaa atgtggatct ggcaaatatc
     6841 ggtggaaaag caggacagca attaggtgca tttggagaga tgatccaaga agtcactcag
     6901 atgatcccat acttgacagt gacagaattg acgaaagagg ttttggatcg tagcggatat
     6961 ctagaagact taaaaatcca aaatacatta gaagcacaag cacgcatcga aaacttggag
     7021 gaatttttaa cggtcacaca agaattcgac aaacaattcg aacagcaaaa tgaagaagac
     7081 gctgatgcac cagaagaaaa actaacggtc ttcttaaatg atctggcact tgtttccgat
     7141 attgacaatt tagaagaaga tgcttcccaa gtgacattga tgactttgca tgcagccaaa
     7201 ggattagaat tcccagttgt attcctgatt ggtctagaag agggggtttt tcctttatct
     7261 cgagcattga tggaagaaag cgaactggaa gaagaacgcc gtctcgcata tgtggggatc
     7321 acgcgagcag aagaagcgtt gtatttaaca aatgctttct ccagaacatt atatggacgg
     7381 acgcaatata accgaccttc tcgttttgta gaagaaatcg atcaggagtt actggaaatc
     7441 gaaggcatgc gtcctacgcc taaaaaaaca cctgtattcg ctaaaaaaac agcatatagc
     7501 tataaacagc cagagacagc ggttgttccc tctaaaagtg ctactggagg ggaaaataac
     7561 agctggaaac ctggagataa agtcaaacac aaaaaatggg gtctaggaac agtcgtccga
     7621 gtaagcggga cgtcgaagga tctagaacta gatgtggctt tcccaagtca aggagtaaaa
     7681 cgtcttttgg ctgcctttgc gccaattgaa aaagcttagt agtggagggt aaatgatgga
     7741 acatgaaatg acgattgacg aagctgctaa acgagcagaa gaacttcgta caagactgaa
     7801 ccaatggtct cgcgaatact acgtagaaga taaaccaaca gtggaagact atgtttatga
     7861 taaagaatac gcagaattag tagcaattga ggaacaatat cctgatttga tcacgtctga
     7921 ttctcccact caacgagtag gcggaaaagt attagaaggt tttgaaaaag tcactcatga
     7981 tattccgctt tacagtttga acgacgtttt cagcaaagaa gagctgatag cttttgatca
     8041 acgtgtacaa aaagcagtgg gacgtgtagt tgactattgc tgtgaactaa aaatagatgg
     8101 actttccgtt tctttgcgtt atgaagatgg aaatttcgtg agaggtgcga ctcgcgggga
     8161 tggaactgta ggagaaaaca tcactgaaaa cctaaaaact gtccggtcgg tcccaatcaa
     8221 attaaaagaa ccaatgaaca tcgaagtaag aggcgaatgt ttcatgccaa aacggtcatt
     8281 tgtccaattg aaccaagaca gagaagcaga gggaaaagat atctttgcga atcctcggaa
     8341 cgccgcagct ggttctttgc gtcagttaga ttctaaaatc acagcgaaaa gaaatctgga
     8401 cactttttta tacacagtag ctgatttcgg tcctatgcaa gcaaaaaccc agtatgatgc
     8461 gctagaagag ctagaaaaaa ttggattcca cacgaatcgg gaaaaacgtc tatgtcattc
     8521 gattgatgaa gtgtggtcat acattgaaga atatcatgac aaacgtgtgg atttgcccta
     8581 tgaaatcgat ggaatcgtca tcaaagtgaa tgaattctcg ctacaagatc aacttggttt
     8641 tactgtcaaa gcaccgagat gggcagcagc ttacaagttt ccgccagaag aagttgaaac
     8701 gctaatcgaa aacatcgaat ggacagtcgg tcgaacaggg gtagtaacgc caacagctat
     8761 catgacacca gtccgagtag caggaacgac agtaagccgt gccagcctgc ataacggtga
     8821 ttatattaag ttgaaggata tccgcttgaa agatacagtt ctgatttata aagcgggaga
     8881 catcatccca gaagtttcgc aagtcgtgtt agataaacga cctaaagaca gcgaagaata
     8941 ccaattgcct acacattgtc cagtttgtgg aagtgaactt gtccatttgg atgaagaagt
     9001 agccttacgg tgcatcaatc ctaaatgccc tgctcaaatg aaagaaggtt tgaaccactt
     9061 tgtttcaaga aatgcgatga atatcgatgg gttggggccg cgcgtactag aacagatgta
     9121 tgataaaaaa ctagtagcag atgttgctga tctttacaaa ttgacagaag aagagttatt
     9181 gacgttggac aaaataaaag aaaaatcagc aaataatatt ttgacagcaa tcgacaacag
     9241 caaagataat tcggtcgagc ggttgatttt cgggttagga atccgacatg tcggagcgaa
     9301 agcagcaaaa attttagcag aacatttcgg tgatctagaa acattgagta aaagtgacta
     9361 tgagtcgatt attgcgcttg atacgatcgg tgatatcatt gccgacagtg tagtcactta
     9421 tttcagtaac gaagaagtcc atgaattgat gaatgaatta aaacaagcag gcgtcaattt
     9481 cgaatacaaa gggcttcgaa atgcccaatt gcaagaagtc gagtctcctt ttaaagaaaa
     9541 aacagtagtc cttactggta aactaacacg atttactcgt gaagaagcaa aagagacgat
     9601 cgaaaatctt ggcgggaaag tgactggtag cgtatcgaaa aaaacggata tcgtggtagc
     9661 aggagaagat gctggcagca agctgaccaa agcacaagaa ttaggaatcg aagtttggac
     9721 agaagatcaa atggcagatg cattggctaa aagtcgctca gtagaagaat gaaaaatgag
     9781 ttttttcaga caaatttcaa taaacaattt cagacacggc actcttgtgt taaaatagag
     9841 agcaaagttt ttaactcgtt tagaaagaag gggtagtatg gcgattagtg aagaacaagt
     9901 gaaacacgtt gccaaattag caaaacttgc gttcgcagat gaagaacttg caagttttac
     9961 cgaccaatta ggtaaaatca tcgatatggt tgaaatgctg gaagaagtag atacagaagg
    10021 tgttccattt acgtcgaatg tggttgaaac agtaaacgtg atgcgacaag atcgggcgca
    10081 agctggatgg aaccgcgatg aattattgaa aaatgtgccg gaaacagaag acggctttat
    10141 caaagtaccc gcaatcatcg ataatgggga ggctggcgca taatgacaga attacacaat
    10201 cgttctctag aagaactgca tcatatgctt gtatcgaaag aaatcactgt acaagatttg
    10261 acaaaagcaa catttgaacg aatcaaagaa acagagcctg aggtcgaagc attcattaca
    10321 ttgaacgaag aaaaagcatt ggaacaggca aaagctttag atgaaaaagg gattgatgaa
    10381 agcaacgtcc ttgctggtat tccaattggt atcaaggata atatcgtaac aaaagatatc
    10441 ttgacaaccg ctgcatctaa gatcctttac aattttgaac caatttatga tgcaacggtc
    10501 atggataaaa tctatcaagc agatatgatc cctgttggta agctaaacat ggatgaattt
    10561 gctatgggtg gaagtacaga aacttcttat ttcaagaaaa caaaaaatgc ttgggaccca
    10621 acaaaagtac ctggtggctc ttctggtggt tctgcagcag cagttgcagc tggacaaatt
    10681 ccagtttctt taggtagtga tactggggga agtatccgcc aaccagcagc ttttaacggc
    10741 atcgtaggga tgaaacctac ttatggccgt gtctcacgtt ttggtttgat tgcctttgct
    10801 tcatctcttg accaaatcgg accaatgacc cgtacggtga aagataatgc attagcattg
    10861 acagctatca gcggttatga cgaaaaagac ggtacttctt caggcgtttc agttcctgat
    10921 tttgcagagg gactgacagg cgacatcaaa ggaatgaaga ttgctttacc taaagaatac
    10981 ttaggcgaag gtgtggcccc tggcgtaaaa gaagctgtat tgaaagcagc agaaacattc
    11041 cgttcattag gtgctgttgt ggaagaagtc agcttgcctc attcaaaata cggtgtggct
    11101 gtttattata tcattgcctc atcagaagca agttccaact tgcaacgatt cgatggtatc
    11161 cgctacggtt atcgctcaga aaatgtgaaa tcattagatg atgtatatgt caactcacgt
    11221 tcagaaggat tcggtgaaga agtaaaacgc cgtatcatgt taggtacatt ctccttaagt
    11281 gctggttatt atgacgctta cttcaaaaaa gcgggacaag tccgtacatt gatcaaacga
    11341 gattttgaaa aagtctttgc agattatgac ttgatcatcg ggccatcttc accaacagtt
    11401 gcattcggaa tcggtgagaa cattaacgat ccaatcacaa tgtacatgag tgacatctta
    11461 acgatccctg tcaacttggc tggacttcca ggtatgtctg taccggcagg tttatcagaa
    11521 ggattaccag ttggattgca gatcatcggt aattattttg atgaaaaaac gatgtatcaa
    11581 gcggcatatg cgtttgaaca agcaacagaa ttccataaac aacaaccagc tattttagga
    11641 gggaaagacg aatgaatttt gaaacagtca ttggactaga agtccacgtc gagttaaaaa
    11701 ccaactcaaa aattttctct ccagcgccag ctcattttgg tgcagaacca aacagcaata
    11761 caaacgtgat cgactggggt tatccaggcg tgctgccagt aatgaataaa gctgcgctgg
    11821 aattcgggat gaaagcagct ttggcattaa actgtgaaat ctctaaagat actcattttg
    11881 accggaaaaa ttacttttac ccagataatc caaaagcata tcaaatttcc caatttgatc
    11941 aaccaatcgg tcatgacgga tggatcgaaa tcgaagtaga aggtaaaaag aaaaaaatcc
    12001 ggatcgaacg tgtccattta gaagaagatg ctggaaaaaa catgcatggt attggcggtt
    12061 attcttacgt tgatttgaac cgacaaggca cgcctttgat cgaaatcgtt tctgaagctg
    12121 atatgcgttc accagaagaa gcatacgctt atctagaagc attgcgatca attatccaat
    12181 ttacagaagt cagcgatgtg aaaatggaag aaggctctat gcgctgtgat gccaatattt
    12241 ctcttcgccc ttatggacaa gaagagttcg gtacgaaagc agaattgaaa aacttgaact
    12301 ctttatcttt cgtgaaaaaa ggtttagctt ttgaagaaaa acgtcaagca aaagttcttc
    12361 tttcaggagg agaaatccaa caagaaacac gccgttttga cgaaacaacg aataaaacag
    12421 ttttgatgcg tgtcaaagaa gggtctagtg attatcgtta cttcccagag cctgatatcc
    12481 cgaaattcgt gatcgatgac gaatggattc aaaaagtaaa agctagtttg cctgagatgc
    12541 cagcttctcg tcgcgaacgc tatatccgtg agtttggact tccagagtat gacgcaatgg
    12601 tattgacact tacaaaagaa atgtctgatt tttttgaagc tgttttggaa aatggtgcag
    12661 atgccaagca agcatctaac tggttgatgg gtgaagtttc tgcttacttg aacagtgaga
    12721 aactggaatt agctgagaca aaattgacac ctgagaactt agcaggaatg attcgtttga
    12781 tcgccgatgg aacgatcagc tcaaaaatcg ccaaaaaagt cttccgtgaa ttgatcacaa
    12841 atggcggaga agcccaagaa gtagttgaaa aaaatggttg ggtgcaatta tctgatccga
    12901 acaaactact gccaatcatt aatgaaattt tggataacaa ccaacaatct gtcgatgatt
    12961 tcaaaaatgg aaaagatcgt gcggttggct tcctagtcgg tcaaatcatg aaagcaacta
    13021 aaggacaagc aaatcctggt gtcgtgaata agctacttaa agatgaatta gcgaaacgat
    13081 agaggctgaa aatgaaaaaa gcacgcgtaa tttataaccc cacctcggga aaagaattat
    13141 tgaaaaaaaa tttggcggat atcctacagg cattagaaga agcaggattt gaagccagcg
    13201 cctatgcaac tacaccagaa ccagattcag ccaagaatga agcatgtaga gtcgcagaac
    13261 taggttttga tctagtagtg gcagctggtg gcgatggcac aatcaacgaa gtggttaatg
    13321 gaatcgctcc gcttgaacac cgtcctaaaa tggccatcat cccagctggt acaacgaacg
    13381 actatgctcg agcattaaaa attcctagag ataatattaa agcagcagca gaagtgataa
    13441 aaaaacacca aaccgtaaaa atggacatcg gcctttctga taacagttac ttcatcaata
    13501 tcgctgcagg tggctatttg acagaattga cttatgaagt tccatcagaa ctaaagagta
    13561 tttttggtta tcttgcttat cttgcgaaag gagcagagat gctccctcga gtaaagccaa
    13621 tcaaaatgcg cttgaagtat gatgaagggg aatatatcgg aaatgcttcg atgtttttcc
    13681 tagggctgac gaactctgtt ggaggattcg aacagatcgc gccagatgcc aaattggatg
    13741 atgggaagtt ttctttaatc attgtgaaga cagcaaatat ctttgagatc ttgcatattg
    13801 cagcactcat gctgaatggc ggtcgacatg tcgatgatcc aagaataatc tataccaaaa
    13861 caagcagttt gttcgttgaa acagaagatg atatcaatcg accagtaatg atcaacttag
    13921 atggtgaata cggcggggaa gcaccgatgc atttccaaaa tcttcatcaa cacattgagt
    13981 tctttgccaa taccgatcag attcctgatg aagcaatcac aggtacagat gaagaagaat
    14041 tagaggttgt cagcaaagaa ttcgtaaaag aagtcgaacg tttaacggat gaagatatcg
    14101 acggtgacgg aaaaatagca gacgaaacac ctaatcaaaa gaattaatca aaagccggtg
    14161 ctgaacgtag taaggtcagt gccggtttta gttgtgtaat tagctgattt atagtagagt
    14221 tcagtaggat tgaaaaatag gcttaaagga gcaaaatatg acagaacagc aaatagtaaa
    14281 gaaaaacgaa cgatatacag tcgaaatcgt cgacttgagt tatgaaggaa tgggggtagc
    14341 aaaaatcgat ggttatccat tatttatcga aaatgcactc cctggagaag aagtagaaat
    14401 actagtagta aaagcaggca ataagtttgg atatggaaaa gtcgaaacat ttattacaca
    14461 atcacctgat cgtcagcctg tagaaaacaa tgcattactt cgcataggta tcgcaccttt
    14521 agcccatttg aagtatgaac aacagcttgt cttcaaacaa aaacaagtag agcatgtaat
    14581 ggcgaagatt gctaagatgc cagaagttcc tgtacttcca acggtcggaa tggcagaacc
    14641 attcggctac cgtaataaag cacagatccc tgttcgccgt atcgatggac agttagccac
    14701 cggtttttac aaaaagaata gccatgatct agtccctatc gaagattttt atatccaaga
    14761 tcctgtcatc gatcaagcaa tcaaagtagt cagagatatc ttgcaacggt tccaagtacg
    14821 aggatataat gaagaaaaaa atgaaggaca gcttcgtcac atcattatcc gtcgtggtca
    14881 ttattcccat gaaatgatgg tcgttttagt tactcgtaaa gaaaaattct ttaaatccaa
    14941 ggaaattgct gaagctattc atgaagaatt gccagaagtg gtttcggtca tccaaaatat
    15001 caatgaagaa aaaacaaatg tgattttagg agacaaagaa aaagttttat atggcagaga
    15061 ctatatcgaa gatcagctat taggaaaaac ctatcgaatc tcagcaaagt cattttatca
    15121 agtaaatact caacaagcag agtatctgta ccaaacagcc atcgagtttg ccgagttaga
    15181 taaaaaagac attgtgatcg acgcttattc aggaataggt acgattggct tgtctttagc
    15241 agacagagtg gataaggtgt atgggatgga agttattcca gaagcaattg aagatgcaca
    15301 gttcaatgct ttaaataata aaatcgaaaa tgttcattat gaagtcggaa aagcagaaaa
    15361 agttatgaaa aaatggcaag aaaaagggat caaaccatct gtgatcgtag ttgaccctcc
    15421 acgaaaagga ttagatcatc attttattga atcagcaatc gaaatgtcac cagaacgaat
    15481 cgtctatatc tcatgtaatc cggcaacttt tgcaagagat gctaagttat ttgcagaagc
    15541 aggttatgcc gtgaagaaag ttcagcctgt agatcttttc ccgcaaaccc atcatatcga
    15601 attagtcgct tcattcgtta aaggagaata aaaatggacc aaaaagaaaa aatgaaagaa
    15661 ttgatcaaaa agaaacaagg tggaggccga tccaaacaaa tggaaacacc taagaatgat
    15721 cgtaaaaaca tgagaaaagg ccctaaaatc tttaataaat agacgtgcta aagaaagact
    15781 aagaagtgtt aacttttgtt attgtttctt agtcttttta
//
LOCUS       DANKHL010000053        15812 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000053 DANKHL010000000
VERSION     DANKHL010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.055"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            68..751
                     /locus_tag="PW101_001186"
     CDS             68..751
                     /locus_tag="PW101_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HDL2513598.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="PW101_001187"
     CDS             753..1589
                     /locus_tag="PW101_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL2513599.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="PW101_001188"
     CDS             1600..3354
                     /locus_tag="PW101_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2513600.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLSFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="PW101_001189"
     CDS             complement(3630..3971)
                     /locus_tag="PW101_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2513601.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="PW101_001190"
     CDS             complement(4039..6210)
                     /locus_tag="PW101_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL2513602.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6379..7278
                     /locus_tag="PW101_001191"
     CDS             6379..7278
                     /locus_tag="PW101_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2513603.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="PW101_001192"
     CDS             complement(7349..8206)
                     /locus_tag="PW101_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2513604.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="PW101_001193"
     CDS             complement(8191..10893)
                     /locus_tag="PW101_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDL2513605.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="PW101_001194"
     CDS             complement(10906..12195)
                     /locus_tag="PW101_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HDL2513606.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12345..13391
                     /locus_tag="PW101_001195"
     CDS             12345..13391
                     /locus_tag="PW101_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2513607.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            13393..15546
                     /locus_tag="PW101_001196"
     CDS             13393..15546
                     /locus_tag="PW101_001196"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HDL2513608.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         4491 a         3303 c         2919 g         5099 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgag tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata aaaccttctt tctatttgat atatctttaa agattatact
    12241 tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact ttttcatcca
    12301 ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgact caaccattgt
    12361 accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta cctgtagaca
    12421 gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga atcacttcca
    12481 aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc gggaaaggca
    12541 gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa acacgacgga
    12601 ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc aagggattga
    12661 accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac tttctacaaa
    12721 ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat gctcttgata
    12781 cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct gtcgtcatat
    12841 tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat ttccaaggcg
    12901 gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct tatttgtttg
    12961 gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt gaagcattgg
    13021 acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca ttagatgaat
    13081 tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac gatgttgtcg
    13141 ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa gatttttcaa
    13201 ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg acaaccatcg
    13261 cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact tggttagaga
    13321 caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga caatcaacaa
    13381 aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc gaatcagcca
    13441 aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat gaattatttt
    13501 gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga ccgcgttttt
    13561 caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag tcatatgctg
    13621 tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca aagttcttac
    13681 cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt acgttatggc
    13741 atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga ttacagcaaa
    13801 aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt tatagatcct
    13861 tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga tttttctttt
    13921 tattttatat tgagatttga atatccttta acaattgatg agttagctgt ttccaacaaa
    13981 aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat ccgatttggg
    14041 acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca aaaccaaagc
    14101 aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga tcgtatacag
    14161 ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata tcgggtgttt
    14221 ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca ctatgatacg
    14281 actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg ggatacgtat
    14341 aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga aatgctggaa
    14401 ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc tcctgatgaa
    14461 cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc tgtaaaagat
    14521 atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc tacggttcaa
    14581 agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata tggctatgtt
    14641 cctaatcact accacgaatc agtcaatcac acattagact atgcttatag cgatttttgt
    14701 attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata tcagcagcaa
    14761 tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc aaaagataaa
    14821 gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta tgcagaagga
    14881 agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat cggaaaatat
    14941 ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc cccaacgttt
    15001 gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc gatagaattt
    15061 ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt cagttatata
    15121 ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac cttcgacgat
    15181 tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg gtatatcttc
    15241 aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat cggtatgcca
    15301 ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat tgaagcaacg
    15361 cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacag agaatatacg
    15421 aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta ccaattaggg
    15481 atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt atcaaactat
    15541 aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa
    15601 aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa gcttttgaac
    15661 accgtttatt gagaggttgt gaaatcagcg ttctgacctg agtattagag agacaaatgg
    15721 caaaatagtt cctcatattt tgccattttt tgagctaata ccgagggtca gctgattgaa
    15781 caccatttag tggagaggtc gtgaaaaagc tg
//
LOCUS       DANKHL010000054        15350 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000054 DANKHL010000000
VERSION     DANKHL010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.056"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="PW101_001197"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="PW101_001197"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDL2513609.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2476)
                     /gene="purK"
                     /locus_tag="PW101_001198"
     CDS             complement(1352..2476)
                     /gene="purK"
                     /locus_tag="PW101_001198"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDL2513610.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="PW101_001199"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="PW101_001199"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDL2513611.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3373..4680)
                     /locus_tag="PW101_001200"
     CDS             complement(3373..4680)
                     /locus_tag="PW101_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDL2513612.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4683..5264)
                     /locus_tag="PW101_001201"
     CDS             complement(4683..5264)
                     /locus_tag="PW101_001201"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL2513613.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5380..5477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5836..7128)
                     /locus_tag="PW101_001202"
     CDS             complement(5836..7128)
                     /locus_tag="PW101_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2513614.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7125..7748)
                     /locus_tag="PW101_001203"
     CDS             complement(7125..7748)
                     /locus_tag="PW101_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513615.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7751..8587)
                     /locus_tag="PW101_001204"
     CDS             complement(7751..8587)
                     /locus_tag="PW101_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2513616.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8604..9491)
                     /locus_tag="PW101_001205"
     CDS             complement(8604..9491)
                     /locus_tag="PW101_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2513617.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9507..10790)
                     /locus_tag="PW101_001206"
     CDS             complement(9507..10790)
                     /locus_tag="PW101_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2513618.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11473..12156)
                     /locus_tag="PW101_001207"
     CDS             complement(11473..12156)
                     /locus_tag="PW101_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDL2513619.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12174..12791)
                     /locus_tag="PW101_001208"
     CDS             complement(12174..12791)
                     /locus_tag="PW101_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL2513620.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12801..13625)
                     /locus_tag="PW101_001209"
     CDS             complement(12801..13625)
                     /locus_tag="PW101_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDL2513621.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13618..14178)
                     /locus_tag="PW101_001210"
     CDS             complement(13618..14178)
                     /locus_tag="PW101_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513622.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14352..15308
                     /locus_tag="PW101_001211"
     CDS             14352..15308
                     /locus_tag="PW101_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2513623.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
BASE COUNT         4509 a         3421 c         2630 g         4790 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat
     3121 aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa
     3181 gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc
     3241 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
     3301 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
     3361 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
     3421 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
     3481 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
     3541 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
     3601 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
     3661 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
     3721 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
     3781 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
     3841 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
     3901 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
     3961 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
     4021 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
     4081 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
     4141 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
     4201 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     4261 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     4321 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     4381 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     4441 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     4501 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     4561 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     4621 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     4681 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     4741 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     4801 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     4861 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     4921 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     4981 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     5041 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     5101 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     5161 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     5221 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     5281 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     5341 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     5401 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     5461 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     5521 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     5581 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     5641 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     5701 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     5761 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     5821 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     5881 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     5941 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     6001 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     6061 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     6121 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     6181 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     6241 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     6301 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     6361 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     6421 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     6481 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     6541 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     6601 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     6661 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     6721 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     6781 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     6841 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     6901 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     6961 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     7021 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     7081 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     7141 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     7201 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     7261 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     7321 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     7381 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     7441 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     7501 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     7561 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     7621 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     7681 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     7741 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     7801 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     7861 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     7921 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     7981 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     8041 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     8101 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     8161 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     8221 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     8281 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     8341 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     8401 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     8461 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     8521 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     8581 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     8641 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     8701 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     8761 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     8821 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     8881 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     8941 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     9001 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     9061 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     9121 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     9181 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     9241 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     9301 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     9361 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     9421 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     9481 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     9541 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     9601 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     9661 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     9721 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     9781 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     9841 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     9901 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     9961 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
    10021 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
    10081 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
    10141 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
    10201 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
    10261 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
    10321 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
    10381 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
    10441 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
    10501 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
    10561 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
    10621 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
    10681 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
    10741 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
    10801 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
    10861 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
    10921 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
    10981 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
    11041 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
    11101 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
    11161 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
    11221 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
    11281 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
    11341 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
    11401 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
    11461 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
    11521 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
    11581 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
    11641 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
    11701 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
    11761 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
    11821 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
    11881 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
    11941 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
    12001 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
    12061 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
    12121 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
    12181 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
    12241 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
    12301 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
    12361 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
    12421 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
    12481 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
    12541 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
    12601 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
    12661 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
    12721 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
    12781 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
    12841 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
    12901 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
    12961 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
    13021 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
    13081 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
    13141 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
    13201 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    13261 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    13321 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    13381 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    13441 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    13501 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    13561 aaaataacat agcccattgc tgcgatcata atcacaactg ctccaaatcc taaaaattta
    13621 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    13681 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    13741 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    13801 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    13861 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    13921 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    13981 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    14041 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    14101 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    14161 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    14221 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    14281 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    14341 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    14401 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    14461 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    14521 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    14581 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    14641 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    14701 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    14761 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    14821 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    14881 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    14941 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    15001 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    15061 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    15121 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    15181 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    15241 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    15301 atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa
//
LOCUS       DANKHL010000055        14892 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000055 DANKHL010000000
VERSION     DANKHL010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.058"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            227..1186
                     /locus_tag="PW101_001212"
     CDS             227..1186
                     /locus_tag="PW101_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2513624.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMRGKRKPNGHQEKRGKQQYQRSIHDRSDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            1508..1678
                     /locus_tag="PW101_001213"
     CDS             1508..1678
                     /locus_tag="PW101_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513625.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            1692..2294
                     /locus_tag="PW101_001214"
     CDS             1692..2294
                     /locus_tag="PW101_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2513626.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            2310..2987
                     /locus_tag="PW101_001215"
                     /pseudo
     CDS             2310..2987
                     /locus_tag="PW101_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            3298..3462
                     /locus_tag="PW101_001216"
                     /pseudo
     CDS             3298..3462
                     /locus_tag="PW101_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898188.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
     gene            3499..3795
                     /locus_tag="PW101_001217"
     CDS             3499..3795
                     /locus_tag="PW101_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HDL2513627.1"
                     /translation="MSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTNSASGVLRAN
                     EIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLKEKVVR"
     gene            3796..4212
                     /locus_tag="PW101_001218"
     CDS             3796..4212
                     /locus_tag="PW101_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HDL2513628.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            4232..5776
                     /locus_tag="PW101_001219"
     CDS             4232..5776
                     /locus_tag="PW101_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2513629.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            5919..6143
                     /locus_tag="PW101_001220"
     CDS             5919..6143
                     /locus_tag="PW101_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2513630.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            6158..7027
                     /locus_tag="PW101_001221"
     CDS             6158..7027
                     /locus_tag="PW101_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL2513631.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            7008..7742
                     /locus_tag="PW101_001222"
     CDS             7008..7742
                     /locus_tag="PW101_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2513632.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            7775..8683
                     /locus_tag="PW101_001223"
     CDS             7775..8683
                     /locus_tag="PW101_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HDL2513633.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            8680..9222
                     /gene="sat4"
                     /locus_tag="PW101_001224"
     CDS             8680..9222
                     /gene="sat4"
                     /locus_tag="PW101_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HDL2513634.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            9315..10109
                     /locus_tag="PW101_001225"
                     /note="aph(3')-IIIa"
     CDS             9315..10109
                     /locus_tag="PW101_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDL2513635.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            10385..10957
                     /locus_tag="PW101_001226"
     CDS             10385..10957
                     /locus_tag="PW101_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL2513636.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
     gene            11255..12151
                     /locus_tag="PW101_001227"
     CDS             11255..12151
                     /locus_tag="PW101_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2513637.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
     gene            12250..12459
                     /locus_tag="PW101_001228"
     CDS             12250..12459
                     /locus_tag="PW101_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDL2513638.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            12476..12748
                     /locus_tag="PW101_001229"
     CDS             12476..12748
                     /locus_tag="PW101_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2513639.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            12750..13613
                     /locus_tag="PW101_001230"
     CDS             12750..13613
                     /locus_tag="PW101_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2513640.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(13876..14613)
                     /gene="erm(B)"
                     /locus_tag="PW101_001231"
     CDS             complement(13876..14613)
                     /gene="erm(B)"
                     /locus_tag="PW101_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL2513641.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(14738..14821)
                     /locus_tag="PW101_001232"
     CDS             complement(14738..14821)
                     /locus_tag="PW101_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HDL2513642.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT         5279 a         2344 c         3055 g         4214 t
ORIGIN      
        1 ttcggtctgt actgaaatca aggtattatt gggaatccca gcttaaatca tagataccgt
       61 aagggatttt attctttatt taaaactttg caacagaacc acccgcacta tgccattaca
      121 tttatatcta cgcgaattgt aaaattaagt tagaaaaata aaaaggcatt tatggtacac
      181 tcaaattgta tttccgacga aagaaaacaa aggagtgtaa ccataaatga cctacaaaca
      241 tcttaccata gacgaactga caatgataga atcatattat cttcaacata ataagccggt
      301 tgaaatcgct aaccgaatgg gacgtgctat acaaactatt tataatgtag tcaataagtt
      361 caagcaaggc aagactgctc ttgattattg gcaccagtat aaagaaaata agaaaaaatg
      421 tggtagaaaa gtcattcaat tacctgctca tgaagtagat tacattaaag agaaagtcac
      481 tcttggttgg acgcctgacg tcattatcgg gcgaaaagaa aggtctgttt catgcggtat
      541 gagaacactt tatcgtttat tttctaaagg aatatttgat attgacacac taccgatgag
      601 aggtaaaaga aaacccaatg gccatcagga aaaacgggga aaacaacagt atcagcgctc
      661 aatccatgat cgatctgata attatcctga tttcaattct gagtttggtc accttgaagg
      721 tgatacgatc gttggcattc atcataaaag tgccgtcatt actttagttg aaagattatc
      781 taaagtcatt atcacgatta aacccaacgg ccgtaaggca ttagatattg aaactgccct
      841 taatcaatgg ttttctcgct tccctaaaaa cttctttaaa tctattacgt ttgactgtgg
      901 aaaagaattt tctaactgga aagccattag taaccaacat gatattgata tatattttgc
      961 ggaccctgga acaccttctc aacgcccatt aaacgagaat tctaacggga ttctgcgtcg
     1021 taatggactg ccgaaatcaa tggattttag aaaagtgaat cagacattta tttccagtgt
     1081 cagcaatcaa cgtaatcata ttccaagaaa atcattgaat tacagaacac caatcgaaat
     1141 atttttgagc tatgtacaag aagcatttta ttctagctta atttgacaaa tcatactatg
     1201 atacgtgttt gtttttctgt gctgtttagt gaatgattag cagaaatata gagagtaaga
     1261 ttttaattaa ttattagggg gagaaggaga gagtagcccg aaaactttta gttggcttgg
     1321 actgaacgaa gtgagggaaa ggctactaaa acgtcgaggg gcagtgagag cgaagcgaac
     1381 acttgatctt ttaagttgct atctttttaa ggtcaataga gtatacttat ttgtcctaat
     1441 atggttttag cagtataatt gacttggtga ataggtcatt taagttgggc ataataggag
     1501 gagtaaaatg aaaaaattta tttatcgagt tttagaaaat gacgaagtgg tggctatttt
     1561 taatgagcaa caatatgcgc aagattttat cgcttacgaa aagacaattt ctgataagca
     1621 atttgaaatt gaaaaagtag atatagctga ttggttattg caaccgagag aattttagag
     1681 gttggttgaa aatggctaaa attggttatg cacgtgtcag tagcaaagaa cagaacttag
     1741 atagacaact gaaagcgtta gagggcgttt ctaaggtctt ttcagacaaa ttaagcggtc
     1801 aatcggtcga acgcccacaa ttacaagcta tgcttaacta tattcgtgaa ggggatatag
     1861 ttgttgttac tgaattagat cgattaggac gaaataataa agaattaaca gaattgatga
     1921 atcaaattca aattaagggg gcaaccctgg aagtcttaaa tttaccctca atgaatggta
     1981 ttgaagatga aaatttaaga cggctgatta ataatttagt gattgaattg tataagtacc
     2041 aagcggaatc tgaacgcaaa cgaattaaag aacgccaagc ccaaggaatt gaaattgcta
     2101 agaaaaaagg aaaattcaaa gggcgacaac ctaaattcaa agaaaatgat ccacgtttac
     2161 aacacgcttt cgatttattt ttgaatggtt gttccgataa agaagttgaa gaacaaactg
     2221 gaattaatcg ccgaacgttt agaaggtatc gatcaagata caacgtgaca gtagatcaaa
     2281 gaaaaaaaca atgaaaagag ggatagttaa tgagtacggt tattttagct gaaaaaccaa
     2341 gccaggcatt agcctacgca agtgctttaa aacaaagcac caaaaaagac ggttattttg
     2401 agatcaaaga cccactattt acagatgaaa cgtttatcac ctttggtttt gggcatttag
     2461 tcgaattagc agaaccaggt cattataacg aaaagtggca gaactggaaa cttgaatctt
     2521 tgccgatttt tcctgatcga tacgattttg aagttgcaaa agataaggga aagcagttta
     2581 aaattgttgc tgaacttctc aaaaaggcaa atacaattat tgtcgcaaca gatagcgaca
     2641 gagaaggtga aaatatcgcc tggtcgatta tccataaagc aaatgccttt tctaaagata
     2701 aaacgtataa aagattatgg atcaatagct tagaaaaaga tgtgatccga agcggttttc
     2761 aaaatttgca accaggaatg aattactatc ccttttatca agaagcgcaa acacgccaaa
     2821 ttgccgattg gttgatcggc atgaacgcaa gccctttata taccttgaat ttacaacaaa
     2881 aaggcgtaca aggtacattt tcattaggac gtgtacaaac accgacttta tatctcattt
     2941 atcaacgcca ggaagcaata gaaaacttta aaaaagaacc ttttttcctg aataattcat
     3001 agttttacta atagtctttc ctaacattga tttaatagtc tttaaagtat tctgcttcat
     3061 cacccattag gtgatgaaaa aagaacccct ttctgattta gcggacaaat actttgacct
     3121 aatctcagaa gatataaaag aaaaaataat aaaatatccg atttatactt tgtataaaga
     3181 actaaagtag ttagcaaatc atttacagca aatcactatg ttccacatac cattttacat
     3241 acttacctta tccacgacac atgttgagat agtatcgaag ataaagaaag ttgctccatg
     3301 ttgtttttgc gagatgggga aatcaaagcc acattgacaa ccatgatgaa taagcttgct
     3361 ttcagtcata agctaatcct agaaccactt ttcaagtcag ttagccaaat cgatgaagaa
     3421 agcgaccgtg aaagaatgga cgctattgat aagctaatgg agcaactatt ggaagaacgc
     3481 aataccctta ttgcactgat gtccaaaggt ttcctagaac ctgctctttt taatcaggaa
     3541 cgaaatgttt tagatagtga gattaaaaat cttactaccg aaaaaacaaa cctggtaacg
     3601 aactctgcga gtggggtttt gcgagcaaac gagataaagg acctcattaa ttatgtgtca
     3661 gcagataatt ttaatggtga ctacacggaa gaactatttg aagaatttgt agtgaacatc
     3721 attgtaaatt ccagggatga gctgacattc aatttgaaat gcggtctttc cctgaaagaa
     3781 aaggtggtga gataaatggc atatattcca tatggataca aaattcaaga tggagtggtt
     3841 actgtcgatg aaaaggcagc aggtcaagta aaggtattct ttgagaaata catatcagga
     3901 ctatccctta cagtggctgg cgaacaggca ggtattgaaa agacacattc tgtgatgggg
     3961 cgcattttga aaaacgtcaa ctaccttgga aatgatacgt atccagcaat tattgataaa
     4021 gagatatttg ataaagctga agaagttaga gataaacgtg caaaggattt aggacgagtg
     4081 gtagagcttg ccgctttcac ctctccccct cccaaagaac gattcaaaat gaaaaaggca
     4141 gataataaga tgccagtgga tccttttgaa cgagcagaat acttatacag tctgatagaa
     4201 agcgaggaat aaagtgacag agaaaaatat aatggttatt cctgctcgta aaagagtagg
     4261 aagtacagcc gcaaaagaaa agataaagaa acttcgtgtt gctgcctatt gccgtgtttc
     4321 tacagaaaca gaagaacaaa attctagcta tgaggttcag gtctcacact atacagagtt
     4381 tataaagaaa aataatgaat gggagtttgc tggcatcttt gcagatgatg gtatctctgg
     4441 tacgaacact aaaaagcgtg acgaatttaa tcgcatgatt gcagagtgta tggacggtaa
     4501 catcgacatg gttattacaa aatccatcag ccgatttgcg cgtaacaccc tagactgcct
     4561 tcaatacatt agacagctca aggataagaa catatcagtt tattttgaga aagagaacat
     4621 caacaccacg gatgccaaag gtgaggtttt gctgactatt atggcatctt tggcacagca
     4681 ggaaagccag agcctttcac aaaacgttaa gcttgggcta cagtaccgat accaacaagg
     4741 aaaggtgcag gtcaaccata agcgatttat gggctacacg aaagatgaag atggaaattt
     4801 aactattgtc cccgaagaag ctgagattat caaacgcatc taccgagaat accttgaggg
     4861 tcagagtcta gtgggcattg gtcgagccct tgaaaaggat ggtattttaa cagcagcggg
     4921 aaaaccaaga tggcgaccag aatcagttaa gaagatactt caaaacgaaa aatacatcgg
     4981 agatgccctt ctgcaaaaga ctgtcacagt agattttctg accaagaaac gagtgaaaaa
     5041 tgaagggcat gttccccaat attatgttga aaatagccat gaagcgatta ttcctaaaga
     5101 gttatttttg caggcgcaag aagaaattca tcggagaagc aatatctaca caggagaagg
     5161 caagaacaaa agaatttaca gtagcaagta cgctttaagt gccatcacct tctgtggaga
     5221 ttgtggcgat atttatagac gggtctattg gaatatccac ggaagaaaag aatttgtctg
     5281 gcgatgcgtg actagaatcg agcaaggtcc tgaagtttgt aagaaccgaa ccgtaaaaga
     5341 agatgaactt tatggtgcag taatgactgc gattaataag ctacttgcag gtgggaacaa
     5401 tatgataaag acactggaag agaatattca tgctgtgatt ggcgaaacca cagaatacca
     5461 aatttcagag attaacaact cactagagga aaaacaaaaa gaactaatca agctggcgaa
     5521 caagggtcaa gactatgacc acttagcaga tgagattgat gagctgagag acaagcgaca
     5581 gatcctttta gtagaagatg cctccctcag tggcgagaac gagcgaatca atgagctgat
     5641 tgaatttatc cgcaagaaca aattccgcac cttagaatat gatgataagc ttgtaaggaa
     5701 gataatccag agcgttacag tctatgaagg ccacttcgtc atagccttca aatctggcat
     5761 cgaaatggaa atatgaaaac cagaagtaaa tagcccatga ctctacagta gagttgtggg
     5821 ttttcttttg cttgctgatt ataataatat tttaataact attgtgttta tcaaccaaat
     5881 ggtttataat attcttagcg taaacttgga ggtgctgtat gactacggca gaaatgatta
     5941 aagaactgtg tgagcaaatg aatataagtg tttccgaact tgctagacgt attggccaga
     6001 ctccacagaa tttcaataaa aaattacaac gagaaacagt aaccttggat gagttgaagg
     6061 ccattgctga tgtgctaggt gtcaagtttg tgcaggcatt tattttacca gatggagaag
     6121 aaataaaaat atctaacgaa taaaggagga tgtttatatg gtaggtaatg ttattcaaat
     6181 agtaacagag aaattatcat ctttgccttt tatagaaggc atcgtattag ggggctcacg
     6241 tgcaagaggc acccatacag aaaattctga tatagatatc ggaatctatt acaattcaga
     6301 ctcatttgac ctgacagcga ttaatcaaat tgctacagag ttggatgatg agaatagaaa
     6361 caaccttgtt gtacctcccg gagcgtgggg tgattgggtt aatggcggtg gatggttagt
     6421 tataaacggg tgtcatgttg acttgatatt acgtgatata aaacgggtgg aacaaataat
     6481 caaagatacg gagcaaggaa ttgttactgc caattatcag accgggcatc cccatggtta
     6541 tataagtgct atgtatcgtg gagaattagc gattagtaaa atactatatg ctaagaacga
     6601 aagcttatgc gaattaaaaa agcaggcaga aatttaccct actgctctaa agaaaagctt
     6661 gatgaacttt tttatatttg aagctgaatt ctctttaatg tttgtaaaag caaatgcggg
     6721 ggcagaggat aaatattata ttgcaggcca tgtttttcgt attatttcat gcttaaatca
     6781 agtactattt gcatgtaata atgcttattg cattaacgaa aagaaagcta taaaactgct
     6841 tgaaactttt gaatataaac ctgaaaaata tgccgaaaga gttaatcata tttttgaagt
     6901 actcggtctt tctctttttg aatgctacga catgaccgag aaactttata aagaagtgaa
     6961 tgaaattgta tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca
     7021 aatatgatga taatatattt tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac
     7081 tggctggtgc gggagaatgg gagactttga aaaagatgct acctgatttt aagggtaagc
     7141 gtgtgcttga tttaggatgc ggctatggat ggcactgtat atatgcgatg gaaaacggtg
     7201 cttcctctgt agtaggtgtt gatatttctc ataaaatgct cgaagtagca aaaggaaaaa
     7261 cccattttcc acagattgaa tatgaatgct gtgccataga agatgtggat ttcccagagg
     7321 agagctttga tgtaatacta agttcgcttg cgtttcatta tgtagcagac tatgagaatt
     7381 taataaaaaa gatatatagg atgctgaagg ctggtggcaa tttagttttt acagttgaac
     7441 atcctgtttt tactgctcat ggaacacaag actggtatta taacgaaaaa ggagaaatac
     7501 tgcatttccc ggtggacaat tattattatg agggcaaacg gacagctatg tttttggaag
     7561 aaaaggttac aaaatatcat agaacactga ccacatatct aaatacactg ctttcaaata
     7621 gttttataat aaatcagatt gtggagccac agccgccaga gaacatgatg gatattccgg
     7681 ggatggcgga tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa aagaagatgt
     7741 aataatatag aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga
     7801 tttagtactt tctttagcag aacaggatga acgtattcga attgtgaccc ttgaggggtc
     7861 acgcgcaaat attaatatac ctaaagatga atttcaggat tatgatatta catattttgt
     7921 aagtgatata gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat
     7981 aatgatgcaa aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta
     8041 tcttatgcta tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt
     8101 agataattac ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat
     8161 taaaagggac atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga
     8221 gtatgatgat tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg
     8281 ccgtaaggaa attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct
     8341 gagaatgata tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa
     8401 ctataagttt attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta
     8461 ccggatggat tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag
     8521 ggcggtatcc ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa
     8581 tataacaaaa tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga
     8641 tagcacatat gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca
     8701 gggcacctga aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata
     8761 ccgaggtatg aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta
     8821 aaaagctacc aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt
     8881 gacaatactg ataagataat atatctttac taccaagacg ataaatgcgt cggaaaagtt
     8941 aaactgcgaa aaaattggaa ccggtacgct tatatagaag atatcgccgt atgtaaggat
     9001 ttcagggggc aaggcatagg cagcgcgctt atcaatatat ctatagaatg ggcaaagcat
     9061 aaaaacttgc atggactaat gcttgaaacc caggacaata accttatagc ttgtaaattc
     9121 tatcataatt gtggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa
     9181 aacaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa
     9241 ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag
     9301 gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa
     9361 aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg
     9421 gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg
     9481 atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg
     9541 tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg
     9601 tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg
     9661 cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct
     9721 tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt
     9781 gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa
     9841 agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca
     9901 tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg
     9961 acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac
    10021 agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat
    10081 attatatttt actggatgaa ttgttttagt acctagattt agatgtctaa aaagctttaa
    10141 ctacaagctt tttagacatc taatcttttc tgaagtacat ccgcaactgt ccatactctg
    10201 atgttttata tcttttctaa aagttcgcta gatagagttc tatattagag atgtaaagag
    10261 ttttggtgaa gaggtttgtg agtatggctt ttataaccag ggtgcaatga gaagcactaa
    10321 cagcagctag cttaagaacg cataggatat actttttatg gaagtcacag aggaggaata
    10381 aaatttgaac aggattaaca gagtaacctc tattcttatt cagctgcaat caaaaaaaat
    10441 aattcctgcc aaagaaattg cacagcgatt taatataagc ttaaggacag tttacagaga
    10501 tatccggaca cttgaagaag ccggaatacc aattggatct gaggccggaa aaggatattt
    10561 tcttgtagag ggcttcctac ttccgccggt aatgtttaca gcggcggaag tgggtgcatt
    10621 gattacagca gggaaatttt taaattgcca tggagatgaa tcatttataa aggattttga
    10681 ttcagctatg tataaaatca aatctatttt gaagcatggt gaaaaaaact atgcacagga
    10741 gttggagaac agtattaacg tgtacagcac atctggacag aaaaatacat tggcggataa
    10801 cgttattgca gcaattcaga ctgcaatctg caataaaagg gtaatatcaa ttcaatatcc
    10861 tgcatcagga gggcaagaac cggaaagcag aatgatagag cctatatcac tggggtttta
    10921 tgaacagaac tggtacttaa tcggttttgc aggctaagaa atgaatatag gaattttcgt
    10981 gtggatcgga ttaaaagcat ttgcatagaa gaagagctct gtcaaagcca tgatggctct
    11041 ttggaacaaa tattaaaaca aatgctttca tataaaaaac tatatgccct acaatgccgt
    11101 tgtaaaaatt ggggaaaagg gatatattga ttttatatct ttctcaaaat aaatataaaa
    11161 gccctttaaa gagggctttt atatattaat cacaaatcac ttatcacaaa tcacaagtga
    11221 ttaatcacaa atcacttgtg atttgtgatt cttaatgata caatattact atacaaaaaa
    11281 agaatggggc gtagttatgg agaaggaaaa actaaaaata cttgaagaat taagacgtat
    11341 tttaaacaat aaaaatgaag caattattat cttgaataat tactttaaag gtggtgttgg
    11401 aaagtccaaa ttatcgacta tgtttgctta cttgacagac aaattgaatt taaaagtttt
    11461 aatgatcgat aaggacttac aggcaacatt gacaaaagac ttagcaaaaa cttttgaggt
    11521 agaattgcca cgtgtcaatt tttatgaagg cttgaaaaat ggaaacttgg cttcttctat
    11581 tattcatttg actgataatt tagacttgat ccctggcacg tttgatttga tgttactgcc
    11641 aaaattaact cgctcatgga cgtttgaaaa tgaaagtaga ttgcttgcta ctcttttagc
    11701 acctttaaaa agtgactatg atcttattat tattgatact gtaccaacgc caagcgttta
    11761 tacaaataat gcaatcgtgg cgagtgatta cgttatgatc cctttacaag cagaagaaga
    11821 aagtacaaac aacattcaaa actatatttc ctatttgatt gatttgcaag aacagtttaa
    11881 ccctggacta gatatgattg gttttgttcc ttatttagtt gatacggaca gcgcaacgat
    11941 aaaatcaaac ctggaagaac tgtacaaaga acataaagag gataatttgg ttttccgaaa
    12001 tattatcaag cgaagtaata aagtaagtac ttggtctaaa aatggcatta cagaacacaa
    12061 aggctatgac aaaaaagttt tgtctatgta tgagaacgta ttttttgaaa tgattgagcg
    12121 aatcattcaa ttagaaaatg aaaaagaata gaatcacaaa tcacaagtga ttaatcacaa
    12181 atcacttgtg atttgtgata ggtgatgata aaataaatag taagaagaaa tagaaagaag
    12241 tgagcgatcg tgggaaattt aggcgcacag aaagcaaaac gaaatgatac gccaatcagc
    12301 gcaaaaaaag atataatggg ggataagacg gttcgtgttc gtgccgactt gcaccatatc
    12361 ataaaaatcg aaacagcaaa gaatggcgga aacgtaaaag aagttatgga tcaagcctta
    12421 gaagaatata tacggaaata tttacctgac aaactttaaa aggagtgaaa atgaaatggc
    12481 agttacgtat gaaaaaacat ttgaaattga aatcattaat gagttatcgg caagtgttta
    12541 taatcgggta ttaaattatg ttttgaatca tgaactagat actaaaaata ctcgtttact
    12601 agaagtgaat cttttaaatc aattagaagt ggcacaagaa gttgatttat ttcaacaacc
    12661 atttgaagaa ttacaagcta ttcatgagta ttggcggtca atgaatcaat attcaaaaca
    12721 aattttgaat aaagagaaag tggcttaaca tggcgaatat tactgacttc accgaaaagc
    12781 aatttgaaga tcgtttagaa aagaatgttg aacgactaac taaaaataga ctagcggttg
    12841 aatcgccaac cgctttttta cttggtgggc aaccagggtc agggaaaacc agtttgcgat
    12901 cagcaatttt tgaagaaaca caagggaatg ttattgtcat tgataatgat acctttaaac
    12961 aacagcaccc aaattttgac gaattagtga aactctatga aaaagacgta gtaaaacacg
    13021 ttacccctta ttcgaatcgc atgacagaag cacttattag ccgtttaagc gatcaaggct
    13081 ataatttagt gattgaagga acaggacgaa caacagacgt tcccattcaa acagccacaa
    13141 tgcttcaatc taaaggttat gaaacaaaaa tatacgtcat ggcagtaccg aaaattgaat
    13201 catacttagg aacgattgaa cgatatgaaa ccatgtatgc agatgatcca atgacagcta
    13261 gggcaacacc aaaacaagcg catgatattg ttgtcaaaaa cttaccgacc aatttagaaa
    13321 cccttcataa aacgggctta tttagcgata taaggcttta caatagagaa ggagtgaaac
    13381 tctattcaag cttagaaacg ccttccatta gtccgaaaga gaccttagaa agagaattaa
    13441 atcgtaaagt atcagggaaa gaaattcaac cgactttgga gagaatagag caaaaaatgg
    13501 ttcaaaatca acaccaagaa acccctgaat ttaaagcaat tcaacaaaaa atggaaagct
    13561 tgcagctacc tacaccacca atacccaaaa cacctaaact tccaggtatt taaaccttaa
    13621 aaaaggaaaa gagtagttac caaaaaacgg taactactct ttttttataa aaacatttcc
    13681 tcatgttggg gtcccgagcg cctacgagga atttgtatcg ataagaaata gatttaaaaa
    13741 tttcgctgtt tttttctaat ttcattttga ggtgaattaa ttggtagtta ttagaggtgc
    13801 cctataccta ataatttatc tacattccct ttagtaacgt gtaactttcc aaatttacaa
    13861 aagcgactca tagaattatt tcctcccgtt aaataataga taactattaa aaatagacaa
    13921 tacttgctca taagtaacgg tacttaaatt gtttactttg gcgtgtttca ttgcttgatg
    13981 aaactgattt ttagtaaaca gttgacgata ttctcgattg acccattttg aaacaaagta
    14041 cgtatatagc ttccaatatt tatctggaac atctgtggta tggcgggtaa gttttattaa
    14101 gacactgttt acttttggtt taggatgaaa gcattccgct ggcagcttaa gcaattgctg
    14161 aatcgagact tgagtgtgca agagcaaccc tagtgttcgg tgaatatcca aggtacgctt
    14221 gtagaatcct tcttcaacaa tcagatagat gtcagacgca tggctttcaa aaaccacttt
    14281 tttaataatt tgtgtgctta aatggtaagg aatattccca acaattttat acctctgttt
    14341 gttagggaat tgaaactgta gaatatcttg gtgaattaaa gtgacacgag tgttcagttt
    14401 taatttttct gacgataagt tgaatagatg actgtctaat tcaatagacg ttacctgttt
    14461 acttatttta gccagtttcg tcgttaaatg ccctttacct gttccaattt cgtaaacggt
    14521 atcggtttct tttaaattca attgttttat tatttggttg agtacttttt cactcgttaa
    14581 aaagttttga gaatatttta tatttttgtt catgtaatca ctcctgaagt gattacatct
    14641 ataaataaat acagaagtta aacgatttgt ttgtaatttt agttatctgt ttaaaaagtc
    14701 ataagattag tcactggtag gaattaatct aacgtattta tttatctgcg taatcactgt
    14761 ttttagtctg tttcaaaacg gtagatgttt tatctacatt acgcatttgg aataccaaca
    14821 tgacgaatcc ctccttctta attacaaatt tttagcatct aatttaactt caattcctat
    14881 tatacaaaat tt
//
LOCUS       DANKHL010000056        14815 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000056 DANKHL010000000
VERSION     DANKHL010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.059"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(296..1024)
                     /locus_tag="PW101_001233"
     CDS             complement(296..1024)
                     /locus_tag="PW101_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HDL2513643.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="PW101_001234"
     CDS             complement(1002..2576)
                     /locus_tag="PW101_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL2513644.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="PW101_001235"
     CDS             2805..3743
                     /locus_tag="PW101_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2513645.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="PW101_001236"
     CDS             3755..4666
                     /locus_tag="PW101_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2513646.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="PW101_001237"
     CDS             4690..6162
                     /locus_tag="PW101_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2513647.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="PW101_001238"
     CDS             6213..6824
                     /locus_tag="PW101_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2513648.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="PW101_001239"
     CDS             6854..8767
                     /locus_tag="PW101_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HDL2513649.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="PW101_001240"
     CDS             8895..11876
                     /locus_tag="PW101_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HDL2513650.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="PW101_001241"
     CDS             11873..13534
                     /locus_tag="PW101_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HDL2513651.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="PW101_001242"
     CDS             13574..14506
                     /locus_tag="PW101_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HDL2513652.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DANKHL010000057        14784 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000057 DANKHL010000000
VERSION     DANKHL010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.060"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..656
                     /locus_tag="PW101_001243"
     CDS             <1..656
                     /locus_tag="PW101_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513653.1"
                     /translation="IFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNYFYFVFWVAATL
                     LGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSIEKENFDDIDGY
                     DSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLILEIKKTMDEIQK
                     SDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNGKYSKKYRIFI"
     gene            694..1083
                     /locus_tag="PW101_001244"
     CDS             694..1083
                     /locus_tag="PW101_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2513654.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1067..3517
                     /locus_tag="PW101_001245"
     CDS             1067..3517
                     /locus_tag="PW101_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2513655.1"
                     /translation="MMYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHD
                     NFRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSM
                     IGDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEE
                     IRRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKS
                     ETLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQ
                     QQQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSV
                     DELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMG
                     LHKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKP
                     ALASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWK
                     ETLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEK
                     FPVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGS
                     ITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNRE
                     IFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGL
                     KFAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVF
                     EELFHAFDTRPPVQERME"
     gene            3522..5630
                     /locus_tag="PW101_001246"
     CDS             3522..5630
                     /locus_tag="PW101_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2513656.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            5627..6631
                     /locus_tag="PW101_001247"
     CDS             5627..6631
                     /locus_tag="PW101_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDL2513657.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            6650..7561
                     /locus_tag="PW101_001248"
     CDS             6650..7561
                     /locus_tag="PW101_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2513658.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            7748..8035
                     /locus_tag="PW101_001249"
                     /pseudo
     CDS             7748..8035
                     /locus_tag="PW101_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8223..8438)
                     /locus_tag="PW101_001250"
     CDS             complement(8223..8438)
                     /locus_tag="PW101_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513659.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(8697..8864)
                     /locus_tag="PW101_001251"
     CDS             complement(8697..8864)
                     /locus_tag="PW101_001251"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2513660.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            complement(9209..10648)
                     /locus_tag="PW101_001252"
                     /pseudo
     CDS             complement(9209..10648)
                     /locus_tag="PW101_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(11196..11867)
                     /locus_tag="PW101_001253"
     CDS             complement(11196..11867)
                     /locus_tag="PW101_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HDL2513661.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(12216..13667)
                     /locus_tag="PW101_001254"
     CDS             complement(12216..13667)
                     /locus_tag="PW101_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL2513662.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            complement(13779..>14784)
                     /locus_tag="PW101_001255"
     CDS             complement(13779..>14784)
                     /locus_tag="PW101_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2513663.1"
                     /translation="KQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGA
                     GYIGVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPK
                     KYLGRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTN
                     IKDIYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFS
                     IFNYNFYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRISFEKNTKRIVG
                     VQLCSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET
                     "
BASE COUNT         4723 a         2656 c         2734 g         4671 t
ORIGIN      
        1 acattttttc aattattatt gttatatcgt tattgaattt ttttaaaaaa agaaattcag
       61 gaaaggtgtt taactctaat ggaaatgtgt actacaacta tttttatttt gtattttggg
      121 tagcagcaac tttacttggt tatgataaaa ttcagattgc aggaattcct atacacatgc
      181 aatataagtt ggttttgagt ggaatttttt ctgaggtgtt gccagatata tacgatgatc
      241 attacgacag tgatggaaca tgtaaagtaa gcattgaaaa agaaaatttc gatgacatcg
      301 atggatatga ctctgtgaac ttactgatta ttgatactta cgatataaaa atgagtgaat
      361 tatctatgga aaatcaaaca tatcctacga taattgttag agggaattca atagatggag
      421 ttcgtaaagt taatagatct ttaatcttgg aaataaaaaa aacaatggat gaaattcaaa
      481 aaagtgactt taaaaaagta tttgttgctt caacatctaa tccaaaaaat agtatcaata
      541 ttattaattc atcgtttaga tttttcggtc gatcacgtag gtttaaactt tatgttcttc
      601 aaaaggatta tgcaagcaat ggaaagtata gtaagaaata tagaattttt atttaagaag
      661 gaatttttcc ttctttttat tttggaggaa actatgaaaa aaataaaaag ctacaccagt
      721 atttgggcag ttgaaaaagt catttatgcg attaatgact ttcagctgcc ttttcctgtg
      781 acctttaacc agatggcttg gtttgtttta tccttgatgt ttgtcattgt atttgcccat
      841 gtgcctccgc tatccatgat agagggggct tttttaaaat acttgggcat tccgatagcg
      901 gtcacttggt tcatgtcgca aaagaccttt gacggtaaga aaccattggg atttttacga
      961 tcctttatca gctatcactt acggtttaag gtcacttttg caggaaagaa agttaaagaa
     1021 cagaagaaac gatgggacga accaatcacc ttggtaagga gtgtgaatta tgtacccgat
     1081 taagtatatt gaaaataatc ttgtctttaa ccaagaaggc gagtgttttg cttactatga
     1141 attagtgcct tacaattact cctttttgtc acctgaacag aagtatcaag tacatgataa
     1201 ctttcgccaa ttaattgcgc aaaatcgtga aggtaagatc catgccttac aaattgccac
     1261 agaaagtagt attcgtgcca cacaagaacg ttcgaaaaag gaaattaccg gtagactcaa
     1321 agaagtagca aagcaacgaa ttgatttaca gacagatgca ttagtatcca tgattggcga
     1381 tagtcagatt gattatcgct tctttattgg ctttaaactg atttcgacag atgaagaggt
     1441 caatctaaag aatctcagga aatctttctt ttctggactt caagaatttg tctatggagt
     1501 gaatcatcat ttgatgggtg attttgtttc cttatccaat gaagaaattc gtcgctatac
     1561 caagcttgaa aaactaatgg aaagtaaatt ggctcgaaga tttaaagtca aacgagtaac
     1621 gccgagtgac ttgacgtatt taattgaaca tatctatggt gaaaaaggca caccgtttga
     1681 agaatatgaa ttccagctac caaagaaaaa gctgaaatca gaaacgctgg tgaaacgtta
     1741 cgacttatta cgtcccactc gttgtttgat tgaagaaaag cctcggtact tacggatgga
     1801 acatgaaaat catgagtctt atgtagcgta cttaacaatt aataccattg taggggaaat
     1861 ggagtttcct tcctcggaac ttttctatta ccagcaacaa caatttacgt ttccgattga
     1921 tacttcaatg aatgtggaaa ttgtgacgaa taaaaaggcc ctagctaccg ttcgtaataa
     1981 gaaaaaggaa ttaaaagatt tagacaacca tgcctatcaa tctgataatg aaacgaactc
     2041 caatgtatta gatgctttgg attccgtgga tgaattggaa accaccttgg atcagtcgaa
     2101 agaatccatg tataaactaa gctatgtggt tcgagtgagt gctaaatcag tggatgaatt
     2161 aaaacgccgc tgtgatgaag tgctcgattt ttatgacgat accaatgtaa aactagtccg
     2221 gccctttggc gatatgatgg gtctacacaa agaattctta ccctcaagta agcgatacat
     2281 gaatgactat attcaatatg ttacgtctga ctttttggcg ggacttggtt ttggggctac
     2341 tcaaatgcta ggagaattag aaggcattta ctttggctat aacgtagata cggggcgcaa
     2401 tgtttacctg aaaccggcat tggcatcaca aggggtcaaa ggctcagtaa ccaatgcttt
     2461 ggcatctgct ttccttggtt cgttaggtgg ggggaaatct tttagcaaca atctcttagt
     2521 ctattatgcc gtcctttttg gtgggcaagc tgtgattgtc gatccaaaag gagaaagagg
     2581 cggttggaaa gaaacgttgc cagaaattgc ggatgaaatt aatattctta acttgaccag
     2641 taagccagaa aatcaaggct tacttgatcc ctatgtgatt atgaagaaaa agaaagactc
     2701 agaaagtttg gcgattgata tcctgacctt tttaacaggg atttccagtc gggatgggga
     2761 gaagttccct gtgttacgca aagccattcg aacggttacc caaagtgatc agcgaggatt
     2821 attacaagta atcgttgaat tgcgtaaaga ggactcacca gtcgcggaaa atatcgcgga
     2881 tcacatcgag tcctttaccg attatgattt tgcccatttg cttttctcag atggttcgat
     2941 tacccagtcg attagtttag ataagcagtt aaatatcatt caagtagcgg atttggtttt
     3001 accagatgca gaaaccactt ttgaagaata taccaccatg gaattgctga gtgtttccat
     3061 gctaattgtt atttccactt ttgccttgga ctttatccat agcaaccgag aaattttcaa
     3121 gattgtggac ctggatgagg cttggagttt tcttcaagta gctcaaggaa aaacgttatc
     3181 gaataaatta gtgcgtgctg gtcgggccat gaatgccggt gtttacttcg tcacgcaaaa
     3241 cagtgatgac ttgttggatg aacgtttgaa gaataatatt gggctaaagt ttgcatttcg
     3301 cagtaccgat attcatgaaa tcaagaaaac cttggaattc tttggattag ataaagaaga
     3361 tgaaagtaat caaaaatgca tgcgagattt ggaaaatggg caatgcttga tgcaggattt
     3421 gtatggtcga gtaggtgtgg tccaagttca tccagtcttt gaagaacttt tccatgcttt
     3481 tgatacccgt ccgccagttc aagagagaat ggagtgagga gatgaaaaag aaactttttc
     3541 gtatctttgg aattgtttta atcagtttgg tcactctgat aatacttttg agcttagttg
     3601 gaacggtagc tgaggcaaca ggtctggttg atgatacagt tggagccgga aatctttatt
     3661 cccaatattc acttggtaac tatcagttgg atttctttgt ggatagttcg tgggattggc
     3721 tgccttggaa ttggggtgat ggcctaggga agagcgtaat gtatggactt tatgccatta
     3781 ctaattttat ttggacagtt agtctctact tatcaaatgc cacgggctac gtggttcaag
     3841 aagcctataa gctggatttc atttcagata cggctgaaag tattgggaag aatatccaaa
     3901 ccttagccgg cattaccgaa aatggactcc agacctcggg cttttacttt gggtttctat
     3961 tattgatgat tttagctcta ggagtttatg tggcgtatac aggattatta aaacgagaaa
     4021 ctaccaaagc agtccgggct gtcattaatt ttgtgatgat ttttctactt tcgggttcgt
     4081 tcattgcgta tgcgcccacc tatatcacaa aaatcaatga ttttagctca gatgtcagcg
     4141 aagcggctct tagccttgga accgagattg tcgtgccgaa ttcagaaagc caaggaaagg
     4201 acagtgtgga tttaatccga gatagcttgt tttccattca agtccaacaa ccgtggttac
     4261 tcttgcaatt tgatgattcc aatatagaag aagttggcga agatcgtgtt aacaaaatcc
     4321 tgtctgtaag tccagatgaa aataaaggca aggatcggga agaagcggtc aaagcggaga
     4381 ttgaagataa tgacaatgcg aatctcagta ttaccaagac catgaaccgc ttagggaccg
     4441 tggtcttttt ggtgttgttc aatattggca tatccttctt tgtctttctc ctaacaggga
     4501 ttatgttgtt ctcgcaaatt ctctttatta tctttgcgat gtttttgccg attagtttct
     4561 tattgtccat gttgccgact tatgagagtt tgggcaagaa ggcgattatt cgcttgttca
     4621 atacgattat gatgcgagca ggagttactt tggtgattac gactgcattt agcatctcca
     4681 cgatgttttt caatatttcg gcaacctatc catttttcat ggttgctttt ctgcaaattg
     4741 tgacctttgc cggtatctat ttcaaattgg gcgacattat gaatatgttc aatctccaga
     4801 gtaatgatag ccaatcaatg ggaagacggg tcatgcgaaa accgcaaatg ttaatgaatc
     4861 gaacattgcg gcaactcaat cgaaatgtgg gacggacttt ggcttttggt ggtggtgcag
     4921 cagttggaaa taagttggca aaggaacaat caaaatccaa atttaagcca tccggttctt
     4981 ctctacgaaa gaattcacga ttaccgaacg atcacgagat accatctgac ttgaataagg
     5041 aaaagccaat tccaaataat aagaaacaat cccgaatgaa tttggccggg agaaaagtcg
     5101 gcaaggttct ggatacccaa gctttggtga aagataagac caaacaagta aaggatggag
     5161 tacggaatac gccaaccaac ttgaaataca acttccataa aggaattgaa aaaacgaaaa
     5221 aagcaccgga agagtttaag cgaggactgg tccaagaaaa agcaaatcga ggagagttgc
     5281 gagaaaaaca acaacaacgt agagacgaaa aaatagctga aaagcgcaaa gtattgaacg
     5341 aaatagggaa tcctcatggg aaaagaaggg aaaatgttcc gataaaagca aaggtccaat
     5401 ctcaaaagga tagagcacca aagcggattg tgagagcaaa ttcgaatccc gaaatcaaac
     5461 gaaaattcgc tagccaagaa atcatttcaa aaggaataaa ccaatcctca agtaagaaaa
     5521 attcttttca acaagtgaaa cggcggtcta ctctgtccaa acgaaccaat cgaaaagtac
     5581 aaaaaagttt gaaccctcat cccaaaccaa ggtcaggtga gaaaaaatga acgtaaagaa
     5641 attaagtgcc atttcgttaa ctttcctaac aatctctttt atgggtatct tgatgtgtgt
     5701 gggcttattg tttggtgaag acagtgaagg aagtagtagt tcaggtgctc ctcctggggg
     5761 gagtagtgtc tcagaagagg ttttgaaaca ccgtgcaatg gtggaaaaat atgccaaaga
     5821 atcggatata acagagtatg ttccgattct tttagcgatt attcaagtgg aatctggtgg
     5881 cacaatggaa gatgtgatgc aaagttctga gtcagccggt ttaccaccga attcattaag
     5941 taccgaggca tccatcaaac aaggatgtct ctattttgcg tctttggtta agcgttccaa
     6001 agaattaggt tgtgatcaag atagtatcat tcaggcctat aactttggcg gtggttattt
     6061 ggactatgtg gcaaaaaacg ggaagaagca tagttttgct ttagctgaat ctttttcaaa
     6121 ggaaaaatct tgcggtcaaa aaatcgatta ccctaatccc atcgcgaata aggaaaatgg
     6181 tggatggcgc tataactatg gcaatatgtt ttattgctta ttggtgaagc aatacctaac
     6241 tacaacacag tttgacgata aaacagtcca agggattttt gacgaggcgt ttaagtatga
     6301 aggaacggct tatgtatttg gtgggtcaag acccgaaaca ggatttgatt gtagtggctt
     6361 gactcaatgg tgttatgcca aagttggtct gaaacttcct cgggtggctc aagatcagta
     6421 tgatgccatg acccatattg acctaaaaga tgccaaacca ggggatttgg ttttctttca
     6481 ttcgacttat gatgccggta cttatgtgac ccatgtcggt atttatgcag gggagaatcg
     6541 catgtttcat gcaggagatc cagtaggctg gacgaatcta acagaaagct attggcagca
     6601 gcatcttatc ggtgctggac ggtacaaata agagaaaaga ggaaaaagaa tgaagataaa
     6661 aattgaacgg aatcaaaagg aaaagagcct aaaaaagaaa aaagaacgag tcgtatccgt
     6721 gggtaagcat cgaaaaatgg tgcttgccct ttggctttta ttatcttgta gtcttacttt
     6781 tggcgtttat aaaaatttca ccgccattga tcaacataca actcatgaaa aagtggttat
     6841 taaagagaaa gtagtgaata cttctggtat tgaaaacttt acgaaggatt tttcgaaaga
     6901 atatttttcg tggaaaaata acaaagaagc aattgaaaag agaatgaaca acctcgaaca
     6961 gtaccttaca gaagaaggtt tcgctttaag tcaagatatg gttcgagcag atattcccac
     7021 taattccgaa gttcagtcag taaaagtctt agatgttgaa aagagctcgg aggaatttgt
     7081 cgtttcattt ttagtggagc aaaagattac agagggaaaa aaggcacaat cgatttcttc
     7141 tgcataccga gtgacgatct ttgaagatga aaacaggaat tatatagtaa cgagtttgcc
     7201 aactatgatt gcaaaacctg atagggcaaa atacaagaca aaacaagtag aaaatgattc
     7261 gaaaattgat gccaaaacta cagaagaaat cgctgagttt ttggaaacgt tctttaaatt
     7321 atatccatca gcttctgaaa aagaattgga gtattatgtt gaaaatgatg cgatgcgacc
     7381 aatcaatatt gacttaaagt tttcgaagtt agaggatcca gtttacctac aaaacggaaa
     7441 aaaaattcta gtgagtacaa agataaaata ccttgataat gtatcaaaaa ccactagcta
     7501 ctttcagtac aaattaatat tacaaaaaaa tggaaactgg aaaatcattg atgttaaata
     7561 ataatgactc aatctaaatt tatgagaagt tataagtagt ttaacttatt tcaggcagaa
     7621 gtcgcccatc ctctataggt gggtgatgaa tgccgttcgg taactcgaaa accgacaatt
     7681 aagacatagt gcctttttat tttaatccat gtcataatca gtctatataa gaaatggttg
     7741 atataccatg gaattagata caaataatca ttcattattt cttctttgtt accaccttat
     7801 tttagtagtg aaacattgta aacatctcat tgatgatgta atgttagctt atgcaaaaaa
     7861 ctatctttga aagaatatca gaatcacatc acattacctt agttgagtga aatcgtgata
     7921 aagactactt tcatattatg ctcaaagcac aacctaaaac cgaattaaca aaattcagca
     7981 atgcctaagc tcatgtctga taaaatgaga ctttctaagg gtcaaaaatt tcctttgaat
     8041 acgatcatta gcttagataa aagtaaaaac cttagaacta cggggatagc ttggttaata
     8101 agagatatcg ctgttaataa agacataagc ttgcaagtat gctctattcc caagaatctc
     8161 ccacttcaag cactaaggat cgttaaattc agctaagtgg caagtagttc actagacgat
     8221 aattatctcc atatttcaat ctcagctttt ccatttttca tagcaaattc cgttattttt
     8281 atagcatcta aaagattcag cttttccact gatctttcac cacttttcag cttttttatt
     8341 gtgcttaaat tgataccagt accttttgat attgaattgg aagtaaaatc attaagggtt
     8401 ttttttatta aattcatatc cgcaattcca gcgttcataa gaatgaagat tctcctctca
     8461 tagactactt tgaaaatggt actacagtgg ctgtttatat atttcagtag gttactgtag
     8521 tggtcatttt attataatta gaaaaggaac ctaaatcaaa aatgctaaag aaaattttca
     8581 tagtattaaa cctatcatga ctaaagccac ggaattcttg ataaaagagt atacttgcaa
     8641 gcctatgtct ttactaacag cgatatctct tattaaccaa tctatccccg ttgttcctaa
     8701 ggttcttgat gttttctaag gcaatgcttg tatacatagc ccttgatcaa aatattgata
     8761 ctaacgttga cgtttcgatc atgatgtgta cggcaaatag gacaagtcca ttctcgaatt
     8821 gtgaatgact tcttaccatc tgtgtgtcca cattctgaac caatttgact agacggaaat
     8881 catttatcta tcttgatgat ttttttgaca gaagtatcga aaatctttac ataatctggg
     8941 tgttgcattc ttatgcagat gagagcgtta gctccgtatt cataaccagt tgcctctaaa
     9001 acgtagtcca tcggtggata cctagcttgc ggttgccagt ggctttcgca gtcttcttgt
     9061 aatcaaatcg agcatctaat tgagtactag tattttgaag ataatacttg agtttttcgg
     9121 ttttcagaag ttcacttcat cttccatcat ccatggaaat cccactcacc catacgcagg
     9181 cctccaaaaa ttcttcaaca atggtttttc agacattttt ctgaatacct ccttgttttt
     9241 tgatacgcca gatagtcagg aaaaaggcaa caaaacacaa aacaaagctg cctaaaaagg
     9301 taatcttcat tccatataaa aagacatccg gccgatctgc gatatagtta gttactcgtt
     9361 ggcccatctt agcagacatc gcttgatata agaccgtcgt agaaagcgaa atcccgatga
     9421 ccatccccaa atttcgggca aaggaattca tggagcctgc cagtcccaaa ttttcgatag
     9481 tgacagaact cataaccatc gtattatttg gggactggaa caaggcattg ccggcaccca
     9541 tgatggcagt agcgacaatg tacagccaca aaggagagtc cactgacaaa aacatataca
     9601 tgacttgggt taccgacaat aaacccaagc ctactaatac aaaaatctcc gggccaaccc
     9661 ggtcggtcag gtatccgctt aaaggcgccc cgacgaccat catcaaggga aagaccatca
     9721 tcaacagtcc ggcatagcta ggactcaacc cgcgagcatt ttgcagataa aaaggaatca
     9781 ctacattaac aaagaagttg gaagaaaaaa tcaacaaggc tgtaatcaag ctcatggtaa
     9841 aaatcttgtt cttaaaaata ccgaaagaaa tcaatggaga ggctgctttc ttttcaactt
     9901 ttaaaaagct gacaaaggca atcactgaaa ttacgaacag tacaaggggg actggatgga
     9961 aaaagccact ttcttgcccc aaaaagattg ttccaaaaag gctgataatc gtagccgcaa
    10021 tggcagcaaa gccccctcca tcgattgtat ggccgctcat ggtgatatct tttggtaaga
    10081 atttttcagc aatcaacatc gttacgattc ccaccggtac attgatccaa aaaatgtacg
    10141 cccaagaaaa ttgggacaaa atcaagccgc caagacctgg tccagcaatc gagcctaatg
    10201 aaacaagcgc accgattgct cccaatgccc gaccacgttc atgtatggga aagacttccg
    10261 tgatgattcc ggcatttgta gccatagtca tactagcacc aatggcttgt accacccgtg
    10321 cgaacaatta aatacctagc gaatgattga acccacacaa cagcgagccc aagacaaaga
    10381 tcagcgtacc aatccgaaat actttgattt tcccacagct atcgccaatt ttaccaaaca
    10441 gcaacaaaca agaacagaca atcatcagat aaacggacac tacccattcc gcttgattca
    10501 ttggtatgcc catatcatct gagatggtag gtagtgcaat attgacaata ctgccatcca
    10561 atgtggacat gaaggtaaac atagatatag caatcaaaat ccaccagcga tttttttgga
    10621 cttttgcatc ttcttgataa ctatgcataa taggatttcc tttctaatga tcgtgtagct
    10681 aagaacaata taacctatgt ctactgccca acaacttctc gcgactctag ccataggaag
    10741 cttgagtaat ttcgatttgt tattctcatg taaccatgta agtttgagat gctttatttg
    10801 aacttactct atagtaacta gtgataattc gctttacttt cttctagcaa tttctaaaag
    10861 tcgacttcat tgttcttcag attaatagtg tgagctatct cgtgagacgt aagaaaattt
    10921 ccattctact ttttcccaat tttttcatat ttatggtgtt gcacctattt gttttccaat
    10981 ttgcttttca aatacattgc tatgatattc aattccatag gattatacta aatgtcaata
    11041 gtatgaataa tatattatag atgtttatat atcactgtta attattatcc acaataatta
    11101 atgattacct cgatatttgt caatcaaagg ttgaaacaat ttattttcag catcttttct
    11161 aacttgagct gcactcttaa tagaatcata cgatcctaaa tagtacctct tttttttaaa
    11221 gttatatacg caatccattt ctctttagaa cgataaactc ccttaacacc acttgtatta
    11281 tttttcggta attttgacgt tagacactct attactgtcc cttcaacaca tgtccttgtt
    11341 tcttgccttt ttaaacatcc acaactttga gtatgtccaa aaaataagtt actataactt
    11401 atctcacaca tactgccaca ctcacattga caaacccatt ttgttttcca tgaacagtta
    11461 ttgtccaaag gatactttgc agtaagtaat ccgaattttt tattttcaat gtgatgcttt
    11521 tttgatttca aacatccaca gctttttaca tcacctttta gaagtcgctt tgccgatact
    11581 aacttttttc tcccacacag acattgacat tcccaaacaa cagttccttc acttcgcagt
    11641 ttagttggct tgattgcaac taacttacca aaagttttat ttgtcaaatt tttatattta
    11701 tgcctatttt tataatttcc acatccacaa cttttggttc tacctgatag taagtttgat
    11761 cgatatacaa tcgtttcatt cccacagtca cataggtaac gatatttttt atatccatta
    11821 tgagtaatct cctcaacaac agtaaggtga ccatatcttt ccttcactgc acctaccctc
    11881 ccaagaaaga agtaccatat aatttcaatt aacttttaat aaatcagaaa aaaatatttt
    11941 caatttttct tagtctgatt ttatgactaa tttataatac aagactgaac gatacgtaca
    12001 cgaaatcctc aagatagata aattgttttc atctagtaaa acctgctcaa aatatgagca
    12061 taaagacggc aataatacac tcgatattcg ataatggact tgttttattt gtcaaactca
    12121 tcgtaatcga gatatcaatt ccagtctcaa tatcctgatc aaaggtctaa gaatccaacc
    12181 attttcttgt tactttgtca gattgattac cttttttagt ctccgaataa taaggttttc
    12241 ggatcaacta acatgctctg agaaagacta ctatctttgc aaatttccaa atgaaggtgt
    12301 gggccagtac tattcccagt acttcctaca tagccaatta tttgtccttg caaaacatta
    12361 tctccagttt ttactaggta ttgttgctgg tgagcatata acgcggttga tccatcttcg
    12421 tgttgtatca ccacgtaatt tccccaagag tagtgaaact ctgctttaac aaccttacca
    12481 gctttaactg catgaatcgg ctcaccctgt gtagctgcca tatcaagtcc tcggtgaaat
    12541 tcatttccac gatttccaaa caaactggtt attgaataat tatgaatagg cacttggtat
    12601 ccagtcttac tcatttgtgg acctttaact tctttatcat agttggttaa atcataggtt
    12661 ttaattaatt catttagctt caaatagtat tgtgtatctg ttgcataccg tcctgtaagg
    12721 aatttagttg cttctcgata tgttttacta ttagctttta atgctcctgc ataaaaatct
    12781 ttatttccac taattccctt agttaataat ttggcataat cttcaaaact ttcctcatag
    12841 ttttcataca ctctaaattt tgattgggtc gtataaagcg ttccattacc caaatcttcg
    12901 tttgttacca taatgacaaa gtttccatta taagttcctt ttatcccaaa taaattgtaa
    12961 ttcggtgcct gagctaactg gctttgacca ctggcagatt ctaagatcgc ttgtgcaatc
    13021 ataacagaag catataaatc atatttctga ccaatttttc tcgcagactc accaattttc
    13081 ataataaaat tttctacaga ttcatttttg tcaaaatgaa tttcttcatt accagatagt
    13141 attggttgtg attggaaaga ttctgttgat tctatttgct gtggtgtcga aaaatttttt
    13201 gtttgtcctt ccgtttttgg cgcttccttt tttgaagact ctatatggtt tggtcttgtc
    13261 actttcgaag aagttatttc acttgtacta cttgaagttg ttgaagattc ggcacttgaa
    13321 atcgtactac tttcacttga cgaagttgtt gaagactcgg catttgaagt cgtactactt
    13381 tcacttgacg aagttgttga agactcggca tttgaagtcg tactactttc acttgatgaa
    13441 gttgttgaag actcagcatt tgaagtcgta ctactttcac ttgataaagt tgtcaaaagt
    13501 tcactactct ctgctgttac atcggaatta ctcatttcag tcgtctcggt tctttctttt
    13561 gcaattttgt atgttgaatt ttcgcttgcc aacacatgga aaggtgttgc tataaagagt
    13621 tgggacatta atataactgc tgatgcaagt tttaaagatt ttttcatttg atttacctct
    13681 cttttttctc tttaatagtg ttttctatta aaaattatga taaatatttt caatgaattg
    13741 agtaatacag tatatttctt gatttcattt agtaacgatt atgtttcaat gtttaatcct
    13801 gttactgcct ttaaaataaa attatagggt tggctaaaac taggatgaaa taaataatct
    13861 aacattttca atttgtctaa tgtaatttgt tcttctatag caagagaaaa cattgataat
    13921 agtccagata aatcttcttg actacaaagt tgtacaccta ctattctctt tgtatttttt
    13981 tcaaagctaa ttcttaattt tacacgacta ttttttctca aaaaaacagg tctaatgaaa
    14041 tcttctattt ctatataatc tatagactca cttaataatc gtgctgtttt taaattcaaa
    14101 cctgctgcgt aaaaattgta attaaatata gaaaagccat ttgtaaattg ggaaccagaa
    14161 ggtgtaaaat cttgattgtc aatatgttga gttgcaatat aagctgttct tagagcatta
    14221 gaaatagaaa aatcaacact acttctatta atggacgtaa aaaaagttgt agcacaatcc
    14281 ccaatagcat atatatcttt tatatttgtt tgctggtatt cattagttag atatgctcca
    14341 ctaacatctc tcaagagttc ctttttgcca attggactat tagatagaaa tcctatagct
    14401 tgtacgacca tatccataga gtattgggta ccacttgtag tgactatttt ttcaatcttt
    14461 tttcttccta aatatttttt aggactttct gatagacaaa gatgtattcc attttttgat
    14521 agtatttctt caactttatc tgaaaaacta cggtcgtaat aagtcgacaa cactctatct
    14581 gcaacatcga aaagatatac ttctttccct ctttttctta tggcttcagc aatttcaact
    14641 ccaatatatc ctgctccaat aattcctact tttttaatag tattatcatc taaagcttta
    14701 aaaatctctt tagcatttgg aaaattcttt atcgaaaaaa tattagctaa attattcccc
    14761 ggtacatcta acataatttg cttt
//
LOCUS       DANKHL010000058        14624 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000058 DANKHL010000000
VERSION     DANKHL010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.061"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(114..308)
                     /gene="ssrS"
                     /locus_tag="PW101_001256"
     ncRNA           complement(114..308)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PW101_001256"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(380..1666)
                     /locus_tag="PW101_001257"
     CDS             complement(380..1666)
                     /locus_tag="PW101_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDL2513664.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            1804..2475
                     /locus_tag="PW101_001258"
     CDS             1804..2475
                     /locus_tag="PW101_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDL2513665.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            2441..2929
                     /locus_tag="PW101_001259"
     CDS             2441..2929
                     /locus_tag="PW101_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HDL2513666.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            2942..3889
                     /gene="prmA"
                     /locus_tag="PW101_001260"
     CDS             2942..3889
                     /gene="prmA"
                     /locus_tag="PW101_001260"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDL2513667.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            3890..4654
                     /locus_tag="PW101_001261"
     CDS             3890..4654
                     /locus_tag="PW101_001261"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDL2513668.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            4887..7100
                     /locus_tag="PW101_001262"
     CDS             4887..7100
                     /locus_tag="PW101_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDL2513669.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFTYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            7111..7557
                     /locus_tag="PW101_001263"
     CDS             7111..7557
                     /locus_tag="PW101_001263"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDL2513670.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            7728..8987
                     /locus_tag="PW101_001264"
     CDS             7728..8987
                     /locus_tag="PW101_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HDL2513671.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(9076..10023)
                     /locus_tag="PW101_001265"
     CDS             complement(9076..10023)
                     /locus_tag="PW101_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2513672.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            10417..11709
                     /locus_tag="PW101_001266"
     CDS             10417..11709
                     /locus_tag="PW101_001266"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDL2513673.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            11724..13493
                     /gene="aspS"
                     /locus_tag="PW101_001267"
     CDS             11724..13493
                     /gene="aspS"
                     /locus_tag="PW101_001267"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDL2513674.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            13602..14477
                     /locus_tag="PW101_001268"
     CDS             13602..14477
                     /locus_tag="PW101_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2513675.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
BASE COUNT         4794 a         2637 c         3173 g         4020 t
ORIGIN      
        1 tactaaagtg gcgataatca ttaaaatcct aatcatgaac aaatcctcca aaataagcaa
       61 caaatagaaa aagcaatttg ccaagagcaa attgctttat tctttaaaag atggtaatct
      121 ccgttaatgc cgttgtttgt cctgtagtat tgatcccgcg aattcaagca ggtgggtccc
      181 atggtcgtag cttcgaacta ccaaatgata aggcatgtta ccagcagtga cacaagacgg
      241 gtcccaagat atcaataaaa atgttcggtc aacacacaaa gaggacaatt cgaacatcac
      301 agatttccca cctctatagt agcatatgaa tttaaaaaga gcaatgagag agctcgcaga
      361 aacagaaccg ttttcttgtc tagttttttg gaggatgctt tttccactct tttatacggt
      421 aatactgttg acccagagcc tgttcatatt tccctgtatc ttttggctga taatactgag
      481 cattcttcag tttatctggt aaatattgct gattgaccca agcctgatca aaatgatgag
      541 gatactgata acccacgcca cggttgagtg atttcgcacc tttgtaatgg ctatctctca
      601 aatgatcagg aacatctcca gctttgccct ctcgaatatc agcaagtgct gcatctaatg
      661 ccatgtaggc agaattggat tttggcgaca agcaaagatc gacgacgacg tctgctagtg
      721 ggatacgtgc ctcaggcaat cccaattttt cagcagctaa tacagcgttc actgtacgag
      781 ccgcagcagc aggattacct aaacctatat cttcataacc gataaccatc agacgccggc
      841 aaatacttgc cagatcacct gcctcaacta aacgggcgag ataatgaagt gcagcatcca
      901 catcacttcc acgaatcgat ttttgaaaag ccgaaataac gtcataatga gcatctccat
      961 ttttgtcatg ggtaagagct tttctttgga cacattcttc aatgatcgac agtgtaagat
     1021 gaatccttcc ttctttatct gaaggagtcg atagtgtagc cagttccagc ccattcaatg
     1081 cactccgcaa atcgccgttc gttgcacgcg aaaggtgaag aagagcatct tcgtctaact
     1141 ggattgcttg ttgtccaagg ccgcgttctt tatcccttaa agcatgttcg actgccaact
     1201 ggatgtcttg ttctgttaat ggtttgactt caaagatctg tgtacggctg cggatcgctg
     1261 gattgatggt aatataagga ttctcagtag tcgctccgat caagatgatt cggccacttt
     1321 ctaaatgagg aagcagaaaa tcctgttttg ttttatctaa tcggtgtact tcatccaata
     1381 agaggatcac tgtaccactc atttttgctt cttccgctac aacttgtaga tccttttttg
     1441 aatctgtagc ggcgtttagc atgcgaaatg cataattagt agagccggcg atcgcacttg
     1501 cgatactcgt ttttccagta ccaggaggtc catatagaat catggaagag agcatgcgag
     1561 catcgaccat gcggcgtatg atttttcctt ccccgactag atgctgctga ccaacgactt
     1621 catcgatcgt tcgaggtctc atgcgataag ccaatggctg ttgcatagga ttttcctcct
     1681 tcttgttttt acgtccctta attatatcat atttagcaag atgcctagaa agaacagcct
     1741 tctgttgttt tgttttcata gcttatccaa ctaatattgt gtatgataaa gtaggtgaga
     1801 aaaatgacgg ataaacaaaa gatagatgaa tttttttgga ataaaacgac gccagaagtg
     1861 gcacgggatt tattggggat gtatttggag catgacacac cagaaggaag attagctgga
     1921 tatattgtag acgcggaagc atacctaggt ccagaagacg aggccgccca tagctatggt
     1981 ttaagaagaa caccgcgtgt gcgggcaatg tatgaaaaac caggtacgat ctacctttac
     2041 acgatgcata ctcatcggat tttgaacatc attactcagc cagaagggat tcctcaagga
     2101 gtaatgatac gtgcaatcga acccgccgca atgattgatc aaatgagtaa aaatcgtggc
     2161 ggaaaaactg gtccagatat cagcaacggt ccgggaaaat tagtggaagc attagcaatt
     2221 cctcaagaac tttatggtca gtctatagca gatagttcgt tacgcttagt ttttgagaaa
     2281 aaaaagacac caaaaaagat caatgctttg cctagaatcg gtatcccaaa caaaggtgta
     2341 tggacagaaa agccgttgcg ttttgttgtg tcgggaaatc cttatatctc tttacaaaga
     2401 aaaaaccaaa tcgagaaaga ttggggttgg aggaaagaaa atgaaaaaga aggatctatt
     2461 aacatattta gatgaaataa ttgaaaaaca agtgacagac tatgatgtag ctttagattg
     2521 ggatacaaaa aatcatacga tcgagatcgt tgttcgcttg tttgcagaaa ataaagcaca
     2581 atttgagatt gatgatgcag aagggatcgt ttcgcaagaa ccaattatcg aatttgaaga
     2641 cggcgtactt ctttttaatc ctcagaaatc tgtttttgat gaacgcaatt atctagcagt
     2701 aatcccttat gaaggaaaaa aagggttggc aaaatcagta gctgattctt tagtggaata
     2761 tctaaatgaa gtattagctc aagggcaaag cgatttgctt gattttttgg atgaagacga
     2821 tgacgaagct gtattcgagc ttcactggga taatcagcaa ctagcagaat tagtagaagt
     2881 aaaacagcaa aatgaagcag atacttattt accgtaccca agttattaag gagtgacaga
     2941 tatgaaatgg aatgaagtaa aagtagaaac tgaaagtgaa gcagtcgaag cagttgctaa
     3001 tattttgatg gaggcagggg cgagtggtgt cgcgattgaa gattctttag atgttgaaca
     3061 ttttcagtcg gatcaatatg gtgagatttt agataaaaat gattttacat cgctgaaaga
     3121 aggcgcacta gtcatggctt attttccgga aacgactttc ttaccggaaa tcttgccttt
     3181 tatcaaagat cgtatcacac aattacctga gtttggtcta gctattggaa aaaataaagt
     3241 aacagttagt gaagtagagg aaagcaactg ggcaacagcc tggaagaaat actatcatcc
     3301 ggtgcgtatt acgcgaatgc tgacggttgc gccaagctgg gaaaactacc agacagaaga
     3361 tcctctagaa aaaattatca cactagatcc tggtatggca tttggcacag gcactcatcc
     3421 cacgacttcc ttgacattga cagcactaga aacagtcttg cgtggtggtg aaacattgct
     3481 agatgtggga actgggtcgg gcgtgctaag tattgcagca aaatatcttg gtgcaaaaga
     3541 tgtctatgcc tttgatttag acgaagtagc cgttcgttcg gcgaaagaaa atatggatat
     3601 gaatgaaata gccaaagatg tccatgtatc agctaacgat cttttgacag gaatcgagat
     3661 tgaaagtgat gtaatcgttg cgaacatttt agcagatatc atcttattga tgatcccaga
     3721 tgcttggcgt ttactgaagc aaacaggcac gctgatcgtt tcaggaatca ttgaagagaa
     3781 gaagcaaatg gttctggatg ccatgttaga acaagggttt atcgtggatc aaattttcca
     3841 gcaaaaagac tggtatgcaa ttattttgaa aaaaccagag gtggaataga tgcagcggta
     3901 ttttttaaaa gaatcatatg aatcaaaaga ctattttgtt ttagaagggg aaaactatca
     3961 ccacattgta cgggttatga ggatggaacc cgaggatgaa gtttttttgg cattcaatga
     4021 tcaaatagcc attgttgcag aaatcacgga gattactgaa gaggctgttt atctgaaaga
     4081 aattaaaaaa gaagaaaatc aaaaggaact gccaagttcg attaccatag cgagtggcta
     4141 tcctaaaggg gaaaagctgg attggatcgt tcagaaatcc actgaattag gagcgcaagc
     4201 ttttcttggt ttcccggcat cagcctctgt cgttaaatgg gatgcaaaaa aacgtatgaa
     4261 acgacaacaa cggcttgaaa agattgctca agaggcagcg gaacaatccc atcggcagat
     4321 cgtgccatca gtagagttat tggaaacgaa acaagattta tttgagcggc tagatgatta
     4381 tgacgtggtc ttggttgctt atgaggaatc agcaaagcaa ggcgaaaaat caaatgcagc
     4441 caaaattctt agtgaaacaa aaccagatag tcgaatctta gctgtctttg gaccagaagg
     4501 tggattatcc cgcgatgaag tcgattattt tgtagaacac aaagggatac tttgtggatt
     4561 aggaccaaga atccttcgta cagaaacagc acctctttat ttattaagtg ctgctagcta
     4621 tcaatgggaa ttaatgaatc cttctttcag ctaacgttct tcattaataa aagagtatcg
     4681 acagaaagaa ttgaaaaaat agtcaacaat ttgtataata gaaacaatta tttatacaat
     4741 ggatacgaaa aatcgtttga aaaagctttt aaaaagcgaa catttccgta aaaaaacgta
     4801 taactgcacg gattttggga caattatcaa acagatattt gtcccgattc ccgttttacc
     4861 gaaaaaagaa agggggatcg attcctgtga caaaagaaga aattttaacg ggacccggtg
     4921 tcataaagct cgttagtcaa tacatgggtc ccgaacatgt agcatttgtt gagaaagcat
     4981 gcgaatacgc gactgctgca catgacggac agtttagaaa atcaggcgaa ccctacatta
     5041 ttcatcctat ccaagtcgca ggtatattag cagatttaaa aatggatccc catacagtgg
     5101 ctacaggctt cttacatgat gttgttgaag atacagaaat cactttagaa gatctgagag
     5161 aagaatttgg cgatgacgtt gctatgttag tggacggcgt aaccaaatta gggaaaataa
     5221 aatataaatc ccacgaagag cagctagcag aaaaccaccg aaagatgctg ctagcaatgg
     5281 ctcaagattt acgagttatc atggtcaaat tagctgatag actacataac atgcgtacgt
     5341 tgaagcacct gcgagaagat aagcaaagaa ggatcgctca ggaaacttta gaaatctatg
     5401 cgcctcttgc acatcgtcta gggatcagcc ggatcaaatg ggaattagaa gatacagcac
     5461 ttcgttatct aaatccaaaa cagtattacc gtatcgtcca cttgatgcag acgaagagag
     5521 aagaacgtga aaaatacgta agtggtactg ttgaagatat tcgaatagcg acagaagagc
     5581 tggggatttt tgcagaaatc tatggacggc caaaacacat ttattcaatc tatcgtaaaa
     5641 tgaaagatca gaaaaagcag ttcaacgaaa tttatgactt gctagctatt cgagtgatcg
     5701 tagattcaat caaggattgt tatgctgtat taggagcaat ccacacaaaa tggaaaccaa
     5761 tgcccggcag atttaaggat tatattgcca tgccaaaagc taatatgtac caatctttgc
     5821 atactaccgt gatcggtccg gcaggaaatc cggtagaaat tcaaatccga acacaggaaa
     5881 tgcatgaaat cgctgaattc ggggttgctg cacactgggc ctataaagaa ggaaaaaatg
     5941 aaaaagtaga accagatggt atgacgaaac aattaagctg gttccatgag atactcgaac
     6001 ttcaagacga aagctatgat gcttctgaat ttatggaagg cgtaaaagga gatatcttta
     6061 gtgataaagt ctacgtcttc acaccaaaag gagacgttac tgagttacca aaaggatccg
     6121 gaccattgga ctttacatac agtatccata cagatatcgg taacaaaacc actggtgcaa
     6181 aagtaaatgg caaaatggtg cagcttgatt acaaattgaa aaacggagat atcattgaga
     6241 tcatgacttc tccaaattca tttggcccaa gtcgcgactg gttgaaatta gttgctacta
     6301 gcaaagcaag aaataagatc aaacgtttct tcaaagccca agatcgagaa gaaaatgtga
     6361 tcaaaggcca cgaatccgtg gtcaaatgta ttacagatct aggatttacg cctaaagata
     6421 ttttgacgaa gaacaaactg caagaagcac tcgatcgttt taattatcaa acagaagatg
     6481 atctctatgc agctgtaggg tatggagaag ttagcccctt gacgatggcc aatcgtctga
     6541 ctgaaaaaga acgtaaagaa caaaaaatcg agcagcaaaa gcaagaagca gaagaaatca
     6601 tgaatcagcc gaaaaaagaa cctgacaaaa tgaaagtacg tcatgaaggt ggcgttgtca
     6661 ttcaaggggt agaaaactta ctaattcgta tcagtcgctg ctgtaatccg attcctggtg
     6721 atgatatcgt tggttatatc actaaaggca gagggatatc cattcatcgt cgagattgtc
     6781 cgaatgttca gcctgacaaa ccaaatgtag cagaacgttt gattgaagtc gaatgggaag
     6841 atacatcgaa tacacgaaaa gagtatgatg cagatttgga aatttacggc tataatcgtt
     6901 caggcttatt gaatgatgta cttcaaacag tcaatgcgct aacgaaaaat ctcaacagcg
     6961 ttgaagcacg gacgaataaa gataaaatgg cgacgatcca tttgacggtt ggtatccaga
     7021 atttatccca cctaaagagt atcgtggata aaatcaaagc agtacctgat gtctacagtg
     7081 tacgccggac gaatggatag gaggacagat atgagagcag ttgttcaacg ggtaagtaaa
     7141 gctgctgtgt cagtcgatca gcaagaaatt ggaaaaattg gctacggcct acttgtttta
     7201 ttaggcattc atgaaaccga tacgcaaaaa gatgtggact atctggtaaa aaaaattgca
     7261 caaatgcgta tttttgaaga tgaacaagga aaaatgaata ttagtgtaga agatgtggga
     7321 ggacaaattt taagtatctc tcaattcaca ctgtttgcgg atacgaaaaa aggaaatcga
     7381 ccaagctttg tggaagccgc acgaccagaa acagctattc ctttatacga atcattcaat
     7441 gaaggaatca gaagtcgcgg catcacagtc gctaccggac aattcggagc agatatgtcg
     7501 gtttctctcg tcaatgatgg acctgtaacg atcattattg attctaaaaa caaataaagt
     7561 aatctggttt tcactagtaa aaactatgaa acacgtcgct taagttagac tgataaactc
     7621 taacttaagt ggcttttttg tctgcaaaaa gcgcttcttt tgtcagcagt cggaaaaaaa
     7681 gttaagctta aattatacaa gttatttttt ataggaggtt gctgaagatg aaagaagtac
     7741 cagtagtaga tatcaaaatc acagatttaa aaaaattgta tcaaaaagga tacaacatgt
     7801 tggaggagtt acgccatgaa gcggacgctc ccgtcgtcaa agcaaagatt ttcaataaag
     7861 aagctatcac gatctacgga tcatcagcag caaaagtatt ttatgatcca aggaatttca
     7921 aacgaaaagg tgctatgcca aaattagtat tgaagacact ttttggtcaa ggtggagtac
     7981 agactttgga tggagctgct catcaccatc ggaaaaatat ttttatggat ctaatgacac
     8041 cggaaagaat ggaagattat catcgtatct tagataagaa tctgacacaa gcacttgaag
     8101 cacagcatgg tcaatttgaa ctatttgatt taagtaagat ggttttcttt acatctattt
     8161 gcgagtgggc aggtatcaat ttatcagcga tttctaaaga tgaggtcgaa aaattagcag
     8221 aatatcaaat ctctatgatc agcggaacgt ttacttctcc gattgatcac ataaaaggtg
     8281 tcgagaatcg caaaaaatca gaaaaatggg cgcaaggctt gattgaagaa gctcgacaaa
     8341 atcctgtggc tggaaaagaa aatgtggcat tatatgcttt tgccaatgcc acagatttag
     8401 atggtcagct tttacctctg gaagtggcag ctgttgaatt attgaacatc atccgtccaa
     8461 cggttgctct aactgtgtgg gcggcattga tgggacatgc acttttcagt agaccagatt
     8521 tgtatcagca attaaaaaat gacttttcga cgttacaaga tccgtttatt caagaaatga
     8581 gacgctatta tcctttcttc ccaatgctac ctgcgatttc gctgaaagaa gtagaagtag
     8641 atggttatcg tataccagaa ggcagttggg ttattttaga tttatatgga acagaccatg
     8701 atgaacgaac agttgaagcg ccagattcct ttatgatcaa acggtatgta gggaaagcaa
     8761 aagatatttc ctataaagaa gaatatgaga tgattgcaca aggcggggga aattttcgac
     8821 aaatgcatcg ctgtgcagga gaatggatca ctcttcatag tctacgtgtg ttttccgatc
     8881 agctagtaaa taagtttgag ttctctgtac ctgaacaaga ttggacgatt ccattcaatc
     8941 agtttccgac ttatccgaat agccgagcgc tactttacaa gaattagatt agcataaact
     9001 tatcgaaaaa aaggaagatc aaacagtgct gatcttcctt ttttcatatt actataaatt
     9061 gataaggtca attgatcaaa atccacgaaa tgcttcatca atggtttgat aatcttcatt
     9121 ggataaatgc aaatctaaag cttggacatt gtcagatact tgctctggac gcttagctcc
     9181 aggtataact acagacactc gtggattctt catgtaccaa gctaaaacaa gttgcgcagg
     9241 agtggcttgg tatcttttag caatggggcg aagtacatcg acagcggtta aaatttctct
     9301 gaaacgttct ccttggaaat ctggatttct ttttcgagga tctccttcac caaattgttt
     9361 ttcctcacca agttcatatt ttcctgtcag caaaccggaa gcaagaggaa aatagggaac
     9421 aaaactgatt ttgttttttt caagataagg gaataattct ttttcagcct gccgatggat
     9481 caaactgtat ttatcttcta ctacatcgac atatccatct gcattcgctt ctttcaattg
     9541 ttctaacgtg aagtttgaca caccgactgc tcggattttt cctgcttctt ttaattcgtg
     9601 caatgtagca actgattcat tttttggtgt gctttcatcc ggaaagtgga tataaaaaat
     9661 gtcgatgtaa tcagtttgca gacgttttag tgcatcttcc accgattgtt tgagaaactc
     9721 aggagaatta tcaaatgttc gccctttatt tggtacatga gcagcttttg tagcgatgac
     9781 gacacgtgag cgatcatatt ctttttcctt cagtacctcg ccaatcagtt cttcggaacg
     9841 gccgtttccg taagcataag cggtatcgat caaattgatc ccgctattca aagctgtacg
     9901 aactacttgt tttcctgtct catcttccaa accgtcaaaa agattatgtc ctccaacggc
     9961 atttgctcct aatccaagct gttctgcgta gacttgtgaa tgtccgattc ttatttctgc
    10021 catagctgat tcctcctttt tgtctagtct aaaacaaaat accaaaaagc tgaactaaaa
    10081 cgtttttgtg aggggaaata gtcagatatc tcttgacttt gagactggtt tttagtaggt
    10141 tataagtaat atcgaatgtc aatgaaagag cgagtaagtt tttgaaactt tacaagagag
    10201 gatcatcgag gctgaaaatg atctaaagtt tcattagcct aaagacactc tggagatgga
    10261 cgagtgaaaa gccgtcccgc gtacgagcgt tattcgtaag aggaaagaga aatctttcaa
    10321 aattaggtgg taccgtgtat gaatttcatt cacccttgtt catgatgaac aagggtgatt
    10381 tttttattta catagctaag aaagggagag aaaactatga gtttccaaag accaaaagga
    10441 acaaatgata ttttgccagg cgaatcagaa aaatggcagt ttgtcgaaga aacagcacgt
    10501 ttattattca gagattatca gtataatgaa atgcgtacac cgatttttga acactatgaa
    10561 gtgatatccc gaagtgtagg ggatacaacg gatatcgttt caaaagagat gtatgatttt
    10621 tatgacaaag gggaacgcca tgtgacgctt cgaccagaag gtacagcacc aatcgttcgt
    10681 tcttttgttg agcataaatt gttcggccca gagtatgcga aaccgttcaa aactttttat
    10741 atgggaccaa tgtttcgcta cgaacgccct caaaaaggac ggttacgtca attccatcaa
    10801 atcggagtag aagcatttgg atcagaaaac cctgcaactg atgtggagag catggtgatg
    10861 gcacttgatt tcttcaaaca gttggggatc acgcaaatcc ggctagtcat caattcatta
    10921 ggtgataaag aaacgcgtca agcctatcgt caagcattga tcgactatct tttacctttt
    10981 gaatcagagc tgagtgaaga ctctaaacgc cgccttcatg aaaatccatt acgcgtattg
    11041 gacagtaaag acaaacgaga tcaaaaattt gtggcagaag ctccatctat cttagattat
    11101 ctaagcgagc ctgctcaaaa acattttgat accgttgttt ccatgctgga tgcattggct
    11161 gttccttatg aaatcgacag taatatggtt cgcggactag attattacac acatacgatt
    11221 tttgaaatca tgagtgacgc accaaaaatg ggggcacaag caacgatttg cgcaggcgga
    11281 cgttatgaca atctagtgga agaactaggt ggtccttcaa cacctggctt cggatttgca
    11341 atgggtatcg agcgattact cttgactatg gaggcagagg aagtagttat ccctgcattt
    11401 aatgagttgg atgcttacgt ggtcgcactc ggagacgaaa caaatatcga agcattgaaa
    11461 gtggtccaag cgattcgaaa ctttggtttt tcagctgacc gcgacttcat gaaccgtaaa
    11521 gcaaaagcgc aatttaagac agcagataaa gcgaatgcca aattagtatt ggtgatcggt
    11581 gaagatgagc ttgctagtca aacgattaat gtcaaagcaa tggcaagccg aaaagaaaaa
    11641 agttttccat tagaagagat atatgaacat tttgatgaag tctatgatga aatgacttta
    11701 ttcgagtagg aggaaaaaaa taaatggcac aaagaacgat ctattgcgga ttagtttccg
    11761 aacaacaatt agaacaaaca gttacattaa aaggatgggt acaaaaaaga cgagaccttg
    11821 ggggagtaat ctttatcgac ctacgtgatc gtgaaggaat cgttcaaatc gtctttaatc
    11881 ctgaaatcaa caaagaagca tgggaaatcg cggataaatg tcgcagtgaa tacgtgatcg
    11941 aagtgactgg ggaagttaga agacgcggag aaaatgcagt aaatcctaaa atggccactg
    12001 gtgaattcga agtgattgca agtgaaatcg tgattttgaa tcaagcaaaa accccgccat
    12061 ttcaaattga agatgatcaa gcaatcagtg acgaaatacg tttgaaatat cgttatttgg
    12121 atctgcgtcg tccaaaaatg actaaaaaca tcaaattaag aaatgcgaca acaaaagcga
    12181 ttcgtcatta tttagatgat aatgactttc tcgatatcga aacaccttac ctagggaaat
    12241 ctacaccaga aggcgcaaga gactacctag ttccttcacg tgtccatgct ggacatttct
    12301 atgcgttgcc gcaatctcct caattgttta aacaattgct gatgaatgct ggattcgacc
    12361 gttattatca aatcgttcgt tgtttccgcg atgaagattt acgtggagac cgtcagcctg
    12421 aatttacgca agtagatatt gaaacaacgt tcttaacagc agaagaaatc caaacttaca
    12481 cagagggatt gattgcgaag gtaatgaaag aagttcgcgg tatcgaagtc acattgccat
    12541 ttcctcgtat gacctatgat gaagcaatgg ctcgctacgg cagcgacaaa ccggatacac
    12601 gttttgatat ggaattgatc gatctaagcg atacagtcaa agaagtagaa ttcaaagtct
    12661 tccaaatggc tcttgaaaac ggcggtgtcg tgaaagcatt gaatgctaaa ggcgctgcag
    12721 atcgctactc acgaaaagac atggatcagt taggtcaata tgtaggacaa ttcggagcta
    12781 aggggcttgc atggctgaaa gtagaagaag acggattgaa aggtccaatt gctaaattca
    12841 tgggagaagc aacagaagca atcattaaag caacagatgc aaaaccaggt gacttgctga
    12901 tgtttggcgc agataaaagt gaaatcgtcg ctgctgcatt aggtgctatc cggacacgtt
    12961 taggaaaaga attaggacta attgatgaat cgaaattcaa tttcttatgg gtaactgatt
    13021 ggccacaatt tgaattttca gaagaagaag gtcgatatgt ctctgctcac catccattca
    13081 cgatgccaaa agaagaagat atcccgttat tggctgaaga ccctgcaaaa gtctatgcag
    13141 aagcttatga tatcgtcttg aacggttatg aattaggcgg aggttctatc cgtattcata
    13201 ctcgtgagct tcaagaaaaa gtactagaaa ctctaggctt tacaaaagaa caaatggaag
    13261 aacaatttgg tttcttgtta acagctttag attacggatt ccctccacat ggcggtattg
    13321 cattaggatt ggaccgtttt gcaatgttat tagccggaga agataatatt cgtgaagtca
    13381 ttgcctttcc taaaaacgga aaagcagcag atgtgatgag tgatgcacca agtatggttt
    13441 cgcctttaca attgttcgaa ttgaatattg atgtaacgaa tatagaagaa tagccgataa
    13501 aaaggtaaaa aggatttgtc gaatacgctt cttttgacaa ccttgattta attgaaaaac
    13561 actttataat gaagccataa catataaagc ggggcttttc catgaaaaaa acattcgaaa
    13621 attgggctta tcatgattac acgaccaagc tttcggtatc tatcgtttat gcgttgatcg
    13681 catcagtcgc attgaatttc ttttatcagc caggacatat ttattcaagt ggtattactg
    13741 gtctagctca gattttgacg actttgtcaa aatcactgat aggattcgaa gtaccggtat
    13801 caacaacttt gtatttactg aatatacctt tgtttgtatt ggcgtggtta aaaatcggga
    13861 agaagtttac aattttcact tttatcacag tcactttgac ctccctttgt atccatattt
    13921 taccggaaac agtcctatct accgatccaa tcatctgtgc gttgtttggg ggggctgtga
    13981 tgggactagg tattgggcta gcgttgagaa acggcctatc atcgggcggt ctagatatcg
    14041 tgagtatcac gattcgtaaa aaaacaggaa agtcgattgg ttctatttcg atctacttca
    14101 atgcattgat cgtttttgtt gccggaattc tatttggctg gcaatatatg ttttacagta
    14161 cgttgtctat tttcgtcagt ggaaaagtaa ctgatgtcgt ttatactaaa caaaagaaga
    14221 tgcaggtaat gatcgtaacg aaacatccag aaaaagtagt aaatgaaatc caaaactgta
    14281 tgcgtagagg gatcacgatc atcaatgaag cagaaggtgc ttataagcat gataagcaga
    14341 cagtattgat gacgatcgtt acacgatacg aactgcctct acttagagaa gcaatgaaag
    14401 cttctgatcc acaagctttt gtaagtattg cagaaaacgt ccagatactt ggtcgtttct
    14461 atgaggatgg catttagatc gagtttgaca aaaattcatg aaatactgct gtagacagac
    14521 gttcgtttgt ctgcagtttt tttctaccat agtagaatca gcaaagaaaa actgcttttt
    14581 tatttgaaat tttcttaaag ttgaaaaaaa gcatgtttat tttt
//
LOCUS       DANKHL010000059        14532 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000059 DANKHL010000000
VERSION     DANKHL010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.062"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(107..322)
                     /locus_tag="PW101_001269"
     CDS             complement(107..322)
                     /locus_tag="PW101_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HDL2513676.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(341..577)
                     /locus_tag="PW101_001270"
     CDS             complement(341..577)
                     /locus_tag="PW101_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HDL2513677.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            835..2988
                     /locus_tag="PW101_001271"
     CDS             835..2988
                     /locus_tag="PW101_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HDL2513678.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2957..4372
                     /locus_tag="PW101_001272"
     CDS             2957..4372
                     /locus_tag="PW101_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDL2513679.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            4405..5439
                     /gene="sppA"
                     /locus_tag="PW101_001273"
     CDS             4405..5439
                     /gene="sppA"
                     /locus_tag="PW101_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL2513680.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            5441..5962
                     /locus_tag="PW101_001274"
     CDS             5441..5962
                     /locus_tag="PW101_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL2513681.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            5959..6345
                     /locus_tag="PW101_001275"
     CDS             5959..6345
                     /locus_tag="PW101_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDL2513682.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(6841..6913)
                     /locus_tag="PW101_001276"
     tRNA            complement(6841..6913)
                     /locus_tag="PW101_001276"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6878..6880),aa:Lys,seq:ctt)
     gene            7294..7863
                     /locus_tag="PW101_001277"
     CDS             7294..7863
                     /locus_tag="PW101_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDL2513683.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            7850..8710
                     /locus_tag="PW101_001278"
     CDS             7850..8710
                     /locus_tag="PW101_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2513684.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            8900..9604
                     /locus_tag="PW101_001279"
     CDS             8900..9604
                     /locus_tag="PW101_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2513685.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            9609..11444
                     /gene="walK"
                     /locus_tag="PW101_001280"
     CDS             9609..11444
                     /gene="walK"
                     /locus_tag="PW101_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDL2513686.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            11441..12757
                     /locus_tag="PW101_001281"
     CDS             11441..12757
                     /locus_tag="PW101_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513687.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            12758..13627
                     /locus_tag="PW101_001282"
     CDS             12758..13627
                     /locus_tag="PW101_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDL2513688.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            13682..14491
                     /locus_tag="PW101_001283"
     CDS             13682..14491
                     /locus_tag="PW101_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2513689.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
BASE COUNT         5024 a         2312 c         2922 g         4274 t
ORIGIN      
        1 ctttagtaaa aagttctgtc cattccaaac ttttgaaaag ataaagaaca tacctatgtt
       61 cgtaatcgac acaaaccaat cttgaggttt gatatttttt aattgtttac gcaccggtcc
      121 acatgctgaa tccagcacct aataaagctc cacctacacc acaagcagct attgcataag
      181 gtcctccggc tgaacaaagt tttactcctg ctgttgcccc agaaaaagta gtgcctaaaa
      241 ctcccacggc actcccacct tgaatatttt tcaactcttc tgaagataat ataaatgttg
      301 tttttgtgtt tttaatatgc atactactta ttctcctttt ttattttttt cgagcatccc
      361 agccagctgc agcacctcca gcgacagctc catataaccc tccaacaaca ccgcaagctg
      421 gtccgcaaat tgctgttcct aaacctgcac cacctactgc ccctgtggta agtccaccta
      481 caacttgccc caattttccc caagcattat tgcctccacc aacagtttgt gcatttccac
      541 aatacttagt tcctttacat tttgcatgtt taaacacgct tctttaaata atatttttta
      601 cggtgctact gtaacatacg tatttgatta acagtaaaaa tcttcctgaa acttatcaag
      661 tttattcttg aatagtaaaa tttccaaaat aaatttgatt caggaatgaa aaagttagtg
      721 ttcaagaact gttttcaaag tttttgtgga attatgagac aatattttca ctgtattaaa
      781 gctatattga gaatttttct ataaaaattt tttagaagga gttgtgttag ataagtgata
      841 aaatcatttc cttttgtgca gcaacaagat gaaaaagatt gtggtgttgc ttgcctttcg
      901 atgattttaa aatattttaa aactgatatc ccgattcata aattacggga cctatctggg
      961 actgatttag agggtacttc agcatttgga ttgaaaaaga catttgaaaa attgtctttt
     1021 gattgtatgg ctattcaagc agatagtgag atctggaaag aaagagatct tatcttacca
     1081 ttaattgcac atatattggt tgataacagc tatatgcact acgttgtaat ttataaaacg
     1141 aagggaaacg tactatggat tgcagatccc gctaatggaa aaaatagaaa aacgattgaa
     1201 gcgtttcaaa aagaatggac aggtattctt ttattaccta tgcctagaga aacttatatt
     1261 cctattaaag aaaaagtagc tggattaagt tcttttttgc ctataatttg gaaacaaaaa
     1321 ggattagtat tccacatcgt tttagcatcg attttcatta cgttttttgg cataggaagc
     1381 tcatattatt ttcaaggtat tcttgatttt ttcatcccta atcaagcacg ttcaacttta
     1441 aatataattt ccattggctt gatcgctgtc tatttatttc gggtattgtt tgaatatagt
     1501 cgtagttatc ttcttattat tttaggacag cgaatgagta tggcaattat gcttcagtat
     1561 ttcaaacatg tgttaacttt accaatgaat ttttttgcta cgagaaaatc aggggaaatt
     1621 atttctcgtt ttcttgatgc cagtaagatt attgatgctt tagcaagtgc tacgttatca
     1681 atatttttag atattggtat ggtgttgctt gttggggtta cgttagctat tcagaatagt
     1741 acgttgtttt taatcactct atgctctttg cctttttatg tagttgcaat attcgctttc
     1801 gtgaaaagct atgaaaaatc gaacagggaa gaaatggcag ccggtgcagc tgtaaattct
     1861 agtatcatag aaagtcttaa aggaatagaa acaattaaag catatagcgg tgaagagaaa
     1921 gtatataacc gtatagacca tgaatttgtt aaattaatga aaaaatcatt tcgtacggtt
     1981 acgcttgata atattcaaca aggaattaaa catttgattc aattaattag cagtgcatta
     2041 attttatggc taggttctta ttttgttatg gataatagaa ttagtctagg tcaacttatc
     2101 acatacaatg ctttactagt atttttgaca gatcctttac aaaacattat taatttacaa
     2161 gtgaaaatgc aaacggctca agttgcaaat aatcgtttaa atgaaatttt cgcgattgat
     2221 tcagaaaaat cgaaccagtc ttttgaatat atgctttctg aacaattatt tcaacaagat
     2281 atcactgtaa aaaacgtttc attctcgtat agcatgaagg ccccaacgtt gaacgaaatt
     2341 tcatgtacaa ttccttcgca cagtaaaacg gctatagttg gagttagtgg ttctggaaaa
     2401 tcaactcttg caaagttatt tgttcgtttt tatgaagcat cagaaggatc gatccattat
     2461 ggaaacatta atcacttaga cattcctttt cagatattac gcgatcgagt gacatatttg
     2521 cctcaagagt catttttctt tagtggaaca atcattgaga atcttatatt cggtttagat
     2581 agcccaccaa gtttcgaaca aataatagaa gtttgtgaga tagtccaact aaatgaattt
     2641 attaatcaac agccattacg ttacgagaca ttgttggaag aaggtggaaa taatctttca
     2701 ggagggcaac gacaacgatt agccttagca cgggctttat taaaaaatcc ggacatatta
     2761 attttagatg aagcaactag tggattagat accttgttag aacatgcaat tatgaattat
     2821 ctcctcaatt taaaagaaaa aacgattatt tttattgcgc atcatttacc aatagcaaaa
     2881 gcatgtgatc aaattttagt tctccatgaa gggcatttgg tagaaaaagg aacgcacgat
     2941 gaacttcgtt ttgccgatgg tatctatcaa aaattatggg aaatctagga gagggaaaca
     3001 atttatgcag aataataatt ggttcgaaca ttctagtatc tatactaaac aacatcatag
     3061 cttctaccgt tgggtgttat accctgtaat tcttttttta tgtattattg gattgttttt
     3121 aacctttgct aaaaaagagg ttgttattcg gacaacggct caattgaacc ccgaaaaaat
     3181 agagaagtta caagtgccat tagaagctaa aataatggaa aacttcctaa aagaaaatca
     3241 atttgttcat aaggacgatg tgttggttag attggattgt tctttaatag agaatgaaaa
     3301 ggctcagata gaacaagaaa accaacgtat tactcaacaa ataaagatgg ctcagctatt
     3361 tattgaaagt ataagtaaag gaaaaaattt gttttcaacg gatgacagtt ttggctacag
     3421 taatcaatta aagagcatgt tgtcagaaaa agaatcactc cgctacgctt tgaagcaaag
     3481 tgaattaaat gatcaaaagc aattagaagt atacgaaaag acaaaaagac aactagaaaa
     3541 acaaattgag agttcagata gtaaattaca agaatggcaa caagtacagg tagcttggag
     3601 taataatcaa tcattaaaag atttttcaaa agaaatgatg gcaaactatg agaattggca
     3661 agaacaacta aataatgttt ctgatgatca aaaaaatcaa gtgaaactga caatttcagc
     3721 aagcataaat gaacaaattg agcaactaaa aaaagaagta gaacagtatc agtcagaaaa
     3781 agctaaatta gttaaaccaa ctacttctga gaatgacaga attagtcaaa cggaaaaagg
     3841 aaagcaagag ctagaacaag caataacaac tgtaaaacaa acagttacag agctaaatga
     3901 aaaacaagag aagaaccagt cagttatcaa aagtttggac gatcaacttt caaaaggaat
     3961 actaaaagct cctgtatctg gaacggttca tttaaatgaa gaaacaaagg gacaagcaga
     4021 actagccaaa ggaacagtgc ttgcagaaat ttatccgatc cacgaaaaat caaaaatgaa
     4081 atttacagca cttttacctg cgaatgaaag tacctatatt aaagaaggga tgaaggttca
     4141 ttttaaattg gatcaaaaag gtggggctcc gattacgatc gacggaactt tagatgaaat
     4201 ttcagaaaat tctactgcca ctgagaaagg tgttttttat gcagtgaaag gaacgttaaa
     4261 aacaactcaa tcgtttccgt ttcgttatgg attaactgga gagttgtctt taattgtagg
     4321 agaaaaaaca tattggcaac aaattgtcga tgtgtttcta aataaaaaat aattattttt
     4381 actaagaata taggaggagt ttatatgaat aaaagaagat ggattgctgt gggagtagcg
     4441 tgcggattat tgttactatc cattatcgtc gcgctaattc caggaaaaga taaagaggaa
     4501 gcatcaaata caaccctaac aggaataaat aagatttttt acggatctaa cgaaatcaca
     4561 gaagaaacgt tagaagaagg cgcatcaaat aaaaagattg tcaaactatc cgtcaatgga
     4621 gttattgcag atacaggtga aagcaatctt tttagtaggg aacaatataa ccatcagaat
     4681 tttttaacac agctaaagaa aattcaagaa gacaaagcgg ttaagggagt gttattagaa
     4741 gtaaattcac ctgggggagg aatatatgaa agtgctgaga ttgctaaaga aatggcaaaa
     4801 attaaaaagc tagatattcc tatctatact gcatttaaaa atacagcggc aagtggaggg
     4861 tactacattt ctgctggttc agataaaata tttgctacgg aagaaacaac aactggatca
     4921 attggtgtca ttatttctgg tttaaattat tctggcttat tagaaaaact tggcgtaact
     4981 gatgcgacgt ataaaagtgg tgcattaaaa gatatgatgt cacctcaaca caaaccttca
     5041 gaggaagaga ataaagttat acaggaattt gtgatgagtg cttatgatcg ttttgtcaat
     5101 gttgtagcaa aaggaagaaa tatggatact aatgcagtga aagaacttgc ggatggaaga
     5161 atttatgatg gtaaccaagc agtagaaaat gggttggtgg atcaaattgg ttactctgaa
     5221 gatgcattag attccctaaa aaaagagaag aaattaactg atgcgacggt tatagaatat
     5281 aaaaatgaca caactggctt tgcttcttct tggttaggta ataagattgc tgaatggcaa
     5341 gggctaaagg caactacatc agatcgtgta atttcagttt ttgaaaaact tggattttct
     5401 gatactccaa aaccaatgta ttactatgga gggctataaa atgaataaga agagtggcat
     5461 tgaaactttg cctaattatg tatttgcggg attttggtta cgattttttg cttttcttat
     5521 tgatttaatt tgtattagca tcatcaccca tgctactata ggtctctttt acaaaatcat
     5581 gggactggaa tctgacaaat ccattattac tttttatggt atagcgtcat tagctattta
     5641 tttaacctat tttgtacttt tgactaagct gaattcagga caaacaattg ggaaaatgat
     5701 ttttgggata agagtagtga gctttgagga aaaagagcta agttggcaaa ctatctttat
     5761 tcgtgagtgt atatgtaggt ttatattaaa aaattttgta tttcaaattg ggtatttggt
     5821 ggctgctttc acgcccaaga aacaacatat tggggattat ttttctaaca cgtcagtggt
     5881 tgttattcgt tcattagaag ctattaacta tggtcaaaaa atttctaatt ctaaaacact
     5941 taatctggag agaattaaat gaataagaga aaaacagagt ttattttgat tttaattagt
     6001 ggaataacag ggattttatg tagtctttta gcattgatgg gtggtatgtt tctatcttct
     6061 attgattcaa ctactttaaa tactacagaa ctaaccagta aggatataga tatgttagtc
     6121 aatctaagcg ggaatgttgt tttatttgga ggaattaccc tagttttttc gataatgatt
     6181 ttcataatag gttttttagt aagaaaaaat caaaaaacag tggtctatgg tattagttta
     6241 ctcattttat ctattattcc attttttctt ttaacattat tgtgggtaat tcctggagtt
     6301 ttaggtatta ttgcgggatt gatgcttcta ataagaaaat cttaattagg caatgtgtag
     6361 acaacgtgaa acgattaatc aattaaaagc taaatacgtt ttgagaaatg ataacttgta
     6421 gtcgatggaa gacatgtaga taaaaaaact acgtgtcttt tatttattct aattttttcg
     6481 aaaaaaagac actctctgat tgttgtatta atggaaaagg ggaaagcagc aagtaaattt
     6541 agtttttcta ttaaatattc ttcacttttt aaccttttat agtacttaga tttaatccta
     6601 tatgtttaga gagttcattg gcagcatgat tttgcaaagc ttctcgaatt attgttatat
     6661 tagaaacttt actgttcata attatgcttc ttttttaact caaagtccaa agggctacag
     6721 tggactttga gttaaaaata aaaagcccta gttattttga aaaataaaaa aagaacagcg
     6781 taaatgtttt cgaaatgctt tcgcaccaac atttacctgt tccgtgggtt tggcacccaa
     6841 tgggccgtga ggggctcgaa cccgcgaccc gctgattaag agtcagctgc tctaccaact
     6901 gagctaacgg cccgtacaaa aagtacttaa taatagtagc actaagaatt ttaaaatgca
     6961 agactacatt taaagaaaat cagaaaaatc tttacttcct tataggaaat cattcataga
     7021 ttattcacat ttgctcgcta ttatataagc ataccaacaa acaaagttct tgcaagagaa
     7081 caaaccctaa cacttttttc attttttact cctttcccgc cgtctccccg gcgggtctcc
     7141 tgttcttaac gataaaagac aagaaggctt agccttcttg ttttttgttt tcatggaatc
     7201 ccgctaatca aatcacctag ttaaagcaac tagattgaat gtttatcata agtctgatat
     7261 catgtttatg aaatgaaaaa aggagcggtt atgatggaaa aaatgcgtca gcaattaatg
     7321 gagtggggga aggaactaga aacattccgt ctgcctcact gggaagaact ccctgactta
     7381 gaactttata tggaccaagt cagaacgtta gttgatcgtt atctttcacc tgtgatacaa
     7441 ggagaaaagc atccattgct tacctcttct atggtaaaca attacgttaa actaggtttg
     7501 ataccagcac cagttaaaaa aagatataac aaagaacacg tagcattttt acttgcaatc
     7561 actacgttga aacaagtatt gactattcct gaaatcaaag aagggatatt gttccaagga
     7621 aaaacagtag gaattcgtga agcctataat ctattttgcg atgaacaaga agcagctgtg
     7681 tggatggtca gccaattagc acaaggtaaa tctcatccgc aaaaattcga ccaggcaacc
     7741 cctgtagaat atattgctgt aagagcggca actttatcgt ttgcaatgaa acttcttgcg
     7801 gaaaaaacga ttgttctaga aaccgaatat ttaaaagagg agaaaacaaa tgaaaaacaa
     7861 taaaatcgca attttagtag attcaggtac ggatgttcca gaagaattga taaaaaaata
     7921 cgagatgtat atgatcccat tgaaaattat ttataaagat caggtatata cagataaaat
     7981 tgacattacc cctgaagaga tctatcaacg tttgccagag gaaatccctg gtacatcttt
     8041 gccggatggt gagtcgatca cagaaatctt tgagcaaatc aaaaaagacg gttatgaaaa
     8101 ggtacttgcg gtaacaattt ccagtggatt gagcgggaca tataatgtgg ttcgtttaat
     8161 tgcacaacag caagaagaat tggaagcata tgtactagat acgaaaaata ttggtattgg
     8221 tgcaggtttt tctgcgatcc aagcagcaaa atggctggaa gaaggagtag aatgggatca
     8281 actgatcagt aatctgaatg agttagtcga aagaacaaaa gttttcttta atgttgcgac
     8341 attggaatat cttcaaaaag gtggacggat cggtttagtt gcttctattg taggaaccgc
     8401 tttaaagctt aacccaatca tctcatgtaa tgaagaaggg atttattaca cagtaggaaa
     8461 agctagggga cgtaaaaaaa gtttggataa aacggttgct tacgtcaaag aaaaaatcgg
     8521 taatgcaaag gtcttcaact tagctgttgc ccatggggat gcgaaagaag aagcaattga
     8581 aatggaagcg agacttaaaa gagagttccc acaagcaaac caaatctact ttggccagat
     8641 ttctccggca ttagtagtcc acactggtcc gggattattg ggtgtcggtg tacaagtttt
     8701 agaaggataa atcaaaaaaa ggaaacactg aggtgaaaat cagtgttttc cttttttatt
     8761 gaagttagaa caaaaaataa taaagtattt tgcactttca ttttaatttt ctcttttgtt
     8821 ttataggcaa agatgaaaca aaagcagaaa tacggtataa tgagaaggta tgacaataat
     8881 atataagagg tggctcttta tgaaaaaagt tttagttgtc gatgatgaga agccgatttc
     8941 ggatatcgtc aaattcaatt tagccaaaga aggctatgac gtttatactg catatgatgg
     9001 ggaagaagcc ttggaaaaag ttgctgaggt agaacctgat ttgattttac ttgatttgat
     9061 gctacctaaa atggacggct tagaagttgc tcgtgaggta cgcaaaactt atgatatgcc
     9121 gatcattatg gtaacagcca aagattctga aattgataaa gtcctaggct tagagttagg
     9181 cgccgatgat tacgtgacca agccattttc caaccgagaa ttagttgcac gtgtcaaagc
     9241 aaatcttcgt cgcggtgcca cggctgcgaa ggaaccagaa gaggcagcac ctgcggaatt
     9301 gacgattggt gatttgacga tccatccaga agcttacatg gtgacgaaac gaggagaaac
     9361 aatcgagtta acacatcgtg aattcgaact actcttttac ttggcgaagc accttggaca
     9421 agtgatgaca agagaacatt tattacaaac ggtttggggt tatgattatt ttggagatgt
     9481 acgaacagtg gatgtaactg tacgtcgttt gcgtgaaaag atcgaagata atccaagcca
     9541 tccaaattat ttagtgacac gtcgtggtgt cggttactat ttgcgtaacc cagaacagga
     9601 gtaattggat gaaaagaaaa gtccgctttt ttcgatcagt caattttaag atcgctatta
     9661 cctttattct gattttattg atctcgatcg aaattattgg tgcctacttt atacgtggtt
     9721 tagaacgttc cacgatcaat acatttatta aagatatgaa tcaaacggtg gaatcattag
     9781 caacaacaat cagcccggaa ttgaatcgaa aagataatgc agatgatgaa gaggtaaatg
     9841 cgaatatcaa aagatttatt gaaaacagtg cgactagcga tatcatcgaa atacgggtcg
     9901 ttgacgaaaa agggatcatc cgaggaacaa ccgatgtcaa tgaacaaagc gctgtgggaa
     9961 agaaaaatga ttatgttgat atcaatgatt tcacgactaa acgttatgtt gctttagata
    10021 acgacgataa acgggtgaat atcaatgttc aaccgatcct gtccccgacc ggcgatgccg
    10081 ttatcggggc tctttacgtc aaaagtaata tcgaacaaaa atattcggaa atcaataaca
    10141 cagcagtcat tttctttact gcctcactaa ttgcggcatt tatttcaatg atcgtatctg
    10201 ttttagttgc tcgttcgatt acacagccga tcggtgaaat gagagaacaa gccattcgaa
    10261 tcgctagagg agactacagt cggaaagtca aagtgcatgg acaagatgaa cttggacagc
    10321 tggctgatac cttcaatcag ttagctgaac gtattgaaga agctcaagat acaatggaag
    10381 ctgaacgcaa tcgtttggat agcgtgctat cccatatgac agacggcgtc attgcgacag
    10441 atcgcagggg taaagtcatt acgatcaatg atatggctgt ttccttgcta gatatcaaaa
    10501 acgaggaagc aatcggacaa tcgattttga cacttttaga tatcgaagaa gaatatacgt
    10561 tgcggaaatt attagaagat ccaaacgaaa tggtacttga ccgttcaacc agtacattgg
    10621 aaagtgatca gatgatcttg cgagtagact tttctatgat ccgtcgcgaa tcaggtttta
    10681 tcagcggatt agtagctgtc ttgcatgatg tgacagaaca agaaaaaaca gaacgggaac
    10741 gtcgtgaatt cgtctctaat gtttcccatg aacttcgtac accgttgaca agtatgcgta
    10801 gttatatcga agcattgagc gaaggtgcgt ggaaagacga ggaagtagca ccaaattttt
    10861 tgaaggtaac gctagatgaa acagaccgaa tgattcgaat gatcaatgac ctcttaaatc
    10921 tatctcgcat ggatacaggt aatacccagc ttcaattaga atatgtcaat ttcaacgagt
    10981 tagtgaattt tgttcttgac cggtttgata tgatggtggg aaatcaggaa aaaaattata
    11041 agatccgtcg tgaatttacg caaagagatc tttgggtcga agtagataca gataaaatca
    11101 tccaagtagt cgataatatc atgaataacg cgatcaagta ttcaccagat gggggaacga
    11161 ttacttgtcg cttattagaa acgcataata acgtgatttt aagtatcacc gatcaaggat
    11221 tggggattcc taaaaaagat ctgaaccgag tgttcgagcg gttctatcga gtagataaag
    11281 cacgtgcaag agcacaaggt gggacaggac ttggattagc tatttctaga gaagtgatta
    11341 aggcgcatcg aggcgctatt tgggcagaga gcaaggaagg aaaagggtcg actttttata
    11401 tctcgctgcc atatgaacct tatgaggagg aatggtggga atgagaatct ctgaaaaagt
    11461 cgttcgtata ggactcattt tgatggttgc gttaagcata tacttctctt atgcaatctg
    11521 gctgagccca gcaggaaaaa gttcaatcaa cgtagatgag acaaacactc aaatgatcga
    11581 gtctcaaagc aatcgaaaaa cttccgaagt ctttcttccg cttcatgtta catggcacca
    11641 ttcgggaatg atccaagaaa ccaacagtga aaatctcgtg taccgattgc aattgatcat
    11701 cgataaagca agatttggaa agctttctga agtagtttca ggagacaaat cacagtttga
    11761 acaggtgaaa aatctgaact ctggttttga gttggcctat aatgcgcctt ttttgctaag
    11821 agaatacaag gaagcatttg atttagattt agatgtgtcg aacttggcaa aagaaagcga
    11881 aaatatgtat tttactagaa tacaattcga tcaaggaaat aaaaaaatcc gatttttgaa
    11941 ttattcgaac catctcgtat atgaagcaaa tatttcgttg aattggacag atatcaataa
    12001 agaattaaca gcaaaagaca caaaatggac agaaatgaca acagaaccat cgacgatcga
    12061 cacgcaatat gctacgaaac attcgatcaa actgaagaag tatagttata tcctgtcaca
    12121 gcaaccctat acacttttta gaaatgcttt tttccagaaa ccagatagcg tgaaaaataa
    12181 tgatgaaagc aatgaattga ttttttacgg tgggaatgaa acaatgtcta tccattcgga
    12241 aacgcaagta gttgattttc gtggagagct gccagagaaa aaagaaaatg ctagtgtatt
    12301 ttctgaaagt ttcccctata ttagcaaatt aggcagttct ttagggaacc tcagatattt
    12361 tgaccgcagt gacaatacga tcgattatcg gatattcgtg gaaggcttcc cggtttttgg
    12421 gacagacagc agaggacaga ttggtgtgga agttggcggt gaaacgaatg gttctgtgca
    12481 agtgaatatc caaaccagtt tgaatacgat tcaagtgccg ataccttcag acgaagaagt
    12541 agaacttgca agtacgaatg aaatcatcca gcagttaaaa gctaagggag cagatagcaa
    12601 caagattggt tcagtgatcg ttggctatac atggcaaaat gtcaaagaaa cgaatcgtgt
    12661 cgttgatctc ttacctgaat ggtatgtgaa atataaagat aactggtatt cagctaacga
    12721 ccttctggaa aaattacctg gattggaggc gaattaattg gatttcaaaa aaatcgaatg
    12781 gatatttttc gtcgcttttt tagggttgaa tgtgttttta ttcagcagtt atcatgacgc
    12841 ggtataccaa gaaaacaatg tcattcgctc taatcagact gaatcaatcc aacaacgtct
    12901 ggctagcgac gatatcactt acacggggaa gatttcgtct gagaacaagc aaggctatta
    12961 tcttagcgcg gagcagacca atcttagcac ttcattagcg aattatcgca aggctggtaa
    13021 aagcggcttg ctggcaagtg gtgtgttttt agacggtgat gttttgacga aatctttatc
    13081 cagttcagat gctgaaaaga acgtgatcga tccagataaa attagtagtt ccttggaaga
    13141 ttttttatct aacaaagaca atattttatt cggtaatgat tatgtgtaca cgaaaaactt
    13201 ttctcattta gaagaagaac cactagaaat ccaagcaagc caagagtatg aaggcatccc
    13261 attcaatgat gatacagcaa aaatcgttct ttctttagaa aaaaacgata atgattatag
    13321 catcacgaaa tacatgcaga cgcatttatc tgatatcgag cagctgcgcg aaaagacgga
    13381 actgtatacg gaagaggaag caatcaatac attgtacatc aataacaaaa taccaagagg
    13441 atcaaaaatt ttatggcgtc agctagctta ttcctgtatt ctgaaagtcc gggaaaaaaa
    13501 tgtgtatgtt cctgtttggc atgtagcgat cgaaacaagc gataaagtgg tccaaattga
    13561 gagcgtcaac gctttcagca atacgatcgt gacaaacaat actgtaccaa aagtggaaga
    13621 tcaataaaaa agcgagtata atgaaaaaag tttacaaagt aagaagggaa gtaggagtca
    13681 aatggctgaa gaaaaaccag cttttaaaat cagtatccta gcaagtggaa gttcgggtaa
    13741 ttcactttat atcgaaagtg aaaaaaaacg gcttttggtc gatgcaggac ttagcggtaa
    13801 aaaaatcact tccttgatgg ctcaaattga tcggacaccg gaccaattag atggaatact
    13861 tgtgacgcat gaacatcgag atcatataca tggcgtgggt gtcttggctc gtaagtatca
    13921 tttggatgtt tttgccaatc agcagacttg ggaagcgatg gaaccactga ttggaaatgt
    13981 accagtggaa caaaaacatc tatttgagat ggggaaagtc agaacattcg gcgatatcga
    14041 tattgagagt ttcggcgtct cccatgacgc agcggctccg caattttatc gtttccataa
    14101 agaaaatcgg tcattcgtca tgttgacgga caccggatat tgcagtgatt atatgcgagg
    14161 tgtcattgaa aatgccgatg gttatttgat ggaaagtaat catgatcttg agatgttacg
    14221 catgggacct tatccgtgga atttaaaaca gcgtatttta ggagatagag gacatctttc
    14281 caacgaagac ggtgcacttg tcatgactga tgtgatcggt aatcggacca aacgaatcta
    14341 tcttggccat cttagcaagg aaaacaatat gaaagaactt gctcatttaa caatggaaaa
    14401 tgttttgaaa gaaaaagatt ttggcgttgg acatgatttt gaaatctatg atacagatcc
    14461 agatgaagca actgagctat tttcgattta aacagttcaa tcaataaaaa tataaaaagc
    14521 agaagataaa at
//
LOCUS       DANKHL010000060        14520 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000060 DANKHL010000000
VERSION     DANKHL010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.063"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(130..825)
                     /locus_tag="PW101_001284"
     CDS             complement(130..825)
                     /locus_tag="PW101_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HDL2513690.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(915..2015)
                     /gene="ychF"
                     /locus_tag="PW101_001285"
     CDS             complement(915..2015)
                     /gene="ychF"
                     /locus_tag="PW101_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDL2513691.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2024..2215)
                     /locus_tag="PW101_001286"
     CDS             complement(2024..2215)
                     /locus_tag="PW101_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDL2513692.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2236..3126)
                     /locus_tag="PW101_001287"
     CDS             complement(2236..3126)
                     /locus_tag="PW101_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDL2513693.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3113..3880)
                     /locus_tag="PW101_001288"
     CDS             complement(3113..3880)
                     /locus_tag="PW101_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2513694.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="PW101_001289"
     CDS             complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="PW101_001289"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDL2513695.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="PW101_001290"
     CDS             complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="PW101_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDL2513696.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="PW101_001291"
     CDS             complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="PW101_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDL2513697.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8451..8834
                     /locus_tag="PW101_001292"
     CDS             8451..8834
                     /locus_tag="PW101_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513698.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(9002..9760)
                     /locus_tag="PW101_001293"
     CDS             complement(9002..9760)
                     /locus_tag="PW101_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HDL2513699.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9778..10596)
                     /locus_tag="PW101_001294"
     CDS             complement(9778..10596)
                     /locus_tag="PW101_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL2513700.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="PW101_001295"
     CDS             complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="PW101_001295"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDL2513701.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="PW101_001296"
     CDS             complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="PW101_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDL2513702.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11725..13059
                     /gene="dnaA"
                     /locus_tag="PW101_001297"
     CDS             11725..13059
                     /gene="dnaA"
                     /locus_tag="PW101_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDL2513703.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13257..14387
                     /gene="dnaN"
                     /locus_tag="PW101_001298"
     CDS             13257..14387
                     /gene="dnaN"
                     /locus_tag="PW101_001298"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDL2513704.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         4027 a         3054 c         2595 g         4844 t
ORIGIN      
        1 tttatcgtaa aaagggactg tgacaagact tttgtcacag tcccttacta ttatttaact
       61 atggataaac aaaatgaggt tctttactac ccctagatat ctagcaaaat agctgatctt
      121 tttgctagtt tatcttcttg ggacggataa acttccaata atcccgtatt ttttcttcaa
      181 gtagtaacgg attgctaaag cagttgcccc gataatgata actactactg gatctaatac
      241 tggattgata actggtggca atagtgttgt cgcagaaaac agtacgaccc aaagaagtgt
      301 cgttccacct aggataagca atgtcttacc cattcctggt ttcttgcttc gatctgctcc
      361 gggatattca tattgataga tgtatttgta catcatataa aagacccagc caccgattgc
      421 agaacctaga acgagtgtag aaaagccata agctggcgtt gttccacgtg aaaacaatcc
      481 ggtaatcgca atcatgatac ttaatagacc aagcaacagc aacgtattat ctaaccacat
      541 caataaaggg gtggatgaat tttttacttc tgctggctta ttgatgattg cttccgttct
      601 ttcagagact gtcccaaaca attgacgggc tgttcttccg cctttttgtt ctgcaactaa
      661 agtaggcaga atgtcatgaa gggcttttac tttttcttct tcagacaaat tcgctgcttc
      721 taaagatttt ttcaaatcaa aaatgtactg ttgatttctt ttggttaatt ttgtttctaa
      781 actattgttt tcttttacga ttgctcgtaa ttgttctggt tccattatct aatagtcctc
      841 cttagccgtt ccttttctca acggtcgtca caaaagacat tctaccatat tctacagcta
      901 gtttgatagc ccttttacac attaaagcgg aataacatta cgtcgccgtc ttgtacgaca
      961 taatcttttc cttctaatcg aacacgtcca gcttcttttg ctgcatgcat gtttccgtaa
     1021 tgatccaagt cttcaaaaga gacagtttct gcacggataa atccgcgttc gaaatctgaa
     1081 tggatgattc ctgctgcttg tggtgctttg attccttttt tgaatgtcca agcacgtact
     1141 tcttgttccc cagcagtaaa gtaagtagct aagcctaaca aatcataagc agcacggatc
     1201 aactgatcca aacctgattc ttcgattcct aaagcttcta aaaattccgc tttgtcttct
     1261 tcatctaatt ctgcgatttc ttcttctgca cgagcactga ccacgatcac ttctgcattt
     1321 tcttctgcag caaagttgcg cacttcttgt acataatgat tcgcgtctgc atctgctacg
     1381 tcatcttctg agacattggc tacataaaga attggttttg tcgtcaataa aaatagtgat
     1441 ttaacgattt tttgttcttc ttcagtaaat tcgatcgttc gggcagattt tccttcttcc
     1501 agtacaggtt tgattttatc cagtacagct agttctgcca ctgcatcttt atctttggtt
     1561 ttagcaactt ttgctacacg agtatagcgt ttgttgatag attctaagtc tgctaacact
     1621 agttccaagt tgatcgtatc aatatcttct agtggatcaa cacgtccttc tacgtgagta
     1681 atattatcat catcaaaaca gcgaacaacg tgacaaatag cgtctacttg gcggatatgg
     1741 cttaagaatt gatttcccaa accttctcct ttacttgcgc cttttacgat tccagcaata
     1801 tctgtaaatt caaaagtcgt cggaacagtt tttttcggat gtacaagttc tgttaagcgt
     1861 tgcaaacgcc agtcaggtac ttctaccatc cctacgttgg ggtcgatcgt cgcaaaggga
     1921 tagtttgctg cttctgctcc tgcttttgtg atcgcattaa acaaagttga ttttcctacg
     1981 ttgggcaacc caacaattcc agcagtcaat gccataaatt tattcactct ttctctgcat
     2041 ttttttcaat tacttttttt aatttctttt caaaatctcg acgggtcatc atgacgatat
     2101 gtccgcaatt catacatctg attttgatat ctgcgcccat tcgaatgact tcccagcgat
     2161 tcgtctggca agcgtgaggt tttttcattt caacgatatc gtgcaagtcg tacataaaaa
     2221 tagcctcctt tttgtttatt cttcatcaaa ttgaatattc aagatatcaa gaattcgagt
     2281 caaatctttt tgagaaagat attcaatctc gatcttacct ttcccatttt tttcttggat
     2341 agctacactt gtaccaaatt tatccatcaa acgatcttcg ctttctcgga tataatatgg
     2401 tttttccttt gctagccttg ggattttttt cttttctttt ccatcatttt gatttaactc
     2461 agaaacaagc tgttccagtt ggcggacagt taaattttca gttactgcac gattggctaa
     2521 tttcaagatc aatgttttat tttttaaacc taacagcgta cgggcttgtc ccatagataa
     2581 acgctgatct tgtaccattt ccttgacaag atccggtaaa gaaagcaagc gcaaataatt
     2641 ggcgatataa ggtctgctct ttcctaaacg actagctact tctgcttgtg ttagtttcag
     2701 gtttttcatc agcatctcat atgcttctgc ttcttctaac ggattcagat cttctcgctg
     2761 caagttttcc agcactgcta cttgcatcat cgcttcttcg tcaaagtctc taacgatagc
     2821 agggatcgtt tctttttcag ctagtttgga cgctcgaaaa cgtcgctccc ctgcaataat
     2881 ctcatatcct tttactgcgg aacggcgtac gatgatgggt tgaaagacac ctgattgttc
     2941 aatagaatta gctagttcct gcaaagatac ttcttcaaat gttttacggg gctgatacgg
     3001 atttggtcgc agctcattca atggtagctg gacaacggta tcatctttta catccaccgt
     3061 ctctaagttt tctaaatctt gaaacagtgc atcgattcct cggcctaatc ctttactttt
     3121 cttcacgact taccacttcc tttgccaatg cttggtagac ttcagctcct cgagaacgtg
     3181 gatcgtagtc gatgatcggt tttccatgac taggtgcttc tgataaacga atatttcttg
     3241 gaatgatcgt ttcgtaaact ttttcgcgga agtatttacg tacttcttct acgacttcat
     3301 ttcccagatt tgttcgtgca tcatacattg tcagtaagac tccctcgatc tctaattctg
     3361 gattaaagtg tttttggact aaacgaaccg tattcagtaa ttggcttaat ccttccaatg
     3421 cgtaatactc acactgaaca ggtatcaaaa tcgaatcact tgctgtaaat gaattgattg
     3481 tcagatggcc aagagaaggc gggcaatcga tcaaaatata atcatactga ctgcttactt
     3541 ctgctaaaga tccttttaat cttgattctc ttgccatcat tgacgtcaat tcgatctctg
     3601 ctcctgctaa ttggagagtc gcaggcacaa tactcaaatt ttcgtgttca gtgatcaaag
     3661 ttgcctcatc aattggcaat tcattgacta gaacatcata gatgtctctt gtcacatcag
     3721 gttttctgat cccgacacca ctggtagcat ttccttgtgc atccatatcc actagcaata
     3781 cttttttgcc aaggctagca agacaagcac ctagattcac cgtcgtcgtt gttttgccga
     3841 cgccaccttt ttggttcgca acagatatta tttgtgccat ctcttctcct cctatttgat
     3901 tggctgctta ttaggcagtc cgggctttct tgggtatttc tttggggttg gtttcttttt
     3961 ttgaataacg atgatatgcc gttgatcaga gacattcggc aaggtaaccg cctcttcttt
     4021 gaggaattta ccacctaata aagcaatcgc ttgttttgct tctgtcaatt cctcttcact
     4081 ttttgctgct ttcaatgcat aaaaatagcc ttcttgttta gccaagggca aacacaattc
     4141 cgctaacaca ttcaaacgag caaccgcacg agcagtgaca aggtcaaatg atgcacgaaa
     4201 gtgaggattt tgcccaaatg tttctgcccg gtcatgatat aaggaagcat cgattcctaa
     4261 taatcgggtt aactcagata agaaggttat tcgtttattc agagaatcaa cgatcgttat
     4321 ctgcagttct ggaaaggcaa tttttaaagg tatgctagga aaccctgccc ccgaaccaac
     4381 atcgcataaa gataaaggtt gcgtaaagtc tacttcaaat gctaatgtaa gtgaatcgta
     4441 aaaatgcttc agatatacgt ctttttcatc cgtaatagct gtaaggttca tcttttcgtt
     4501 ccattcgact aatgtccgat aataaatagc gaattgattc atttgatgat cggtaagctc
     4561 tatccctttt tctgccaagg attggcgaaa ttcttctggt atcataaata ttcctttcat
     4621 tgtgaaacag caattgtttc acagattctc tccctacttt aacgcaaaat ctctagacat
     4681 gcaatccttc catatggatt cttaaaaatt tgaaaagtaa gaaaagccga tagacttttt
     4741 ctttctagta tgactcataa aatatacaca gaagagagaa ctagctttga gcaatttctg
     4801 caaaaactag ttctctcttc tatttattat tacgtattac gctggttcac tgactttcgc
     4861 aatcttccct tgttcgatat agaccattag gatacttagg tctgcagggt ttacgccact
     4921 gattcggcta gcttgggcaa ttgtttctgg ttggattttc tgcaattttt gttttgcttc
     4981 tgttgctaat ccgttgattg ctgcgtagtc gatgttttct ggaatctttt tcgcttccat
     5041 gcgttttaat ttttctactt tttccatggc ttttttgatg tagccttcat atttgatttg
     5101 gatttctact tgttcgattt cttttcgtgt caattcttca ttttctggaa taaaccgtag
     5161 aagttctgca taagtaatct caggacgtct taaaaagtcg cttgctagta caccatcttt
     5221 taatggtgcc gcattaatcg ttgctaaaaa gtcttggact tcttctgttg gtttgattcg
     5281 tacattcatc aaacgtttga tttcttgttc aactgcttct tttttcttca aataattctt
     5341 gtattgatct tcttttacta agcctacacg gtgacccatt tctgttaatc gcaaatctgc
     5401 attatcatga cgtaaaagca gacggtattc agcacgtgaa gtcagcaaac gataaggttc
     5461 gttcgttcct tttgttacca aatcatcgat catgacaccg atgtaaccgt cacttcgttt
     5521 gatcactaaa ggttcttttc cctgaacttt caaagctgca ttgataccgg caatcagccc
     5581 ttgtccagcc gcttcttcat aaccgcttgt accatttgtt tgtccagctg tataaagatt
     5641 ttcaacgact ttagtttcta atgtcggacg gagctggtgt ggctctacta cgtcatattc
     5701 gatagcatat cctgtacgca tcatttctac tttttctaat ccagcaatag aatgaagcat
     5761 gttgacttga acatcctctg gtaaagaagt agataaacct tgaacataga cttcttccgt
     5821 atttaagcct tctggttcca aaaataattg atgacgtggt ttgtctgcaa aacgcacgat
     5881 cttatcttcg atggatggac aatagcgagc gcctacccct tcaacgatcc ctgtaaacat
     5941 tggggcacga tgtaagttgt ctcgaataat ttggtgagtc gtctcacctg tataggtcag
     6001 ccagcaagat tcctgatcta gtttataggc actatctggt gtgctgaagc taaaatgatt
     6061 cggctctttg tcaccaggct gctcttctgt aacagaatag tcgatcgtac ttgattttac
     6121 tcgaggcggt gttcctgttt tgaaccgttc gatagcaaag ccaagttctt ttagatgatt
     6181 cgctagtcca atagaaggtt gagagttatt cggacctgat gaatatttca attcaccgat
     6241 aatgatttct ccgcgaagtg ctgttccggc tgtaataaca actgctttgg cccggtagct
     6301 cgctcctgtg gacgtcacta ctccatgaca aacaccatct tcgacgatca gttcttcgac
     6361 gattccttgt cttagcgtca aatttggttc tttttcgatc gtatgtttca tttctgttga
     6421 ataagcatgt ttgtctgctt gtgctcgcaa tgctcggaca gctgggcctt ttcctgtgtt
     6481 caacatacgc atttggatat aggttttatc gatattctta cccatttctc cgcctaacgc
     6541 atcgatttct cgaacgacaa cgcctttagc tggtccgcct acagaaggat tacatggcat
     6601 gaatgctacc atgtccaaat ttattgtcag taataatgtt tttgatccca tacgtgcagc
     6661 agcaagtgca gcttctgaac cggcgtgtcc tgcgccaaca acgatcacgt catacgcatc
     6721 tgcttcataa tgttccaatc gtttgttcct ccttttattt tcctaaacaa aattgactga
     6781 ataattgagt gattagctca tcttgtacac tgtcgccgac aacttctcct agatagtccc
     6841 agcaacgcgt catatctatt tgaacgagat ccacaggcat tcctgaatca atacctgcaa
     6901 tgacttcttc aagtgagagg gaagcttttt ctaacaacgc gatatggcgg gtattagaaa
     6961 cataagtagc gtctctttct cctgtttctc cgctgaagaa taaatcagaa atagctgatt
     7021 ccaaacgatc tagtccgtcg tttttagcta cggaaattga aaaaactggt tcattttcga
     7081 tcaatttctt caatttttct tgttccagct gtgctggcaa atctgtttta tttagaagaa
     7141 tgatccgttt caatccactt gtcgcttcca ataattgttc atcttctgct gtcagtggtt
     7201 cgctttggtt tagaacaagt aagatcaagt ctgcttctgc taatgctttt cggcttcttt
     7261 cgaccccgat ccgttccacc acatcttctg tttcccgaat tccagctgta tcgattagct
     7321 tgagcggtac cccccgaaca ttgacatact cttcgattac atctcgagtc gttcctgcaa
     7381 tatccgtaac gattgctttt tcttctctta acaaatggtt taacaaactt gatttgccga
     7441 catttggccg gccgatgatt gccgtactta acccttcccg cagaaccttc ccttgcttgg
     7501 atgtagcaag tagcgcttgg attctttgtt ggatcatctg cgctttttca agcaataact
     7561 tcgtagtcaa ttcttcgaca tcatcatatt ctggataatc aatatttact tctacttgtg
     7621 ctaatgtctc taaaatctct tgtctcaatg agcggatcaa agcagacaga ttgccgtcta
     7681 actgattaag agctagtccc attgctttgt ctgtttttgc ccgaatcaaa tccatcacag
     7741 cttctgcctg tgaaaggtca acacgaccat tcaagaatgc tcgttttgtg aattcgcctg
     7801 gctcagctaa tcttgcacct tctcttaaca atagctgcaa aatttgattg actacaacga
     7861 tcccaccgtg acaattgatt tctactacat cttcccgcgt aaatgtcttt ggtgctctca
     7921 tcacggagac catgacttca tcgacaagct gattgctctt gggatcaacg atatgtccat
     7981 aatggatcgt atgacttggt acttctgata atcgtttgtt tccagattga taaactttat
     8041 ctgctagttg cacagcttga tcgccactta aacgaacgat actgattgca ccttctccag
     8101 gtggggtaga aattgccgca atcgtatcaa attctaaagt aatttcagcc attttttcac
     8161 ttcctttttc attttgcgca caaaaaaagt gcccactcca ccctttatgc acaataaacg
     8221 gtgaacttcg cactttgact gcttgttctt gattaaattc tggtcataga ttatcacact
     8281 tgctataagg aatcaagtgt ttaaaacaat tagatgaagt tgagcctgtt ctgcttccat
     8341 taccttatat ttaattgtcc ttggatgtct ctttgcgttc tttggtttat tcgaagagaa
     8401 gatactgtat aaacaaggct ttatctttct taaaaaaaac ggtacactaa atggtagcgc
     8461 tatctaaact atacactttt gaaaggagaa aatatgtgag cccgttcaga tctgctgcat
     8521 tggaagaaga aagtgaccct cggatcaacg atgtcgtttt atctacaggg aatatcaacc
     8581 agaaatatat cgtccgcgat atcgtttttg ttgtggaatg cttagaagct gatttgtttg
     8641 acccatcttt tcccaaagat gaccttttct taacaacgaa gaggaaatta aagacgaagg
     8701 tacttgaata tggtgcgaat gcagtaatca actgtcattt tgagtatcag caaaaaaaac
     8761 gcgacggaaa gtattttctg gaactttttg cttatggaac agtgatccaa tttatccaaa
     8821 caacgattgg ttaaatgaaa cagacaaaaa aagcggatca aaactgaaga cagtttcgat
     8881 ccgctttttt ctatcaaagt tagtgactac ttcatcgtat actctgataa gaatgatttc
     8941 tagtaaaata tacttggttt gaaaaacttt gtcaacgtga tttattcaaa aagtaatgga
     9001 atcaataata ttttttagca ggttctacta ctaaataacg ataaggttcc tcaccttccg
     9061 aatgtgtttt tacatgttca tctttgctta atgcagcatg gatttgttta cgttcaaatg
     9121 caggcattgg ttctaagaaa acaggctgcc ctgtccgttt tactttttca gccgtgcgat
     9181 cagctaaacg ctgaatgatc tcttggcgct tttcacggta attgcctaca ttgactacta
     9241 cagacagctt atttcttgct aaacgatgga tgaagacttg tgccagatat tgcaatgcat
     9301 tcagtgtttt tccatgtttc ccaatcagta tgccttgctt ttgtgtttct aaatggaaaa
     9361 caaccatatt atttgattct ctttccattt tgacaagagc tggagcatca agctgttttg
     9421 aaatttcagt aagataaact gccaattccg ttaaagctgc ttcatcacct agctcactta
     9481 cttcttctgt tttttcttca agaatcgttt cgttgactac ctcgatctca gctgttggtt
     9541 cttcttccaa aatttcttct acagtctgtt ctactgtttc agaaactttt tcgctgatac
     9601 ttggctggat cgaaagccgt gcattctttc gacccattcc taggaaacct ttttttcctt
     9661 cttccagtat ttcgatttcg acttggtctt tggccaaacc caaatcagcc agacctagtt
     9721 ggacagcttc gtctattgtt gcagcttcat aaatcggcat tagcattacc tcccctatta
     9781 tttccgtttt ttcttaggat tcattgcttt cttaagagca cgttctcttt cgcggattct
     9841 ccgtgcttct tcttcacgct cttttcgaat tttgaaaggg ttgttgatca gcagcgtttg
     9901 tacaacttgg aaggcattgg aaactaccca gtataatgaa agaccgctgg ctaaattgac
     9961 acccatcact aaaatcatta caggcatcac gaaattcatg atcttcattg atgcattcga
    10021 ttcaatttga ctcatgctag ataaatacgt acttgcaaaa gtaaagattg ctgctaaaat
    10081 cggcaaaata aatgttggat ccgcgttccc taagttcaac cataagaaat gcccttgttt
    10141 caactctggt acacgtgaaa tagattgcca taacgccatc aagattggca tttgtactaa
    10201 taaaggaaga caaccagcat atggattgac gccattttct gcatataaac gctgctgctc
    10261 ttctcttaac ttactttgtg tttccggatc tcttgaacta tattgttgct gcagtgcttt
    10321 taatttcggt tgtaattctt gcgttttacg catgctcttt gtttggaaat gcatcaacgg
    10381 caataggatc acgcggatga tcagtgtaaa taaaatgatc cctatccctg cattaccaaa
    10441 tgacaacgcc ttgatcgcct cagcaaagta ataaacaata taacgatccc aaattcctgt
    10501 actctgtgcg ctgacttccc cagttccgca accggataag acgatgacca aactaacgat
    10561 accggccatc aaaagtaaac gtttgtattt cttcacttac tcttttcctc tctgtcgatt
    10621 atttttgcaa gttttaagac gtggactaaa ttggagcgaa cctcatcata agtcaacccc
    10681 ttcacacttg gtctagcaat cacaataaaa tccaactccg gatcaatttc ttcttttata
    10741 gaataaactg ctgctcggat ttttcgtttg acttcattgc ggtttaccgc gttgccgacc
    10801 ttttttccta cagataaacc aactcgaaaa tgaggctgtc ccggtttttc caaacggtaa
    10861 acgacgaatt tgcggttagc aaaagaatct cccgtatgga acacggcttg aaactctcgt
    10921 tcttttttta ctcgatacgc ttttctcatt agttttcttt ccttttctat ctctcactta
    10981 cgctcataat cataccataa ttacgattgt cctttcatca tttccctaaa aaagagtata
    11041 aaaaaaacca ctgaaacccc agtggcctaa gctgaaagaa cttttcttcc tttgcgacga
    11101 cggctagcta acacacgacg tccattttta gtactcatac gtttacggaa tccgtgaact
    11161 ttttgacgtt tacgtttatt tggttggtat gttcttttca tatattccac ctccaaaatg
    11221 ttcttatcta ttttataaac acagacatac tttgatagta taacgacaaa gtagtgattt
    11281 tgtcaatata aaatagaaga tcgattgtta aaacttatcc acaagattat ttttttctat
    11341 gggagtccta tgtggaaaaa gttatgcaca gggattatcc acagataata aatggctggt
    11401 tttacacaga aaacacacgt gtaaaaaaag taatccacaa tcaggtgatt tcctgtgtat
    11461 tccgtctcct aactattggt atatctatat tttttatcca cagatgcaag aggaaatcta
    11521 ttgattttta cgagttttcc acctgtgcat tagtggtgtg gaaaactttt ttttgacaac
    11581 tgtgtttttc tcttttttct ttttgtcgat tgtgtggata attgtaatta aaaaattttt
    11641 ttcttcggtt tctgtggaaa actattgaaa aaaatgctaa attagtacta gttattcact
    11701 ttttaatgaa ggaggaggcc ttatatggta tccctcgatg ctttatggaa tgaattaaaa
    11761 gcaacatacc aaaaggattt atcgcctgct agctataata catggatcga aacagcgaac
    11821 cctcgaaccc ttgatcaaaa ccagctcgtt gttgaggttc ctagcaagat tcataaagaa
    11881 tattgggaaa aaaacctggc gacaaaaatc gttgaagtcg gttatatgtt atcaggaaat
    11941 gagattatcc ctcgctttat tactggcgaa gaagcagagc aagaagaagt tatagaagaa
    12001 aaaaatccaa aagttgtggc acctagtccg ctaaaaaaag ccatgctgaa ccctaaatat
    12061 acctttgata cgtttgtcat tggtaaagga aaccagatgg ctcatgcagc agcgctcgtt
    12121 gtagcagaag atccaggttc tatttataac ccgctcttct tttatggagg cgtgggactt
    12181 ggtaagactc acttgatgca tgcaattggt catcaaatgc tgcaaagtca gcctaatgcc
    12241 aaagtaaaat atgtaagcag tgaaactttt gctaatgatt tcatcaattc gatccaaaac
    12301 aaaacagcag aagagttccg tcaagaatat cgaaatgttg atttactgct agtggatgac
    12361 attcaatttt ttgcagaaaa agaagctacg caagaagagt ttttccatac tttcaatgcc
    12421 ttgtacaatg aagggaaaca aatcgtacta accagtgatc ggttgccaaa cgagattccg
    12481 aaactgcaag aacgcctcgt ttctcgcttt gcatgggggc tgtctgttga tattacaccg
    12541 ccggatcttg aaacaagaac agcgatttta cgtaaaaaag cggcggctga acgtctagag
    12601 atcccagatg acacactaag ctacatcgca ggacaaattg attctaacat tcgtgaatta
    12661 gaaggtgcgc ttgttcgagt gcaagctttt gctgcgatga atagtgaaga tatttcgact
    12721 agtctagctg cagacgcact gaaaacgtta aaatcaggaa aaggacatcc tcaattgtca
    12781 atcctgcaga tccaagaaga agtggcaaag tattatcaca tccaattgaa agatatcaaa
    12841 ggaaagaaac gcgtcaaatc tattgttgtt cctagacaaa tcgctatgta tttggcacgt
    12901 gagctcacag ataattcttt accgaaaatc ggagctgaat tcggtggcaa ggatcataca
    12961 acggttatcc atgctcatga aaaaatccag cagctgatgg attctagtgt cagtatgcaa
    13021 aatgaagtat cagaaatcaa aaacttttta ctaaattaat ttgtcgattc tatcaactga
    13081 aaatagcatg tggataaaag aaaaaaacgt tcaaaagtta tgcacacttt ttccacagct
    13141 gttttttcgt ttctctctta cgttttttga gttttccaca gaatcaacat gccctattac
    13201 ttttattatt ctttttaact aataataaat aaacaaacat tcaaaggaga tacatcatga
    13261 aagttacttt aaaccgagct agctttatgc aggaattgca aactgttcaa cgagctattt
    13321 caagcaaaac cacgatccct attttgacag gtgtaaaaat cacactgaca caagaaggtt
    13381 tgactttgac ggggagcaac gctgatatat caattgaaac ttttttgtct gttgaaaacg
    13441 aaaaagcaaa tatgcaaatc gaatctactg gttccattgt tttacaagca cgtttcttta
    13501 gcgaaatcat tcggagactt cctgaagaaa catttacttt agaagtttta gaaaataaac
    13561 aagtagcgat tacttctgga aaagcgaatt ttatcgtaaa tggattagat gcagataact
    13621 atcctcatct tcctgttgtc gaaagccata accagatgaa attacctgta cacgtattga
    13681 ctaaactaat caacgaaaca gtttttgctg tctcacaaca tgagagtcgt ccaatcttga
    13741 caggtgtgca tttcatttta tctgataatt ctttattggc tgtagctact gattctcacc
    13801 gtctaagtca acgcgtgatt ccagtagaac aagcggctga tcattttgat attgttattc
    13861 ctggaaaaag tttgatcgaa ttatctcgct cattaacaaa tgaagaagaa atcgtcgaaa
    13921 tcagcattat ggaaaaccaa gtgttgttca aaacagaaac gatgtatttc tattcccgtt
    13981 tgctagaagg aaactatcct gataccaatc gtttgattcc ttcaagtttt aatacagagg
    14041 ttgaattttc tgttcctagc tttttagcgg cgatcgaacg ggcttcttta ctttctcatg
    14101 aaggccgtaa caacatcgtt cgtttatcta ttcgtccaga tgcggttgtt ttatacggaa
    14161 attcgccaga aatcgggaaa gtagaagaaa gcttgagtta tacagcaagc agtggcgatc
    14221 cattagatat ttctttcaat ccggattata tgaaagcagc gttgcgtgca tttggagata
    14281 tgagcatcaa agtgaaattt atctctgcta ttcgtccatt tacattggaa ccaacggaag
    14341 atggcgttca atttattcag ctgattactc ctgtacgtac aaattaacaa tgattttcat
    14401 tgttcttgaa aatttaattg aaaagaaata gaaaaaataa aattttaaac agaaggccct
    14461 aaaatctctt ttaaggcttt ctgtttattt ttgtttaaat cacctttttt agattaaatc
//
LOCUS       DANKHL010000061        14503 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000061 DANKHL010000000
VERSION     DANKHL010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.064"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            379..711
                     /locus_tag="PW101_001299"
     CDS             379..711
                     /locus_tag="PW101_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2513705.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            1008..1163
                     /locus_tag="PW101_001300"
     CDS             1008..1163
                     /locus_tag="PW101_001300"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDL2513706.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
     gene            1439..2779
                     /locus_tag="PW101_001301"
     CDS             1439..2779
                     /locus_tag="PW101_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2513707.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(3020..3427)
                     /locus_tag="PW101_001302"
     CDS             complement(3020..3427)
                     /locus_tag="PW101_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL2513708.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(3422..4626)
                     /locus_tag="PW101_001303"
                     /pseudo
     CDS             complement(3422..4626)
                     /locus_tag="PW101_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180763.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            4752..4970
                     /locus_tag="PW101_001304"
     CDS             4752..4970
                     /locus_tag="PW101_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2513709.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            4991..5626
                     /locus_tag="PW101_001305"
     CDS             4991..5626
                     /locus_tag="PW101_001305"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2513710.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5819..6058)
                     /locus_tag="PW101_001306"
     CDS             complement(5819..6058)
                     /locus_tag="PW101_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2513711.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(6082..7605)
                     /locus_tag="PW101_001307"
     CDS             complement(6082..7605)
                     /locus_tag="PW101_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDL2513712.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            complement(7623..7745)
                     /locus_tag="PW101_001308"
     CDS             complement(7623..7745)
                     /locus_tag="PW101_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL2513713.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            complement(7775..8758)
                     /locus_tag="PW101_001309"
     CDS             complement(7775..8758)
                     /locus_tag="PW101_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDL2513714.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(8782..8931)
                     /gene="rpmG"
                     /locus_tag="PW101_001310"
     CDS             complement(8782..8931)
                     /gene="rpmG"
                     /locus_tag="PW101_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL2513715.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(8952..9329)
                     /locus_tag="PW101_001311"
     CDS             complement(8952..9329)
                     /locus_tag="PW101_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513716.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(9361..9540)
                     /gene="rpmF"
                     /locus_tag="PW101_001312"
     CDS             complement(9361..9540)
                     /gene="rpmF"
                     /locus_tag="PW101_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL2513717.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(9555..9824)
                     /gene="rpsN"
                     /locus_tag="PW101_001313"
     CDS             complement(9555..9824)
                     /gene="rpsN"
                     /locus_tag="PW101_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL2513718.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(10347..12113)
                     /locus_tag="PW101_001314"
     CDS             complement(10347..12113)
                     /locus_tag="PW101_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2513719.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(12073..13827)
                     /locus_tag="PW101_001315"
     CDS             complement(12073..13827)
                     /locus_tag="PW101_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2513720.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(13937..>14503)
                     /locus_tag="PW101_001316"
     CDS             complement(13937..>14503)
                     /locus_tag="PW101_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HDL2513721.1"
                     /translation="KTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGLL
                     SGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFG
                     YDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIW
                     GIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         4628 a         2818 c         2218 g         4839 t
ORIGIN      
        1 atgattttaa ttattaacgt tttttaattc gtttttagct taaaaataac ttttgtcaca
       61 gtctcactcc taaaattact ttatttacgt acgaagtggg caggaggtcg aatccagaac
      121 tgggccatgg tacttcaacg aggacttctc ctctagaatt gaaaagcatg cgatttacct
      181 tccgtaacga cggtagattt attcaactat gtgtccaatc aaaatttgtc tgatttcaaa
      241 aatagtcaag agaattttgg ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt
      301 gacaaaccct gatactaagt tgggctatga tacacttttc ttgactatca gttacgttac
      361 cggaaggaat gaactctatt gaaaaaaaca tatgatcgta aatttaaact aaagatttca
      421 caggatatcc ttgagaaaaa agtaactacc aagaaaattg ctgaagaata caatatctct
      481 cgtccaacaa tttcccgatg ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct
      541 ggacaaggaa aaagattacc tgataaagca gatttctaca ttcttgaaca ggaaaataaa
      601 cgactaaaag aggaagacga gtgttctacg aacacaaaga tcgatacgga tctccaggga
      661 tcactcaaaa actttacgat gaaggaatcg aaatcaataa acgtgttgta gctgttctta
      721 tgcgtgaaat gaatttatgc acaagaggat ttcatcgacg caaatcttct catggtagag
      781 aaaaaacaat caaagaaatg gtaaaaaaaa tgttgaatcg ataatttgaa cagaaccaaa
      841 ttgatgctgt ttgggtgacg gatattactt acattccatg taatgacggt cgcctctatt
      901 tgtccaccta tattgactta gcaactcgta ttctacgtta ctatatggtg gattcacaca
      961 tgaaaaaaga gattgtcgtc cgtccattac aaatttatta aggagaattg cctacggtaa
     1021 ttcattccga tagaggtagc cagcatcggt cttttattta tcaagagctg ttgaccgacc
     1081 accacatcac acattcaatg agccaaactg ggactcctgt tgacaatgca gtaattgaag
     1141 cgtttcatcg atcaatcaaa tgagaattaa tcgactccaa caaatataaa agtaaaatag
     1201 agatgaaggt gttaatccaa gattatttag aagactacta cccaaacaaa agaatccata
     1261 caaaatttat gatgactcct tgacaatttg aagcgaagaa aataagggat ctataatatc
     1321 tagtgttggt gacgccttct gttgccaata ctttttcttt tttctaataa acgcaaacag
     1381 acaccttgaa atcctttaga ataaagtcga cgaaaaccat tcaaaggagt gtttcaagat
     1441 gtcctattca gagtttacca aagagttact agatattcta gacctaaatc ttacctttca
     1501 tgaagatgcc tttcgcaaag agcgcattaa tgacgaaaca tgttttgttt ttgatggcac
     1561 attgacctat caaccagagg aatgcttcca ttgccactat caaaataagc aaacgatcat
     1621 taaatggggc tggaaaaaag tatccatctt attgaatgat gtcagtaact acaagaccat
     1681 tgtgcggatt aacaaacagc gcttcaaatg taaacactgt ggaaagactt ttttagcaga
     1741 agattcagtt agcgatcgtc gctgctccat tgcccgcaga gtcaaacaag ccattcttga
     1801 gctattaagt gagcctattt ctatgtcttt gattgcacgt atgaagcaca tttctccgac
     1861 cactgtcatt cgaatacttc gcagccttcg gcctaaaacc gtttctttga accagccgtt
     1921 accagaagtg gtgtgtttcg atgaattcaa atcggtgaaa aacgtctcgg gagccatgag
     1981 ctttgtcatg atggacggca agacccatca actgattgat attgtggaaa atcggcaact
     2041 caatccttta cgcgactatt tcttgcgcta ccctagaaaa gtccgtgaac aagtacgtct
     2101 agtggtttca gatttctata caccctatcg cacattggtg aaagagtgtt ttccgaatgc
     2161 ccgaatcgtc gccgatcgct ttcatattag ccaacacatc ggtcgggcct ttaccaacca
     2221 ccgaattcaa gtaatgaaga cctttaaaaa aggggatcgt cggtcgaagc acttaaagaa
     2281 atactggaga ctacttcaaa agaatgcgtg ggaattgaat ggtcagcatc gctactggcg
     2341 tccgtcattt cgagatcatc tgacagaagc ggagatagtc gatcggctat tatattatga
     2401 tgactcctta aagcgaggct acgaagtcta ccaggttttt ctttccgcta tcagaagaca
     2461 agatgttcct gaattcgtcg cgctcctaaa agaagactac aaggagttgc cagagcacta
     2521 tcaaccagtg ttcacgacct tcaagaaata tcgaacagaa atcaaacgag cgttgcgagt
     2581 tccctattcc aacggcccta tagaatgttt gaacaaccat atcaaagtac taaaacgaat
     2641 tgcctatggc tttagaaact ttcaaaacta ccgagaacga atctttttat atcgaggaaa
     2701 atatttcaag aaaactaaga acactaccca actgactaaa gccagaacaa caacacggct
     2761 agacaagata gctgtctagc cgtgtgagta tctcgattat tctattgaat ttcttcacca
     2821 acactcattg acgaagagcc aaaataagta atctaaccga ctgttaacta aaccattgca
     2881 ggtcccgtta aagatacttg tgcctgttgt caccgcttct ttttcttgtc cacaagcgct
     2941 taacacactt aataaagccc ctgctattat taataataat atctttttct tattaaaaac
     3001 ctcctcatct atttccagat tagtaaatgt agtaatcttt gctttctttt ttctcaatac
     3061 taataactaa cttatggggt aaacaaatac tcgtttgacc gggttttgag atccatccag
     3121 ttctaaccgc gatttgatca ggactattat cttctttaac tcgaattctt ccatctttaa
     3181 tctcaacaat attatattgt cccttcgcag gatgataatt cacgagttta tgattgatct
     3241 tatccaaatc gaagcgatcc atttccttgc catcaatttg aacgatggca tatttgatag
     3301 catcttcagt tgtttcattc tccttttcta tagagaatac aaataaggga ataaaagagg
     3361 gaataatcaa aatgccaata attatgacat cgcctatctt gacaccagct tttcttaaaa
     3421 atttcattaa cgatatctgg ctaaacatat tagaattttt ccatggaaat ggtgaaattt
     3481 ttcggaaatt atctgcatta agagaatcag ttaaacaacc ctcaagttgt tccttaatac
     3541 attgttgatc catcttcata ccaataaaaa ctagtttatt aatgcgttca ccaaacaacg
     3601 gatgccaggc ttccttcaat tcgggttttt ttgtataatc atttgacact caattttagg
     3661 taaactagct acccaataat ttaatgattc aatactttta tggctacccg atgtttcaaa
     3721 gatcgtcgaa aaatcactat gttgggcaaa ccaaagaaac ccttttgctc ttgtaatatt
     3781 gccaggaaaa tttttatccg taagatgggc aaatttttgt gcgtcaagga gtaaccgatt
     3841 tcgatagata aaacttgaga taccatattc ttctgtttca ggaatatgat tttgatagcc
     3901 aagctctaac tctttgaacc agcccattga ataggaagtc tcatctatat taaaaagatt
     3961 tgtatttaga attttcgata agtctacttc acctttaatt gtcctaatta ttttggcatt
     4021 ggactgtaac ttagcaatta gaccgttaat tttgtaaatc gtcttggttg acactaagtc
     4081 actattattt aaaagtaata tgttacaaca ctcgaattga tcaattaaca agccacgaat
     4141 atcctgttct tcttcaacta cattaccttc agcttttctt tcctgaatct tttcacccgt
     4201 ttcgaaatta tcccaaaaac gttttacatc cactactata ataatggcac caatctctaa
     4261 tcgttgggtc aaatcaatgt ctaaagggtt tttagctaat ataattgctt gaataattgg
     4321 aattggtttg ctgattcctg aagcttcaat aacaatttga ttcaagtcag aaaattgcac
     4381 taattgatct agttcaacaa tcaaatcttc gcgtaatgta caacaaatgc atccattcgg
     4441 catcgaaaca agtttttctc cagttctttt aaaacctttc ttgtcgacta ataccccatc
     4501 aacatttact tcccctatgt catttacaat gacagcaatt tcctttccgt gatcaccgta
     4561 cagaatatga tttagaagta tcgtttttcc tgaaccaaga tagccagtta agattgtaat
     4621 aggtattttt tcatagggaa cctctttcta taccttgaat ttacacttta agtgcaacac
     4681 ataaaaatga gtcataatcg atatccaact agtataagtt tgtcgagaac actcctagaa
     4741 ggaaaataat tatgagccag tattgtcatc ttactatact caaacgagaa agaatctttc
     4801 tgcttcacga agaaggattt tctattagta taattggcaa cgacattcag catagcccgt
     4861 ttactatttt acatgaactg agaagaaaca agaaaaaaac taatcacctt ccaaagcgga
     4921 aaaaaattat gcttatcgaa gaaaaaaatg cggacgttat cacattctaa atgatccgtg
     4981 agtttggtca attgttcgtc atttatttgt cgattttcag tggtcgccag agaaaatctc
     5041 gaatcgtttg aagcatgaac atatggactt ttcaattagt tttatcacga tctaccgtgc
     5101 aatttgcaat gatcttttcg atacagaacc cctctcacac ggcaataaag gcctgatccg
     5161 ttcattgcac catcgaggaa aaacaagaca tacaaaaaat catgtcgagt ctcgtgggaa
     5221 gatttgcctt actcattcga tccatggcca tccaaaatcc gcagatgata gaagtgattt
     5281 tggtcattgg aaagccgata ttgtagctgg caaaactagt ggtacttgtt tagtgacttt
     5341 gacagacaga aaatcatgct tccttttagc aggaaagaag gtccccagaa agaaagccgc
     5401 ttatgtgagc gagaaaatga ttgaactttt aagcagtatt ccaaatgagg cggtcaaaac
     5461 gatcactcca gatagaggaa aagaattctc caatcatttc gcagttacag aagtattgaa
     5521 taatatgccc ttttattttc cagatcctca tgcccttggc aacaatgaac aaattaaaat
     5581 acgaatagac tcattcgaga atacttgcca aaatcgaaag agatagctac ggtagataac
     5641 tcaacgataa atcagatgat tgaaaaactg aataacagac ctagaaagtg tttagattgg
     5701 agaacacctt atgaagtctt ctttgataaa gtgttgcgct tgatttgaca attcaagaaa
     5761 taataaaaag cagatcaaat ccaaataagg gaaaaatgat ctactctggt attatcaatt
     5821 aattggccaa gctattatgc cgttttgcta aacgtctact tcttccagta ttgaccttga
     5881 taaaacaaca atcaggatcc cgttttaata ctctatgaac tgtgtcaata ctaacatgta
     5941 aagttgcagc aatatcagct aatgtactcc catttaatct catttgcttt gcaaattgat
     6001 aacgccgata agcatcaata ttcttggaat ccatagacaa taatactttt cttaccatgt
     6061 atttcctcca aagattggat tttaatgggc catcatttct tgagcaatct catgctcaga
     6121 taatgaagct ggatagaaag taggccaatt atgcatttca ttataaagtt tttcttgact
     6181 ttctccacca aagaaaatat ggaaatgctc tgccttactt ggtgcaattc catgatcgct
     6241 aaattgaata tatttaaaac gaccagcacc tgcatcatca gtttcaaaat taaaacgaac
     6301 accacggttt cctttttcat aattcaaaat tttgtatcca acatatttgt atgtgtattg
     6361 atgatgttca ccgttgacta caaaatcaat ggtgttgtct ttaatgttga tagtatcaat
     6421 atctgtctta tagccagtag tataataatc tttgtactcg gcagctgtca tgtctttatt
     6481 gagttttgct ttgtaatcga aaacttcatc caaaattcca tcttttaaca acggataaac
     6541 agattgccat tcgcctgtat aatcactcaa ttcgcgatcc ttcacatcgg catctttaaa
     6601 gtacccatta gcaactgtct tttcatcctt cgcttctttt tctggaacta cctcttcacc
     6661 agcaaccata gttgttttct ctaacgcttt tagattgtct tccattactg aaacataatc
     6721 ttcaccatta tccatttgtt cttttgtaag actttcaaga ggattcaata cttccaattt
     6781 tatcccagct tcattggcca aagtttttgc gattttatca ttagcattct tttcaaagta
     6841 aatataattg atcttatttt ttttaacata ctcttttaat tcagctaatc gaccagaaga
     6901 tggctcttct tcaggactta gtccagcaat aggaacttgt atcaatccat aatccaaagc
     6961 taaatatcca aaggctgcat gttgtgtcac aaaacttttt tgtttcgctt ctttaagact
     7021 agtggtatag tcagcatcca accgtttcag tttagtcaaa tacttttcag catttgtctc
     7081 aaatactttt gcttttttcg gatacaattt aactaactga tccttgatat ttgaaacttc
     7141 cttaattgcc attttgggtg aaacccacgt atgaggatct agttcatgat ggtgatcttc
     7201 ttctccgtga tcatgatctt cttcttcact acctggcaat aaaatcatgt cttttgttcc
     7261 ttctactaca ttaggcccag ctttcttcca actttcttta gcttttggta cccatgattc
     7321 catgttttcg ttatgatata caaaaacatc agcatcatga gcttttgcca tatccttagc
     7381 tgagggctca tagtcatgag gttctgatcc cgctggaacc attaaatcca cgttcgcttc
     7441 atcgccgacg atgtttttgg tgaaatcata cattggatag aatgttgtta caatttctaa
     7501 ttttccatct ttatcattcg atacttttgc atcattagtg ctggagcaag ctcccaataa
     7561 tcctagagct gctatcccaa taagtcctac actgattttc ttcattcttt ttccttcctt
     7621 aattatgcta atttcttcat tttattttgt ttatgttctt tgataatttt taatgccctt
     7681 tttctagttt tgatattcgg gcttttaagt cgtcgatatg cactagatat atccgttttc
     7741 tccattagaa attaccccta atcaatactt atcattattt gctttccgcc agtaatttat
     7801 caaaactctg tttgatcctt tcttcatcca acatttttcc aattagaata atagtggttt
     7861 ggtctttttg tcgagtggtg tattgaaaat taacctgttc gtttacccct tgtatggcaa
     7921 ctataaaatc ttgatcttgc aaagcaatca atcctttaaa acgatacagg ctcccttggt
     7981 tggttaaaat cagccggtca attaattgga ataaaaaatc tttttttatg tcttgatctg
     8041 aagttaagag aattgattta aactcatgtt cttgatgatg ttgatgatag tgtttgccat
     8101 tatagtgatt ttctgatgaa tcactttcaa aaactaaggg atgattgaat ttttccaaat
     8161 tataaaaatc ctttttttga atcgtttcac tatgtgtgaa cattcgaata tcggataaag
     8221 ggttaatact tttgatttct ctctgtaatt tttttagtgc agataaattc aagttttgtt
     8281 ttctagacac aaaaatgcga tctgctatga caagttgctt aagggcttgt ggttctcttt
     8341 caataagttc aaaactgtca ctatcaataa cagtcaataa actatcgata tatactcttc
     8401 ctcttagttg aggcgctgtt aaaagtgttt gaatgattgg ttggggatct gctatacctg
     8461 tagtttcaat gatgatttgt gaaattggtt gttgatgttc ttctattgtt tccagtaaag
     8521 aaatcaatat tgctaataaa tcactgcgaa gactgcaaca catacatcca ttatttagtt
     8581 gtaacacccg ttcctcgcta tgaactaata atttgtgatc aatcccaact tcaccaaact
     8641 cattttcaat tataataatt tcttccctgg ggtagggtga gacttgcaag gcttgattga
     8701 ttagcgttgt ttttccagcc cctaaaaacc cagaaacgat tgtaactggt atgcccattt
     8761 tgtcaccttt ttaccactta gctatttcac ttctttgaaa attgcccgtc tgcgcaattt
     8821 tggcgaatac ttttttaatt ctaaccgttc agggttattc cgtttatttt tgctggtgag
     8881 atacagacgt tctccagttt cgacacattc caaaataata ttttgacgca taaatcttcc
     8941 tccttcaata cttatcgatc ttcaagatat gcttgaattt gttgtgcatc ttcaaaagtt
     9001 aactcgccag attccaattg tgaaactaaa ttttctttca tatcaacagg tatctcccaa
     9061 taaccatcaa tacattgaaa taactcctcg tcatactctt caaaaattgg atagattctt
     9121 aaaaaatcat caaatgataa gccttggtcc tcatagtaat ctttgactaa tccattttca
     9181 attgcctgtt gggtcggtaa acccgtttca gtaataattc ctctctctac catcgaacga
     9241 taaaaagtgc atttatctag cgattgctgg gtcgtttcaa gataatactt taatccttct
     9301 tcaactaaag gtgggtaaat aatttccatt ttgattccct cctaaaagtt atatattaaa
     9361 ctattttgaa gctcctttaa ttactttctt tccattatag taccctttta aagatacatg
     9421 atggctacgt ctataatcac cattcgcttc gtcaaatgaa atttctggtt ttactacagt
     9481 ttgatgagtt cttcttaacc gtttcttcgc cttagatgtt tttcttgctg gtactgccat
     9541 gatatttcct ccttttacca acttgctttt tttactccag gaatcaaccc ttgatgggca
     9601 agttctctaa acttaatacg ggacataccg aactttcgca tataccctct tggacgacca
     9661 tccgtctgat cacgaagcct cagacgattc ggatttgaat ctcttggcaa tttagcgaga
     9721 gcggtgcgat ctcctgcggc ttttaattcc tgacgtttat ctgcgtactt ttcaatcatc
     9781 gccatttgtt tctttgcttt agcaattttc gacttttttg ccatatacct ttatactcct
     9841 tttattttga tttagacatc tcaaatcgta atcgttacgt tttgacactg atactttacc
     9901 aacaagagtt gctcttgtca acaataaata acatgaaata aaactcatat gaatagataa
     9961 ctatttgaaa acgaatttat cggtacatag agataatcaa atcatttaaa agaatatgca
    10021 gtttaagctc tgaaaaataa gaattcactt ttattagtcc aatcaaacac aaataccgtt
    10081 ttaagtagct tttcctctac ttaaaacggc aatatgaaag aaactataac tttttattca
    10141 atcttttatg tttaactggt ttatcgccaa ttttttaaaa ctttgattat tccttatatt
    10201 ataagaatag acattaagtg aactaattca atcatactga ttctttcgtg tattcaaaat
    10261 tagatgctgt ttaataaaat tattcttttt gaaaaagaat tataactata tcccaaaaat
    10321 tgtaggttaa tttaaagcct atttcatcat cgtgattgag cttctttttc atagacgaat
    10381 ttctcagata gtttagtttt ctttatgaga tttttgtata tttctgacct tcgatacaac
    10441 tcactatgag taccttgatc aactattttt ccttgatcca tcactacaat tttatttacc
    10501 ttttcaatag acttcaagcg atgcgatata attagtactg tcttatcttg tattaaacga
    10561 tttaaacttt cctgaatacg tttttcattt tccgcatcta aactggcagt aatttcatct
    10621 aaaattaaaa ttggcgcatt tttcaaaaaa gcccgtgcta tggataggcg ttgtcgctct
    10681 cctccagaaa gtgaacttcc gttttctccg accaatgtat gataaccgtc aggtagtttt
    10741 tcaataaagt cttcgcagtt agctaaacga gctgcttttt tcacttcctc atctgacgct
    10801 tgactatttc caatacgtat attttccaaa atacttgtat taaacaaagt tacctcttga
    10861 aaaacaatag cgattttgct aaacaaagaa gctggagata cacgtttaat atcataccca
    10921 tcaattaaaa tacagccttc atcataatca tacagtctag acactaattt caagatacta
    10981 gttttcccgg aaccactagc acctactaac gcagtcactt ctccttgttt agccgtaaaa
    11041 gagatatgat ctaaaattgg ggtattgtta tcatatgaga aagagacatc tcgcaactca
    11101 atgtcaaaag atttcagagg actatctgat ccctcttgga tactggtttc cttcatagct
    11161 ctaattcgct gtattttagg agctaaataa aatatttcaa gcactgcttc tttcatagaa
    11221 tcgaaggaat cctttatttt tattgcggct agtaagtaac caacgacgta gagtatagtc
    11281 acttcacccg ttaacagtaa gtgaacacct actagaatga ctactgctaa actaacatat
    11341 gagaaaatag aggataaggc catgactgaa aaagtactta attctacctt taaatgaatc
    11401 ctttcactct cttccatttt tttgaaaagt ctgtcttgaa cttttttaga tagattaaag
    11461 ctattaatct cttgctgcaa ttctatagtt tcttgaaatt cttccgagtt ttctctcaaa
    11521 gtatcgtaat atttagtatt ggctttcgtt tgggatttct ttgataacaa gattaagaca
    11581 aaactaaata acgttggcaa aataacagct aatcccattt tgacattacc aaccaaaagc
    11641 atcactgaaa taaaagggaa aaacagagcc ataccgccgg ctttaggtat tgcatgactc
    11701 atcgcatgct caataccttc aacgtcagac atgatagttt gagataaatc tgataaatta
    11761 tgttttgaaa aataggataa tggtaaattt gacaaatcgt ctgcaatttg cattcttaaa
    11821 tgcgcacttt ctttataagt actattatat aagttttcat actccctatc taacaaccaa
    11881 tacatgaaaa gcaaagtaac gaacgatact gtaaaataaa gccaaggcgg ctttacattt
    11941 tctaaaacta attcgtctat cagcatcatt ataatgaatg ctggtaccat attaatgcag
    12001 tatacaaaaa agctagaaat agatgcttta accaaatctt ctgcgccctg attagttaaa
    12061 gcgaatttgt ttttataaaa acttttcatt gtcaagcctc caatcatttg cattctcata
    12121 attagtgacc aattgtttat acacttgatg tttagccatt aattcattat gatttccacg
    12181 ttctactatt ttcccttctg acaagacaag aatttcatct acagatttaa tgctggataa
    12241 tcgatgagct atcataataa ctgttttttc tttcatcaga tgttgaaaag ccttttgtag
    12301 ttcatactca ttatctggat caatagatgc actggcttca tccataataa caatctttgc
    12361 atcttttaaa attgctctgg caatagctat acgttgtttt tctcctcctg acagataaac
    12421 tcctttacta ccaattaagg tatgctctct ttcgggaaat ttttctaaaa tcgattcgca
    12481 accggctaat cttagcgcgt caaatacttt acttgctggc gcttgaggat ttgctataga
    12541 aacgttatcg taaatactct gattgaatag tttcgtatct tgaaagacaa aagcaattgt
    12601 gttaataatt gtgtcttttg aatagtgatt taaaggctta ccaccaattt taatctgtcc
    12661 cttatccaca ttgtagaaac ctgaaattag tttagctatc gtactctttc cgcttcctga
    12721 ttttcctact aatgcataaa ttcttttttg ttctaaaact aagttgaaat tttctaaaac
    12781 acactcttct ttgtatgaaa agctgacatt ttcaaatgca atgtcacaat ttttaatctg
    12841 tgtctcgttc ccatatttta gttggtcttg cttcatttct aaataaaggg agtctagtgt
    12901 ttcgactgca tatcttgctt caaacaagtg catacttaca tacattattc tcatgaaaga
    12961 aacaaaaata attccactta aaaaaaacaa catcaacaag tcaacagcta atatcttggg
    13021 agtggtaaga gaagttataa aaaaggttat aggaactatc aaaatgcaaa ctatgccaaa
    13081 aaatagccat tgaaaaagaa caaaaggtgt tttcccactc aaagaatact tatatgcaaa
    13141 cttagcataa tcgttaattg ctttattcaa ggctttaaat gatgttactt tagcaccaaa
    13201 tatctttata acttgaattc ctcggatata ttctaccgtt tcagagctta acacatctaa
    13261 cgattcttga tatttttgaa taaaatttgt attctctgtc attttcttta acaagaatac
    13321 accaattact aacatggcga ttagaaccaa tccaatttta aaattaatga aaaagcctaa
    13381 ggcaactgat aaaacaggtg ttactatggc ttgagcacta tctggaatca agtgagcaat
    13441 tgccatatgt gttttagccg cgttgtcatc aattgtcttt cgaatatatc ccgaagaatt
    13501 taaatcaaaa aaacgaaaac ttgcttctgt taaaccgcta attccctttt ttctcaaatt
    13561 agtctctaag cgaaacccaa ggatatgaga aaataagcca gaaaaaatat aaaataaagc
    13621 tcctaaagtt aaccaagcaa cagtttgaac cgcataactc ttagctaaat attcatctcc
    13681 tacaataatt agagacttaa gaaatttgta aatactgtaa tatccataga ctgttaatac
    13741 gacagaaaaa tcagaaaata aagtggctaa ccatccgtag tatttcattt tagggacata
    13801 actaaataat tttttgtata ctttcatttt ttcctccccc gcattcaatt tcttctctta
    13861 ttcgtttaca actgttaatg ctttccgtaa tagcgtaaat tagagatgta caaacagata
    13921 gaaggtaaac tacgttctat tttttgtatt tttttaagac ttttcttcca atcaatacac
    13981 caatcataga taaacttgct acaactaaca ctgaaatgat tccccatata cctaccgcat
    14041 agtggatcat tgcatccatt gttgcagcag attgtccttt atctatccaa tcttgacgaa
    14101 aacgatctgt aaagaaccat ataggaatcc actgtccggc agcactgcct aattgcatta
    14161 gagcccaaga cgcaacttga ggaattgctc gatcatatcc aaatacagat agaataatat
    14221 cagctaagat cgccatacta atcgaactga taaaccaagg taagtacgcc ccgcccatta
    14281 acgtaaaaaa taacaataaa acaattgtgt aaatagtaaa aattcctttt ctagggtagt
    14341 tatagctaat ataaacaaat atgattccag ataataatcc aaaaatccct ggggatatgc
    14401 tgtgaccaaa cgccccacca atactactaa ccattattga aaaaaagtaa attactaggg
    14461 aaataactgt agctaaacca ataaaaatat aatcttttgt ttt
//
LOCUS       DANKHL010000062        14233 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000062 DANKHL010000000
VERSION     DANKHL010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.065"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            3..371
                     /locus_tag="PW101_001317"
     CDS             3..371
                     /locus_tag="PW101_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDL2513722.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="PW101_001318"
     CDS             551..1492
                     /locus_tag="PW101_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDL2513723.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1561..2292
                     /locus_tag="PW101_001319"
     CDS             1561..2292
                     /locus_tag="PW101_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDL2513724.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="PW101_001320"
     CDS             2436..4616
                     /locus_tag="PW101_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDL2513725.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="PW101_001321"
     CDS             4636..5118
                     /locus_tag="PW101_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDL2513726.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="PW101_001322"
     CDS             5134..5895
                     /locus_tag="PW101_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL2513727.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /locus_tag="PW101_001323"
     CDS             6026..7360
                     /locus_tag="PW101_001323"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDL2513728.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7667..8152
                     /locus_tag="PW101_001324"
     CDS             7667..8152
                     /locus_tag="PW101_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDL2513729.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8145..8828
                     /locus_tag="PW101_001325"
     CDS             8145..8828
                     /locus_tag="PW101_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDL2513730.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8954..11599
                     /gene="polA"
                     /locus_tag="PW101_001326"
     CDS             8954..11599
                     /gene="polA"
                     /locus_tag="PW101_001326"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDL2513731.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11644..12480
                     /gene="mutM"
                     /locus_tag="PW101_001327"
     CDS             11644..12480
                     /gene="mutM"
                     /locus_tag="PW101_001327"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HDL2513732.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12444..13073
                     /locus_tag="PW101_001328"
     CDS             12444..13073
                     /locus_tag="PW101_001328"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDL2513733.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13125..13457
                     /locus_tag="PW101_001329"
     CDS             13125..13457
                     /locus_tag="PW101_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL2513734.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13769..>14233
                     /gene="nrdR"
                     /locus_tag="PW101_001330"
     CDS             13769..>14233
                     /gene="nrdR"
                     /locus_tag="PW101_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDL2513735.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENN"
BASE COUNT         4800 a         2490 c         3142 g         3801 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      421 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
      721 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1561 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     6901 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7441 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7501 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7561 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7621 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     7681 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     7741 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     7801 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     7861 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     7921 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     7981 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8041 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8101 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8161 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8221 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8281 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8341 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8401 cctactctct tttgaatggc ataatattag cggttactct tgcatactac gatattggaa
     8461 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8521 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8581 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     8641 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     8701 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     8761 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     8821 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     8881 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     8941 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9001 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9061 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9121 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9181 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9241 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9301 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9361 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9421 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9481 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9541 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9601 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
     9661 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
     9721 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
     9781 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
     9841 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
     9901 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
     9961 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10021 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10081 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10141 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10201 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10261 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10321 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10381 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10441 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10501 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10561 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10621 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    10681 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    10741 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    10801 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    10861 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    10921 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    10981 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11041 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11101 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11161 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11221 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11281 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11341 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11401 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11461 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11521 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11581 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    11641 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    11701 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    11761 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    11821 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    11881 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    11941 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12001 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12061 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12121 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12181 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12241 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12301 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12361 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12421 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12481 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12541 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12601 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    12661 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    12721 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    12781 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    12841 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    12901 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    12961 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13021 aaatacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13081 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13141 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13201 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13261 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13321 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13381 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13441 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13501 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13561 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13621 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    13681 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    13741 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    13801 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    13861 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    13921 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    13981 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14041 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14101 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14161 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14221 aaaagaaaat aat
//
LOCUS       DANKHL010000063        13878 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000063 DANKHL010000000
VERSION     DANKHL010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.066"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(143..1345)
                     /locus_tag="PW101_001331"
     CDS             complement(143..1345)
                     /locus_tag="PW101_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2513736.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1348..1542)
                     /locus_tag="PW101_001332"
     CDS             complement(1348..1542)
                     /locus_tag="PW101_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL2513737.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1589..2437)
                     /locus_tag="PW101_001333"
     CDS             complement(1589..2437)
                     /locus_tag="PW101_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HDL2513738.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2642..2992)
                     /locus_tag="PW101_001334"
     CDS             complement(2642..2992)
                     /locus_tag="PW101_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513739.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3587..4033
                     /locus_tag="PW101_001335"
     CDS             3587..4033
                     /locus_tag="PW101_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL2513740.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4657..5649
                     /locus_tag="PW101_001336"
     CDS             4657..5649
                     /locus_tag="PW101_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2513741.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5665..6549
                     /locus_tag="PW101_001337"
     CDS             5665..6549
                     /locus_tag="PW101_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2513742.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6598..8205
                     /locus_tag="PW101_001338"
     CDS             6598..8205
                     /locus_tag="PW101_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2513743.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8206..9690
                     /locus_tag="PW101_001339"
     CDS             8206..9690
                     /locus_tag="PW101_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDL2513744.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9867..10859
                     /locus_tag="PW101_001340"
     CDS             9867..10859
                     /locus_tag="PW101_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2513745.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10911..11780
                     /locus_tag="PW101_001341"
     CDS             10911..11780
                     /locus_tag="PW101_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2513746.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12200..13765)
                     /gene="guaA"
                     /locus_tag="PW101_001342"
     CDS             complement(12200..13765)
                     /gene="guaA"
                     /locus_tag="PW101_001342"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL2513747.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT         4254 a         2348 c         2864 g         4412 t
ORIGIN      
        1 ttgaaaaacg ttgatttatc ggcattcccc atccatatta tatttacgtc ttattcccac
       61 tcaatatgag tagcctcgta aagaagctat tatatcagtg ctttttcagc ctattcctca
      121 ttttggtagg gaaatggtag ggttagattt ttactgcttg atttccatta aagtttgcaa
      181 ctttagattt tgttgcacag gtaaatttaa tgtttcctgt catttgttct gcaattcctt
      241 catcattacg gctccataga tgagcgtagt gctttaaagt gatttcaggg ctagaatggc
      301 ctaaccgttg cgacacgacc agaatgtctg cgttaaactc attgattagg tagctaacat
      361 gagaatgacg taatcctttt ccttgtatta ggggtacctt agctagtttg gcataacgct
      421 gaataattcg ttgaacagtt gaacgataga gtgggaggtc agtgtagcta agaataaatt
      481 gttctacacc atgatttttt tgaataactt tccacttttt tagcacttca attgtatcat
      541 cgtccagaga aatgatccgc aagccgcttt cagtttttgt gtaaggtttc cgaataaaat
      601 ctttttgatt tgtcatttcc aaggtgtgat gaaccctcaa ctttttacgt ttgaagtcca
      661 cgtcattcca attaagtgct aatccttcgc ttacacgaac accagtcatg taatatagcc
      721 agatcattaa aaaacacata tgttcgtaga aatcatcttt ataaattttt gccaaaactt
      781 cttcaaattc ttcttttgtc cagtaaggaa cgattgattt tccttttgga attgcttttg
      841 ttcttttgga aatattctca ttcagaaatt gcagggtgac cgcatagtct aatgatttac
      901 gaaacatacc gtacataagt gaactgtatg cttgtgagta tccgctatcg ttcaataacc
      961 aaattcgata attttcacaa tctcgcacat tgatttcacg aagcttctta tcaccgaaaa
     1021 attcagtaat ctgaatcaat ccacactcac gagaactcca agtacttttt tgtacgctag
     1081 ctttgtagtg tggaacgtaa gactgctcca taaattcttt gtaagttaaa tcataattcg
     1141 caaatccatt tacaaccatg tattcatttt ttatacgcat gacttctttg tgtgcttcac
     1201 gagctgatga gaatcgtttt cctagagaat cttttgttcc tttcttctgg atcctctttc
     1261 cagtcacttg atctgttcct aactcaacta gataagaaaa gcgaccattt ttatcttgat
     1321 acacacaagg gtattttgtt ttcatagtta ttcctcctgt gaaagaggaa cacctataat
     1381 ttcttcaatt acttgtcggg gtactacccc aactctttta ttattataat atggatatcc
     1441 ctgatttacc attcttgttt ttgcttgtcg gattattgtg gtagcttgat gtgttttaaa
     1501 tcctagagcc tctaaatctt ttcgacaaat tgtatttttc attgtataaa ctcactttca
     1561 aagaaaacac tatcattggg taatagtgtt atttctttat ttttgtataa tgaacggtac
     1621 tttcttgaaa atccatcact tgttcaggac ttcccaactc atttaaagct aaagcattgt
     1681 aaaaaaatgg acgtctaact atatattcgc ttgtatatag tatatcttga ttcgtggaat
     1741 catagggttt ctttaccatc tttttggtga cagttggtag tttgagattc cgggacttga
     1801 aaaaagcttt tttcttatgc tctttcaatt ccagctcttt atcgaaatat ttgctgatat
     1861 agcgcccgcg attttcgata ctgtctacat cgatcttgtt gatcttaacg aaaccctgac
     1921 cccaaatcgt agttaaccgg ttaaatgaga taaaggggaa atcaaacaag ataatatggt
     1981 agtggattgc accgcgttgc tgcttttccc acgtggcgag atactttaaa tttgcttttt
     2041 tagttttata gaggtcataa ttcagtcgtt tgataaattt tttaaattca ttatttgcat
     2101 actcaatgtc ttgaatgttt tctttgaaag tgagagttaa aaatttcgtt tgattgtcaa
     2161 aattcgtgtc tacaaggcgg gcaatctcaa atcgcatact ttgataatgt ttctgtttgc
     2221 gttttaaact gtcatattgt ccttgagcgg ataattcatc atagtttttt cttttttcaa
     2281 gagtaatatc attttcataa atccaagcta atttttgttt tcgttccaga tttacttcgt
     2341 tttcgatcgt tttacgtttg ggagtaatga ttgcttcata atcataaatt tcaatatacg
     2401 tgggagtttc aatgattttc tgattataag cttgcaaaaa tcatcactcc ttgttgcgta
     2461 ttcgattgtc aaagaaccag tcattttctc ctgatgttga tctataaatc aagttaaggg
     2521 ggaaggcctg cgtagcagcc cttctagcct atggggtgtt gggcggtctt aacggacaga
     2581 aacggctctg tgggcgctgc cccgccgttt cgtcctaacc acccaacact ccatagtata
     2641 gttagttttg tgtagtggta ccttgttttc tgggaatcaa agttgccgta tcagcggttg
     2701 ccgataaccc ataactgaca aattgtccat tcgagcgtcc ccaaggtaca atcaccaaat
     2761 tggtaaatgt aattatggct aaaccttgtt gggtaagttc ttccggcgtg atcacaggat
     2821 gttcagattt gactttgaca ctgatttttt cgtagcgaag ttttggtagg atacattcgt
     2881 agctccaacc tagaatttcg ccctcctttt gccagcgagt gacattgacg acttggtatt
     2941 ctttttcgag tgtttctttt ggtaaactga gatcggtaat ttttattggc atcacaattc
     3001 tcctttttta tacgaaagtt tatacgattt gattaaaaaa tatagatggc catctacgac
     3061 ttgtattata tacgagaact tatacgtagt caagagattt tctaaaaaaa tatatacgat
     3121 tttttatacg agtgttataa tgaaaaaaat gataaggagg aatttgaata tgtttggttt
     3181 agtacaacct aataaagtga aggtgggcca acgaatcaaa gaaataaaag acggaatgtc
     3241 tctctctttt acagaattag ggaatcgatt aggtttaaaa aagccgacga tcagttccta
     3301 cgttcaaggc tatgcgttgg ctcctaagag tgtgatcaac caattatcta gtatcagcgg
     3361 aaaacctgtc ggctggtttt attttgggca tgtggaagaa tatatccgag agtatttgtt
     3421 tctcattggt cagaaaaaaa tagtggacga ttatcctgaa atcattgaga aaataaagca
     3481 agaattttat acgggcgagt tcaaaaatcc tgcttgggag aatgagctag gctatccttt
     3541 ggaggaattt atccaagaat gttttgtaga atattcgagt gaaatcatga aggactatgt
     3601 cgtacagatt acgaaagagg tacttgaaga atccaaaacg ctaaacaaac tatcatcaaa
     3661 agcaaaagaa gaagctgtca cgattctttc atctgggatc attgactata tcgagatgag
     3721 tggtgagatc aagtatggag agaaagatcg cattgcgaaa atggtgaaag aatcaataaa
     3781 aacctttgat gtgaaacaag acctgacatt taatgattcc tatttgattg gaaagttgat
     3841 caatattctt ggtacagatg aagatacgga gctgtttctt tcttctttat caatgatatt
     3901 tactgataag catttttcca ctcgatttgg tggggaagaa ttagtcgaaa tatttcaatc
     3961 gatgcgacca gcgttaatca aactctatcg ggagaaaaca ccagatgaat tttatgattg
     4021 gtttgagaag taaaataaga aaagtggctt agattacaag ttggtgaaaa aatttttttc
     4081 agcataacat cagtaatcta taaaggggtt gttgattata ctgtagaagt taagtactca
     4141 agagattttt tgtacgtaaa caaagcaaaa aagggtgcag tttcactaga aaatgtactc
     4201 ttttttgtga tttttcttag ttatagagtt tttttgaaac tttaaaaatg agaaaacata
     4261 ttgattttaa agagtgtaga catagcttct tttgctaaat agattaaact tactggtgag
     4321 tggtcttgaa catactttta aatcacattt tactgagttt tattggattt tataattcat
     4381 tggaaacctc gtacttaatc tgtttttatc tatcgactag aatctgtata tattccagct
     4441 atcaatgagg ccgcagaaaa gggtgcttaa aaataatctg ttgtttaaaa tattattatc
     4501 agaatactta gatacaaaat tctggcacaa agtaaaaaaa atattgactt aaaaaaatgg
     4561 atatggtaat ctataaacga aaatagtggt aacgtttcca aaatgcatct tcgttaaatt
     4621 tttatttttt taaacgaaaa tggaaacgtt tctaaaatgt attgggagga gattgaattg
     4681 gaaaagacat tgggaccagg tggtgagata agggcggtac aacgggataa aaaaaatatt
     4741 tcgaagtggg aaaaattaaa gaaacagttg cggcgtgata agtggatgta cttgttgctc
     4801 ttaccaggta tcatcatgac gtttatcttt aagtatgtgc caatgtacgg tgctgtgatt
     4861 gcatttaaag actataatcc tatgctagga attttggaaa gtccttgggt aggactagat
     4921 cactttaggg aatttatcag ttcaccaaat tttacgcttt tgctgagcaa cacattaaaa
     4981 attagtattt atggtttatt gttaggtttt tttccaccaa ttattttagc actgagcttg
     5041 aatcaaatta tcaataaaaa aataaaacag aaaatccagt tgattctata tgcgccgaat
     5101 tttatctcag tagttattat tgtggggatg ctgtttttgt tcctatccgc aaatggacct
     5161 attaatgggt tagtagaaaa ggttacgggg caaccaatta tgttcatgtc taatccagct
     5221 tatttcagaa cgatttatat tttatcggga atttggcaag gaatcggctg gtcatccatt
     5281 ctttacaccg caacgctggc gggagtcagt accgatttat atgaggcagc aaatatcgat
     5341 ggggcaaata ttttacaaaa gatttggcat atcgatttgc ctgcattgag gcctgtgatg
     5401 gtgatcagct tcatcttggc cgccggcggt attatgaatg taggatatga aaaagcctac
     5461 ttgatgcaaa cttcaatgaa catccctgcc tcagaaatca tttccactta tgtatacaaa
     5521 gtcggcttgc aatcaggtga ttatgcctac tctgctgctg ttggtctgtt caatatgatt
     5581 attaatgtcg ttttattact gattgtcaac aatataacca agcggctaaa cgatggtcaa
     5641 ggcttgtaat gaaaggagaa aattatgaat agtgctttat caaaaatcga ccgtcaaatc
     5701 ttatggttga atcgagtgat tgtaactttt ttagtcgtca tcaccattgt tccgctgatt
     5761 tatgtactgg tagcttcttt tatggaccca gctactttga tcaataaagg tataagcttc
     5821 aatccgaaag attggacaat acagggatat caacgggtat ttgcagatga ttccatttta
     5881 cgcggttttg taaattcgtt gttttactct tttagcttca gcttattgac tgtatttatt
     5941 acgatgatca ctgcgtatcc tttgtctcga cctaacttgg cggggaaaaa gattattatg
     6001 actttttttg tcattacaat gttcgtgggt ggtggcttga tacctaccta tttattggta
     6061 aagaacttgg gcatgttaga tagtgtatgg gcgattatcg taccaggtgc tataaatgtg
     6121 tggaatatta tcttagcccg tacctatttt agtgctttgc caaaagaact ttctgaagct
     6181 gctactatcg atggcgccaa tgacttgcaa atactgttca aagtcatgct tccattagcg
     6241 aaaccaatta tgttcgtatt gtttttatat gcctttgttg ggcaatggaa ctcatacttt
     6301 gatgccatga tttacatcaa agatcccgac ttacagccgt tgcaactcgt attaagaaat
     6361 attttgatcc aaaaccaacc ttcacaagac atggtgggag ccaatactgc catggcagaa
     6421 atgaaacaaa ttgctgagtt gatcaaatac gcaacgatcg tcatttctag tctgccatta
     6481 atcataatgt atcctttctt ccaaaaatat tttgataaag gtgttttagt tggttctgtt
     6541 aaagggtaag tattgttcac tatttgtatg aataaaaatt ataaaggaga ttttaagatg
     6601 aaagcaaaat ggatgctgcc aatcgttttt acttccgtac taattttggg ggcttgtgga
     6661 ggctcaaaag aaaagagtaa atcaagccct gattatgaac tagaaaatgt ctcttttcca
     6721 ttgaaagaaa aagtatcttt aaaaatgatg tctcaaagtg cccctttggc tcccaatgat
     6781 cctaatgaca aattgatttt ccaacgatta gaagaagaga ccaatctcca tattgattgg
     6841 acaaactaca gtagtgattt tgccgaaaaa agaaatttgg atatcgctag tggtgattta
     6901 ccagatgcca tcttcaacgc cgctgcaggt gactatgatt tattaaactg ggcggaaagt
     6961 ggcgttattg taccgattga agatttgatc gaagattata tgccaaatct gaagaaaatt
     7021 tttgatgaaa accccgaata ccgacaaatg tctactgcac ctgatggtca catttactct
     7081 atgccttgga ttgaagaatt aggcgaagga aaagagtcga ttcatacagt aaatgggatg
     7141 gcttggatca acaaggagtg gttggataat ttgggtcttg aaatgccgca aaccactgat
     7201 cagttgatga ctgtgttgga ggctttcaag acgcaagatc caaatggtaa tggtgaagca
     7261 gatgaaattc cttttagctt tattaacaat ggaggaaacg aagacttaaa atttttaatg
     7321 ggggcattcg gtctaggtga taatgatgac cacttagtcg ttgataacga tggtcaggta
     7381 caatttactg ctaacaaaga agggtataaa gaagccatca aatatttcaa tgaaatgaat
     7441 aataaaggtc ttattgataa agaggctttc gaacaagatt ggaatacgta tgttgccaaa
     7501 gggaaagaac ataagtatgg cgtgtacttt acttgggata aagcaaatat cacgggtgca
     7561 gatgatagtt atgacgtctt gccagtctta gcgggaccag atggaacgaa acatgttact
     7621 cgtacgaatg gcatgggttt ctcaagagat cgtttcgtaa ttactagtgc gaataaaaac
     7681 ttggaactta cagctaggtg gatagataaa atgtatgaac cgttacagtc tgtccaaaat
     7741 aactggggaa cctacggtga tgaagcacag gcgaatattt ttgaattaaa ggatggtatg
     7801 ttaaaacatt tgcctttaga ggggacagca ccgggggaac taagacaaaa aaccgaagct
     7861 ggaggaccat tagcagttct tgattcttat tacggcacag tcactactat gccagatgat
     7921 gcgaagtggc gtttagattt gatgcatgac acgtatttgc catatattac agaagacaat
     7981 atctatccac gtgtcttttt agaaaaagaa gaaacagatc gattagcaaa aatcgaaacg
     8041 gatatgtggg attacatcta tcgcaaaaga gcagagtgga tcaccggcgg taaaatcgat
     8101 gaagaatggg aagaatacct tgctgaattg gaacgtgtag gtttggaaga atggattcaa
     8161 ataaaacaaa ctggctatga taattttttg aagggagcac aataagtgtt agtagaagaa
     8221 acggcgattt tggataaagc aaatcgattt attgaaatga tgcgaggaaa agttaaccct
     8281 cagtatcgac aaaactatca tttttccgca cccatcgggt ggataaatga ccccaatggg
     8341 tttatatatt acaaagggca ttaccatctc ttctatcaat actatcctta cgatagtcga
     8401 tgggggccca tgcattgggg gcatgcgcgc agtagtgatt tggtccattg ggaagatcta
     8461 ccagtagcgt tagccccaga tcaaccttat gacagcggcg ggtgtttctc aggaagtgca
     8521 attgaaagag acggaaaact atatttattt tatacaggct gtcaagaaat tgatggtatt
     8581 agccatcagc atcaatgttt ggcggtctct gaagatggta ttcattttag caaatacagt
     8641 ggtaatcctg tggtagcaga aaaacagcta ggtgaaaatg ggctaataca agaatttcgt
     8701 gatcctaaag taattgttca caacgatggt tactatatgg tagtagctac taaatctaca
     8761 cagtctactg gtagaatagt gatgtttcaa tcgtcagatt tgtatacttg gcacttttac
     8821 tctgtgctgc tagaagggac ttcagagcaa ggaataatgt gggaatgtcc tgatttattc
     8881 catttagacg gacgagatgt cttaatcatg tctccgatac aaatcaagaa gaacggactg
     8941 gaatatcata atatcagttc taccatggct tgtattggtg aggtaaactg ggaaacaggt
     9001 cagatgatag ttgataattt ccatgaaatg gattttggaa tggattttta tgcaccacaa
     9061 acaacaagag atcctaatgg tggacgggtg atgattgctt ggatgcagat gtgggatcgt
     9121 acattgccta cacatgagtt aggccatctt tgggcaggct ctatgagttt gccccgagag
     9181 ttaagagtga aaaaaaatcg attggtacaa aaacccatct caactgtata tagtcattta
     9241 gtttatcaac aaggcagtga aaatattgca gtgactgaca catcagtcgt atttcgaaac
     9301 gcgctgtctg attcttcata tttacatcta gtcgcagatt tggagaaagc tgaagttttt
     9361 acaattgaat tactgcggtc taagacggat tgtctcaaac tagcctatga cactgctaca
     9421 caggtttttg aattggatcg tcagaaacta ggaagagagc ttacaggggc ggaagaagac
     9481 cctctgaatg tacgaaaagt tgaagtacct ttgattgata atcagctcgt tcttgagatt
     9541 tttagagata cctcatcaat tgaagtcttt attaatgggt tagaagttat gacggctact
     9601 ttctatgagc tggaaaaagc tcgagatatc ttgtttcatt cgatcggaga aagtcgtatt
     9661 caagcttttg aaacagcgag tgtgaaataa atgtttgatt gcttaggcga tgaatcaatt
     9721 atttaatagg ttttgaagga taatgttttt agaaaagaac agatagctag atttcccatg
     9781 gatttgctat tagtattttg aaaaaattag taatttatga caaaattcat tatactaaac
     9841 ttgtgcagaa atgtgaggga tcaactatga aagcaacaat gaaagatgtc gcaaaattag
     9901 caggtgttgg agtaggaact gtttcccggg tattgaataa cggttctgtt aaagcttcta
     9961 ctagagaaaa agttgaagca gcaatgacag agttaaacta tcaaccagat ttttatgccc
    10021 gggggttaaa gatgaagcat acgaacacgg ttgctttgat tctacccacg gtatggcatc
    10081 cattttttgg cgaatttgcc taccatgtag aaacagcatt agaaagacaa aactacaagc
    10141 tttatttgtg taactccagt ggtaatagtg aaaaagaaca agaatatatc cagatggtca
    10201 gccaaaataa agtagacggc atcattggaa ttacttattc agatatcgat gaatacattt
    10261 cgagtaatct gccttttgtt agtattgacc gttatttcac tgaagaggtg atttatgtga
    10321 ccgctgacaa tgcggccggt ggacgtttgg ctgcaaatga actttatcgc cgaggatgtc
    10381 agaatgtagc atatatcggt gggtaccaag aaacaccgaa tgaaacgaag agtcgtcgaa
    10441 aatattttga aatcgagtgc cgtcaactgg ggatttccta taaagcgtta gatatgttag
    10501 aacctttaga ccatctctca gaacgtgtaa caaatttttt gttagagaat cccgatatcg
    10561 atgggatttt tacgatcaac gatccaatgg cattagctgt cattgatatg cttgaaaaaa
    10621 tagggaaaaa cgtaccagag gatgtccaag ttatcggatt tgatggtcaa aagatgacag
    10681 aaggagatcc ttatctagtc tcaacgattg cccaacctac aaagcaaatg gcggaagtgg
    10741 cagtggcaaa attaattgct gtcattaatg gagagacagt agaaaaacgt accgtattgc
    10801 cgattgaatt tggcgaaggt gacacaacga aacctttagt gagaaacgat tttgcttagt
    10861 gcaaatacgt ttcttttttt gaaaatgagt gaatgaaagg tgtagataaa atgttatatg
    10921 gtggaataga agccggtgga acaaagtttg tctgtgggat tggtaatgaa caattacaag
    10981 tgatagaacg gaaaagtttt cctaccacaa caccagaaga aacgatggag caagtgtttg
    11041 atttctttga taagtacaaa ggaaatatcg catcaattgg tgtaggagca tttgggccta
    11101 ttgatattca aatcgaatca agaacttacg gatatatcac caacacaccg aagttagctt
    11161 ggcaaaatta tcattttgta ggagctttgg aaaacgcctt gtcggttccc atctattgga
    11221 ctaccgatgt aaatgctgct tgttatggag aatatatggc agggtatgga aaaggtaaaa
    11281 atagtgttct gtattacacc gttggtacag gtgttggtgg tggtgctgta gtggatggga
    11341 aaatagtcgc aggttttagt catcctgaaa tgggacatat gctcttacaa cgtcataatg
    11401 aagatcaatt tgaaggaatc tgtccttttc atcatgattg tttggaaggg ttagcatcag
    11461 gacctgcgat tgaaaaacgc tttggaagaa aagcccagac tatctcattt gatcatcctt
    11521 attggacgat tgaggcagat tatcttgctc agtgtgtgta taacacaacg ttactatatg
    11581 caccggaagt aataatttta ggcgggggtg taatgaaaca gccgcaacta ctcgagttga
    11641 ttcgccaatc gtttaaagaa aaaatgggtt cctacgtgga tacgcctact ctcgaagaat
    11701 atctactaac tccaaggctt gctgacaatg cgggcttgat tggctgccta gctttagcaa
    11761 taaagaaact caatcattag aatagtattt attagaacta ttcataacat accggaagtt
    11821 taaaagggta ttctgaaact taaatagtct caattcattc ttctttcttg agaatctgag
    11881 tagagacttt tattctgttt caaattaata taattacgaa atgatattga gtacaagaaa
    11941 gttcttttta ccagatcttt taaatcgaaa aatctgatat atatatttat agtactaaaa
    12001 atctgattag ataaaaagct aattcaatag gaaaataaag gtagtgaata aaagcaaagc
    12061 ccttgttagt agttaatcta acaagggctt tggtagggaa aatggtagga aaccatctta
    12121 ttttttacga aaatcaggaa ctttagtaat tgaataatgt tgatttatag gtattcctca
    12181 tccatattat gttggctctt tattcccact ccactgttgc aggtggtttg ctggtaatat
    12241 catacacgat acggttaacg tgtgcgactt catttacgat acggacactg attttttgca
    12301 agacttccca agggatacgg gcaaagtcag cggtcatgcc gtcaatagaa gtgacagcac
    12361 ggatacctac agtgtagtca tacgtacggc catctcccat aaccccaaca gagcgaatgc
    12421 ctggtaggac ggtgaagtat tgccagatgt cgcgatctaa accggcattg gcgatttctt
    12481 cgcgcaagat agcgtctgat tcacggacga tttctaattt ttcttctgtc aattcaccaa
    12541 ggacacgaat acctaagcct ggtccaggga atggttggcg ccaaacgatg ctgtcaggca
    12601 tgcctaattg ggtacctaat tcacgtactt cgtctttgaa caatgtgttc aatggctcga
    12661 tcagcttgaa ttgcatgtct tcaggtaatc cacctacatt gtgatgtgat ttgatggttt
    12721 gggcagtttc agttcctgat tcgatcacat cggtataaag ggttccttgt gcaaggaaag
    12781 aaacgccttc ttcaccggca agtttggttg cttcatcatc gaagacatat acaaattcat
    12841 ttccaatgat tttacgtttt tcttctggat cagacacgcc agcaagtttt gataagaaac
    12901 gttctttggc atcgactttt ataatgttca agccaaattt tccgccaaga ctttccataa
    12961 cttgttctgc ttcaccttta cgcaacaata cgtgatcaac gaagatacat gttaattgat
    13021 cgccgatcgc tttttgtaaa agaacaccaa caacagagga atctactccg cctgaaaggc
    13081 ctaataaaac ttttttgtca ccgacttgtt cacgaatttt tgcgatctcc atgtcgatga
    13141 agttatccat tgtccagtca ccagtacatt ggcagacatc tagtgtaaaa tggcgcaaca
    13201 agtcatttcc atattcggaa tggcgaactt ctgcatggaa ttggattccg tacattttgc
    13261 gttctgcatt ttggatcgat gcgatggggc aatctttgct ggtagcaacg acatcgaaat
    13321 cagtcggtgc ttgggtaact aggtcaccat gactcatcca aacggtttgt ttttcaggag
    13381 tgttttggaa aagggccgct tctgcagaag tgatttccaa ttctgctttt ccgtattcac
    13441 ggtttttggc tggtttaact tttccgccta atttataagt aatcaactgc ataccgtaac
    13501 agattcctaa aactgggatc cccaaatcaa aaatcttttc atcaatacca aaggccttct
    13561 catcataaac actgtttggt ccgccagaaa ggatgattcc tttagggttc attgccttta
    13621 cttcttctgc ggtaatgcgg tggctcatca actctgagaa aacaccgaac tcacgaatac
    13681 ggcgagtgat cagttggtta tactgactac cgtaatcaag tacgataatt tttggctctt
    13741 cgtcaaatcg tgttggtgaa gacaaatttg aataaaactt agtccgatat gcggcgcgtt
    13801 ctgtgcgccg catatttttt tagttttcaa agatcagacc ttgttgtaaa tagatccgtg
    13861 cccgaaagtt ataaaagt
//
LOCUS       DANKHL010000064        13837 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000064 DANKHL010000000
VERSION     DANKHL010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.067"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(315..1484)
                     /locus_tag="PW101_001343"
     CDS             complement(315..1484)
                     /locus_tag="PW101_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL2513748.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            1688..2863
                     /gene="mltG"
                     /locus_tag="PW101_001344"
     CDS             1688..2863
                     /gene="mltG"
                     /locus_tag="PW101_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDL2513749.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            2999..3478
                     /gene="greA"
                     /locus_tag="PW101_001345"
     CDS             2999..3478
                     /gene="greA"
                     /locus_tag="PW101_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDL2513750.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            3647..4378
                     /locus_tag="PW101_001346"
     CDS             3647..4378
                     /locus_tag="PW101_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDL2513751.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            4375..5442
                     /locus_tag="PW101_001347"
     CDS             4375..5442
                     /locus_tag="PW101_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2513752.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            5449..6081
                     /locus_tag="PW101_001348"
     CDS             5449..6081
                     /locus_tag="PW101_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2513753.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            6221..6886
                     /locus_tag="PW101_001349"
     CDS             6221..6886
                     /locus_tag="PW101_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDL2513754.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            6914..7207
                     /locus_tag="PW101_001350"
     CDS             6914..7207
                     /locus_tag="PW101_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HDL2513755.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(7275..8222)
                     /locus_tag="PW101_001351"
     CDS             complement(7275..8222)
                     /locus_tag="PW101_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDL2513756.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(8400..9203)
                     /locus_tag="PW101_001352"
     CDS             complement(8400..9203)
                     /locus_tag="PW101_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HDL2513757.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            9349..10263
                     /locus_tag="PW101_001353"
     CDS             9349..10263
                     /locus_tag="PW101_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2513758.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10311..10781
                     /locus_tag="PW101_001354"
     CDS             10311..10781
                     /locus_tag="PW101_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDL2513759.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            10866..11474
                     /locus_tag="PW101_001355"
     CDS             10866..11474
                     /locus_tag="PW101_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDL2513760.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            11608..11808
                     /locus_tag="PW101_001356"
     CDS             11608..11808
                     /locus_tag="PW101_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2513761.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            12562..13788
                     /locus_tag="PW101_001357"
     CDS             12562..13788
                     /locus_tag="PW101_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2513762.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         4703 a         2113 c         2793 g         4228 t
ORIGIN      
        1 cttctttttt attgcataaa attaaaaata agaacaagcc atgaatgatt tgctagcaca
       61 tattttagca atctaaagat actattttag atttaatagg ccctttgtca aataatgttg
      121 gtaaagagaa atcttagaat aatttggaca gaacagaaag aagaacacct ctttctgttc
      181 tgtttatttg gaattaaagt ctgtagcaaa aattgatttt tagatttgtc ccacactctt
      241 tcctacgttt agtcgagtac tttttttaag caaatgctat tcaaagaagt agtatttgct
      301 atcttttcat catatcagct atttgccaac ttagtagttc cttccatcag gtcatagatt
      361 tggtttctta gatcagcagt aggcagtttg atagttttgt cagcttttat attgctttcc
      421 atgatgatag catcctgtga atctttgcta aatatagcag atgtatcaaa agagtcgata
      481 tttccttgcg acaggatata attatctccg ctatataccc caccactata tggatgtcca
      541 ttcgcatttt cacgccataa tgagaagagt aagtctttgg aaactagttt tccagataaa
      601 acttgtgtaa acagcgtatt taaatcatag acggacatac tgatattacc agtccctaat
      661 tcgttatgga tctctgtttc agattctgtc agtggtttaa agtaatcatc ctctttagac
      721 atttgatagg atagtgcatg attggatgaa tttttgactt ggtcataaaa ttttgtatgc
      781 tgtaatccta aaggttcgat gatatcatcg gtcaataatt tttcatacgg ttgatttgtt
      841 aatttgatca aaattccagt caatatggta aagttcaatg aatcataatg agaggtctga
      901 taatctgtaa attttaagtg ctgaatcgca aattgaacga cttcttcatc agtcatcggt
      961 atggttggag aagcagttcg gtgcaagcct gagcgcatat acagcaagtc acggatagta
     1021 atttgctggc tgccagcaat ttgtggatag aattgagcca gcttagtatc taaagaaagg
     1081 tgcccacgct cgacttgttt taaaatcaaa agagtagtca atgctttttg aaccgaacca
     1141 atctgataat aaagagaagg atcatttggt tgttgcgtga gtttattcgc atagccatag
     1201 cctttttcca tgatgatgcg attatttttc tttacgtaaa ttgagccaat ataatcattt
     1261 tttcttaata gttgatcgat tttttcaacg agctgttgtt cattggtagt cagtatcaac
     1321 tttttttgag ctgattgagc atgggcaact atctgagagt gttcattatt gtaaaattgc
     1381 catccaagaa tagttcctgt agagaacgca ctaaggctaa acaacacgac aacgatcatt
     1441 aaaaatgaat attttctttt tttagcatgt tttccttttt tcataatagt ttatttagtc
     1501 tcctttcttt tttacagaaa gaataaagct aaaatagctt gtatcttgat aacatttatt
     1561 ttaatcttat agtaatggat ttactactta catttttgat agaatattta aataaaatca
     1621 ttcacgccaa ttcaaatatc atgtataatg aataaagtta cgaacttgat agtaaggagg
     1681 agttgttttg tcaggaaaca aacaacaccc aagtaaagat aaaaaagaag ccagtaaggc
     1741 agctacagca gatataatcg acaagcggag aaatctgcgt aaaaaagaag ataagattgt
     1801 gaggaagatc attttagtga ttgctcttac attattgatt attggcggat ttcttggatt
     1861 taccgtgtat cgttatgtag atagcgggtt gaagccttta gataaatcag atgaccaatt
     1921 agtccaagtc gagatcccga gtggctcttc taacaagcag attggtgaga ttttggagaa
     1981 ggacaatatt attaaaagcg ggattgtttt caattattat acaaaattta aaaatttaac
     2041 tggctttcaa gctggctatt atcagttagc gcctaatatg actcttgacg agattggcaa
     2101 acagttgcaa gaaggcggta cttctgagcc aacaaaagtt gcagatggga aaattgccat
     2161 tccagaaggg tacgatatcg atcagattgc tgaacgggta gcgaaggtaa ctggtaaaga
     2221 caagaaagaa tttttagatt tagtgaatga tgagactttc tttaacgagt tgcaccaaaa
     2281 attcccagaa ctgttggaaa gtgcatctaa agctgagaac acaaaatacc gtttggaagg
     2341 ttacttattc ccagccacat atgattatta cacgagtact tcgcttaagg atctagtgat
     2401 tgaaatggtg aacaagacga atacggtcat gcaaaactat tattcagcta tcaagcagaa
     2461 aaatttgact gtacaagaag ttttaactct agcttctcta gttgaaaaag aaggcgtaaa
     2521 agaaaatgat cgtaaaaaca ttgcacaagt cttctttaac cgaatcaaag caaatatgcc
     2581 acttcagtca gatatctctg ttttatacgc attaggtgaa cacaaagaat tagtgacgta
     2641 tgaagataca gctgtagatt caccatataa tttgtatacc aataccggct atggacctgg
     2701 accttttgac aatccaagcg aagaggcaat caaggcagta ttagaaccag cagaaaatga
     2761 ctattattat ttcgtagcag atacgagcac tggaaatgtt tatttcgcta aaacttatga
     2821 agaacatatg gaattagtgg aaaaatatgt aaataattct tagaaaaaca ggctctagac
     2881 attgatcatt tttagaaaaa catttacatt tgaagaaaag cgtggtaatc ttttttggac
     2941 tatattcctc aaaagattac cttttgcggt attttatcga aaaaaggaga cgttacacat
     3001 ggtagagaaa gtttatccaa tgactctaga aggtaaagaa aaattagaaa aagaattaga
     3061 agaactaaaa acagtcaaac gcggagaaat cattgaacga atcaaaattg cccgcggttt
     3121 cggagatctt tccgaaaact ctgaatatga atcagcgaaa gatgaacaag catttgttga
     3181 aggaagaatc acaacaattg aaaatatgat tcgttttgct caaatcatcg ataacgacgg
     3241 tgtagatgca gatgaggtat caattggtaa aacagttaca ttcatcgaac taccagatgg
     3301 agaagaagaa gagtacacga ttgtcggaag cgctgaagca gatccgtttt ctggcaaaat
     3361 ttccaatgat tctcctattg cgcaagcatt gattggcaaa cgtaaaaatg atgaagtaac
     3421 tattgctact ccaggtggcg atatgttagt taaaatcgtc aaagtagaac aagcgtagtc
     3481 tgactgaaag atctagagag ttcatcttac tgatgataca ctctagatct ttttctcttt
     3541 gtgtatttta gatttcaaca aacgatctta catctaaagt cctatagcca acagttaaaa
     3601 aaataggtat gatgaaatga gaatgtgtca ggagggagaa agagcgatga atagttcttg
     3661 gcgttttttt gtggtagttg aagcactact gctgttattt gctgtttggc aaatcgtaaa
     3721 caatactgga cttttgcttt tagtgatatt cggtattttc aatatatatc tagctatgcg
     3781 caaacatcct cgtactaaat ttcaaaattt tcaattgata ttaggaagtt tggtcatttt
     3841 ttttagctta gtcaacagtc ctgctttatg gatgatggct gtatttgccg ttttatttat
     3901 tggtctgaaa ggtgtagaga tttcagggat agatttaacc aaaaatgctt tttggcgaaa
     3961 gaaacagatc atgatgatcc aaacagagca attaaaaaca cataataatg agcggaaaag
     4021 acagcaatta ttcggtaacc aacgtattgg aaacgacgtc tatgaatggg acgatatcaa
     4081 catcgccgtc atttctggtg atactatcat agatttgggc aatacgcttt tgccaaaaga
     4141 tgataatatt gtgattgtaa gaaaagggat aggcagaaca cggatcttag tacctttggg
     4201 cgtagccatc cgattagagc atgcgacatt agtaggaaat gtcttgtttg aagaagagca
     4261 attttctttg aaaaatgaac aaatcaaaat ctacagcagt gattatgatg aaaaccctcg
     4321 tagattgaag attattacta ataccttgat tggagatgtc gaggtgatac gaatatgatg
     4381 ggaaaaatat ccagagcgat gctagctgtt tattcgggga ttgcggcttt ccttattatt
     4441 ctattttcac ttttcactta tttttatgcc agcaatcaaa gtcattggtg gggagaatta
     4501 ctgcgtgcac gtttattata tgttccgctt atcttccatt tgttagccat ttccttaggt
     4561 gtaggattaa ttgtctttct attattatca cttattcaaa agacaaaata cgggaaaatc
     4621 gaagaaaagc tgcgtgcact ttcttctggc aattatgaat ccaaattgtt gcttcttccg
     4681 atcccaagtg catcggacga tttatacatc aaagatattg ataaggaaat cactaagata
     4741 aaagaaaaaa tgattgaagt atctagtgaa ttacaaattg taacgagccg tccccaatat
     4801 gtagatggac aaaccaaaga agaaatttta gaattagaaa gacatcgatt agcccgtgag
     4861 ctgcatgatt cagtttcgca acaattgttt gcagcaatga tgatgatgtc ggcattgacg
     4921 gagcaagcag aaaaaagcga gacaccagaa atgtttcgca agcagttgaa aatggtagca
     4981 gagatcatca acgcttccca gtctgagatg cgtgcgctgc ttcttcatct acgtccagtc
     5041 aatttagaag agaaaagttt aaagcaaggt atcgagcagt tattgaagga attgcaaaat
     5101 aaaatccaga tttcgctgaa atgggatgta gaagatgtaa aactatctag ttccattgag
     5161 gatcacttgt tccgaatcgt tcaagaattg ttatcaaata cattaagaca tgctaaagcc
     5221 aatgaattag aggtatattt gcacaaaata gacaataatc ttttgttacg tattattgac
     5281 gacggaacag ggtttaatat gaatgaaacg aaaacgggaa gttatggatt gaacaatatc
     5341 aaagaacgag tagctggtat tggtggaaca gtaaaaatca ttagttttaa aggtcaaggt
     5401 acaagtgtag aaatcaaggt ccctttgatg aaggaggcat agataaggat gataaaagtt
     5461 ttattagtag atgaccatga aatggtgcgc ttaggcgtct ctttttattt atctattcaa
     5521 gaagacattg aagtaattgg agaagctgaa aatggacgac aaggctatga gaaggcgatg
     5581 acacttcgac cggacgtcat tttaatggac ttagtcatgg aagaaatgga tggtatcgaa
     5641 tcaacaaaag caatcttaaa agattggcca gaagctaaaa tcattatcgt aaccagtttt
     5701 attgatgacg aaaaagttta tcctgccatt gaagcaggtg cagcagggta tttgttgaag
     5761 acctctaccg cacatgaaat tgctgatgct ataagggcaa cgcaacgtgg cgagcgagta
     5821 ttagaaccag aagtgacaac aaaaatgatg gaaaaaatga gtcggcgaaa tgagcctgta
     5881 ttacatgaag aactaacgaa tcgggaaaat gaaattttaa tgctgatttc tgaaggaaaa
     5941 agtaatcagg aaatagcgga cgaattgttt attacactga aaacagtaaa aacacatgtt
     6001 tccaatatat tggctaagct agaagtggaa gaccgtacac aagccgctat ctatgcattc
     6061 aaacatggtt tggtgaaata aaaacgatga taaagaggat aatcaaaagc tgagaaaata
     6121 actattattt ttcttggctt ttttacaata atgatgcttg cttttgtttt tgtaaaggct
     6181 ttgttatact gagaaaagaa gatactaaag gaggaaaacg gtgaaacaaa attttgcaat
     6241 tgtaggtctt ggacgatttg gcggaagtat ctgccgaaca ctgatcgaag ctggtcaaga
     6301 agtactggct attgatagta atgaagatcg ggtcaatgaa tatatgaata tagccactca
     6361 tgctgttgtg gcaaatgcac aggatgaaat gacacttcga tcattaggca taagaaattt
     6421 cgatcatgta attgtcgcta ttggagaaga tattcaagcc agtatcttag ttactctgat
     6481 ggtaaaggaa atgggcgtac caaatatttt agcaaaagcc caaaatgagt atcatgccag
     6541 agtacttgaa aagatcggag ctgatcgcgt cgtccatccc gagcgagaca tgggaataag
     6601 gattgcgcat aatttagtgt caaagaatat tttagattac ctagagttgt cagataaata
     6661 ttctttagca gaaatccgag tctccaatcc taaatttttc aacaaaacat tagctgaatt
     6721 aaatttcagg caacgttttg acttaacagt agtcgccata cgaagagtag atcaaacggt
     6781 tatcgcttct cctgcagcag atgaatatgt tcgtgaaaat gataatctac tggttattgg
     6841 agagacaggc gatgtggata ttctagacga taagatgaat caatagaaag aaaagagaaa
     6901 gtaggttgtt gggatgaaat taacggtaac gccaaaagca caaaagtggt ttgactcaga
     6961 aattgatctg ccagaaactt acggaatacg tttttttgga aaagtctacg gcaaaacaga
     7021 ggttcatgat ggtttttcga ttggaatgtc agttgaacag ccagaacatc ctgtaaagaa
     7081 agtggaaatt gatgggacac ttttttttat cgaggaagca gatgaatggt tctttaaagg
     7141 atatgattta cttgttgatt atgatcccaa cctggaggag ccaacctatc actttaaaaa
     7201 acaataaaaa aagagagggt gacacaattt ttgtcacctt ctcttttttc ttttgaaaat
     7261 attgttttca gctattaata tcctgcagac aattttactt gattggtttg aagctcatgg
     7321 gtggcaaagt agcttttcag attggttaaa aaaatagcca ttaatttctt ttgaaaatca
     7381 ggtgtcaaac ctgcaatgtg ggaagtaatc agtacattgt ccattttcca aagaggatga
     7441 tcttgtggaa gaggctcttc ctcaaaaacg tctaatgctg caaaggaaag ttgctggtta
     7501 tttaaagcct gcaccagatc ttgtgtctta acagaagcac cacggccaac attgatgaat
     7561 acagattttg gatcaaagtg ttcaaacata tgctgatcaa agagcccagt tgtttcctcc
     7621 gttagaggta agatattgac tacgaaatct acttcaggaa gaacttcctt tagttgattt
     7681 aaagaaaaag tcttttccat tccttctgct ggatgaccgg atgtattgat tccatagcag
     7741 ttcatgtcta atgctttgca tttttttgca atcatttttc caatatggcc agttccaatg
     7801 attagtattt ttttcccttg gatagattgg taatggaccg atgaacctaa ccatttcatg
     7861 tcactctgtg ccttttgtgc ttgaaaaaga ccacgagaat acccaaggat gattcctaag
     7921 acatgctccg taatcgattc actgtggatg ccactaccat ttgaaagtaa aatatgtttc
     7981 tttgaaaaag tctcaagggg tagctgatca acaccagctg agatagattg gacccatttc
     8041 aaggtagaag aggctaatag tttttcttgg tagtctgagt tccagcccaa actgatctct
     8101 acattttgta gatcctctgg ttgtaattct gttttaaaga tataaccagg cgctatagta
     8161 tggatttctt cgataaattc atcacgaaat ggacgatctg cataaatgat tctttctgac
     8221 atagttaatt tcccgccttt ctttactcta agtgtagcgc atacaagaag aactttgaag
     8281 cgccttgctt gttttttgag aaagaaatat ttttcaaata aaaaaggggc tgtgacaaaa
     8341 aaatgtcaca acctctaaaa gggtctattg aagtagaaat aacgagttgt ctttatttct
     8401 taattgatcg atatttttgc ccatcctttg acagcttgag caaaaataaa gattcctgca
     8461 aaagcaatga aggcgctagt gtcaaaagaa ggaagaaaga ccatcaagat tgttgcaatt
     8521 atagtaggca atgaaattga atagatcaat gtttttaaac aatcaagaaa agtagctttt
     8581 gttagtcgaa agcgtgcata gagaaaagca gcaatgttgg taaatagcaa agtgatcagc
     8641 agattcagaa aactaggata aatagaaaat ataaatgtca ggacttttat ccaaagtggt
     8701 atagatgctt cacttaaaaa agaacggagc tgcttacctg ttaagttttt taaagattca
     8761 ttggtgtagg gaagttctaa ttggttattg ttcaacaaag cagaagtcgt gcctgtatta
     8821 ggaagagcta tgataagttt gttttttaac atcccgacac taacaaaatt ccccatcaaa
     8881 tcagtagaga tgtcctgttc tgttcgtttt ccctctggat caaatgtgaa gataatggag
     8941 tttgtttggt aaatgaatcc ttctttttct tcggtttgaa gctgcccgtt ttggattgta
     9001 aaatcaggaa tgcgagtagc aatcttttgc ccatccaatt gaatgttgtt taagacttga
     9061 aaaacttgaa aagagattgg caaagccata attatactca aaactaaaag atagacaatg
     9121 gctttccaaa atgggacatt tctcgcctcc tttaggtctt tgaatcgtat aaaggaactg
     9181 ataattaatt gttttgttgt cataagtact cctcatctag tagtgtcatc acttgcaatt
     9241 ctagcgaagt atcaagtgaa atacaagtgc tttaaggaaa cagctgaatg attggtgaaa
     9301 tatcaaaaaa atgttattct tgtctaaaga ctatccaata taaataaaat ggaggaagga
     9361 atagtgccta cacagccaca gagatcatca aaaaaactat ttggagtagt ggttttgaat
     9421 gcggtgatca ctttatcaga atttattgga gggattatgt cagggagttt gtctcttgtt
     9481 tcagatgctt tccataacct tagtgataca ttggcaatta ttttttctta cggggcacaa
     9541 aaaatggccc gaaaagaagc aaataaaaaa cgtacgtatg gctaccagcg tttagaaata
     9601 ttgagtgctt tcatcaactc attcatattg atcatattat ccttgttttt aaccgttgaa
     9661 gcattcaagc ggttcacttc accagaaaag ataaatagtc atttgatgct aacagtagca
     9721 gttattggat tgctcgcgaa tttgttctca gtattattat tacgacaaga agcagatgaa
     9781 agtttgaata taaaatctag ttatttgcat ttgttaagtg atacactgtc ttcggtaagt
     9841 gtgattatcg gagcaatttt gatccgtttt ttcgatatct actggatcga tccagttatt
     9901 acactgatca tttcgatata tatattgata gaagcgataa tggtgatcaa aaaagctgcc
     9961 gctattttga tacaaagtgc acctgctatc gactatgaaa aaatggaaca agaaattgaa
    10021 gcaatcgatg gggtaaagga tgttcatcat gtccatattt ggcagtatag cgaaaagata
    10081 attattttcg acggacatat tgattttgaa gatcagctac taagcgagat tgaaaagata
    10141 tatcctcgta taatttcttt attaaaatta aaatatggta ttacacatgt gacgatccaa
    10201 gcagagacaa aggcaaagga tcaaaaaaaa ttaatttttt taaataaaga tgaaagttat
    10261 tgaaatatag agtatgataa ataagaattt ttaataaggt aggttggctc atgaagcagt
    10321 atcgacagtt aaaaggatat ttagaagcaa aaggatattg ggagatacta gtctatctct
    10381 tttttggtgg tttggctacg attgtaaatt ttgttagttt tgctctcgca cgccaatatt
    10441 ttgatttaag tatggcctta tccaattcta tttcgtggtt ttgttctgta ttgtttgctt
    10501 ttgtaacgaa caaactttgg gtattccatt caaaatcacc gaattttaca catgcactta
    10561 ttgaatgcgg taagttcttc ttctaccgta tcctttctta cggattggac atgggggcaa
    10621 tggtattgtt gatcaatgtt atgaacagca atgagtatgt ggcgaaaatc atcactcaaa
    10681 ttattgttat ccttgcgaac tatattttca gcaagttgtt tatttttaaa gaaactgagg
    10741 tttttgaaga agaagtaggt caaagtgaaa aagacgacta ggcataacaa ttgaagaatt
    10801 tccttctctt tgttactctt tgtaatgtag acattattaa gaaaacattg aggaggaatt
    10861 tttttatgac ttacacatta ccagatctac cttatgcata tgacgcattg gaaccttata
    10921 ttgacgaaga aacgatgcat ctgcatcatg ataagcatca caatacttat gtgacgaatt
    10981 taaattcagc aattgagaaa tacccagaat taggcgaaaa aacaatagaa gaattattat
    11041 ctgatatgga cgctattcca acagatatca agacagctgt acgtaacaat ggtggcggac
    11101 atgctaacca ttcatttttc tgggaaatca tggcaccaaa tgctggtggc gaacctacag
    11161 gagaaataaa agaagcgatt aatgaagctt ttggtgattt ttcttctttt aaagaagaat
    11221 tcaaaaaagc agccgctgga cgatttggtt ctggatgggc ttggcttgta atggaaaatg
    11281 gaaaattagc tattacctct actgcaaatc aagattctcc attgatggaa ggaaagacac
    11341 caattctagg tttggacgtt tgggaacatg cttattattt gaaatataaa aatgtccgac
    11401 cagattatat tgcagcattc tggaatgtta ttaattggga tgaagtaaac aaacgtttcg
    11461 aagcagcaaa ataaccattt cctccgtcat atcagtctta aaaataattt ggaaaataaa
    11521 agagcgaaag cttgctagcg ttttctaaat tgggtataat gttcttgcaa ggttacttgc
    11581 aagaactttt ttggaggata tacatttatg gaacacggaa cggttaaatg gttcaacaat
    11641 gaaaaaggat ttgggtttat tacagtagac ggcggagatg acgtatttgt tcatttctca
    11701 gcaattcaag gagacggctt caagagccta gaagaaggtc aagaagtaga attcacgatt
    11761 gttgaaggtg cacgcggacc tcaagctgct gaagtgacta aattataata atttgttgaa
    11821 taaagacacg acttcttaga gagtcgtgtc tttttgtcta tttatgaagt ttttagattt
    11881 tttggctaag taaatgcatt tgttcacttg acatgtccta actaattatc aagcaaggaa
    11941 cattttcttc gttagtaatg gattttaaag aaatttcagt ttttggatgt tgaataaaag
    12001 accatttgtg aaaccactag tatacatact cattaccatg tcaattctat tactttaaaa
    12061 gaaaatcgtt ttactttgaa gaggagcata tctatccatt aaaagatgat ggtaatgcaa
    12121 tcactagttc gagtcatttt aagagccatg cagtgagctg gaaataaaac gaatagaaga
    12181 tgggtttaac aaaaggacca gtcataggaa gaaattgtcg tgaaatcgaa tgataatctg
    12241 aacggtaggc aaagactgtt aaaaagatgg gaaaggtatg tgcgatcatt ttactgcacg
    12301 attatttgga aaataccttg ttgttcgctt tttctggaaa aaatacataa aaaaagataa
    12361 aacgattgcg aactgcgtgt attcggtctt tcgatctttt tcacggtatg gtatgatagt
    12421 taagaatttt gagaaatttt aaaaaataat aatcgttaca caaaaatgtt ttttaaagtt
    12481 ttcttatgat taaaaagtga atttgctgat tgaacagtta tatgagacaa tattttagaa
    12541 aatgtgaaac agaggtgtat tatgaagcga tggcaaaaag ttctattaac cttgttaggg
    12601 gtattcgttt tgctgattgc cggattatca gcatacggaa tcagatttat gggagaagcg
    12661 agccaaacag taaacaaaat ttctaaaaac tctaatcgaa tatcctccaa acgttctgag
    12721 agagtaagca ttgatgacaa agaacctttt tcggttttgc tattaggttt agataccggt
    12781 ggattaggaa gaacggaaca aggacgttct gatacgatga tggtagtaac agttaatccg
    12841 aaacaaaaaa aatcaacgat catcagtttg gaccgagata tttatacgaa tatcgtaggt
    12901 tatggaacgg tagataaatt aaatcatgcc tacgcatttg gtggtgtcga aatggccatg
    12961 gattcaattg agcaactgct cgatattcct attgatcatt atgtaacgat caatttggat
    13021 ggcatggaag atttgatcaa tgcggtcggt ggcatcaaag tgaataataa aattgacttt
    13081 actttggatg gtgtccatgt accagtaggt gaacaaacat tgaatggaga aaaaggttta
    13141 gcctattctc gaatgcgaca cgaagatcca gaaggcgata tcggtcgaca acgtcgtcag
    13201 cgggaagtag tgacgaaaat cgtcaataaa gttttaagtt tagatggcgt aagcaattat
    13261 agaaaaattt taaaagcagt tgagaacaat gtgacgacgg acttagattg ggatgacatg
    13321 cttgatgtag cgacgaacta caccccggcg tttgaaacaa tcaaacaaga ccagttgcaa
    13381 ggcgaaggac aaatgatcaa tgatatctac tatcaaattt taggaaaaaa tgatcttctt
    13441 tcgatccaaa atgaactgaa gaaacaatta aatataaaaa caagcgatac attacctaat
    13501 ttgaaaaatg agaatgcttc aatgatgttc tttgatgatt cggaagatgg cgattcgaca
    13561 aacggtgatg gatcgaatag tgaagggtac accgattatt cttcctattc agaaaacaca
    13621 tatgatcagt accaagatcc gaatacctat tatcagcaaa cagaagatac aagtcagtac
    13681 tatacaccaa gtcaagatac tcaaagttat gatccttacg ctggacagga acaaaatggt
    13741 tatggttatg accagaataa tgagtatgga acaacaagtg ggtactagaa aaaaatagtt
    13801 tggttaataa agagtgccac ctaaattaga caaaaaa
//
LOCUS       DANKHL010000065        13809 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000065 DANKHL010000000
VERSION     DANKHL010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.068"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="PW101_001358"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="PW101_001358"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDL2513763.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="PW101_001359"
     CDS             complement(2262..2513)
                     /locus_tag="PW101_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HDL2513764.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="PW101_001360"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="PW101_001360"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDL2513765.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="PW101_001361"
     CDS             3463..3681
                     /locus_tag="PW101_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513766.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3970..4482)
                     /locus_tag="PW101_001362"
     CDS             complement(3970..4482)
                     /locus_tag="PW101_001362"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDL2513767.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="PW101_001363"
     CDS             complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="PW101_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDL2513768.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6935..7378)
                     /locus_tag="PW101_001364"
     CDS             complement(6935..7378)
                     /locus_tag="PW101_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDL2513769.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7379..8167)
                     /locus_tag="PW101_001365"
     CDS             complement(7379..8167)
                     /locus_tag="PW101_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2513770.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8164..9105)
                     /locus_tag="PW101_001366"
     CDS             complement(8164..9105)
                     /locus_tag="PW101_001366"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDL2513771.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9282..9869)
                     /locus_tag="PW101_001367"
     CDS             complement(9282..9869)
                     /locus_tag="PW101_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HDL2513772.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10090..11397)
                     /gene="obgE"
                     /locus_tag="PW101_001368"
     CDS             complement(10090..11397)
                     /gene="obgE"
                     /locus_tag="PW101_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDL2513773.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11691..12701
                     /gene="gap"
                     /locus_tag="PW101_001369"
     CDS             11691..12701
                     /gene="gap"
                     /locus_tag="PW101_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL2513774.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12786..13721)
                     /locus_tag="PW101_001370"
     CDS             complement(12786..13721)
                     /locus_tag="PW101_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2513775.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3096 c         2435 g         4470 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta
     3721 aacttttacg ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc
     3781 taaagccagt ttttcggtat taagtcaaat tgcggaaaaa gtgatttttt aaacttctac
     3841 tggtctttgc ttgtttccat ttataaaaaa atagcgaccc tcaaaagtca gatagagaat
     3901 ctgacttttg agggtcgctt taaaatcgct tctttaagaa taacatcaag aatagcttct
     3961 tttttctgct taatattcca ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc
     4021 atggagatcc atcaattcga tcaaaaatgc gcagcctacg actacaccgc ctaattgttc
     4081 gatcagttcg atcgtcgctt tgattgttcc tccagtagct agcaaatcgt cacaaatcag
     4141 cacacgttga cctggtttaa tggcatcctt atgcaatgtc aatgtatctg ttccatattc
     4201 taatccataa gtcacttcga tggtttctcg aggtaacttc ccttttttgc ggactggggc
     4261 aaaaccgact cctaactcat aagcaacagg acatcctacg atgaatccac gggcttctgg
     4321 cccaactacc atatcaattc gtttttcttt tgcataattt acgatttgtt tagtagcttc
     4381 ccgataagcg tcaccatctg ccatcagtgg ggaaatatct cgaaacacaa ttccttttga
     4441 cggatagtcg ggaatactgg caatataatc ttttaaatcc actctttgtt tcctcctagg
     4501 ttgttaacca tttctttatg gtaggaatat cgctcaaaag caaaaattcc tcaactttga
     4561 ttttattgag ccgtcgctgg tataatctgc tctcagtcaa aggatgactt tctgggctag
     4621 cgactttttg taaaacaccg ttctctattg taacaaattt caattcaaaa aacacctgta
     4681 tcataaaaat taataatttt tcagggatac gcaaatattg agctattgct tttattttat
     4741 gacgtatatc aattcgttct tgagaatgga tcaatttaaa caattttgcg tactgttctc
     4801 ttgtgcctac cccattcaaa taggcctcat caggtgaaat ccccatcaga taaacacgat
     4861 tgaatgaata agaccgaaaa acttctttca gatccgctaa atcatctgga caatccataa
     4921 caactagaga gtttggtttt cttttttcaa ttattgcctt tacagaatct agtccttcga
     4981 aaacaatcgc agcttcctgg ataatctcag gcacgaattt caacgtcgaa gaatcaaatg
     5041 ctaaataaag gctaccttcc gacgttcggc tctgttcacg gaaacgtttt gcccgccaat
     5101 caaatacttg cagaccaccc acagaaaaat cagataccat cagctgcggt ttcttacgcc
     5161 cgttccattc attgatcgaa agctttccta ccacatctac ctgattattc agcagttctt
     5221 cttcttgagt accaaaacca aaagcgaccg catcaagctg actcgatgca tcggaaagga
     5281 caaatttcag atgctgttgg tttgctccga ttcttttgat attttctgct tggacttgtc
     5341 gaaataaaaa ctgaggcaaa ggattatccg taccaaatgg cgccaatagt ttcagctcat
     5401 caattcgctc tactgttact tcatttggca gcaatacttc atcaattctc aactcaggcc
     5461 ctctcatcaa gtcaatttga tgatcgacta tatattggtt catcttctcc tgtagtatag
     5521 tgacattttc acttggcatc gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa
     5581 ataaatctcg catctggtca agcatttcaa aaagattcaa ggcttcaata ctacgtccag
     5641 atcctttagc tgatccatct tcttttaaag taaggacaag cgttggttta cccgtttcat
     5701 tcattatttt cccagcaaca atgcccaaaa cgccttcatg ccatcctcgg tttaccaata
     5761 gatggatatg attctcctta ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga
     5821 ttgcttttct ttcttcattg atctcattca atttttttgc ttgaacggat gcttcctctt
     5881 catcaaacgt cgcaagtaac gagacggcag gacgtggatc gcccatccga ccgattgcat
     5941 ttagtctggg agcaatcgaa aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc
     6001 cgctttcttc aaatagcgcc tgaagtccta tccgttctga atgatgaatg gcatcgattc
     6061 ctaatgaaac aaggattcga ttctcatcag tcaatgaaac taaatcggcg atcgttccta
     6121 tcgctactaa atccaaaaat tctgcaggcg gttcttccaa taatgcagaa gctactttga
     6181 atgctactcc gactcctgcc aaatctggaa atggatagtg ccccgctgga tgacgagggt
     6241 gaacaatcgc ataagcgtct ggtaaattct caggtagttc gtgatgatct gtaatgatca
     6301 cgtctacacc agcttgctga gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa
     6361 cagtgatgat caactggatt ccttcttcga tcttttcttg gtataccgat tggtttggtc
     6421 catacccatg ctcaaatcga tttggcagga atacttctac ttctgcacca agtaattcca
     6481 gtgtttcttt cattaccgtt gcactggtga tcccatctgc gtcataatca ccgtaaacga
     6541 ggattttttc tccactgatt acagcttctt ggattcggct gattgcttta tccatatcgt
     6601 gtatcagata cggatcatgt aattcttgtt cctttggatg taaaaatcgg ttgatatcct
     6661 tttcctcatg gtaacctctt tgccacagta acttaccgac aaatggagat aatttcagtt
     6721 cacggatcat ttggagaaag gattcagaag gctctgtctc gcttaacttc caatcaaaat
     6781 ttgcttgtct cactttctca ctcctaactt agttattata gcaaaggaaa tcagcaaaaa
     6841 aaagcagaaa gaaagaataa aaacagatca gatccgctag acagatgttc agcaggacct
     6901 gatcttttgt acgaccattt atgaaatatc cctattagtc aaaataattg aagctttgtt
     6961 tgggcgattg agttttttgt tcttccggcg ctttcaattg actgatttgt gcggttttcg
     7021 cttgtaactc cgcctgataa gcagcttcca actccgcacg ttcgtttcta aactcgcgtt
     7081 gctgttcttc ttttgcttct tcaattttct tttgaagttc tgtttgatat tctgacattt
     7141 cctgagtcag ctgtttgatt tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa
     7201 atacaatcag tgcaccaatc agcgcagaac cgatgatcac taagatcaat ggcgcagaaa
     7261 aggaagtgaa gccaaaactt acaggaacat ttttattgtt caatacagca aaaagaacaa
     7321 taatcaaaac taaaacaaat ccaagaatca cattgctttg ctttttcaat tccttcacct
     7381 actttttgtt aaaaattccg ctggctaaat aatcaccaat cgttggaaac agcgtataaa
     7441 attttgatgc aacttctaaa ataaacggtt gattgatttc ccgttttgga tgtctcatat
     7501 tgcggacgat catttttgcc aacttggcag gttctaaaac gatttgtccg actttttcca
     7561 aatagcttcc gcttggatca gccttatcga agaaatttgt ctcgattgga cctggattca
     7621 ctgtcgtaac agctacgcca agtggtttca attctaatcg caaagcgttg gagaagccaa
     7681 ggactgcaaa tttcgtggca gaataaaccg ttgattttgc agttgccatt tttccagcca
     7741 tagaggcgat attaatgata tgaccttgtc ttctttctgc catatcgatc gcaaattttt
     7801 gtgtcaaaac catcattcct aagatgttga cttcaaacat atcgtatgct tttccaaggt
     7861 cgaatgtaag gaagtcttcg aaaatcccaa aacccgcatt gtttaccaga atatcgacgc
     7921 gcccgacttc ttccatcacc ttttcatata attcgtccac gctttcagga ttgctgacgt
     7981 ctaccttgaa agcataagcc tctttgccgc tcaagcgtct gcactcgtct ctgacacctt
     8041 ctacaaatgc cattcttcgt gcgcaactta taacgattgc tccttgcttt gctgcttcat
     8101 aacagatttg tgcgcccaga ccgcctgaac tacctgttac taaaacaact ttatcttcta
     8161 aattcatgcc tcttcctcct cgcgaaatgg aatctcaatg ctatcaaaat ctttcataat
     8221 cgtcgtgttt tcaaacactt cttgtgcttc tttttcaagc tctaaagctg cttttcctaa
     8281 atatctcgca ctgatatgag taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc
     8341 agcagcttga tgagtcgtgg aatggaaata gttgtgcgcc attcttgctt catctttatt
     8401 aaatgtgctt tcatgtacca gaatatcgct attttccgca agcacaacgg aattatgtgt
     8461 ttttctcgta tcgcctaaaa tagtcacgat tcgtcctttt ttatctggac ctacgaaatc
     8521 ttttccattg atcgtccgcc catcttctag ttggatcgtt tcaccttgct tcagtttgcc
     8581 gtacagtggt ccagctggaa tacctaattc ttttagacga tccacctgca attctccttt
     8641 atgatcatgt tcaaccacac gataaccaaa actgtcgatc ccatgattta gtttttttgt
     8701 ataaacagaa aattgctgat cagtaaaaat cggggctgtt tcatttagct caatgaattt
     8761 cagcggataa cttaaacgcg tttgcgaaat acctaaagag acttttatgt attcctctat
     8821 cccttttggc ccgtaaatct ctaatggcgt atccccacct tgaaatgacc ggctgctgat
     8881 gagcccggga agtccaaaaa tatgatcgcc gtgtaaatgt gtaatgaaaa ttttaccgat
     8941 ttttctcgga cgaatagtcg ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa
     9001 caaccaaact tcattgcgct cgtctaaaag tttcaatgct atacttgtca catttctatg
     9061 ttttgcagga acacctgcac ctgttcctaa aaattgtaat tccataatct acctgacttt
     9121 ctttaagttc ctctttaatt ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa
     9181 tgggttcaaa ttaaaaataa cgttctaaag cgatagcgtt ttctttgaaa attactataa
     9241 aaacagcagc gaacccgttt gagatcctct gctgtttttt attaatttga agatccagaa
     9301 ctttcattgt cgttggaagt ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct
     9361 ttcgcatgat tttcagcttc tttcgcatca tgcacatctt tttcgacatc ttcataatcc
     9421 aagcgagtaa tttctccgcc taattccatc attaccaatt gattcagtgg gcgattgtcc
     9481 tcttcatcag caatcaagat cagtcctgta gctccttcac cgattttatt tcctacaaag
     9541 tcgaacacgg tctgggcatc ttttatttct ttggcatctt gtgtcgcacc gatcattcct
     9601 ccggcaaacc atcctaataa aatccctagc ggaccaccta atacaccaac caacattccg
     9661 attaatccac ccgtagaaga tttgttatta ccggtaaagt cgataaaatc ttctattttg
     9721 aattgatgtg tcccattagg atcatggctg acaactgcca tctgttctcc tttaatcgaa
     9781 cgaacagcat gtaacttttt gatttctgaa aatgcctgat atgcctggct ttctacttga
     9841 aaatttaaaa tgattactcg tttgctcatt aaaaacgccc ctttcatacc ttcttgttat
     9901 ttattttacc tttaattgaa aaataagaaa agaaaagcac tctttttttc caataaaata
     9961 gggctgtgac aaaagtcgta tcacagctcc tttttccgaa taaacggcgt tcttgaagct
    10021 gatcacttct gtcacaacct cccttaaatc tgaatctata ttttttggta tttgttttta
    10081 tttgcttgcc tattcgacaa attcaaattc aaactcaccg atacgtacaa ggtcgccatc
    10141 tttcgcaccg cgtgcccgaa gagcttcatc cacacccata ccacgtaatt gacgagcaaa
    10201 acgcatcact gtttcatcat gatcgaaatt cgtcatttgg aataattttt cgatcttctc
    10261 accagataag atccacgttg catctgaatc acgatcgatt tggaactcag gaccttctgg
    10321 attgaatcca tagtgtacag tttcttcttc cagttcttct tcgtacaatg ggaattctgg
    10381 cgttacttct aacaaatctg ctgtagcatt caataacgcg tccaatcctt gtcgagtcac
    10441 tccagaaatt ggaaatactg ggatgtcatc agcaaattca tcttcttttt ccttgttcag
    10501 ctgttctttg aatttcacta gattttccgg tgcatccggc atatccattt tgtttgccac
    10561 gatgatttgc ggacgctcta gcaagcgtaa gttataagta gataattctt tgttgatggc
    10621 aagatagtct tcataaggat ctcgtccttc catcccactc atatcgatca catgtaaaat
    10681 tactcgtgtt ctttcgatat gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc
    10741 tccttcaatc aagcctggca agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac
    10801 cattcctaga ttaggtacga gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga
    10861 tgaaataacg gatagtaaag tcgatttccc gacagatgga aaacctacaa gtccaacgtc
    10921 tgccaatact ttcaattcta gttcgatttt tcgctcttgt cctggttcac cgttttcagc
    10981 aatttccggt gcaggattcc gaggagaggc aaaacggata ttccctcgtc caccgcgtcc
    11041 accttttgcc acaactagtg tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt
    11101 ctccgcatct cggacagtgg ttccttgtgg tactttaaca taggtatctt ctgaaccacg
    11161 accgtgcatc cctttactca tcccattttc acctggctgc gctttgaaat ggcgattgaa
    11221 ccgaaaatcc atcaatgtac gtaacccttc atcgacgatc aagatgacat cgccgccacg
    11281 accaccgtct ccgccagccg gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac
    11341 cattccgtct ccgcccttgc cagctttcac atcgatcgtt acttgatcta aaaacatgga
    11401 cataattaag tcctcctatt attcatttat ttatttgact gcctgaattt ccacaataaa
    11461 caaaagtctt acttatttta tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga
    11521 ttgtttacct tcttcttttt ttatcatttg gtaaggtttc caaccttttt tctgctacca
    11581 agtataattt tttgacaaaa tattctttgc gtagcagaat aacagtgtat taattgtatt
    11641 aattgaggat gccgtttcac agcaccccta ataaaggagg aacttttgat atgacagtaa
    11701 aagtaggtat taacggattt gggcgtattg gccgtctggc tttccgccgc atcaaagaag
    11761 tctcagatga tattgaagta gtagcaatca acgacttaac aagtccaact atgttagcac
    11821 aattattgca atttgactct actcatggta cttatcctgg cacagttact gcaacagaag
    11881 actcaatcgt agtggatggc gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa
    11941 tcccttgggc aaaagaaaac ggtgtagata tcgtactaga atgtactggt ttttatacat
    12001 ctgaagaaaa agcaaaagcg catttagatg ctggtgtcaa acgagtagtt atttctgccc
    12061 cagctggtgc tatgaaaacc atcgtctata acgtcaatga tgatacatta gatgaaaacg
    12121 acaaaatcat ctcagcaggt tcatgtacaa caaactgttt agcacctatg gcttatttct
    12181 taaataatga atttgggatc gaagttggta caatgacaac agttcatgcc tacacttcca
    12241 cacaaatgct cttagatgga cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg
    12301 ctgacaatac tatcccacat tctactggtg ctgcgaaagc aatcggctta gttatccctg
    12361 agctacaagg aaaattacaa ggacacgcac aacgtgttcc agtcgttgat ggttcattaa
    12421 ctgaattggt ttcagtattg aaaacaaaag taactgctga tcaagtaaac gaagcaatca
    12481 aaaaacacac agttgacaat ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg
    12541 atgtcattgg aacaactcaa ggttcgatct ttgacccaac gcaaacagaa gtaacaactg
    12601 ctggtgattt ccaacttgtt aaaactgttg catggtatga caatgagtat ggatttactt
    12661 gccaaatgat tcgcttacta gaaaaattcg ctaacttgta atttgataga tcaatcgata
    12721 aaagaagcct ttgactggat ccagtcaaag gcttctttct tttcatcccc tacttttaaa
    12781 ctcgttcaat gaacatattg gcttaaaaat tgttggacta cttgctgata tttttcagga
    12841 tctgtcttgt atgcttccgc atgctcggct ccttttacaa catacttttc ttttggtccc
    12901 ttcgttgcac gatagacctt atctaacatt tcaaatggta caaatgtatc ttcgtctcca
    12961 tgtataaaga gcattgggcg ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg
    13021 aaaaagtagc cagcacggac ttttgtcact agactcgtta cttgcataag cggaaatgct
    13081 ggaagactaa acatatcgtc tagctgataa gcaagctcat catgaacgga agaataaccg
    13141 caatcttcaa tgattgcttt cacattatct ggtagttttt ctccgctagt catcataact
    13201 gttgctgctc ccatgctgac accgtataga acgatttctt gcgattttcc attgttttct
    13261 agcactttgt ttatccactg tacatagtct ttacgctctg gccatccaaa tccaatgtaa
    13321 tccccttcgc tttttccatg tcctcttgca tcaggaacaa ggacattgta acccatatca
    13381 tgaaacatct ttgcgtaatt ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca
    13441 agagctgttt tttcactttt cgtttcagca ggaagataaa tcgctgataa tttcagaccg
    13501 tcttttgaag tgatggtcca atcgcttcgg ttttcttttg aactaaacca tttatgtgca
    13561 tccgtttctt tggatgtctg tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca
    13621 gaaggcacaa cagcataatt ataaaaataa ttccctgcaa acactaacgc aacaatcaga
    13681 gctaaagcga cgcctaaaag gattttcatc caaagtttca taaatcatcc atctcatttc
    13741 tcttttttca tataaccttg aattagtata atcaaagaat ctctactatg caatgttcat
    13801 ttttataac
//
LOCUS       DANKHL010000066        13726 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000066 DANKHL010000000
VERSION     DANKHL010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.069"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            129..1556
                     /locus_tag="PW101_001371"
     CDS             129..1556
                     /locus_tag="PW101_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2513776.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1648..2013)
                     /locus_tag="PW101_001372"
     CDS             complement(1648..2013)
                     /locus_tag="PW101_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL2513777.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2010..2375)
                     /locus_tag="PW101_001373"
     CDS             complement(2010..2375)
                     /locus_tag="PW101_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL2513778.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2567..2815)
                     /locus_tag="PW101_001374"
     CDS             complement(2567..2815)
                     /locus_tag="PW101_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513779.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2814..2942
                     /locus_tag="PW101_001375"
     CDS             2814..2942
                     /locus_tag="PW101_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HDL2513780.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2999..4693
                     /locus_tag="PW101_001376"
     CDS             2999..4693
                     /locus_tag="PW101_001376"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDL2513781.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4697..4816
                     /locus_tag="PW101_001377"
                     /pseudo
     CDS             4697..4816
                     /locus_tag="PW101_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4793..5089
                     /locus_tag="PW101_001378"
     CDS             4793..5089
                     /locus_tag="PW101_001378"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513782.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFLTWLDLLPQMQA
                     FEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5467..6600
                     /locus_tag="PW101_001379"
     CDS             5467..6600
                     /locus_tag="PW101_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL2513783.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6631..6951
                     /locus_tag="PW101_001380"
     CDS             6631..6951
                     /locus_tag="PW101_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HDL2513784.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6980..7444)
                     /locus_tag="PW101_001381"
     CDS             complement(6980..7444)
                     /locus_tag="PW101_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2513785.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7575..8828)
                     /locus_tag="PW101_001382"
     CDS             complement(7575..8828)
                     /locus_tag="PW101_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2513786.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8857..9174)
                     /locus_tag="PW101_001383"
     CDS             complement(8857..9174)
                     /locus_tag="PW101_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2513787.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9200..9520)
                     /locus_tag="PW101_001384"
     CDS             complement(9200..9520)
                     /locus_tag="PW101_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2513788.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9984..11399)
                     /locus_tag="PW101_001385"
     CDS             complement(9984..11399)
                     /locus_tag="PW101_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2513789.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11416..12732)
                     /locus_tag="PW101_001386"
     CDS             complement(11416..12732)
                     /locus_tag="PW101_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2513790.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12882..13580)
                     /locus_tag="PW101_001387"
     CDS             complement(12882..13580)
                     /locus_tag="PW101_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDL2513791.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT         4361 a         2787 c         2359 g         4219 t
ORIGIN      
        1 tataacataa aaaacctttt ttaacggcat ttatatgctt tttcagaaat taactgatga
       61 tttcaaaaag aaaacatgtc ataatatttc ccatattgtg caaaaaaata caaggcggag
      121 gctctactat gattgaagaa tatattgaga aaaatatttt aagacagctg tttttatgtg
      181 ggcaattcta tgtaaataaa gaagtcaatc tagaaaagct ctccaatctc ctgcatgttt
      241 gcaaaaccac tttactcaat gatatcaata acataaaaaa agaatttgaa gaacagatcg
      301 cttatacaca tcgggaaaag gaccgttaca ctttatattt ttcagaacat atacctcgat
      361 gcaagatcat gcagcaattg tctcaaaatt cgttgttttt aaaaacttgt ctgctttatt
      421 tggaagaaga tgaacctgat tatcttcaat taactgaatg tgaattcatc tctgtcagca
      481 aagcttattc attaaaaaaa caagtattag cttattttaa tgactgcggg atcgaaatcg
      541 accgctactc ccctcgattc actgaaatgg aaagacgtct gctgcttctt aatgtgtcgt
      601 atcgtttagg cggttttaat tcttgggagc tacctgaatc gttttttgaa cgtgccgatc
      661 gtttcattga atccgtaacg gaaaatagtg gcagatttta tgataaagaa aataaagaaa
      721 tcttatcgat tggttttgct atcagtttcc tacgccagca agtgtgtgct gtgacaatag
      781 attcaaaatt tatcgaagaa atcaaaaaac gtcctattta caattatgtc gaatcagcat
      841 gggaaaacac tgatttccaa acctattata aaaaagaaga attcgccttt attttgacac
      901 tattcaatct ttgtaattat ggtttccatt cttatcagtt gattgcggaa gactttcaac
      961 agctacacca agtgtttatt gacaatactc ccgaaataaa agaactagtt gctacctttg
     1021 aatctcactt caatcaagaa ttattcggaa accaaccgtt cgaacgtgcg ctgattcatc
     1081 tgatgcgttc ggcttgggat aactatcaat tattcatgcc agaaaaattt tatcttttaa
     1141 atgaggaaca gacaaattta tataaagaag tccagacaat tttttccagc tggtccagcc
     1201 agttacctta tgatctacgg ttgaatccta attgcatgcg ggcttttgtg atcgaattga
     1261 gcggaattct ccgtctgaca aaagaacatc tgactatcta tatcgtcaca aattcggatg
     1321 ttcattatct gatttatcgg gaagccttgg aagctgtaac tactttcgat tttcaagttg
     1381 caccaacaat ttattcaagt atatctgata taaagaagta tgcgcagcag tcctcaaacc
     1441 gggtattatg tgaacgtaca ttatatacac cagatgcggt ccaatatgaa aatattattc
     1501 ctatttcgat caatacgatc gatcgagcta tcatttcagc tgtgcaaaac aaataagggg
     1561 ctgtgacaaa aatcatgtca cagcctctta tttgttgaag atgcccattt ctgtaacaat
     1621 ctcttgatac acgtcaggct cacaacctca cttattttgg acatctacgg gtccgatctc
     1681 tttatttaaa aaataagaaa tgatggttcg gataactacc aacgccgcta gttggaaaat
     1741 atcctgcaag gtaggtttga taatcgattc gatgatccca gctgaaacaa gaatttccaa
     1801 acttagaagt atatagcctc ccaacatctt tttaatagca ttgttctcgc tgactcggtc
     1861 gctttgcact tttgtatgga caaataaata tttcaaaaaa tccttgattg atagacaaac
     1921 accccaaacc aataaacaga tagcaaagat atcaagtccc agtacaagtt tttcgaaata
     1981 tggaagaaga aactgcatca actctgtcgt catcctacct ctcctcttct ttgtttttct
     2041 ttgtcttcta tttcctgatt caagaaataa gaaatgactg ttcgaatgat aataataagt
     2101 gcaagcttca aaatatctgt ccaagtcggc ttgataactg actcgatgat atcagcaacg
     2161 actagtactt ccaagcttaa caaaatataa cttgctagca gttttcttgt atctgcatta
     2221 cgagtattac gttctgcata gtttttcttg acatgagacc aatcaaaaag tttgaataga
     2281 acaagaacga tcccgacaat taagataccg atagaacaaa tatttaaaat agcaatgatc
     2341 cattctagaa attcaattaa atttgagcga aacatatacg ctacctactt tcttttaatt
     2401 tgttcctatt cttttattgt aaaagaaagt taccttgttc gtctatttaa aagttgttct
     2461 aatgataacg gttcgcttaa atttttcttt ttcactctcc catttttctc cctccatcca
     2521 taaatcgtgg aagtatccaa aacaacgtag caagttaaaa taaggcttag tccgtcatca
     2581 gcttttcaaa gaagcactgt ctcggtgctt tttttgtacc caaatttcta aaaatagaac
     2641 catacacctc ctcaaatctt ttcttcttac ctaccctatt tttatttaca ggaaaatcag
     2701 acacttcttc ttttttgtca aaatttcctt tttcttctcg tcctatcatt tggcattcta
     2761 ataaaaacga actatccttg gatatagaaa gagaaaacaa ggaggcgttt ttcatgagta
     2821 aaaaattaat caaagtcggc atcggtttag gtttgcttgc ccttggcgct gcatatctag
     2881 gaaaaaagac aggactattc gaagatgaca gtcatttata tgatgaattc gaatcaatct
     2941 agcatcctat ttagttgaat cactacggac atacttggaa gacattggag gaatcacaat
     3001 gagaaaaagt aaagcaataa tgattggtgc cggtattgcc aatatggcag cggctgttta
     3061 tttgattcaa gaaggcaaat ggaaagggga tcagatcacc ttttattcat tggatgatca
     3121 cggttcaaat gatggtgcac cgaccgacac tgtagtggat gaatattgga ataaaaacca
     3181 tccattagaa aaccaaaaag gctatattgc tcgaggcggt cgcatgctta attaccgtac
     3241 ctatgtagat ttgatggatc tgttaagtcg aataccttct gttactgagc cagggatgac
     3301 tgctgaagaa gatacgagag attttgatgc aaaacaccga acctttgaca aagcacgttt
     3361 gatggaaggt ggcaaaggaa tcattgatgg cggaaaactg ggattcaata ataaagaccg
     3421 tattcttctg actaaactga ttgctatgcc tgattcagaa gaagaaaaac tagacaatat
     3481 cactatcgaa gaatatttca aagatgatcc acactttttt gaaacaaact tctggtttat
     3541 gtgggaaaca acatttgctt tcagaacacg tagttctgct caagaattgc gtcgctatat
     3601 gcatcaaatg atttatgaat tcactcaaat cgaacatcta gtgggcgtca atcgaacacg
     3661 ttacaatcaa tacgaaagta ttatgcttcc tttgatcgag tacttgaaag aacaaggatg
     3721 caaaatcatt ttgaaccgcc gtgtcactga ctgggaattc aaagaaacac cgatgcaaga
     3781 tgaaatcact gtgactggtt tgaaaattac caatacactg actcaagaag aagaatacgt
     3841 tgtggtcgat gaagatacag cagttatttt cactaatggg tccattacag attcagctac
     3901 tttaggagat tacgaaacac cagctgtaga aaatatggat tatggtgcag cttctagtct
     3961 ctggaaaaaa gccagtgaac gtttctacaa cttgggaaat cctgacaaat tctttgctga
     4021 tcgagatgca agcgaatggg tcagctttac cctgacgacg aaaaaccatt tgttgttgaa
     4081 tgaaatcacc cgtatcacga ctcaagaacc aggaaatgcc ttgaattcat ttatttcaac
     4141 cacaccgatc acgccgttag gccaaaaaga tgtcaatatg tctatcgtcg tgcatcatca
     4201 accgcatttt acgtctcaga aaccaaatga aacagttatt tggggttatt tcctttatcc
     4261 aagacgacgt ggcgaatttg tggataaaca gtatattaaa atgaatggaa aagaaatgct
     4321 agaagaactg attggacaac tttcaaaagt agatcctgga ccagtgaata ttcgtgaaaa
     4381 agaaacagaa attttcgaca gcgtaataaa taatatccct gtctatatgc cttatgcttc
     4441 cgcgttgttc aataatcgtg ccaaaagcga tcgaccaaaa gtcattccta aacattcgac
     4501 caatctggcc tttacaggag aatttgtcga gcagccttat cagatgatct ttactgaaca
     4561 aagtgctgta cgttctggag aaatcgctgc tttccatttt gcgggtattc ctatgtcccg
     4621 tcttgtcaaa acaccgcgtt acgacaaaga tattcctact ctagctcgtg ccgcaaaaaa
     4681 aatgtttgaa taatcaagag gttgtgaaaa agctgccctg catcaagtaa taagaacaac
     4741 caggctaaaa cagcttctta tgtttttgat ttggttggac ttattaccgc agatgcaagc
     4801 ttttgaacac cgtttaccaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac
     4861 acccaagcta aaactgcttc ttatgttttt gacttggttg gacttattac cgcagatgca
     4921 agcttttgaa caccatttac caagaggtta tgaaaaagct gccctgcatc aagtaataag
     4981 agcagccaag ctaaaacagc ttcttatgtt tttgacttgg ttagacttat taccgcagat
     5041 gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa ggcgtttagt ttcgagtatg
     5101 aagaaaaatt ttgtgaaaat ggttcctcac gtttttgcat aaatttgact taataccgag
     5161 aaactgatcc cttctgtcat aacctctttt tatttatgtc tgtttctttt tgaaaacaac
     5221 tattttttca acgtttactc tcttgccaat ccaacatcat ctaacggata caactatttc
     5281 aatgattacc attacactaa aaaatcggaa acacacctct ccgaacacaa atacataaga
     5341 ctatagaatg tttcatttaa gaaaaaaagc gtttcattat caaaaaggta agatctcctt
     5401 taaaatgaaa ttatctgtag tactaataat gcagacaatt ttatgaatct gaaaggagca
     5461 tctccaatga aagcagtcac ttggcaagga aaacagaaaa tggaaatcag aaacgtagat
     5521 gatccaaaaa tattagaacc aacagatgca atcatccgta tcacagcaac agccatttgc
     5581 ggctccgatc tgcatcttta ccatcatggt gaagcagttt tggaaaaagg gtatgttgta
     5641 ggacacgaac caatgggaat cgttgaagaa gtgggatctc aagttcaaac attgaaaaaa
     5701 gggcaacgtg tggttatccc ttttaatatt agttgtggtc attgtcattt ctgcctgaat
     5761 cacatggaaa gccaatgtga tgaatcaaat ccagatcagt tatatggcgg gcttttcgga
     5821 tttggcaagt tgaacggtaa tcattggggt ggtcaagcgg aatatctaag agttccttat
     5881 gcagactcta cttcatttgt tgtaccagat aatgatcttc ctgatgaaaa agttttattt
     5941 ctatcagata ttttgcccac agcttattgg agcgttgaaa acgcaggagt aaaaaaaggc
     6001 gatacagttg tgattctagg ttctggtcca gtcggtctat tcgctcaaaa attcgctgtt
     6061 atggctggag cagagcgcgt aatcgcagta gatccagtac agcaccgtct agataaagcg
     6121 gctaattaca atggggttga aacttatttc ttagaagaca cggcacaagc tggaacagaa
     6181 ttgtatgaac tggctaaagg tggcgcagat gtcattatcg attgtgtagg aatggacgga
     6241 ttagagcctg tcaaagaaaa agcgaaaaat ttagtaagct tacaaagcgg cacaatctct
     6301 ccattacaga tggcatcgca agctgtcaaa aaatttggaa cagtccaagt aactggtgtt
     6361 tatatgactc cagcttcttc ttacccgctg caagaatttt ttatgcggga tatcgctgtc
     6421 aagcatggac aggcaccaat tatccatcta atgccaaaaa tttacgcaat gattgcagaa
     6481 ggactattcg atcctacaca aatcattact cattcgctgc cactatcaga agcagcacaa
     6541 gcatatgaga tctttgacaa aaaagaagat aaaaatataa aagttgtttt gaaaccttga
     6601 attcagtaaa aaatacaggt gattttcttt atgcccaaaa tcaaacgaaa tacccatgag
     6661 atcgaaatag gaaaagaaga agatatcgaa atacgaggac gtcgcttccg cttctatttc
     6721 caaaatcgtt acacattgat ctcactggct gtcgatttat taaccgggat tttctatatt
     6781 tttggaagcc ttgcttcctt aactaaaatc cccgatcgat atggcaccta tttttatttg
     6841 gctggtgcta ttttcttgac gattcgtcct attctcagaa tcgtaaggaa catttttatt
     6901 tacgacgaaa aaaaagcaat gaagagaaca agcaaaaaaa aagaggcatg agaatttttc
     6961 aaatctcatg cctctttttt caccctatcg tatcactttg atacagacga gcggtcgtcc
     7021 cactttcttc cttgaccatt gtcaaaaatg tccactcgta tcgttcataa aacgtggtat
     7081 gatctgtaat taagtaaagt ttttcgtacc caagttgatg catttctttt ctgatttccg
     7141 ctaatatccg cttggctatc ccctgcttac gcttttccgg ttcaacaaac agggcacaaa
     7201 gatttggtgt cagatcaggg cggttatgga agtcgttttc aatgacgccc gctcctccga
     7261 cgatggtttc tttttcatct aagaccacat accattgagg aatactttcc ttattagcga
     7321 ttccagtacg aatactttcc tcatacacaa cagcaggaac tccccatttt gatgaaaacc
     7381 aattcgcgct ttgttttact aattctgggt gttctcttaa tttgatccag ttatatgtaa
     7441 ccaatgttta aacctccgac taaaataaaa cgtttacaaa aattatacaa gtctttatga
     7501 aaattagcaa tgacaacaat agtgatgtaa aaaaaaaacg gaaaacaatc acttgttctc
     7561 cgttttttga ttaatcaagc atctaaagct tcttttcttc tttcttggat cgtagctacc
     7621 ttgacgaacg gtagatagat caaaatagaa agaaccaaat tgacagcggc tagtacacca
     7681 cctgcaatac tttttgttgc caatacaccg ccaataattg gtggagttac ccatggaggc
     7741 ataacggtag ctgctggcac taaacctgta gaagtcgcaa agtaagcaac actaactaag
     7801 accattggtg tcaaaataaa tggaatgaac atgatcgggt tcaacacgat aggtaaccca
     7861 aacatcgttg gttcattaat attgaagaca cctggcgcaa tcgacaagtt tgttacaacc
     7921 atatatggtt tgtttttacg tcctactaaa taaatagcta acagtaaacc taaagttgca
     7981 cctgttcctc ctaagttgac aaagctgtca aagaaaggtt tgttcacgat aaacggtgcg
     8041 ctatgcccag ctgcgattgc attaacgttt gcttcaatgg ctggcgcatt gattgtttgc
     8101 atcaaaggat cgaccatatt cgctccgtgt aaaccaaaga accataagaa tggtgtgata
     8161 aatgcaagca gtaaggcaga tggataagag tttgcaagtc ccataaatgg ttcttggacc
     8221 actgtataga aagaacctac gatatcatca acaccgaatg cagcaaaaat tgcagcaact
     8281 aaagcaaacg cagagatagt gatcattgct ggtagtaatg aagcaaatgc ttttgcaact
     8341 gccggtggca caccatcagg cattttgatg atcaattttt catttccgct taaacgtaca
     8401 aagatttctg ttgaaataat cccaacaata atagcaataa acaaaccgtt tgagctcata
     8461 cctaatgctc cgcctaaagc gaagaaagag cctaaagaaa tcacacctgc ggaaacaccg
     8521 tcttttcggt agccgttagc taaatgataa gcaagcaaga aagcaatcaa gacagaaaga
     8581 atggcaaatg taccgttcca agcagcagcc ccgaatcctt tccatgattc accaccaaaa
     8641 attgagttca tcaattcttg gaacccggga atcggtaagt tattgatcat tactgctaat
     8701 gctcctaaga tcattaacgg catactcacc ataaatgagt cccgaatcgc aactaaatgc
     8761 cgttgtgcac caatttttga tgctacaggt ataaaatgtt tctccataaa tgcaacaaat
     8821 ttatccataa atcttccctc ttttctttac tgtagattac gcgttcaatc gacgatacag
     8881 atcaatgatc tcttttgcta aatctgtaaa agctatagat gtcatcagat gatcttgact
     8941 gtggaccgtc aaaagcgtga cttcaacgtg atttccttgt gcttcttgtg tcagcatctc
     9001 tgtttgagag tgatgtgctt ggactaaaga ttcctctgca tctttgattt tttcatctgc
     9061 taaagtgaaa tcaccttttt tcgcagcagc aatcgcttcc attgcatcgc tttttgcatt
     9121 tccgccaaac atgatcagac ccatgatggc ttctagattt ttttgatctt ccatttattg
     9181 tatcctccga taatttttat tatttgttta ttagtttttc tgcttggtcg agtacttttt
     9241 caccattcat catgccatag tcagccatat tgattacatc taaaggaatg cctttaggtg
     9301 ctagcttttc agcaaattga ttttgcataa agcgtacttg cggtcctaaa agtaaaacgt
     9361 ctacattttt agattctagt tgattatccg catctgaagc agatacagca aagatgtctg
     9421 cttccattcc ttttgcttct gctgcctttt gcatttttgt gactaggaga cttgtactca
     9481 ttccagctga acatacaagc atgattgttt tcttttccat tttaaacacg ttcctttcta
     9541 tacttacact tgtatataag caagtagcgt gccaacttta aaagcgcttt aaaaagttga
     9601 tatcatcaca tttaaggtct ataccaagtt gcatacacac taaaaaagaa agcgcacact
     9661 aaacacacaa attaattttt attacctttt ttcttttaac gtgcacaaaa aaagttgtga
     9721 cacaaaactt gtcacaactt ttttcaaata aacggtattc aaaagcttgc atctgcggta
     9781 ataagtccaa ccaagttaaa aacataagat gttgttttta ctttgctatt cttattactt
     9841 gatgcaggac agctttttca caacctctca ataaacggtg ttcaatcagc tgaccctggg
     9901 tattagctca aaacgctgga ttcacaacct cttttatttc aaataaccag taaatacaat
     9961 gaacatctac aaaaaacttc tatttattta tagatttcgt caggcaaatc caattctctc
    10021 ttatcaatca aagtctgata ccacgaagca gattttttca atgttctgct tcgttcatcg
    10081 ttcaattcga ttcgaactaa tccatatcga ttcttaaatg cattcattgg tgacacacag
    10141 tcagtgaatg cccataacat atagccttcg caatttgcgc cttcttctgt tgctttgatc
    10201 aagtaatgca aatgctgtcc gataaattcg atacggtaat catcttgcac gattcctttc
    10261 tcatccatat actgttcttc atattctctt cccataccgt tttctgttat cagccaaggg
    10321 atattatgat aattttcttt tagatacatg gctatatcat acatgatttt cggatagatt
    10381 tcccatcccc tggaattatt cattttcttc ccaggtaaat caaagacttc ataatatcgt
    10441 tcaggatgga aaggtgtctc ttcattccat tgataacgag gggcgcgaac gcgtttagga
    10501 taatattgat tgattccaag gaaatctact gtatgatgct gaatcgtttc taaatcctct
    10561 gttgcaggat cgaaataaat cgattgtttt tgacacaact ccaggaactc ttctgtgtag
    10621 tatcctttta ccatcggatc aaaaaatact cgattataaa acaaatcata catataagca
    10681 gctttttggt cttctggaga attggatcga gcatagacca tttcaggatt taaaatacag
    10741 ccaatccttc caccataatt tccgctgcgg taaatctcta ctgctttagc cgttgctaaa
    10801 actttatgat aattccacat catccacttt tttgtgtttt gttcatgcgg ccagcgaaca
    10861 gcatccaaat agcaacgcgt ttggggaacg attggttcgt tgaatgtaaa ccagtttttt
    10921 accctatcac cgtaacgatc aaaggcaatt tgagcatact tagcataaaa atcaactact
    10981 tttcttgaag accagccatc atatttttcc aaaagaaaag ctgggacttc ataatgttcc
    11041 aaacaaatca tcggttctac gtctgcttcg atcaaagagt cgatcatttc acttatatgc
    11101 tgagcatatt cttcatctac tatacagttc tcataatcag taaaaaaacg agcccaattg
    11161 atagaagtcc gataatgtgt cagatggatg gatttcatca gatccacatc ctgctggtat
    11221 tgttccataa aattagttgc tacggctggg ccatacccat tatgccaaac aaaatgttca
    11281 tttttatacc agctatctat aaacgaatcc tgtccttctt tttttccttt ccaaccttcc
    11341 gtttgccaag cagaagatgc agcaccaaga ataaaatttt ttggaatagt aattttcatg
    11401 atttcctcct gttttttaga tcccatcatt ttccacacga tttgctactt ttacaaatgg
    11461 cagatagatc aaaatagaaa caataataca aacaagttgc gtaataactg caccgatatt
    11521 tcctgctgtt gccagataag cattgacgat cggcggcatt ggccaaggga ccattacaac
    11581 tgctttcgcc gcaaatccta aagcggttgc aaaataacca atcgttccag tgataagagg
    11641 cgttaagata aacggaatag ctaaaattgg attcagcatg atcggcatac caaaaataac
    11701 tggttcattg atgttgaaga tcccaggagc aagggataac ttggtgattt gtttcatatc
    11761 ttcccgtttt ccaactaaca atactgcaat cagtaaaccg atcgtgactc cagacccgcc
    11821 catactcata tacatatccc aaaatggcat agtaataatg ttcggtactt cttttccagc
    11881 ttcaaaagct tccgtattta cagcaattgc cgctaaaagc aacggttccc gtatcggctt
    11941 caccatttga tttccatgga taccaatcac ccaaaagact tgcgcaataa acatcaatag
    12001 caaaataccc ggtaaccctt gtacgacacc ttctaatggg cgttgtacga cattgtaaat
    12061 gatatcataa aggtacattc ctgtaagttc tttgatgata aatcccgcag ccgcgatacc
    12121 cgatacagtc agaatcgtcg ggaataaagc agaaaaacta gcagaaacat tcgttggcac
    12181 ggtatcaggc attctcagct gtagtttctt ctgtttcctc aaccagcaat aaagttcaat
    12241 agaaaggatg gagaccaaca ttcccaaaaa taaccctttc gtatctgtat attgtttagc
    12301 caatacgttt tctactataa cagtctgatt ttcattcact aattcataaa cagttggatt
    12361 aacagataaa taagacatca ctgcgatgat accagggaat acaccatcca ctttattcag
    12421 cttggctaat tcgatcccta tcagaaaaac agcatagatg gtcaagaaac ttagtgtgac
    12481 atagctgatg gcagatgcga taggctttaa ttcttctaaa aaacgcaaga aacttatctg
    12541 tgctaagcca ttttccgagt caaagaccat tgctgaaaac aatgtgccaa aagcacccgt
    12601 aataattacc ggaatcaatg cagcaaatgc gttttttatc accatgatat accttaaatt
    12661 attgattctc gttgccacgt tccctaaaac attggctaat ttatccataa aactcattcc
    12721 agatcccctc attcctgctt cttctccttg acgtcatctc tttttgtcta ttagtcagcg
    12781 cttacatttc gatgaacaga aactatttac tcctcttttt taccaaatat aaaacaaaaa
    12841 ttcatctgat gtatttatca taaaatacat cagatgaact gtcaattagt ttctttttct
    12901 cctattattt tctgtaattc tctccgattt attgccatat ggacagtcat tgcatcaaac
    12961 gcagcaagtg cattccgcct ttcgatggcc tgaacgatct ctgtatgaat atcgatcgtc
    13021 tcgattttca gatgctggat atcataattt ttattaaaca aagcaattga ttcattaatg
    13081 attgggataa tatgcagcaa ggctacgttt ccgctagctt ctgcaatcat cgtatgaaaa
    13141 tcgatgtcta attgaacatg ttcttgtgtg ccttctcgaa aagaagtttc aatcttttgc
    13201 gttaattttt tcatttggac gatttgattc ttcgttgcat attccgctgc caaacctgcc
    13261 atcttaggct ccaataaata tctcagttca aataagtctc gtatcatccg atcctgatct
    13321 tcaatcaaag aaaaaccgag tgggtcattg gcaatccctc gcttatcact gacaaatgtc
    13381 cccgctcctt gccgaacttc aaggatattc ctggaagcta gagcccgcac agcttctcgc
    13441 accgtgcttc gccccacctc taaataatcg gacaattcat attcattcgg cagtttatct
    13501 cctacttgca actgcatttc ctttaaatat ctaaggatct tgtcagtagt tacttctact
    13561 agggttttat tttcttccaa accacatacc tccttttcat ctgatgtatt ttttcgtaat
    13621 tattataaca cgtttgaatc atctgatgat tttttgtata caaaattgat taatccgttc
    13681 atagacatac aaacgaagaa gatatttacc ataaaaaaga ctagga
//
LOCUS       DANKHL010000067        13463 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000067 DANKHL010000000
VERSION     DANKHL010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.070"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            67..2265
                     /locus_tag="PW101_001388"
     CDS             67..2265
                     /locus_tag="PW101_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2513792.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            complement(2847..3719)
                     /locus_tag="PW101_001389"
     CDS             complement(2847..3719)
                     /locus_tag="PW101_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2513793.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            4019..4993
                     /locus_tag="PW101_001390"
     CDS             4019..4993
                     /locus_tag="PW101_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2513794.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            5015..5707
                     /locus_tag="PW101_001391"
     CDS             5015..5707
                     /locus_tag="PW101_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2513795.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            5673..7181
                     /locus_tag="PW101_001392"
     CDS             5673..7181
                     /locus_tag="PW101_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2513796.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            7255..8358
                     /locus_tag="PW101_001393"
     CDS             7255..8358
                     /locus_tag="PW101_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2513797.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            8361..9698
                     /locus_tag="PW101_001394"
     CDS             8361..9698
                     /locus_tag="PW101_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL2513798.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            9685..10563
                     /locus_tag="PW101_001395"
     CDS             9685..10563
                     /locus_tag="PW101_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HDL2513799.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            10560..11483
                     /locus_tag="PW101_001396"
     CDS             10560..11483
                     /locus_tag="PW101_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HDL2513800.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            11480..11890
                     /locus_tag="PW101_001397"
     CDS             11480..11890
                     /locus_tag="PW101_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDL2513801.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            11894..13462
                     /locus_tag="PW101_001398"
     CDS             11894..13462
                     /locus_tag="PW101_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL2513802.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
BASE COUNT         4502 a         2218 c         2848 g         3895 t
ORIGIN      
        1 attagatgag tatcaatttt acagaaaaat gaaagccttt tattttaaaa ggaggagagg
       61 aagaaaatga agaaaagtac agtattttat aaaatatttt taccgatgtt tttattagga
      121 gccgtacttg tcataggatt tagtttattc atttaccaga atacctatga atcgatcgaa
      181 tcgagttatt taacagacaa aaagaatttg ctcaggcaag ttaaaactaa cgttgaatgg
      241 aaaattagaa cgattgaata ttcgttttct acgtatggtt cgaccaaaaa tttctcggac
      301 atattcaagc atccgctgac atatacagat tactccactt attctgaaat caggaaagaa
      361 ctaaatttta tagaaacgat tgttatggat gataataatt atgatttagt cagtttggct
      421 ggtaaatggg gagtaatcaa tggatcgttg agtcaactga ccgaagagga agttgaaaag
      481 tatcaacaga agtacataaa taacaaaaat aatttatttt ggcaaaagaa aaacaaagga
      541 atcgaaatgg tgatttcttt gccaatgttt gaaaaagaaa aatatgcatt gggaattgct
      601 acgatagaga aacatacaat agaacaagtt gttggtgaaa gtggagaaga catactttct
      661 attgaaaacg aagttggcaa cctttttact actcagacag taaaaaataa agagcaacca
      721 actgtattaa cggacgtgga tctagattac aatcagctga aagtgataag agaaaaagat
      781 aaaacgtata tagtactgaa atcagattat aataattgga tttacagatt agaagttgac
      841 tctagtgcaa ttggcgcaat gatccgcaat ctgagaatcg ggctagttgc agtgtcttta
      901 acactggtag gactcattgg tcttttttcc tacttatttt cggacagatt tgcccgtccg
      961 atttctcaaa tacaggaacg tctgaatctt aaatcaaaag gattcagtgg gaaagaacta
     1021 gatttggtcg ctcaatcagt cagtcggatc ataggagaaa aagaagcgtt aagtgcacac
     1081 ttagttaccc aaaagccaca gctggaaacg ctatttgtac ttagtttatt tagaaatcga
     1141 gtagaaaggc gagaactaga tcagcggttg cagcaatttg gttatagttc tcaaaatgac
     1201 tgctattata cagctttggt tcaaatcgat ctgttagaag atagtcacgg tggggagcgt
     1261 gatttgctac ttttagcgat caacaacatg atcacggaaa ttgtgccaaa ggaaaatcga
     1321 atgctgccga tcgttttgaa tgaagaaatg caagccacta tttatcgaat ggataaagat
     1381 gatgcagaag caagcaaaaa agtcatggag tattgccgac tcattcaaaa acgtgtaaaa
     1441 gaatacttga atttaactat cagtataggc atcagtaacc ggttcgaaac gctgaatgaa
     1501 agcaagcaat cggttgatcg ggcaaaagaa gccctgtatt atcgtgtaaa tactggtcca
     1561 agttcaatta ttttttatca ggaaatcgtt cctgctgctc atgaaaaaac acttattaga
     1621 tatccgatag aagaacaaaa tcgattgttt gaagccattc gttctggaaa tcaggaagtg
     1681 aagaatttag tacatgaatt gattgatgca ttgtttgcac aaaataaaaa tcctttgagt
     1741 cgggaagtag tttcggtccg cttgatcaat gaactcgttc aattaggaca gttattggga
     1801 gtagattcaa aaaactttga taacatgaaa caaatttatg taaaagcact gaacaattat
     1861 catcccaaag aactagaaaa acttgtttta gaacaattag ttctgccaat cacagaatgc
     1921 tctcaagcaa tcactgatca tgaatttaaa aatttgtcgg agaatattat gcatatcgtc
     1981 tacacagaat atgatcgtga tctgtcttta gatgtgatcg ctgataggtt gcattataac
     2041 cctaactatt taagtaatgt atttaaaaag gaaacaggag aaaaatttgg tgattttgtt
     2101 caaaatcatc gtttagaggt agcaaaaaaa tggttgaaag agactaacct ctccgtaaaa
     2161 gagattgcag aaagattgca atatcgaaat ccgcaaaatt ttattcgttt ctttaaaaag
     2221 aaggaatcga ttacgccggg agaatatcga aaaaaatatt tgtgaatcaa gaggctgttt
     2281 ttcttgtgat ctgattcaag caataaaaga ggacagcaaa aaacagcact tcatgttttg
     2341 ctgctggctg attttattgc cgcagaatca acaagataaa cgccgtttaa tcaagaggct
     2401 gtttttcttg tggactgatt caagcaataa aagaagttag aataacccta gtatttgtat
     2461 ttatggatag ttaaatttca aggctaagca cgcaaaaaga actgtgacaa aagtcttgtc
     2521 acagttcttt tttacgaata aatggcgttc aagagacagg ctatcgacaa taaggcataa
     2581 ttttgtgaaa tatgggaagc tatttttcaa aatcgcttct tattgctcct gtgggttact
     2641 cgtcgcaatg tttgtgagag tttttatcat cgctcaaggc tgaacgtctc tttcacaacc
     2701 tctgatacac ggtgttccag gagtagattc ttcgaaaaat aagccgaaat ttcacaaaaa
     2761 tttaagaagc aattttcgta aatgccttct tatttcttga agctgaccac ttctgacaca
     2821 actaccattt tgcgttcagt acataatcac cctttgacag aacctacgag tgctcctttg
     2881 acaaaatatt tctgaacaaa aggataaaca atcagcatag gcactgttgc aataacgata
     2941 acagccattt taactgtttg tgctggtgga atcacatcaa cggcactact ttctgcttgc
     3001 atcccacttg atacaatgac gatttggcgt aaaagaatct gtatcggcca tttagatgaa
     3061 tcgttgatat acaaaatagc agtcatataa gtgttccagt aagtaacagc ataaaaaagc
     3121 gagatagtag caatagacgg taaagctaat ggcagcataa ttttgaaaaa tacaccaaaa
     3181 tctgtgcaac catccatttt tgctgattct tccaaactat ccggcaaggc ttggaaaaag
     3241 tttctcataa tgatcatatt cattgcatta acagccacag gcaggatcat ggcccagtaa
     3301 gaattgacca gccctagaga acgaacaact aagaacgttg gaatcatccc cccgctgaac
     3361 aacatagaaa atacaacaag aaagttgacg aaacctcgac caaacaaata ctttctggaa
     3421 agaccgtagg ccatcagtga agttgcacac atactgacga tcgttccgac acctgtaaca
     3481 ccaatcgaaa cagcaagtgc tctaaaaatg gtcggcgtag aaagaatgta gcgatacgcg
     3541 tctaaagaaa aggtagtagg aaaaaggata aactttttcg cgacgacttc ttgtgtagaa
     3601 gcaaaagatg tagcgatgat atttaaaaaa ggaacgatac agatcaaagc tagcaaaatt
     3661 agaaacgtat tgttgaaaag tgtaaaactt ctttctccta tagagacttt ctttttcata
     3721 tagataggct cctcctcaaa ataaattaaa tgtttctgtt tctatatgtt caatttatca
     3781 tcggtgaaag attacgtata taatcatttc ctatgctaag tgtcagatag aaaaaaatag
     3841 aaggtttgct atgcgtagtt tatttgtgaa aaaatacact ttatcgtttt gtatagcaca
     3901 aaaatcattg ctatgtaagc gtttttactt acggtaaaaa tgattatata cgtaattcag
     3961 atagttatct atcatgaagt cataagcagc aaacattcac aaaactaagg aggggcttat
     4021 ggaaagtgta aagaaacaag aaccatttca actgacgcta tcgaaaaaag aagaaaagct
     4081 cagaagacga aaaaaaataa ttgcaggaat caaaacgaat aaatttttat atgtgatgat
     4141 tctgccggga gtcctttatt tcttgatttt taggtatctt cctatggggg gactcgttat
     4201 tgcttttcaa gattatcaac catttttagg aataatggga agtccttggg tcggtttcaa
     4261 acatttcatt cgtttgttta cagaaccgac attctttaca ttattaagaa atacgttgat
     4321 cttgttcgga ttaaatatct tcgtgttttt tccgatgcca atcattttgt ctttactatt
     4381 aaatgaagta acgaacaaac gattgaaaaa cgggattcaa acgatcattt acatccctca
     4441 cttcatgtct tgggtaatca ttgtttcgat tacttatgta tttttgaatg ttgacggagg
     4501 ggtattgaat gaattccttg caacaatggg atttgaaaaa atcagttttc taacatcccc
     4561 tgaatggaca cgcacagtat atatcggaca agtgatctgg aaagagttgg gctggtctac
     4621 gatcatctac ctttcagcga taactgcagt cgatacacag ttgtatgaag ctgcagaaat
     4681 ggatggagca ggacgcttac gcaaaacatg gcatgttaca ttgccggcaa ttcgtccagt
     4741 aatcattacc ttgctgattc taaaaatcgg tcagacatta gacttagggt tcgagcatat
     4801 gtacttgtta ttgaattcat tgaatcgaag cgttgcagaa attttcgaca cctatattta
     4861 tacagctgga ttgaagaatg gacagttaag tttcagtaca gcaatgggat tgtttaaagg
     4921 ggtcgtagga ttgtttatgg taattttagc taataagatt gccaaaaagc taggagaaga
     4981 cggcgtttac taagatagaa agagagggaa agatatgctg cagtcattag ataaaataaa
     5041 cggaaggcta aatcgtattt tacagttggt ttatttgaat tttctgtggg gcggtttagt
     5101 catcgcaggt ttgggagttt ttactttcgg tcctgcatcg tatgcgatgg ttgcagtgat
     5161 tagaaaatgg ctgcgctcaa aagaaacgtt tcctttgacg aaaacgtact tcacatattt
     5221 taaagaaaac tatcgtgaaa ccttgctgat gagctggata tatggcgcca tcgggattgt
     5281 tttacttgtg gatctttctt tcatacacaa ttggtatctt aggatcgctt tgtatattgc
     5341 tgcgttcata tatgtgctct cagcactcta tattttccca atcatggctc attatgattg
     5401 gaaaggatat tttttcaaaa tgaaaatggc atttttattc ggattctcac atcttcatta
     5461 ctcattagtg ttgatattaa gtatggtagg gatttatctg atgatcatcc aaacaatgcc
     5521 aggtgtttta acctttatcg gtggcagtct cttcttttcc gcagtcacgt ggacagcaaa
     5581 ttctatattt tgccatttgg aatggaagaa ctcatataca gaaaaaacag aagaaaacaa
     5641 aaatattatt aaaacagtga aggagaaaat ttatgaaaaa agcaacgctc gtaaaattag
     5701 gtcttagttt tgcaggaatt ctagcacttg ccgcatgcgg cagcagttca agtaacagtt
     5761 cttcagagac agaagatgga aaagctaaaa tcacttggtt aaatattttg catcatgctt
     5821 caccgcctac agacacgatt gtgaacttga tcgaagaaaa aaccgatacg gaactgacat
     5881 tcaactggat tcctgatgct tcaaaagaag aaagattaac aactgcattg gcttctgatg
     5941 aactaggaga tatcgttaca cttcaaattg cgatgttgaa aaactcttct gtccgcaatg
     6001 cgttaaaatc aggcgtattc tgggatgtat cagaatattt aaaagactat aaaaatttaa
     6061 gtcaaatttc tgaagataga atcgaagctg cgagtatcga cggtgccttg tacggcgtac
     6121 cgatacaaaa agactacgct cgtgctggct tagtcattcg aaaagactgg ttggataatc
     6181 taggactaga tgttccaact accatggatg agctttatga agtagctaaa caatttacag
     6241 aaaacgatcc tgatggaaat ggtgtggacg acacagttgg ttttggcgat cgtgcgccta
     6301 gtgatattcg ttataccagc tttaaacttt tcacttctta ttttggagca ccaaatggct
     6361 gggctgtaga cgataatggc aaattcactc cagaatttga tacagaagag tatatgaaag
     6421 ctttgaattt ttcaagagat ctatttgaga atggctattt ggcacaagac tttgcagtta
     6481 cccaaaagac tgatcagcaa gaacaatttg cacaaggaag aactggaatc tacactggaa
     6541 tggtggatat caagaatctg aagacgatgg gtcaaggtat tcaagatgat atggaattag
     6601 tgcctgtaaa caagatctca gatggtgaag gcggcggtta tcatgtttgg tcagaaggaa
     6661 atggtattgg cggcttgcta gcatttccca aatctcaagt aaaagacgaa gcacaattga
     6721 agaaattact tcaatttgtt gatgatcttt tagaagaaga tgtgtataaa ttgatgactg
     6781 gcggtatcga aggaacgcat tatgaattga atgatgatgg ttctatcgaa tttatcgata
     6841 tggatctttg gcaacaagag gtgcaaccgt tatcaagctc tcgtcctagt gaagtgacgt
     6901 tctctttcaa agatacagac cctgaaaaag aattctctaa tcaattgatc aaagaaaatg
     6961 aagaatttgc tgtaatggat ccttctgtgc cattggattc accgacgaac aatgaagtag
     7021 gatcagaaat tgaaaaaatc gtcattgatg ctacggtgca atacatcatg ggacagcttg
     7081 acgaagatgg attcaaaaaa gctgtagaag actggaaagc acaaggaggc gatcgaatca
     7141 cacaagagta cgaggaagca tacaaagcag cggcacagta atatagacga ttgggacaaa
     7201 tacttcttgg tatttgtccc aaatcctatt ttaattttta aaggagagca aataatggaa
     7261 aactggtcaa agaaattagc aaaaagtatc atggaaagaa cacctcgctt atatgaagaa
     7321 aagtggtata aaggaaaatg gtcttatgac tatggcgtcg tacttaaagg gttccaatta
     7381 ctttgggaac gaacccaaga aaaaatttat tttgatttca taaaagacaa tatcgactat
     7441 ttcgtgcaag aagatggaac gatccgtggc tacagcgttg aagaatacaa tatcgaccat
     7501 gtaaatacag gaaagctttt ctttttattg tacaaagaaa caggcgaaga aaaatataaa
     7561 aaagcagcag aactcctttc ccagcagttg gcaaaccatc cacgcacatc cgaaggagct
     7621 ttttggcata aagaaattta tccttatcaa atttggttgg atggcttata tatgggttct
     7681 cctttttatg ctgaatttat ccagaatttt agtgaagaaa aagattacca agatgttttg
     7741 agacagtttg aaatcagtta taaacatcta ttcgatccaa aaacaaattt gctgattcat
     7801 gcatgggatg aaaagaaggt ccaaccatgg gctgatccaa aaacaggtct atcccatcat
     7861 ttttggagcc gatcgattgg ctggtatttg atggctgcag cagataccta tgaattactt
     7921 catgaagaaa cagattgttc tttgctgaaa gaaattctag aaaagacact ggctgcatta
     7981 cttccatatc aagaagaaaa cagcgggaca tggtatcaag taacaacaga aacaaaacgg
     8041 aaaggaaatt atctggaagc ttccggcagt agtatgtttg tttacgcaat ggctaaagga
     8101 atacgtttgg gactgctgaa caaagaagaa tggcttccac aagtgaagaa atcacatcaa
     8161 ggattacttg atgaatttgt tctggaaaca aaagaaggct ggctgaactt gaataaaaac
     8221 tgtcaagtag cagggttagg cggagcagat cagcgagatg gttcatatgc ttactacatc
     8281 agtgaaccaa ttatttgtaa tgatcaaaaa ggcgttggtg cattcttgca agcattgatt
     8341 gaagtggagg cattataaat atgtatgata ttttgacatt tggcgcatca attgatgaat
     8401 tgaatactga agcaatacaa caagccattg atgctgcagc aagtgacgga ggcgggaccg
     8461 tagttgttcc agcaggtgaa tttctaacag gagctttatt tttaaaaagc aatgtcgaac
     8521 ttcatttaag cgcaggagcg gttctgaaat tctctgacga tccaaaagat tatcctgttg
     8581 ttcattcacg ttgggaagga gtccatcgaa aagtttatgc ttcctgtatc tatgctcaaa
     8641 atgtagagaa catctctgtc actggtttcg gtacattaga tggaaatgga aaaaaatggt
     8701 ggcatacttt tagaaatgaa ccagataatc tcgcttatcc tcgtcctaaa ttgatgagtt
     8761 tccataactg tcatcgaatt actgtgaaag atattaaatt gatccagtca ccaagttgga
     8821 cgatcaaccc gattctttgc agtaatgcaa cgtttgataa cttgacgatc ttgaacccag
     8881 cggattcacc aaatacagac ggaattgatc cagaatcatg caaaaatgtg cgtattagta
     8941 attgccatat tgacgtggga gatgactgta ttgcaatcaa ggcagggact gaagatacgt
     9001 acgaaaggat cgcttgcgaa aatatcacga tcacaaattg cacgatggtc catggacatg
     9061 gcggtgtcgt attaggaagt gaaatgagtg gtagtattcg taatattacc atttcaaatt
     9121 gtatcttcca agaaactgat cgaggaatac gattgaaatc tagacgaggt cgaggcggga
     9181 tcgttgaaga tattcgagta tccaatatag tcatggataa tgtgatgtgt ccatttatac
     9241 tgaatcttta ttatttctgt ggtccgcgag ggaaagaacc gtatgtatgg gaaaaaaagg
     9301 cttatccgat agatgaacgg acaccagcat tcagaaggat ccatttttcg aatatcacgg
     9361 caaggaatgt ccatgcttct gcaggtttta tttatggatt agcggaacaa ttcatacaag
     9421 aaatcacatt tgacaatatc gatgtctcga tggcccaaga tgcagttccc ggtgttcctg
     9481 ctatgatgac aggaatagag gaaatgagca gacaaggatt ttatattggc tttgcgggta
     9541 aggtttcctt taaccagatg tcgattgaaa atcacgaggg accagcattt ctattagaaa
     9601 atactaaaga aatcagtatt gagaaattcc aatcaaatca atcagaaaat atggatgaac
     9661 tggttgttcg taaaaacttt gcttatgcag ttgaatgatt ttttaggtgc tccatctagc
     9721 agacataccc ctagtggaaa acgattatac cgagaaaaac aaaacggttt tatgcttgaa
     9781 tactatcaat tgtacttaaa tgaattggaa acagtaccag ccctctatgc ttatccagaa
     9841 aaagaaggac cgtttccgcc ggtcctttac ctgcattctc acggcggaga tttttcagtc
     9901 gggaaaagtg aattgatcca tggtgctcct tacttagctt ctccatcttt tgcagaagta
     9961 ctgacaggaa tgggatatgc tgtttggtcg attgatgctt ggggatttga agaacgcgga
    10021 ggtatttctg aaagcgagtt gttcaaacaa tttttattaa caggaaaaac gttatgggga
    10081 atgcgtattt atgatgtgat cagcttgatc gattatttgg agacaaggga agacacagat
    10141 actcaacggc tcgcaacgat tggtctttct atgggaggct tattaagctg gtgggtgaca
    10201 gcattggatt ctcgtgtcaa agtttgtatt gatctagcgg cacaggtgga catcgaaata
    10261 cttttgctac atcgccgcct agatcatcat gggttttatt actatgtgcc aaatctattg
    10321 acagcttata caacgttagc aatccaagaa aaaatcgctc cgcgccctcg tcttagctta
    10381 gttggaaaaa acgatacgat gtgcttggac gaaggcgtac tgaagctgag aaaagggctg
    10441 gacaaaaagt atcaatcaat gggtagtcca gaaaactttt cttctttttc tttaacaggc
    10501 ggtcatatgg aaacacaaga aatgagaaat atttggaaac aattcataag ggagcattta
    10561 tgaggatgaa agacaaactg ataattggaa agaatgctat agataccccg acggattttg
    10621 aaactatcca agaaggaatc gattatttgg caggcctgcc ggatagccat ccaaaggtct
    10681 tgatagtaaa agaaggaaga tatgatgaat atatagaaag cagattatcc aattttaaac
    10741 ttgtgggagc aggaacagta gttatctccg gtaatcgttt tgcaaaacag gagtatcttg
    10801 acggtagcca gttagggact ttccgaagtg ctactttttt cttagaagga tcgaatgtcg
    10861 tattagaaaa tcttcacatc gaaaatacag caggtccagg agaaaaggta ggacaggctg
    10921 ttgctttatt caattcagga gatcgcgtat ctttaaaaaa ctgtcgacta tctggttatc
    10981 aggatacctt atgcacgggt ccgttgcctg atttgcaaaa agatggtact cctttttgta
    11041 caccggcttc tgccaatcct gagtattgtc gtcagaccta tgaatattgc tggattgaag
    11101 gaacggtgga tttcatattt ggtggggcag atgccgtttt tcatcagtgt catatccata
    11161 gcttagccag taaaaatcct ggatacatca cagcagcatc tacgccgaaa aatcaaaaaa
    11221 cagggtatcg ttttgaaagc tgtaccttga ctgcagaacc tgatactaga aacgtctatt
    11281 taggaagacc gtggcgtccg tatgcaaatg tcttatttga agcgtgtaag ataggtgggc
    11341 atgttcaccc aactggttgg gataattggg atgacaagaa aaatgaacag actgtcaaat
    11401 tctctgaacg taataacaat tatgaaacat cgatcaaaag acctgaatgg atacatttag
    11461 aaaagcaggg ggttcagaaa tgaaaagaaa atgggaacta ttgctaggaa tggtcggtgg
    11521 cagtctatct ttgattttct ttggcgggtt agcagttact ttaagtaata tgtctgcgtc
    11581 agagtttaaa aaaagttatc agtcgcttgc tgtagatcat tcaactttgt ctttagaaaa
    11641 tacatttgga ttattacagg acatgactgg tctgtttgcc gttgtgttgt ttatttcact
    11701 tgctttttta gcagtagcat tgtttttgac tgctaaagga aaatacttaa caactgccac
    11761 tggtttgtat tttataacag gattcatatt gttgattggc actcagttta ttgcgtttcc
    11821 ttttgcattt ttttatttcg cagctggggc attcagttta tatcgagtaa gaatgagaaa
    11881 aggagcctga atcatgcaga ctatcaaagt gaaaaatcct attttaagag gattcaatcc
    11941 agatcctaat atattaagag tgaaagagcg gtattacatt attgtctcct cttttgaatg
    12001 gcttccagga atccgagtat atgagtcgaa aaatttagtc aactggtcac atacaacgga
    12061 tattttgacc actcaagtat ctttgcaagg aaatcctaca aatggaagta tatgggcgcc
    12121 acaaatcagt tatgctgatg gattgtttta tcttgtttac acagatgtca aaagtgcagg
    12181 ccgtccgttt aaagatgtcc acaattattt gatcactgca tctgagatca caggtccttg
    12241 gtccgaacca atttatttaa atagtaacgg ctttgatcct tctttgttcc atgatcttga
    12301 aacgggaaaa aaatggcttg tcaatgaact atgggattat cgtctggaca caccgaataa
    12361 atccagcggt atcgtattac aagaatatga tgcagagaag aagaagcttg atggtcccat
    12421 ctataaaata tttgcaggta cagaattagc gaaaacggag gcaccacatt tgtattttcg
    12481 taacgggtat tattatttga tgacggcaga aggcggtaca ggaagaggac attcagttac
    12541 agtttgccga gcaaagaata ttactggtcc ttacgagctt gatcctggtt ttcctatgct
    12601 gacagctagt gacaaacctg cttctacatt gcaatgtaca ggacatggga gcttagtaca
    12661 aaccccgtca ggaagatggt acatgacata tctttgtacc agaccattaa acggtgcggc
    12721 tattttggga agagaaaccg ctattcaaga agtgtattgg acgtccgacg attggttaag
    12781 acttaaagat agccagacaa ctccgatgga agaaataatg attgagacaa atgaagaggt
    12841 acaacaagaa aaagatcatc agtttatgga tacatttgaa ggagagctaa agaagaactg
    12901 gaatgccaga cgcattttgc caaatgctga ttggtgtgat acaaaatgta gaccaggttt
    12961 tttacgtctg atttccggtg aatctcttca atctaccttc gaacatcatt tgttagctat
    13021 tcggcaaacg gattttactt ttgatgcgga gacagcattt gaatacatgc ctaataattt
    13081 taatcagatg gctggactgg tactgtactt aaatgaatcc aattatattt attgttatgt
    13141 tacttggaat gaggacaagg gaaaatgcct acgtatgata gaatcagaaa acggagtagc
    13201 acaaatagaa gattggacga ttccactcac agaaaagaag acttttttga aagtcactgt
    13261 aaagaaagag acagcacagt ttcatttgaa agacgtatca actgaagaat ggaagaaagt
    13321 aggtccgcaa aaaaacatgt gtatactctc aggagggttt actggaaatt ttatcgggat
    13381 agcagtgcat gatctaaata aaaaaagagg aagttatgct gattttgagt ttttcaagta
    13441 tgatggaaaa gatcatctgt aat
//
LOCUS       DANKHL010000068        13403 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000068 DANKHL010000000
VERSION     DANKHL010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.071"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            333..947
                     /gene="gmk"
                     /locus_tag="PW101_001399"
     CDS             333..947
                     /gene="gmk"
                     /locus_tag="PW101_001399"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL2513803.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            952..1257
                     /locus_tag="PW101_001400"
     CDS             952..1257
                     /locus_tag="PW101_001400"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL2513804.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1510..3918
                     /gene="priA"
                     /locus_tag="PW101_001401"
     CDS             1510..3918
                     /gene="priA"
                     /locus_tag="PW101_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDL2513805.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3933..4424
                     /gene="def"
                     /locus_tag="PW101_001402"
     CDS             3933..4424
                     /gene="def"
                     /locus_tag="PW101_001402"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL2513806.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4417..5355
                     /locus_tag="PW101_001403"
     CDS             4417..5355
                     /locus_tag="PW101_001403"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDL2513807.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5355..6713
                     /gene="rsmB"
                     /locus_tag="PW101_001404"
     CDS             5355..6713
                     /gene="rsmB"
                     /locus_tag="PW101_001404"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDL2513808.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6726..7466
                     /locus_tag="PW101_001405"
     CDS             6726..7466
                     /locus_tag="PW101_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDL2513809.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7463..9532
                     /gene="pknB"
                     /locus_tag="PW101_001406"
     CDS             7463..9532
                     /gene="pknB"
                     /locus_tag="PW101_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDL2513810.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9697..10596
                     /gene="rsgA"
                     /locus_tag="PW101_001407"
     CDS             9697..10596
                     /gene="rsgA"
                     /locus_tag="PW101_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDL2513811.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10609..11259
                     /locus_tag="PW101_001408"
     CDS             10609..11259
                     /locus_tag="PW101_001408"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDL2513812.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11260..11898
                     /locus_tag="PW101_001409"
     CDS             11260..11898
                     /locus_tag="PW101_001409"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDL2513813.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            12053..12907
                     /gene="mreC"
                     /locus_tag="PW101_001410"
     CDS             12053..12907
                     /gene="mreC"
                     /locus_tag="PW101_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDL2513814.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12911..>13403
                     /gene="mreD"
                     /locus_tag="PW101_001411"
     CDS             12911..>13403
                     /gene="mreD"
                     /locus_tag="PW101_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDL2513815.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKK"
BASE COUNT         4429 a         2478 c         2963 g         3533 t
ORIGIN      
        1 gtattaagcc aaatttatgc aaaaatgtga gaaactattt tcacaaaatt tttcttcata
       61 ctcgaaacta atcggcttct tcacgacctc ttatacacgg cgttcaaaag cttgcatctg
      121 cgataataac cccaaccaaa cgaaaaacat gagaagctgt ttatgtttgg ctgttcttat
      181 tacttgatgc aggacagctt ttttacgacc tcttggtaca gggtagcttt tttaccaatt
      241 tctagctata attaaagata accttgaaag ttgcagaaaa aaagtgcaaa ataaacgata
      301 agtacaaaaa gactagttga aaggacgtcg tcatgtcaga gcgtggatta ttaatcgtgt
      361 tgtctggtcc atccggtgta ggaaaaggca cagtacgaaa agcaattttt gagagtgagg
      421 ataatgattt tcagtattcg atctcaatga ctacccgtaa aatgcgtgaa ggggaggtcg
      481 aaggcgttga ttatttcttc cgaagcaaag aagagtttga atcaatgatc gaagctggag
      541 agatgctgga atacgcagaa tatgtcggga attattatgg aactcctttg tcatatatca
      601 acaagacatt ggatgaagga aaagatgttt tcttggagat tgaagtccaa ggagcaaaac
      661 aagtcaaaga aaaagtgccc gatggtgtat tcatcttttt gactccgcca gatttagcgg
      721 aattaaaatc acggatcgta ggccgaggaa cagatgcaga tgaagtaatc gaagaacgaa
      781 tgaaagttgc aaaagaagaa atcgaaatga tggctcttta cgactatgct gttgtaaatg
      841 atgaagtgcc gctagcagtt gaacgaatca aaaacattat tgccagtgaa cattttcgag
      901 tagatcgcgt aatcggtaaa tatattaaaa tgttaaagga gatgtagtct tatgatgtta
      961 aaaccttcaa ttgactcact attagatcgt gtaaattcaa aatattctct agtgatttta
     1021 gcaagtaaac gcgcgcatga attggatgca agtgcacagc ctactctaga ttcttttgat
     1081 tcagtaaaaa gcgtcggtca agctttagaa gaaatcgatg caggaaatgt agtcaatgat
     1141 ccacatcctg aattaaaacg tgaacgtttg aagatggaag aagaggaacg tcaagcacaa
     1201 aaagagcgtg aacaacacga attggaatca cgtatccgtg aagagcaaaa aatgtaataa
     1261 agaaaaatag agcgaacatc taaaaagaaa agcaaaccca gctgtttcct tttcaaacat
     1321 tcgccactta ttgtctggta gaaaaggctg tgtctgaact ttttgacaca gttctttttt
     1381 ttcttatctg ctgttaattt tttgttagga tttggcagaa gataagacaa atccatcgat
     1441 ctctagtaaa ataggcaagg aactttttgt cgcttgataa gaaaagacaa aaaaaggagg
     1501 aaacgaacca tgacattaac agctgaagtc attgtggacg tacctactat gcagaccgat
     1561 cagccattca cttatttggt tccctctgaa atggaaacag ctttgcaagt cgggatgcga
     1621 gtagaagtcc cttttggtaa tggcaatcgt catgtccaag gatttgttat ggctatccaa
     1681 aaatcagaag aatcagctaa tccatctttg aaggcagtta ttcgtttgct agaccttgct
     1741 ccggtggtca atgaagaatt actttcatta gctgattata tgaaaaaaat tacttacgca
     1801 ttcaaaatca cttgtctaca aacgatgctt ccgagtgtga tgaaagcaga atatgataaa
     1861 ttgatctatc ctttggcaga cacaccagaa gttcaggtat tgtttgatca gcgagaagtg
     1921 atttcctgga aggaagcaga agaagaaggc agcctttctc aattgattcg ctggcgccaa
     1981 gagcaattag tagatatcaa atatgaagtc catacacgaa ataaagtaaa aacgattcgt
     2041 ttggtccgtt ctttgttaac tgaaaagcaa atcgaagaag aatgggcaaa attacggcaa
     2101 aatgccaaga aacaaaaaga acttcttttg tgtttatctg aactgagcca agaagaacca
     2161 attgcttatt tcaaaaataa aggaatcagt actgctgtct tgaatcaagg aaaagaaaag
     2221 ggctggctgg agtttataga atcagaacgt tatcgcgacc cttataaaga ccgagtattt
     2281 gaccaaacga cggcattgga actgaatgca gaacaaaaaa atgctgtcga acaaatcatc
     2341 acagcaggtc agcagcaaaa agatcaggtc tttttgctgg agggaatcac tggcagcggg
     2401 aaaacagaag tatatcttca ggcaatcgct gatgttttgt cagaaaataa aacggctatt
     2461 atgttagtgc ctgaaatcgc attgactccg caaatggtcg agcgattcaa aggacgcttc
     2521 ggagaatcag ttgcggtctt acatagcgga ctttcccaag gtgaaaaata tgatgaatgg
     2581 cgcaagattg agcgggaaga agcgcaagta gtagtaggcg cacgatctgc tatttttgcc
     2641 ccgctgaaaa atatcgggct gatcattatc gatgaagaac atgaatcaag ttacaaacaa
     2701 gatgagacac cgcgttatca tgcgagagat ctagcaattt ggcgcagtaa atatcatcat
     2761 tgcccaatag ttctaggaag cgcgacgccc tctttagaat caagagcacg agctcaaaaa
     2821 ggcgtttatc agttattgca gttaaaccac cgagccaaag cagctgcaca gcttccagct
     2881 attgagatcg tagatatgcg tgaagagttt cagaatcatc ggacctctac tttttctgct
     2941 aatttgcagg aaaaaataca aaatcgatta gacaaaaaag aacaaactgt cctactgctc
     3001 aatcgtcgag gatattcgtc ttttgtgatg tgtagggact gtggatttgt tcttccatgt
     3061 cctaattgcg atatctctct tacactgcat atggatacac gttcaatgcg ctgccattat
     3121 tgcggacatg aagaacctat tcccaatcgc tgtcctaatt gtggcggcaa taaaatccgc
     3181 tactatggaa caggtactca aaaagtagaa gaggaactcc gagaacttta tccgcaagct
     3241 aggattttgc gtatggatgt agataccact cgaagaaaag gggcacacga acagatttta
     3301 caaaaattcg gtgcaaaaga agcagatata ttattaggga cacaaatgat tgctaaaggt
     3361 ttggactttc cagaagtgac attagttggg gtgttgaatg ctgatacctc cctgaattta
     3421 ccggattttc gttctagtga gcatacattc caattgctta cccaagtttc aggacgagca
     3481 gggcgagcgg aaaaagcagg cgaagtcgtg atccaaacat tcaatcctca acattacgcc
     3541 atagaattag ctaaaaaaca aaattatgag cagttttatc agcaggaaat gcatgtacgg
     3601 catcgtggag gctatcctcc ttattacttt actgtgaaaa taaccgccag ccatcctgaa
     3661 gaacaagtag cggcaaagaa aatctttcag attgccaatc agctaaaaga agtgttaagc
     3721 cctcaaagct tgctattagg gccaacgccc agcatgattt tgagagtgaa aaaccgatat
     3781 tattatcaac taatcatcaa atataaacat gaaccgaatc ttcctcatgt tttggatgaa
     3841 attttgaatg gaagccagaa agaacagcga caaggtcttt ttgtggcaat tgacaacgaa
     3901 ccattaaact ttatttaaga aagggccaaa ttatgcgcta tcctatattg atccacccta
     3961 atgataaatt aaaaagaact gctcagccaa tcgatgtaat cacggacgag acaatcgctt
     4021 tattagacaa tttatatgag acaatgattg cgaatgacgg aatagggatc gctgctcctc
     4081 aagtaggaca aaacaagaga attgctgtga tcgaagtaga tgaaggcgag aaatttgaac
     4141 tgattaaccc agaaatcatc gaagcaaaag gtgaaagcct tgacgtagaa ggttgtctca
     4201 gtattccgca tgtctatgga acggtcaagc gagcagatga agtgaccgtg cgttattatg
     4261 atcgagacgg agaagagata gaagtgacgg cttttggcta tttagcccgt gccttccagc
     4321 atgaaatcga tcacttagac ggtatcttgt tcattgagaa aatgatacag cagatcccgg
     4381 aagaagaact tgaagaatat atggaggaac atcttgatga ctaagctagt atttatgggc
     4441 acacctgctt tttctgtgcc gattcttgaa gggctgatag aatcaggtta cgagatccag
     4501 gcagtggtaa cacagcccga ccgtccagtt gggcggaaac gagtaattac gccgacaccg
     4561 gtaaaagaag cggctctgaa gcacggtatc cgtgtgctgc agccagagaa aatctccggt
     4621 tcgccagaaa tggaagaaat cattgaattg gtgcctgatg tgatcgtcac tgcggcattt
     4681 ggccaattct tgcctgaaaa gttattgcaa gtaccaaaat taggggcaat caatgtccat
     4741 gcttctttac ttcctaaata tcggggaggt gcgcctgttc attatgccat catgaatggg
     4801 gagaaagaaa ctggtgtaac gatcatggaa atgatcaaaa aaatggatgc aggcggaatc
     4861 tatgcgcaag aaagcatacc aatcactaaa caagatgatg tagggacgat gtttgaaaag
     4921 cttagccttt taggaagaaa gctattgcta gaaacattgc ccaatatttt ggatggacaa
     4981 aaaccagtac ctcaagacga gtcagaagca actttttcgc caaacatcac acgggaacaa
     5041 gaagcaatcg attggcataa aactgcagaa gaaattgaca accaagtacg cggaatgcgt
     5101 ccttggccaa ttgcattcac gacctatgag ggcatacgct ggaaattatt atcggttgaa
     5161 gtattggcag aaactacaga caaagcacct ggtacgatca tccggaaaga caaaaaaaat
     5221 ctatggattg cttgcggtaa acagaccgtc ttagccatca atgaattaca gccagctggt
     5281 aaaggaaaac aggccgtaca tgatttcctt aacggaagcg gtcagcaagt tcagattggt
     5341 cagcaggtga aataatgaaa aaaatcccta aaaaaatgaa aaaatcagta cgatatatgg
     5401 cattactgac attagagcga gtgaacaagg gtggcgccta ctcgaatctt ttgttgaatg
     5461 agatgatcaa acaaagcgag ctaaatcaaa aagatgtggg tttatttaca gaattgatct
     5521 atggtacgat tagccatcag cgactattgg aattttacgt tgcaccattg atcgctaaag
     5581 caaaaaaagt ggatgactgg gtcaagacgc ttctttatct ttctgtatac caattagaat
     5641 ttttagataa agtccctgca catgctattt taaacgaggc ggtcgaaatc gcaaaagtaa
     5701 aaggaaatcc tgggacaggg aaatttgtaa atggtgtctt gaggaattat cagcgtcaag
     5761 gagcaccaga tctgcaaggc attgctgatc ctatcgaaag actttctgtc aaaatcagcc
     5821 tgcctctatg gatgacgaaa cgactggtgg atcaaatcgg atacgaagaa actgaaaagc
     5881 tcggtttatc gctatatcag cctagccatg ctagtgcccg agtagataca cgccgacttt
     5941 ccagagatca ggccatcact gttttgcaag aagaagagat cacagctgaa aaaagccaag
     6001 tcagtccata cggtatagta gcgaaaaaag ggcatttagc ttctagctca ttgttccacg
     6061 atggcgtcat gacgatccaa gatgaaagtt cgatgcttgt agcgccgtct atgcaaatcg
     6121 agcctgaaca tcatgtctta gatgcctgtg cagcacccgg aggtaagacg acgcatattg
     6181 cttctttttt agatccgtca tgcggtggag aagtggtcgc tttagatgta cacgaacaca
     6241 aagtcaaact cattgaagat aatgcagcac gtctaggtgt ctcttctgct gtctacgctc
     6301 aaaagatgga tgctagagaa gtcagtaaac agttcgaagc agagcagttt gatcggattt
     6361 tagtagacgc gccttgttca ggtcttgggc tgcttcggcg aaaaccagat attcgttata
     6421 aaaaacagcc ggatgaatta gcgaatttac caaaaatcca attggaaatt ttagaaagct
     6481 gcgcaccaac tttaaagtct tccggtattc tgacttatag cacttgtacg atcttaaaag
     6541 aagaaaacca agacgttgtt tctgctttct tggaaagaca ccctgatttt gagaggattg
     6601 atgttttgac caatcctatg ctacaatcat caatcgaaga gaaaatgttg actttgtatc
     6661 cgcatcagtt ttacacagat ggatttttca tttgttgttt gcgcaaaaag taatgaggtg
     6721 aaaaaatgca gattgaatat caatcagatg taggaagacg acgtaacaca aatcaagact
     6781 atgccagtgt atttaccagc cgttcaggtc tcaagcttgc tgtattagca gacgggatgg
     6841 gtggtcatcg tgccggagac attgccagtc agatggcggt aacgaatcta ggcgaagctt
     6901 gggaaaaaca agaagctgtt gacgatgaaa aaatcgccca atggttcatt caatcgattc
     6961 aagaagaaaa tatgctgatt tatcagcgtg gacaagaaca gccggaatat aacggtatgg
     7021 gaacaacaat cgtagcggcc gctctgtcag atgactggtt cacgattgcc aatgttgggg
     7081 atagcagagc ttatttgatt cgtgaagata aaatcatgca attgacggag gatcattctt
     7141 tagtcaatga actagtcaaa tcaggagaaa tcagcgaaga aatggcagtg aaccatccaa
     7201 ggaaaaatat tttaacacgt tctgtcggca tgcctggaac agtcgaagta gatgtttcta
     7261 ctcatctttg gcaactaaaa gatcgactgc tgttatgttc agatggcttg acgaacatgc
     7321 tgtctgaaga agaaatagga acgatcatca atcaagaagg cccattgtct cataaagtaa
     7381 ctgagctgat tgatcgtgcc aatgaagcag gcggagcgga taatatcacc gttcttctta
     7441 tcgaatataa ggaggacagt gtatgatcga gttgggcaaa aaattaaatg gccgttatca
     7501 aatcactgga aatatcggta gcggtggtat ggcaaatgta tttttggcgc acgatttaat
     7561 cttggatcgt gatgtagcag ttaaagtctt acgatttgac tttcaaaatg accagactgc
     7621 gattcgacga tttcagagag aagctttagc agcaacagaa ctcgttcatc caaacattgt
     7681 tagtgtttac gatgttggtg aagaagacaa catgcagtat ctggtgatgg aatacgtcaa
     7741 aggaatggac ttgaaacgat atatccaaac acattatcct gttccttatg aaacagcagt
     7801 aaatattatg cagcagatcc tttctgctat ttctttagct cataaccatc aaatcatcca
     7861 ccgagatttg aaaccccaaa acgtactgat cgataatgaa ggtgttgtaa aaatcacgga
     7921 tttcgggatt gctatcgctt tatctgaaac atcgatcacg cagacgaata cgatgctggg
     7981 atctgttcat tatctgtccc ctgagcaggc cagaggaagt atggcaacga agcagtcaga
     8041 tatttatgct ttagggatca tcctttatga gatgttgacg ggtagcgtac cgtttgatgg
     8101 tgaatctgct gtaacaattg ctttaaaaca ttttcaagat gatcttccat ctataaaagc
     8161 acttgatcca aatgtgccac aagcactgga aaacgtgatt ttacgggcaa cggctaaaga
     8221 acctgcagac cgttataagt cagccgagga aatgtctgat gatctttcta cagtattatc
     8281 accagctcgt gcaaacgaag aaaaatggca gcctcatgtg atggataatg aaacaaaagt
     8341 tatcacaccc cttacggaag atgcaccaat gcctgattca ttcaaatcta tgcctctgcc
     8401 aaaagacaag ccagaaaacg aaaccttgcc agaggaggaa gaagttctgc ctgatcctaa
     8461 aaagaaagga cagaaaaaat ggtggctgat cctattagct attcttgctg ttttaggaat
     8521 cggaacagct gtattctttg cttctggcgg gcgaggagaa gttgttgttc ctgatgtttc
     8581 tgatctttct gaagcttctg ctagagaaac actcgcaaaa gcgaaattag aagtagcaac
     8641 aaaaacagaa gaaattgcag atgacaaaat cgaagaaggt aatgtagtga agacagaccc
     8701 ggcagcggga acgacagtca agcaaaaacg cgaagtcact ctgtatatta gtagcggcac
     8761 gaaaaaaatc aaattagatg attacagtgg taaaagttat gaagatgccg aagcaagttt
     8821 gaaagaatta gggttttctg ctgacttgat tcaaaaagaa gaggaattca gttcagaggt
     8881 agaaagcgga atgatcattt cgcaatctcc atcagaagat acagaagtcg atcctaaaac
     8941 agacacgatt actttcacag tcagcaaagg accagaaaca gtgactttga gtagtcatgt
     9001 aggagaaaac tacgaaactg ttcgtaattg gcttgttaca caaggattca atatcaataa
     9061 tattagtgct acttatgact atagcgacac tgtaccatca ggttcgatca tcagtcagga
     9121 tccagcctcg ggtgaagttg tagcagaaga aacttacatc aattttgtcg taagccaagg
     9181 aacagagcct aaattgaatg atatctccgg ctatacaaaa agcgaagcac aaagttattt
     9241 agctagtgtc ggtgcagaat atatcgggca tgagacttcc gaatattcaa acacggtgga
     9301 taaagataaa gtgattcgta cagtaccagg ggcaggaacg acaattacca aaggaatggt
     9361 tgtgaacgta atttactcta aaggaccaga tccagcgtct tctagttctg aaaaagactc
     9421 ttcagacagt tctagcagct caagtagctc cagtagcaca agcaactctt caaaagaaga
     9481 atcttccagc tccacaagtt catcagctag cacctcagaa agttcaaaat aagtgctgaa
     9541 taaaatatat cagaagggaa tgcgacattg atttgttgca ttctcttttt tgtgtttcta
     9601 tctcttttga tcaattttca agccatatac tttgagaagt ttgcatggaa taacacctac
     9661 tttcctcttc gtatggtaaa ataaacaaaa acggaggtgg cacttatcaa aggacaaatc
     9721 agaaaagcaa tcagcggttt ttactatgta tacgcaaacg gagagaccta tcaaacaaga
     9781 ggaagaggaa atttccggaa tcggaaaatc acgccgcttg tcggagatca ggtgatcttt
     9841 gagagtgaaa atcaaacaga cggctatctt ctggaaatct tgccgagaaa aaatcaatta
     9901 gttcgcccac cagtcacaaa tgttgatctt ggaatcattg tcacaagtat cgtagagcct
     9961 gcattttctt ataatctact tgaccgcttt ctagttacct tggaatacga gtcgatcgaa
    10021 ccgattattt ttttgacaaa gaccgacttg tcagacgacc cagaaaaaat cgaagagatc
    10081 aagcaaacct atactaaaat aggctatccg gtgattgtac ccaatcatac gggtgatacg
    10141 acagaactga tccgttactt cccagaacgg ctatcggtct ttatgggtca gtctggagca
    10201 ggaaaatcga cgctactgaa tcaaatatca ccggaactga atttagcaac aggagagatt
    10261 tctgatgcac ttggaagagg acggcataca acacgtcatg tggaactgat tcctatctat
    10321 ggcgggttag tagcagatac accaggattt agctcgattg actttttgac aattgaagca
    10381 ggagaactgc cgagacaatt tcctgaattt gtagaagctt ctgtccattg caaatttaga
    10441 gagtgcatgc atgctaaaga acctggctgc gaagtaaaac atagagtaga aacaggtggt
    10501 atcgcacaga cacgttacga caattatttg caattcttac aagaaatcga aaaaagaaaa
    10561 ccaatgtatg caaaaaaaga aggtaaacga aaataggagg aaacaagaat gaagatagca
    10621 ccatccattt tgagtgcaga ttttgccaat ttacaaagag acattgagtt agtggaaaaa
    10681 ggaggcgcag attacatcca cgtagatgta atggatggcc aatttgtccc aaatatcaca
    10741 ttagggccaa atattgttca ggcaatccgt cctgttacta aattaccgtt agacgtccat
    10801 ctgatgatcg ttgatccaga aaactacatt ccagcatttg caaaagctgg agcagatatc
    10861 attactgtcc acgtcgaagc aactcctcat attcatcggg cacttcaaat gacaaaaagc
    10921 ttaggagtta aagctggagt agttatcaat ccaggaacac cagtctccat gatcaaacat
    10981 gttttgcctt tggctgatca agtactagtc atgactgtca atcctgggtt cggcggacaa
    11041 agctttattg aagaaacagt agaaaaaatt acggaactcg ctgatctacg tagagagaac
    11101 aattggactt atgcaatcga agtagatggc ggaatcgttc cagaaacagc aaaaatctgt
    11161 aaagatgcag gggcggatgt cttcgttgca ggatcatata tctatggagc cgaaaatcca
    11221 gtcggacaaa tcgaacgatt aaaagaggca ttagactaaa tgaaaatttt attagctgct
    11281 ggaggtaatc cgcaaaactg gccgcagtct ctgctgtcca aatacgatgc ctatgtcggg
    11341 atagaccgtg gtgcgctgta cctgcttcaa gcaggctacc cagtagatct ggcagtgggc
    11401 gattttgatt cattgtcgaa cgaagagcag aaggaagtat ttgcaaaagc caaagagtat
    11461 acccgttccc ctgcagaaaa agacgataca gatacacagt taggtatctt aaaaacattt
    11521 gaacgttacc cgcaagcgga agtcactttg attggagcta cgggcggaag attggatcat
    11581 cttttagcca atctctggtt aggtatggaa cctcgcttca aaccctttgc ccatcagctt
    11641 attatcagag attgtcaaaa ttatttgtct tattatttgc ctggagagca tatagtaaga
    11701 cagataccag atatgaagta tttagcttat tgctgcttga ctcccgtctc gaatcttaca
    11761 ttaaaaggca gcaagtatgt attggaaaat aaagaagtag aatatccgac atcttatgca
    11821 agtaacgaat ttctttcaga taccgcagaa ttttcttttt cagatggtat gattgcagtt
    11881 attcaaagca aagattgatc cataaacaaa taaaaaaatt tgtctttcag gctaaaaatg
    11941 acagaatcta ttatttgcta aaaaaaagct taagaggagc aacttacctt ttgtttcacg
    12001 gcatttagtg ataaaattga acacaagagt ctaattaatt cgaggtgtct ttgtgaagaa
    12061 gttcaatcct aataaaaata ttattattac cttggttatt gttatcatcg tggtgacgat
    12121 actaagtgtg accgctgcgc agcgagcgaa tgaaggaaag cccaatttct tccaatccat
    12181 cgttaatgac agtgttgcaa tggttgataa aacaatctct gccccagtac ggtgggtacg
    12241 aaatggagta gattctgtcc ataatctttt ttcaacttat gccgaaaatg agcggctaaa
    12301 agaaaaaatc gatagctatg atgacgtttc acagcaaaac aagaattacg agagagaaat
    12361 cgaagcgttg aaaaaagaac ttgagttgaa tcagacgctg actagttacg aaaaagtaac
    12421 ggcaaatgtc attactcgtt cgcctgacag ttggcaggac atgctgatcg tagacaaggg
    12481 ttctaaagac ggtatcgaag caaatatggc agtcatgtcg caaaaaggat tgattggccg
    12541 agtgatcgag gtcaatacgg cttcgtctaa aatcgaatta ctgtcatcct ctaatgaaag
    12601 ctccaatcat tttccagtac gggtatcttc ggctaatggc gaagcgtttg gtttgcttaa
    12661 aaactatgat gaaaagctcc atgccttagt ggtgacccaa ttaactggtg atacggatat
    12721 caaagaaggg gatgttgtcc agacatccgg tcttggaggg aattctccag ctaacttgcc
    12781 gatcggtacg gttattaaaa cgaaaccaga tagttatggg ctggatcggg aagtttatgt
    12841 gaaaccttat gcagaaatgt atgacgtgtc agttgtgacg attgtccaac ggctagcgga
    12901 ggattagtcc atgttgaaga aagaaacaat gaagtactat ctgccaatcg ttttgttctt
    12961 tttgatgttg atagatggtc atttaacaag aatgctaggg gagtggtcga aaggcaccta
    13021 tatgtcaaat gcccactttc tgatattggc attattatgt tgcagtatgg cgtttgaaaa
    13081 acgttattta ctgattacca cgattgttct cggggctatc tatgacgctt actatattgg
    13141 cgttatcggt atctatgcag tagctctccc tttaattgta tggttgatgt atgtaatgaa
    13201 agacgttatc catgtcaaca tctttactga atttttcagt atgatcatct ttgtcacggg
    13261 ttatgaattg tttacgatgg tggtccagtt gatttttaaa ttagcagtag taaataacac
    13321 gtattttatt acaaggtttt taggacctac actgctgttg aacatgatta tatttgtatt
    13381 attcattttt ccctttaaga aat
//
LOCUS       DANKHL010000069        13399 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000069 DANKHL010000000
VERSION     DANKHL010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.072"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            4..2376
                     /locus_tag="PW101_001412"
     CDS             4..2376
                     /locus_tag="PW101_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513816.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="PW101_001413"
     CDS             2437..3897
                     /locus_tag="PW101_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDL2513817.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="PW101_001414"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="PW101_001414"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2513818.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6128..6277
                     /locus_tag="PW101_001415"
     CDS             6128..6277
                     /locus_tag="PW101_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513819.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6352..6945
                     /locus_tag="PW101_001416"
     CDS             6352..6945
                     /locus_tag="PW101_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDL2513820.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6958..7857
                     /locus_tag="PW101_001417"
     CDS             6958..7857
                     /locus_tag="PW101_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDL2513821.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7860..8357
                     /gene="ssb"
                     /locus_tag="PW101_001418"
     CDS             7860..8357
                     /gene="ssb"
                     /locus_tag="PW101_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL2513822.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8723..8983
                     /locus_tag="PW101_001419"
     CDS             8723..8983
                     /locus_tag="PW101_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513823.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9428..9634
                     /locus_tag="PW101_001420"
     CDS             9428..9634
                     /locus_tag="PW101_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HDL2513824.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9682..9885
                     /locus_tag="PW101_001421"
     CDS             9682..9885
                     /locus_tag="PW101_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513825.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYENEYWLTKLEN"
     gene            9901..10338
                     /locus_tag="PW101_001422"
     CDS             9901..10338
                     /locus_tag="PW101_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513826.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10331..11038
                     /locus_tag="PW101_001423"
     CDS             10331..11038
                     /locus_tag="PW101_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513827.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11418..11597
                     /locus_tag="PW101_001424"
     CDS             11418..11597
                     /locus_tag="PW101_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513828.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11922..12185
                     /locus_tag="PW101_001425"
     CDS             11922..12185
                     /locus_tag="PW101_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HDL2513829.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12179..12529
                     /locus_tag="PW101_001426"
     CDS             12179..12529
                     /locus_tag="PW101_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2513830.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12553..13167)
                     /locus_tag="PW101_001427"
     CDS             complement(12553..13167)
                     /locus_tag="PW101_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2513831.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         4961 a         2206 c         2746 g         3486 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaagaatac
      241 gtggaaaagc ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgg ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctaga
      661 ccaactgatt ctatgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacattgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaggaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaaccaat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaagggt tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctatacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tttagaaacg ccagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagatta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgaaacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaata tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagataaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagtcgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aaatcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtaa atgcgttttt tggccagcag ctttctgtta acgattcaat agaagccatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagtttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc gcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg agatccagca acaagaaatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggcagac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccactc gaaaagacgg gtattttgaa atccaagatc gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gaattagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agaacatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagtag gcgccgatca aggaaaaacc tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagacg caaaagagac
     4381 ctatccaaaa tttgaagaag cacaagcacg tcagatcgcc gattggttaa ttgggatgaa
     4441 cggtagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gcttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttgcaggaaa agattagaaa
     4561 tttcaagaaa gaaccttatt ttgaaggaaa atccgaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggcatg attcgccaag ttgaaaccga
     4741 gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagacac tttggaagcc atgcaaggct tgtatgaagg
     4861 gaaatatcta agctatccgc gaacggatac cccttacatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttgagg gctgaagaaa tttcgactcc
     4981 tattcataca ccgaatagta ggtacgtgaa caacaagaaa gtccaggaac attacgcaat
     5041 cattcctaca aaaacagtta tgacagaagc gacgtatgaa agtctgacac ctttacaaca
     5101 agcgatttac gagcaagttt taaaaacgac agtggcgatg tttgcggaaa aatataccta
     5161 tgaagaaaca acgatcttga cgcaagtcca gcagcttcag ttaaaggcaa tcggaaaagt
     5221 accgatagat ctaggttgga agaagttatt tggcaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcgat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agcttgtggt gacgaataaa ggggttttgc tttgccaagc
     5581 tgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatt ggagaacgaa aaggaacaca aaccactttt ctagccaata tccaaaagtt
     5701 tgtctctcac ttattggaag tcgtcccagg acaaatccaa tccattgatt ttggatcgac
     5761 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
     5821 tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga
     5881 agcctgtgat tttaaattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggagatt attcaaaata gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa
     6001 gggttcgttt gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc
     6061 tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc acgacaaaat aatggagggt
     6121 taatcttatg cgtaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa tcgaattaga tcgatagaca ttaaccttgt tacaaataca
     6301 ctatcatgat atatttaatg tagtgtattt gtaacaaaag gggggaagta aatgcaagag
     6361 aaactgaaag aattgttggc tattcgcgat ggaaatttat ctatgaagga agccagaaaa
     6421 ttaggaatcg cagcaaccac tgtacaaaga ttggtgaatc aaggaaaatt gatcaaagtt
     6481 gatcgtggtt attatgtaga ggatggtcat ggaatagatg atttattttg gcttcagcaa
     6541 cgtttttcca ggggaatctt ctctcatgaa acgactttag atatttacca gctttctacg
     6601 aatatgccga aattcattca cttaactttt ccccatggat ataacgttga tcgttcaatt
     6661 acaaaagagc agcagttaca atttcatttc gtgaagaaag aagtttatga actaggaaaa
     6721 gtagagggaa cttcctttca aggaaatcct attactatgt atgataaaga gcgtacactt
     6781 tgtgatattt ggaatccgag atatgaaatt gaatatgaaa tgaaattaga agcattaaaa
     6841 gagtacatgg aagacccatt gagagatcca agtaaattaa gagattacat gacaaaacta
     6901 catgtagaaa aagaaatgaa ataccacatg caaagcttat attgatggag gaaaaagatg
     6961 acaccgacac aatttgcagc aaaaacaaga aatttagcaa agaggacagg attaaatgca
     7021 cagttagtac aacggcattt tatgatggat cgtttaattg aacaaatttc tgaatctaag
     7081 tataaaaatg atttcatttt gaaaggtggc tttttattag gatcaaaata tggaatcgat
     7141 agaaggacga caattgatat cgacacgaca tttagaaata gcaaattaac tgaagaaaaa
     7201 ttgaagagta tttttaatga tttaactagg aaacccacga aagaaggtat tgttttctct
     7261 attcaaggaa tgaaggaaac aagagaagcc gatttttatc ctggatttca aattaaattg
     7321 gtggctaatt tagagaaagt acgtgtgcca tttaaaatgg acgtgacaac aggagattct
     7381 atttatcctg aagtagatac acactatcac caattaatgt ttgaagataa aaaggtggag
     7441 attccagctt atccaacagc acaaattatt gcagaaaagc taagtgctac gttcagcttt
     7501 ggcacagata attcgagagc aaaagattac tacgacttat ttaccattcc gaaactagaa
     7561 aatatcgata cgaaagagtt atacaaaagt gttcgaaata catttacaaa acgtggggat
     7621 acaaaaccgt tgtcctttta ttatgaaaga gaaatggata agatcatagg aaatgactat
     7681 ttggaaaagc agtgggaaaa atatcgctca gttaacacgt ttgcgaattc catttcattt
     7741 acagatacag tcaacgaaat tgatcaccta atgaaaagtg tgatcaaaat tgaaaatcaa
     7801 gaaagaaaac ggcagtttaa aagatcacaa caaatgaatc aagaaatgga gagataaaga
     7861 tgatcaataa tgtaacttta gtaggacgct taaccaaaga tccagattta cgttataccg
     7921 cgagtggtac tgcagtagcg acttttaccc tagctgtcaa ccgaaatttc acgaatcaaa
     7981 acggcaatcg agaagctgat tttattaact gtgtgatttg gcgcaaacca gcagaaacaa
     8041 tggtgacatt agctaaaaaa ggtagcttga ttggtgtagt tgggcggatt caaacgcgga
     8101 cttatgacaa ccagcaagga caacgtgtgt atgtgacaga agttgtggcc gataatttcc
     8161 aactattaga aagcaaagca gcgacagaaa gccgtgcgca cgctgatcaa tcgtccacca
     8221 gtccaagcac caccactttt gaacaacggg atacagcgac gcctaataac aatggattga
     8281 acgctttaca gaatccattt gggaatccat ttggtggaca atcaatcgat attagcgatg
     8341 atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt ttatgttata
     8401 atttagtcaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg gcgggctagt
     8461 ggttttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc tttcttggca
     8521 aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat gattgcaaaa
     8581 acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat taggccgggg
     8641 tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg atttttgcta
     8701 taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata aaatccgagt
     8761 aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg aatcgattaa
     8821 ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg atggaaaata
     8881 caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg ctagcatgta
     8941 tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaaaaaaga agagttgcaa
     9001 tcctaatgta ggactgcagc tctttttgtt ttacatagaa acaagaaaaa tgaaaaggcc
     9061 tttttaggaa ggcgcgagcg ccttctcaat tgaaaaacct ctctcactcc aaccgccacc
     9121 gccgttcgag aagaaaggaa gtgaacgttg cacgttcact aagataaagg gtcttgcaaa
     9181 gcaagacaaa atagaaaaaa cttcctctca ccaaaaggtt cgttcctgtt cgttatctct
     9241 ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg acaaggacaa
     9301 gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattc attctggcca
     9361 acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag aggaggcaga
     9421 atcaatcatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat tattcccaaa
     9481 ctatcatgca ccacatactg tcgaatgcga ttgtggacaa agagcgaaat gtatcaaaag
     9541 ctatcgtttg tatcaatgtc caacatgcgg aaaaaaatac accttgtctt atggcgatta
     9601 tattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cataacataa attattgact
     9661 gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa agtagcagaa
     9721 gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga tgcaccgact
     9781 atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca agaaaacatg
     9841 gaaacttatg aaaacgaata ttggttaacc aaattagaga actaaaaagg agaaaaaaat
     9901 atggctcaaa attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa
     9961 atggaggata tgacattcct ataccatgag acacgtgttc caaaaaaaca ttataaggaa
    10021 agattgtcgg tagctgtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg
    10081 acgtactatc gaacactgga agaactgaaa aaacaaaatg caaaatggtt tttccaagcc
    10141 ttactatgct tggaagtagg tgtcaaacca agcactatta aaccttctga atatcaagct
    10201 ttagaactaa cgtatgggaa atttgtcgaa acgaaaaaag ccaagactgt ttcaagtgaa
    10261 tggttagatt attttgaaaa tatcaataag tatggcgcct attacaccat gaaaaaggag
    10321 gacaacgaaa atgaataagg tgatttatca actaacaacg gaagatgtgt cacacttttt
    10381 aacaaacaaa gaacatttga atgaagtaat cagacggatt caaaaagact ttagttgtat
    10441 ggaagaaatg aagtacttta ttcaaagagt cgaagaacaa ttgtatttgg aaagtgaaac
    10501 gagtcaaacg aaggtcacag tcagcaataa ttatttagac gtgtataaac atcgtcctga
    10561 aacggtaaaa aaaggaacgg agcttctacg actagaaaca gaaaaataca tcatgaatgt
    10621 ttacctagaa aggagtgaac tggtcgaaaa ctttgtcagc tgcacgattt gcaggaaaca
    10681 ctattactca gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga
    10741 aatggaagag ctaaacagca aaaatgcgac gatagacgat tattatcttg tatttactag
    10801 atttttgacg tgtggaacgg aatctatcgt gttaatggga atggattatg aactcgctgt
    10861 acccgaagca tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta
    10921 cacgacttta actgattttt tagataccta cacttacgac gatacagaag aaatccaaaa
    10981 tgaacttgac cgtaaacgaa tgccatatca acgatttgaa aggaaaaaaa ggcaataatc
    11041 atttataccg aaaagtgaga atgtgtgaaa taagaacaaa aaccttgaaa ttaagggaat
    11101 ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt gcgaaataag
    11161 cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa atcattcctc
    11221 tgttgttcgt actgtgtatt ggcgagatac ttatgcgtca tggtgcagct gtacatttaa
    11281 acttcgtagt attcttagga atactagtat tttgtttgat tttttggcgc tttgtgttag
    11341 cccttgtgag tctactattc aagttgattg cagccatatt tttcataggg ttatttcgat
    11401 gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc gtgacattag
    11461 atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa agtgaatcta
    11521 agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga tttgatatac
    11581 cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata gcacttaatt
    11641 gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat caataactat
    11701 gaaatcttta aagtatccag atcaattttt ttcttacctg attctaaaga ttttgttttc
    11761 ttaaacatat tatgatttcc tttcgactat tttcatataa atgttaacat agtttatgga
    11821 ttcatggaat gttaatctaa tttaatagaa aaggataatt aagtatatac gttttttctt
    11881 atgtatgtta tactatgtat atacagaaag aggtgtctac tatggaaatg ttacctataa
    11941 aaaaatgggg aaatagtaat ggccttagat tgccaaagta tattatggaa tatcttggaa
    12001 ttcatacaga agacaaagtg aagataatac aagaagaagt aaacggtcag aagcggctaa
    12061 ttatcgaagc ggctaatata gaaaatgaat taacaattga acagttattt gaaaattaca
    12121 ctgatgaaag aaatcatgtg acgattcaaa atttaggtga agcagtaggt aatgaaaaat
    12181 ggtgagaaaa cctagacaag gcgatattct attattgaat acagcacctc gatctggaca
    12241 cgaacagaca ggtaaaagac cttatattgt tttaagtcat gatattattg cggattatag
    12301 taacgttgtt acagttgctc caatttctag tacaactaga aactatccgc tatatgtaaa
    12361 tataaactca gaatatgaaa tgaagacaac aggaaaagta ttattagatc aactaacgac
    12421 aattgattat gaagccagag agtgtatttt tttggaaaaa gcgcatgata gtttagttga
    12481 ggagttattg atgaaagtta gaacagtttt tcaaaaagta agcaaataaa atagaaacag
    12541 cagcatgatc aactattcgt gattgaccat gctgtctttt tttattctat atacggtttg
    12601 tctcgtaaca ttgtagtctt ttgcaatctt agcgatcgca acaccttttt ttagatcctc
    12661 aacgatgttt ttataaacaa gacgacgttg gggatctttt gcattggcac tgtatagctt
    12721 aggacgtcct ttataaacac cgttcgcttt tgcaatctta atgccttgtg cctgacgtcg
    12781 tttagattcg gtacgttctt gttcagcgat catggccaaa atttgaatga tcaaatcttt
    12841 aatgaattta tccaggagag gattgccaat tgcttcggcc atgatcggta gacttgtgat
    12901 gatcagctgc acatttttct tcttcaagta attgactgaa gcgatgatct cgtcgtaatt
    12961 tcttccaagt cgatcaatag cttccacaat aaaaatatct tgatctcgga cgaaatccag
    13021 tgcttcttga aaaacaggtc gatgagtaat cgttgcgcca gattgtttct caatgaaaat
    13081 tctctctgca ccgaattttt tcatttcttc aatctgccgt tgttcatttt gatcgatgga
    13141 tgagacacgt atgtatgcaa ctctcaaaat ttttacctac tttctgcaac tatttaaaaa
    13201 acaccctaat catacacgag aaaaccagat ttttcaatgt cgcggtcgtg tctagttagg
    13261 gtatacctta tatagacaaa aactaatgat ggaggaaata gtatgtgtca cttttctttt
    13321 taaatggcaa tattttttgt aaataaatgt caatcaagaa aatttaaacg attatgacat
    13381 taaaaatata ttttatgaa
//
LOCUS       DANKHL010000070        13296 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000070 DANKHL010000000
VERSION     DANKHL010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.073"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..113
                     /locus_tag="PW101_001428"
     CDS             <1..113
                     /locus_tag="PW101_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HDL2513832.1"
                     /translation="AGLVASVKKTVIDPIDEKEAMIEENRKKAMRKRVSR"
     gene            complement(175..1986)
                     /locus_tag="PW101_001429"
     CDS             complement(175..1986)
                     /locus_tag="PW101_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDL2513833.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(1983..2348)
                     /locus_tag="PW101_001430"
     CDS             complement(1983..2348)
                     /locus_tag="PW101_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HDL2513834.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2511..2906)
                     /locus_tag="PW101_001431"
     CDS             complement(2511..2906)
                     /locus_tag="PW101_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2513835.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2924..3886)
                     /locus_tag="PW101_001432"
     CDS             complement(2924..3886)
                     /locus_tag="PW101_001432"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDL2513836.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3886..4098)
                     /locus_tag="PW101_001433"
     CDS             complement(3886..4098)
                     /locus_tag="PW101_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDL2513837.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4119..4817)
                     /locus_tag="PW101_001434"
     CDS             complement(4119..4817)
                     /locus_tag="PW101_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDL2513838.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4837..5379)
                     /locus_tag="PW101_001435"
     CDS             complement(4837..5379)
                     /locus_tag="PW101_001435"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDL2513839.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5511..6515)
                     /locus_tag="PW101_001436"
     CDS             complement(5511..6515)
                     /locus_tag="PW101_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL2513840.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6512..7501)
                     /locus_tag="PW101_001437"
     CDS             complement(6512..7501)
                     /locus_tag="PW101_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL2513841.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7498..8304)
                     /locus_tag="PW101_001438"
     CDS             complement(7498..8304)
                     /locus_tag="PW101_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2513842.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8470..9426
                     /locus_tag="PW101_001439"
     CDS             8470..9426
                     /locus_tag="PW101_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2513843.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9503..10021)
                     /locus_tag="PW101_001440"
     CDS             complement(9503..10021)
                     /locus_tag="PW101_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2513844.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10109..10258)
                     /gene="rpmG"
                     /locus_tag="PW101_001441"
     CDS             complement(10109..10258)
                     /gene="rpmG"
                     /locus_tag="PW101_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL2513845.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10486..10932
                     /locus_tag="PW101_001442"
     CDS             10486..10932
                     /locus_tag="PW101_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDL2513846.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11127..13022)
                     /gene="asnB"
                     /locus_tag="PW101_001443"
     CDS             complement(11127..13022)
                     /gene="asnB"
                     /locus_tag="PW101_001443"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDL2513847.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4139 a         2822 c         2263 g         4072 t
ORIGIN      
        1 ttgctggttt ggttgcttct gtgaaaaaaa cagtgatcga tccaattgac gaaaaagaag
       61 caatgatcga agaaaaccgt aaaaaagcaa tgcgtaaacg cgtttctcgt taataattat
      121 ttaaaaatac gcatgaagac tttctaaaaa acagataagt catcatgcgt atttttattt
      181 gtcagtcaat ataagttgtg ctattttatc tgcctgtctc agattccatg gatctgtata
      241 aggtctagct actgctgcag ttagccaatc cacaccttca acaggtattc catagcaata
      301 aaagtcagac tggatttccc ctgaagcatc aatcacgcga ttcgtacctc gctcaacatc
      361 tatagcttct gaatgataac ttcctgtatt atctgaaaat gaaaaagggc gaacaagttt
      421 gttttccttc aaatttttta tgacaggatt acttgaacga gaaaagcttg tttgtggcaa
      481 tcgagcttca atcaaatagc gactgctgat ttctttatct tgtagagaaa taacatagcg
      541 gttgtgcttt tgctgaatat caaatggcgg ttcgatcaaa tgaaaaatac ccgcatctat
      601 cagtgctgct aattctttcg tccgaatgat tggcggaccg atcgttaaaa agctatttag
      661 atgagtaaac catccccata gtatttcttt gtattcctta gcagaaaaaa cgtgccattc
      721 aatagcctgc cggattggat cacgccagtc ttttaatgca tcaaatgttg aagcaaatgc
      781 tcctgtgcaa ttgccttttt ccgcttcttt tatctgccct tcaataaata ttcggctagc
      841 ttctgaaaag ggctcattct gataggcaag aggagtttct aaatacgacc aatcccatct
      901 ataagggatt agtgctggat gctttccgag acactcctct gaagagaggg agagaaaatc
      961 atgctcaaaa tttttctttg gaatcggcaa gtgatattct tcgatgattt tcttgtaata
     1021 aaacaactca acgtcttttt tcagatagtc aaaaaatgtt tcgccagtca aaaatcccga
     1081 ctgatgccat tgctccaggt tttcttttgt tagtaaccgt ggccatgcca ttgctccgcc
     1141 ttgcttttga tttcttcctc gagggtaata agggagcccc cttcttgatc cactgtatac
     1201 aatcggttcc ttgcctgatg gatgatagac cagttttccc ttttcgttct cgaagcgccc
     1261 tcctcttgat actgtaaaca atccgacata atcaaaaaaa cttaagccta gacctcttaa
     1321 aaagacattt tctttaggcg gtatcttcga aagatctaca tctgcaggat tagaaggaag
     1381 ctgatacagt aaaacatttt tctttgcgta gtctacccac actctttctt cttctgtctc
     1441 atcattttcc cagtgaccgc ttgctaaaac aagctgatcg atagaatagc tttgctgatc
     1501 tgtctgtaaa agataaccat cttttctttt gcttactttt tttactaaag aataaacaaa
     1561 ctgaatagaa gctggaaatt cttcttgcaa ctttgagaaa aaccagcgtt gatagagtcc
     1621 gtataaacat cgagtagatt gctgatcttt ttccagacga ttgatttctt ctataattag
     1681 ttgcttattt ttggttaccg tctgttcgat ataatctgta gcaggtcctt tactccattg
     1741 aaacaagttg ggtccctgtg ttaccaatcc tccactggac aatgtttcat ctgtaaaaag
     1801 cgtcacttgt tgagaaacag aattcattaa taactccggt ggctgattca accgccaaac
     1861 tcgacctcca ggaccattgg gatcgaaaag aaaaagttca agagaatgtc tgtcctgatt
     1921 attatggaga atcctttctg ctacacttaa tcctctaggt ccggcaccaa tgatccctat
     1981 tttcatttgc atgcctccat ttcgataaat tttttcaata aaacagcatg gttctgatgt
     2041 tcattttttg caccataaac aaaagtaatg actggatgtt ctttcataag ttgactgagt
     2101 ttaaaaatag cttgcttctt ttcttcttcg aaaatcaatt cttgctggta tttctttgca
     2161 aatgaagcaa atcgttctgg tacatggtca aaagttttgc gtgcttcatt actaggtgca
     2221 atttctttca accacagatc taactgcgct ttttcttttg aaattcctcg tggccagaat
     2281 ctatccacga gtattctata gccatcctct ttttcagcat caaggtaaac acgttttaat
     2341 ttgatcatag ttgtttcctt ctttctacct tttcgcttag cttatatatg tagataatag
     2401 cacaaaataa aaaaaatgaa cggcacaact atccattgcg ccgttcatgt gatcttggtc
     2461 aaaatctaat gattgattcg gtttttaaag ctgtcataca atcatctgtt ttatactttt
     2521 ttacttttga tttttccaga ccatccgtca tatccaccct taagtaaata aatctcttga
     2581 taacctgctt tacgtaattt atttgctgta cggatgctca aacttttctt ttgatcgtaa
     2641 atatagacag gctggtcttt tcgaatagat cctaatgaat ctttcaaaac agtgtaaggg
     2701 atgtttcttg cacctaaaat atgcccagca tcaaacacat ctttttcgcg aacatcgatt
     2761 acttgtgctt ttctcattcc ttctcgaaat tcttcttctg tcaaagtcgt tgctgaacgt
     2821 ttggccatta ctcgtagata gacttcccaa aggatgattg caagcacaat caatagcaaa
     2881 atgatattaa ttacccataa aaccatgtaa tgcctccgtt tttttaagca aatttcaagg
     2941 ctaatgaggc agcaccaatg acaccagctt cattaccaag ctctgctaat ttgatttttg
     3001 ttgattctct tacttgcggg aaagtaaatt ctttaaaata tttctctaca cggctacgta
     3061 aaaattctcc tgctgccgat actcctccac ctaacacgat acttgacgga tttaatgtat
     3121 tccccaagtt tccgcaagct aaacctaagt agaagcatac gcgatcaaca accatcaatg
     3181 caaacggatc gtcttcttga gccatttcaa agatatcttt acttgttact tcttctccgt
     3241 tgtctagcat tgctttcagt tttgaatccc ctgcgtattc ttctgccaag aaacgaccta
     3301 ggcgtaccac acctgtagcg ctagatacag tttctaaaca tccgcgttta ccgcaagtac
     3361 attcgaaccc ttcaggatcc acagtgatat gtccgatttc tcctccagct ccgctcacgc
     3421 cgtgaatcaa atttccgcca gcaacgatac ctccgccaac acctgttcct agagtaacga
     3481 atacaacgtc tggatcgttt tctccagcac ctttccatcg ttcacctaaa gcagcaacat
     3541 tcgcgtcatt atcgatagcg aatttgattc ctgttcctgc ctcgatcagt tttttcacaa
     3601 attgaacttc tgtccagttt aagttatacg caccaataac cgttccagct tcgctgtcca
     3661 cgcttcctgg ggtacccatg ccgattccga taaagtcttc ggctttcata ttatataaat
     3721 tcaaacgatg attgattgat tcaatgattt caggaacgat atgcttacca tcatccaaaa
     3781 tattggtatc gatgctccat ttttgctgaa tctctccttc tggtgttaaa attgcaaatt
     3841 ttgctgttgt tccacctaaa tcaatcccaa taatcttctt gtccattaca aatttccttt
     3901 ctttcggctt tcttcaattc ggtgctctct ttttaatatg cgccacgctg tcatgtatgt
     3961 atcatgatca atcaaacggc tatcatacag attacgcaat tcgatagtca ttaattcaat
     4021 gtcatagatg cgtgcaccca catagataaa gatgccaaat tttttgaaca gctgctgtac
     4081 atcatataag ctctccatct ttaagcactt ccttcatatt atacaagtaa accatatttt
     4141 tttaaaccca gtaaaataca tattacaaaa agaaatacaa aaactagtgt agaaagaatc
     4201 ctttggtgta tcgaatagct tgtctgttgt ttcggtagac ccaaaacatt ccctaacact
     4261 aatccgccaa ttagcccacc gacatgtccc caaacatcta cagaggtatc cagaagacca
     4321 aaaacaaatg tcaacacaat aaataaaaga aaatgtctga ctagctgctg gatcgctggg
     4381 ttatgtttaa aatgaaaccc taagataaac aacgcaccga acaaaccgaa tatcgaagta
     4441 cttgcaccac cagacagaac attcgcttcg ttaaaagcaa agcttgctgt atttcccatt
     4501 atcccgctga acatataaat caagaaaaaa cgccaatggc catagattgc ctcgatttgc
     4561 tgccccataa aatacagtat cagtgagttc attacgaaat gcatcagtcc aaaatggatg
     4621 aagattggtg tcactaaacg ccaccattca ttgaagtgaa caagataagg accatacatt
     4681 ccggcataat aagggatctc aagtcgcgga aataattcca tcatgaggaa gaccgctgtc
     4741 tgtatcccta aaaaagcata ggtccataac ggtcgtctta accatgcacg caattttatt
     4801 tgctgttgat aattcatgaa aacctcccat ttcattttag tcaataaaca attgttggat
     4861 cttctgatca aagctctccg gttgccattg ttcattcaat tgttcacgaa aaacaaggct
     4921 acatgttttt ttatgaaaat tatttaaata ccggtcgtaa taaccaccgc caaaaccgat
     4981 tcgataaccg gctctattgt agataatacc tggtacaatg aggagatcga tcatctccgg
     5041 caaatagagc ttctctgatt gtggctcaag aacaccaaaa ctcgaacgta caagaacgga
     5101 ctgttcatcc atttcataaa acgccatctg acgtttcggc aaagttttag gtacaactat
     5161 tttcttttgt tctttttctg ctcgttggat aaccggctgt gtatccagtt caaattcatt
     5221 cgataaagtc gtccctatca cttttgctga cttccagtac tcgctggcga atagctgttt
     5281 atagattcgc tgttcttttt cctttttctt gccacttttg gccagatttt tcagcttttc
     5341 gattcctttc cggcgtaatc ttactttcaa ttggtccaaa aactcaccct tccttctctc
     5401 ataaaatggt ataggaaaaa aagaaaaata gcaatcgaac tttcaaataa atgcataaat
     5461 agaataaaaa gaagacctag aaatgattgg ccttcctttt ttaatgaatt tcaactgttc
     5521 agcgcgtgtt tttgtctgat aaaaagtgca tacagaaaat atggcgcacc aaaaagggca
     5581 atcaaaattc ctgtaggtac ttccccactt ggtagaatca ctctagcaac catatctgca
     5641 ctaagcagaa aaatggaacc gaacaaaccg ctcagaacaa aggtccagtt attttcttta
     5701 cgtacgacta gcttcgcagc atgaggagct agtagtccta caaaagataa actacctgtc
     5761 accgctacac taccgcttgc caaagcaaca gctaacaaca aaaggatttt ttgactttta
     5821 gccacgttaa ggcctaatcc ttgcgcgcgt tcttgaccaa atgatagcaa tacaatcctc
     5881 tgtgcataaa gtcctcctag cggaattaag ataaccgccc atggaagcaa tgcccaaaca
     5941 taaggccagc tcgcacccca gattgatccc gctaaccatg cattcacaaa tccataattt
     6001 tccttagata attggatcgt gcatagcaat gttaatgcag aaaggccggc attaacggca
     6061 ataccggaca ataagattcg attcataccc aaaaagccat ttcgatcata ggccgcaaga
     6121 tagacacagc aagcagctaa aacaccacct agaaaggcta caactagcaa tagaaacggc
     6181 agcgaatagt gcgaaaagaa acctaaataa agcatcacta aaaatcctgc tccggcatta
     6241 atgcccaata tactagaatc tgccaattca tttcttgtta ctccttgcaa gatataacca
     6301 gaaacgccaa gacaagctcc tgctaataaa ctgatcagta atcgaggaac tcgaaattct
     6361 ctaacaatca gcgccaaaga agatgattct ttccctatga acgtggcaaa aagctgatcc
     6421 aggtaaataa aaggcgtacc aatcattaaa ctacctatag acaggatgac aagtacaaaa
     6481 agcaagaaat agaacgtcca atatattttt ttcaccgttg ttccctcctt acttgtatca
     6541 gtaaaaacgg aacgccaatc aatgcaatga ctaatccaaa aggtgtttca aaagggggat
     6601 tgatcatcct cgctgtaaaa tccgccaaca taaccaatcc accgccaact aaaaaagaga
     6661 caggtagaac ataacgatag tccgttccaa ccattccgcg aacaactggc ggaaccaaca
     6721 gcccaataaa tgaaacaggt ccgactaacg aaacggcaat ggctgctaac actgcaacta
     6781 gacagaaaac aatcaaccgc gttcgtttag gatgacttcc aagagctaat gcatgtgttt
     6841 cactcaaatg aatcaaagat attgcttttc ctaatgaaaa agaaaccaaa acagcaagta
     6901 tatataaagg aaacaatact gctaattgtt tccatgtgat attggcagct ccgcctacaa
     6961 accaaaaagc aagatcctgc tgcagattaa agaattgggt aagtgcttgg ctgatagcag
     7021 taaaaaaaga actaatagct acgccagcta aaatcagctg taaaggattc atgcccattt
     7081 tcgaaaactg caaaaaacca aatacgagca aagttacagc aaaagaacct gccaatgaca
     7141 ttagaaacac agaaaaatca gaagctttag gccaaaaagc aaaactaagg gccattgcca
     7201 acgaagcacc cgcgttgatt cctagtaagc ctgaatctgc taaaggattt cttgtcactc
     7261 cttgcatcaa tgcaccgctc aatgcaaaac aactccctac aagaaatgct cccgccacac
     7321 gaggcaaacg aatatttcgt atgatttggt gactttgttt tgatctatca aaatcaaaca
     7381 acgcctgcca aaaatctgct ttttccacag tcgctgcgcc atttgaaaga gcaacaaaaa
     7441 aaataatcaa aagaacaaaa cagaccaaaa gcaggagtct atttttcttc atagtcctca
     7501 taattgatcc tcctgctctt ttggcaacaa atcataggtt agaaaaactg gtttatttgt
     7561 tcttggaagc gtgacaatcg ttgcatcaat ctcaaatatt tttttcatac actcttttgt
     7621 gatcacttct tcaggtgtac cttctgtgat caacttaccg tttttcattg ctaataaata
     7681 atctgaaaaa cgtgaagcat gattaatgtc atgaatcgac atcaagatcg ttgtttgttt
     7741 catttgattg atttcagtca ataactgtag gatctctaat tggtgtgccg gatctaaaaa
     7801 tgttgtcggt tcatccaaga tcaagatatc tgtttcctga gcaagcgcca tagcgatcca
     7861 gacacgctgg gtttgtccac cagacagcgt tgcgatatcc cgcatacgaa atccagctag
     7921 acctgtagca tccaacgccc actcaattgc ttcatgatct tgcgtgtcca tgccactgaa
     7981 cagtttttga taagggtatc gtccgtaaga tactacttct tctactgtca tacctgtagg
     8041 gtgttcacta gtttgtgcta ataatgcaat tttttttgca agttcttttg aactgtatgt
     8101 agagatatca gagttattga caatgatctt tcctgatttc acaggtagta tacgggtaaa
     8161 acttttcaat aaagtagatt ttccacttcc atttggccca atcaaactag tgattttccc
     8221 ttcaggaatc gtaacagaca tgttttcaat gatttttttg ttttcgtagc cggctgcaat
     8281 gtcagctgct cttaatctcg tcatttcacc catcctttcg ctgaaaatca ttctcaatta
     8341 tgacgctgtt tcaacatttc gtcaagcgct actttcatat ctatagccaa atgaagcggt
     8401 tgacagcgtt cgaaatatca ggaataataa ccaatgagaa agattctcaa ttatgttagg
     8461 aggaaagaaa tgaaattaaa aacaaaagta ttcacattat ctagcatatt gctgctggct
     8521 ggagtactga gcgcttgctc cccctcttca gataaagaaa cagctacaac tgccaattcg
     8581 acgacttcat ccaatagtac tcatacagtc acagatacac ttggacataa agtagagatt
     8641 ccaaacgcgc ccaaacggat catcggcagt tacttagaag actatctgat tgcactaggg
     8701 gaaaaaccta tcgcccagtg gactgttggc agtggttcca ttcaagatta tctgcaagat
     8761 gatttaaagg atgtcccaac gatttcttat gaccttcctt atgagaaagt gttaagcttt
     8821 gagcctgatt tgctgttgat ttcttcttcc gctactgtcg aaggcgggaa atacgaacaa
     8881 tatagcaaaa tcgcgccaac ttatgttgta aaaaacggaa cagatgtcac atgggaagaa
     8941 caactaaaag atgtcggcaa agctttggca aaagaaaaag aggcagaaaa aatcatttcc
     9001 gattatcaaa aatccatcaa gaaaactcga gaagaactag cagataaaat cgagaacaag
     9061 acagctgcag tgttgtgggt gaccaacaac tcagccttta tggtcagcaa aaatcggtca
     9121 agtggaagaa tcgtatacga cgatctggaa tttggtgttc ctgatctagt ggaagaggtc
     9181 tccaaaaaag ccacttctga ctggtctgct gtttcctctg aaaagctagc tgaattagat
     9241 gctgactaca tcatccttgt gaacagtgac aaagatgctg ccatgttcaa tgaaccaagc
     9301 tggaagaatc taaaagcagt gacagacaat catgtactgc aattcggacc tgaatctagt
     9361 tggctatata acggacctat tgccagtcag gaaatggtca atgatatcaa agaaaaacta
     9421 aattgatttt aactaaaaaa agcatcttca cgattagtcg tgagatgctt tttttcttat
     9481 taacaaacta gacgcataga ttttattctt cttttgaagc aaccctattt tttactcttt
     9541 cccatttttg aaaacgatgg gcatatagat tttcttgatc cgttatcccc tcatactcct
     9601 ctataagggc gaagtctgaa aaatcaatat tcccaatata cgtatcgcct tcaaacgtgc
     9661 tatcgatcaa tgttcgccag atccgaccag tgtaaggaag aagttcttga aaaatccgac
     9721 taccacccga aacatagata tcttctgaga tcgtttgact atacgacaaa agttcatcga
     9781 tcgaatgcat tacttccgca ttttctttcg tgcgaaaatt tggttgcgtt gtcaacacga
     9841 tgatatggcg ataaggaagt gaaagatccc ccattccttc ataggtcttt cttcccatca
     9901 ctaaaattcg atccattgta tgttcgcgaa agaaacgcat atcatttggc aatcgccatg
     9961 gtaacaaacc atttttgccg atcagaccat ttttatcttg tgcccacatg gaaataaaca
    10021 tattatccgc tccttatgat tttagatgtt ttgagataaa aaaagtctca attctttgac
    10081 cagcaaggaa ttgagaactt taaaaacatt attttgtttc acggtgcaaa gtaactttgc
    10141 gttcgcgtgg gcaatatttt tgtttttcca aacgatcagg attgttacgt ttgtttttgc
    10201 ttgttagata gttacgttct ttacaagaag tacactctaa agtgatgttt acgcgcatgt
    10261 ttttccctcc catttttaca agaatcctct aagatgactc tcagacttga ttatcttacc
    10321 atgatttttt ctagattgct agtgtttttc acgattttgt taagtaaaac tacttgacga
    10381 taaatacaac ggttttactt ctcaaacaaa atctttttct ttttgtataa atacccttac
    10441 acttgtttat atagattatg ttttttaata aagggggata ctaatatgcc atggaacatg
    10501 aaggactttc ctgcatcaat gaaaaactta gataaattga caagaaaaaa agcaatcgac
    10561 atcgcaaatg cattattaga tgaagggtac cctgataacc gtgcgatccc aattgccatt
    10621 gatcaagcaa aaaaatggta tgaagatgct agtgaatcag aaaaacgaac tttcgaaaaa
    10681 gaaaaaaatc caagcaagac ggatgaacac gatacgaatc ctcgtgctgg aaaactgctt
    10741 gattcagatg tgatcgtaga atatgaggaa gaacaatgga tcgtgaaatc taaaggggca
    10801 aaaaaggcaa gcaaccattt tgatacaaaa aaagaagcaa tcgaaaaagg aaagcaagta
    10861 gcacaaaaca aagaatcaac tttgttcatt tataaaaaag atgggacaaa agaaaaagaa
    10921 atcgattatt gaacatatgt aaaggggttg tgacgaaaat cgtgtcacaa cccccttatg
    10981 ttcgaaacaa tggtgttcct ctgccaactc cgaaccataa gtcataatat ctataaaacg
    11041 ggaagagctg ttttttgaaa ttgtttctta tcactcagtg ttaaacggta ggagaaaacc
    11101 tcttttttat tgatatttga ttttcatcaa acagtcacat agtcgattgc ttctgcttgc
    11161 ggaatcgttt catctacaaa gtagacttta taccatgtca agaaactgaa gattgtccag
    11221 atttttcttt gctcttcttt acgtccttcg tgatggtcat ctagcaactt catgattttt
    11281 tcttgatcaa aaaattcttt tgcaaaatct gcttcgaata atgctttgac ttgttcgtag
    11341 ccgtgttctt ctttcaacca agctttgatt ggcacaggga atcctaattt cacacgattc
    11401 gaccattctt ccggaagatg tttgttcgca gcttgtctga aaatatcttt tgtgttgttt
    11461 tgattcaata aaaacttact tggtatttgt tgagccaatt tcatgacttc gatatccaat
    11521 aaaggtactc ggacttccaa agaacttgcc atagaaagtt tatctgcctt taacaagata
    11581 tcttttggca tccattgatg aagatcgaca tattgcatct tattgacttc atctttgatt
    11641 ccttctgttt tctcgtaatg aacagtcatg atttcatcca cagatggtgc tgtttgatat
    11701 tttggtgtta acacttctga tgcttctgat tcttcgaaca ctcgagcatg accgataaaa
    11761 tattcttttg caggagcaag tgattcatat aaatggatcc gtccatggaa gtttttcatt
    11821 tttccgattt tacgtcctaa attatagcgt gttccttttg gtaatttttt caatccttga
    11881 gcaatcacac ggatgaattt tgaattcgta tggaatccat acgtttggta tcccgcaaac
    11941 agttcatccg ccccttctcc agattggact acccgcacac tttgtgaagc aagatttgct
    12001 aaaaagtaca aaggtacgca agaaggattt gaatcaggtt catctaagtg atactggatc
    12061 aaagggaatg ctttgaacga catgtctccg tcgatcactg ctgctgtatt atctaagtcc
    12121 aatagttcgg ttagttttct ggcttcaatc gcttcattat aggttttatc atcaaacccg
    12181 attgagaaag aatgatctgg tttcaataca gaggttacat aactggaatc aaccccgctg
    12241 gataagaaag aaccaacttc tacatcactg accgtatgtg cttcaataga agcttgaacg
    12301 gtttcgtcga ttttttcgat ccattcttgt cgggaatgcg tttcttttgt ttcaaaatct
    12361 gcatcccaat attgctgaat gtctattttt ccatctttat acgtataata atggccttca
    12421 ggcaaacgat agacatcttt gaagaatgtt tcgccgttta atggagaata ttggaaagtc
    12481 atataaggtt tcaacgcttc tttgttcaat tctttgttaa aatcaggatg aggcagaaaa
    12541 cttttgattt ctgaaccaaa cataaatgtt ccgttcattt ctgcataata atatggtttg
    12601 atcccaaagt ggtctcttgc accgaataac tcattctttt cattatccca aatagcaaaa
    12661 gcaaacattc cgcggatctt ttgcaataat tctgttcccc attcttcgta accatgcaaa
    12721 aggacttctg tatctgcatg tgtttgaaaa acgtggccag ctgcaatcaa ttcttctctc
    12781 aaaggctggt aattatagat ttcgccatta aaaataatga tctttgtccg atcttcattg
    12841 taaataggct gagtaccgcc ctctaagtca ataatactta atcttctgaa tcctaaagca
    12901 acatgtttat cgatatattc tcctgaacta ttaggtccac ggtgaacgat tcgatccatc
    12961 attgtattaa taattgtttt tttgttgtcc ttgtcgttaa caaaacctac aataccgcac
    13021 atttaacttt cacatcctaa tataaaatta ctgtttattt cattcatatg catcttatca
    13081 tagaataaat agcggacgaa tattttctct cttacaattt cgtcacgtga aaacaaaaga
    13141 tgaaagtttc tgtttttaca gaaaataaag tcccaatatc taatttacca tgttttcttt
    13201 tttgaggcta ccttattctc ttcgtgttaa aaaaatatta gtggatgcta tttccaaaaa
    13261 aaccagcaaa aaaagacacg acctaaagat cgtgtc
//
LOCUS       DANKHL010000071        13243 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000071 DANKHL010000000
VERSION     DANKHL010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.074"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(174..1472)
                     /gene="asnS"
                     /locus_tag="PW101_001444"
     CDS             complement(174..1472)
                     /gene="asnS"
                     /locus_tag="PW101_001444"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDL2513848.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(1512..2702)
                     /locus_tag="PW101_001445"
     CDS             complement(1512..2702)
                     /locus_tag="PW101_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2513849.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(2723..3250)
                     /locus_tag="PW101_001446"
     CDS             complement(2723..3250)
                     /locus_tag="PW101_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HDL2513850.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(3297..6086)
                     /locus_tag="PW101_001447"
     CDS             complement(3297..6086)
                     /locus_tag="PW101_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2513851.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(6233..6433)
                     /locus_tag="PW101_001448"
     CDS             complement(6233..6433)
                     /locus_tag="PW101_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2513852.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(6886..7206)
                     /locus_tag="PW101_001449"
     CDS             complement(6886..7206)
                     /locus_tag="PW101_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513853.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(7709..8827)
                     /locus_tag="PW101_001450"
     CDS             complement(7709..8827)
                     /locus_tag="PW101_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513854.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            9298..10605
                     /locus_tag="PW101_001451"
     CDS             9298..10605
                     /locus_tag="PW101_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL2513855.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            10726..11925
                     /locus_tag="PW101_001452"
     CDS             10726..11925
                     /locus_tag="PW101_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL2513856.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(11952..13220)
                     /locus_tag="PW101_001453"
     CDS             complement(11952..13220)
                     /locus_tag="PW101_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HDL2513857.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVRKGILFLPIVKRMKEKNKV"
BASE COUNT         3808 a         2816 c         2391 g         4228 t
ORIGIN      
        1 cacaaaattt ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg
       61 gtattcaaaa gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg
      121 tttttgcttg gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga
      181 taaattcggt tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca
      241 atccaagtga ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat
      301 ttacgtagat caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt
      361 tctagcaaga aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct
      421 tctggtgcga tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg
      481 tagaatggct tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc
      541 gcaatgaacg tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca
      601 tttttcttca gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta
      661 taagttttta agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct
      721 aaaacatttt gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga
      781 tgagtgaaag ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt
      841 gatttttcag cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca
      901 gcttccatgt ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt
      961 tcgaatagat ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcgattttg
     1021 acaaaatgat gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc
     1081 ataatcgcat gttgtcttga agaacgcagc cataaatgac gatgatccat caagaaatct
     1141 gttccgtgtt cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt
     1201 gtaattccga tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact
     1261 aacacggatg tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg
     1321 ctttttacaa ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt
     1381 tttccgcttg atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg
     1441 tttttcgaat cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa
     1501 tttcttcagg attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct
     1561 tcttctaacg tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag
     1621 ccttcgccag tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg
     1681 acattttcat acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct
     1741 ccttgcggct tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc
     1801 aggcgttctt caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct
     1861 tcaacagctg catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg
     1921 atcattccat tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc
     1981 gcatatgttt tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc
     2041 gttgcaattg gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca
     2101 actatcaaaa tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta
     2161 taaatcattc cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa
     2221 cgtgcttgtt ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca
     2281 aagacaggaa gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca
     2341 ggaatgatga cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat
     2401 ttcgctccgt ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc
     2461 agataatcaa cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta
     2521 gctttcccat tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa
     2581 tccggttctc ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc
     2641 tttgccgcag ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc
     2701 atcccattca ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt
     2761 caaagccaat caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca
     2821 caggctgatc atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg
     2881 cagcaattgt ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta
     2941 ctttttcacc agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg
     3001 tcagtgaaaa gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat
     3061 ttgcatattt ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg
     3121 aacgcaagta aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa
     3181 gtaacacgcg attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct
     3241 ttatcttcat tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa
     3301 cttccttctt catttttctt taagaattca tggatatcct gatagatttc ctccatagct
     3361 agctctttga tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca
     3421 gtgactagtc ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag
     3481 cgacctaatg cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga
     3541 ttgatcccct ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta
     3601 tcgaagggta aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct
     3661 tcccaaaagc tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa
     3721 agcttctccc ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata
     3781 gacagatgca ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt
     3841 attggccgat cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat
     3901 gcttgattgc tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt
     3961 ttctcataat cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc
     4021 ttagagagat agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga
     4081 tcatatcgag gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga
     4141 aaaatcatct gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag
     4201 tcttccagcc attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat
     4261 aaacttgcta aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc
     4321 agacgcattt gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg
     4381 gctgtcccag attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct
     4441 ggatactgtc cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa
     4501 atctgtgctt cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct
     4561 tcatgttctt tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat
     4621 tgctggattt gtttatggaa atagttaata cttaatcgtt tgtcatttac tttttcagcg
     4681 atttccggca aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt
     4741 tcttttcgct gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc
     4801 ctgattttct tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct
     4861 aaaaatccaa tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa
     4921 tgtaattcat ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga
     4981 tacaaagcat attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa
     5041 tcaatatagt ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga
     5101 ttcagcaatg gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg
     5161 atgacagcgg gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt
     5221 ttacccattc ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg
     5281 tcgtagaccg cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac
     5341 attttttctt ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg
     5401 aatagttcca cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca
     5461 agtggctctg gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc
     5521 atacctgtgt gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc
     5581 atccgttctt cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga
     5641 tgaggattct catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt
     5701 tccgtcggca agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta
     5761 ggaagtccgc atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc
     5821 gcgacaaata tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa
     5881 ggcgcttttt ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc
     5941 ttccgccctg gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg
     6001 acgcagccaa attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca
     6061 acgactgcat aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt
     6121 ttgaaattat actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca
     6181 aaaacgccca gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt
     6241 tagtaacgtt tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt
     6301 ggccttcttc taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat
     6361 cgtttccgtt ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg
     6421 tacctgtttc cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga
     6481 atggtgtata tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa
     6541 ctactcctat agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat
     6601 taaaaaaagt tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa
     6661 cgacatattg ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt
     6721 attctttctg actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag
     6781 taggtctgat gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc
     6841 aaaccaatac gaccaacgag attagttttt cttttttgct catatctata cctcataaat
     6901 aacaggatga tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga
     6961 atgtaagaaa tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa
     7021 tcttggctgc aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg
     7081 tacataaaca tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc
     7141 aaaaactggc tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt
     7201 tttcataaag acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc
     7261 ccgttattca cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt
     7321 cgtgaccacg tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata
     7381 agccgaaatt tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa
     7441 gctgaccact tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt
     7501 aataagccca ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg
     7561 atgcagaaca gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta
     7621 ttagctcaaa aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca
     7681 ggtcaaaaca ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt
     7741 catctggcgc atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat
     7801 ctgctaatgc attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac
     7861 catcagctaa gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg
     7921 gaacgatatc aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg
     7981 attcttgagc tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg
     8041 atagctgcgc aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa
     8101 tttttttgaa catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg
     8161 gattttcgaa agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga
     8221 tctctacttc ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc
     8281 ctcctccatt agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa
     8341 cgacatcaac cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt
     8401 catacaattt catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc
     8461 catttttgat aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg
     8521 ggatactcaa acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag
     8581 ttgcactacg ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg
     8641 accagtcatg ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt
     8701 tgaatttgat ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg
     8761 tgacgaatgg tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt
     8821 ttgccataaa cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca
     8881 caacactatt tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt
     8941 taaatttagc tattttttta agttatcttc ataatttcct caattattta gccccagtct
     9001 tcaactattc ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta
     9061 tgcatttgat tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt
     9121 cccgcttatt agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct
     9181 aaccttccat aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc
     9241 cctctctgat aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg
     9301 tccataaaaa aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct
     9361 tttctaccag ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc
     9421 tttgctgtag atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg
     9481 gggatcgctt caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa
     9541 gggaaactat catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc
     9601 accccagatt tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta
     9661 ttcgactctg caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc
     9721 tctggcagcg aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc
     9781 aaagatgcaa cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga
     9841 ccagaaggta ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt
     9901 gcccgtcatt tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa
     9961 atgtttggcg aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca
    10021 ggattcgtga attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca
    10081 ggagcctgtt tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg
    10141 aatgcagacg gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg
    10201 gattattgct acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct
    10261 agtctaaaaa ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa
    10321 agtcctgtaa aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca
    10381 actcttgaca aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca
    10441 aaaatcggta ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac
    10501 aacgcgccat ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt
    10561 attggctggc gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa
    10621 aaagcatctt tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt
    10681 ttcatctatt ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga
    10741 gatctgattt ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa
    10801 cagaaaaagg atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg
    10861 acttattttg tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg
    10921 tttttcagta tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt
    10981 ttattcaata ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca
    11041 caaaatattt ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt
    11101 ctattcggca tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt
    11161 agagagtcgc attcattagc taatctttta acgttgatac tagccatttt tcttacattg
    11221 tttcttgttt ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt
    11281 ttctgcatcc cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc
    11341 tcattcttgt atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc
    11401 gccgggttga tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg
    11461 attgcttttt attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg
    11521 ggtggacagg gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg
    11581 gaacaaggaa ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag
    11641 aatatgcgct attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc
    11701 ttcagttcca acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg
    11761 aaggcaaatg gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga
    11821 gaatacatcg ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt
    11881 ttcgcaggtt ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga
    11941 caaaacatat gtcacacctt gtttttttct ttcattctct taactattgg caaaaagagt
    12001 atcccttttc tgaccgctac ttgctgagca gaataaatac cgctgttgcc tgaacacata
    12061 aagctgatca agcagatcat aaagaagtat actgccgctt gtgaaccgaa caattcgatc
    12121 cccatcacaa agcaggcaat cggtgtattt gtcgcaccag aaaatactcc gataaagccc
    12181 aatcctgcaa gaaaaggaac agaaacatgg agcaaaggcg ctagtgctgc accaagcgtt
    12241 gcaccaattt caaacaatgg tgtcacttct cccccttgat atcccgcgcc taaagacaaa
    12301 actgtgaaga ataacttccc aataaaatca tagacatgtg cctgtccatt aaatgattcc
    12361 tgcagtaatg gaagactcaa tccgagataa cgttgtgttt gcaaaatcaa tgctgcggct
    12421 acaactacag ccccacctat cgtatttcgc caaacaggat gaggaatcca ttgtgcatat
    12481 cgcgctttta aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc
    12541 ccagcaacaa aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg
    12601 taatgtagat gagaaacacc atagctttct gtcataaaat ttgcaaacag tgcagcaaag
    12661 aagctaggga agatcgcttc tgttcggact ttcccgattg ccaacacttc taatccaaat
    12721 aatgttccag ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata
    12781 atcaagattt cccgttctga tttatccaat cggaacaaac gtgctgtatt atctgctaac
    12841 gctcccccca tttggactgc tgttccttca cggccaacag atcctccaaa taaatgggta
    12901 gttatggttc caaacagtgt aagtggaata agtcgcaacg ggattttttc ttctccacca
    12961 tttccttgat cgatcactaa gttgtttcca cgactagaaa gaccgccgtg atacgcatag
    13021 agataagcaa aaagcgcacc actaactggt agtagataaa ggagccaagg atgtgatagt
    13081 cggatatctg tcacaactgt caggctattt aagaaaaaag cagataagct ccccatgaat
    13141 ataccgagaa tcgctgtgat caccagccac ttcagcatgt acactaaaat gcgtactgga
    13201 ctcggtaatt gtttttccat aaaaaattcc tcctcaataa aaa
//
LOCUS       DANKHL010000072        13155 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000072 DANKHL010000000
VERSION     DANKHL010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.075"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(118..432)
                     /locus_tag="PW101_001454"
     CDS             complement(118..432)
                     /locus_tag="PW101_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDL2513858.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(446..706)
                     /locus_tag="PW101_001455"
     CDS             complement(446..706)
                     /locus_tag="PW101_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDL2513859.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            876..1190
                     /locus_tag="PW101_001456"
     CDS             876..1190
                     /locus_tag="PW101_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2513860.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            1192..2847
                     /locus_tag="PW101_001457"
     CDS             1192..2847
                     /locus_tag="PW101_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2513861.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            2930..5065
                     /locus_tag="PW101_001458"
     CDS             2930..5065
                     /locus_tag="PW101_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2513862.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            5240..5749
                     /locus_tag="PW101_001459"
     CDS             5240..5749
                     /locus_tag="PW101_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513863.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            5746..7050
                     /locus_tag="PW101_001460"
     CDS             5746..7050
                     /locus_tag="PW101_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2513864.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            7146..8015
                     /locus_tag="PW101_001461"
     CDS             7146..8015
                     /locus_tag="PW101_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2513865.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(8127..8447)
                     /locus_tag="PW101_001462"
     CDS             complement(8127..8447)
                     /locus_tag="PW101_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDL2513866.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            8550..8996
                     /gene="dhaM"
                     /locus_tag="PW101_001463"
     CDS             8550..8996
                     /gene="dhaM"
                     /locus_tag="PW101_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDL2513867.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            8971..9981
                     /gene="dhaK"
                     /locus_tag="PW101_001464"
     CDS             8971..9981
                     /gene="dhaK"
                     /locus_tag="PW101_001464"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDL2513868.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            10009..10614
                     /gene="dhaL"
                     /locus_tag="PW101_001465"
     CDS             10009..10614
                     /gene="dhaL"
                     /locus_tag="PW101_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDL2513869.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            10761..11426
                     /locus_tag="PW101_001466"
     CDS             10761..11426
                     /locus_tag="PW101_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDL2513870.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            11931..>13155
                     /gene="ltrA"
                     /locus_tag="PW101_001467"
     CDS             11931..>13155
                     /gene="ltrA"
                     /locus_tag="PW101_001467"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL2513871.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRL"
BASE COUNT         4470 a         2347 c         2830 g         3508 t
ORIGIN      
        1 acttgatgca ggacagcgtt ttcacaacct ttgattcacg gttttccaga agtagcttct
       61 tcgaaaaata agccgaaatt tcttgaagct gatcacttct gtcgcaacct ctactgacta
      121 aggcttgata taagcataac cttgggattg cagttcaact aaatcaagga cacctgctgg
      181 tacaacgatt acttgttcag gtaaagaact ttctgaaata tgattagcac gcatagcatt
      241 ggcgcaagcg tgaaaaacga ctccttgcaa atctaagaat tctgcattgg cagagtctag
      301 ataaccggta atcgcgatgc catttacaga tacgacaatc gtcgtttctg gagaagcttt
      361 tatcaggttt tttacgtttt tccctgtttc tgaccatttt tctaattcat caatatggaa
      421 aacgactttc attgaaatca ttcctttact ttttaataat catcttgatc gcttgttcta
      481 gtttagccgc ttgttcttct ggattactat caggtttctc aaagcaattt ttcagattat
      541 ctgcaacgat catgcccatt acgcgatcaa tactagaacg aacagcgctt aattgagtga
      601 tgacatccat acattctttt tcgtcttcga tcattttttg gataccacga atttgtccct
      661 ctgtacgttt caaacggttc aggatatttt tattatcaat ctccataaca cattctcctc
      721 tatttttata attagataat accccttatc ggtattttat gcaaatataa aaagtagtga
      781 aaaaaatgaa gagtcattct ggttgacata ttaataaagc tcctgtttaa tataccccat
      841 gaggtattta taaaaatgta aatgaggtgc agaaaatgaa tttgctattt caatcaataa
      901 aatcgataac agtcccagaa ttaaaggaaa agcttttgga aaatccagcg ttacttgatg
      961 ttagaacacc tgcagaatac cggggcgggc atataaaagg agctaaaaat gttccattac
     1021 aaagtataaa cagatatgat ggagacaaag aaaagaccgt ctatgtgatt tgtcaatcag
     1081 ggatgaggag taaacaagca gctaaagaat taaagaaatc tggttatgat gtagtgaatg
     1141 tacgtggtgg catgaatcaa tggtttgatc gaacagtagg agggaaataa gatgaaaatc
     1201 gttattgtcg gaggtgtagc aggtggaatg tctgctgcta cacggcttcg ccgattaatg
     1261 gaagatgcag aaatcgttgt ttttgaaaaa ggaccgtatg tttcttttgc aaattgcggc
     1321 ttgccttatt atctttcggg agaaatcagt gaacgggaaa atcttcttgt ccaaacgcca
     1381 gaatcattat ccgctcgttt ttgtttagat gtgcgtccaa atcatgaagt gacagccatc
     1441 tttcccgaaa acaaaacggt agaagtcgta catgagggtc aaaaacacat tgaacagtac
     1501 gatgcattgg ttttatctcc tggtgcaaaa ccagttgttc catcgattcc agggataaca
     1561 gaagccgaca atgttttttc tattagaaat gtaccagata tcgataaagt gatgcatgca
     1621 ttagaaaaac agccaaagcg tgccgtgatc gttggtgcag gattcatcgg attggaaatg
     1681 gcagaaaacc taaaaagaag aggtttagaa gtcatggtga tcgaacaagc accacatatt
     1741 cttccaacac tggatgaaga aatggcagct tttatagaaa aagaattgtc tcatcaagga
     1801 gtagaagtga ttacttctca tgctgtcgct ggatttgaag accacgggaa acgattgcga
     1861 ctggatgatg ggcgtaccat ccctgctgat ttagttattt tatccattgg tgttcgtcct
     1921 gataaccagc tagcagtgac cgctggaatc gaattaggca tacgcggggg tatcctagta
     1981 gacgaacgtt atcaaacgaa tattcctgat atttatgcgg tgggggatgc tatcgttgta
     2041 aaacagcaaa tcactggaaa agatgcactt atttctcttg cttcaccagc caatcgtcaa
     2101 ggtagacaag ttgcggacac gatttccgga atttctcgaa gaaatcaagg cggtattgga
     2161 acagcaatta tacgaacgtt tggaatgact gccgcatcca ccggtttaag tgaaagaaca
     2221 gccaaagaaa acgaactgtc ttttgaagtc attcatgtat caggaaaaga tcatgcaagc
     2281 tattatccag aagcaacaga tattttactg aagttgatct tccatccaga gactggcgag
     2341 atttatggtg cacaaggtgt tggggcaaaa ggtgtggata aacggatcga tattttagca
     2401 acagcaatca aagggcattt gacgatcttc gatttaccgg aattagaatt gacgtatgca
     2461 ccgccatttg gctcagccaa agatccagta aacatgctag gatatgcagc aatgaacatt
     2521 gcagaagggc ttagtgaaac cgtacaatgg catgaattgc cgacagaact agcaaaagga
     2581 aaaattttat tagatgtgcg aacagcagaa gaattggaaa agggcaaatt caaggaagcc
     2641 aaacatatcc ctttgaatga acttcgggac cgattaaatg aattagacag ccaacaagaa
     2701 tatatcgtca gctgtcatag cggattacgt agctatctag cggaaagaat cttgaagcag
     2761 tctggctacc acgtaaaaaa ccttgatggt gcattttctt tatatcaaac tgtccgacct
     2821 gaagaactga tatatcctaa caaatgatac tcgttaaaaa gcttgcattc gactcttaaa
     2881 agaggtaaac taatcggttc caccacttta aaaaaagagg agaatgtgta tggtcaacac
     2941 acaaaaacag ttagaagaag catatctgaa gaacgtgtat caaaagctaa ttgataaaaa
     3001 aaacgaactg cgggaatcga tggatacggc aaaaatagaa ggaaaccagt cgttgaaaga
     3061 aatggctggc gagcttcgat tgaattttga cagttactta gataacctgg atactttttc
     3121 gatgattgaa atgaaaaata gagaaatcga tcagatgaat atcaaacaga aaaacgcaga
     3181 aaatcagcta gcaaagatcg aacgtttatt gaaaagtccc tactttggaa aagtcgttgt
     3241 agactttctg gataatgagc caaaagaatc cttttatatc ggtacagcaa atttcacaga
     3301 tgatgcagat gaaaatcttg tttatgattg gcgttcacca attgccgaac ttttttacaa
     3361 taacatgatt ggtccatctt cttatacggt tcgtcaaaat aaaatcgata cggttattaa
     3421 agaaagacga caatttcttt tggaaagaga tcgtttggtc aagttttttg atacgtctgt
     3481 agctattcaa gatgacgtat tattagaagt cttaggccaa gatgagacca atgaaatgaa
     3541 ggctatcact gctacgatcc aatcggaaca aaacacaatc attcgggatg tgaaaagtca
     3601 aaatatcttg gtaaatggcg tagcaggaag tggtaaaacc tctgctatca tgcagcgtat
     3661 tgcgtatctg ctctatttat atcgagatac gatgacatcc gatgacttgc tgattctatc
     3721 acctaatcat cggtttatcg actatatttc gaatgtccta ccatcattag gtgaaagaaa
     3781 tccactgaat ctaaccatca tacagctggt ttcacagctt tcagcagagg aaatagaagg
     3841 tgaagaggct tatttcaaac gtatcacggg agaaaatgta tcggagcaga cagaacgttt
     3901 gcgtagcaaa aagtttatcg ataatctaaa acaatcagat cctttgtttt tagatcatcc
     3961 gaattttatc cgtggactta ctaaaaatgg aaaaacagtc ctttctaaaa aaacgatcga
     4021 aaaaatatat gagaaagttc ctgctcatcc taaattgatc gataggcttc aagcaacgaa
     4081 aaaggcactg atgagcgaat ggaagaatca tcttttgaaa caagcaaaaa gtcctgctgt
     4141 gcagaatcag gtactctcgt tgacagaaga taggcagttg gaattattcg gtaaattgat
     4201 ttctgatgat tcggaacaaa gtattgtagc ttatgcaagg aaacttttac aaaaaaaata
     4261 tcgaaaaata accagacaaa tcgaagaaat ggcatgggtg gatgagcatc agttgtttga
     4321 aagaatttat gaaaaacgat atggctcagc ttatgcttgg caacccacac gtactgttga
     4381 tgaagcagtc attatattag ctattcgtca tttacttgtt gaaaaggtaa atgtaccagc
     4441 atttcgctat ctattgattg atgaagtaca agattatacc ctggcacaat taggtctatt
     4501 gatcgaatta tttccaaaaa ctcactttac tttagtaggt gacgagaatc aggcaatctt
     4561 taattcttcc actacttttg ctgatattat gcgttgtttc gacgactacc atctgccgat
     4621 ccatcggtat gatcttagaa atagttaccg ttctagcgga gcaatcacag agcttttcaa
     4681 aacctatgct gtggatcaag agaagatcga catcgtatcg atccgaccgc aaggaaaaga
     4741 acccgaatac cgctcttttc gatcctctga agaattgttg aaaatactag ctgagatttt
     4801 ggtttctcta aaaggagaaa aagcggcagt tatcacccaa acagaagagg aaacccaaac
     4861 gctacaagaa tcgattaaaa aaaattcata tggattagaa aactgccaaa tcattccttt
     4921 gagtctagca aaaggactgg aatttgatca tgtaatctta tatccatttg aaaacgatgg
     4981 agatgagcaa agaagacgca gacaaatgta cacggcgata tctagaggaa tgaaaagcat
     5041 tgtcgtattg gaaagagcaa cataattaag agataaaaaa taaaaagaac aactgataaa
     5101 aagaacaaaa tatttttcat ttgctgatga gaaatgtttt gttctttttc ttaggaaaaa
     5161 tttaggaaca ctttatgtga aaagaaatga aatgtatggt aaactcctac gtcgtaacaa
     5221 aaaaacgtga ggtgaggata tgaaaaaagt aacaagagaa gaagttgcat caattttggt
     5281 gaaagataag tatttttcaa aaggtaatta ctggttaaag atttggcaaa cgttagtggc
     5341 tcttttagga tggatgatcg ttgttcttcc ttttatatgg gtctttttcc cattaacgag
     5401 accagaaaga gccaaggatt tttccttgta cgctttttta tcagaaaaga aaatgctgta
     5461 tttcttaatc ggcttttttg tattgatttt tttcagtttc ctgattacct catttgtatt
     5521 gactaggtgg aacaatcgaa atctatacaa aaaagtaaat aaatcttttg aatatgaaga
     5581 tgaaaaattg aaaaaaagac aagaattact tgaagaagtg tatacggaac gttttggaac
     5641 aaaagaagag agagaaacgg tccgttttta ttctgtctcc gaagagaaaa atctagatac
     5701 gacattcatt gcagatttgt ataaagaaaa tggggtggaa ctgaaatgat ctcaatcacc
     5761 catacaggta atttattctt agatacttgt ttgtctatta tgtatttttt tctcgtttct
     5821 tatccgattt taggcggatt tgtatggttt attggtgtct ggtgttatgt gtttttatac
     5881 aaacataagc aaaaagaatg ggtagatgtt cccttgagtg tcgaaccgtt catcacgatc
     5941 atggtgccag cccataatga agaaattgtg atcgaatata caatcgagta tttgatgacg
     6001 aaattgaatt atcacaacta tgaggtatta gtgacagacg atggttcaac ggaccaaaca
     6061 ccagaaatat tagctagact gatgaaaaaa tacgctaatc ttcgtgtggt tcggatcgaa
     6121 aaaaataaag gaaaagccca cgcgtttaat atcggacttg cctttgcaaa agggaaattg
     6181 attttaagca atgatgcaga cacggtaccg gagccagatg cactgattag gtatgtgaat
     6241 tatttcattc gccctggtgc aagacatatt gctgcggtca ctgcaaatat ggatgtccaa
     6301 aatcgtacca aattgattgc aaaatctcaa acggttgaat tctcaagtat cgtgggcatc
     6361 attaaacgaa cacagtcagc tgttttcggt ggtctttatg cgtacagtgg agcaaatacg
     6421 atgtatcgaa aagaagcctt gatcgatgtg ggcggattca gacaagatcg agcgacagaa
     6481 gacatcagta ttgcttggga tcatcagtta aatgactggt tatctgtttt tgctcctgag
     6541 attatctttt tcatggaagt acctgtgacg ttgaagatgc tatatcgaca cagaaaacgt
     6601 tgggcaaaag gtggaacaga agtctggctg acgaatttca aaaaagtgat gctccatcct
     6661 tttaaacata ttggaagaac gatcatcttc attgatcaaa cattgagtat tgtctggtca
     6721 atattctttt gtatttccgt agttcttttt gctggattaa tcgggcatta tgtatatcaa
     6781 ggaaactacg aacagattta tattactttt acattcagtt ttgtgttcat ctgctttgaa
     6841 atggtcgcag gatttttcca gttgctagct tcgctgattg tagatgatcg acgaagaaaa
     6901 ttgaaatacc ttcttttcgc accactatat atgcttttgt tctggatagt gaacgcgata
     6961 acgatcgtga cgactttcat tccagcagtc aaaacgattt taggttatgg aagcgggaca
     7021 tggaaaagcc cggaacgtac aaaaaaataa aagatgcacc gttttttaga caaacagcct
     7081 gttgtgttta tggttcttcc aaacattttt gcttataata agaaattagt taaaaagtag
     7141 taaggatggg aaaagtgatg aaaaaaagaa tgaagccgtt aatctggcca attgctgttt
     7201 ccagtatttt cttattggga cagacagctt ttgcggaaac ttctgaacct gctcaaacag
     7261 aaccttcaac aaccataagc tcttctggtg ctgaaaacgg tgcctcggaa acagaaatga
     7321 cttccaatgt accgacagat aattcaggag aaacgatttc atcttcagaa gatacatcaa
     7381 taaatagttc tacctcttct gaccaaatcg atcatttgca agacttggaa aataaaagaa
     7441 aagaactcga agaagcatta gaagctgcaa aaaacaaatt gaaagaagac caagcagcaa
     7501 tcgatcaaga tttagctgat cttaaaaata aagtgggaaa tgaccgtgca caacttgaga
     7561 aaaagcttgc cgaagcgaaa gcaaaagcgg aagctgtcca aacagatctt gacgggaaga
     7621 tccaacaagc aaaagaattc aatcagcaat taaatcaggt tgctcaaaca gcttctggaa
     7681 agctaagtca aactcaagcg gatatcacag cggccagtca agcaatccac ggaaagatca
     7741 gcgaagcaca aacagttgct aatgattttg aagcaaaaaa agctgactta gcagctagtt
     7801 tccaacgaat aaaagatatt ttgcattcgg caggagaaaa atcagtgacc tctggaagca
     7861 cgacagaaac agttcagcaa acgacagagc catccaaatc aacaagtcag actttgccaa
     7921 aaacaaatga aaaaacagct tctgtcagtt attccttagc aggggcgggt gtgttagtcg
     7981 cagcagctta cggaatcatc cgcaaaaaga aataaaaaag agaggatggg aaggtagagt
     8041 acctcgaagt tagactgaaa aatctaattc ttgagtgcca ctacatgtcc catctctctt
     8101 tagttattaa aacagatgtc ccctacttat ctcgtggaga cagctgttcc actacatgtg
     8161 accatcagca tgccattgtc gctgcctaat acttcatagt ccatttttac gccaatgatc
     8221 gcatcagctc ctaatgcttt tgcacgttcg gccatttcgg caagtgcttc ttctcttgca
     8281 cccattagtt cgttttcata gctttttgag cggccgccaa agatatttcg caaccccgcc
     8341 tccaagtctt taaatgcatg gatcccagta atgacctcac caaaaacgat ccctttataa
     8401 gctactacgg cttttccctc tacactgttc gttgttgttg taatcattcg atcactcctc
     8461 tcttttaatt aaagtataac aaacttcaag aagcactaca catattttcc caaaattaga
     8521 aatttattat atacttgaat tgttcattaa tgaaagcgaa accaatcgtt agcaactatg
     8581 taaaagaaag gaagattctt gtgaaaaaaa gtattttgct tatttctcat agtcaaaaag
     8641 taacagatgg tattaaagaa atgattgagc agatgcaaaa atccgaagag gtgcaaattt
     8701 tttctctcgg cggaataaat ggcgagatcg gatcagaccc gatgaaaatc gtagaagcag
     8761 tcaatgaatc aagcgatgca gctagttatt ttgtttttgc ggatatcggt agcgcggtat
     8821 tgaactcgga actggcaaaa gacatgttgg aagaagagca gcagcaacgt tatcatctgg
     8881 tggatgcgcc tttagtggaa ggagcttttg cagcagccat cactgccggt ataacagatg
     8941 atactcagca aatcttaaca gaagcacaaa atgccggaaa gaaaggatgg gaataaattg
     9001 aaaaaaatca tgaatgatcc aagcaatatt gtcgaagaaa tgctggaagg attagtaaaa
     9061 agctatccag aacttgttca tcgtgtagaa tcttcccgtg ttgtagccaa gaatcagaaa
     9121 gcggaacaag ttggtttggt ttctggtgga ggaagcggac atgaaccatc ccatgccggg
     9181 tttgtagggg aagggatgtt aagtgcagcg gtgcttggtg atgtgtttac ttctccaact
     9241 ccagatcaga ttcagacggc tattaaggaa gccgatagtg gaaaaggagt tttgctgatc
     9301 gttaagaatt atacagggga tgcattgaac ttcgatatgg caaaggaatt agctgctatg
     9361 gacgatattg aagtagaaag tgtgatcgta gatgatgata tcgccgttga aaatagtacg
     9421 tatactgctg gaaaacgcgg agttgcaggt actgtccttg tacacaaaat cgtgggcgat
     9481 gcagcaagaa acggtgcttc cctagctgag ttgaaagaac tgggagaaaa ggtcgtacag
     9541 gcaactaaga cgatcggtct agcgcttcga gcagcaactg taccagaagt agggaaacca
     9601 ggattcgaac taggagaaga tgagatcgag tatggtgttg gtatccacgg tgagccaggc
     9661 tatcgtcgag aaaagatgca gccttctaaa gtactggcaa aggaacttgt tactaagatt
     9721 ttagatgatt actcgaattt accaaaagaa gcaggtgtac tagtcaacgg aatgggcggg
     9781 acgcctttga tggaacagtt cgtgttcatg aatgatgttt tagctctgtt ggaagaaaga
     9841 ggcgtcaatg tagttttccg aaaagtcggt aattttatga cctcactaga tatgcaaggt
     9901 ctttctctaa cactgatcga cttgacggag acacaatgga aagattcttt agaaagcaat
     9961 gtacagacga tttcatggta aaaaatctca agataaaagg gtgataatat gaaactaacc
    10021 gttcaagaaa tccaagaatg gctcacgcaa tttgcagagg caatcaatca gaacaaacaa
    10081 tatctcagtg atctagatac cccgattggc gatggagacc atggaaataa catgggaaga
    10141 ggggtttcag cttatgaaga agcttttcaa gccgatcatc ctgaaacaat cagcgataca
    10201 ttcaaagtat tctctatggc gatgatatcg aaagtcggtg gggcttctgg tccgttatac
    10261 ggatctgcat tcatgaacat gatgaaagcg acaaaagagg tcgattctat tgattctcaa
    10321 gaaaaattag gcgaagtgat cgagcaggga acagcgggta ttcaaagccg aggaaaggca
    10381 gaagctgacg ataaaacgat gttagatgtt tggctgccag tcacagaggc attgaaggca
    10441 gggaatttaa caaaagaaat cattgaccag gcaaaagaac acaccaaagt cttggtagct
    10501 aaaaaaggac gagcttccta tctaggagag cgtgcaatcg gacatatcga cccaggagct
    10561 gcttctagtg ccattttatt ttcaacgctt ttagatgtga tcaatcaacc ttaagttcaa
    10621 taaaactgcg gtgatgctca tcgcagtttt ttgtttggct gttcttatga cttgatgcag
    10681 aacagctttt tcacaacctc tatttgtcca tatcgcaaaa ttcaggtaga atacaaaaga
    10741 taacggaaag aggcgaaaca atggaacttg gaaaatcaga ttatcagttg tttgatcgac
    10801 caatctacgc attcaaacag ctcaaagaga gccatccaac agataagatt gaacaaatca
    10861 agaaagaata taaagaacac tggcaaaaat ggaaagagat tcagctccag acagctgcac
    10921 ttttgcctga tatgtacggc atgagcaaac ctaaaatcga aagctggacg aatggctgga
    10981 atttacgcag tcatttttgg agcgcgtacc gaagcgaatc tcgtcaagac gagaatgcct
    11041 gtctagcagt tttgttgaat caaaaacaat accaaatcta tttgatgtac caacattata
    11101 aaagcgaaga acgatatggt agcattgcag cgtataacca gttgcttgcg atactgaaag
    11161 agtggagcaa aacagtcgat atcaaggatt attacatatg gccgcaacca gaacatgaac
    11221 ttgacgacca cttagctttg tctgactatt tgtccgatac aaagaaacaa gaagagttga
    11281 aaaaagcgat gagagaccga acctttcaaa tgggtaaact attcttctat ccgcaagaaa
    11341 tcgaacacgt tgagcaaata accgctgaaa ccttacaaga actagcacca ctatatcaaa
    11401 agctaggtgc ggaaaaattt caatagacaa cacgaaataa gcaatgcgaa aaacaaacct
    11461 tactctcggc ttgcgagtga aggtttgttt ttgatgtgcg ccctgcatgg gtgaaaactt
    11521 ggtggtgaaa gtccactaca ggcttggcag taggaactgt tagcgaaaga caaggcggca
    11581 atcgtgaggt tgtggctgaa ggaagtcgga cgcaaactcc tgaactgacg aacagaaact
    11641 agatacaagg ctgaattagg tcggataagt cttcatcaca agacaaagtc caatactgcc
    11701 cgaaacctat acagtaaatc tagcagttac atgggaggaa agttttcact cttaccgggg
    11761 gagatctcat gagggtgttg tgggcgttga atgaaaacaa accagcacaa cgatcatggc
    11821 gacatgatgg tgaatcatga gaagtcagcc gaggtcatag tagtaacgac actgaccgtt
    11881 acgaaggact gaacaataag aatcttgaat aatttaggag gtgtgagaag atgcgacgac
    11941 cgcagaaaac aagaaaactt ggctatcatc aaagagctag tgtggaacac gaagggtatg
    12001 atgaagtgcg cagcacttct tgcggtgaac agaggaaaca agatggtgta gaaaatctgt
    12061 ttgggagaat tctagatcgg aataatctca atcaagcgta tcttcaagta gtcagaaata
    12121 aaggggcggc tggtgcagat gggatgaccg tcgatcaact gcttccttac ttgaaagagc
    12181 accgcgagga attactatcc aatttacgcc aagggaacta taaacctcag cctgttttgc
    12241 gagtagaaat accgaaacca aacggcggtg taagaaaact aggaattccc acggttattg
    12301 atcgtatgct tcaacaggca atcaatcaag tgttacaacc gctatttgaa ccagaattct
    12361 cagataatag ttatgggttt cgcccaaatc gcagcgcaca tgacgcagtg aagaaagcaa
    12421 aatcttatta tgaacaaggt taccgatatg tggtggacat tgatatgaaa gcctactttg
    12481 acaccgtaaa tcacgataag ctgatgtatt tcattgagcg gaaagtaaag gacaaacaag
    12541 tcctaaaact gatccgtcaa tacttaatga gtggtgtaat ggaaaatgga ctcatcacaa
    12601 caactgaaga aggaacgccc caaggaggca atctctctcc cctgttaagt aacatctacc
    12661 tgaacgaatt tgatcaagtg ctagaaaatc taggacataa atttgttcga tacgccgatg
    12721 actgcaatat ctatgttaga agcaaacgag caggacagcg tgtgatgaag aaatccattc
    12781 aatttctcga agaacaacta aagttgacgg taaatcgaga aaagagtgcg gtaggaagtc
    12841 cattgaaacg aaagtttctt ggattctgca ttctgccaac aaagaaaggt attaaaattc
    12901 gccctcacgc aaaagcaaaa cagcgtgtga aaagcaagct caaacaactc actaaaagaa
    12961 accgtggaag aagtatccaa cttattttaa aagaaatcaa gcaactaatg actggttgga
    13021 tcaactacta tggaattggt gagatgaaag aattcatgag ggagttaaac ggttggttga
    13081 agcgaagaat cagacaatat atttggaaac aatggaagaa tccaagaacc aaaagaaaaa
    13141 atctgattcg tttag
//
LOCUS       DANKHL010000073        13083 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000073 DANKHL010000000
VERSION     DANKHL010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.076"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(78..2198)
                     /locus_tag="PW101_001468"
     CDS             complement(78..2198)
                     /locus_tag="PW101_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2513872.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2428..3606
                     /locus_tag="PW101_001469"
     CDS             2428..3606
                     /locus_tag="PW101_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDL2513873.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3622..4380
                     /gene="aroD"
                     /locus_tag="PW101_001470"
     CDS             3622..4380
                     /gene="aroD"
                     /locus_tag="PW101_001470"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDL2513874.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4403..4888
                     /gene="ybaK"
                     /locus_tag="PW101_001471"
     CDS             4403..4888
                     /gene="ybaK"
                     /locus_tag="PW101_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDL2513875.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4959..6212)
                     /locus_tag="PW101_001472"
     CDS             complement(4959..6212)
                     /locus_tag="PW101_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2513876.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6328..7677
                     /gene="gdhA"
                     /locus_tag="PW101_001473"
     CDS             6328..7677
                     /gene="gdhA"
                     /locus_tag="PW101_001473"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDL2513877.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7789..9135
                     /locus_tag="PW101_001474"
     CDS             7789..9135
                     /locus_tag="PW101_001474"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDL2513878.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9443..9829)
                     /locus_tag="PW101_001475"
     CDS             complement(9443..9829)
                     /locus_tag="PW101_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2513879.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10097..10246)
                     /locus_tag="PW101_001476"
     CDS             complement(10097..10246)
                     /locus_tag="PW101_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513880.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10708..10920
                     /locus_tag="PW101_001477"
     CDS             10708..10920
                     /locus_tag="PW101_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513881.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11198..12997)
                     /locus_tag="PW101_001478"
     CDS             complement(11198..12997)
                     /locus_tag="PW101_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL2513882.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4290 a         2353 c         2577 g         3863 t
ORIGIN      
        1 tattctaaga tttctcttta ccaacatcat ttgacaaaga gcctaatttt gtcgctgctc
       61 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
      121 gtttgcgttt tataaagatc aacagttgat tgtcccttgt gttcactaaa gacactagtt
      181 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
      241 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
      301 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
      361 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
      421 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
      481 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
      541 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
      601 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
      661 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
      721 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
      781 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
      841 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
      901 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
      961 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     1021 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     1081 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     1141 ctagtgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     1201 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     1261 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     1321 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt caagctggag accattctca
     1381 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     1441 ttttttcctt tagcgagccc gaacaaatca tcatttggtg ctgcataatg ttcttttaca
     1501 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     1561 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     1621 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     1681 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     1741 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     1801 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     1861 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     1921 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     1981 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     2041 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     2101 aaataggcaa aaatcgtttt caaccaaagt aaaacaacta gtaaagaaaa aaatccgagc
     2161 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     2221 cttaatcaca gaagtgtcat gaaaccatgt tacacagctc acttattctc atttatttta
     2281 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     2341 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     2401 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     2461 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     2521 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     2581 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     2641 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     2701 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     2761 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     2821 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcgtagg aggctatgag
     2881 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     2941 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     3001 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     3061 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     3121 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     3181 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     3241 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     3301 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     3361 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     3421 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     3481 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     3541 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     3601 cattagaagg agcgatgcag aatggtttgg gtaaagggat tgaatatagg aagcggtaga
     3661 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     3721 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
     3781 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
     3841 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
     3901 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
     3961 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
     4021 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
     4081 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
     4141 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
     4201 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggctggc
     4261 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
     4321 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
     4381 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
     4441 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
     4501 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcata tatttaaaac
     4561 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
     4621 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
     4681 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
     4741 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
     4801 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
     4861 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
     4921 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
     4981 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
     5041 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
     5101 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
     5161 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
     5221 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
     5281 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
     5341 cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga
     5401 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
     5461 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
     5521 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
     5581 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
     5641 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
     5701 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
     5761 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
     5821 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
     5881 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt
     5941 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
     6001 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
     6061 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
     6121 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
     6181 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
     6241 tctgaagaaa gaaaacagaa aaaatgtttt gagccaaaag agaaaatgag ctaaaatgag
     6301 ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt tcaagcaatt
     6361 caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc aatcgatgaa
     6421 ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga aaagaatatc
     6481 ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg gcaagatgat
     6541 gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc tatcggccct
     6601 tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa atttttggct
     6661 tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg aaaaggcgga
     6721 agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg tcaaagtttc
     6781 atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg agatatcggt
     6841 gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa aaaatatgat
     6901 actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg tacggaagct
     6961 actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa agattcattt
     7021 aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc aatcgaaaaa
     7081 gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta catctatgat
     7141 ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag acaacgtttg
     7201 acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc tgtttggaca
     7261 ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat agacgaaagc
     7321 ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc taatatgcca
     7381 tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg tccaggaaaa
     7441 gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa cgcccagcga
     7501 ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga aacgatttac
     7561 catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct tcttggagca
     7621 aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt agtttgaaaa
     7681 atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa ataataaaaa
     7741 aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat ggcacacatt
     7801 cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg gtacatgcaa
     7861 agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg aagcgacttc
     7921 cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg tatcaaagaa
     7981 gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat cggtggttct
     8041 tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa tttattagat
     8101 agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag ctcaacatat
     8161 attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt tatttcaaaa
     8221 tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct tcttgttaag
     8281 aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa agcaaaaggt
     8341 gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc tgatgacatc
     8401 ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt tagtggtgca
     8461 gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc aagcgacaaa
     8521 ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta ccgtaaaggc
     8581 aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc agaatggtgg
     8641 aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc ttcaagcgca
     8701 aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg tcgcaatatt
     8761 tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc tgaacaagca
     8821 gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt aaatacaaaa
     8881 gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt gctagtcaaa
     8941 attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga aattgctgta
     9001 ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt agaagcttac
     9061 aagaaaaaca tgttcgcact tctaggcaaa ccgggctttg aagatcttgc taaagaattg
     9121 aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg atagaaacat
     9181 gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg catcgtttat
     9241 tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat agaaatcgat
     9301 tgattgcctt gctctttagt ttgttttagg actttaagaa cagtgaggct gacttcgaag
     9361 gttaattctg tttttttgtt ttctagaatc agtaagcaaa aaagcactca cttcgagtgc
     9421 ttttttgcga tatattctta gcttaagata ggcctaattt ttcttttaca gatgagggta
     9481 ccttatcgct ggtaattact tttttctcta tgatccgctt ttcgcttgct ttcaactgca
     9541 agatcgttcc ttcggtgtaa gcagaaccat tggcagagtc accagaagta atgacatcga
     9601 aatctaactg tcttttttca ccgttttcag cataagcatc tacgttgtat ttaaaacctc
     9661 ttccaattac ttccccatta tctgctttga tatcagtttc tgcaggcatt ggagctttga
     9721 ctaccgcata ataatcttta cctacataag tagactgata gtagtcccaa cctttccatc
     9781 ctataaaagc aatcaatatg ataacgatca accctaaaat ttttttcatg aaagatctcc
     9841 ttttttattt ttattatatt aaatattata cagataataa cggaatatta tcatcgaaac
     9901 agctaacaaa attgtcataa aaaacgagat ggttgatatt agaacttgtg tttttgttta
     9961 ggtctagaga taaagagaga caatttccaa atatatgtaa tgaatacaaa aagacgtttg
    10021 tcgcggatta ccgttacaaa cgtcttttat tgattttccg agatctcatc agtcgccatt
    10081 tggaatggtt cactccttat ctagattcgt ttaatgctcg gactgcttgt tcgattgtaa
    10141 taccaaacgc aacgttattt tcgttttgtg catcaatcgc cataaaacgg ttagaagttg
    10201 tatcaaaagc tacgcggtat tgcatatttg tttttgtaaa cgtcataaaa ttcatctcct
    10261 ttgtaatgta atttagttct tgaaagtttc ctttaaactt tcgaacttat accaagttta
    10321 gcatcgtacg ttttttgatg caattaaaaa gcatttatca ttttccttat ttattttttc
    10381 ttaaatggtt tagctatttt cttttaatcc gataagtagc ccaatcatca aggttacaaa
    10441 tactattgct tgaatataaa tcatatgctc acctctttat aaataactta gcaatataaa
    10501 gaaaaaaacg cacgaacaac ataggaatat aaacaaacgt ttttgtcaca acgtctaaga
    10561 tcgtgttaaa aaatagttat ctaaaaagca gaaagatact caaaataaaa aataaaaaaa
    10621 tgaataaaaa cgttttcaaa tacaaggtaa tttgttatta taaaataaag aaaaaacaaa
    10681 ataaaaataa agaaaagagg gaatgaaatg gtaaaagcag aagtaggaag atcttatata
    10741 tgcaaaccga tcggagtatc tcatgaacta atcggctgta ttgaacgtac atatatcaac
    10801 acggctgtcg tgtatgttga aagttatgac caacgagact atcaacttat caatgaaagc
    10861 aagtatcgca tgctcgtaaa attctgtgat attagcgcac ctactgaatt ggtttcttaa
    10921 acagtatttt tgagaattcg caagaactcc cttataatat aaataaagac agaagggagc
    10981 gagaagatat gccaaaaaga aaaagttcag ggaaaatggg aattattctt gcagtgatct
    11041 ctgcaattcc tgctatttta gctattgttc gtgcgatgaa ggaatcaaga gaaagttaag
    11101 cacacattat gagcgtcaaa caaaaaacgc atatacctgc ggtggcaggt tatgcgtttt
    11161 ttgttgtata aagacttttt tcattaacga tttttgatta gccgactccc aaaacaaaat
    11221 ttagcagttt atctgaataa gtgatttctt catctgtttg tttcacactt tcatttagta
    11281 gtcccatctc atcggttatg ataccatcca caccataaaa catcatgcga ctcatggcat
    11341 ctgaatcgtt gattgtccat acatatactt cttttccgtc ttggtgggca gaattaatga
    11401 agttacggtt gatcgtacta tattccattg tcagaaaatc agcaggcgtc acagggggcc
    11461 caacaatatt gaaaggtaag atgtagccca cataaaagtt tggttctgct tcttttaaat
    11521 ctgaaacggt ttggtacgtc aaactttgca aaatatgatg gttctctaag ataactttgc
    11581 ggtatttatg gacaaatcgt tcgacaaggt catccgaatc ttttttagag gtcttgatct
    11641 caataagtaa tttttggttt agcttatgtg ctttttccaa ataagaatca aaagaacaaa
    11701 caggggcttg cactccgttt tcagtaactg taagatttgt caattctttc aatgtaagat
    11761 cctgtggtgt tccatttacc cctgttaagt gacgaagatt tgcatcatgc atcactacga
    11821 aatgaccatc ttttgtttcc tgaacatcca tctctacata atcaggatga tagtctctgc
    11881 ttgttttttc aagtgcttct aaagtatttt gcgccccatt ttgggtactc acacctctgt
    11941 gagagacagt cactggtttt gtctgaacag ctgaagtcag ataatccata ttgtaaagac
    12001 agacgccaat accgaaaaaa actgcaaaaa gagtcaaacc agtgtttttc agcgcagaga
    12061 aacgcaagtt gggggcttgt gggataaacc acttagggat ctcaggcaag aagccttcgt
    12121 catccatgaa atcaacaatg atataaaaaa tccctacggt agacatcacg atattgaata
    12181 ataaaatccc ttgtaaaagc gtcatagcaa acacagcgct gatcaatgca tagtctggga
    12241 aaaattgttc aaccatactt tgtgccaaaa taacaacgat atatcctaaa gaagaaagga
    12301 gtaaaatcgt accgccaatc acaataaatt ggcccgtgat ggccaaaaga cgcgatttgg
    12361 tcaatgacca gctttcccga atggctgctc gaaaagaacg atctcgtaga atcatttccg
    12421 gtaaagcaaa aatcagtcga atcccaatat agccaaggaa aatataaacg agtaaaaagg
    12481 aactgacgat gatccaacga ttagcaaaaa taaaatccat aatgaaagcg gggattttta
    12541 tttttgacag tagatcagaa ttaaaactta atccgctgat cggtagaatc aataaaaaat
    12601 aagcaaggaa aaacaaaaaa gtaatgggtc tcactttctt cagctgaagt atcgtcaagt
    12661 gtaagagttg cttcaaagag attggttctt tctttttaat aaaaaaaaca ctcatcaaaa
    12721 gaaaagtaaa ttcgaaataa actaacagta aaatcgtcat caaaactacg attaacgaga
    12781 gtagaacccc aggatgttga gaaaaaattg aaggaatatt gtcataagac aaatagtcaa
    12841 ttgcccctcg gtgtaaaata aaacgggtcg tactgctcaa taagggtaaa caaataaata
    12901 aaataaagcc atgcatcagc aagacatcac ggaaataagc ttgtgttcct tttaaaaagt
    12961 cccaagcatt acctaagcta cgtttcaaat aattcatcct atcacctgct cttcacattg
    13021 tagcgcaaat ctaatgaaaa aggaaagggt gcagtgtgtt ttgcagaccc tttcccagat
    13081 tta
//
LOCUS       DANKHL010000074        12981 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000074 DANKHL010000000
VERSION     DANKHL010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.077"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(274..993)
                     /locus_tag="PW101_001479"
     CDS             complement(274..993)
                     /locus_tag="PW101_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL2513883.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1042..2688)
                     /locus_tag="PW101_001480"
     CDS             complement(1042..2688)
                     /locus_tag="PW101_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2513884.1"
                     /translation="MDNQRKPSLDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            2867..3457
                     /locus_tag="PW101_001481"
     CDS             2867..3457
                     /locus_tag="PW101_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2513885.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3549..5054
                     /locus_tag="PW101_001482"
     CDS             3549..5054
                     /locus_tag="PW101_001482"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDL2513886.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5138..6490)
                     /gene="celB"
                     /locus_tag="PW101_001483"
     CDS             complement(5138..6490)
                     /gene="celB"
                     /locus_tag="PW101_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL2513887.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6490..7008)
                     /locus_tag="PW101_001484"
     CDS             complement(6490..7008)
                     /locus_tag="PW101_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513888.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7024..7350)
                     /locus_tag="PW101_001485"
     CDS             complement(7024..7350)
                     /locus_tag="PW101_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HDL2513889.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7363..9342)
                     /locus_tag="PW101_001486"
     CDS             complement(7363..9342)
                     /locus_tag="PW101_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2513890.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9363..9677)
                     /locus_tag="PW101_001487"
     CDS             complement(9363..9677)
                     /locus_tag="PW101_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HDL2513891.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            9845..10138
                     /locus_tag="PW101_001488"
     CDS             9845..10138
                     /locus_tag="PW101_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDL2513892.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10216..11328)
                     /locus_tag="PW101_001489"
     CDS             complement(10216..11328)
                     /locus_tag="PW101_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2513893.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11481..11705
                     /locus_tag="PW101_001490"
     CDS             11481..11705
                     /locus_tag="PW101_001490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513894.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            11715..11894
                     /locus_tag="PW101_001491"
     CDS             11715..11894
                     /locus_tag="PW101_001491"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513895.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12088..12258
                     /locus_tag="PW101_001492"
     CDS             12088..12258
                     /locus_tag="PW101_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2513896.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(12682..>12981)
                     /locus_tag="PW101_001493"
     CDS             complement(12682..>12981)
                     /locus_tag="PW101_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2513897.1"
                     /translation="DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4093 a         2612 c         2206 g         4070 t
ORIGIN      
        1 acaccgttta tcagaggttg tgaattcagc gttttggcct gagtattagg gagatcgtgt
       61 ccttactatc taactctgag tcacaaggaa caatggggac tgtaacaaaa gtcttgtcac
      121 agtcccttat tccgaacaaa cggtgttcca gaagtagctt ctacggaaat aagccgaaat
      181 ttcacaaaaa agccatccga aagtcagacg gacaagtctg cttttcagat ggcgctacaa
      241 ctactctgct tattctttta gctatccatt caattatttt tctctcactt gttctagctc
      301 ttctttggtc atgggtcgat attgcccttt attcaaatct ttaggcagcc aaaattttcc
      361 catacggatt ctttttaaat ataagacttc ttttcctact gctttgacca ttcgcttaac
      421 ttggtgaaac tttccttcat gcaaaatcag acggataatt gaagtttctg tctcttgatc
      481 tacttgatca atttggagtt ttgccggcaa tgtctgctca tcgccatcaa taatcagtcc
      541 ttctgcaaat aaacggatat cttcttctgt cattataccg ctaacctcag ctagatattc
      601 tttctccaca tgttttttag gtgatagtaa ctgatgggca aacaccccgt cattcgttaa
      661 aatcaagagg ccctctgtgt ctttatctaa tcggccgact ggaaaaagat cttcccgata
      721 atcctggtct tccaataaat cgattacggt ttgatcatga ttatcctgag tagcagaaat
      781 gactcctgca ggtttatgca aaatgtagta aaaatctttt tgatacgtaa tgatttctcc
      841 taaataagta accgcatctt cgaattcatt tacttgcgct ttatcgcttt tcaccacttg
      901 tccattgact tcgatcgttc cttttttgat taatcctttg acttctttgc ggctgccaag
      961 tcccatatct gctaaaaact tatctaaacg catgcgtttc ccccatttca aaagagcatc
     1021 aatctagctc ttctgattca cttattttat ttttaattta tgtcgtaaac gcgctattcc
     1081 tggtcctagt agtctttctg ccaagcggat tttcaaagct aaataaccat acactaaagc
     1141 ccctatacct gctactacaa gaatcaacaa tagagactgg aatttgctat cttcgtttaa
     1201 aaaagcacca aaaatcatct ttgtgatcca cgaagccaaa agcataatca aggtaagaat
     1261 aaagatcagc aatgtcctgc gccagacaaa acttggttta aagcgggcta cttcatggat
     1321 tctctttaag atcaaggtac aagacacagc aaatccgatc attgtcgcca ataaagggcc
     1381 gtatacttca aataaacgaa tggctgggta ttgaagaagg agtttcacta gaaacccaat
     1441 aaccaaataa ttaattgccg ccttgttttc aaacattcct tgcagcatgt tggaaactag
     1501 catatatagt cctaggaaga gtcctacaaa acttgcttgg atcagtacat tacttcctag
     1561 agaatccggt gtataaaata atgtatttag cggataggca agtaacataa caccaaatgt
     1621 cgccggaaac ataaaaaatg aaaacaactg taaatttccg cttgtcaatt ttgccagacc
     1681 tactcgatct ttcaacgtaa cagcttctgt aatcaacggt aacccagtag acgcgatcga
     1741 tgttgccaat gcaataacta ccatcgtcaa cttatctgga ttggcactaa aaatcgaaaa
     1801 aagatccagt aactgtgcat tggaatactc ggtggatgca gacatgatct tcataaaagt
     1861 gaactgatcg actaatttga agatcgttac ccctgaaccc acaataataa acggaatcgc
     1921 ttctttaacg gtatctaaca acaattcctt cgttgcaata tttattttgt tttcgctgta
     1981 gtgaataaac gccgatgtat acgcttgatg tttcttcaaa aaatacaaaa ggacaagaat
     2041 acttgctagc ataccgataa atgccgcgaa agttgattgc gtgaccgccg tcacatagtc
     2101 accatccatg actttcatga tgatgaatgt agccaatagc atataaaaaa tacgtgccac
     2161 ctgttctaca atctgcgaca gcgcataagg catcatttct tgattgcctt gcaaataccc
     2221 tcgtatcaca ctcatacaag gaaaaaccaa tacagcgata ctcaaagaac gcatcgttgg
     2281 aatcagttct tctccgccac cagaagcatg agctagccaa ggagaggcga tgtacatgaa
     2341 caaagcaaac aacaggccta acccgcccat catctgtaat gcacggataa ataatcgtcg
     2401 tgaagtgcca tattcattca atgaattata gcgtgcagtt tgttttgcaa tcgcagcagg
     2461 tattcctgct gtagagatca gtagaaacaa tgcatatata ttgtatccca tattgaataa
     2521 tccattggca gccttagcat tttcgcccat ccaagcatac cagggaatga tatagacagc
     2581 gcctagcaat cttgaaatga tattactggc ggtcatccaa gcagagcctc gtgccatttt
     2641 ttcttgcggt gttaattgat ctaatgatgg ttttctttga ttatccatcg gtcagattcc
     2701 aactttctac aaaacattac tatcatttta attggtaaaa cagaaactgg caatcgattt
     2761 tatataactt ttataagaac aattgttttt ttgaattcgg tttcgttttt ttggtaaagt
     2821 aaagaaatat acagcctttt atggctaaca tttataggag atatcaatga cacaagaaat
     2881 aaaaaaacta cccgttatat ttttaggtct tttctttatc acagttggat tgaattggtt
     2941 cttgctccct cacgatatag cttccgccgg agtgggcgcc atcggacatt tagttgaaac
     3001 ggccttttct atccaacgct cagttactgt ttggagcatc aatcttatga tgctgcttct
     3061 agccgctgtc ctattaggaa aatcagtatt ttttaagact gtagcgggaa gtctgctttt
     3121 tcctgttgtt ttagagattg ttccaaaagt ggaaatgatc tcctcacatt tcttttcact
     3181 gatttcagga agtcttttgt tcagtcttgg ggtttacaca ttgtacacag tcggggcctc
     3241 aaatggcggt ataacgatac cgccgattat tttccacaaa ttttttcggc ttccaatgcc
     3301 gcaaggactt ttacttacca atagtcttat cgtctttttg aattattatg tttttggatt
     3361 gttggagaca ctattcgttc tcctatctat tacagtcagt tcattgttta tgaaaatact
     3421 catccgcttg tcccctgtgt caagaaaatt tgaataaagc tggattttgt catagcggaa
     3481 acaaacgata tattaaaaag agtgtgctat aataaaccaa gaaattgaat aaagcgaggg
     3541 atcaatcaat gagcttctct ttagaagaca tccgtcattt actattaaaa gaaaatctat
     3601 taaaagaatt tgtttcaaca agaggctggc atcttgatgt accagaaaaa gctgaatttc
     3661 ttcagttaag ttatgattca agaaaagctg atgcttctac tttgtttttc tgtaaaggaa
     3721 tgaatttcaa agaagaatat ttggattcag caatcgaaca gggtattcaa tattatgtat
     3781 cagaacaacc atacgaaaat tcagcagtag gaatcatcgt aacggatatc cgtaaagcaa
     3841 tggcgcttct tgctatggct ttttacgact atccgcaaaa taaattgaaa gtgatcggat
     3901 tcactggaac aaaaggtaaa acaactgctg cgtactttac aaaagcaatc ttggatcaca
     3961 caacaaataa aaaaacagca ttgctttcta ctatgaatac aacgttagat ggcaaaacat
     4021 atttcaaatc ccatttgaca acgcctgagt cattagattt atatcgtatg atgtcagaag
     4081 cagttgaaaa tggtatgact catctagtga tggaagtttc ttcacaagct tacaaaacac
     4141 aacgcgtata cggcttgact ttagatgtcg gaatcttctt gaatatctcg cctgaccata
     4201 tcagtccgat cgaacatccg acatttgatg actatttcta ttgcaagcga caattgatct
     4261 tgaattcaaa aacagttgtt ttaaatcatg aaagtgacta ttttgattta cttagggaaa
     4321 cagccgatct attccagatt ccaacgatca cttatggtcg ttcggaagaa agcaattatc
     4381 aagtgatccg ttcagataaa ggtactcacg gttttacttt atcttctcat gaagatcgct
     4441 tagctatctg cgatactgct tatgatattt tgctggctgg aggattcaac caagaaaatg
     4501 cagccagtgc gatcattgcc gcagcactaa caggtgcctc cagtcaagat gcacaagacg
     4561 gattaaaaga agctcgtgta ccaggccgaa tggatcaatt gatccaatcc aatggagcgc
     4621 atgtctatgt ggactatgca cataattatc taagcttgaa aacattgctg aaatttgcca
     4681 aaaatgaaca tccagatggc cgtgtaattg ttgtattagg cagcccagga aacaaagcaa
     4741 tctcccgtcg acatgatttt ggaaaagtgc tatcagaaac agctgacatc gcctttttga
     4801 cggcagatga cccagcattt gaagatccga aaaaaatcgc ggaagaaatc aatgaagcaa
     4861 ttactaatcc agatttgatc gttcattatg aaatggatcg tcctgaagcg attcgacgcg
     4921 ctttagcaga aagcgctccg caagattcag tcgttattgc gggaaaaggc gtggatccct
     4981 atcaaaaaat caatggtgta gatgaacctt atgaagggga ttatgcaatc gtgaagcggt
     5041 tgattgaaga ataatggtat ttatatacta gtggtttccc atttctaaga aaaaaaggga
     5101 ctgtgatttt ttgtcacagt tccttttttc ttagaaatca ttttaacttt tcactagcta
     5161 attctgctgc cttttctctt tcttcattta ataatttatt atcatagaat ttgataaatg
     5221 ggaaccagat aatgaatgcg atgattgcac aaatgactgc taacacagca gctttccaat
     5281 caccaccact accaacaaat gctccgatcc cgactggtga aggccacggc atctgtgcaa
     5341 gcatcggttt gacgatttcc agccgaatgg caaaataagc gagtgtagca gaagccatcg
     5401 gagctaagaa aaacggaacc gctaaatagg gattgtagac gattggcatc ccgaaaatga
     5461 ttggttcatt gatattgaat aatcctggta caagtgaggc ttttcctaac actttcagct
     5521 gatcagattt cgcgaggaat gcaataaaga tgatcaatcc taaagtcgca cctgatccac
     5581 caacggttac ataagaattg ttgaattctc ctgccaacgg ataatttgcg ccttgtgcgt
     5641 tagctgccat attagctaat acgatcgggg tcaagaaaga agtgatgata ttcgctccat
     5701 ggatccctac gatccacaat gcatggatca agaaataaat caccatgaca ccaagccatg
     5761 tattcgtcaa ctgcgtaaca aacccaaatg ggatagcaat cattttgaag atatccgttc
     5821 ctagtgcaac taataatcca ttgatgatca tcaccacaaa cgcaatgaca aatgttggga
     5881 tcagagcagt gaaagaacga gaaactcctg gcggaacaac atctggcatt tttatgaccc
     5941 aatttctctt gacacacata caatacaatt cgaccgcaat aatcgacata ataatagctg
     6001 taaagattcc tgaagttcct aaacgttcga caaaagagcc catcctaata ccatttacta
     6061 cagtttctgt ctcattgata gaagcaatca agctgatttt accatcagtc atgatcaatt
     6121 cagggataca cataaagaat gcaaaaacag atagtaatgc ccccgtgagc ggattaacat
     6181 ttaatttttg ttcatctcgt tcaattgacg ttaattcata agcaaacaca atggcaaagt
     6241 aaatagataa aatccccatc gtcattgtat tgacgatcat gtacaagtca ctaactttga
     6301 aaaaagttgc attaaaaaat ccttctaaaa atgggaacgg taatggcaaa atatttaaaa
     6361 ccaaaaacat cgagcctaca atcgtgaaag gaatagaagc catccccgct gccatgacag
     6421 cccgaacgat tttaagttgc gcaattttcc ccataggtcc catcaggtat ttctgcaaaa
     6481 aatcaaacat tagttattct cctttcctaa atataacgct ttttcatatt gtttaatacg
     6541 gatatactgt tggtcttggt tgagtgaaat tttctttaaa cgatactgta taaatgcact
     6601 ggcaaaaata ccgcttacaa caagaatgaa aacaaataac tgtcctccat tgttatttgc
     6661 cataaaagga tacaagaatg taaatcctga ggtaaacaac actggaatca ctaataaatt
     6721 aaagacactc tggaacaaaa aataccactt tgtccatgga acgttcgttt ggtgttttcg
     6781 ataaagcttt acttgttctc ctactgcagg caaaatagct aaaatcagaa tcatcggtaa
     6841 actacttcct aaacttttag ctgatagaag cagaatcatc cattgcatgt tcatgaaaaa
     6901 gaaaaaggca gtcaggtaac gtacgattag ataacgattg aaatacatgt ttttcaaact
     6961 aagttgcttt ttttctaatt cgattttcct ttttactgta tcctgcacaa tgatccttcc
     7021 tttctattta tttttttgat acaaataatt catttcctgt gcaacttcca ataatgtcat
     7081 cgtcgtcatc agatgatctt gtgcatggac catgataatt tccatttcta tttttgtccc
     7141 gctggcatat tctttcagaa gttgagtttg tgactgatgc gcatgaagaa tctcttcatt
     7201 tgcttgtcgc aacttctctt ctacttcttc aaacatctct ttacgcatcg agcgaaatgc
     7261 ttcatgtatc agtgtccgtg catttccgct atggaaaatg atatcaaaag aagtcacttg
     7321 tagtgtttca ctgtttattt tttcagccat ctgttttcct ccttattcga tcagtttgat
     7381 aaatttactg ctgaaattct caaaaatagg ttcagctaag atttcctgct gtacttttag
     7441 ctggtcaact aacctaacga ttgctttagt cacgaccgta attccttcat tttcgacata
     7501 agaaggcgaa actaaaaaaa caaaatgaat ttcttgatat tgatcccact gcatcccaga
     7561 aggaatcaat gcaacggcta cctttgtttc gattccaact ggaacagcgg gatgtgggac
     7621 tgccactgta tcactgaata tgatttgtcc catattttca cgttgctgga tctgctttcg
     7681 catttctctt atatatcctt cttcttcatt tatgctcagc ttttgcagca aatcatgaag
     7741 aatttcttcc tttttcggtt cgtacgtata agtcttgaaa aatttttcag acaactgttc
     7801 ggtgtaaatc tgcttttttt gctgtagaga aaatgctggt atcggtctat ctgaggaatg
     7861 atagggtata gattctccaa ctacctttcg tattttacga acatcatctt cattcaaaaa
     7921 aatactgaca tgtaaaactg gaattttgaa aaacattgtc gaaagatcaa tagaagaaat
     7981 aatcaggtcg attcctttta aagttgtttc attgatctca taatagcctt ttacacttac
     8041 cactgtaata tttttaccga attcactgac tacgcgattc tttaatagtt gggcactgcc
     8101 gtaacccgtt gcacagataa tcaatgcatg tagtttatgc gcagctcttt ctttttccaa
     8161 tgccgccatg agatgcaaag ccaaataggc ccattcatct tcattgatgg tatagttttt
     8221 caaagccggc atgttgctga aatgcctttt tactgcgtca aagactttgg gattttcttt
     8281 ctttatctcc tcagtcaatg ggttctcaat gcgaatacct cggctcaatc gaatcatcat
     8341 cggtgacaaa tgatcgagta gtccattgaa aagctggaga tcctctacta atgaaaaatg
     8401 aaattcctga gacaatcttt tcagaacttt cgctaattcc tttgatagtt cctgatcatt
     8461 ttgattttct ttgtgattcg acttagccat gagatgtaat gtcaaataag caatttcttc
     8521 ttcgggaaac gatacacctg cgattacctc aatacgttga acgatccgct gcgccacttg
     8581 atactctact cgcttagaaa cttcttgttc gatgcctaat tccttgattt caaacccttc
     8641 tcggagccgt ttgatcccca aagccaagtg taaagtcaga ttctgtatga taaaatccga
     8701 aacttttaac ttagcttctc ttgtttcatc gagaatgata atgactagct cttcaaaact
     8761 gatgtctggg aaaaaacggc tttgccctaa atattctttc aatgaatttc cataatggtt
     8821 tccaaaaaaa gtatccatga taaaatgccg tcgatttctt tcttcccctt cgatccatgt
     8881 cccttgacca tgcttagaaa cgatttttag tgaatatgga atcaatcgct ccttgatttc
     8941 ttgcatgtcc tttgtcaaac tggaacgact gatataaagc tcttctgcca aatcatccat
     9001 aaaaagaatg gcatcttcca aaagcaattg gttcaatata tacttttgtc gatcttcgga
     9061 ctcataaaat ccttgcggtt ctctagcata tccaggtact atttcttgcc gactcaaaaa
     9121 caagtcaaat gtcagtcttt gtaaaatatg cagttggtag ccataacctg gtttagcaat
     9181 gatcttacct ccgttttttt ctactaactc tttgatccgc tgcaagtaat tacggattgt
     9241 ccgatcagat aataacaatt cagaagctaa acgttgactg gtgacatact ccttcttgtg
     9301 atcaatcaaa tatctcaaca gcaatttttc cttctgtttc attatcatcc actcctattc
     9361 catcatatgt tctcttcgat cacctctgct aacttctgga tgcctgtagg gattgggaca
     9421 tacgcttgga aagggatact aacaacaggc tttccaacag attctcctaa ttttttgaat
     9481 ttatccaaaa acatcgtcgt ttgcgggctg atcagaaata aatcaaaatc actattctta
     9541 atcatattat cgccttctgt agctgaaact gcatctacga gtacctcttc tcttttttgc
     9601 ttaaagaaat ccgttgtctt tgaggccatc atcgaggatg acattcctgc cgcacaaata
     9661 atcaatgctt ttttcataaa tcaatcactc cttttatttt tataaaccaa gcatatcttg
     9721 taagcgtttt ctattcaaaa tatatatttc cgtagcattg cggaaaaaat gtgtatcata
     9781 tcttgaataa taatttctgg caccgtgata tactagtgat agaatttttt caggagggga
     9841 tttagtgagt atcacagtga atattttgta tacaagtaca aatggacaag ccagaaaatt
     9901 tgcagaagaa atggtcgaaa gaaacattgt caaagcgatt cgtgcagaag aaggaaacga
     9961 gaaatatgac tattactttc cactagaaga tccagaatct cttttactga tcgaccgttg
    10021 gaaagatgaa gaagcaatcg aaaagcatca caaatccgaa atgatggctc aaataaacga
    10081 actccgtaaa aagtataaat taaaaatgaa agtcgaacgt ttcactgatt taccgtaaaa
    10141 agatacacat ggaatgatag caatttatca tccatgtgta tcttcacttt ttccttattt
    10201 tttattcgat ctcatctaat gattcaaacc gtgtttttct cttatttttc ggataagtat
    10261 ttcagtcacc ccaatataca tgattcctaa acatacccca aggatattct caaaaatgcg
    10321 gatcgttact gctcctggaa ccccgaataa actggcagtg attgccaaag caccaaaaga
    10381 attaaaaact gttttatatc tataggtaga acaaacacct aaagctagac cacctaatat
    10441 accgacccag ttaaaaggaa taaattggct gataccaata aataaaacac atccaatcaa
    10501 tgagccaatg attcggtcta ctgctcgttc tttgaaacca atcgacatca atccatagct
    10561 agatacaata gaagaaaatg caaaccctgc ccacatcaat cgctgaaaag ggacatactc
    10621 tcctgcaacg atcaaaagac taatgccaaa tgcatagatg agcatccaaa ttttttcctg
    10681 cgaatacatg cctttcaaaa aaatctcttt aaacaagctt ttgtctttgt tcttttctcg
    10741 atgttttctg taaaggagaa ctgaaaacca acaaaaaaac aaaaataata agctacttgt
    10801 ttgtgtaaag aaattcttgt tcaacgaatc tttaggtaac gaataaacaa taaagagata
    10861 tgaaaagccg tacaaagaag cattccccat tttgggatca ctagcggttg ccattaatat
    10921 gctgcttagt aaaataaaat gaatcaacat gcccataact gaccatttga tcagctggac
    10981 atacggtgct actaatagtg agaacatgac gattgctaaa cctatgatcg aatgggaaat
    11041 cttataacca aagtcaacaa aacgaatact catcaacata cagaataaaa ccacagcgta
    11101 tggtttatgc ttcgattcaa ataatgttgt aattataatg ataaaacaaa tggcaaacaa
    11161 tacgatgaat aatgatcgta aaaacaatga acaaatatac atgcactttt cttttttaga
    11221 agctgcttct cgtatttttt gttttaaaat aaacggatct agttgtagag actgataaaa
    11281 atccacctgt tgtcgtcctt tctttttact tttatttttt acagtcatct atgacaggtt
    11341 gtaaatacta tggaacatca tatggcgttg tcattttctt gtcaactatt tttcctattc
    11401 atgtttttat aaaaaaacat atcgttttct tgtatgatta aatcaccaca aaataaacaa
    11461 ctgagagaac gatatgcttt atgaatggtt ctatcaaaaa aattctgaga tcaatagaca
    11521 aaaatttgat gattacagag gtctcttacg aaaccttaca gaagaaaaaa acattgatcg
    11581 ttgctgcagt tctcttccct gctccatgtg tctgtagaaa ttgtggttct gctgtggtag
    11641 atggaaatag gaaattaatg gtggtgaaaa acaagaagaa ggaaaccatt gtccactttg
    11701 aataatataa tcatatgcca atggttatac gcctaaaaaa gcagcgctat acctgtaaaa
    11761 actgtcgaag ccattggaac gctcaaagtt attttatccg tccaaggcac tcgatttcaa
    11821 atcatgtcag acataaaatc acttctttac tgactgaaaa agtgtcgtta ttttttattt
    11881 cgaaaagttg ttaggtatct ttgacaaccg ttattcgcac attgaaagag ttgagaagct
    11941 atttaccaaa gtcttccaaa agaattctgt cttgagtgtt gagggttgtg aatttcgttc
    12001 tcacgtttcc gttgaggata aactgagttt tatttccgcg gatggcgaga cagaaaaatt
    12061 aatcgagaaa atttacctac acaaaaaatt cctcgattaa caaactattt ccttagctgc
    12121 actaatccta aagaaataga attcttagtt acagacatga acgccaccta ctttcagctc
    12181 accaaacatg ttctaccaaa tgcgaaattg gtgattgatc gattacttgg tttttccacg
    12241 cctttcaagg aggcgtaacc tttattcacg agttggttga agctttccga gacaaaagac
    12301 ccgacttgtt tttctcctta ttgacaaaga gccattaaaa ctagagaaaa aattacaact
    12361 gatcgctgat tatttgtaga aagaaaatca aagtattacc caataaactt gagtcattag
    12421 gcaaaaaaca ctgcgaaact caagaatgat tttcgcagtg tttttgtata ttttttatta
    12481 actagtatta ctacaaaggt ttatttttca cttctcaacg cagcacaaaa ttgtctttac
    12541 ttatttactg gttgggttca accttctcta ctccgatact aatatttgtt tgtatacccc
    12601 ttctaaaatc ggtagtttcc attcaattga tgggtactag ttgattttaa aaactatgct
    12661 tattcaattt tttattttag ctcatatatt tcgtttcttt ctaaatttta tactgatgaa
    12721 aacaagtagc acacccaata ttgacaatat aaactggcta atgatggttt ctccagtttt
    12781 tggaagatgc ttatttccac tgcttgttgg attgtttcct cttatatctg agctatcagc
    12841 aacttttcta ttctcttttg ttaaaaagat cgccacttct tgttcttttt cgatcttgaa
    12901 agttactggt gtcgcatcca attcataccc cgttggcgct tgagtttcta ccagctgata
    12961 tgctcctggt gctaagccat c
//
LOCUS       DANKHL010000075        12889 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000075 DANKHL010000000
VERSION     DANKHL010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.078"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..1146)
                     /locus_tag="PW101_001494"
     CDS             complement(<1..1146)
                     /locus_tag="PW101_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2513898.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(1268..1864)
                     /locus_tag="PW101_001495"
     CDS             complement(1268..1864)
                     /locus_tag="PW101_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2513899.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(2064..4706)
                     /gene="alaS"
                     /locus_tag="PW101_001496"
     CDS             complement(2064..4706)
                     /gene="alaS"
                     /locus_tag="PW101_001496"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDL2513900.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            5094..5525
                     /locus_tag="PW101_001497"
     CDS             5094..5525
                     /locus_tag="PW101_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2513901.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(5570..6919)
                     /locus_tag="PW101_001498"
     CDS             complement(5570..6919)
                     /locus_tag="PW101_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2513902.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(6989..8152)
                     /locus_tag="PW101_001499"
     CDS             complement(6989..8152)
                     /locus_tag="PW101_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL2513903.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            8281..8604
                     /locus_tag="PW101_001500"
     CDS             8281..8604
                     /locus_tag="PW101_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513904.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(8787..10136)
                     /locus_tag="PW101_001501"
     CDS             complement(8787..10136)
                     /locus_tag="PW101_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2513905.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(10205..11158)
                     /locus_tag="PW101_001502"
     CDS             complement(10205..11158)
                     /locus_tag="PW101_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDL2513906.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(11260..12450)
                     /locus_tag="PW101_001503"
     CDS             complement(11260..12450)
                     /locus_tag="PW101_001503"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDL2513907.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         3706 a         2712 c         2226 g         4245 t
ORIGIN      
        1 atataaaaga taattgtgat caaacgtaat ttccaattgg taatctccga ttttttcttc
       61 tatttgacta cgtaagattc tttctagttc tgtatcaaca aactggaatt tctcagggac
      121 cactacatca aattgcagcg tttttccttg atgaattact ctaaaatcat gaatattaag
      181 ccctgtacct gtttcaagaa gaatttgctt taactgccgc aagatattat agtatttttc
      241 gttatcaaca ggcattggat ccaagtgaca gacaagttcc acatccaaag actttttaaa
      301 gtctttttct atcagatcaa taatcttatg agccacattc aaattcattg attcatctac
      361 ttcgatgtgg actgatgcaa agcgtttatt gggtccatag ttatgcacca ataaatcatg
      421 atagccaagt atatcatcgc aagcatttaa atgctcttcc atttcttcaa tctctgctgg
      481 cgttgggcga cttcctaata gttcgtatac aaaatttcga agcatcgtca atccactata
      541 aaggatatat aatgctaaag caaaacctat atatccatca atacgccatc cagttaccca
      601 ttcgattgct gctgaaagca atacagccag tgtagtaaag acatcattat aactatctgt
      661 tgctgtagct ctcaatgttt cagaatcaat ggattgcgca ataacctgat acattttgcc
      721 ttgtgcaaat ttcaaaaaga tggaaatgat taggacaaaa aagacgatgg gtgttaaaga
      781 gacattttct ggatggatga ttttttcagc agaagactct aaaaattgaa atcctacata
      841 agtaatgatg atcgaaacaa ataacccact gatatattcg aagcgttcgt gtccataagg
      901 atgttcctga tctgctggct ttgcagcaat tttaaatccg atcaaagtca atatggatga
      961 agcagtatct gatagactgt tcactgcatc agccataatg gagacacttc ctgaaaaaag
     1021 cccgatcatt agcttagaga caaacaagat cagattcgta accaatccta agatacccgc
     1081 tagtactcct ttttttgtcc gctgttgcgg tatttgtttc tttttttctt cttgtttctt
     1141 agccaagttt tttacccctt ctagtttgct tgttgctcag gtaaaaagat ggagaggacg
     1201 attgttttcc tctcctctct tcctttttct atctatcgtg cgtcctatta ctattaaaag
     1261 atggtcatta ttctggtttc aacggtgttg ctgcaaataa cgcttttgct agtaatggtg
     1321 ttatgattcc cgcacctatt gcttctggta tcccattgat tgctgcaacc cctgctaagg
     1381 ttttaaataa aagagcagag tctacaccat aggcatctgc cgttgcacct gtataaaata
     1441 atcccatgaa tcctaacact aagactgtat tgaccaatga acccagtacc cctgcaaagg
     1501 ctgagctgat cgttattgat ttattttttt gataaatcca gtggaaaacc aagcctgcta
     1561 ctaagccaac aagtactctg ggaactaccg agataatcgg attcgtgaac actaatgtat
     1621 caaatggcgt tgttggcatg gcataggcac gaacaattgt agcgattccc caaaacaagc
     1681 caatcaccat gccatctttt gggcctaata ctactgcagc aataattact gtgatatgga
     1741 taattgttaa actaatgaat cctaatggaa taaaccccag ccaaggaacc atcgcttgta
     1801 cgatgatgat tgctaacaag atcgcacgta agactagtcg aaaggtctta tttctactat
     1861 tcattttttc accccttttt atttatacta atcgtaaaaa tttctgtcac tttcgtcaac
     1921 taaagaccat ccgaatccga ccatttgatt agatgcgcca tccaattgaa aaaagtatga
     1981 gggcaagact tgtttgtctt cgcccccata atcatgtttc gttcaatgtt tgaacttttg
     2041 tttttctgtc aaacgtaaac gccctattgt tgcgcaaccc attctttcgc agctttaagt
     2101 gcatctggaa taccagcagg atttttcccg ccagcttgtg ccatgtctgg acgtccacca
     2161 ccgccaccgc ctactttcgg tgcgattgct ttaatcaagt cgccagcttt tagtccagct
     2221 tcatttgatt ctttcgtcat tgccgctagc agactgactt ttccttcttg ttcgtttgct
     2281 aaaacaagaa tatctgaaat atttttttgt ttccattggt cagctaattg acgcagctga
     2341 ttcatatctt tcacttttac ttgtgctgca atgattgttt ttccattgat cgtttctgga
     2401 tctttgaata catcaccagc ttgttgattc gctaacttgc tagctaattg ttcattttcc
     2461 ttttgtagct ctcttacttg ctgttgcaat tgttccgctt ttgaaacaac ttctttcaat
     2521 tgaggtgatt taacaattgt tgctacttgt tttagttgtt tttcttcttc ttcaagtagt
     2581 tcgtacgctt ctttacttgt tacagcctcg attcgtcgca cgcctgcacc aattcctgat
     2641 tcagaaacga ttttgaagat tccgatgttt tctgtatttg ctacatgtgt gcctccgcac
     2701 agttcgattg accagccgcc aatatttact actcgaacgt catgtccgta tttttcacca
     2761 aacaaagcca ttgcacccat gtttttggct gtatcgatat ctgtttcaat tgtttcaact
     2821 gggatagctt cccagatttt ttcgttgacg attctttcca tttcagctaa ttcatctgaa
     2881 gtgatttgac caaagtgggt aaagtcgaat cgtaaatgtc caggagcgac taaagaacca
     2941 gcttgatttg catgctcgcc aagtacttct ttcaatgcct tgtgcagtag atgggttgct
     3001 gtatggtttt tgatgatccg attgcgcatc gattgatcga tcaatagatg ataggtttgt
     3061 ccttcactca ttggtgcttt tacttctacg gtgtgcataa attgaccatt aggcgctttt
     3121 tgaacatctg taacaactgc tacgatttcg cctacttcat tcatgatcca tccttgatcc
     3181 gctacttgcc cacccatttc tgcatagaaa ggtgtttgat caaagatcat ttgagcttca
     3241 ccttctgcga cttcattcaa aagagactca cctttaacga tgaccaataa ttgactatct
     3301 gattcttgtt ctgtgtatcc aatgaattta ctttctactt tgatatctgt caataaagca
     3361 gattgtaccc ccattgattt ttcagtactg cgggcagagc gagcacgttc gcgttgtgca
     3421 tccatttctt tttcaaaacc agcatgatca actttcagtc cttcatcttc tgccacctct
     3481 tccgttaatt ctaccgggaa gccatatgtg tcgtatagtt tgaagatgtc ttttcctgct
     3541 agagtcgtcc cgttttcgtt ttttacttct tggatcactt gatttaagat ttccaaacct
     3601 tcattgattg tttcattgaa acgttcttct tctgtacgga taactttttc aataaattct
     3661 ttttgttgaa gtacttcagg ataataactt accatgattt ccccaacaac agggactaaa
     3721 cgatataaga atgcttgttc aatacctaat tttttcccat gcatgacagc acgacgaagc
     3781 aaacggcgta agacataccc tctcccttca ttcgatggca atgcaccgtc gccaatagca
     3841 aatgaaaggg cgcgaatatg atctgcgatt actttgtaag aaatatctgt tactgggttt
     3901 tcaccatatt ttacttgttt gcttagcttt tctacggcgt ggataatagg cataaacaag
     3961 tctgtctcaa agttcgttga cgcatcttga acgattgaga ccatgcgttc caatcccatc
     4021 cccgtatcaa tgtttttatg aggaagtggt tcaaatgtgt tatcaggctt atggttgaat
     4081 tctgaaaata ctaagttcca gatttctaaa taacgttcat tttctcctcc tggatagttt
     4141 tcaggatcgt cttctgctaa atcgttataa gcttctccac ggtcatagaa aatttccgta
     4201 tccggaccac atgggcctgc accgatatcc cagaagttgt cttctacatc aataatgtga
     4261 tctagtggca ggccaacttc ttcatgccag atgcgttttg cttctgtatc ttttggataa
     4321 actgttacat aaagtttttc tggatcgaat gccatccatt ttggatcagt caaaaattcc
     4381 catgcccagt ggatggcttc atttttgaaa tagtcaccaa tcgaaaaatt acccagcatc
     4441 tcaaacatcg tatggtgccg tgctgtttta ccgacatttt cgatatcatt tgttcgaata
     4501 gatttttggg catttgtgat tcttggattt tctggcacaa ctgaaccatc aaaatatttt
     4561 ttcaatgttg caacaccaga gttgatccac aataaggttg gatcgttcac ggggatcaaa
     4621 gacgcactag gttcaatcgt atgtccttta gattggaaga aatcaagaaa catttgtcga
     4681 acttgtgcac tacttaattg tttcatgtca ttacctgctt tctataaatt aaatacaaaa
     4741 aaacaccatc acagccaagg acgaaaactc gcggtaccac cttgattgca atgatgtgta
     4801 atcattacct cttaaactcg ataacgctga gctgcgtcgg tatttctacc aaaatagaaa
     4861 aagggagcaa tttgattagt gactattctt ttttcagcca agaaagaatt ttctgtgagt
     4921 cttaaaaaac aaatcatatc cttttcgtac ttaattagta tacgcattct attgatcact
     4981 gtcaatatct caatttttat tttcatttcc ttttggtcat actccacgat ttttggtacg
     5041 ataagaataa aggaatcaat acatctctgc tattcatgac aaggaggaat atgatgaaaa
     5101 aaatagttgg agcggatgaa tggatacgcg cagctagctg ctttttgaga tatcaagttt
     5161 ttgtattaga acaagggatt cctgcagaac tagaattcga cggaaaagat atcccgcaac
     5221 aaacctatta tctatttctt gatcaaaaaa gaccagtagc tactttgcgt tatgaaaaga
     5281 aaacacctac ttgcttcaag ccggaacgat tttgcgtagc aaaagaatac cgaggaaaag
     5341 ggttaggcgg tcagttgctt gaagctgctg aaaaacgggc ggtttcagaa ggttgtcaga
     5401 ctagttatgt agtagctgaa atgacggctg tagattttta tgaaaaacaa gggtatcata
     5461 cttgcacaga accttttatt gaagatgaag tactttgtat cggaatgaaa aaaagtctag
     5521 cttaaaaaca agagattgtg acacgccttt tgtcacaacc tcttgttttt tatttatgtg
     5581 aatttttctt atttttacga gcagtacggc tttgctgtct gcgttcgatt ttgcgttctt
     5641 ttttattttt ttctgcaatt gcccaatcga tttttttctt ataaccaggc ttgatctttt
     5701 tctttttctt cttcacaaga ccaatcagcg tgggatctaa tacatctttt gatttttctc
     5761 gtttggcacg tctattacga tcgtatgttt tgacgatttc cccgtttttg atttcttttg
     5821 gttcaaatga gacgcccaat ttttctatcg catcaatggc ttgctcatca tttggcgaat
     5881 ataacgtaat ggccgttcct tttaagccat tacgtccagt acgtccgaca cgatggataa
     5941 agaagtctag atcttctgga atttctgcat taataacatg agagacacct tcgatatcga
     6001 tccctctagc agctaaatca gtcgctacaa catattgata ctccagattc tgtacttgtc
     6061 gcatcactcg ttttctctct cttggggtga tgtctccatg aatctttgct actttcaagc
     6121 cttggttttt caagtaatcg gcaatttcat ccacatgctg ttttgtattg gcaaatacga
     6181 ttgctaaata aggatgacca attgtcagca gttgataaat catttgattc tcatctttac
     6241 cttttgttga aatcagccaa ttttcaatgt cttcagaaat aactgctttt ggtttgattt
     6301 cttcaatgat tggattttcc atgtattttc gtaagaatgg acgaagtttt tctggaatag
     6361 tggcagaaaa aactaaaaac tgcagctttt ctggtaatcg acttgcaatt tggtctactt
     6421 catttaaaaa acccatatcc aatgtcatat ccgcttcatc gacaacaaat gcaaatgcgg
     6481 tgtgaatctt cagcgcttgt tcattcatca tgtctaatat tctaccaggc gtgcctacta
     6541 caacctgtgg ctgttggtga tgcagacgat ttaattgacg ctgtttgtct gtaccgccga
     6601 caaaattcga gacacggatc tccggtgtag aaaatttagc gatttgtgta gctgcttcat
     6661 agatctgtgc agctagttcc ctacttggag ctgtaataac aacttgtact tcctcttttt
     6721 ctgcattgat tttttcaaac aaaggcaaca aaaacgtatg tgtttttcct gtaccagttt
     6781 gagattgccc aatgatattt tttccttttg caattaaagg aatcaaacgt tcctggacct
     6841 ctgtgggctg tttgaagccc tttgcggtta acgcttcatt gataaacggt ttgaagcgga
     6901 atatatcaaa attattcatt cgtgcacctc ttctctttaa attatcacga ctttggcatt
     6961 ataacacaga caaagcgttt ttgctcagct aatttcgaga atcatcatcc tttgtttggc
     7021 caatcgtgct aacagtatat gtgtgactag ctttttcata aaacagaatc cgtttttttc
     7081 tcaaaaatga tagcaagata tttaaaataa aaataccttg tagtaacaag acaccgagag
     7141 aaatgaatac tgcactcatc tgcaacaccg tattcgttgt cgaattcatg atttgctgaa
     7201 gcaactgccc caaccagctt aaaccataaa aaagaaagta cagatacccg tagcggatca
     7261 tcaatgcttt taaagaaatt cgttcgcggt cttcttccac caaacggata cgcacaattt
     7321 ttttgccaag tgtccatcca ttggttataa acggtacaat cataaagtat acccctactt
     7381 ctaataaaag ccaccagata tgagaagtaa ctgcccctga atcgaacgca attttaaaga
     7441 aaatcgccgc taccaaatta ataagagata aaaatgtcca gtcgatcaaa taagcgacca
     7501 agcgtcgagt caatgagaca gtctttcctt tttcataaga aagttcatcc attcgtcttc
     7561 ttgtaggaag cataaatgta aataacggcg taacgacaaa accaatgatt ccacccaatg
     7621 tatttgtcac taaatcattc acatcaaata agcggtatgg tcttgggtag ataaaataaa
     7681 gtccacttag ctgcgtcaat tcaaaaaata gagaaaggca gaaactagca atcatcgttt
     7741 tcataaaaga aagccggaaa taatatctta aataaatgcc aaacggtatg agtaataaaa
     7801 cattaaaagc tggttccaaa aatacactct gcttcattgc cggcaggtag gtactaggtt
     7861 cagacaagac caataccgtg ttgtctctga aatgttgcca tgaagcaaat aaatgcagct
     7921 ccatctttgg ccctgtcatt tgggcaaccg tctcctttgc cggaagtggt aagatcacca
     7981 aaaaatacgc acacaataaa taaaaaacga aagaatacaa aataactgcc cttgttacta
     8041 gaaatgttcc atactttcga tattcataaa taaaaaaaac agatgagatc gctaaggcta
     8101 aaaatggaaa aacaatcagc gcaaaacgaa tcggttcgat ataacttgcc atatgactcc
     8161 tccttgattc tttgtatctc tcttatctta gcagtccttt gctgtttcaa gcaagtaacg
     8221 aggctattat ttctcatttc tttcaaagta aagtaagata aacacaaagg gggtgtcgag
     8281 atgctgaata tgtactttgt ttttggcgta cctatttttt tactgttcct ttatgctacg
     8341 atcgcttatg taagaaaaag aactacaatt cattatttag gatttattct cctgattatt
     8401 tcgggtttca tgctagtctt caatcttcag acttggcagc aagcacttct tgaaatggat
     8461 aaaatgactc cacatgcttt gtctaaagtg cttggctacc ctgtctatct gatttggctt
     8521 cccattttca tttctggttg cttggtttta ttaaatattt atcgcggcgt acgccgaata
     8581 gttcagttac gcaaatcaaa gtaaaagaca gaaatcggtt gagcccgatt tctgtctttt
     8641 actttgctat ttcatcgtag acaaaacgac atttacgtga tcaaaattaa cgagtacttt
     8701 tgacatttct tccacgtact cctcatttaa ataaaatatt ttgtttggca gagaagtgca
     8761 gttctaataa aattcgaatg ttttagctat ctacgtttta aatagagata aatcgggata
     8821 ccagcaagtg tcaatactat ccccatcgaa gctaaaaagg tctgagtgat aagcgtgctt
     8881 gatacgataa aagtccctcc aatgattgct actagtggaa taaacggata cattggaact
     8941 ttgtatggtc gaaacaaatc aggttctttt ttacgaagaa gaatgacacc aacaaagacc
     9001 atcgtataaa aaatccaaat gacaaagatc agcatatccg ttagtgtatc aaaaccaccg
     9061 atcatcatca tgccaatagc gatgactagc tctaagatac ctgcggctac aggaacttta
     9121 gttttatcat gaagtttagc aaagagcttg ctaaaaggca ggttgttttc ttttgccata
     9181 acataaggga gacgcattcc agtaagggta tagccattga tggttccata aacagaaatc
     9241 aagataccaa ctgtgaccag tcttccaccg aagccgccaa aaatcatttt tgcaacgtca
     9301 cttgccgcat tgctgtttcc tgcgactccg tcgatagatg cggttcttaa gaacaccgca
     9361 tttactaata aataaacgat catgattccg atgattccta aagaaattgc ttttggcaaa
     9421 tctttcgccg gctttttcaa ttctcccgaa atatttccta cgtgtatcca accgtcgtat
     9481 gcgaacatcg ttgctagcaa gcccgctccc aaggctgaga aaaatgaaag attttctcct
     9541 gcttggatcg gaaacagttg gaagtctact ccttcttgtc ggaacaaacc gaaaatgacg
     9601 atcacaaaca aaggaataag cttgcagaca agcgtaatcg actgaaaagc ccctccagct
     9661 ttagaaccta aaaaattgat caacaggatc gatacagcag ctgtaacagc tacgggaaca
     9721 atcattgact gggaaagacc aaatagattg acgaactgtg taccgaaaat gatcgacaag
     9781 gctgctacat ttgctggaaa ataaatcacg acttgcgccc agcccaataa aaaagctgct
     9841 gtatttccgt aaatccgttc gatatattta atcatccctc ctgtttctgg aatagcagct
     9901 gccagttcag ctcctgttag tcctgcacag acagaaatca taccaccgag aaaccaagaa
     9961 aacatatgca aactggcgga tcctgtaact tctgctacgc ttgcggcttt aaaaaatacc
    10021 cctgcaccga tcactgtgcc cataacagtc gataaagcag taaaaaatcc catcgtacgt
    10081 tttaattcat tttctatttt tacttctttt actggcgtgg cttgatattc ttccatccct
    10141 tgtccccttt cataccatag ccaatcccaa aagtataaaa aactttttgg atgtggttct
    10201 tgttttatac cgtctctacc aatttttctt tcagtttttc caacatatcc tttgtcatca
    10261 catctaagtc gtatttcggc gaaaagcccc attcctcttg cgcacaagaa acatctagtg
    10321 aatttggcca agattctgct attgactgac gtacaggatc cacatcataa tccatttcaa
    10381 aatctggtat gattttttga atagatgctt tgatctcttc cgggtcaaaa gacatcgccg
    10441 atatattgaa ggcatttcga tgaaccaatc gatcaggatc tgcattcatg agttttataa
    10501 tggcatcgat cgcatctggc atatacatca tatccattga tgtaccttga tcaatgaaac
    10561 tagtgtactt tccttctttg attgctgaat aataaatatc gactgcgtag tctgtcgtac
    10621 cgcctcctgg caaggttgca taagaaatca atccagggaa acggacacca cgagtatcta
    10681 cgccatattt cacataataa taatcacata gtagttctcc agctaccttc gttaccccat
    10741 acatggttgt aggacgctga agagtatctt gtggggtatg atcttttgga gtatttggac
    10801 caaacgcgcc gattgaactt ggcgtaaaaa atttcagctg gaattctcta gcgacttcca
    10861 atgcattgac cagcccagtc acgttcaaat tccaggctaa ttgtggtttt gcttctgcaa
    10921 cagctgacaa aagagcagcc aaatgaatca atgtgtccac ttgatattgt tctgctatgc
    10981 tgcgcatttt ttcgtaatcc attacatcta ggatctcaaa gatatcgttt ttctcaatga
    11041 cttcaggcat gcgtatatct gtcgcaatga cattttcact gcctagttct tgacgtagcc
    11101 gtgtgatcaa ctctgaacca atttgtccta aacatcctgt caccaatact tttttcatga
    11161 tgatcctccc tgatcttttt tactactatc ttcagatgat taacaatata tgcgtgaaca
    11221 tgttctctaa ttttaaatag acgaaaaaac taaatttagt taaataattt ccatttcttt
    11281 tcctattttt tgataaactg ctactgcttc atcaagcatt tccctcgtat gttccgcagt
    11341 cggcatattt ctgatcctgc ctgtcccaag aggcacagtt ggataaacga ttggtttagc
    11401 ataaatccca tgttcgatca gcttcttaga gaaagtctga gtcacttttt cgtctcccaa
    11461 aatgactgga gtaataggtg tttcagacat accgatgtta tagcctactt ttttcaattc
    11521 ttctttaaaa taattagcat tttcccaaac tttttcgaca agttccggat gttcttgcat
    11581 caaagtaatc gaagccaatg cagctgcagc tgcacctgga gttagcgaag tagaaaatag
    11641 gaatggccga cttctagctt tcaaccagtc gattagtgtt ttcgatccag ctacataacc
    11701 cccaacaaca cctatagctt tcgataacgt gcccatttgc atatcgattt tgtcgcttaa
    11761 tccaaaatgc ttgacggttc ctgcaccttt tccagttacg cctgagccgt gcgcatcatc
    11821 gacgtaggtg atcagtccat atttttcaac aatcggaata atttccggca atcgtgcaat
    11881 gtctccatcc atggagaaaa caccatctgt gatatacatg atttttttat atttcttact
    11941 ttctatcgct tcctttgctt ttttctcaag gtctttcata tcttgatgtt taattcggat
    12001 gatttttgct ccggataagc ggcaaccgtc aataatagat gcatggttta attcatccga
    12061 taagatagca tcttcttttg tcattactgc agagatagcg cccatattgc agttgaatcc
    12121 agattgaaag gcgattgctg cttctgtccc tttgaattct gcgatttttt cttctaattg
    12181 ctgatggatt ttcagcgaac cattgatcgt tcggactgct cctgctccta caccataagt
    12241 ttcggttgct tcaatacatg cttttttcaa ttcttcgcga ttggcaaatc caagataatt
    12301 gttggatgct agattgatca tcttttttcc gtctacgatg atacaagcac cattttcact
    12361 ttctagtatg tcgatcgtat tgtataaccc cttttctttc agttgatcca attctttctc
    12421 taaatacatt gttagttctt tggaagacat tgtctccacc tccatattca tctgtcttgc
    12481 agaacatacc aaacatatat cgtgtcattc atctacaaag caagtatgct atatttttga
    12541 taacgcttac actgattatg agaattattt aaccacattt tttatttaca gtatcttttt
    12601 actttcaaat gacttatata gtatgaacaa ttacctttta gataatgatc tattttactc
    12661 tacctgggca gataaataca acacaaccat tgatagaagc cctttcgtta aaatcaattg
    12721 tattctgaat atttaattta tttattgcat agaccaataa aaatacataa aatttccatc
    12781 ttcgtttatc agcaattttt aattttactc tctgaatatt tcttaaaagt tttacttgtt
    12841 gaattcgaaa aaaggactgt gacaaaagtc ttgtcacagt cctattctt
//
LOCUS       DANKHL010000076        12838 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000076 DANKHL010000000
VERSION     DANKHL010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.079"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            128..1015
                     /locus_tag="PW101_001504"
     CDS             128..1015
                     /locus_tag="PW101_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDL2513908.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            1012..2181
                     /locus_tag="PW101_001505"
     CDS             1012..2181
                     /locus_tag="PW101_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513909.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            2205..3560
                     /locus_tag="PW101_001506"
     CDS             2205..3560
                     /locus_tag="PW101_001506"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDL2513910.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(3632..5437)
                     /gene="glmS"
                     /locus_tag="PW101_001507"
     CDS             complement(3632..5437)
                     /gene="glmS"
                     /locus_tag="PW101_001507"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDL2513911.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            5847..6137
                     /locus_tag="PW101_001508"
     CDS             5847..6137
                     /locus_tag="PW101_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513912.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            6150..7175
                     /gene="sppA"
                     /locus_tag="PW101_001509"
     CDS             6150..7175
                     /gene="sppA"
                     /locus_tag="PW101_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL2513913.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGASGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            7178..7837
                     /locus_tag="PW101_001510"
     CDS             7178..7837
                     /locus_tag="PW101_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL2513914.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            7994..8926
                     /locus_tag="PW101_001511"
     CDS             7994..8926
                     /locus_tag="PW101_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513915.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            9080..10369
                     /locus_tag="PW101_001512"
     CDS             9080..10369
                     /locus_tag="PW101_001512"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDL2513916.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            10616..11866
                     /gene="clpX"
                     /locus_tag="PW101_001513"
     CDS             10616..11866
                     /gene="clpX"
                     /locus_tag="PW101_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDL2513917.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            11949..12536
                     /locus_tag="PW101_001514"
     CDS             11949..12536
                     /locus_tag="PW101_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDL2513918.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(12610..12765)
                     /locus_tag="PW101_001515"
     CDS             complement(12610..12765)
                     /locus_tag="PW101_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513919.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
BASE COUNT         4215 a         2265 c         2791 g         3567 t
ORIGIN      
        1 aacgcatact tgcaattttc ctgaaaatta tgcgtttttc tcaagaaaaa tacgagcaga
       61 tcagcctcta aaattaaaac atatgatata ctagagagga aatctcaaaa gagaagagag
      121 gctttttatg tcatttcaaa tcggggaact ttttaatttg gactattggc agcaaatgtt
      181 ttccggtgac ttgttctctc tgaatttttt ggtgaacata cttgatattc tggttgtctg
      241 gtatcttgta tacaaactga tccaactgct gcgaggcaca aaagcgattc agcttctgaa
      301 aggtgtagct atttttattg ttatccgaat cgtagcggaa atcattggtc tgcacacctt
      361 gtcttggctg atgaaccaag tcattacgta cggggtgata gctgctatcg tgatttttca
      421 gccggaaatc cggagaggac tggaacatct tggacgcagt tcgcttttca aacaatcaaa
      481 aagcgaacag caggaagatg agaagatgat cttgtctttt gacaaagcca tccaatatat
      541 gtccaagcga aaaatcggtg cgctaattac tatcgaacgg catactggat tagatgagta
      601 tattgagaca ggaattgcat tagatgcaga tatcactgga gagctgctga tcaatatctt
      661 tattcctaat acaccgctgc atgacggtgc agttattgta aaagaaggaa agatcgcagt
      721 agcaagcgcc tatcttcctc tatcagaaag tatgttgatt ccaaaagaat tcgggactcg
      781 acaccgggca gctgtcggta taagcgaagt cagcgacgcg attaccatcg ttgtctctga
      841 agaaactggg gatgtcagca ttacattaga taatgaactg atggctggct tatcccagca
      901 agaatatttg gcaattttgc gtagagaact gattgctgaa gagaaaacaa aaaacaagaa
      961 aaacgtgatt caatctttcc tagacggtat gactaaggga ggggatcgca aatgatctca
     1021 aaagagaaac ggacaagcat tctttacagt tttattgcat tgctgttcag tattgcgctg
     1081 tacttcaatg caaatggcca gtccgttcca aatacgcttt ctggaaatga agcgtataat
     1141 caaacagtta ctggggtgcc aatcaagatt tcttatgatt caaagcgcta ctatatccat
     1201 ggatatgaaa atgcagtgac tgtcaaactt tccagtgcaa accgagttca gttgaatgca
     1261 gaggcaaacg aagatacacg gatgttccgc gtcacagcag atatcactaa gctgggcgaa
     1321 ggaacacatg aggttccgct aaaaattcaa aatttgagca gtgctgtcac agcaaagatc
     1381 gaaccatcga ctattacagt aacggttgag aaaaaggtaa taaaagattt cgatgtggaa
     1441 acgttgattc cttcaacgtt cacaccatct ggatatgaac ttgatgatac ttccgttagt
     1501 cctaaaaaag tctctatcac tacaggtgac aaaacattgg cagaaatcaa acgagtagta
     1561 gctgatgtgg acgcttcgat ggtcacagat gatgggatta atagtgaggt cccaatacag
     1621 gcgctgaatg cagcagggga agtattaagt atcgtctctg atccagtgca agtaacagtg
     1681 agagctgatt cgatcaagcc aagcaagtcg gtacgacttt atgggataca gcaaggaaca
     1741 ccagctgccg gggtagaaag ctatgatttc agtttttcaa aactggaagc agaagtaagc
     1801 ggctctagcg atttgcttgc atcgatcggt gacagtattg ctgtaccgat caatgtctca
     1861 ggtatcaatc atcgaacaac tcgtaaaatt gaaataccag tagaagaggg gttaagtatc
     1921 aagcccgaat cagttagtgt ggagattaca cccgtgttgc aaacgtcaag cagtagctct
     1981 gcttcgacga gcagtcggac acaagaaaat acaagtacga gtagaagtat ttcatctgct
     2041 gattcaggaa cggaaccatc gacaacaacc atttcttcca gttcggaatc tgggacaagc
     2101 agctcgacca caagtacaga agaaacatca gaaacaagcg aatcaacaca aatgagcaga
     2161 acaaattcct cagaaaatta agttaaagga gtaattttac aactatgggt aaatattttg
     2221 gaacagacgg cgtacgcggt gaagccaaca aggaacttac gccagaactt gcatttaagc
     2281 tgggacggtg cggaggttat gtcctaagcc agcatgaaga agaaggaaaa cgcccacgcg
     2341 tgttagtagg gcgggataca agaatctcag gtcaaatgtt agaagaagcg ctgatttcag
     2401 gacttttatc ggtgggtatc gaagtgttcc agctaggtgt gatctctaca ccaggtgttg
     2461 cttatctgac acgtttgcaa aaagcaagcg caggggtcat gatttctgct tctcataatc
     2521 cagcacaaga taatggaatc aaatttttcg gtgcagatgg ttttaaacta gtagatgacc
     2581 aagagctaga aattgaagca ttattagatg ctgaagtgga tgaattgcct cgtccatcag
     2641 ctgaaggatt gggaactgta gaagaatttc cagaaggctt attgaaatat tctcaattcc
     2701 ttgtacaaac gatcaacggt gacctttccg gtttgaccat ttgtgtcgat gcagcaaacg
     2761 gagcaacttc aacaagtgtt aatcgtttgt ttgccgatct tgaaacagat ttttatacca
     2821 tgggcacaaa tccagacggg ttaaatatca atgatggcgt aggctctacc catccagaac
     2881 gtttagctga gatggtcgtt gaaaaaggag cagatgctgg actagctttc gatggcgatg
     2941 gcgatcgtat tattgcggtg gatgaacttg gaaacattgt cgacggtgat aaaatcatgt
     3001 ttatctgtgc taaatacttg gcagaaaaaa atcgattgaa aaaagatacg atcgttacga
     3061 ctgtcatgag taacttagga ttccacaaag cggtggaagg aatcggtttg aaagatgtcg
     3121 ttacacaagt tggtgatcgt tacgttgtag aagaaatgcg caaaaacgat tataactttg
     3181 gcggcgagca gtctgggcac atgattttct tagattttaa cacaactgga gatgggatgc
     3241 tttcaggaat tcagttactg aatatcatga aacagacagg taagaaatta tcagagcttg
     3301 cagcagaggt aacaatctat ccgcaaaaat tagtgaatat ccgagtaagt gataaaaacg
     3361 gtgcgatgga tgttccggca atcaaaaaag tgattgaaga ggcagaagct gaaatgggat
     3421 cagaaggaag aatcctagtt cgtccttccg gtacagaacc attacttcga gtgatggcag
     3481 aagcgcctac tgatgagaaa gttaattact atgtcgataa aatcgcggca gtagtcaaag
     3541 aagaaatcgg actggattaa gagatatttt cataaaaaat aaaagaacaa ggaccagtgt
     3601 cagatgtctg acactggtcc ttgttctttt attattctac tgtcactgat ttcgctaaat
     3661 tacgtggttt gtcaacgtcg aatccccgta atagcgttgc gtaatatgca agtaattgag
     3721 tcggtacgac tgtggttaga gtagttagat aagggtggac agttggtagc accagctggt
     3781 cactttcctt ttctaatccc tctgagacaa taactagtgt tttagcgcca cggctttcga
     3841 cttctttcag gtttcctcga gtatgcgcac cagtcacttc ttcactgatg attccaatca
     3901 ctggtgttcc ttcttcgatc aaagcaatcg ttccatgttt caattcgcca gcggcaaaac
     3961 cttctgcctg gatataactg atttctttca atttcaaagc agcttcaaga gaaacgttgt
     4021 agtcagcgcc acgtccaatg taaaaggcat ttcttgtatc gctgagatat acttctgcca
     4081 actcttctaa ataatctttt tgggcgatga ccgattccat cgcattagca actaaactca
     4141 attcatgagt gatatcgaat acaagagaag tttcaaattc ttttttatca ccgatagctt
     4201 tggctaataa agttagtaca gctatttgtg cagtataagc tttcgttgat gctactgcga
     4261 tttcgggtcc agcatgcaat agcagtgtaa aggatgcttc ccgcgaaaga gtagaaccag
     4321 caacatttgt aatcgtaagt gaagggtagc cgagacggtt gatttttacc agaacttggc
     4381 ggctatctgc tgtttcgcca ctttgactca agaaaataaa gaatggtttt tttgaaagaa
     4441 gaggtgtgtt atagccaaat tcactggcta aatgaacctc aacagggatc tgtgtcaatt
     4501 tttccagagt ctgtttacct gctaaccccg catggtaact agttccgcaa gcaacgatat
     4561 agattcgatc gctatcagac aatgtatcga ccagcttttg atcaagagag acacgattgt
     4621 cttctgttac gtatttttga gcgatacgac gcatgataat aggctgttcg tcgatttctt
     4681 tcagcatata aaaaggatag gtacctttct cgatatctga agaatctact tgtgcttcga
     4741 atgaagaacg ttctacgatg tcacctgcaa aattttgaat aataactttt tctgatgtga
     4801 cggtaaccat ctcttcatct tcaatctcaa cgaattcctt cgtatgagca agcatagcca
     4861 ttgcatcgct ggcaatcaca ttgaatccat cgccgagtcc gattaatagt ggacttttat
     4921 ttttagcgac gtaaataaca tcaggtgctg tccgatcaat caaagcgaaa gcgtaagaac
     4981 ctttgatgac ccgcaaagct tttaaaaacg cctcttgcgt tgtagtatcc ttcgcgaaag
     5041 tttcaattag atgagcgata atctcagtat cggtctcacc aataaagtgg tcatttgcta
     5101 aataagcaat tttaagttct tcaaagtttt cgattacgcc attgtgaacg agcacgaagc
     5161 gtccactttg ggaagtatgt ggatgggcgt tttcttcaga aggttgcccg tgagttgccc
     5221 agcgtgtatg accaatacct atatttcctg tagtatcaat atttattttg tttttcaaat
     5281 tttgaattct gccttgtgct ttgaccagat aatcagttgt gccatctgag acaaagatac
     5341 cagctgaatc ataaccacgg tattctaatt tttctagacc gtcaatcaat cctggtgtta
     5401 cattttttaa tccaatcatt ccaacaattc cgcacataaa aaacatccta ctttctataa
     5461 tgaattcgta tgaatgcttc cagctgcagg aaaatccttt tgttttatct ttgcagatat
     5521 tttttgtgga ttttctgtag agtgcagtcc ctagggccat ccgccgaatc tttcgataaa
     5581 ccctctcctc gtcatctgtt tacacagacc tggcgctatc ctaaaaggta taactatcct
     5641 cctcttttgt tttttatctg gaaacattta aaatcataac cttttgcgaa tgttcttgtc
     5701 aacaaaaaaa gaataaaaaa taaattggta tatatcaaat gctttgtcaa atgctctttt
     5761 tatgtcaaaa aataatgggt ttttatctat catctttttc aggagttcgg tacaatagaa
     5821 agtaatcgga tagaggagga gtaaaaatgg attcaaaaat tgagatcatg acactgggaa
     5881 tgttaaaaaa acaattgtcc aaattcgaag catcagctgg tgtatcagat gatacgaaaa
     5941 tatttttaga tactggctgg gatagcatcc aagaaatcgc acctgatgcc ttggaagttg
     6001 ctgaagctcg tgagtttacg gtagaagatg aattgacaaa agagtcgttt tccgggtatg
     6061 cgcgggaaga aaaagcagag cggttcgaca cttctgagca gtcagaaact gtgatcgtca
     6121 taaaaaactt gtattagaaa ggagaaagaa tgaataagag acgttgggca gcagttctga
     6181 ttgctgcagg attatttgtg gtttcgttag tttcagctag tttgactaag cctcaggaaa
     6241 acgatcaaat tgagggattg aacagttggt tatatgggga tgaagaacta gcttcaaatg
     6301 tgttggaaga gggagacagt acgcaaaaaa tcgtaaaatt atcagtagat ggaacgattg
     6361 ctgcgggagg ggcttcaggt ttatttgctt ctgaaggtta caatcataaa aattttatgg
     6421 aacaactgaa ggcgattgaa gaagatccaa cagtcaaagg tatcttttta gaagtaaatt
     6481 ctcctggtgg cggtgtctac gaaagtgcgg aaatcgctaa gaaattggat acgattcgta
     6541 aagaacatga tatcccaatg tatgtcagca tgaaaaacat ggcagctagc ggtggctact
     6601 atatttctgc tcaagcagat aaaatctttg caacagaaga aacagtgacc ggctcaattg
     6661 gtgtgatcat gtcaggattg aattattcag gacttctaga aaaactgggt gtggaagata
     6721 caacagttaa aagcggagcg ttgaaagata tgggttcagg aacgcgacca gagacaaaag
     6781 aagaagaagc ggtgcttcaa gcatatatcg acagcgcata tagtcgtttt gtagcaatcg
     6841 tcagcgaggg acggaataaa tctgaagaag aagtcaaaaa aattgctgat ggaaggattt
     6901 atgatggtgt ccaagcaaaa gaagtaggtt tagtcgatga attgggattt cctgaagatg
     6961 ctttaaaagc tatgcgaaag gaacaacagc ttgaagatgc ggaactagta gaatattctt
     7021 ccacgccatc tgggtttgct agtacatggt taggaagtaa actagcagaa ttccagggat
     7081 tgaagccttc tgagactagt cagatcttat ccgttcttga gaatctaggg acagcggaat
     7141 caccaaaagc aatgtattat tacggaggtg agtaagcatg tcagagcttt caaatgaaaa
     7201 taaggaacaa gaaaaagata agcaacagca gcctgctgta ggacctgact ccaacttttc
     7261 ccaaaaggta ctgagctctt ttgaaagaga gcctttgacg cccgaagaaa tcaaaaagaa
     7321 acaacgtaaa tggaaaaagt atacggacac aagaaatgaa ccgccaaaag caaatgattt
     7381 tcctaagtat ttctatgcag gtttttttat ccgaatgttt gctttcttgg tcgatctttt
     7441 gtgtatcgga gctattacgc gtttaacact tggtattgcc aaaaatctag gttggcttaa
     7501 tttttcagat tcttatctga gtatctacgg attcacagcc ttacttattt atctagcgta
     7561 ttttatttta ttgacgaaat tcaatcatgg tcaaacgatt gggaaaatga tctttggtat
     7621 tcgagtcatc agctttgatg aagaagaact tagctggtca accgttttag ttagagaagg
     7681 tgcttgccgg ttcattctaa aatttccttt gctgatgtta gggtatcttc cagcaatctt
     7741 taatcagaaa aagcaacatc taggcgattt tttcactaat acaagtgttg ttacgatcaa
     7801 tctgatcaaa gcattcaacc aggaagtcaa cagctgatga gcaataaaaa gcagtaacag
     7861 ttgaaagcac cttctaaaag tcagatctaa aatctagctt ttagagggtg cttcagttat
     7921 ttttaattgt catgcttttt ttgttttgct actattgaac atgagctgaa catgagcaag
     7981 aggaggacac tgcgtgaagc cgattgattt tccagaaaaa tatgagaatt taatgcgagt
     8041 agcacaacaa gcactagcaa accagcaata tcagcaggca aaagaattat ttcaacgagc
     8101 ttacgagttg aaagaaagct ttgaggctaa tagcctgctt gttttttgtc tttacgagtt
     8161 ggacgagaaa aaagaagcat tgaagcaagc actcttgcat gaaaaacagt atcttcaaaa
     8221 tgaggagttt gctgaatttt attttgactt gttaatcagt acacaagatt ttttatatgc
     8281 tagaaaattg atagcaagta cagattttta tgaagctttc gagcagcggg tcattgaaaa
     8341 aatccaattt gcagaagaat tatccggaca aatggagagg cagaagatca aggcactcca
     8401 caataaatcg gaggaacttc cttcgttgga acctgcacgt cagcttgcgt ctattgatga
     8461 gatcgaacag cttccttatc atgagtttat ccaaacagcg agtaaactta ttgtatcgcc
     8521 agaggtccat attttagcaa gggcaaaatt actggagaca cttaggcaat tgaacgaatg
     8581 ccatcctgtt ttctatttga cgattgaaga aaagttagtc aaagtcatac caaaagatct
     8641 gccaaagccg cagcagcagg caagttacca gcaattatgt gtattttcag atcgttacgg
     8701 taatgaagat gcgctgctta gttccgtgtt aaatgaagaa ttcacgttgc aatctgcaat
     8761 tgtttatcct gtctatgata catacatcga agacccgaag cgctggtttc aactgactgt
     8821 agaggcgtgt acaggaaaaa cgatgtatga aggtacagaa gatgaaaaac aagatttttt
     8881 aaaaaaaaga gaaaaaattt tgcagcaaat gatcttcttc cattagattt tatcagtgaa
     8941 aaagataaaa atgttatgaa aaacaaggat tctatccatt caatatgttt acaatttgaa
     9001 agggttagtg tactatttaa aggtatgcat gaaacgtttt tgatgtataa aatgaatttc
     9061 taaactcgga gggaattaaa tgactgcaac atttgaaaaa aaaggcacga atgatggtgt
     9121 tttaaaattt acgattgcgc aagacgaaat ccaaaaagga ttaacaactg cgtttaataa
     9181 agtgaaaaaa agcttgaacg tacctggatt ccgtaaaggg aaagtttctc gtcaagtatt
     9241 caaccgtatg tacggtgaag aagctttata tgaagatgca ttgaatgctg tattaccaga
     9301 agcttatgaa gctgctgtca aagaagctgg catcgatcct gtttctcaac caaaaatcga
     9361 tgttgaaagc atggaaaaag gtcaagactg ggtaatcact gctgaagtaa cagtgaaacc
     9421 agaagtaaaa ttaggacaat ataaagatct agttgttgaa aaacaagacc gtgaagtaac
     9481 agatgctgac gtggatgcac gtatcaaacg tgaacaagaa gcacaagctg aattagttat
     9541 caaagaagat gctgcagctg aaaatggcga tacagtagta atcgacttcg aaggctttgt
     9601 agatggcgaa gcgtttgatg gcggaaaagg cgaaaattac tcacttgaat taggttcagg
     9661 ctcattcatt ccaggttttg aagatcaatt agttggtcat aaatcaggtg aaagtctaga
     9721 tgtaaatgta actttcccag aagattatca agcagaagat cttgctggaa aagaagcagt
     9781 cttcaaagtg acaatccatg aagtaaaagc aaaagaagtg cctgaattag atgacgaatt
     9841 cgcaaaagat gttgatgacg aagtatcaac attagacgaa ttgaaagaaa aatatcgcaa
     9901 agaattagta gaaacaaaag aaaaagcagc ggacgatgca aaagatgaag cagcgatccg
     9961 tatggctgtt gaaaacgcag aaatcgttga attgccacac gtgatggttc atgatgaagt
    10021 acatcgttca atggacgaat tcttgaacaa catgcaacgt caaggtatct ctcctgaaat
    10081 gtactatcaa ttaactggtt ctacagaaga agacttgcat aaacaattcg aaggtgaagc
    10141 agaaatgcgc acaagaacaa atcttgtgat cgaagcaatt gcagctgctg aaaatcttca
    10201 agcaacagaa gaagaaatca atgcagaaat ccaagaatta tcagaaacat acaacatgcc
    10261 aatcgaacaa atcaaacgcg tgttgacaga agatatgctg gaacatgatg tgacaatgaa
    10321 aaaagcaatc gaattgatca caggtacagc gacagaagcg aaagaataga aatcaagtaa
    10381 taaatcattt ctgaagaagc tacttctaga aaattgttta ttcgaaataa gggactgtga
    10441 cacgattttg tcacagttcc ttattttttg ttaagagtaa aaaatgcaaa ggtataggtc
    10501 agttgatcct ttgttaaaca ctttttcttt ttactaaact atgttatgat agtaaaagac
    10561 tgtaaagtct ttgatagttt cttgcatact ttttgcaagt taaggggtga agaccatgta
    10621 tgacaatcca agtagcaatg agaccgtacg ctgttctttt tgcgggaaaa cccaagaaga
    10681 agtgaaaaag atcgtagctg gtccaggggt ctacatctgt aatgaatgta tcgacctatg
    10741 taaagaaatc attgatgaag aattttatga agaagctgta cgcgaattca ctgaagtccc
    10801 taaaccacta gaaattcttg aagtattgaa taactacgtg attggacaag atcgcgcaaa
    10861 acgctcgtta gctgttgccg tatacaatca ctataaacgt gtcaatcagc aagcacaaga
    10921 aaacacagat gatgttgaac tgcaaaaaag caatatctgc ttgatcggac ctacagggtc
    10981 tggtaaaaca tttttagcac aaacattagc aaaaacattg aatgtacctt ttgcaatcgc
    11041 agatgcgaca agtttgacag aagctggtta tgtgggggaa gatgtagaaa acattttgct
    11101 taaattgctt caatctgctg attacaatgt ggaacgtgca gaaaaaggga tcatctatat
    11161 tgatgagatc gataaaattg cgcgtaaaag cgaaaatgta tctattactc gtgacgtttc
    11221 cggcgaaggt gtccaacaag ccttgctgaa aattctagaa ggaactgttg caagcgtgcc
    11281 acctcaggga ggacgtaaac atccacatca agaatttatt cagattgata cgacgaacat
    11341 attgttcatc gtaggtggtg catttgatgg catcgaaacg attgtgaaga atcgtatggg
    11401 cgaaaagacg attggatttg gtacacaaaa ccaaaaactt tctgaagacg aaagtgtcat
    11461 gcagcatatc attcctgaag atttactaaa attcggattg atccctgagt ttatcggacg
    11521 attacctgtt atggcagctt tagagaaatt gacaacagat gatctggtac ggatcttgac
    11581 tgagccgaaa aatgcgctag tcaaacaata tcaaaaactt ctttctctag ataataccga
    11641 acttgaattc gaacctgaag cgttgagagc cattgcgaaa aaagcaatcg aacgcaatac
    11701 cggtgctcga ggacttcgtt ccatcattga agaaatcatg atggatgtga tgtttgatat
    11761 cccttcaaat gaaaacatcg aaaaagtgat tatcacaaaa gaagcagctg aattgtctgg
    11821 tgagccaaca gtaatctata aagattcaga gaaaaaagcg ggttgatgaa aagaggctgc
    11881 cgtaaaagtc attttggctt ttgcgacagc cgcttgcttt attcatcacc ttgttaagga
    11941 gagagaccat gaaagtccat caagctgaaa ttatcataag tgcagtggca ccaaaacaat
    12001 atccagatac acaattgcca gaaatagctc tggcaggacg atcaaatgta gggaaatctt
    12061 cttttatcaa cacattgatt gatcgaaaga atttagcccg tacttccggt aagccaggaa
    12121 agacccagac attgaatttt tatttgatcg agaacgcatt acattttgtc gatgttccgg
    12181 gatatgggta tgcaaaagta tccaaaactg agcgagcaaa atggggaaag atgatcgaga
    12241 catatttaac gactagagac cagctacgtg cggtcgtttc tttagtagat ttgcgtcata
    12301 agccttctca agaagatatc cagatgtatc aatttttgaa gtattatgat atccctgtaa
    12361 ttgttgttgc cactaaggca gataagatcc ctcgtggtaa atggaacaag catgagagcg
    12421 aaatcaaaaa agcgttggat tttgatcgca atgacacgtt tattctcttt tcttccgtta
    12481 caaaacaggg aaaagaagaa gcgtgggcag caatcgaaca agcaattgaa tcataaaaag
    12541 aataaaaaag acttaccagt caaactcttt cataaaagtt tggctggtaa gtcttttttt
    12601 gagcttaact tacttttgtt tcttgtcttt tttcttttca ttttgtggct gtggttttag
    12661 aaatcgatcg atctcttctt ttggtgcaac tgttttttcc ggatcgacag caaatgattc
    12721 gaaaagttta tctaaattat ctgtacgttc aaatttcaac cccattgtaa acacctcttt
    12781 gtttcttttt acttagtcta tcatgtaatc gtccaaattg gtttctaaga cataaagt
//
LOCUS       DANKHL010000077        12738 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000077 DANKHL010000000
VERSION     DANKHL010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.080"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            97..540
                     /locus_tag="PW101_001516"
     CDS             97..540
                     /locus_tag="PW101_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2513920.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(546..1730)
                     /locus_tag="PW101_001517"
     CDS             complement(546..1730)
                     /locus_tag="PW101_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HDL2513921.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            1998..2696
                     /locus_tag="PW101_001518"
     CDS             1998..2696
                     /locus_tag="PW101_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL2513922.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2703..3380
                     /gene="nth"
                     /locus_tag="PW101_001519"
     CDS             2703..3380
                     /gene="nth"
                     /locus_tag="PW101_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDL2513923.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3506..4039)
                     /locus_tag="PW101_001520"
     CDS             complement(3506..4039)
                     /locus_tag="PW101_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513924.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4336..6717)
                     /locus_tag="PW101_001521"
     CDS             complement(4336..6717)
                     /locus_tag="PW101_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL2513925.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6707..7354)
                     /gene="recU"
                     /locus_tag="PW101_001522"
     CDS             complement(6707..7354)
                     /gene="recU"
                     /locus_tag="PW101_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDL2513926.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7418..7957
                     /locus_tag="PW101_001523"
     CDS             7418..7957
                     /locus_tag="PW101_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDL2513927.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8047..8466
                     /gene="gpsB"
                     /locus_tag="PW101_001524"
     CDS             8047..8466
                     /gene="gpsB"
                     /locus_tag="PW101_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDL2513928.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8488..8855
                     /gene="rnpB"
                     /locus_tag="PW101_001525"
     ncRNA           8488..8855
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PW101_001525"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9064..10230
                     /locus_tag="PW101_001526"
     CDS             9064..10230
                     /locus_tag="PW101_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDL2513929.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10276..11772
                     /locus_tag="PW101_001527"
     CDS             10276..11772
                     /locus_tag="PW101_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HDL2513930.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11853..12536
                     /locus_tag="PW101_001528"
     CDS             11853..12536
                     /locus_tag="PW101_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2513931.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         4273 a         2230 c         2630 g         3605 t
ORIGIN      
        1 tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa taggaaacaa
       61 aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca actgctaata
      121 gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga aatggaaaaa
      181 gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg gaaaatccct
      241 gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc catcagtaag
      301 ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga agagaataaa
      361 aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag acacgagcaa
      421 ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc aaaagaagaa
      481 tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag tagcaaatga
      541 agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa tccgaatacc
      601 gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc gatgacgcgc
      661 gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt atctgagaca
      721 gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa accataacag
      781 ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt gatgaataga
      841 taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga aaattttttg
      901 agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat caaagagaag
      961 cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg agaaaagata
     1021 gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa aggaataacg
     1081 gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga gaaacttaaa
     1141 tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc ataaacggca
     1201 tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa ctcagctaat
     1261 agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc catccatcca
     1321 aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata taaggtggag
     1381 atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca tatcgaattt
     1441 cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat aaggatcgga
     1501 tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa ggcagctaga
     1561 gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga gtgagaataa
     1621 ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc agtgaataag
     1681 acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat gtaaaaactc
     1741 ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt gtttccttgt
     1801 caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag tgaagatacg
     1861 catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg accgatcagt
     1921 gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt atactgtgat
     1981 tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga caaacagtca
     2041 tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa gaatttttat
     2101 ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat ttgcagatga
     2161 ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat cgtctcgtaa
     2221 caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca gatcgatata
     2281 atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa aaaaaatacg
     2341 aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa caagaatttg
     2401 gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa gaagaccatt
     2461 atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag gcttatagtt
     2521 ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt acaaaacaac
     2581 aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa gtagaacagt
     2641 cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag gagtagcgga
     2701 atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga atgtttcccg
     2761 aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca gtgatcttaa
     2821 gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt gcctcttttc
     2881 cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa ataaaaacga
     2941 ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag ctgattgaac
     3001 gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct ggtgtaggca
     3061 gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc gcagtagata
     3121 cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct acggtcatgg
     3181 aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag acacatcata
     3241 cactgatttt ttttggtaga tatcattgca cggcaagaaa tcccaagtgc gaggtatgtc
     3301 ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa aaggcgctaa
     3361 aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta tgaattatct
     3421 tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt aaatgtacat
     3481 actcagtagc taggatggta acttattaca atttattgcg ataaaaataa aagttgttat
     3541 ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct ataaaataag
     3601 tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt gtaataaaaa
     3661 tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg gatttgggcg
     3721 gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa aaagtgagaa
     3781 tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt aaaagtacag
     3841 aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat aataaacaaa
     3901 atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa aaaagatggt
     3961 gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag ccttctttca
     4021 aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg ggattattaa
     4081 gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata aacaagtaaa
     4141 tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta agctaaatgt
     4201 tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac agcttggtac
     4261 atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac gcttgtagac
     4321 gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg gtgtatggtc
     4381 atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag gagcagatga
     4441 agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag acgctggctc
     4501 gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc tttgactagt
     4561 agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct ggacatagct
     4621 ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat attgatcttt
     4681 gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt ctgtatttgt
     4741 cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta cgtgtgacga
     4801 ttctgaagtg acaggcgtca tttttttatt gtaacctgtc cacacggaaa tcgaataatt
     4861 tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac caagttttgc
     4921 atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata acccagcgat
     4981 ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg tgatcatata
     5041 agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt ctgttccgtc
     5101 ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat tggcgaaagc
     5161 cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat cttgttcttc
     5221 cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc caagattttt
     5281 taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg ctggaacatt
     5341 acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat tatcccagtt
     5401 tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag tttttccagt
     5461 agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca tcgtagaacc
     5521 aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt ctgcgatgtg
     5581 acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg tggaggcaac
     5641 ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat catatagttt
     5701 cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt agacattctt
     5761 atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga cgatcttagt
     5821 attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat taacagcttg
     5881 atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa cagtatctcg
     5941 tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg cttgaggcat
     6001 cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac caaagtacat
     6061 ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat ttacgtagta
     6121 ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc acgcttcttg
     6181 ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa gtttgatcaa
     6241 ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag agagggcaga
     6301 accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat cttctaccga
     6361 cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt cacgcttttc
     6421 tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag atacggtagc
     6481 gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga aaagtcctac
     6541 acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct tgataaataa
     6601 tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct tcttcacact
     6661 ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat ttgccattct
     6721 tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc aaataaggaa
     6781 tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa gataaaggta
     6841 gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga acactattca
     6901 aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc ccattttgtt
     6961 cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag ggaaaagatg
     7021 ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag acgccattat
     7081 agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca cttctcttag
     7141 gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc gccaacccgc
     7201 gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc ccccgattgc
     7261 cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag gcattcttat
     7321 aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct aggccacatt
     7381 atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa aagtaatcta
     7441 tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc ctaaaatcca
     7501 agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag ggctggaatg
     7561 ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag tagcggaatt
     7621 gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg attttggtaa
     7681 taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag cagattatgt
     7741 cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc acactcgttt
     7801 tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc ctggaaaaac
     7861 gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg aaattcggct
     7921 gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt tgatttatca
     7981 acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa aactagaggt
     8041 gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga attcaaaaca
     8101 aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat catcaaagat
     8161 tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg attgagtgca
     8221 aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca acaaacagag
     8281 gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc aaatcttgaa
     8341 cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc agcacaacct
     8401 aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa aacacgtcaa
     8461 ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga cttgatcagg
     8521 ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc ttagcgaaac
     8581 cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct tcggctatgc
     8641 ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat ccggtggaac
     8701 gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt gtctggaaga
     8761 agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg ttttttctcc
     8821 ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag ggaattaggt
     8881 cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta tttttcaata
     8941 cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta ttcggaattt
     9001 gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat aaggagattg
     9061 tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg gctagaagca
     9121 ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa cggacgtgca
     9181 cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg tacagcagat
     9241 cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt gtttgaaaac
     9301 gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc tgtagctgga
     9361 aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac aaaaaaagca
     9421 atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact gacggaaact
     9481 ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt aactctggat
     9541 acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg agcaccattg
     9601 aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga ccgtccattt
     9661 tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat aggccataat
     9721 atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt cagtcaagaa
     9781 atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat tgaattagat
     9841 attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa tgcagaagaa
     9901 atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt caaaacggat
     9961 aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg agaagaagaa
    10021 actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa aacatggagt
    10081 aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa agcaaccaaa
    10141 aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg gggaactcgt
    10201 ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc aattggatgt
    10261 ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga aatcaatctt
    10321 ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc tgaaaaagct
    10381 agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc aaaaaaaatc
    10441 ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga attgtcggaa
    10501 gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca tcgcgtaccg
    10561 gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca atggcaaaaa
    10621 gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa tattgaatta
    10681 acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga tgttttgctg
    10741 aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga tcagcttaaa
    10801 gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc tgaaacagac
    10861 tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa agtaatcgaa
    10921 caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc atttatgact
    10981 ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga tttcaaaatg
    11041 gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga catcgatgat
    11101 aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga atcacaatcg
    11161 ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca atatcctttc
    11221 tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt ttatcgttca
    11281 ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac ttaccctctc
    11341 catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt agatgttgct
    11401 gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg accaacaaat
    11461 gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg ttattttccc
    11521 tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca gcaagaaata
    11581 gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg gatgtcagag
    11641 catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga tgcaacacat
    11701 gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt taatgtatat
    11761 caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct atagatgttt
    11821 tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg atcgctgatt
    11881 atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat accaaaagag
    11941 ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc tttgtgacgc
    12001 ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat gagaacgagg
    12061 caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa cagatctcga
    12121 tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa gcggccgaac
    12181 tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta ggagcaatcg
    12241 gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt gatcctgaac
    12301 tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca gtgatcaatc
    12361 atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac ggatatgaag
    12421 aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt ctggaatggt
    12481 ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa aaatgatgga
    12541 ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta aaagttactt
    12601 ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt cacaaggaac
    12661 aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt cggaattggc
    12721 aggagaacac cgtttatt
//
LOCUS       DANKHL010000078        12734 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000078 DANKHL010000000
VERSION     DANKHL010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.081"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="PW101_001529"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="PW101_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL2513932.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="PW101_001530"
     CDS             complement(323..892)
                     /locus_tag="PW101_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDL2513933.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="PW101_001531"
     CDS             1078..1980
                     /locus_tag="PW101_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2513934.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="PW101_001532"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="PW101_001532"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL2513935.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="PW101_001533"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="PW101_001533"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDL2513936.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="PW101_001534"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="PW101_001534"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDL2513937.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="PW101_001535"
     CDS             8548..8733
                     /locus_tag="PW101_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDL2513938.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="PW101_001536"
     CDS             complement(8795..9991)
                     /locus_tag="PW101_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2513939.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..11597)
                     /locus_tag="PW101_001537"
     CDS             complement(10161..11597)
                     /locus_tag="PW101_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL2513940.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11890..12699
                     /locus_tag="PW101_001538"
     CDS             11890..12699
                     /locus_tag="PW101_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2513941.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3801 a         2673 c         2250 g         4010 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtccaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa
    11581 ccgaaaaact tttccataaa aagttccact ccctgaattt ttttctgatg gaaaactata
    11641 ataataaaaa ataagtaaaa tagggcaaat ccatcgaaca atttcaaatt atatagacgt
    11701 ttcattcgct tttcaacgtt tttttaacaa aaaaagcgaa aataatactt aacgttcgtg
    11761 tttagcttat gtttagctat ttatatagcc ataactgtag tttttgttag gaatagctga
    11821 ttgctcttgt taattaccta aaatatgcta gactaaaaca aatgctagtt gaggaaggaa
    11881 ggacccttat tgaataaaaa attaattgcc atcgatctgg acggaactac cctcaatcaa
    11941 gattcgatga ttacatctaa aacaaaagag actctaaaaa aagcaatcaa tgccggtcat
    12001 catgtcagta ttgctacagg acgcccattc cgaatgagcc atcagtttta ccaacaatta
    12061 gaattaacaa caccgatggt taattttaac ggcgcattag tacacatccc taatcagcac
    12121 tgggatggtg aaagagaaac gttgatcaat cgtgaaatcg cttttgaaat cttatcgcaa
    12181 aaaaagcagc taaatttaga ttttatcgct gctgaaaatc gtgatacttt ttttattgac
    12241 agttttgatt tctttgatga aaaaattttt gcttcttctc gtccaggaga gaagaatttg
    12301 ctttctccta aaaatttgac gacaaatcct acatctttgc ttgtccgaac agacaaacgt
    12361 tttgcaggag ctgtatctgc tgaattaaca cgtcaatttg gcagttatgt cgatgttcgg
    12421 acatggggtg gacctactgc catcttagag attgtttcaa aaggaatcca aaaagcaaaa
    12481 ggcgtgcaag agattgctaa ctacctgtct atcgatcaaa aagacgtcat tgcctttggt
    12541 gacgagcata atgatttgga attgttggat tatgcaggtt ggggagtagc tatggctaac
    12601 ggtactgacc aactcaaagg cattgcaaat gatgtcactc ctttgagcaa tcaggaagac
    12661 ggtttagctg tttatctgga aaaactactt aaactataat agagaattag attccacaaa
    12721 aaagaggttg tgac
//
LOCUS       DANKHL010000079        12696 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000079 DANKHL010000000
VERSION     DANKHL010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.082"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            269..1192
                     /locus_tag="PW101_001539"
     CDS             269..1192
                     /locus_tag="PW101_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2513942.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            1401..1979
                     /gene="pgsA"
                     /locus_tag="PW101_001540"
     CDS             1401..1979
                     /gene="pgsA"
                     /locus_tag="PW101_001540"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDL2513943.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(2369..3448)
                     /locus_tag="PW101_001541"
     CDS             complement(2369..3448)
                     /locus_tag="PW101_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2513944.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            complement(3563..4387)
                     /locus_tag="PW101_001542"
     CDS             complement(3563..4387)
                     /locus_tag="PW101_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL2513945.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(4360..5040)
                     /locus_tag="PW101_001543"
     CDS             complement(4360..5040)
                     /locus_tag="PW101_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL2513946.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(5063..6001)
                     /locus_tag="PW101_001544"
     CDS             complement(5063..6001)
                     /locus_tag="PW101_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDL2513947.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(6246..7109)
                     /locus_tag="PW101_001545"
     CDS             complement(6246..7109)
                     /locus_tag="PW101_001545"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDL2513948.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(7251..9824)
                     /gene="mprF"
                     /locus_tag="PW101_001546"
     CDS             complement(7251..9824)
                     /gene="mprF"
                     /locus_tag="PW101_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL2513949.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            9965..10771
                     /gene="proC"
                     /locus_tag="PW101_001547"
     CDS             9965..10771
                     /gene="proC"
                     /locus_tag="PW101_001547"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDL2513950.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(10850..12406)
                     /gene="rny"
                     /locus_tag="PW101_001548"
     CDS             complement(10850..12406)
                     /gene="rny"
                     /locus_tag="PW101_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDL2513951.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT         3643 a         2791 c         2426 g         3836 t
ORIGIN      
        1 acggaaaaat atctccttat attttgccgg tttttgagct aataccgagg aacagctgat
       61 tgaacaccgt ttaagaaaga ggtcgtgagc ctgccgttta actccgagtc acaaggaaca
      121 agttcaaaaa acagcttctt atatttattg gatattgtga cttgtgaccg aggagttggc
      181 aggagaacaa tgtttattcg gaaaaaagga gctgtgacat gacttttgtc acagcaccca
      241 aacataacgt ctttcgttgc aacagcagat gcttcggaga atcaaacaga ctggagaaga
      301 tgaacgagtg gcttttgaaa ccatcggaga aaaattacgg caagcacgcc tgaataaaaa
      361 aatatcttta gatgaattac agcagattac aaaaattcaa aaacgttatt tggaagcaat
      421 cgacaatgat gattttgacc aattgcctgg taaattttac gtgcgggcat ttattcggca
      481 atacgctgaa gctgtaggag aagatggaga ccatttagtc gatgtttttg atgggaaaag
      541 caaattgaca ggatcggtcc ctgaacgtcc gcagcctgaa acagtaagta cttctagaaa
      601 agcaatgcat caggaagaat cgcatccaag tcgcttttgg acaagtcttc ctgtcatttt
      661 attaggattg gttgctttgg cgattgtcgt tgtcgtgggc tatatgactt ggcaagatcg
      721 tcaagctgat ccaataatcg gagacacagc ttcttcgctt aaagtagaag gatcagtcga
      781 ggagtcctct tcctcagagc aaagcacgca gaccaaagag tcttctacag tagaatcaac
      841 aacaaaatca agtgaagaaa agaaaatggc aatagctaca ggagaagatt ctggcagtgc
      901 catcgcagta aatatcacag acgctaccaa gcctgtaacc cttgaattta ctgctgtcaa
      961 tcgtgtatgg ttaggtgtgc tagtagacaa tgcctatgtt tatcaaggga cactggcagc
     1021 aaatgaaaca cagagtaccg tgttaccgga aacagcaaca aatgcgacca tcacgattgg
     1081 tgcagcaagc aatgcaacga tcaaagcgaa tggagaatcc gtaccagtta atccagggga
     1141 aaacaatcag agtccaaaaa atgtgaacct tactcttcaa tacgcagaat gatcgtataa
     1201 attagtttac gcatctgttt acttgtaggt ataaaaaaga aggatcgtga caaatctgtg
     1261 tcgcggtcct tcttcgttaa ttcaaaaaga tcaatttcaa taaaaatact cctttgaata
     1321 aattcatttg gaaaagtaaa taatatcggt ggaaacttcg ctgatagtca cgtagaatag
     1381 ggaagaaagg agtcgatagt gtgaatttac caaataaatt aactgtactt agaatttgta
     1441 tgatcccgat tttcattttg gttgtaggtt tacctttaga ttggggacaa tttaacgtag
     1501 gagacaccac actggctgtc acacagctag taggtgcagt tatttttgct ttagctagta
     1561 tcacagactg gttagatgga aaaattgccc gtgcgcgagg ccttgtcact aattttggca
     1621 agtttgcgga cccgttggca gataaaatgc tagttatgac tgcttttatc atgttggttg
     1681 accaaggaaa ggcccctggt tgggtagtag cgatcattgt ctgtcgtgaa ttagccgtca
     1741 caggcttgcg tttactactt gtcgaacatg gagaagtcat ggctgcagct tggccgggaa
     1801 aagtcaagac ggctacacaa atggttgcta ttatcttgct gtttatcaat aatatcccat
     1861 tcagcctgat cggtattcca gtagatcaaa tcatgcttta tgcttgtctg atctttacta
     1921 tctattcagg tgtggattac ttcatcaaaa acagcggtgt gttcaaagga tcaatgtaag
     1981 tatcgtttag tggagaggtt gtgagcctgc cgtctaactc tgagtcacaa gggataattt
     2041 caaaaaacag cttttcatgt tttatgtata ttgtgactta tgaccgaggt tttggcagga
     2101 gaacactgtt tatttggaac aaggagctgt gacaagattt ttgtcacagc tccttgccct
     2161 ctttttaaat aaaaaaatcg taaatttcaa gtttaagtta gccacccatg gtgacccggt
     2221 tgctcgttac caccaaccgt ctaatttttc taatcgagtg ggcccttgac aatgagctga
     2281 ggcatggtag tctcccgact ggctggcccc ggccagcttt ccagacacaa gccatgccga
     2341 gggaggctcg ttgtcaaggg cccacgtgtc aagccgtcaa ccgttcctgc ctaaatgcct
     2401 ggacgaacgt gtcatgcggc gttgcatagc ccaaaatctt tcgcgggtaa tcgttcatcc
     2461 aacgctgaat ccgcaagatt tggttttcgc tcacgttgcc gattgggctg cccttgggga
     2521 tgaaacggcg aatcagcttg tgttggtttt cgctcgtccc gcgctcccag gaagcatagg
     2581 ggtggctgaa gtagatgttt gccacatcct gcaaggcatc gtgcagtccc gcgaactccg
     2641 agccgttgtc cgaggtaacc gtcttgaaca gctggccgaa gtacgcacca gcccgttctc
     2701 tcagctgtcg gaccgcttgg ttgaccgatt cggcatcctt cccggccagc ttcagaatga
     2761 cctcgaagcg tgtctgccgt tccaccaacg tcagcagcac ggcatccgac ttggtcttgt
     2821 ttcctacgac cgtgtcgact tcccaatggc cgaaggtttt ccgggtacca accgcctccg
     2881 ggcgctcttc aatggatttc cctagcaccc gtttattcgg gcggttgcca tgacgggacg
     2941 atttcggttt gcgggacagt ttttctagca ggtccatatt cttcgttttc atgatgccac
     3001 ggtcgatcca ctgatagagg gtggtggtcc cagggataat ggccgggtcg aacaattcgt
     3061 gttttctggc aaagcccacc accacgtccg gggaccattt ttccaacagc atcatgtcgt
     3121 ccgcccattc aatgaaggcg tccgtgtcgg cccataaggg ccgtctgccg cagttcaagc
     3181 ggttcttctc gtaggccgcc tgcccggcat cggcgtcgta gacgtcatga taatagtcgt
     3241 agaccttccc gttctgattc tggcggttaa gctgagtaac ggtcccgcgt ttgatttcgt
     3301 tgtttatggt ctgcggcacg cagcccagga catccgcaac ctgacggttg gaatagtttt
     3361 cctttttcag gatggcgatt tggcaacgtt ctgagtaggt taggtgtttt cccttgcgtg
     3421 aaactgtgtt atcatggttt tgcgtcatgt gaattcatcc ttgtctattg agagttgtgg
     3481 taacttcaat ataacatgga attcacgtgg cgttttttta tttccaaatg gctaagttaa
     3541 ttataaaatc cgcctttata atttagttat tttttcttct gaaggcaacc aatattcgtt
     3601 tgatgatatt tacaatcagg aacaaagcaa taaagactaa tgtgattgtt gctcctggag
     3661 gtgtatctag ataaaacgag ctagttagtc ctgacagcat ccctagaaat cctacacaaa
     3721 cactgatagc aatcacgaca ttgaagcttt tgcctaatcg cataccgatt gctgcgggaa
     3781 gaaccatgat tgcggatatc aaaagtgcac cggcaatcgg gatcatcaca gcaatagcca
     3841 caccagtaat cacattgaac atcatcgaca tcaaatgaac gggtagccca tccacgtggg
     3901 cggtatcttc atcaaaagtc agaacataca tcggccgttt gaataaaaag aacaatatac
     3961 cgatggcaac gaataagatt cctaaaaaga tgacttgctc ccatgtaatg gtcacgatcg
     4021 aaccaaacag ataggattgg atacttgttg ctgaattccc tccgctcaaa ttcatcaata
     4081 ctaacgctag cgccaaacca ccagccatta aaatggcaat ggagatttct gaataggttc
     4141 ggtagatcgt tcgcagatac tccaatatca cagctgctaa tacaacgact agcatggtcg
     4201 taagtgtagg attaaggttc aaaaagaatc ctaacgctac ccctgctaaa gagacatgag
     4261 aaagcgtatc agccatcagc gattgtcttc tgatcactaa aaacacacct agcatcgggg
     4321 cgattccggc aatgaagata gcggcaagaa acgctcttct catgaattca tatgaaagca
     4381 acgccattga gaatcctcct ttctcaccag tcggatctgt ctgtccgcat actgtttgat
     4441 atcttcatga tcatgggtga tcattagaac tgatttccca tggacatgcg cactatgtcg
     4501 gagaagacga taaaattcat ttctggattc ttcatccata cctgttgttg gttcatcaag
     4561 aacaaataag tcagggtcag tagcaaaaat acgcgctaaa ctgattcgct gcttttgtcc
     4621 gccagacaat tcacctattc gccgatttcg catatcccac atccctacag cttctaaagc
     4681 acgctgtaca tgttgatgat ctttttcaga gagacgcttg aaccagcggc ctcttggata
     4741 tcttcctgaa cggactaatt cgatcaccgt actaggaaaa cctgcattga aagatgcaac
     4801 ttgttgagga atataaccga tacttaattt ttttccttct tgatttttct cagcaatctt
     4861 tatcattcct tttgaaggtt tcagtaatcc taacgatgct ttcaccaagg ttgacttggc
     4921 tgctccattt tctccagtta aaatgacaaa ttcacccgca tctacatgat aagagacatc
     4981 ttctagtaca ggttcatcat catagtaaaa ggtcaaatca gatatttcga tataacgcac
     5041 cttacaacct ccattctaaa acttaattga tactttcttt taaagctttc agattttttt
     5101 ccatgacaga aatataattt tcgcctgctt tgatttgttc attagtcaag ctttctaaag
     5161 gattcaacac ttctagcttc actcctgttt cattcgacaa tgtctcagct accttcgaag
     5221 aggcattttc ttcaaaataa atcactttga tgttgttttt cttcacaaat tcttttagct
     5281 cagctaagcg acttggtgtg ggttcctgat ctggtgaaac gcccgaaatc gctacttgtt
     5341 ttaagtcgta ttgattcgct aaataagcaa aagcagcatg ctgagtgaca aatgtgcggt
     5401 ttgttgcatc ttttagctca tcggagtatt tttgatccag tgcttcaagc tttttgatat
     5461 aagcatcggc attcttcgta aaagtttcct ttttatctgg atatttttcg cctaattctt
     5521 cagcaatcgt tctgacttct tttatagcta ataccggatc gagccaaaca tgtggatcgt
     5581 attcatgtga atgaccatgc ccttcttctg attcttcatg ttcgtctgct tcttgcgatt
     5641 ctaaacggtc gattccttta gctgcttcga tcatcaaggt ctgtttgcta tccactgaat
     5701 cctttaacga atccacaaaa aattccatgt ctgaactatt atacacgaaa acatctgcat
     5761 cggaaatttc tgctctttct tttgcacttg gttcgaaatc atgcggctcc gttccagcag
     5821 ggatcaaaag tttcacgttt ccttcatccc ctacgatctc ttttgtaaaa tcatacatcg
     5881 gataaaaagt cgtaacgatc gtcaaatcct cttttttgtc tgcttccttg ttcgtatttc
     5941 cacatgctgc aaacagtaaa gcacctatca ttaccgtagc tgtcaaaaga tattttttca
     6001 tttatatgct cctttcaatg aacaaatcgg aattttacga tttattaacg tgaactaatg
     6061 tatcaaaata aaaaaacaca gtcaatagaa atccatcaat ttctgattta agcatgtata
     6121 ttcttgatta aatcgtaaaa tggatgagtt gctgctcata tctagccatt tcactacaat
     6181 aaaaaactga agaataccat tttaggattc ctcagttttt catcacctat tttacttctt
     6241 atattttatt gcagggttcg tactaaataa acttcctcac aaaaaccttt taacccatta
     6301 tatactctct gcgctcttga ataatgttga cacaaggcaa aaacggttgg tccgcttcca
     6361 ctcatcaatg cagcatctgc tccgtacttg atcatacgct ctttgatttg atggatgact
     6421 ggatgccgct ttcccgttat atcttctaaa ctgtttccca tttttttgat catctgctga
     6481 taatcttgct ctcggatggc ttcagcaagt ccgtggatgt cttgatgttg gatggcttgc
     6541 gtatctactt ctctaaacac tgtacgtgta gagacactca ttcttggttt gaccaacaca
     6601 acccagcact gtggcataga aggtaatact tggatttctt cccctttgcc accgacaaat
     6661 gccgtacttc cgtagacaca gtaaggaaca tctgtaccga cttccaaacc gatttctgcc
     6721 agttcttcta acgaacagcc cagattccat aattggttga tccctcttag agcagctgca
     6781 cagtctgtac ttcctccgcc taatccagct gctactggaa tattcttctt aacaaagatc
     6841 ttgacaccgg ttttgatatt gtatttattt tttagcaata aagcagcttg ataaacattg
     6901 ttccgttcat ctaccggtaa aaatgcttta tttgtttcta caatgatttt gtcttctgtt
     6961 atttcttcaa aaaatagatg atctgccaag tctacgctcg acatcaccat ttccaactca
     7021 tgatacccat cttgccgtct atataataca tccagtccta aatttatctt ggctggcgct
     7081 ttttcaatca attccatcct ctcacccatt cgaaaagtct aagtcgcaag tattctatca
     7141 tagggaaaaa taaaagtata gcttatgtct gcctagtggt tgattttgtc atcgtttcaa
     7201 cagctttcac agaattttct cttcataatc caaacaagtt ctagtgattc ttataattct
     7261 gtgagcactt cttctgccaa actcggaaca tttccttttt cttttcctat cagactgagt
     7321 agctgcatgg tagcaaatcc gaaattactt cctttcctat aagcaaaata acgtccttcc
     7381 caatgatcga catattttaa tttcttttca taagcagact ggaaggaata gaaaaaatta
     7441 ccataattat atacgatatg catgactttt tcttgaaaga aagaaaaatc tgtttctcca
     7501 acattagcta atggagctaa gcctagattg gcttccgagt agcccatttt ctcgtataca
     7561 ataaacagat tggctaacag atagtcagct aattcctcag attctttagg taagacccgc
     7621 aacaaatcat atcccgcctg cttcttcgtc atcggctgag cggtgatgaa gccaacgact
     7681 ttttgatctt tacggacaat accgacaccg ctagataata catatgattt atcaaatcgt
     7741 cccacagaga agtatttttc tcgtgcacct tgaagccatt tttgtgaaac ttcatttaca
     7801 gcttgatgta attcttccgg cagttgatcg taccaagtaa aatggtatcc ttctttcatc
     7861 aaacgttgat attcaagttg ctgactcagc cgagacaagt cttctgttgc tagatcaaca
     7921 atccctgctt cacctacttt cgtgaagtga aaacccaatt catgaagcat cactacgtag
     7981 ctttctgata tcttataaaa cgcaagctga tagcctaaac aatctgcttg tttcataaac
     8041 tgattcgttg ctgcccgcat cattttagga ttgccaactg gatctcctaa aacaaagcat
     8101 ttgtttcctt tgatctgata accaaaaacg acttgatctt ggtcgtcttc ttggtaataa
     8161 aaataactgt aacctgacaa gcgaagctga tggcttgtag ctgtgcctcc gtatgtttcc
     8221 aataaacggt caaaacggtc tttttcgtac tcactgccaa gcttcgtagt atcttcagct
     8281 aaatattgat aaagcgccac taaagctagc aatgagatgc ccaaaccaat aagccctgag
     8341 aaccacacat catctgaagg aaacaaaata aaatgatttc ctgttattcg accgactaaa
     8401 aagcgtccgc tgtgatagcc tgcaattgcg taaacgataa acaaaaagcc gaacaagcta
     8461 gcatcaaaga ttatagctcc ccaagaataa acgaattttg ttcgataaaa ttcttttctg
     8521 gataaccaca cagctaacag aatcaagaca tataccaata tcaactgcca agataacgta
     8581 cgtgtgattg tatttaaaat accaaaagat agtaaagcaa tcgtaggcag aaatgccttt
     8641 ttgactttta ctgaaagtgc tctagcaagg ccgagtaata aaaagccaac caatagattc
     8701 actgtctgat ctaaaaaatt gaatgagaac ggcaataata cttgaaatag ccgactgatt
     8761 gcagacagat tagtgattgt tgatagaagt accatcatga tccccgcaaa atagacagca
     8821 cttacgagca cgatatgcgc aaccttttgg aaaaacagtc gcggaatgtt atctaaaaat
     8881 cgattgattt tgactcctgt atgtgtgatg aacaatagaa gtcccgtcat aaaaggtaga
     8941 acataataaa acaaccgata atataatagc caaactactg ctacatcttg ccgaacaggc
     9001 aattgactca ttcctaagat catcaaaacg tcaaaagtcc ccattccacc tgggaccatc
     9061 gtcagcattc ctatcaatgt agcaatcatg aacataggat agatcgaaag catcgaaata
     9121 tctattctca tcaacttgcc gatgatcaaa aataccgcaa gggctcccgt ccattgtcca
     9181 aaggatgcca gtagcagacc aagcaagcct tttggataaa actcgaagaa caatgtcttt
     9241 ctctttagat aagcaaaaaa gaaaatgact ggtgctaaaa aactaccgcc gaaaagccag
     9301 atccaatagc ttcgaaaaat actttctgac tgaataaaaa agacatcaat aaatgtaaga
     9361 aacgaccata cagacaaacc agtcagcata aatagtgcga cttttgatac agtggctaaa
     9421 accatttttc tatttgtctg ctttccataa aaactagccc gcagacttgc tcctaccaca
     9481 ccaccaaaac cggcgagatt gttgatcgtg tttgttaccc acgcagcaag aagaaactct
     9541 tttctttcca tttttggttt tccttgtttt tctaagactc tgatcgtcac ccaatcatac
     9601 aacaacatcg gagtgacacc cagcagtcca gccagaatca tgagcaggat cgtcatgcgg
     9661 ctttgttgtc ccatcctttg ccaaatatct tgccaactca tgccattagc aatatttgcg
     9721 acttgatttg ccacaaagac taacacggag ccgaaaaaaa tcagttttaa aatcagacta
     9781 tgttccttta tccattgcag catcaagtat cgtttatttt tcacattttc ccattcctaa
     9841 cttgtatact tttgtgtcat accccaatgt tatcttattt attttaattt ttccacttta
     9901 gacctgttct ttgcttgcga tgatagaaaa agaaagatat cctaatgcta aaggagagga
     9961 taacatgaaa gtaggattta tcggcacagg gaatatggca caagctatta ttgatggttt
    10021 attaaaaaaa ggaacagtct cgaaagaaga ggtgttcgtt tccagtggac attttgaaaa
    10081 tgcacaaaaa tttgctgctg aaactggcgt ccatgcatgc aaaagtaatc aggaagttgc
    10141 atcacaatca gatatcgttc ttttagccgt gaaaccaaaa atcattgaaa aagtattgga
    10201 agaactaaga gaatatagcc agaaagtctt attcatttct attgcttcag gaaaatcatt
    10261 aacagattta gaaacagcgc ttgaagatcc tcaggcctcc atcgtacgtg tgatgccgaa
    10321 tgtcaatgtc agcgtaggag caggcgtttc tgcgatttgc aaaaatgaag ctgccacgga
    10381 ggaacagctt cagcaagcta cgatgatttt tgaagcaatc ggcagcgtct atcctttagc
    10441 agaatcagat ttcagtacat tcattggcct tgctggcagc tcacccgcat tcgtttacat
    10501 gctgatcgat gcgatgggac ggactggtgt actgaatggt ttgtccaaac agacagccac
    10561 tgagattgct gcaaaagcaa ttatgggttc ttgtcaaaaa ttcctttcaa gtgataaaaa
    10621 tccatgggaa cttgtagacc aagtttcttc tcctggcggt acaactgttg caggtgtagt
    10681 tgccttagaa gaagcaggat tcatcccagc aatcatcaaa ggaatcaata cgacgatcga
    10741 gaaagatctt gagatgttga ataaaaaata atgatagcaa tgttttttcg tgccttacaa
    10801 aaaaagaaga gctgtggctt ttgccgcggc tcttcttttg tattttttat tatttagcat
    10861 aatcagttgc acgagtttcc cgaataacga ttactttgat gtgtccagga taatccagtt
    10921 cttcttcgat tcgtttacga atatcacgta ctaaacgaac agcgtctaaa tccgagattt
    10981 cttctggctt gaccattacc cgaacttcgc gtccagcttg aactgcgtaa cttgattcta
    11041 caccggcgaa gctgttcgaa atattttcca gacttcttaa acgacggatg tagttttcta
    11101 atgattcact tcgcgcacca ggtcttgctg ctgagagcgc atctgctgca gctacaagga
    11161 cagagataac agaagtagct tccacatctc catgatgaga agcgatcgcg ttgattacta
    11221 ctgggttttc tttgtacttg gcagctaatt ccgcaccgat ctcaacgtga gatccttcaa
    11281 tctcatggtc aagtgctttc ccaatatcat ggagcaaccc ggcacgtttt gctaattgga
    11341 tatcttcgcc aagctctgct gctaagatac cagctaattt tgcaacctcg accgaatgct
    11401 tcaagacgtt ttgtccataa ctagttctga atcgcagacg tccgatgatc ttgattagat
    11461 ctggatgaag tgtatgagca ccgacttcaa acgctgcctc ttccccgtat tcgcgaatac
    11521 gttcatccat ttctttacga gatttctcga ccatttcttc gatacgcgct ggatggattc
    11581 gtccatcttg tatcagtttt tccaaggtca tccgtgcgat ttctcttcgg attggatcga
    11641 atccggaaag aacaactgct tccggtgtat catcgatgat taaatcgata ccagtcagcg
    11701 tctcaagcgt tcgaatatta cgaccttctc gtccaatgat acgacctttc atttcatcat
    11761 tcggaagggt aacgactgaa acagtcgttt ctgaaacttg atctgctgcg cagcgttgaa
    11821 tcgctaatga taataagttt ttcgctttgc gatctgcttc ttctttggca cgctgttcag
    11881 attcttttac cattagagtt aactcatgat tcagctcttc ttcagtcgat ttcatgatga
    11941 catctcgtgc gtcctctttt gataaaccag caattttttc tagttctttt tgttgatctt
    12001 caatcatttt ttcaacgtct ttttctcgct cttcaattaa ttgctgtttt gaactaagtg
    12061 cttcttcttt actttcaagt gagttctcgc gtttttctaa cgagtcatct ttacgattca
    12121 gcgtttgttc gcgttgaatc aaacgattct cttgagattt taattctaac ttactttctc
    12181 tcagctcact ttcaacttca gaacggtatt tctgattttc ttccttagct tctaacaatg
    12241 cttctttttt tagagtttct gcttcttttt ttgcactatc gatgattcca gcagctgatg
    12301 attgtgcacc agctatttct ttctcgtgac gtgactttgc gaccataaac cctaaaccaa
    12361 gaccgacaat taaaccgatg atagcgagga gaatgttgaa taccataaaa tccacctcca
    12421 tactatcttt ttcattttta tcatctatac agcttctatg ataaattatt ctaaactcgt
    12481 caatatgcat cttcgcatat atgtgtacaa aaaacatgca caactttatt ctaatgtttg
    12541 taccccgtag tgtcaacttt aaataaacaa agaaacggct ttctacttat tcattaaacg
    12601 atggttttta aaataaatga cactagtttc atataggtaa acctttaaat tgagaaactt
    12661 ttctattaaa ttattctttg tagatgaaca gatatt
//
LOCUS       DANKHL010000080        12680 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000080 DANKHL010000000
VERSION     DANKHL010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.083"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(284..646)
                     /locus_tag="PW101_001549"
     CDS             complement(284..646)
                     /locus_tag="PW101_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513952.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(720..1100)
                     /locus_tag="PW101_001550"
     CDS             complement(720..1100)
                     /locus_tag="PW101_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDL2513953.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1164..2357
                     /locus_tag="PW101_001551"
     CDS             1164..2357
                     /locus_tag="PW101_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2513954.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2434..3018)
                     /locus_tag="PW101_001552"
     CDS             complement(2434..3018)
                     /locus_tag="PW101_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2513955.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3219..4217
                     /locus_tag="PW101_001553"
     CDS             3219..4217
                     /locus_tag="PW101_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2513956.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4329..4838
                     /locus_tag="PW101_001554"
     CDS             4329..4838
                     /locus_tag="PW101_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDL2513957.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4868..5527)
                     /locus_tag="PW101_001555"
     CDS             complement(4868..5527)
                     /locus_tag="PW101_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDL2513958.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5529..6293)
                     /locus_tag="PW101_001556"
     CDS             complement(5529..6293)
                     /locus_tag="PW101_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDL2513959.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6301..6978)
                     /locus_tag="PW101_001557"
     CDS             complement(6301..6978)
                     /locus_tag="PW101_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDL2513960.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7015..8400)
                     /locus_tag="PW101_001558"
     CDS             complement(7015..8400)
                     /locus_tag="PW101_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2513961.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8610..8694)
                     /gene="ffs"
                     /locus_tag="PW101_001559"
     ncRNA           complement(8610..8694)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PW101_001559"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8717..9232)
                     /gene="tadA"
                     /locus_tag="PW101_001560"
     CDS             complement(8717..9232)
                     /gene="tadA"
                     /locus_tag="PW101_001560"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDL2513962.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9339..10196
                     /locus_tag="PW101_001561"
     CDS             9339..10196
                     /locus_tag="PW101_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2513963.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10277..10612)
                     /locus_tag="PW101_001562"
     CDS             complement(10277..10612)
                     /locus_tag="PW101_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513964.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10679..12049)
                     /locus_tag="PW101_001563"
     CDS             complement(10679..12049)
                     /locus_tag="PW101_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDL2513965.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12127..12663)
                     /locus_tag="PW101_001564"
     CDS             complement(12127..12663)
                     /locus_tag="PW101_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2513966.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3768 a         2573 c         2118 g         4221 t
ORIGIN      
        1 tttaaatttt ttagaaatgt gatataacat ccacttcaga atcaacgctg ttaaatttat
       61 catgtatttc ttccttggtc aactataaat taagcattac tttgtgaaat gatttttcga
      121 tcaattatta ttattcgtta tacaaccgtt ttcaacatat tttgaaaata aaatgcacta
      181 aaaaactatc tttagcagaa catttcattg tattttttgg accaaacaat aaaaaaagaa
      241 gtttgaaaaa aattcaaact tctttgttcg cttaatctgt attttagaag aaaggcactc
      301 cctctacttc gtaagttcca agaacagagc gcatttgacc tggttcttcc caaatatatc
      361 cttcgcctaa ttcttctctt tgattctttt gttcagtttc aaagacaaac aacggaacat
      421 tttcctcttt aatatgtgta tttgtcaatt ccatcaaact aatcttgcta acatcgatat
      481 gaactatttc ttttaagaaa ttaagtatat tcgctaaacc agtctttcct tcagaaagtt
      541 ccgctaacgc aaattccaac ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat
      601 cctccaaatg caacataatt gtaccagcaa ctcttttgct tgccaaaatt tccacctcga
      661 tcaacttgtt ctatggctta cataaaaatt ctagcacatt ttcagaaaaa atacactttt
      721 tatttcttat tttctttccg ttctagttca gataatgctt cttctatcca aataggtaac
      781 tgctccgcaa tagtggtaaa tccgatctta cgatttttat tcgtgaaata cctttttctg
      841 cgatatggaa cagctggatt tgttttttct aacgttctta aagataaact gatttttttg
      901 gaatattcat caatgtcgat gatctgaact gttactggct gtccgacttt taacaatgaa
      961 tggatatttt tcgtataacc ggattgtact tctgaaacat gaataagtcc ttgcgtctcg
     1021 ttatcaagag aaacgaatgc gccataaggc tgtatccctg tcacttttcc ttcaattatt
     1081 tgtccaattt tataagtcat ctacttccct ctcttcgcat ttatcattat actgtaaata
     1141 gctcaaaaaa agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa tttgataaac
     1201 agatttcaag aaaagataca gaaagtgtaa aatgggacgc aattgctgag acatatcaaa
     1261 tggatgactt acttcctttg tgggtagcag atatggactt cttgtctccc ggtggggtag
     1321 ctaaagcgtt tagtgaatac atcaaacatg gaatctttgg ttatgccact ctgtctgata
     1381 aactatatca ggctatcatt cattgggaac gccaacacca tgctgtggag ttgacgaaag
     1441 aaaatatcgt ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta caaactttta
     1501 caaaaccagg cgattctatt ttgattcatg acccagttta tcctccattt tcctctatta
     1561 tagaaacaaa tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat aatcatttca
     1621 tcatggactt gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt
     1681 tatgcaatcc tcacaatcct ggagggcgtg tttggagcaa agaagaattg aaacagttaa
     1741 gcgaattgtg tttaaaacat caagtgattc tgtttagtga cgagatccat caagatttaa
     1801 ctttattcga tcatacattt acttccatgc taacgattga tcctaattta gatgatctac
     1861 tagttgcttt tacttccgca accaagactt tcaatctagc tgcaatcaaa aattcaatgg
     1921 tatttatcaa aaatccagag atgaaagctg attttgaaaa acatttagtg atgaaccaac
     1981 agcacgaaat caacactttt ggactgattg gaacccaagc ggcttatgag acgggtggag
     2041 aatggttaag ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt
     2101 ttgaacagta tcttccgaaa gtgagaataa tgaaccctga aggaacttac ttaatgtggt
     2161 tagattttac cgcctatatt tcagacgacc aagaattgga gaacactctt gttcggaaag
     2221 gaaaagtcgt attaaatcct ggcatcacgt ttggtccttc cggtcatggg catatgcgct
     2281 tgaatattgc ttgtccagaa gaaactttac tggaaggact taatcgaatc aagtatgctt
     2341 tagagacaat cgattaaatc atttattcaa aataaaagga cgagacctta ctaggtttcg
     2401 tcctttttca taattactcc gtaccatacc aaactattct gaaatttcaa ttgtttcaat
     2461 cactacatca tgtacaggtc ggtcttgtgg accacgttgt acatttgcga tttcatctac
     2521 tgtgtccatc ccttctagta catgtccaaa gactgtatgt cggaaatcta gccaaggagt
     2581 tccgccacct ttatacgctt caatgatttc tgatgggaat ccagcacctt ctagttgtcc
     2641 aagcatatta gctggcacat ttgtgttatt tacaataaag aattggcttc cgtttgtgtt
     2701 tggacctgaa tttgccatag ataaagctcc gcgaaggttg aacagttcac gactgaattc
     2761 atcttcaaaa ctttcgccat aaatactttc gccacccatt cctgttccag ttggatcgcc
     2821 gccttggatc atgaaatcag gaataacgcg gtgaaaaatc acgccatcat aataaccttt
     2881 tttggaaagt tcaatgaaat tttgaaccgt cttcggtgct agttcaggga ataattgaac
     2941 agtaatgtca ccacggtttg ttttgataac agcttttggt cctttttcac tttttaaatc
     3001 tagttgtggg aatgccatat tgtttcctcc taatatttgc atacttcata ataataataa
     3061 cataatacct aacctctcgt aaaaatacta gatttgtttt cttttatttt tcatcactag
     3121 atacattcta taacttaccg tttcgatttc gaattcaaac atttttgaga agcattctca
     3181 attaaattct gatatagtgt gaaaagggag gaatttttat ggaaatctat gacattacaa
     3241 tcatcggtgc aggtcctgta gggatgtttg ccgcttttta cgcaggaatg cgccaagcta
     3301 aaacaaaaat cattgacagt ctacctcaac ttggtggtca attagcaact ttgtatcccg
     3361 aaaaatatat ttacgatatt cctgggtatc cagcaataaa agcaagtgaa ttaatcgatc
     3421 aattagaaaa acaattaact acttttaacc atacattcca tttaaaagaa gaagttctct
     3481 cgcttactcg tgaagacgaa gttatcgaga ttactacaaa taaaggcatc cattattcca
     3541 aagcagtcat tttagcttta ggaaatggtt cattccagcc aagaaaattg aatttagata
     3601 atgctgaatc atttgaaaac cacggattgg attatttcgt gaatgatttg atgaagtatg
     3661 ctggaaaaaa agtcgctatt gctggaggtg gtgattctgc tatcgattgg acgttgatgt
     3721 tagagcctat tgcaagcgaa gtttacttga ttcatcgtcg cccggaattt cgtgcgcacg
     3781 aacatagtgt atcacgcttg aaatcttcat ctgtcaactt gcttactccc tatctcatcg
     3841 atggattatc aggcaataat ggggaactga cagatatccg gttaaaaaaa gtgaaaagtg
     3901 acgagacgat tgatttgatg attgattctc tgatcgtcaa ttatggattc tcttctaatt
     3961 tagaacatct ttcttcttgg ggacttgata gtaccagaaa tgccattact gtaaaatcag
     4021 atatgtctac ttctattcca ggcgtttatg cagctggtga catctgtacc tatgaaggaa
     4081 aagtgaaact gattgctact gggctcggtg aagcacctac tgcagtgaac aacgctttac
     4141 actatattaa tccaaaggaa cgaactcagc ctggtcacag cacaagctta tacgataaaa
     4201 atcttcgtcc tagttgattt tttatatctc tttgcatttc aagttaaacc cgaaagcagt
     4261 ttcttgcatt tttttcatgt tccgttaaca tgaaatagag aaagaaaaaa taaaggggga
     4321 gcaataatat ggtcgtcaaa actgagactt tagaagaaaa ggcaagagaa ctattgaagg
     4381 aacgtggcgt aactgtagaa gatatcgcac aacttgtcat gtttttacaa aaagactata
     4441 tttctgattt gactttggcg gattgtactg aaagtgtcaa cagcgtgcta acaaagcgtg
     4501 aagtacataa cgccattatc actggaatcc aattagatat attagcagaa aagaaaaaat
     4561 tgatgagtcc gttgcaacat attatcgaag atgacgaagg cctttacggt atcgatgaga
     4621 tcctagcctt gtcaatcgtg aatgtttacg gtacgatcgg ctttacgaat tatggttata
     4681 tcgacaaggt gaaacctggt attttaaaag aactaaacag ccacgatggc gttcatgtcc
     4741 ataccttctt agatgatatc gtcggagcga tcgccgctgc tgctgccagt cgattagcac
     4801 acaaagaacc agaaaaaaga agtcgcctta ctcaataatt caacaaaaaa agaaagaaaa
     4861 cgtctattta gacgagttct ttcttttttg tttgtttcaa gctatttttt cctaagaaaa
     4921 gaaaaacagc aaaaaataat tctaataata caaaaaataa gataaagcta tgcatgaaaa
     4981 attcttctgg aagtacattg atgattggat cagtaacggg atcgaataac cagtcatcgt
     5041 tgttgaaaaa cgtttcatga aaaaggataa aaaagcgatc aaatcctatc atcatgaaaa
     5101 aaccgaaaac aactggcagt aacatgccga tttgaaacgg acgaatcaac cgccaaagcc
     5161 gatcattctt ttgcaaatat ttgataaaca aaatactcgg gatcaaagtg atgaaaaaca
     5221 cagcataatc cactaaaaac aaaattttca cttcataaaa gtgatgggct ccgctagcag
     5281 aaactgggaa atctggcaag ctgaggatgg ttttgaaggg attattgaga taattcatta
     5341 ggtgatcaaa atttttcagt aacgtctctt gatctaaaga agtataatcc aaaatttgca
     5401 aatgtccgat atcaaatacg tatagtggtc gaaagttgat cgtcacacca actgccagac
     5461 tgatcagtgt cagaaatagg ctgaacaatc cagcatattc catccactgc catctcgaat
     5521 ttttcatatt agaagctcca ttcatccagt gattgtttca cataggtagc tgggataggc
     5581 aacccaggta catcttcttc ttttgtaaaa cctgataaaa cgagcaaagt atctatatta
     5641 ttgcgtatcc cagcttggat atctgtttca tagttatctc caaccatgat tacttcttct
     5701 ttttgcagcc ctaatacttc aacggcttta tccataatga tggcttctgg ttttccgatg
     5761 tagatcggcg gcgtttgggt tgctgtctct acaaaagaga tgactgaccc tgctccagga
     5821 agaaggccgc gttcagtagg tatattctta tctgggttcg ttccaatgaa agtagcacct
     5881 ttttgaatag caagtgttgc tttaactact ttttcatagg tcagataatt atctaagcct
     5941 accactacat agtcaggagc ctcttcttcc catgtaaatc cggcagctaa aatcaagtca
     6001 atcaatcctg cttctccgat aacatagact tttttccctt ttccatctgc tttcataaaa
     6061 tcgattgttg ccaaagtagc tgtgtagact gtctctggag ctacatgaat gtcaaactca
     6121 tctgctagac gattagctac tgtttctggt gacttagtcg tgttattggt aacaaacaag
     6181 aaaggtaatt ttcttttttg caattcttcc acaaatcgtt taccggccgg tatgggttgt
     6241 gtcccacgat aaatcgttcc gtctaaatca attaaatatc ctttatagtc cattcatcca
     6301 ctactcttcc ctttttctga tcgtaaatgc tgtttttgtt ttttctactt gtttaggttg
     6361 ttcttgtttt tttacttttt cagaggtttt tgctgactct gtttttcgat ttttaataac
     6421 cggttttttg cgattagatg cgactggtcc ttttttctca tcgatatacg cacgattatg
     6481 gttttttttc ttttttcggc gctgagttgt cttttctttt ttaccgccga ttctctcgat
     6541 gacaaaatac gcacaaccaa aattgcaata ttcgtaaaga tagtcttcca aggaatcgat
     6601 tcgctgatct ggatgcgccc gtcgattatc cgcttggaaa aatcctttta ggcgtagttg
     6661 ttcataaccc caatctccta cgatataatc atatcgggct agtacatcac tgtaacgttc
     6721 tccaagcttt tctgcattaa atccttcacg ataatcggac acaagccgat aatgtctttc
     6781 acctatcaaa aaattactgt catctaacag tgtcactagc tcgcctttga ctttttctgc
     6841 tttaggctcc tcaacgattt cttccaaagc agctgtcagt tcttcggtga ctgttgtttc
     6901 ctgttcagct gatgattttt tcttagcaat ttctgtttta tgctcaacgt tctgctgttc
     6961 attttgtttt gcagacatgc gttcaccacc tatctttttt attatacctt gtttttattt
     7021 gattgggtag tgagctttca agtaatctcc taccacactt cggataaatt cagggaacaa
     7081 aaattcattt tttccagcaa tctcaatcgt tggaaaaaat ggaacaaaca tcaaatgatc
     7141 tacggtagta aaagtataat ttttattcag atcgattggc ttgttcttcc aaaagacttg
     7201 atggttaata gaatcatatg tgattccatt atagaccatc tgcccaaaaa tcttaccgcg
     7261 aaatcccatt cccttcatag gatagttacg taaaaagtta cgattttttt ctatttctaa
     7321 aactaagcgg atcagatcat ttccacccaa tgtgacattt aacaagtgca tcggatgcgg
     7381 caacgcagtg tgaagctgat cctgattgac ccgccctttt ggaatttcag ttaaaaacag
     7441 accactgttt aatattgctg cttccgtctt tcctcgttct ttcaccgcct caaaggctac
     7501 ttggataaac gaatgctcat tgaagatatc tagactcaga gaaaatggca aatctgctac
     7561 ttctttttct tttaataatt catgcccacg ttctagataa ctatttattt cttgttcatc
     7621 ttctgtaaaa gctgtcattg tttcggtagg gatggctttg gctgaatgct taaggatttt
     7681 tttctcttca tcgatggtta aatggacttc accgacatac tgcccgtact ttccagcggc
     7741 tgctatttgg acatcattga ttacgtgacc ttctctgaat aaatgatggg tgtgcgatcc
     7801 taaaataaca tcaattgctg gtatttcttt tgcaatttgg aaatcatcct gaatcccgag
     7861 atgactcatc aaaactaaaa catccacttg tgaacgtaaa tcttcgacta attctggcaa
     7921 aatatcataa ggattccgta tgtcccagcc attcggatta taagtaagcg gaaatggagc
     7981 agtaaatgca attagtccga ttttcgtctg gtgtttcgtt gtaatgattt tatatggttt
     8041 cgtccatgcc ggtggctgga gcgtattttt atcgaataag ttatccagga tgatatcaaa
     8101 ctcagcatga tcgtataaat gattcaactc ttctttggaa tttcctacgc cttcattgtt
     8161 tcctatcgtc gctgcatcat aaccaacttc attcatcagc aagatatttt cctgtccatt
     8221 cgttgcttct gacaatggat gccaacgatc cacaaaatca cctagatcta ctgcaatcgt
     8281 tgtcgttttt tctttctttt cgttttctcg tttacgttga tccaaaaacc ttctgatcct
     8341 tggccaattt tctaaatgag aatgcagatc attcgtatgt aaaatcacta ttttttccat
     8401 gtaccctctc gcctctcttt atctttatca taccataggc agctttttct tccattgaaa
     8461 caagctctat ttcagagttt ttcctgcctt gaagcaaatt gtataagtac aatattcgat
     8521 aaatcagtct ggctaaaaaa tcaggaacat tcatttttac agtactataa aaataaaaaa
     8581 aacagacccg aaagtctgtt gtcgtttcat tataatcaga cacatggcgg aacctgctta
     8641 gtgctgcttc cttccagacc tgacacgctt cacaagccca ccattgtcct agcctttcgg
     8701 caaagaatag tataccttat ttttcattcg ttgaccagtc tttttttaat ttcttttttc
     8761 gctggcggag ctttcggaaa aagtcagtca aaatcatccc acattcgtct tctaaaatgc
     8821 caccttctac atatgcttgg tggttaaacc gctcgtcctc caaaagattc atcaaggtac
     8881 cggctgttcc gccttttgga tcatacgcgc cgaaataaac ttctgctaca cgagacaaaa
     8941 tcatcgcgcc gctgcacata ggacaaggct ctaaggtaac aaaaagtgca gcatcttcca
     9001 agcgccagct tcccactttt tcacaggctt ccttgattgc aagcatttct gcatgtgtag
     9061 tagcatcttg cgtggtttct ctaaggttat atcctcttcc aatcactttt ccatccaata
     9121 caaccactgc tccaatcgga acttcatgaa gcatttctgc tttctttgct tctgccaatg
     9181 caaggcgcat ccatttttct ttttctactg catcaagatt tgtatttacc aattaccctt
     9241 ccctgccttt gttttttctt gttttacttt agcatatgat aaaaaggctg taactaactt
     9301 ttttttctat ggtaaaataa atacaagagg tgaacaagat gaaaatggat aaattacttg
     9361 ctctttaccc acaagcacag aaaaaaaact ctccagcatc agaagcggat atcctttctt
     9421 ttgcaattga tgatgctttt atctggatca aacaagattc attatctcaa caagaaacca
     9481 gccttttgaa agcgttattc cctgtaatca acgatcaaaa aaaacaccca tggtatcact
     9541 atctttttaa agatgcttct tgtgttcaag aaaaaagctt tcgtttgatc cagcttcacg
     9601 tcgaatcaaa aggagaattc ttaaaaaaag aatggcaaga tactattttg gaattatttc
     9661 gctattctgc tgatttcttt ttttatacag acacagatgc gctcttagtc gaacaaataa
     9721 accgtgatta tcttgatgct actgaaatca acggtatttt tctatcttta gatgctgatt
     9781 ttgatacgac aacgtctgta ttcgttggtt ctttccattc acctggcacc gacctcgtcc
     9841 ctttgtttgc tgaagaaaga aaaatttttt taacagagca aaacaatctt tatactcaca
     9901 gccgtacttt ttctatgcaa gatgtggctt tacattacta tacaaaagat actgtaaaag
     9961 atagtaagtt gatcaaaagt tacatagaac tgatcaaaca aaatgaaatg gaagtgatca
    10021 ttcaagaatt atggaatgaa ttaggcaatg tcagctctgc tgcaaaaaca ctgtttcttc
    10081 atcgtaatac tctaaaatat cgaatcgaaa aatttcaaga acagtctggt ttcaatctaa
    10141 aaaaagccaa cgatttactg ttttgctatc ttttattgct tcatgaacat aattaaagat
    10201 agattaaagg ggttgtgaca ataattttgt cacaacccct tatttcgaat gaatagtgtt
    10261 caaaagctag tttctcttac atcatttttt caacaaaatg cattagccgg tcttttacat
    10321 cttcggttga atctttgatt gaatcaccat aaacagagat tttctttttt cctgatttga
    10381 tttttgctaa cattcccatc aaagaatcca agtcactatc tgaaagacca tctacgattt
    10441 ctagtaattt ttcatttcca tcaaatttgt catagacgaa tttttttact ttcgtccgag
    10501 tagtcatatg atggatcttt tccattactt ttcccgatac aacgactgct gtcgctacac
    10561 tggcagcggc tgctacgctt aatccgattg ttacttttgt tgatgttttc ataagtatca
    10621 gctccttttt atctatctcc atcatagcat agacaaaaaa ataaacgaac taatccactc
    10681 agtgaaatct caatccttta gggaaaaagt tctttactgt tccttttact tgtttcccaa
    10741 atccgacagg tatcttatct aatactagca aaacccatcc ttgattccca tcacgttgaa
    10801 atgtttctcc tgctgtgtaa gattgccatt ccttttgagt gatcggcaag caaggtatat
    10861 tctctatctt ttttgtagca agcgctaatg catagctagg ttcaaatcga ttctttttga
    10921 aatctcctaa atgcaaacct gtacgtacga cttttaaacc atcgagagaa ggggctaact
    10981 caggaacttc ccaaagatgg tcgttgaaca ctaaaagtct tccagtagct tcataatgaa
    11041 aatcatttga aaactccgtc cagagttttt cctgctcttt tgtcatttgc actttacttt
    11101 tttttcgtgt cttcttttct ttatgcatct gattttgtcc atgaaaagtc agctttgcaa
    11161 caaaatgtcc ttcaccctga tctttatgag gccatatacg tattgttttt tccaaaccag
    11221 ctacgcttcc ccattcagaa cgtcctgaac taacggattg agtcaaagga atctcctcaa
    11281 tcgttactgg ataattttct acaagccagc taatgatctc ttcattttct tctggtgcaa
    11341 acgtacaagt cgaataaatc agttgtcctt tattttttag catttttatt gctgaagaca
    11401 aaatctcttg ctgacgtttt tgacagtata gcggagattc ttctgtccac tcttttattg
    11461 catttggatc ttttcggaac attccttctc ctgaacaagg agcatcaacc acgatccgat
    11521 caaaaaagcc cgaaaaatga gggacaagtt ctgcgggtgc atgatttgtc actattgcgt
    11581 tcgaaactcc ccatcgttct atattttctg ataaaatctt cgcacgtttc ggaaagatct
    11641 cattcgtcac taataaccct ttccccttca tttgagcagc taattgggtt gatttaccac
    11701 cgggagccgc acacaaatcc aaaacttttt caccgggttt tgctgcagca gctgttccaa
    11761 cgatcattgc acttggttct tggctatatt catacccagc ttgatggagg aaagactttc
    11821 cattgacagt gccaagaaat ccctcattgc tgtagggagc gggttgtaat tcttcgctat
    11881 ggtaagtttg gaccatgtcc aatcctgccg gttttagtgg attccaccga aatccttttt
    11941 tcacgcttcc ttgttcaagc gcagaaaaaa aatcggatgc ttcttcgcct aaaagaagac
    12001 gatatttttt gataaattgc tgtggtaacg ttgtcgcttc ctccttcatt ccaatgtttc
    12061 gttagacatt ttacaatctt ttttacgaaa aataaaggtg gctgatctac tttttcatgt
    12121 tgctgcctaa ggtgactgtt cctttaagat attttccaag tattcatccg tcacctcgtt
    12181 tttatcccaa acagcgttca tcatttcaag aaaatcttga gaagaatcat ctgagcgcaa
    12241 acttcccaat gcattaagcc ataccaattc aagcgacttt tcttctttga tcttcgtgtg
    12301 acctttgaca tatgagaaaa atggccgctt aaatagcgaa tccatgtcta aatcagattt
    12361 tgcaatgatt tcacgaatac tttcatggat agaaaaaaaa tctttcaata agtagacaat
    12421 gcttaaaaca tcatcggaac tgcattcagc taagtagttt tcaaaataaa cagtgatttc
    12481 ttctaatatc ttctctttta acccgctgat tgatttgaaa tgtgtataaa tcgggtatgt
    12541 agaacaaccg cagaactctg ctaactctct tgcagtaatt gacttcattc catattcttc
    12601 cacgtatacc atacttttga acagcaaata ttctttacta taatttgtta ctctccccat
    12661 cattcttacc tccgtctctt
//
LOCUS       DANKHL010000081        12405 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000081 DANKHL010000000
VERSION     DANKHL010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.084"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            77..1960
                     /locus_tag="PW101_001565"
     CDS             77..1960
                     /locus_tag="PW101_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL2513967.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2035..2319)
                     /locus_tag="PW101_001566"
     CDS             complement(2035..2319)
                     /locus_tag="PW101_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL2513968.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(2309..3043)
                     /locus_tag="PW101_001567"
     CDS             complement(2309..3043)
                     /locus_tag="PW101_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDL2513969.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            3172..3795
                     /locus_tag="PW101_001568"
     CDS             3172..3795
                     /locus_tag="PW101_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDL2513970.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            3987..5108
                     /locus_tag="PW101_001569"
     CDS             3987..5108
                     /locus_tag="PW101_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDL2513971.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            5115..8243
                     /locus_tag="PW101_001570"
     CDS             5115..8243
                     /locus_tag="PW101_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDL2513972.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            8262..11456
                     /locus_tag="PW101_001571"
     CDS             8262..11456
                     /locus_tag="PW101_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HDL2513973.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(11602..12309)
                     /locus_tag="PW101_001572"
     CDS             complement(11602..12309)
                     /locus_tag="PW101_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2513974.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         4225 a         2196 c         2625 g         3359 t
ORIGIN      
        1 acgattctac ttgctatttt cacttaatac ccgtatatta cgaatgaaaa aagtttatga
       61 aaagttggaa tattgaatgg aaaaacaaaa aaggttaaaa aacagccgct acatcactgg
      121 gtttgatggg ctccgttcat tagctgtggt gggtgttatc ttctatcacc ttctgccaac
      181 tagtatgaaa ggcggctatt tgggcgttcc gatttttttc gtcgtatctg gttatttgat
      241 cacggatctg ctgcggcagg aatgggaaca aaatggaaaa atcaatattt ggcaatttta
      301 tgtcagaaga atgaagagac tctatccggc acttgttttt atgcttgtga cagcttcggc
      361 ttatatcact ttgtttcagc gagggctgtt aaacaatctt cgaggagttg tggtcagcag
      421 cttactgtat gtcaacaact ggtggcaaat caataacggt ttatcttatt tcgatcgatt
      481 tgctaatgag tcaccgttta cgcatatctg gtctttagca gtagaaggtc aaaactattt
      541 ggtttggccg atcttgtttg tattgttgat ggtatttgtt cgtaaaagaa aatggatctt
      601 ttatactgtg ttaggtacat ccttcctctc cgccatcttg atgatgatct tgtatacacc
      661 aggaggcgat ccaacccgtg tttattacgg taccgacact cgtctgtttt ctatatggtt
      721 gggaagtgcg cttgcgtttg tttggccaag tacgcgttta aagaaaaaca tcccgaaaca
      781 agcgaaaaat attctgaata tcgctggagg aatctcgctt ttggcactga ttctttgctt
      841 cttcttttta gatgatcatt acaatttcat ctattacgga ggaatgctcc tagtgagtct
      901 gttatgcgta gtgctagttg cagtgacagc acaccccgga gcaagtttga atcgatggct
      961 gacaaatcca gtatttactt ggattggcaa acgaagctat ggcatttatc tgtatcagtt
     1021 tccagttatg attttttatg aagcaaaggt gactgatatt gccgatcatg ttttgttgca
     1081 tactttagtg gaaatcacat tgattctaat catcagtgaa ctttcttatc gatatgtgga
     1141 gcgtccgcta gcacgttttg attatggtcg tacattccaa gtgatgaaag gatggttcag
     1201 caaaccgatc atcagtaaga gcaaaccatg ggttgttcca ggcacattac ttgtagcaat
     1261 tgcactcgtt ggtttcgtta cggcaccgaa aaacgctgta acagccgatc aaaaacaact
     1321 gcaggcaaaa atcgctgaaa gtaaaaaatt agcagaagaa accagaaaca attcagaaaa
     1381 caccactagt tcggttgacc aagcgatcct tgataagtac gagttgtcca aggcacaagg
     1441 gaataaggca caaaaattag aactaacggc atttggtgat tcagttatgt tggatgctgc
     1501 aacggatcta caagaacttt atccaaaagt agtggtagat ggagatgtcg gacgacaatt
     1561 atatgccagt gtaccgtaca ttgaaaaatt aaaggaacaa gatttgttga aagacactgt
     1621 tctgattgga ctaggaacga atggctcatt ctcggaagct cagtttgata gtgtgatgga
     1681 tgcaatcggt gatcgaaaag tatactgggt caatgtccga gtaccgacga agcgctggca
     1741 aaatgacgtg aatgcgatgc ttgaacagat gacgaaaaaa tacgacaata tgacgctgat
     1801 tgattggtac agctacagta atgaccatag tgactggttt tatgatgatc aagtccatcc
     1861 aaatcctgaa gggatgacac attacattca cttagtcagc gaagccttgc taggaggaag
     1921 ttcaagtacg aacagcagtg agacaaacgt gacagaatga tagctacaaa aaacttcagg
     1981 caaaaacatt tgtttttgcc tgaagttttt ttatatttac cgtgttttta acattcatat
     2041 agattcagga agcaaaacgg ttgagtgtgt ctgtaaataa cgttcttttt gttttcgggt
     2101 gagttttttg aatgcagctt ctgcttttgt tgcttcgctt cttgaagaaa atacttctgc
     2161 atggatcatc ttaacaggtc ttcttttttg aagacgagta tatttagcac cagtaccatt
     2221 gttatgttcc tctaatcgtc tagaaagatc ggttgtatat ccaccataaa atgtctgatc
     2281 tttacagaat aatacgtaaa aataatgttc atttgccata gagcatctca cgaatctctg
     2341 cagtgtattc tccatccata ttgtaagtga tcaatggtgg agcgactttg aatccgtcta
     2401 atttgccttc tttgattcct tcaactaaca aaatatttgc ctcttttcca ggttttgggt
     2461 aaacaaattg gatcctttta ggtgcaattc gatagtcttc catgacctga atgatttcta
     2521 agaaccgatc aggacgatgg accatagtaa agcgtccatt tgtttttagt agcttacttg
     2581 aaatatcgat cacttcttct aacgtcgtat gaatctcatg acgggcgatt gccaggtgtt
     2641 tattagggtt cgttttattt gttggcagcc ctttgaaata aggtggatta cagacgacta
     2701 agtcacaaga attatgtcga acgacagata agctgtcttt cagatcgatg gtgtggacag
     2761 tcatttgttc atttaaatgg ttaagcgcga tactacgtct tgccatgtct gctagacgag
     2821 gttgaagttc gatagcgtcg atagccgcct gggttcgttt acttaaaaaa agtccaactg
     2881 cgccatttcc tgcacaaagg tcaacaatct tccctttttt aggtacacgg gaaaagtgag
     2941 caagcagcac agcatctaaa gaaaaagaaa acactttgga actttggata atcttgatat
     3001 cttctgcgta taattggtcg attcgttcgt cttcgtgaag catggattcc ctccaaaatg
     3061 aaaaaaatag tcgtctgtat tatactattt ttattaaaaa gaaccaaggt ggcttgtcaa
     3121 acctctattt tttatgcata ctatgaccat aagaagggaa aggaagagac tatgttttat
     3181 cgatttatgc gcggggtagt tcgagtacta ttagccttag ttaatggaaa tgcccgctat
     3241 gagaataaag aagttttacc gcaagatgag aattacatac tagttgctcc ccatcgtact
     3301 tggtgggacc cattatatct ggcggtagct gcagcaccca aggaattcag ctttatggca
     3361 aaagaagaac tgttcaaaaa tccagttctg cgatttatat taactcgatc caatgctttt
     3421 ccagttaaaa gagaaaatcc aggaccaagc gtcattaaaa cacctgtaaa gattttgaaa
     3481 tcgacaaatt tgagtttgat catgtttccc agtgggacaa gacatgctac agaacttaaa
     3541 ggcggaatgg cattgatttc cagaatgtct aaagcgaaaa tcgtgccagc tgtatatcaa
     3601 ggtccgttga cacttgggga tctgttcaag cgtcagagag tgacagtgcg tttcggcgaa
     3661 ccaatcgact tatcagatat caagaaaacg gataaagaag gtatggcgga gatcgaacga
     3721 cgaacacaag cagcttttga caaattggac aaagaagtga accctgattt caaatatgaa
     3781 gtcaaaaaaa aataaaatga taagtttatg cgtgggcggt cttttgaatc tactgtcctg
     3841 ttcagttcag actttttcaa tgaaaacgag ggagagacgt caagtctgcc ctctttttgc
     3901 gttccaatat gtaaaataga agacaggtgt catgtaatgg atcaaagtgt tataataatt
     3961 agaaggacat tttttgaggt gattgaatgc gttttttaca tacagccgat tggcatatcg
     4021 gaaaaaaact acaaggttat gacttgattg aagaccagcg tcatgtacta tcttctatac
     4081 tagatatcgc gaaagaagaa aaagtagagg caatcgtgat tgccggagac ttatatgata
     4141 ggagtgtccc agctgttgat gccgtagaat tatacaatga attgatggtc gattggaatt
     4201 taaaggaaaa gatcccgatt tttgctatat caggaaatca cgatagcagt acgagacttt
     4261 cagcgggtac gccatggttc agttattcga actatttttt gcgaacacag ctagcagaag
     4321 catttgaacc agtcgagttt aaaaacgtac aatttttctt tcttccttac tttgaaccaa
     4381 ttgcagcgag attatatttt gaagatgaca atatccgaac gatcaagcaa gcggtagcca
     4441 aggtgataga taaaatgcag gagctatttg attcagaaaa aaaacaagta ttgattagcc
     4501 atttttttgt aatgggaagt gtaaaatctg attcggaaac gaaaatcgaa gtaggcgggt
     4561 tggattcgat ccctggtgaa ctacttacac cgtttgatta tgtagcacta ggacatttgc
     4621 ataatcaagc tgctttgaga caggataatg ctcgctatag cggttcccca ttgaaattct
     4681 ctttatctga gatcaaccaa aaaaaaggcg tatggatcgt tgacctgaca acaggcctgg
     4741 agttgaattt tagggagatc gaacccctgc atgatattga agaaataaca gcgagtttca
     4801 aagaattgct ggatcctgcc ttttatcagt ccgtcaatcg ggagaattac atctatatct
     4861 atctcactga tagaacgatc atcccgaata tgatgaacca gcttcggcaa atatatcctc
     4921 gtattttggg gattgaacgg gcaaacggtc gagcagaaca aacaaagcgc aaacgaaaag
     4981 tactagaaat gaaagatcct caaaaattag cagaagcatt ttttagtgaa atgacggaag
     5041 agcagctaac tgcaaaccaa gaaaaatggc tcggagaaac gttgcaagaa atcatggaaa
     5101 tggaatgatc aagaatgaaa ccgaaaaaat tacaacttaa aaattttggt ccttttatca
     5161 atgagaccgt agatttttca cgtttgacag aagcaccgct ttttttgatc agcgggaaga
     5221 caggagctgg caaaacgacg atttttgatg ggatcacctt cgcacttttt ggtgaaactt
     5281 ccggaagact tcgctctgga aaagagatgc gttctttgtt tgctacacca gacgaagaaa
     5341 caagcgtgac tttttctttt gaacatcagc agatgagcta tgaaatcgaa cgaaaaccag
     5401 aacaagtttt agcaaaacga aaaggaaacg gaacaaaaaa acaggcagcc aaagtaacgt
     5461 tgaccatttt tgatgggaaa ggaaaagaaa taaaacaaat cacaaaacgt acagaagcag
     5521 atcagttgat cagagaactg atgaatctcg atgcgaaaca gttttctcaa atcgttttac
     5581 taccccaagg agaattccgt aattttttgg tttcatcaag cagtgataaa gagactgttt
     5641 tacgtcattt attcggtact caatttttcc aacagttcaa tgagcgatta aaagaaaaag
     5701 caaaacaaca gcaacaaaaa ttggatcatt tagagcaaga gctgcatctt ctgcaaaaaa
     5761 ggtttgtttt agtcaaagaa gaggaaccag cagcagggtt tgaagccgtt ttagcccaat
     5821 ggacaaacca tcaggcaata ctcgaaaaac aaatcacgga agaaaaagaa gctctaacgg
     5881 aacttcagga aaaacaaaag caactagaaa attcctacta tcgttttgaa agcttgaaaa
     5941 aaagttatga agaaaaaacg atattaataa acaagcaaaa ggaattagct gctcaaaaag
     6001 acgaaataga ggaaaagaaa cgatggattg ggtattatga atttacgcaa cgattagtcg
     6061 aaccaatcca gcgcctacaa gaaataaaag ttgaaaaaat agaactgtca caaaaaagta
     6121 gtgagcaaac aaagaaacta gcgatgacta cgcagaatta tgaagagtgg caaaaaaatg
     6181 aagcaaaccg cgtacgccga caaacaaaaa tcaaagaagc tgctcaaaaa ctgcaaaaca
     6241 aacagatgat ggtgccaatt gtcgaatcct tacgtagtaa aaaacaagac gcagaacaat
     6301 taacaatttc attggataaa gaagaattga atgccgagaa gctgtatcaa aatctcgcta
     6361 tctatcagga aagaactcga attcttcaag aaaatttact ggatcaggaa aaactgcaag
     6421 aagatcgtct tgcttttgga cagttaaaat atctagatga aaagcagcaa gaaatagaaa
     6481 aacaaaaagc aaaagacact gggtcgctcg cacagataag agaagaactc aaaaaagcaa
     6541 ctgaagaaat aacaaaaaat agtcagctat tgaaagaaca gacagaacaa gcagaacagc
     6601 tgaaaagcca atgggcaaag cagcaaattg ctcgtcttca gctttttttg cgtccagggg
     6661 agccatgtct tgtttgtgga tcaacggaac accccataca acaattaaaa cacgaacagc
     6721 caaataagga agatatctta aaagtagaac aacaactggc agaagctgag tctgctgtag
     6781 aaagaactca gcaaatcatt gccaaaagtg aagcacatga aaataatctt caacaacaat
     6841 tatcagattt agaacaaaaa atcatggcaa cgaatataca attggctgaa caagaagaac
     6901 acttgcaaca ggctttgaaa aaggcatttc ctgagataac agaacaaaaa acaaaaacaa
     6961 ctcttgtagt aatcgaacaa tctttagata gacgaaaaag caagatggcc aatgcaagaa
     7021 tagagctgga agaaataaga caacaagcag aaaagctcga acaacaatta gctgtctaca
     7081 aacagcagca gacagaaaaa aataatcagc tccagcaact gcaaggagaa atcaaaagtt
     7141 tagaacaaca attgcaagat acggatagta actgtttaga agaggagata cgacgacttg
     7201 aaaaacagtt agaagaagac tcgaatcaac tagctgttga gcagaaagca ggagtgcaat
     7261 tgttgcaaga tcttcatacg cttcggacac agcttgatct tcaacatgaa caaatagaaa
     7321 aaatagatga aaaaatagaa aaggaacaag aaagaatagc taaaaagtta gaaagccagt
     7381 cgtatttttc aacaattgaa gaaatctgtg catttgcaca acagcaagca caatataaag
     7441 aaaaccaacg tacagttact tcttatcatg aagagcaatt agtgacagct gaccggctgc
     7501 gtcaattaga aaatatccag ctaactgaga cattcccaga tacagaagag atcagccaac
     7561 agttagaaga aataagacgt atgatcgaaa taaagcaaga aaagatatac ggtttacaag
     7621 aacaaaaaat tagtaatcaa aaaatctacg atgaatgtct ttcgatttat cacacaagcc
     7681 agacgaaatt agatgagctt acgcaattac agcagctttc gcaaacattc aacggagaaa
     7741 atcctaagaa gactagtttg gaacggtatg tgcttcaggt ttatcttcaa gaagtgttac
     7801 aagtagcaaa tgatcatctc caacgtttaa cgaaaaatcg ttatcaattt gagctagcgg
     7861 ataccattgg cagttatcga gggaaaaccg gattagaaat caatatctat gatgatcagg
     7921 caggtatgtc tcgaagtgcg cataccctgt ctggcggtga gagttttatt gcagcacttt
     7981 ctttagcatt agctcttgcc gatgtgatcc agacacaagc tggtggtgta gcaatcgatg
     8041 cattgtttat tgatgaagga tttgggtcat tagatgaaga agctttggaa atggcaatgg
     8101 aagctttaga aacgatcgaa agtgaaggaa gaatgatcgg catcattagt catgtaagag
     8161 aattgaaaga acgagtatta caacaaataa gaattgatac agaaggtagc ggacaaagca
     8221 aaatacgcta tcaacttgga taaaaaggga gattggtaac tatgaaatgg agtattccag
     8281 aaaaagtcat tgcacgtgga cgtcaatact tgcaagaagg ccgagtgtta tcggttgtcc
     8341 ctgaccaaga agaaaacgtg tggcatgcag aagtcttggg cagtgaattg tatatggttg
     8401 atttggatgg tacagcaaaa gagatagatt actgccaatg tccctattgg gaggaacata
     8461 aatattgtaa gcatacagtt gctgtggaac tttatttacg gtcgaaaaat ctatctcgta
     8521 tcatgaaaaa agaccagcca atgctagaac cggtaaaagc aacaagtgag ggagaaatca
     8581 ttgcaaaagg tttttctcgt ctaaaaacac aaaacaatcc tcaagcggtg caacctttga
     8641 ttattgaata ccaagtagaa acgattgaga ctaatcagta tcacccagag ctttctattt
     8701 tggcacttta cttgcgtatc ggttcattgg catcaccgaa aaaaacatat atcgtaaaaa
     8761 acatctatga ttttttacaa gcttacacag aaaaagaaac ttacactgtt aataaacagt
     8821 accagtttcg tctagataaa cgggcgtttc aaaaagaaga tcaaaaaata ctcacgcatc
     8881 ttgcaggaag tgctcagacg catcaattat taggtacgaa cggcttacaa gtcaaaggaa
     8941 aactagataa aagatatctg cttctcccag tagaacaagc ccgcttcctg ttagagcaga
     9001 tgaatcagac tacacgtttg catctgcaga taaatgaaca aaacgatcaa ggaatacaat
     9061 tttccgatca aaacaaacct ttatcattca aagtggaaaa atttggagaa gattatttgc
     9121 tgcaagcatt gaatgatttt gattttttct ttatgcatta tcagtgggga gttatccaag
     9181 ggacatttta ttctctgacg aaatatcaac aaacggttta tcaaacttgg aaacaattga
     9241 ttcgccgatt ggagaaacca gaagtagtgt tcaagaaaaa agaattaccg tctttgttta
     9301 aagagattat ccctctgctg tcagagatcg gcgacgtagc tattgcccct gcagtagaag
     9361 aggaagtagc agatatcccc gtcgaattta ttttcttttt ccgaaaagca aaaggaaaaa
     9421 tccaagctcg agtcgatttc gtctatggtg aagtcatcta ttcgactgac ccgaagcatg
     9481 aggtctcgac agaacaggct tttcaggtgt tacgtgacac agtagaagag aaacgagtac
     9541 ttgatctatt cagaatgtat cgctaccaga aaaatgaaac tggttatgaa cgtccattgc
     9601 caagcgggga agaactatat gcctttttcc gcacagaact atctgtcttc cgtcagtttg
     9661 gtgaagtcag acttggaaga aaattgcgag agttgtattt agatgcgcat aaacatcaac
     9721 cgaaaatcga agtaaccgag accggttcat ggctcgatat tcgattcgat gtgacaggga
     9781 tagaggagca agaaatcgat catgtcctgc aaagcttgct gagaaatgat gctttttata
     9841 cattagaaaa tggtcaggtt ctttcttttg attcagaaga atttcaacaa acaagccaag
     9901 tgttgcaaca attacgggaa tcgatacgta cagaagaagg aacgatccat gtacctaaaa
     9961 atcaaggatt gatcattcaa aatcaattgg aaaaaagcaa tgctactttt agtgaatcgt
    10021 tccaaacgat ggtacaagat ctcattcatc cagaaagata ccaggcacag cttcccaaag
    10081 gattgaatgc aacgatgaga gattatcaaa agcaaggttt tcgatggttg aagatgttag
    10141 gtcattatca gtttggcggt attttagccg atgaaatggg acttgggaaa acactacaga
    10201 caattgcctt tttactttcg gaaaaagaag aacggaaaag cttctctgct ttgatcgtag
    10261 cgcctgcgag cttaatttac aactggcagg cagaggtgag aaaattcgcc ccttctttaa
    10321 gtatccaagt aatcaatggg aataaaaaag aacgtgaaga attactggct aaagacacag
    10381 atatccgagt gacttcttat gccagcttaa gacaggatct agctaattat cagtcgcaaa
    10441 aaattgatta tttgattctt gatgaggcac aaatggtcaa aaatagcagt acgaaaactg
    10501 cacaagcatt gagagaatta gctgtacctc agcggtttgc actgagtggg acaccaatcg
    10561 agaataattt agaagaattg tggtcattgt ttgcaacgat tatgccagga tttttcccga
    10621 ctaaaacaaa attcagggaa cttgccgcag aagaaattgc gcaaatgatt cgaccgttta
    10681 ttttacggcg agataaacaa actgtgttga aagacttgcc ggaaaaaaca gagatgaatc
    10741 tatattctgc acttacagaa gaacaaaaaa ctgtttattt agcttacttg cgtcaaatgc
    10801 gtgaagaaat cacatcaatg gattctgaag cattcaagaa aaaccggatc gggatacttg
    10861 ctggtctgac aagactgcga caaatttgct gcgatcctcg tctgtttatt gaagattacc
    10921 aaggcggttc cggcaagctc gaacaagtaa aagatctact ggtagctgcc aaagagaaca
    10981 aacgaagagt tttgctgttt tctcaattta cagggatgct gactatcttg caagaagagt
    11041 tagccgagct agggatctcg acattttatt taagaggcag tacgaaacca caagatcggt
    11101 taagtatggt agacgcattt aatgctggcg aaaaagatgt ttttctgatt tccttaaagg
    11161 ctgggggaac tggattgaat ctcacgggag cagatacggt catcttgtat gacctttggt
    11221 ggaatcctgc cattgaagaa caagctgctg gacgtgcaca tcggatcgga caaaaaaatg
    11281 tggtagaagt gtggcgtatg atctctgaag gaacgattga ggaacgcatg gattcactcc
    11341 agcaagaaaa acgcgaactg ttccaaaaag tgatccaagg taatgaagaa cagttgacga
    11401 aactgactga agaagacatc cgcatgattt taagtgtagg agaaatcgag gagtaagaga
    11461 ctcgaaaaag cgttcagtaa tgggtaagaa agcctttcta aagaagcgat tgtttatttg
    11521 atttaaaaaa tagcaaggag gctgggattc cattttatcc cagcttcctt gctatcattt
    11581 ttttatttct atttttaaag gttatacaaa gattgtcttc attgtctcta acagctgatc
    11641 aaaagaagaa atctcgatat cgcaaattgg agtctgttca attttgcgtt ctcgatggtt
    11701 gaaccaaagc gcttgccagt tagcactttt tgcacctaga acatcattgt cgtaattatc
    11761 gccaacgtat aaggtctctt ctggaagcat atgaaattct ttctcagcca gctgaaaaat
    11821 ctctttttct ggtttttgga aacctgttgc ttgagaaatg agcatattgc gggaaggaat
    11881 ccaattgttc aattgaagtt gcttcagttt ttttgtctga tggtcagtcg gcccatttgt
    11941 gataattcct aatgggacat tcttttcctt caagaaatcg agggtttttt tgacctcctc
    12001 atgcaagcag atattatcaa gttcttcctc gtaaattttt tgaaataaga ggccatcttc
    12061 ttccgtaata tgagggtagt ctaaatcttt tagtgattgg ctgatccgat gtgctcgcat
    12121 atattctagt gtccaatctc ctgccattac ttttgggaag ttttcatcac tatgatggcg
    12181 gaaccggata tatagtggat gcatatctgc atctgaaaca agcggaacga ctcttttgac
    12241 cgcgtttcga aatggctgct gctgatcata catcgtatca tcaacatcaa acacaactga
    12301 tttaatcaaa attttacacg cctttcggtt ttcattgatt ttttcacaaa tccttgacta
    12361 ttgtaacaga aaaaattgtc taaaaaagct gataaataaa ggttt
//
LOCUS       DANKHL010000082        12226 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000082 DANKHL010000000
VERSION     DANKHL010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.085"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..566
                     /locus_tag="PW101_001573"
     CDS             <1..566
                     /locus_tag="PW101_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDL2513975.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
     gene            967..1572
                     /locus_tag="PW101_001574"
     CDS             967..1572
                     /locus_tag="PW101_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HDL2513976.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1588..2160
                     /locus_tag="PW101_001575"
     CDS             1588..2160
                     /locus_tag="PW101_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2513977.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2412..2669)
                     /locus_tag="PW101_001576"
     CDS             complement(2412..2669)
                     /locus_tag="PW101_001576"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513978.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2795..3610)
                     /locus_tag="PW101_001577"
     CDS             complement(2795..3610)
                     /locus_tag="PW101_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513979.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            4127..5452
                     /locus_tag="PW101_001578"
     CDS             4127..5452
                     /locus_tag="PW101_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL2513980.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5445..5795
                     /locus_tag="PW101_001579"
     CDS             5445..5795
                     /locus_tag="PW101_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513981.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5777..5962
                     /locus_tag="PW101_001580"
     CDS             5777..5962
                     /locus_tag="PW101_001580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513982.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(6866..7141)
                     /locus_tag="PW101_001581"
     CDS             complement(6866..7141)
                     /locus_tag="PW101_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HDL2513983.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(7113..8066)
                     /locus_tag="PW101_001582"
     CDS             complement(7113..8066)
                     /locus_tag="PW101_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2513984.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8678..10171
                     /locus_tag="PW101_001583"
     CDS             8678..10171
                     /locus_tag="PW101_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDL2513985.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            10306..10602
                     /locus_tag="PW101_001584"
     CDS             10306..10602
                     /locus_tag="PW101_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDL2513986.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10703..11554
                     /locus_tag="PW101_001585"
     CDS             10703..11554
                     /locus_tag="PW101_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513987.1"
                     /translation="MKSDDWIEHNWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11575..11858
                     /locus_tag="PW101_001586"
                     /pseudo
     CDS             11575..11858
                     /locus_tag="PW101_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4206 a         1872 c         2286 g         3862 t
ORIGIN      
        1 attattttaa gtctgaaaag gatacatcgt ctctaacgtt aactttagag gaaggagttg
       61 aacctgacaa ggtgacctct aaagtgataa accaattgaa agaaaaaggt agtttgaaac
      121 atttggggga atatgaagtg ttagatacgg ctatgttaac gaaaggaatt ggtcaaatcc
      181 tcagtacgat cacttatttt ataacagctg tagcaggtat atctttattt atcgcagggg
      241 taggtgtaat gaacatgatg tatatatctg tatctgaacg aacaaaagag atcggtataa
      301 gaagagccct aggagctact cgaaaatcga ttatgctgca gttcctttta gaaggtttga
      361 ttttaactct ttcaggaggg ttaattggct atttattagg aatggctctt gcttatagtg
      421 ttggttcttt gataaaggta aacgtgtcac ttgatctatt tactatttta ctagcggttg
      481 gggtatcttc ggttattgga ctggtctttt ctgtaatgcc cgcttcagag gctgcaaaaa
      541 aagatttaat tgatattctt agataaaatc agaatgacaa gaatatttac gttctagatt
      601 ctaaaataaa acacaaagag accaaccagg caccctattg tttctttgta gtatagagat
      661 attctttggc ttgtaagaga tcaatagagt taggtgaccg gagttcgaaa taaaggaaat
      721 ttttgattgt ataggagaat tctttatccc taatatttag aattttgagt atgccatgtt
      781 gatgttttag attctctata aatatagagg tgtatcaaag gtcaactaga ctgtgcaaaa
      841 tagagacact tgaaaaatct atcgggggtc agaaacttaa tagtttttga cacataaaca
      901 aaatcgcgat ataatggggt tgagagtgtt tcataaactt atgtcataaa gttaaggaga
      961 tttcgcatga ttttagagaa taaattggga ttaaccaatc aagttgagtt ggctaaggtt
     1021 gaagaaaaac tcagtaaaca aaaagcgaaa gaattgtacg attgtggaaa aataaatgaa
     1081 atcgaagttg gtacatttaa aggtttatct gaaattcatg aatttctatt ttcggatatt
     1141 tacgattttg ccggaaaaat ccgatcggtc aatattgcaa aaggaaattt tcgatttgca
     1201 ccagttatgt atttagaaca ttcgttacag catattgatc agatgccaca aacttctttt
     1261 gaagagatta tcaagaaata tgtggagatg aatatcgctc acccatttcg agaaggaaat
     1321 ggccgaagta ctagaatttg gctggatctc attttaaagg aagaattgca aaaagttgta
     1381 gattggaact taatcgataa agcagactat ctttctgcaa tggagcgaag tcccataaac
     1441 gatttggaaa tacgctatct aatttccaat gctctgactg ataaaattga cgatcgcgag
     1501 ttatatatga aaggaatcga tgtcagctat ttctatgaag ggtattctga gtatacgata
     1561 gatgatttgt aaaagtaggt ggaccaaatg acgaaatatg gatatgcacg tgtctcaacc
     1621 gcttctcaaa gtacaaaaga acaaattcaa cagttgcttg agaacggcgt agagaagaag
     1681 aatatttttt cagaaaaatt tacgggtaca aaacgggatc ggccaaaatt caatcagctg
     1741 ttggctggtc tgaagcctgg agatgaaata gttgttacga agcttgatcg atttgctcgt
     1801 aacacgagag aagcattaga gatcattgaa cctttactcg atgataacgt aacgatcaag
     1861 gttttgaatc taggaacgat tgaaaatacg tccatggggc gaatggtgac acggacgttg
     1921 ttgagtgtag cagaaatgga acgtgatatg attgtggaac gtacccaaga aggaaaaatg
     1981 tttgcaagaa agaataatcc taatttcaga gaaggacgtc cgaaagcaac aatcacaccg
     2041 aagaagaggc acgcatatga actgcttact tcaggtaaat cgtataagga agttgaagct
     2101 ataaccggtt tcagtcgcag tacgcttttt agaataaaga agaaaattga agaaagttaa
     2161 aaaagaagtc tcagcgaacc agacaggtaa atacttaatg cgttctagat atgagtctat
     2221 tagcatgttg tgaaatataa tattttttta tttacttgat ttctgctcaa aaaatgtgta
     2281 tggtaactgt aaaaggtaaa gtaattaaag gtatgctagc cacgcttact atattaggat
     2341 accatcatat atttataatg aacgatttgt tcgtctacca aacaacacat atcgttcagc
     2401 gtaagtcacg tttaggaacg tggctttttt agtgttatta tcgataaata atctattgac
     2461 tctattgatt ttatctccat atccctacgg tgttgattgt ctttagatct ttcatttaag
     2521 aaccaaataa ctatcataaa aatcagcact gctgctaata taataaacag cattcctatt
     2581 gtgatattac ctattgattt taccataact acgattaaag taataatggc attcaagtct
     2641 cctttgttct ctatatataa attagccata aatcatcttc ctttcaaatt aagatacttg
     2701 tttgatacca ataaatagta agcgtggcta gcataccttt ttctgtaata gcaagtgttc
     2761 taatagtcca aacttaatga tggaccgatg ctagttatag tacagtaatg ttttctttct
     2821 tatctagatt tttagtaagc tctatcagca aatctaacaa atctgagtcc ttttcagaaa
     2881 ctaaattaaa tctagtgttt acaacaaagt tttcatgttc tatattgttt agattatttg
     2941 cggaaaggag ggcttctaga tcttgacatt caaatctata tgcatcgtca taaatcttac
     3001 tagaaggagt gcttaattcg aaattactta ttttctcatc ttggaccaag ataaatttat
     3061 agatagtatc tttttgtgtt tttcttttaa aaacacaaca gagtgtgtta taaggaacgt
     3121 tataattttc atcagcctca ttttttctcc attcaaacaa atgataaact gcaaccaagt
     3181 tgccatcaat ttcatggaca gtgctctcgg taacatcctt taaagaacta tcaagggcta
     3241 atttatagta actagagaga ggaagtaaaa ttgtcgattt actgagatgg ttatcgtcta
     3301 tacgtgcaat catacctaaa tcaactaagg ttttattcca ggtgtttata gttttagttg
     3361 tcactcctag agactctgct atggtattga ccgaatgcca ggaatccccg ccttcattct
     3421 ttgccttaaa acagtaatac agatataaat taatagcttt gttattcggt aaatattttt
     3481 caagttctgt tggtagaata aagtaagaat ccttaatgtt ttttctgaac tgtctccagt
     3541 ctctgtagtt ttgtttataa agttgtgaaa cggtttttcc acctttagaa agtcctgcta
     3601 tcttaaccaa tcttattgct cctttcttga atctgtcatg atcatacagt attttttact
     3661 tgtagtccag caccttaata tattatccta tgtaattaat ttcgtactag atagtaatta
     3721 ttttttattt aattctctag tcacttacaa ataagttata taaactacga ttaataaatt
     3781 attacaaaaa ctattaagaa aacttctaca tacaactatt acaaaactat taagaaactg
     3841 aatactccta ttttttgttt ttaaggaaag ttttttcttt tttgaatttc ttattttttt
     3901 ctaatataac taagcaaaac tggataatac gacacttatt ttcaaatttt cacaagacta
     3961 atagtaaata tccatttaat aaagtgatac aatgtgttga ttttacaaat caacagaaga
     4021 agtagggaaa taacttacct atgcttcttt ttttgtacat ttaatcgatt cttctttaca
     4081 aaaacgaacg tatgtttgta taatgtggaa gaatggagtg ttatttatga tggattattc
     4141 aaaagagcca gtaaatgatt actttttgat cgatatgaaa agcttttacg caagtgtcga
     4201 atgtatagaa aggaatttag atccattaac gactgaatta gtcgtcatga gtcgagcgga
     4261 taatacagga tcagggttga ttttagcttc ttcaccagct gcaaagaaga agtacggtat
     4321 ttctaatgtc agtcgaccac gagatctacc gcaacctttt cctaaaacgt tacatgtcgt
     4381 tccaccacgc atgaatctct atattaagcg aaatatgcag gtaaacaata ttttcagaag
     4441 gtatgtagca gacgaagatt tattgattta ctcgatcgat gaatcgatct taaaagtgac
     4501 cagatcaatg aatcttttta cgactgaagg aacaagaagt caacggagaa agaagctggc
     4561 tcaaatgatc caggagaata ttaaagaaga gctaggattg cttgctacag taggtgtcgg
     4621 agataatccc ttattagcta aacttgcttt agacaatgaa gcaaaacata atgatggatt
     4681 ccttgctgaa tggacgtatc aaaatgttcc tgataaagta tggaatattc ctgaaatgac
     4741 agacctttgg ggtattggat ctcgaatgaa aaaaagactc aaccaaattg gtatcatgag
     4801 tattaaagac ttagctaatt ggaatccata cactattaag agccatttag gtgtgattgg
     4861 cttacagctg tattttcatg cgaacgggat cgatcgaaca gatatagcga tcccaccaga
     4921 accgacaaag gaaaaatcat atggtaattc acaagtgtta ccacgagatt ataccaggag
     4981 aaatgaaatc gaactggtgg taaaagaaat ggcggaacaa gtagctattc gtatcaggca
     5041 acataactgt aaaacaggtt gtgtccattt atacattggc acgtcgatct tagaaacgag
     5101 accaggtttt tcacaccaga tgaagatccc tataacagat aatacaaagg aattgcagaa
     5161 ttactgtctt ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc acgttggaat
     5221 aacctatagc aagcttgtgt acactgattc gcttcagctg gatttattta gtgatccaca
     5281 aaagcaaatt gacgaagaaa atttagataa aattattgac aagattcggc aaaaatatgg
     5341 ttttacgtcg attgtacatg cttcttctat gttagaaggc gctcgtagta ttactagaag
     5401 tacattggtt ggtggccatg caggcggcgc aggagggctt aaaaatgatt gattataatg
     5461 atcgtggacg tttaaagtgg gcaggttttt atttatctga tcatactgaa aaaattgatg
     5521 ccgacaatga acaacgagcc aatcaaaatt tggcaaaaga acaaatgacg actgaagagg
     5581 ttgcggaagt attgaacgct gcagtcataa aaaatagatc agtatgtatt caaaaagaag
     5641 aacgaaacgc tgaaggatac tatccagaag atattgttag caagatctta ggtagtgatg
     5701 agctggggat ctacgttgga aaaaacaaag ttcactacga cgaaatacgt catatcgaat
     5761 tttaccagga taataaatgg ggtcgattag aatgaacatt atccagcaat atgagctaaa
     5821 gtatataacc tttgatcagc tatcagaaga gatttggggt tatggccagc gtttgataaa
     5881 tgaggttggt gttgaacgct tctctttcta tgtagaagct gcagcaggat accataaata
     5941 cgaatactat attggctatt aaagtgcaat aaaattatat ttcttttatg ttttacattt
     6001 tgtatcaatt ctgataaaat tacctatagg taattaaaaa atataaaaaa actagttaca
     6061 accttaggac gttgtaacta gcttgaagca tactagagct aactaaaata gcactagaaa
     6121 gcaatgttaa acccgtccaa caaacttgat ttaacattga gaccagctta ttcagctaaa
     6181 tatagttgaa taagcttctt ttgttttaaa aaaacaaaag gtgtactacg aataacgcaa
     6241 gtaaaagaaa tactattaca accagtaggc tacaagtaag tattactaaa cattgcgtag
     6301 ttagaacctt tgctctattg tctaaagcca caaaattaga gatcagcttt atgatgctaa
     6361 gagcgagctg catccatgcg gacataggtt cttttgacac tattaattct cctttttagg
     6421 ataagggaaa ttgctagtag ttttttcatt agcaccatcc cccttcattt gattgagtct
     6481 agtgctgctg tgatagtttg ctgtttaata tgcttacatt cttattataa cataaatgaa
     6541 acaaacacat ggttagaggt cgtatcaatc aaaatagtgt ctcacaaacg atttattact
     6601 tactcctgct gactaacgga ctgttcttca gtaactgtaa ctatatgttt gttaaaacca
     6661 ccattacctg tagataaaaa ccaatatatt tactatactc actatattaa atatatttag
     6721 tatagtaaaa actagaaaca ataaaatttg tgaatgttgt atacccccaa acaatacaca
     6781 tattttgaag gattaggatt aataaagcta aaaatatagt aagtatacta aatatattta
     6841 ctatatctag tatacttact atattttaat cttttgatcg tctattatat ataatccgtt
     6901 cctcgtcgtt taaagtatca acataggcgt ccattgcaag tttgactatt tcatacatag
     6961 gaatatcttt tgcataacta agatcccgaa tggtttttag tacgggagga tctacttgaa
     7021 ctggttttct ttctttcgca gtgtactttc gttcccctaa ctcgacatta atatcatctt
     7081 taattgaaat atcttcagca ccaattttct tcttatcttt gttcgttggt aataatgcca
     7141 tttagtttaa tctcctttcc aagaggtagg atatgattgg ccattgagtc agcatattta
     7201 ggtgtataat caaatccttc aatatcacca tgttgttcat agtattcaat tctttcttct
     7261 aattctgtaa agatgtctgc aaaaatagcc caccatcttc gatctacata atcatttaat
     7321 tggataccat tctcaccata catttctaac cgatccgagc ctttaacaat gcttttaaaa
     7381 atgtttgatt caccatactt atcaattgtt ttttgatagt ttgcgtgttg agaggaacgt
     7441 cgcactgaga aaagaacagg caaaattcca atgatttgaa gatgagactc ttcaaaattc
     7501 gtgacaatcg gaactaaaac tttattaata aaatcttctg ttccttccat tgcgtaccgt
     7561 tttaattctt gcattgcaat aatataatct gcagcagtta agaatgcacg aaccacatta
     7621 tctgttgatg gtgggcagtc gataaagata taatcgaaat tttttctcaa ttcttctaaa
     7681 ggtttaatga aggccaatga tctcttacga cgatcaggat atttttcgga caagtattca
     7741 ttaagttctt cagatccagg agagccagcc ataatatata aatgtggact tacttgagta
     7801 atacctgaaa cccagtcccc ttttttgatt gctgcagaca tggatcgatc aaagctagta
     7861 atttgatacg ttgatccaat gttggaagta gtatttcttt gttggtcata atcaattagc
     7921 aacacgtctt tttcaaaaag ttgactagaa acaatcgcta ataaatcatt gtctgtagtc
     7981 ttaccaatgc cgccttttct acctgtaagc attacaactt ttcctagttt atctgatgtt
     8041 ttaaatgcag tccgtaagtt ttccatagtt ccctccaatt tcctatacta aatatattta
     8101 gtatacttca tatacttaat atattcgata tatctaatat actccaaatt gataagaatt
     8161 cagactttat aaggaatata gtggatatac taaatatatt tgctatattc agtatatcag
     8221 atatattcaa aatgtcaaaa tatatttgtg cgttttgcgt tgacaattta gagtgaaaag
     8281 aggtaaactt aattctatag aaaccacaga taattcacta tttacaaagt agcaaaagca
     8341 gtggtactct ttaaacagaa aaattacata aaaaaaagag acggaaataa ttgattttag
     8401 cagaatcaat tatttctgaa tcacatggtt aggacatgtg ttcacgctcg tctcaagaat
     8461 atgtaagtag ctaccaacta cttatattaa aagttgatcg gatacctttt ccgattgcct
     8521 ttattctacc aaaaaaaggc gagcaactca acagaagaac gtttatttag tgtacttaaa
     8581 ttaaatattc ttaaacactg aagagagaac acatactatg gctgagatta aagctaaact
     8641 gtgttctctt tttgtttgag aaaggacgtt acaatcgatg ttgagcgaaa tttattcagc
     8701 tattcttaaa aacggcttac gtacatacaa atatcgaaac agcaccatga aacctgcagg
     8761 tcatattgat tccagtagga gtggtgctat ctttgcgtac cgtagcaagc aattaatgaa
     8821 tgttgggcgt ggtatggtca ttacttcgga agaagctatt ttagagaacg aaaagaaact
     8881 aacgcattgg acacctaata cctatcgctt tggtacttat gcggatgatt atcgaacagt
     8941 ggttaaaggt cattctgaga aaaacttaag tcaaatcaac acatttgtag ttgatatcga
     9001 ttcaaaagag aaccatcaag gcgaaattat tttagcctgt ttagatcaag tgggctacat
     9061 gccaacgcta attttagaat cagatcatgg ttatcaagtt tattttgtac taaagacacc
     9121 aggctatgtc acaaagaaaa gcaatttcaa ggtgatcgat gtagcaaaaa aaatttcaaa
     9181 aacaattcgt ctacagttgg ctgaaaaatt gtccggagtg gatctaggat gtaaccactt
     9241 cggaattgct cgattcccac aagaaaataa tattgttttt aaagaaatga actatcagta
     9301 ttcttttgct gactggattc aatggtcgat gaagcttgaa tcagatcaaa aaacaaaaga
     9361 agatcgagaa cgaaaactaa ttgtctttcc tgagaaaaaa gaaattcgtc aagtagatga
     9421 accgtggttc gatatgttac ttcataaagc aaacatcgtc ggcggagaag gacgtcttgg
     9481 tcgaaataat gtgattttca cgctttcttt agcttattac tcttcaggtt atgcattgga
     9541 tacttgcgaa tacaatatgt tcacattcaa cgaacggtta caggaaccgc tatctgaagc
     9601 agaattagta aagattgtac gtagtgctta ttctggtaat tatcaagcag caagcagaga
     9661 attcattatt gaattgtgcc agcaatgggt ttcaccaacc ttaaaaaata atgatctgtt
     9721 cattcaacga cgtggctggt ggaaatttaa aaagccacga gatcaaagaa aatatagtca
     9781 taaaaatgaa tggaaagaag atctattggc ttatctaagc gaaaaaagtc ttgctagtga
     9841 gccttatttg aacctgtcaa aaaaagaatt ggcttcacaa ctaacgatgc ccaagcgaac
     9901 acttgataag atcttaaatg agttacgaga ggagaataaa gtcttctaca aggtaacacg
     9961 aggaagaaat ggcggaatga tcattgcttc agtcaaaacg ttgttcgcga gggttattca
    10021 actaagtaaa gaaaagagac tagattattc aacaaggatc acagaggtat ttggactcaa
    10081 cgagagctta gtagaagaga catttagaca attatcaacc cccttgaacc ctcttaaaga
    10141 agtgaatctt tttgaagtag atacaggttg aaaaataaaa ggtgcaaatt gccttataca
    10201 atatatctat gatacttctg taggtgttgc tccctttgcc tagctacgtg taggtggtat
    10261 acttataact gcgtatgcag tcttatgtat catggaggaa gttgtatgtc gttaaaaaat
    10321 tatgtctatt cacatcctgt taatgtgttt atcattggtc gtttgggatt atctgtggaa
    10381 gtgttctgtg agctttatgg ttttaagcag gggactttgt cttcttgggt taccagggaa
    10441 aaaacagttg cttctttgcc gattgagttt cttcatgggt tgtccttagc ttctggggag
    10501 actatggatc aggtatatga ttgtttgtgt gtgttagagc aggagtatat tgagtataaa
    10561 atagctaacg agttaagaaa aaggaagaag tatatccagt agtagttttt atttacttaa
    10621 aaaattggtt cattgagcaa cctatcgctg ctaaagtagc aaaggaaaac gttaaaggaa
    10681 agaatttagg gaagaaatcg aaatgaaatc ggacgactgg attgaacaca actggcttcc
    10741 tctgaaaaga aatagcaaac gaaagaaatt ttgggtattg caattatggc cattaggaaa
    10801 actttgtagt tacggggcac gacttattct tgatttaacc gtttactcga ttggagtatt
    10861 tggaacggct tattacttga taaatgatcc caataggatt gcagcatcag acatttttac
    10921 agtgctaatg acgttgattt ttgtcatgct attgtctatt tttactcaat tagtcatgca
    10981 atttaccagg caaaaattaa cagcaggaaa acttttcaaa agcctattcg atttaattgt
    11041 tgtaaatgca attctagcga ctttaataaa acaaattgaa caatctggaa gaacaacaaa
    11101 aggagtgaag gattttttat tgacgtttat tcactttgta aatggacaca tgtatttgtt
    11161 tgaagtgggt attattctct tttttgtcgg gtatgtatcc acgaaactta ttaaacgttc
    11221 aattgaaaaa gaaattgaaa ttgacctgat tccgacactt cttggttatg agtacaatgt
    11281 tgattttgga agatatgacg aattgatcaa tacggatatc gttcacacga gtatccatga
    11341 atctactgtt aagggcatca gatctatttt aaaaaatggc caggaaatga aagcaataga
    11401 tcgaagaaaa gagatctaca atttttatga agtcgaagca agcctacgat atggcatcgg
    11461 aaaattgtgg ttagatggaa gtaatattcg tcaaaacaaa ccatacattt tttggaacag
    11521 tagtgattta gaacttccaa tgaaagataa ataattatgg agaatcgttt gaaaatgatt
    11581 cctctataca ggattgtgct tatagttggc atggagttaa aaaccttaaa gaaggagcag
    11641 ttattcaaag tgaaagaacg tcataatcag ttactatgta gtaaaacggg tacgatgaac
    11701 attgaatacc gaacagaata tttagaatta acgaatcaaa ctgatttttt gttgattcga
    11761 aagcaacaag agcaagaaaa aaattaatgg ctacttatag ccaattgatt gatgaatggg
    11821 aacaattcaa ggaatctaaa attccagaac gtctctaaaa gcctttattt gtaaagagca
    11881 taaggaaacg tgggaacact ttttaaaaaa agtgttccaa taggctgtgc ctattacgtt
    11941 tctcaaaaat aaatgtggga acaaaatatt taaaaatagg atagtgaaag gatagtgaag
    12001 gatagtaaaa ggagggtagt aggatagcaa aaaggatagg ctccttttcg ctatcctttt
    12061 taaattggag gaattaatat ggaagtaaga ataagaaatg tggatgagaa aatcgtaaga
    12121 gaaattgatc gaagatgtga cgaacggttc tgttgcaaag ttttaaatct actatcaaat
    12181 aaggtagaat aatagaaaaa gatagcagga ggaatgacga tgaatc
//
LOCUS       DANKHL010000083        12191 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000083 DANKHL010000000
VERSION     DANKHL010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.086"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            1..369
                     /locus_tag="PW101_001587"
                     /pseudo
     CDS             1..369
                     /locus_tag="PW101_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(495..1574)
                     /locus_tag="PW101_001588"
     CDS             complement(495..1574)
                     /locus_tag="PW101_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2513988.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(1677..2117)
                     /locus_tag="PW101_001589"
     CDS             complement(1677..2117)
                     /locus_tag="PW101_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2513989.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2174..2584)
                     /locus_tag="PW101_001590"
     CDS             complement(2174..2584)
                     /locus_tag="PW101_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL2513990.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(2764..3198)
                     /locus_tag="PW101_001591"
     CDS             complement(2764..3198)
                     /locus_tag="PW101_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2513991.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(3298..4146)
                     /locus_tag="PW101_001592"
     CDS             complement(3298..4146)
                     /locus_tag="PW101_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2513992.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            4535..6301
                     /locus_tag="PW101_001593"
     CDS             4535..6301
                     /locus_tag="PW101_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDL2513993.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            6403..7662
                     /locus_tag="PW101_001594"
     CDS             6403..7662
                     /locus_tag="PW101_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2513994.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            7809..9110
                     /locus_tag="PW101_001595"
     CDS             7809..9110
                     /locus_tag="PW101_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2513995.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            9113..9964
                     /locus_tag="PW101_001596"
     CDS             9113..9964
                     /locus_tag="PW101_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2513996.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            9977..10792
                     /locus_tag="PW101_001597"
     CDS             9977..10792
                     /locus_tag="PW101_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HDL2513997.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            10943..12109
                     /locus_tag="PW101_001598"
     CDS             10943..12109
                     /locus_tag="PW101_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2513998.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         4120 a         2166 c         2279 g         3626 t
ORIGIN      
        1 tccagaagaa aaaccagatg tgactccaga accagatacg gacgcaagag accaaggaat
       61 accagaaaaa ataaacaaaa aaacaattca agaggatgga aaaaaagaga gtaaaaaatc
      121 aaatctagca attctaaaaa taaatgaaga gcagctgaat aaaaaatcac gaatatttga
      181 tagcgcccaa tcagctgaaa ccttaaaaag tagcaaggat acaacctttg ctgtcctgaa
      241 acgaaaaata aacagctacc aaaatccgga gagtcacaaa acaaagttat actgtggagc
      301 ggaatcattc tattaagtat agcaacaatg ctttctgcaa aaagatttaa acaaaacaga
      361 tctctctaac atttgaataa tgtctgtgaa cgcatttttc aaataaaaag aaatatccag
      421 aaaatgaccc caaaatacca gattgaaatc tgatatttgg ggtcgttcat actttatctt
      481 tcttcatatt tattttaagg gatatctgtt tcttttggaa gatggttcgg gatctttttc
      541 actttcaata catctaaaaa gaaggtaaag atcggaatgc cgacaatcaa gccccaaacg
      601 ccaaagaacc gttcgctaac aagtaagacc acaaatgtat aaaaaatcgg cagttctgtt
      661 ttgctggaca tcaacttcgg attcagcaca taagactcaa ttagatggac aatcaaaatc
      721 gtaatcagga tataaacaac atctcggatg cctccttggg aataagctat gaaagataga
      781 ggaatacaag aaatgataac acctgctact gggatcagac ttaaaatgaa aatcatgatc
      841 gctaaactca gcagctgatg aaagccaaat acagccaagg cgattgtagt gagcaacgta
      901 tttaaaagag cgacaacaaa ttgagcttct aataccaaac caaatgtatt gacaaactta
      961 tcggcaaaat agtaaatatc ttgaaagaac caagaaaatt cactttttaa aaacaatctt
     1021 gagaatacaa ttgttttttt cttttcaatc ataaaaaaga aacttaagat gaatgacata
     1081 gcaaacgcca ctgccatttt tcctatatcc tgtaaatagc caagcgcaat cgaagcgcca
     1141 ttttgaagct gttcaaaaaa atcgtttcgc tcaatataag tatggatata ttgtaaaaca
     1201 ggatctgcat cttttggctg atgctcgtaa aaattaataa cggactggat catgtcaaat
     1261 gtttgatgaa taagcatcgg gatatacttt gtgaatgcca gatagataag gtaaacgatc
     1321 agtgtataaa tcatgatagc taaaacacct gtagggattc ggacaaatcg ctgaatgatt
     1381 tgaacaagat gaatcatcaa ataagtaaag ataaatgtga gcaaaatggt tgtaatcata
     1441 cttctaacta agaaaagaac aaaaacgaca aataataaaa cagtgagacg gcgtattttt
     1501 tcattcgcca aaaaacgctg ataaacggtc aaatcgacaa cctccaaata acaaaaaata
     1561 aacacgttat tcattatatg atggaatatg aaaataagaa agagcaaaaa gaaaaaccgc
     1621 taaatgacgt tatcttatga gttggttttc ctcataaaat cgttcatcta gcagtttcag
     1681 ccctttttct ggtcttgatg aaaattatct agcatttttc gggtcagatg ttgcagtgta
     1741 tccaattctt cctgttcaag tggaagataa gacttgatct tttgtggaat accttctgct
     1801 tgtttttgca acgcagcacc ttgagcagtc agcgtaacat ttacgattct ttcatcaccc
     1861 gtttcacgct gacgattaat ataatttttt tctgctaact tttttagtaa gggagtcagc
     1921 gtaccagaat ccagacacaa atcatttcct agctctttca ctgaagtctt tttcttttcc
     1981 cataaaacta acatcacaag atactgggta taggtaagat tcagcggctt gaggagggga
     2041 gtgtagtgtc taatgatttc tttagaggcg gcatataaag gaaaacatac ttgattaact
     2101 aaatatagat cttccatttt tccacctccg ctgttttact atagtgtatc atagcggatt
     2161 tacacaaaaa agtctattca ctaactgctt tcagagtaac ttctatattt ccagctgtag
     2221 cttttgaata cggacacact ttatgcgctg cctctaataa ttcttgcgtt tcttccagag
     2281 aaagaccttc gatagatcct tcaatctcta cacctaagac aaagctcgtt tcactttctg
     2341 aataaaggga aacttgtgca ctgacagtag aagccccaga gatatttttt tgctgtttga
     2401 tcaattctaa tgcactgtta aaacatgcac tgaaaccagc tgcaaacaat tgttcagggt
     2461 tggttgcatt ttcgacgcgt ttaccaggag ctgcaatatt caattcgaat gtacgatcgg
     2521 gattatgcac ttctccagag cgtccgcctg tgttgatcat tttcgtagaa tacatttttt
     2581 tcataaaatt accaccttta attatatttt atacaattga attttacaca atataattgt
     2641 ggaaagcaaa gaaatggatt ggcataagat aaataagaga aagcacatga aacagacgcg
     2701 ctttctcgaa tagcaaacat atacaaaaaa tatcagagac aaaaaataat ctcatcataa
     2761 acattacata tatttctcaa aaagttgttt tctttcttca aaaattactt cacgatggac
     2821 ctgatgaagc atccataaat aaccaaaagg atctctgaac attgagttag tcacaccaaa
     2881 gtccatcatt tctgtcattt cttgaatctc tacacagcca tttcgtaatg ctttattatg
     2941 tgtgtcctcg atatcagaga cgatgatatt gaaccagatc gtatttggat gttcagcagt
     3001 aggagcagtc aaaccaagag acgtgttttc atccagcata tggaatctcg taccatacaa
     3061 ggtaaaaatg acttcattat tgcctctagg gtaatctgtt gcctcaatca gttgtatttc
     3121 atcaaagatt gtttgataca gttctagtgc tttcaaacta tcatttaata caaaatcgat
     3181 ttccgttcct ttatgcataa agatctcctc tccttttcag tcgaacagtt actagattct
     3241 tttattatac cagctaaaga aaacgcattc aaaaaaatag cggatttact taccaactca
     3301 ttcgattgga tgataagtaa atccgcgtcc cattacttct gctacgctgc tgacaatgac
     3361 aaaggcactt ggatcgattt cttctatcaa tggtttcatt cgggaaaatt catgatcagt
     3421 cacaacgatc aataaaacct cttttgactt tcgattatac ccgccttttg cctctagcag
     3481 ggtaaggcca cgtccaatct cagcacttaa gcgaaggcgg atgtcttcca aataatgttc
     3541 actcatgacc ataacagatt tctttcgatt gatacctgtt tcgatataag tcatgacaat
     3601 tgaagtgatt gcaatagata aaatagcata aagaaactct tctattccaa aaataaacag
     3661 attaagagaa acaacgaatg cgtctgtaaa aaataagcca atcgaagtat tcaagtggaa
     3721 atatttttta aaaatgaggg gagggatcgt cgtaccgccg ctagaagagt tgtttttata
     3781 taaaatcgca actcctaaag caaaaatcgc acttccaaaa atgacagaca acaaccgatc
     3841 tgaagtaatc attgtttctg gaactattgc aatgattaaa ggaaaaacca agcttccgaa
     3901 aagtacttta aaaaagggtt ttttacctaa aaatagaaga gctagtacca acataacgat
     3961 attcaacact aaaataacta tagctcggtc aaagcctaaa gcagcttcca gtaatattcc
     4021 tagaccgctg acacctccag cagcaatatg atgaggccct aaaaacatat tgatagcgac
     4081 tcctaatatg aagagggcaa aaataatctt aatgtatgtc catataaatt gtctcgtttc
     4141 tttcatttat tcacttcctt aattcattaa taatactctt atcttattat aacttacttt
     4201 tcaccttaag ttaactttaa ttaataaaaa aattaggcta gtctaaatta ttatgtttca
     4261 ttcttctaga atatacttct acgccaaaaa ctgttaaaaa acttttactt tagaaaattg
     4321 gagaaattga tcgcttaaga taaattcagg acactaaata gtacaggaag tagactaaaa
     4381 cttttaaaag ttttaagtct atctatttgg caagtttaaa aaataaaaat tcggtatcaa
     4441 aaaactgtta ccgataacat ttttgaaagc gttgtctttt gcttctctat cacttatagt
     4501 gatgacgtca tattaaaaca caaggaggaa atcaatggat acagctgcaa tttatcatcg
     4561 accggaaagt gaatatgcat ttctatatac gaatacaaga tttcgtattc ggcttcgtac
     4621 taggaaaaat gatatcaaaa aagtatatgt gctatgtgga gatccatata ccataacaac
     4681 tgaaaaatgg tatcaaaaac aacgaccaat gaaaaaatgt ctaagtacca atgttcatga
     4741 ttattgggaa attgaagtga cgagcgaaac aagtcgctta cagtatgcgt tccatgttgt
     4801 tggggaagat aacacggact gtttttacgg tgatcaagga atttttcctt atcgagaaaa
     4861 tgtgattact gaaccaaatt tttattttcg aataccttat tttcatcaaa tcgatcgatt
     4921 cactattcca gaatgggtaa aagaaacagt atggtatcaa atttttcccg aaagatttgc
     4981 aaacggggac aaagcaaatg accccgaaca cactttacct tggggaagta aagatccaga
     5041 ccgagaagac tttttcgggg gagatttgca aggtgtgatg gatcatttgg attacttgac
     5101 ggaccttggc atcaatggca tctatttttg tcccatattt aaagccacat ccaatcataa
     5161 atacgatacg attgattatt atgaggtgga tcccgctttt ggcgataaaa aacttttaaa
     5221 aaacttgatt gatgaagcac ataaaagagg gatacgtatc atgttcgatg ctgtattcaa
     5281 tcatatgggt gtccattcac cacagtggca agacgttctc aaaaacggag aaaaatcgat
     5341 ctataaagac tggttccaca tccgtttttt cccagtagat tcttatcaga tgactgactt
     5401 accagaaact gctgaaaata tcccatatga tacttttgct tttactccat tcatgccgaa
     5461 actaaataca gcaaatccag aagtgcaaaa atatttgctg gatatcgcaa cttattggat
     5521 aaaagaattc gatattgatg gatggcgtct tgatgttgct aatgaagtcg atcatcattt
     5581 ttggaaaaaa ttcagagaag cagttacaga aatcaaacca gacatctata tccttggaga
     5641 gatttggcat tcctctcaag cttggctgca aggggatgaa ttccatgcag tgatgaatta
     5701 tgcatttaca gattcgatta aagattattt tgcaaaaaag aaaatcacag cttcacaaat
     5761 ggtcagtgga atgaatcatc agcaaatgct ttacagagat caggtgaacg aaggaacatt
     5821 caacttatta gattctcatg atactgccag gatcttgacc ctttgccaag gtaacaaaga
     5881 attgatgaag tctgtgatgg cctttatgtt tcttcaaaaa gggtctcctt gtatctatta
     5941 cgggacagaa atagggatga ctggtgaaga tgacccggat tgccgcaaat gtatgatatg
     6001 ggagaaggag gaacaagatc tagagctatt cggttttata aaagaacttg tttctctaag
     6061 gaaacagttg tctaagatta tttctgaagg ttctactcag tggctaatag taaatgatcg
     6121 tgaagataag ctctatttca cacggaaact agagggacag atcatctatg tttactttaa
     6181 tcagtcaaaa gaaccttggg tagtagaaca ggaaaatgag gtgattctta gtcaaaattg
     6241 tcagttgtta gaagacggga aagcagagat acgacaatac ggatttttga tcatgtgttg
     6301 aaaactgtta ccggtaacaa caagaaagcg tttgcaaatg aagatttgtg cccattataa
     6361 taagggtgta acagcacgtt gaatacaaag gaggaaatga agatgaaaaa aagaagtgtg
     6421 aaacagttgg gtttagggtt aatctcttta ggaatcttag gcggattagc ggcttgtggc
     6481 aatggtaact cttcagcaga aggggaagcc gatgaaggaa aaacgttaac agtttccgtt
     6541 gattctggat ataaagatta tataaacgaa ataaaaaaca ctttcgaaaa agaaaacgat
     6601 gtgaaaatca agctagtaga aaaggacatg ttcgatcagt tggaatcatt agcgttagat
     6661 gggcctgctg gaaagggacc tgacgttatg atggcagcat atgaccggat cggtgcgtta
     6721 ggacagcaag gacatctggc ggaagtaaaa ctgggaaatg aagcagctta cgatgaaaca
     6781 gacaaagccc aagtgactta tgatggaaaa atctacggag aacctgcagt aatcgagacc
     6841 cttgttttgt actataacaa agatttagtc gatacagcac catcaacgtt taaagatttg
     6901 gagaatctat ccaaagacag tcgttttgct tttgagtcag aagcaggaaa aaatacagga
     6961 tttttagcta aatggactga cttttattat tcctatggtt taattgcagg ttacggtggc
     7021 tatgtatttg gtgatgatgg cacaaatcct tcagatatcg gtttgaataa tgcaggtgct
     7081 gtagaaggaa tctcttatgc aacagattgg ttcaaaaatg tttggccaaa gggaatgcaa
     7141 gacataaaaa gtgcaggaga cttcgccagc cagcaattca tgtccaataa aactgcagca
     7201 atcattgacg gtccatggca agcgcaaaca tacaaagaaa acaatatcaa ttatggtgta
     7261 gccaaaattc ctactctaaa taatggacag ccttatcaac cgtttggcgg aggaaaagga
     7321 tgggtagtca gcaattatgc taaaaacaaa gacctctctc aaaaatggct tgattatgta
     7381 acgaatcagg aaaatcaaga aaaattcttt gaaatggtaa atgaaatccc tgcaaaccaa
     7441 caagctaggg aaacagcaaa ggctaaaaac gatgaattaa caacagctgt gattgaacaa
     7501 tatgaaacag ctcaagcgat gccaaacatc ccagaaatgg gcgaagtttg gacaggtgca
     7561 gaaaacttga tgttcgatgc agcatcaggt tcaaaaacac caaaagaatc cgcagatgaa
     7621 gcagtaaaaa caatcagtga agccatagaa caaaaatact gattaaaatg ataaccgtcc
     7681 atttttataa aaaatggaac aggtgcttca gataaaagtg gcaagagcga gatggaaaat
     7741 tatcttgctc ttgttgccat tatcttaaaa gcagtttgcg gtttagcaag tttagagggg
     7801 gcattcagat gcagcaagat aaaacaagca atatcaacga aaaaaaaatc acgctttgtt
     7861 cacttattcc agggatgggg cagtttgtaa atggccaagc ttttaaaggt atcgtttttt
     7921 tcgccatatt cgtcctattc attggtcaga tgcttctggg cggatttcat gcattggtag
     7981 gattggttac tttgggaagt ataccaatgg aagatcattc attatttatt ttgattgaag
     8041 gcactttgca attcatcatt acaatcattt tcttgatttt ttacggtttg aatcttttag
     8101 acgcttatcg agtagcgaag atgcgtcaag agggcaaaaa aatcaatcgg acgatgaagg
     8161 aagtcataca taatataggt gatgaaggtt ttccttatct attgacactt cccgcttatc
     8221 ttctcatgat ttttacgatc attttcccag tcctagttac attgttcact gcatttacta
     8281 attatgactt caatcatatt ccaccagcca gtttaattga ttgggtaggg gctaaaaact
     8341 tttttagtat cttcttttta agctcatatc gtaatacttt ttttgcagtc ttttcgtgga
     8401 ccgtgatttg gactttatgt gcaacaactt tgcaaatcac ccttggtatt ctcacagcgg
     8461 ttgttgctca ccagaaattt attaaattca aacgtatttt cggtatcatt ttcttattac
     8521 catgggcggt cccagctttt attactatca tgagtttttc gaatatattc aacgatagcg
     8581 taggtgccat caatactcaa gtgattcctt tcataaacca gcttcctttt gtagatatca
     8641 gtgcaatctc ttggaaaacg gacccgaact ggacgaaagt agcaattatc ttagtgcagg
     8701 gctggttagg atttccctat atctatgtga tggttacagg aatcttacaa tcgatttcag
     8761 aagacttata tgaagcagca aaaatcgacg gagcaaatgc atggcagcgc ttttcaaaca
     8821 tcacgcttcc tgctgtcttc ctagtggcag cgccaacatt tattacacag tatacgggga
     8881 atttcaataa tttctccatg atctatctgt ttaacaatgg cggtcccgga agcgtaggcg
     8941 gaggagctgg ttctactgat attcttattt cctggatcta taaacttacg actggaactt
     9001 ctccgcagta ttctatggct tcagcaatca cgttgattat ctccgtcatc gttatctgtg
     9061 tgtcattgct ggtatttaaa aagacaaatg cgttcaatat gggggattaa caatgaaaaa
     9121 atttcattcg agtgctcgat tccaaaaacg actttctcac ttttttactt acttttttct
     9181 tttaatactg tcagtcatta tcatctatcc gcttttgatc acagcaagta ctgccttcaa
     9241 gtcaggaaat attgcagctt ttagtttaga tctttcaagt gactggactt taggaaactt
     9301 tactcggcta ttcagtgaaa cactttacgg gacttggtat aaaaatacat tgatcatttc
     9361 tgtcctaaca atgatcgtac aagtgactgt agtaactttg gcgggctata cttatagtcg
     9421 ctatcgtttt atcggccgca aatcaagttt gatgttcttt ttagtgattc agatggtacc
     9481 gactatggca gcattgactg ccttttatgt catggctttc ttattagaag cgctcgataa
     9541 gtactggttc ttgacaatga tctatatagg cggtggaatt ccaatgaata cttggctgat
     9601 gaaaggatac tttgacactg taccaataga tttagatgaa tctgctaaac ttgatggggc
     9661 agggcatttc aggatttttt tccagattgt attaccgctt gtcaaaccga tgctcgccgt
     9721 tcaagcatta tgggctttta tgggaccttt tggggatttc atgttagcta agttcctgct
     9781 tcgaacacct gaaaatcaga ctgttgctgt cggcttacaa acatttattt ctgatgtgac
     9841 aaaccaaaga gtctctcttt ttgctgctgg ggcaattttg attgcactac cgatttgtct
     9901 attattcttc tttttacaaa agaactttgt taccggcctg acatctggcg gaacaaaagg
     9961 ataggagcag agtatcatgc aaacatttcc gttaaactat ttttccgcat tacgtacgcc
    10021 aactcaagtg tttgcgggac gcaaactatt atcatggcct aaatttttct tgatatttgt
    10081 atttttagtt tccttgatgg tcatgcctgt gactcttttt tatgccaatc agatccaagc
    10141 gattccatta gaacaatttc tatccgttca ttcgctgatc gatgaacaag gaacccaaaa
    10201 attttctgaa cttgaattat ctgaaactgg gttacaagca tcgcaacaaa cgatagctgt
    10261 gacccctgaa attttggtag gtgtgtcttt atctgagaaa caacaatccg atcatggaac
    10321 ttttattgat tttgagaagg aacaatgggt gatccaacaa aaggataaat ctggtatacg
    10381 ccgctatacg atgaattata gcccatcttt ccaaccagat tctgtacgta cgcccgaaga
    10441 tttccaacga tttttagaaa gagaatttta tgccagtaat cgtccaacaa tcattttatc
    10501 ttatagttta agtcttggtc ttgtcctatt tgtcatgacg agtctcattt tattcggggc
    10561 ttcctttttc ttgtggatga caagaaaaag ccaactttca tccattcata cattcaaaga
    10621 gagtgcaaat ttgatgctga atgttatggg aataggttcc atggcagcag cagttgtagg
    10681 gtttatccac tttgacttta tcttgatgct aagtgtccaa acaacgataa ctgtcttgct
    10741 gcttttatgg gtttttgtca aaacaagatt caaagataag cgtgtcgaat gacaagttta
    10801 agcgatttat gcgacaatga atgtgaaaaa aatgaaaaat aaagtagttt ttgaaaaatt
    10861 tctgaaaaaa ttaaacttct tagttgattc ttttcttttt tagtattaac gtaatagggt
    10921 agttttttta aggaggaaaa tcatgttaga gaaaacttta tacaatcaat tattttcacg
    10981 ttctttctcg ctccctgtcg aagtaaatta ttgggacggt acaacgaaac aatacggaga
    11041 tacggacact cctccccaaa tcaaaatcaa tatccatgaa gaaatcccaa tgaaagagat
    11101 tacaagtaac gcttctttgg cactaggaga agcctatatg gatcatcgaa tcgagatcga
    11161 gggaagtatc caagcattga tcaacgatgc ttatcagcaa gcaaacagct ttttacgagg
    11221 aaaagattat ctaaaatggc tgccaaagaa agaaaaacat acgaaacaaa aaaacaaaga
    11281 agatatccat gcgcattatg acttaggtaa cgacttttat aaactttggc tagatccaac
    11341 tttgacttat tcttgtgctt atttcaatac acctgaagat acgttggaac aagcacaaat
    11401 caataaagtc catcatatct tagacaaact ctttatcaaa gaaggcgaaa cgttgcttga
    11461 tatcggttgt ggctggggaa cattgatgtt cactgctacc aaagagtata atgtcaaggc
    11521 aactgggatc acactaagtg aagaacaata cgatttgatt accaaaagaa taaaagaaga
    11581 acatttagaa gataaatgcc gtgtattatt aatggattac cgcgaactaa aaggcgaaac
    11641 gtttgatcat atcacaagtg taggtatgtt cgaacatgta ggttctgaaa atcttgaagg
    11701 ttatttcaaa gtggtaaaag atttattgaa accaaaagga acagctttga tccatggaat
    11761 cagccgacaa caaggcggtg cgacaaatgc ttggatcaat aaatatattt tccctggcgg
    11821 ttatgtccct ggtgttactg aattagttga taatattaca aaaaacgatt tgcaactgat
    11881 tgatttggaa agcttacgtc gtgactacca attgacatta gaacattgga caaagaattt
    11941 tcatcaagtc caagatcaag tcacgaaaga aatgggtgat tccttctatc gaatgtggga
    12001 tctatattta caagcttgtg cagcttcatt tgaatcaagt aatatcgatg tgattcaata
    12061 tttgcttgtt caacctggaa acaatgacat cccaatgcat cgtgcataat tgaggcaaaa
    12121 gtactgttta aagactattt aaaaagaagc taagttttat gatgctccag aagtgaactt
    12181 ttttgtctaa c
//
LOCUS       DANKHL010000084        12128 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000084 DANKHL010000000
VERSION     DANKHL010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.087"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(90..254)
                     /locus_tag="PW101_001599"
     CDS             complement(90..254)
                     /locus_tag="PW101_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2513999.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            549..1877
                     /locus_tag="PW101_001600"
     CDS             549..1877
                     /locus_tag="PW101_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HDL2514000.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2079..2912
                     /locus_tag="PW101_001601"
     CDS             2079..2912
                     /locus_tag="PW101_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDL2514001.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2928..3665
                     /locus_tag="PW101_001602"
     CDS             2928..3665
                     /locus_tag="PW101_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HDL2514002.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3820..4788)
                     /locus_tag="PW101_001603"
     CDS             complement(3820..4788)
                     /locus_tag="PW101_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDL2514003.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5020..5853
                     /locus_tag="PW101_001604"
     CDS             5020..5853
                     /locus_tag="PW101_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDL2514004.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5887..6726
                     /locus_tag="PW101_001605"
     CDS             5887..6726
                     /locus_tag="PW101_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2514005.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6752..7312
                     /locus_tag="PW101_001606"
     CDS             6752..7312
                     /locus_tag="PW101_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2514006.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7511..9232
                     /locus_tag="PW101_001607"
     CDS             7511..9232
                     /locus_tag="PW101_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDL2514007.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9397..10539
                     /locus_tag="PW101_001608"
     CDS             9397..10539
                     /locus_tag="PW101_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2514008.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10555..11766
                     /gene="thiI"
                     /locus_tag="PW101_001609"
     CDS             10555..11766
                     /gene="thiI"
                     /locus_tag="PW101_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDL2514009.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4130 a         2035 c         2520 g         3443 t
ORIGIN      
        1 ttttctttta ttattatgat gaatcaagct ttctgttttt gtcacgtatg ttgtaatctt
       61 tacgaaagag tagagttggc tactcataat caaccatact tcccatcatc gctaaatacg
      121 aaataaatca gcattcctac accaatcaaa gcacaagctg caccaagaaa agggatgaca
      181 ttcccaaagt tatctgtatt cataagtatg ccaccaccca caaacaaaaa gccgccagca
      241 atcaggtttt tcattttatt catctccttt cttacgttat agtatttcct tctttcaatg
      301 agtattttga aagctatctt attgtatgat gttcttctag gcctcttctt tattatacag
      361 gccattttcg ttatctcata gaaatattcc tttttatctg aaaactattg tataccgtat
      421 gacatttttc ttcatgaagt tgtaagtata ttctcattca aatgaaaatt tcatcataga
      481 agagattgta tttctaaatt cttaagagta aaatacgaag tataacttgg atttggaagg
      541 atgattggat gaaagctatt acgcctgata tgacaacaaa atttaacaaa cagtttcaag
      601 caaatatcaa acagcaggca ttgcagcggg cagttgtgaa gaacggaatt actgcttctg
      661 ctgaaaatgt ttcagcacat gtggagaata caccggtatt ttctatagat ttggctacgg
      721 ggaaagtggc taatcaaatg caatcggggc gttgctggat gtttgctgca ctgaacacat
      781 tccgtcataa gatgctgaat caattccatt taaaagagtt tgaattatca caaaattaca
      841 cttttttctg ggataaatat gaaaaggcaa attatttcta tgaaaacatc attgcaacag
      901 gaaaagaggc aatcgatagc cggaaagtag ctttcttact cgctactcct caacaagatg
      961 gcggccaatg ggatatgatc gtttcattat tccaaaaata tggcgttgtg cctaaatcgg
     1021 tgatgccgga aagtgcgaat agttcaaatt ctcgagattt gaataactac ttgaacaaat
     1081 tactgcgtaa agatgcagta gtgttacgga aaatggttgc tcaaggagaa acattagacg
     1141 ctattgaaga aaagaaagaa gaaatgctag aaagcattta taacttctta gctatctcat
     1201 taggaacacc tccaaaagaa ttcgattttg agtaccgcga tgaagaaaag aactatcatc
     1261 ttgaccgagg actgacacca caaatttttt atgataaata catcggtgta aaattagatg
     1321 attatgttag cgtgatcaat gcaccaacaa aagacaaacc attcaatcgt tcttatacag
     1381 tagagatgct aggaaatgtc gttggcggga aagaagtcaa atatctgaat gttgatatgg
     1441 aaactttcaa aaaattagcg attgctcagc ttgaagaagg agaatctgtt tggtttggtt
     1501 gtgatgtagg tcaatcttct actagaacta gtggcatcat ggccttagat gcttattcaa
     1561 tggatgatct atttgatacg gattttacaa tgactaaagc tgaacgttta gattttggag
     1621 aaagtctgat gacccatgcg atggtattga ccggtgttga cttagtagac gggcaatcaa
     1681 caaaatggaa agtcgaaaat agctggggcg aaaaagttgg aaagaatggc ttctttgtta
     1741 tgagtgatgc ctggatggac gaatatacgt accaaatcgt tgtaagaaaa gatttattga
     1801 cgaaagagca attaaatgcg ttcaatgaag aaccaattgt tctatctccg tgggatccaa
     1861 tgggtgcttt agcatagacg agtatttcaa tgaagagtat gtagtaaaga taaaaagagc
     1921 tggcttaagc cagctctttt tatctttaaa gtccattccc tgttttttat ggtccctgtg
     1981 ctataattct aaataaagga taaggattgg aaataatcct tttatcatta aaaatatgat
     2041 gacgaagtag aataaaattg ggagtagggg ggaaagtcat ggatatccaa actgttttat
     2101 ctgatctgaa attaaatggt aaagtgattc cagttgttgg tggcgatgtt aatcagacct
     2161 atcgtataaa aaccgaacat cgtgcttatt ttttaaaaat ccatccgaat gtaaaaaaag
     2221 gattttttga agcagaagta gacggattaa aagaactgtc agcattcgta cgtgtaccgg
     2281 atacgtatat gctaggtgaa acaagtgaag gcgcttattt gttaatggaa tggatcgagc
     2341 cgggaaaagg ggatcaacga gatcttgctg cagctttagc taatctccat cagcaaacag
     2401 cgccacaatt tggttttcgt aaagataact atttgggtac acttgttcag aaaaattctt
     2461 ttgaagaaga ttggtggact tttttcttta aagatcggct tgaatcccag atttctttgg
     2521 cagaagaaac gaatcgatgg aatgtacagc gtcaagaaaa gtatctgcga tttaaagaac
     2581 gtgtattgaa atcggttgaa ccaaaaaaga tcactccacg cctcttgcac ggagacctgt
     2641 ggagtggtaa tgtgttcttt gaccagcaag ggcatccagt ttttgtcgac ccagctgttt
     2701 cttatgggaa tcgggaacaa gatattgcaa tgagccagtt attcggtggt ttccgaccgg
     2761 aatttttaga tgcttatcaa actatatttc cattagaaaa gggatggaaa gaccgactgc
     2821 cgatttacca gttatattat cttttggcac atttgaatat gtttggagaa agttacggtt
     2881 cccaagtaga tcaattacta gaaaattttt aagaggaggg cgtgtttttg gcaaaaaaat
     2941 ataccaaaaa gcatgaagtt tcatattatg agtgtgacat caaccaaacg atgacatttc
     3001 cttcgatgtt aggagtagtt atcaaaacgt ctgaagatca aagcgaccag ttgggaagag
     3061 gcagtgaatt cgtcaatagc tttggactta cttgggtcat cacgaattat tccatggaga
     3121 ttaatcgtct ccctagagtc ggtgaagtaa tctcagtaac gactcaagcc atggagtaca
     3181 ataagttttt ctgttatcgg aacttctgga tccatgatga agatgggaaa gaattagtca
     3241 aaatagaatc tgttttcgta ttgatggatt ttgtgaatcg aaaaatgagc agtgttaatg
     3301 aagagatcat tgctcctttt gagagtgaga agatcaagaa aatcaagcgt caggaaaaaa
     3361 ttgaaaagat tgattcgggc ttcatgttgc catatcgtgt tcgtttttat gatatcgata
     3421 gtaatcagca tgttaataat gcgatgtatt ttaattggat cattgacgta ttaggatatg
     3481 actttttgac tacgcatgtt ccagaaaaag taattattcg atttgataaa gaagtagagt
     3541 acgggaatga gatcgaaagc cattttgaac aaatccctat cgaaaatggt gtcaaaacaa
     3601 aacatgaaat ccgaatgcaa gaacagctgt attgtgaagc aaatattata tggaaaaata
     3661 aataaaaaag aggttgtgac agaagggggc aacttcaaga aataagaaga aatttacgaa
     3721 aattgtttct caaatttttg taaattttcg acttagtttt cgaagaagct atttttgtca
     3781 cagcctctct ttttaagttt agctattaga taatgatgtt tagttttgga taaacgaaat
     3841 caattcttca cccgacaaac catagtttac agcaatcaat agttttaaac gtgctttttg
     3901 actattaata ctgttacaga aaataatccc cattttttct agttcttttc ccccgccttc
     3961 ataatcataa acaggctctg cgattccatt gaaacaacga gaaacaagta ctacagggat
     4021 tttttggaca agaagctttt ccaaagcagc taaggcttgt ggagggagat tccccgcacc
     4081 taaagcttcg attaccaaac cgtctacttt cgtatgggcg atggcttcca ataagtcccc
     4141 ttgcatccca gcatatgctt taacgatcgg gatagtccca tctacagcgg aaatgtccag
     4201 tcgctttgtt tctaaaagtt cctgcaagaa taaaattcga ttcttagtta ctaagccgat
     4261 gggacccaat gtaggtgttc ggaaagtagc aacatttgtg gtatgggtct ttgtcacgta
     4321 gcgtgcagaa tggatctcat cgttcatgac caccaaaacc cccttatcta gcgcttcttc
     4381 acaacttgcg acacgaatcg cactttcaaa attataaagg ccgtcacttc caagttcatt
     4441 acttgaacgc attgccccag tcaaaacaat tggtaatttg ttgccaatcg ttgtatcaag
     4501 aaaaaaagca gtttcttcta atgtatctgt accgtgtgtg atcaccactc cggatatacc
     4561 tgatgcataa gcggacaaga tacgttcttt taataccaac atgtgagaag gcatgatgtg
     4621 tggtgaggga agattgaaga tatcttcaac taataattct acatgcttgg ggattgtgat
     4681 ttctgcatct aataatgggt ttgccacatc aggactaaca ccgcccgtga gcatgttttc
     4741 tttcatggcg atcgttccgc cggtgtgtag tacaagtatt tttttcattt tacagacttc
     4801 cttacgaata attttgcata gccgttaaat cttattgtat actatataaa agagttttgc
     4861 tatcagtgaa gaataacgaa tagcctaact tatcataaaa aagctgacct tattaaaatg
     4921 gaaaagactc aaccttgacc tcctaataca atggagtatc tggttgagtt tcttttacaa
     4981 gaaagcagcc aaggaaatca atcacgaagg tggccggaaa tgatcagaat cggattaaca
     5041 tcgtttaaag aacatgacaa attaactggt aaaaagaata acactcttta tgaatatgca
     5101 gggtttctac ccttagtgga attagatact gcctattatg gaattccccg aaagtcctca
     5161 gttgagaatt ggataagtga agtacctgaa tcatttcgct ttgtagtcaa agtctatcaa
     5221 ggaatcagcg gtcaaggaga ctggaagcct tattatcaga ccgaagaaga gatgattgcg
     5281 cattttttag atgttatgtc gccaatggta gatgctggaa agatgttttg ctttttagtc
     5341 caattcccct catcattcaa atgtacgaaa gaaaatgtcc agtacttgcg caaacttaga
     5401 aagtggtttg agggttatcc tgtttctata gaattgcggg attattcttg gtacagcgat
     5461 gaatttcttg aacgtacaag aaactttatg tctagagccg ctttttcttt agccatcatc
     5521 gatgagccac agttgctgaa tacaacgatt ccttttgatc catttgtcac caatccgaac
     5581 tttgctcttt ttcgattcca tggtaggaat gcgcaaggat ggcaagcaaa tgataaagag
     5641 tggcgtaaaa aaagaacgtt atatcgttat tcagaaaaag aactgcaaga actagcacat
     5701 gaagtcaaag tagttgattc taagacaaaa gaggtaggtg tgatttttaa taataattct
     5761 ggtggtgatg cggctgaaaa tgcattagca ctgcaaaaaa tattaaatat caagccagat
     5821 ggactcaatc caacccaaat ggatctgttt taagggctta ttttattcaa gaaaggggcg
     5881 aagtctatga tcaaagctat tttttttgat attgacggaa cattggtcac caatcgttca
     5941 aaagcgttgg aatcaacaaa acaagcaatt gaatatgctc ggaaaaacgg cattttatgc
     6001 ggtgtagcaa cggggcgcag cccagtaaag atcaaagaaa tcattgatga attggaatta
     6061 gatatgtatg tcgtgtataa tggtcaactg gtttttactg cagatcgcac gattatcgac
     6121 cacccttttg aacaaaaagt attggaacat attgttgaat ttgcagatga gaaccatcga
     6181 caaatcgttt ttggtgcgag aaatcggtta gatggatcaa cgacgatgct attaggacaa
     6241 tcgattttta tcaagcgact agtcagcttt ttaccgcgta aattcccggt acgtctgatg
     6301 aaaaaaatcc tccaggtttt tagccctcac cgacaaaagg atcgttatga aacgttagag
     6361 atattaaaag aaccaattta tcaatgtata ttgctcagtc cggagtcaga acagaaaaag
     6421 ttagaacatc gctttcccga gtgtacgttt cagcgttcaa atacgtatac tgtagatatc
     6481 atcccaaaag gtggttcaaa attactaggc atccaagctt tcgcaaatgc tgtcgaaatt
     6541 gaaatggaag aaattatggc attcggcgat cattacaatg atatcgagat gctgaaaggt
     6601 gtaggaattg gagtggccat ggggaatgct cagatcgaag taaaacatgc tgcagacttt
     6661 gtgactcaat caaatgaaga agatgggata tacgaaggac tgaaacactt cggtctgatt
     6721 tattagtcaa tgaaagaagg aaaaaatcag tatggaagca gagacacctt ttcaaaaagt
     6781 agaaacattc cacaaaactt ttgatccaag aagaccagag atacccacgg cttttacaga
     6841 aaatgaagct catacacgtg ctgcgttcaa aatagaagaa cttgttgaat ttgttgcagc
     6901 tacttcacct aataaagaac aactgcatga agctgttatg ctgctgcacg aagcattaga
     6961 taaagcagaa gaaaaaatac ttggtaaaac acagtgggga agtgatccgc tggttgaaca
     7021 agtagatgca ctgacagata tcctttactt tacttatggt tctttttcat tactaggaat
     7081 agtgcctgat cgaatattcg aaatcgtcca tcaggcaaat atggggaaat tatttccaga
     7141 tggaaaacct cattatgacc ctgtaacaaa taaagtgttg aaacctgaaa actggcagat
     7201 ggaatatgct ccagaaccta aaatcgcggc agaaatccaa cgtcagattc aagaagcgat
     7261 tgctaagaag aaatccgctg atataggcat gtgcacaaat gaaaaagagt gatcagagtt
     7321 tcactatttt tgttatcttt atacgatgga aaaacaaaag attttcgttt aagttagtga
     7381 atgcatgaaa atagtatgct ataatctaga atagatgtct tttgcacgag agacgtatta
     7441 tttggattta tgccaagcag aacgccatct gttgcataaa agatgcatac tgaaaacgga
     7501 ggaaccataa atgggaaata atgttatcat cggaatcgtc attgcaatca ttatagtcgc
     7561 cattgtcgtc tttttggtta gttacttgat gaagaaaaaa aaccaagatc gattggatgc
     7621 cttagaaaaa cgcaaggaag aattatttga cttgccagtg attgaagaag tcgatgatgt
     7681 taaaaaaatg catctggttg ggcaaagcca aaataccttt cgtgaatgga gtcaaaaatg
     7741 gaatgacata tctacttcct cgttcgctga actagaaagt caaattttcg aagtggaaaa
     7801 cctcaatgaa agttttcggt tcttaaaagg aaaaaaagca gttgttgaag cagaagctac
     7861 aatggaccaa atggagaaac aagtgacgga aatccgtgaa ggtctaaagg aattgcggga
     7921 aacggaagaa cgcaactcac tagctgttca gcaggcactt gacgtctatg aagaactgaa
     7981 agaaaaagtc agagaagacg agcatgaata cggaccagct ctttttgaat tgcaaaaaca
     8041 gatcaaagtg atcgaaaaag aatttacgca gtttgtgact ttaaacactt ctggagaccc
     8101 tgttgaagcc cgcgaggttt tggataaagc agaaaaacat acgtatgaag tggaagctct
     8161 gatgaaaaaa attccaccgc tgtatgagga gctccatacc actttccctg aacaacttga
     8221 agaaatcagt gatacttacg agaaattgat ggaagaacaa tatgtattcc cagaagaaga
     8281 tcttgctgaa gatatcgcaa aagtaagtcg aagaattgaa aattcattag ccaatcttga
     8341 aaaaacagaa gttgaaacgg tcgaatttga aaaccgtgaa acagctgatt tgattgattc
     8401 gttatatgat atcttagaac gggaaatgga agctcagaga tatgtaaaaa ctaaccaatc
     8461 aacgattgca gagtatatca aacatacaac aaagaataac cgtcaattat tgattgagct
     8521 ggatcacaca tctcaaagct atacattgaa ccacaatgaa attggccgtg tacgaggttt
     8581 ccaaaccgaa gtggaagaaa tggaacgtca aaatgaacag atgattccac agatccgtca
     8641 acacgaaatt ccttattcag aagtgcgcac gttctataaa acagtcttca aagtcttaga
     8701 agatatcgaa aagcaacaag tagagatcga cgactcatta catgaattga gaaaaggcga
     8761 aaaagaagcg caagaaaaga tcgatacatt cgaattcaaa ttgcgcagtt tgaaacgttt
     8821 tgtagaaaaa caacgtttgc ctggcttgcc gaatgattac ctagaattct tttttgttgc
     8881 tactgaccgt attgaagaac taagtgttgt cttgaataaa attcgagtaa acatggaaga
     8941 ggtcaatcgt ttagttgctc tatgtgaaga agatctagaa ttattagata agaagacaca
     9001 tgatttagta gatgcagcag cattaacgga gcaaatgctc caatatgcaa atcgttatcg
     9061 tcatacacac gaagaagtga gagaagcaat tgataaaagc tactatttat tcaataaaga
     9121 atatcactat caagaagcat tagacgaaat cggtacagca ttagagcgag tagaacctgg
     9181 tgctttcaaa cgcatcgaag atttttattt taatcatcca gacttagtat aaagcaaaaa
     9241 agttagtaaa ccagcaagag caagacggat tattgtcttg ttcttgtctt tttttgtata
     9301 tttgaaaatt gaaacaagtg aaaatttcac ctgctcttga aagcaccttg gacaaccgtt
     9361 ataatagaaa agaatttcaa aaaagagggg tttcttatga tatattttga taacagtgca
     9421 acaaccccaa tctttccgca aagcttagat gcatatgtaa aaaccagtca gcggattatt
     9481 gggaatccat caagcttgca tgatttaggc aatcaagcta accggttgtt gcagcaagca
     9541 agaaagcaga tcgctgattt gatccatgta gaaccagaag aaatctattt tacttcaggc
     9601 ggaacagaag gagataactg ggtattaaaa ggaacgatga tcgaaaaaag agaatatggg
     9661 aatcatatga tcatttcagg agtcgaacat ccggccgtca gcgagacagc agaacaatta
     9721 aaagaattag gaatcgaagt aagtatcgcc ccagtggata aacgcggatt tgtacgtgtc
     9781 gacgaattga aagaattaat aagaaaagag acagtattag tctctgttat ggctgtgaat
     9841 aatgaagtag gatcgattca gcccatccag gaaatcagtg atcttttgga tgattttcct
     9901 aaaattcatt tccatgtaga tgctgttcag gcgattggaa aagtaccatt ctcgcaatgg
     9961 ttgacagaaa gagttgattt tgcaacattt tcagctcata aattccatgg cccacgggga
    10021 actggtttca tttattggaa aaagggtcgt cgtttagcac cattactgac aggtggaggg
    10081 caagaaagaa atcagcgaag tgggacagaa aatgttccag gtattgtggc tatggttaaa
    10141 gctttacgtt tatcattaga gataaaggaa caaaaacctg agcagacaca acgcttaaaa
    10201 gtagctctaa tcaatgcgtt gaagacgtat gagaaagtga cgattttttc agaaggaact
    10261 gactatgcac cgcatatttt atgttttgct ctaaaaggaa ttcgcggcga agttctcgtc
    10321 catgccttag aagaaaaaca aatatttgtt tctactacta gtgcctgctc ctcacgtaaa
    10381 catatggcaa gcagtacatt gcacgcaatg catgtaccaa atgatctggc tacatctgct
    10441 gttcgaatca gcttagatga aaacaattcg cttgcagaag tcgagcaatt tatgattatt
    10501 ttcaagcagc tttataagaa attttctgca atcaattaaa agaggagtgt tcttgtgaaa
    10561 tacagtgaaa ttatggtccg ttatggggaa ctttcgacaa aaggaaagaa tcgaaaaagc
    10621 tttatcatgc aactagcaca aaatgtcaga caagctttga aagactttcc agaaatcaaa
    10681 atccatgctg atcgggaccg gatgcatctt ttattgaatg gcgcagacgg gcagctagtt
    10741 attccaaaac tagcaaaaat atttgggatc caaaatttct ctccaagtat tcgtgtagaa
    10801 aaagatgtcg aagttttgaa acaagctgtt caagacatca tgaaagaaat ttatactggg
    10861 aaagaaacat ttaaaattat cgctaaaaga agcgatcacc aatttgaatt gacaagtaat
    10921 gaattgaatc aaacattagg taatgctgtt tttgatattt tcccgcatat ccaagttcaa
    10981 atgcgtcaac cagatattcc attacgtgta gagattcggc gtgatggcgc ttatctatcg
    11041 tatgaaacga tcaagggagc tggaggattg cccgtaggaa caagcggaaa aggaatgctt
    11101 atgctttctg gggggattga ttctcctgta gcagggtact ttgccatgaa gcggggagtg
    11161 gaaattgaag ctgttcattt tgctagtccg ccgtatacaa gtgaacaagc actgcaaaaa
    11221 gcaaaagatc taacagccaa actagcacct tatgtgggaa cgatccattt tatcgaagtg
    11281 ccatttaccg agattcaaga agaaatcaaa aagtcagtgc cacaaggata ttggatgaca
    11341 attactcgtc gaatgatgtt gcgtttgacc gatgaaattc gccgtattcg tcatggttta
    11401 gtcattttga atggtgaatc attaggtcaa gtggcttcac aaactttgca aagtatggtg
    11461 gctatcaatg aagtaacgaa tacgccaatc attcggcctg tagcgacaat ggataaactt
    11521 gaaatcattg aagtagcaga acagatcgat acatttaatc tttctatcca gccttttgaa
    11581 gattgctgta cgatttttgc tcctccacag ccaaaaacac gccctcaact ggaaaaggtc
    11641 catcaatatg aagagcgttt agcaatagat gaaatgatcc aacgagcact agctggcttg
    11701 aagatggaag agatacaagc agaatcgtct aatgaaaaaa ctgaagaatt ttcagaattg
    11761 ctataagagg ttatgaaaat agtgttttga ccagagtatt agagaaacaa aacgaaaaaa
    11821 tagctccatg tattttaccg tttttgaact aatgtcgaag attagctgat tgaataccat
    11881 ttatcaagaa gttatggaaa agccggttct tttttactaa ctgaaaaata aaattttatt
    11941 tagagatcaa gctacagttg ataaatgata taaaaaagag aatttcccat gaaatagcga
    12001 ggttgtgaca gaagtgggca gcttcaagaa ataagaagga atttacgaaa attgcttttc
    12061 aaatttttgt gaaatttcgg cttatttttc gaagaagtta cttctggaac accgtgtatc
    12121 agaggttg
//
LOCUS       DANKHL010000085        12036 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000085 DANKHL010000000
VERSION     DANKHL010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.088"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            30..2048
                     /locus_tag="PW101_001610"
     CDS             30..2048
                     /locus_tag="PW101_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2514010.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    2118..2324
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2475..4889
                     /locus_tag="PW101_001611"
     CDS             2475..4889
                     /locus_tag="PW101_001611"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDL2514011.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            5237..6790
                     /locus_tag="PW101_001612"
     CDS             5237..6790
                     /locus_tag="PW101_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL2514012.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7171..8955)
                     /locus_tag="PW101_001613"
     CDS             complement(7171..8955)
                     /locus_tag="PW101_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2514013.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(8970..10691)
                     /locus_tag="PW101_001614"
     CDS             complement(8970..10691)
                     /locus_tag="PW101_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2514014.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            10791..11552
                     /locus_tag="PW101_001615"
     CDS             10791..11552
                     /locus_tag="PW101_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2514015.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         3941 a         2178 c         2437 g         3480 t
ORIGIN      
        1 atagataaat tgaagttgga ggatagaaga tggaaaacat tgcaagaaaa gaacggagac
       61 gtttagaaca gaccaaacgt tttcgaaaag caaaacgagg ggcagcaatc gtgggaactg
      121 cgatggtagg ctgttctgtc gctgccccat tagtacagcc ggttcctgtt caagcggatg
      181 aaacacctgt tcagttcggc gcaagaatca atacagcggc atttatcaca gaaattgcta
      241 cttatgctca accaatcgct caagccaacg atctatatgc atcagtgatg atcgcacaag
      301 cagttgtaga aagcggttgg ggcagtagtg cgttatctca agcaccttat tataatcttt
      361 ttgggataaa aggcagctat caaggacaaa ccgtctacat ggatacgctt gaatttttaa
      421 ataatcaatg ggtaaataaa aaggagcctt tccgacaata tccttctttt gctgaatctt
      481 tcaatgacaa tgcctatgta cttagaaata cttcatttgg caacggttat tactatgccg
      541 gtacttggaa gagcaacacg aaatcctata cagatgcgac agcttgtttg acagggcgtt
      601 atgcgactga tcctggttac gcaggcaaat taaataatat catcgcaact tacggattga
      661 cccaatatga tacaccagct agcggaaacg caggcggtgg agcgactaca ggtggcaata
      721 atactggaag tgcaggtagt actggcggtt caagcaattc tggtaataca aactcaggtt
      781 cagcaacttc gaccacttat actgtccaat ctggtgattc tgtttgggga atctcaaata
      841 aattcggtat cacaatggat caattgatcc aatggaacaa catccaaaac aatttcattt
      901 atccaggaca aaaactgaca atcaaaggta gccaggaaaa tggctcttcg acgaataatt
      961 ccggtaacaa taccaactct tcaggaaatg ctggtacatc aaacggagga caaacaactg
     1021 gtgcgaaata cactgttcaa tctggtgatt ctgtttggaa gatctcaaat gatcacggaa
     1081 ttacgatggc tcaattgatt gaatggaaca atatcaagaa caatttcgtt tatccaggac
     1141 aacagctaat cgtatcaaat ggcggttctt ctaatgctgg agcaaatact ggctcaaata
     1201 ctgggaatac aagtggctct agttcgtcca gctcaagttc ttctaataca gcttcaggaa
     1261 cgaaatatac agttaaagcc ggagactctg tttggagcgt ctcaaataaa tacggaatta
     1321 cgatggctca gctaatccaa tggaacaata tccaaaataa ctttatctac ccaggacaac
     1381 aactgactgt ttcaaacagt ggatcaacaa acagcacgtc aggtaataca ggttcgtcaa
     1441 ccaatacaaa taacagcagt tcagttaata ctggcagcag ttcgtctcaa ggcacttata
     1501 ccgtgaaagc aggtgaatct gtttggagcg tggcaaataa aaacggcatc acgatggatc
     1561 agttaatcga atggaataat atcaaaaaca acttcattta tccaggacag caattgatcg
     1621 ttaaaggtgg aacttcggca aatacaaata caggttcaac tacttctgca gcgaaaccaa
     1681 atacaccgaa tacgtctgct acgacaagta catcatccac tggtaatacg atgtataccg
     1741 tgaaagcggg cgagtcagtt tggagcgtag cgaacaaaca tcatatcacg atggatcagt
     1801 taatcgaatg gaataatatc aaaaataatt tcatctatcc aggacagcaa gtgattgtga
     1861 agaaaggttc atctcaatca acaaaccaac aatcagttac tggttcgaaa acctatactg
     1921 tgaaatctgg agaatctgtt tggagtgttg ctgacagtca tggaattaca atggctcaac
     1981 taatcgaatg gaacaatatt aagaacaact ttatttatcc aggtcaaacg ttgattgtag
     2041 ctaaataaaa tagctgttcg aagaaataac gattgaattc cacaaaaata tttgttacta
     2101 taaggctact taaatatgac aacacttgaa aaggaagtag taaaaagact gaagtttcag
     2161 agagcgcatg gctggtgaga atgtgcacgg atgcttttga actcgccttt gagttgccct
     2221 tgctgaatac ccgagtaagc aagcggcggt gacggccgtt acaacgttcg aggccttttc
     2281 actaaggtaa aattaggtgg taccgcgtag attacgtcct ataaaaagag caatcttttt
     2341 ataggatttt tttatttctt ttgtgaagtt tgagaaaaca aagcaaaagg aaataaaaat
     2401 aaacagattc tgccggtttt ttcgttatat ttataataga aacaagtgtt ttcaatagaa
     2461 caggaggaca gcaaatgagt tacaatcata aagaaattga gaaaaaatgg caaaaatact
     2521 gggcaaaaac gaatactttc aacacccatg atgatcctga aaagccaaaa ttttacgcac
     2581 tggatatgtt tccctatcca tccggtcaag ggctacatgt agggcatcct gaaggttata
     2641 cggcaacgga tatcctttca cgtgtgaaaa gaagtcaagg atacaacgtg ctgcatccaa
     2701 tggggtggga tgcattcggt ctgcctgctg aacaatatgc gctagataca ggaaacgatc
     2761 cagctgagtt tacccaaaaa aatatcgaaa cattccgacg ccaaatcaat tctttaggat
     2821 ttagttatga ctggaatcgc gaagtgaata caactgatcc ggagtattat aaatggacac
     2881 aatggatctt tacgaagctt tatgaaaaag gtctagctta tgaagcagaa gtagcggtaa
     2941 actgggtacc tgaattagga acagttattt caaatgaaga agtgatcgat gggaaaagtg
     3001 aacgtggtgg ctatgatgta gtcagaaaac caatgcgcca atggatgctg aaaatcactg
     3061 cttatgctga tcgcttattg gatgatctag aattagttga ttggccagaa agcattaaag
     3121 aaatgcaaag aaattggatc ggtcgttcag taggagcaaa tattgaattt aaagtggctg
     3181 gtacggataa atcctatacc gtatttacga cacgaccaga tactctattc ggtgcaactt
     3241 actcggttct tgcacctgaa ctagatcttg ttagagaaat tacgacccct gaacaaaaag
     3301 ctgctgtaga agcttatatc gaagagacag ctaaaaaatc agatctaaag agaacggatc
     3361 ttgcaaaaga aaaaacagga gtatttactg gtgcttatgc aatcaatcca gttaatggca
     3421 aggaaatccc aatctggatc gccgattatg tattatcttc atacggcaca ggagccatca
     3481 tggctgttcc agcacatgat gaacgtgact ttgaattcgc tcaaactttc ggactagaaa
     3541 ttcttccagt tatcgaaggt ggagatgttg aaaaagctgc ttacacagaa gatggtacgc
     3601 acatcaactc tgaatttcta gacggcatga acaaacaaga agcaatcgat aaaatgaatg
     3661 aatggctaga agaaaatggc gtagggaaaa aagaagtcag ctatcgctta cgtgactggc
     3721 tattctcccg tcaacgttac tggggtgaac caatcccaat catccattgg gaggacggaa
     3781 cagtcacagc tgtcccagaa gaagagctac cattacggtt gcctaagaca aaaaacatca
     3841 agccaagcgg tactggagaa tctccattag caaatatcga ggagtgggtc aatgtcgttg
     3901 atcctgtaac aggtaaaaaa ggccgtcgcg aaacgaatac aatgccacaa tgggcaggaa
     3961 gctcatggta ttacctgcgt tatatcgacc ctcataacaa aaaagaacta gcagattatg
     4021 aaaagttgaa gcgttggcta ccagttgaca tctacattgg tggagcagaa catgcggtac
     4081 ttcatttact atatgcacga ttctggcata aattcttata cgatatcggt gttgttccta
     4141 caaaagaacc attccaaaaa ctatacaatc aaggaatgat cttaggcgaa aacaacgaaa
     4201 aaatgtcaaa atctcgtggt aatgtagtca atcctgatga tgtggtcgaa acttacggtg
     4261 cagatacact gcgcatgtac gaaatgttca tggggccatt agatgcttcg attgcttgga
     4321 gcgaaaatgg cttagaagga agccgtaaat tcttggatcg tgtatggcgt ttgatcgttg
     4381 atgagaacaa taaaatgcgt gaccgtatca ctacattgaa tgatggcaag cttgataaag
     4441 tgtatcatca aacagtgaaa aaagtgacag aagactatga aaatcttcat ttcaatacag
     4501 caatctccca attgatggtc tttatcaatg aagcctataa agtggacgct ctgccttatg
     4561 aatatatcga aggatttgtt caactactcg caccgattgc tcctcacatg ggtgaagaac
     4621 tatgggcaat ccttggaaat gatggtggta tttcttacgc accatggcca acatacgatg
     4681 aagcagcact tgttgaagac gaagtagaag ttgttttcca agtaaacggt aaagttcgtg
     4741 cgaaaagcaa tgttccccga gacttaggaa aagatgagct agaaaaagca gcattagcca
     4801 atgaaatagt gcaagaatat attgaaggca aaacagtcag aaaagttatt gcggtaccaa
     4861 acaagttagt caatatcgtt gctaattaat tggctctttg tcaataagga ctgatgagtt
     4921 gtgcaaaatc aaatctgggc cagaatgaac ttcattctgt ctcagatttt ttgttatttc
     4981 aaattgatta attgattgat caaaaaggtt ttgattttca tgttctcaaa cacgttcaat
     5041 gtctttttct tctgaatggt cttgtaagag atctctgtaa tcatcaaatt tttgtctatt
     5101 aatcttagta ttttttgatg tataaactac ctgaaattga gagtacatac caaattcata
     5161 aattttttat tagtgggact agtgattgta tggattttga cgcataatga atgaagtatg
     5221 agaaaaggag agacaaatgg atcaagaact atttattatc gaagtattat cagctaaaca
     5281 aaaaattatc gagaaagcga aaaaaagagc gaaaagaata atgttcaagc ttttaagctt
     5341 agtatttttc tctggtttgc tttttttcgg agctgtatcg ttcctatatc gccatatgag
     5401 tgcagtagtc cgttcagctg aagggatgca aaaattaaat aatttgtatc acttttcgtg
     5461 gggagttggc ggaataacaa ttgttgtgct tattgttttt cttctactta tttggctccg
     5521 ttaccatcaa ttgtcgcgtc atttcgatca atggcttagt caaagagcac aacagttttt
     5581 acataataaa ccaacttctc aagatgatga ctatctttat tttgagaaga cttcgacgag
     5641 tcaagctttt cggatacaaa aaagtgattg ccagttattg ctgagatcac ttgatgattt
     5701 acctatctac ttaggcgaaa gcaaatcaaa attcggaatg aacacattcc atattttttt
     5761 actagaaaac gaacccagat gggaaaaaag ccagtcttcg cctggtggat acctgacaac
     5821 taagatgagt acattagtca ctggtttttt cctcattctt ttagctggaa caatctatta
     5881 tgggagatac actcagccaa aaatgtcttc tcatataaat gctgcttctc acatgacaat
     5941 gaactcaact aacgatacgc atatacaaaa gacaaatgaa gggttgttag ctgatcctaa
     6001 gcaaaaggta aatttgacaa cacaaaagat aaatgatctc aaacttgata cagagacgca
     6061 ctctctctat atgacaacag atagtggtgc taattgggta tttgtgccga ttgattcgca
     6121 atggttgcgt tttggcgaat acaccctgac aacggggctg attcctgaag gttattggat
     6181 ggacaacaca tatgatattt ccgccgattt ctcatggttt atctattctt ctggggatga
     6241 tgtacgtctg ctgacttcaa gagataatgg taaaacatgg caaacgtcta ttatcaccca
     6301 atcagttcag ccgatacgtt atcgaaaagc ggtattttat ggcaacgatg gtctatttat
     6361 ctgtacatca gccagtggga tgtctgcaga aactttgtac atttacacta ctatggatgg
     6421 cggaatcact tggataaaaa gtggaagtac aatgatcaat cagccagtac aaaatgtcag
     6481 cttcgttaca acttctttag gttttgtttc gacacgtgaa agtttgtttt atacaaacaa
     6541 cggaggacgc tcattcaaag aatcagttct gtcgattccg gaaaactatc agacaggggg
     6601 gatagatctt tttcaatcac caaatgaagt tactcaaata agcgcgaata cgttagagac
     6661 aaaattttat ttgttgaaaa aaggaccaat cgatgcaggg aaaatgtttg cctgcctctt
     6721 ccgttcaact gataacgggg aaacatggca attcgttcag caactatcgc aagtagaaca
     6781 aggagattaa agaagagaga ttgtgagcct gacgttcagc accgagtcac aagaaacaaa
     6841 ttcaaaaaac agcttctcat atttgttgaa tagtgtaact cgtgattcgg agttgacaag
     6901 agcaacaaga agcgattttg aagtatagct tctcgtattt tacaagatcg tgtcttattg
     6961 ccgataggtt gtctcttgaa caccgtttat tcgtaaaaag ggattgtgac aagtcttttg
     7021 tcactactta ccgaacgaaa ctgaaggagc tatccaaaaa gtatcattag tattattctt
     7081 tgtgtttcta gtggcaccac tagtacattt aaaaaagcta tcgatcatgg actaatcata
     7141 catgatcgat agctttttag ataatgtttg ttacgctatc tgaaattgac tattataaag
     7201 actggcataa agcgttggtg attggagtag ttcagaatgt gttccttttt cgacgatatc
     7261 gccattcttc attacaagga tcatgtctgc attttcaatc gttgacaaac ggtgtgcaat
     7321 cacaaaactt gtcctatctt ttgtcacagc ttccattgct tgctgaatca atgcttctgt
     7381 tcgtgtatct acgctagaag ttgcttcatc taaaataacg acaggaggat tcgctagaat
     7441 gactcgagca attgtcaata attgttgctg tccttgagat aacgtgccgt tttcgctaga
     7501 aatgatcgta tcatatccgt ctggcagtgt tcgaataaag tgatcacatt gtgcgatttc
     7561 agccgcttcg ataatttgtt ctcttgtggc atccattttc ccgtaggcga tattatcagc
     7621 taccgttcct tcaaacagcc aggtattttg taaaaccatc ccaaaagaac gacgtagttc
     7681 tccacgtgaa agcttggtaa tatcaagacc gtcgaattcg atctttcctt tattcagttc
     7741 ataaaaacgc atcagcagat tgactaaagt cgtttttcct gcaccagttg gaccaacgat
     7801 cgctaccgtt tgttttggct ttactgaaaa atcgacttga ttcatcaata ttttatctga
     7861 tgtgtaacca aactgtacat ttttgaaagc tatttttcct ttcggttgtt ctaaaagtct
     7921 tgcatctgga aggtctggaa tttcatctgt ttcatctaat atctcaaata tcctatcgat
     7981 tgctgccatt gcggattgga tcgagttgat cacataagaa gcggtagaaa ttggttcaga
     8041 aatctgattg atgtactgaa gataagcttg taagaagccg atcgtcacac cacctgatag
     8101 aacaagcata gcaccgaata ccgcacttat gatgaaggcg agttgattaa tgaaacgaat
     8161 ggccggatag atagcacagt tcataaattg agcacgttta aaagcgttgt attgcttttt
     8221 atttacttcg tgtatagcag cttctgcatg agcttgctga ttgaatgttt tgaccactag
     8281 attaccagct aagatttctt ccattcgatt atttaaaaca cccagttcat actgattctg
     8341 ttcagaaatc tgtttgtttt tgttcgcaat cttagttgta acaattgaac tgccaacgat
     8401 cagcaataag acgagcaaag taagcttgct ttcgatataa aacagcatca gaccagcagc
     8461 agctattgtt acgacagaag taaaaaattg attgatacct gtcaataaca cttgagaaag
     8521 ttgattgaga ccggtagttg tccgactaat gatgtcacct acttgatgat tgtcaaaaaa
     8581 cgacatcggt aatgttttga atttttttgt gatttctttt cgcaccctca atgtgatctt
     8641 ttcgcttaac gctgccattg tccgttcctg aataaatgaa gtgatgctgc ttaagactga
     8701 acagccgatc agcaataaga cagggaacaa caatgcttgt ttgatatcac tgaaagaaag
     8761 ctgtccaata cctgtttttc ttatttctga taagagatca tcgatcccga ttcccatgat
     8821 caagggcatg gctacgacta ataggttccc gaataaactg catagaaaca atccgtaaaa
     8881 atatttatgt tcaggtttga tcattttcca aaaacgtatc aaagcacgcg tgttcaacgt
     8941 ttttttcttt tccatcgtat cccttctttc tatattcctt gtgattttgc aaactcctga
     9001 taagtttggc aattagacag tagttcttca tgagtacctt gtccaacgat ttttccttca
     9061 tctaaaacaa tgatgttatc tgcttgttgg attgtactga ggcgttgagc aacaatcacg
     9121 agagttttct ccgacatgtg tttatgaagg gctgctctta atgctgcatc tgttttatag
     9181 tctaaagcgg agaaactatc atcgaataca tagatctctg ctggtttgat caacgctcga
     9241 gcaatacata tccgctgttt ctggccccct gaataattag aaccgccttg agcaacaaag
     9301 ctgtccaatc cgtcaggaag ggtgtccact agttcttcta attgagcgat cttgatcgct
     9361 ttagacaact cttctgttga ggcatattca tttcccatct gcaagttgct taaaattgtc
     9421 ccgctgaata agaaagcctt ctgagggaca taactgatgt gggagcggat ctgttcttga
     9481 gtcatttgag taagaggaat accaccaagg gttacttttc ctatcgtagg gtcactaagt
     9541 ctcaagagaa gtttcgccaa actacttttt ccagatccgg tcccgccaac gatcgctgtt
     9601 gtttttcctt taggtattgt gaaatcaata tcttctagaa ctggatcaga gatttcagta
     9661 tatttaaatg tgacatgttc aaaagcagct gcatttgtct gatcaaaggt tggttcagct
     9721 actgttttga tcgtctcttc ctcaatttcg atctctgctt gcagtatttc ttctatccgt
     9781 cgtaatgagg caaacgattt agggataatg accaatacca tagctgcgat catcaagtaa
     9841 gcaagtgtca gcatagagta ttcgactaca gcagtcacga caccgatttg taatgctcct
     9901 tctgctacaa gatttccgcc aaaccacaaa atagaagcat aaacgattcc catgagcaaa
     9961 aaagcaattg gagtgaccca agcaaatatt cggttcactt tgatcatttg attagcatag
    10021 tcagcaaagc tcttattcgt tcgcttttct tcatatgatt gattatcaaa ggcacggatc
    10081 atattaatcc cagtaaagaa ttctctcaat gtgatggtga tccgatccat ttttggctgg
    10141 atactcaaag ataaaggtgc ccctcgtttc atcagtcgat aaacaatcca tacataaaaa
    10201 acaacagaga tcaatgtaac ccatgttagt aatagagaat aacgaaacat catgagaatg
    10261 gtgaaaacgc aaataaccgg tgctggtaag atcatttggc aaaataaaac aatcatttgc
    10321 tggacattat cgacatcatt cgtcattctt gtcaaaagag atccagaact aaaacgactg
    10381 atatccttca ttgacagctg ttggaattta cggaaatatt tctcccgcgt ttggaatcca
    10441 aacctagcgg aagccaatgc tgaaaagtaa ctagaggcaa ctgcagcgat tgttcctaga
    10501 aacgctacaa gcagcatttc aactccaatt ttttttataa ctgaggcttc gccacttgct
    10561 atacctgtgt cgatcagctt tgctactaat tttggtacac ctaatgtacc ggcgatttct
    10621 aaacagatca tcacaaaggt aaggagagcg agtcctttgt ttttctgtaa gaatgttttt
    10681 aagatagaca tattattcct ccttttttcc tgatactttt cgattctata ccttcaagta
    10741 acttgaaggt caatagaaaa aagaataaac tgaaaaagag gtgagaaaaa atgaaaaaaa
    10801 tgtatacgat tggccaagta tcggaactat ttgatttgcc aaaatcaacg atccgctatt
    10861 gggatgaaca aggattaatc cgttcaagta gacaagaaga gaatgattat cgattgtttg
    10921 atatcgatga tatttttatg ctttatgata tcgtttttta tagaaaatta gatataccga
    10981 tcaaacagat gaagaattta tatggaaaaa cattgacgga gttatatgag acacttgatg
    11041 aaacagaaag aagaatccat aaagaactgg tggtgatgaa acaaaaacaa aaagagataa
    11101 gggagcaaaa aaaacagttg aaattgatga tcgatacaaa cgaggaagaa tttcctgtag
    11161 aagagatccc ttttgactgt atgatctcaa cggagtttga agatattgtg gaaatcaaaa
    11221 aatttctacc taactattca agctttggga tgatgagtat gccagcttcc agttcacaga
    11281 cgatgtatgg attttttatc gaccctagtg aagtacattt atttactcaa gacgtgattt
    11341 gggagaaaaa agatacggct gtttatcgtc gattcttatt gaaatccgag atgaaccatg
    11401 cggaacgtaa taatatttta gaaatcagag aaagaatgta tgaaaaagga tggaaaacag
    11461 gagaagtgat cggccagtat ctattgacga atacggatga aaacaatatc agaactgaat
    11521 attatcatgc atggatagaa atgaaaaaat aggaaatatg tgtttaagat aactcgaaaa
    11581 tgatggttgt tttagttata aagaaaaatt ttctggttcg attacgggtt taatgtgggg
    11641 gattattagt cgtggataga tagaatttta gttttttaga ggaaatatgt ctttaaaaat
    11701 ggaatggttg caattcttag cgatatatac gatgtaattg tcctcttaaa aaagtatgga
    11761 aaaataattg tattgggtgt tatgtataat tcatggtgaa ctgagactaa tgagaagaaa
    11821 aattcatatt gttatgagtg agattgtatc agatgagaat gataaattaa ataggtgcat
    11881 aaaagcatta cacttaaaat acattgttgc ttacattatt acaatatgtt aataaggcaa
    11941 aaataaatag atggattgaa taaacatatt ttttaatcaa atagtcccta ctgtgacttt
    12001 cctcagctat cggtgatact accgatagca tttttt
//
LOCUS       DANKHL010000086        11836 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000086 DANKHL010000000
VERSION     DANKHL010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.089"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            24..266
                     /gene="yaaA"
                     /locus_tag="PW101_001616"
     CDS             24..266
                     /gene="yaaA"
                     /locus_tag="PW101_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDL2514016.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            253..1377
                     /gene="recF"
                     /locus_tag="PW101_001617"
     CDS             253..1377
                     /gene="recF"
                     /locus_tag="PW101_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDL2514017.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1374..3320
                     /gene="gyrB"
                     /locus_tag="PW101_001618"
     CDS             1374..3320
                     /gene="gyrB"
                     /locus_tag="PW101_001618"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDL2514018.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3340..5811
                     /gene="gyrA"
                     /locus_tag="PW101_001619"
     CDS             3340..5811
                     /gene="gyrA"
                     /locus_tag="PW101_001619"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDL2514019.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6047..6346
                     /locus_tag="PW101_001620"
     CDS             6047..6346
                     /locus_tag="PW101_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDL2514020.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6396..6908
                     /gene="ssb"
                     /locus_tag="PW101_001621"
     CDS             6396..6908
                     /gene="ssb"
                     /locus_tag="PW101_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL2514021.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6934..7170
                     /locus_tag="PW101_001622"
     CDS             6934..7170
                     /locus_tag="PW101_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDL2514022.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7313..9286
                     /locus_tag="PW101_001623"
     CDS             7313..9286
                     /locus_tag="PW101_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDL2514023.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9292..9744
                     /gene="rplI"
                     /locus_tag="PW101_001624"
     CDS             9292..9744
                     /gene="rplI"
                     /locus_tag="PW101_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDL2514024.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10018..11385
                     /gene="dnaB"
                     /locus_tag="PW101_001625"
     CDS             10018..11385
                     /gene="dnaB"
                     /locus_tag="PW101_001625"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL2514025.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         3957 a         2152 c         2629 g         3098 t
ORIGIN      
        1 ataatgaaaa tgagggatgg gcattgaaac aacaagtcat tctaaatacg gaattcatga
       61 cgttgggcca aatgttgaaa gaagtcacag ttatcggtag tggcggacaa gcaaaatggt
      121 atttggcaga aaacactgtg ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac
      181 tttatcccgg catgatggtt gaagttccag aagtaggaac tttttttatg gtaaaaaaag
      241 gaaacgacga taatgaggct gaataagctg tatttaaaaa attatcgaaa ttacgaagag
      301 ctgaacatcg aattttccaa gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa
      361 acgaatcttt tggaaagtat ctacgtatta gctatgacgc gaagtcatcg gacaagtagt
      421 gagcaggaat tgattggctg gactgacgat caggcgatga ttcaaggaga gatcacaaaa
      481 ggttcatcca ctcttccatt agaaatctta ttatctaaaa aaggccgaaa aaccaagatc
      541 aaccatatcg aacaaaaaaa gttaagtact tacgtgggac aaatgaatgt cattttattt
      601 gcaccagaag atctttcttt agtaaaaggc agcccgcaga tcaggcggaa gtttttagat
      661 atggaattag gccagatcaa tccaatctat ctttatgatc ttgtccaata ccaagcaatt
      721 ttgaaacaaa gaaaccagta tttaaagcaa ttgaatgaaa aaaaacaaac tgatttgctt
      781 tatttggata ttttgtccga acaactagca gcgtttggca gcaaagtatt gaaagcaaga
      841 gcacagttcg tcaagcgatt ggagttttgg gctaattctt tgcatcagca aatcacccat
      901 caaaaagagc agctggaaat cgagtatctc accgcagtcg attctttaga aacacatacg
      961 caagagcaaa tccaagaaca atttctcgca cttttgaacc aaaataaaaa gaaagacctt
     1021 tttcggggaa caacgacagt tggtcctcat cgtgacgatc tttccttttt tattaatcag
     1081 aaaaatgtgc agacttatgg atcgcaagga cagcaaagaa caactgcact aagtgtcaaa
     1141 ttagcagaaa tcgatttgat taaagaagag acgggggaat atccaatcct tttgttagat
     1201 gatgtgatga gcgagctgga tgatagtcgt caactccatc tgctagaaac aatcgaagga
     1261 aaagttcaaa cgttcttaac aacaacgaca cttgaccatg taaaaaataa aatgactgtt
     1321 gaacctgaga ttttttatgt tcaacaaggg aaactaaaag gagaagaaac tgaatgacag
     1381 aagaaagaag tttagtagaa cgcgctaaag agtatgatgc cagtcagatt caagtacttg
     1441 aaggacttga agccgttcgt aaacgacctg gcatgtatat cggttcgact agttctgagg
     1501 gtctgcatca ccttgtttgg gaaattgtcg ataattcgat cgatgaagta ttagcaggtt
     1561 ttgcaacgaa aatccatgtg attattgaaa aagacaacag tatcaccgtt attgacgacg
     1621 gtcgggggat acctgtagat atccaggcaa aaactggacg tcctgcagtt gaaacggtct
     1681 ttactgtttt acatgctgga ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac
     1741 ttcacggagt aggatcttct gttgttaacg cgctttctac acagcttgat gtcaaagtat
     1801 acaaagaagg aaatgtgtac tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa
     1861 agattatcga acaaacggat cgccatggaa caacggttca ctttacgcca gaccctgaaa
     1921 tttttacaga gacaactgag tttgattttt caaaattggc tacccggatc cgtgaacttg
     1981 ctttcttgaa ccgcgggatg cgtatatcga ttgaagacaa acgagaagaa gaaccagtca
     2041 tcaatgaata ccattatgac ggcgggatta aaagttatgt cgaatacttg aatgcaaaca
     2101 aaaccgtgat ttttccagaa cctgtatatt tggaaggtga acagcaagat atcgcagttg
     2161 aagtctctat gcaatataca gacggctacc attccaatat catgagtttt gccaacaata
     2221 tccatacgta tgaaggcgga acacatgaat caggattcaa gactgcttta actcgtgtga
     2281 taaatgatta tgcccgaaaa caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg
     2341 aagacgtgcg agaaggattg acagctgtta tttctatcaa gcatcctgac cctcaattcg
     2401 aaggacaaac aaaaacaaaa ttgggcaatt ccgaagtacg gacagtcact gatcggttat
     2461 ttagcgaaca tttcatgaaa ttcttgctgg aaaacccaag tgtgggccgt caaattgttg
     2521 aaaaaggatt actagcttcc agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga
     2581 gacgtaaagg tgctttagag attagtaatc tgccaggaaa attagcggac tgctctagca
     2641 acgatcctga aaaatgcgaa ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta
     2701 agcaaggtcg taatcgagag tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg
     2761 ttgaaaaagc aagcatggac aagatcttgg caaatgaaga gatccggtct ttatttactg
     2821 ctatgggtac agggtttgga gaagattttg atgtatcgaa agctcgctac cacaaattag
     2881 ttatcatgac tgatgccgat gtcgacggcg cgcatatccg tacattgctg ctgactcttt
     2941 tttatcgtta catgcgtcct attgttgaag ctggatacgt ctatattgct cagccacctt
     3001 tatacggggt aaaacaaggg aagaatatta cgtatgtaca accaggaaaa aatgcagaag
     3061 aagagttgaa acaagtcgtt gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt
     3121 ataaaggact tggagaaatg gatgatcatc aactatggga aacaactatg gacccaagca
     3181 atcggatgat ggcccgcgtc agtgtggacg atgctattgc agcagatcag atatttgaaa
     3241 tgttgatggg ggatcgagta gaaccccgtc gtgcattcat cgaggaaaat gcacactatg
     3301 tgaagaactt agatatttaa tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa
     3361 aacatccatg atgtcaatct aaccagcgaa atgaaagact ccttcatcga ttatgcgatg
     3421 agtgttattg tcgcacgtgc acttcctgat gtcagagatg gtctgaaacc cgttcatcgc
     3481 cgtattttat acggcatgaa cgaattaggt gtaacaccgg ataaagccca taaaaaatct
     3541 gccagaatcg tcggagatgt catgggtaaa taccatcctc atggagacta tgcgatctat
     3601 gagtcaatgg tacggatggc acagccattc agctaccgtt atatgctagt ggatggtcat
     3661 ggaaactttg gttctgttga cggtgacggt gccgccgcta tgcgttatac cgaagcacgg
     3721 atgagcaaaa tcgctacaga aatgctgcgt gatatcaata agaacactgt tgatttccaa
     3781 agcaactatg atgatacaga aaaagaacca gttgtactac ctgctcgctt cccgaatctt
     3841 ttagtgaacg gaacaacggg gatcgccgta ggtatggcca cgaatatccc acctcataac
     3901 ttgtctgagg tagtagcggc tatcgatttg ctgatggata atcctgatgt gacgaccaat
     3961 gaattgatgg aagtattacc tggaccagat tttccaacag gtggtttggt tatgggaaaa
     4021 tcaggaatcc gaagagcgta tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt
     4081 gagattactg agatgccaaa tggtaaagaa cggatcatcg tgacagaatt gccttatatg
     4141 gtcaataaag caaaattgat cgaacggatt tctgaactgc acagagacaa gcgtatcgaa
     4201 gggatcacag atttgcggga cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc
     4261 cgtcgtgacg ccagcgcatc tgttatcttg aataacttat ataaattgac atccttgcaa
     4321 aattcatttg gattcaatat gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg
     4381 aaacaaattc ttgaaaacta tatcgaacat cagcgcgaag tcatcacaag acggacagtc
     4441 tttgaaaaag aaaaagcaga agcaagggca cacattttag aaggtttgcg tattgctttg
     4501 gatcatatcg atgaaatcat tgcgattatc cgcgggtcta aatcagatga cgaagcaaaa
     4561 gcgacgatga tcgaacggtt cgatttatcc gatcgccagt ctcaagctat tttagatatg
     4621 cgtttacgcc gattgaccgg tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc
     4681 ttaaaactga ttgaagattt aactgacatt ctggcaagac cagaacgtgt tacagaaatc
     4741 atcaagaatg aattagcaga cttgaatcaa cgatttggtg ataaacgacg cacagaatta
     4801 ctagtcggtg aggtcttaag tctggaagat gaagatttaa tcgaagaaga agaaatcgtt
     4861 attacattga caaacaatgg ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa
     4921 cgaaggggtg gacgtggtgt ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat
     4981 ctggtttctt gttctaccca tgatacattg ttattcttta cgaataacgg aaaagtgtat
     5041 cgggcaaaag gatacgaaat tccagaatac gggcgcacag ccaaaggaat accaattatc
     5101 aatttactag gtatcgattc gagtgaaagc atccaggcaa tcattgcggt gacgggagaa
     5161 gcagaagaag gccactatct cttctttacg acgcgtcaag gtacagtaaa acgtacttct
     5221 gtcactgctt ttgctaacat ccgaagtaat ggtctgatcg caattggttt gaaagaagac
     5281 gatgagctcg tcaatgtgct actgacagat ggtcaatcta atatcattat tggaacacat
     5341 aacggctact ctgtcacttt caaagaagaa gcagtccgcg atatgggacg tacagcttcc
     5401 ggggttagag gaatacgcct gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag
     5461 gaagatcaag aagtattagt gatcacagaa aaaggttacg gaaaacggac aaaagtatcg
     5521 gaatatccag tgaaaggccg cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa
     5581 aatggtccac tagctggatt gaccactgtt tctggagatg aagacatcat gctgattaca
     5641 gataaaggtg tcattatccg tttcaacgta tcgactgttt ctcaaactgg acgttcaaca
     5701 ttaggcgtac gtttgatgaa gatggaagct gatacgaaag ttgtcacgat ggcagcagtc
     5761 gaaccagaag ctgcagaaca aacagttgaa gaacaaggat cagaagaata gccatcaaga
     5821 ctaattgtgt aagctagcaa gaatgaccga ttaaaaaatc tggttattcc tgctggcttt
     5881 tttgattagt tgaatttctc ttaaaatatt cgaaactgtt cattctgtat tgcttttgac
     5941 caaaatagag tgtataatca gagcttgtga gtagtctaat taattagata actctccttg
     6001 ctcttaggaa cctaagagcc gaaagtccac agggaggtga atatcaatgg aaaacacgaa
     6061 atatgaaatc atgtacatta ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat
     6121 cgaacgtttc gatgcaatct tgaaagataa cggagcagaa gttatcgaat caaaagattg
     6181 ggaaaaacgc cgtttcgctt atgaaatgaa cggttaccgt gaaggtatct atcacatcgt
     6241 taaagtatct tctccatcat cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat
     6301 caacgacgat atcattcgtc acatgattgt taaagaagaa gcttaattgt tgtttcatgt
     6361 gaaacaatag aaaacgatga gaggagatga ctattttgat taataatgtt gtattagttg
     6421 gacgtctgac gaaagatcca gatttgcgtt acacagcaag tggtacggca gtcgcaacat
     6481 ttactttggc tgtgaatcgt aattttacaa accaaagcgg caatcgcgag gctgatttta
     6541 tcaactgtgt gatttggcgc aaatcagcag aaactttagc aaactatgct cgtaaaggaa
     6601 cacttttagg tgttactgga cgtatccaaa ctcgttctta tgataatcag caaggacaac
     6661 gtgtatacgt gacagaagta gtagctgaca acttccaatt gctggaaagt cgttctgcat
     6721 ctgaaaatcg ccaacaaagc ggcggattcc aaagttctgg tcaatcagct ggcggatttg
     6781 gcggaaacaa taactctaat caaacatctc aatcatccaa cggtatgcct gatttcgacc
     6841 gtgatacttc tgacccattc ggctcaagtt caacgatcga catttcagac gatgatttac
     6901 cattctaaac gaatttgata ggagggaaat agaatggcac aacaaagaag aggcggacgt
     6961 aaacgtcgta aagtagacta cattgctgct aaccatattg aatatatcga ttataaagat
     7021 atcgaattac taaaaagatt tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact
     7081 ggtacaggcg ctaaaaacca acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg
     7141 ggcttgctac ctttcgttag cgaagactaa taaatcctag gaattgaatg actgatcctt
     7201 ttgatcagct attcaattcc tttttttctg taaaaataac ctctattcgt ttagtcgctc
     7261 gaatgacgaa agttatggta aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa
     7321 taaaaacaat catttatccc cgaaacctct cttccttatc ctgtttatat taatcgaagt
     7381 attggtagtc ttaattttac catttcgttt tgttagcgct tcggtcattc tattgatgaa
     7441 tttatggggt gttcgctatt ggttgaaaat gaaacgttgg cttattttaa ataaacgtga
     7501 aaaaatccag caggcttcga ctgtagctga aaccaatcta gcttatgtct cagaagtcat
     7561 gcctgtagga gtgattgctt atcagccatc aagccaaaaa atcgaatggc tcaatccatt
     7621 agcattgaaa atcaaagatc aaagtggatt aacagaggaa gaattgctag atactttttt
     7681 tactgcaaaa gaaaaaaaga aaaaccaagt ctatctagca gataaaacat ttcagtttga
     7741 tatggataca gtaaagggtg ttgtttattt taatgatatt acccaaaacc agttgttaaa
     7801 actccatcag acgaacagtc agccggtgat tggtatttta tctattgata actatgcaga
     7861 tgccattgat aaattggatg acaaagagat ttcttatttg aactctttcg tcactacact
     7921 agtttctgac tggatgaatg aataccatgt ctttttcaaa agaatcaacg cagaacggtt
     7981 tttcttcgta gcacagatga tcgatctgaa aaagatgatg gataaaaaat ttgatttgct
     8041 ggatcgcttt cgagcagaag ctgaaaaaca gagctcgcct atcaccctta gtatggggat
     8101 tgcttatggc tcaagaaccc tcgatgaaat cggtggagaa gctcagaata atttagatgt
     8161 tgctttagtc cgtggaggcg accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc
     8221 tgtttattat ggggggaaat ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc
     8281 gatgagtacc gctttgcgtc ggatttttga tgaaacaggc gatgtgttta tcatggggca
     8341 caagtttccg gatatggatg ctttaggctc tgcttttggt gtcagttgtc tggcacgctt
     8401 caaccagaaa aaagcttaca tcgttatcaa cgagaacgag atcattccag atgtagaacg
     8461 gtgtcttgaa gaaatccata agcatcctga attagaaatg caactaatct ctccagaacg
     8521 tgccatagag ctgaaaaaag aagaggacct cctcgtgatg gtggattacc ataaaccttc
     8581 tctatcgatc tctcagggcc tatatgagca atttgataag attgtgatca ttgatcacca
     8641 tcgtcgtggg gaagagtttc caagtaaacc tctgctgact tatatcgaat cttcagcttc
     8701 ttcagcttca gagctagttt ctgagctgat ccaataccaa agcagcaaaa caaatcgctt
     8761 agatcgtttt gaggccacat tattgcttgc agggatggtt gtagatacaa atagtttcaa
     8821 tgtccgtaca actgcacgaa cttttgacgt tgccagctat ttaaggtctt gtggagcaga
     8881 cgcatcctta gtacaatatt tattgagctc ggatttagcg acatacttgg agatcagcaa
     8941 tttgattgct ggaagcgact atgtgacttc tgatattgtt gtggcggcag ggacagacga
     9001 aaaagagtat aatagtgtta tagcagcaaa aacagcagat acattgcttt cgatgatcaa
     9061 tatcaatgca gctttcgtca ttacaaagcg gacagacggc ttgattggga tcagtgccag
     9121 aagtaccggc acgatcaacg tccaaatcat catggaatcc ttaggtggcg gaggacactt
     9181 cacaaacgca gctactcaac tggaaaatac gacagttgag gaaacaaaac agaagctatt
     9241 tgctgcaatc aaagaaaata tgaatcaaat atatgataag gagtaatcca aatgaaagta
     9301 atttttttac aagatgtaaa aggaaaaggt aaaaaagggg acatcaaaga agttccagca
     9361 ggttatgcac aaaattatct aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt
     9421 atcagtcagt tagcaggaca aaagaaagca caagaaaaac acgaagctga aatcttggaa
     9481 gaagctaaaa agttaaaaga attcttggaa aaagaagata ctgtagtcga attgaaggcg
     9541 aaagcagggg aagacagccg tttgtttggt tcgatccctt caaaacaaat tgctgaaggg
     9601 ttaaacaaac aatacaaaat caaattagat aaacgaaaaa tcgaattaga aaatccgatc
     9661 cgttcactag gctacacaaa cgtacctgtt aagcttcatc atgaggtgac ggctacgatc
     9721 aaagttcatg tcgtacaaga ataacaaatc ttgatagcga tgttcagctg gtaagtataa
     9781 ccagaaggaa aaaagacaaa ccgaaattta tctgagaaaa cagctgcagc aatgaaagca
     9841 aaagctgcaa atcgatttca gattgatttc ggtttctttt ttgggaacag caaaatagca
     9901 taaagaaaac gagtataata aaagagaact atttttttat tacactttcg gtacgaatga
     9961 tgacttttct ctcattttct tgtaaaatga agcaattgaa gacaaggagc gatacgtatg
    10021 aatgaagtgt ggcaggatcg aatcccaccg caaaatattg aagctgaaca agcggtttta
    10081 ggctctgttt ttcttgacgc agaaacgatc attgatgcca tggagtacat agagccaaaa
    10141 gacttttatc gtcgtggcca tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt
    10201 aatgaagcga ttgacgtggt tactgtcaaa gaccggctgg aacaagccaa tttgttagaa
    10261 gatgccggtg gtttaagtta tctctcagac ttggcattag cagttccgac agcagcaaat
    10321 atcgtctact atgcgaaaat cgttgaagaa aaatcattgc tgcgtacatt gatccagaca
    10381 gcaacaggaa tcgttacgaa aggattcgag caaggagaag aagttcaaac aattttagat
    10441 gatgccgaac gcagtatctt ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct
    10501 attgctgaag tactgaactc cacgattgaa aatatcgatc gactagctca aaacaatgaa
    10561 gaaatcacag gtctaccaac tggctatcaa ggtctggata aaatgacagc aggtttccag
    10621 cccgaagaac taatcattct tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg
    10681 aatattgctc aaaatgtagg aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa
    10741 atgggggcag aatctcttgt aaatcggatg ctttgtgcag aaggttcgat tgaggccagc
    10801 catttacgta caggtcagct ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg
    10861 agtctttctc gagcaaatat ttttatcgat gatacaccag ggatcaaaat ctctgaaatc
    10921 cgtgcgaaat gtcggaaact agcgcaagaa aaaggcaatc taggcttgat attgatcgat
    10981 tatttgcagt tgattgaagg taacggtaaa gagaaccgtc aacaagaagt atcagatatc
    11041 tctcgccaac tgaaaaaact ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag
    11101 ttatctcgtg gagtagaaca gcgacaagac aaacgaccag tgctaagtga tattcgtgaa
    11161 tccggatcaa ttgaacaaga tgcggacatc gtagccttct tgtatcgaga tgattattat
    11221 gaaagagaag gcggagatga cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa
    11281 gtcatcatcg agaagaaccg aagcggtgca cgaggaacag ttgaactact gtttatcaaa
    11341 gaatacaata agttttcgtc gctttcgcca agagaagaat tttaaagaag aggttgtgag
    11401 ccttgagcaa tcccgagagc tctcgtaaga agatgcaagg agtaaccgca ggaacgacta
    11461 gagtacttaa tttaaacaga tctccaaagg gattgtgaca aaagtcttgt cacagtccct
    11521 ttttccgaat aaacggtgtt caatcagctg acccttggta ttagctcagc aaacgtcaaa
    11581 atatgaggaa ctattttgac gtttgtttct ctaatactca ggtcaaaacg ctgatcttac
    11641 gacctctttt ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatca
    11701 tcaaacatga gaagcagttt tttgaaattg ctccttgtga ctcagagtta aacggcaggc
    11761 tcacaacctc tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca
    11821 aacgaaaaat atgaga
//
LOCUS       DANKHL010000087        11731 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000087 DANKHL010000000
VERSION     DANKHL010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.090"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(5..586)
                     /locus_tag="PW101_001626"
     CDS             complement(5..586)
                     /locus_tag="PW101_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2514026.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(769..1395)
                     /locus_tag="PW101_001627"
     CDS             complement(769..1395)
                     /locus_tag="PW101_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2514027.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1417..2748)
                     /locus_tag="PW101_001628"
     CDS             complement(1417..2748)
                     /locus_tag="PW101_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDL2514028.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2903..3304)
                     /locus_tag="PW101_001629"
     CDS             complement(2903..3304)
                     /locus_tag="PW101_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL2514029.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3390..3596)
                     /locus_tag="PW101_001630"
     CDS             complement(3390..3596)
                     /locus_tag="PW101_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514030.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3691..5193)
                     /locus_tag="PW101_001631"
     CDS             complement(3691..5193)
                     /locus_tag="PW101_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDL2514031.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5211..5666)
                     /gene="argR"
                     /locus_tag="PW101_001632"
     CDS             complement(5211..5666)
                     /gene="argR"
                     /locus_tag="PW101_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL2514032.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5764..6381)
                     /locus_tag="PW101_001633"
     CDS             complement(5764..6381)
                     /locus_tag="PW101_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDL2514033.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6602..7975
                     /locus_tag="PW101_001634"
     CDS             6602..7975
                     /locus_tag="PW101_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2514034.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8050..9162
                     /locus_tag="PW101_001635"
     CDS             8050..9162
                     /locus_tag="PW101_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDL2514035.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9234..10871)
                     /locus_tag="PW101_001636"
     CDS             complement(9234..10871)
                     /locus_tag="PW101_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDL2514036.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11039..11647)
                     /locus_tag="PW101_001637"
     CDS             complement(11039..11647)
                     /locus_tag="PW101_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2514037.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3087 a         2472 c         2125 g         4047 t
ORIGIN      
        1 attattaaag aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc
       61 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
      121 tcaacgcttt ggcttgacgg tctgcttctt catcggacag tttttcttca gaaagtgcca
      181 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
      241 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag
      301 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
      361 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
      421 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
      481 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
      541 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
      601 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
      661 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
      721 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
      781 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
      841 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
      901 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
      961 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     1021 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     1081 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     1141 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     1201 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     1261 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     1321 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     1381 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     1441 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     1501 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     1561 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     1621 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     1681 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     1741 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     1801 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     1861 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     1921 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     1981 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     2041 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     2101 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     2161 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     2221 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     2281 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     2341 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     2401 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     2461 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     2521 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     2581 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     2641 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     2701 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     2761 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     2821 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     2881 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     2941 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     3001 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     3061 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     3121 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     3181 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     3241 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     3301 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     3361 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     3421 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     3481 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     3541 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     3601 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     3661 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     3721 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     3781 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     3841 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     3901 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     3961 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     4021 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     4081 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     4141 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     4201 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     4261 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     4321 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     4381 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     4441 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     4501 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     4561 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     4621 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     4681 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     4741 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     4801 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     4861 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     4921 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     4981 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     5041 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     5101 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     5161 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     5221 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     5281 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     5341 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     5401 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     5461 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     5521 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     5581 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     5641 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     5701 ataagtaata tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg
     5761 ttgctaacct ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt
     5821 tcttggatcg ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt
     5881 tgcgatgaat gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg
     5941 taagatttgg gcacaaagac cttcttccat ctttttgaat actacttgtt ctttcaagtc
     6001 ttccggaagt ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc
     6061 aggctgtcgg atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa
     6121 atacttttct tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc
     6181 aggaatcagc caattttttt taggcgacat gcgtattgca tagctgactg catatagaca
     6241 ggctactctt cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa
     6301 tgatttttta ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt
     6361 acgccattca attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat
     6421 catacctctt tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag
     6481 aataagtgaa gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg
     6541 tttctatttt tataatagaa atcactctct ttttttacga aattttttag aggtgatgta
     6601 aatgacaaaa caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc
     6661 ttttgcgggc gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca
     6721 gtttgattta acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc
     6781 gatcattgtt ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt
     6841 agcttcaaat ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat
     6901 ggtcctattg tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat
     6961 gttccatatt atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg
     7021 tactttgaca acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc
     7081 acattttaca tggaatcata tcttcctgtt tcttgtgccc gttttgtttc tttctttgat
     7141 tatcggctgg tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat
     7201 aagcgtttta ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg
     7261 aacagtttcc agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta
     7321 tcgaagtaaa aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt
     7381 ccgcttgttt ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt
     7441 cttgccgaat ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat
     7501 gcttcccggc gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca
     7561 atatggtccg aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc
     7621 attattccta ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta
     7681 tatgatcggt atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa
     7741 tgaagaacta caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc
     7801 tgtggctact tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa
     7861 ctatgaagtg tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct
     7921 tctcgtaagc tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc
     7981 atatacggca ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga
     8041 ggctttttta tggaaaaaat tgccattatc ggtggcggga tcattggtat gaccctagcc
     8101 agttacttag atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca
     8161 actaaagcta gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg
     8221 tatcgtctgg ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt
     8281 gatcaaacga tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct
     8341 gaattggctg ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa
     8401 tggctttcac ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg
     8461 cgtatcagtg gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt
     8521 ttagcacaaa aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat
     8581 tcttggctca tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct
     8641 ggcccggcac ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa
     8701 aaagggcagc tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct
     8761 atgcttgacg gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct
     8821 acccatgaaa acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg
     8881 actaagagta cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag
     8941 ttccgtgtag gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca
     9001 gatgatccaa gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct
     9061 tatatcggct atctattagc caacttctta aatactggtc attggcctac ggaagattat
     9121 caaaaggaca tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag
     9181 taaggatccc gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc
     9241 aatttctgaa tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct
     9301 atctgagctt caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag
     9361 gacgtatcct caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat
     9421 ggaccaaacg acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc
     9481 gtttcaggtt tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt
     9541 tgctgtttca aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg
     9601 aaaggattag caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt
     9661 tccgcgtctg atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca
     9721 ttgatcttga taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc
     9781 ttttcgctaa gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca
     9841 tttccgtttc gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg
     9901 cccacattct gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt
     9961 gttttatcaa agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg
    10021 aaagcatcgt catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc
    10081 gttgattttc cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta
    10141 gcatattgtt tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa
    10201 gcaagcgtca aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacagccgct
    10261 gtattatgga gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg
    10321 ggatcacaaa agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct
    10381 aagatcttcg aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat
    10441 ccttctggaa tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact
    10501 tttgtatggt aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg
    10561 tatttgcttt gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc
    10621 acaatgcttc catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca
    10681 gcatgtgaga ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa
    10741 gctgtcttcc tatcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc
    10801 gatcgaatac tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct
    10861 tttgtactca tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt
    10921 attcgagttt ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa
    10981 aaaaaccgag aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt
    11041 agtaccagcc gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg
    11101 ttacatattg ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg
    11161 aagcatctaa ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg
    11221 attctttttg tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg
    11281 gggcagcagg agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg
    11341 ctgtttcagt tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat
    11401 catttgaagt tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg
    11461 aatttttttc agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct
    11521 tagataatga atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc
    11581 ctacaaacat tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg
    11641 aattcatatt aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat
    11701 catttagaaa tatttcatag gtaaaagaaa c
//
LOCUS       DANKHL010000088        11677 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000088 DANKHL010000000
VERSION     DANKHL010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.091"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            4..1458
                     /locus_tag="PW101_001638"
     CDS             4..1458
                     /locus_tag="PW101_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDL2514038.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
     gene            1534..2694
                     /locus_tag="PW101_001639"
     CDS             1534..2694
                     /locus_tag="PW101_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDL2514039.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            2807..3814
                     /locus_tag="PW101_001640"
     CDS             2807..3814
                     /locus_tag="PW101_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2514040.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            3838..4863
                     /gene="iolG"
                     /locus_tag="PW101_001641"
     CDS             3838..4863
                     /gene="iolG"
                     /locus_tag="PW101_001641"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HDL2514041.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            4880..5770
                     /gene="iolE"
                     /locus_tag="PW101_001642"
     CDS             4880..5770
                     /gene="iolE"
                     /locus_tag="PW101_001642"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HDL2514042.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            5793..7388
                     /locus_tag="PW101_001643"
     CDS             5793..7388
                     /locus_tag="PW101_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDL2514043.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            7404..7718
                     /locus_tag="PW101_001644"
     CDS             7404..7718
                     /locus_tag="PW101_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL2514044.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            8179..8904
                     /locus_tag="PW101_001645"
     CDS             8179..8904
                     /locus_tag="PW101_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL2514045.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            9037..9879
                     /locus_tag="PW101_001646"
     CDS             9037..9879
                     /locus_tag="PW101_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL2514046.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            9879..10826
                     /locus_tag="PW101_001647"
     CDS             9879..10826
                     /locus_tag="PW101_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL2514047.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNI
                     EKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFS
                     KAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTN
                     IPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            10841..11071
                     /locus_tag="PW101_001648"
     CDS             10841..11071
                     /locus_tag="PW101_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HDL2514048.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            11406..>11677
                     /locus_tag="PW101_001649"
     CDS             11406..>11677
                     /locus_tag="PW101_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2514049.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFIN"
BASE COUNT         3715 a         1970 c         2609 g         3383 t
ORIGIN      
        1 taaatgacaa gatttttaag aaattatatt gatggtcgtt gggtcgctag tcgctcaaca
       61 gagtttgaag aagtaatgaa tccggctaca gaagaagcgc tggcgaatgt accgttgtct
      121 agcaaagcag agacagccga agcagttaaa gctgccgatg ctgcgtttca aacttggaaa
      181 aaagttcctg ttcctgttcg cggacgttat ctgttcaagc tgcatcagct tttaacagat
      241 cataaaagtg aattggcgcg tttgatcacg ctggaaaatg ggaaatcatt gtccgagtca
      301 ctgggagaag ttcaacgagg aatcgaaaat gtggagcacg cagcgggaat caccaattta
      361 atgatgggag attctttatc aacagcggcg actgatattg agatcacgaa ttatcgttac
      421 ccaatcggag taatcggagg gatcacaccg tttaattttc caatgatggt gcccttctgg
      481 atcttcccga tggctctggc ggcaggaaat acggtggtgc tgaagccttc agaaaaaaca
      541 ccattgttga tggagaaaat tgttgagctg gttgaaaaag caggctttcc tagcggtgtt
      601 tttaatgttg tctatggtgc ccacgatgtt gtcaacacat tgctgcaaga tccactggta
      661 aaaggcatca gctttgtagg atcaaaaaat gtcggtgaat acgtttaccg tgaaggaaca
      721 aaaaatctaa aacgggtaca agcgttgact ggagcgaaaa atcatgttgt tgtgctgaaa
      781 gatgccaata ttgatcgggc agtcaaggac cttattggcg gagcttttgg ttctgctggt
      841 gaacgctgca tggcagcctc cgtcattgtt ttggaagagg aaatcgcgga tcagtttgtt
      901 gaaaaattta aggctgcggc aaaagcgatc aagatcggca atggcttgga cgaaggggtc
      961 tttttaggac cagtgattcg aaaagatgca cagcagcgga cctttgacta tatccaaaaa
     1021 ggggtcgatg aaggagcgaa actggtacta gatggtcgcg aaaacattcc agaaaaaggc
     1081 tattttgttg gaccaacgat ttttgataat gtcacgatgg atatgaagat ctggcaggat
     1141 gagatttttg ccccggtcgc atcaatcgtg cgggtaaaag atttgatggc agccgttgag
     1201 ctgacgaatg aatctgaatt tgcgaatggt tcgtgtctgt ataccaacaa cgcatcagcg
     1261 attcgctact tcagagaaaa tgttgatgct gggatgctgg gggtcaatct tggggtgcct
     1321 gcaccgatgg cttggtttgc cttttcaggc tggaagcact ctttctttgg tgatttgcat
     1381 gccaatggca aagacagtgt tgagttttat acccatcgga aagttgttac agcacgttat
     1441 gcgcaaagtg agatttaaag atttgcttcg aagctctgtt aaagagcttc gaggattctg
     1501 ttataggcta taagccagag agaggttttt tctgtgaata ttcaactgaa aatgcctgcc
     1561 aatttaagaa ttggcggcgg aacaatccaa tttcttatgg aagaatcaga acaactaggc
     1621 aacaagccgt taatcattag tgatacggtt atggaacaga ccgggcatct tcaaaagatc
     1681 aaggagctgt tgggaaaaac agatgatcaa ttagcggagt atacagacat tcaagccgaa
     1741 ccgacgcaag aaaatgttta tgatgcgctg aagctttttc agaagcatca atgtgatcat
     1801 atcattgctt ttggtggcgg cagctgtctg gatacagcca aggtggtagc cgttttggct
     1861 gctaatcaat taagtctcga agcgattcat gcaagaaatt ttgatcagct gcaagcgggc
     1921 attccgctga ttgcagtgtc aacaactgct ggaactggtt cggaagtaac cgatgtggca
     1981 gttattacca atacaaaaac agcagtcaaa atgatgatga aggatctcgt tttcatgcca
     2041 cagatctcaa tcgttgactc tgattttacg aaaactgtac caccaaaagt aaccgcggca
     2101 acgggagtcg atgcgctatg tcacgctttg gaatcttatg tttcagtcaa agccaatgaa
     2161 gtaacgcaac tattttcact ggaagcgatg aagaaaattt tgaacacgct ttctcaggca
     2221 tatgaaaata gcgacgacac tgaagccaga gaacaaatga gcttagcttc aacggaggcg
     2281 gggattgcct tttcgaatgc gtcagtcaca ttgattcatg gaatgagtcg accggtgggg
     2341 gcactttttc acattcctca tgggatatca aatgcgatgt tattgccggt cttttttgaa
     2401 ttctcaaagg atgctattca gactgagtta gcgacgatcg ccagatttat ttatccggca
     2461 gccgaagatt tatcgctgga agaggctgcg gattatctga ttaaaaaaat ccaagagctc
     2521 tgtgagcagc tgtcgatccc aacgattcga gattatggta ttgatcagca agcgtttatg
     2581 gcagccttag acaaaatggc cgcggatgcg ttgaccagcg gaagtccgca aaataataaa
     2641 aaggtgccta gtcatagtca aattaaggaa ttatacgtac aagcttatgg gtagtaaaag
     2701 gcggagcaaa gagagggctc taccttttaa gaataaaagc tacacaggaa taattttgta
     2761 agcgcatact atgtgtgctt aaacaacaga acagggggat aagtaattga gagtaaaagt
     2821 aggcgttatc ggaacaggtg caatcggcat cgagcatatc gacaggatca atcagaaaac
     2881 acaaggggct tatgtgacag cagtctcaga tatcaatgta aaagctgcta aagaggttgc
     2941 caaacgtgtc gatgctaaat tctttgaaac cggagaagca ttgatcgctt ctgaagatgt
     3001 ggatgtcgtt gttgtgactt cttgggaccc gacccatgag cagtatgtgt tagaagcaat
     3061 caagcatggg aaatatgttt tctgcgaaaa gccattagcg atggatgcag caggctgccg
     3121 ccggatcgtt gatgcggaag ttgcttatgg gaagaaattg gttcaggttg gcttcatgcg
     3181 ccgctatgac cgcggctaca ttgaactgaa agaagcaatc gaatcgaagc aatacggaga
     3241 agcattaatg atgcattgtg cccatagaaa tcctagcgca gatgaaaact acacaacacc
     3301 gatggcgatc agcaacaccg cgattcatga gattgatgtg cttcgctggt tgctaaatga
     3361 agattatgaa tctgttcaaa tgatcttgcc gaaaaaaacc agcaactccc atgccgattt
     3421 gcatgatccg cagctgctga tctttcaaac aaaatccggt gtaacgattg atttggaaat
     3481 ctttgtgaat tgccgctttg gctacgatat taaatgtgat gtggttttcg aaacggcaga
     3541 agtaggaatg acggacccag cctatacacg aattaaatcg gaagaaaaaa attacacacc
     3601 tgtttcacca gattggcaga cccgcttttt ggatgcctat gattatgaat tcaagctgtg
     3661 gatcgattcg atcaaaaatg atgcggttgt cggtcctacc gcttgggatg ggtatgtagc
     3721 agcgatcacc atgatggctt gtcatgagtc tcgtgaaaca aaaacaaaag taatgattca
     3781 aatggatgaa cgaccagcac tatacaatga gtaattctca gaaaacggag gtatttcatg
     3841 aacaacatta gagtaggcat cgtcggttta ggaagattag gaaaagtaca tgcacaaaat
     3901 ttagcaacag gtgtcagttg ttgtgagctt acggctgcgt gcagtttagt tgaaacagaa
     3961 ttagcattcg cacagaagaa attaggcgtc aagcatactt tttccagcta tgaagaaatg
     4021 gtcaaaagtt ccgtgatcga tgcagtcttt attgtttcac catcaggctt tcactgtgac
     4081 caagtccgct tggcgatgga aaatggcaag catgtcttta ctgaaaaacc attaggcctt
     4141 gaagttgaag atattaaaaa gacccaaaaa gtcatcgaag agcatcctga acaggtcttc
     4201 atgttaggct tcatgcgccg ctatgatgag tcgtatcagt atgcgaagaa aatggtagaa
     4261 gatggcgaaa tcggcgaaat cacactgatt cgttgttatg ggatcgatcc gagcgaaggc
     4321 ttggaaagct ttgtcaaatt tgccatgaat aacgacagcg gcggcttgtt cttagatatg
     4381 gcgatccatg atattgattt ggttcgctgg tttaccggcc aagaagtcga tcaagtgtgg
     4441 gcgattggga aaaatgcggc gtatccagaa ttggatcagg ctggtgagct tgaaactgga
     4501 gccgcaatga tgagcctgag cgataaaaca atagctttac tagttgccgg cagaaacgca
     4561 gcccatggct atcatgtcga aacagagatc atgggaacca aaggaatgct gcgggtagcg
     4621 caagcgcctg agaaaaatct tgtgacgatc atgaacgatc aagggatcat ccgtccatgt
     4681 tcacaaaact tcccagaacg attcagagaa gccttcattc gtgaagccca agaatttgtc
     4741 tcatgtattc aagaaaaacg tcaaccagcg gttaatgcag aagatggatt gcaatcaact
     4801 aagatcgcgt tggcttgtca agaatcattc gatacgaaga gtttagtaca actaaaaaaa
     4861 taagcaggag tgtgagaata tgaccaaaca aattcaatta gccatcgcac caatcgcgtg
     4921 gaccaatgat gatatgcccg agcttggtag tgaaaatacc tttgaacaat gtatcagtga
     4981 aatggccctt gctgggttca ccggaacaga gattggcaac aaatatccaa aagatccaga
     5041 ggtgttggaa caatatttga atattcgcgg cttaaaggtt gccagtgcat ggttcagtgc
     5101 gtttctgaca actaaacctt ttgaagaaac tgccgaagcc ttcaaagcac atcgggattt
     5161 cttacatgcg atgggcgcta aagtcatcgt agtagctgag cagggtcata gtatccaagg
     5221 gattatgaca gcgccagtct ttgatgataa accagttttc actgatgaag agtggaaaaa
     5281 actggcggaa ggattagaaa aattagggga tttggcccac gaaaaggata tggagatcgt
     5341 ttaccatcat cacatgggta ccggcgttca aacgacggaa gaaatcgatc gtttaatgga
     5401 aatgactgat ccagacaaag ttaaattatt gttcgatacg ggacatttgg tcttttctgg
     5461 tgaaaatcca atcgcggttt acaaccgtca tcaagatcgt atcaaacaca tccatttcaa
     5521 agatattcgt caagaaacgg ctgaagaagt agaaaaggaa aaagacagct tcttaaacgg
     5581 cgtgaagaaa ggcgtcttca cggttcctgg tgatgggatg attgatttca ctccaatctg
     5641 gacagcaatc catgccagcg actatgaagg ctggatcgta gttgaagcag aacaggaccc
     5701 ggctaaagcc aatccatttg aatatgcagt caaagccaga agctacattc gagaggtcac
     5761 cggtctttaa tctattgaaa aggagctaga cgatgaatct tttttctata atcagtttcc
     5821 tagtgattgt catctgtgta tggttatttg cctattctag atccagaagt gtcgatactt
     5881 ctggatcaga agggttcttc atgggagggc ggagcttaac tgctttgcca atcgccggca
     5941 cgatcatcat gacgaacctg tccaccgaac aaatagtcgg acaaaatggt caaagttatc
     6001 atgcagggat ggaagtaatg gcctgggaag taactgctac gattgcgatc gtagctttgg
     6061 ctgtgatatt cttgccaaaa tattttaaat acggcatcaa tacaatctca gatttcatcg
     6121 agattcgtta tgatacggtt accaaacgaa ttatttcgat cttatttatt gtgacctata
     6181 tgacttcttt cttacccgtg gttctttatt ctggatcgct ggtgttcaat aagatcttcc
     6241 atattgatga gctgctgggc gtccagccga tcgtcgcgat cataattgta gcgatggcaa
     6301 ttggtattgt cggcatcctc tatctgctga tcggcggctt gtcgttaagt gccaatagtg
     6361 actccattta tggagtcggt ctgctggctt gtggtctgct ggtcccaatt ttaggcttaa
     6421 tgaagcttgg tgacggcagc tttgttggcg ggatcgaaca cgttgtggta aacacgccat
     6481 ggctgcttaa ctctgtggga tcgatcgact cggctgttgt tccatggccg acactattta
     6541 cagggatgct gtttaacaat ctgtatttct ggtgtaccaa tcaaatgatc gtccaaaaag
     6601 cattgtccgg caagaattta gctgaggcac aaaagggcgc tttcctagtc ggcgtcttca
     6661 aaatcttagg tgcgttattc cttgtctttc cagggatcgt cgctcgcaat atttttggcg
     6721 atagtttaat gtcaaatcca gataacgcgt atcctttttt agtcagtgaa gtattgccta
     6781 aaatgatctt tggtattttc gccgctgtta tctttggcgc gatcttatca tcatttgcgg
     6841 gagctttgaa cgcgacatcg accttgtttt cactggattt ttataaaccg cttatcaata
     6901 agaaagcaaa tgataagcaa atcgctcgtg ctggtaaaac agtaacggtc gttgtgggga
     6961 ttatctccat tatcgtagcg ccattcattt cttttgcacc ggctggattg tatcagttcg
     7021 ttcaagaatt caatggacta tacaacatgc cgctgctcgt gatcattctc tttgcctttt
     7081 attcaaaaaa agcaactgct tttggggcaa aaacgacgat tgccgtgcat atcgtcttgt
     7141 atgcactatc aaaaatgctt ttccaaaata tccatttcct ttatgttttg acggcattat
     7201 tcgttctgga cattatcatt ttgatggtgg tttccaaatt gaaacctgat ggagaatttg
     7261 tgttgaactc attcgataca aaggtcgata tcgagccttg gaaatacacc aaggtgactg
     7321 ctatagcatt ggtagtcatt gttattttaa cttatgtggc attttcacca attggctttg
     7381 caggctaaaa acaggagggt attatgcata aggatctatt gattatttgc gagaccggaa
     7441 tcagtgcgtc gctgctcgta tcaaaaatgc ttgaaacgat cagagccaaa aatctatcgt
     7501 atgagataga ttatgcaacg atccgaaacg ttgaagaaaa actgaattat aaagactatg
     7561 acgttttact tttaacgccg caggtcgcac gttctgagga aaaagtaaaa gaaatcatca
     7621 aaaaggaaaa atgtaccgct cagatcgttt atatcaaaca agaagatttt cgttatatga
     7681 atattgaaaa catcattgca agcatcgaag aagattaacg agaatctttg attattgagt
     7741 gtcgaggagg tggtatatga aaagatggca tgttcgcccc gatcacccca atatgaaggg
     7801 cggacttatt agaaataccc aatcttgtat ttattcaata gggtaagcgg acctcaatag
     7861 ccagctcatc cccaaaaagt cgaatggaga cacttaaatg agtgtaccta ttcgactttt
     7921 tgagactcta agtcaaatcg tgttgttctc attaaatcac tgaaaggaaa tgcttcggcg
     7981 tttccttttt tctttttgtt tttcttcgtg ttttgaaaat tacttactat ttactatacg
     8041 agtagtttca aataattttg acatcctaat cttcaacaac agaaaaaaat ctaaattata
     8101 aaatttaggg ttgactaaaa tataattttt gtataccata tgatcgtgat aatcattatc
     8161 atttagaagg agaaagttat gttagaagtg aaaaagctta atgtttgtta taacgatttt
     8221 ttagcattga acgagatttc tttaaaaata caagaaggtg ctttaacagg tattattggt
     8281 ccaaatggtg ctggaaaatc tacattgctt aaagcaatgt taaatattat ccctcatcag
     8341 ggagaggtct ccattaataa tgaagatatc aataaaaaac tggtgaagat tgcatatgta
     8401 gagcaaaaag ctgatattga ttttactttt ccaataaaag taaaagaatg tgtatctatg
     8461 ggaacatatg ccggaatgaa agtttttcaa agaattaaaa atgcagaatg gcaaagagtt
     8521 tcaaaggctt tggaaaaggt cgatatggga aaatattcta atcatcaaat aggagaatta
     8581 tctggaggac aatttcaacg tgtactttta gcaagatgtc ttgctcaaaa tgcggatttc
     8641 atatttttag atgaaccatt tgtagggata gacttagtta gtgaacgaat aattatggat
     8701 acgttaaaag aattgaaaca tcagggaaaa acagtgctaa ttgtgcatca tgatttaagc
     8761 aaggtaaaga agtattttga taatattatt cttttgaatc gaaatctaat tgctcacgga
     8821 agtgtagaat ctgtatttaa tgaagagaat ttaaaaaaag cctatggaga tactatattt
     8881 attggagagg ggggaacggc ttagcctctc aatccaataa atatattatc tcaaaagaaa
     8941 tgagttaata ccaaattaaa ataacgatgg ttcatagggt tatccaatca aaaccggaag
     9001 gttaaaaccc taactctaat atacaagggg aataatatgc ttcaaaattt tattaatggt
     9061 ctgtatgact ttcacttttt acagaatgct ctaattacat ctgtagttat agggattgtt
     9121 tctggaacag taggttgttt tattatttta agaggaatgt ctcttatggg tgatgctatt
     9181 tctcatgctg ttttgccagg agtagcaatt tcttatattt taggtattaa tttttttatt
     9241 ggtgcgattg tatttggtct attagcttct atcattatta cttttataaa aagcaatagt
     9301 gtaataaaag gagatacagc aattggtatt actttcagtt cttttttagc attaggagtt
     9361 atccttatag gagttgcaaa tagctcaacg gatttatttc atatcttatt tggaaatatt
     9421 ttagctgtac aagatattga taagtggatt acaattggcg tttctgtttt agtgttggtt
     9481 acagttgttc tattttttaa agaattatta atcacttctt ttgatccatt aatggcaaaa
     9541 gcaatgggga tgaatgtcag tttttatcat tatctattaa tgattctatt aactttagta
     9601 gcagtaacag ctatgcaaag tgtgggaact atattaattg ttgcaatgtt aattacacca
     9661 gctgctacag catatttata tgcaaacagt ttaaaaacta tgatatttat ttcggcgaca
     9721 gtcggagcgt gctcatcatt gctaggtttg ttcattggtt atagttttaa catagcagca
     9781 ggatcaagta ttgttttaac agcggcattc ttatttgtaa ttagttttct aatctcacca
     9841 aaacaaaaat ttttaagaaa gaaggagaga aagttatgat gagaaaatgg aaagcagtac
     9901 tgggaagtct ggggattttg attgctcttt ttatattcgg ggcatgttca acaaatagta
     9961 aagacaaaga tacagtggct tcgaacgaaa aattaaaggt agtagttact aattcgattt
    10021 tagcagatat tactgaaaat atagcaaaag ataaaattga tttacacagt atcgtaccta
    10081 ttgggaaaga tccccacgaa tatgaacctt tgcctgaaga tgttcaaaaa acttcaaaag
    10141 cagatttgat tttttataac ggtgttaact tggaaactgg aggaaatgct tggtttacaa
    10201 aattagtaaa aaatgcgaac aaagaggaaa acaaagacta ttttgcagca agtgatggca
    10261 tagatgttat ttacttagag ggtcagagtg agaaagggaa ggaagatccc catgcttggt
    10321 taaatttaga aaacggtatt atttacgcta aaaatattga aaaacagtta gcggaaaaag
    10381 atcctgataa taaaaaattc tataaagaaa atctagataa gtatattgaa aaattggatt
    10441 ctctagacaa agaagctaaa tctaaatttg cttcaattcc gaatgataaa aaaatgattg
    10501 ttacaagtga aggatgcttt aaatatttct cgaaagcgta taatgtgcct tctgcttaca
    10561 tttgggaaat caacactgaa gaagaaggaa caccagatca aataaaacac ttagttgaaa
    10621 aattacgcac aacaaaagtt ccctccttat tcgtagaaag cagtgtggac gatagaccaa
    10681 tgaaaacagt atcaaaagat accaatattc ctatctattc aacgattttt actgattcaa
    10741 ttgcagaaaa aggacaagat ggtgatagtt actatgcgat gatgaaatgg aacctggata
    10801 aaattgctga aggcctttcg aaataatttg gagggtaggt atggtacaaa ttaaaacaac
    10861 tactatacta atttatttac tagcaattca ggaattatca aaaagaaaaa agaaattaaa
    10921 aaataatgat ttagcaaaag ttctaagtgt tagtccagca tctgtaagtg agatgctgac
    10981 taaacttaga tatgaggact atttagatat aggatttcaa ttaactgata aagggaaaaa
    11041 atttattgac gattacaaaa aaaggtttta agtaaaaggc tttattttta gggaggataa
    11101 gaaaatggct attggagtga ttatgatagg acttatccta atagctggat ataatttttt
    11161 taaatctttc tcggattatt ttagaaaagg tgaatgattt agatgagtct attaattctt
    11221 gtaggattgc tttttgtatc agttattttg ctctttctca ttagtttagc tctaaggaat
    11281 gagttcagta atagacgtta acgattgaaa cttcaagtcc tgtgtaagat aatatagtta
    11341 attcatctta ttaggaggaa gtacccatag aaaccagaga ttatattaca actcaccaaa
    11401 aattaataga aagtggacta agccattttg aaaaagtagg atttcagcaa actaagataa
    11461 ggcaactgtg cttgaccgca ggcgtaacta cgggtgcttt ttataaacat tttacttcta
    11521 aggaagcgtt atttgaagaa attattcagc cgcatattaa tgcgcttatt aatatgtata
    11581 atgaagagtt taccaaatac ctcttaaact tagaaaatat agatatagag aaaagttgga
    11641 gagagaactt caatgaatta gatccattta ttaatta
//
LOCUS       DANKHL010000089        11640 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000089 DANKHL010000000
VERSION     DANKHL010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.092"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            132..3659
                     /locus_tag="PW101_001650"
     CDS             132..3659
                     /locus_tag="PW101_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDL2514050.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3656..7378
                     /gene="addA"
                     /locus_tag="PW101_001651"
     CDS             3656..7378
                     /gene="addA"
                     /locus_tag="PW101_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDL2514051.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7447..7788
                     /locus_tag="PW101_001652"
     CDS             7447..7788
                     /locus_tag="PW101_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2514052.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8097..9140
                     /gene="pheS"
                     /locus_tag="PW101_001653"
     CDS             8097..9140
                     /gene="pheS"
                     /locus_tag="PW101_001653"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDL2514053.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9145..11565
                     /locus_tag="PW101_001654"
     CDS             9145..11565
                     /locus_tag="PW101_001654"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDL2514054.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
BASE COUNT         4017 a         1988 c         2503 g         3132 t
ORIGIN      
        1 ttttgcgtat acttaaaaaa gtagaaatat cagaacctta aaaggatata atttgaagaa
       61 tatagaacaa atgttcagta ttttttgata taataaagga gcaccgtatc atgtgttgaa
      121 ggaggaaaag catgtcttta caatttattc ttggaaacgg gaaaaaagat caccgtcaag
      181 tcttgatcga cgaagcatcc gcttggctcg accaggatgc aagtaaccaa gtatttttcc
      241 ttgttcccaa ctacagcaaa ttcgaacagg aacaagaaat tttagcggag atgcgaagaa
      301 gaagcggaaa aaaaactttc agcacaacaa acgttcaagt atttagtttt tatcgtctgg
      361 cttggtattt gctgcaacag acaactcttc ttacaggaaa tgaattatca gaatcaggat
      421 ctgcaatgat cttgcgacag attttagaaa aaaatgagga agaattaaca atctttcgcg
      481 gagaaatcaa taaaagaggg tttattcgtc aattacaaga actctacagt gaattgcgta
      541 caggaaatat tcaaccagaa gacctgatgc ttttatttgg tgattcacct aatgcagaag
      601 accagcaact taaaatgaaa gacttgaagc tgattttttc agcttatgac agcgaattga
      661 ctcaacgtgc actaacaaat gaagatgcac tatctatact agcaaattat atgcagacac
      721 aagatttcaa caacgtcaaa tttattgtca gtggtttttc tcgaatcaat ggtcaagagt
      781 atcaattact gcaggcaatg atggaaaagg ggcaattggt tgtcgattta ttgctggatc
      841 gtccttatac agcggatctt ccagaagtac ttacgctctt tcatgagact ggaaagcttt
      901 atttccgtct ttttcaaagt gcaagagaac ggaaagtccc tatattagtg gaccgatttg
      961 caccagagca gactcgtccg acagagcttc aactgctgca aacaatgtgg gaagagacat
     1021 ctagccaaaa gaaagcggca gtacacaaaa tagggggaca agagcatctc catttgtggg
     1081 aagctgaaaa tccaactgaa gaagtccgac aattagctgt tgaaatcaga cgactagtca
     1141 gtgaagaaaa ctaccgttat cgtgatatcc aagtgctgac aaaaaatatg tctttatatg
     1201 gaaatatcct tcggccgatc tttcaagaaa tggcaattcc tttttatatc gatgaagaac
     1261 aaatgatgga aaatcatccg ctgatcgaat ggatcaacag tctgttcgct cttgaccgct
     1321 attcttatcg gctaagtgat attatgcgtt ttttcaaaac tgaattattt ctgtcgacgg
     1381 aagcagaaac cgaacttgat gaatggaaac aagagcgaga tgaattcagg aaaaaactgg
     1441 acatcactga aaatgtagca cttgcttaca attatcaagg aacttattgg actcgtgaaa
     1501 aagactggca gtttgtggcg tatgattttg aagcagaaca gattgaagac gtacagtatt
     1561 tggaagaaca gtcaaatacg attagacgat tatttcagaa agctgtacca gtatttttga
     1621 aaaaaataaa gaaaagcaaa acaggacaag aggccgcaaa tgttttttat caatttttga
     1681 tccaaagtgg cgtacagcaa caattattgt tttggcgtaa tcaggaagta gacagaggaa
     1741 acttagaagc ggcaagaaac catgagcaga catggggagc gctgattgat ctgttggatg
     1801 aatatgtagt aatttatggt gaatcggctt ttgactggtc actgttccaa gaaatcatta
     1861 caagtggtct agaaaatcta agttatggta aaatccctac cgcaatcgac caagtgcgaa
     1921 tcaatcgttt ggaattagtt cgaccaaatc aagctgccgt cacttttgca ttagggttga
     1981 atgatcaagt gtttccagac cgaaaagaga caaaaggatt gttgtccagt gaagaacgag
     2041 aagtattaaa tcaggccttg ccagatggac agtttttatt cgacccatca aaggaaagta
     2101 tctcttttga gcctttccaa gcctatcttg tttttctttc cggaacagat cgattatatc
     2161 ttagttatgc acaaagctat gacacagatg gttcgctgaa gatgtctcct tatttgcgta
     2221 gaatcacaga atatttatct gttcccgtcg agagaaaaga gcatttgacg atcgaaagcg
     2281 accctaattg ttatgtaggg acttacagaa gcggaatcaa tacagtcaat aggatttatc
     2341 gattagcttt agatgaaaaa aaacctgtac caaagcactg gcaaaagctc aaagaattag
     2401 tactgaattc gtcatggcga aattttgctt tacagatttt tgaaagtcag gaacatttaa
     2461 atgttcctgt ttcgatgcca gaaaaaatgg cggaagagct ttatggaaaa gagatttata
     2521 cttcaatttc acgtatggag aatttttata attgtgaata caaatacttc atgcaatttg
     2581 gtttgaaatt gaaagaacga actgtttatg gtctaactcc agcagcaact ggtgattttt
     2641 atcatgaatc attggatcgt ttcttcaagc tactgttttc aaaccaatta tccttagtga
     2701 atatgtccga tgcgcagcgt agagacttta ctgaaaaagt gctgcaggaa gtattcggag
     2761 aacttcggtt tgagatattg gatagttctg caagaatgaa ctatatccgc taccagttag
     2821 gacagacgat tcaaaaagtg acatgggctt tgcaaaaaca gagcaaaaat acaggcatgc
     2881 atcctcttca gacagagatt ctatttggtc agattgctgg agcaaaaggt atcacaggtc
     2941 tggaattacc gttgagtaat ggtggaaaac tccatgttcg agggaaaatc gatcgaatcg
     3001 atgtagcttc tgaacaagaa gatacttggc taagcgtcgt tgattacaaa tctagcggac
     3061 gttcattcga tgtcacagaa gcttattatg gcatggctat gcaattattg acgtatctgg
     3121 acgtagcatt gatggatgcg gtacgtttga ctggaaaggc ggctgttaaa ccagcaggat
     3181 catattattt acatgttcat aatcctgtat tgacggagac agatgatgta gagaagaata
     3241 cattgaaaaa atttagttat gatggcgttt ttgtcgatga tcccgctctt ttagaagtac
     3301 tagatcattc actggaagca aaagaacaat cactgatcta tccagtgaaa aaaaatgcga
     3361 aagatgaata ccaaaaaggt agtttcagta aagacaaatt tttcacggaa gaagaactgc
     3421 aactattcat ggcgcataat cgggaaaata tgcggttagc tggagatgcg attacttccg
     3481 gagaaatcaa actaaatcca gcttataaag ataaacagcg gattgcctgc gaattttgtc
     3541 cattcagaag catctgtacg tttgatgtga tgctgaaaga aaataattac caccgtctgg
     3601 agaaaataga taaggaagaa gcactgcgta gaatattgaa aaggagtgaa gaagagtgaa
     3661 tcaaattcct ttaaaaccgc aaaatgaacg attcacagat gatcagtggc aagcgatttt
     3721 tgatcaagga gataatctat tagtctctgc gtctgccgga tcaggtaaaa ctacagtgct
     3781 tgttcgaaga gtgattgaaa aactaaaaat gggttttgat atcgatgaat tattgattgt
     3841 gacatttact gaagctgccg caagagaaat gaaagaaaga attcaagagg cattacaaga
     3901 atccgtcaat agcgaaagtg accctgtacg tagacagcat tttactaagc agttagtttt
     3961 attgcccaca gcgaatatct caaccttaca tgctttttgc ctgacagtta ttcgacgtta
     4021 ttattactta attgatattg atccagtctt tcgcatgtta acagatgaaa cagaaacgat
     4081 cttgatgaag gaggatgtgt gggacgagct gagagaagca ctttatgcag aaaatgatga
     4141 acgatttttc caattaacaa tgaatttttc taatgatcgt tcagatgacg gcttgacgaa
     4201 tctggtgttt tctctttatg agtttgcccg tgccaaccct gacccccaaa aatggctaga
     4261 acagttaagc gataattatc gtttgcctga ggggctagcg aaatctcgat tatatcaaaa
     4321 acaaatccgt cctttagttt tggcagatat ataccaatgt gtacagttat atgaacagat
     4381 gactcagtta gcacaaggtg aaggattaga aaaaatgcat gagcaagtag ctggagaaca
     4441 acagcagatc aagaatatct atgaagcgtt ctcgcaagat cggttagaag aagcttatgc
     4501 tggtttggaa caactaactt tttcaacttt taagagcagt cggaaagcag aattgaaaga
     4561 aatttccaat gaagtcaagg gcatgaggga taaagcaaaa aaactgattc agcagataag
     4621 taaaagttat tttcctgttt ccccaagcca aatggaagaa ttaacggaca aagcactacc
     4681 gttagtcgaa gaaatgacaa aagtcacgca aagcttcatg gacggtttca gtatgcgtaa
     4741 acgggaaaaa ggtgtgctgg atttcaatga tctggaacat ttagcgttgc agattctcac
     4801 agaaaaaacg aaagatgtat ggctgccaag tgaagcttca aaacattatc gaaaaaaatt
     4861 caaagaagta atggtagatg aatatcaaga cgtgaaccaa ttacaagaag ccattttata
     4921 ctggctaaga gaacccgatg atacaaaagg aaatatgttc atggttgggg atgtcaaaca
     4981 atccatctat tctttccgtt tagcagatcc tagtttattt atcgggaaat acgagaattt
     5041 ctcaaagaaa gaaggcggac gtcgaatcgt tttggctgag aacttccgct ctagaaaaga
     5101 agtcctttct tttacgaatc ttatttttga acaactaatg gatccagcag tgggtcaaat
     5161 caattatgac gaagcagcca aactgatcca aggattttca gactttccag agaatgaaca
     5221 gtttgagccg gaaatcatga tctatgaaaa agaacaggaa gaatcagaga ttgagattcc
     5281 gacggatgat atactggaag acaaaacaga aggcgaactt tttatgacag gactaaaaat
     5341 tcgtcaatta attgattcct cctttatgat ttatgataaa aaatcaaaaa aaagtcgtcc
     5401 gatagagtac aaagacattg ttctcttgac acctacaaag aagaacaact taacgatttt
     5461 agagattttc aaaacattgg atattccatt agagatgaat gatgctcaaa actattttca
     5521 agcgacagaa attcgcacga tgatctcgct gctacaatta atcgataatc cctatcaaga
     5581 tattccgctt gcggctgtat tgcgctcacc gatcgtgggt ctgattgaac cagaattagc
     5641 cagcattcga ttagctaacc gtgcacatac ttactatgat gctgtgcttg cttatcaagc
     5701 atcaaacgaa gatgaattag cagctaagct ggaacatttt ggcaagcaac tagagcattg
     5761 gcgagaactt gctcgccgtt cttcgatcac tgacttgtta tgggatatct attatgagac
     5821 gggatatcta gagtatgtgg tcggtctgcc agcaggggca cagagacaag ccaatcttta
     5881 tgcattggtt gatcgtgcca aagcttacga gcaaagttct ttcagaggat tataccagtt
     5941 cgtgcgtttc attgaaaaaa tgcaagaaaa agataaagac ttagcagaac cagtcatttc
     6001 aatagaagat aatgctgttc gcgttatgac gatccatgca agtaaaggct tggaattccc
     6061 agttgtattt ttgttagata tgacaaagga gttcaactta caagacttgc gcaatcggta
     6121 tgcttttgaa gaaaaacttg gagcaggaat tcgctatatg gatcctgaaa cacgagtgtt
     6181 atatgatacg ttaccgtttc aagccatcaa attggcaaag caaaacaaat tgttgtcgga
     6241 agaaatgcgt aaattgtatg taggattgac tcgggcagaa caaaagcttt tcattgtcgg
     6301 ttcctataaa aataaagagc aaatgatcca aacgtggtct gaagcagctg atcatgagga
     6361 gttagttttt gatcctgcgc tacgtttgaa gggcagaagc agcttgatga attggattgg
     6421 ttacggattg atccgtcatc ctgaaatgca gaaatactta gaggaagaaa tttcgacttc
     6481 tcttcttcaa cattcaaatg cacagttttc tatttcatgg atgaatcagc aatcgatcat
     6541 tgaacaaaga cagttgcttg ctgaaaaaga actggttaac ttagatcagc agatgaaaga
     6601 agatgaaact ttattagcag attcgttaca aaaacgtctg gcatatgaat atccttatca
     6661 agcttcttca caaaccacaa gttatcaatc tgtatctgaa atcaaacgat tattcgaaga
     6721 tccagatgat acacaagaat cacgtttgac tctggaaagc agtcagaaca aagcagcatc
     6781 ccgtcaattt cgttatacgc aagaacaact agcagaacca aaatttttgc aaaaagatcg
     6841 gcaagtgtca gcagctgctg ttggaactgc tacgcatgct ttgttacaat tactgccatt
     6901 agagatgcca actacagaga gtatccatca aaaattacag gaattggtaa ggaaacggtt
     6961 agtagacgaa aaagtcgcga aaaaagtaga tgtttcttct attatatggt tctttcaaac
     7021 agaacttggt caacagctga ttgccaacaa agaaaacgta aaacgagaac aacctttttc
     7081 catgctgctt cctgctgacg aagtatttca agactatcca aatcaagaag atgaactatt
     7141 aatccacgga atcgtcgatg gatacctaga agaaaaagat catctcaata tctatgattt
     7201 taaaacagat tttattttac atccggatga tccagcagaa atagatgcaa tcgtccaaaa
     7261 atatcaagga cagctaagac tttaccaaca agcaatgtca gaagctttga ataagccagt
     7321 agaaaatgtt ttcctaattt tgcttcgagt gaaacaaatt ataaacatta acaaataatt
     7381 ataattgagc aaacttgact tatgttttgt aaggattatg ctatactctg ggaagaggtg
     7441 agtatgatgg agctaacaca gacagaattc gttttatgcc ctaaatttga aaaaacattc
     7501 tctattttag gaaaaaaatg gaacggattg attatcgatg tactactcga aaacggtccg
     7561 caacgctttg ttgagcttgc ggcaaaaatc ccgatggtca gtgatcgagt attagtcgaa
     7621 cgcttgaagg aattagaaaa agaaggaata gtcactcgaa actttgcctg tagcgaaaag
     7681 agccgttcag aatatctctt gactgaaaaa ggagcagact tgcaaaaagc gatgcgtcaa
     7741 atacaagttt ggagcgaaaa atggatcaca gaggaagaat gcggttgatt ttgtaaaaaa
     7801 aatcttgtaa actatagtcc atacgaataa gtaaagaacg atgacgaaaa agagtagttt
     7861 ggctaaacca gttcagggag atctgtcgca gactgaaagc agatcctgtg tttgaaaaat
     7921 gaagttcact ttccgagttc gtctgataaa atcagaccgg caattgtcgt taacgaagta
     7981 agagtgcggt gtaagtcatt tatccgcaaa ttaggatggt aacgcgaaga cctcgttcct
     8041 taacaggaat gaggtctttt tgttttgtat ttggaattta aaggaggaac aagcacatgt
     8101 ctctacaagc acaattagag agcttgaaag aagcaacgct cgtcaaaatc acagcggcac
     8161 aagatttaaa tgaattaaat caaatacgtg ttgaaacgtt aggaaaaaaa ggaccgatca
     8221 cagaagtatt acgtgggatg aaagatctaa gtccagaaga gcgaccaaaa gtcggaagtt
     8281 tcgcaaatga gatccgagat cttttgacag aagagatcga gcgaaaaaaa caagtattag
     8341 aagaagaagc gttaaatgca gctttggcaa aagaaacaat agacgtgaca ctacctggta
     8401 gaaaagtaaa acatggtaca cgtcatatcg tgacacaaat catggaagaa attgaagata
     8461 tttttgtggg tatgggctat cagatcgtag aaggatatga agtagaatct gatcattaca
     8521 atttcgaacg gatgaatctg ccaaaagatc atccagcaag ggacatgcaa gatactttct
     8581 atatttcaga cgagatattg attcggacac atacttcacc agtccaagca cggacaatgg
     8641 aaaaacatga tttctctaag ggtgctctac gaatgatctc gccagggaaa gtcttccgca
     8701 gagatacaga tgatgcgacc cacagtcatc agttccatca aattgaaggg ctagttgttg
     8761 ataaaaacat cacaatgggc gaccttaaag gaacattaga agtcgtaatg aaaaaaatgt
     8821 ttggggaaga tcgtgaaatt cgtttgcgtc caagttattt cccatttacg gaaccatcag
     8881 tagaagtaga tgtcagctgt ttcaaatgtg gtggtgccgg ttgtaacgta tgtaaataca
     8941 ctggatggat cgaaatttta ggagctggca tggtacaccc gaatgtgttg aagatgtcag
     9001 gaatcgatcc agaagaatat tcaggctttg ctttcggcct tggaccagac cgcgtagcta
     9061 tgttgcggta tggagtgaat gatatccgta atttctatca aaatgactta cgtttcttaa
     9121 gtcagttcaa gggggagtaa gaaaatgtta gtttcatata aatggctcaa tcgttatgta
     9181 gatttatcga atgtaacacc aaaagaatta gcggataaga tgtctgtgac aggaatcgaa
     9241 gtagagggag taacggttcc agaagaaggc ttgaaaaaaa tcgttgtcgg tgaagtcaag
     9301 gaatgcgtac cacatccaga ttctgatcac ctttctattt gtcaagtcga tattggcgaa
     9361 gacgaactgt ctcaaatcgt ttgtggagcg cctaatgtga aggttggaat caaagtgatt
     9421 gttgcacttc ctaactcacg tatcgctggt aatgtaaaaa tcaaaaaagg aaaaatgcgt
     9481 ggccaagtat caaatggcat gatttgctca ttacaagaaa ttggctattc agatagtgtt
     9541 ataccgaaag aatacgctga aggtatctat tatatgccac aagaagctgt aaatggcgat
     9601 ccagtttttc cttatttgga tatggatgat tcgattattg aattatctat cacaccgaat
     9661 cgtgcggatg cattgtcaat gcgtggcgta gcttatgaag ttggggcaat ttatcgtcag
     9721 actcctacat tcgatgacga aaagctggta gaatcctcac gtcctgctag cgataaaatt
     9781 caagtggaag ttgaagataa agaagcagtt cctgcttatc aaatcagaat cattgaagga
     9841 gtaagaatcc agccaagtcc tcaatggctg caaaatcttt tgatgaacga aggcattcgt
     9901 ccaatcaaca atgtagtaga tgtgacgaac tatattttgc ttttattcgg acaaccacta
     9961 catgcatttg actacgacaa attgaaatca aaagagatct ttgtccgccg tgcaaaagaa
    10021 aatgaaacat tagtaacact agatggagaa tctcgggaat tgacaactga caatctcgtc
    10081 attactaacg gcaaagaacc agttgcttta gctggtgtca tgggagggtt agactcagag
    10141 atcacgaatg aaacaacaac agttgcacta gaatctgctt tatttgatcc aatttcgatt
    10201 cgtcgtacag caaaacaatt taatttgcgt agtgaatcct ctgcacgttt tgaaaaagga
    10261 atcaaccatg cgactgttgg agaagccagt gaggttgctg cagcaatgat cgtttctctt
    10321 gcgggtgggg aagttctaca aggcgcggtc aaaggctcag agatcaaagc agaagacgta
    10381 tcagtgaaga tttcacttga acgcatcaat caatatttag gaacatctct tgtcgttaaa
    10441 gaagtaaatg agatttttga agcacttgga tttagctatg aagaaaagga gtatgtttat
    10501 tccgtagtag taccaccaag acgctgggat atccaaatcg aagcagatat tatcgaagaa
    10561 gttgctcgta tttatggata cgaccagttg ccatctacac ttccaagtgg agaaacggtt
    10621 gctggaagct tgtcaaaaga acaagaaact actcgtaaaa ttcgtacaat ctttgaagga
    10681 agcggattga gcgaagcaat cagttatgca ttgacaacag aagaaaaatc aagacagttt
    10741 gttataaatg aaagtaatat tacacgtttg gactggccaa tgagcgaaga acgttctgta
    10801 ttacgaatga atttgatcag cggactatta gatgatatca gttataacac tgcccgaaaa
    10861 aatacagaga ttgctttata tgaaattggt cgggtgttct tccaagaaaa tgatccacgt
    10921 acacatctgc cgcaagaagt aaaacatgca gcaatcgcta tttctggtat ttgggaagaa
    10981 aaagattggc agacgaaagc tcaaaatgta gactttttca ccatcaaggg tcttttagaa
    11041 aacttatttg aacaactagg catcactgaa gatgtccatt atcaaacagt gacagaaatg
    11101 aaggaaatgc atcctggaag aactgctgcg atttaccttg gcgaaaaatt tattggtttt
    11161 gtcggtcaag tgcatccaac gattacaaaa agttatgata ttccagaaac atatgttgct
    11221 gagtttgatt tatctgctat agtggaagct gctcaaaaag ggatcgtttt cgaagcagtg
    11281 acaaaattcc cagcagtcag ccgggacatc gctttgcttg taaaagaaac tgtgacaaat
    11341 caggaattaa cagaagttat ccgttctgct gccggaagat ttttaacaga tatccaatta
    11401 tttgatgtct atcaaggaga aaacattgaa aaaggacata aatccatggc gtatagcctg
    11461 acattcgtca acccagaagc aacattgaca gatgaagaaa tcaataaagc aatggaaaaa
    11521 gtcgaaaaag cgttggtaga aaacctagag gcaagtattc gctaagaagg aaaatatagt
    11581 aagtaacgat caagtagctt ttgaagacca tacatttgaa aaaaagatgt gacacaactt
//
LOCUS       DANKHL010000090        11435 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000090 DANKHL010000000
VERSION     DANKHL010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.093"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            818..2428
                     /locus_tag="PW101_001655"
     CDS             818..2428
                     /locus_tag="PW101_001655"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDL2514055.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            2720..3589
                     /locus_tag="PW101_001656"
     CDS             2720..3589
                     /locus_tag="PW101_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDL2514056.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            3754..5025
                     /locus_tag="PW101_001657"
     CDS             3754..5025
                     /locus_tag="PW101_001657"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL2514057.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            5022..6317
                     /gene="rho"
                     /locus_tag="PW101_001658"
     CDS             5022..6317
                     /gene="rho"
                     /locus_tag="PW101_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDL2514058.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            6416..6679
                     /locus_tag="PW101_001659"
     CDS             6416..6679
                     /locus_tag="PW101_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDL2514059.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            7124..7573
                     /locus_tag="PW101_001660"
     CDS             7124..7573
                     /locus_tag="PW101_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2514060.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            7630..9048
                     /locus_tag="PW101_001661"
     CDS             7630..9048
                     /locus_tag="PW101_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2514061.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            9048..9908
                     /locus_tag="PW101_001662"
     CDS             9048..9908
                     /locus_tag="PW101_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDL2514062.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(9985..11286)
                     /locus_tag="PW101_001663"
     CDS             complement(9985..11286)
                     /locus_tag="PW101_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2514063.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         3732 a         1975 c         2472 g         3256 t
ORIGIN      
        1 accgtttatc cagaggttgt gagcctacca tttaactccg aatcacaaga aataatttca
       61 aaaacagctt ctcatgtttg atggatattg tgacttgtga ccgaggagtt ggcaggagaa
      121 caccgtttat ttagaaataa ggaggcacga cttttgtcgc atctccttat tttttactta
      181 tgtgattcgt taccttcaat gaatccctac aaggcacttt tgttgaaaaa aaggtcatct
      241 ttgaaaaaat tagtggaatt gttttgtttt tgatattatt taatagaaaa gatatgccaa
      301 aaatccgaga actgtggact acgtttttta cgtaaaggag gtaaataagg gtgaaagaaa
      361 aagattgttt ctacgataag ctatataaac tgttgctgca agtaggaaaa agtagttctt
      421 tgttaggtgg gatattatct atcataatgt ttgttttatt tttttatcta ttatttcact
      481 taaaattttt cagtaacgat tttctgattt gcttaggttt aacgttagct atgtttatat
      541 caagcttatt atgtgggtta aatttttttt gcactcataa aggacttaag aaataatttc
      601 tcaaaggata tcgaaaaaga aaaaattgta agcggatagt attcatttga gtacttgact
      661 ttcccttagt cagcttgtat tatacacctt gggcaccctt ttagagggtc agaagtttac
      721 agaaatttcg gctccctatt cttattgaag aatagggagc tttgtttatt gtctgagact
      781 tttcctgcac ataaattaaa aaggagtttt tgaacttatg acaaaatata tttttgtgac
      841 cggcggtgtc gtttcatcaa tcggaaaagg gattgttgca gcttctttag gacgcttatt
      901 gaaaaaccgc ggcttaaaag taactatcca aaaatttgat ccatacatca acgtggatcc
      961 ggggacgatg agcccatatc agcacggaga agtattcgta acagatgatg gtgctgaaac
     1021 agatttggat ctgggccact acgaacgttt tattgacatc aatttgaaca aatactccaa
     1081 cgtgacgaca ggaaaaatct attctgaagt tttgcgtaaa gaacgtaagg gcgaatactt
     1141 gggagcaact gttcaagtta ttcctcatat tacaaatgag atcaaagaaa aaatcatgcg
     1201 agcagctaaa atgactgatt cagatgtgat cattacagaa gtagggggaa cagtagggga
     1261 tatagaatct ttgccattcc ttgaagcact tcgtcaaatg aaagcagatg tcggtacaga
     1321 taatgttatg tatatccata caacattgat tccttacttg aaagctgctg gagaaatgaa
     1381 aactaaacca acgcagcaca gtgtaaaaga gctacgtggt ttaggtatcc aaccgaatat
     1441 tttggtggta cgtacagagc aacctgtttc tcaaagcgtg aaaaacaaat tggcacaatt
     1501 ttgtgatgtc gaaccagaag cagttatcga gtcgccagat gtagatacac tttactccat
     1561 cccattagtt ttacaagcac aaggaatgga tcaaattgtt tgcgaccatt taaaactaga
     1621 tacaccagaa gcagacatga cagaatggaa agaattagaa gaacgtgtct tgaacttgaa
     1681 gaaaaaagta cggattgcgc ttgtcggtaa atatgtcgag ttaccagatg catatatctc
     1741 agtagttgaa gcattgaaac attctggttt cgcctataat tcagacatcg agatcgattg
     1801 gatcaaagca caagaattaa cgagagaaaa tgtagaagaa cgtttgaaag acgcagatgg
     1861 aatcttggtt cctggtggat ttggtgatcg cggagtagaa gggaaaatcg aagcgattcg
     1921 ttatgctcgc gaaaatgacg ttccgttttt aggtatttgt cttggtatgc agatggcttg
     1981 tgtcgaattt gcacgtaacg tcgttggatt agaagatgcg tcatcagctg aaacgaatcc
     2041 agatacagca aacaatatta ttgatttaat ggctgatcag gaaaacatcg agaatttggg
     2101 tggaacttta cgccttggat tatacccatg taaaatcaaa aaaggaacga agacggctga
     2161 agcatatgat ggagccgatg ttgtgcaaga acgtcaccgt caccgctatg agttcaataa
     2221 caaatatcgt caattgtttg aagaacaagg gctagtcttt tcaggtgtct caccagataa
     2281 ccgtttagta gaaatcgtag agctgccaga gaaaaaattc tttgtgggat gccaattcca
     2341 cccagaattg atttctcgtc caaatcgccc gcaaaaatta atcaaagcat ttgtcggagc
     2401 atcattagca gatcacgaaa caaaataatt tttgaacttg atagaaagag aaaagtagca
     2461 agagaagggg ataagcaatt ttcttgtcac ttttctcttt ttctgatttg ttattatttg
     2521 taggaaaaaa gatagaaagc aaaagacttt tttcgattaa agcgctattg gatattttat
     2581 tttttgcaaa aaacttcttg aaaattttca ttttcattga ttttagtaga aaactaccga
     2641 aatgtttggt aaaatagggt cgttggttaa gaattgtctt aacaaattta gttttacaaa
     2701 acttaggagg aattttatta tgccagtagt atcaggagct gaatttttaa aagctgctcg
     2761 taaaggcggt tacgctgtcg gcggattcaa cacaaacaac ttggaatgga ctcaagcaat
     2821 ccttgaagcg gctgaagcaa aacaagcacc agtacttatc caaacatcaa tgggtgcagc
     2881 taaatacatg ggtggttaca aaatctgtaa agatttaatc actgatttag ttgaagcaat
     2941 gaacatcact gttccagttg ctatccactt agaccatggt gattacgaag ctgctttgga
     3001 atgtatcgaa ttaggctata cttcagtaat gttcgatggt tcacatctac catttgaaga
     3061 aaacttgaaa ttagctaaag acgttgttga aaaagcacat gcaaaaggta tctcagttga
     3121 atgtgaagtt ggttcaatcg gcggagaaga agacggaatc atcggtaacg gcgaattagc
     3181 tgatattgaa gaatgcaaac aaatggttgc tacaggtatc gactacttag cttgcggtat
     3241 cggtaacatc catggtcaat atccagaaaa ctggaaagga ttagcttttg accacttaca
     3301 agcaattgct gatgctgtag gtccagacat gcctttagta ttacatggcg gatcaggtat
     3361 tcctcaagat caaatcgaaa aagctatctc aatgggtatc tcaaaagtaa atgttaacac
     3421 tgaattccaa ttatcatttg ctgcagctac tcgtaaatat atcgaagctg gtaaagataa
     3481 agaaggtaaa ggattcgacc ctcgtaaatt attagctcca ggtaaagcag caatcgttga
     3541 agatgctaaa tctcatatcg actggttcgg ttcagctaac aaagcgtaag catccacata
     3601 acagatacga tatataggaa tggagaacga cttttgaagt cgctctccat tcttttttac
     3661 ttaaaacggc agaccatgct cgtttctttt ttttatctat gataaaatac aagcatatcc
     3721 atgaaaaaag attaataaac aaggtggaaa cagatgaaaa aaatcgtaat caaaggaaac
     3781 cgacctttag ctggtgaagt aacgatcagc ggtgcaaaaa atagtgcagt cgcattgatt
     3841 ccagctgcaa tcttagctga ttccccagtg atcttggaag gtgtgccgga catacaagat
     3901 gttcattcat taattgaaat tttagagatc atgggcgcaa ctgtccattt ttccaacaac
     3961 atactagaaa tcgatcctcg agaagtcgta tcgattccga tgccgcacgg aaaaatcaat
     4021 agtttgcgtg cttcttatta ttttatggga acactgcttg gtaaatttgg cgaagcggta
     4081 gtaggcctgc caggtggctg ttatttaggc cctagaccga ttgacctaca cgtcaaagga
     4141 tttgaagctc taggtgctga agtgacaaat gaacatggtg ccatgtattt acggacagaa
     4201 aataaaggat tgcgtggcaa tcggattttc atggacgtgg tttcagtcgg agcaacgatc
     4261 aatgtgatgt tagctgctgt aaaagcaaaa ggacagacag taattgaaaa cgctgcacga
     4321 gaaccggaaa tcatagatgt ggcaacttta ctgaataata tgggggcaaa agttcgagga
     4381 gcgggtacag atgtgatccg tatcgaagga gtcgaaacat tacatggctg tcggcatttc
     4441 atgattcctg accgcatcga agcaggaacc tatctagcat tagcagctgc agtaggaaat
     4501 ggtatcaaag tgaaaaatgt gatctttgaa catttggaaa gctttatagc aaaattgcag
     4561 gaaattggtg tccatatgaa aatcagcgaa gatgaaatcg aagtttaccc ctcgaaagaa
     4621 ttaaaacccg caaacatcat gacctatcct tatccaggat ttgccacaga tctgcagcag
     4681 ccacttactg cgttactatt gatgactaca ggcacttcag aaatcatcga tacgatctat
     4741 gcgaaacgtg tgaaccatgt gccggaacta gcacgaatgg gcgctgatat taccgtggaa
     4801 ggaaatatga tcatcgtgaa tggtccaaat aaacttcacg gcacagaagt cgtggcttct
     4861 gatcttcggg caggtgcttg tttagtgatt gctggactat tggcagaagg gacaacgacg
     4921 atttacaatg tagattatat cttacgcggt tatgatcata ttatcgagaa actaacagcc
     4981 ttaggggcat caattgaaat gatagaggaa gtgggagctg aatgagcgat tatttgacaa
     5041 tggcagaact agagaacaaa acgttaaaag aagtttatag ttatgccaaa gaatttaaaa
     5101 ttccttatta cagtaagatg aataaaaagg aattatctct agctgtgatt cgtgcacaag
     5161 cagagaaaca aggattttat tatatggaag gaatattgga tatcgttggt caagatggtt
     5221 acggtttttt acggccaatc aattatggac caagtgcaga agatatctat atttctgcct
     5281 cacaaatcag acgattcagt ttgagaaatg gggacaaagt ggcaggaaaa gcccgtccac
     5341 ctaaagaatc ggaacgttat tatggtttga tgcacgtgga aagcgttaat ggaaaagatc
     5401 cggaagaagc aaaagaacgt ccgcattttc cagccttaac acctctttat ccgcaaagac
     5461 agcttcaact ggaaacaaca ccgcaaagac tatctacccg aatgatcgat atcttttctc
     5521 caatcggttt tggtcagcgt ggattgatcg tcgcaccgcc aaaagcaggg aaaacttctg
     5581 tattaaaaga aatcgcaaat ggtatcaccg aaaactatcc ggaagtagaa ttaatcgttc
     5641 tactgattga tgaacgaccg gaagaagtaa cggatctaga acgaagcgtg aaaggcgacg
     5701 tggtatcttc tacatttgat ctgcagccgc aaaaccatac tcgagtttct gaattagtat
     5761 tggaacgcgc aatgcgttta gtcgaagata aacgagatgt cgttatcttg atggatagta
     5821 ttactagact tgctcgggcc tataacttag tcgttccacc aagcggacgt acattgagtg
     5881 gcgggattga cccagccgca ttgtataaac ctaagaaatt tttcggggca gcaagaaaca
     5941 tcgaagaagg tggcagtttg acgattttag cgaccgcttt agtcgataca ggaagccgta
     6001 tggatgacgt gatctatgaa gaattcaaag gaactggaaa tctagaatta caactttcac
     6061 gtgaattatc tgaacgtcgg atcttcccag ccatcgatat caaaaaatca ggtacccgaa
     6121 aagaagaatt attgatgcca agtgaagaat tagaagaaat ttggaaactt cgaaaaaata
     6181 tgactggaga ttcattagaa tatacggaac aactgatccg ctttttgcgt aaaacaaaaa
     6241 acaatcagca atttttcaaa gaatttcaag atgtctcctt tggtaaaaaa aatccgacca
     6301 gaaatctgaa aagataattg caaccttgac tgaaacatgt taagatgtta atgttgtaaa
     6361 tcgacaaatc aactctggtt cagcaaatgg agcagggcaa aggagcgaaa aaatcatgaa
     6421 agaaaacatc catccagatt accacccagt agtattcatg gattcaacaa ctggtttcaa
     6481 attcttgtca ggttcaacaa aaacttcaca agaaacagtt gaatgggaag acggtaatac
     6541 atacccagtc atccgtgtcg aagtaacttc tgactcacat ccattctaca ctggacgtca
     6601 aaaattcaca caagcagacg gacgtgtgga ccgtttcaac aaaaaatacg gtctcaaaga
     6661 cgcaaacgct gcggaataat aaaaacgctg ataactcaac gtttagcaga ctatctgata
     6721 gggatagtct gtttttttat gttttttgcc aatttaaaac ttcgtttgat gctgaaaaag
     6781 tcgtaggatc ataatgcaca aaacaatgaa aaactagtat atagttttaa ttatccactt
     6841 gataaaaaca ccttatatta acgaaatgaa gaggggttat ttcgaagtaa aaaaccattt
     6901 acctaaaatt agaagctaag ctttcctttc aaggaaggct ttttgttttg ttatagactt
     6961 gcgattgttt gtgagctaga tcgttcattt taatgactta tacacgcgga tgatgggtct
     7021 ttttcctctg ttcattcata atattgaacg gtacagctag cgatgctata ctgacctcag
     7081 ttccaagaaa acgttaacat tttttgtggg ggagaggttt tttatgaggt ttgatcaatt
     7141 gatcgcagaa gaactgattg ttttcgatcc atcgatagat tcaaaaggat cattatttga
     7201 gcgaataggg gatgtgttga aaaaaacaaa taaggtaaga aatgttcaaa aagtcgttaa
     7261 ggcactattc aaacgagagg aggagatctc cacaggaata gaagatggat ttgggattcc
     7321 ccatgcaaaa agcaaatatg tgacaaaacc agcactgtgt ttttttcata cagccaaatt
     7381 aacggactat agcgggatag atgaaatgcc aattgaatat atttttgcca tcgtagttcc
     7441 tgaaaaatca gcagatatgc atctagagat tctcagcaca ctctccagac gattgatgaa
     7501 tgcagatttt agaaagggaa taaaagaagc cactacgcca gcagatatca tcagacagat
     7561 tagtcaagaa tgaaaatagt agagtctttg tccgagtatc gatagtgaag aaaaaggagg
     7621 aaaataatca tgaaaatcgt tggtgtaact gcttgcccga ctggaattgc tcatacttat
     7681 atgtcggcag aaaaattaga agtaacagca agaaatctaa accatcaagc aaaattcgaa
     7741 acacaaggtg taaaaacaga aaatcagtta tctgaacaag aaatcaaaga ggcagatgca
     7801 atcattcttg cagtagataa agaaatcgaa ttggaccgat ttgccgggaa aaaagtaaag
     7861 cgtgtctcta caagccgagc aatcaaagaa ccgcaagtag taatagacga agcattgaga
     7921 gatattggcg tattcgttgt gtctaatgaa aaagaaccag ctgcaaatga gaaaaagccg
     7981 acaatctata atcatttcat gaatggtgtg aattacatgc ttccatttgt gatcgcaggc
     8041 ggaatcatca tcgcactgag ttttgccttt gggattacag cggctgatcc caactcagca
     8101 gactataatg tgttagcagc tgcattttca aggatcggag gagatacagc gtttgccatg
     8161 atggttcctg cgttaggcgc ggggattgct acatcgattg ctggaaaagc aggttttgcc
     8221 ccaggaattg ttgccggatt actggcctct acaggtggtt caggttttct tggcggaatg
     8281 attggtggcg tgctagcagg gtatatctgt gattttctgg caaataaaat tagcgtaaaa
     8341 aaagaatttt ctgctattta tcagctgatt gtcgtcccct ttttatcgat cttaattatt
     8401 gggcttttga tggtatttgt gattgaacaa ccgatcacat gggtgttgga tgctttgaca
     8461 aattggctga atagcttggg aaatacatcc gggctattgt tcggattact tatcgggatc
     8521 atgatggcag ctgatatggg tgggccgatc aataaatcga cttcgacttt ctcgatcggt
     8581 ttgatgtcag ctggagtaaa tgcaccgatt gctgcttgta tggctgcagg aatgacacct
     8641 cccctaggta ttgctttagc cactttatta ttcaaacata aatttaccaa agaagaacgg
     8701 acttcaggtc aatcatgctg gattttaggc gcttcgtata ttacagaagg tgctataccg
     8761 tttgcggtat ctgacccgat ccgcgtgata cctagtttga tgacaggttc agcagtcgct
     8821 gcagggattt ccatggcagt aggagttact tcaatggcac ctcatggagg gatttgggtg
     8881 atgctaattc caaatgttat gaacaattta cctatgtatg tcttggcaat tgcatcaggt
     8941 acagtcacta cagcctttat gcttggacta ttgaaaaaaa cagtgaaaga gaaaaaacaa
     9001 gacaagtgga cgaaaaatga aaatctaaca aatgaggtgg aagcgtaatg ttatatacaa
     9061 tgaaagagtt attgaaagtg gctaaagaga atcaatttgc agtaccagcc ttcaatattt
     9121 gcagctatga tatgatgaaa gcaatcatcg aagaagcaga gagattagaa gcaccagtaa
     9181 tcttagaaat tcatcctgat gaaatcgcct atttaagtga tgagtttgtt gcggcagtaa
     9241 gagcctatgc ttatacaagt aaactgccga tcgttatcca cttggatcat ggcggtacga
     9301 tagaagatgt cttacgggcg attcgaaacg gctattcttc cgtgatgatc gatgcgtctt
     9361 tgaaatcatt cgacgacaat gtacagatta cgaaaaaagt ggttgcgtta gctcatgcgg
     9421 taaatgtctc agtggaggct gagcttggaa cgattgggaa taatggttct gctgaaggag
     9481 gtgctgaaac aatcatttat acagatcctg atcaagcgaa gatttttgtg aaagaaacag
     9541 gaatcgatac acttgctgta gcaattggaa cagcgcatgg cttgtatccg aaaaacagaa
     9601 ctccaaaatt aaatatcgac ttactgaaag aattgaacca acaaatcgat cttccatttg
     9661 ttttacatgg cggttcaggt aatccggacg cagaagtcag cgaagcagta aaatacggag
     9721 ttgccaaagt gaatttgagc tcagatctta aaagcgtctt tttcaatgct ttgagagaaa
     9781 tattgagtga aaatccatcg atgtacgagc cgaatgcaat ttatccagca gctaataaaa
     9841 aggtacaaga agtggtcaaa cataaaatgg aaattttgaa cacagtaggc aaggctagtt
     9901 tatactaaaa ataaaaatgg ctctttgtca aataggactg atagagtgag ataagaacat
     9961 ctaaggtaga attccttttc tactttagat gttttttgat tcataggtaa tcagattatt
    10021 tagtaagaaa attctggtct tcattttccg aaagcttcga aagccataag cgactcgttt
    10081 caatgctttg atgtgggtat gcagattttc tattttggca tttgaataag gcaattcgag
    10141 ggctaaacga atgccggatt catagtttat aaaagctttt tttatagaat gcagttcttt
    10201 tggaacggaa ttgggcatcg ttcttacaag tttaaagaaa gtgtccgcct ctttgtcgtg
    10261 aaacgcttga agaatttctt gatagtaaaa ataggcgatt cgtaattcgt cagagatcga
    10321 aagcagacga tccaccactt cactttcggt gagatacgcc caaccaaaat tgcgccattt
    10381 ggtccatttg tcataaacaa gcttgtcctg tcgtttgatc agaagccgag acaacgcctt
    10441 taattgtcga taatatttgg cttgtgttgg atcgtttcga tcaaagcttt tcatgattcg
    10501 tacgcgaata tcctcaaatt tacgaatgag atgtttgaca atgtgaaaac gatccacaac
    10561 gagttttgcc tttggaaaac attcctttat cagagaggca taagaaacat tcatgtccgt
    10621 cacgatgacc tttaccttca atcgtgcgcg tcgttcaaat tgcataaaat aagaaacgag
    10681 agcctttttc ttccgtgaag gaagaatatc cagtaatttt ccagtatcgc catccataca
    10741 agaaaaggag tagggaccga cttgattttt cagtgaacga aattcatcca ttaataaaca
    10801 acgcggtaac caatttcttt ttacttttgt catggggatc agagaagcga gtgtccgaga
    10861 aacggtcgac caagaaacgc cgcaatgctt agcaattgtc gtcattgctt gtttttcagt
    10921 tagctcttct aaaattttat agcgcacttg tttagcgata aagcagtttc tatcgacaat
    10981 gtaggtattc gccgtaaaat aggtgtggca gccacgacag tggtagcgtt gtttcgctaa
    11041 agcaagataa gtcagttggt tcgcacattt attaagttga atgagcgatt ttttctttcc
    11101 gttcttaacg atcatgcctt catgattcat ctgtttacac aaaggacagc gacgaatttc
    11161 tttacttaac gtcgcagaaa caacaagact cttgatattc ttaatgctct tcgattccac
    11221 atttgtgatg aaaatgtttt tatctttgat tccaagtagt agtttgatat gatgaagatc
    11281 aggcatacag aacgctcctt tgattgtgtt gtggtgattc aattctaacg gagcttctgt
    11341 atgttttgtt ttttcatgct caaaagggac cgatagaatg attttctatc agtcctagaa
    11401 attatagagc caagaaaaga cggttttttt gtacg
//
LOCUS       DANKHL010000091        11311 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000091 DANKHL010000000
VERSION     DANKHL010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.094"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            226..894
                     /locus_tag="PW101_001664"
     CDS             226..894
                     /locus_tag="PW101_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDL2514064.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1324)
                     /locus_tag="PW101_001665"
     CDS             complement(1094..1324)
                     /locus_tag="PW101_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514065.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1451..2833)
                     /locus_tag="PW101_001666"
     CDS             complement(1451..2833)
                     /locus_tag="PW101_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL2514066.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="PW101_001667"
     CDS             complement(2989..4176)
                     /locus_tag="PW101_001667"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDL2514067.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="PW101_001668"
     CDS             complement(4183..5214)
                     /locus_tag="PW101_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL2514068.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5260..6684)
                     /locus_tag="PW101_001669"
     CDS             complement(5260..6684)
                     /locus_tag="PW101_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2514069.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6859..7764
                     /locus_tag="PW101_001670"
     CDS             6859..7764
                     /locus_tag="PW101_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL2514070.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="PW101_001671"
     CDS             7855..8532
                     /locus_tag="PW101_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL2514071.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="PW101_001672"
     CDS             8529..8699
                     /locus_tag="PW101_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514072.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="PW101_001673"
     CDS             8813..9709
                     /locus_tag="PW101_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2514073.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="PW101_001674"
     CDS             complement(9764..11134)
                     /locus_tag="PW101_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2514074.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
BASE COUNT         3600 a         2218 c         2207 g         3286 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgcaag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt taatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt c
//
LOCUS       DANKHL010000092        11278 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000092 DANKHL010000000
VERSION     DANKHL010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.095"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            206..1687
                     /locus_tag="PW101_001675"
     CDS             206..1687
                     /locus_tag="PW101_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2514075.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(1790..2497)
                     /locus_tag="PW101_001676"
     CDS             complement(1790..2497)
                     /locus_tag="PW101_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDL2514076.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(2576..2914)
                     /locus_tag="PW101_001677"
     CDS             complement(2576..2914)
                     /locus_tag="PW101_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDL2514077.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(3025..3987)
                     /locus_tag="PW101_001678"
     CDS             complement(3025..3987)
                     /locus_tag="PW101_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDL2514078.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(4002..5333)
                     /locus_tag="PW101_001679"
     CDS             complement(4002..5333)
                     /locus_tag="PW101_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2514079.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(5341..6021)
                     /locus_tag="PW101_001680"
     CDS             complement(5341..6021)
                     /locus_tag="PW101_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDL2514080.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(6240..6719)
                     /locus_tag="PW101_001681"
     CDS             complement(6240..6719)
                     /locus_tag="PW101_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HDL2514081.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(6846..8525)
                     /locus_tag="PW101_001682"
     CDS             complement(6846..8525)
                     /locus_tag="PW101_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL2514082.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            9073..10575
                     /locus_tag="PW101_001683"
     CDS             9073..10575
                     /locus_tag="PW101_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2514083.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            10588..11251
                     /locus_tag="PW101_001684"
                     /pseudo
     CDS             10588..11251
                     /locus_tag="PW101_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT         3407 a         2351 c         1954 g         3566 t
ORIGIN      
        1 atcaattttt ccgccttaaa aacaagaata tcttgacaaa ataccctagg aaataaacaa
       61 tagaataaac aaggtaacaa aaaaattaaa taacgttata taaaaaagaa ggcgacactc
      121 tgtgagaata acacagactg aaatgacttt ccactcacag tttttgtcta actatataca
      181 aaaacagaat aaaaaggagg tatttatgct aattgaagca agaaaaaaag agttacttgt
      241 tctacaaatg gtcattcaaa aaaataattg ttctttacaa gaattatcca atgacttaca
      301 tattccaaaa cggacaatca aagaactgat ccgaaaaata aatgtatcac tcaagcaatt
      361 tttattgatt gatgaattta tttattctaa tcataaagga gagatccaaa ccaaagaaac
      421 cgatcaaact aaaaagttag ctgttttttc taagttaaaa ttatgctacc taaaagaatc
      481 caatcgtttt aactatttga tgttactaat caattatcca caaactgctg taccaaaaaa
      541 atatttactt gaacaacttt atatatcgcc ctcctatctc gaaaaactga caaaacagct
      601 caatcattcc ttaataaaat tccatttacg gatcctatct gtaaaaaatt gctgtactct
      661 tgaaggaaat gagttatcta tccgtctcta tctttacttt tttctttcag atacatttgg
      721 aggtaacgat tggccatttt cctcacttga attaaatgca ctaaaaatga acagtacaga
      781 tgagcaaata gaaaaaattt caaaacaaga aaaaaatatc tacttgctgt taactttatt
      841 catattacga aacaaacaac aggcatattt tccaaaaact tcttctgaaa tcactgaatt
      901 gatggactta ttgcagcata cacatgatta ttcagaatac tttacttctt tttcagtgag
      961 tcgcccttgt gaagagacag aaacagagag agcctacttt aattattttt tctattgttt
     1021 ttttccaaat caaatctctt ttcgacaaaa acaggagatt ggttttttat tttccacaag
     1081 caaaaacctt atttgtcgac aagtcggtca attgatgact tacttaactc aacagtttcc
     1141 tctcaaccat ttagaaaata aaataccaat ttatttttat tttcttgtgt tggcagttgt
     1201 ttttttcgaa ctgttgggtg aatccagcag tagtttttct acactttgct ttccagaagc
     1261 cacgtccaat cattctagtt cttttataca ggaaaagaaa ttaagagaaa aaatcgatac
     1321 aattttttca aaaaatgggg aaagaaacca aatcagtcaa taccttagtc aattgatcca
     1381 acacttacta caaacagaaa cgaaagagca gttactcatc catttgcaga taaccaaaca
     1441 gtttacagga aattatcatc ttcaaagcca tttgcagcag ttatttcgca aagatgctgt
     1501 taaaattacc gcatattcag aacatgctga tttgatcatt acagatcagc atgagaaaat
     1561 ttcagatgaa acggagactt tctatctgga tacaacgcac aatacagtgt tatttggcaa
     1621 gttaataaaa gcggttcatg aactgtatat gaaaaaaggt tatgagaaca aaacaaagag
     1681 gatgtgacaa aattcttgtc acatcctctt attccaaata aatggtattc aatcagctaa
     1741 ctctcggtat tagctcagat caaagcgctg atttcacaac ttctttattt taatctcttc
     1801 ggttgccgcc gaacaataag atcaagcgca acagctgcag gaatgtcgaa agcgctgctg
     1861 caacataagt cagtgctgct gcaaataata cgtgtcttgc catcggtact tcttcttccg
     1921 tcagtagtcc gccttcactc aaaatttgga ttgctcgccg agacgcgtta aattcgactg
     1981 gtaatgtcac caattggaaa agtaatgcta aagaaaaagc gaaaatcccg atattgatta
     2041 acgtagagtt ccagctaagc aatacaccta ccataatcaa ggggaacgaa atcatcgaac
     2101 caaagttagc tgctggaaca agtgctgcac gaatcttcaa tgggacataa cccgaagcat
     2161 cttgtaccgc atgtccgcat tcatgagctg ctacacctat cgcagcaact gaagtagact
     2221 gtgcagttgc ttgagacaaa ctcaacatct tattacctga attgtagtta tctgtgagat
     2281 ctccggcaat ttgttgaaca ccgacattat ttatctgttg actttgtaat atatattgtg
     2341 ctgcttgtgt accagtcaca ttgtttttgc ttctcacttg atcgtatttt cggaaagtac
     2401 tattgacgta cgctgacgca attccggaaa taactaatcc aagaatgacc aaaagaaatg
     2461 tcggatctaa aaaaccatac attccataac catacatttt cgtttcactc cttttcgttt
     2521 actttatttt atcattagta gtaggaaaag aatgtgaaaa gaccttgaat attctttaat
     2581 tctttttcac aaattccgat ttcagtttca tcggtccaaa tccttctacc ttgcaatcga
     2641 tattatgatc gccttctact aaacggatgt tcttgacttt tgttccttgt ttaatggcac
     2701 ctgacgcacc ttttactttc aagtctttaa tcactgtcac gctatcgccc tctaacagac
     2761 ggtttccatt tgcatctaat accactaatc cgtcttctat gacagtttct tgttcttctt
     2821 tgttccattc gttcccgcat tctgggcaaa tcaacaagcc tcgatcttca tatgtgtact
     2881 ctgagccaca ttctgggcaa tttggtaatg tcatcttctt tctcctttaa aatctttagt
     2941 gagcactatt gtaacggaaa aagaagatct tgaaaagata catttgtgat ttttactaca
     3001 aaaggcacgt tttcgaacga agtatcattc tgtttttctg ctcttatacg attggcatat
     3061 atcttggatc tctttataga tttcctctac ttctgctaaa tcttttgcat tcgcaccact
     3121 tgctaatgga tggcctcctc catgatggcg tttagccaat tcattgatga tcggcccctt
     3181 agaacgtaaa cgtacgcggt aataccctga aggctgctga acaaaaatcc cccaagccag
     3241 cacttcgtca atcactccag gaagtgaaac gatcgaagct gtctctgaat catcgattcc
     3301 atatttatca agaatttctt gcggaaggat tattcttcct gcaccatttt catccacctg
     3361 gatattttga taaagataac cggaaagttt agcgactttc atcggcattt gttcaatctc
     3421 tctgttcaaa gcacttgcat caaatggata atttctcaat atcgaagcaa tctctaatgt
     3481 acgggacgtc gtggagggat acaaaaagcg ccctgtatct ccgacgattc ctgcataaag
     3541 taaccgtgca gccttaacgt tcatctttaa agattcagga aacatcaaag caaattctgc
     3601 aatcatctca ctacagctac ttgcttttgt atttacccac acaagatctc cataagggtc
     3661 atcattggga tgatggtcga ttttgatcag tttttctgcc aactgatatc tttgatcact
     3721 gattctgggt gaattagctg tatcagtcac gatgactagc gctccttggt agaagacatc
     3781 ttcaacttct tgcatttctg ctaaaaagcg aagcccttca atcgtttcac ctacttgacg
     3841 tatttctttt tctgggaaac tttcccgtaa tagttcggct aatcctactt gtgatcctaa
     3901 tgcatcagga tcagggcgca aatgcctgtg gataatgatt ttatcgtatt tttttattgt
     3961 atctactatt tcttgctgta ctgtcatttc actcctgctt tctaagaacg ttccattact
     4021 tgacaaacaa cgattgcttt ggaaacgatg gtgttttcaa taaacacttc gatatccagt
     4081 ttagcggaac gtctgccgat ttccaataca cgtggacgaa tgtctaattc gctttccaat
     4141 tggatcagcc gcaaataatg taggttcact tgttcaatca agatatttcg tttttggttc
     4201 atcaccattg ttttttgtgc gacatcagaa atgatttcgc taagtacgcc aaaagaaatg
     4261 gttccaacgc cattgaccat ttgtggtgct actacgaatt tgaacttagg gttttccaaa
     4321 cgatttcctt cacggtcagt atcaatcgta tggatatttc cagatatctg atccgaaatc
     4381 gtatctgcaa tctgcggttg tcgctggaca agctgcatcg ctttcattac atcttgccgg
     4441 gtaatcaagc cttccaatgt caagtcatcg gaaacgaccg gcatgacttc tagcccatcc
     4501 cagatcattt gatggctaac agaggcaata ctcattgttt tctttacact gattggttct
     4561 ttcgtcatca cccgatcgat gatctggttt ggcgatttcc ctaaaacatc ttttgccgta
     4621 acaatcccca tcaaacgatg atggcggttg acgactggaa aacgcgaatg ctgcgtttct
     4681 tctgacaaac gctgataatc agaaatcgta tctgtctgct gcaaataatg agtcttttca
     4741 gctggcatat agatatcact gacaagcatg atatcttttt tgatcaattg gtcgcttaac
     4801 gctcggttga tcatggttgc gactgtaaat gtatcataag aggtgcgtaa gatcggcatt
     4861 tcaagatcat cagctagctt tgcaatctca gcagtcgtat tgaatcctcc tgtgatcaat
     4921 actgcggcgc catcttccaa tgctaatttt tggacttccg cccggttgcc gacgatcatt
     4981 agtgatcctg gcgtaatata gcgttccatg gcatgtttag tcatcgcgcc gatgacgaat
     5041 ttattcaaga ccttgtctaa tcctgccgcc ccgcctaaaa cgtccccttc aatgatcctg
     5101 acgacctcgc caaatgtcaa acgttcaata tgttttttta atttgcgttc gatacggatg
     5161 gtccctactc gttgaattgt tgaaactagt ccgacatttt ctgcatcttt gattgcacgg
     5221 tacgctgttc cttcactgac attcaattct tttgcgatgc ttcttacaga aattcgattg
     5281 ccaacaggta aactttcaat atattctaaa atcaaatcat gttttgtcgc cattttctgc
     5341 ctacacttcc agcgtttccg agacgctcat tatttttcct tcactatctt ccagtaactc
     5401 tacaaaagat tcgggatctt gttcaatgac agggaaagta ttgtaatgga ttggaactac
     5461 tttcttggca cgaattgttt gcgcagcctt tgctgcatct tctggcccca tcgtatagtt
     5521 atcgccaatc ggcaagaaag cgacatcaat atcgaattct tctgcaagca atcctagatc
     5581 actgaaataa gcagtatccc ctgcatgata aatcgttttt ccttctgctt gaataataat
     5641 tccgctaggc tcgcccatat actgaacgat accgtctact tcatagcttg aactatgctg
     5701 tgcatgtacg aattttacca cgccgaatgg aaacgcatgt tttccaccga tattcatacc
     5761 gaatgaatct actcctctgc tgttcgcata atcagcaatc tcaacaatac tgataattgt
     5821 ggcttgattt tcttttgcaa taggaatcat atctcctatg tggtcactat gaccatgagt
     5881 aatcagcaac caatctgtct ttacttcttc tgcagttaag tctgtcaatg gatttcctgt
     5941 aataaacggg tcaatcatca cgcgttggcc attttccatt tcaattgtta aacaggaatg
     6001 tccatgatac gtaattttca tattttacac ctcgcgtttt ctctgtatct tttttattaa
     6061 aaacgatttc atcacttgca catcaatgaa aaaatatagc tgctaaatat attttcattg
     6121 gattgatagt acagtatgta caaagcaatt gtaacagata atacaggatg caccaagtac
     6181 caagcagata taatctcaag tttgaggtct ctgctctgct tagcttctct tctcgtccct
     6241 taactaatta aatcagcgta aataggatgg acgatctttt ctaaatcggc tacttttatc
     6301 cgaaggcttc tcccgatttc accgcttgac acaattactt cttctttttc ttttaaagaa
     6361 ctgtctgcta aaaaacaata atcaggatgt gcgagtttta ttccaatagg ggtgtttgct
     6421 ccgtggggat atcctgtatg cattaataca aaatccattc caggaaaacc aactttacga
     6481 tctttcgtca cttgtctcgt ttttttgtaa tctaagtgat cgttcaaagg aatcaatgcg
     6541 atgatcaccc cagttttgtt tcctttcact acgagagttt tacatatcaa agctggatca
     6601 atccctcttt tttgtagttc ttctgtgaaa gattcttcta ccacatgact aaaatcaagt
     6661 ggttgatagc tgatcttttg ttcttctaaa taacgttcga cttttgtttg actcatcatt
     6721 gattcactcc ctttattcta tcgtttatta taaagcgagg gatgctgtat tcaaaaatta
     6781 tttgtttttc gcaaaaaaag agcaagacga gcactctata gagaggttca tcttactctt
     6841 tttgtttatt ttttatttcg acgttgagta gcttccataa tgactggcaa gatcactggg
     6901 cggcgttttg tttgttcaaa taagtaacgg cctaattgat ctcggatgtc ttgctttaat
     6961 ttgctccact caaaatcatt gctttctaaa tgtttctcaa cgattgtttc aatcatattg
     7021 ctgctttctt tcatcagatc acggctagct tttacgtaaa cgaatccccg tgaagtaatt
     7081 tgaggttttg aaatgatttt cttttctcgc cgactgatag tgacaaccgc tacgaaaatc
     7141 ccgtcttctg acaagatttt tcgatcacgt aaaacaatgt ttccgatatc acctatgcct
     7201 aatccatcaa tcattacatt ttctgcactt gtagttcctg caacactcat acgcttgttc
     7261 ttatattcca gcacgtctcc gcgtccagta atatagatat ttttgtatgg catccctact
     7321 gcatgagcca aatctgcatg agcagctaat tcacgatact ctccttgaac tggcacaaaa
     7381 tatgttggtt tcatcaaatt gatcattaat tgcaagtcat taggattggc atggccagaa
     7441 acgcgcatat tctcagagat caacttcact gttccaccag ctcgataaat gatatcttcc
     7501 gttttcgcca cgatggtttc catcgcgatg cttggcgtag tagtgatata aaccaaatct
     7561 ccttcttcaa tcttgacatt acggtgtgta ccgtttgcca ttttttgcag agacttgatg
     7621 ggttcaccca ttcggccagt ttctaaaatg atcagttctt ctggatcata gtttttcatg
     7681 tctttcaacg taacaagcag atcttcactt ggtaattgaa gtttttctaa acgcatcgct
     7741 gtgcgaataa ttcgttcgaa atcttgacca gtcaagacaa cttttcgttg tgctttttct
     7801 gctgcatcca atacttgttg cactcgttgc aaattacttg ctacacaagc gacgatgata
     7861 cgcccttccc aatatttgat cgtatcaaat acttcatctg ctatttgggc ttcagaagct
     7921 acaggctgag gattttctgc atttgccgat tcacttagta aggctaagac tccttctttg
     7981 ccgatttccg ccaatcgtgc ataatctgtt tgatacattg ggatagccgt ttggtcaaat
     8041 ttaaaatctc ccgtataaac gatactacct tcttttgttt tcagattgat ccccactgaa
     8101 tctggtatcg tatgagtggt acggaaaaag ctcactactg tttcaccaaa gtctatttct
     8161 gtatgctcat ctaccacatg gaaatcctta aatcctttgg ctgcttcatt acgactgaca
     8221 ttcaattttg ctaactcgat cgtcaactct gtcccgaata ctggtacaga aatttttgac
     8281 aacaagtaag gaagtgcccc gatcgcatct gcatgtccat gtgttaaaaa gacaccagct
     8341 acacgatcaa tgttttcttc taaatacgta aaatcaggga ttacgacgtc gatccccaac
     8401 aactcatttt caggatattt tagcccacaa tctaaaataa aaatatcctc gcccacttct
     8461 gcgacataca tgttttttcc attttcacga acgccgccta atgggataat tttaatggta
     8521 ctcacaaact tcacctcttc ttatgacatc caagtttaaa gatgtcgatt ttcttcttta
     8581 tctaaatata tgagccattc caatgaagca tatgcaaaat cactatttct caacttatgg
     8641 agaatcagca ggaaaattac cagcacatcc gcgttctgac agttggatgg gcattttatt
     8701 ttggatatac ttcattaaca aaatggatct tttgcgaaac gctatagcca gaatacttat
     8761 ataagtattc agctgttccc cattattata caacaaataa actaaatcgc tagtatcagc
     8821 aattgttaga acaactatgc acgtaaaaat ccctctgtag catctaggat ttcttttgct
     8881 attcctcttt catttcttct tcgtcggttt gaaacatact tttttcattt taccacattt
     8941 tttgcaataa ctatagtgac tttttatata tacagaacct catgttgatg gtgtgcaaac
     9001 agcataagat ttaagctagc tttaagatag gttgtctatt atcaaatgca tctactagga
     9061 gggatgcaaa atatgaattt tatgaaacgt gcatggaaaa gtacaaaagt aaaatgggga
     9121 cgttcattgt tattgtttgc cgtgttcaca gccattctta tttttgtgct agctggcctc
     9181 acgattcgaa gcgctgcaga agaagcagcg aatcaagcac aaaaagaagt tggagcaaca
     9241 gtgactttaa gtgccaatcg agaagcttca tttaaaaaac aggaacaaac ttcttcaagc
     9301 ggttctacca ctgaagaacg tccagatcca ggcagttttt ctcttacacc agtttcctta
     9361 acggacgcgg aaaaaatagc tgaattagat aatgtaaaga gttattcctt tgaaagttcc
     9421 gcctcagcta tggcaaaaga tggaataact gccatcacta gttctgattc aagtgaagac
     9481 agttcagaaa ctactgatgc aggcggtgaa cctgctggag gaatgggcgg gaatccgcaa
     9541 atgactcaag cagactttca aatcagtggt gtgtcagcaa ctgcacaaaa tagtaatttt
     9601 tctgatggaa cagcaaaaat cacggatggt gaaggaatag atgacagcga caaaggcaca
     9661 aacaatgtag tcattgattc tactctagca gaaacaaatg acctggcagt cggagatact
     9721 ttcacaatca cgagtactga agatgaagat accacttatg aaatgacgat caaaggaatt
     9781 tatgaatcca gcgaaacaag cggcagtatg ggtatgaaat tcaacttcat gaatccgtcg
     9841 aatacacttt acacttctta cacttttgca aatgaactaa atgggacatc tgacgataac
     9901 acgatagatt ctgctattta cactctatct gatccagata aaatggatga gtttctcaaa
     9961 gaagctgaga agctgatcga cacagataca tttagcttac aatcaaatga ttccatgtat
    10021 cagtcaatgt tggaaccttt aaacaacgtg gcaagttttt ctaaaaatgt tgttttacta
    10081 gtggctgtag ccggtattat catacttact ctgatcatta tgattaccat tcgggagcgt
    10141 cggcatgagt taggcgtctt gctatcttta ggagaatcca gaagtaaaat cattctccaa
    10201 ttgtttaccg aagtggctat ttgtatgatt cttgcattag gagttgcttc gcttagcgga
    10261 aacgtcgtcg cgaatgccgt gggacagcag ctacttgacc aacagacaga aaccacgaca
    10321 caaaaccagc aagctcctgg cggtggacaa atgccaggta acagacaaga acaaggcggt
    10381 caacgcgatg gtcaacaagg aaccacgaat aatccatttg ctgtttctga tcaagtcagc
    10441 gaattggaaa tcaatgtcaa acctgcacaa ttgggactat tagccggagt agcatttgga
    10501 atcagtctgt tttctgtctt tttagcttct atagggatat taagattaaa cccaagaaaa
    10561 atcctattaa actaaaggag gaatttgatg ttacggacag aacaattggg attttggtat
    10621 caagatccgc aagatgcgct ttataaagat gtagaactga atttcgaagc tggaaaaatg
    10681 tacgctattt taggagcaag tggttctgga aaaacgactt ttctttcgct tatttctgga
    10741 ctagatgcac caaaagaagg aaaggtatac tatcaagatc gttcactgaa caaaatcggg
    10801 ttaagaagtt atcgtaaaaa tgatgtctcg attattttcc aagcttacaa cttgcttcct
    10861 tatttatcgg ctctagacaa cgtcatagca gcaatggaaa tcactaagac caagcaagag
    10921 aattatcgac aagttgcttt agatcgactg gctcgtgtag gaattgatga aatattagca
    10981 aaaaaaacag gtgaccaaac tttcgggagg tcagcagcag cgagtcgcta tcgttcgtgc
    11041 tatctgctgt gaacatcaac taatcgtagc ggatgagcct actggaaatc tagatgaaac
    11101 aacttcacaa gatatcgtca aacttttcca agaaatcgcc catgaacaaa atcgctgtat
    11161 tatcatcgtg actcatgaga ttgaagtagc aaaaagctgt gatgaagtct ttgaactaaa
    11221 aaataaaaaa tttcaaaaaa tcgaagttta aaaaacagga taatgatcaa ataaaaaa
//
LOCUS       DANKHL010000093        11229 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000093 DANKHL010000000
VERSION     DANKHL010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.096"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            6..305
                     /locus_tag="PW101_001685"
     CDS             6..305
                     /locus_tag="PW101_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2514084.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="PW101_001686"
     CDS             378..1739
                     /locus_tag="PW101_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDL2514085.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="PW101_001687"
     CDS             1761..2021
                     /locus_tag="PW101_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514086.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="PW101_001688"
     CDS             2035..2787
                     /locus_tag="PW101_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDL2514087.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="PW101_001689"
     CDS             complement(2881..3486)
                     /locus_tag="PW101_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2514088.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="PW101_001690"
     CDS             complement(3539..4513)
                     /locus_tag="PW101_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDL2514089.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="PW101_001691"
     CDS             complement(4891..5610)
                     /locus_tag="PW101_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2514090.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="PW101_001692"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="PW101_001692"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL2514091.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="PW101_001693"
     CDS             7161..8558
                     /locus_tag="PW101_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2514092.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="PW101_001694"
     CDS             8639..9079
                     /locus_tag="PW101_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2514093.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="PW101_001695"
     CDS             9227..9553
                     /locus_tag="PW101_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2514094.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="PW101_001696"
     CDS             9554..10303
                     /locus_tag="PW101_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514095.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="PW101_001697"
     CDS             complement(10383..10838)
                     /locus_tag="PW101_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL2514096.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3560 a         1960 c         2303 g         3406 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttatta
//
LOCUS       DANKHL010000094        11022 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000094 DANKHL010000000
VERSION     DANKHL010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.097"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(317..487)
                     /locus_tag="PW101_001698"
     CDS             complement(317..487)
                     /locus_tag="PW101_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514097.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(487..786)
                     /locus_tag="PW101_001699"
     CDS             complement(487..786)
                     /locus_tag="PW101_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514098.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            complement(891..1094)
                     /locus_tag="PW101_001700"
     CDS             complement(891..1094)
                     /locus_tag="PW101_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2514099.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(1109..1855)
                     /locus_tag="PW101_001701"
     CDS             complement(1109..1855)
                     /locus_tag="PW101_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDL2514100.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            1933..2241
                     /locus_tag="PW101_001702"
     CDS             1933..2241
                     /locus_tag="PW101_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514101.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            complement(2212..2469)
                     /locus_tag="PW101_001703"
     CDS             complement(2212..2469)
                     /locus_tag="PW101_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HDL2514102.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(2767..2907)
                     /locus_tag="PW101_001704"
     CDS             complement(2767..2907)
                     /locus_tag="PW101_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514103.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            3201..3521
                     /locus_tag="PW101_001705"
     CDS             3201..3521
                     /locus_tag="PW101_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2514104.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            3539..3970
                     /locus_tag="PW101_001706"
     CDS             3539..3970
                     /locus_tag="PW101_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL2514105.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            4097..4681
                     /locus_tag="PW101_001707"
     CDS             4097..4681
                     /locus_tag="PW101_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDL2514106.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            4783..5931
                     /locus_tag="PW101_001708"
     CDS             4783..5931
                     /locus_tag="PW101_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2514107.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALENYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKMIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRKLSMDKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYGEGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLSNRAKELIES
                     VIADNILAGRPTNPDQYIFISTNGTPLTLHSFNAVLHKVEEELELEKNLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKKAKQQVIDKLNRL"
     gene            complement(6020..7645)
                     /gene="groL"
                     /locus_tag="PW101_001709"
     CDS             complement(6020..7645)
                     /gene="groL"
                     /locus_tag="PW101_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDL2514108.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(7696..7980)
                     /gene="groES"
                     /locus_tag="PW101_001710"
     CDS             complement(7696..7980)
                     /gene="groES"
                     /locus_tag="PW101_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDL2514109.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            8205..8867
                     /locus_tag="PW101_001711"
     CDS             8205..8867
                     /locus_tag="PW101_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2514110.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(8943..10235)
                     /locus_tag="PW101_001712"
     CDS             complement(8943..10235)
                     /locus_tag="PW101_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2514111.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            10405..>11022
                     /locus_tag="PW101_001713"
     CDS             10405..>11022
                     /locus_tag="PW101_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDL2514112.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISV"
BASE COUNT         3437 a         2114 c         1900 g         3571 t
ORIGIN      
        1 ttttgtctaa tggtgcttaa tttaaaaatg tttataattg aaaaatggat agttattatc
       61 gcaaagattt tctcatcgat atcaattatc attccaatga cattgacgtt ttctaaagag
      121 caatttaacg tagctactac attcgtatct tttatagctc tattttcgtt tgctgttcct
      181 aaaacttttc aaagcaaatc tagcaacgca aaggttaatg tagaaaacac tgatagaaaa
      241 aatgatgaaa gttattaaaa aaaataattc tttcatttat tgtcaaacct ttcttataga
      301 cattagactt ttttagtcag tccctcccga ctggcttttt tgttttgtac tcagcttcat
      361 ccagccctat aaaaatccaa accatgtaca caatcgtacc tatcaacgct tgtctgcttc
      421 cccagaggcc taaagcgtag atgattagcg gtgcgctgaa tactagtgct ctgttgaatt
      481 tgcccattaa cttgcctcct tcaaaacatc tgaaggcgat aaacttttta cacgatattt
      541 gtttgcgtct ttccacttta aaaaccaaat aaaagtatgg taatgaataa atgtggttga
      601 gtgacctgga cgaagtattc cttctgcaaa ttcaggaatt gtttccattt ccttgcagta
      661 ttcaactaaa gtagtttttg acatgccgtg gaacttctcc aagattaaac tttgtcggta
      721 ccagtcgtct ggttttactg ccttctcagc agcttctatc agctctgaaa gtgttggttt
      781 tttcacttat tatccctcct aaaaattatc gttccatact cgatgtttaa catttcttga
      841 agctctgatt ttgtaagaat gattttatct tccaacgact ttacctccct ctacaattcg
      901 tacatagtga taatcgaatc tataattctg tttgcttctg cagaagtctt tttaccgttt
      961 aaaattaaag ataagtagct tttgctaatt ccaaatcttt cagcaagcat ggtgtaagtt
     1021 aagaactttg aactttcgac atattctttg attttttctc tatctcgttg agtgatttct
     1081 gcaatgtcag tcatactaaa actcctttct aaccaatttc ctctaaatcc atttgagggt
     1141 aatagccctc tgcgattaat aagttgtaaa taaacactcg ccctttctgt gtccatttgg
     1201 tattcattac aactttagtg ccgccatcgg ctttcggtat ctcacttgtg tgcgattttg
     1261 tgtatccttg gttcatatgt tttcggtaaa gtatccattg tccgctaact ttatgctgaa
     1321 cacctaactc gtttagcaat ttgtttagag caattgccga cattccgtaa tcagctgcaa
     1381 tttgagaagt agctaccgta tctgtcgatg aaagaatcgt atctaagtag ctaatctttg
     1441 gttcgtactc ggcaatttgt tgttcgagta attggttctt ttcttctaaa tccgctgcta
     1501 aacgcaatgc ttgtgcaaaa ctttgaggta cgtttgaata gctacctgtt tttctaattg
     1561 ttggaaggac ttcactcgtt acccaacgtt taaatttttt ggcagaggga agttttgatt
     1621 ttaagattaa actgtaaagg cctgactcgt taatcaaaac aactgctgtt ccattgacgg
     1681 tgaacgaatc gttctgcgtc ttatcctcca agtccacatg atctcgaata gctttttgcg
     1741 gattcgaata tcctagaacg tctgctacat cttttcctac aaaatatggt tcatcattta
     1801 ctaaaatagt tcgaacttcg ttttgctcga aattaaaaat ttgtggtgta ttcatatttc
     1861 tcattccttt ctttggtata atttccttat cagtgtgaca ggctgaaata attgttaagg
     1921 aggtgaaaat ttatgaaacc agaatttgta attactatgc ttgatggaga aaaaatcaaa
     1981 atatttgccg atactttggt cgttggatac gatgatgcgt caaatatcga agaaaaagat
     2041 ggaatgttct attcttctca aatttacgtg aataccttgc aaggtgatat tggggtatct
     2101 actcttgcaa catcaagacc gcttgtaggt cttatcggct ttttgtcaaa agcaacattc
     2161 ttttctctcg gcgatgatat agattcggat gaaactgtca tctataaaac atcagctgtt
     2221 aaaagtatcg gtttcaaata gtaattcttg ctcgttgata gcggaagcca acttttcttc
     2281 caacacatcg gcgagctttc tttttgttgg attcatttca aagttgatag tcttgagaag
     2341 catttttgca tccttaactt tttgtccttg caattccttg atgattttat cggcttggct
     2401 atcaattcca tgtgttagat catctatcat tttttgtacc ttgattgttt ggctcatttc
     2461 cttgttcatt gtttagcctc ctttaataaa tgattttttg tattttttaa tttccataaa
     2521 ttcctcaaat tgctctttgg tattattacc acgaccatac atattgtgaa attctttatg
     2581 gcaactgaca caaagtgtaa cgccgtttga aatcgctgcg gatttagttg ttgcatggct
     2641 aaatcacgaa tcacaagtat ctactaaagg cgaatcaatt tcgccaaaag aaattgttca
     2701 aacttattta gatattcatt atgctgttat ctatggtcag cttccagaag accgtaaaaa
     2761 cgatgactaa acgtgagcta aaatctctgc tatggctgca accatggcag agtcttcttt
     2821 atctaccgcg tgttccatcg ccacttttgc ttgtttcagt atgtctaatt tcaaatcttc
     2881 gatttttgcc gagactgttt tttccatatt cttctctcct ttcttttata ttcgtaaaca
     2941 aatttaacaa ctttttaaaa aactgcgttg actaaattaa catacagtgt taaaatcaga
     3001 acatagttaa ataagacata taaacattga ttttaaaagc tttcttggcg gttggcattt
     3061 attaatcaat agtgtttttt gttgtctttt tagttgttaa acttgtttac aagaatgata
     3121 ttaacacctt ttgttaaaat agtcaacgaa ttttaacaaa taaagttaaa tattttttct
     3181 tgaacgaaag gaatattgat atgataacgt ttgagagaat aaaagagtta gcgaaaaaac
     3241 agggaaaatc attaaataaa gttgaagaag acctaggcta tggtaaaaac gtcttatata
     3301 gacttaagaa ctcaaacccc tctacagaac gtttgcaaga aattgctgac tatttcgatg
     3361 tctctgtaga ctatcttctg gggcgcgaag aaaaagaaac ccctaaacat gtggatttat
     3421 cagaaaacga tactgttttt tcttttgatg gaaaagaaat atctaaggag acaatgcgta
     3481 aagcgattgc aatcgctaaa gctttagagg aaaatgaata gttggagtga tgggttgtat
     3541 gtatttgaaa ttgaaagaaa tgttgagcga atacaatttg aaagtagttt acatggaaat
     3601 gaaagagcct ggcttctatt atccgaagcc tagaatcatt tttttgaatg aaaatctata
     3661 tggcgaaact gcagaagcct ttcatttatc tcatgaactt gcacatttca gcgcctctca
     3721 ttttgaattt tcagttttgt acgatacttc tacaactttt cactcaaaat ttgaaacaga
     3781 agcagataaa atagctattc taattttatt aaatatctat attgagaacg aactgactga
     3841 tgaatctcag tttaacctcg aaaaatttat ggaatattat tctattcaga ataagctcag
     3901 atacacttgt tatgctgtct gccaatgcta ttttaagaaa aaatattctt acgcaaggca
     3961 atatgtatga atacatcaag aattattaaa aaaaccgatt tcagcaaaac attaaaaaag
     4021 cccgtgctgg cacacggact catacctcat ttctgagatc gcaaatatat tataacaaga
     4081 agtgaggaat atttaaatgg caaaaaaagt tatgggtcaa gacggaaaaa tgtataaagt
     4141 tagtaaacct ttttacaaaa aagtatggtt ttgggtatta gcagtaattt taattataat
     4201 tattggttct gctttaaatg gtggatcaga tagtaataaa gcaagtgata atggtggcga
     4261 aaaagtaact aaatcttcaa cctctgcttc atcttctaaa gaagaaaaaa gcgatacctt
     4321 ctataagatt ggtgacactg taaaagttgg tgatgctgaa tatacactaa atagtgtaga
     4381 actaactgat gaaagaaatc aatttgaaga aaaccaacct gcacaagtag taaaaattac
     4441 ttataccgta aaaaatgatg gcgattcaga tatccctgta ggtacagatg tggaagtata
     4501 tggaccagat gacaaaaaat cagagacata tgccaacgaa aatacaatgg gatctgttgc
     4561 tccaggaaaa caaatggatg taactgctca tttcacgtta aaccaaaagg gagaaataga
     4621 aatccacttc tctcctttag tatcatttga aaaagcagct atttttaaag caactgtata
     4681 ataaataaaa aacacgcccc accgtccaga gtaagagcgt gcttttagaa aacaaaccta
     4741 tataggtcta tttgttatgc ctattatagc aaatgatagg agatgttaaa agtgtgggtt
     4801 gaacaaacca aagatggcca attcaagttt attgaaagat atgtagatcc ttatacagaa
     4861 aaaacacgaa aaaaatctac aacacttaca agtaattcac cacaagcgtg gaaaaaagct
     4921 cagaaaattc tagataaaaa aattaaagaa gcactcgaaa attacaataa atcagatatc
     4981 acttttggtg agctgtataa agaatggtat gaatattata agcagcatgt taaacgtact
     5041 agctatttga aggttccaat gatgatgaaa catgtttcta agcacataag cgatgataca
     5101 atcgttagaa acattgatga gacactcatt aataagatga ttgaagatat gtacactttt
     5161 ggtgatctct cactgaacta cacaaagcaa acaaaaacaa ctctatccgt tatgctaaac
     5221 tatgcaattg atagaaaata cattcaaaga aaccctgctc tagcagttaa aatccatcct
     5281 aaaaaagtgg aagaagaaaa aagaaagctt tctatggata aaaaatatct ggagaaagaa
     5341 gaaattgatc aaatactgaa acagttatat tccaatcctc gcagaaaact acacggcata
     5401 atagctgaat ttctatattt gactggtttg agatatgggg aattgctggc tttgcaaatg
     5461 aaggactatg gagagggtaa aatttccatt aatgggacct tagattacac atctgtaaaa
     5521 atggataacg ccataaaaac aactccaaaa aatacttact cacaacgtga agtacaatta
     5581 tccaatcgtg caaaagaatt gattgaaagt gtgatagctg acaatattct tgcaggtaga
     5641 cccaccaatc ccgatcaata tatatttata tctacaaatg gcactccgct tacgctgcac
     5701 tcatttaacg ctgtactcca taaagtagaa gaagagttgg aattagaaaa aaatctatca
     5761 tcgcatatat ttagacatag ccatgtttcg ctattatctg aattaggcgt acctcttaaa
     5821 gccattatgg agcgtgtagg acattctgat gcaaatacaa ctctgtctat ttacaatcac
     5881 gtaaccaaaa aagcaaaaca acaagtaatt gataaactaa ataggctttg atatttctgc
     5941 cccttttttg ccccttttac ttaaaataaa acaaaaaaag aaaagagaac cctgtaatat
     6001 aagggttctc ttttaattcc tacatcatac cgcccatcat tgatggatcc attgcaggag
     6061 ctgctggcgc tgctggttct ggtttatctg caacgactgc ttctgtcgtt aataacaagg
     6121 cagaaacgga agcggcattt tgcaaggcag aacgtgttac tttagttggg tctacgatac
     6181 ctgcttcaac catgtttacc cattctccag tagcagcatt gaaaccagtg cctagttcca
     6241 cgttcttcaa tttatcaaca atcactgatc cttcataacc agcgttttca gcgatttgac
     6301 gaattggttc ttctaaagca cgaacaacga tcttgatacc tgttgccacg tctccttctg
     6361 cttctacagc agaaactttg ctgataacgt tcactagagc tgtaccacca cctgagacca
     6421 ttccttcttc tactgcggca cgtgtagcat tcaatgcatc ttcaatgcgt aattttaatt
     6481 ctttcaattc tgtttcagta gctgcaccaa ctttaacaac agctacacca cctgctaatt
     6541 tagctaagcg ttcttgtaat ttttcgcggt caaaatcaga agttgtttca gcaatttggt
     6601 ttttgatcaa ttgcacacga gcttcgattg cttctttttc tccagaacct tctacgatcg
     6661 ttgtattgtc tttatctaca acaactttag aagcatttcc taagttttcg attgttgcat
     6721 ctttcaattc taaacctaga tcgtcagtaa ttactgtacc acctgtcaaa atagcgatat
     6781 cttcaagcat tgctttacga cgatcgccga aaccaggagc ttttacagct actacgttga
     6841 atgttccacg gattttgttc aatacaagag ttggcaatgc ttcaccatct acatcgtcag
     6901 caataatcaa caatggacgt gattgttgca agatttgttc taacaatggc agaatatctt
     6961 ggatgttaga gatttttttg tctgtgatca agatatatgg gttttctaga acagcttcca
     7021 ttttgtcgtt atctgttacc atatattgtg ataagtaacc acgatcaaac tgcatacctt
     7081 ctacaacatc tagttctgtt tcgatccctt ttgattcttc gattgtgatg acaccgtcgt
     7141 ttccaacttt ttccattgca tcagcaatca aatgtccaac tttatcagaa cccgaagaaa
     7201 cagctgctac ttgtgcaatg gcttcttttg aatcaacaac agttgagata ttatgcaatt
     7261 cttcgactgc tgcttttgtt gccaattcga tcccgcgacg aatgcctaga ggattagcac
     7321 ctgcagttac gttttttaaa ccttcgcgaa cgattgcttg tgtcagaaca gttgctgtcg
     7381 ttgtaccgtc tcctgcaata tcatttgttt ttgatgctac ttctgataca agttttgcac
     7441 ccatgttttc aaaatgatct tctaattcga tttcttttgc gatcgtcaca ccatcatttg
     7501 tgatcaatgg agagccgtaa gatttttcta gtacgacatt tcggccttta ggtcctaatg
     7561 ttactttcac tgtatctgct aatttatcta ccccgcgtaa catagctgcg cgtgcgtctt
     7621 ctgcaaattt caattctttt gccatgattg attcacctca ttgattattt ttgattattt
     7681 ttttatcttc caaatttact caacaatcgc catgatatct tttcctgcga cgatcagata
     7741 ttcttttcct tcgtatttta cttctgtacc ggcgtatttt tcgaacataa cttggtcgcc
     7801 tgcttttaca gcggcaggaa ctttttcacc gttttctaac aaacggcctt caccgactgc
     7861 aacaactgtc ccagtttggg gtttctcttt agcagcagaa gctaaaacga ttcctccgac
     7921 agttttttct tcttcttttg cgacttcgat aatgacgcga tcacctaatg gttttaacac
     7981 gataaatccc tccgataact ctttttttaa tctgttagca ctcttaacct tcgagtgcta
     8041 acctacattt ttaataatac tcattctccc tcttttttgc aagcttttaa atcaaagaaa
     8101 agaaatttta ttctgacagc tgcttattga aattatgata taattgaaat caatcgatat
     8161 aagcttgcct tgcatgcttg ttatccctta aaaggaggaa ttacatgtct ttaaaaaaat
     8221 atagcttcct cagcattttt tgctatggtc taatttttat ctcacccttg atcctagctt
     8281 cgataggact tgtaaaatct acgtctgaat tgatcacagc aactgcagtc atgtatatac
     8341 taggcgccgt tgtcctagct attttttatt ttaagcaacg tgaaccctta gcgatcgaat
     8401 cagcggtaaa aaaagcttct gctccaaaga ttgttattta tggattggtt ggaatctttg
     8461 tcgcattgat tctgcaaagc atcgctgtca tgttagaatc tgtattgttt ggcaaagcaa
     8521 ccccttccga gaatacacaa aatatcatcc agatgatcat ggaagcaccc gcttttattt
     8581 tggcgacaac tgttgctgga cccatcatgg aagagttcgt attccgccgc agtatactgg
     8641 ggatcatcag tcgatattca aatttttggg tcggagcagt gatcagctct ttgctgtttg
     8701 cgtttgctca taatgatggt catctgctaa tctacttttt cctaggtttc ttcttcagcc
     8761 tagagtacaa agcaactggt cggatctgga catcgatgat cacacacgta ggaatgaaca
     8821 cattagttgt tcttgttcaa ttagctgtcc aaaaagggct agtataaaca tgactataaa
     8881 tactcctgtc atcctagatt ggtgattttc ccaatctaaa gcgacaggag tatttttgtt
     8941 ttttagtcgt tcatcttttc tgctgctcca tcattttttg tctgtctttc ctttttgcag
     9001 tttgcagaat ctattttttt caaaaatggg aaataaatga aaaaactaat tgacaacacg
     9061 ataccttgta aaatcgctgc aggcactccg cctactaaga aacccgagag gatcggcggt
     9121 gttgtccaag ggacttggac tgctgtaaat aaaggaacga atccaaaata taaagcggaa
     9181 taggtaatca atcctgaaac gaccggtgcg aaaataaaag gaacagccat catcgggttc
     9241 atcacgatcg gtgtggaaaa aataattgat tcattgatgt tgaaaaaggc tggtaaaaga
     9301 gcaagatttc ctaattctct gtatttcttc gatttcgcaa aaaccgtcat aaaaaataca
     9361 gcccctaacg tcaatccagc acccgtaacc gtcatgaact gatcgagaaa ttgctgagta
     9421 acgatatgtc cgccatttgc caccgtcaac tcttgtcctg aggctaagat ttctgcattt
     9481 gctaatgaat tcgcttgcaa gacgggacta accaacccat tgaccatcgc agatccatga
     9541 agaccaaata accataaaaa cggaacaaaa aaacctaata aaagcacacc aaacaacgtg
     9601 tctatgaacg attgaatggg aagctggaca tatgtccata ctccttgaac aacagtcatt
     9661 ctgaacaaat gttgaaaaac cgctgaaccg ataagcgtaa aagtgactat aacaatgatt
     9721 ggaatcaatg aagtaaaaga ctgccaaaca ttttcaggta tcttctctct ttgcttccac
     9781 cctttcgcaa gtcttactaa acgtgcatag ccccatccga tgatgagccc agttgtaact
     9841 gctcctagta tcccttcagc acctgtccag acagtatcta tctgagtatt tagtggctga
     9901 ataagcagta gataagcaga caaggctgtt atgccgactg gtaagcgatc gatgtcttct
     9961 tcttttgcat aagcgtatgc aatggcaaaa acgatccaaa aggctatcac atcaaatgta
    10021 gcctggtata cttgtgtaaa atacatagaa aatcctgttt tgtctatcca ctgagcgatg
    10081 taatccacag gaaaatgagc aaataagaaa aacagcgagc caacaataat tattgggatc
    10141 gtgtaagtca ttccattacg caaagcagtt aagggtctaa gattcacaac agatgttatc
    10201 attggtaaaa aggcacgatc taaccactct ttcatttctt atctccttca acttctattt
    10261 ttatcaatca gttttcttaa tttaactata cccaatgatc acgaaccatg aaaggaatgc
    10321 gcactgagct tttcaaaaat cggattcttt gacgaatatt cagcaactgc ttacaatgga
    10381 caggaataaa aaaggaggtt ttttatgaaa attgcactca ttggagctac tggccatgca
    10441 ggttcattta ttttagacga agcattgtcc agagatttag acgtgacggc aatcgtgcgt
    10501 catccagaaa aattgccatc agatgttccg tttatacaaa aggatttatt tgagttgaca
    10561 actactgatc ttgcatcttt tgatgttgtc gttgatgctt tcaacgctcc caaaggaaaa
    10621 gaagaaatgc atcaaacttc tttgtcccat ttgattgaga tgcttgaagg aaccgataca
    10681 cgcttaattg tcgttggggg tgcctcttcg ttattcttag atacagaaaa gacgactcgt
    10741 atgattgacc aagtacctaa aaatgcaccc ttctatccaa cagctttcaa tatgtacgaa
    10801 ggattgttaa aattgaaaca atctaaaaat ctacattgga cttatatcag tccagcttct
    10861 ttatttgatc cacatggcaa acgtacaggt acttatttgt taagtgatga ttatttacaa
    10921 agaaacgctg ctagcgacag tacgatcagc atggcagatt acgcgattgc attagtagat
    10981 gagatactgg atgagaaaca tgaaaagcag cacatcagtg ta
//
LOCUS       DANKHL010000095        10930 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000095 DANKHL010000000
VERSION     DANKHL010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.098"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            62..1321
                     /gene="lysA"
                     /locus_tag="PW101_001714"
     CDS             62..1321
                     /gene="lysA"
                     /locus_tag="PW101_001714"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDL2514113.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1308..2108
                     /gene="dapF"
                     /locus_tag="PW101_001715"
     CDS             1308..2108
                     /gene="dapF"
                     /locus_tag="PW101_001715"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDL2514114.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2111..2992
                     /locus_tag="PW101_001716"
     CDS             2111..2992
                     /locus_tag="PW101_001716"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDL2514115.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            2989..3705
                     /locus_tag="PW101_001717"
     CDS             2989..3705
                     /locus_tag="PW101_001717"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDL2514116.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3711..4406
                     /gene="dapD"
                     /locus_tag="PW101_001718"
     CDS             3711..4406
                     /gene="dapD"
                     /locus_tag="PW101_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDL2514117.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4422..5423
                     /locus_tag="PW101_001719"
     CDS             4422..5423
                     /locus_tag="PW101_001719"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDL2514118.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5420..6781
                     /locus_tag="PW101_001720"
     CDS             5420..6781
                     /locus_tag="PW101_001720"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDL2514119.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6741..7892
                     /locus_tag="PW101_001721"
     CDS             6741..7892
                     /locus_tag="PW101_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDL2514120.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7867..9036
                     /locus_tag="PW101_001722"
     CDS             7867..9036
                     /locus_tag="PW101_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2514121.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9211..10590
                     /locus_tag="PW101_001723"
     CDS             9211..10590
                     /locus_tag="PW101_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2514122.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         3629 a         1911 c         2455 g         2935 t
ORIGIN      
        1 aaatctttat caaatgaaat tccaataaaa acggaaaaaa agtgaaaaga ggaatgaaaa
       61 aatgacagaa ttgataattg gcggagtcac agcaagcaca ttggcagaaa agtataagac
      121 acctctgtat gtgtatgatg aagaaaagat gagacaaacg atgctgtcat tcaagcgagg
      181 ctttgtatcg aaacgttttg atacaaaagt gctttacgcc tctaaagcat tccaaacgat
      241 tgagatgctg catttagttc gtgaatatgg attcgggcta gatgttgtca gtggtgggga
      301 aatctatacc gctttgcaag ctgattttcc tagagatact atctacttcc atggtaacaa
      361 caagacgcca gaggaactga tctatgcact tgaaaatgat ttgttgcata ttgttgccga
      421 taatttgatg gaagttgaat tattagcaga attaagccaa cgctatttaa aaaaaatctg
      481 tatcatgctt cggttgaatg tcggagtaga agcacatact catgaatata tcgtcactgc
      541 gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa gagtgccaac aagcccttca
      601 gttgataaaa gaaagcagct atctagagtt agaaggattt catgctcata tcgggtcaca
      661 aatttttgat gttactgctt ggttagctga aatcgacaaa ttgacgaatt acttaaccga
      721 ttttgaccaa cctctttcct tgaatcttgg cggagggttt gggattcgtt atacacaaaa
      781 tgatcagccg atcccagtcg aagaggcact aaaaaaactg attgaatata cggaagaagc
      841 tttggaaaaa cgaaaactga ccattcgtca attactgatc gaaccgggta gaagtattgt
      901 tggagaagct ggtacaacac tatacacgat tggtttcatc aaaaagacac cacataaaaa
      961 atattacttt gtagatggcg ggatgacaga taatatccgt ccagctctgt atcaggcaga
     1021 atacgattgt gatttagcag caaaactgtc ggaagaaaaa acagagaaag tgacgattgc
     1081 aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa gcttatttgc cgaaagcaga
     1141 atcgggtgat cttttagctg tttattccac gggagcttat ggatactcga tgagcagcaa
     1201 ctacaatcga gcgacacgac cagcagttgt ttttgtcaat aatggccaat caagactgat
     1261 tgttcgcaga caagcatttt ctgatctact aaggggggaa gtttcctatg atccttcata
     1321 aatataatgg ctgcggaaat gattttatct tattagatta cgcagaagac acagattata
     1381 gtcaattagc cgcttcttta tgcacaaaag aaaaatatga tatagacgga ttgatcgccg
     1441 taaaaatcga tccgttggaa atgatctatt acaacaaaga tggcagccgc gcgccgatgt
     1501 gcggaaatgg cattcgctgt tttgctcgat acgtaaatga tcagaacatg atgaatgaac
     1561 aggaattcaa cgtagagaca ttagcaggca caagaaaaat tacggtgttg gatccctcac
     1621 ctttttattg cagcgtagat atgggagtgc cagatgattc tccggaaaaa atcaaagcta
     1681 atcaaactta tccaataaaa aaccaggaaa tagagatcaa tggtgaaaaa gtaacgatca
     1741 ctagtttatt tttaggaacg attcataccg ttgtttttgt tgaaaatgcg cgtgaggaag
     1801 tctggagaaa aagaggtgaa ctgatctgtc atcatccttt gttcaaagaa aaaaccaatg
     1861 tcaactttgt agaagtagtc aatgaacaag aattgatcgt ccgtacctat gaaagaggag
     1921 taggttggac acttgcttgc ggtacaggtt gctgtgcctc ttatgtggtt gctaaggaaa
     1981 ctgggaaaat ttcagcagat caggtagttg ttcacttgga acaaggagac ttgatgatct
     2041 ctggtgagga tgcgatacaa atggctggcc cagcagttca tgaatggaca agaatattgg
     2101 aggaatgaag atgataaaag gttcgatcgt ggcgctgatc acgccgatga atgaagaggg
     2161 atcagtagat tatgctggtt tggaaaaatt gatccaattc catttagatg aacaaacaga
     2221 tggattatta gttttaggta caacaggaga atcttctacg ctcacacaat ctgaggagga
     2281 gcagatcctt caactgacag taaagaaagt ggctggccgt gtgccggtga ttgcaggagc
     2341 tggaacgaat aatacgaaag aaacgattga aaaagcgaag cattttgctt ctttgggggc
     2401 agacgcactt ttggtcatca ccccttacta caacaaaaca agtgatgcag gactagctgc
     2461 tcattttaca gcaattgcag aagcctcacc gattccgctt attttgtata atgttccttc
     2521 tagaacaggc atgtcgattc caatccatgt attagtaaat ttagcagaac atcctaatat
     2581 cattggtttg aaagaagcct caggagatat ggcttatgtg atggatgccg ctcggttgat
     2641 tggagaagaa ttcttccttt acagtggaaa tgatgatttg atcctaccag taatgagtgt
     2701 aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa cctaaaatag tccatgaatt
     2761 agtaaaagat acgcaggacg gaagatggca gcaagcaaaa gaaaagcaat taaatgcact
     2821 tgaactgatc catgccttgt tcagtgagac caatcctatt cctgtcaaag cagccatgtc
     2881 attattagac ttaccttccg gacctctacg cctgccttta gtttctcttt caaaagagaa
     2941 aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg gttgaaaaat gaaaatcgga
     3001 ctgattggca gtggacagat gggaaataga gtcgaagaag ttgcacaaga aagaggagat
     3061 acggtccttc ttttacggac ggcacctaaa gaagcaacta cgcttgcttt tacggaactg
     3121 ccagaactgc ttattgattt ttctcatcca gataatttag agaaaatcct ttcgtatagc
     3181 ttatcatatc aacttccgtt agtcattggc acgacaggtt atacacaatc acagttccaa
     3241 gaaataaaag aacatgccaa acatgtacct gtgatgtata gtgctaattt ttcgttcggc
     3301 atcatggtga tgaatcaact aatcaagcaa gcagctgcaa tgttgcaagg ctggcagatc
     3361 gaactgattg aaaaacatca tagtcgtaaa aaagatgcac catctggtac tgcaaatatg
     3421 ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg tatacaactg gcaagggaaa
     3481 aaaagagaag aaaaccaaat tggtgtacac agtattcgtg caggttcttt acctggtgaa
     3541 catgatgtat tgtttgctgc tactgatgaa atatttacga tcaaacatga atctttttct
     3601 aatcgaatct ttgctgcagg cgcagtagaa gcagcggact ggttgaaaga tcagtcacca
     3661 ggttattaca aattagaaga tttattaatg gacaaagggg tgtaaaaaac gtggaagctc
     3721 aagaaatcat ccgctacatt caaacggcat ctaaaaagac gcctgtgaaa gtctatctga
     3781 atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt tggtgaagga agcagcttcg
     3841 ttgtttttgg tgactatgag acgattgctc cggttttaga agctgaatca gaaaaaatca
     3901 ttgaaatcga aatcgaaact gctgcaagat acagcgcagt tcctttgcta gatattaaaa
     3961 aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga tcaagtgtca atcggcaaca
     4021 atgccgtgat catgatggga gcgattatca atattggtgc agtaatcggt gaaaacacga
     4081 tgattgatat gggtgctgtc ttaggtggac gagcgacagt gggcaaaaac tgccatattg
     4141 gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc ggccaaaccg gttatcgtag
     4201 aagacggcgt actggtaggt gcgaatgcag tgattgttga aggtgtccat atcggaaaag
     4261 atgcagtcgt ggcagcagga gcagtggttt tagaagatgt ggcagcagaa actgttgtcg
     4321 gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca gacaaaagaa aatacagcat
     4381 tgattgctgc gttacgcgaa ttatagagga gatggagaaa aatgcgcgaa atagatacct
     4441 gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct caaagtgtta gaagaatatg
     4501 attttccagt aggacgattg aaattattag cttcaaaacg ttcagccggt aaagtttgtg
     4561 tattcaagaa aaagaaacac actgtacagg aattgacaga aacttcattt gaaggatatg
     4621 atttagcgct attttctgca ggtgcttctg tatccaaaga gtttgcgcca atcgcacgaa
     4681 gtcagggact atttgtcata gataattcct cagcatggcg ggaagattcg gatattccat
     4741 taatcgttcc agaggtcaat ctggaagact atacattgaa tcggctgatt gcaaatccaa
     4801 attgttcaac cattcaagcg gtacttccgc ttaaagcttt acaagaagcc tttggtatcc
     4861 agcgagtcaa ttattcgacc tatcaagcag tttctggttc cgggcaaaaa ggcattgaag
     4921 atcttcatcg tacgcaaaat gacgaggaac cagttttgta tccttataat attagccaaa
     4981 cggtgattcc ggaaatcgat cagcctttgg aaaatgggta tacaaaagaa gaacagaaaa
     5041 tgataaatga gacccgtaag attctgcatc agcctgcttt gccagtatca gcaacctgcg
     5101 tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc agttcaactg caaaaagagt
     5161 ttacgacgga agaaattaga gagtgtctca gccagtttcc agggattcaa ttagaagatg
     5221 atctagcagc acataaatat ccgacgagca ttcttgctag aggaacagat atggtttatg
     5281 ttgggaggat cagaaaagac acttctttag agaacggatt gttgttctat acgactgccg
     5341 ataacatacg caaaggagcc gctgcgaatg ccgtacagat tgctgcagct ttaatgcaga
     5401 aaatagcgga ggtggcaaga tgagtaatac gctgaaagta gcgaaattcg gcggaacatc
     5461 gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt gttctttcag atcccaatcg
     5521 ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca gatgatgaca aagtaacaga
     5581 cttactttat ttgatttatg cttaccttca gcatcatgtt gattggacac ccctttggaa
     5641 aaggatttgt gatcgtttca ttcaagtaag agatgttctt gccctgcagt tacctattga
     5701 agatgaacta gcacgcatac agaaacaact ggaagacgaa aagataactg aagattattt
     5761 agtaagtaga ggtgaatatc tgactgctca actgatggct gagtatcttg gttaccagtt
     5821 tattgacgca aaagacctga ttttctttga ctatgatggg aaaatcgatc aggaaaaaac
     5881 agcacaggaa atcacaaaag catttaaaag atatccgaaa atggttgttc cagggtttta
     5941 cggggcgaac cctgcaggaa aagaaaaatt attgggccgt gggggaagcg acattaccgg
     6001 cgcgatactg gcgaagatgt tgcaagcgga aagttatgag aattggacgg atgtttcagg
     6061 aatcatgatg gctgatccgc gaatcatcga tcatcctaaa atcatcgagg aaatctcttt
     6121 tagagaactg agagagatgg cttatatggg ggctaacgtt cttcatgaag cagctgtttt
     6181 cccagttcaa gaggctgata tccccatcca aataaaaaat accaatgatc ctttcgctca
     6241 agggacaaaa atatcgaatc acgaaataga aaaggaaaat ggactgacag gaatagctgg
     6301 acgcaaggat tttctttcca tcactttatt caaacgtcat atgtccgatg aaatcgggtt
     6361 tatctggaaa gcaatgagca tttttgctaa acatgagata tcaatcgaac atattccttc
     6421 agggatcgac aatatcggag tgatcgtctc agcagaagcg attgccgata agttatttct
     6481 catcacgaaa gaattaaaag aaacacttgg cgtggaagaa atcgtcgtga tcgaagactt
     6541 agctttgatc tctgtcgttg ggggtccgca taaagaattg attggattat ctgggaaagt
     6601 gttatcgata ttgaacaaat tagaaattcg cacatcgatc ttatcacaag gagcacagga
     6661 gttgaatctg atcatcggtg taccgaataa tcaatatgaa acagtagtga agggtatcta
     6721 tgaagggatg gtgaatacgg atgctgaaag aatatcgaat ggcactccat caaattcctg
     6781 aaatcgggtt tcatgaattc aagacaaaaa aatatttata tgaacaagta aaaaacagcg
     6841 aggccgttat ccatgaaatc ggcgaaacag gactactcct ttattttgat aatcagcaag
     6901 aagaaacgat tgcttttcga acagatatcg atgcactgcc agtcaaagaa gaaacgaatc
     6961 tctcttttgc ttctaagcat gaaggttata tgcatgcatg tggtcatgac ggacatatgg
     7021 cgatgctttt agggttgact gactttataa ctaaacacat tgaggatctt atttataatg
     7081 tagtactgat ctatcagcca tcagaagaaa tttccggtgg cgcacaaagc gttattcagt
     7141 caggattgtt ggataaatat gaagtacaag caatatttgg ctttcatcta tggcctggac
     7201 tagctaaggg agaagttttc tctcgaccag gacctttgat ggcacaaagc agtgaaacag
     7261 acattatcat taaaggccag tctgcacata ttgcttctag tgaaaaagga atcgatagtc
     7321 tggaggcagc tgtacgattt ttgaagaatg tttatgattt tgaccggcaa ttaccggcag
     7381 agtggaatca tctaatgaaa tttggccaaa tccaaggtgg aacgatccga aatgttttag
     7441 ctggagaagt tattgtatcc ggaagtatcc gttcttacag ttcagatatg caaaattatt
     7501 tgaagagggg attagaccat ttagcagaac agtttcactc tacagctact atttcttttc
     7561 gctataatga cggctatcca gctgtaatca accatagagg cttatatgca gcactgttag
     7621 attcaaacaa actacatgaa ttagatgctc cagttcttca agccgaagat tttggcatat
     7681 atacgcaaca atatccatgt gtatttttct ttttaggtgt cggtgatacc cctgctttac
     7741 atgatccgac gttcgatttt gatatggatg ttttagaaaa tggtgtagaa tggtacaaaa
     7801 cgattctttt tacagaaaat cttcttgtaa aaatcgatag cagttcaaaa gaggagcgag
     7861 taaataatga tgaatttatc caatcgattt agtcctaggc tttctagaat agaagtatca
     7921 aaaatacgac aattcgatca gcaaatatcg tcgataccag atgtgatcaa actcaccctg
     7981 ggagaaccgg attttccaac tcctgaacat gtcaagcaag caggaatagc agcgattgaa
     8041 gaagactttt ctcactatac cggaatgaga ggattagagg aactgcggga agcggcttgt
     8101 atatttcagc aacagcgtta tggattaact tatgatccgc aaacggaagt tttgacgaca
     8161 gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg ttttggaaga aggagacaag
     8221 gttcttattc cagcgcctgc ttattcagga tatcagccat tagtggaact agctggtgct
     8281 gaattgatcc cgatagatac ttctgacaca gggtttgtct gtcagccgga acaatttgaa
     8341 cgagcatttg aacaatatgg atcagctgtc aaagcagtca ttttaaacta tcctaacaat
     8401 cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa tcgctgaggt tttaaaaaaa
     8461 tatccggtat ttgttataag tgatgaagta tatgctgaat tgacttatag tggaacgcat
     8521 atgtccatag ctacttatct tccagaacaa acaatcgtga taagcgggtt atccaagtca
     8581 catgcgatga ctggttggcg agtgggcttt atttttgctc aaaaagccct tattgacgaa
     8641 ctaattaaag tccatcaata tctggtgact gccgctacaa cgatgtcgca aaaagctgcg
     8701 gctgaagcgc taataaatgg ggtgaaagat agcgagaata tgaaagagca atatcaaaca
     8761 cgaagagact atctggtcca gcaattggcg cctttaggat ttgaagtgac ccagccaaac
     8821 ggtgcatttt acttgttttg caaactccca gctgccattc aaactgactc ttggcagttt
     8881 tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc caggaattgc atttggccca
     8941 gaaggagaag gttatatccg gatttcttat gctagcagca tggaaaaatt acatgaggcc
     9001 tgcaaacgga tcaaagactt tttaacagaa cagtgattca tagattttat caaaaaaaca
     9061 atcgttaatt ttgcggtata ttctgcaagg agacgattgt tttttaaatt tagttcaaat
     9121 gaacgcgttt cttttgattc ataactaatg taatgttaag tttaaattgt ttagaatgat
     9181 tttaaaagaa tattcaagga ggtttcatca atggaaaaaa tcaaagttgt cattgtaggt
     9241 gcatctcatg gagggcatca atcgatcttg gaactattga cacgatacgg tgaaaatgta
     9301 gatatcacgc tatttgaagc aggagactat atttccttca tgtcatgtgg tatggagctg
     9361 tatttagaag acaaggtaac agatgtaaat gatgtacgta atttcagacc agaaaacttt
     9421 ccgcaatcca atgttcatat tttaaataat cacgaagtaa aaacgatcga tgccgataaa
     9481 aaaacagtaa cggttactag cgcatcagat aacgaaacaa aagaatatcc ttatgacaaa
     9541 ttgattttga gttctggtgt caaaccaaac aatctgccag taccaggagc agatctagaa
     9601 aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa tcaaagaaaa attaaatgat
     9661 ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca ttgggatcga agcagcagaa
     9721 gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg tgatcgatag acctttaggc
     9781 acttatttag atgctgagat gacagatatt ttagaacaac acctaaatga aaacggtatt
     9841 gaagttttga caaatgcaaa aatcgaagct tttactggta acggaaaagt tgaggcaatc
     9901 aaaacaagtg aaaaagaaat cccagcagat ctagtcattc aagctgccgg tgtgaaggca
     9961 aatacagaat ggttaaaagg gatcgtcgac ttagatgaac gtggctggat ccaaacaaat
    10021 gaatacttgc aaacgaatct tccagatgtg tatgcagtag gagatgctac acttgcttat
    10081 tctatcccag cacgcaaaaa aatgccgatc gctttagcga ctgttgcacg tagagaagca
    10141 cgttatgtag taaaacatct ttttgaagaa gtaccaaaca aaccgttcgg cggagtcgta
    10201 ggttcctctg cattaagtgt ctttgactat cattttgcag caagcggatt gaatagcttt
    10261 acagcagcaa aagcaggagt agatgtacga gtgtcttatt atgaagatac gttacgacca
    10321 aaatatgtac cagaagaaca aggaaatcca aaagtgtcca tccaattatt ctcggatcca
    10381 atgacccatc aattgctagg aggagcagta ttatctacct atgatgttac aggtcaagga
    10441 aatgtattgg cactagcgat ccaaaacaaa ctgactttag aagaactagc agaagctgac
    10501 ttcttcttcc agccaggctt tgaccgacaa tggagcatgt tgaatctagc cgctcaacaa
    10561 gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa tatttgaaaa gaggttgtga
    10621 aaaagctgcc ttgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt
    10681 aacttggctg gacttattac cgcagatgca agcttttgaa caccgtttat tttcaaaaaa
    10741 gggactgtga cacgatttgt gtaccgaccc ccaaaagtta gaccaaaaaa tctaactttt
    10801 ggaggtcggt attttttatg gttaaatata cttttgaact taaattaaag atcgttcatg
    10861 attatttgga tggcaaaggc gattggaccg taaaaatcca gatcaagaga ttgaagcgga
    10921 aatgcttaaa
//
LOCUS       DANKHL010000096        10831 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000096 DANKHL010000000
VERSION     DANKHL010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.099"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(66..290)
                     /locus_tag="PW101_001724"
     CDS             complement(66..290)
                     /locus_tag="PW101_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HDL2514123.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(488..2068)
                     /locus_tag="PW101_001725"
     CDS             complement(488..2068)
                     /locus_tag="PW101_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDL2514124.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2469..3845)
                     /locus_tag="PW101_001726"
     CDS             complement(2469..3845)
                     /locus_tag="PW101_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL2514125.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3973..5091)
                     /locus_tag="PW101_001727"
     CDS             complement(3973..5091)
                     /locus_tag="PW101_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2514126.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5233..5667
                     /locus_tag="PW101_001728"
     CDS             5233..5667
                     /locus_tag="PW101_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2514127.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5745..6278)
                     /locus_tag="PW101_001729"
     CDS             complement(5745..6278)
                     /locus_tag="PW101_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDL2514128.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6372..7550)
                     /gene="mutY"
                     /locus_tag="PW101_001730"
     CDS             complement(6372..7550)
                     /gene="mutY"
                     /locus_tag="PW101_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDL2514129.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7543..8340)
                     /gene="recX"
                     /locus_tag="PW101_001731"
     CDS             complement(7543..8340)
                     /gene="recX"
                     /locus_tag="PW101_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDL2514130.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8447..9829
                     /gene="rlmD"
                     /locus_tag="PW101_001732"
     CDS             8447..9829
                     /gene="rlmD"
                     /locus_tag="PW101_001732"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL2514131.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10003..10581)
                     /locus_tag="PW101_001733"
     CDS             complement(10003..10581)
                     /locus_tag="PW101_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HDL2514132.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
BASE COUNT         3189 a         2198 c         1846 g         3598 t
ORIGIN      
        1 aaatttttgt gaattccagc ttatttcttg gagttaaacg cttctgtctc agcctctttt
       61 tctttctagc tttttgattt ttctcttttc ttttgtactt ttctcttttc attttcagtt
      121 ctacgcgtgt ctttattttt aactttttcc tctttatgga tgattgatcg tactttttgt
      181 gcaatttgct cgtttttgtc ccagtctttt acatctaata atcgacataa tcgtaacatc
      241 tcttcttttt tctgtttttt tctttcacgt ctttgccatt ctgtttccat gtgtcacact
      301 ctcctttctt taatcatatg tacgcaaaat aatttttata tttttttgat tagacatttc
      361 tatgatttta atatagtaaa aagagccgtc acggaaaatc cgtcacggct ctaaacaaaa
      421 tatgagaggc tgtttttgtt tggctattct tattacttga tgcagggcag cttgtgtcaa
      481 gacttcttta catcaaactt tttaatttta cattgggata tttatccgca aaccagcgtt
      541 ctgcaaattg gttctcgaat aagaacaacg gttggtcgaa acggtcacga gccaagatat
      601 tgcgacttga actcatcttc tcatccaaat cttctggatc gatccagcga gcgattttgt
      661 gtcccattgg agacatgatt acttcggcgt tatactcatt taacatacga tattggaata
      721 cttcgaattg tagctgacca acagctccaa tgatatattc ttccgttaga tatgttttat
      781 aaagctgaat cgctccttct tgaactaatt gatagattcc tttgtggaag gatttttgtt
      841 tcatgacatt ttttgcagtc actttcataa acagctctgg agtaaatgaa gggagttctt
      901 cataagcaac ttttagtttt ccttcgaaca atgtatcccc aatctgataa ttgcctgtat
      961 catatacacc gatgatatcg cctgctactg ctttttcgac gttttctctc gaatctgcca
     1021 taaattgtgt gacattgctt aatttcagtt ttttatttgt acgttccaac cagacatcca
     1081 ttcctcgttc gaatgttcca gaacagattc ggacaaaagc gattcggtca cgatgggctg
     1141 gattcatatt tgcttggatc ttgaatacga aacctgaaaa ttcttcctca taaggactta
     1201 ccatctgatc atcttgtgtt ttgtgtccat aaggagctgg tgccaattca acgaaagtct
     1261 ctaaaaaagt ctgtacaccg aagttagtca atgcagaacc aaaaaatact ggtgtctgat
     1321 cgccacgagc aattttttct tgattaaatg catctccagc ctctttgatt agttctactt
     1381 cttctaagac ttgttcatat aaggaatttt cagttagagg atgattttcg ggaagcagtc
     1441 catcttcatc caattctact aatcgttcat cctgataatt ttccggacga taaaactcta
     1501 cacgattatg gtagatatca taaagacctt ctagcccttt acccataccg attggccaat
     1561 tcatcggata agattcgata tctaatagct cttctaattc ttctaacaat tccaatggtt
     1621 cacgaccatc acgatctagt ttgttgataa aagtaaatat cgggatgcca cgttttttta
     1681 caacttggaa taatttcttt gtctgtgctt cgatcccttt tgcactatcg attaccatga
     1741 ccgcactatc cacggccatc aaagtacgat aagtatcttc agaaaaatct tcatgtcctg
     1801 gtgtatccaa gatatttact cgtttccctt gataatcaaa ctgcataaca gaactagtaa
     1861 cagaaatccc acgttgtttc tcgatttcca tccagtctga tttggcaaaa ttacctgttt
     1921 ttttgccttt tacggtcccg gcttgacgga tagccccacc aaacaatagt aattgttctg
     1981 taatcgttgt tttcccagca tccggatggg aaatgatcgc aaaagttctg cgattgtcca
     2041 cttgttgctt taattttgga ttattcataa cctaactcca tttcttccat tcaattattt
     2101 atattttctc taggcacaga ttgtccgatc tcttaaagaa agacaatttc atatagggct
     2161 gtgacaaaag ccgtgtagca gtctcttttt ccgagtaaac ggcgttctag agacagccta
     2221 tcagcaataa gcccacccaa gccaaaaata cgaaaagctg tttttgcttg gctgctctta
     2281 ttacttaatg caggatagct ttttcacaac ctttgtcaac tactacaact gaatcagtat
     2341 agcatggatt cagcgaatga aaaagccacc aaaccataag agtcaaatat ttttttgtca
     2401 gctgactaaa agaaaagacg tgttgcttgt ttattcaagc aacacgtctt tcataaccaa
     2461 gtttccaatt atcttcttgg ctcgtcatct tcgaattctt ttctgaagta tcttcgttct
     2521 tcttcaggtt ccgcttctgt ttcttccgga tcatgaaaaa tgacacggat tttaaggatt
     2581 cgagaacctt ccatttcttc agaaaccagc gtaatattat cgacatcaaa cgataacttt
     2641 tcgccttcat ccggaatcat gcctaatgct gtaatcaaat aaccagccat cgtatctaca
     2701 tcactcatat gcagatcact gtcaaaagct tcattgaact catcaatcag catacgtcct
     2761 tggatgatat attcgtgttc atcgattttt tcataaagat tctcaacttc atctgattca
     2821 tcatcgattt ctccgacgat ttcttctaaa aggtcttcta gtgttgctaa cccaacgacg
     2881 ccgccatatt catctaataa aatagccatc tgattctgcg tctttttcat ttcatataac
     2941 agatcatcaa taaagaccgt ttcaggaacg aataaaggct cctgcatgat tttcttgata
     3001 tctaaattat caaagccgaa tttatgggca gcttttagta aattctttgt atgaaggatt
     3061 ccgactactt tatctttgtc ttcgttataa acagggattc ttgaatagtt ttcggataaa
     3121 acttcattca cattttcttc cacagtgtcg ttgatatcaa tcataaatgc atccgttcgt
     3181 gggaccatga cttctcgggc aactttcgta tcaagagaaa aaaccccttg gagcatttct
     3241 aattcttcat tattcaatac gccttcgttt tcaagcatat aacgcatttc atctctggtc
     3301 atttttgaat cttcatcatc aaaagtcatc ggcgtgatac gcgccaataa acttgtggaa
     3361 gcagaaagca gccagacgaa gggcttagct actacaccaa tcaagcgtat cagccctgaa
     3421 gtgaactttg cgacttcttc tgatttattt aaagcaatac gtttagggta caattctcca
     3481 aaaacaatcg atacataggt caaaatagcc agcacaatga tatttgcaat attttttgcc
     3541 gctgtgccgc cacctaatac cggagctaac cgttccgata aagtattagc tagtgaagca
     3601 ccagacaaaa tgttgaccaa agtgattcct acttggattg tcgataggaa attatttgga
     3661 ttctgcaata tttttaaaag cttttgagat ttcacatctc cttcatcagc tttttgctcg
     3721 attcgattct tattgatcga aacaacagca atttcggaag ctgctaaaaa agcattgatc
     3781 aatgtcaaaa cgatcaataa taaaatctgc gctaacagcg actgactctc agggtcagca
     3841 ttcataatcg cattctctcc ttataaaatt aatttttagt ttacgataga aaaaaacgtg
     3901 gcactatagc cacgttaaaa tggtatcaca aagcaatttt ctttgcaata aaaacaagtg
     3961 tactaaaact acctacgagg tcgtagcaat tttcttcttg ttttcatttt gcttgtcttc
     4021 tttgacgatt ttgattatat aggaaatgat cgtcttgcag attgcgtaaa aaggaacacc
     4081 taagaagata ccaagcaatc cggcaatatt tccagctact agaagaacga taatgatcgt
     4141 taatggatgg attttcagtg atttcccaat tacattagga taaatgatat tcccatccaa
     4201 ctgttggacg attagcacaa ccacacaaca aagtaatgct tttaccggat cattgaagac
     4261 tgttactaga aatgcaggtg ctagtcctag ataaggtcct aaatatggaa ttaggtttgt
     4321 caatcccgca atgaccccaa ataagaaagc ataatccaca ccaattacca tatatcctaa
     4381 aaatgtgaag gtaccaacga ataaacattc gattgcttgt ccgctaatgt aattagccaa
     4441 tgttttattc agttgcccca ataactccac aatttggttt cttctttttt ctgggaaaaa
     4501 ctgttggatg tttggcacta gtttttctcc gtctttcaac atatagaaca agatgaatgg
     4561 tgctgtaatg attactacgg ttgccgaagc gatcgttcca acgatcgaac ctaaactatt
     4621 ggatactcca ctaaggaatt gctgaatgat tgtcccgtaa gaaatgtcta attgctcaaa
     4681 atatttattg atatctactt ccttgaacaa aggcaattcg gctacctctt ttaaccagct
     4741 ttctacctgt ttgatgaaat caggcatatt agatgccaat gaggaaattt gctgtactag
     4801 acttgggatg acactaagta aaatccaaat aattgctgca actaaaagga gcagcacaat
     4861 tgcgacagcc catattcttt tgatttttgc tttcatcaac agcttgacaa taggattcaa
     4921 taaatagtaa agaaagcctg caatcagtac aggtgcaaat aacgtggaaa aaaatgtccc
     4981 gatcggttcg aaaataaagt ttatttctga tgaaatcatg atcaatgcag ctatgatcaa
     5041 taattcaagc gaccaaaaca ttagtttaga ctgtctaagt ttttcaaaca atagatttcc
     5101 tcctttcttt cttactagta tatattatat tcgttaaaaa ttcttttatt ctttttttat
     5161 ttttgaaaac ttgccatact ttttatttgt tttattttcc agtatactat acgaaaggaa
     5221 ggtggctaga ccatgaaagt tgtacaaaca aaagatacaa tgagcaatat ttacttagat
     5281 gctgtacgga tcaggcatca agtttttgta gtcgaacagg gtgtgccttt gtctcgagaa
     5341 atagacaaag atgaagccca ttgtatccac ttcgtcctgt actcagataa aaaagaaccg
     5401 caaggaactg tacgtctttt gcctttagaa aacggaaaaa tgaaattaca gcggatggcg
     5461 attctttctg aatacagaca ccaagggtta ggaaaaatat tgattgaaga agcagaaaac
     5521 tttgctaaga atcaaggcta taatacgatt ttattagggg cacagtccac agctgaaact
     5581 ttttacgaaa aactaggata tacagcttac ggcgatcctt ttgaagatgc tggcatgcct
     5641 catatttata tgaaaaagac tttataaaaa tttatttcga aaaaagcaaa aaacagcaag
     5701 cttttgagaa aaatctctca ctggcttact gttctttttt gtgtttactt acgcgacaat
     5761 tgcttgtaac ggttatacca aatatcaata tagccttccg agaacgggcc atctccatta
     5821 ttgatccagt caaccagaat cttcacattt tcttttagga tgaagtcaat atcgttcggg
     5881 taattcatca ttttgctgtg catctcatat tcatcgacat ccaatagtcg tttctcgccg
     5941 tcaggaaata ccttgatatc caagtcatag tcgatatatt tcaaggcctc atcgtccagc
     6001 aaaaaaggtg aagctagatt acaataatag gaaaccccct tttctctgat cattgctatt
     6061 acattgaacc agtatttttt atgaaaataa acaatcgctg gttcgcgcgt tacccatcgg
     6121 cgtccatcag attcagtgac taatgtgtga tcattcacgc cgatcataga gtgttcgctt
     6181 gtttttaata ccatggtatc tcgccacgtt cgatgcaatt tgccgtcgtg cttgtaactt
     6241 tggatcgtca caaactctcc ttcttttgga actcccatag caacccttcc ttaagcagca
     6301 ttttgtgctt tgtgtttggg tgttcattac accgacatca taattattat atcaataaaa
     6361 atgagctttg cctagaaagg attcctattg tttcgatttt tctctaactg atctaccagt
     6421 ttcatttgga ccttaggaaa aacgaccgaa tcaaatgcat caggcttcaa ccacttcgaa
     6481 gttttgttct cggtaaattc ttgttctgct ccctcggttg cccgtccgta aaaaaggaga
     6541 acatgccatt tcagatgact aaaaatatga gtaacttctc ctaaatgtcg tgtctgccaa
     6601 acaaagagtt cctgcttttc aaatgggaga ttttgctctt cttctgctac taggtcatca
     6661 aacagatcga tttctggctt tgtttcccat tcttttttca atcgctcata ctcctcttgt
     6721 gtcacttcca tcattggaaa tgtccacata ttcgccagca atttttggct atctctttct
     6781 tcaaaataat acgctccaga atgattttga agagctgcac tgatataata aacgtctttt
     6841 ggctttgctt tttttgtttt tacaggaaaa ctcgtttgga ttcctcgttt gttagctaaa
     6901 caaaaggctt ggatcggaca agtctcacac tttggagaag ttggtgtaca gatagctgaa
     6961 cctagatcca tcattgcttg gttgaattca cctggatgct tttcatcaat gattttccgc
     7021 atcgcttcat caaagatttt cctgctagaa gcttttgcga tgtctgcttc aatgcaaaac
     7081 aatctgctga ctactcgcat cacgttgccg tcaactgcag gttctggaag tccaaatgcg
     7141 atacttgcaa tcgcgcctgt cgtatatggt cctattccct tcaatgagct aatttcttca
     7201 ggcgtttgcg gcatttcccc atcaaattct gacatgatct gtttagcagc agcttggatg
     7261 ttccgggctc ttgagtaata accaaggccc tcccatgctt ttaaaagctt ttcttctgga
     7321 gcatttgcta gctcttcaat tgtaggaaac cattccatga aacgataaaa ataatcgatc
     7381 actgtgtcta ctctggtttg ctgaagcatg atctcagaga tccagatacg ataaggatca
     7441 cgattatagc gccaaggcag gtttcttttt tcttgttcat accattgaat gaactgatct
     7501 tgaaattctt ttgtttcttt atctgtccat ttttcccatt ctttattcac tttcctcttc
     7561 cttttccgta atgaatcgct ggatcatatc caaatcaaat cctttttgat ataagctttg
     7621 ctttactttt agatttcttt ttttaggttc aaaccttaaa tttcttcgcc atagtttatc
     7681 tccttgtttg actaaataat catattctat ttcttgatct gcttcttgcg gaagatcagc
     7741 tatcgcttgt tggatcactt cttgagaaaa gcctttcgtc atcaatgttt gttggatttt
     7801 tctcaataat tctttttggc tttttccgtg attttttcca aacgcttttt cggctgtctg
     7861 atgtgcattt tgaacttggt cttcaaatgt atattggctg agcgcttgtt caatgatttc
     7921 aggtttcaaa cctttttgct gcatctgctg cgctaatttc tttggtcctt tgtcacttaa
     7981 gcgcatattc gtgcgcacat aactttcccc atagaccaaa tcatcgacaa ggtccaattc
     8041 ttttaagcgg gctacgattt tatttcgatc ttcttgaagg atttcctttt cttttaagta
     8101 tgttcggatt tctttttccg atcgcaactg ataacttaag taattcattg ctaggtgaaa
     8161 accgaaatca gctccgctac tcttcttgat ctcttggata gtttcttcgt ctaattcccg
     8221 tccttttaat aatcgatagc gtactaatac atcttcagat acttgtaaag cttctccttc
     8281 agataaatct acgcgataga aagggccttt gcctttactg attctcgtaa cggttaacat
     8341 aaacatcctc ctcgaacata tattctctat cataccacag aaaatccccg taatcaggta
     8401 ttatatgtta gaataaacca cagaaaaaag aaaaagggag atacttatgg cagaaacaat
     8461 tgtcaaaact ggacaaacgg ttcctttgaa aatcaagcgt ttaggcataa atggtgaagg
     8521 aatcggttat ttcaaacggt tgatcatatt tgttccatat gcactaccaa aagaagaagt
     8581 actagtgaaa atcacgaaag caactcctcg ctatgcagaa ggagaattaa taaaagtcaa
     8641 aaaaacaagc aaagatcgcg tagtggcacc atgtcctgtt tactatgaat gcggtggctg
     8701 tcaattacaa cacttagctt atcaagctca attggatttt aaaaaggatc tgcttttgca
     8761 agcattggaa aaattcaaac cagcaggctt tcgatcttat caacttttgc cgacgatcgg
     8821 gatggatgag ccttggcatt atagaaacaa agcacaattt cagttacgaa aaaataagca
     8881 gactcaaaaa attgaagctg gtttatacga agcaaactct catcagttag tccctataac
     8941 agactgcttg gttcaagaac ctcgaacaca aaaagtaatg aatactgttg ttcaactatt
     9001 gaataaattt gatgcaccca tctatgatga acggacaaac agtggaatct tccgcacaat
     9061 catggttaga atcggtgtta aaactgggga gttacaagtt gtttttatta ctcgcagtaa
     9121 aaaattcccg caaaaaaacg caatgatccg tgagatcaat gaacgactgc ccgaagttgt
     9181 ttcaatcatg caaaatgtcc aggcacaaaa aacgtctctc gtgatggggg atgaaacgat
     9241 ccacttatgg ggaaaagaag ccattgaaga acaaatcaat gaagtgactt tcgatctttc
     9301 tcctcgtgcc ttctttcaac tcaatcctca gcagaccgaa ttgctttatg gcgaaggaat
     9361 caaagcatta gatgtccagc ctaatgaaac gatcgtggat gcttattgcg gagttgggac
     9421 gatcggctta agtgtagcaa aacaagccaa agaagtacgg ggaatggata ccgttcctca
     9481 agcaatcgct gatgcaaaaa aaaatgcgga aagattagga tatcacaaca ctcgatatga
     9541 agtcgggaca gcagagcaag ttctgcctaa atggttgaat gaaggttttc agcctgatgg
     9601 tatcatcgta gatccaccac gtacgggttt ggataacgca ttgatccaat cattattgaa
     9661 ataccctcca aaaaaacttg tttatatctc ctgtaatgtt tctacacttg ccagagatct
     9721 ggttaattta agtaaaaagt tcaagattga atatttacag tccgtcgaca tgtttcctca
     9781 gactgcacgt tgtgaagttg tcgtgaagat gacgagactc gctgactaaa tgaaagaggt
     9841 tgtgaaaaag tctgccctgt atcaagtaat aagaacagcc aaacaaaaat agctcttcat
     9901 gttttatgaa tactgtactt gtgaccgaag agttaacagg aggacactat ttatttaaaa
     9961 aaagggttgt gacatagttt ttgtcacagc cctttttgat tattattgga tattcacgtc
    10021 tatcacttga taaaatgcat ttgtcgtatc gctgatattc catactgcta aaatcacatt
    10081 atatcctttt cgatcctgcg gaagtgtgat cgtttggttg actgtttttg gtggaacagt
    10141 tgctttgtcg tctatttttg tgatcaattc aaaatcacta aatttcaaag gttgattagg
    10201 attccaagtt ggtttggtaa tgtagtaatc ccaagtactt gtccgatgtt gtgctgttaa
    10261 attccaagta atatttaatg ttcctggatt aaccatgttt ttatgccatc tgttagatgt
    10321 ctgttcatct aaaggctcaa agccgctgac tcctgcacta gcaatttttc catcaatgaa
    10381 tgtatttttg gtagcttcga tactttgcgg ttcccattgt gctcttccga cattttgatt
    10441 taagtttcct gctgctgatg ttcctaggta tgcacggctt cctggagaag taatataacc
    10501 atgcgcaaaa cctggaatgc taaaaccgaa caaccctaaa ctaactaacc caataaacaa
    10561 taacgtacta atctttttca tgtagaaacc tccttcattt gataaccaca ttgtaaacgc
    10621 tttattgtat taacacaaac attatgcttt tataatttta tgttgaagta aaaaaggtga
    10681 aaaaagtaag atgcagatta taacagaagt agacgagaaa agtacaaata gaaaacatga
    10741 gaatcaaatc atatgatctt tttcaaaaac atcattcata taaacaaatg ttcaactttt
    10801 taataacaac aattatcaat ttttttctta c
//
LOCUS       DANKHL010000097        10509 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000097 DANKHL010000000
VERSION     DANKHL010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.101"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            66..692
                     /locus_tag="PW101_001734"
     CDS             66..692
                     /locus_tag="PW101_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2514133.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            692..1156
                     /locus_tag="PW101_001735"
     CDS             692..1156
                     /locus_tag="PW101_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514134.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            1351..3660
                     /locus_tag="PW101_001736"
     CDS             1351..3660
                     /locus_tag="PW101_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL2514135.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            3672..4376
                     /locus_tag="PW101_001737"
     CDS             3672..4376
                     /locus_tag="PW101_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2514136.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            4373..7132
                     /locus_tag="PW101_001738"
     CDS             4373..7132
                     /locus_tag="PW101_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2514137.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWMDVVQPTTVDGFKACTDPRQAAYAFERNYER
                     PAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSG
                     YLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINW
                     ARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSI
                     VIPPK"
     gene            7145..8053
                     /locus_tag="PW101_001739"
     CDS             7145..8053
                     /locus_tag="PW101_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HDL2514138.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            8053..8670
                     /locus_tag="PW101_001740"
     CDS             8053..8670
                     /locus_tag="PW101_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514139.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            8674..9123
                     /locus_tag="PW101_001741"
     CDS             8674..9123
                     /locus_tag="PW101_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514140.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTMVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            9123..9617
                     /locus_tag="PW101_001742"
     CDS             9123..9617
                     /locus_tag="PW101_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514141.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            9631..10062
                     /locus_tag="PW101_001743"
     CDS             9631..10062
                     /locus_tag="PW101_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514142.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            10064..10201
                     /locus_tag="PW101_001744"
     CDS             10064..10201
                     /locus_tag="PW101_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL2514143.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            10238..>10509
                     /locus_tag="PW101_001745"
     CDS             10238..>10509
                     /locus_tag="PW101_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514144.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT         3653 a         1589 c         2332 g         2935 t
ORIGIN      
        1 acaaagaaaa aattgaagaa attatgacta gaaaaatact tgatttatgg gaagggtgat
       61 tttaattggg aaaacaagat actttttatt ttgaaggatt ggacgacatt ttaattgcga
      121 tgatggcaac gccagattct gttggaactg caccgactta tagtgaagtt ttacgattgc
      181 caattgccac aaaaatcggt gtcaaaggga atggaacagc attagaaaaa tgggcatcca
      241 gcaaaatgtt tcgtcgtgtg tcgcgagaaa caaaacatga acttgcgttg gatcacgtgg
      301 gtattccgat agaagtgatg gatgaaatca aaggattagt tgctaaaagc ggagtaacgt
      361 ttagcaaaaa taccgcacga gaatttccgt acttcgcctt tggatttatt ggaaatattg
      421 aaaatggcgg taaaaaagca gtttggtatc ctaatacaca actatccaat gtgattgacg
      481 aagagtatgc cactgcagag gaagaaacaa aaattgacga tgtaactgca aatctggttt
      541 ccatcggact gaaatacaat aatgtgatgt atgcaagttt tgattcaaat agagacggag
      601 cttctctaga attatttaat aaattcattg ctcaacctgt atacgatgaa gaacagtgga
      661 aaacattggc taaagttgga ggtgcgggtt aatggctcgg ttatctgatt atggaattca
      721 cgttgaagac ttaaaaaatt ctgctgttgt aattattcaa ggcgcagaat tccctatttc
      781 atttactatg caaacgatgg aatttatagc agatgtgtat ggtggagatt attcgcaatt
      841 tgaatctgat atgaacgcca tgctatctaa aaaagaagga caaatttctt ctgctaactt
      901 atcgcctagt gatctaaaaa tcatgcgtgc cttgatttat gccatgctgc gtactggtgg
      961 tttagaggaa gatccagaaa ctattttcaa atttttggga atgagtggag aggtgttgtc
     1021 tgcttatagt acctgtatgg aaatttttgc tagccagaca tttcaggtgg aagacctaaa
     1081 aaaatccaag aagccacaag actttcaaaa agcgcaagca aaaagaaaga aaaacaaaaa
     1141 gaatcggaag agataggaac tccttggagt ttttatattt acgttgctct cactctattg
     1201 aattggagtg agagtttctt tttaaagtct acacctaact tgtggctcaa atcttattta
     1261 cagtggttac aacaaaacac cgattttgaa ccacctcaat ctgtaactat ggataaatcg
     1321 ccttggtggt agaaaggagc gctaagataa atggctggta aagaatctga tgtcgttctt
     1381 aattttaaaa cgaatggcga agtcagttat tcgaaaacaa tcaaagaaat caacaaagaa
     1441 atgaacttag ccgctgccga gtacaaaaac caagtgtctg cgatggacaa ggatgcaact
     1501 caaacagaaa aattgcgagc ggctaaacaa aaattagaaa aacaattagg attggctgaa
     1561 caacgatctc aaatgctgag agaggaatat gaaaaatctg tcaaagaaac cggtgaatat
     1621 tctgctgaat cagaaaaact ttataaacaa ttactcaatt ctgaaaccgg agaaaataaa
     1681 cttcgtacag cattagaaca gacgaacgat gcacttaaag aacaaggtga cgtttctgtt
     1741 gatacagcaa aaaaactcca gaaaatcgaa gaaacaggtg agaaagtaaa aggcgttggt
     1801 gaaaaaatgt ctgttggagt aaccgcgcct attgtagcgg caggagcagc aggacttgca
     1861 gcatttggtg aagttgacga ggcacttgat accatcatta caaaaaccgg agcaacaggt
     1921 gatcaagctg atagactttc acagtctttc aaaaacgttg gttcaaatac tcatttacct
     1981 ttacaaacgg ttggggaagc tattggtgag gtaaatacac aatttggatt catggataaa
     2041 aaactggaag attcaaccaa ttatctccta cagtacgctg aaatcaatga tacagatgtt
     2101 tcgcaatcag caatatctgc tcgacaagct attgatgctt atggactaga atatgatgat
     2161 ttgaattctg tccttgatgt aacaacgaaa acatcgcaga atactggtca atctgtagac
     2221 gacttgatgc aaaaagcaat tgatggcgca ccacaaatta aacaattagg gttgagcttt
     2281 ggtgaagggg tcactttact tggacaattt gagcaatctg gtgttgattc aagcgcagct
     2341 ttaagtagtt tatctaaagc aacagtggct tatgcgaaag acggaaaatc tttaagtcaa
     2401 ggactagaag aactacaaga caaggtaaaa aatgcaggtt ctgaaacaga agccatcaat
     2461 gcagcagcag aagtattcgg gactaaaggc ggtcctagaa tggccgatgc aattcgtaga
     2521 ggtactttga acctggaaga tttagcaaaa actgctggag aaagtggggg agctgtagga
     2581 gacacattcg atgctactct cgacccaatt gatcaagccg atcaagctat gaacaatgca
     2641 aaattagcaa tggctgacgt aggtgaagca gtccaagtaa gccttttgcc tttttttgaa
     2701 aaagcaactt ccttattaca ggaattctct aaatggtggg ggtctctaga tcaagataca
     2761 aagaattgga tcataacaat tgctggtata gcggcagcag ttggaccagt actaatcgtt
     2821 cttggttcac ttatgggatc tgttacaaaa atcgttgggg aagttaaaag ttttatagga
     2881 gtttggcaag gacttgctgg tttatttgga atgtcgggag gctggtttgc attagcagtt
     2941 atagctattg gcgcattagt tactggacta atttgggcat ataataacgt taaatggttt
     3001 catgatggag tgaattcttt cttcaaaggt gtttcagatg tagctgttga aatatttaat
     3061 tttttaagtg gttattttgg tggatttttc gatggtgtta tagcaaattt caataatttt
     3121 tataatgctg gaaaacgaat tttcagtggt tttctagatt ttatcactgg agtatttaca
     3181 gggaattggt caaaagcatg gcaaggtcta gttgatattt ttggaggtat tttcgatggt
     3241 attgttgctg tagggaaagc tcctattaat gccatgattg gattgatcaa tggatttatt
     3301 ggtggattga ataatataaa gataccaaaa tgggttcctg gtgtaggtgg aaaatcattt
     3361 tctatttcaa aactgcctta tttagcacaa ggcggtcatc taatcaatgg acaagcgatt
     3421 gttggtgaag ctggacctga gttactgact gccaagaatg gtaaaaccac agttactcca
     3481 ttatcagacg aagaaaaacg caaagggatc agtggaagag tttctggagg tagcattgaa
     3541 cagcatatac acattggcaa agtcgatgca aacaatccat ctgagttaga tcgtatgaat
     3601 cgtaaatttg ctaaagcaaa caaacaggct attcgtgatt tgggaggtgt tcctatttga
     3661 gtcgacaatt tatgaatcca gatgaaccaa attttatttg gaaaaatcgg aatgcagtaa
     3721 ttgatatgaa ttgcatcatt gaggcagagc ttccagatat tatgccgaac aagcgatacg
     3781 aaacttacac gattcagggc cgaagcggag aattgacgga gacgtttgat gactatgaac
     3841 cttttgattt ggaaatagaa gggatcacta ttccacattc gaaactgaga gaagtcaaac
     3901 gatggctcac tggtaaaagc caattaatca cacataatga tccagacaag tatctagagg
     3961 ctatctgcag catggataaa gaagtccctt tcgaaaacga gtggggcttc ttttatacat
     4021 tcgatgttac ttttcgttgt cagccactca aaagaaaaat cggtgaagca cctaaaattt
     4081 ttcattctag tgttatggat atttttgatc atggtgacga aattgcacat ccctatctgg
     4141 aaatagaatc gaacggagga gatataacga tcgctgtcgg tgataaaagc ctaacaatac
     4201 ttaatacgtt agctgcaaca atcgtggttg atactcagct tggaaaagca atacaagaag
     4261 gtttgaattt atttacgcgt ggtgattggc cagtattgca gcctgaatgg aatcaggtaa
     4321 aaatatccgg aaaatttaaa gaagtgcggt tttggaatag gagtgtgtat ctgtgacgca
     4381 agaatttatt tatgcttata aaaaaatgcc agatgatttg agcgtgaacg gagcgtcgtt
     4441 agttgattgg gaagatttgc cagaaatcaa tcgtgtgctg aatggccaat atcgttttta
     4501 tggtaactat tcaagaagcg gacagtatcg ctcttattta aaaaaaggaa attttataaa
     4561 agcgaaggta ccagatggat catggcaata ttttgaaatt tataatgtca aaaagaattt
     4621 gacctccgtt tctgtaacag ctcggcatat tggttttatg gccaataaga actttattgt
     4681 caagtcgttt acggataacg gcaatgggtc tcagatcatg accaatctta aaaacagttt
     4741 ggcatttgat caaaaattta actatctttc gaatgtaggg acaacgcacc aatttacagc
     4801 aagacaagtc gcgccagttg aagcaatcat tggttcaaat aacggtaatc aaaatttgac
     4861 tggcgtgact agcgcggaac ttgatatgga taactatgat ttgaaactgg taaaacagat
     4921 tggatcagat aatggttttc ggattgattt tggcatcaat ttagaagcaa tcgaagaaga
     4981 aattgatgaa gaatcaattg tcaatagttt gtatttggtt ggcggtgttc ctgataacga
     5041 ttatgatgaa gacaaagaac ctatcgaata tggttattta gaaattgatg gagtaaccaa
     5101 cgagaatcga cgtattgcca aacgtgaaaa cggagattgt aaaacagttg atgaattgat
     5161 caaatggggc aaaaccttat ttgacaatga tcgtatccat gagccaaaag ctactcatac
     5221 agtcagtatg gtggcgttgg aacacacact cgaatacggt gacatgtatc aagagctagc
     5281 gtctttacac tttggtgatg tcgctcatgt tagggcgaaa gagctagata tcgagataaa
     5341 agagcgtatg gtcgaataca cttatttccc aacgcttggg aaatataagg atattgtttt
     5401 aggtaatgat ttatcactgt acacttcggc agtaaatact caagcacaag aattgaaaaa
     5461 gaaaatcgac aatcgaacag aaacgttagt tcaaaatgtg ttgaatgcga cggcatggat
     5521 cactggtaat tctggtgggc atgtcgtttt tcgtccagaa aaagctccat cagagattct
     5581 tatcatggat actccaaatg tggcgaatgc aaagcgagtg tggcgttgga atttaaatgg
     5641 cttaggttat tcagacaacg gagtaaatgg cccgttcggt attgctatca catcaaaagg
     5701 agaaattgtt gctgacttca tcaaagtagg aacaataaat gcagaagtat tcgaaacttc
     5761 ctttaatgct tatggtgatg tgttaaaact tgtaaaaggc acgcttcaaa tttggaatga
     5821 aaacaaaaaa atcatggagc taaccaaaaa aggtatggaa ttctggaata gaaaagaatc
     5881 cattggaacg atcggaacaa cagattcagc aggaaatcca tttccaaatg ctgtaacacc
     5941 tacacctctt gaggaaaatt ctttggtaat acgtacaaat ggcgatggaa aatacatttt
     6001 aatttctcca actgcagaaa aaggatttgt gttattagga aacggaaaag catattattt
     6061 cggtgattta gatattcagg gaaaacttac tgttagaggt aaagaagtga taccaggaca
     6121 aaatggcggt ccatctggcg gaggagaaac acctggtggg tatcctgatg aattaaaaac
     6181 agatgcagaa aagagagctt ggagaatata cgatattttg tgtaataacg gatttacgaa
     6241 acaatctgca tgtggaatat tggggaacat tcaacaagaa acaggaggaa cttttgatcc
     6301 tgatactgtt caaataggtg gaccagcata cggattagtt cagtgggatg gttcttcata
     6361 tcctcttgtt ggtccagcaa catgggatgg aaaagtttat gttcaaaact tatttaatgc
     6421 tgcaggtatt aaagaaccaa taacgagctt agatgcacaa gttcgattgc ttatttggac
     6481 atttacaaat ggacaatgga tggacgtagt acaacctacg acggttgatg gatttaaggc
     6541 ctgtactgat ccaagacaag cagcatatgc ttttgaacga aactatgaac gtccggcagc
     6601 gacacatcct gaacgtcaag attatgcagt taactggtat aacaaattta aagatttaaa
     6661 accaggagga gctactggag aagcaggact aaaacattta gagtctttga ttggacaacg
     6721 aattggcaat ggtcagtgtt atggcttgtc tgcagaatat tcaggatatc tgggtggttg
     6781 tggcatgggt gctggaacaa aatatggttt aactcatgtg ataggaaata cttctgcagc
     6841 atctgatatt ggtattgcct atgattggtc tgctgttggt tggaaagtga ttcaaaaccc
     6901 tagatatgat caattagtag ttggtgcaat tattaattgg gcaagaggtg gacaagtggg
     6961 ttcatggttt gcagatggaa cttatggaca tactggcgtt atccgaggct tagctaatgg
     7021 tcgtatgcaa acttatgaac aaaacacaga gttaggtatg atttgtggaa agttagatcg
     7081 acagtattat agtgcaagtg caatttcttc cattgtcata ccaccaaaat agggaggtga
     7141 tttaatggca aagtggaatg tcatattaag tacaacagaa ccctataatt acgtggggat
     7201 gattcaagtt cgacaaggca ataaaaacac tgaagttatg gaagctacaa ttgtcgaaaa
     7261 tggtcttcca tatgatttat cagaatgtaa ggtatatttt gaatcagttg ttggcggaaa
     7321 atatccagtc caattagaaa caaaaattgt ggatgctaaa aaagggaaaa tcaaatatat
     7381 ttttgataaa tattccatgc agtgtttgca tcgacaaaca gccaatttca ttatatttaa
     7441 aggagaagac ttgattggaa caactcaaga cttctcttat tttgtcatca atgctgtttc
     7501 aaaaacagaa ggagaaatgg gttcttattg gcaatcaatt gaagatttaa ttgctgatat
     7561 gacagatttt attaatgaaa ataaaggcga ttttacggac tggatgaatg aaaggaaaga
     7621 agaatttgat cgctggagag aggaacagga gaatagtttc caagattgga gagaaggaca
     7681 agaatcagat tacttgtcat ggtttgaatc aatcaaggat attttgaagt ctatcgatcc
     7741 aggtggagta atgttagccg aattaatgga tgcacgtgta gatcttcaag gtgtacgcca
     7801 tgaatcgatt tctgaacgtt tcctatccga tctaaattat ttgtatcaaa aaatgaaagc
     7861 agcacttttc acgattgaat acggtgaaat tgaagtgact gatattcttc aagacgatct
     7921 cttttcagat aatcacgaag tcgaaaaaat tgaaacagta gaatttccaa ttgaagaagg
     7981 agctttgatt attgcaactg ttgatgatcc aaaacagaat gttttcactc ttgagaaagt
     8041 tggggtgatt tgatggttaa agtaaagaga atgatggaaa ctgatgaatc tggtgttaaa
     8101 cgtcaattta tgccaattac acatgtctct gcagttttag gcttagaaaa aatgatatca
     8161 ggacaatcaa aagttctttc ggttaatggt aaatatggtg cagtcatttt aacgaaagct
     8221 gatttaggat tagagaatgc aattactgaa ctaccttatg caagcgagac aagcgatggt
     8281 attcttactg ctgaaatgtt tcaaaagatc gtaaatggag aaggtggaac atatattttg
     8341 ccgattgcta ctcctgaaca attaggaggt ataaaagttg gggaactctt agaagttact
     8401 gaagaaggtg ttttatctgc aactaagcaa acagacttca atttctctga agaattaaaa
     8461 ggaaaattag aatctctaaa aatattaaaa gcaggtgcaa atatatcaat tgcagaagat
     8521 ggaacaatta gttctacagg tggtagtggg actggtggtg ttaatcagtc atatgtagat
     8581 caaaaattcc aagaagctgt aaatcaagca gaaaattaca caaacgaaag aattccaaat
     8641 tttacttttg aaaaaattgg ggaggtatag agaatgacag atatcgtaaa aataaaacaa
     8701 agcaatgtac aggtttatcc acaaactcat tggaatgcta tagaaggcaa accaacaatg
     8761 gtgaagggag ataaaggcga tcctggccaa gcagcaacaa ttaccatagg aaccgtttct
     8821 agtggttcaa ccgcttctgt tacgaatgta ggaacatcat ctgcagctag atttaatttt
     8881 gttttaccaa agggagataa aggtgatcct ggaataaatg ccacaactac agctgtagct
     8941 acaactacag caaatgggtt gatgtcgtcc acagataaaa ctaagttgga tggaattgca
     9001 gctggagcac agaaaaatcc aggtaatgct acaactacga cagcaggttt aatgtcagca
     9061 accgataaag tgaaacttga tggattagcg aatattacat ttgagaaagt ggggacggtt
     9121 taatggctga tattgttcaa ttaaaagaag acggagttgc taaatactta aaaacgcatg
     9181 tagaagctat tgatggtaaa gaagcattgg tgcagacaga tggagaccaa gccattgcag
     9241 gacataagaa tttttcaggt tctgtaacaa tcaataataa acgtgttttg acgactgatg
     9301 ataacagata tgaagtggta aatctggttg tcactaacgg taatacaggg acagcaaagc
     9361 tttatcgtga aggaaaaaca gtttctatat attttgtggc tttaaacgga aaaagtagtg
     9421 gcgggaatga ttcggttatt ttaactgttc cagaaggcta tcggacacca attagttttg
     9481 aacaactggt tgggtcaata gaccgttcta ctttgaacag tgctcaacta tctattggcg
     9541 cagacggagc cattaaatgg agaagaaatt caagttatgg atcagcttat tcatttgtta
     9601 tcacttattc aatttaaggg aggaaatcta atgaaagtag tttacaaatc aatcaagcct
     9661 tacggattcg agcaaatcat tttgaacaat caagaaaata tccctgaaaa ctgtacagag
     9721 attaaaccac cagttcctaa ctggagacca agatttgatt ttgataaaaa acagtgggtt
     9781 gagttagcta ctgaagaaga gaaaaaaggg aatgctgttg atagcgtaga tgaattggct
     9841 aatattaagg cattatacga aacgctgaag gcagaaaatg atgaattgaa acagctaaat
     9901 tctaaagcaa tgctaaacaa tgtagcaatc aagcaagaaa atgtcttatt gaaagaaaag
     9961 tcagaaagtt tagcgcagtt gaattcaaaa acgatgcttg cttctgtaca aaataccaag
    10021 gaaatcgaag aaatcaaaaa acaattacaa ggtgggaagt aacatgtatt catatgaaga
    10081 tatcaaacta atgtatgact ggggctgttt cactaacgaa caagtaatgg ttttcgttcc
    10141 attatgcatt acagacgaag aagcagataa aatcattaat aaagataaga gcgcatctta
    10201 attgatgcgt ttttatttaa ggtaaaggag ttgtcacatg attaatttag gggaatgggg
    10261 aatgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa ttagaccgat
    10321 tgctgcatct ttctcgaaaa ttactgagac tctttcaaaa gtaagccaca atttagattt
    10381 gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg aagaactaaa
    10441 gaaacacgat gaaagattag atacacatac agaaaaattg gtggaacaca cgcaacaaat
    10501 caaaacttt
//
LOCUS       DANKHL010000098        10488 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000098 DANKHL010000000
VERSION     DANKHL010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.102"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            23..646
                     /locus_tag="PW101_001746"
     CDS             23..646
                     /locus_tag="PW101_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514145.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="PW101_001747"
     CDS             1096..2145
                     /locus_tag="PW101_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2514146.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="PW101_001748"
     CDS             2142..3023
                     /locus_tag="PW101_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2514147.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="PW101_001749"
     CDS             3026..3796
                     /locus_tag="PW101_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2514148.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="PW101_001750"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="PW101_001750"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL2514149.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="PW101_001751"
     CDS             5107..6270
                     /locus_tag="PW101_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2514150.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="PW101_001752"
     CDS             6387..6797
                     /locus_tag="PW101_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2514151.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="PW101_001753"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="PW101_001753"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2514152.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="PW101_001754"
     CDS             9370..9654
                     /locus_tag="PW101_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514153.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="PW101_001755"
     CDS             9814..10050
                     /locus_tag="PW101_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2514154.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="PW101_001756"
     CDS             10047..10445
                     /locus_tag="PW101_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL2514155.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3652 a         1766 c         2224 g         2846 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcatt
//
LOCUS       DANKHL010000099        10404 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000099 DANKHL010000000
VERSION     DANKHL010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.103"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(372..1958)
                     /locus_tag="PW101_001757"
     CDS             complement(372..1958)
                     /locus_tag="PW101_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDL2514156.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            2209..2433
                     /locus_tag="PW101_001758"
     CDS             2209..2433
                     /locus_tag="PW101_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDL2514157.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            2575..4326
                     /locus_tag="PW101_001759"
     CDS             2575..4326
                     /locus_tag="PW101_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2514158.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            4326..6110
                     /locus_tag="PW101_001760"
     CDS             4326..6110
                     /locus_tag="PW101_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2514159.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            6568..7419
                     /locus_tag="PW101_001761"
     CDS             6568..7419
                     /locus_tag="PW101_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2514160.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            7516..9402
                     /locus_tag="PW101_001762"
     CDS             7516..9402
                     /locus_tag="PW101_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2514161.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(9443..10345)
                     /locus_tag="PW101_001763"
     CDS             complement(9443..10345)
                     /locus_tag="PW101_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2514162.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
BASE COUNT         3279 a         1866 c         2191 g         3068 t
ORIGIN      
        1 ttgattttgt cgctttcgtt tatgagctat ttttcttaca ccttttactt gactaatcgt
       61 aggaaaggga actgtgacaa aagtcttgtc gcagttcctt tttccgaata aacggtgctc
      121 aaaagcttgc atctgcggta ataagtccaa ccaagttaaa aacataaaaa gctgttttta
      181 cttggctgtt cttattactt gatgcaaggc agctttttca caacctctga tacacggtgt
      241 tccagaagta acttcttcgg aaataagccg aaatttcaca aaaatttgaa aagcaatttt
      301 cttaaattcc ttcttatttc ttgaagctgc ccacttctga cacaacctcc tttgtgtaaa
      361 agaatcagtt tctactcgat ttcttctatc gtttcctgca aacgctgttc atattttttg
      421 tttcctttat ataaatcacc aatcgtccat gccaacagga gaaggacaag aacaatgatt
      481 acataagcga tttgatcagc caatgctagt tgagctgagg tggcttgctc gccgaagaaa
      541 ctttcgattt gacctggtaa tcctcgtaaa ttcaagtaag tcagactaat cacagagaac
      601 cagccaagta gttttacaac aaatgaattt ttgaatcggc tccccatctc cgcttggcta
      661 ttggtcatca tcagtaaagg aatcatcgag aatggcaatg caaaagccag aaaaacttgg
      721 gattcattca tcaagttatt caaagcggta tgttcttcga tcgttccttt tgtacttgta
      781 aacaaaacac aaatcaatac aggaatcaca gaaagcaatc gtgtgactag tcttcttagc
      841 caaattggga tacgcatatg gatgaagcct tccatgatga cttgaccggt caacgtacca
      901 gtaatagtag agttctggcc agaagcaaga agtgctacag caaataaagt agacaagata
      961 ccagattttg ctacagcaat caagatacca ttgctcatag ctgatggatc agataaagct
     1021 tcatacagac caaaaaagga aggatcttgg acagaaccgg ttttgaatac agccacaccc
     1081 atgatcaata gcagtgcatt gactagaaaa gccattgaca actggatatt agaatcccaa
     1141 gtagaaaagc gtaccgcttg tgctacatct tcttcgtctt ggtgatcgat cttccgagtt
     1201 tgagaaactg ctgaatgaag atataagtta tgcggcatga ctgttgcacc aataatccct
     1261 aaagctccag tcagcggagt ttcgccgccg atgaccggtg agtctgagaa tgtgtgactg
     1321 ttagggataa atccggcaaa caaactgtgc caatcagggt tagacaagcc aacttgatag
     1381 acgaaaacga ataaaatgac gaaaatcaag cagacaacca aagcttcgat tttacgaaac
     1441 ccgacttttg tcagcaataa aaggacgaaa acatctaaca cagtgataaa tacggcaatt
     1501 accaatggaa tatcaaaaag caggtagagg gcgattgctg caccaatgac ttctgcgatg
     1561 tctgttgcca taatggctaa ttcagtcaag atccataaaa tgacacccaa tgtcttactt
     1621 gtccgtgctc ggattgcttg ggccaaatcc atttgggtca cgattcctaa ttttgctgcc
     1681 atatattgta aaagcatagc aatcagacta gacatcaaaa taactgacat caataaatat
     1741 tggaaatttt gtccaccagt gatcgaagtg gaccagttac ctggatccat ataaccaact
     1801 gctactaatg cacctggtcc agagtaagca agaagtgttt taaaaaaact ttttcccttg
     1861 ggtacatcca ctgttccatt tacttcagct aaagataaac catttgtatg ttcaataagt
     1921 cgatgcttat gaaattgttt gttggattga ttattcaaga aaaaaccacc tttcctattt
     1981 atctacgatt acattctaca cctcagaaag aaaaaattaa agaaaaagat ttggtatgcc
     2041 taattttttt tcgtggcgtg tcttgctggt taatgaaaga aattttgttc ttctctaaaa
     2101 aaaccactat tttttgcatg aaactaaaga atacgccttt cttttttcaa taaactttag
     2161 taaaataaat cattgagagc aattcggaaa tagaaaggag agatttatat ggtatcaaca
     2221 ggtattgtcg tacttattgc tattattgct ttgctaatcg gcgcagtagg tggctttttc
     2281 ctagcgcgta aatatatgaa agactattta gaaaaaaatc ctcccgttaa tgaggacatg
     2341 ttgcgatcaa tgatgatgtc aatggggcaa aaaccatctg agaagaaaat tcgccagatg
     2401 atgcagcaaa tgaagaacca agggaaaaaa taggttctga cccaaagact ttttgcgggc
     2461 tgattttttc agtccgtttt ttatttcgta atttgttaga ctattcagac tattcagcaa
     2521 aaaagacaag cacagacaat cctatgctct tgataaagac aaaggaggag taaaatgtcg
     2581 atttttagaa aattaggttg gttttttcga caggaaaaga aacattattt aattgggatt
     2641 ttctccctat tcgcagtggc cttggtacag ttagctcctc caaaagtgat tgggatcatc
     2701 attgatgaga tcgccgataa aaatttatca ataaagatca tttcctggtg gatcttatta
     2761 ttgatccttg cagctctctt acaatatctt tttcgttata tctggcggac gaatatttgg
     2821 ggaagtgcag ctcggttgga gaaagaactt cggcagcagc tattcaaaca ttttacagaa
     2881 atggacaatc tcttttacca aaaatatcgg actggagatt tgatggctca tgcaacgaat
     2941 gatttgaatg caatccaaaa tgtagcagga gctggtatct tgacctttgc agattcagtg
     3001 atcacgggcg gaacaacgat cattgcgatg attttgttta ttgattggcg cttaacactg
     3061 attgcgctga ttcctctgcc tttactagcc gttacttcac gagtgctggg gtcaaaactc
     3121 cacgatgctt ttcgtgattc gcaagaagct ttttctagta tcaatgataa gacacaagaa
     3181 agtattactg ggatcaaagt cattaaaaca tttggacaag aaaaagaaga tctcgcggat
     3241 tttgaagaaa agattgaaga tgcaattatt aaaaacaaac gagtcaattt tttagactcc
     3301 ttatttgatc cattcattac cttgattatc ggtctgtctt atgttgcaac aattatttta
     3361 ggtggaaagt atgtcttgga aggcacgatc tcaatcgggc agatggtctc atttattagc
     3421 tatatcggta tgcttgtttg gccgatgttt gcgattggtc gattgttcaa cgtattggaa
     3481 agaggaaatg cttcttatga tcgcgtcaat gaacttttgc atgaaaagac gcatatcgtg
     3541 gaacaactgg gtgcagtgca gactccggca aaaggcgagc tgaatgtagc tatttcctct
     3601 tttaactatc cgaaagaaga atcaccagca ttagaacaaa tcaaatttgc gatccaagaa
     3661 ggagaaacat taggtattgt tgggaaaact ggtgcgggaa aaacgacaat catcaaatta
     3721 ctgatgcgtg aatacgatga ttatcaagga aaaattcaat ttggcggatg ttcgatcaaa
     3781 gaatattcat tgaatgcttt attaggatca atcggctatg tgcctcaaga tcacttcttg
     3841 ttttcgatga ccattcgaga taatatccgt ttttctaatc caaaactttc agaagaagaa
     3901 gtagagcatg cggcttcttt agcatttatc aacaatgaaa tcaaggaatt accaaaaggt
     3961 tatgatacct tagttggaga acgtggagtc tctttatctg gcggacagaa acaaaggatt
     4021 tcgattgctc gagcattgat caaaaatcct gaattgctga tcttagatga tgcactttct
     4081 gcagtagatg ccaaaacaga agaagctatc ttgcataatt taaaagaaat aagaaaagag
     4141 aaaacaacca tcattgcagc tcaccgttta agcagcgtga tgcatgcaaa agaaatcatt
     4201 gttgtcgatc aagggaaaat cattgaacga ggaacccatc atgagctgct tgcattaggc
     4261 ggctggtatg aacgaatgtg ggagaaacag aaattagaag cgaagataga aggaggcgag
     4321 gcctgatgga tgaaaattat caatcggaat ggacaaaatc gattccactt aaagaacaga
     4381 agaagatcat caaacgactg atgaagttcg ccaaaccttt tagaagaacg ttcatcgttg
     4441 ctatcttatt tgcgtttgcc ctgtctgtga tcaatgtcct attgccaagg atcatccaaa
     4501 cttttatgga tgatcatctg gctaaacagt cggctacgac acaagttatt ttgttttttg
     4561 ctggtatgta cttgtttgga gtcatagtca aaagtatcat ttggtttttc caatggtatt
     4621 tgtattccat ggcttcattg aagacctatc agtatgttcg ggtaagatta tttgaaaagc
     4681 tgcatacact aggtatgcgt tattttgatc agacaccggc aggatcgact gtttctcgag
     4741 tgacaaatga cacagaaacg ctatttgaat tttggtatgt ctttttgatg gtattgactg
     4801 ggatctttgc agtcgtttct tccttcattg ccatgtttca aatcaatgga gaaattgcct
     4861 tgtggacttt ggtgttcttg ccgattttag gaatcgtcat ctggtattac cagaaattca
     4921 gttctcgtat ttaccggaat atgcgggaaa aattaagtca gctgaacacg aaattgaatg
     4981 aatcgatttc cggcatgcgt attatccagc agtttcgcca agaagctcgt ttagctaaag
     5041 aatttgaaca agtgaacaac gagtatttag acacgcgcta tgcgatgatc aaaacaaatt
     5101 cgattctgtt aagtccgatc atcaattttc tatatgcatt ggcgattgcg ttggcgttga
     5161 ctttgtttgg gatcgatgcg ctgcattctc ccgtagaagt tggactgatc tatgcattta
     5221 cgacctatgt ccagggattt tttaatccaa tgtctcaaat gatggacttt ttgagcacct
     5281 ttacagatgg aatggttgcc ggtagtcgta ttttgaaaat catggataca gaagaatatg
     5341 caccgcaaca aaatcctgga gcagatcaag cgattacttc aggtaagatc gagtttcgca
     5401 atgtaagctt ttcttatgat ggagagcaca atgtattgaa caatatctct tttgtcgcta
     5461 atccgggaga gaccgttgca ttagtggggc acaccggaag cggaaaaagc tcgatcatca
     5521 acgtattgat gcggttttac gaattttatg aaggccagat cttgattgat gatcatgaca
     5581 ttcgagaata tccaatggaa gagttacgta agaaaatggg attggtcttg caagatgcct
     5641 tcttgtttta tggagatatc gcaggaaata tccggatgat gaatccaact atcacagacg
     5701 aacagattcg ccatgcagct gagtttgttc aagcaaacaa gttcatcgat gaattaccaa
     5761 aaggatatca ctcaaaagtg atcgaacacg gtgccagcta ttctagcgga caacgacagc
     5821 tgatcacttt tgcgcgaaca atcgtgacag atcctaaaat actagtattg gatgaagcga
     5881 cagcaaatat cgatactgaa acagaaggat tgatccaaga aggattgaca aaaatgcgac
     5941 aaggacggac aacgattgcg attgcccatc ggctgtccac gattcgggat gcagaattga
     6001 ttttagtttt agacaaaggt catatagtag aacgtgggaa tcatgaaaca cttctagcgc
     6061 aaaatggttt gtatgcagat atgtacaaac tgcaaagcag tggtgtgtaa aaagtggttg
     6121 tgacagggct ccgttcagct ttaggtgata agaaaaaaat tgtaaaaaag gctttccaga
     6181 tttttgcata aatttggctt atcaccgata agctggtact ggaacaccgt atattagaag
     6241 ttgggacagt agcatagtag cattttgacc taagcgctag agtgaaaata aaacagagct
     6301 tcccagattc ttcatctgtg agcgttagtg tcgtaattac cagatttaaa aaaaatcacg
     6361 acagctagtc agaagtgccc aatagccgct aagaaagcgt tgacaaaagt gcttgctttc
     6421 gataatataa agaaaacgat acaacttgga tagtgattgc ctgatgcaag ctaaacctga
     6481 tgagtctccc atggttccat ggacggcttt ttgggttttt ctttttgttt tggatacagc
     6541 cctatatatc atggaaagga gtaaaacatg ctgattgaga aagtactgaa taacaacgtc
     6601 gtaataacca gggatgaaag aaatcaggaa atgatcgtga tgggaaaagg tttggcattc
     6661 aaaaagaaag tgggtgaccc gatttttgac tcatgcatcg acaaaaaatt tcagttgaca
     6721 aatgacggac tttcacaaca gttccaagaa ttggcacaag cgattcctct tgagtactta
     6781 gaaatttctt gtgaaatcat tgaatataca aaagaggctc tacaagtcca gttgaatgat
     6841 tcggtgtata ttgcattgac ggatcatttg tatacggcta tcgaacgtca caaaatggga
     6901 atatttgtac caaatgtatt gctgtatgat atcaaacaac tatttcctaa agagtatgcc
     6961 atcgggaaaa aaacagtgaa gaaaatctcg gataaatacc acatcaagtt atcagaagat
     7021 gaagccggat ttatcgcatt gcatttagtc aatgcacaag gcattcaaag ccaacaagac
     7081 aatacctacc tgtttactac aacaatacag gatattctaa atatcgtccg ctattactat
     7141 aagattacat ttgatgaaga ttcagtccat ttttttcgat tcgtcacaca cctacgtttt
     7201 tttctgacac gcgtcctaga tggagaaaaa tcagaagtgg gagaagtttc tgaggaattg
     7261 taccaggtta ttcaaaaaaa atataaggat acgtttggtt gcgttgagaa aatagcagac
     7321 tttttagccg agatattcaa ctaccagatg tccacggacg aaaaaatgta tttaacgatc
     7381 catattgcgc gtttagtgca tgagaatcgt tgtaaataaa taaaaaaagg aggataaatt
     7441 aacaagaacg catgaaataa gggtagtgac attcagttgg ctaaaccttc cactaataaa
     7501 ttggagggaa tagaaatggg aaaatatcaa gaattagcga gcaaaattgt agaaaatgtc
     7561 ggcgggaagg aaaatatcaa tggtttgaca cattgtatca caaggcttcg tttcaaatta
     7621 aaaaacgaag aaaaagcaaa tgatgagatc ttgaaaaata tggacggtgt tgtgacggta
     7681 atgcgagcgg gaggacagta ccaagttgtc atcgggaatc atgtgccagt cgtgtttgaa
     7741 gaggttataa aagcaggaaa ccttactttt gatgaggctg tttcaacaga aaaaatgaga
     7801 ccgtttgata tgcttattga tggaatatcg ggctgtttcc agccattcct tgctattcta
     7861 gctgctggtg ggatgattcg tggactgaca gcattacttg tcttcttagg cgcatttgac
     7921 cgcggttctg gcacatttgt tatgtttgat aatatcggtg attctgtatt ccaatttatg
     7981 cctgttatta tcggtttaac agctgcccgt aaatttaaag taaatgaatt cgtagggatg
     8041 ttgcttgggg cagcattaat gaatcctagc ttgtcgctgg aagctttgag tggtgcagca
     8101 gaagcaccac tgactaccat attttctggc actatttttg aagcacctat ttatcaaaca
     8161 gtattcggca ttccctggat tgcgcgtaac tacgcttctt ctgtcattcc cattatcttt
     8221 attgtgttgt tagctagcca aattcagaaa ccaattaaaa agttagtacc tgagatgatc
     8281 gcgaatttct tcgttccatt cttcaccgtt cttattacaa tgcccttagg ttttttactc
     8341 gttggaccag cattcacatt cgcaactgat attttaatgg ctggatttga aacactttta
     8401 gcactttcgc cagttattta tggagcaatc gtaggcttct tctggcaaat tctagtgatg
     8461 tttggtctcc attgggcaat cgtaccaatg ggattgatgc aattttctgt gaatggttgg
     8521 caaaatatca tgacacctgt tgcagtagtg tcttttggac aaactgcagc acttactgca
     8581 ctctacttta agcttagaaa cccaaaagac aaagcaatcg caataccagc cattgtttct
     8641 ggtattgtgg ggattacaga gccagctatt tatgggttta cattgccacg taaaaaaata
     8701 tttattttta cctgtgtcgg tggtgcgtta gggggcgcgt atagtggatt aatgaattta
     8761 acttcttgga atccaggtgg attaggcatc tttacaattc ccaactatat tagaccagat
     8821 ggtgatttaa cggatgtgat taatgtattg attggaatcg ccattgcgat ggtggtatcc
     8881 tttgcgctga cttttttctt ttggaaggag gaagcaggtg aaacagatga tagacagaaa
     8941 agatcaggta aagaaattgt aaaaacaccg atacaaggac aaatcgctcc actgaatgca
     9001 gcaaaggatg cagcttttgc acaaggaaca ctaggtcgag gaatcttgat ctaccctgaa
     9061 aaaggagaag tacgtgcccc ttttgacgga acagtcatga ccctgttccc gacgaaacat
     9121 gcaatcggaa tggtttctga agcaggtctg gaattgctga ttcatgtcgg attggatact
     9181 gttcagttgg aagggaaata ttttgaaagc ttggtgcagc aaggagaaag agtgaaacaa
     9241 ggcgatctgt tgctccgctt cgacaaaaag aaaatcgaag aggaaggata cagtttagaa
     9301 acacctgtga tcgtcaccaa ttacacggat tatttggata ttttggaaaa tcaaggggag
     9361 acggtcgggc cagacgatgc acttatcacc gcactgacct gaacggttag gtgaaagaat
     9421 ggactaggct taattattct ttttagtcca cttctttatt ttcaaaattg aaaggctctt
     9481 tcatgatggc tgagctgata gggcgtttat caaatttttg tcctacagaa attagatgga
     9541 ctaaattctg ttgataattt tctgcaaaaa cacaactata taacacgtct agaagataac
     9601 aaatcgatgt attgatcgta aaattaccaa ttttcgaata cagtcgttcg cgtgtcgtaa
     9661 gtcgcaaagc ggctgtactc attgaagata gcgttgaatc accgatactg gtcaaagcta
     9721 aaattggaac atgattttgt ttcaatattt ctgcggtacg aaggatcaca ttgttttcac
     9781 ccgtataaga aatcagaatc ccgcacatat cactcgtgca attgtacgct tcatagaacg
     9841 attcaccaaa agtggtgaca atcgtgacat ccattttgat gcgcttcatt ttcagcatga
     9901 aatcttgaga aatcaaagta ttcgagttac tggcgaaaat cacgattttt tttgcttgaa
     9961 gcattagttg tttggctttt tgcagttggt catgatgaat gagagaaagt gtatcttgaa
    10021 tcgtcatctg ttctaaagaa gccattttat ttgcgatcgt cataataccg tctgtggtgg
    10081 aaaaaggaag attcgcatct acgtcttgaa aattcccttc aagataggct gtttcagcta
    10141 aaaaagcatt acgaaaactg acccagccat caaaacccat tttttttgct acacggatga
    10201 gtgtggacgg gtggacataa catcgctcag caatctcttg gatggtcatt tcgccaagtg
    10261 ctggagaatc gcttaagaga aactgaatca cttcattttc tgaaggagaa aaggagattt
    10321 ctttgatttt ttcacgtaat aacatgtaga taccttcttc ttgttttttt acgtaaatta
    10381 taactttttt gaaaatgatt tgta
//
LOCUS       DANKHL010000100        10212 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000100 DANKHL010000000
VERSION     DANKHL010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.104"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            135..284
                     /locus_tag="PW101_001764"
     CDS             135..284
                     /locus_tag="PW101_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDL2514163.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            295..1809
                     /gene="dltA"
                     /locus_tag="PW101_001765"
     CDS             295..1809
                     /gene="dltA"
                     /locus_tag="PW101_001765"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDL2514164.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1806..3011
                     /gene="dltB"
                     /locus_tag="PW101_001766"
     CDS             1806..3011
                     /gene="dltB"
                     /locus_tag="PW101_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDL2514165.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3056..3289
                     /gene="dltC"
                     /locus_tag="PW101_001767"
     CDS             3056..3289
                     /gene="dltC"
                     /locus_tag="PW101_001767"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDL2514166.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            3292..4560
                     /gene="dltD"
                     /locus_tag="PW101_001768"
     CDS             3292..4560
                     /gene="dltD"
                     /locus_tag="PW101_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDL2514167.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4701..5783
                     /locus_tag="PW101_001769"
     CDS             4701..5783
                     /locus_tag="PW101_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDL2514168.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5783..6586
                     /locus_tag="PW101_001770"
     CDS             5783..6586
                     /locus_tag="PW101_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL2514169.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6656..7396
                     /locus_tag="PW101_001771"
     CDS             6656..7396
                     /locus_tag="PW101_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2514170.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7407..8411
                     /locus_tag="PW101_001772"
     CDS             7407..8411
                     /locus_tag="PW101_001772"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDL2514171.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8456..10153)
                     /locus_tag="PW101_001773"
     CDS             complement(8456..10153)
                     /locus_tag="PW101_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514172.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3270 a         1694 c         2074 g         3174 t
ORIGIN      
        1 atttgatgtg ctatttttat aaaagctatg tgacaaaact gttagtaaaa aaacggtttc
       61 tgtaagctag acacatgttt ctttttgtca aaattagtta cactttgtgt gaggttatat
      121 gaggagggaa tccaatgaac aaatggcgtt ctaatccaaa attgaattat tggggaaaat
      181 tcatcattcg aacaattttt tatagtgcag tactgctgtt cctgatttat ctgtatcact
      241 ataaaaatat tcaaggagga acttttatct ataacgaatt ttaggaggaa gagtatggaa
      301 atcaaaacga ttattgaagc aatcgatgat tggggaataa atgaacctga tcgagttgct
      361 tatcaggcgg atgagacgca tacttttggt gaattgaaac aagcatcaga cgcacttgct
      421 tattatttag aaaaaaaggt agaaggcgat ggaccaattg tcgtttttgg caatttggag
      481 tttgaaatga ttgtctcatt tctaggggtt gtcaaatctg gccatgccta tttgccgatt
      541 gaagagcata cacccaagga gcgtatccta tcgatctttc gggtggctaa accatctatg
      601 gtcatcagta tcggggactg gatagaagat attccttcgg ttcctgtcat aacaaaaaaa
      661 gaatttcaag aaatcaagaa gataccagta ggattttatc ccagaaatag cgtaaaaggt
      721 acagaaaatt tttatattat ttttacttct ggtactactg gagaaccaaa aggcgtacaa
      781 atcagtcatg ataatctagt cagctttgtt caatggacga tggaagactt tgggatcaag
      841 cctggtatgc atttcttagc ccaagcgcca ttttcttttg atttatcggt attttctatt
      901 tatcctgcat tagtttctgg cggaatgctg aagccgttaa ataaagcagt tgttcaagac
      961 tttcgtcaat tattcgcgac attgccagcc ttgaagttga atgtgtgggt ttctactcca
     1021 tcatttatgg atatttgctt gatggaacct acattcaatg gagaaaatgt gccggaattg
     1081 gacatgtttt tattttgcgg agaagaattg acgaaaaaaa ctgcagaaag tctacttgat
     1141 cgattcccaa atgcacggat atataatact tacggtccaa cagaagcaac tgtagctatt
     1201 tcaagtattc agattgacca gcatgtacta gatagttatg accgactacc gattggctac
     1261 gtaaaaaaag acacgaaagt ttctattgtc caagaaggta aacctgtttc aaaaggagaa
     1321 acgggtgaaa tcattattgc aggaccaagc gtatcaaaag gttacctgaa caatcccgaa
     1381 aagacagctg ctgcgttctt ttgtcaaaat gacttagcaa gttaccatac aggggatgca
     1441 ggtagactag atgaatatgg gcttttattt tatgaaggac gtatggatca gcaggtcaaa
     1501 ttacatggat accggattga actcggggat atcgaacatt atctgctcca agacaaccga
     1561 atcaaacaag ctgtcgtcgt accaaaatac caaggaacaa aagtacagca gctggttgct
     1621 tttgtcgtat tagaaaacaa aacgaaacag ccggattttc agttgactaa atctatcaaa
     1681 gaacaattac tagaagttgt aatggattat atgattcctc agaagttcca ttatgtcgat
     1741 ctgctgcctc agacagttaa tggaaaaatc gatcgaaaaa aattaacagc tgaggtgaat
     1801 cctgaatgat gttacctcat atgatccctt atgcagagcc gttttatttt tttcttttag
     1861 ccattgcgct tgtaccgatc gttctatctt tattgatatg ggaaaaaaga ttaccagttt
     1921 atcaatcagc agtgacactt ttctttttat ttgtcagctt tggaggtaat tattggcaac
     1981 agggagcggc gctgattggg tatgttttgt ggcagacgat acttgtatgg ggctattttg
     2041 tttatcggca aaggaaaaat cagacaggat ggttttttgc cgccgttatt ttagcgataa
     2101 ttccactgtt tttaacgaaa gtgactcctt tttttgaaca tggaaaagca tcgttacttg
     2161 gttttttagg aatctcttat ttaaccttta aatctgtaca aatgatcatg gaaataagag
     2221 atggcatgat caaaacatat aagccatttc gttatatcca gtttctgctg ttctttccaa
     2281 caatctcttc tggaccaatc gatcgctata ggagatttga aaaagatcag ctccatccgc
     2341 ctgttcgtga taaatatgtt gatcttttgg gaaaaggcgt ccaaaatatt tttgtcggtt
     2401 ttttatataa attcattatc ggttattatt ttggtcaagt actgcttcct gtagtgggac
     2461 gcattgcctt acagcatggt ggtatttcat gggcattagt aggttatatg tacgtgtaca
     2521 gtatgtattt gttttttgat tttgctggct atagcttatt tgcagtcggt acaagctaca
     2581 tgatgggata tcagacacca atcaatttca ataaaccttt tttatcatgg aatatcaaag
     2641 aattctggaa tcgttggcat atgacgctgt ccttttggtt ccgtgattat gtatatatgc
     2701 gattgatgtt cttcatgatg aagaaaaaat tttttaaaag caagatcgta atgtcaaata
     2761 tcggttattt tgctttgttt cttctaatgg gtatatggca tggattgacg tggtactaca
     2821 tcgtatatgg actgtatcat gcttgtctga tctgtctgac agatgcatgg atccgattta
     2881 agaagaagca taaagagcag ttgccttcta acaaatttac gcatggctta gccgtatttt
     2941 tgacatttca agcagtatgt tttagcttcc tgattttttc aggattttta gataaacttt
     3001 tttttagcta acgaatagtg acatattaat ttatataaca aaggagaaaa ggaaaatgga
     3061 agaacaaatt ttatcaatct tagaagaaat aacaggaaca gatgaagtaa gaaaaaatcg
     3121 tgatattgat ctatttgatg aagggttgct tgattcttta gcatctgttc aattattagt
     3181 cgagctagac ggagagctgg atatccaagt ccctgtatct gaattcgagc gagaggattg
     3241 gtgcacaccg aataaaatca ttcaacaagc aactgcattg aagggttgag tatgaaaaag
     3301 aaactttttg ctattttcgg cccggttgtt ttagctgttg gcttactgtt tgtgtttttt
     3361 acctcctcct atcaagtaga tatctctaat ccagaaacga ttagacgtgc ctcttcttcc
     3421 atgtcagtga acgtgttgaa aggagatgcg ataaaaaatt atgcattatc tgaaaaacaa
     3481 tatattcctt tttttggctc aagtgaatta agcagaatca gtccatttca tccgtctgtt
     3541 ctggcagaaa aataccagcg aaactatcgt ccatttttgc tgggagcacc cgggacacaa
     3601 tcgttaagcc aatatatgat gatgcgttct gcaggagatg ctatgaaaaa caagaaagtc
     3661 gtctttatta tttctcctca gtggttcgtt aaaaatggtg taaaaactga ttactttaat
     3721 acgtattatt ctgaacttca aacatacgac tggttgtttt caatgaagaa agtaacgcct
     3781 gctgatcgct atttagctag aagattatta actttctcaa aagtaaaaga gaatgatacg
     3841 ttgacggcga ttctgcaaac gatcaaaaaa gggaagctac ctttacctga atctttgaat
     3901 cagctgcgca gccaatggaa catgctgaag agagaggatg aagtattcag caatattggg
     3961 ttgaaagatc gacagcaaaa aatcgatcat gagtcaaaac gattaccaaa acaataccaa
     4021 gaaacagaat tatccatttt agcaaatcaa attggagaac gagaaacgac gaataatcca
     4081 ttcgggttga aaaacgattt ttatacgcat cgtatccgag cccacgagcc agagctcaaa
     4141 cagtcacaaa aaaattggga ttatcgtttt tcgccagaat tttctgattt tcagctggtc
     4201 ttggatcaat tagcaaaaaa ccataacgaa gtattgttca tcatcccacc ggttaacgag
     4261 aaatggagcg attataccgg actatctcaa gaaatgttac aaggatttgc caaaaagatc
     4321 aaatttcaat taaatagcca aggattcaat cgaatagctg attttgtcaa tcaagcgggt
     4381 accaactatt ttatggaaga cacgatccat ctaggctgga aaggctggtt ggctgcagat
     4441 caacagatca gaccgttttt agaagaaaat catattactg ctagtaagta tcatcttgac
     4501 gatgcttttt ttagcaaatc ttggcaacat caaataccag ataaactaca attaaaataa
     4561 agaaaacagg cgagtgttgc tcgcttgttt tctttatttt tgaatattct gaataaaaga
     4621 taaaaatacc aagtttgcct gaacaaaaat cataagcaat ggtataatca caatatgaat
     4681 atagaaatgg gggaaaacat ttggatacaa aagaagaaca gctacttatt gatttaactt
     4741 ctgcaaaagg agtacctgga aatgaagaag aggtaagaga agtattcaga gaatatgcca
     4801 aaccattcgc tgatgatatt ttttatgacg gtttaggcag tgtgattgcc aaacatggtg
     4861 ctggcggagg ccccaaggtc tttatttctg gacatatgga cgaagttggt tttatggtaa
     4921 caaaaataac cgaaaaagga ttcttggaat tccaaactct tggaggttgg tgggggcaag
     4981 tcatgcttgc tcagcaagtt gaaatcaaaa cacgtgaagg gaaaatcgtc cacggtgtga
     5041 ttggttccaa accaccacat gttttaacac ctcaagtaag aaataaacct tatgaaataa
     5101 aggatatgtt cattgatatt ggcgcatcaa gccaagaaga agccaaagag tggggcatac
     5161 gtcctggaga tatggtcact ccatatattg aatacaaacg aatgaatggc agtaaatatt
     5221 tactggcaaa agcatgggat aatcgtatcg ggacagctgt ttctctgcga gtattagaga
     5281 atctatctaa agaagcacac ccaaatgttt tattcgcagg aagcgatgtg caagaagaag
     5341 taggcttgcg aggtgctcga acaagtacac accttgtaaa tcccgatatt gcttttgctt
     5401 tagatactgg tacagcagga gacactccag gaatgacacc aaaagaagca gattcgattt
     5461 taggaaaagg cccacagatt ttgatttttg atgcatctat gatcccgcat aaaaaattat
     5521 tgaattttgt tattgatgta gcagaggaaa tggatattcc attccaatat acagtgattt
     5581 ctggcggagg aactgatgca ggacaaatgc atttgacaag agatggtgtt ccgtctgtag
     5641 ccattaccgt tcctgttcgt tatctgcatt cccatacatc agtgattcat gaagatgatt
     5701 atttgaatac agtgaaacta gtcacagaat tagtgaaacg gcttgatagt gaaacagttg
     5761 aaaaaattcg gagttatgaa taatgaataa tagaatacct atttctgaat atcggaaaaa
     5821 agcacgagtt tctttggaaa atcaatgggg aatcaatact tggcttgttt tcttatctat
     5881 aatatttacg agcgtcatcc agaacttttt tgacggtttc agtccttttc cggaagaaag
     5941 catgcagcag cacttattga gttttctagt cgataattta cttttattcg cattcaacta
     6001 tgcattgttt tacattgcgc tagtagttgt acggggtggc agaccaaaaa gtaacctttt
     6061 attttcagtt ttccaaagaa agtattttgg ttcgatactt ggcatcaatt tgatcaattc
     6121 agtcatcaac tggctactca acagtttaat ctttctgccc atgtttttaa tcagcggagt
     6181 gagtacttat atgcagctgc ttttcagttc aaatgcggta cctacgggtg tcatgccaga
     6241 agattttctt gatatcagct ttgttttatc gatgataggg atgatttttg tttcagcact
     6301 catcatgagt attgttggcg gtttattcca atttgctgca tggacaaagt tcgactatcc
     6361 tgatttgtcg gttattcaat gtttgaaata tggctggttt ttattgaaag atagaattgg
     6421 tacgtatatt ttgttgcagc tgtcttttat cggctggtat ttgctaggag caatcgcttt
     6481 gttcatcggt cttttgtggg tcattgttta tgtgaatgtg tcagttgcag aattttatga
     6541 acaagcacgg attgaaaaag gaggtccaga cgcctatttt acttagcatg taagcagttt
     6601 attcatcaaa aaaataactt ttgtggtagg ataaatcttt gagggggaat gtacgatgaa
     6661 attaactgtt ttaggatgct taggagcgta tccttataaa aatcaaggga caacaggtta
     6721 tttgcttcaa tctgacgggt tcaatttgtt gatcgatgca ggtagtgcta cgctgatcaa
     6781 attacaagaa caccttgatc cacttgatct agatgctgtg atccttagcc attatcatca
     6841 tgatcatatt gctgaccttg gtgtattaca gtattactgg caacttcatc ctaagagaaa
     6901 tccggagaca ttgctttcaa tttatggaca cacaaaagat tccattcact ttgaagaatt
     6961 gacgttggaa aatgtctcaa aaggacatcc ttattttgaa gctgaagaat taaaaattgg
     7021 tccttttctg attactttta tggaaacaat ccatccagta gtatgttacg ccatgcgtat
     7081 cgtagaagaa acaacgggga aagtattcgt atttacaggt gattcaggtt atttggaaag
     7141 tttcgctgat tttgcgaaag atgctgattt gtttctagca gatacctatc tttttgaagg
     7201 aaatgaacat catcatgccc atttcacttc aaaagaagct ggagaacttg cgaaatcagc
     7261 taacgtaaag aaacttgtac ttacacacct acctcagttt ggtgatttag aacaattgaa
     7321 aaatgaagcg caaaaggcag caggcaaagg gattgatata gaacttgctg cagttgacaa
     7381 agtgtttaac atttaggagg aagaacatga tttttgtacc aaatgaaaac cacgacccaa
     7441 gaatcaattt agcaattgaa caatatttgc tgcaagaaat gcctttagat gaacctattt
     7501 tgctctttta tatcaatgaa ccttcaatca tcatcggcag aaaccaaaat acgcttgaag
     7561 aaatcaatcg agattatgtg gaagaaaatg gcattcatgt tgtccgtcgc ttaagtggag
     7621 gcggagctgt ctaccatgat tttggaaacc tgaactttag ttttatcatg cctgatgatg
     7681 gggattcatt ccgtgacttt gcgaaagtaa cacagccaat catccaagca cttcatgaat
     7741 tgggtgttgc tggggcggaa ttgaaaggcc gaaatgatct agtcattgat ggcatgaaat
     7801 tttctggcaa cgcgatgtat gctaccaacg gtcggatgtt tgctcatgga acaattatgt
     7861 tcgactctga tatcaatgaa gtagtcaatg cattaaaagt aaaaaaagac aaaatcgaat
     7921 caaaaggaat caaatcgatc cgttctcgtg taacgaatat caaaccattt cttccagaag
     7981 aaaagcaaaa aatgacaaca gaagaatttc gtcaagaaat cttgttgaaa attttcggcg
     8041 tagagtcagt agaagaagta aaaacgtacg aattgactga tgaagattgg aaaaaaatca
     8101 atcgaatctc tgaggaatat tatcgaaatt gggattggaa ttacgggaag tctccagatt
     8161 tcaatttctc tcgccaaaaa cgtttttcta tcggttcgat tgaagtccat ttgaatgtct
     8221 cagaaggaat gatcaaagaa gcaaaaatct ttggcgattt cttcggacta ggagatatct
     8281 ctgacgtgga aaaaaaattg gttggacaga aatatgataa agcatctcta tcaaaaatag
     8341 tcagccaaat tgatgtgaag aaatattttg gtgcgataga accagaagaa ttgcttacct
     8401 tactttattg aaaataaaaa atagcggaat ccacatatat ggattccgct attttttata
     8461 caggattcgg gtaatttcga gagagttgaa cagtgatccg atcaatgaaa gtatcttttt
     8521 cctgcgttag tgttcctgtg taacgatccg ctacgtcttt catcatcatt aagtcaaaac
     8581 gttcctgatc atctaattct tctgtgatgc ctcgactaaa taggtttgta tcgtaatgtg
     8641 cactatgttc gcaacaaatg acgaaatgtt cctcttgttc gtgtatcgac aaataaattt
     8701 ttcgatacag tggattttcg atggcatgag tttctctaaa ggaatgctct aaagcgtgga
     8761 tcaaaagttg agccagatcg tcatcgttga taggaagttc ttcgggaaga tctgcttgga
     8821 aggttatctg aatattccgt ttggcagcta acttctggta acgcgctaag ataagatttg
     8881 tcacctggtg gttgcaaaaa gaacacagca tttcgaaatt ttttgcttct tctaatatct
     8941 tttccacata gtttttagct tttgtttggt ccgtatgcat taaatcttgc agattctcca
     9001 tctcttggac aaattccagg cgaagtgtat ttacttcttg gatatgctgt cgaagctgct
     9061 cataatgttg ctccaacaaa tcatttttga tttttaagaa atctacttgt ctccgatctt
     9121 tgtcggtatt taagatgtga tcgattagat tgtagctgat aataacgatt agaatgataa
     9181 tatataaaat cgtagaccaa ttgatcgttg tttgatagat acctaatgcc gtttggatgt
     9241 aaatcaagca atggataatg gctaccaaga cgatccaagg tgtacaagct acataaaaac
     9301 ggttattctt atatgcttcg ccaatgcatg caagtgaagt gactaaagtg cttataacac
     9361 tagcaatatt agcaatcggc attaattcag gaagttctgc aggagtaaaa gttgcataaa
     9421 gcagaacgcc gataaaaatc aaaagttgga acgtacaaaa aattccgtac cattttttat
     9481 agtaaaacat tcggctccaa tagtagcaga tgatgaaagc tgacgttaag ataataaata
     9541 aatgagataa aaaagaatga acgaatggtt caaaaagaat accacttaaa atatcttctg
     9601 atacagcttc aaatcctaat gccaaagcaa agcaacttaa gagaataagt gagtattcaa
     9661 gacgtttttg tttatacatc aagtagatcg tatagaaaaa ggtccctata gcgataaaaa
     9721 cagcgattat catcgtcaat acttgcggca ctgattgtga ataaataaaa ctaatcaatg
     9781 gtccagttgt tcctagataa actttcggtg gcagtccagc atagttctga taaggagatg
     9841 agactgtaag ggtcaatttt tttccgatat aatttgctgg taaatctatc acagccaatg
     9901 agagtccggg attgctttct tcctgataat ctttccgttg gtataaaatc ttttcgccta
     9961 gatatacagt aatccattga tggtttcctt tgattagtaa atttgcttcg tccaacggtt
    10021 tttcaagtgt cgtttccata atgaatgttt cgtctttatc cacggtagga atcgtacgag
    10081 tgtacttgct gtcaaactta aaatcaggat catcttttgt atagaaaacc cacgaatctt
    10141 ttatttcttt catttgattg ctgtccgcag gagaaaaaat aaaataaaag aaataagcaa
    10201 ttcctgttaa aa
//
LOCUS       DANKHL010000101        10166 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000101 DANKHL010000000
VERSION     DANKHL010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.105"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(133..651)
                     /locus_tag="PW101_001774"
     CDS             complement(133..651)
                     /locus_tag="PW101_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL2514173.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(653..1270)
                     /locus_tag="PW101_001775"
     CDS             complement(653..1270)
                     /locus_tag="PW101_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDL2514174.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1267..2121)
                     /locus_tag="PW101_001776"
     CDS             complement(1267..2121)
                     /locus_tag="PW101_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2514175.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(2118..3029)
                     /locus_tag="PW101_001777"
     CDS             complement(2118..3029)
                     /locus_tag="PW101_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2514176.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3070..4404)
                     /locus_tag="PW101_001778"
     CDS             complement(3070..4404)
                     /locus_tag="PW101_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2514177.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4612..5442
                     /locus_tag="PW101_001779"
     CDS             4612..5442
                     /locus_tag="PW101_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDL2514178.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5686..6004)
                     /locus_tag="PW101_001780"
                     /pseudo
     CDS             complement(5686..6004)
                     /locus_tag="PW101_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6126..6320)
                     /locus_tag="PW101_001781"
     CDS             complement(6126..6320)
                     /locus_tag="PW101_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514179.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6825..7010)
                     /locus_tag="PW101_001782"
     CDS             complement(6825..7010)
                     /locus_tag="PW101_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514180.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(7860..8201)
                     /locus_tag="PW101_001783"
     CDS             complement(7860..8201)
                     /locus_tag="PW101_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2514181.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(8201..8431)
                     /locus_tag="PW101_001784"
     CDS             complement(8201..8431)
                     /locus_tag="PW101_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2514182.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            8718..9056
                     /locus_tag="PW101_001785"
     CDS             8718..9056
                     /locus_tag="PW101_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514183.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(9261..9500)
                     /locus_tag="PW101_001786"
     CDS             complement(9261..9500)
                     /locus_tag="PW101_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDL2514184.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(9476..9823)
                     /locus_tag="PW101_001787"
     CDS             complement(9476..9823)
                     /locus_tag="PW101_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDL2514185.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT         3299 a         1979 c         1694 g         3194 t
ORIGIN      
        1 attttataac gttctcattt aacaaaactt aagttattaa gacaaatgaa taaacaacta
       61 cgctttcctc gaaacaaaaa aataaagcac aaatgcgcat ttacgcactc atgctttatt
      121 cgtacaatta attcagataa cggcagcgca taggatgcca ataacacttc ctaaaatagc
      181 tcctcctaaa atgtcagtag gataatgtaa aagtaaaaaa atcctcgaat aagcgatcaa
      241 acaagcaaat aaaaaggcta atattcctgc aactggactt aattcgaaca acgcagctac
      301 tgataaaaag gctagcgctg tatgtcctga aggaaaagaa gaagtcttcg gtttacttgt
      361 gtaaaggaca agccgacgat ttttttcata aggtcttttc cgtcttgtcg tttttttgat
      421 aaaaagcaaa ccaacaaaaa aatagatcaa actgccgata agaatggcta gaccatattc
      481 tctagtatca ggtatcaaga ctaaaacagc agcaaggagc aaccaaataa aaccaccatt
      541 agagatatta ctgataaatt tcaaaaaatt gatttttcga ctatccgttt tccgttgctt
      601 ggcgaacttg ttgataatac tatcatcgat tttagcaatc aaacgcaaca tgttattcct
      661 cctttttaag aaccttttct tgcgtacgat ggataaaaag caggacttga aaataaaaca
      721 agaaactgaa acctaataaa gcaaaataga ttggtcctgc aaaccagaat gcgacaagaa
      781 ggatcaacgt gcctgccaaa tagaaaagcg cgcttttgaa atcattaaaa aacaagaaaa
      841 atgcagtaat ccaagaagct tctttttgtt ccagttgaag caagattttg taactaaaga
      901 gaagcgcgct taatagaagc aaaaacaacg tagtatacat caatgtatag ccaattcccc
      961 caccccaata gcggaaaatc tgataatcga tcaatagaag aagaaaataa acacttgcta
     1021 aactatcttg ctttccataa agagagaagt tctcacgaaa ctttttccag aatagtgaac
     1081 cgagcttttt ctcttcattt tttttgtaat gataaatcat atacagaact gttcgacttg
     1141 cagttcctaa tgtgaaaaca ggcaagacgc acaggaacca tagtagctgt aaatggataa
     1201 gacgtggaat ccattcaatg atttctaaga aataatcatt ggctttttta ccaatcgatc
     1261 gattcatcat ccttttgaac ctcctgtcaa atccatccct tcaatgaaat aacgttgtgc
     1321 aaaaaagaac agcaaaatcg tcgggatcaa gacaagtgtt gctcctgcca tcagataatt
     1381 ccattgtgtc gttgcttggt tctgaaaaac ttgcaagcca atctgtaacg tgtatttttc
     1441 ttgatcttgg atatataaca gaggacctaa gaaatcattc caagctgcgt tgaaggaatt
     1501 gatcgcaatg gtaatcaagg caggtttcgt caatggaagc atcaaatgac tccaaatata
     1561 caaatgattc gctccgtcga tttcagcagc ttcgatcaat tcattattga tcgataagta
     1621 aaattgacgc atcagaaata tattgaacgc attgccgaag aacgatggaa cgatcagggg
     1681 caaataagta cctacccagc cgatcttgga gaataataca tattgaggga tcatcgttac
     1741 aaaccccggg atcatcatcg tagaaagaac caaagcaaac atcagctttt tcccaggaaa
     1801 atcaagcttc gcaaatccat aagcaataaa agaatttgaa aggacattac cgatcactac
     1861 tagcacagtg atgaataatg tatttcttgc atagcgccaa aaaggaaagg cggcaatcgt
     1921 atccagatag ttttcccata cgattttttc tgggaaaaag gtcggaggat aacgaacgat
     1981 ttcttccatt ggtttcaatg aagtaaacac catccaaagc aatggaatga tcaaaatgat
     2041 tcctattact gtcatgagga cataacttag tatttttttt gtttgccgat tcacactggt
     2101 ctttttgaaa taatccatca tttgccgtca cctccagaat aatagaccca cttaggtgca
     2161 agcttcaaat tgacaaacgt cagaaccaac acgatcagaa acaggatcat tgacatagcc
     2221 atcgcataac ccatatcaaa agctacgaac gccttgttcc acatatgcag attataaaaa
     2281 agcaaagaat ttgcaggtcc gcctgttcca tttttcgtca tgacataagc ttcttgaaaa
     2341 atttggaaag aaccgatcgt agaggtgatc acatcgtaaa aaataatagg agtgattaat
     2401 ggcaaagtaa tacttttgaa ctttccccaa acgcctgctc catcgatttc tgctgattca
     2461 tataaagatc taggaacatt ttgtaatgta gctaagtaaa gtagcattgc tcctccagaa
     2521 ctccatattt tcatcaaaat caacgcaggt ttcgtccatt gtggatcaaa cagccaagct
     2581 ggtccaggga tatggaacca gcccagaaat gtgttgatca atcctgcaga tggagaaagc
     2641 agctgcatcc ataagatgta gactgctact cctgataaga ctgccggtaa atagaaagct
     2701 gtacggaata cactgattcc tttgattttt tgattgagta ctgtggctaa aaacacactg
     2761 aaaatcgtcg tcagcggtac attgaagatg acgaaataaa cggtgttgta taacgatttc
     2821 caaaataaat catcgtgaac gaacattctt ttgaaatttg ctagaccaat gaaattcatc
     2881 ttggatgtca aattataatc ggtgaaacta ccaataactg aaaaaagcat aggccctagc
     2941 ataaatacca agaacccgat aataaaaggc gagataaata aataggccca tcttgcttct
     3001 tttttctttc ggatagataa acgcttcaaa ttttcttgcc tcctctgtac ctagacagtc
     3061 aaaaatggat tattgatttt gcttgactaa gttttctact gctttttgtg catctgccaa
     3121 tccttctttt ggtgattttg ttcctaaaag aatctggtca atctgttcat tcactaatgt
     3181 cgtgtaatcc ggagcatcta aaggaactgg tgtgatcacc gtatttttaa agttttcgtc
     3241 cgccatttga tagatcattt ttcctttatc cgttaggttg tcattattca ctaaattctc
     3301 attggctgtc ttgctcgcca tgatatcaaa acttttttcg ccgaattttt cttgtacttc
     3361 tggagaagaa agatatttga tgaattcata agaggcctca ggatcttttg ctccgtatgg
     3421 tacttccaag acgaatccgc caccccacga ccaatggcct gaaccttctt ctttttctgg
     3481 gagctgcatc acgccaaaat caaaatcatc cggtgcattt tccatcaaac tagagtaata
     3541 attgatattt tgggcacgca tggctactaa tccggaaata aatggatcag atacaccaga
     3601 cccaaattcg gcttctaatt tattgatcgt atttttccca taataatctt gccagtcttg
     3661 gatccactcc aaggcttcca ctttattttt agtatcaatc ttgacttttt catctttgtc
     3721 aaaccaacta gtcccgttat ctgcgtttag cgcccataca tcagccccta aattccataa
     3781 tggataaaaa ccgatgcgtg taaattcttt tccttctttt ttatctagtt tacgtgctgt
     3841 ttcttctaat tcatcccaag ttgtcggtag atcttgttca ctgattccag cttctttaaa
     3901 taacttttta ttataaaaaa tgacttgtgt atctgtattg aatgggatcg cataaggatc
     3961 accttcatat tctactgtat cccaaagctg cggataaaaa tccttagaag tgtctttttc
     4021 tacatatttg gagagattag ttgcttgctg tgcttctgct ctttgggcaa cggaatttat
     4081 gtcctgtaca ataacatcgg gtgcatttcc agctgttatt gcagataaag atttagtcca
     4141 gatgtcaccc catggctgat acttataatt tacatggatc tcgttttgtg attgattgaa
     4201 atcctcaatg atttcttcga tagcttcttt tcgtgcaccg gatccccaaa aggaccagaa
     4261 atcaatattt tttcttccat cacttgtttt ttcagaagaa tttgctgcac ttgaactggc
     4321 atcgcttgca ttacttgatc cgcatcctcc taataaaaac aatgctgaac ttgccagcaa
     4381 ccctgtaaac aataattttt tcatatttta cctccaaatt ttaatgtaac cgatgccggt
     4441 tttaatataa cgatatcatt ttgttctgaa aataacaatt tataaggtat gattctaaaa
     4501 aaattattaa tgataccaat actttatata attcgcgagc gctttctttg aattttttaa
     4561 tctttcgtag cagaatgaga atgtattaaa aatagattag gaggaatttt gatgtttaga
     4621 caccagaaag aattgcagtt tgaagcaaaa ccagatcgtc cgaatccatt aatcgcgaaa
     4681 tatctgcagg agctgatcgg cggccaatat ggcgagatga gtgtagctat gcagtacctg
     4741 ttccaaggct ggagctgccg aggagaagaa aaatataaag atatgctgat ggacattgca
     4801 acagaagaaa tcggccacgt ggaaatgttg gcgacaatgg ttgctcgttt attagagggt
     4861 gcgcctgttg aagatcaaga agcagcgatg caaaaagatc caacaatcgg tgccattatc
     4921 tctggaatga atccacagca cgcgatcgta gctggcttag gacctcgtcc tgctgacagc
     4981 gtgggcaatc catggagcgg tgcctatatc attgcgagtg gcaacttgtt agcagacttt
     5041 agagctaatt taaatgccga atcacaagga agacttcaag tagcacgtat ttacgaaatg
     5101 atcgatgatc gtggcgtaaa agatatgctg tctgttctcc ttgcaagaga tagctatcat
     5161 caaaatctat gggaatgtgc agtgaaagaa ttagaagaaa aagaaggttc aatcttagtc
     5221 ccaagtactt tcccgcgtga caaagaacga aaagacattg cttatgactt ctataacttc
     5281 tcagagggag accaaagtag tgaaggttct tgggctagtg gcaaagcact ggatggcgaa
     5341 ggcgaatatc aatggttgaa agaacctaag atcgaaggcg ctgcaccaaa attagaacca
     5401 gtaacaccaa aaatgtatgg gacacctcca ttcaagaaat aaatatctac ccgtagaaac
     5461 acccgttttt tgttccacac gctttgtgag caaaactgac taaagtcatt aatcataacc
     5521 acccttcaaa agggtggttt tctctagagc tataagctca aatcaccggc cagcgcctaa
     5581 agacgctggc tttcatattt tatgttcaag ccatattgcc tttgccagtt ttgcccacct
     5641 cttaaaaggt ttatgtacta ctccttagcc cttaaaagga tcgtcttact ctcgtacact
     5701 cagcttatct aacgcactat catgtttttc ttgttcttga atgtactttt tgatggtcgc
     5761 ttcattttgt cctacttttg tcacatagta tccttccacc caaaagtgtc gatacccaaa
     5821 tttatatttt agatttgcac aatctcttga tgatctcctg aatattggct cgatattgat
     5881 tataaattat ttttcttcta tactttggtg tgaatacaat gtggtattta cacatctact
     5941 ttgtacgggc taaactattt ccctttattg ctccacccgc atagcaagtg gttttgtatt
     6001 tcaatcgctc ggcgcgctca attaggtcta aagacaataa atctaaaaac ccgctgcttc
     6061 actgacagaa acaacgggtt ttcagaaaaa aatgaaatga aaaagatctc ttacggcaat
     6121 ctattttatt tttttgattt tttataaatg gaaaatgctt caagaagtcc aagaaataaa
     6181 gccgtataaa aacaagaacc tatgaaatct ttatttatta gatattcaac agaaatcccg
     6241 ataacactag ccaacaatat atttatcaga agtgtaaaat aaggatgctt tttatagaat
     6301 agaactagtg ttgttttcat agactctcct ttcttcatat ttccaattaa tcacaaattc
     6361 acggcattac tagaaaaatt aaaggggcgc agaagtggaa aaatactctt cgactttccc
     6421 tacaaaccag gccatcttgt gtttaataac tataccaaat ctaaggtacc tctaaatcta
     6481 aattccattt gtggcggtga cctccaattt ttaactcttc tatctcccat ccagtataca
     6541 taaccttgat acttcgacca cttatatatc gaacacacaa atacctatcc attgcaggag
     6601 tactacctaa cttagttgct acttgcatct aaataacagc tgaaaagata ggacttactc
     6661 ttagtaaagt atctgaacta ttaattttta aactatcatt tttatcctca actacatgca
     6721 catctgttgc aacttcactt tctgtctcat ccagataaat gattaaggaa tcgggaattg
     6781 caatatcttc agctgacgct ggaatagttg agctaaatta gtcatcatat ctttattata
     6841 aaataaccaa cagcaaaaaa tacaatgaaa aacaaacttg gaactaatgt atcattaata
     6901 aatgttgaaa aattcagcaa ctcattactg tatgatttta gcagcccaac caacatatac
     6961 cctataaaag ctaataagat ggttaggaat cctttagaat ttttactcat tcatagacac
     7021 ttccttaaat aacttttatt atagaaggta ttgtaaataa taatattgac aaaaagtgac
     7081 tcaaaataaa aaaaacctta cattatactg gaatgaactg tattgatagt gtacctaaaa
     7141 gctctactga cataagatcg tgtaatcggt aaagtaattt tattaattat acgggatgta
     7201 atcaattaga gaataaacta atcataaaag gtacttttac attcataatt gtcgctttga
     7261 gattctactt tggttcacta tctgacccaa ttgcacattt tcgatcatta caagtattgg
     7321 tgtcgttaga acaactaaat aaaaactagc ggtagatttt tcagaatcta tgctaatttc
     7381 tgtatggatg ttccagtgat ggcaaatttc tcttttcatt gtttttcttg tcgcatgata
     7441 tgcctttttg tgttattcag ttaaactata gaaattcttt cctagacaga tcttgatccc
     7501 aaattttacg tttttgagca tcgatatagt ttaaaatgac attactcata tctaaagagt
     7561 ttagattttt taggactaaa actgtcagtt gttctttttc ttctttctgt taaagtattg
     7621 tcgcgacttt tcgtatacgt taagatatgt cgtgaaaaaa caagttttgt gacacaaaaa
     7681 aacgaatgac ttgaggaaat cacctcaatc attcgtttaa aatgctatat attctttagt
     7741 ttaagatacg gaaattaatg ccatcgacca cactagtaga atgaacgaca tttacttggc
     7801 ctttttttaa tttcgttatg tttagcatat aaaaatatta tagagtttac ttacaatgct
     7861 taaaacatat actgtataat ctgatcaatt ttagccatgt cttgcaatgg taagttttct
     7921 atttttttga gttttcgttc cttaaaatca agagatttta attgtgtaat aattacttgt
     7981 ccttttatat ctagctcttc aggtaatgag taacgtgtcg gtaaattctt tatagtggaa
     8041 gtaattggac atatgaccga aaacattgtt ttacggttaa attcataacg agagacgact
     8101 agtcctggtc gtcttttttg aatttctttt cctacggagg gatcaaaatc aatccataca
     8161 atatcacctt ttttaggaat ataaattctt tcttctgaca ctataaaatt tctctccctt
     8221 caggagttaa atcttcccat tcgtctttct cataatactc ggcctcttcc ccaccactaa
     8281 atggatcttc aatttttgga actaacgtaa ttgttccatc atcagaatac acaacgatat
     8341 attcttggtt ttctgaaggt ttctttccgt tatcagaagg taaagttacc actacagaac
     8401 ttccttgtaa tctagatttt gtaacaagca ttatcatcac tcccttaata aaagtatact
     8461 atataacact aagtaacgca aaattcccct ttgtaaacta tctagaaaat tgttttgttt
     8521 aaagtatatc ttattggata taaatagaaa taaattgtag ccagttcatt ttttctttaa
     8581 ctaaaaaaat aatccaagaa acattgaacc tatggatcca aaagctaccc aaatactata
     8641 gctaaccccc cgctaccttc aattttgcag ataaaacgct ttgggagaaa atttcaaaga
     8701 caacaggcca tcctatcatg cccgattata aacaaaaagc tggctattcc ttttgctctg
     8761 acaaatttca attgggaaag ttcacgaaac tagcaggtga agaagtttca actgttcgga
     8821 tcaaagaatg cccgcttcaa atagaaacat tggttcaacg aatttcaact agacaagact
     8881 ttgctattgt agaacgtgag atcgtagcta tttttgtaga agaagggatt ttacatgatc
     8941 caactcatat caatgtggat aaatggcaac ctcttatcta taaattcaga gaatatacaa
     9001 ctgccaacca gtcgttaact ttgaatttta gattccaaga attcaaagag tcataatgat
     9061 aaacatcgtt aggtataaat aaaaaagact cgcagcacga gtaaaaaaca aatagaagct
     9121 atacattaga aaggtcgact gtgaccctat agatgcagta tatgcagctt atgagcattt
     9181 cttctgttct ctttgttttt gggatctgtg ctgaagtctt tttcgttttt tgccgctctt
     9241 tctttgaaaa atcgattatg ttataccttc tccattacta attcaggcat ctgctgagta
     9301 atgatttttc gcgcaaaaga aatatctgtt acccaaggtt cgatttgttc ttcagtcaag
     9361 acaagcggca ttcggtcatg gattggtgcg actgtgcggt taggttcagt agtcattatg
     9421 attgattctg ccattttttc atcctgatgg ctacgataaa aaccgccaat atacactatt
     9481 ttttccttat ttgaaaacat aaatttgtct ttttcttcat tccactcatt gggaaaacac
     9541 aacgattttg caaaaatggt tgatgaaagg tctttttttc ttctactgtc tctgcacggg
     9601 cattaatcat caattgtcct tttttaaatc cggtaaatcc ccacttcatg atttccggag
     9661 tgaggatgtt ttcttcctct tttcttaacg tcacaacaag atttgacgga aaaacttctc
     9721 cagctgctaa ttctgctgcc tgcttcggtt gtttcgtctg ggcttctcga taataattct
     9781 gaaggagctc tgactctaga tcaaaaaaat aacgaccaca catcctaatc cctcccaatc
     9841 tttttttcta tcatacactg caacaagaag ttcgcaccaa caaaacgctt tcaatttttg
     9901 tagaactaaa aaagttgtga caagtgacaa actctccagt gaatggtatt ctcctgccaa
     9961 ctcctcggtc acaagtcaca atattcaaca aacatgagaa gctgcttttt gaaattgttc
    10021 cttgtgactc ggagtgaaac ggcaggctta cgacctctgg gtaaacggtg ttcaaaagcc
    10081 agtttctcgg tattaagcca aatttatgca aaaatgtaga aagctatttt cacaaaattt
    10141 ttcttaatac tcgaaactaa gcgact
//
LOCUS       DANKHL010000102        10077 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000102 DANKHL010000000
VERSION     DANKHL010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.106"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(34..186)
                     /locus_tag="PW101_001788"
     CDS             complement(34..186)
                     /locus_tag="PW101_001788"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL2514186.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(377..1255)
                     /locus_tag="PW101_001789"
     CDS             complement(377..1255)
                     /locus_tag="PW101_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2514187.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1294..2412)
                     /locus_tag="PW101_001790"
     CDS             complement(1294..2412)
                     /locus_tag="PW101_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDL2514188.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2409..4508)
                     /locus_tag="PW101_001791"
     CDS             complement(2409..4508)
                     /locus_tag="PW101_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HDL2514189.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4559..5380)
                     /locus_tag="PW101_001792"
     CDS             complement(4559..5380)
                     /locus_tag="PW101_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2514190.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5377..6186)
                     /locus_tag="PW101_001793"
     CDS             complement(5377..6186)
                     /locus_tag="PW101_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2514191.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6207..6701)
                     /locus_tag="PW101_001794"
     CDS             complement(6207..6701)
                     /locus_tag="PW101_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2514192.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6713..7201)
                     /locus_tag="PW101_001795"
     CDS             complement(6713..7201)
                     /locus_tag="PW101_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2514193.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7278..9974)
                     /locus_tag="PW101_001796"
     CDS             complement(7278..9974)
                     /locus_tag="PW101_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2514194.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         3060 a         2266 c         1658 g         3093 t
ORIGIN      
        1 ttacgattta ttttatacgg ctgaaaaaat ctatcaccaa aaaaattcaa acattaagcc
       61 aaggattatg aagtcaacat ttcgaaaaat gaacatacat tggtaagcaa ctataactat
      121 taccacagac ataaagtaac acaatatatt actatttaag tgtacgccac cagtgaatat
      181 cctcaaggta tacagcgcac aacaaaaaga aaaaaggaaa cgcagaagca tttcctttca
      241 gtgatttaat gaaaacaaca cgatttgact tagagccttt ttatttgaat tcattttcgc
      301 tttatccaac ttcatttgat catttgatta ctcggttaaa tacttttgac atgagtatca
      361 atgaaacatt cattaattat ggttgcttat ttcttaccgc ttttctggcc aatgctaatc
      421 cgccgattat tccggcatta tcacctaatt cgggcgtaat aatgtagcta tcaattggtg
      481 gtatttcaac atatccattt ataagctcaa caaatttatc ctgaactttt ttcttcatgt
      541 gttcttgttt cattacacca ccgccaaaaa taattctatc tggcgaaaac atcagtgttg
      601 tattgtatgc acattgggca aggtaaaagg cttcaatctg ccaaaacgaa tcgtcagcca
      661 ataaattctg gccttttaca cccaaccgtt tttcaatcgc tggtcctgct gccatcccct
      721 ctaaacaatc ttggtggaag ggacagtttc ctgaaaaatt gtcctttgga tgtcgcttga
      781 tcaacatatg ccccatttcc ggatgactga acccttccac gaaagtccca ttgaacaagg
      841 caccacctcc gatacctgtt cccacagtga agtatgcaac acctgctaac cctttgccag
      901 aaccagcaac atattcacca tagcaagccg cattgacatc tgttgtccat tcaatcggaa
      961 tattgaaata ttgctttagc gttcctacaa aattgaaatt ttgccaagca agttttggtg
     1021 ttgatgtgat atgaccgaaa ctcgttgatt tgcattgaat atcaattgga ccaaagcagc
     1081 cgaccccgat gcctgccaat tgttccttaa aaggtttaaa aaaatcaatg acctgcttca
     1141 ttgtttcaac tggagttgtt gtgggaaaac tgactctttc caaaatcgtt aattcctcag
     1201 taccgactgc acagacaaat ttagtccctc cagcttcaat tgccccatat atcatcatag
     1261 gttcctccct tcgaatatgt gaaaaacact tcattaacgc ggcaagcgtt ctcgatcagt
     1321 gaaatgacgt ttagtatttc ctgaagcttc tgttgaatac cagtaagcga cactagcaac
     1381 atcgtcttgc cgctcaaata agcgaacatc atcgttgccg atttgttgta aagtgaccga
     1441 taaatcttcg gtaaaacaga tggggtcttg aagatgccaa cgataaagac catgtcgtgg
     1501 cattgcgtcg tcaccaaagg catgaacggc attcaatttt cccgtttcaa aacgatctct
     1561 tgtggcatct ctatttgatt ggtaaggata ccctaaaaag gctgttgaat agtttttcgc
     1621 atgtggacgt ccgaactcat cgtactcatg aaaggcccac gccccgccaa aataatcttc
     1681 tgaacctgtt gaggttacgg tcggatactc actatcccca tcaatgtaga atttaaactc
     1741 gccttcgccc caccaataac gttctaggca ggtaagcgca aggtaagtac ccacgtaata
     1801 gccttgaccc tttaatcgat caattaactt ataatcctcc tgtaatttgg taatgcgttc
     1861 ccgatgccaa tttgcgtgaa aatataggct attttcttcc aattcgtcca ccaaagcgta
     1921 atttatcgta taaaaaaatg aggacatatc cttcggatgt aaattctcga tcgtaatttt
     1981 agcggatgtt tggaaaggca tttcgaaata ggaattaaag ccccctgtag gattaaccac
     2041 tatcggcaac gagttcacct cgcaacgctc tccaaaccca ttacaaaaga aatcccctaa
     2101 aggactttca acagcagggc tatccgaccc atcccaataa attcttaaga ttaaatcacg
     2161 taaaacaaaa ctgccaaatt ctgttcgatc acgaatcgtc atccacatat gacgaattat
     2221 tcccgttcca gtaatatccg caatcacagt ttcttcacca gaagtcagcg gaattgcgcc
     2281 acgacctttg cggcttggtc ctagctcgct tggcaacatc gcagctttac ctttctcgcc
     2341 tgttggattt tcgggagtga ttgctcgact ctggattttt ttaaattggg taatattttt
     2401 tagtatactc atctttttct cctttgaatg tgaatcgttt ttcccttttc tacgtgaaga
     2461 atttttttcg tatcacttat tttcaatggc ttttctgaca aatttgaaat actcaactcg
     2521 gattgcgtac attcaatcga aatgaaggct tgaccgaact tcaaattctt ccaagaaaac
     2581 gttaaattat caacaggatc aatttgcaat actccttttt tgaatttcaa tccgaaatat
     2641 tgactgatca ttaggctgac aaaaagaccc gatgcccagc cgcatttgcc gcagccaaag
     2701 tctcgcacga cttctccttg ttgagcctct aagcgatacg gccaccacca ccacgaaccg
     2761 tctaaatctg ccaaacaaat tagttcttct aatcgggtta cgaaagcttg agggttgtgc
     2821 agctcgctca ttaaaacagt gataaagcca ggaaaagtgg cgccggattc taatccccaa
     2881 gtgatgcctt tgatttcctc gctataggtt ggattgaaag aactagcaga aaaggccatg
     2941 gtgttctgat ataaatgatc tttattatct aaaaattgat aaaaaggcat caacgtagtg
     3001 tctgattctt ctccatcatg catcaataaa ttaatttttc catccacaat cacatcactt
     3061 aaaaagatta acccttgctc aagaatcggt ttttgatagt gcttcactga ataggttgtt
     3121 ttctcctcat caccgattcc ctccaaaaat tgctcaccaa aaggaccatc gatagtcatt
     3181 tgtttacaaa ttgattcctt tagtttacag gcaatgtttt tgtatcgctt gctaagactc
     3241 agttgttcta gggcttcaaa aatttctgca agtccaatac aggccttcca aagacaaaga
     3301 ttgctgccgg tatgatactt tccaagtgca aaggcgtctg ataaccaaac agatggaaaa
     3361 agcattggtt cccctgaccg acgttgagct aatatgtcct caataattct tgaagcattc
     3421 agtagaacct gcggacaccg ctcaaagaat tttgtatccc cagtatgttc aaaataaaga
     3481 ctagccagta acgcactgga taacgaatta ctcagagaat gattgatacc tcctgtaaat
     3541 tttgaaccag gaaacttaac gccatattga tcaaaccata gaaccgtttt ttgacataac
     3601 tccggttcaa acatggtgaa tggcaatgat gcgtaataca tatccttatt ccaaattcga
     3661 ttagtagcag gatagctgcc ccaattgctg ccgacgacct gtccctgtcg attcataccg
     3721 aaactagaaa agctttgata aagtgcacga cgatagagat ggacaactct atcgtttggc
     3781 attgacagct gcccaaatag ctgagcaaaa tagttcaatg tttccttcag ccatttttcg
     3841 atatcagatt cctgaaaagt cattacttct tcaaaagcat ttgggtccac aaaagcaatg
     3901 gcgaagcttg ccacttcctg ttgtgcgatc gtgtccttat ttcctttttg agaaatcaac
     3961 acattttgct gatccgaata cttctcttga tacgaaggca catctttcac tgaaacttga
     4021 aggctttcgt gtgtttcatt cacaatgtaa acctgttgaa caagcatact taaacgctta
     4081 ttagccagta tgggagcaaa ggatactaca atgaccttaa acgtcgaata atgatgaata
     4141 tatctaggca aacagtcttg aatcacatcg acttcaatac gatgatccga ttctgacaaa
     4201 tggtagacaa tcccgtttac ttcaaccttt aaggataacg aattggtttg agagagctgt
     4261 ttattgaccc aaacacctgg tttttcctca gttagtaaat tttctcgata aaaggtcaac
     4321 gtttgaatcg tcccgcaaac atcaaggttt gctaatgtct tttgattact tacatcgaag
     4381 gtcaatccct ttacatcagg aatattccga tagtagctgt attcgtttga agtagtcaca
     4441 tttccgtcgt ttaactcagc attaaaaaat gggtgtttgg gtgaataaat tacttcatta
     4501 atagtcatct tttccctcct tgctaaaaaa actgcagcct agtagctgca gcttcctact
     4561 atgcgattcc aagtaatgac aataaaattc ccaagacgac cattccaaaa ataattttga
     4621 tgacgccgac ttttttatct agtaatttga aacagctcaa tgttaccaat aacggtacaa
     4681 ttcctttaaa aatactatcg aggatttctt ggacattcat tagttcttcg cccttcattg
     4741 aaaagctcaa aacggtattg aatttgacca tactgatcgt cattgcacct accatgatca
     4801 gacccaagat actagcagct tttgtcagaa tttcaattaa cccgttcgca tacagctttt
     4861 caatatattt agagcctaat gaatagccta aaacgccgcc atagtatcgc atcaagtaat
     4921 taggtatatt gaacataact aaaaagataa ttggtgccaa aatatttccg gcattggcta
     4981 agttgatcgc tagtcccgcg gtaacgacac gccaaactcc ccagaacaat gaatcgccaa
     5041 ttccagccaa tggtcccatc aaactcgttt tgatcgcgtt gatactttcc acgtcaaaac
     5101 cagctttttc agaattttcc ttctccatcg atgccgcaat ccccatgaca aaggacgcgc
     5161 aaggaatggt cgtcgaaaaa tactgtgtat gacgttccag cgccgcagct cgatcttctt
     5221 cacttttgta gaaatgatta atgaagggca tcatagaata ggcgaaacca ccgccgccca
     5281 tcgtcaccgg attcaccgaa gcatacatag tcatcgagcg ccaaaacatc ttccgaacca
     5341 attttttctc atctttagaa attccatcag ctaatttcat tgaaagaaat cctcctcttc
     5401 ttggtcaagc gttgcattta cagcagcatc gctttgaact gcatgaacag atatattttt
     5461 tagttgtaga tctctttggg ctataacaat tgcaataatc gttccaactg cggcaattgc
     5521 aatcaatggt aactcgagat acgccataac aacggcccca aagaaaaacc acacagcaat
     5581 atttttactc caaagcatcc gtaacagcat cgccatccca actgccggca gcagatttcc
     5641 agcgactgtt aatccgttca tgatgacctc tggaatatta tccaagacgc ttttgacaac
     5701 atctgctcca aataaaacgg caaagaatgg aataagagca taaagcgcat agtggacaaa
     5761 ccacataaag aagtgcagat tgatgattcc tttttgatca ccagattttg caaatttgaa
     5821 aaatttagca gccatcggtc cacatacaaa agaaaaaatc atattcatta actgtaatcc
     5881 taatactgct agtggaaaga caatgggaat aatcgcatcc acgtttcctc ccattttgat
     5941 tgcgaacgct gtagccagcc cagcagccaa agaaggctcc gccgtactag agattccgat
     6001 attgacgaca cccataaaga tggcttctag tgacgccccg acaatcaacc ccgtctgcaa
     6061 atcccccatc aacgctcctg ctaacggagc aacaacaatc ggacgactca gcatggacat
     6121 cccgaacatt tcatgtccag caactccaat aaaaacggcc aaagcgacta caaatgcatc
     6181 atacataatt tttctccttc ctttttctaa aaatcactca aaatagagat agctgaacgt
     6241 ttttccatcg ccggtgtagg ctgaaaaacg gtatctggat aaagttcaac taattctttt
     6301 aagtcttgaa tttctgacgt cgttaacatc acttcctttg ataaggtttt cttatcttcc
     6361 gatggcggtg tcattttccc agcattcccc acgttcatta attcgatttc cgttgggaaa
     6421 gctttcgcaa cttttaccgc atctttgaca ttagggacta aaaccatcaa gcgcatgtcc
     6481 ttcgcccgcg gatcttttaa caaatgaatc gcgtcatcaa ctgttttaat aatgactttg
     6541 acattcgatg gtgcagccat cttcaatgtc atcttttgaa tatcgttggt agccgcctca
     6601 tcgcttgcta ctactaaccc ggttaaattt agactctttg tccaagtcat cgctacttgt
     6661 ccatgaatta atcgttcatc aattcgcact gctttaatca tttttcttcc tcctaaaaaa
     6721 aatcttcctc ttcatttact acatgtacct catcaagaat ggtcatcttt aattcctgtt
     6781 ggcatttatt taccagttcc tgaatatcct cctttgttaa ttccttggtt gccaatacta
     6841 tttcgagcag aattggcaga ttaaagccag acaaaatagt tacagcaatc tctttctcca
     6901 aaaaggtttt ggtaatcttc tgattgacac ttccgccaaa taaatcggta aaaacaaaaa
     6961 tctgatcctg ctcgttcacc tggtttacaa atacattcaa ctcctgttca aaatcacact
     7021 catccacata agcattaatc accgtaatct cttgaacctt ccccgtcagt aattgaatac
     7081 tgctaagaaa gcctgaagct aagtgaccgt gagtcgctat tagaattttc ttcatatggt
     7141 taattccttt ctttctgttc acttttatat aagcaattcc tgtgccaact tttaaaaaca
     7201 ttggtttaat cacgttaatt cacattaaaa aaagtgtcta acctaaaaaa aacagattag
     7261 acactttttt agacacttta tttttgaata tacggtccta ataattcata gagcagcgaa
     7321 agctcataag cattgacttt aaaattatat ttcatctcca ttgattgaaa aatacttcta
     7381 gaaacataat aaaattcatc ttcatctggt ttcaaatcat aattcggttc ttcagatttt
     7441 tctgtccgag ccaagaataa acgttccatc atcaatgccg tatgcatata gagatttagt
     7501 tttacacggc catttaaagt gaaatgatag taattttcat atttagaaat gactgtttcc
     7561 acttcactca tcacgatcgt tgggttcaga aagttcaaac ggtccgcaat tccttccaac
     7621 gaaaagaatt ttactagctc ctgaatcaag cggttaaatt ctgccggctt catttcagga
     7681 ctcaaaatat gctccaaatt ggctgctcca tcaccgtcta agatgtcata aacattaata
     7741 ttaggaattg aaaaggaacg tggaagatcc gaagtcgtga tgaccaattt tgtcttgtca
     7801 aaataagttt catcattttg gtcgattaat tcacgtagtt ctctgtagtc cttcgtgaaa
     7861 acttcgatgg attcatccga taaatgatga cgaaaaacat ctttcaattt gtccgaaatc
     7921 cccaaacccg acatacagga aatgataatg ttgctacttt gagaaaagcc ttcaaagtat
     7981 tgaacattga ttgaatactt gtctgctgca tcctccgcaa tctgtttaaa tggcgcattc
     8041 atcgtcatct tcaagccaat atcaagtgca atggaagtcg tcaaattatt cagaagaagt
     8101 aactcccctt ttaaatcgtt cttgatctgt gtgtaaattt gatttaaaga gcccatatct
     8161 acgatcagca ctagactatc tgtattcact tgccgtttca cgaaattcct tgtcgattcg
     8221 acaatctctg acacattact ctctactggc atatccaacg cttcaaaaac ataattgccg
     8281 cataattgat tcactactgc ttgaatgctg ctagccgtac tatctccgtg agcaagcaac
     8341 aggcccttca gctcgatgga ttcatctaca taatcagata aagtcacgac aataagcagt
     8401 cttagaaagt tctcgtcctt ttctgagagc ggtggtaaat gctccaagaa ctgattacat
     8461 aagtaagtcg tccgaggtag atttgcagct aatgtacgat ttatcccggt taagtctgtc
     8521 ttatagcttg gggaaatgac ataggatctg ctaataattt ctgcaacagg tccactcgtc
     8581 gtcaatccgt acgtttttcc taaaacggat acccagacct ttctaaacat ctgaatcatc
     8641 aattttctac tcattgataa aaaagaatcc ttatcaatgt gatgcaccaa atgctgtact
     8701 tcattcttta tcgaagaaaa attttcattg aaattacctt catttatcag ctgagtaatt
     8761 ttttccaaaa tcgaatcata atcaaacggt ttgattcgat ccgtttttat cttttgattt
     8821 ttctttatca ataaactagc aatcggcaag ctttctactt cggattccag catatttaac
     8881 tgaatcaaga tctcattttt cccaatcgtt aatggttctt cttgtgttcg actatacaca
     8941 tccgcacaac tgattttaat gatattcttc agcttaccaa tattcccact aaagggatga
     9001 gataacatta aagacaaggc ttctcttgaa acaataatat ctttttgtaa aacatccgct
     9061 tcattttgaa aaaataattg aatcagctct aatcgttctc gaatggggcg atcttcaaaa
     9121 gttggcaaca taacactaat ttgaatacgc cgatcaaagg tgtctaataa ataattttca
     9181 cctttttccg tcgtggcgaa aataaaacga accttagcag aatgccaatt tacattttca
     9241 ccgactggac gatagtttcc agtatcaata aatagaaaga gcttttcttg attttctttc
     9301 gataggcgat ggacctcatc gagaaaaaga tagccgttgt tggccgttgc caataaacca
     9361 tcggtatcct tttcagcccc cgtaaaggcc cccttcttgt atccaaacag tgttgccgaa
     9421 agcagttcgg gattgtccgc ataatcggca cagtttaaga tagcaaatgg tgcatctttt
     9481 tcaataattt gctcatggat agcgtattca aaaatttttg tggctaaaaa actttttcca
     9541 acacccgtgg ctccatttat taatacactg agtccgttag gcggatattt gacagcggct
     9601 atacattttt gaatgacctc tctcagactt ccgtcatgac caactacaga atgaaaggct
     9661 tcgtttattt gctccgtatt atctacaacc gcatattcat ctaaaggctt ccacaaaaca
     9721 ggctttcccg gtaatttctc gaccttttta tcttccacta atctcgtcaa ataatggctg
     9781 gcattctgac gagagatacc tagattcttc gaaattttct cggcagtgac agaagacata
     9841 ttccctgtta gtcgtatcag ctcatcaaaa actcgatctt tagcgctctt tttcattttt
     9901 tcctccagtt taatgtgtct aaaagcataa taaactatcg gctgcaatat gtctattcta
     9961 tatttgtatt ccaattttta ctttgaaaca caaatagtcg aatatttgcc tcagatatct
    10021 gacaaaataa tttatctacc aatttagttt ttatctaata tgatttttat gtttttt
//
LOCUS       DANKHL010000103         9969 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000103 DANKHL010000000
VERSION     DANKHL010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.107"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="PW101_001797"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="PW101_001797"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDL2514195.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="PW101_001798"
     CDS             complement(1173..1868)
                     /locus_tag="PW101_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDL2514196.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="PW101_001799"
     CDS             complement(1881..2597)
                     /locus_tag="PW101_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDL2514197.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="PW101_001800"
     CDS             2927..3532
                     /locus_tag="PW101_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDL2514198.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="PW101_001801"
     CDS             3857..4180
                     /locus_tag="PW101_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDL2514199.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="PW101_001802"
     CDS             4362..5732
                     /locus_tag="PW101_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDL2514200.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="PW101_001803"
     CDS             complement(5862..7163)
                     /locus_tag="PW101_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL2514201.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7393..9351)
                     /locus_tag="PW101_001804"
     CDS             complement(7393..9351)
                     /locus_tag="PW101_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HDL2514202.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDKVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9528..9794)
                     /locus_tag="PW101_001805"
     CDS             complement(9528..9794)
                     /locus_tag="PW101_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2514203.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
BASE COUNT         2918 a         2070 c         1811 g         3170 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc
     7261 atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc
     7321 tttttcatga cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc
     7381 aaagcgctac tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag
     7441 cggaacagtt tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt
     7501 agcgattttt ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt
     7561 tgtcacattg gaaactactt tatcaatcag gacaaattgg aaatcgccaa cttctcgccc
     7621 tggtgtcaga gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg
     7681 gatgacttgg cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt
     7741 cacttggaca ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc
     7801 atccgtcact tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa
     7861 aatcgaatag atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac
     7921 cacatttgtc tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat
     7981 ataatctctc aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca
     8041 aataaacatc acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa
     8101 gaatttgaca acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac
     8161 taaatatgca agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt
     8221 tgtcatcaac atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt
     8281 tcggaatgtg acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat
     8341 atacatttgc ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa
     8401 cttgattgct tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac
     8461 tgcagcgact gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa
     8521 gaatggattc aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca
     8581 cgcagcctgc ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg
     8641 aatatttcgt ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc
     8701 caggaaaata tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg
     8761 gatagcataa taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa
     8821 tcccattacg cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac
     8881 aatatctgta ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat
     8941 gacgatcacg ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc
     9001 ttcaatcgca gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc
     9061 tcctccgatc attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag
     9121 tgagaaaatt ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat
     9181 tgttgtcagt agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg
     9241 attgattcct gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact
     9301 tgtcccgatg tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt
     9361 tttggtattg tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt
     9421 aggtaagctt gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt
     9481 ctaattatcc aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt
     9541 ggacgttctc tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc
     9601 aataatgctg gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat
     9661 tcgccgagcc aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact
     9721 tcctttccta aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat
     9781 aaaaaattca gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt
     9841 tattcagaag gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca
     9901 aaagtgttca gcttatttcc gtagaagctg cttctggaac accgtgaatc agaggttgtg
     9961 aaatcagcg
//
LOCUS       DANKHL010000104         9951 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000104 DANKHL010000000
VERSION     DANKHL010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.108"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(338..1186)
                     /locus_tag="PW101_001806"
     CDS             complement(338..1186)
                     /locus_tag="PW101_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2514204.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1185..2252
                     /gene="gnd"
                     /locus_tag="PW101_001807"
     CDS             1185..2252
                     /gene="gnd"
                     /locus_tag="PW101_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HDL2514205.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2254..3255
                     /locus_tag="PW101_001808"
     CDS             2254..3255
                     /locus_tag="PW101_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDL2514206.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFNVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMCEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3252..3536
                     /locus_tag="PW101_001809"
     CDS             3252..3536
                     /locus_tag="PW101_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDL2514207.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3569..4366
                     /locus_tag="PW101_001810"
     CDS             3569..4366
                     /locus_tag="PW101_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2514208.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLSSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4380..5213
                     /locus_tag="PW101_001811"
     CDS             4380..5213
                     /locus_tag="PW101_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2514209.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5231..6088
                     /locus_tag="PW101_001812"
     CDS             5231..6088
                     /locus_tag="PW101_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2514210.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6110..6619
                     /locus_tag="PW101_001813"
     CDS             6110..6619
                     /locus_tag="PW101_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2514211.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6656..7057
                     /locus_tag="PW101_001814"
     CDS             6656..7057
                     /locus_tag="PW101_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514212.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7169..7522
                     /locus_tag="PW101_001815"
     CDS             7169..7522
                     /locus_tag="PW101_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514213.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7556..7999
                     /locus_tag="PW101_001816"
     CDS             7556..7999
                     /locus_tag="PW101_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2514214.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            8016..9101
                     /gene="uxuA"
                     /locus_tag="PW101_001817"
     CDS             8016..9101
                     /gene="uxuA"
                     /locus_tag="PW101_001817"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2514215.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9094..9807
                     /locus_tag="PW101_001818"
     CDS             9094..9807
                     /locus_tag="PW101_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2514216.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         3328 a         1569 c         2180 g         2874 t
ORIGIN      
        1 ttacaaatga taacctaaaa tgaggccaaa agccagtgtt atcaaggaaa tacttaattc
       61 accaaaattt tttttaattt tttagaaaat aagaaacaaa aataataaaa aaaagaaaag
      121 ataaattttt tactactgta tactaacttt gttatcgcat ctaacaagta actcgaataa
      181 taccaataca accttctcca aagatgcgat gacatacgtt ctaacccacc gactattgat
      241 gttctgtacc acccttgcag aaccaaaaaa gccactacca aatatttggt agtggctttt
      301 tttgttttat ggatttttcg ctttttctgt tatggtgcta ttctttgatt tcagaaatag
      361 tatgtcgaac tttagcgata gaacgacgtg attctttttc gttgtaaaac atcaaaatca
      421 catatagaga gtccaaaatg cttagttgag aaatacggga agctaaagct tcagatcgga
      481 attctgtttc ttcagaaata gaaataaaaa cgacatcgcc aagtttagca agggaagaat
      541 tagcttggct agtaataacg attgtttttg ctccagtttt tcttacggtt tcagcaatat
      601 ggattgtttc ttttgattgc cctgtgtgag aaatcaaaac agcacagtct ttttccgtca
      661 ataaagaagc ttccatcaat tgaatatggt aatccgtgct gtgagaagtt gcgatagggg
      721 aacgcaaaaa tttatgataa gcgtcagttg ctacaatctc tgaaccgcca acaccgaaaa
      781 aatagacacg tttagattca ttgataatag tagccgcttt ttttagatcc ttttgttgta
      841 acaacttttt tgtgtcattt aaagttgaaa tactagactc gaacaccttt tgcgccatag
      901 tgagctcagt atctgttttc tgaatatttt cgtggataga aatagcagat agatcatttt
      961 cttggatcaa catggccatt ttaaaatctt taaatccatt ataaccaagt ttttttgtaa
     1021 attgaaaaaa agtagagtca gcaatgccca gttcacttgc taaattgcta ataggactat
     1081 gacttacttt gcttggattt tctaaaatat aatcggcgat agcctgttct tttgggctga
     1141 agtcgttgta cattgttttg atttgtagtc ttatggagac gctcatgaga attcctcatt
     1201 ttcataataa gattatcatt ttttccgttc tcattaatgt taaagataca aggagagtag
     1261 gaaaaaatca aacaaaaaat atttttgtaa aatctattta caaaaaaatt tttttgtata
     1321 atgagggtat aagaaatgga ggcgctatca tggaaattgg tgtaattggt ttgggaaaaa
     1381 tgggattgaa tttagcttta aacttaaaag agcaccatca caaagtacaa ggactcgaca
     1441 tttcaagtac agctgttagt caggcaagag aaaatgacat tcctgcttat caagagatct
     1501 ctgaatttct agctactttt gagcagaaaa aaatcatctg gttaatgctt cctgctggag
     1561 agataaccga aaacatgtta agaaaacttt ttccaaaact cggaccagga gacattatta
     1621 ttgaaggcgg aaactcttat tataaagatt caatccgtcg agcagaagag tttcagaaaa
     1681 atgaaatcgg ttacttggat tgtgggactt ctggaggaat cgaaggagca cgacatcaag
     1741 cctgcttaat gattggagga gataaagaaa cttttctatc cgtggaacaa ttattcaaag
     1801 atgtagcaat tgaaagagga tatctgtatg ctggtgcatc cggaagcgga cacttcttga
     1861 aaatgatcca taatggaatc gaatatggaa tgatgcaagc aatcggtgaa ggattccaac
     1921 ttgtacaaaa aagtgagtat gcttttgact tggaacaagt cgcacgtgtt tggaatcatg
     1981 gttcggtgat tcgcggctgg ctgatggaaa tcatggaaga tcagttaaac gaacatccca
     2041 atttaagtaa ttatcgggga atcgtttctg catcaggtga agcaaaatgg acggttgaaa
     2101 cagcgctaga aatggctgtt ccagttccag taattgcaac tagtttgttc atgaggaatc
     2161 tttcacagga agaagatagt ttttcagcaa aagtagtttc agcactaaga aatgggtttg
     2221 gcggacatga aatagttgaa aagggtgagt aaaatgccag attatcatat agcaatcgtt
     2281 aattccagca gtttcggaag agtctttcca caccacttgg aaagattaaa gtcagttgga
     2341 gacgttcatt tctttaatgt tgatcaagag attccaggaa aagaactagc ggagttactt
     2401 aaaggattca atatcattat tgcaagtgtt acgccatttt ttcgacgaga tttttttgaa
     2461 aacaaagatg aactgctatt gatttcccga catggtattg gttacaacaa tattgattta
     2521 aaagcagcaa aagaacacca gacaatcgta tctatcatac ctgctttagt tgaacgaaat
     2581 gctgtagcag aaaataatat aactaatttg ttgtccatgc ttcgacgcac gacacaagca
     2641 caagcaagag tcaaggacga tcgatgggaa gatcgggcgg agttcgtcgg taggacgctg
     2701 tttaacaaaa cagttggtgt gattggtgca ggaaataccg gaagcggagt aatcgaaatt
     2761 ttgcgtaacg gttttcgctg tgatgttcta gcttatgatc cttacaaatc agatttatac
     2821 atcgaaagct ttggtgcaaa aaaagtagaa cttcaagaat tactggaaca atcagatatc
     2881 atttgtttat gtgcgaattt gacagaagag aactatcaca tgatttctga taaagaagtc
     2941 gatcagatga aagaaaatgt ttatctatct aatagtgcaa gaggcgctct agttgatgaa
     3001 gaagcgatga ttaatggtct gaaaagcgga aagatcgcag gatatgccac agatgtttta
     3061 gaagaagaac cgggacgtca agatcatccg tatttagcgt ttgaacaagt agtgattacg
     3121 ccacatatat cggcctacac aagggaatgt ttagaagcaa tgtgtgaaaa atgtgtcaga
     3181 gatgtcgaac agatcagtca gcaccttctt cctgaacgtt ctgtccagac aaagagcaac
     3241 tatataaaag gatgatgaag aaaatggtga acggtttaaa agtgaaaaca ggccctcaat
     3301 tttatttata tgaagaaggt ggtatttcaa aagtttctga tctcttgaaa tcatacggcg
     3361 cgaaaagagt actggtcacc cacggaacgg tatcatggga aaaggcgcta cctaaattag
     3421 tatttttgaa tgacgaaacg attcaatttt tttatcatcg ttattctgga gaatgtagtt
     3481 atgctgaagc cagacgaata gctacaatca tcaaaaaaat gaaatcgatt tcgtagttgg
     3541 cgttggcgga ggaaaactga ccgatctagt gggttatagt gccaatcttg caagaatcga
     3601 tttcggagtc atccctactt tagctagtaa ttgtgctcca tggacacctc ttgctgtcat
     3661 gtataaggag aatggacaag cagaagggaa aacagaacac ttttacagac aagctgcttt
     3721 ttttataaca gatccccaat tagtgattga ttcccctgtt ccatatttca ttgccgggtt
     3781 agcagataca ctagcaaaat ggtatgaatc tgagctcatc ttagaacaag attttttgca
     3841 agatgaatca tttttgaagc tagcacaaga tactgcgaag atctgcaaag aagagatttt
     3901 gaatcattct gctaaagcaa tccaagatat ggcacaaaga aaactgtcat cggagttcaa
     3961 acagctttca gagattatct ttgctgttgc tggtttagtt ggaggattag gagataaata
     4021 cgcaagaaat gtggcagcac atgctatgca tgacgctatc agtaaatata ttccggaaag
     4081 ccatcgtttt ctacatggag aaaaagtagc gtatgggatt ttctatcaac tggcactaga
     4141 aggtcgctgg gaaaaaatag acgagttata tcctttgtat cgggaattag aactgccggt
     4201 ttctctagaa caaatgggat tatttccgaa agataaacag ataatagata aaatggctga
     4261 atttattgat tcgaaagaga aagtgcattt gattcctata gaggtaacac agaaatcact
     4321 gatacagtca atgatggaac tggaaggtta tatcagaaag atctgaagga ggaggacaca
     4381 tggaaacatt aagtgtagta caaagtttgt tgattgcttt atgggtagcc gcggttatgt
     4441 cccgctggtt aggtggagga gccacgttga ccttacgttt ttctcctttg atgactggat
     4501 tagtcgtcgg gttgatcatg ggggaagttg cacaagcgat gattgttaca gccgctttgc
     4561 aaatgattta tatgggggtt ttttctccag gaggatcaat gccagctgaa ccatctattg
     4621 cagcagctat cgcagtacca gtcgcattat taggggatct gtcgccagaa gcagcgattg
     4681 ccgttgcggt gccagtcgga ttgctcggaa gttacttgta tcagtttcgt tttttcatca
     4741 atactttttt aggtaaatat acagacaaag ctgtagaaga tttgaataca aaagctatgg
     4801 cccgatcaat tattctttat ccaacggttg cctcattttt attattcgtt ccgcttgttt
     4861 tcattgcttt atatttaggg gcgccattga tcgctgatgt aatcaaagct ttagagggaa
     4921 cagtcgttat ccacattctt gaagtagtag gtggagggtt agcggcaatc ggtatcgcga
     4981 caactgtata cgttatcggt cgaaaagatt atctggtctt tttcttcctt gcttatttta
     5041 tgagtgttgt tttgaaatct ttagatatta cgatggttac ttatgccatt tttgggatta
     5101 ttatcgcttt gatctttgtc cagtcgcaaa aagggaaaca agtacagcct gccacaagca
     5161 ataacaattc agcatctttt gatgacgaag atgactatga tgacggattt taatttagga
     5221 ggaatgggaa atggctgaaa taatggataa tcaaacaggt gctcctgaag aaatcacgaa
     5281 aaaagatgta acaaaagcct atctgagatg gcattttgca aacgaaattc ctcactcttt
     5341 tgaacgttac ttggcgccat cactactata cggaatgatg ccaatcttga aaaaacttta
     5401 taaagatgaa gactctttaa aagcagcata taaacggcag ttactgttct tcaacactca
     5461 attaagctgg ggtggcggtg tgattactgg cctaatgtct tctatggaac agcaaagagc
     5521 agaagaagag tatgaagaaa aagagatctt gatgcaggat gacttgatgt ataacacgaa
     5581 agcgggattg atgggggcac tagcaggaat cggagatgca atcgattcag gaaccgtcca
     5641 atatattttt atcgcaatcg cggtaccttg ggcacaacag ggaagtcctt taggcgcact
     5701 ttttcctttt atctgctttg ggttgtatca ggttttacta ggggtatttt tcgcaagaca
     5761 agctttcact atgggccgta atgctacagg cctaaagcaa aacactggtg ttcagacagc
     5821 gattgaaatg ctatcagttt tagggttgtt tatgatgggg gtgcttgcgg gaaactatgt
     5881 aaaagtagaa tcttcattga aatttgcgat atccggacgt gagtttgtta tccaggaaat
     5941 tcttgatcaa atcgtgccag ggatattacc tttagctgtc gtaatgggtg tgtacttgtt
     6001 ctatacgaaa aaaggattga aagtcacaca agcattatta tggctgactg gaatattaat
     6061 tgtgctagca ggaatcggta ttttataaaa agaagggaac gtgaataaga tgggtgtagt
     6121 caatttagcg cgtgtggatg agcgtttgat ccatgggcaa gtaatggtaa cgttatctca
     6181 aaaaagtgga gtgaattcca tttttgttgt agacgaagtt gtagcaaaag ataaatttat
     6241 gagagatctt tacaaaaatg caggaagccg tacaggacaa aaaacaattg tcattacacc
     6301 agagaaagct aaattctatt gggatgaata tcaatttaaa gaatacaatt gtattttgat
     6361 cgcaaaaaca gtccaaacga tttacgactt agtaaaacac ggaatcccca tggaagaatt
     6421 gaatattgga gggattgctc aaaaaaatcc tgatacagat cttttagtga cgaaatctgt
     6481 ttatttaaat aaatcagatg ctgaaaaatt gaaagaaatg aatgaacaat atggcgtaaa
     6541 gaatatttat tttcaagcca ctccatctgc acctaaaaca ggattaaaag atgtattgaa
     6601 caagtttgat ttgtcataaa caaatgctta gaaaaaagat aaagagggcg aaaaaatgaa
     6661 gaagaacgag ctgtttcgtg attgggaatt tcgttatcgc tatatttacc gtaagcgccg
     6721 tacaaaaaag agtaaacagc gttttctttc agcattagta tcagatattt acagtatgag
     6781 aacagatgtg acagtcattg cttacgatac gctagcctac cgatcaaaaa atatttatgt
     6841 tggagatatc gaaaaagcag agaaagttat ctgtacttat tacgatacac ccgttcatgc
     6901 attaggaagt tatttcatgt ttgattggaa agaccaaagg aaaaaaacaa tctactctat
     6961 attattgtcc ttcattttgt tgttcagttt aggttggtgg ggaatgatga tatataacaa
     7021 aaatcctcat cacgtattcg atctgctatc cgtataaaca ggtatcactg tactagcatt
     7081 tgggagctac tttttcttat tgggaaaagc agctcgcgga tggcctagca gacaaacttt
     7141 tattcgaaat acttcatcca tattaacgat gctagaaatg atccgtacga ttgatgatcc
     7201 gagtgtagcg tatgcgtttg ttgatgaagg ctgttatgga gaaaagggct tagacagcgt
     7261 gagatcgggt atgaaaaaag aggctattct tttttatcta gactctgttg gggcagatac
     7321 accgctacag ttttcgggaa attatttttc aaataaagaa cagtggctga aacaagttga
     7381 taagttgaaa gaaaaaaacg tcaattatat ttttagtgca agaaaaaagc aagcgcagtt
     7441 cttctatttg accaaaacag atttacgagg gaaaacattt aattggcaaa atgccaatca
     7501 gattattgct ctttttcgat aagtaaaaag tgcaaaacaa ggagtgagaa gactaatgtt
     7561 ggaaatcatg atcgttacac atggtaagtt gagcgaagga ctgaaagatg cggcggaagt
     7621 aattgtcggg acatcgagcc atattgaaac aatgtgctta gagtctgggg aggacatcca
     7681 aacattcgga gagaaggtca aagaaaaatt aactatgatc aacacagcag aacccatgct
     7741 agtatttgtt gatctaatta gcgcaagtcc gtacaatcaa gcgctcttgg cagtcaatga
     7801 actgactgaa gaaaagaaag cagccactta tgtcataggg ggcgtgaatc tgccaatgtt
     7861 gttagaagca atcaatcatc agatgcttca cacaccgata gaagaagcgg tggaacaaat
     7921 tgtcaaacaa ggaaaagata atttaactgt ttggcatgct tcagcggaag aaaaaaaatt
     7981 agatgaggac gatttttaga aggaaggctg aaaagatgaa gtggggattt agatggtatg
     8041 gtgaaaaagg ggattcgatc ccattaaaat atgttcgcca aataccggga ataaatggga
     8101 taattggaac tttgctgact aaaatgcctg gagatgtttg ggagatttca gaaattgagg
     8161 agttgaaagc atctgttgag agccaaggac tggaactgct tggaattgaa agtgtagcga
     8221 tccacgatgc aatcaaggca gggacagatg aacgagatta ctatattgat aactatatac
     8281 gaacaatcaa aaacttgtct cgatgtgggg ttcgattgat ttgttatagc ttcaaaccaa
     8341 tttttggatg ggcgaaaact gatttgttta atgaaaacaa cgatggaagc tattctcttt
     8401 tatacgatca gaaaatcgtc gatcagatgg agccaggaga aatgtatcaa ttgatacaca
     8461 gccagtcaaa gggattcaaa ttgccgggtt gggaagaaga acggttgaaa aaattcgctc
     8521 agctggaaaa actgtatgaa ggaatgaccg aagaagaatt atttgaaaat ctgacttatt
     8581 ttttagaaaa agtgattcca gtgtgcgaaa aagcagatgt aaaaatggct atccatccag
     8641 atgatccgcc atgggaactt tttggttttc ctagagtgac gaaaaatctt ggcgacttga
     8701 agaaaattct aaatgcagta gattctccat ataatggact aacgctgtgc acaggatctt
     8761 taggggcaaa tccggaaaac gatttagttg aaatcatcca tgaggtaggt tcgcgcatcc
     8821 attttgtcca cttcagaaac gtggagtttt taggagaaag aaagttcaaa gaaacggcac
     8881 atccctctac agaaggctcc cttgatatgt atcatattat gaaagcgttg gtgcaagaag
     8941 ggttcgacgg tcctatacgc cctgatcatg gtagaaccgt gtggggtgaa gtggctatgc
     9001 ctggatatgg cctgtatgat cgtgcaatgg gactcactta catgcaaggt ctatatgagg
     9061 cgataagtaa ggaaaaacca ctgggagagc attatgaata aaatcaagct cttcatcttt
     9121 gatatggatg gccttttatt agaaactgga cgtttagctt atcgtgctta tgtaaaatca
     9181 gcacaaaagt atgattatga aatgcgtaag gaagtctatt atttactaac tggccaaaca
     9241 gaaatggcga taagagaaca aatgggcatt ttgtacggag aagatgttcc atatatcaaa
     9301 tggcgagaag caatcaatca atataaagaa aaaatcgtga aagaagataa gagagtatat
     9361 acgaaaaaag gagcagaaga aattttatct tttgcaaaag aaagaggaat ccatacaatc
     9421 gtggcttcgt ctaatacacg ggtaaaagta gaaatgtatt taaaaatgga aaatctatat
     9481 gccttgtttg atcaaatcat ttcaggggat gatgtaaaaa aaggaaagcc ggaaccagag
     9541 atctttttga aagcttgttc aaaaatgaat attcctccaa gtgaagcact tgtatttgaa
     9601 gactcgattg cagggatcga agcagcaagg cgagcgggca ttttgtcttt tctcatcaaa
     9661 gatcatttga tagggttgcc cgatcataat gggaagcata agttgaaagt agatgtaagt
     9721 ttatatgaat ccaataaagc agactatgaa ttttatgacc tgcatcaagc aaaacgattt
     9781 ttagaaagta aagggttgta tctctaataa agaggactat tttctagtcc tctttattta
     9841 ttgtcctgca cgactgaggg aacagatgaa aaagaagtaa ttttttattt tgaagtatct
     9901 accttatttg tagtgaacaa tttagaacaa acacaaaacg catctactgt t
//
LOCUS       DANKHL010000105         9722 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000105 DANKHL010000000
VERSION     DANKHL010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.109"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            88..3366
                     /locus_tag="PW101_001819"
     CDS             88..3366
                     /locus_tag="PW101_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HDL2514217.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3907..5814)
                     /locus_tag="PW101_001820"
     CDS             complement(3907..5814)
                     /locus_tag="PW101_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HDL2514218.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5944..6819
                     /locus_tag="PW101_001821"
     CDS             5944..6819
                     /locus_tag="PW101_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HDL2514219.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6899..7873
                     /locus_tag="PW101_001822"
     CDS             6899..7873
                     /locus_tag="PW101_001822"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL2514220.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8053..9198
                     /locus_tag="PW101_001823"
     CDS             8053..9198
                     /locus_tag="PW101_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2514221.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9335..9679
                     /locus_tag="PW101_001824"
     CDS             9335..9679
                     /locus_tag="PW101_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HDL2514222.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
BASE COUNT         3169 a         1795 c         2054 g         2704 t
ORIGIN      
        1 ttataatttt gttttttatt gacagtaaaa ataaattagg taatacttta agtaaacgct
       61 ttctaatttg ttacgggagg aatacacttg ttaaaaaaac gtataaaata cagtgcgcta
      121 atgatgaatc tccttatgct cagtaccccg gttttaattt ctgtcgttga agttgctgct
      181 aacgaacaac agtctgtgaa cgaagaaaat gcacacgtga acattgaagg gatgtcaaca
      241 atggaggaaa taccaagaac tagtgaagat atggatacgg aagaaggaaa tgatcctatg
      301 atgaaagaat cagaaaatga aaagacaaaa aagcaatctg aagaagcaaa tgaagagtgg
      361 aaaggggttg ttttcggaga aagcacaagt gctagtaggt atgccattga accattagaa
      421 gacggcgtgc ggctctcttc cacgaataac ggcggaaaat tccagtcaag cggatcagat
      481 ggaatgacct attattatca agcaatcccc aaagacatca actttaggat gcgggcgacg
      541 atcgaaatag aatcttggac ctatacgaat gcccaagaag gatttgcttt aatggtaaga
      601 gacgcagtac caaaaaatca tttgtttggg caggcatttt attcaaattc atttgccata
      661 ctaggcagtc ggatcgaata tgtctgggac tcggatagac aagaagtggt caacacgagc
      721 ggcagcaaat atacgatgcg acttggattg gggacacggg caatcacagg aatatcatct
      781 gaagatccgc aagctgcgcc agctcctggg agtgtctcag tggaaacaac acctttagag
      841 acttcagcag ggttcttgga aaaagaaaca ggttcttata atatattcgg aaacggtcat
      901 gggaatctgt ttccggccac gaactcgatt actaaactgg acgtagagat gaaaaaaacg
      961 aatactggtt tagaggcata ttattacgat cctgaaacag gagaagaaat ggcttctgac
     1021 atcatgtatg actgggaaaa attatacgct tcagatgaat cggttattta tgcaggattt
     1081 gctgcagcaa ggaatatgac gatcaaggta gaaggaatcg aagttgctta ctcagatcct
     1141 gcgacagatc ctcctgggca agaaagacct actcgcttga ttgatccgat ttacactgtg
     1201 acctctagta atcattcagg aaaccaagat catactatgt cctttagagc aaatgctgac
     1261 ggattattga caattaaaaa taaagcaaca ggagaggtgc ttaagaacgc aaaaaacttg
     1321 gctatcactg cgaatcggga atttgattat cagacaaaac tgatggaagg aacgaacgaa
     1381 tttactttca gttttacgcc agataaaaac tatccgccag aagaatacgt agagatgact
     1441 agttatgaaa caaaagagat tcttcataca gtggactacc gtaaacctaa atattcgaca
     1501 gtttatgtaa caccagaagg gtcagatgca ggaaacggaa gcagacaaaa tccattatct
     1561 ctgacacaag cattgagata cgcatatgct gggcagacaa tcgtcatggc gcctggaact
     1621 tattccttcg aaagaggatt gacgattcca aaaggcgtta acgggacaag agaaaacccg
     1681 attcagttga ttgcagagaa aaatccagaa aacaagcgtc ctgtacttga ttttaaagga
     1741 actggaaatg ggatgacact gttcagtcac tactggggtt tacgagggtt tgacattaca
     1801 aatagtgcag ctatgcaaaa aggattacag atttctggaa atcataattt gatcgaagag
     1861 attcatgctt atcggaatgg aaatacagga atccaaatca gcggtagctc aaatgatcct
     1921 ttcgaagcat ggcccgcgca taacttagtc aagaactgta catcttttga aaatgccgat
     1981 ccaggatttg aagatgccga tggatttgcg gccaaactga caataggaga aggaaatgta
     2041 tttgacggtt gtatcgcgta tcataatgca gatgacggtt gggacctttt tgctaaaaat
     2101 gaaacaggtt cgatcggaaa agtaatcatc aaaaactcag ttgcttatcg aaacggctgg
     2161 gtaccaggaa ttgagggaga aggtaacggt aatggattca aaatgggcgg ttcttcttta
     2221 acgggccctc atgaattgat caatagtttg tctttcgaaa atcttgcgaa aggaatcgat
     2281 tccaatagcg gaacagatat tatcgtcaat agcagtacaa gttttaataa cggttcatat
     2341 aatgtggctc tttatacctc ttcagcagga aacacagact accatgcttc tggtatctta
     2401 tctttccgaa cagagaatct tgagatgaga gagcagatcc gcccattgaa tcaaaacgaa
     2461 gatcaaatct accactcgtt gaactactat tggaatgaac gagagaaaca atcagaaaat
     2521 actcttggac ataccatatc aaaagattgg atcgagtctc taaatacatg ttcgaaagaa
     2581 ggtcagcagc ctgttacgcg ctatgaaaat ggaagtatcc atgttcatgg actgatgcag
     2641 attaaaccgg ggaatcgtca aacagaaaag gaaagagtgg atggtcttcc attatctgta
     2701 ggtgctattt ttgacggaga aacctcaccg tcttcggaat atccagagta tgcaatcgta
     2761 caagaaccaa atcagacaaa accaaatgaa caaaataatc cgattattat acaagaagca
     2821 aatggtgtgg cggagaatgc agaaggaaat atcattgaac tgaagccttt tacagttttt
     2881 gtgtcaactt tcacaccaaa tcaaggaacg cctgtctttg aagatatgcg ttatttccca
     2941 gaaaagatcc aaatcgttga aacacagtca agtgctcaat tttctaatca taccgctatt
     3001 ttttcagcac cttcaaaagg agaatatacg ctagaagtca cttatcgttc ggaaatattt
     3061 gatggggaaa aatggtctgc tcatacagaa tctgttaccc agcaaaaagc gatcactgtg
     3121 cgagaaactg ccgatgtgcc cccgggaaat gacaaaccag aaagagaaaa tccaggaaat
     3181 gaatcaccaa atggaaaacc aaatggaaaa ccttctcatc cggataggac agcaaaagaa
     3241 agtgaaaaaa atggcactca aggactccca aaaacgggag aatttaataa cccgttgcta
     3301 gcattagctg gaggaatttt gttaatcggt gtagttgttt atgtcatgaa acagcgaaaa
     3361 aaataagtgg aatttctcct tttatgatgt aaaacaattg actgaggaaa gtaaaaatag
     3421 aaggaaatga gaccaataga tgtctcactt ccttctatct ttttatagat gaggttctcc
     3481 ttgctgcttt tgattggctg ttcttattac ttgatgcagg acagcttttt cacaatctct
     3541 ccactaaacg gtgttcaatc agctgaccct cggtattagc tcaacaaacg gcaaaatatg
     3601 aggaactatt tttccgtttt tttctctaat actcaggtca ggacgctgat ttcacaacct
     3661 ctcgataaac ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg
     3721 tggaaagcca ttttcacaaa atttttctta ctacttgaaa ctaagcagct ttttcacaac
     3781 ctctaataca tggtattcca gaagtaactt cttcgaaaaa taagccgaaa tgtcacaaaa
     3841 atttgagaag cgattttccc aaattccttc ttatttcttg aagctgacca cttctggcac
     3901 aacctcttac cagattcgca ctcttttttc tggcgctaga tacatagcgt cttgttccgt
     3961 gataccaaat gtttcataga attcgtcaag gttttttggt tgcacgtttg ctcggagttt
     4021 agcaggggca tggacatcga tttgcagtaa aagttgttgg tattcttttt ttgcttttgt
     4081 acgccaaatc gttgcccaat tgacaaagaa ttcctcgata gaaagatctg attccgtttt
     4141 tgctgcttcc aatgcacagc ttagaccgcc tgcatcagca atattttccg aaacggttaa
     4201 ttttccgttt acttttccgt cagcaaaagg cagaccatcg aattcttcga tcattgcttg
     4261 tgctttgttt tgaaattgtg taagatcttc ttctgtccac cagttgttca agttgccata
     4321 ttcgtcaaat aaagcgccgt tattatcaaa tgcatgggaa atctcatgag cgataacagc
     4381 tccgattcca ccataattag cagagcttga ttgttctaga ctgtaaaatg gtgcttgaag
     4441 gatcgctgcc ggaaagacga tcacattccg aaatggatga taataagcat tgaccgtgtc
     4501 agcactcatt tcccattctg aacgatccac tggtttgttc catttgctga aacgatcttt
     4561 taatgtgagt ttgctgaaaa caagagtatt tgaaagcaaa gtaccaccct gatctgcagg
     4621 tactgttttg aactggtcga ataatgctgg gatcttatcg gggtagccta cttggatacc
     4681 taagttattt aattttatga cggctttttc ttttgttgca tccccaagcc agtcattttc
     4741 ttctagacgt tttttataaa cggcgatcat ttttttcacc atttgttcta cgtctttttt
     4801 agcagcttcg ccaaaatatt tttttccgta gtaatcgcca actacttgat cgaattgtcc
     4861 agaggcaagg taatacgctg cttttttctg aggcattgct tcgtctgttc cagacaatac
     4921 gcgagaatag attccgctga cttggcggaa ttcttctgat agataaccac taagagaacg
     4981 tactgtttta atgatcatcc agcttttgaa taacggaaaa tgagcttctg tcaggatgtc
     5041 attcaaatga tcgaagtaca ctggatcagt tacgatcact ttatctggag ttgtcccaat
     5101 caaacccgtg acaagttttt tcaagtccag tttatctgtg tatttaataa atgtatcaaa
     5161 agattgcgga ttatacattt tgctgtaatc tgcgttttct tctgcacttt ttacatgagg
     5221 agcaatcaat ttatcaaatt gcactgcttg ttccgtgatt gcttctgcct gacttttttc
     5281 tttgccggca agctctaata actgaacagc catatcaaag aaaacgccta ttagttgtgc
     5341 accgttggga tggtctgatg cataatacgt cttatctgga aggaataaag aaggcggata
     5401 agcaaagaag gcgttcgttt gcgcattctt catatctgca tctacatcca gattaaatgg
     5461 caatggtaat gtatccaaca gccattctgg aaattgctta ttcaattcgt cgaaagactg
     5521 caatgattca actttttcga ttataggttg aagcggttga gcatgcaact catttcttcg
     5581 gtcataatcg tttgccagtt gataatatgc aaggaaatgt ttcatttttt ctgaagacac
     5641 tttttctgga tgggcaagca gatcgtctgt ttctcccatc aaaagttggt cgatcccgtc
     5701 aactaaatct tggaaaccgc ctgtagctgg tttatctgca gggatttttg ctgttttttg
     5761 ccattcttca ttgactgctt catagaaatc ttgtttcaat cgttctttgt tcattcatcg
     5821 atcgctcctt ttttgtttcg tgtgcccatt atcttacaaa atgaaggaat taacaaatag
     5881 aacataaaaa attgaaaatt cgaacaatca aactgatttg tttttttcaa tccttcactt
     5941 ataatgaaaa aaaggaggcg atcccaactg aatcaagtag tggataaacc tttatatttc
     6001 aaagtagata aaaaaattaa aaaattagcc agcactcagc agctgcagtc aagaaaaagt
     6061 aaattgcttt ttttagctct ggtttttgaa gatcaaagtt atgtgattat cgatcaatca
     6121 ggacatccgg tagaatattc tccagcaaaa tatacttatc aagagggtct ttcttgtaaa
     6181 aaatggaagc tgatcaatga agagccaatc gagttatcta gatggatcaa cagaaaagaa
     6241 gatattcccg ttttgatcga agaaaaaagg tcaggaaagg aactcgcaaa ttgctgggtg
     6301 ggattgccag aagaacgttt tttgaggtat aagaaatggg ctaccccctc aggctatctt
     6361 tgtggtactt acgcggcagc agttctcctt gcctattatc aagactatcg taaggggtgg
     6421 atgcttccaa atgagattcg caagaaaaat acggctgatt cgttggtttt gacgaaggca
     6481 ttgcgtagcc agatccaacc actaggactt cctacgattc cttttcaggt tagtacaggg
     6541 atttctagtt ttttaaagaa gaatggcaat catgagcgtg cacgagcaac tcttctaggt
     6601 tcatggcagc gagctacaaa acgtatacgt gaaggcaaac ctgtgatgat tggtattttg
     6661 aaagtacttg gcagtacata tggcaatcat tgggtgacag cgtacgctta ttttgaaact
     6721 gaaacaggag aacgttatta caaagtacac gacaactggg gcgattatca taaagtcatt
     6781 cctgccagtt ggagtaacgg aacagtttca cttccttaag aatttgctga aattaacaga
     6841 aaatcttgga aaaatatgtt aaactatcga agaatagaga agttttgggg aggactacat
     6901 gagtaataag tatcaggatg acacgttgaa gatttttagt ttgaatggca accgtccatt
     6961 agctgaaaaa atcgcgaaag tgttcggaac agaattaggt aaaagcgtcg tgaagcaatt
     7021 tagtgatggc gagatttcca tcaatatcga agaaagtatt cgaggggatc atgtgtatat
     7081 cgttcaatca acgaatgcac ctgtcaatga ttactatatg gaactactga tcatgatcga
     7141 tgcaatgaag cgagcaagtg cgaaaacgat caatgtcgta ttgccttact atgggtatgc
     7201 taggcaagac cgcacggcga aacctcatga accgatcact gcgaaattga tcgctaactt
     7261 gatcgaagaa gcaggggcaa ctcgagtact gactttagat ctgcacacgg ttcaagtaca
     7321 aggattcttt gatattcctg tagataattt atttacgatg ccgttgtttg ctcattatta
     7381 ccgtcaatta ggtctgacgg gagatgacat cgtagttgtt tcgccaaaaa acagcggtgt
     7441 tcagcgtgca cgtagtcttt cagaatattt gaacagcaca ttagccatcg tagatcatgc
     7501 agatgatgct tcaactgagg gtggttatgt gatcggtgat gttcaaggaa agacatgtat
     7561 catggtagat gatatcttga acacaggagc aacgcttgca cgagcagcaa atgtattaaa
     7621 agaaaacggc gcaaaagaag tatatgcctg tgcgtcacat ggattattgt ccgatcatgc
     7681 gaaaagtatt ttagacaatg caccaatcaa agatatttgt atcactgatt ctgttttcac
     7741 agaagaaaat cgtcatccag aaaacttaac aatcgttaca tgttcagaat taatgggtga
     7801 agcggtcaaa cgcatccatg aaaatacacc gatgagtccg ttattccgtc tggaagaaaa
     7861 agaattcgaa taaacaaaaa aaagagggaa agaagttgtg acagaagtga gcagcttcaa
     7921 ggagctgcga caagactttt gtcacagctc cttttttcta tgcccatgaa acgtcctcta
     7981 gacaaaaatg agctcttctt tggtttgttc gctattttat gctagaatgt gcctagcaat
     8041 aatttggaag ggatgaatga agtgaaaact atttatctgg atcatgcggc aactaccccc
     8101 atgcatccac aagtaattga agcgatgacg gcaatcatgc aagacacgtt tggaaatcct
     8161 tcaagcatac atggatttgg tcgccacgcg catgaaaaat tggaaacagc aagacaaatc
     8221 attgcagaca gtctgcaagc aaaacctcac gagatcatct ttaacagtgg gggaacagaa
     8281 ggggataata cagctatctt agaaacagcg ttctcgcgtc aaaaagaagg cagacatcta
     8341 atcactacag ctatcgaaca tcctgctgtt cttaatacca tgaaatattt agagacaaag
     8401 gggtttgacg ttacatatct gcctgtagat gaaaaaggga atctgtcgat cgaagaggtc
     8461 aaaaaagcgt tacgagaaga tacgatcttg gtctctgtga tgtacgggaa caatgaaatc
     8521 ggaaatttga tgccaatcaa agaaatcggt gaacttttag cggatcatcc agcttatttc
     8581 catacagatg cggtacaagc gtacggaaac caaacgatcc atccagaaga attaggaatc
     8641 gatttattaa gtatctctgc tcataaaatc aatgggccaa aaggcgtcgg tttcttatat
     8701 aaacgagacg gagtaaatat ccctgcattg cttttaggcg gggagcagga agaaaagcgg
     8761 cgtgcaggta cagaaaattt agcagggatc tggggtatgg caaaagcagt tgaactgctg
     8821 tcacctgaag aacgtgaagc acgtaacaac aaatataaag gattccagca attgattctt
     8881 gatacattag aagcaaacga aatcccttat caagtaaatg gtgatttaac gaataaactc
     8941 cctcatgtgt tgaatcttcg cttaattggc gaagcaaatg atttgctact gatgaagctt
     9001 gatcttaatg gggtcgctat ttctactggt tcagcttgta cagcaggaaa tatcgagcct
     9061 tcacacgtat tagaagcaat gtatggagaa gatacaccga tactaaaaga atccatccgt
     9121 atcagctttg gtttgggaaa taccgaagac gacatccagt atttccttga tcaattagtt
     9181 cgtgctgtca aaaaataact agtgaagtca ttcttatatg aacagatgtc tgagagaacg
     9241 ccttcatttt ttagggaact taactgaaaa acctagtaaa aatcaggata aacttttata
     9301 ataaagagaa actaatttta tcgaggtgaa aatcatggca tttgaaacaa cagcaacggt
     9361 actgggcgca ccaattagct atcgtttgca tcctgatgca aaaagatata cattgcgcga
     9421 taatggattt acagaaacaa acggaggcaa ttatcaattg atccgaccat tagatgccac
     9481 gccgcaaagc aaagaaggat tcaaattaaa aatcactgta tcaaaagata ttcaaacatt
     9541 gaaaatgtcg atcactacag caaatggatt gaaagcagtc aatattttca aagatccaaa
     9601 acagaaaatg agccaagata aattttattt tctgatggat gggatgatta gccgtgggtt
     9661 attcgaaaaa gtcgaatgat ctgtggatat aaaaagacaa ctagttttga aacggcctat
     9721 tg
//
LOCUS       DANKHL010000106         9649 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000106 DANKHL010000000
VERSION     DANKHL010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.110"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..190)
                     /locus_tag="PW101_001825"
     CDS             complement(<1..190)
                     /locus_tag="PW101_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514223.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLF"
     gene            complement(206..820)
                     /locus_tag="PW101_001826"
     CDS             complement(206..820)
                     /locus_tag="PW101_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDL2514224.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(967..1650)
                     /locus_tag="PW101_001827"
     CDS             complement(967..1650)
                     /locus_tag="PW101_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL2514225.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1759..2217)
                     /locus_tag="PW101_001828"
     CDS             complement(1759..2217)
                     /locus_tag="PW101_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDL2514226.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2301..4064)
                     /locus_tag="PW101_001829"
     CDS             complement(2301..4064)
                     /locus_tag="PW101_001829"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDL2514227.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4285..5745)
                     /locus_tag="PW101_001830"
     CDS             complement(4285..5745)
                     /locus_tag="PW101_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514228.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5953..6879)
                     /gene="glsA"
                     /locus_tag="PW101_001831"
     CDS             complement(5953..6879)
                     /gene="glsA"
                     /locus_tag="PW101_001831"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDL2514229.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6902..8329)
                     /locus_tag="PW101_001832"
     CDS             complement(6902..8329)
                     /locus_tag="PW101_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2514230.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8360..9574)
                     /locus_tag="PW101_001833"
     CDS             complement(8360..9574)
                     /locus_tag="PW101_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDL2514231.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2852 a         2021 c         1607 g         3169 t
ORIGIN      
        1 taaacaatcc gctaatcgtt tgatctggca tttctatttc cttaccatct aaagtaacag
       61 tagtaacgac aaaatggtct tgcaatggcg gaacttctaa gtactgccct tgcagaaaat
      121 cattcactgt ttcatattct tttttcattt cttttgaatc tgtcgatggt ttatagctta
      181 tttttaacat aactagtgct cctttttatt cgggacgtgc aatcgtgaaa atttctccta
      241 ttgctgcata atcatttcct gccaaacggc ttacagcatt caattttctt ggatcgatcc
      301 ttcctgtctt atcatcatag actgtttcat ttaaatgata tttctgtact tctagcaaaa
      361 ataaatcagc tgttacttta tttttattcc gtatctcgac atgttcatat aaggatgttt
      421 caaaacgaac ttttgcttct gtcaatgctg gtactgaaat gattactgaa tcagttaaag
      481 tcaagtcagt caggtacagt tcgctttcct cattaggcag attggctgct gttttattta
      541 cttcttctac gttgtcctca tcaaggatat ggataacagc ttctcttgtc tcaagaatat
      601 tctttgctgt gtcttttact gcatcttgtt ttctctggat cgccaaggat aagatgggtc
      661 gattcgaact gacgatattg aagtaactga agggtgcgat attcactata tcttttgctg
      721 atttagtcgt tactactgct actggtctag ggatgactga accaatcagc aacttgtaat
      781 tttcacgttc tgtcaattct gaaggactcc gttcaaacat ggtttcgcct tctttctata
      841 aaattcgtta aaaagcagat cttgaagaca aaatgaaaag taatgctttt ttcatcttct
      901 tagaatctgc tttaaactat ctatcaattt ttcttttttg tcaagaatct gtgccgtgcg
      961 tgttttttag ttatttgctt tacttgcggc ttcgacatat cttttagtga tcaattgcgg
     1021 acgggtgata atagatccaa ctacgactga aaaagcacct aattcatatg ctttctctac
     1081 ttgttcagga gagtcagtat gtccttccat aattactggg atatcgactt gttctatgat
     1141 ttgattcaat aaatcaaaat caggtgtctg cgtctttcgt gaatattctg tatagcctgc
     1201 taaagtcgta gacaaaagat caaaaccatc ttcttgcgct tgaagcgctt cctcaaaagt
     1261 cgaaatatct gccatagcta accgaccgtg ttcgtgaatc atttggacaa gttcagcagt
     1321 tgttttttct gtcggacgtt tacgagaagt tgcatccagt gcaatgattt ctgcttgcgt
     1381 ctccaaaagt tcttccactt ctttttctgt cgcagtaata aacacttctg acccatcgta
     1441 atctcttttg ataatcccta ttaccggtaa atcaacgatt cgcttgattt cattgatatc
     1501 ttctgtcgta tttgctcgaa taccaactgc tccgccttct tttgcagcca aagccattct
     1561 ccccataata aaagaactgt gtagcggctc gttttccaaa gcttgacaag aaacgacgac
     1621 tccgcctttg atttgttcca gtgtatccac aatatctcct ccagtaatgt tatataaaaa
     1681 tacagcttca tataaaaatg taaccctttt ctattcagga gacaataatg agctatttct
     1741 ttcagcagac ctttcttact aaaagaaaat tacttcaaat tcttcgtata cgaccagctt
     1801 ctcttctgta aattctatgt tcaacgcttc tttataagca tgtttaaaaa actcagcatg
     1861 gactttccgc caataagcta atgtccgatc cccttctcct tctttccaag catgctcttt
     1921 cgtgacttca ctaaaaggca agatggtcac atttatattt ttgatgatac aaactggttc
     1981 attttttcca ttcaggacca cgtctaattg accaactttt ggtaaaggct cattctcttg
     2041 tttatacaga tcgtaaccac ttgatgtacc agtctttatc ttttttacaa ctagttcgct
     2101 caactcgtct gccatttttt ctgtatcccc aaatgaaaag aaacctaatt tttctgattg
     2161 ataactagga tatttttctt ggaacttttt gacatattct tctacttgct gatacataac
     2221 atccacccct ttgcttttta taatgaaaaa accatccaga tactagtata aacaattatc
     2281 tggatggtga gcataatgaa ttattgaata ttcaagacac gcagactatc ttcaacgatt
     2341 tgttttggtg ttaaatgata acgttcataa agctgcccta caggaacgct atccgtgaat
     2401 tctttctctg caccaaaatt taatacgttc attgaagaat caccataata acgactgata
     2461 ttttcgccaa atcctcctga caaactacca tcttctaatg tgatgactaa ttcgtgtgtt
     2521 tcttttaatt tatctaatgt ttctacgtca ttttctgtga tcgttcctgg attgatcaac
     2581 gttgcttcga ttcctttttt tctaaaagct tttactgttt cttttcctaa ttcaagaaaa
     2641 ccgcctaaag ctaaaattgc tacttttcct ccattattta ctgtttgata ggctggaata
     2701 ctataatcat ttgcgatagc tgtaccatgt tctacaccat gtgtaggaat tcggattgct
     2761 acaggctgcg ctgtttgttc gaacgcccaa tctagcatag agatcatatc ctcttttgtt
     2821 gctggagcaa gatatttgat gttcgggata cttgataagt aaggaatgtc aaatgttcct
     2881 tggtgtgttc ggtcactagc tgagatcgtt ccgccaccga ctaaaataat cactggattc
     2941 tcgttgatcg ctaaatcatg tgataattga tcatatgccc gttgcaagaa agtacttgat
     3001 tggaagacga ctggacgtac tccctgagaa gccaaagcag aagcgaaagt gatagagtgc
     3061 tgttcagcga tccccacgtc aaaataatga tctggatgtt tttcttggaa tgttttcagt
     3121 ccaaaggcgc ctggtatcgc tgcattgact gcggcaatag gagctgtact tgtttgcatt
     3181 ttcttatcca aataatccat gatgatcata ttaaatgttt ctccagaagt tacttgcttt
     3241 gcctccccgg tggacagatc aaatggtaca tgccaatgat atttcatttt attatcgatt
     3301 gccggctgat aaccgcgacc tttttccgta tggacgtgaa gtacaatcgg atgatcaata
     3361 tcttttactt cttcaaataa acggatcacc gtttccagat cgtttccatt atccacgtaa
     3421 cgataatcaa atcccattgc tttaaatagg ttattgctgc ttttaccgtt ggtttgacga
     3481 agctcagcta agtttttata taaactgcca tgattctcat caatagacat ctcattatcg
     3541 tttactaata cgattagatt ggattgtaaa acaccaccat tgttcaatcc ttcaaaagct
     3601 aagccaccac ttagggagcc gtcaccgata aatgctacca catttcctga ttctttcttc
     3661 agatctctcg ctttagctaa acctgttgct aagctgatag atgtagatgt gtgccccact
     3721 gtaaaatagt catgtttact ttcatgttgg ttggaatatc cgctcacttc acggtaatgt
     3781 cctttttcaa aaccattttt tcgaccagtt aaaagcttat gaccataaga ctgatgggaa
     3841 acatcccaaa cgattttatc ctttggtgaa tcaaatacat aatgaaacgc aatcgttaat
     3901 tctactacac ctagattcgg accaacgtgc ccacctacat cgctgtcctt ttgcaagatt
     3961 ttttggcgca tctcttctgc caactgtttc atttcctcaa cagataattt tttgacatct
     4021 gcggggctat tgattgcatc caatacatgt aatactgtac tcataataca ttctctcctc
     4081 cgtactatct tcttttaatg aaaatagatt tgttacactt tatctattct actacttaga
     4141 gttgactcta agtcaatagc attttaaaaa aatttcagag aaagcttgtt atataagtag
     4201 atcttcgcac gaatcaggac tcaaataaaa accttctaca gacaattaaa aagctttgta
     4261 gaaggtctcc taattaaggt ctcatcaaga aatatgtttt tttaataggt gaagttcctg
     4321 agcaatatga tacaaatgga tgtttgcacc ttttaatgag attggataga tctttgtctt
     4381 ttcagtttct aatccatttt gatagctagc agcatcgcac aaaaccactc tttttttcgc
     4441 acatagacat tcatcaggca attcttctaa tgaacgaaat aaaataggat ttatttcagc
     4501 gatgccttct tccgcataca aatgaaattg ggttcgataa aaaaattgtt tgactgaact
     4561 ttctgaaaca acaaaaattt ctatttcctg tctccccgct tgttcactgt tgatcaaaga
     4621 aagacttttg acgaattttc gaatgaaatt ttcattccat cttagttgga tttgattttt
     4681 ctttttccac tcttccaata tttctacaat ttttccgtac agaagttgtt ctttttctga
     4741 tagaagatag atcctttccg gtaaaaatac ttgtccatct gcccacaacg tacggacaaa
     4801 ttgtaaaaag gattgcttga aaataaaatt ttctgcatcg tcgatttcag cgatactaat
     4861 aatctgtaaa atcaattcat tcagtgatag gtcattgaga acaaaatttt tataaacaac
     4921 ttcgaaatct tttcttaaca gctccatgtt ctcattggta taatttctac tgttgaacaa
     4981 agaaaaaata taatagacat cttcttcttg taagtctaga attccttttt gctcttccaa
     5041 tccttgacag actaattgaa ataacggctt cgtttctgcg cttttcttat cttcttcttt
     5101 cagtccaagg ggaaatgcct ttcttccgat tgcgatctct attcctcgta tgataaagga
     5161 cttttcatct tccgaatatc ttcttttaaa aaaatgagtc tctacataat caatcaactc
     5221 gttacaagct tcttctacat acggattttt attttcatag ccttcccaat tgacataacg
     5281 tactaaagct agcaagatgt tccgcgagtc aaattctggg ataatgattt ctttattttt
     5341 caaataatca ttttctttaa aaaaatccaa tacgattttt ttgattgtat atactttgct
     5401 caaagaaata aattcaagat cagccagttc tgtactcgtg aatataccat taaaataata
     5461 acttaaaaac tttaaaaaat aagattgacc gtacagaaac tgtgtgagct cagaaagagc
     5521 aataccatgt ttgaatacga gtttatactt atgcttctct cttacttgtt cttttataca
     5581 atattctaga cactcggcta gactttctag atcgtgaagg atcgttggtg tagtcaccct
     5641 tagagaaata gctgtttgtt cgatgtctat ttcttggaat tcaaataatt gttcacacag
     5701 atacactttt ctaaatatat ttcgctcgat gtaattttcc agcattcctg atacacgcct
     5761 ccagtttttc ttatgatttt atttaatcat atctgtaaat cgtatttttg tttccttttt
     5821 atttagaaaa gccctttcaa aaaccaataa aaaaagaaac tgggacaaaa gtcaaacaag
     5881 ctcacaattt cagcctcatt gtaggtagct ttgagccgtt tggactttta ccccagcctc
     5941 tttcaagaat ggttattcaa agatgtcagc gtctagttct ttgactacat ctttcaacaa
     6001 ttggatacca gctgcgctgt taccaaatgg atctagtgcc gggctgaaga cacctatgcc
     6061 gtaacgattt ggcacagctg ccatcaaccc gccgcctaca ccgctttttg caggtacacc
     6121 aacatggacg gaaaactctc ctgattcatc atacaaacca gcagttgtca taattgattt
     6181 gacgattgta gcactttctt cagtaatgat ttgtttgcca ttccaaggag caattccttt
     6241 atttgccagc acagcagcta attttgctat gcctgtagcg gtgacattga gggagcattg
     6301 tctgaaataa gcatcgagta tctccggtac gtctccttcg atcatttgat tacctttcat
     6361 atagtaagca agagagcgat tgatatcacc tgttcttgat tcagaatgat agatttcttc
     6421 attcaatgtg atattaggat catcacagat ctctttcata aatgacaaga ttcgctcaaa
     6481 tttttcatct gcatctttac cggcaatcaa cgaggttgtt gcaatcgctc ctgcattaac
     6541 aaaaggattc attggatgtt tgcgatgatt gatttccata ttcatgatag aattgaaggc
     6601 aaaaccggtt ggttccgtgt tgattgtttg aaagactttt tctattccgt tgtctttgat
     6661 tgctaataac aatacgggaa cttttgacat gctttcgatg gcaaagcgca catcggcgtc
     6721 tcctccctca atcagttgtt ttgtatccaa atcataaatc gccataccta attgtttggg
     6781 atccacttca gctaatgctg gaatgtaatc cgccacttgt cctgtttgat aatagtggaa
     6841 attttttcca atcaattgtt ctaattcttt tacttccatt tgaataacca gctttcattt
     6901 tttatttttc tgcatgctgg aatttatgac ggcatgcgta gatcgtaaat ggaataacta
     6961 agacgataag ataagaaaca atcagaatcg tttcataaat cccatgctgt ccagctgcga
     7021 tactagcagg tggaacaaat gagatacaaa aagcaaaaag tgaagtcaaa agacctaaac
     7081 aagcaacgat tacttttaca acttttccgc ctggaacctg ataggctctt tcaagactgt
     7141 catctttaaa aaccagcttc aaatagcccg cgaaaaacat aaagtacaac atcaggtaga
     7201 ttacaacagt caatgacatc gctgcaagaa atgaaagatt attccccccg ccgcttaatg
     7261 taaagacggc tgcccagatt gttgcaacga tcccatttgc gatcatcaaa tgagcaggaa
     7321 caccgtgtgt gttttctttt gcaaaatatt caggaagtaa tccttcttct gctgctacgt
     7381 gaagtgcttt tgaaggagag gttacccaag aagcaatttc tccgatgaca cctaaacaaa
     7441 caagaatcgc aagaattttc gcaatccatg caagatttgg atcaatgtgt cttaatagga
     7501 attctaacga ttgaatcacc cctgtattaa gagacaaacc atgttgaggt actgtagtag
     7561 cgacggtcaa tccgccaaaa gtatctaaaa tgattgccag aataaccaat acaaacattg
     7621 ctaatggata atccttttta gggttttcca tttcatttgc atgactggca gatgtttcta
     7681 cgcccatata tgctaaaata aaggaaacaa aaacaaccag cgtaggcaat ttcgaaaaat
     7741 caggaatcaa tgttttcata gacagtatcg tttgtagtgg agcgcctgaa gcgacataat
     7801 ggatccctaa gccaaacaag actaatgctg gcaacaagat accagctaca aaaccgatcc
     7861 gagcaatcaa tgaagtgtat ttggttcctt ttaactgtga gagtaaaata gcccagaaaa
     7921 taatcaatag gattgccaat ttcagatatt tattgttttg tatagcacca atacccgttg
     7981 cataggataa tgcaccagtt aagaaataca gcatggtcaa gtagccaact gttatctcaa
     8041 accattggaa aaagattgcg gcaaaaccga atcgttttcc caatgtgcga ctgacccaag
     8101 tatagacacc gcctttttcc catcctttca ccgttgccat ctctgctgcg cacaatgcca
     8161 cagggataaa ccatagcaaa ccgcctaata acaagaaaaa tactaagctg aagcctgagg
     8221 tcgcgaatgt gggatattca tataatgaaa cgaccattga gatggtcata gccaaaaagc
     8281 caaagaacgt taattttttt gcaggaattt tcgttttgct ttgttgcatg aatcttcctc
     8341 ttttctattt gaatttgttt caattgagtt tgttgccaaa gaagaaaaag tccaatcctt
     8401 cttcttgttc ttcttttgta gtagtgatcg ttgtaaagtg catcgccgat tttaaaatca
     8461 ctgttgtcag cacataggca tacacggcag taatgatgac accaatcact tgaatcccta
     8521 agaagtacca gctgcttgga ttcgttagta aatggctgtt agatgcaaag atccctatca
     8581 gaatcgtacc tgtgaatccg cccatgccat gaacacccca cacatcaagt gcatcatccc
     8641 attgtttctt tttacgaaaa tcaacagcag catgacagac gatacccgca atgatcccaa
     8701 tcagcatggc actttttgct tctacataac cggcacaagg cgtaatggct gccaatccag
     8761 caacggatcc tgttagtacg tcgacaaaat caaagccatt tccttttact tttgcaatga
     8821 tcagccaagt aatcatcgca aatgcaagag cgatgaatgt tgaaccaaat gcattgactg
     8881 cttgttcatc tgctcgaagt gcaccaccgg aattaaaacc gaaccagcca aaccaaagca
     8941 gtcctgtacc gatagcagct gccataagac tgctatgctg acttctttct cctaaaggaa
     9001 tctttctttg accaagtgtg aggatacatg ctaaagagcc gaatccagct gtcgtgtgga
     9061 tgactgctcc accagcgaaa tctctaaatc ccatctgatc caaaaatccg ccaccccata
     9121 tccagtggca aacagggaaa taaatcagca gattccaaaa aataacaagt ataatatagc
     9181 cttttaaatt caatctccct gcaaaagctc ctgtcatcaa aggaacggta atcacgataa
     9241 acatcagttg gtaaagaaag aacattaaaa atggaatcgt tgcaccatga aacatgttgg
     9301 ggaagcttgt aacatgtctc atcaaaaaga aatctgccgg gtttccaata acaccgccga
     9361 tatcccgtcc aaaggccaat ccgaatccac caaataccca cataatcgtt gttatcccaa
     9421 cggagagaaa agattgagac atgatcgtta gtatattatt cttccgtgct aatccgccgt
     9481 aaaaaaaagc tagaccagga gtcatcaggc aaaccattgc tgtacataaa atcatgaatg
     9541 ctgtatctcc tgtattgata ggtaatgtat tcattttttt cgcctccttt ctacgattac
     9601 gagtataaaa gaaggtgtag ttgttttttt cttctgtttt tttgaatta
//
LOCUS       DANKHL010000107         9599 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000107 DANKHL010000000
VERSION     DANKHL010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.111"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(41..3715)
                     /gene="rpoC"
                     /locus_tag="PW101_001834"
     CDS             complement(41..3715)
                     /gene="rpoC"
                     /locus_tag="PW101_001834"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDL2514232.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="PW101_001835"
     CDS             complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="PW101_001835"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL2514233.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8118..9086
                     /locus_tag="PW101_001836"
     CDS             8118..9086
                     /locus_tag="PW101_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2514234.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9104..>9599
                     /locus_tag="PW101_001837"
     CDS             9104..>9599
                     /locus_tag="PW101_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2514235.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRA"
BASE COUNT         2584 a         2124 c         1911 g         2980 t
ORIGIN      
        1 gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca
       61 ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg
      121 ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga
      181 tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga
      241 tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat
      301 ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc
      361 ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac
      421 ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa
      481 ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta
      541 cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg
      601 gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt
      661 tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta
      721 cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg
      781 tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac
      841 cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag
      901 taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt
      961 cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac
     1021 atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga
     1081 tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta
     1141 cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc
     1201 cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga
     1261 ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca
     1321 aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg
     1381 atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg
     1441 ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag
     1501 aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga
     1561 tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga
     1621 tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga
     1681 tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg
     1741 tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt tcttttactt
     1801 tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata
     1861 ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt
     1921 ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag
     1981 gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc
     2041 attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat
     2101 gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt
     2161 cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc atatcttgag
     2221 aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca
     2281 acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc
     2341 catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt
     2401 gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca
     2461 gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac
     2521 gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac
     2581 gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca
     2641 tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta
     2701 gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg
     2761 ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta
     2821 ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta
     2881 acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac
     2941 ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag
     3001 ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa
     3061 tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt
     3121 tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg
     3181 cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt
     3241 ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag
     3301 caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg
     3361 aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca
     3421 tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc
     3481 ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa
     3541 aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct
     3601 caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta
     3661 tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat
     3721 ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta
     3781 aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt
     3841 cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat
     3901 caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt
     3961 cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga
     4021 atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt
     4081 tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg
     4141 caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag
     4201 ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca
     4261 atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc
     4321 catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac
     4381 cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg
     4441 ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga
     4501 tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag
     4561 gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac
     4621 gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta
     4681 acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt
     4741 gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt
     4801 tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg
     4861 gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac
     4921 ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt
     4981 cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag
     5041 tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc
     5101 cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca
     5161 tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg
     5221 gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat
     5281 ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac
     5341 catcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag
     5401 taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac
     5461 ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg
     5521 caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt
     5581 tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg
     5641 ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt
     5701 ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga
     5761 atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag
     5821 gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac
     5881 cggcgcggtc acgagtcaat ccgccaggtc ctaaggcgaa aagacgacgt ttgtgtgtca
     5941 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
     6001 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
     6061 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
     6121 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
     6181 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
     6241 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
     6301 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
     6361 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
     6421 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
     6481 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
     6541 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
     6601 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
     6661 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
     6721 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
     6781 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
     6841 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
     6901 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
     6961 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
     7021 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
     7081 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
     7141 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
     7201 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
     7261 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
     7321 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
     7381 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
     7441 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
     7501 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
     7561 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
     7621 agtttttgca acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt
     7681 tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga
     7741 caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga
     7801 ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac
     7861 tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt
     7921 tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct
     7981 aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta
     8041 ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa
     8101 ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac
     8161 tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa
     8221 caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca
     8281 aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa
     8341 atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc
     8401 ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca
     8461 ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca
     8521 atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa
     8581 tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg
     8641 gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg
     8701 tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc
     8761 tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa
     8821 gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc
     8881 aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggcaaacaaa
     8941 cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa
     9001 cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc
     9061 cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc
     9121 tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata
     9181 cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg
     9241 tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct
     9301 aggcttttta gggagtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa
     9361 aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg
     9421 tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga
     9481 gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat
     9541 cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaa
//
LOCUS       DANKHL010000108         9570 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000108 DANKHL010000000
VERSION     DANKHL010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.112"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            147..1016
                     /locus_tag="PW101_001838"
     CDS             147..1016
                     /locus_tag="PW101_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HDL2514236.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="PW101_001839"
     CDS             1225..2250
                     /locus_tag="PW101_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL2514237.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="PW101_001840"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="PW101_001840"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDL2514238.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="PW101_001841"
     CDS             3333..5036
                     /locus_tag="PW101_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2514239.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="PW101_001842"
     CDS             5038..7071
                     /locus_tag="PW101_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2514240.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="PW101_001843"
     CDS             7082..7522
                     /locus_tag="PW101_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2514241.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="PW101_001844"
     CDS             complement(7722..8849)
                     /locus_tag="PW101_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2514242.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="PW101_001845"
     CDS             complement(8852..9529)
                     /locus_tag="PW101_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2514243.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
BASE COUNT         3257 a         1652 c         1954 g         2707 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg
//
LOCUS       DANKHL010000109         9552 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000109 DANKHL010000000
VERSION     DANKHL010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.113"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(13..996)
                     /locus_tag="PW101_001846"
     CDS             complement(13..996)
                     /locus_tag="PW101_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HDL2514244.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1001..1648)
                     /locus_tag="PW101_001847"
     CDS             complement(1001..1648)
                     /locus_tag="PW101_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2514245.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1733..2365)
                     /locus_tag="PW101_001848"
     CDS             complement(1733..2365)
                     /locus_tag="PW101_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2514246.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2381..2932)
                     /gene="coaC"
                     /locus_tag="PW101_001849"
     CDS             complement(2381..2932)
                     /gene="coaC"
                     /locus_tag="PW101_001849"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HDL2514247.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(2929..3666)
                     /locus_tag="PW101_001850"
     CDS             complement(2929..3666)
                     /locus_tag="PW101_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HDL2514248.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3663..4652)
                     /locus_tag="PW101_001851"
     CDS             complement(3663..4652)
                     /locus_tag="PW101_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL2514249.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            4910..5452
                     /locus_tag="PW101_001852"
     CDS             4910..5452
                     /locus_tag="PW101_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2514250.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            5469..6554
                     /locus_tag="PW101_001853"
     CDS             5469..6554
                     /locus_tag="PW101_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2514251.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            6566..7372
                     /locus_tag="PW101_001854"
     CDS             6566..7372
                     /locus_tag="PW101_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2514252.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            7373..8200
                     /locus_tag="PW101_001855"
     CDS             7373..8200
                     /locus_tag="PW101_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2514253.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            8197..9270
                     /locus_tag="PW101_001856"
     CDS             8197..9270
                     /locus_tag="PW101_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2514254.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         2878 a         1764 c         1957 g         2953 t
ORIGIN      
        1 atcagcatga ttttattcag ttatcttcaa aatgattttt ccgatgtttt tgtttgcttc
       61 catatattca tgcgcttgtt tgactgcgct gaattggaat gtacggtcaa tgattggccg
      121 aatcttgcca ttttctagaa aaggcccagc tttttgcata aaagtttgtg cgagctctgc
      181 tttatactga tcacttctag gtgtcaataa tgtaccaagc agttggacac gtttcccaat
      241 caatacacca aggtctatct caggcacgat ggtcccacct agcataccaa tcaagaccca
      301 acgtccatca acggctatag aagctaaatt cttttcccag taagaagcgc cgatgaaatc
      361 caaaataaca tcgactcccc gaccttcagt gatttttcgt acctcttctt gaaagtcttg
      421 ttttttatag ttgatcaatt catctgcacc aagttcttta caaaaggcga gtttttcttc
      481 tgatcctgca gtaacgatga ttttcgcgtg agaaagctgt ctagctaatt ggatcgctgc
      541 tgttcccaca ccacttgctc ctgcatggat caagacagtt tcgccttctt gtaattttcc
      601 aagccagtaa agtgtttgat aagcagtcaa aaaaacttct gagattccag cagcagaaac
      661 atagtctagc gtatcaggga gaaggattgc acgatcggca ggcatcacag catattcggc
      721 ataaccacct agattgacta atccgtatac ttttgtccca ggagtaaaag tggagatatc
      781 acttttattc tcaactacct ctccagctac ttcgactccc aaaatagggt agggaggttt
      841 caaagagact gattctcttt tcataatatc tgttcgattg acagctgctg tatggacttt
      901 gatcagtaat tcgccttgtt ttggtttagg aacaggcact tcaatttgtt ctaatacatc
      961 tacagaacca ggatgcttga ttgaaatggc acgcatggat ctattcctcc tttgcccatt
     1021 cttggaatgc tgtattcaac tctttgctac cttcagaaag tactagagtc gtacgagaaa
     1081 tatccgtttc ttccactaga gcggcaagta cagcagcaac tgtagagcga gtgatcgttt
     1141 ttgatgcctt ctcattaggc ttagttaatt gaatggaagt aacttcttca tcgttagtca
     1201 atgtgactgg ttgaagaatg acaaaatcta agttagttcg tttcagccat tcatctgcat
     1261 aatgtttagt aatataataa tctgtcatgg actcaggcca ttttgctggt tcatctgcat
     1321 aaacagcact gaccatcaga tagcgagaga catgcgcatt ttcagctgcg atcactgttt
     1381 tgattgcgcc atccaagtct acttgcagaa gattctttcc ttgagagccg gcagcaaaaa
     1441 tcaatacatc gatgcctttg aatgcttcgg tcattttttc cacggaccaa gttaaatcaa
     1501 aagagacata cgaaacattc tcttttttta ctaatgtttg ggaagcaaca tctctcaccc
     1561 ctgcaaaaat ttcatgggaa cttggagcta aatcttttat caggtgtcgg ccaatttgtc
     1621 cgtttgcacc tacaacaaaa attttcattt ggatcatcct ttcgacgtca aatctttcca
     1681 ttcaatagta acaccaaaag agaaaaaaat cagagatcgg ctacgatgac tatcagttag
     1741 taagagaagg gtgcttgaag tatcttaaca agacattgca tactgctgca gtcgcaagac
     1801 ctgcgacgat tccttcaggt ataccacttg taaaaataac cgctagaata gctgaataaa
     1861 cagcatttaa atttgttccg atgactttgg cataatcttg ctggaataaa aagtagatca
     1921 tattcatcac taaaatcgta ttggtcaatg aaccggaaag tccagctaag aataaagaaa
     1981 cggaatctgc tttttgtttc attagtttgc gcagaccttt ataaacgaaa taaggaacca
     2041 ccccaatcaa gatgcgagga ataaacgcga tcagcaaggc tttccagctt ccatgttctg
     2101 ttccgatgac tgggataaac ggagaaaaga caaaagacaa aggtgtttgg ataactgtgc
     2161 tacggatcat gctgatcagt ccaaatgtac cgccaagaaa agcacctagt cttggtccta
     2221 aaacgatcga agcaatgatt acaggaatat gcatggttgt tgcattgatt ggaccgatag
     2281 ggataaaacc taaaggcgta acagctaata aaatcaaaat agctaaaaac atcgcagtta
     2341 atgtaaagtt ttttgtattt ttcataagta ctcctttttt ctaagcattt ctttcattaa
     2401 gagtcagcat gacctgttcg atgatcgtct gaatatcagc taaggcacct tttccaaaat
     2461 ctccacaagc taaaagagat tctcttggtt caatttcctg atagccgaca gatctcaatg
     2521 tttccagatt acgctgagtg atcgggtttt gatacatatt cgtgttcatg gcaggggcaa
     2581 tcaattttgg tgttcccact ggaagcgcca ttgcgactgt tgaaagaaga tcgtctgcaa
     2641 tgccgtttgc tagcttgccg ataatgtttg ctgtagcagg cgcgatgaga aacaagtcag
     2701 cttgttttgc tagttcgata tgattgatta cctgcggatt tgattcttgc atgacgtctg
     2761 tatggactgc gcgcttcgac agagattgca aggttaatgg cgtgataaaa tgtgtgctgc
     2821 ttttagacat aatcacatct acctgggcac ctaatttgac gaattgactt gtaagatcag
     2881 cgcttttgta agcagaaata cttccagtaa cgcctagtaa gatctttttc attttgattc
     2941 ctccagtcta attttctcag taatcaatgc agcaatctca gatttacttt gagcttcttc
     3001 cactgttcca tctttagata agagatagcc gtggtgtgtc tcgccatgga tttccgtcaa
     3061 atcatttgcg aaaataaaat cagttttgtt atttatcaga ctattttttg cgactgccag
     3121 aagagattcc tttggtacat ctactaatag tttgaaacca atgatttttg cttttggctg
     3181 ccattcacgt agataagaaa tgatttttgg tgttttcttc aaaacgagca caagatgctc
     3241 ggtgttagaa gagatctttg aaacagtggt atctgtctgt tcattttccg caaaccaatt
     3301 gtccagttct tcttgcgttg aagaagtggg taggtttttt gccagctgtt cttctgaaaa
     3361 actaaaagca ggagtaaaat cactgactgc catactgtgg ataatcgcat cgtagtgttc
     3421 ttttttcgtt agttcttcta attgtaaaaa taagtccagt gcagattcga tcgtataaat
     3481 cgttatattg gagtgtcttt tcggtttcag tgcttgtttc gtagtcacgt aatcgattgt
     3541 ggtatccgga ttggcagcta aataatcagc taatgcttgc cctaatcgtc ctgtcgaatg
     3601 atttgtaata gcacgtactt gatcgatttt ttcagtcgtt cctccagctg tgattagaat
     3661 cttcatttat ggtcacctac atttactagt gttcttcctt ggtgtgttcc agcaagtagt
     3721 ttagatgctg tttcaggcaa ttgatccaga gtaatctctt ggacgaccag ttcatctgca
     3781 atctgcaaat ccgccatctg ttgccataaa gaaagacgtt gatctattgg tacattgaca
     3841 gaatcaattc caatcaactg gatgtttcgc agaataaaag gcaagacagt cgcgtttagt
     3901 ttgattcccc cagcattgcc acataaagct actgcaccgt tataagagat caatggaaga
     3961 agactgctta gctgctcgcc acctactgtg tcgatgacat agtcgatttg ctgttttcct
     4021 aaagcttttg ttgtctctgg cagaaattca tcaggtcgta cgacttgcaa agcacctaga
     4081 ctttttagcc atgtgacaga ctcttctttt ctggaaaagg taatgattga ctggaatcct
     4141 agtttatgta acagggctat cgctgtactg cctacaccgc cagaagcacc agtaacgaca
     4201 acttttttat cggaggtcat accttgacgg attaaagcgt tcacacacaa catagcagta
     4261 aaccctgctg taccaaggat catcgcttga cgcagcgtca tattttttgg tagaggaact
     4321 aaccagtctc ctggtacttt ttgatattgg ctataaccgc ctggatgggt gacgccaagc
     4381 ccaaaaccag tagcaagtac aagatcttct tttgaaaaac gtggattgtt actttggacg
     4441 acagttccgg ataaatcaat ccctggagtc atcggatatg ttcgtatcac ctgaccagac
     4501 tcgctcattg ccaatgcatc tttataattg acatcagaat aggcaatttt taccaagact
     4561 tcattttcag ccaactcagt aaatggcaga tcactgattg ctgtatggaa aacaggttcc
     4621 tttattacct gaaaagatcg aaaagcgttc attcgtgaga cactcctttt aaaattttct
     4681 tttatcatac cataaagcct actcttttat tcttgttctt ccgtaaattt agacaaaaat
     4741 taggtgaaaa ataaagaatc aaacaactag aatttattcc aaaagataaa gaaatattca
     4801 gcaaaaaaaa ttttacaaag ggttgccttt ttcaataaat ttcgtatact aatacgagtg
     4861 gtagtaaaca ggaagtttac tattagaaaa cttcgattcg gaggaaatta tggaaattgg
     4921 tgagaagtta agaaatttgc gcatccaaaa gaatttgaca caagaagaac taggcgaacg
     4981 cacagatttg tcaaaagggt atatttccca gttggaacga gatttaagtt caccatcaat
     5041 ggaaacattc ttttctattc ttgaagtctt aggtgtgact ccagaagaat tttttcatca
     5101 agaagcagcg aatctgcaag tggtttattc aaaagaagat catacggttt atttcgatga
     5161 agaaaacggg tatgaattgg aatggctcgt ggctgattcg aatgaaaaag aaatggagcc
     5221 tgtcttgctg acttttgaag gtgcaggaga atataagacg tttgaacctt cattatctga
     5281 aacgtttatt tttgtattag aaggggaatt agagttaagc ttaggagaga cggtctatgt
     5341 ggcaaaagca ggacagtcga tctattatca agcgacaaaa caacatcaat taaggaatca
     5401 tctcaaaaaa cgaacaaaag tattgattgt tgcaacgcaa tcatatttgt aatatagggg
     5461 gtaaaaacgt ggggaaacca atcatttcat ttgatcaagt agtgaaacgc tatgatgatg
     5521 aaacagtatt aaaaaaagta agttttgaga tcgaacaagg aaaattttat acactgcttg
     5581 gtccttccgg ctgtggtaaa acaacgatcc tacgtattat agctggattc accgaagcta
     5641 cagaagggga catctatttt gaagggaaac ggttgaatga tttgccagct aataagcgac
     5701 aagtaaatac agtcttccaa gattatgcac tttttcctca tatgaatgtt tttgataacg
     5761 ttgcttttgg attgaagatc aagaaaatgt ccaaatcaga tatcgaaaag aaggtaaaag
     5821 atgctttgcg tatggtccag cttcctggat acgaaaaacg ggagattagt gaaatgtctg
     5881 gtggtcaaag acagcgtgta gcgattgcac gagcaattgt aaatgaaccg aaagtattgc
     5941 tattagatga accgttatcg gcgcttgatt tgaaactacg tacagatatg cagtatgaac
     6001 tgcgggatct gcaacaacga ttaggaatca cgtttatttt tgttactcat gaccaagaag
     6061 aagcgttggc aatgagtgat gagatttttg tcatgaataa agggaaaatc gtccaaagtg
     6121 gtacacccgt agatatttat gatgagccaa tcaatcattt cgttgctgat tttgttgggg
     6181 agagcaatat cgtggacggt gtaatgatcg aagataatct agttgagttt gttggtaagc
     6241 aatttgaatg tgtcgatggc gggatgcgtc taaatgaacc agttgaagtt gttttacgcc
     6301 cagaggattt gacgatcacg acaccagata aaggaaaact agtcgtaacg gtcgatactc
     6361 agctgtttcg tggtgtccat tatgaaatca tctgttatga tgaacagcaa aatgaatgga
     6421 tggtccattc aacgaaaaaa gctaaagaag gaagcaaagt aggattggcc tttgagccag
     6481 aagatatcca tgtcatgcgt ttcaacgagt ctgaagaaga attcgatgca cgtcttgaca
     6541 gctacgaaga atagggggga caagaatgaa aacaatgcgc aggatctatt ccgtccctta
     6601 tattttgtgg ctcggtttat tcgtgatcgc gcctgtatta atgatcattt atcaatcttt
     6661 ttttgatatg aatgggcaat tcacgctgaa taattatcta tcttatttta cgtcggggac
     6721 atcgctttct atgacgatca attctgtatg gtatgcattt ttgatcacgt ttttcacgct
     6781 gatcatcagt tacccgactg cttatttttt aacaaaactg aagcacaagc aattatggct
     6841 gatgctgatt attttgccta catgggtgaa tctgctgctt aaagcttacg catttattgg
     6901 gattttcagt gtgcacggca gcgtgaatca tttctttgat tttatcggga tcgggccgaa
     6961 acagatatta ttcacagact tcagttttct gttcgtggca acgtatatcg agattccatt
     7021 tatgattatg ccaattttca atgcattgga agagatgaat ccttctttga tcagtgctag
     7081 tagagattta ggcgcgaata gctttgagac cttccgacga gtgatttttc cgttatcatt
     7141 aaatggtgtg aaaagtggtg tccaagcagt atttattcct tctctttctt tattcatgct
     7201 gactcgtctg attggcggaa atcgggtgat cactttagga acggctatcg aacagcattt
     7261 cttggttaca caaaactggg ggatgggttc tacaatcggt gtgattttaa tcattgctat
     7321 gttcatcgtg atgctcctga caggcgagaa gaaaaaagga ggtcgctcat aaatgaaaaa
     7381 atttaaatgg tcgtatcttt atttagggat cgtcttttta ttcttatatg caccgatctt
     7441 ctatctgatt ttttattcct tcaatgaagg aggaacgatg aacagcttta caggatttac
     7501 ttgggaaaat tatgaagctg tttttgaaga tacacgtttg atcattatcg tattgaatac
     7561 gttcatgctg gctttcttat ctgcattgat cgccacgatc atcgggacat ttggggctat
     7621 gggaatctat tacacgaaaa aacgacgtgc acggacagcg ttgttgagca tgaataacat
     7681 cttattagtc tctcctgatg ttattatcgg tgctagtttc ttgattttct ttacactact
     7741 aggattcgga ttaggttttc agagtgtatt gctttcccat attgctttca gcattccaat
     7801 cgtcgtcttg atggtcttgc caaaattgca ggaaatgaac gattcaatgg tcgatgcggc
     7861 tagagacctt ggtgctaata atttccaagt agtcaaaaat atcattttgc cttatctttc
     7921 tccaggaatc atagctggat attttatggc atttacctat tctcttgatg attttgcggt
     7981 tacatttttc gtcacaggaa atggctttac gacgctctca gtagagatct attctcgtgc
     8041 aagacaagga atcagtttag aaatcaatgc gctaagtgcg ttagttttct tgttctccat
     8101 gatattggtc attggatact actttattag taaagaaaac cgtccaaaac ggctgaagaa
     8161 aaacagaaga gtacgcgagg gggtggcacg gctgcgatga aaaaattaca atctctgttt
     8221 ttaggtattc tcatcattat tgcggtgctg ctgcttggtg ttcgtcaact tgaaaaaaca
     8281 agtggcatga gtggagcaaa ggtacttacc atctataact ggggagatta tatcgatcct
     8341 agtttgatca cgaaatttga aaaagaatat ggatacaaag tgaattatga aacatttgat
     8401 tctaatgaag cgatgttcac aaaaatccag caaggtggga caaactacga catcgctata
     8461 ccaagtgaat atatgatcca aaaaatgatc aaagaaaaac tagtacttcc attagatcat
     8521 tcgaagatca aaggattaaa taatattgat ccacgatttt tagacttgga ttttgatcca
     8581 gataatcagt attcgattcc atatttttgg ggaactttag ggatcgtata taacgataaa
     8641 ttctttgatg caaatcaaat taagcattgg gatgatttgt ggaagccgga attaaaagac
     8701 agtttgatgc tgattgacgg tgcacgagaa gttatggggc tttctttgaa tagtttaggc
     8761 tattctttga atagtaagaa tatggcacag ctgacagaag cagcaaataa attgaatcat
     8821 ttaacaccaa atgtcaaagc aatcgtggca gatgagatca aaatgtatat gatcaatgaa
     8881 gaagcctccg tggcagtgac attttctggc gaagctgccg atatgatgtg ggaaaatgaa
     8941 catttacatt atgtgatccc accagaagga tccaatcttt ggtttgataa tatcgttatg
     9001 ccaaaaaccg tgaaaaataa agaaggtgcc tatgatttca tcaactttat gctagaaccg
     9061 gaaaatgcgg cacaaaatgc agagtatatt ggttattcta caccaaatga agaagcaaaa
     9121 gcgttattgc caaaagacat ttcgtcggac gaacaattct atccatctga tgacacgatc
     9181 tcccatcttg aagtctacaa agatctagga tcgaaatacc taggcatcta taatgaccta
     9241 ttcttagaat tcaagatgta taggagataa tggcaaaact cagtaagaat ggggacttat
     9301 caacgttcta aagagctaaa aatcgtcctt tttctatctg agttttccaa tcttctacca
     9361 aaagcggatg aaacgccgct ttttttgtgt tattttattt tttctaccac gtttttctac
     9421 cagagaaaag tggacaaaac cattgtaaaa tagggcccga gacgggctag taaatctaag
     9481 attgaaaaag ttacagaagg ataaagtaat tggaaactat gcaaatatac ttaacccttc
     9541 tgtaatctat ta
//
LOCUS       DANKHL010000110         9551 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000110 DANKHL010000000
VERSION     DANKHL010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.114"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            1..417
                     /locus_tag="PW101_001857"
                     /pseudo
     CDS             1..417
                     /locus_tag="PW101_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(325..1041)
                     /locus_tag="PW101_001858"
     CDS             complement(325..1041)
                     /locus_tag="PW101_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDL2514255.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(1091..1300)
                     /locus_tag="PW101_001859"
     CDS             complement(1091..1300)
                     /locus_tag="PW101_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514256.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            1577..2850
                     /locus_tag="PW101_001860"
                     /pseudo
     CDS             1577..2850
                     /locus_tag="PW101_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2957..3988)
                     /locus_tag="PW101_001861"
     CDS             complement(2957..3988)
                     /locus_tag="PW101_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2514257.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(3995..4825)
                     /locus_tag="PW101_001862"
     CDS             complement(3995..4825)
                     /locus_tag="PW101_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2514258.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4822..5637)
                     /locus_tag="PW101_001863"
     CDS             complement(4822..5637)
                     /locus_tag="PW101_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2514259.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5634..6458)
                     /locus_tag="PW101_001864"
     CDS             complement(5634..6458)
                     /locus_tag="PW101_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HDL2514260.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6448..7548)
                     /locus_tag="PW101_001865"
     CDS             complement(6448..7548)
                     /locus_tag="PW101_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2514261.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7726..8511
                     /locus_tag="PW101_001866"
     CDS             7726..8511
                     /locus_tag="PW101_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HDL2514262.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8544..8927
                     /locus_tag="PW101_001867"
     CDS             8544..8927
                     /locus_tag="PW101_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDL2514263.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            9003..9134
                     /locus_tag="PW101_001868"
     CDS             9003..9134
                     /locus_tag="PW101_001868"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2514264.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9103..9339
                     /locus_tag="PW101_001869"
     CDS             9103..9339
                     /locus_tag="PW101_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2514265.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            9351..9518
                     /locus_tag="PW101_001870"
     CDS             9351..9518
                     /locus_tag="PW101_001870"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2514266.1"
                     /translation="MELGGLPELDGRYTVFGQVYQGLEIVAAIAAQPTNSEDVPIEPV
                     FIRQINFEKTS"
BASE COUNT         2796 a         2267 c         1850 g         2638 t
ORIGIN      
        1 gttccatgag ttttatttgt gttgatggga caacccatca gctatttacg atccttgaag
       61 atcgcagact ttacaaacta actcaatact tcctgcactt tcctagaaaa gcccgtttaa
      121 aggtcaagta cctagaatgc cagctattgt cagctgctta aaacggtctt tccaaatgcg
      181 gagatcgtaa ctgatcgctt tcatattgtc caacatatca atcgcagctt caatcaattg
      241 cgtatccaga tcatgaatcg ttttcgagcc tcgcactctg aaaatcagaa gaagtaccgt
      301 cggctcaagc gatattggaa actcttactc aaagattcca cgacattgga gccgctcaaa
      361 cggcattatc atcgactgtt taaacgacca attagtcaaa cagaaatcgt cgatgaaccg
      421 ttgtgcacaa tggtgtcctg aacgtacagc gacaccttcc atatccagag cagtcgccac
      481 atcacgggga tggatgccat ccaaattaaa agcaaccacg ccagtatgat gttcaggatc
      541 ttgcggtccg taaatggtga taccgccgat cgcttgtaat ttaggtaaaa cgtaggcaac
      601 cagttcttgc tcataacgat gaatattctc cataccaatc tcattcaagt aatcgattgc
      661 tcctgcaatg ttcggagtac cagcttcaaa tttccatggc agctctttcc atgaactatc
      721 gtaaagattg acaaaatcta tcatttcacc gccgaattcg actggctcca tttgttctaa
      781 caattgacgt ttgccatata atacaccgat tcctgtcggc ccgcacattt tatgcccgct
      841 aaaggcgaaa aaatctgcat ccaacgcttg tacatcaact gtcatatgcg gtgctgcttg
      901 ggcaccgact ggactatcct caactacaac agcctgttca accctcagcc gtttcaagca
      961 caacaaataa ccttctggat ctggtttgcc gacctgaaga tcatcgatcg tcacaaagga
     1021 atcaattttt tccttaatca tcggatgtgc ttcaaaaaaa tagtctagct cccgcagttc
     1081 agaattgaga ctactccgat tttccagtgc tgctgatccg ctgctcaaaa tgttcagcat
     1141 ccactggtct cccaaccgct tgacttatcc cttcataaaa ggacagttct gctgccgtcc
     1201 caacaccttg ttgatagtct gcaaaaggaa ttttatagcc gaactgctcc agtaaatagg
     1261 cttcagtaaa tagtgatgaa tactctcgta aaacatccat tcactgtcaa tcatcgtccc
     1321 atcaaaatca aaaataagtg ctttggtcat cttctttccc ccttacccaa aacaagcaaa
     1381 agctgtgtcc tgctctctct acagtatcag cataaaaact gccgtctgaa aagtcaaagg
     1441 tgaaagaaat gtggcgctat aatttctagg actgatagta aatcattcta tcagtccttt
     1501 tgagcataaa aaaacaaaac atacagaagc tccgttagaa ttgaatcacc acaacacaat
     1561 caaaggagcg ttctgtatgc ctgatcttca tcttatcaaa ctattactag gaatcaaaga
     1621 tggaaacatt caaatcactc atgtcgagaa gaaaaaaata cattcaatca acagtatcgt
     1681 tgtttctgcc acgctcatta aaacgattca gccctgtcct cagtgtaaac aggtgaatca
     1741 acatgggctg atcgttaaga acggaaagaa aaaatcgctg gtccttctca ataagtgtgc
     1801 gaatcaacgg acttatcttg ctttggcaaa gcaacggtac ctctgtcgtg gctgtcacac
     1861 ctattttacg gcgaatacct acattgttga aaaaaactgt tttatcgcca aacaagtaca
     1921 ttataaaata ttggaggaat tgactgaaaa acaagcaatg acgacgattt ctaaacattg
     1981 cggcgtttct tggtcaaccg tttcgcgaac actcgcttct ctgctcccca tgacaaacgt
     2041 gaaaataaat tggttgcctc gctgttcatt ggtagatgaa tttcgttcat tgaaaaatca
     2101 agtcggtccc tattcttttt cttgtatgga tggcgatact gggaaattac tggatattct
     2161 tccttcacgt aagaagaaag atctcatttc ttattttatg caatttgaac gacgtgcacg
     2221 tttaaacgta aaaatactcg taacagatat gaacgcctct tatgctttgc tgataaaaga
     2281 gtgctttcct catgccaaac tcgttgtgga tcgctttcac atcgtcaagc acctcattcg
     2341 cagttttaag gatatttgac tatgagtcat gaaaagcttc gaccgaaacg atccaataca
     2401 agccaaacac tatcgacaag tgaaggcttt atctcgactt ctgatcaaac gacaggacac
     2461 gcttgtttat gataaatgga caaaatggcg taattttggt tgggcctatt taaccgaaag
     2521 tgaagtcgta gaacgtctac tttctacttc tgatgaacta cgaatagctt atgcttacta
     2581 tcaagaaatt cttcaagctt tttatgacaa agaagcggac atcttctttc agcttgtaaa
     2641 aacaatgccc aagtcggttc caagagaact gcatcatata aaaaaagcgt ttataaacta
     2701 tgaatccggc attcgtttag ccttagagtt gccttactca aatggcaaaa tagaaaatct
     2761 gcatacccac atcaaagcat tgaaacgaat agcttacggg ttaggatgag aaccagaatt
     2821 ttcttaatca atcatctgat cacttattaa acataaaaaa acatctaaag cagaaaatca
     2881 attctacctt agatgttctt atatcactct atcagtccta tttgacaaag agccagaaat
     2941 gtttgccgac tatacactag ctttgttgtg caaaatgctg agtatcaatt tccacaggga
     3001 ttgctagaat atctttcagc tgaatgtata aggcatcgct aattttctct tcccgatcgt
     3061 tcaaccgatc cactaaaatt tctttgccct ccagcgtttg aaaaacatac cgttgaatgt
     3121 tgcctaaaat aaatgattcc ttccatagaa ccggaatctt aaccccctga ggaaccggtt
     3181 caaattgaat cagctccggt cttaaataat aaatgcagcg attgccctta gcggaaaact
     3241 cgtgggtata ctgattcagc tcataccatt cgaaacgatt atagttgccg ataaattccg
     3301 caacaaatcg gctctgagga ttctgataca attccttcga ggtggaaacc tgagccaccc
     3361 gcccagcttc catcacaaaa atccgatcac tgattcgcat cgcctcttct tggtcatgag
     3421 tcacaaaaat catcgtcatc cctagcgatt gctggatagc gcgcaaatcc cgctgcagct
     3481 gttttctaat tcgggcatcc aaagcactta atggctcatc cagcagcagg actttcggtt
     3541 cagtgaccaa tgagcgagcc agtgccaccc gttgtcgttg accgccagaa agaaactgga
     3601 catttttatc tgcctgctcc gtcatatcca ccaatgatag catctcaaaa accttttcct
     3661 gaatcacctc aggactcagc ttctttacct tcaaaccaaa ggctacattt tcaaaaacag
     3721 tcatagttgg aaacaatgca tactgttgaa aaacaaagcc gatattgcga tccttcgtag
     3781 gcttatcggc aatattctca ttgtccagca caatctcacc gctctttggt tgttccaatc
     3841 ccgtgataca gcgcagcaac gtagactttc cgcacccgct gggacctagt aaagtaacca
     3901 gctcaccctt ttcaatctga atttcatcaa tagctaaaat ctgtttttca tcaaaaaaca
     3961 gattgagatc atgtatttct aaaaacattt tccgtcacaa ctcctttaag ggcttctttt
     4021 tcgactgctt ctcttccctc gtctgcttaa gggacagctg ttgcttttgt cgataagtca
     4081 gcataaaggt agttaaagca attcccacca taaacagcaa atacaaaacc atcacagcgc
     4141 tagccaaatg gccgttttcg ttcatccgcc gcagcatata gatgcgtacc gtctgaaatc
     4201 gaccgccaat caacaggttc gtcagcatgt actccccaaa aaccgaagaa aaaatcatca
     4261 gagaaactag ggtcacaccg atacggatgt ttggaaacag cacctggata ataatcgtag
     4321 acatcggggc gcccaaggtt tctgctgcat ccactaattc cttcagatta attaagcgca
     4381 tctggttgtt aagactaaga tagacaatcg gcaacgccgt aataaacaat gcacctacca
     4441 acactacaaa cattgggata ccagtttcag catacgttcg aagcaaagcc gttaccaaaa
     4501 tcactcctgg taaagcatac ggcagcagga gcagcttttc cagccaacga ttcaaacgag
     4561 ggaaatacaa atgaatccaa ataatggtgg gaatcatcaa caacagaatc gtcaccaaga
     4621 ccacacctgc taataaaagt gagcgaccca ccgcagcaat aaattccctg tccgtcagca
     4681 gctgttggaa ccattgcaag gtaaaatcac taggtaaaag cgattttgac catttagtgg
     4741 agctggcata caaaatcgtt ccaataatcg gcagcaagta atacaggaca aagcagaaca
     4801 aaatcggttt tattaacttt ttcacgtctg caccaccttg cctttctttt gaataatcaa
     4861 cattgccgtt accagcagga cacctaaagc gactgccaac gcactgccta aattaggctt
     4921 ggcgaacaca tctcccgaca ctaaagccgc aattcgaatc gttaaaagat ttacattact
     4981 gctggtcagg gcatacgctg tttcataggt ccccatgcca ttagcaaata agatgacaaa
     5041 ggtgctggca atcgatggtc gcaaaaaagg caaagccacc ttgaaccaag catacgctga
     5101 tgaagcgccc agcaattgtg cggactccag ccattccttt ttcacttcct tcatgctggg
     5161 atacaaaaac aagaccccta acggaatttg gaaataggta tacgtgatgg tcaatcccca
     5221 ccaagaatac aaattgaagt ccgctagaaa tggcaaaatc attttcagca cacctacatt
     5281 ccccaacaaa ataataaaag agaatgccag cggaatccca gcaaaattag ctgccaaatt
     5341 ggaaacaaag gtgattttct cctgcaattt agtcgataga ttcattaagc ataaacataa
     5401 aataaacgtc ccgattaacc caatcacact ggagctgaca gcaatcagta cgctgttaac
     5461 aaaggcctgt gtgtaatatt tgttgctgaa aatatcactg taattggcca atgtccactg
     5521 gttagttaaa tcactgtgca agctgcacag aaccataaaa agcagcggca gcagtaaaaa
     5581 tgcacagaag ataaaggagg caggaaccag aaccaagcgt tttttcatct ctttcatccc
     5641 ctcatcaatt cttgaatgtc ctgcattttc tctgacggtg caattcctaa taaccagcaa
     5701 aacaatggag ccgccagcag ctgtggtaca gtttgttcct tcagcttttg gcgtttattt
     5761 gaaatcaaat aaaaaggaac ttcacgatgt gcagccaagc tgccgccatg cagtccgtgc
     5821 gcatccatcc cgtgatccgc agtcaccacg acttgataac ctttttgcag ccattcagga
     5881 aggtaaatag ccaataatct gtccgcctga ttaaccgctg ctgcatattc atgagaatcg
     5941 gcggtaaatt tgtgaccgac atcatcaata ttcatcgaat gtacgaccaa aaagtctggc
     6001 tgatactgat tcactaaata atgggcatcc gcaaataaat gcgaatcagg gtaagtgtcc
     6061 tcactataaa agatgccatg ctgaatcagt cgctcctcct gatgctggat gcgatcaacc
     6121 aaacgattat agggggcttg attgtacaac tcgctgtacc agtggtaagc ggcggctcct
     6181 gtttttccgc cagcttcctt aactaattca aacagcgacc actctttgga tcgctgagca
     6241 tggtaattac tatagatgcc attttcatag gctggaacgc cggtcatcag tacttcgtat
     6301 aacggacgtg aattgctggg cagctcactt tttaccttca ataagcttgc ttgctggtgc
     6361 tcaactaaat gattcatgaa cccaagctgg gcgacggctg tgtcgtagcg acagccgtct
     6421 agtataacta caatcagttt gtcaagtcta ctttgcatag gtcagtacct ccgcctgcca
     6481 aagctctgga agctctacag aggtcttgtt ccaagcttca ttgtcttcaa ctggcaccgc
     6541 cgcttcataa gcatcctccg gcagtaattt ttcagctact tctttaggca gctctacctt
     6601 gcgaataggt cgcgcatagc cctttgccaa attgatttgc ccttcatcag ataaaatata
     6661 ttcccgtgct agctttgccg cattgggatg aggggcattt ttattgatta aggttgtata
     6721 cccgctaatg atggagccgt ctgttggcac agttacctca aaacgctcgg agtcaatttg
     6781 gtcacgataa tttagtccat tgaagtccca aacaactgcc acatcgattt cacctttttc
     6841 aagatttgct acgctggcat caacggtgga caaacggcct ttttctgcca tttttttgta
     6901 gtaattcaga cctggctgaa tatccttttc gtcacctccg ttggcaatcg ccgcagccaa
     6961 tacagcaaat tgtgcttggt tggcaatcgt cacatccccc acgctaactt gatagtcacc
     7021 ctcttgcagc tccttccagc tagtcggtgc ttgcttcaca ttctctttat cggtgataaa
     7081 ggaaatggtt cccgtatagc ttaacaacca atccccatca tcatcctttg cccaatccgg
     7141 aatttcctcc caatagttgg tcttataagg taaggttaac tctttgcttt cagccaacgg
     7201 accgaagctg ataccgacat caccaatatc ggcggttcca ttttttcctt ctgattcaaa
     7261 cttagccaat tcttctgcac tggacatatc cgtatccacg tgacttaagc cgtattcttt
     7321 ttcaaggtcg ttccaagtgc ctacccaatt tgcccaagta tccggcattc ccaagctggc
     7381 aatctctcct tcttccttgg ctttttctat aatcgtatct aaagagtcgt ctgccgcagc
     7441 cgattgcttc gttgcttcac ctgagccgca gcctgtcagc aggatactta ctgttgctgc
     7501 acaaaataaa ccgatagtca tgttacgaat tttttcattc attttcattc attcttctcc
     7561 cctttcgata attccattct aacaaagctt tgtaatgaga gaatcagttt cttgaaaagg
     7621 tttcatgaac aatgtaaagg ttgtatatgg tttgtaaatt tcgacgaatt cccgttaacc
     7681 ttctaaagct tttgcggtat gatagtagaa aaaaggaggc tccctatgca gtattatgat
     7741 cagcatctcc acacgtattt ttcctttgat tcagaggaaa aatttgaaaa ctatttagcc
     7801 tatcagccgg agtttttcgt ttctacggat cactttgatt taaacaatcc atctaccgga
     7861 aaagatgaca ttccggatta tccagcctat ataaacaaac tggactcttt aaaagaaacc
     7921 tattccacca aatttctgcg aggtatcgag attggcgttg ttccaggaca agaagagcgg
     7981 atcgtggatt acctcgctca gcatccttat gatttaaagc tggtaagtat tcatcaaaat
     8041 ggccgctttg attatatgga cgatgtggtt ttaaccaaag atcccattca aactaccaag
     8101 gaatattttg atcagatgtt gcaggtcttg acggattttc cagaaggaga tattttgact
     8161 cactttgatt atggattacg ccgcttcacc ttcacagcgg aagaattcgc ggagcatttt
     8221 gaggctcagc tgctaacgat tttcaaaaag gttgttgaac gagaattggc gatggagtta
     8281 aatgccaaga gcttcttgaa atacggcaac gaagaccttt accgttatgc cgttcctttg
     8341 tatcaaagtg tcggcggaaa gctctttacc ttaggctcgg atgctcatgt tgccgaagat
     8401 tatcagctgg gctttaaaaa aatgagtcag ctgttggcag actgtcaggt agaaaagttg
     8461 ctagtcattc aaggaaaaga acgccgcctg acgccgctgc cgattttgtg attcctatat
     8521 aatagaagaa agtggtgaaa aaaatgagca acctgtggga ctacaatcaa gaggcaccca
     8581 tccattattt aattgcgcga cattgggatg cgttgaaaat tgaggcggtt tgccgatcct
     8641 tgttagcagc cgttccaaaa caacaactgg aaaatttttt ggtggctgac agcttgcagc
     8701 gggaaaaagt tcaggcttat tttgcagcct ttaaagatca gcctttggaa tatttgcatg
     8761 cgcagtttca tcttttttat caagtagccg ccccagatga ctacaacgat ttacgaggac
     8821 agctgcaatt gacctttcaa gcggatgaaa ccgcctatac cgttttattg ggaatggctc
     8881 gccttggtga tcaggcgaag gtcgagtggc ggattttcga tatttgatgg caggatcaag
     8941 gcgtttcagt tgcaaacctt cctctctttc aattagaaaa accgcagcag gaagaagttt
     9001 gtgtgctggt caccgatgct ggtgaaattc gtctccgctt atttcctgac caagctcccc
     9061 tggctgtagc caactggcgc tccctagctg agcaaggcgt ttatgacaac acacctttcg
     9121 gctcgtgtca ttaaggaatt tgttattcaa ggtggcgctt tggatggttc tggtgatgaa
     9181 gctgtttcca gctatgaggg ctttttcgct gatgaagtgc atcgcggact ttatcatttt
     9241 aatggcgcct tggccttagg ggaccacggc ccccacacca acggcaacca gttttttatt
     9301 gtgcaaaaca caaaggcgca ggctgacctg ctaatgtaaa gcaagcatac ttggagcttg
     9361 gaggactacc ggagctggat ggtcgttata ctgtttttgg acaggtatac caagggttag
     9421 aaatcgtagc agcgatcgca gctcagccga caaacagtga agatgtaccg attgagccag
     9481 tgttcattcg acaaattaac ttcgaaaaaa catcgtaaaa agacactcac tgacttcagc
     9541 aaaagctcta t
//
LOCUS       DANKHL010000111         9492 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000111 DANKHL010000000
VERSION     DANKHL010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.115"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            11..538
                     /locus_tag="PW101_001871"
     CDS             11..538
                     /locus_tag="PW101_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDL2514267.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            601..1971
                     /gene="radA"
                     /locus_tag="PW101_001872"
     CDS             601..1971
                     /gene="radA"
                     /locus_tag="PW101_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDL2514268.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            2005..3162
                     /locus_tag="PW101_001873"
     CDS             2005..3162
                     /locus_tag="PW101_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDL2514269.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            3332..4792
                     /locus_tag="PW101_001874"
     CDS             3332..4792
                     /locus_tag="PW101_001874"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDL2514270.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     misc_binding    4789..5028
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5114..5650
                     /gene="cysE"
                     /locus_tag="PW101_001875"
     CDS             5114..5650
                     /gene="cysE"
                     /locus_tag="PW101_001875"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDL2514271.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            5647..7059
                     /locus_tag="PW101_001876"
     CDS             5647..7059
                     /locus_tag="PW101_001876"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDL2514272.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            7056..7451
                     /locus_tag="PW101_001877"
     CDS             7056..7451
                     /locus_tag="PW101_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDL2514273.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            7444..8304
                     /gene="rlmB"
                     /locus_tag="PW101_001878"
     CDS             7444..8304
                     /gene="rlmB"
                     /locus_tag="PW101_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDL2514274.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            8308..8838
                     /locus_tag="PW101_001879"
     CDS             8308..8838
                     /locus_tag="PW101_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDL2514275.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            8915..>9492
                     /locus_tag="PW101_001880"
     CDS             8915..>9492
                     /locus_tag="PW101_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2514276.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
BASE COUNT         3245 a         1726 c         2140 g         2381 t
ORIGIN      
        1 aggaaaaccg gtgagaaaaa gaggatttga agttatttct aaatataaag atcaaaatat
       61 caatctaccg cagcgcgcta cacatcatgc tgctggctat gattttgaag cagcagaaga
      121 ggtcgtgatt ccaagtatct ggaaagcagg ctggaaaaag gggttggaaa cagtcgcaga
      181 tggattgaaa aaagatgtat cagaaaatga aaaacaattg aaaccaaccc tcgtgcctac
      241 tggaatcaaa gcttacatgg gagaagatga atttttgcag cttgctaacc gttcaagcaa
      301 tccgttgaag cggtttttag tgttgacaaa cggagtaggt gtgattgaca gtgattatta
      361 caataatgag gcaaatgaag gccatatcat gttccaattc accaactttg gtttcaagga
      421 tgtagtgatc aaaaaaggcg agcgtatcgg ccaagggatc tttttgccgt ttttaaaagc
      481 agatcaagat caaacgactg ctcaacgtca aggcggattt ggttcttcca gtaaataaaa
      541 cgttaaagat acttaagctt gatttaatgt ctcatgatta ggataaaagg aggcggtaca
      601 atggcaaaaa aatcaaaagt acaatttgtt tgccaaaatt gcgggtatgt gtcgcctaag
      661 ttcttgggac gctgcccaaa ttgcggaaaa tggaatacga tggtcgaaga aatcgaacaa
      721 gatacaacgg accgcagaac tcggacaagt ttgacaggag aaaaagcaaa accaacaaaa
      781 atcgcagacg tcgtccctaa aaaagaacct cggatcaaaa caaaattaga agaactgaac
      841 cgtgtgttag gcggaggtgt ggtgccaggg tctatggtct tgattggtgg cgatccaggt
      901 atcgggaaat ccactttgct gttacaagtt tctcagcaat tagcagccat aggcggaaaa
      961 gtcttatatg tatccggcga agaaagcgca gagcagataa aattacgcgc ggaacgcctc
     1021 ggaagtatca acacagaatt ttatctatac gctgaaacag atatgaacga aatcagtcgt
     1081 gccatcgaac atatctcccc tgattatgtt atcattgatt ccatccaaac gatgacgcag
     1141 ccagacatca caagtgtggc aggaagtgtc agccaagtaa gagaaaccac tgcagaacta
     1201 ctcaaaatcg ctaaaacaaa tgggatagcc atttttatcg tcggtcatgt taccaaagaa
     1261 ggctcgatcg ccggcccaag aatgttagaa catatggtgg atacggtgtt atattttgaa
     1321 ggtgaacaac atcatagttt tcggattttg cgagcagtaa aaaaccgatt tggctcaacg
     1381 aatgagattg ggatttttga aatgcatgag catggattgg aagaagtcgc taatccttca
     1441 cagattttct tagaagaacg cctagacggt gcaactggct ctgctatcgt tgtggcaatg
     1501 gaagggacac gtccaatttt ggtagagatc caagcattag tcacacctac tatgttcgga
     1561 aatgccaaac gaacaacaac aggtcttgat ttcaatcggg tatctctgat catggcagtg
     1621 ctggaaaaaa gagctgggtt gcttcttcag aatcaagatg cttacttaaa agctgcaggt
     1681 ggggtaaaat taaatgaacc agcgattgat ttagctatcg ctgtcagtat tgcttcaagc
     1741 tataaagaaa aaggaacaca gccaacggaa tgtttcatcg gtgaaattgg actgactgga
     1801 gaaatcagac gtgtcagtca tattgaacaa cgagtcaaag aagtccaaaa actcggcttc
     1861 acaaaagtat acctacctaa aaacaactta ggtaactggg aagcgccaaa aggaatcgag
     1921 atagttggag tagcaacttt ggctgaaacg ttgaagcgtg tgttccgcta actaataaat
     1981 gattgtcgaa acggaggaaa aacgatgcaa aaacgtgtca ttactttgct gatgatcatt
     2041 gtcggcgcaa gcttgggtat ttcattttta ccctcagctt ggcaggcaat cggtcaacag
     2101 tcaaacggat ggctgaataa tacatttacc aacagcctgt taggcgcact tattttcttt
     2161 ttattatctt tgctgttggc caaacatatc tcagctatta tcaaacaagt agaacaaaaa
     2221 ctaagtgagg tcagcttgac ttatcttctc ttcggtgcga tcggtgcgat catcggattg
     2281 actttaggtg ttgtgatctc gacccctctt tacaatatgg aaattccatt cgtcaatagt
     2341 gtattgccga ttttactgat gatcatctta ggctatctgg gattgagaat gggaacgaca
     2401 cgaatcgaag aatggaagaa agtttttggt tcacgaagca aaaaaatcga gcaagaaaca
     2461 gctagtcaag ttgagagtca actgttagaa cgaaaatcag gagaaaattt ccataaatat
     2521 aaaatcttgg atacaagcgt gatcattgac ggtcggattt atgatattac aaagactgga
     2581 ttcttagaag gaaccttgat gatccctaat tttgttcttt acgaactaca atacatcgcg
     2641 gactcagggg atagtttgaa acgtgtgcgt ggtcgtcgcg gattagatat cctaaatgcc
     2701 ttgcaaaaag aagagggtat atctgtcgaa atgtacgatg gagactttga agatatcagt
     2761 gaagtagaca gcaaattgat caaattggct aagcttttag atggtgtcat tgtaaccaat
     2821 gactataact tgaataaagt ctctgaattc caaaatgtac ctgttcttaa tatcaatgcg
     2881 ttggcaaatg ccgtaaaacc agtcgtcatc cctggtgaaa cgatgaatgt tctagtagta
     2941 aaagccggga cagaacgcca acaaggagta gcctaccttg atgacggcac gatggttgtc
     3001 gtagaagatg gacagcatta catgaatgaa cgtatcgaag tcgttgtaac aagtgccctt
     3061 caaacagctg ctggacgcat gatctttgct aaacctgcac atgcaggacg aggaatcgac
     3121 aataaaaaag agacagagaa aaagactaaa caatcaaaat aaaaaagaaa ttcagccaac
     3181 tggtaaggaa attcaaccag ttggctttac tatttacctt agaaaaggta aaatttgtgc
     3241 tgagtggctt taaataaatg atatttgaga agccgtaact gacggaattt ttgctgttag
     3301 tcaaagtcta gtttgaaaga agaggaagaa aatgacgaaa gtacgcgtac gctatgcacc
     3361 aagtccaact gggcacctgc atattggaaa tgcaagaact gcattattca attatttgtt
     3421 tgcccgccac aatgatggag actttattat tcgtattgag gatactgacc aaaaacgtaa
     3481 tatcgaagat ggcgaaaaaa gccagttgga aaaccttgct tggttgggaa tgaattggga
     3541 tgaatctcct gaaaatcctg gagaatacgg cccttaccgt caatctgaac gtaaagaaat
     3601 ctatcaaccg ctgattgatc agctgcttgc tagcaatcga gcatacaaat gctattgcac
     3661 agaagaagaa ttagaagcag aacgggaagc acaacgcgca agaggagaaa tgcctcatta
     3721 cgcaggaaca tgtgcgaact tgacacctga agaacaagca gaaaaagaag cacaaggtct
     3781 agaatctgtt gtacgtttcc gtgtgcctcg caatacagaa tacgcattta cagatatggt
     3841 gaaaggcgct atttcgttcg aatccgataa tatcggtggc gactttgtca tacaaaaacg
     3901 cgatggtatg ccaacttata attttgctgt tgcggtcgat gatcatttga tgaaaatcac
     3961 tcacgtttta cgtggagatg accatattgc caatacacca aaacaactga tgatctatga
     4021 agcatttggc tggacaccac caaccttcgg acatatgaca ttgatcatca acagtgaaac
     4081 aggtaaaaaa ctaagcaaac gtgacgaatc aatcttgcaa tttatcgaac aatatcgcga
     4141 attaggctat ctaccagatg caatgttcaa ctttattgca ttattgggct ggtcacctgt
     4201 gggcgaagac gaaatcttcg aacaccaaga attgatcaaa atgttcgatc caaaacgcct
     4261 aagtaaatca cccgcagctt tcgatgcgaa aaaactgcaa tgggtaaaca accattacat
     4321 caaagcaatg gatctagatg catttactga catgtgtctg ccttatctaa tcgctgatgg
     4381 acgtgtagca gaaaacccag atgaaaagac aatcgaatgg gtgaagaaga tcgttagcct
     4441 gtatcaacct caaatgagct atgctgcaga aatcgttgat ctgtctacgc tatttttcaa
     4501 tgaacatcct gtattgaatg aagcagcaaa agaagtactt gctggtgaaa cagttcctac
     4561 tgttctccac gcattcaaag cacagctaga acaaatggaa gtagtagatg ctccaactgt
     4621 caaagctgcc atcaaagctg ttcaaaaaga aactggcgta aaaggaaaaa atcttttcat
     4681 gccaatccgt gtagctgtat caggacaaat gcatggtcca gaattgcctg aaacgatcga
     4741 attgcttggt cgtgaaaaat cattagcaca tttaaatcaa gtactgaact aaacacgatg
     4801 aacagaaaaa agtaagcagc tgatcattac aagagagata ccgggtggtg caaggtatca
     4861 atgaatggtt gccgaaatgc ctctgggagt ggtcttggcg aaacacagaa aacaggaaaa
     4921 actgttttat aggaaacaaa gtagccaaga acgtataaca ggcgttagct gtcagagata
     4981 atcattgatt atgaagaaaa gtggaaccac gcggaaagcg tcttttgata aggaaactta
     5041 tttaaaaggc gcttcttttc tttataagaa gatgatttcc tacataaaat tcttacgaaa
     5101 ggagtggaaa gtcatgagtt ggtttacccg agcgattgat gcagcaaaga gaaacgatcc
     5161 ggctgcacgt tcgacagctg agatcgtttt gacataccct ggttttcatg ccttgttttg
     5221 gcatcgtttc tctcattttt tatatcagca ccggctcttt ttattggcga agatcaatgc
     5281 tcaattctgg cgtttcctaa ctggtgttga gatccatcca ggagcaacga ttggaactgg
     5341 cgtgttcatc gaccatggaa tgggaatcgt cattggcgaa accgcagaaa tcgaggacga
     5401 tgtgatttta ttccatggcg taacattagg cggcacagga aaagaaacgg gtaaaagaca
     5461 cccaactgtt aaacaaggag ccatgctctc tgcgaatgcg cagatattag gaccggtaac
     5521 gatcggaaaa aacgccaaaa tcggtgcagg tgccgttgtg ttgaaagatg ttccagacga
     5581 cgctacagcg gtaggagtac cagcaaaagt cgttcgaatc aaaggagaaa aagtgaggag
     5641 atcagaatga taaaaatcta caatacattg accagaaaaa aagaagaatt catccctata
     5701 caaaagggac atgtcggtat gtatgtctgc ggaccgactg tgtataatta tatccatatc
     5761 ggaaatgctc gtagcaccat tgctttcgat acaattcgac gctattttga atatcgaggc
     5821 tataaagtcg atttcgtatc gaatttcacg gatgtagacg ataaaatcat ccgtacagca
     5881 aacgaattag gtattacagc accagaagtt gctgatcgtt tcatccgagc atttgaagaa
     5941 gatacgaaag cgttgaatgt cgagcctgct acactgcatc caagagtcat ggatcatatg
     6001 ccagatattt tagcatttat tcaagtgttg gttgaaaaag gctatgctta tgaatcttca
     6061 ggtgatgtgt attaccgtac ccgtaaattt gaaaattatg ggaaattaag tgaccaatcc
     6121 atcgatgaac tggaagtcgg agcaagtcag cgtacagggg aagaacagca gcaaaaagaa
     6181 gacccattag atttcgcttt atggaaacaa gcaaaacctg gcgagatttc ttgggattca
     6241 ccatggggca aaggacgtcc cggttggcat attgaatgtt ccgttatggc aacaaaacat
     6301 ttaggagaaa cgatcgatat ccatggtggc gggcaagatt tagaatttcc tcatcatgaa
     6361 aatgaaatcg cacaaagtga agcgaaaaca gggaaaaaat tcgctaatta ctggatgcac
     6421 aatggctatg taacgattgg cgaagacgat gaaaaaatga gtaaatctct agggaatttc
     6481 atcactgtac atgaaatgat caaaaacgtt gatccacaag tcttgcgttt cttcatggcg
     6541 accacacaat accgtcgtcc gatccgctac agtgaaacga cgatgaaaga agcagccacc
     6601 aatttacaac gactgaagaa tacgtttgac aatttgaaat tccgccaaga aactgcctca
     6661 gagaatttgg cagatgatgc ggataaaatc gctttgttag aaaatttaga acagcgattc
     6721 atcgaagaaa tggacgatga ttttaatgca gcaaatggga tcacagtggt ctatgagctt
     6781 gctaagtggc tgaacactta tctagaccag ccgaaagttt ctgccgctgt cttggcacaa
     6841 ggagaagctt tattctcgca atggctttcg atcttcggta ttttatttac agcagaagag
     6901 ttattagatg aagagattga acaattgatc gaagaacgaa atcaagcaag aagcaatcgt
     6961 gattttgtcc gaagcgacga aattcgtgat ttgttgaaag aaaaagggat cttattagaa
     7021 gatacaccgc aaggaacccg atggagaaga gaagcatgag agattacaca caattgaacg
     7081 gtttagcctt ggcttatgtt ggagatgcga tttacgaaat ctatgttcgt gaccacctga
     7141 ttgatcaagg agaaacaaaa cccaataagc tgcatcgtgc ggcaacgaat tatgtttctg
     7201 cgaatgcaca agcctttttg attcagcaaa tgctggaaga acatcttttg acagaagaag
     7261 aagaacttta ttatcgcaga ggaagaaatg ctaaaagcca tacgagcgca aaaaatgctg
     7321 atgttacgac gtatcgggtc gcaacagggt tcgaagcgct catgggctat ctgcatttga
     7381 caaaacaaac cgagcggatg gaagaattga tccgctggtg tattcagaaa gttggtgaaa
     7441 aaaatgggta aagaaagaac aaacaaacat caatcgaaac gtggatttga agacaaaaaa
     7501 caaaatagat attcaaataa aaagcaaact ggacgaaatc agtcagtttc tgatcaaaat
     7561 gaagaagcat tagaagataa tttcgtcttt gggcaccatg ccgcagtaga agcgctacaa
     7621 caagggcgag ggaataaact ctttcttcaa gaagattcac gaggagaaaa agtagaagaa
     7681 ctgaaagaaa tcgcacgtga acaagctgtg ccagtcaaat gggttcctaa acaaaaatta
     7741 gaaactttga gcgatcacgg cgtgcatcaa ggagtcgttc ttgcgattac accttatgaa
     7801 tatttatccc ttgaccaact gattgaacga acaaaagaga aaaaagaaga ccccttcttt
     7861 ttgattttgg atagcatcga agatccacac aattttgggt cgattttacg gacagcagac
     7921 gcaagtggcg tcgatggtgt gatcatacca aaacaccgag cagttggaat cacacctatc
     7981 gtaacaaaga cttccactgg ggcagtcgaa catgtccctg ttgcacgcgt gacgaatttg
     8041 gtacaagctg tgcaacagct caaagaaaca ggattttggg tatttggcac agatatgacc
     8101 ggcacaaact tccaaaaatg gaatgccaaa ggatcaattg ctttagtcat tggtaatgaa
     8161 ggcaaaggaa tgagtccagg attgaaaaaa gaaatggatg aattgctgac gatcccgatg
     8221 accgggcacg tccagagctt aaatgccagt gttgctgctg gtttgcttat gtatcaggct
     8281 ttttcccaac gtcaggggga gtagaccatg aaaaaacagc tgttgattgt agacggctac
     8341 aacatgattg gttcgtggcc ggaacttgtc cagcttaaaa agcaggacaa aatggcggat
     8401 gcaagagaag ctcttttaca tcgtttatca aattatgcga aatatgaagg aatggaagtc
     8461 atcgttgtct tcgatgccca actagttccg ggcatccaac aaaattataa gaaataccag
     8521 ttggatgttg tttttacgaa agaagacgaa acggctgaca gttatatcga gagaatcgct
     8581 ggtcagctga atgaccgttt gacacaagtg acagtggcga caagcgattt agcagaacag
     8641 tggatgatct tttcccaagg agcattacga acttctgctt atgaattata taagacgata
     8701 aaaaaaacgg aaaaaacaat agcccaagaa aataaagata tgcattttat tgatttccga
     8761 cgtaattctc catggaatga agagcagtta aaaaaattgt cccaaaaatt ggacgattta
     8821 atgagaaaaa ataaataaca tatcgttcta atcagttcga tgaatcattg atgataagat
     8881 tcctccataa acgaatttat ggaggaatta ttttatgtct gaggagttat tgcgcgctgc
     8941 acgtaatggt gacgaagaag catttgctgc cctgtatcaa aaatattttc cacttttact
     9001 acgtttgaaa aacacctatt atttaagaaa ttatgtttct gaggattggg aacaagaagc
     9061 aaggatcgct ttatatcagg cattgagaac ttacgaaaaa agcaaaggtg ttagctttgg
     9121 aagttattat aaaagcatta taagcaatca aatctattct ctgttgagga agcagaacgc
     9181 gctgaagcgt cgtgatcaaa aagatgaagt ctcaatcgac caaaaaatag agacagaagg
     9241 accagacttt ttagcagaga tcacattgca agaaccttta gcgattcagc atcttttatt
     9301 aaaagaagca atcgaaaatt gcgaaatcca gttttcaaaa atagaagcta aaatttttca
     9361 ttattatatg caaggcgttg attttgtaga catggctgaa tgtttagcaa tggatcacaa
     9421 gcaagtaatg agcgcttacg ggagagtaaa actcaaaata aaaaaacact tgaaatttta
     9481 taatgaggaa ta
//
LOCUS       DANKHL010000112         9469 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000112 DANKHL010000000
VERSION     DANKHL010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.116"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(264..2390)
                     /gene="pnp"
                     /locus_tag="PW101_001881"
     CDS             complement(264..2390)
                     /gene="pnp"
                     /locus_tag="PW101_001881"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDL2514277.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(2578..2847)
                     /gene="rpsO"
                     /locus_tag="PW101_001882"
     CDS             complement(2578..2847)
                     /gene="rpsO"
                     /locus_tag="PW101_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDL2514278.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            3098..3661
                     /locus_tag="PW101_001883"
     CDS             3098..3661
                     /locus_tag="PW101_001883"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL2514279.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(3914..4435)
                     /locus_tag="PW101_001884"
     CDS             complement(3914..4435)
                     /locus_tag="PW101_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDL2514280.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(4438..5280)
                     /locus_tag="PW101_001885"
     CDS             complement(4438..5280)
                     /locus_tag="PW101_001885"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDL2514281.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(5273..5998)
                     /locus_tag="PW101_001886"
     CDS             complement(5273..5998)
                     /locus_tag="PW101_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDL2514282.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            6174..6470
                     /locus_tag="PW101_001887"
     CDS             6174..6470
                     /locus_tag="PW101_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514283.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            7038..8630
                     /locus_tag="PW101_001888"
     CDS             7038..8630
                     /locus_tag="PW101_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2514284.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            8643..9383
                     /locus_tag="PW101_001889"
     CDS             8643..9383
                     /locus_tag="PW101_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2514285.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         2641 a         1921 c         1878 g         3029 t
ORIGIN      
        1 cgagggacag ctgattgaac atcgtttatt ggagaggttg taaaaaagct gttctgcatc
       61 aagtaataag aacagccaag taaaaacagc tcctaatgtt tttaacttgg ctggacttat
      121 taccgcagat gcaagctttt gaacaccgtt tattcgaaaa aaaaggactg tgacacggct
      181 tttgtcacag tccccttttt tcgaataatt attgtccccc tgtcaccttc acgatcacaa
      241 gtacattatt cctatgaatt aaattatttt tcttcatttt tcgttgaacc atcttcgttc
      301 aacattgctt ttcttgacag atttacgcgt ccttgtttat cgatttcagt tactttcaca
      361 aggatctcat cacctagttt aacaacatct tcgactttat tgacacgatc gtttgctaat
      421 tgggaaatat ggacaagtcc atcttttcct ttgatcaagt tgacgaatgc accgaatttt
      481 tcgatacgga caactttccc tagataaact tcaccaactt tgacttcttt ggtcaaatct
      541 tcgatgatct tgatagcttt tttgatcatt tctgcatcag ctgaagcaat acttactttt
      601 ccatcttgat cgatatcgat tttcacacca gtttcatcga tgattccgtt gatcgtatcg
      661 ccgcctttac cgataacatc tttgatttta gcaggttcga tctggatcat ttcgattttt
      721 ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg ttgaagtcaa ttcttctaag
      781 atttccatac gtgcttgttt tgcttgagtt aatgcttcag ttaggatttg ttctgtgatt
      841 ccttgaatct tgatatccat ctgtaaagca gtgataccat cttttgtacc ggcaacttta
      901 aagtccatat cgccaagatg atcttccagc ccttggatat cagttaggat cgtatagttt
      961 tcaccatcag aaacaagtcc catcgcaata ccggcgactg gcgctttgat tggtacacct
     1021 gcatccatca atgctaatgt gccagcacag atacttgctt gggaagaaga accatttgat
     1081 tccaatactt ctgctactaa acggatcgta taaggaaatt cttcttctga agggattact
     1141 tgtgctaacg cacgttcacc taatgcgcca tgtccgattt cccgacgccc tggagagcct
     1201 gcacgtcctg tagaaccaac agaaaattga gggaaattgt agtggtggat gaatcgtttg
     1261 ctgacttcca cccctaaacc atcaatgatt tggtgctctc ctagaggtgc gagtgtacaa
     1321 gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg atccatgtac acgtggcaat
     1381 aagctgacct ctgatgacaa tggacggatt tcatccaatt tacgcccgtc aggacggatc
     1441 ttatcgatgg tgatcaattc acgtacgaca tctttttcaa gatcttctgt gatttgttta
     1501 acttctttca acaagtgatc ttcatcttca tgacctgcaa atttctcagc ataagcttcg
     1561 cggatatcga tcttcacctg ctcgatatta tcttcgcgag caagtttttc ttcagtcatt
     1621 actgctgctt tcattgtagc gtaagaagca tcaaagattt cttttttcaa tacttcatca
     1681 acttgcagta aagtcacttc cattttttct ttccctactg cttgaacgat ttcttcttgg
     1741 aaagcaacta actctttgat agcgtcaaaa ccaaacagca atgcaccaag catgtcttct
     1801 tctgaaactt ctttcgcgcc gctttcaacc atattgatgg cttgtttcgt tccggcaaca
     1861 gtcaattcga tatctgtttg ttctgcttgt tctactgttg gattcaagac gtactcgcca
     1921 tttacacgac caacttctac gcctgcgatt gggccgtcaa atgggatatc tgagattgat
     1981 agtgctaaag aagaacctag cattgctgcc atagctggcg agcagtctgt ttctacactc
     2041 atcacgatgt tcgttacttg gacttcatta cggaagcctt ctgcaaacat cgggcgaatt
     2101 ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac ttggacggcc ttctcgtttg
     2161 atgaaacctc cagggatttt ccctgctgca tacatttttt cttcataatt gatcgttaat
     2221 gggaaaaagt ctgtatcttt tgcttcttta gaggccacag ctgcacttaa gactactgta
     2281 tctccataac ggactaaaac ggctccgttt gcttgttttg ctagctgacc gacttcgatt
     2341 tgtaagggac ggccgcccca tgtcgttttg aatacttgtt tttctgacat aacttctcct
     2401 tttccttctc gtgaaagcaa gacgctacta aacaaatgaa aactgccgaa agccgtcttt
     2461 tgagcagctt tcatttggtt agtaggagtc catctagctt gatcacggaa ttttacttaa
     2521 ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc actctttttg actaaaatta
     2581 acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt tgaacgtcag ttttacgtaa
     2641 gtaagctaac aagttacggc ggtgaccaac ttttttcatt aggccgcggt atgaatgatg
     2701 gtcttttttg tgcacgcgtg catgttcatt caagtggttg atttcttcag ttaatacagc
     2761 gatttgtact tctggagaac cagtatcccc ttcatggcga gcgtattcgt tgatgatttc
     2821 attcttgcgt tcttttgaaa ttgccatttc tttcacctct ttcgattatt cccctgcgac
     2881 tgagtaaaac gttggtgatt cgcttaacca agtggcaggt catgtgccta gagcacacag
     2941 ctatacttta cctgaaaccg ggttgaaaat caagtagtta atcattaaga aaaggtagtt
     3001 ttcaaacaat cttactacta cttttccatt tttgtggtaa actataatgc gaaaatagac
     3061 ttgaaacaca gtcttttaag aaaggggtac atattccatg attacaatgg atgatatcat
     3121 tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa gtttctcttc cattaagcga
     3181 agaagatatc tctttaggaa aagagatgct tgagttctta aaaaacagcc aagatccaat
     3241 caaagctgaa gaactacact tacgaggcgg tgttggctta gctgctcctc aattagatat
     3301 ttctaaaaga atcattgctg ttcacgtacc aagtcctgac ccagaggcag atggaccttc
     3361 tctaagtact gttatgtata atcctaaaat tttgagccat tctgttcaag atgcttgttt
     3421 aggggaaggt gaaggctgtt tatctgttga tcgagaagta ccaggatatg ttgttcgcca
     3481 tgccaaaatc actgtgtctt attatgatat gaatggtgaa aaacacaaga ttcgtttgaa
     3541 gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat atcaacggtg tgatgttcta
     3601 tgaccacatc aacgatcaaa atccatttgc actaaaagaa ggcgtattgg tgatcgagta
     3661 gagaattggc aggttccttc gatactatgc tacaagaaag ctttataaaa aaggggagtg
     3721 ctcaaaagct gcacttccct tttttatgtt tctccactaa tcgaaattta gatagacatc
     3781 tagaaatcgc aattccaaaa aattctgtgt attggatacg catcttttct tattttttct
     3841 tatttcttcc atatgggtgt ttatctcaga gatgatagaa tagtttctag gataaaaagt
     3901 agcagctacg atgttatcct tcgtagagga ttcctgcttc agctagtttt tgtttgatgt
     3961 ttttcagcat atgcttttct gggtagcgga tcgtatgcag atggatgccg cctgttaatt
     4021 cggataacaa agtagcatta cttttttgat aacgtctcat aaaggaatcg atatcttgtg
     4081 gcgttttgat gtgcaaagtt cctgctaact caccgtaaag gggatgttcc acaattacat
     4141 caatgatttc accaccattt tcaacgatta gtctcagttc ttcttctgtt tgatcttttc
     4201 catgctggac agcgatttta ctgatgtgcc cagtattggt ttcttctgtt tcgaggatat
     4261 aacctcttgc tgttgccacg atacttttac ctgcagctcg tagtaaagca atatcgccta
     4321 caatgatctg acggctgact ccaaaagttt tcgcaaactt gctagcactc actggttttt
     4381 ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt ctctccttcg atcacactta
     4441 ttcctccttc attttgtttt ggtattcgcg cgagtgtttc atcaaatcaa catacgtgtc
     4501 tgttattgtt tccttatatc ccttattttt cagcagacta acaactttat ccaatacttg
     4561 ctgctctctt tttgcatcat aaactgcttg gttttcttct tttttcactt gtcctatttt
     4621 agttaccgta tccattcttt tttctaataa agcaaccaac tctcgatcga tcatatcgat
     4681 cttatgacgt aatgtttcaa gagactcttc tgttagttct tcctctactg tcttgatgat
     4741 acgagcagga tttcctgcaa tcactacatt gtcgggaaaa gatttggtca caaccgaacc
     4801 tgcgccgact actacgttgt cccctagagt gacacccggc gtgacgataa ctcctccgcc
     4861 taaccaaaca ttattaccga ttttaatggg ttttgcgtac tccagaccgc tgttacgttt
     4921 cactggatgt agtggatgcg tcgccgtgta aagctggaca ttcggagcga acatgcagtt
     4981 atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa ttgaaatttg cataaaaatt
     5041 ctccccaaca aatatattat atccgtaatc aaaattgata actggctcca tatagatttt
     5101 tttccctgta cggccaaaag tctctttcaa caattgggat cgcagttccg ctgattctgc
     5161 ttggttgatg atttggcttt gacttcttgc aaacatacgg tcggcaacca gctcaggatc
     5221 attggcaaaa taaagctcgc ctgcaatcat tttttctttt tcggttttga attcagtcat
     5281 aatctgctcc taattcttgt atttttttga aaacaaccgc tccgtcttct tgaataaacg
     5341 tataaggctc cgctaaataa acaggcgttt gattgattac taagattttg gctgacggct
     5401 ggcgaaactg taccagatca caaaacggat gaacttgaaa actcgttcca acaatgacga
     5461 tcagctctgc ttggctgacg gcataagccg cctgctccat gactccatct tgaaaccctt
     5521 caccatataa cacgatgtct gggcgtattt gtcctccgca tgcttgatgt ttgtcgctgt
     5581 gaagatattc tttccagtct accgtttgtt gacattttcg acaatgacat tgatacagac
     5641 tcccatgaaa attgacgagt tgcgggcttt tagcctttgc gtgcaaaccg tcgatgtttt
     5701 gggaaacaac ccaaacattt ttttctttcg ctagatgtgc tatttccaaa tgaatgatat
     5761 tgggttttgc ttcaggatga taaatctttt tgacaaattg atagaattta tccggttcat
     5821 tcaccattgc ttgatgactc agcaagtatt ccggctgctc catcccgtgg tagattcctg
     5881 acaaagatcg atagtctggc actccagaag gtgtggatac ccctgcaccg gtcaaaaatg
     5941 taatttgctg ataagctaat atctcttttg ctgcttcgtc tactgttaat tgtttcatgt
     6001 tttgtcacct cactttccat tatagcccat actcgaacaa atttattgtt gaaaagtcag
     6061 aaaaatggga ataaaaaagt gttatgggca tttgagcaat ttatcacaat gttcactgac
     6121 aaaagaatga aaacgtgtta caatactgct gtataaaagt gaggtgaatc gatatgttac
     6181 cattctttat ttattggggc gttattctgg cttgtatcgc ctggttagcg ttaagcgttt
     6241 atttctctgt tttttatctt gtacgcaaag aaaatggaaa tctttgggct tttgctttat
     6301 tcaacgtgat tgccgcaatt gttttagcca ttacattagc cgtatatcga acatggggct
     6361 ggggaatcac ccaatactca agtttgattt atctaatatt agccatttat ggcgtcgttg
     6421 ttcttttgca agcgatccta ggacgtgagc caaagaaagc ggcagcttaa atgatcgaga
     6481 tggaactgga cacaggaaat tttctgttct agttcttttt ttattgtatt acatacattt
     6541 ttgtgtataa ttatactatc gtttgcaatg gatagatgat tcacattttg attgtttttt
     6601 cggtaaaagt aaagatttct atatttatca gtattttttc aaaaaccagc agttatagat
     6661 tgacaaagcc tctcgagtat cgtaaacttt ttttgtataa ataactgaat acgagatgcc
     6721 tcaatcaatg aggtagaggt cgcgagaatc attaaccctg tggagtgaag caaacatgga
     6781 gaagcaggaa tagggaggat cgccgaaatg tgcacttttt tgcttggagt gtatgttggg
     6841 acgcaagtta agagcttgcg gactgtctta gcagtgatgc taagttgcgc tatgttttgt
     6901 gagaggttta tcaccttatg gaattttagc ggccttttta tatctataaa tcggtcgctt
     6961 tttatttttt cagataaagc tttaccgcat ttcatctaga ccgctcgtgt atcatataat
     7021 cagaaaggga gaaaaaaatg aaaaaaaata tggtttcagt tgccgtacta tttgcttttg
     7081 tcttctcact attcagtgtg ggatctctgg caaatgcaga tgaaaaaaca ccgagcgatg
     7141 agtttcgcgt aggtatggag gctgggtatg cacccttcaa ctggtctcaa caaacagatg
     7201 agaataatgc cttgccaatt caaggccaaa atagttacgc cggcggttat gatgttcaaa
     7261 tcgctaaaaa agtagcagat ggtttaggga aaaaattagt catcgtccaa acgaaatggg
     7321 atggtctggc tcctgccctt caatcaggca agatcgacgc gatcatcgcc ggaatgagcc
     7381 ctactgctga acgacgaaaa gaaattgatt ttaccgatcc ttattacgaa tcacaattag
     7441 tagtagtagt tcaaaaagat agcaaatatg caaaagcaaa aagtttagca gatctttctg
     7501 gagcaaagat cactgctcag ttaaatacat tccactatac tgtgatcgat cagataccag
     7561 atgtgcagaa acaacaagca atggataatt tctcagcaat gagaacagct ttagcttctg
     7621 gaatgatcga tggttatgtc agcgaacgtc cggaaggtgt aacggcaact agtgtgaaca
     7681 aagacttgga aatgcttgaa ttcagcggtg cagatggctt ccagacaaac ccggaagatg
     7741 ttcagatttc tgttggtatg cgtaaaggcg acccggatat ccaaaaagta aaccagattc
     7801 tttctggcat ctctaacgat gaacgaacca aaattatgga ccaagcagtc aaagatcagc
     7861 ctgctgcaac tgatagtgaa gaaaaacctg gcatcatttc tgatttcact acgatttgga
     7921 aacaatacgg caatatgttc ttacgaggtg ctggacttac cttgttcatc gcattgatcg
     7981 ggacagttgt cggtactaca cttggattac tgattggtgt cttccgttcg gtacctgaat
     8041 caggaaatgc actgactcgc ttcttccaaa aaatcggaaa tgcacttctt tctatctatg
     8101 tcgaagtatt ccgcggaaca ccgatgatgg tacaagcaat ggtcatcttc tacggtctgg
     8161 cactcgcttt tggcatttca ttgaaccgaa cgattgccgc tttgtttatt gtctctctca
     8221 atactggagc ttacatgaca gagatcgttc gtggggggat ttttgcagta gatgaagggc
     8281 agttcgaagc tgcgcaagcc attggtatga cccatggcca aacgatgaga aaagttgtta
     8341 ttccacaagt tttacggaat atcttgccag caacaggaaa tgagtttgtt atcaatatca
     8401 aagatacagc agttttaagt gtaatcggcg tagctgattt gttcttccaa ggaaactctg
     8461 cttccggtgc gaatttccaa ttcttccaaa catttacgat cgtcgggatc atctacttag
     8521 tcatgacctt tacgatcacc agaatcctgc gggcggtaga aaagaaaatc gatggtcctt
     8581 cagcttatac caagcttgaa gaaatcgaca attcaaattt gcaggaataa ggaggaagaa
     8641 tcatgaatga aaaaattatt gaaatcaaac atctaagaaa aagcttcggt gaaaatgaag
     8701 tattgaaaga tatcagcgta acagtcaata aaggtgaagt tgtgacgatc atcggttctt
     8761 ccggttctgg taaatctact ttcttgcgct gtatcaattt actggaaaaa ccaacaggcg
     8821 gcgagatcat ttataatgga gaaaatgtat tagctcctaa atataattta cctaaatatc
     8881 gaacgaatct aggtatggtt ttccaatcct tcaacctgtt caacaatatg aatgtcttgg
     8941 aaaactgtat gtctggtcaa acaactgtcc tgaaacgaga taaagaaact gccaaaaaag
     9001 tagcaatcga aaatctggaa aaagttggta tggatcgtta tatcgaagcc aaaccagcac
     9061 aattatctgg tggacagaaa cagcgtgtcg caattgctcg ggcattatca atgaatccag
     9121 atgtattgct attcgacgaa cctacttcgg cgttagaccc tgaaatggtt ggcgaagtgc
     9181 ttaatacgat caaagactta gcacatactg gcttgacaat gcttatcgtg acgcatgaga
     9241 tggaatttgc aaaagatgta tctgaccgtg tcatttttat ggataaaggt gtgattgcag
     9301 aagaaggtac accagaagat atctttgtcc atccgaaaga agaacgtaca aaagaattcc
     9361 tttcacgtat tttaaatgct taggatcaat agctgattgg gacaaagagc tgcggtgttt
     9421 accgcagttc ttttttatga attactcata aaaggtaaat ttctgaaaa
//
LOCUS       DANKHL010000113         9432 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000113 DANKHL010000000
VERSION     DANKHL010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.117"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..680
                     /locus_tag="PW101_001890"
     CDS             <1..680
                     /locus_tag="PW101_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2514286.1"
                     /translation="YFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHI
                     VQHIGRTFLNHRVKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRAS
                     FKDYLTESEIVDRLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETY
                     TTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRH
                     GKTFLTK"
     gene            800..1000
                     /locus_tag="PW101_001891"
     CDS             800..1000
                     /locus_tag="PW101_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2514287.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            1076..1273
                     /locus_tag="PW101_001892"
     CDS             1076..1273
                     /locus_tag="PW101_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514288.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(1570..3117)
                     /locus_tag="PW101_001893"
     CDS             complement(1570..3117)
                     /locus_tag="PW101_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDL2514289.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(3201..4004)
                     /locus_tag="PW101_001894"
     CDS             complement(3201..4004)
                     /locus_tag="PW101_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2514290.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="PW101_001895"
     CDS             complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="PW101_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDL2514291.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="PW101_001896"
     CDS             complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="PW101_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDL2514292.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="PW101_001897"
     CDS             complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="PW101_001897"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDL2514293.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6389..7318)
                     /locus_tag="PW101_001898"
     CDS             complement(6389..7318)
                     /locus_tag="PW101_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL2514294.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            7513..8196
                     /locus_tag="PW101_001899"
     CDS             7513..8196
                     /locus_tag="PW101_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HDL2514295.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            8357..9400
                     /locus_tag="PW101_001900"
     CDS             8357..9400
                     /locus_tag="PW101_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2514296.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         2893 a         1933 c         1869 g         2737 t
ORIGIN      
        1 gctatttttc tcggttttca ttagcgactc gtgaagccgt tcaatggatt gttattgata
       61 tgtatgcgcc ttatgtttca ttggtgaaaa agcttttccc gaatgctcag ttgatcatcg
      121 accgtttcca catcgtccaa catattggca gaaccttctt gaatcatcga gtgaaagaaa
      181 ccacggcacg gcttaaagga acttcccact ctcaacgagg gttagggaaa aaattgaaac
      241 gctattggaa actcctacaa aaagaagaag caaagttaaa ctacgaaaaa agggtttggc
      301 gtgccagttt taaagattat ttgactgaaa gtgagattgt cgatcgactg ttatctgctt
      361 gtcccaacct gcgccaaggc tatcagttat accaagacat tctttatgcg gtaaagaaaa
      421 gagatcagtc attatttgag gactgtttaa acagagaagt cagcggtctt cctgaaacct
      481 atacaaccac tttacgaacg tttaaaaagt ttctgccaca gatccaaaat gcgcttcact
      541 acagctattc aaatggacca ttggaatgtt taaacaacca catcaaagta ttaaaacgca
      601 atgcgtacgg ctttcgcagc ttttacaatt tcaaactacg gatcatgatt cgtcatggca
      661 aaacattttt aaccaaataa acagaacaga aagagagttt cttctttctg ttctgtccaa
      721 attattctaa gatttctctt taccaacatc atttgacaaa gagcctgtat ttattactgt
      781 cgaggacaat ggcatcggca tgattgaaga ggaacaaaaa aacatttggg atcgctatta
      841 taaagccgat ccgtcaagta aaaatacaaa gataggcgag tcaggtcttg gattatcaat
      901 cgtgaaggaa ctagtgcgcc tgcaccacgg ttcaattgaa gtgaccagcc gaaaaaatga
      961 aggaacgacc ttcctgatac aatttccaaa agaaaaataa aattaccagt aaaaaaccgt
     1021 ggagagatag ctgctattga tttttgctat gtcttttctt tgagaggtat ccggtatggt
     1081 ggagtgtctg aaaaataaaa aaatggaggc caattttgtg aaagctgtta tttctggaag
     1141 tattgttttc caagaagaat atcgagatat ggaaaaagat ttcagtaaaa aaggcattga
     1201 attaattgat tatcctcgct catgtccgat cctagacaaa gagtatccag aaatcttgac
     1261 gacttttttt taaatatcgc agcagcggat ttattttttg tatttaatca agatcggaaa
     1321 ggggtattag gctatattgg tgccgcaact ttttcagaaa tgaagtattg tctgatgcaa
     1381 aatcttattc atggaaaaag gatcaaaata gtcttattta aggaaccatc agaggaaaat
     1441 tttttgtttt gatgaggtag atcgctggct aaagaatggc tggatggaaa tcagacagcc
     1501 taacaatttg gcataacaaa ggtgtgggca aaaaactgct cacacctttt tgaatttttt
     1561 atttatatac tacaaaatca cttgaaaatt atgattcact tttgcttcga tgcgtgggtg
     1621 aatattcaaa tagtcggcaa ttaattcggt catgtcgatt tggatttctc ggacaatttt
     1681 ctccgcacca aacatcgcat aatttcctcc gccgattgcc cgatattgat ttgtcaccac
     1741 ttccagcatt tgttgcggaa gaacggggtg acctttaaaa cgaagttcgg taatccgttg
     1801 tccaacaggc tgtttcatat caatagtgta ctgaatccct tcatacatgt cataattgta
     1861 gtactggggc ttaggctgaa taaaggctgg attgaatacg atttggttat tttctacagc
     1921 taaataattt gcggtctgct ccaacgccgc tctcaaatct gcaccattta tttgaatcac
     1981 tgccagagta ttcggataaa tatagttggt gatgacgtcc cgcatcgtga tcgtctcacc
     2041 aaagcctttt ccttcattat tgaataacgc agttccagaa atctctgagc cgctggcaaa
     2101 taattgtaca cgattaataa actctacata gggatgttcc tcaagacgag cctgcatcgg
     2161 atcattaata cgcatatttc cgttcacttt acctaagggt tgatccaacc atttctctag
     2221 tttttcttct accggtacga tcgctgcaag aatttctgga tcaggctctg atttttcaac
     2281 tgagtgtaaa gcaacctggt gatctgtaac aaccacttgc ccctcttttt tctcgacggt
     2341 aagacgaatt tcaccgatga actcgccacg atagcctggt tgaacaacgg gaactccatg
     2401 aaccgtttca gcaattttcc ggtgctgatg accggtgaaa agggcgtcaa tgccttcaac
     2461 ctgctgcaat agctcgtagc cttcattctc tccagttagc gcttctgtgt cctcaccggt
     2521 taacaagtct ttttcaaatc cgccgtggta actaacgatt accaagtctg cttgtttcct
     2581 taatagagga acatattctt ttgctgtttc gacgatactc ttaaacgtaa ttcctgctaa
     2641 ggtttctggc ttttcccagt gaggaatgta ttgtgtcgtt aatcccaaga tagcaatttt
     2701 tacacccacc ttttcgataa tcttataggc gtttccaaag tatggttggt catttttgtt
     2761 caccatattg gctcctaata taggcgcttg ataattctta atcgcttcct cgagataatc
     2821 cacaccatag ttaaactcat gatttcccaa cacttgaaca tcatagctca tttggttaat
     2881 caagtctgta agctctttgg ctgaaccata ttctttagca agataataac tcaaaggaga
     2941 cccttggatg aaatcaccat tttcgatcgt aattaccggt ccatctgctt ttgcccgtaa
     3001 ttccttcagt ttcgacgata ctttagccgt tcctaaggcc agatcggttc tgccggaata
     3061 gtctgttggt gaaacatagc catgcatatc acttgtctct aaaaacgtaa tgttcattct
     3121 gcgtgctcct ttagctgact caaaatagaa aaaccgaagc gtattcagct gttcggtctc
     3181 tttttttgat ttataatgcc ttaatccttc atttgaagtg tttttaaaat gttgcctgga
     3241 ataaagtttt ctgttgtctg gcgaacaatc cttccttgat cgagatgata aaggttatac
     3301 ccatgacgtt cttcaaagcg tacgaagcca tcgccttcat cttcaccaac aaaggacata
     3361 ctcgatacag taaaataggg gatcccatta atttcatcag taaatgtgtg atgggtatga
     3421 ccgtttagga tacagcgaat atcgtaaggt gcgattagct tcaagaaacg ttcggtacca
     3481 ggccattttg gtatactgct ttggtgagac agtagatgat gatgggtgac caagaaaatc
     3541 ggttggtctt tttcttgctc taaggctttt ttcagccatt gaaattgctg atcgtccact
     3601 ataccatctg cgtatccata gcttgaatta tcaaaggaaa tcactgtaaa ctcaggatat
     3661 ttgactatct ggttgtacgg ggcagaagag gcggcttcat cgcaccagcc tacccaaaaa
     3721 tgttctttga tatcgtgatt gcctagagta acaagaatcg tcgtttctcc tagcgcattt
     3781 tccagccaag tcttcaagta tcgataatcg tctatttcgc catcttcggt taaatctccg
     3841 ctgataatca caaggtccaa gggtgtttgc tgcaggagat gatgaatgca ttgctccaga
     3901 gggataaggg gattctgcat cgtagccagc attcccttat agccttcttc acaagcctca
     3961 tactctctgc gaaagtgtat atctgatata tgcaatatat tcattcttat ctcaatctct
     4021 ttcttataat tccggttatt tgatctaaaa taaacacggt tacgactacg ccaatcaaaa
     4081 taatactcac tttgctccaa gaccgagttt gaatcgcgaa tatcaatgga gcaccgattc
     4141 cgccagcgcc aactaagcct aaggtagccg cactacgaac cgctatttca aaatgattta
     4201 atgctaaaga acaataaatc ggcatcaaat aggggatgcg aacaaagaaa atcgtttgcc
     4261 aaaaatttgc tcctaccgca tgcatttctt caacaagtcg ctcatccatc gcttccattt
     4321 cttctgtaaa aagtttgcca agcattccga tctgatggac gccaatcgcc ataacaccag
     4381 caaaggggcc aggtccgacg actttcacaa aaataattgc aaaaactagt tcaggaaaag
     4441 ctcgtaaaac attacagata agcttgccaa tttttgttac ccatggatgt gcttgccaga
     4501 ggttaaccgc actgatcaag gtaatgggaa tcgctaatac cgtagcgatc attgtcccta
     4561 aacaggcgat tccaatggtt aataatagca aactaatcaa atcctcgccg ctgccatcat
     4621 atagaaactg ccaatcaggc tgacctaacc ctctcaaaac gtccattccc atggaaggag
     4681 agaaggtgct taatccagag taatccagag tagcggccga caaaataacg agaatcacta
     4741 aagcaactaa tgataaaagg gttcttctgt tcatttcacg aagcatcata acagggacct
     4801 cctgacacgt tcacttaatt tatcaacgat cgcgacagtt actaggatcg ttagaataat
     4861 gattgaaact cgttcataat tcaaaagcgc aagtgaggaa tttagaataa cgccaatacc
     4921 accagctccg acatatccaa ggacagtaga agcacgaaca ttcacctcaa acgcgtaaaa
     4981 agcatagctc gcaaactgac tcaaaacttg tggagcgatc gaccaaatag cgatttgcat
     5041 tttatttgct ccaacagccg cagcggcttc aatcgggcca aagtcaatgg tttcaattgc
     5101 ttgaaacatt aattgtgaca ccataccaaa agtaaagaca gcgatagtca acacgccagt
     5161 cgcttcacca attccaaaca cagcaacaaa aagcgcggct aaaagcaggg tagggatggt
     5221 ccgtactaaa cctaaaagaa aacgaaagat ggtagaaaga agacggtttt ctgtcactac
     5281 agtggtcgct aaaaaactaa cagggatcgc taaaattaag ccaatgaccg ttccaagcaa
     5341 agacatttga agtgttttca ccataggctc gactaattta ggtaaatagc taaagtctgg
     5401 atgaatgaag cgtgataaaa agacagtcat ttgattggag ttttgaaaaa cctgagaaaa
     5461 atcagctgac gtgatccagg cacttccttg aaaacaaaca attaagaaca gaagaataag
     5521 caggtgttta taccatttaa agtgaacggt ttctttcaag ctcatcgatt tcgcctgcct
     5581 tttcttcaaa gattgcattg ccataaattt gctctaagcg ttcatcggtt aagtcgtctg
     5641 gacgaccatc aaagacaacc tgagcagctt ttaacgcgag cacacgcgta gaaaactcac
     5701 gagccagagg gactgaatgc agattgacaa tgactgtttt atccatcgtt tgattgatat
     5761 tttttaggtc catcataacc ttttgagtcg tgattggatc taatgatgca acgggttcat
     5821 ccgctaaaat aatgggggct tgctgcacga gtgctcgcgc aatggatact cgctgttgtt
     5881 gcccaccact gagctcatca ctgcgtgtct gcaatttatc agacaaacca actttttcca
     5941 gtgcttcctt tgtctgctgg tagtcttcct tcgtaaaaag acctaaaata cttttccaag
     6001 tggagtaata gcccaaacgt ccagatagca catttttctg aaccgtacta cgctttacca
     6061 gattgaagct ttgcgagatt agaccaatct ggcggcgaat ctgtcgcagc tcttttttat
     6121 tcgcttttgt aatagaggtt ccattaatgc gaatatctcc tttggtggtt tctgccaagc
     6181 gattgatact gcgcagcaga gtgctctttc cagcaccact caaccctatg atggaaacaa
     6241 attctccatc ttcaattgtt aagtcaatat ccttcaagcc aacaacgcca ttgggataga
     6301 ccttcgaaac attgtcaaaa ataatcatta tccttctcca tttttttaag tgtaagcaaa
     6361 aaaaagcttg ctgacactta ctgatgtttt attctgctgc ttttttcgtg tagtctctga
     6421 tcgtgtcata ggcagaatca gcagcttctt catagccctg atgcccccac aaagccattg
     6481 cttctttgcc atctttatca tccttcatat ttaagaaggt ctctttaatt ttcgctttta
     6541 aatcttttga catgtcaggc tgtacagcga ttgcgtcatt cggaatatcg ccttttgtca
     6601 ggtacaggac ttttaaatcc ttaaataaat cattatctgg gaaattcgaa gcaaaaacat
     6661 ttcgcgcgcc ttcaaaaaca aacgccgcat ccatttgctt gttcaacact gcggtaatct
     6721 cactcggaat atcattcaca gtggtcatct ttgtatctgc agtaggatca atcccagcat
     6781 ctttcaattc agcgacagga tagatgtagc cgctagcaga gttaggactt aaggttgcga
     6841 tattttttcc ttttaaatca gttaacttat tcaatccgct atcggcacga accaatactt
     6901 ctcctttaaa ggtgttcgta agtttgtcct ttaatggcaa gccagtttct tgatcataat
     6961 cgccgagctg agaagtcagg attgcatctg cagctccagc atcctttgct tgaacatagg
     7021 ctgcaggcgg cataattccg acatcgactt gaccagaaga catcgcttcg acgatcgttg
     7081 aataatcagt agctaacgtc acttctacct cacgatctaa ctttttcgat aaatacttag
     7141 caaatggttt tgcttttgcc tccatactac catcattgtt tgttggaacg aattgaacaa
     7201 ctaatttttc tttgctctct ttttctgagc tgctacttgt attcccagaa ccgcaggccg
     7261 ccaaagtggt caataacatg actccgatga acccttttgc taactgtttg aactgcattt
     7321 ttctcctcct gttggatata atttctaata cgtctaaatc atagcaggga atcaaaatca
     7381 atgaagtgac gcacgtccta ttttacacaa cttttacata caatttacaa acaattataa
     7441 ataattattc gtattgagcc gaaaaacaat ctaagctata ataaagaaaa acttcttaga
     7501 ggtgacgatt tcatgaaaaa aatccatgat acgaagcaat taactgctta cgttgagaag
     7561 cacaacctgc aggattatat ggattcagat atattggcaa tggcttcttt actttttttt
     7621 gaaaaggaag agcacctcat tcaaacggga aaaacctcag attatttgta ttttttagtg
     7681 gatgggacgg taatggtgta ctcgtattct tctgatacac aaaatatttg catcgattac
     7741 gcaaagcccg ctacaccact cggcgaagcc tcttcattgt gggaattatc gccgaaaagc
     7801 agcgttaaag cagtgactaa gtgcatctgt atcagcattc cgctaaatca atatcgaaca
     7861 cagcttcaaa aagatgttcg ctttttgcag aatatttgcc agttactaag ttatcgttta
     7921 aattccggga taaatttggc gaactcactg acagaacctg ttgaaactcg tttagcaaag
     7981 tttattttga cccatcacga aaacgaacgt ttctcatttc ggttaaccac ctgcgcctca
     8041 atcttaaatg tcagttatcg acatctttta cgaacaatca cgaattttcg tgaggagaat
     8101 attatcgaaa agaaaaagaa ttattacttg attcatgata tcgaagcttt agaggactta
     8161 gctgagaata taagtagtcc agtaaaagca gaataacagc tataaaaaaa cagcagtcag
     8221 aagtgattgc tggttttttg atagtggaga aattgggaaa cggaaaatct tcgttgaaga
     8281 gtagtgttct atatattggt atgctatact tttaaaagtt caatgtgtca agacacataa
     8341 aaaggggaga aaaattttga agaaaatact tgtttcattg ttgtttttgg tgtcattagt
     8401 aacgttagtc ggctgtagtc agtctcaggg ggagaagcaa agtgaaaaag agaaaagtaa
     8461 aatcgctttg gttcttggat ttggcggggt tgatgaccgc tccttcaatc aagctggctg
     8521 ggaaggatta gaggcttggg gaaaagagaa aaagctgaca gcaaaaaaag attttacgta
     8581 tatagaaacg aacaatccag aggataacag taccaatatc gatcaggcaa tcagaaacgg
     8641 ctttaccacc attattagct tgagctactt gcaagcagag gaactggaaa atgctgctaa
     8701 agataatcca aagttgaaat ttgccttggt agatggtgag attactggga aaaagaatat
     8761 ctcatcagca acctttaaag atcaagaaag tgcgtattta gcaggactag ctgccgcata
     8821 tacgacaaaa agtgatcaag taggctttat tggtggaatg gaagggcccg tgatccaacg
     8881 tttccaaagc ggatttgaag ctggcgttaa ggcaggggcc acagcactga acaaggaaat
     8941 tactgttaac gtacaatatg ctgcatccta tgattcgcta gataaaggaa aagctattgc
     9001 tgcttctatg tacaaagagg gagtcgatat tctttatcag gcggcaggaa gtactggagc
     9061 tggggtgttc caagaagcaa aagcccgcaa tgaaaacgac tatagcagaa aagtatgggt
     9121 gattggtgta gatcgcgatc aagcggatga aggggattat aaaatggaga acggcgagac
     9181 agggaatttc acgttgactt ctacaaagaa aaatgtcggt gcagaagtca aagaatttgc
     9241 agaaacttct cataacgata aatttcctgg cggcaagcat ttgacctttg gtttgaaaga
     9301 agaaggggta gatctgacaa aaggacaatt atcagaggaa gcctatcagg cagtttcaga
     9361 cgcaaaagag aaaatcatca gtggtgattt cagactgtag acaaagtcca ttttatggat
     9421 gatgtctaca gt
//
LOCUS       DANKHL010000114         9188 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000114 DANKHL010000000
VERSION     DANKHL010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.118"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            69..2846
                     /locus_tag="PW101_001901"
     CDS             69..2846
                     /locus_tag="PW101_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2514297.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            2927..3346
                     /locus_tag="PW101_001902"
     CDS             2927..3346
                     /locus_tag="PW101_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDL2514298.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            3358..3828
                     /locus_tag="PW101_001903"
     CDS             3358..3828
                     /locus_tag="PW101_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2514299.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            3845..4624
                     /locus_tag="PW101_001904"
     CDS             3845..4624
                     /locus_tag="PW101_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2514300.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            4611..5432
                     /locus_tag="PW101_001905"
     CDS             4611..5432
                     /locus_tag="PW101_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2514301.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            5449..6498
                     /locus_tag="PW101_001906"
     CDS             5449..6498
                     /locus_tag="PW101_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2514302.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            6511..7515
                     /locus_tag="PW101_001907"
     CDS             6511..7515
                     /locus_tag="PW101_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2514303.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8148..9167
                     /locus_tag="PW101_001908"
     CDS             8148..9167
                     /locus_tag="PW101_001908"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL2514304.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3060 a         1538 c         1941 g         2649 t
ORIGIN      
        1 tatatttttc aattctttat taaagatttt attgacaaaa tgaatccgtt ttctatgatg
       61 gaggaggggt gaataagttg ttgaagaagg aaattttatg ttacttagag aaccagacag
      121 cctttttcga tttagaacat atgaacgagg tctttacagc acaaaggtta gctgatcatt
      181 ttggggtaaa aagaaacaca gtcagtcatt atttgaacca gttaaatgat gagcaactac
      241 tagtcaaaat caattctcga ccagtgattt atttccacaa agaagctttt gaaaaacaat
      301 tttttaagtt acgcacgaat ttttattttt ctatcgatga tctcaaagag gaacagcctt
      361 ttttcgcgca accaaaagac ttattctctt ttatgatcgg atacgacgcc agtttgaaag
      421 aaagtatcga acaaatcaaa acagctcttt actatccaga tggcggatta ccacttttga
      481 ttacaggcga aagtggcaca ggtaaaagtt atttagttaa tttggtctat cagtattgtc
      541 tgcttcatga tctgctagaa gattctgcac cattcatcac ttttaattgt gcacaatatg
      601 cagataatcc tgaacttttg actagcaatt tgtttggaca tgttaaggga gcgtacactg
      661 gtgcagaaga aaataaaaaa ggggcttttg aagcagcaga tggtgggatc ttgtttttag
      721 acgaggtaca tcgcttaaca ccagaaggtc aggaaaaact gttcacctat cttgatcaag
      781 gtgtgattta tcgtatggga gaacctaatt tgtcacgccg gatcaaaaca cgtctctttt
      841 ttgctacgac tgaagagatt actagtcatt tcttaacaac tttcattcga agaataccta
      901 tccgaatcga actacctgca cttaatcagc gtagtcgtaa tgaacggcta gaactgattt
      961 actctttttt tctcgaagaa caaagaaaaa tggggagccc tttgtcagtc agtggaccag
     1021 ctttgtcact tttaacaagt cagaaattat ccggaaacat aggagagctg aaaaatatcg
     1081 tcaaagtttc agcagcaaaa gcatatgctg aacagcgtga acagcctgaa atccatgtca
     1141 cgatccatca tttgcaaaaa gaactacttg cgcagcctat tactaaaaat acagcacagc
     1201 aaggaatcta tattacgcaa gaccagacct tagagcaatt aatggagaag caacagcccg
     1261 aacagcagcg aatcgttaaa agttttgagc aaatcctaat tgatttcaag aaaaataact
     1321 gtctgctata ttcttgcgaa ggaaaattaa agcaagaagt gattcaatta tttgattttt
     1381 tgctgtttga gaccagtaga caagaaaaac acgagctgct ggtttattgg acacaatcta
     1441 tccgagagac ctttcgacaa atggaaactg cctatcaaat caaattcgat ggaaacagtg
     1501 tctatgcctt gagttactat ctctatcagc gaagcactat caaatggtta ccggaagaac
     1561 atgatattat ccaactaatc aaagaattag aacaacaagt ttctcagtct tatccatcta
     1621 gttatcacta tgtccaacga attctggaat taagtaagat caaattagat attgaagaca
     1681 caagtatgga tcggatttta ctgacgatct atctaaaaaa agcgaaatgg tcaaaagaaa
     1741 aacgactgcc aaaagcgatg attgctgctc acggttatgc aactgctagc agtatggcaa
     1801 atgtagtaaa tcgtttatta caagaggatc tatttgaatc atttgatatg ccactagatg
     1861 tcacaccaca gcagattgct tcagaaatcc ttgattattg cgaaaacagc gatgtttcaa
     1921 atggattgat catattagta gacatgggat ctctgaaaga aatccatcag ttttttaaaa
     1981 aacagttatc cgtaccgctt ttgattttga ataatgtgac gacaccttta gcaatcacag
     2041 taggggaatg cctgcaaaaa aatgtatcat tagaagagat agcagaagag actgttcgac
     2101 agatccagcc agaatggcga ctgctctatc cagaggaaaa caaacccaaa gcactcatta
     2161 ctacttgctt cactggaatc ggtacggcgg tccacttaag tgaactgcta gaaaaaagct
     2221 tacctacggc ttgtcagttg aaaatcatcc catatgaata tcagcaatta aaagataaga
     2281 aaaacagtga cccattattt tccatatacg aagtcgtagg aatgattggc accacagatc
     2341 ctgatatcac caatatcgac tatctttcat tagaagacct catctcgggg gaaaagatgt
     2401 ctgtactagc tgaatggttg gacactacta tgaatgcttc agagaaggaa atattcaatc
     2461 agcgcatcat tcgtaatttt tcattggaaa aagtactgga ttccgtgact attctagata
     2521 cagagaaggt gatgggagaa atcgatcatt ttatgagaga attagaggtg cagttgaatc
     2581 gaagcatcag caatcccaaa aaattagcgt tgtatgtcca tgtgagctgt ctcattgaac
     2641 gattgatccg acaaatgcca atcgaaacct atcaaggatt agagaaatgg aagcagtgtc
     2701 aaaaagaagg gttatctgct attaaatctg cgtttagtgt cattgaagag cattatagtg
     2761 tcatgatccc caattctgag attgcctata tctatgatat tttgagtgaa gatacagagt
     2821 ttctctcgat agaagaagaa ttttgaacat aagcaagttg ttgccactaa ttttggcatg
     2881 caacttgctt ttttagtatg tgtaagtgaa aagaggagat gaaaaagtgg atagagaaat
     2941 tatcttagct tcccatggaa aatttgcttc agggatatta gattcattag agctgatcta
     3001 tgggaaaaat cattctatta ctgtattcga ttgctatact gatgagaatt ttgatttagc
     3061 ggaaacagta aggcagcttt ttatgaggta caaagataaa gaaatcattg ttctgacgga
     3121 tctttttggc ggaagtgtca acaacgaatt cttacaatat atcaatcggg atcatgtcta
     3181 tctcgttgcc ggattgaatt tgccactatt gattgagcta gtatctcggt tggacgctga
     3241 agtagagaca actgttttga tccagcaggt gctaactgat tcaaaaaaaa tgatccagtt
     3301 ttgtaacgag cggctcaatc agaaaataga agaagacgaa ttttaggagg aagaaaaatg
     3361 attgtattga caagagtcga ccatcggtta ttacacggac aagtagcttt ttcatggaca
     3421 caaacaatag gcgcagactg tattttgatt gctaatgatg atgtacctac gaatgagata
     3481 cgaaagacaa cgatcaaatt agccaagcca caaggagtca aactagtgat caaatcaatt
     3541 gatgactcga ttgccgcgtt gaaaagcggg gtgacagata aatataagtt attcatcgta
     3601 gtggaatcga tcgaggatgc atataagctg gcagaagcgt atccagaaat caaaaccatc
     3661 aacttaggag ggatcaaggc aaaacctggt actcgcagca tcggaaaagc ggtaaatatt
     3721 ttaccagaag aggaggaacg cttgaaacga ttaattgaca aacaaataga agtagaaatc
     3781 cgtcaagtac cagcagataa aaaagtggat gtaagagaag ttttgtaaga aaggaagaac
     3841 ctcaatgatt gtacaagcag tcttattagg aattgttgca tttgtcgcac aatcagaata
     3901 tgcactaggg acatcgttgc tttctcgtcc gattgtgacg gggctgttta caggaatcgt
     3961 gttaggtgat gtaaaaactg ggatcattat gggcgcaact ttagagttag catttatcgg
     4021 atcgttctca gtaggtgctt cgattccacc tgatgttgtg accggtggga ttttagggac
     4081 ggcttttgct attacagctg gagccggtac agaaaccgcg cttttactag gtttgccgat
     4141 tgctacgttg acattgatat tgaaaaatat ttacttaggg cttttgattc caattatgaa
     4201 ccataaagca gacaattatg cagaagaagg aaattataaa ggagtcgagc atatgcactt
     4261 gcttgccggt atcggtcttt ccttgatgct tggtgttgtg gtaatggttt cttatatggt
     4321 gggaagcaat acgatcggaa atctattaaa tatgattcct gacttcgttc aaaaggggtt
     4381 gtcagtagct acagggttga ttccagctct tggttttgcg atgcttgctc gtttgctgat
     4441 caacaaacaa gttgcaccat attttttctt aggttttgct atagcttctt acttagagat
     4501 tcctgtgacc ggagtagcta tttttggtgc gattttagca gttgtggtag tcaatattat
     4561 gaatgtccgt caactaccac ttcaaacaac tgcaggggag gtagaagaag atgaagaatt
     4621 ctgagatcaa aaacagcaaa ataacgaaaa aagagctaaa tcacgtattt tggcgttctt
     4681 ttcaaatgga gttttcttgg aattatgaac gtcagatgaa tatggcttat gtttatgcaa
     4741 tgattccgat tttgaaaaag ttatatcagt cgaaagaaga aatggcagca gctttgaaac
     4801 ggcatctaga gttttttaac acgaccccac atatcgttac actgatttta ggaatcacgg
     4861 ctgcaatgga ggaagaaaat aaggatgatc ctgaatttga tgtgactgcg atcgatagta
     4921 tcaaaacatc tttgatggga cctcttgcag ggttaggaga ttctttcttt tggggaactt
     4981 tgcgattgat tgccacaggt gtgggtacct ctttagccat gcaaggaaat attttaggcc
     5041 ctatcttgtt cattttgatt ttcaatattc cgcacattct gtttcgctat ctagcaacag
     5101 gttggggata tcgcttagga acgggttttt tgaaaaagat ccaggcaaac ggtatgatgg
     5161 aaagtttgac attaggcgcg tctattattg gactgatggt cgttggagca atgactgcca
     5221 cgatgatcac catcaatatt cctttaaaaa tcggttcagg agaaaatgct gtgactgttc
     5281 aaagtatttt tgatgatatc gttccaaata tcttatgtct tggagcattt ggcatagtgt
     5341 tttatctatt gaagaaagaa gtgaaaccat taacgatctt gatcggacta gctttctttg
     5401 gaatttttgg ttcgtggata gggatttttt aaaaaaggag cagagaaaat gtttacgatg
     5461 caagattata tctatgaaga aaaggaagta ctaagtacga tcttgaaaaa gaatgatttt
     5521 tcgacaagag aacatatgaa aaaaacagtg aatttactga tcttagcaac gggttcatct
     5581 tataacgctt gtttagcagc gaagccagca ttggaatctt atggtgatct tactgtcgat
     5641 attcaagaac ctttttattt tttcaattat ggaaaacttt ctccttcaat cgatactgtg
     5701 attgctgtgt cacaaagcgg gaaaagtgct tctactgttg aagcagtaaa aatgatacaa
     5761 aaacaagggt tacctgttgt tgctatcaca aatgacgtgc aaagtccact tgctttagaa
     5821 gctgcacaaa ttattgattt aggtgccggg gtagaatcag taggcttcgt tacaaaaggc
     5881 tattctgcga ctgttttaca gttactactc ttagggatag gaattggaat aagtaaaaat
     5941 aaaatcagca aaaaaataga acaagactac atgcaacagt tgcgcaaaat aatcaaccat
     6001 ttaccagcga ttattcaaaa aacggaagaa ttttttgatg gatatcaaag tttatttcgt
     6061 ttagcacagc gtttcgtcac cattggatat ggtccaaact ggggaacagc taaagaagca
     6121 gagacgaaat tgactgaaac aatccgcgtt ccgtcacaag gatttgagtt agaagcttat
     6181 atgcacggcc catacctgga ggcagatgct tcgcatttgc tattctttat tgaaggggaa
     6241 agtgtaaata aggaacgttc gcaaaagttg caaagatata tgagtcggta cgttggagag
     6301 actctcacca ttaccacaaa aaaagcaaga aacgagaaaa cacttggatt agcaatagaa
     6361 tgtgatgaat atctgtctgt attagcgctt gtcgttccgt ttcaactatt cgcttataaa
     6421 accgcagtag ctaaaggtat tgacttgaac aaaaagattt ttgaagactt tgatacagtc
     6481 ttgaaaagta aattataaga ggtggaaatc atgctgaaat tcaatgaaga agaacaaatc
     6541 aaagcaataa aaggcgcact agctttacgt ccgcaagtag aagaaatcat cgataaactt
     6601 tatgcggaaa aatttgacgc tgtctattat ctaggaatcg gtggaacgta tgcttcctcg
     6661 atgcaagctg tcacttatat gaatgggaaa agcaacttgc ctgtctttgt acagcatgct
     6721 gcagaatatt acactactgg aaacaaaaga ttaacaaaag attctatcgt tattttgtcc
     6781 tctgttaccg gtacaactca agaagtagtg aaagcagtag aacaaatcaa gaaagtagga
     6841 gcgacactga ttggatttat cgataaagcg aacagtaagt tgtctcagct atgcgatttt
     6901 gtggtcactt atccagctcc tggaacagaa caaatcaaat tttttatggc agcagatcga
     6961 ttgatgtatt tgaatggaga attcgaagct tattcagaat actatgaaca gctcgaacgt
     7021 tatttaccaa cagggttagt ggaagcagag aaaaaagcag atgcatttgg cttatcattt
     7081 gcagaaaaac atcgacgtga tagtatgcat tattttattg gagcagggaa tcaatgggga
     7141 gctgtttatt catatgccat gtgttactgg gaagaacaaa gctggcttcc atcgaaatct
     7201 atccatgctg ccgagtttct tcacgggacg ttagagatcg tagaagaaac aaccccagtc
     7261 acattatttt taggcgaaga tgaacagaga gtgttatctg aacgtgtggc taaacttttg
     7321 ccaaaaattt gcagcaatta tacattgata gacacaaaag actatccagt agaaggtatc
     7381 agtgaaaaat atcgcggacg tgttttgtct ttcttattga tgcatgtagt gacacaacgc
     7441 atcgatgctc atgtggagca gttgaactgc catccattag agatacgccg ctactatcgt
     7501 cagtttgatt attaggatag actggataaa aagaatataa aaaataaaga gagcagcgat
     7561 aactgctccc tttatttttt taaaatattg gcatcaatta cgttataaaa cacatttttt
     7621 gcattatcta cagttcaact tataatctgt ttaatgggac atgtgggtgc caaccatttt
     7681 ttgttatgca atagtactat ttagttgttg catggatccg tagtaaaagt ccaagtacaa
     7741 cgagttgcgc cagtttacat agcgttttgt atccagaatg ttgactaaaa caaaagttcc
     7801 tatgtcaacc gtcaacagag acaatctgtc cgttgcagga cctgacagat ttagaaatgt
     7861 tcaatctgtc tcttatgtat tttaaatcaa tactcactag gagaagtttc ctaacctcct
     7921 aatagtaatg ctggtagtaa cattgtcaaa actgcttcct cttaggaaca agcaaagcat
     7981 aacgagtgta aaaataaaat gtagactatt gtaacatatc attgattatt tcaagtaaaa
     8041 acgataaaag aagtaaacaa acacttaaaa acaatggaaa ttagtcccaa ctttaagaaa
     8101 tgtatactat aatggaaatt atcaattatt tatttggagg aatatccatg aaacaaaaaa
     8161 tcgcagttct tggtcctgga tcttggggca ctgctttagc acaaacattg gcagaaaacg
     8221 ggcatgatgt ccgtatttgg ggaaatgtac ctgaacagat tgatgaaatc aataagtatc
     8281 atacaaatca gcatttttta cctgatttga cgatcccaga atcgattatc ggatataaag
     8341 aattggcaga agcagtggat aacgcagatg ctattttatt tgttgttcca acaaaagcga
     8401 ttcgttcagt cgcacaggaa cttattggta aaatgaatac taagccaatc attatacatg
     8461 caagtaaagg actagaacaa gatacgcata aacgaatctc agaagttttg gctgaagaaa
     8521 ttccagaaga aaaaagacag gcaattgttg tattatctgg tcctagccat gctgaggaag
     8581 tggcagtaca tgatatcaca acaattacag ctgcaagtat tgatcaaaaa gcagcagcct
     8641 atgtacaaaa actgttcatg aatgattatt tcaggattta tacaaataat gatgtgattg
     8701 gcgtagaaac tggtgcagca ctgaaaaata tcattgcaat tggtgccggc gctatccatg
     8761 gattaggatt cggagatgat gcaaaagcag caatcatgac tcgaggattg gcagaaatca
     8821 gccgtcttgg tgtcgcgatg ggtgccaatc ctctgacatt tatcggtttg agtggtgtgg
     8881 gggacttgat cgttacttgt acaagtgttc attctcgcaa ttggcgtgcc ggtaacttgt
     8941 taggacaggg acataagctg gaagaagttc tagaaaacat gggaatggtc gttgagggtg
     9001 tggcaacgac aaaagcagca gtagagctag ctcaacagct aggtgttgag atgccaatta
     9061 ctcaaacaat ctataatgtt ttgtataatg gagaagatat caaaaaagca gcaaaagaaa
     9121 tcatgttacg tgacggaaaa atggagaatg aattttctct tcgttaaata gatgatcaat
     9181 agttaatc
//
LOCUS       DANKHL010000115         8962 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000115 DANKHL010000000
VERSION     DANKHL010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.119"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            278..786
                     /locus_tag="PW101_001909"
                     /pseudo
     CDS             278..786
                     /locus_tag="PW101_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
     gene            879..2041
                     /locus_tag="PW101_001910"
     CDS             join(879..1155,1155..2041)
                     /locus_tag="PW101_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2514305.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2321..3301
                     /locus_tag="PW101_001911"
     CDS             2321..3301
                     /locus_tag="PW101_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2514306.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3423..4841
                     /locus_tag="PW101_001912"
     CDS             3423..4841
                     /locus_tag="PW101_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HDL2514307.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4912..6381
                     /locus_tag="PW101_001913"
     CDS             4912..6381
                     /locus_tag="PW101_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2514308.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6391..6858
                     /locus_tag="PW101_001914"
     CDS             6391..6858
                     /locus_tag="PW101_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2514309.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6894..7766
                     /locus_tag="PW101_001915"
     CDS             6894..7766
                     /locus_tag="PW101_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2514310.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7847..>8962
                     /locus_tag="PW101_001916"
     CDS             7847..>8962
                     /locus_tag="PW101_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2514311.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQL"
BASE COUNT         3087 a         1332 c         1799 g         2744 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaggaaata agaaaactaa acttgtatcg
      601 ttaacaaaat atggtcgaaa ggtgtacgaa tcacataatt atttacatga agagctttat
      661 agctcaataa atgaagcgtt agaatcattc tcaccagacc aaatttcaac cattaaacag
      721 attctagaaa aagtatctta tacaatcaat gaatatcaag taagtctgac aagaaaggaa
      781 gattgacatg aaaaaaatga tttttgaatc ggctacacta gactagacag aaaaaataag
      841 gtgtgtaaaa taaatgaaaa cacactggag gaatttttat gtcaagacgt caacgtagaa
      901 cctattcaaa agaattcaaa caacaagtcg tcgatcttta tctcgcagga aagccccgag
      961 ctgaaatcat tcgagattat gagctgacta cctcggcctt tgataagtgg gtcaagcaag
     1021 cgcaaactac tggctcgttt caggaaaaag ataacttgat ccctgaacaa gtagaactaa
     1081 ttgcattacg aaagaaaaat aaacagcttg aaatggagaa tgacatttta aagcaagcgg
     1141 cgctgatatt gggacgaaaa gacaagtaat tgataccaat aagcacaaat actcaatatc
     1201 agcgatgtgt aaagtgttaa acatctcttg ccaaacctat tattatcaag ccaaaccagt
     1261 tgaagatgaa tcagaactgg aagaactcgt tcaagaagaa tttattcgca atcgaaaagc
     1321 ttatggtacc aggaagctga agcagtgctt ggccaatcta ggtcttcaag tgagtcaacg
     1381 cagaatcggc cgtatgatga aacagcgtgg cttgcgttca acatacacat tggctcattt
     1441 caaattccat tcttctactt gtaacgaagc caagaccgcc aatattctag acggaacctt
     1501 cctacaggaa aagtcattag aagcgattgt aaccgacctt acctatgttc gtgttgggaa
     1561 gaaatggcag tatatctgcc tgattcttga tttgtttaat cgagaaatca tcggctattc
     1621 atgcggggag aagaaagatg cgacgttagt caaggaagct tttgcaaaga ttccgtactc
     1681 gttgaataat gtacaacttt ttcacacgga tcgtggaaaa gaattcgata accagacgat
     1741 tgacgaaatt ttatgggcct ttaatatcac gcgttcctta agcaaaaaag gctgccccta
     1801 tgataacgcc gttgcagaat ccacctataa atctgtcaaa gtagaattcg ttcatcaata
     1861 ccgctttgag acattggcta atttgcgatt agagctattt gattacgtcc attggtggaa
     1921 taccatccgg ctgcacggca cgttaggcta tgagacaccg atcagttttc gacagcagag
     1981 attggcgcag cgaatccttg ataatgagct cggatgtgat accggtggcg aggcacctta
     2041 attatttgaa tgagcttctg ccttagagaa tcagattcga ggaggctcgt tgtcaagggc
     2101 agtcggaaca ccagcagcaa gcttacgcaa taccttataa tttttgtcaa aaaaagtgtt
     2161 gccattccac atatatcttt aaacagctat tgccagataa aaataaatga tattatatat
     2221 ataaatactt atccgaaaag gggaatgaga gagtcaaggt tcttggaatg tcgaatagtc
     2281 gagtaggtag attttctatc tacaaccttg ttgtttaaaa atgaaaaaaa ttggaattag
     2341 agaagttgcg aaagaggcaa atgtttctcc tacgactgtg tcgagggtat tgaatgatcg
     2401 tggatatttg agtgaggaaa caaaaagaaa agtttttgat gccatgaaaa aattagaata
     2461 ctatcctaat gaactggcga gagcactttt taaaaacgaa acatataccg taggtttgat
     2521 ttttcctaca attactaatc catttcacgc tgagttaatt caagatatcg agttcgagct
     2581 ttccagcagg ggatataaag tgttgctgtg caatagctta aacaatccaa caaaagaaaa
     2641 agagtacttg acgatgttga gaaagaatca ggttgatgga attatagtag gaacgcacaa
     2701 taaagatatt gcggattatg atattccagg gctacctatt gtagcgattg atagaagtct
     2761 tggtgaaaat acagttacag tatcttgtga taattacagg ggtggggaat tagctactca
     2821 actacttgta aatcatggct gtcagaatat actgtgtatt cggggagata gtaagataaa
     2881 gctacccgcg aatagtagga ctttagcata taaagatgtt ttgaataaat tcatgctgga
     2941 accgttagtc ctagaagtac catttgtaaa agaaatcaat gaaaaaaatg aaataattaa
     3001 gacatatctg gaaactcatc caaatattga cggggttttt gcaggagatg atttattggc
     3061 ttctttggcg attaacaact taggaagttc gggcaaaaga gttccaaaag aggttaaagt
     3121 gattggattt gatggagctc aacaaacatt gatttataat ccagaattat caactattca
     3181 acagcctata gatcaaatat ctaaagtagc agtaaaaaaa ttattgaata tgattaaggg
     3241 cgaaaaggag acggatgaac tttcacttcc agtaaagctc ataaaaaaaa atacagcata
     3301 agttaaatcg gcgaaaaatt ccttcgtcga tttaaaaata tttttatgaa acgggttgac
     3361 atatgaaacg ggttgacata tattagttgt gaagataaaa accttttcac tggaggaaga
     3421 aaatggagaa attattaatt aaaagcaata atcatcaact ttactaccaa cctaaggatg
     3481 tttgggtcgg agatgttatg ccttacggga aagaaggaaa attctttatt tatcatcaac
     3541 gtgatactag aaatccagtt ccttttggag aaccgtttgg ctgggcttta gcaactacga
     3601 aagattttgt tcattttgaa gactttgggg aatctctttt aagaggggat gacaatgaga
     3661 gagaccagtt tatctacgct ggttctgtat ttgaggcaaa tggaaaggtt cacgcatttt
     3721 atacagggta taatcgtgaa ttcttagcgc agggaaagac atctcaagtg ttgcttcatg
     3781 ctgagagtga tgattttgtt cattgggaaa aatcagaaac agcattggag ttagaaccac
     3841 aagaagggta tgatattcga aactggagag atccatttgt cgtttgggat gatgaaaaag
     3901 aagaatattt gctaatttta ggggcgcgaa agggtcagga taaacatcta caaaccggac
     3961 gtttagtgga atttacatca aaagatttaa ctaattggga attcaaaggt gatttttggg
     4021 ctccgggatt gtataccatg tttgaaatgc cagaactttt caagattggt gactggtggt
     4081 atcttgtcta ttcagaatac tcagatggaa ataaaatact ctatcgaatg agtcaaagtt
     4141 tggaaggtcc ttggattaag cctaaagatg aagcttttga tggacgggct tattatgcag
     4201 gtagaacagc ttatgatgga aaacgtcgag tcctatttgg atgggtacca acaaaagatc
     4261 aagaaaatga taaaaacaat tatttatggg gaggtacttt ggttcctcat gaagtctatc
     4321 aaaaagagga tttttcactt ggggtaaaac ccatagacga agtgtggaat gcattcataa
     4381 atttacatga tattgccgat gtagagatag aaacagcaga cacaagagaa gaagtaacgc
     4441 ttgtcaaaaa tagtggaact ttgtttaatt tagaaacaac aatttcattt gatgagaatc
     4501 ttagtgactt ttcactgaga ttatataaaa accttgagac agatgaatct tacgaatatc
     4561 gcttcaattt agatgagggg cgtcttacat ttgataaaaa cccttgttac ccttggtttc
     4621 aatatatgga taaaggactt attagaccta tatctttaaa gcctaaccaa tcttataaac
     4681 taaaagtgat tgttgatgac aatataatga cactttatat tgatggaatc gctcttaacg
     4741 ctcgtgtaca tggttgtttt ggtcaagcaa ttactattac agcaacaaat ggatcaatga
     4801 ctgcaataaa taccaagttt tcaaatgaaa taaagaaata ataaacgtca atcatgtggc
     4861 aggtctgaaa atcaaacatg acctgtcaca tttaaaaggg aggataaatg aatggactac
     4921 aaaaaaattg ccgaagatgt attggaaaac gttggaggaa aagagaatat ctcaagcgct
     4981 acacactgca ttactcgttt acgtttaatt cttaaagaca aaagcaaagc gaatatggaa
     5041 atgcttgagg aaatagaagg tgcaaaaggt gttcttttca atagtggaca gttacaagta
     5101 attttcggaa caggtataat tgaaaaggtt tacaaagagt tcgttgaatt gacaggagct
     5161 aaggaagtat ccgtttctga aatgaaagac gaaggagtca ataattcagg aaatagactt
     5221 caacgagctt ttaaagtttt ctcggatatc tttattccga tcattccagc ttttattggg
     5281 gcagcaatgg ttttaggaat tcgttcgttg attaccactc ctggaatgtt tggaatggat
     5341 ggctcattcg ctgatcacaa tgtattccta aatgatttat ccaaatttct gggtattatt
     5401 gcaaccacat ttgattattt accagtttta gttatgtatt ctgcaacaaa acgttttggc
     5461 ggaaacccta ttttaggatt acttgtagga tttgttatga ttcatccagg actagccaat
     5521 cggaatgatt tcgtcatggg aactataaag cctgattatt ggcatttatt tggattaagc
     5581 attccagcag ttgcgtttca agggggtgtt tttccagcaa ttttgacttc ttggttttta
     5641 gcaaaagttg agaaaattac aaataaatat tctcctcaag tacttgctta cattttagtc
     5701 ccaactgtga caattgttct tgccaacttt gcactatttt tagtgtttgg tccattgggg
     5761 aattttatcg gtaatgtttt aggtggaatc attgatttcc tatatatgaa gatgggtgcc
     5821 tttggtgctt ttgtttttgc aggcgcgtta caaccattag ttgtaacagg aacacaacat
     5881 gctattcaag gtattgaggc aaacttagtc gcaacaactg gattcaatta tatccaacct
     5941 atttggtcag tttcaattat tgcccaaggt ggcggttgta ttggtatgta tttactcttt
     6001 aagaaaaaat ctaaagatag agatattgcg atttcaagct ttattccaac actggtaggt
     6061 attactgaac cggctatctt tgctgttaat ttgaaatatt caatcgttcc ttttgtttgc
     6121 tcgtttattg gtgctggttt tggtggagca tttatgaaaa tttttgatgt aaaagccatt
     6181 ggacaaggat tgactgtatt gccaggtctg actattgcta atccagttct tccttatata
     6241 tttggtaatc tcatagcatt cattttacca atcattttta ttctcgctta tgataaatat
     6301 aaagtgttta tccctgaagc taaacaccgt aaatttgcag aaaaaactga ctcaaaaaaa
     6361 ttgccagttc aagtaaagtg agatgagagc atgttaaatt tttttagaaa aaatgaaata
     6421 agcgttgtag cacctgcaac cggtgttttt gaaaaactgg agaaattaag tgatccagtc
     6481 ttttcaaaag gaatgatggg acaaggattt gcaattgatg caaaaaacga aacgattgta
     6541 tctccaatcg atggttttgt ttcaagtatt tttcctacaa aacatgcctt aggaataaaa
     6601 acaaaatcag gaattgagat acttgttcat gtaggaatag atacagttga gctaaatggt
     6661 gagggatttg atattaaagt tcaagaagga caaaaagtta aggctgggga tattcttatg
     6721 accattaatt ttgtagtttt tgaaaagaat cacaagacta aagatgtcat tgttgttttt
     6781 ccagactacc aagacgaaat tttcattccg aaacttgaag aagtaaaaca aggacaagaa
     6841 ttgtttgttc ttggctaatg ataagtaatt aataatagaa tcgaggaaaa tgtatggttt
     6901 ttggggctat tgaagctggt ggaacaaaat ttgtttgtgc cattggagat aaagatttga
     6961 acatatttga aaaagtcagc tttcccacga ctacacccaa agaaacaatg aatttagttt
     7021 tagatttttt taatcatcat aaagatgatt tagaaggtat cggagtggga tcgttcggtc
     7081 ctattgatat tcattgtgaa tctaaaacgt atggcttcat taaatcaacg ccaaaacttg
     7141 cttggcagga ttttgatttt attggaagtt taaaaaagca acttaatatt ccaactgctt
     7201 ggacgactga tgtaaatgcc tcttgctatg gtgaatatgt ttctggaaat ggcagaggcc
     7261 tttcaagtgt tgtttactat accgtaggaa cgggcatagg aggtggcgct atccaaaacg
     7321 gaaacttcat tgaaggtttt agtcatccag aaatgggaca tatgcttgta aaacgacaca
     7381 ataaagacaa cttttctggg acatgtccat tccacggaga ctgtttagag ggcttagctg
     7441 ctggtccggc aatcgaaaga agatcaggga aaaagggaca agaagtgact gaaaatgacg
     7501 ttacttggga aatagaagct tattacattg ctcagtgtgc atataataca acattgatgt
     7561 tatcacctga tgtcattata tttggtggcg gagtaatgaa acaaaaacac ttaaaaaaga
     7621 aagtacaaga taagtttatt gagttgatca acggatatgt cgctattccc gatgttgaca
     7681 attatattat gacaccaaaa ttgttagata attctggtat tgtaggttgt ttagctttag
     7741 caagagacgt taaagaaaaa tcttaatttg gaaaaaagat cgtctcgact atttgtttgg
     7801 ttgatgcgat ctttttttca attagtaatt gaagggacgc ctaaaagtga aaaaaaaaat
     7861 gaaagatgaa gtattagagt atatcaaaaa taatttaaag tattacgatt tttctgctca
     7921 agatattgca atgaaatttt gtattaaaag aaatgtcgct tcccactatt tgaatcagtt
     7981 attttcagat ggaaaattac taaagaatga ttctgttaga ccagtaatgt tcaagtataa
     8041 tcaacaaaag ccaaaagact gcttttctaa gtttattgga gcagacatat ctttgaaatc
     8101 cactatagat aaatgcaaag ctactgtaat gtatccccct aatggcctcc cattaataat
     8161 aaaaggaaac agtggcgttg gaaaaagttt tttagcaagt ttgatctatc agtatgctct
     8221 tgatagaaaa gtaattcata atgacgcaaa atttgttgtt gtgaattgtg cagattatgc
     8281 aaataatcca gagctattgt cagcagtttt atttggatat aaaaaaggag cctttactgg
     8341 ggctgaaaaa gatactgcag ggatgctatc ttctgctaat ggaggatacc tttttttaga
     8401 tgaggttcac aatctaagtg cagaaaacca agagaaattg tttcttctta tggattctca
     8461 aaagtatcgt aaacttggtg aaagtgtgca atgggagtat gcaaatgttc gccttatctt
     8521 agccacaaca gaagataaaa atacctcttt gttagcaact tttagaagac gcatcccagc
     8581 tgaaatcact ctgccagatt acaataatag aagtacatct gaaaaaattc aattattatt
     8641 tgaatttctt aaagatgaag caattacgat tgattcaaaa atattttgtt cgattgaact
     8701 ttttactgat cttttatcga aaacatttga aggaaatgtt ggagaattga ggaatgaaat
     8761 caagcttctt tgtgcagaag gttatttagg aaataagaga aagtcttcaa tttatttagg
     8821 ggacaaacta gacagcggtt tttggataga tccagaaatt agtatagatt taaaaaaaat
     8881 gtttcttaaa tcactttctg aaattgattt gattgaactt tttcaaaatc acatttcaat
     8941 tgaagaaagc atgagtcaat ta
//
LOCUS       DANKHL010000116         8875 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000116 DANKHL010000000
VERSION     DANKHL010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.120"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(35..703)
                     /locus_tag="PW101_001917"
     CDS             complement(35..703)
                     /locus_tag="PW101_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2514312.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            837..1124
                     /locus_tag="PW101_001918"
     CDS             837..1124
                     /locus_tag="PW101_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514313.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1540..3078
                     /locus_tag="PW101_001919"
     CDS             1540..3078
                     /locus_tag="PW101_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDL2514314.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3075..4040
                     /locus_tag="PW101_001920"
     CDS             3075..4040
                     /locus_tag="PW101_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2514315.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4027..5349
                     /locus_tag="PW101_001921"
     CDS             4027..5349
                     /locus_tag="PW101_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2514316.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5412..5516
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5590..6333
                     /locus_tag="PW101_001922"
     CDS             5590..6333
                     /locus_tag="PW101_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2514317.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6348..7340
                     /locus_tag="PW101_001923"
     CDS             6348..7340
                     /locus_tag="PW101_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2514318.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7352..8089
                     /locus_tag="PW101_001924"
     CDS             7352..8089
                     /locus_tag="PW101_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2514319.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8079..8801
                     /locus_tag="PW101_001925"
     CDS             8079..8801
                     /locus_tag="PW101_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL2514320.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT         2959 a         1515 c         1862 g         2539 t
ORIGIN      
        1 tctgtaaatt aaataaagta gtgaaaaata agaatcagtg ctctaactgt tcaagaggat
       61 caagtggttc aaatgtttct gtagtctgtt ttgcttccaa ttggggcaaa acagatccaa
      121 tttccagata cttcacttta ttcaatgtat aagatagaat agaacagcta agtaatgtat
      181 acagaaaatg tgtgagatcc aaataaagca aggagacaac taaaatacca aaagtaatca
      241 agaaaagact tttgccaatc ttaatatgaa aatatttttc aatggcttga taaatgatat
      301 ctgttccgcc tgtgcttccg ccgaatcgaa agacgagacc gcaaccaatg ccagaaagaa
      361 tacctgtcat caaactattg atcagtagac tatctacagc aaaatgtata tgaaggctct
      421 cccataaatg taaaaagaaa actagcgcaa atgttccgac gatcgtccgg agaaaagcgt
      481 gttttccaaa aagaaacaat gagataagca ataaaggtaa attaaaaagc agtgaagtga
      541 cagaaggagc gaatccaaaa agattatgca aaagcaaagc aataccagta accccaccat
      601 ctgccagctt agatggaata ttgattcctg tcacagctaa tccgtagatt cctgcgccta
      661 aaatgattcc gcacaattct gtaaataaat ttaatttttt catttctagc ctcttttctt
      721 ttgaaaattt tcagcaactt ctttattata ctgacttata ctcttacttc aagaggaaaa
      781 tgaaaaaaaa ttatttttgg ctgtttcttt ttatttatca aataaggagt ggaaaaatgc
      841 gagcagcaga cttattgaca attcttacaa aatcacccaa aaaaacagag tatcaaggag
      901 tttttttggc tcaagaacaa aaactggtgc ctgtgacaat ggtccagata aataaagcaa
      961 atcaatttat ctttttattt gaacacggca aagcaccact tgctatgaaa gacatctttc
     1021 tttttttgat gaagaaccgt gagaaagctg tttattatca gtcaaaagaa gaactcattc
     1081 ctctttatgg agtgaaagag atagaaaata aagtggtgat ataagtaaaa ataagaaaac
     1141 aagacgttta tgatgtcaaa acaaccattt tttacatttt tggcaaaaaa gtgtcatgat
     1201 acagtataat attcgttgaa caggccaata aaggtgttct cccccacgag atttctactt
     1261 tattggctgt ttctttttat taaaataaaa gttgtgtgtg taaaaaagat atagctgatt
     1321 ccaagtgaca atttcgtata gatttttatg gtattcgtag tgaaaaaacg agaaccaaaa
     1381 agttgatccg actttgttgc ttggattctt tttttgtcag catatttaga ggtatttttt
     1441 tctttttttt gttcgtgctg taaggttaag gcaagacaag ttagttggga aaaaggaaag
     1501 aaatgtttat acttaaagaa taacagaaag gaaggtcgca tgaataattt tatacaaaca
     1561 ttaagtgaaa gaaaaggcga gttgctggaa gctgcatttc aacatctgtc catttctctc
     1621 atttctttgc tgattgcagc actgattgct attcctttag cgatttgggc agccaatcat
     1681 aaaaaaatgg cagaagtctt attgcagatc acgggtgtgc tccaaacgat tccttcactt
     1741 gccttattag gtttacttat cccatttgta gggatcggaa ctgttccagc attgattgca
     1801 cttgttattt acgctttgct tccgattttc caaaacacgt atattggtct agctgaaatt
     1861 gacccgtcta ttgaagaagc agcggtagca tttggtatgt ccagaatgag acgcttgctg
     1921 aaagtagagc ttcccatcgc attacctgtc attatctcag gtatccgaac tgccttagtt
     1981 ttaatcatcg gtacggctac tttggcagca ttgatcggag caggtggatt agggacattt
     2041 attttgttag gtattgaccg aaatacacct gttttgacat tgattggtgc gatcagttct
     2101 gccttgcttg caattatatt cagcggactg attcgctggt tgcagcataa gaaacctcga
     2161 tattccatca ttactttagc agcagtggtg tgtttatttg gcggcgtttc tttatatcag
     2221 tctgccttgt ttaaaaatga aaccatcacg attgctggaa aattaggagc ggaacctgaa
     2281 atcctgatcc aaatgtataa agaattgatt gaagacaaca caaaaacaga agttgaatta
     2341 aaaccgaatt ttggcaagac gagttttttg ttcaatgcac tggatcaagg tcaaatcgat
     2401 atctatcctg agttcacagg tactgtccta gaaagcttgg tgaaggtacc tgaagcaatg
     2461 aaaagtgctg atttgacaaa agaaaaaaca tatgacgaag cagaagaatt attgaataag
     2521 caattcgata tgacattatt aaaaccaatg gcttaccaaa atacgtatgc cttggcagtc
     2581 aagagatctt ttgcgaacga acatcaactt aaaaagattt ccgatttggc agctatctct
     2641 gatcagatca aagcgggatt cacactggaa tttattgatc gttcagatgg atataaagga
     2701 atccaagaag catatggctt gaatttttca agtgtacaaa gcatggagcc aagtttgcgt
     2761 taccaagcaa tcaataatgg agatgtcaat attgtggatg cctattccac agatagccag
     2821 ctcaaggagt acgatctagt gactctagaa gatgatcgag ggttattccc agcttatcaa
     2881 ggcgcaccgc tgatgaaaaa agattttgct aaagaacatc ctgaaatcgt taaagcactg
     2941 aataaattaa gcggaaaaat aacagaggat caaatgattg atatgaatta ccaagtcaat
     3001 gtcgaaaaga aaaagccgac agaagtagca cgaatcttcc tgaaaaatga aggattagta
     3061 aaggaggaag atcagtgact aaattaatcg agttcaaaca tgtgcaaaaa aagtatgatg
     3121 gaaagtatgt catcgatgac ctaaatctgg caatcaaaaa aggagaaatc tttgttttgg
     3181 ttggtccttc cggtagtgga aaaacaacta ctttgaaaat gatcaatgga ttatctaaac
     3241 catcagcagg agacatctac ttcaaaggaa aatcgttaaa tgaatataat ctgcagaaaa
     3301 tgcggtggaa tatgggatat gtgctgcagc aaatcgcttt gttcccgacc atgacagtca
     3361 agcaaaatat tgaagtcatc cctgaaatgt taggttggga aaagcagaaa agagcagacc
     3421 gtgtagatga acttcttcaa aaagttggct tatctcctga tatttatcga gatcgtatgc
     3481 ctagagagtt gtctggagga gaacagcaac ggatcgggat catccgagct attgctgcaa
     3541 gcccagatgt gattttgatg gacgagccat ttagcgcatt agatccgatt tcaagaaact
     3601 cattacaaga attggttttg tccttacacg aagaattggg cacgaccatc gttttcgtta
     3661 cgcacaatat ggaagaagca atcaagctag gtgatcgtat tgcatttatg aaggatggag
     3721 aaattatcca atgtgacaca ccagagcagt tattgatgaa tcctaaaaac gactacgttc
     3781 ggcatttctt tgatgaaccg aaacagacga aagagtggcg agtagaagat ttagtcgtca
     3841 atggatattt cttaaatgaa ataccagaaa atgctcgtca acaagtttgc tttgatacgc
     3901 ctatgaaaga agtatattcg cttctttcgg tgtatccaag cattgtgata gtagaaaagc
     3961 aaagaagtat tgggtcgctg acaagtaaag aaatctttgc ttttttaagt cgagaggagg
     4021 aaggaaatga aaacatatga tgcaatcatc atcggttcag gtgtgagcgg cttaagtgct
     4081 gcttatggat tgaaagaagc aggtaaaaca gtactagtgg tcgaagaaga tctatggggc
     4141 gggacttgtc ctaaccgtgg atgtgatcct aaaaaagtct tgttaagtgc agtagaggca
     4201 cgtaatcggg taaaacaatt gagtggaaaa ggttttaacg agatcccaac tgccaactgg
     4261 gaagaacttc aaaaattcaa acgaacattt acggatccag tcccagaaag ccgaaaaaaa
     4321 caattagcag aagctgaaat cgatcatctt tcaggaaccg ctcgtttttt ggatgattca
     4381 tctattgaag tcaacgaaga agtcttccat gcggattact tagttttggc aaccgggcaa
     4441 agacctacga tcttaccagt ggagggaaag gaatacttga aaactagtgc agacttttta
     4501 tcattgcctg tactgcctaa agaaatcatt tttatcggtg gtggttatat tgcttttgag
     4561 ctagctacga ttgccaatgc tgcagggagc aaagtaacca ttgtccatca taatcaacgg
     4621 ccattaaaag aatttgaggc gtctttggta gaggaagccg tccatcaaat ggaagcatct
     4681 ggcattcagt ttgcttttgg tgttgaaacc caaaagatca tttcagaagg tactcgctat
     4741 cgtttagtcg gaaaagagac ggaattagta gcagatatga ttttttgtgc gactgggaga
     4801 cagccgaata cagaatcttt agcacttgag caagcgaata tcgtctttga taaacacgga
     4861 attgcagtca atgattatct gcaaacgagc aatccaaaaa tttttgcgtg tggagatatt
     4921 gtttcaagga aaaccccaaa attgacacca gtcgccactt ttgaaggaaa ttatgtagct
     4981 aagcggataa cggatgcaac aagcgaacct atcaaatatc cgattatacc tacgatcgtc
     5041 tacgccagtc ctaaacttgc tgaagtagga gttacaaaaa gtcacgcctc ttcgtcagat
     5101 caagtggtag agatggatct taccagttgg ttcacctatc atcgtgtcaa tgaaccagtt
     5161 gctaaggctg aattgacctt tgaccagcaa aattatttga ttggtgctgc agtgattagc
     5221 gagcaagcag acgaattgat cgatgatttg acactcgtca tcaatcaaaa attgactaaa
     5281 aaagagttgg atagctatat catggggtat ccgacattgg caagtgatct ctcctattta
     5341 ttgaaataat cagcatgata actaaagcgc ttgttgcttt ttccttcttg tgctacactt
     5401 taaaaaatga atacctgttt ctggggatgt cgtagacgac atgagagtaa gttgtgaact
     5461 tagacccatt gaacctgtca gttaatactg gtgaagggag aaacatagca gacgcttgaa
     5521 gcgggttact atgtcgtgcc catttcaagc gttttttgtt attcatatta gaagaaagga
     5581 ggtaacaaaa tgaagaaaaa tctttatcca gcagtcagta tggcattcat tttaattatt
     5641 tggcagattg ttgtcatagt ttggaaaata cctgccttta ttttacccgg acctgctgct
     5701 gtattttctg cattagcggc agatgcacaa gtattacttt cccattctgt cacgacatta
     5761 aatgaagcag tgcttggttt ggtaatagct agcgttcttt cttttattac tgccttggca
     5821 atggattatt ggaagttttt agaaatgtct ttttatccgt tactagtgat ttcacaaatg
     5881 ttgccaatca tggtgttagg gccattactt accctatggt ttggtttcgg tatgcttcca
     5941 aaagtgatcc ttgttgtctt gataagctat tttccgatcg tagtttcttt ttcggataca
     6001 ttgaggaaag tatcaaaaga gcaaatcatc tttttgaaaa cgatgggggc agatacaggg
     6061 aagatttatc gaatatataa aataccagcg ggtttatccg gcttcttttc cggtttaaaa
     6121 gtggctgcta cttattgtgt cagcggagca gtcgttggag aatggctaag cgctcaagca
     6181 ggcttagggt attacatgat acgcgtaaaa aacagctacc agatcgataa agtttttgct
     6241 gcgatcattt gtgtcattct attgagttta ttgctgaatg gcgcttgttc attattgaaa
     6301 aaagggtatt atcgtatgct ttatattttt taagagaagg ggatcaaatg aaaaaattat
     6361 ggcttttatt accgctactg ttgcttttat ccgcttgtgg cactgcaaaa gaaacaagta
     6421 gcaaacaaga aataaaggat ttgaaaaaag tgacacttgt tttggattat gtaccgaata
     6481 ctaatcatac tggcatctac ctagcaaaag aaaaaggcta ttataaagag gctggtttaa
     6541 atgtccaaat catcgaacca ggagataatt cgacaagtat tggtctagta ggtgcggaca
     6601 aagcacaatt tggtgtaagt tatcaagaag atgtcactta tgctcacgca gatggacaga
     6661 atataccagt caaagcaatt gctacagtga tcaaacataa tacttctgga ttcgccaccc
     6721 tatcagacag taacatccat tcaccaaaag attttgaagg aaaaacatat gcaggttggc
     6781 aaagtccaag tgaagaagct gtcttaaaag ctgtgatgga aaaagacggc ggggactttt
     6841 caaaactgac aatggtagga agcaatggag aagggccaga aagcttagga aaatcctcag
     6901 atatccaatg gtactttgaa ggttgggata tgatcaaagc aaaagaagcg ggtattgaac
     6961 taaactatat cccattgaaa gaattagatg aacggttaga ttactatact ccggtaatta
     7021 ttacgaatga tcaactgatc aaaagtgatc cagaattggt acagtctttt atggatgcca
     7081 cgaaaaaagg ctatcaggaa gctattaagg atccaaatga cagtgctaaa ctgttgcaaa
     7141 aatatgcaaa agaaaatgat cggacgtttt tagaagaatc acaagcattc ttgtcaaaaa
     7201 attatacgga tgatcctgag aattggggat tgatggaaga aaaagtttgg cgcaattata
     7261 cagcttttat gcaggaaaac ggattgatca agcaagatgt accttcgcaa gaactattca
     7321 ctaatcagtt tataaaatag gagtgataga tatgtcaatc caaatcagcc acttgaatgt
     7381 agtcattgaa aacctgaaaa tattagaaga catccatttc actatcccta aagatacctt
     7441 tacatcgatt gtcgcgccaa gcggagccgg taagtcaaca ttgctgaaaa ctttaacagg
     7501 agtccagcct atgacttctg gaacgatcaa agtagacgat caaaaagtaa ctggtctcca
     7561 tacagcattt agttatatgc cgcaagaaga tatgttactt ccttggctga gcgtctatca
     7621 aaacgttacg ttatatcaaa aaatcaatca tctaacaatc aatgaagaac aagtaaggga
     7681 ttatctggat atctttgggt taaacggcta tgaacatttt ttgccggaac aattatctgg
     7741 cgggatgaag cagcgaacgg ccttgttacg aacaatcatg aatccttctt cttacttgct
     7801 tcttgatgaa ccatttggag cgttagatgc tatgaccaga gggcagatgc aagactggat
     7861 gttgaagctg cctaaaaaag caaagcggac tactttactt gtgacgcatg atatcgaaga
     7921 agcaatttat ttatcagaca ggattcttgt gctttccgcc cgtccggcac atgtgattgc
     7981 tgagattcaa gtgcctgaaa aacgacggtc acgtgaatgg ctactgcagc aatcagagtt
     8041 gaagcaaacg atttatcagt tattggcagg tgaattagat gttaagtgat gctcattgtc
     8101 atttagatgg gagtcagagt ttagcactcc ttcagcagga acacacgatt ttgacgatca
     8161 tcaattgtga ttcgccagaa gaatggaagg aaaacagaca gcttgcagca agtaagactc
     8221 aagccttaag ttacggtatc catccttgga aagcagatag ctatacgttt gaacaagttg
     8281 agcctttttt gaaaaaagca aggatcatcg gtgaaatagg gttagataat atttggacga
     8341 atgtaccgat gactacacaa aaaaaagtat ttgagcgaca gcttgctttt gcagccataa
     8401 atgagaaacc tgttgtactg cacacaaaag gatgcgaaaa agagatctta aattatatcc
     8461 aagcttaccc taatcactat ttgatccatt ggtattcttc tttggactat caacaagact
     8521 atattgattt aggctgttat ttcagcattg gtcttgattt gaaaaagaat ccagcagtct
     8581 ggcaactagc taaagcagtc ccaatcgacc acttacttat tgaaacggat ggactacatg
     8641 cagcagagtg ggctttaggg gggatttttt cagaaaaaga ttatgtctca ctgcttgaag
     8701 aacagttaaa gacaatcgct gagatcaaag agctatcagt tgaagaagtt aaaaaaaata
     8761 cttatcaaaa cttggaacgt ttcattagat taggtaaata aaaaagagat gtgacacaac
     8821 tagtgtcgca cctctttttg gctttctttg tataaaagta aactgtttga ttaat
//
LOCUS       DANKHL010000117         8819 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000117 DANKHL010000000
VERSION     DANKHL010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.121"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            47..1387
                     /locus_tag="PW101_001926"
     CDS             47..1387
                     /locus_tag="PW101_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2514321.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1605..2048
                     /locus_tag="PW101_001927"
     CDS             1605..2048
                     /locus_tag="PW101_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDL2514322.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2552..3970)
                     /gene="nhaC"
                     /locus_tag="PW101_001928"
     CDS             complement(2552..3970)
                     /gene="nhaC"
                     /locus_tag="PW101_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL2514323.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4373..5596)
                     /locus_tag="PW101_001929"
     CDS             complement(4373..5596)
                     /locus_tag="PW101_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDL2514324.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5627..6466)
                     /locus_tag="PW101_001930"
     CDS             complement(5627..6466)
                     /locus_tag="PW101_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL2514325.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6483..7163)
                     /locus_tag="PW101_001931"
     CDS             complement(6483..7163)
                     /locus_tag="PW101_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2514326.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7165..8238)
                     /locus_tag="PW101_001932"
     CDS             complement(7165..8238)
                     /locus_tag="PW101_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2514327.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8294..8532)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8575..8808)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         2522 a         1809 c         1606 g         2882 t
ORIGIN      
        1 tcttaatata aattttgatt gtgaatttaa aagaaagagg tcatttatgg aagataaaaa
       61 attgaaaaaa caacttagct ccagacatat caccatgtta gctttaggtg gagcaatcgg
      121 tgcaggtcta tttaaaggaa gtggcgaagc gattgggatc gccggccctt ctgtattgat
      181 tgcttttttg attggtgggc tgattttata tatcgtgatg aaaggtctgg gaaaaatcgt
      241 tttaagcggt ggcgacacgc atcacggatt atctggtttg atacgccctt atctaggtgc
      301 tcattctgct gattttactg attgggtcta ctggtctatg tggattatca atatcatcgc
      361 tgaagctgtt gcagctgctt cctttttgca gttgtggttc ccgcacattc cagcctgggt
      421 atttgttttt atgttggctg ttttaactac gatcatcaat ctttattcgg tacggttatt
      481 cgcagaaaca gaatattggc tggcttttgc taaaatcagt gtcattatct tactgatcat
      541 ttttgctact tatcttgtcg gtcagcaaat gttgggcaca ggcgttttcc ctacccttca
      601 gcaattgact gaccatggtg ggtttactcc tcatggtatg aagggaatca tcagttcttt
      661 gcttgtagtt atctattctt acggcggttc tgaactgatt gctatcacgg taagcgaagc
      721 agatgatcct aaacgtgcga ttcctaaagc aatcaaaggt gtcatgggaa gaatcatttc
      781 cttttacatc attcctttgt ttttgctttt gattcttttt ccttggaata ctttagcagg
      841 cacgaatgtc agtccttttg tgatggtatt tgaaaaaatg aatatcccat ttgcagcgga
      901 tatcgtcaat tttgtgattg tccttgcttt gttctcatct atcaattcgg gtgtgtatgc
      961 atcttcaaga attttgttct tccgtttgaa ggatcgaaaa ggttcctcta gaaaactagc
     1021 tgttttaaac aagcatcaag taccgcaacg tgctgtttta ttttgtacca gtgttcttta
     1081 tcttggtgta gctctttctt attttgtcgg tgacaaatta tttggctatc tggcaggttc
     1141 cctttcgtat acagtattat tgatctggat cattatcagt gcggcatcct ttgtactggc
     1201 actaagaaaa ggaacattat gggataaagg aatcagcttt tttgcattag ctgtcttagg
     1261 attgattttc cttggtatcc tgttcacgaa ctccattggg gtaactgtat tgactggcct
     1321 gctttacctg ttcatttatt tcagttatca aaaaaagaat gatgcgttcg tattgacgaa
     1381 tgaatgaaat cgatagaaaa ttttatttta tttatgtgga atagatggag ctattgaaga
     1441 aaggagttgt gacaaaagtc ttgttgcaac tcctttttcc gaataaacag tctcatgacc
     1501 tcttggtaaa cggtgttcaa aaaccctctg tgcaaaataa ttgattgttc tccatgctca
     1561 tgacatgata gaagaaagga atgaaaaaaa ggagtttgtt ttaaatgaaa aagtattgga
     1621 agtttttgtt gatgatcggt gtttcaacgg tagttatgtt tggtatgatg tacttgaatg
     1681 tctatgcgct cgatcatatc ttcttcagtc gtacacgagt attcatggcg ttgatgatgg
     1741 gtgcagtcat ggcagtcatc atgctgttgt tcatgtggaa aatgtatgaa aacaaaaaga
     1801 taaatgttat gatcttagcc gctagtcttc ttgtttttgc cggttccttg tttatggtac
     1861 gaagccaaac tactgtcaac gacacagctt ggatgaaggc catgattcct catcattcta
     1921 tcgctatcct ggcaagtaaa cgagctgatt taaaagatcc tgaagtaaaa aaattagcgg
     1981 aagaaatcat tcaggcacaa gaagaagaga tcgacaaaat gaaaaaaatg atcaaagaaa
     2041 tggaatgacc attatccgtc caaataaaaa aagaggatgt tccagaagtt atcagcttca
     2101 agaaataaga agaagctact tctggaacgc cgtgaataag gggttgtgaa aaagctgccc
     2161 tgcatcaagt aataagaaca gccaaacaaa aatagcttct cctatttttc gtttggttag
     2221 gcttattacc gcagatgcaa gcttttgaac accgtttatt aagaggttgt aaaatcagcg
     2281 ttctgacctg agtattagag agacaaatgg aaaaatagtt cctcatattt tgccattttt
     2341 tgagctaata ccgagggtca gctgattgaa caccgtttat cgagaggttg tgaaaaagtc
     2401 gcttagtttc gcgtattaag aaaaattttg tgaaatggct ttccacattt ttgtataaat
     2461 ttggcttaat accgagaaac tggcttttga ataccgttta ttcgtaaaag ggattgtgac
     2521 aaaagtcttg tcacaatccc tttttatgtt tttattgttc taattgcgct gtcttgctta
     2581 ctgcttcttg cggtttcaca aatttttttg agattcccca acctgtgaag gcactgacca
     2641 tcgaaaacac aggagaaagc aaactgaaaa atacgaatgg caagtaagtg agtgtaggaa
     2701 taccaaatgt acttgctacg aaacttccgg caattcccca aggcactaag tagttgatta
     2761 ctgttccacc atcttctagc gttctaccta atacagttgg atcaaatcct gctttttcat
     2821 aagtttcttt aaatgcatta ccaggcagaa taacagatag gaattgttca ccaataaaaa
     2881 tatttactcc gatacttgtc atcaatgttg taaaaatcaa atttggtgtg ttcgtcatct
     2941 ttcttgatac tcgttccatc acggttgaga tcaagccaaa ttcgatcaac aatccaccta
     3001 atgacaaagt tagaataatc aatgacacag taggcatcat actttcgatt cctccgcggc
     3061 taagcagtga atcgatttgt tggtttccag tatttgaaac aaaccctgat tcgatgaccc
     3121 cagccaactt cgttaaggaa gtttgtgggt tttgaataaa aatcattcct actgctacag
     3181 cgatattcaa caaaatcgtt aaaattgccg gcaccttttt ccatgcacac agtaacatta
     3241 aaaataaagg aatcagtgac cagattgaaa tagtaaagtt tgcttctaga acatcagtca
     3301 cttcacgaat agatgaaaga cttgcggctt tacttgtgtg tcctaaaatc atgaataaaa
     3361 agaaagaaac aacaaatgcc gggacagttg accacatcat atgcttgatg tgtttgaaca
     3421 aatcggcatc aacaatagct gctgctagat ttgttgattc ggataatgga gacatcttat
     3481 ctccaaaaac ggcacctgaa acaatagctc caacaaccag cgctggattt aagcctagtg
     3541 ttgtcccaat accgaaaaat gcaatcccaa tcgttgacat aacagtaaag gcactcccta
     3601 cagcgctacc tacgattgca cacacaacaa atacagaagg gacaaaccac tggacactga
     3661 tcaatttgaa cccaatgacc attagagttg gaatgatccc tgcttgtatc catacagcaa
     3721 tcaatgaacc gactaaaata aaaataaaga ttgggatgat tcctggtttg atgccattga
     3781 tgattccttc gtgcagccaa tctgtcgggt atctttttat cactgcatac aacatgacca
     3841 ttccgatcgc tgctaagatc gtgatattag gtgagacctt taatccgaca acgccaatcg
     3901 caatgcctaa aacaataacc attaatacaa gtgttgcttc taaaaaactt ggtgttcctt
     3961 tttttcccat gataatctct ccttttttag ttgttcttat gttctaaaaa agttaagata
     4021 tcggattact ccgccgcaat tcatccgcga ttcctccttt ataaaaataa aaagacctgt
     4081 tttaaactaa ggacgaatga ctccgcggta ccaccttaat taaaaacaga tctgtttttc
     4141 actttcaaac ttttattcaa atgtatggac tttccatgta attcgtcaat taggcttgat
     4201 cggttcacag cgaccaccga ctttctgaac aaaacaccta accgctactg cgtgaaaagt
     4261 tgacttgtac gaagtttacc ttccataaga tcatttgtca atgaaaaaga aaatattttt
     4321 tcatttttct gaaaattatt tttataactt tcacacacaa gattattttt tactagaaag
     4381 tagcgccctc ttgttttttt cttgctacta tttcttgatg ttcttgcttg attctttcta
     4441 atagctttgg ctctttcaac agatcaagag ctgtattggc gagtagttct gctgcaatcg
     4501 caatagaagc aaggcctttg tcacttttag cagcagcttt gaattcttct gtgtgtccgg
     4561 caatatatgt ctcagagatt gctaccgtag gctggattgt tggtatgact tggctgacat
     4621 ttcctacatc agaagaaccg agacttgttc tttgttccgt ttctatttct tcatctggaa
     4681 cacctgcttc ttttgcatgc tggaagaata agtcatcaaa agaaggagta acgatgacat
     4741 catcaactgc attttggaac aatccaaact caaaagtcgt gcccgtactt atcgcagcac
     4801 ctcgtacgat attttccact ttttcatata ctttatctaa tgtttgccga tttcctgcac
     4861 gtaaatagaa acgtccagca gcgtaatctg gaatgacgtt ggcagctact cctccgtctg
     4921 taatgatccc atggatattg acatcttttg gcaattgcaa gcgtagagcg ttgattccat
     4981 taaacacttg gatcaatgct tccaaagcat ttactccttt ttcaggtgct gctgctgcat
     5041 gggaagcgac accgtaaaat ttgatgtcga ctggatcatt cgctaaagat tctgtcgttt
     5101 tcccataacg aaaagctgga tggacgcata acgctacatc gacatcctca aaaaaacctt
     5161 ctcgaacaaa gctgccttta gctgatccgt tctcgccccc ttcttcccct ggggtgccat
     5221 atacacggat ttctccacca gtttcatcaa tgaattgctt cactatgctg gcggctaata
     5281 cggagtaagt accaaacaaa ttatgcccgc atgcatgacc aatacctggt aaagcatcga
     5341 attctgccaa aaaggcaatt gtgggaccgg gtttattgga aatataacgt gcatcaaagg
     5401 ctgttcggtg tccagctact tctttttcta cccaaaacct ttctttttct aattgtttaa
     5461 tcaacgctgc tgaggaataa aactcatagt tacttacttc aggatgttcg tggatatcta
     5521 gagcaagtgc ttgatattct ggtaaacgat attggattag atccttcaat tggtttgttg
     5581 agattgttgt tgtcataaaa aattcctcct atgctagtca tttttcttat tttctcccga
     5641 atgtttccca tgcaggcacg cttgatcctt ttgatgtttt ctcgattact ttgactgtgt
     5701 cttctgtttg ataagcgtct actatttttt gatacgtttt attgttttca tcttcttttc
     5761 ttgcaacgat gatattcaca taaggacgtg catggtcatc cactggttct aagaagatgg
     5821 catcagaagc aggattaaat ccagcatcta cagctacacc gctattgatc actgaaatgt
     5881 ctacatctga caaagcacgt gctgtttggg aagcatctaa ttcagaaatg tctagatttt
     5941 ttctattctc ggtaatatca gaaacagtgg gcgcttgtcc ttttgcctta tccactttga
     6001 tcaatccagc agtttgcaat aacagaagag ctctgccgcc atttgtaaca tcattaggga
     6061 ttgctacttt tgctccgtct ttgatatcag ctacatcctt caaagtgtta gaataaattc
     6121 ccagcggtgc attgacagta tttccgatcg atactaaatc agttccgtgt tctttattgt
     6181 aattgtctaa aaagaattga tgttggaaag aattcaaatc aatcgatcca tctgctaaag
     6241 ccacatttgg ttgattgtaa tctgtaaatt tcacgtattc caaattaatg ttctcctttt
     6301 tcaaacggtc tttcacgttg tcccatacat ctgtatcttc tccaataatc cctagtttta
     6361 cgatagtttc ttttttgtca gttgtatctg cttttccttg gctacaccct gccaaccaaa
     6421 taattgctac aaatgtaatg actacaaaac gaataatttt tttcattttt tctcctccac
     6481 tttcaatgtg taattttttt aatcagttga ttactgataa attgacttaa aaaaactaag
     6541 attagaatca ataatgtagc cacaatcgtt acatctgttt ggaatcgatt ataccctctc
     6601 gatatagcaa gattccctaa tccgcctgca cctactgctc cagccatggt agtcagtccg
     6661 atcacgttga tgatagtcaa cgccgagacc cgaatgatac caggcaaacc ttctaacaaa
     6721 tacacacgta aaatgatacc gattggactg gttcccatcg cttctgccgc ttcgatcacc
     6781 ccaggatcaa cttctagcag tgcgttctcg atttgacgag caaaaaacgg aacaactcca
     6841 aaaatcaatg gcactaaagc tgctttcgca ccaatcgtcg ttccaactaa gaaacgagta
     6901 gtcaatgcta aaagagaaag caagataatg aaagggatgg aacgaatgat attgatcagc
     6961 ttatcaagta tttcaaatag cagacggtta gctagtaatc cattagggcg tgtcactact
     7021 aacgtcacac ctaagatgat tccaatcatc ccgccaatga atgctgtcca aaaagacata
     7081 tatagtgttt gcttcgtcgc ttcgacaaat tcatccggaa tcaaataaac attgggcaaa
     7141 tatgttttaa agaaatcagt cattttatct tcctccttcg atcactttga tacgactatc
     7201 taaatcgttt ttttgtaaaa tatttatttt tacaccagat ttgatcaaat aatccaatgc
     7261 ttttttccgc tgtttggttt ctccagctaa agtaacgatc aaactcccta acggcgtttc
     7321 ttgtaataac tctacattgc catataaaat attcgctgtt acttgatatt tgctgtacag
     7381 atgagcgatt agaggttcgt tcgtctgatt tccaatataa cttaattcca ccagccattt
     7441 attggcgatc tgatcggcaa aagcagaatg ggcaatgatc gtttctaatg cttggtcaag
     7501 atgagtagct gttcgaataa aatctttggt caattcttct tcaggattgc tgaagatctg
     7561 gatgctgcta cctttttcaa cgattcttcc atcttccata accgctacct tgttgcagat
     7621 ttctttgaca acttgcatct catgagtgat caggacgatt gttaaaccta actggcgatt
     7681 caattttttt aacaatgcaa ggatctgaag ggtcgttttc ggatcaagtg cactggttgc
     7741 ttcatcacat aataatacct taggttcatt cgccaaagct cgtgcgattg ctactcgttg
     7801 tttttgtccc cccgacagtt gtttaggata agcagatgct ttttcttcca aaccgacaag
     7861 ttccagtagt tcattcactt tttgacgacg ttcctgtttc gattttccag aatactttaa
     7921 agaaaaatca acgttgtcaa aaatagttcg gctttccatt agattgaaat gctgaaagat
     7981 cataccgatg gcctttcttt cttttcgcaa ttctttggga gagagctgac tcagattcgt
     8041 ctgattgaca atgacttctc ctgctgttgg tttctgcagc aaattcatca cacgaactaa
     8101 cgtacttttc cctgcacctg agtaaccgac gattccataa acatcgcctt tttcaatcgt
     8161 taaatctaca gaatctactg cctgtatctc tttttctttt tgtttgaaac atacagaaac
     8221 atctcgcaat tcaatcatta aaattcctcc ttatctaaaa aaagcgtcct caagaaaatg
     8281 aaaatgtcat tttcttgagg acgctttttt gcgtgttacc acctcatgtt caagctgtgt
     8341 tcacacacaa ctcctcttca agtacgatca tactctagca agatatcggt cgcagccgtc
     8401 atcgccttac atctatgctc gggatgaatt gctcgatggc cattttccaa tagtcgttcc
     8461 ttgcttcttc tcagctaccg aagctctctg tcaaaggttc ttctatcgta cttaccattt
     8521 caatgcaaat aatattcaaa aaaaaagtcc ttttactaac ataaactatg tcagtaaaag
     8581 gacgacgtat cgcggtacca ccttttttcg gaagtttcct tccctcaagc ccaacatctc
     8641 aaaaagagag attggcgttc gttttaacgg tcgaacagtt ccgaatttgc ctacttatcg
     8701 acaaattatt ctccaaggcc attttcagct tttcttactt gactcttctc accagccaga
     8761 gcctctctgc aaagtttcca aacttacttt ccttttcaac gaaatcgata ttttgttgt
//
LOCUS       DANKHL010000118         8791 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000118 DANKHL010000000
VERSION     DANKHL010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.122"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            6..305
                     /locus_tag="PW101_001933"
     CDS             6..305
                     /locus_tag="PW101_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514328.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(402..836)
                     /locus_tag="PW101_001934"
     CDS             complement(402..836)
                     /locus_tag="PW101_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2514329.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1068..1778)
                     /locus_tag="PW101_001935"
     CDS             complement(1068..1778)
                     /locus_tag="PW101_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL2514330.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1768..2628)
                     /locus_tag="PW101_001936"
     CDS             complement(1768..2628)
                     /locus_tag="PW101_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDL2514331.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2633..3271)
                     /locus_tag="PW101_001937"
     CDS             complement(2633..3271)
                     /locus_tag="PW101_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDL2514332.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3286..3585)
                     /locus_tag="PW101_001938"
     CDS             complement(3286..3585)
                     /locus_tag="PW101_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2514333.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3628..5112)
                     /locus_tag="PW101_001939"
     CDS             complement(3628..5112)
                     /locus_tag="PW101_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL2514334.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5132..5593)
                     /locus_tag="PW101_001940"
     CDS             complement(5132..5593)
                     /locus_tag="PW101_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2514335.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5611..6678)
                     /gene="ulaG"
                     /locus_tag="PW101_001941"
     CDS             complement(5611..6678)
                     /gene="ulaG"
                     /locus_tag="PW101_001941"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDL2514336.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6896..7666
                     /locus_tag="PW101_001942"
     CDS             6896..7666
                     /locus_tag="PW101_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2514337.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7939..8766)
                     /locus_tag="PW101_001943"
     CDS             complement(7939..8766)
                     /locus_tag="PW101_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2514338.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2661 a         1853 c         1383 g         2894 t
ORIGIN      
        1 aaaacatgga ttatactgtt gaatttgaca gtgaactact taagcaattt ccacaagaag
       61 aacatttcat gtataaagaa gcaatttccc acaaagtgac agagtatttg gagcataatt
      121 tttttcgtat aaagccagtg cgtactcgtt cggcaaaagg aatatacgag atgaaagtca
      181 aaattaataa aaaaaattat cgcatagcat ttgctttaaa aaaaaatcat gtgttcattt
      241 tttatattag ttcgaatttg caaaaaattg tatttgataa agaggtgagt aaaaaaataa
      301 aatagctgag cattggagga tagattgagt gatactaagt ctatcctctt gtttttctaa
      361 agaactaagc tagaattttt tcttttgtac gttattatat gttatttgtc attaccaaaa
      421 agcacctttt ttcttctagt atctatgaca taagaaatac cacccatttc ctttacttga
      481 acaaatccta ctgattcata aaattttttt gttttctcag tatcatctgt caataattcg
      541 atttggactg gtccataact agtgatcttc tccaaagctt cattcaaaag cactttgcct
      601 attccttggc gctgatacct cggatgaatg aggatatctt gaataaatgc gatcgtcgca
      661 aaatcagtga tccaacgaac gattccgata atcttccctt ccccattacg ctttatcaga
      721 gattcagatt gttcaaatgc tctagctaac cttgcagtgt cttttgtgta attcgtccaa
      781 ccaacgctct tatataattc agaaatctca tcgatcataa gggaatcaat attcattttt
      841 ttcttccttt cttatacaat tctccctcta ataatacagt tctttgacta tttaatctat
      901 cacatataac tctcctgtaa agattctctc gattccttaa aattcctctt tccttttaga
      961 atgcatactt tttgataaat aaaaaggttg tgataaattt tcttcacaac ctctgtttct
     1021 ctctatcaag tgttatcttt taattttcta cccttaccct agcatctcta ttgcccgtag
     1081 tatgcatact ttccatgttt gcgaaaataa tgtttatcta gtaaatattg cgggatttca
     1141 catatattgg ggttgatcag ctcgttttct ttggccatca gacaaatttc atctaagatc
     1201 aaactgtttt ctactgcttt cataggtgaa tccccccatg tgaacggtcc atgtccatag
     1261 acaagtactc cagggacttc gtttggatct aattttcttt ccttaaacgt ctcgacaatc
     1321 acattccctg tatgaacttc gtaagcttct cttacttctt ctttcgttaa ttggcgtgta
     1381 caaggaacct taccataaaa agcatcagca tgagtcgtgc cataagctgg caaatcgcgt
     1441 cctgcttgcg cccacatgac ggagtgcgtg gaatgagtat gagtaatagc agttatatct
     1501 tcaaaagttt gatacaaaac aacatgtgtg gggagatctg aagatggttt taagctatct
     1561 tcttctatta tatttcccga caaatctgtt accaccattt gatctgcttg catgcattca
     1621 tatctgacac cgcttggctt aatgacgatg actcctagct cacggtccac ttcactcaca
     1681 ttgccccaag tcaatttgac taatccgtat cgcggcaatg ccagatttgc ttcaaggaca
     1741 cgttgcttca ttgctgcaat tttttcttca caagacatca tagccagcct ccttcaattt
     1801 agaacttaag tagtcttttg cctgctggat ttctcttcga aagtcttggc ttttttcact
     1861 ccacatttca atcagaaatg ttccatcata atctagacgt ttcaaatgtc ttaataatcc
     1921 aagaaaatca acacaaccgc taccaaaagg aacatcacga aactggcctg aatattcttt
     1981 tgttacaggg taagtatctt ttaagtggat agcagtgata taatcgatcc ctttctcaag
     2041 ttctcgagcc ggattatttt ccggccatgc ggacaaattg cctaggtctg gatatacttg
     2101 tagaaaaggt gaatgaatct gttctttgat ttctaaaaat tttgaaatgg agttcataaa
     2161 tggatcatcc atgatttcca cggaaagaat gatgccatat ttagaagctt cctttacgct
     2221 ttcttttatt ccttcgataa agtcttctct ggaattcatt gatttctctt cataataaac
     2281 atcgtatcca gctatttgga ttactttgac gcttagatta tgcgccaaat ggattgcttt
     2341 tttcattagt tgtttcgctg cttttttctt ttctggatca gcagaaccaa acggaaatcg
     2401 ccgatgcgca cttaaacaaa tcgaatggat acgtacaccg gagctgaaca tctcttctct
     2461 cagctcagct atcttttctt cagaccaatc taatcgtgct aaccgctcat ccgtctcatc
     2521 aatcgacatc tcgataaaat cgaagtccat ttcttttaca agggaaaaac gttctttcca
     2581 tgaaatatct ttgggtaacg ctttttcata aatcccaatt tgagccattt ccttaccccc
     2641 agatacgaat gatctcatct ttgaagtttt gtgcggcctt catcggatca tttgctgctg
     2701 taataccacg accagtgata aaggtataca catcgatccc ttcaaataag tgtaatgtgt
     2761 gtggatttaa tccaccagtt acagaaacat taaagcctag ttcaatcagt tttttgatct
     2821 tcgataaatc tttttcaccc caagtttcac cagataacag cgcatcacgg ctttgatggt
     2881 agattacctg ccggataccg atgttatgcc aatccatggc ttgttcaaaa gtccaattgc
     2941 cgtagagttc tacttgcaat tcacctattt ctttttgtgc tgcctccata gttggaatcg
     3001 ttgcacaaca aatgactgtc atccagtctg ctcctgcttt tgcaacatta ctcgccactg
     3061 ttcctccagc gtctgcacat ttcgtatcag cgactagttt tttatctggg aacatactgc
     3121 gaatacagcg aatcgctttt tgtccttcct gtaagcaaag aattgtgccc acttcaataa
     3181 tatctactac atcaccaact ttcataacat ctcctaatgc atgttctaag tcattgtgat
     3241 ctaacgcaat ttgcaaattc ggcctgctca tcttagttcc ttcctttact tgtttttttg
     3301 gtaccacttt gtttcagcaa atttttcact gatttctttg actgacatca catttttgat
     3361 accgataact gtaactcctt ttttctctgc ctgagtaaac atattcaaaa aattgactgg
     3421 cgtaaaaact acatcgtatt ggccagctga actttttccc tctgaaatgg cacaatgatg
     3481 gattttggta attggtacgt ttagttcttt cattgctttt tccacacttc tcatcatcat
     3541 caagcttgtt cctgatccat ttgcacacga tactaaaatt ctcatcttaa tccctccatt
     3601 atgagtgttg tctaatcaga ttttctttta tactctctgc ttttctgcat atgtgtcata
     3661 gtcttctaca atcaaaaaat aaccatcagg atttttccga tattgtaact gcggaatagc
     3721 caataaacca atgacaacta cggcaattcc agcataacct aggactttca tgattactgt
     3781 aaacaatggc caagctgttg cccagtcgaa catcccgata taaccaccgt atgttgacat
     3841 cccaatccat gtagcgaata atgctgaacc gcctacttgt atgattcctg aaagaaacgg
     3901 gaacaggcaa gcggctttga atccacctcg gttattggca taaactgcta taacggcatt
     3961 atcaaagaac aacggaatga aaccggctat gacgatcgtt ggtgatttca ataaaatcag
     4021 taaaccaatc atcaaaaact gtcccaaagc accaaacaag aacccaatcg ttaccgcatt
     4081 tggcgaacca aagccaaaag tagcagctac gtcaattcct ggaacagctc ctggtaaaat
     4141 cgtgtttgag attccttgga aagattgagt tagttcatcg acgaatgtcc gaacacctaa
     4201 ttgcaagatc gccagatata ccgcaaaatt taaagaagtc gtcataatgt agaataagaa
     4261 actctgccct tcttgcatga attgcgcttg aatcaaataa tctttgccta gtacaatcag
     4321 aatgatccca aagaaaaata gcatgagtaa cgaagtagca accatattct cattgaaaat
     4381 cgacaagaat cctggtaatt tgatatcttc aatttttttg tcttgcttga ttgatctatt
     4441 tttcttttca tcccgctttt tcatccactc agctaatttt gcaaatataa agattccgaa
     4501 catttgctga tgtgccactg caaatccggc accttctgtc aattcctgcg tgattcctac
     4561 tgttaagtta gaagctactg tccaataaca acctaaaatc agtcccataa aaatcaatgc
     4621 ttgtatccta cctagatcag gaaagcaaaa cagtaaaatc caaaaagcgg tagctgcttg
     4681 ttgaacctga acgtttccgg tagtgaaaac ggaacgcagt ttcgtatatt tctgaaaacg
     4741 gactaataag atatttaaaa caaatgcaat cagtaataaa atcatcacat ctccaaaggt
     4801 acggccaaat gtctccatta atccagcatc cacagcattt tgtccaaaat aaggatctgt
     4861 aaccatggca tccaaattga accgttcctt tagtccaacc aagattggcc gaaaattatt
     4921 gaccaaacca ccagagccaa cagtcaaaat aaagtaaccg accgttgcct ttaagaatcc
     4981 agcgatgcat tcatgaagcg ggcgtttcag caaaatatag cctaataaca cgataaaacc
     5041 aatcaggtat gctggctgcg tcaagatatt tgttgcaaaa tacgtccaaa tgcttaacaa
     5101 aaattccccc atcttttttc tcctctatca attaattata ttctgccatc acaacttggt
     5161 aatcatcgat ggagttcgta gagatgagac gctcaaccaa tccatctgtc attaataatt
     5221 cggataattt ttggatattt tctagatgtt cttctggatc ttttgctgcg agtgtaaaaa
     5281 acaaactagc cgttttctcc tcattctccc cttcactttc aaaacggatt ggctgtttca
     5341 acttggtaaa tgaaatagct gttccaaaaa caccatcact tttttctgaa gaatgcggca
     5401 ttgctacttg aggtgcgatc acaatataag gaccgtattt ttctacacaa tggatgatct
     5461 cctccacata cgtttcatca ataatttcct tatcgatcaa tacaagacag ctttcggcga
     5521 tagcttcacg ccaatccgtg atttctttat ctgggtaatg aatcaattga tggtcaaaaa
     5581 agtatttcag caagaaactc cccttctttc ttataaaaac gacggaaacg gtgtatcatt
     5641 ttcaatcgag aagacatctt caaatcctcg ataaaaatta aactcaagat catctttgtt
     5701 tgtcggatag atgaatttcc cgcctacttg ccagatataa ggtttaaatc catacttcag
     5761 gcgatccttt ttgaatttcc aaatttcagt gatctctttt ggatcagcca taaaattaga
     5821 ccaaatatcg tagtggacag gaatcactac ttttgcattc aatgactccg ccatgcgcag
     5881 catatcaaca gaagtaactt tgtctgttat ccctcgtggg ttttcaccat aggcccctaa
     5941 acagacatcg attttgtgtt cattaccatg tttggcaaat aaattggagt aatgtgaatc
     6001 tcctgcatga taaagattcc ctcctgatgt ttcgaataaa tagtttactg caatttgatc
     6061 catctcctga ggcatttttc cttttaatac ctcttctgga tcttcacaag tgaccaacgc
     6121 tgtccgatca aacgcttcta aagctactac attgatgtct ttgattttga ctgcatcacc
     6181 tggctttact gtaatcgttt tttcttcagg aattccccat ttttgccacg tgcgaaccac
     6241 ttcttttggc ccaatgaatg ttgcttcagg acaattttga tgaactgctg cggctgtatg
     6301 gatatctaga tgatcggaat ggatatgtgt aacgattaaa gcgtctacat tttttatcgc
     6361 aaaaggatcg attacgaaag gctgagtacg taaattaggc tgcatatttt gaacgccact
     6421 catacgcatc atctgatggc cttttttcat catgccattc ccatgactac gttttcccgt
     6481 cccacaccat agatcacata aaatattcgt tccttcatga cttttcagcc aaattcccgt
     6541 acagcccagc caccacatag caaaagttcc aggatccact acttctgttt cgatttcttc
     6601 attcaagtat gtgccccact caggaaatgt ttctactaac cacttttctt ttgttacttc
     6661 attgacagtt ttactcattt gcttgctccc cccatatcgt tgttccaact tacaagtaaa
     6721 tattaatgtg tatcagcatc catgaaaacc cttttatatg accgtttgtt ttttatatga
     6781 ttttctttca gtaatcaatg attgtttcag tataataaag acagatatga atgtttaaat
     6841 gaacaactag tcaaataaat ccatggatca agatgtccct caggaggcaa aagtcatgaa
     6901 aaactcactt gtcaatattg aaaaacgtca aaaagaaatt ttggaactgc ttcagcagac
     6961 tgaacaacta tcaactttcg aattagctga atcccttgat gtatctatta gtacgatacg
     7021 aagagattta aatttattag aagaaaaaaa cgagatcata cgcaattacg gttattgttc
     7081 ttataattac aaaaaccaga ctgattttga tcaatctggt cctgaaagga ttaaacaagc
     7141 gattgccaga gaagctagta aatacattaa cgattatgat acgctgttta tcaattctag
     7201 ctcgactgcg ttgaaaacac tggattttct tcaatctaac catttgacag tcattacaaa
     7261 caacgtgaaa atcaattact ctccgcacaa agataattgt agttatattt taactggtgg
     7321 agagatgcgc tttccaaaag aagcactagt gggcgacatc gcattaaata cgatttcttc
     7381 agtcaatgca gacatttgta tcattggctg tagcggagtc gatgtagtac atggggtcac
     7441 aacaaagata ttaaatgaat caacgatcaa cgaaatgatg gtcaacaaga caatcaaatg
     7501 taagatttta gtcgctgatc atcgaaaaat cggactgaca tcaaagttca agattgctga
     7561 tatttcagtc tttgattatt tgatcactga taaattttgt tctgaaaaag tattagagga
     7621 gatcagaaat acggggataa aaaccattca agtagattca ttttaagaac caggacgtaa
     7681 atatgtaaat aggattatca aaaaaatgaa taacaaattt atcgataaat gaaaaacacg
     7741 ctacgctcaa attgataaag gaatcccaag atgtatccat ccaatcgata ccatcataat
     7801 ctattgcttc ttttataacc gttatatgtt gcctaaatag gaatcaaaca atatcgaaaa
     7861 aaggaggtat gacaaaagtc acacctcctt ttttcgaata aacggtattc aaatcagaac
     7921 actgatttca caaccacttt atttcaggtc gttttcaatc acctttaccg cgtaatcatt
     7981 tccgttgaaa cgcgctattt tttttgcgtc catatatctt tctaaataat catcttctcc
     8041 taattgctta tggcaagaag caatttcata aagcaagtat tccaatgcat aagtcgattt
     8101 atgtttctta ttatactcaa tacccttctc acataattgg attgctttat cataatcctc
     8161 gattttacgg taataggtgg cgatatcata atatacaatg cagaccatca gattatcaaa
     8221 atcagtctca ctcaatattt taagtgtacg ctccacataa actttcgcaa aatctaattc
     8281 tccttttgac aagtagatca ttgccacgtt tgcagctgct tgtaagtgat ggatatctgt
     8341 ttcggtcgtt tgttccaaca cttgattaaa ataaaataat gctgcatctg gttttttcgc
     8401 ataaagatga tcaagcagtc cttgataaaa taaaatttga tttaaaagat cagaattact
     8461 tactttttcc tgatcgatcg tttgtaaaat tttataagat tcttggtatt ttgatacaga
     8521 attcaatgat tgaatctcat ttattttttt cctgattttg tcttcttctg tttctactaa
     8581 tatatcggag atctcgacat tcagcttttt gcaaagcgca cttaagatat tgatgcctgg
     8641 cgacatattc ttgttttcga tattgctgac agttgcttgt tcacaaatgc cttctgctaa
     8701 ttgtacttgt gttaacccct ttttctttcg gtattctttt agtaatgctc catctacttt
     8761 aatcaaaaca atttccttcc ctttaaaaaa t
//
LOCUS       DANKHL010000119         8766 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000119 DANKHL010000000
VERSION     DANKHL010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.123"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(221..1177)
                     /locus_tag="PW101_001944"
     CDS             complement(221..1177)
                     /locus_tag="PW101_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDL2514339.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1281..1883
                     /locus_tag="PW101_001945"
     CDS             1281..1883
                     /locus_tag="PW101_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2514340.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1971..3470)
                     /locus_tag="PW101_001946"
     CDS             complement(1971..3470)
                     /locus_tag="PW101_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDL2514341.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3486..4010)
                     /locus_tag="PW101_001947"
     CDS             complement(3486..4010)
                     /locus_tag="PW101_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDL2514342.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4308..5057
                     /locus_tag="PW101_001948"
     CDS             4308..5057
                     /locus_tag="PW101_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL2514343.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            5048..5833
                     /locus_tag="PW101_001949"
     CDS             5048..5833
                     /locus_tag="PW101_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HDL2514344.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5802..6518
                     /locus_tag="PW101_001950"
     CDS             5802..6518
                     /locus_tag="PW101_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2514345.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(6620..7654)
                     /locus_tag="PW101_001951"
     CDS             complement(6620..7654)
                     /locus_tag="PW101_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HDL2514346.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(7658..8716)
                     /locus_tag="PW101_001952"
     CDS             complement(7658..8716)
                     /locus_tag="PW101_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL2514347.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         2630 a         1781 c         1578 g         2777 t
ORIGIN      
        1 tggacttatt accgcagagg aaagcttttg aacaccgttt aagaaagagg ttgtgagcct
       61 accgttcagc tccgagtcac aaggagcaat ttcaaaaaac agcttctcat gtttgttgaa
      121 tattgtgact tgtgaccgag gagttgacag gagaacaccg tttaaagaag aggttgtgaa
      181 atcagctttt tgacctgaat attagctgaa ttcacaacct ctatttttgg attttttgtt
      241 gggagaattc tgccttgatt tcaataaaac ttttaaaaga ctgcaaaaac tggaacaatt
      301 ctgctcgatc ctcgaattca gggcgactag tcggcagagg catttgccga tacaatacat
      361 aaacttcttc aatacgtgtt aaaatttgtc tgccatcatt tgactctgaa aatgtttctg
      421 cagtataaat caataaacca tagatatgtt ttccataata agctggttgc tggattctct
      481 ctagattttc cagcatgtct ttaatcacat tggcctgtgc tcttctcata gagaagtaag
      541 tttcgtagta tagcggctgt ctgagccagt agttttcttg atgttctctg gcatcttctt
      601 ggctttcgcg aatgaatgtc agtaaatgtt cgcatttgat ctgcacatct tctttatttt
      661 ctgataaaag aaattctgcc atttgtctca aaatatttcg aaattctttt tcgatctgta
      721 tttggttttc cttcagctgc ttcgtacgat ctggcatgta aacatttgca agcaaagcaa
      781 atactaatcc aatcgccatt aatcccgctt cattgccgat cagctgccaa gacatgtttt
      841 cctccactaa ataatgagtg accaaaaccg aatttactac tatcccttcg gttaatttga
      901 agcgagaggc aagcgggatg aacaggaata aaaaaagact gaagccaaaa atagtatacc
      961 caacagcaaa aaaactgaac agtgctgttg ccgtagctaa tataaaggct gcgattcgat
     1021 tcatgcccgt acgcaatgta gagcgcttgg tatttcctac acttaataat gcaatgatcc
     1081 ctgcagctgg ccaatacaac agcgataagc tacccgctgt gaccatcgaa aagaagacac
     1141 tgatcactgt tttgactgtt cgcattccta tacgcattta cttccccctt ttttatttta
     1201 attatacttg aataatgaaa aactagctat cagacaaaag tttaggtatg atagtaaaaa
     1261 gaccaaaaag gagaatctct atgcaatttg gccgttttcg actaggtatg cgtacgatca
     1321 aaagtgcctt agctgttttt ttatgtatct tgttttttca tttgactcaa cgaggaatcc
     1381 cgatgattgc tgcattatct gctgtttttt ctttgcgtca ggacttgact acaaccgttt
     1441 cttttggaaa gtctaggatc atcggcaata cattaggtgg tacattagct atcatttatt
     1501 tttttgtcaa aagctatttt cacaatgatt ttttggtaga attgatcttg cttcctattc
     1561 tagttattat tgtgatcgtc gtgtcagatg gtattaacaa taattccggt atcatttccg
     1621 caatagctac gttgttgctg attgcgttga gcatccctca aggcgaatcc gctttgtatg
     1681 ctatccaacg tgtactagat acttttatag ggacattcat tgcaatcggt atcaatttct
     1741 ttcttcgtcc tcctgaaaca gaaaagaagg aagaactatc agaagatttg gtcgaactcc
     1801 aaaagaaaga acgattgcta agaacaaatt tacaagagat agaagctaag atccaaaaac
     1861 aaaagaaaaa tagcgaggac taattttctt ttgcaataaa ataaaaaaat ggagagaaga
     1921 actttaaaaa gtcttcctcc atttttgatt gaacggtgaa gtgtactact ttattcttcc
     1981 ttcatcgagt tttgttctgc ctcttttggc gcttcataca tatttacttg gctagtagtc
     2041 ccacgtgtcg acaaattctc tacgatttcc ttcaagtcaa gtcctgttgt cgcttttagt
     2101 gtttcttgag tagaagaaag caaatttgtg gcataatttg tcactcggtt cgctccactg
     2161 gtttctcctg aactgcttcc tgtatcgaca acagagattt tttcgatatt tcctagcggc
     2221 tgtgctgctt cgcgcatcaa ttgcggtagc atatcgataa ccatactcaa gacagctgct
     2281 tcgccatatt ccttgaaggc attggcgatc ttttgttttg cttcggcttc tgcttttcct
     2341 tttgctagtg ctgcttcggc ttctgcttgt ccagctaaac gtgtcttatt ggcttccgct
     2401 tctgccagtg cttccacacg gaatcgttct gcttcggctt cagtcacttc tttgactttt
     2461 tgagcttgtg cttcttgttc tttagcgtac cgatcggcat ctgctttttt cttcacttcc
     2521 gaatcatatt gcttttcacg acgtgtgatt tctttttctt ccagttcgat ttgtttttga
     2581 cgttctacga ttttaatctg catttcctgt tcgattactt gttgttgtgc tcgggcactt
     2641 tctaaattgt aggcttggtc tgcttttgct ttagcaacat cttgttcttg tttgtaggtt
     2701 gctaatttca gttctttttc ttttaatgat tctgcgatct ctgtttgtcg ctgtaattca
     2761 gctgcttgtg attctttttc tgcttgagct tttttgatac gcgtttcttt tagagcttcc
     2821 gcttctgcta tatctgcatc tcttttgact tgggcgattc ttggtttccc taaagagtca
     2881 agataaccgt ttttgtctct gacctctttg atagtgaaag aaacgatgat caaccccatt
     2941 tttgcaaggt ctacagaagc gacttcttgt acgctttgac tgaacttatc gcgattttga
     3001 tagatttctt ctacagtcat agaaccaaga atcgagcgca agtgtccttc taatacttct
     3061 cttgcttcat tttccagttc ttctcttgtc ttgcctaaaa actgttctgc tgctgtagca
     3121 atctcttcta cagatgaacc gattttgatg atcgaagtac catcacacat caccggtacg
     3181 ccttgttccg tatacacttc aggggtggag acgtccaatt tactcgataa tagactgatc
     3241 cgattcgaac gttgaaaaac tggcaagacg aatgcgccac ctccacgcac aattttgatt
     3301 ttgttattgc tttcatcaat gtgcacattt ttgcttccta aataacttcc actgatgatc
     3361 aacgcttcat ccggttttgc tgtctggtac ttcgctacaa agacgatcag caacatcagt
     3421 aaaatcaaag ctgcgattgc gatcgcgata aaaacaactg gtatttccat ttaacacacg
     3481 ctcctttaag cttttatcat gctttcataa ggcatgacat aacagattct ttccttgatc
     3541 tcaatgatca atactttcgt tccccgctca gctgtctgtt cttgggggct gtaaaaacaa
     3601 gctggacgac taatggaacc ggtaacactt ttcaactgaa tttctcccat acctcgtaca
     3661 gggatcggtg tgatcacaac agctacttgt ccttccagag ttttttcaga agacgacatc
     3721 gttgattcgg catgtttcat cggcatcaat atatagaaat taagcaaaaa taccaacact
     3781 atcgatatgc ctccactagc aataaataaa aataaagatg attgatctgt taaacgctca
     3841 cctagaaaac caaataagct tgtgaaagtt aaccaaggga taataagcat cggatcaagc
     3901 gggccatcaa aatcgaatac atctccaaaa ataatcaata ggactgccaa tcctgcacaa
     3961 atcagcaatg tataccaata aatcgtctca agtgcgattc cccctatcat atacacgcct
     4021 ccttccattt ctacgtctat cataccaatg tttgaaaatt ctgtatagct tcttttaccg
     4081 actcagtggt gttttttata ggaaggttgt gtttatcgtt gacaatttgt taaatttttg
     4141 ttttcatgtt aaaattttca caataaattc tttcgaggta gcctatgttt ccttttttta
     4201 agaaaaaacc attaaagctc cagaacacga tcatccttgt cgtttgtttc tccacattcg
     4261 tttctttgat tgtttctgcc atattgatca ggaattttat tctttcgatg aaccgatcta
     4321 gtgtgtcaga taaaatctct agcattgcta aaatcgttgc caatgataaa ctcatccaaa
     4381 aaaagctgca gactggtgac ccgacacatg cgattcaagg aataacaaat gaaatcatga
     4441 ttgcatcgaa tttagatttt atcgttgtca tggaccctga aggcattcga tattctcacg
     4501 cagacccaag ttacatcggt cttcctttct caagtcatga agatgtgaaa aaagtgattg
     4561 gaggacaaga atttttctct gaaaaagaag gaatgttagg gttcggcttc cgctatttcg
     4621 tccctattta ttatgatcaa gagattattg gtgcagtttg tgttggctta acaaacaaca
     4681 cgattgacaa tcaagtcaaa gatggacaaa aaatgattgt tttttcattg attattggac
     4741 ttagcgtagg catcataggc gccgtttttc ttgcccaaaa aatgaaaaaa gtactgcttg
     4801 gacttgaacc acaagaaata gcagaacagt taagtgaaaa aacgattatc gaaaatgaag
     4861 tagatgaagg aatcttagcc atctcttctg ataaaactat cctcttgatc aatacacctg
     4921 caaaaaacat tttctaccaa acagagcagc tattggattt cgaagtagga aaaaaatcag
     4981 tgataatcta tatgacgttt tgtttaaaga gacgattgag catcaaacga ccgttcggac
     5041 tcaatccatg tatttaaatg ggatagaaac tatttctagc gttacacctc tttttagcaa
     5101 tgaccgtttt ttaggagcag tcgcaacttt acgaaatcga tcagaagtaa tgaaattaat
     5161 ggaggagctt ggggggacga aacaatatat aaactccatg agagctcaaa cccatgaatt
     5221 catgaataaa cttcatgtca tttctggttt gattgatttg aagaagtatg aagaagtcac
     5281 ccattttatc cagcagctaa atcaaaatta taaaaaagag attggccaag tctcaagtct
     5341 gatcaaagta cctgcaattg ccggtttttt attaggcaaa ataaacgaag ccaaagaaca
     5401 atctgttgaa atgctgttag aaagcgattc ttttatccct aaaatggaga tggatgactc
     5461 catccattca cttttgcaag tgttagggaa cttacttgat aatgcaaaag aagccgtgct
     5521 acaaaaacat gaaagaaacg gacaggtccg gattcattta aactatgaca cagaagctga
     5581 agtattcatc gtgaccgtct gcgatgacgg tgtcgggata tcagaagaaa tctcagaaaa
     5641 aatatttacg ctaggttttt cgacgaaggg aatcaatcgt gggtttggat tgaatctcat
     5701 ccattcaatc gtcaaacgcc acggtggatt tttagaattt tccaaacaaa caaatggcgg
     5761 tacaatcgtc tatctagaat taccaaaaaa ggaagatcaa aatgatgaca aatgtcctga
     5821 taatcgaaga tgatccaatg gttgcagaat tgaatcgtac ttttgtggaa agcatcccta
     5881 atatgaacgt aatcgaaagt ttcagaaatg tagaagaagg aaaaaaatat ttcagacaaa
     5941 aaaacgcaga tctgctgctg cttgatgtat acatgcctgg cgaaagtggt atccattttt
     6001 tatcttggct tagacagcaa ggaaatgata tcccagtgat tttgatcact gctgcagatg
     6061 atatcgaaac cataaagaat gtgatccgct acggtgcctt agattatttg atcaaaccgt
     6121 tcactttgca acgttttcag ctggcgatcg aaaagtatct tgctttctct cagacaatcc
     6181 aaacgactga aaaagccgat cagaaattga tcgatcaagt attcaaccac accactccca
     6241 ttcacgaaat accatcattc atcgatttgc ctaaaggtct gtcaaaactg actttaaaaa
     6301 aaataagtgc atttattcta aaagaagata ctattttttc aacaaaagaa ctggctcaaa
     6361 aaagctcact gtcacgtata tccgtcaaga aatatttgaa ttttttagta gaacaagata
     6421 tattgaacga aagaatcaaa tatttggaaa tcggccggcc gctaacctgt tatgaattga
     6481 caaagaatgg gcggaaaaag ctcacagatt actgttgagt catcgaatta tcccatacaa
     6541 aatagtccaa gtatacagaa gtaacaactg tttacttgga ctatttttaa acattttatt
     6601 gacgaatatc catgttttct tatcttaatt gacgggcttt aggaagagat acctcatttt
     6661 tcgtttttcc agtttctatt atttcacgaa agttctcgaa acttgtcgaa atcatatttg
     6721 ataatgcctc atctgtattt gaacctacat gcggggttac aagaactcta gggtacattt
     6781 ctactaattg ctggatagca gggtctggaa tcgtctccca tggcttgaag gatcggaaaa
     6841 aaacctctgt ttcatttgca aaaacatcgg ttgcaaaacc agctaaatga ttgcttttca
     6901 aagctcttaa aatagcttca ttatcttgaa gctctccacg ggctgtatta atcaagatgc
     6961 tacctttttt catttgagct aaaaatcctt cattgatcat ttgatcgttt tcacctggga
     7021 tatgaggtac atgaatactg acaatatcgc ttttggctaa taactcttca agcgagtgga
     7081 acgtcaatac ttttttagca gcttctgtct gatgaatatc gtagccgatc actttggctc
     7141 ctagtccctt aaacaaaaca gcttccgtca accctatcct gcctgttcct atgatcccta
     7201 ctgtgcaatt tctgatttca cgactaaaca tttgctcatc cactcgaaaa tctttgaaag
     7261 atgttcggat cgtcgtatac gcagtatttc tcaaaagcat catggcaaaa gtcaaagata
     7321 actctgctat cgcatttggc gaatacgatg gtacacgggc tactgtcata ccaaaatcag
     7381 ctgccgcttg tagatcgata tgattgactc ccacagtccg tgtaaaaaca taacgaatcc
     7441 catattcctg catttttgca agattggtct catctgctat acaatttccc ctcaacaata
     7501 cagcatcatg gtcctttgct gacacgatat tgtcatgtgt caacagttct tcataaagac
     7561 tgagatcata atggtaaata ttcaattttt caaaaaaatc tatctcgttc ggacgaacac
     7621 cgtaacatgc gattctcata ctttcttcct ccatttctta tgaaaataaa cctatgccat
     7681 gaatcatttg taatacaacg atccatacag gtgtcagtac cacagcaaaa atagttgaca
     7741 gtaaggatgc attggaggct aaaagtgcct ccctatcaaa actgatagca taggctgctg
     7801 ccactgttgc agctggtgta gccatcatga taacgattgt tgccagtgct tcaaaattga
     7861 ctggtagaat attcgttgcc gtcaaaactg ctagaatcac gatattcaat gcaggtacaa
     7921 ataccacttt gactagactg taataccatg aagttttttc agatgcagct tttttgaagc
     7981 ttacttcacc taatgttgcg ccgattgcta gccacgccaa tggtgaactt aaaccagcta
     8041 aataatccat cggctgaaat agccacggtg cagtctggtc aattcgcaaa aacgctactt
     8101 ggcttatctc tccttctgca cctgtaactg acacttgtgg cagataccct tggaacagcc
     8161 aaatgaaaag gccggcaaaa gtagcgataa tgattggatt gacgaacatc gctttgatat
     8221 ttttagcttc catcttcaag ccactcattt ttatgtatcc ataagaatac aaaaagattc
     8281 ggtagccaat attgaaaatt gaggaataca tcacgccgac aggaccataa atcgcactga
     8341 cgataggcgt tccgaaaaag gtcgtagaac caaagatcgt caggacacgc aacgttgttt
     8401 gcttatcccc tttatagctt aagaaaaaag gttttgaaac tagaataaga ataatataaa
     8461 taactaatcc ccaaatcaag acattgattc cttgcatcag taattctgta ttgatatctt
     8521 gcatgaaagc tcgaaatgcc agtgctggca aagccactgt taagacgact ttcgatagaa
     8581 tttttccaac agattcagaa aaaatcccct tttttctaca aacaaaccct aataatataa
     8641 taaaaactgt tgaagtgatt gcactgacaa tcgacatatc tgtcaaagtc gatcgaaaaa
     8701 tgtcaaataa gttcatcttt ctcctcctaa ttatgttcat ttattcacaa taaaaaaagc
     8761 ttttat
//
LOCUS       DANKHL010000120         8729 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000120 DANKHL010000000
VERSION     DANKHL010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.124"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(70..903)
                     /locus_tag="PW101_001953"
     CDS             complement(70..903)
                     /locus_tag="PW101_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDL2514348.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(1207..3372)
                     /locus_tag="PW101_001954"
     CDS             complement(1207..3372)
                     /locus_tag="PW101_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2514349.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            3632..5926
                     /locus_tag="PW101_001955"
     CDS             3632..5926
                     /locus_tag="PW101_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL2514350.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5919..6584
                     /gene="pgmB"
                     /locus_tag="PW101_001956"
     CDS             5919..6584
                     /gene="pgmB"
                     /locus_tag="PW101_001956"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL2514351.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            6577..7584
                     /locus_tag="PW101_001957"
     CDS             6577..7584
                     /locus_tag="PW101_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL2514352.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            7606..8625
                     /locus_tag="PW101_001958"
     CDS             7606..8625
                     /locus_tag="PW101_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2514353.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
BASE COUNT         2668 a         1893 c         1608 g         2560 t
ORIGIN      
        1 tctccaaacg gtataatcgt ttggagagtt ttattttaat cagttttttt atgttatatg
       61 ttatattttt tattcgttca catctaattc gatttccacc ccgtagtgat cactgatgat
      121 tggactatcg atcccatcaa aaatccgttg atatttttta acctgcattc cttcagggac
      181 gaaaataaaa tctatacgca atttttctgt attttgttcc caaccatcta ttttcttttc
      241 cacagtagct gcttttcctt tttcattcgc gacaacatac gaatcttgaa gaagaaggca
      301 actttctctt acagtataat aaccttgaga gtctactgga ttattgaaat cacctaggaa
      361 aaaaagtggt acgcgagttt ccaaaaagta gttttccaag tttttccact caaaataaaa
      421 gccacttttt ttatctgtcc accaagaaaa gtggcagctc gagacaaaaa tgtcctgatc
      481 atctatcttt gttttcccaa tcaagatctt gcgtgtctga taatcttctt tcttttgaaa
      541 ttgggagacg gtaaggacct gactttccaa tgtttttttc gtaagcagtg catttccttc
      601 ttcatattta tcataaccga tgtggctcat ttcccaactc caaaaatact cttttccgtg
      661 ttcctttaaa agctgaacta aacggtaggc aaaattatct ttacggatag gtacttgatt
      721 ctttaccgga cagaacttca ttagcttcgc aggttctact tcttcagatt ccagtaattg
      781 gttgatttct tgtaaagcaa tcacttcata atcttctaac aggatcttat ctgctaattc
      841 ttgcaatttt ttctctggtt ctggctcaag ccagctatgc gtgttgagtg tcatgaattt
      901 cattggtttt ctccttgtct tcatttcgaa acaaaagggt tgtgacaaaa tttttgtcac
      961 aacctctcca gcaaatagtg ttctcctgct aactcctcag taataagctc aatggaccag
     1021 gagctatttt ttgtttggct gtttttatca cttggtgcag ggcagctttt tcacgacctc
     1081 tccactaaac agtattcaaa ctgctgcttc tcggtaataa gcccaaccaa acgaaaaata
     1141 ggaggagcta tttttgtttg gctgttctta tgacttgatg caggacagct ttttcacaac
     1201 ctctccttac attttaatgg agccgatcac tttaccagct gtcactgtac ctaattgctt
     1261 gatcactact tcttctactt tatcgtttgt aaatacgatc atgatcgttg gatctttttc
     1321 ttcgttttgg atcacatcaa gatccatctc agctaatttc gttcctgttc ctacttcttg
     1381 gccttctttg acagcaatct caaaagctgg ctgattcatt tggactgtat cgattcccat
     1441 atggatcaaa acttcgatac catctttcgt ttccagccca attgcatgtt tgctagggaa
     1501 aatacttgtc acttttccag cgatcggcgc aacaacttct cttgatgttg gaataacagc
     1561 aaagccgtca cccatcattt tttgtgagaa taccggatct tttacttcag ataaaggaat
     1621 cacttctcca gaagctgcgg aaactagttc ttgttctata cctaagtatt gcttactacc
     1681 tgcttgtcca tttcctgtag aaaccatttc tggctctgga atctctgctc ctgattgcag
     1741 cagatcttcg atatctgatt tcaagacatc tgcttttggt ccataaactg cttggactcc
     1801 attgtctttg acgatcaatc ccatcgcccc tgctcgtttc caggcttctt cagtccctac
     1861 tttttctcga tcatttacac ttacgcgtaa ccgagtcata caagcatcga catctttgat
     1921 attttctttt cctcccagca aatgaatgat ctggaccact tgctgatctg ctgttcctgt
     1981 tccgctgtta gatacaacgg tttcttcact gtcattgtcg taatttccgt tacgtcccgg
     2041 tgtagcaaag ttgaattttt tgatcaagaa atttgctaag aaatacgtaa caacaccaaa
     2101 agcgatcaca cacaacacga agttgataag atctccacct agacccgctt tgatagctaa
     2161 cggtgtccga gtcagcaatt cgatattccc aaatgaatgg acccgcaatg gcaagatatc
     2221 tgccatcgca aatgcagccc cttggatcac tgaataaatg acatataatg gcacagcagc
     2281 aaacataaac atgaactcca atggttctgt tacacctgtt agaaagacag ccaacgctgc
     2341 tgaaaaatac attgatttat atcttgcttt cttatcagga tcgacattac gatacatcgc
     2401 aaaagccatt cccatcaaga tacctgaaga accaatcatt tgtcctactt tgaaacgagc
     2461 cggcgtccag ttatgcaaca caaactcata ttgcgacata tcccctgcac ctttaagatt
     2521 cactaaatca gtggcccaag ccaaccataa cggatcttgc ccaaatactt gtgtccccgc
     2581 ctgtgcgcct gataaaatct catacgatcc gccaagctgt gtatagttga taggaatcgt
     2641 cagcatatga tgcaatccaa atggcaatag taaacgttct aatgttccaa ataaaaacgg
     2701 tgctagaatc ggtgcgcttt cttgtgattc tgcaatccat aaaccaaaat tattgattcc
     2761 agcctgtacg tttggccaca ccacagcaag aatcaatgcc acgattgttg accataagat
     2821 aacaacaaac ggaacaaagc gctttccatt gaaaaatgac aaagcatccg gcaattttct
     2881 ataattatag tacttattat aagccattgc cccgacaaat ccagcgatga tccctacgaa
     2941 tactcccata ttcaaagctg gtgcttccaa tacacttgta aaaaatcctt taaccatgat
     3001 cttcgtaccg aacagagtgt gggtaaatgc atcttcattc gcaagcatgt cacttgtgac
     3061 accaaagata gccccagtga tccgattgat taaaataaat gagatcccag ctgcaaatgc
     3121 tccgcctgct ctttcttttg cccaacttcc tcctattgct aaagcaaata aaatatgaag
     3181 attaccaata attgcccaac caatattttc aattactccg cccgttgtga caagcggcgt
     3241 ccaatcagga ttgatcaagg gaattgattt ccctatactg atcatcaacc cagctgccgg
     3301 cataactgct accacaacca ttaaagcctt tccaaatttt tgccaaaact caaaactaaa
     3361 tatctttttc attccgttta gctcctttac gcaatcggtt tcgttaaacg tattatagcg
     3421 tgcgttttct tttttgtaaa tgctttattt tttatttttc tgtctatacc attctataaa
     3481 acctataata cgtgtgttcc cttctataat tttctcctaa aaatttattt ttttaagctt
     3541 ttttctatga aatattttct tttgtctatt gacttgtgca atcggttgcg tttattatga
     3601 acgtataaaa cagctcatta ggaggagaaa aatgaaacag atcaaacgac tatttgctat
     3661 tgatccatgg aaaatcagca cgacacaatt agataaaaaa aatcttcgac tgcaagaatc
     3721 tttaacaagt attggaaacg gttatatggg gatgcgcggg aactttgaag aaacttactc
     3781 aggggaccac catcaaggta cttatttggc gggtgtctgg tatcccgata agacacgtgt
     3841 cggctggtgg aaaaatggtt atccagaata ctttggaaaa gtgatcaacg ccttgaactt
     3901 tatcgcgata gatatttcga tcaacgatca gctgatcgac ttggcttctt tggaaccaga
     3961 agacttttac tgggaattag atatgaaaaa tggtgtatta tctcgtacat tcacaatcac
     4021 tacttctact aataaagtac gcatttcctt cgaacgtttt ctgagcattg tcaaaaaaga
     4081 atcagcttat atctatttaa ctatagaaat gcttgaaggt agcggaacag tcaaactcgt
     4141 atcaaaattg gatggaaatg ttcaaaacga agacagcaac tatgatgaac atttttggaa
     4201 tgagattgat cgtggaacta tagaagaaat cggctatttg acgactaaaa ctattccaaa
     4261 caactttggg attgaacaat ttactgttac tgcagcaatg cgccatttcg ttaatggaaa
     4321 atcagtcatc cctgactatc acgaagatac tttagcatta catggttgct tgtctgcaga
     4381 attatctcaa ggtgagacat ggtcagtgga aaaagaaatt atcgttctta ctagtagaga
     4441 tattccagaa gcgcagcaag cagaaaaagc tgtctcagaa ttaacgaacc agacaaacaa
     4501 ttatatccaa gcaaaagaag agcaaacagc ggcttgggca gaacgatgga agcttgccga
     4561 tgtggtcatc gaaggagacg aagaagcaca acaaggtatt cgattcaatt tgttccaact
     4621 attctctact tactacggcg aagatgaacg attgaatatc ggccccaaag gattcacagg
     4681 agaaaaatac ggcggagcca cttactggga tacagaagcg tatgctgtcc ctctttatct
     4741 tgctttagcc aaacctgaag taactaagaa tctgctgaaa tatcggcata atcaactgcc
     4801 gcaagcaatc cacaacgctc agcaacaagg attaaaaggc gcgctttatc ctatggtcac
     4861 cttcactggt gtcgaatgcc ataacgagtg ggaaatcaca ttcgaggaga ttcaccgaaa
     4921 tggcgcgatt gcctatgcaa tctacaatta tacgaattac accggcgata cggaatattt
     4981 gaaaaatgaa ggactggaag ttttagcaga aatcgcacgc ttctgggcag accgtgtcca
     5041 tttttcaaaa cgagcaaata aatacatgat ccacggtgtg accggcccaa acgaatatga
     5101 gaacaatatc aataacaatt ggtatacaaa tacgatcgca gcttgggtct tgcgttacac
     5161 gcgagaaaat tatttaactt atcaaaaaga agctgctatc actatcagcg aagaagaact
     5221 agccaaatgg aaagatatcg cagagaacat gtacttccca gttgacgaag aattaggaat
     5281 ctttgtccag catgacacat tcttagataa agatctcatg cctgtatccg atattcccac
     5341 aagcgatctt cctctgaatc agcattggtc ttgggacaaa atccttagat cttgcttcat
     5401 caagcaggca gatgtactac aggggattta tttcttcaat gatactttta caaaagaaga
     5461 aaagcaaaaa aactttgatt tctatgaacc aatgactgtc catgagtctt ccctttcgcc
     5521 aagtatccat tcgattttag cagcagaatt aggaatggaa agcaaagctg tagaaatgta
     5581 ccagcgaaca gctcgcttgg atttggataa ttacaataac gacacagaag acggcttgca
     5641 catcacttcc atgactggta gctggttgtc gattgtccaa ggatttgctc aaatgaaaac
     5701 tgcccatgat cagttgagat tcgcaccatt tctgccaaaa acttggaaag cttattcttt
     5761 ccatatcaat tatcgcggac gtttattgtt catctcagtc acagatgaac aagttacttt
     5821 cgagttatta gaaggttctc ctctagaaat ttacgtctat gaacagtctt attctttagc
     5881 ggatcgtcta gaaataacgc taagaaagga ggaagcaaat gtttaaaggg gtattgtttg
     5941 atttagacgg tgtcattacc gatactgctg aattccatta tcgtgcttgg cgaaagcttg
     6001 gtcaggaaat cggcattacg atcgaccaca cattcaacga acagctaaaa ggcgtcagcc
     6061 gagaagactc actctctttg cttttagctt atggcggaaa agaacactct ttttcaaagg
     6121 aagaatttgc agaattagcg aaaagaaaaa acgattatta tttagagatg atccagacga
     6181 tcgaaccaaa agatgtcttc cccggggtcg tgcctttact ggattcttta aaagaagaaa
     6241 aaatcaagat tgctcttgct tctgcaagta aaaacgggcc attcctactt gaaaaaatgg
     6301 gtcttacacc ttactttgat gctattgccg acccggcaga agcaacaaac ggaaaacctg
     6361 cgccagatat cttcctcctt gcagcaaaag ctgtcggact aaaagcagaa gaatgtatcg
     6421 ggatcgaaga cgcgcaagcc ggtatccaag ccatcctctc aagtggcgct caaccaattg
     6481 gcgtgggacg tgctgaagac cttggagagg agatcccgct tgtccctgat acgacttttc
     6541 ttacgatcga ttacttgaaa gaaaagtggc atgaccatgg ataatccaga gatctccgtc
     6601 gtaccagttc cagagactga aataaagaaa atcacattga agaacgacta cttagaagtc
     6661 gttcttctca attacggtgc ccgcctgcac caaatcttcg caccagacaa agaaggaaaa
     6721 tcagaaaatg ttttattgtc ttacgatcgt ttcgaagatg ttctaacaga caaaagcttt
     6781 tttggagcaa ctgtcggacc tgtagccgga aggatccggg atgcttcttg gggtgagcat
     6841 agattggaga aaaactgcgg atcacaccat atccacggtg gcacgaaagg ctggtctttc
     6901 caattttggg aagtagaagt tttcaaaact ccatcatcaa taggtgtggt cttttactta
     6961 aaagatgaat tttctaccta tccaggacca atcactgcta cgatcactta ccgtttaaca
     7021 aaaaacgaat tagagatgac aacctctgct agtagccttg ttgaaaccat ctgcaatccg
     7081 accaaccatg cctacttcaa cctttcagga aatggaacaa gagacatcta tgaacatcag
     7141 ttaacggtct ttttagatgg tgtgcttgaa ttagatcaag aaaaactgcc tactggaaat
     7201 tggacaaaaa aagaaaaact gccaatcgac tttagaaaat ccccaacact gcaagagatc
     7261 cttgcatgtt atccagatgg actagatgat gtatttcttt tgcatcatcc taggctttcc
     7321 aaaacttcct tacaattatt cgagaagcat tctggtcgcc aaatgactat agcaacttct
     7381 aataaaagta tggtattatt ttctacgact ggattcgaag ctgatttctc tgtcaatgga
     7441 aaacagatgc attcaaacta cggattagca atcgaaccgc aagaaatccc tgatatcgtt
     7501 catttcccca aatttggatc gatcaacctc catcctggtc aagaacggat cagtcagacg
     7561 atttatcggt tctctgcaca ataacaagag aaaggtgttt ttatcatggc tattaccgta
     7621 aaagatgtgg cgaaaaaagc cggtgttgcc acctcgaccg tctctcgtgt catcaacgat
     7681 catcctagta tttccgagac aacaaagaag aaagtccgaa aaattatgga tgaactaggt
     7741 tatgttccta acatgactgc ccgcaatcta ggaaaacgca tttccagtgc catcggcgta
     7801 atcttacccc ctcttgattc aaaagagcgg atcggtaacc cattttatct tgagatcatg
     7861 gaagcaatca acgaagaagc gcggaattat gatatgacta cagcaatcgc tactgctaaa
     7921 aattttgaat tgctgttaga gaacgtccag cgtatgcatt tacaaaaaca agtagatggt
     7981 tttattctag tgtattcaga tagtcaagat actgtcattg attatttata ccaaaaccac
     8041 atccctttca ctttgatcgg acagccttat catcatgaga acgaaatcgt ttatgtagat
     8101 aatgataacc agcttctagg taaacaagca acagaatttt tgatccagaa tggacataag
     8161 aatatcctgt ttgtgacaaa cacgactcat gaaagtttgt atttcgaacg atatttcggt
     8221 tatcaaaaag caatgatgat ggctggtctt cctgcgttat ccagtgtagt actagagcat
     8281 ccggaggatt atctgtcctt tgagaaaacg ttgaaagaaa acgaagccac agcattggtg
     8341 gtcattgacg atatcttcgc ccttcgcacg atgcagttgg cgcaaatgta tggctatgtc
     8401 attccagaga cactctccgt cattagcttc aacaactcga tcttctcaac attaacacac
     8461 ccttacctta caagtatcga tatcgatatc tccgaattag gacgtgtcgc tatgcaaaag
     8521 ctgcgcgaat tgatccatga agaattgagc agtggcgtac gtttggtcat ccctcatcgc
     8581 ttgatcaaaa gagagacagt cgttccttta gaaaacccat actaaatagc tgactcacta
     8641 aatacgctga ccatttatcc tatggtcagc tttttatgta aaagaagata ctacttctaa
     8701 cacattgttt atctgtaaaa aagagcgtg
//
LOCUS       DANKHL010000121         8551 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000121 DANKHL010000000
VERSION     DANKHL010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.125"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            24..1397
                     /locus_tag="PW101_001959"
     CDS             24..1397
                     /locus_tag="PW101_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL2514354.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            1397..2323
                     /gene="dnaI"
                     /locus_tag="PW101_001960"
     CDS             1397..2323
                     /gene="dnaI"
                     /locus_tag="PW101_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDL2514355.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            2452..2892
                     /locus_tag="PW101_001961"
     CDS             2452..2892
                     /locus_tag="PW101_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL2514356.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            3036..3737
                     /locus_tag="PW101_001962"
     CDS             3036..3737
                     /locus_tag="PW101_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDL2514357.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            3734..4855
                     /locus_tag="PW101_001963"
     CDS             3734..4855
                     /locus_tag="PW101_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDL2514358.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            4870..6102
                     /gene="pepT"
                     /locus_tag="PW101_001964"
     CDS             4870..6102
                     /gene="pepT"
                     /locus_tag="PW101_001964"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDL2514359.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            6234..7142
                     /locus_tag="PW101_001965"
     CDS             6234..7142
                     /locus_tag="PW101_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDL2514360.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            7177..7449
                     /locus_tag="PW101_001966"
     CDS             7177..7449
                     /locus_tag="PW101_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDL2514361.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            7460..8506
                     /locus_tag="PW101_001967"
     CDS             7460..8506
                     /locus_tag="PW101_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDL2514362.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         2872 a         1489 c         1863 g         2327 t
ORIGIN      
        1 agaaacaaaa aggaggcatt tccttggaaa atgcttggaa agagcttcaa ccaaaaaata
       61 tttaccaagg aagaaaagcg gctccgctga gagaagacag caagaacgcc ttgctgtatc
      121 tttatcagcc actaatcggc ggagaagcct tgagtatcta tttcaccctg ttatcagaaa
      181 tctctcaaga atctggcaaa ggtcctgaag ggatgcatgc agatctgctt agtagtctag
      241 gctgcggtat cccgcaattt tatgatgcaa gaaaaaagct agaaggaatc ggcttactgg
      301 atgtttattt taaagaagat agtgatctgg gacctcgttt tatttatgag ctgttagaac
      361 cactcattcc taaagcattt ttcaaagact ctgttctggc ttttcttctt ctggaaaaag
      421 ttggtgaacg acgattccag aagcttaccc aattctttga acctaaaagg catacagcag
      481 agggatatca aaaaatcaca aaaaaatttt tagatgttta tcgcttcaat gaagcagtgt
      541 acgctgctaa tcaagaaaac atcgaatcgc tgaataaatc ttttgaagaa tctgaatcag
      601 tagcattact tgaagcagat tcgacattcg attggcagtt tttgaatgat tggctggcaa
      661 aaaaacatat tgcaagtctt gatgatccaa tcatcaaaca agtcgacatg tatcatcagc
      721 tttacggttt cgatgaattg acacttggcg aactgatcag tcaatcttat gactttagtg
      781 aacaaaaagt atctgaaaaa gaactgcaaa aagccatttt ggcaatgcag agacgtccgg
      841 tgaagcaaac aaaagaaaca gttgcggttt ctgaaaacag taatgaaccc tcgtcaaacg
      901 aacatttgac aaacgcagcg cttcagctga taaaagaagc acagagcatt ccaccgatga
      961 aatatctgga agcaatcaag tcagaaaaag gcggatatgt ttctaaacaa gaaaactggt
     1021 tactgcaaga tcttgtttct caatcaggtc tttccagtag cgtgatcaat gttttgctga
     1081 attatgtact agtgatcaag aataatgcta gcttaaatgc cagttatgta aatacagtgg
     1141 caaatgaatg ggcacaaaag aaaatcgcca cagcagaaga agcgatccag cacattcggc
     1201 aaataagtaa tcaagcaaaa caatcaaaac aaaagaagca aacaaattat caaactggca
     1261 agcgaaatgt tcgacgggaa aagcttcctg attgggtcaa ccagccaaaa gatgaaaaac
     1321 agatcagcag cgagaaaaaa gcagagattg accgccgttt taaagaatac ctggcaaaaa
     1381 aggagggtga tatctagtgg aagacgtagg caaagaattg aagaggatca tcagcaaacg
     1441 caattatcaa gaaaagtatg ctgaaatgat cgctgaagtc ttaaatgatg cggatgttca
     1501 agcttttcta agcgcacacc aagaagaatt gacacaagcg gatattgaaa aaagttacgc
     1561 taaattgtat gaattcgtgc aagaaaagcg taaatatcaa aaaaacgatc caacgatgat
     1621 cgctcccggc tatgagccga agctgacgtt gaattttcat tatgtggatg tcacttatgt
     1681 accaacggaa gcattgttag cacggcaaag acaagaagaa atcagaagcc gagtaaaagc
     1741 aatggatatg ccaaaagata ttcaatcggc ttcttttgaa aactttgaac gtacagatgg
     1801 acgaggatat gcgagcttag aagccctaga ttttgtagag aaatatactg ctgatccaaa
     1861 agggtatcat aaaggactgt atctagtagg aagttttggt attggaaaaa catatttatt
     1921 aggagcaaca gcgagagaat tagcgattgc tggattcacc acgacgctag tccattttcc
     1981 gacatttgcc gttgagatga aacaggcaat cggaaaagat caagtggcag aaaagttaga
     2041 tgcagtgaaa cgttcaccta ttttgatgat cgatgacatt ggtgcagatg cgatgagtag
     2101 ttggatacga gacgaagtat tcggtgttat ccttcaatat cggatgcagg aacagttacc
     2161 aaccttcttc tcatcgaatt ttacaatgga tgaattggaa cagcatttgt ccgtcactca
     2221 acgtggagat gaagagccgc taaaagcgaa acggataatg gaacgcatac gttatttgac
     2281 aaaagaaatc gaaatgactg gtcgcaaccg acgcaatggt tgaacagttg acaagtccaa
     2341 ctgacatttg acctagtagt aaaagcaggt cagcgaatca ttatatggac agaaatgacc
     2401 ggagacaatc accgcaacaa gtaatgcaga aacttaaacg gaggattttt tatgagggct
     2461 attttttcta gtatatctgt agttggtcta ttttttcttt tagcaggctg cggtagcgca
     2521 gatagcaaca caggttctgt tgattcgatt gatgcgggtc aaacagtaaa aacaagcagt
     2581 acgaaaacgg agagacaaag catatttgtc ggaacgattg aaagtatcac tcaagtagaa
     2641 gcagaagaag aaacgcttca gttgaaactc gtcgatgtca tggccaaaga agatccagcg
     2701 aatatgggaa cgtcattcag caatgacgga gtgatcttaa atgctactgc tgaaaaaata
     2761 agcgtggaca taaatgaatt gaaaatcggt gacaaagtcc aatttacatt agttgagatg
     2821 ccgatcatga cgatgtctat cccaccgcaa gttcctggag attcgattat tgatgtttca
     2881 ttgaacaaat aaccaataac attttttcaa aaataagaaa atgttgtttt atagaacgaa
     2941 aaggtatatt ttttaaggag atatgggaat ttttttattc ctagtctcct tattttattt
     3001 ggaaaatgct ataattatct agaaggagtg aagctatgaa ccatttagat ttatctcagc
     3061 gcttagccgc agtgggtgac tttgtccctg aatccgctcg tttagcagac attggatcag
     3121 atcacgctta cttacccgtt gcattgatat taagaggaaa aatcgaatat gcagttgctg
     3181 gtgaagtcgt tcaaggtcct tatgaatcag cacgtaagca agtcaaaaaa gatggattag
     3241 aagaccgtat caccgtacgc cttgcaaatg gtctagatgc tatccagcca caagatggta
     3301 ttacagctgt cacgattgcg ggtatgggag gttcactgat ccgagatatc ctagaagctg
     3361 gcaagttgaa tcaacgctta agcggacaag aacgtttgat ccttcagcca aacatcggag
     3421 agcgtacatt acgatcttgg ctgcaagaaa atcagtataa aatccaatca gaaaaaatac
     3481 ttgaagaaaa taaaaaaata tatgaaatca ttgtagccga aaaagaacaa aaaccagtta
     3541 cttacaatga aatggagctg atgttcgggc catttctgct tgaagagaaa agtgagatct
     3601 tcaaatcaaa atggacaaga gagcttcaac aaagaaaagc tatccgtgaa cagctcaaac
     3661 aagcagctaa tcaagaggca cgtcaaaaac aaatcgagca ggaaatccaa atgattgagg
     3721 aggttttaaa gccatgacaa tcagcggacg cactttcatc caaaagtttg aaagctattg
     3781 tcctcagtgg ctagctgaag aaggagaccc agtcggactg catatcggaa cattggataa
     3841 gccaatccaa cgagtaatga tgacattaga cgttcgtcca gaagtggtcg aagaagcaat
     3901 caagaaaaaa atcgatttgc tgattgctaa acatcctcca atctttcgtc ctgtaaaacg
     3961 gctggttact gatcagccac aagaaaaaat gtatgcagat ttgttgaaac atgatattgc
     4021 cgtctatgca gcacacacga acatggatat tatttgggat ggactgaatg actggttttg
     4081 tgaacttctc ggtattgaag tggagagtta tttagtaaag actcatgaga ttcattataa
     4141 aaaactggcc gtttatgtac ctgtggatca tgcacagaaa atgcgtgaag tattagctgc
     4201 agctggtgct ggtacacaag gtgattatac cggtacaagt tttacctcga ttggacacgg
     4261 tcgatttact ccggaagctg gtgcacaacc agcgattggg aaagtaggga agacagaaca
     4321 agttcaagag gcaaaagttg aggttatttt gccggaaact atcgaaaaac aagtgattca
     4381 agccatgcgg tcagctcatc cttatgagga acctgcttat gatcttttcg ctatagatga
     4441 accagtcgaa atgtttgggt taggacgagt aggtgaattg ccgcaagaaa tttctataga
     4501 ggcatttgta gaacaagtaa aagaagcgtt ccaactagat ggactgagaa tagtccagcc
     4561 aaaaaatgca aaatcctctg tgaagcgcat tgcgatttgc ggtggaagcg gtgaaaaatt
     4621 ttatccgcaa gcaatcgccc aaagagcaga tgtctatatc acgggagata tctattatca
     4681 tacggctcat gatatgcaaa gcgctgggtt gattgcaatt gatcccggtc attatattga
     4741 atcgttatgc aagcaacgat ttatcgagaa atttgaatct tggaaacaag aagaaaattg
     4801 ggatattaac tttttcgttt ctgaaacaaa tacaaaccca tttcaattta attaaaagga
     4861 gagaatacta tgtacgaaaa tcttttgcca cgttttttac gatatgtcaa aacagaaaca
     4921 cgctccgatg caacaagcaa aacgactcca tcgactcaga cacaagttgc ttttgcgcaa
     4981 gaattgaaaa aagaattaga agagttaggc ttagctgatg ttttctataa tgaagataac
     5041 ggattcgtta tgggaacatt accaagcaat gttgatcatg aagtgcgttc cattggattt
     5101 atttcccaca tggatacagc agattttaat gcagtaggag tcaatccaca aatcattgaa
     5161 aactatgatg gcgagtctac gatcgtacta gatgcggaag aacgctatac attgaataca
     5221 aaagattttc caaatttgaa aaattatgaa ggccacacat tgatcacaac agatggcagt
     5281 acattactgg gagcagatga caaatcaggg attgcggaaa tcatgactgc aatggagatc
     5341 ttgatcaata atccttctat ccctcacggt accatcaaag tggcttttgg tcctgatgaa
     5401 gaaatcggtg tcggagcgga taaattcgat gttgagcaat tcgatgtaga ttttgcttat
     5461 acagtcgatg gtggtccttt aggtgagttg cagtatgaaa cgtttagtgc tgctcaagca
     5521 aatattacga tccaagggaa aaatgtacat ccagggacgg caaaagatac aatgatcaat
     5581 gctctacagt tagcaatcga ttttcataat cagcttccag ccgatgaagt tccagaaaaa
     5641 acggaaggat acgaaggttt cttccatttg atggcattgg atgggaatcc tgaagaagct
     5701 tctatgagtt atatcattcg cgatcatgat cgtgaaaaat tcgaagcacg caaagcacaa
     5761 atcacacata tccaagaaga gttgaatcag cgatttgatc aagaacgaat caaagtagat
     5821 atgtatgatc aatattacaa catgaaagaa atcattgaaa aagatatgag catcgtggaa
     5881 ttagcgaaac aagcaatgat cgagcttggt atctctccag tgatcgaacc agttcgtgga
     5941 ggaacagatg gttcaaaaat ctcttacatg ggtattccga cgccaaatct atttgccgga
     6001 ggagaaaata tgcacggacg attcgaattt gtttctttac aagtaatgga aaaagcaaca
     6061 gacgtgattg taaaaattgc agaattgaac acaaagagtt aaaatcgaaa aagaacagct
     6121 tatgacaagg cgttcgtccc aatgatggaa tgtcatctgt ctaaggctaa agggcaggac
     6181 aatggaattg gttgtcccgc cctctttttt gaagagtaaa taggaggtaa agaatggaaa
     6241 agctgctagt tttttataac aaaagttcag ggaaagatga gggtgagaaa ttagcacgct
     6301 ggtttcaaga ttatgtaaaa gaaaagcagc cagaattata tgtagaacta gctgaaactg
     6361 gcccatcgat tgatgatgcg gaattagtca agaaagcaga agccatagaa gctgatactt
     6421 tagtaattat aggtggagac ggcacgattc atcatatcgt ccaagcgttc caaaaaacat
     6481 tatctcaata ccatgtcgga ttacttccag gtggaactgt caacaacctt gcacgtgttc
     6541 ttgagattcc cctcaaaaaa gaagaagctg ctgacgtgat cctggaaggc aatgtacgga
     6601 aaatagacta tggtcaagtc aatgatgaag tcattatctc tacactgaca atcggaatct
     6661 tagcagatac agcagtttgg atcagtcaag aagaaaaaca gcgttacggc tcgtggatct
     6721 ttatcaagaa gttttttaag ttattgctga agaagaaaaa atatcatttg gatttagaaa
     6781 cagacgagga gcattggaaa ggaaaatccc aactgttgac agtcacgatg tctaactccg
     6841 tgggcggttt tacaaatttt gacgactcag cgaccctaga tgatggaaaa tttcatgtaa
     6901 cgatcgtacc ttccttagat attttccgat ttatttttta tttgcctaga atcatgagag
     6961 gcaaattcta tacgatcccc ggcattactt atttcacagc caaacgcatg aaaatccaag
     7021 cgcaggaaaa atctgtgggc actcgaacgg atggagacac gacagatgat cttccagttg
     7081 aattatctgt tgtatcaaaa ggattaacag tttattcacc aaagaaagaa gaaaaagctt
     7141 agaaatagta gttaacagaa agttggaaga agagtaatgg atgtattgtt acacaatcct
     7201 ttgtggtttt accttttcat tgtgaatttt tacttatttt gtctgatggg gtatgataaa
     7261 catcaggcta gaactagcgg ctggcgtgtt cctgagaata atttgatatt tgtcagcctg
     7321 ttaggcggtg gtttgggagg attgatctct cgaaaagttt tccatcataa aactcggaaa
     7381 aagaagttta ctataggatt cgctgcaggt gtacttgttg atttgattct catttatttt
     7441 ttccattaag gagcaggaga tgtcgtctaa aggaacgaaa actaaaatca ttctgaatct
     7501 gcttttgctg attgtgattt ttgccattat ttattattta atcaatcaat catttgcaga
     7561 tatctttgcc gaattgctgt ctaccagtgt tgttgttttg gcagcggctg ttatattagg
     7621 gacagtttac caaatcgctg aagggcgctc aatcaaagaa attgctgaac ctttcgcgcc
     7681 gaattttaca acgaaagatg ggttcttgac ctctagctat attgcgtttt accgaatcgt
     7741 ttcgtttggt acaggaacgc tgatctcgga gatttatttt tataacaaaa aaggaatgaa
     7801 gctatcccag ggagcagggg tgacagcact ccatatgatt atgtacaaac tggcagtgat
     7861 cacttatgca ttagctggct tggtcatcca attctctctt ttttattcta atggtccatc
     7921 aatgatctgg tttatcatcg ctggtatcct tttgacgatc gtgattgttg cctttttatt
     7981 ggcgatgtcc gtcagcttga atgttcaaat attttttgtc agcttatcga ataagttatt
     8041 taaaagtgag cgtttgcgga aatgggtgga tacatgcaat cagcagatct actctttaag
     8101 ggaaacggtc caaaccatta tccaagacag gactgcgcta ttgcggattt atctatggaa
     8161 tatgttaaaa cttgtatttt ggtatgtact tccttatttg attctgattg aaaaccatcc
     8221 taaaatagat tttctactga ccatttcgtt tatcagcttt gcagtagtgc tttcaggggt
     8281 tattcctacg cctgctggta ttggttcctt tgaatttgtc tatttgttat tattcagacc
     8341 agttgttgga acagttgacg cagtttcttc gcttttactg tatcgtttca gttcctttat
     8401 cttgccattt ctctatggat ttgtgtatgt gattgctgaa cggcgaagag tgatcaaaga
     8461 agaaataata gaagtaaaaa aagagaataa agaaaatcaa gggtaggtct tttataagat
     8521 ctaccctttt tatcaaatca aagaaaaacg t
//
LOCUS       DANKHL010000122         8528 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000122 DANKHL010000000
VERSION     DANKHL010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.126"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            257..1780
                     /locus_tag="PW101_001968"
     CDS             257..1780
                     /locus_tag="PW101_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDL2514363.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            2017..3357
                     /locus_tag="PW101_001969"
     CDS             2017..3357
                     /locus_tag="PW101_001969"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDL2514364.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            3510..4247
                     /locus_tag="PW101_001970"
     CDS             3510..4247
                     /locus_tag="PW101_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HDL2514365.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(4412..4633)
                     /locus_tag="PW101_001971"
     CDS             complement(4412..4633)
                     /locus_tag="PW101_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDL2514366.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            4664..5695
                     /locus_tag="PW101_001972"
     CDS             4664..5695
                     /locus_tag="PW101_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2514367.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            5682..7085
                     /locus_tag="PW101_001973"
     CDS             5682..7085
                     /locus_tag="PW101_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDL2514368.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            7151..7786
                     /locus_tag="PW101_001974"
     CDS             7151..7786
                     /locus_tag="PW101_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2514369.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            7835..8515
                     /locus_tag="PW101_001975"
     CDS             7835..8515
                     /locus_tag="PW101_001975"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDL2514370.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
BASE COUNT         2824 a         1449 c         1820 g         2435 t
ORIGIN      
        1 atcaattctt tgcaaatgtg aaattcttct ccttttaaaa gacgaaaatg tttgtttata
       61 aaaaaaagga aacgctacaa tgaaaaagaa gaaagcttta aaaacctgaa aaaagaggag
      121 ggaataggga aaaacgaact ttttgaacaa aagttttaca aaatgcgtgt tattattttt
      181 gttctgatac tctttttgat aaaataacat tagagccaaa attgttgtct ctaacggaaa
      241 agaaaggaag aagaagatgg aacaaacaaa aaagaaaaaa tttcaaatgc cttcagcgta
      301 tacggtctta tttattatta ttgcagtaat cgctgtactt acttggttta taccagctgg
      361 gcactatagt gtagacgatg caggcaacat cgtcgctgga tcatatgaac gagtacaaca
      421 aaatcctcaa gggctgtggg atattttcat ggcgccaatc aatgggatgc taggtgtacc
      481 agcgggggag ctaactaaag aaacacctgc tgcaatccca atctctttct tcattttggt
      541 tgttggtgga tttttaggta ttatcaacaa aacaggggca ctagatgccg ggattgcttc
      601 agttgtgaag aaattcaaag gaaaagaaaa aatgcttatt ccagtattga tgctgttatt
      661 tgcattaggc ggctctactt acggaatggc agaagaaacg attccattct atgctttact
      721 gatcccagtc atgatggctg taggctttga tacagtagta gctgtagcaa tcgtattagt
      781 agggtctcaa gtaggctgtc tagcttcaac ggtaaatcct tttgcaacag gcgtagcttc
      841 acaagctgtt gggatttcga tgggtgaagg tattggtctt cgattcttga tgttcgttat
      901 tttgttagct atttctattg tatatgtgta tcgttatgca tcaaagatcg aaaaagatcc
      961 aacaaaatca ttagtataca accaacgtaa agaagatatg aagcatttcg atattgaagc
     1021 aatcggtcgt caagaagtaa tcacgaaaaa acaaaaacat gttaatgtat tgttctttat
     1081 tacttttggt atcatgatca tcagtttgat tccttggaca accttgaatg cgaatttcac
     1141 gatttttgaa tcattgacaa actggttaac aaaccttccg gtactaggta cggttctagg
     1201 taaaagcatg cttccattag gtgactggta cttcccagaa attactatgt tattcttagt
     1261 aatgtccatc gtagtagcaa tcacttatgg catgaaagag tctgaatttg tctctgaatt
     1321 tttagctggt gcttctgatt tgatcggtgt agcaatcgta gtagctgttg ctcgtggtat
     1381 ccaagtggtt atgaacaatg gattaatcac agacaccatc ttacattggg gcgaacaagg
     1441 attaagttca ttatcttcaa gtgtattcat cattattaca tttattttct atatccctat
     1501 gtcattcttg attccttcaa cttccggctt agctgctgca acaatgggaa tcatgggacc
     1561 tatgggtgaa tttgccggcg tcggtaagga tttagtaatc actgcttatc aatctgcatc
     1621 tggtttagta aacttaatta caccaacatc tgctatcgta atgggtgctg tagcaattgc
     1681 tcgtttcgat gtttcagtgt ggtggaaatt taccggtaaa ttaattgcga tattatttgt
     1741 agccatctgt gtgatcctag gtgtagcagc aatgttctaa acctagacaa atacaggtac
     1801 aaggtctatc ataagaatga agaaaccgat aaacggctat cgataagcac gtggataagt
     1861 caatagctcc attcagtgag tgttttttct ttcatcattt gttaagaaca ttctgcttgc
     1921 tgatggagca ggcttaaaac gtcattattt gagaagaaga ggttgtgtcg gaggaatttg
     1981 acatgaccct ttttaacata ttaggaggat aatatcatga aaaaatttgt gacagaacaa
     2041 catcatgaac aagcattaga atcattgagc gaattgatcc gaattccttc cgtactagat
     2101 gaagcagatt caggacaagg acatcccttc ggtaaaaaag tgattgaagc tttggataaa
     2161 gtattggaaa tcagtgaaaa gctaggattt agaacattta aagatccaga aggctattac
     2221 ggttattcag agattggctc tggagatgaa ttattcggta tcttgtgtca tatggacgtt
     2281 gttccagcag gggacgaaaa taattgggaa acaaaaccat tcgatccaac tataaaagac
     2341 ggctggttag ttggtcgcgg atcccaagat gacaaaggtc cttctattgc ggcaatgtat
     2401 gccgtcaaag ctttgattga tgctggcgtg gaattcaaga cacgtattcg ttttatcttt
     2461 ggtacagatg aagaaaattt atggcgttgt ttagaaaaat ataacgaaaa agaagaagga
     2521 attacgcaag gatttgcgcc agatgctgaa tttcctttaa tttatgcaga aaaaggctta
     2581 ttgcaagctt acttgacagg accaggaacg aacgagtttt ctgtaaaagc tggaggagcg
     2641 ttgaatgttg ttcctgatgc agctccttac tctggagaaa agctttctga agtaaaagaa
     2701 gcattgaaaa aacacgaatt cgatttcgaa gaacaaggtg aaggcatcgt cgttcttggt
     2761 aaaagtatcc acgcgaaaga tgcagcacaa ggagtgaatg ctatttctcg tctggcaatt
     2821 gctctttcag aagtatttga ttttggccca atcaatttct taggaaaatt agttcaagaa
     2881 aatgcaactg gtgaagctgt cgtgggcaaa acagaagatg aacaatccgg cgagctaacg
     2941 atgaactttg caagtttgga aatcacacca gaacaaacaa aaatcggtgt agatatgaga
     3001 atccctgtta cattcaagaa agatgattta gtagctaaat taactaaaac agcagaaaaa
     3061 tatgggttga cttatgaaga atttgacttc cttgattcat tatacgtgcc actagacagt
     3121 gagttagtga aaaatctatt ggggacatat cgcgatatta ctggagacat gactgaaccg
     3181 tttgtatcag gcggcgctac ttttgctcgt acgatgaacc agtgtgtggc attcggtgcg
     3241 atgttccctg atactcctga ctttatgcac caggcgaatg aacgatggga attaagtagt
     3301 atgtataaag caatggaaat ttatgcagaa gctgtgtatc gtttatgtgc taaataaaaa
     3361 taaacataca aaaagggcgg atattcaaat ggatattccg tcttttttta tttgatgctg
     3421 gaaagtttgc atttctctct tattttttta aggtataatc atacttgatt aagaaaaagg
     3481 ggtcactcag atagaaaagg tgggagaaag tgaaaatatt tagacgaata ctaattggct
     3541 tagtcatcat tttaggatta ctcggtggag ccggttatta ctatatacag caaaatacgt
     3601 acgaaccatc gtcagaagcg caagtgttgg taaaagaagg aaaaaacaaa gaagattact
     3661 tattttttga aggagagaaa aagaatcatg gaaagtctcc attacttatt ttttatcctg
     3721 gtgctctcgt agagccagaa agttatagta tttgggcgca agatttagca aaaaacggct
     3781 tttcagtcgc tattgtcaaa atgccacttg atctagcagt gatgggcgga aacaaagcgg
     3841 ataaggtcaa agaagatttt ccaaattccg attatgtgat cggtggccat tcattaggtg
     3901 gcgtaatggc aagtcgttat gtcaataaga atcattcaga caaacattta aaaggggtat
     3961 tcttcatggc aagttatccc gacaaaaaag gaacattgac agaatcagct gttcctgttt
     4021 tatcgatcac ggcgacaaaa gataaagtat tgaatcagga agcttatcaa gaagctaagc
     4081 agtatttacc ggaaagaaca gagtatgtat caatcaatgg cggaaatcat ggaggttttg
     4141 gcagctatgg tgagcaaaaa ggagatggag aggcagaaat ttctaacgca gaccagcaaa
     4201 ctgtgatcgt caatacattg gaaaactggc tgaaaaagtt gaaataatat aaatgggtcc
     4261 tatagggtcc gtgaaaaaac tagtcaatta actatagata gcaaatgaat ctttcaaaat
     4321 aagtcgggat ttcacggaac aagagaaaca gatcccggct tattttcaag tttagagaac
     4381 ttagtgtacg tttttaaagt ttcgtttata tttatatttt attttctaat aaaatcgctc
     4441 gaactggaca tttttgatac gcttttttta catatgtttc ttcttccggc atcacgaact
     4501 gttgatgagc ctctggttct tgggcaaata aaacgattcc ttcttcatca taatcgaaaa
     4561 tatctggcgc ttcgatttga caaagaccgc atgcaataca gcgttcaggt acaagtttac
     4621 atagtagtga catttctaac catcctaact taaggagaat aatatgattg tttttatttt
     4681 atcgctattt tcatcaggac acaagctgcg gattagttct ctttaccaac tccttgtggg
     4741 aaaacgaacg acttcagtac ttatttatgg atttacccac gaacttttgt ttatccataa
     4801 cagttttcca gaattgaagc aggataagtt ttatcaaatt ttgcaaaaaa ttgcgcagca
     4861 aggttggatc gaaataaatg aaaatgaagc aaagctaacg cctgcagggg ctgatatgtt
     4921 gtccgaacat caaatagaac atactggttt gcgttttgac cgctatggca gaacagggga
     4981 aactagttgg cggctcatta aatttgctgt tcaagtaatt tctaatctgg caacgggtat
     5041 tcaagactat cttccagcag agacaagtcc tttttatact ttccaactaa aaaaatggct
     5101 tagtgaaagc caattgccac gtgaaatttt gattgatact gcgtatgaga gtttggtgca
     5161 gctgttttcg gagataccag aagaagcagc agatttttta gctaaccaat tatctggaaa
     5221 tgagcgaaca gggctgcttc cttatcagct ggcaaaaaac aatgatgagt cagcggttta
     5281 tctgcaacaa agcagatgta tccacctttt gttagcacaa atcgaaaaac ggccagattc
     5341 gctttggcac gctttgatcg attcattatt acagcaaaat ttcaatcaaa gcatgatgat
     5401 caccaagcag atgtttatga atggtcagac aattgaccag atcatggcaa tccgtcattt
     5461 aaaaagagga acagtaacag atcatcttat tgagtgggcc ttgttttttg atgattttcc
     5521 atacgagtgg attctctctg cagaaacaat cgaacgtttg gaaccaaata aggattccgt
     5581 gcgagagtgg cgctttagcg aatggaatgt agatggacaa ttagattatg gagaatttcg
     5641 tttgtatcaa atctatctat tgagaaagga agcgatacag aatgttaata aatgaattaa
     5701 aaaaatattt tggatttgat tcatttcgtc ccggccaaga agaaattatt caggcgttgc
     5761 ttgatggaga agatactctc gctattttgc ctacaggaac gggaaaaagc ctttgctatc
     5821 aattgacggg gtatttaatg gaaggtctgg tcatcatcgt gtcaccgctg ctttctctaa
     5881 tggaagatca agtgacgcaa ttacaaaaac gaggggagaa acgagtagct gctttcaata
     5941 gtttactttc gcaatctgaa agaagatacg tcctccgtca tctatcgcaa tataaatttt
     6001 tgtttttgag tcccgaaatg ttgactaatc cttctctttt agagcatctg aaaaaacaaa
     6061 atattgcgtt atatgtagta gatgaggctc attgcgtgtc tcaatgggga gttgatttcc
     6121 gtccagaata tcaacagtta ggaaaaatcc gaaagctttt aggaaacccg gtcacactgg
     6181 cactaacggc gacagcaaca gatttagtag caagagatat acgtcatgta ctatttgatc
     6241 gaccaccaaa agagatcaga caatcagtca atcggcaaaa tatctcgctt tttgttcgga
     6301 aaacgcagca aaaagaacaa gaacttgaac acttcatgga aagagcacaa ggagcggcaa
     6361 tcatttattg tgcaacaaaa aaagaagtcg aacgattgta tcatttattc agggaacgtt
     6421 ttacagttgg atactatcac ggtggtcttg atgcagctca aagaagacaa cttcaacagc
     6481 agtttgtaaa aaatcagctg cagtttctca ttgctacgaa tgcatttggt atggggatcg
     6541 ataaatctga tatccgttat gtggtccatt atgatttgcc agacagtcta gaaaactatg
     6601 tacaagaaat cggtcgagca ggcagagatc aggaagaaag tgcagcaatt cttttttatc
     6661 aaagtgggga tgaacgtatc cattatttct ttaatcaact ttctagagaa cagcgacaaa
     6721 gttttgagct ttatttggaa tatgccacag agcaagctcc ctttgatgag ctgcaaaaaa
     6781 aatggatgga gctgatccag cagtctgaaa aaccagaaaa ttgggtggaa cggctcaaac
     6841 gtcaggaaaa agaaaaagaa ttccgcctgc agcagatgct tcgctatatt aatgaagaaa
     6901 actgccgcag aaaattcatc ttagcttatt ttggcgaaaa actctccgaa aagccgcaga
     6961 attgttgtga tattgatgga gcaaaagcag aacttctgcc aaagaggaag cttttttcta
     7021 agactgaaca acttcattgg ggaacgattt tactaaattt atttaaaaaa aacatggaac
     7081 attgattttt taggacaaga cactttttcc tatggtataa tacacacgat agagtttagg
     7141 aggtaaaaca gtgagcagaa aagatagaca tcaatctaca gaaaataacg aaacacaaga
     7201 accatgggaa caaccaatct acgatacaga tgatgaagtt tcatcaagaa gttctcagag
     7261 acaacaaaag aaaggaaata cattatttct ttcgctattt ttgattttac tggtcttatg
     7321 tatcgctatt ccagcaggtg cttatttctg gatcagaaat ggatcaagca acaatgcaac
     7381 agcagcaact tcttctgaaa cttcgacatc agtagtagaa agttctacaa gtacaacaaa
     7441 agaggagtca actgagactt caacagtaga atctagtaca atcgcggaag aatctactgt
     7501 gtctcaagat acaggtgtaa cgtcagaaag cagtattgca gcagagacaa caccgacttc
     7561 ctcaagtgag gcaacttatc aagcagggga ttcaagcagc aatgcagcag aaacaacacc
     7621 ttcttcatca gctgcagccg gttcaacaac gactgttcaa gcaggcgaag gtccaaaaca
     7681 agtggctgag cgtgcaggga tcaccactga tcaattgttt gaattgaatg gcttagatcc
     7741 aaacaacttt atgctttacc ctggtcaaga attaagaatt aaataataat agccgcgctt
     7801 tgcggctttt tttctgagaa aaaggagtta tatcatgaca caaatcagta ttgcaatcga
     7861 cgggcctgct tcatctggga aaagtaccgt tgccaagata ttagcaaaag aattgaatta
     7921 tatttataca gataccggtg ccatgtatcg cgcagtaact tatttagcta ttacgaacaa
     7981 tgtatctttt tcagatgaag aagggctagt ttcgctgatc aaaaagtatc ctatctcttt
     8041 ttcgcaacaa gaagatggac agcatgtatt catagcatca aaagatgtga ctttagaaat
     8101 ccgtcaacca gatgtcacaa aagctgtttc tgaagtctct gcacatggaa aagtcagaga
     8161 agaattagtt gctgtccaac gaaaaatagg ggaacgcggt ggcgttgtca tggacggaag
     8221 agacatcggg acagctgtat taccgaatgc agaagtgaaa atctttttag tagcaagcgt
     8281 aagtgagcgt gcagaaagac gtttcaaaga gaaccaagaa aaaggaattc cgactgatct
     8341 tgaaacactg acgaaagaga tacaggaaag agaccattat gattcaacaa gggacgtctc
     8401 tccgctgaag caagcagagg atgctgttcg tatcgatacg actggcaaat cgattccaga
     8461 agtcgttgca gcaataaaag ctgttgtttt gaaaaaaggt tatcttcttt tttaaattaa
     8521 gaaaaaaa
//
LOCUS       DANKHL010000123         8514 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000123 DANKHL010000000
VERSION     DANKHL010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.127"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(22..1722)
                     /locus_tag="PW101_001976"
     CDS             complement(22..1722)
                     /locus_tag="PW101_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514371.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            2229..3389
                     /locus_tag="PW101_001977"
     CDS             2229..3389
                     /locus_tag="PW101_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2514372.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            3405..3848
                     /locus_tag="PW101_001978"
     CDS             3405..3848
                     /locus_tag="PW101_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2514373.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(4331..4861)
                     /locus_tag="PW101_001979"
     CDS             complement(4331..4861)
                     /locus_tag="PW101_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HDL2514374.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            4966..5517
                     /locus_tag="PW101_001980"
     CDS             4966..5517
                     /locus_tag="PW101_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2514375.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(5670..6143)
                     /locus_tag="PW101_001981"
     CDS             complement(5670..6143)
                     /locus_tag="PW101_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2514376.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            6281..7753
                     /locus_tag="PW101_001982"
     CDS             6281..7753
                     /locus_tag="PW101_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2514377.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            7750..8460
                     /locus_tag="PW101_001983"
     CDS             7750..8460
                     /locus_tag="PW101_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HDL2514378.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         2801 a         1495 c         1652 g         2566 t
ORIGIN      
        1 ggatgtattt atcccttttg cctaagcacg taaaagcttg tatctatgat aaataaataa
       61 acatagcaag gtaaaccagc caatcaaagt aagagaaaga atgaaataga aatagattgg
      121 cgtttgataa tgaagcgtga gctcgttgat tcctttttgc tgcttcacga ttggggtccc
      181 gatatctgtt acttcataat cagtcaatgt ttttccattt tgctgtagca cagtccgatc
      241 gtagacgata acagggatat gaacagtttt gtccccagtg tttttccact tgattgtcaa
      301 aagaccgcct ggatgtgttt ttataaaagt tgtttcgttt tctagtattt cttcgttata
      361 ccggtcgtat ttattgtctt ttgtctcctt atagattggc agatagtcag gtgtggtttt
      421 ttggaaaacg aacaagctgg tagacatatc tttgctgaag aatgttttcc ttgcttcttc
      481 cggcgtatcg aatagataag tatgtcgact gacaaatgtt tcattttccc acttttccag
      541 atgctggtat aagttctgga tcgtctgtgc tgaaaaaata agaatgccaa atatacttaa
      601 aagacggaac cacttttgtt cagaccactg gttcagtgaa agggcaaggt aaagtagaag
      661 caatagcgta aatggcacga aaaatcgaaa tggaaactgg atcaaactaa taaaaggcac
      721 atttttttcg atcaatgtcg tccatggaat caagctagta gaaagaaaaa agaaaacaag
      781 acttgtgtac aacacgattt tttggaataa ggtgttcttt tgttgaaaga aaataaaaag
      841 caaaaaacct gataatatca atataggaaa ggccttaggg taatggagcc aataactgcc
      901 atccagatct atcgcctttt gactcatttc tctgttaaca aagggaggaa gaatcttatt
      961 cccactgtaa acaatagcta atcctgccca aatatttgct gtcagaacaa aataaagcaa
     1021 gaccgactta atcagttgaa gcagtgcttt tcctttttca gtctggttaa aaaagtaata
     1081 aagataaaaa gggatataca tcaaagctaa catgatcgaa gacagtaaat ggatctgtgt
     1141 cataagtccc actgagacag cgactttgat aacagggaac tttttatgaa ggacgaaatc
     1201 agcaataggg atcatacata aaggataaaa acaagtcccc cagctagtga atccttggtt
     1261 gatagaccag tattgaatag aatacgtact catgaataaa acggctactg gtacacttac
     1321 ccaatttcga acattgacat atctcagtaa aatcagcata gaaaatcctg aaataagata
     1381 aagaatgaaa tttgaaagaa tctgataatg aaaccagcta cctgcaagca acacgatcaa
     1441 tccttgtaaa taagcgaaaa atgggccgta aacggcattc acgatcctgc cactttgctg
     1501 gaatccataa agggaaggaa aataatggaa atccccatat ttaatttgtt gcgcggcatc
     1561 atagaaccga ttgtaatgga atggggcatc agcacccaaa attagtttat gtgtgaataa
     1621 atgaggcatg agaaatgcca aagcaatgaa aaaaattgct aaaaatgggc catatttttt
     1681 caattgtcga actcctttgc aaactttaga taaattcatc atatatccta tgcgagaggt
     1741 aggcaagaaa ttagaaaatt catgaaaata tttatgaatt ttacaagaaa aacataagtg
     1801 aaagttctaa ggagagagga tgcgacataa gttttgttgc atctccttgt tttgaaacag
     1861 aatttttctt aatactcgaa acaaagtagc tttttcatga cctctcggta aacggtgttc
     1921 aatcagctgc ttctcggtaa taagctcact agcctagaaa atatgagaag ctattttctg
     1981 actagcgctc ttattactca aagcataacg cagttttcac aacctcttcc ttaaacggtg
     2041 ttcaaaagct tgcatctgcg gtaataagcc caacccaaca aaaaatatga gtagctattt
     2101 ttttgggctg tacttattac ttgatgcagg acagcttttt cacaacctca tcggctttca
     2161 ttttctattt ttttcctgaa cccatataat aaaagaaaga acaaaaaaac ttgcaaggga
     2221 ggacacatgt gaagcagagt aagcaaaaac agtctcgtcg acttttgttt cgtttgtttt
     2281 tattaatatg tatcgtcgta gggttactga tcgctttata tccattttat gtggattctt
     2341 taaatagttt tattgatcaa aaacgtatcg aacaagtaca aaaacggacc gcagctgaaa
     2401 ttgaagtgca gcgaaagaaa atggaggaga aaaaccaacg attaactgac caaggattca
     2461 atcctggtac tgatcctttc aatgaaaaga acagaaatga aagtacaaca tccagccaat
     2521 tagaggaatg gctgatcgga agtatcaata tacctaagat ccaattaaat atttcacttt
     2581 atgatcggct caatggtagg atccttgaaa atggagcagg cgtcttgcaa ggaacctctt
     2641 tcccgcttgg tggaaattcg acgcattcag tgatctctgc tcacagcggt ttacctaatc
     2701 gacggttatt taccgaatta gatcgtttag aaaacggaga cacgtttatt ttgactgttt
     2761 tgggggagaa attggcttat caagttaaaa atatacaagt ggtactgcca gatgacacgt
     2821 ctgttctaac gatttcagaa ggagaagact tagttacctt gcttacatgt acgccgtata
     2881 tgatcaacac gcatcgtttg ctagtgacag gacatcggat tccttataac gaatcggtaa
     2941 aaaaagaaga aggaaaagga aatcaggaaa gaaccatgcg gcaatggttt attcttgcag
     3001 gaacgataat agctgtagtc atactgttag tgtttatcgg acgactaatc tatcagtata
     3061 gattaagtaa aaaagtgctt gatttttcat ttattattag tgattcgtca gggaaaccgg
     3121 taaatggtag ttcattcatt ttgaaacata agaaaaaaac acttactaga aatggtgtgc
     3181 cgttttcagt acaaagcgac catcatggga aagtcaagct ggatcaattg cctggtggaa
     3241 cttatcgaat cgtttcagct gatgatccta aagttgctgt ttcttttggt attagaaaag
     3301 taaaacaaga gaagatatac ttttttgagg ggcgaaaact tgtaaaagag cttcaaaaaa
     3361 atggttttta ttttaaaatc aatgaataag taaggagaga aaaaatgatc cgcacaacaa
     3421 caaaattgtc tgccaaagaa ctggaatctg ttctatcgat ttggctgcat gctaacttgg
     3481 attctcatga attcattcca tcaacttatt gggagaatca agtagaatct gttaggaaag
     3541 ctttaccaga tgcagtgatt tacttagcag aaatagatca agaaattatt ggttttgctg
     3601 gggtgattga tcagtttatc gcaggaatct ttgtgaaaag ggaataccgg caacaaagaa
     3661 tcggtacgca actaatgaaa aaaataaaag aaaccaataa aacactgact ttgacagtct
     3721 atcaaaaaaa tcaatcagct attcatttct atctttcaca aggttttaca attcaaacac
     3781 agcaaaaaga tgaagcagta aatgaggagg aacttgtgat gaaatggaat caaaacgctg
     3841 atttttgatg aacagaataa cgaaataatg ctcccagcct aattttttgc tgggagcatt
     3901 atttcatttt gaagtcacta atttaactgt gtgaatatgc agttgttaca atctttgata
     3961 aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac
     4021 tatttttcca tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttgat
     4081 aaacggtgtt caaacggctg cttctcggta ataagcccac tagcctagaa aatatgaaaa
     4141 gctattttct aactagcgtt cttattactc aaagcataac gctgatttca caacctcttg
     4201 ataaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaacatgagg
     4261 aactattttt ccgtttcttt ctctaatact caggtcagaa cgctgatttc acaacctctc
     4321 tttcacaacc ttattttact cgtttataat tttcatcgaa gtattttccg ctacggatat
     4381 catctggcat tccatcgtat ggttcttcgc taataacatc ttcattgtct atacctgcat
     4441 cacccatata agtgatcgta gcaaattcag gaacaagtaa ttttggataa gtttcatatt
     4501 tttcacgagc tgcttccatt tctgcgatat gttcgttttg gaaagtaaca gtgatttctg
     4561 gatgttcttc aacccattta gggaaaaaga tcattccgtg aagtttcttt tcatttggca
     4621 taaagtccaa tgccacgtct gtccctgttg gctctgtcca agtgattttg aatacgccgt
     4681 ccgtcaattt aacgatatca gcttcttggt cttttaccca acggcctttg accattccgc
     4741 catggatacg gtaatcaaca gtatggtcat ttttggcata ccattcatat tcccaaccat
     4801 tgtcatacgt atagataaag tgagttccaa taaaatcttc caattcttta aattctttca
     4861 tgtttaagca tctccttata aattaaaaca tgtaataaac tacatgtttt cgattactca
     4921 tttatgatag aatggattta aagatttgtc aaggagggaa aactgatggc aagaaaaaat
     4981 acacttcgat atattttgtt agggttatta agtaagaaga agatgacagg atatgaattg
     5041 aatcaatcgt tcaaaaatga aataggagaa ttttggcaag caaaacatag tcagatttat
     5101 cctgaacttg caaaaatgga agagcaagga atcattcagc atcaagtaga gatcacaggt
     5161 gaaaaattgg aaaagaaagt ttatgaatta acagaagaag ggaaggaaca attggcagag
     5221 tggattcata cacccaccaa tgaactaccg gttaatcgag acgaatttgt tctgaaattg
     5281 tatttcgtca aagacgtgaa tgatccggca ttagtagaaa ttattcagca gcagcgtgac
     5341 cttcatgaag aaaaacgact tcatctatta gagcgaaaaa aaacgatctt tcctactgaa
     5401 aaagaaaaac atgaaaatta cggacactac ctgattttag cgcatgcgat caatcgggaa
     5461 acagaatatg caaattggct aacagatgta ttagaagaaa gtaaccgaaa aaaataaaaa
     5521 aatatccgaa cagtgatgga gaattccttt ctatatgaaa gaaacgcaga tcaccgttcg
     5581 gatattctta tgttcaaaca ggcctttttt caagtattta tgtagcatct acttattaaa
     5641 aagcttggta cttaggagat tagtggcgtt tagagttctt ttaacaactg ttcgtagtca
     5701 gctttttcat cgacaggaag gagcttgtaa gttaattcgt ctgtctcgtt gtcatgaatg
     5761 atgtacatct tagcattctc ttctttgaag ttaccgattt gtaaaatcgt atagcgttcg
     5821 tcgatcaatt cgatatcttt gattgctttt gaaaaaactt tacggacatg gtgcattttc
     5881 ttttggcgga tttctgcctt ttctgcagct tttgacaaag taaatccgtc atctaaatat
     5941 tttttgatca gttctacttt tatgactgta ggtaaacgat attttctagg acaattcgca
     6001 tcagcttcga tcgaacgaat gaatcccttt tgttcccaat accttaattg acgtggagac
     6061 acacctacga tatcacttaa ttcactgatg ccaacaacca attgatcatt ttccaacaaa
     6121 cgtttaaggg aagccccaac catttttgtc actccttttg tcctcttgct tcccttaatt
     6181 atatccgtat taacttatgt gtcaagtagg ttgacaaata gcgaatttag gactaaagta
     6241 ttctagtagt ttcagaaaaa attcagaaag aaggagaaat atgagtaaca accaacccgt
     6301 agatatttac ggaaaaccat ataatcggac acttttagtt gttgtcttat taattggtac
     6361 attttgtacc gttttgaatc agaccttatt gacgacagct tttcctacat tgatgaaagc
     6421 tttcgatatt tcagcgtctg atgtgcaatg gttaacgact ggtttcttgc tagtcaacgg
     6481 gatcatgatc ccaataacag catggctgat caataaattc agttcaagaa atttatatct
     6541 ttctgccatg acgatctttt tgatcggaac gattacttgc tttacagcac ctaattttag
     6601 tacattacta atcggtcgtt tgatccaagc ttgcggtgtg ggagtatcga tgccattatt
     6661 gcaaaatatc atgctatcta ttttcccgcc agaaaaaaga ggatcagcaa tggggatgtc
     6721 agggatcgtt attggacttg ctcctgcttt aggccctaca ttatctggtt atatcattga
     6781 taactaccat tggcgtgact tgtttggtat ggtactgcca atcgttgttc ttgtcttggt
     6841 tttagcattc ttcctaatga aaagtgttat tccattgtca aatcctagca ttgatatttt
     6901 atccgctatt ttatcaacga ttggattcgg tagtttactt tacggatttt ctagtgtagg
     6961 agatgacggc tggggaagct tgaaagtaat tggtttctta gttgttggtg tgattttcat
     7021 cgccttgttc tcatggcgtc aattacattt ggaacatcca ttcttggaac ttcgtgtatt
     7081 caaatcatct acattcacga ttgcagctgt tttagctggt gtaacaaata tggcgatggt
     7141 aggggcagaa atggtcgtac cattgtatat tcaaaatatt cgtggagaat cagcattcca
     7201 ttctggcttg atgttgttgc cgggtgcatt gatcatgggg gctatgatgc ctgtgacggg
     7261 tgcgattttc gataaatacg gcgcgaaacg tttagccatc acagggatgt tcattttgac
     7321 agcggcaact gcaccatttg ctttcttgac aaaaacgact ccagttttat atatcgtgat
     7381 cctttatgcg atccgtatgt ttgggatctc catggtcatg atgccggtaa cgacttctgg
     7441 tatgaatgca ttgccaaata acttgatcag tcatgggaca gcggtaaata atacattccg
     7501 tcaaatcgca agctcgattg gtacagctat tttgatcagt gtgttgacga atgtgacaaa
     7561 agcaaatatc ccaagcaaat ctgtattgga taatacgcca ttagcttata aagatcaggc
     7621 aatcaatgcc acactgtcag gttaccatgc cgcgtttatc gtagcgatca tctttgggat
     7681 tcttggcttt attattgcct ttttattgaa gaaaaaagaa cctgttctaa tgaaagcggg
     7741 ggataatgca tgattattgg agttttgatt gtcagcttgc ttgcttttgc tttaacgaac
     7801 gtgttcgcta aaaaaacatg gcaaaccttt ttatctttga ttttcggact aatcttcgtc
     7861 gcttcactaa gtttgatcgt cgctaatttg tccaatcact ttggaatgga aaaagtaacg
     7921 gaaacaaaaa cagaaaaaat tgtttccagt gctgattccc aaggagcaga tatgcttttg
     7981 tataaagctt tagggaatgg aaaagaaaaa gtgtatcttt accgaacaaa tgaaaaacaa
     8041 gaaaaaccaa aagcaactgg tacggataac gaaacaaata aagtagaaaa gacagacggc
     8101 gatgctgaaa aagtgacgaa aactacttat tgggaataca aaaacgacat gtataaattc
     8161 tggttcaata ttgctgataa caatcacgaa tatgacagcc gagtgaatac attcaaaatc
     8221 ccagagacat gggtagaatt aagtacggac caagcagcca agttagctga attagtgaag
     8281 aaacagcaaa gtacgatgga aagtgaagca aaagcttatg ttcaagacgg tatggtaaaa
     8341 gcaatgactg aaaatccaaa aatgagcaaa gcagagcaag aacaacgtac gaaagcctta
     8401 gctgctgaat tccaacaaca agcattcgca aaattagtga aagaagcaaa aggcgaataa
     8461 tattttgaac accatgtagg aggttgtgaa aaagctgctc tgcatcaagc agta
//
LOCUS       DANKHL010000124         8493 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000124 DANKHL010000000
VERSION     DANKHL010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.128"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..173)
                     /locus_tag="PW101_001984"
     CDS             complement(<1..173)
                     /locus_tag="PW101_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2514379.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="PW101_001985"
     CDS             complement(593..1714)
                     /locus_tag="PW101_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HDL2514380.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="PW101_001986"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="PW101_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HDL2514381.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="PW101_001987"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="PW101_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HDL2514382.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="PW101_001988"
     CDS             complement(4304..5215)
                     /locus_tag="PW101_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2514383.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="PW101_001989"
     CDS             complement(5232..6239)
                     /locus_tag="PW101_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDL2514384.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSIPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="PW101_001990"
     CDS             complement(6236..8365)
                     /locus_tag="PW101_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2514385.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8493)
                     /locus_tag="PW101_001991"
     CDS             complement(8370..>8493)
                     /locus_tag="PW101_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2514386.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1727 c         1486 g         2680 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggaattga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttt
//
LOCUS       DANKHL010000125         8423 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000125 DANKHL010000000
VERSION     DANKHL010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.129"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(57..1010)
                     /gene="menA"
                     /locus_tag="PW101_001992"
     CDS             complement(57..1010)
                     /gene="menA"
                     /locus_tag="PW101_001992"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL2514387.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1023..2126)
                     /locus_tag="PW101_001993"
     CDS             complement(1023..2126)
                     /locus_tag="PW101_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL2514388.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2216..3172)
                     /locus_tag="PW101_001994"
     CDS             complement(2216..3172)
                     /locus_tag="PW101_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDL2514389.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3453..5387
                     /locus_tag="PW101_001995"
     CDS             3453..5387
                     /locus_tag="PW101_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2514390.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5844..6260
                     /locus_tag="PW101_001996"
     CDS             5844..6260
                     /locus_tag="PW101_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL2514391.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6274..6840
                     /locus_tag="PW101_001997"
     CDS             6274..6840
                     /locus_tag="PW101_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDL2514392.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6863..7849
                     /locus_tag="PW101_001998"
     CDS             6863..7849
                     /locus_tag="PW101_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL2514393.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8004..>8423)
                     /gene="scm"
                     /locus_tag="PW101_001999"
     CDS             complement(8004..>8423)
                     /gene="scm"
                     /locus_tag="PW101_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL2514394.1"
                     /translation="STDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2479 a         1624 c         1708 g         2612 t
ORIGIN      
        1 tttggctgtt cttattactt gatgcagggc agttttttca caacctcttc atgctgttaa
       61 atggcttgca taatgattgt tagtaatagc cccaatacat aactggcatt gaataagacc
      121 atatttttga tggaatagct gaaactgata ggctggggta acgtttgctt gaattgatcc
      181 aaatgcttct tcacaattgg caaggtcaag aaggtgacca agatcggcca ttcatagata
      241 ccgctcaaca accccattaa aatcacgaaa tagcaagcat acatcaatag ctggaataag
      301 actgttcccg caggacgtcc gatataaaaa actagtgtat agcgatgatt ttcaatatca
      361 cggtcaagat cccgcaaatt attagccaac atgatattgg caatcgtaaa caccaagggt
      421 aaggaagcta atacgattgc tgctatagcc caaagatttc ctaccaaagc aaattgtcca
      481 gatgaaaagt tgactgtcag atcatagaca tctgaaccga atgtattgac ataaacgacc
      541 atggcaaaaa ttccaaggcc catagtcacc ccactgaaaa tttcccctaa aggcattcga
      601 gataaaggaa tcggtccgaa tgtatagaat actccaataa aacaacagat aaatcccatc
      661 aaaagtaaaa gccaacccgt attgatcgtt agaataacgc cgataacaaa aacaaatgca
      721 atcatagcta aaatcatgtt tcgaatcaat acaggagata gatttctgcg gccaatgaca
      781 ttttcttcat acttatatac ttcagaatga gccttttcat aatccatgta attattgata
      841 gcggtagtcg ctaaatcaaa aactagcatt ccaataaaaa ataaaaaagt gtttagccaa
      901 tgtatctcat gaaaaaaagc gatagagaat aataccccta ccgcaaaagg aaatacacta
      961 gccacctttg ttcgtatttc aactacttct aaaaagattt ttagtgtcat ggttcgtact
     1021 ccttatttta gttcagaacg gtcacccatc gtgtaatggg agtcctccga taatttgaag
     1081 gtgtctttga ttggatcggt cacatatact ttatcgtctt ttgtcacgaa gatcgcttcc
     1141 gtcccgttat tcaattcgct ttcgacgtat tccagtcctt ttttcacacc cattgaaaag
     1201 actgctgtag acaacccgtc accatcaatt gatttatctg ttatgatcgt gacacttgcg
     1261 atatcattgt cgaacggata acccgttttt ggatcaaaca aatgatggta ttttttgcca
     1321 tctacttcta aataacgctc gtaaattcca gaggtaacta atgttttgtt ccgttcttta
     1381 atagaaccta aaacagatcc gcgagccatg ttcggatctt ggatgccgac tgtccagtct
     1441 tggttttcgc ctcttgggct gtggcctaag acatagacat taccgcccaa gtcaacgatg
     1501 gcagtggtca ctccttgttt tttcaagact ttcactactt cgtccgtaat gtaacctttg
     1561 gcgattgcac ctaaatcgat gatcattcct ttttctttta gataaacagt tttttctttg
     1621 tcatttagtt cgatcttatg ataatccact aatttcaatg cctgatcgat ttcttcttgg
     1681 gaaggttttc tggcgtcatc aaaaccgatt cgccataatt gtgtgattgc accaatcgcc
     1741 atatcgaatc cgccttgaga atcttggcta tattcgtagg cccgcttgac taacgtgtag
     1801 acgtcatcag aaacttttac tggtttgatc ccagcttgct cattcacttc atcgatctca
     1861 gaaccttttt gattgatcgt gattttatca ccaagttctt ttactcgatc gaaggcaggt
     1921 ttcaatgctg attctttgcc ttcatcatac acacggatac gaacgtaagt ccctaacaag
     1981 aactgttcat cagaatatgg atctttcaat atcttggtag ctgctgaact ttcagtggtt
     2041 gttttatttg ctgagttgct gcagccagtt agcaccataa aagagatgcc cagtgtgatt
     2101 aaggaaaata gtagtttttt ccgcatcttt ttcctctctt cattttgaaa ggtgagacga
     2161 ctgcctaatc tggctatcgt cccaccctcc aacttttatt agtatgatta tgtgattatt
     2221 tttctgttac gatattatct acttcgattg tttgagtgtc gcctttttca gctgcattca
     2281 ctaattgttg tgcgtacatg atgaatgaat gtgagctgtg tgtagcacct gtaacaactt
     2341 ctacattagc aggtgagcca tcttcttcgc ccattgcttt gacgaattcg tcgttcagag
     2401 tagggatgta agtttttggt tctgtaccgc tttttgcttt catattttca ttatattctg
     2461 tatcatcttg ttttgattta ccgtctttgt cgatataatc aaagtttgat tcagttactt
     2521 tgccgccagc aactgtcatt tcgaattgca cacggtagcc gttagagtag ttcttttctt
     2581 caagcttgta agtgccgtct ttcaaatcag caccattgtc gatttcgatt gtatctgtgt
     2641 taccagcttg agcagcttgg atcaattgtt gtgcgtagtt ttggaagctt tcagatgagt
     2701 gagtagctcc agttacgact tccacaccgc ttgcgctttg tgcttttaag aattggtcat
     2761 tcaactcagg aatgtatttt tctgggtttg tgccagattt tgcttccatg tttttgttgt
     2821 attctgcatc ttttgttttt gattcgccgt tttcattgac gttatcatat tttgattcag
     2881 tgattttgcc gtcttttaca accatttcaa agactgcacg gtaaccattt gaataatttt
     2941 tttcttctaa tttatatgtg ccgtcttgca aatctccgcc agctacaact tttgcagatg
     3001 attctgttgt gctttttgca gtcgaagatt gtgctgtaga tgattcagaa gaagagtttg
     3061 agcttccgtt gtcatctgcg ccgcaagcgc ctaacactag agctgaaaat gctaatacgg
     3121 caaatccagt tacaaaacgt tttttcttca ttttaatacc ccaccattcc atttctagtc
     3181 cacaattttt ctttgtgatt cattatacat tctaatcttt ttagcctata agtcaactat
     3241 ttttgataaa taacccatgt aatatttcaa acgttttctt tcagcaaatt tcgtgaaaaa
     3301 acgctttatt ttaaaatttg aaaaaaatcc aaaaggagtt agacaaaaga acagaacatc
     3361 tatataatat atggagattg tgtaataagt atcaaactaa aagaattatc atatattcat
     3421 tatatagata gtctataagg agcggatgat caatggcaaa gcaaaatatt gtcgttgtag
     3481 gagcaggata tgccggtgtt tcagctacaa aatacttggc aaagaaactg aaaaaagaag
     3541 atgtagcaat tactctgatc gatcgtcatt cgtaccatac tatgatgacc gagttgcatg
     3601 aagtagctgg cggacgtgta gaaccagaag caatccaata cgatctgcag catttgtttg
     3661 ctcgtcaaaa gaatgtacaa cttgtcacag acactgtaat cggtatcgat aaagaacgaa
     3721 aaatcgtcaa gaccaaatta ggttcctatc cttttgatca gctgatcctt ggaatgggcg
     3781 gagagccgaa tgactttggt acacctggtg tcaaagcaca cggattcacc ctttggtcat
     3841 ttgaagattc tgtaaggatt cgggaacata ttgagaagac agtagcaaaa gctgcattgg
     3901 aaccagatga tgaactgcgt aaagccatgc tgactttcgt tgtctgtggt tctggattta
     3961 ccggtattga aatgattggg gaactgatcg actggaaaaa tcgcctagcc aaagattata
     4021 aattagatcc aaacgaaatc acactgatgg tcgtagaggc aatgccgacg atcttgaata
     4081 tgttggatcg aaatgatgct gcaaaagctg aacgctattt gcgtaagcac aacgtggaat
     4141 tattattgaa tgcaccaatc gtagaagtgg ctgctgatca tatcaaattg aaagacggac
     4201 gcaacattcc gactcacaca ctgatttgga cagctggtgt caaaggaact tctgacgctg
     4261 ctgactttgg attagaatca gcacgcggac agcggttgat cgccaatgaa tatatgcagg
     4321 caaaaggcta cgaagataaa aacatctata tcattggtga tttagtcttt tatgaagaat
     4381 tcccaggaac accgacaccg cagatcgttc aagctgctga acaaacagga cacacagccg
     4441 cagcgaatat cgtggcttcg atcaaaggcg gagaaaagca taaatttaaa ggaaactatc
     4501 aaggatttat ggtttctatt ggtgccaaat ggggggtagc taacctattt gataagatcc
     4561 atttaagcgg atttttagct atcgtaatga aacatatcgt gaatttgaaa tacttctttg
     4621 atattcgttc tggttattat atgttccaat atttaatgca tgagtttttc catatcaaag
     4681 atgatcgcaa cgtgactcga ggacattctt ctcgttacgg caatgtctta tggagtgtgc
     4741 cacttcgtat tttctatggt ttagtttggt tcgttgaagc ttccaaaaaa atcattggga
     4801 atggtgaagt gttcaaacca agtacttggt ttggtgaagg atcatggttc actggaaaca
     4861 gtgtgttccc ttggacatgg gaatacaatg taacagatgt tgccagtggt gcttctgctg
     4921 ctggtgatgc aaccacagct gccagcggag caggggcaag tgccggagaa gcagcgactc
     4981 aagctgcaca ttttggtttg agttatgcat acggtgaaga accaatggct gtgattgaga
     5041 agtcaccaga ctggttcaca aagatcatgc aagtgatcat gccaaacaat gatgtagcaa
     5101 tgttcttcca aaaattcatg gtattctttg aaattgcctt ggcattagca ttgattgctg
     5161 gattattcac atggctatgc agtgcgacaa cgattgtatt ggtgatcacg ttctgcttat
     5221 caggcatgtt ttactgggta aacatttggt tcatttttgt tgctttcgca ttgatgaacg
     5281 gttccggacg tgcgattggg ttggatcgct gggtaattcc atggctacaa cgtacactag
     5341 gtaattggtg gtacggtaga ccaagttccc agtacggcaa gaaataaata gataaagaac
     5401 gttatttgaa tgcaagattt caatagaaag gggctgagac aaaccgtcac agcctccttt
     5461 ttatgtaaaa ttaaaggaat aagatgcccg agataagcct ccatgagcaa gacacaggtg
     5521 tcacaacctc tgttacaacc tcttgttata aacggtgttc cagaagtagc ttcttcgaaa
     5581 aataagccga aatttcacaa aaacttgaaa agtaattttc gtaaattcct tcttatttct
     5641 tgaagttgcc cacttctgtc acaacctctt gttataaacg gtgttccacc tgctgcttct
     5701 cggtaataag cccaaagaat tagaaaacat ggaaagctgt tttctattct tcgttcttat
     5761 tactcaaagc aagacacagg tgtcacaacc tcttgttttt atgctaaaat tgggtgagtt
     5821 aatctggaaa aaggagaaca aaaatgagcc ttaaagaatt tgctgaaaaa agtctgctca
     5881 aaccttggga tgtttttatt attttgcttt tgactgttct ttctttttta ccagtcatga
     5941 tttttggtct tcagcaaaac accgcaacag cagaaaaaga agcggtactg cgtgtggatg
     6001 gcacagaaat caaaacattc cctttgatgg ctggagaaga gagcaacaca tatacttaca
     6061 aagatgaaca cggagattat aacttgatcg agatagatgg cgatcgagta cggataaaag
     6121 aagcagattg cgatgaccag atctgcgtac gtcgaggctg ggcaacgaaa aatggcgaaa
     6181 ccatcgtctg tttgccacat aaattagtga tcgaagtccg ttcaacagat ggaggtgacg
     6241 aagacagtct gatttattag acctttacac accatgagta aattacaaaa aatgatttat
     6301 atttcgctac ttgttgcaca aggagtgatc attggtttat tggaaaatat gatcccttat
     6361 cctttttctt ttgcgcctgg tgcaaaacta ggcttagcca atctaatcac gatcgttgcc
     6421 atttttacca tgaaaaaaca agacagcttc ttactacttt ggctacgact attcctgact
     6481 actttgctag gcggaacgat ttccacattc ctttatagta tgagtggtgc tttgcttagt
     6541 tattttggaa tgttattggt caagcaatta ggaccaaaac gtgtcagtat catcggaatt
     6601 agtgcggccg gcggtttcat gcataatgtc ggacagcttt tgatggcttc tttcattgca
     6661 caatcatgga cagtcatgtt gtacttgcca gtcctttcag ttttagggat tttatcaggg
     6721 attgcaatcg ggatcgcagc gaattatctt ttacgacatg tgcagacact tcaacgattc
     6781 caaaaagatt atgagcaggg aaccaaacaa aaatggagcc caatgtttac caaaaaataa
     6841 tcgtcacaca aggagttttt ttatgaagct acatccaatg tgggaaaact atcctgcttt
     6901 aagtaaagaa ttgacagaaa cactaaaacg gatggaagaa actgtccgcc tgaaaaacaa
     6961 acctgtcgaa caagcaatca aagaaacgat ccatgccggc ggaaagctgt tgcgtcctgc
     7021 ctatcagcta ttgttctcac aatttggacc agagcaagat cgagacaaag ccatcgcttt
     7081 ggcagcttct atcgaaatgc tccatacggc cacactgatc catgatgata tcgttgatga
     7141 agcggatctg cgacgtggtc agcctaatat ccgtagtcag tttggtaatg tcgtttctgt
     7201 ctatgcagga gactatttat ttgtctgctg ttttaaacta ttgtctgatt actccacttc
     7261 cttgaaaagt ctccaattga attctcgcag tatggaaaaa gtattgaatg gcgaattagg
     7321 gcagatggat gatcgctata attatgaact ttctgtaaaa gaatatttgg aaaatatttc
     7381 aggtaagaca gctgaactgt tccaattaag ttgttctgtt ggcgcttttg aaagtggaac
     7441 aagcgagcgc ttcgcaaaaa aagcaggaga tatcggactt tctatcggga tggcctttca
     7501 gattatcgat gatattttag attatacaaa agagagccag gaaatcggca aacctgtact
     7561 agaagacatg cgtcaagggg tctattcttt accgttgatc tactctttgc aaaaaaacaa
     7621 accaaagcta ctaccttatt tagagaagaa agcggcaatg acagaagaag atgtcgacgc
     7681 cgtacgcaaa atcgttgaac atacaggagg cgtggaagaa gctaggaagt tagcggcctc
     7741 ttacacagaa aaggctttga aggaaataaa gaaattacct gccacctctc ttcgaacaaa
     7801 agagaatctc ttttccctta cccaactgat tttggatcgc aaagactgaa tagggaaagg
     7861 ttcgatacgt aaataaattt ctcagaagga aatctacgtg ttttttgaca aattgaattg
     7921 tagttcttgt atttatgata aaaagcaaaa ataaagagca acgtccgtta gcgacgttgc
     7981 tcttttttat cttgaatatt tttttacctt ttcgatttct tataactcat aaaagtgatt
     8041 cttaaaccga ttacaagtaa tataacgcct gcgagtgtta tccaattatt cgattgactt
     8101 ccagttttag gaagtgctct ttttgatttg tttccattgt cattgcttgt tttattagtt
     8161 aagttaaagt tcgtcctgtt atccgaactt gtttttgaga aggagacact tgcatgacgt
     8221 gtatcgcttg tggtagacga cgtgtctgta ctatcgcttg aagttgatga atctgagcta
     8281 tcacttgtgg tagaggatgt atctgtactg tcacttgaag ctgacgtgtc cgtgctatca
     8341 cttgtagtgg tagatgaatc tgagctatca cttgtggtag aggtagtatc tgtactgtca
     8401 cttgaagctg acgtgtccgt gct
//
LOCUS       DANKHL010000126         8417 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000126 DANKHL010000000
VERSION     DANKHL010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.130"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            9..80
                     /locus_tag="PW101_002000"
     tRNA            9..80
                     /locus_tag="PW101_002000"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Glu,seq:ttc)
     regulatory      269..348
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            346..1539
                     /locus_tag="PW101_002001"
     CDS             346..1539
                     /locus_tag="PW101_002001"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDL2514395.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1669..3141
                     /locus_tag="PW101_002002"
     CDS             1669..3141
                     /locus_tag="PW101_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2514396.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3224..4012
                     /locus_tag="PW101_002003"
     CDS             3224..4012
                     /locus_tag="PW101_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDL2514397.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4079..4747)
                     /locus_tag="PW101_002004"
     CDS             complement(4079..4747)
                     /locus_tag="PW101_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HDL2514398.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4862..6574
                     /locus_tag="PW101_002005"
     CDS             4862..6574
                     /locus_tag="PW101_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2514399.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6577..8349
                     /locus_tag="PW101_002006"
     CDS             6577..8349
                     /locus_tag="PW101_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2514400.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2567 a         1413 c         1851 g         2586 t
ORIGIN      
        1 attttattgg cccgttggtc aagcggttaa gacaccgccc tttcacggcg gtaacacggg
       61 ttcgaatccc gtacgggtca tggaagatac tttttgagta tctttttctt tttatctgaa
      121 agaatgattt taaaaagctt agcagagtta tctttatgga tgattgtgct aagctttttt
      181 ggacagttct aatgtaaaat atttttatat tttttaagca aaaaaatgaa aaaatacgag
      241 cagaaaactg ttttatctgc tatacttggt tcgttgcaag ttcccgaaag gatccgcagg
      301 acggagatgc cttgtaaccg aatttaacta gggggaatca ttataatggt agaaagacac
      361 ttatttacat cagaatctgt ttctgaagga catccagata aaattgctga ccaaatcagt
      421 gatgcaatct tggatgcaat tttaaaacaa gatccaacag cacgagtagc gtgtgaaacg
      481 tctgtaacaa ctggtcttgt tttagtattt ggagaaatat ctacgacggc atatgtagat
      541 attcaaaaag tcgtacggga aacaataaaa gaaattggtt atacacgtgc aaaattcgga
      601 tttgatggag atacagcagc tgtattggtt gcgattgatg aacaatctcc tgatattgct
      661 caaggagttg atgaagcact tgagatccgg gatgaagata aaaaagacgt attagatgag
      721 attggtgctg gtgaccaagg tttaatgttt gggtttgctg tagatgaaac accagaattg
      781 atgcctttac caatcgcttt gagtcatcgt ctggtacgac gcttggcaga tttgcgtaaa
      841 tcaaatgaat taacgtattt acgtccagat gcaaaatctc aagtaacggt tgaatatgat
      901 gatcaaggac aaccggaacg cgtagataca atcgttattt caacacagca tgatgatgca
      961 gtagataatg aaacaattcg tcatgatgtc atcgaaaaag tagtgaagga agttattcca
     1021 gctgaattat tagatgatca aacgaaatat tatatcaatc cgactggccg atttgtcatt
     1081 ggtggtcctc aaggggatgc cggattaaca ggaagaaaaa tcattgttga tacgtatggc
     1141 ggttatgctc gtcatggtgg cggtgctttt tctggtaaag atgcgacaaa agttgaccgt
     1201 tctgccagct atgctgcacg ttatattgct aaaaacattg ttgcagcagg gcttgctcgc
     1261 aaagcagaag tacagctagc ttatgcgatt ggtgttgctc aacctgtttc gatctcaatc
     1321 aatactttcg gtactggaac ggttccggaa gaagaactga ttgctgcagt gagagaaaac
     1381 ttcgatcttc ggccagcagg aatcatcgag atgcttgatt tgcgtcgtcc gatttacaaa
     1441 caaacagctg cttacggtca ttttggtcgt acagatgtag atttgccttg ggaacatacg
     1501 gacaaagtag atgcgttgaa agcaagttta gcaaaataag ttatattcag ctggaacgat
     1561 cgccaagtgg tggttgttcc agcttttttt atggcagtta acctcttgaa aaagatagta
     1621 attatttatc tatttttaga tcctgtcata atgaaaggga ggaataagat gaaaaaagaa
     1681 acaaatgtta ctttggtcac agtgagtatt ttcattgcaa cgttcatgac ggctattgaa
     1741 ggtacaatcg taacaaccgc tatgccaacg attgtcggtt cattacatgg aatggaaatc
     1801 atgaactggg tattttcgat ttatttgctg acaaacgcta tgcttactcc gatttacgga
     1861 aaacttgcgg ataaagtggg acggaaacca atctttattt ttgggacatt attgtttatt
     1921 atcggttcgt tattttgcgg tttatctcaa agcatgattg ggttgattgc tgctcgtgcg
     1981 attcaaggga tgggtgctgg ggcaatgatg ccagtagctt tgacgatcat cggcgatctt
     2041 tattctattg aaaagcgagc aaaagtttta ggtttgaata gttcagcctg ggggattgcc
     2101 agtgtttttg gaccgcttgc gggaggattc atcgttgaaa cgatcagctg gcactggatc
     2161 ttttttatca atgtgccgat cggaattcta ttaattattt tgatttggct atatttaaat
     2221 gaaccaaaaa tcatacatga gtctaaacca atggatattt ttggtagttt tagtttgatg
     2281 gccatgcttg tctcattgct gttagggttc caattattag gagacgaagg aatgagtccg
     2341 cttgtttctg ctttgctgct aggtagtatc gtaatgttag gtatttttgt ttttgccgaa
     2401 aaaagagcta aagatcctgt catcatgatg catttattca aaaatccact atttgttgtg
     2461 gtcaacttgg ctgcagcatt ggctagtggt tttttgatgg gaatcgatgt ttatatccct
     2521 atgtggatgc aaggtgtatt agggctgaat gcagcaattg gtgggctggt attagcacca
     2581 ttatcactag tctggatggt cggttctttc cttgcaagca agtggatgac tcaaatgaaa
     2641 actcattggg tcttgagaat tggactacta atcgcagcta ttgggggcgc ttggttgtat
     2701 cttatgcctt tcagaagtaa ctatttatgg ttttttgcta tatcaacggt catcggtata
     2761 ggcttaggtg tgaccatgac tacctcgact gtcagtgcac aaaatagtgt atctcgtgac
     2821 cagcttggtg tagcaacttc ttttaatacg ttagttcgaa caattggtca aacagtcatg
     2881 gtttctattt ttggtttatt gataaatggg gtaactaaac gagaacttgc agcagcaaat
     2941 ttaacaaatg atgcagatat catgaataaa ctggtgaatc cacaaacggc taaatatatt
     3001 gatgcggata ttttgttacc attaagaaaa attctttatg atgggttaca ttccgtttat
     3061 ctagttggct tgttactcgt gatcattgct ttggtcttaa cgcttttcgt tcatgaaaaa
     3121 agtggtaaaa taaaacagta gtttcggtta agatatgtac gtagccgaat cattcaaatt
     3181 taaagttatt caaaaataac ttcttactaa ggaaggcgaa aatatggcat ttattcaagc
     3241 aaatatttat tcgaatgtgt tagaaatgga agtcaatcta aatgtgatac taccgcaaac
     3301 aacaaaaaag gtgattggca ccagttcaga gcaagagaca acggatatac ctgttttata
     3361 tttacttcat ggaatgggtg gcaatcatag tgtatgggaa agacgtacgt cgattgaacg
     3421 ttatgtagca aactacggat tagctgtcat catgccatct actgatcttg gctggtacac
     3481 agatacagtt tatgacatga agtattggac attcattgca gaagagctac ctgagatttg
     3541 tcatgaatta ttcccgcaat taacaagaaa aagagaaaaa acatatgccg ctggattatc
     3601 gatgggcgga tatggcgcgt taaaactagg ccttgcaaaa tcagaaaatt tcgcagcagt
     3661 aatctctctt tcaggcgctg tttctgtagg aaatcgaatg gatgatcttt tgatggttcg
     3721 taaagcatct ttttgggaag ggatctttgg accattggat caaatcgaag gttcaaaaaa
     3781 tgatccgcaa tacttattga aagaactagt aaaaagcgga aaaccagttc caagatttta
     3841 tatggcttgt ggggaatctg attttcttta tcaggcaaat caggaaatgg tatctgctat
     3901 gcaggaacaa ggcttggcgg tcacctttga acaaggacct ggcgatcata actggatctt
     3961 ctgggaccaa tggattcaac gtgcattggc gtggctaatg gaagaaaaat aaaaaaagag
     4021 tcttaaaaag atagctccta tagagatcac aaacatagtg atctctatag gagctttttt
     4081 actgctcgtt ttctcttctt ttattttgtt ggcataagcc ttcaatatag gtttgtgcac
     4141 tcgttagaaa gagtgctttt tcattcaatt ctgcatggga ctgcatactt tctttaaatc
     4201 cctctaattg attttcaact acataattac gcataaatag aactgtttct ttcaaaaatt
     4261 catttccgtc cataattttt tgtcgaagct gctgttcatg ctgctgatcc attaactgtc
     4321 caatcaataa attgtggaga gaggcaatgg cctgatgact ttgggtgact gaaaatcctt
     4381 cacgaacaag taattggatc atttgctcca aatgttctaa tcgactcgga aaagcgggca
     4441 cagtattgat ttctaattct gccccacaag ggaaacgtgt ataaaggtca tagtaatttt
     4501 ccatatactg cagcaactgt tcattccaag gcaaagttgg atctggaagc gctaattctt
     4561 cttccattgt ttcagccatt ttttgcaaga gctgttgttt gtttttgaaa taccaatata
     4621 aagcaggtgc ttgaacatga attctgtttg cgatgtttcg catggaaagc tgcgaaagag
     4681 cttgtgtttc agccaggata tcaaaggcgg cctgaatgat gattgcttga gaaagcttta
     4741 tttccatttt atcaacctcg tgtattgcat tatcttttta aacatggtaa attgttagtc
     4801 ggtctaattt tacagtgtta aattagtcct gtcaatttaa acaaaggggt gtaatcaacg
     4861 aatgaaatta atcttacgac atgcgaaaaa atataaagta gctgttttta tttccttgct
     4921 gtccgtagca gtcatggttg cagcagcatt atggcaacct aagttactac agcaagtatt
     4981 ggaagctatt attacagaag ataatgatga aatgaaaaca attggtattt acctgatcag
     5041 tctggctttg ctaggattag ccgcaggtgt aacgaacacg attttctctg caaaagtagc
     5101 tcaaggcgta agtgcagata tccgagaaga agcatttcga aagatccaga cattttcatt
     5161 tggaaatatc gaacagttct ctgctggaaa tcttgttgtt cgtctaacaa atgatatcac
     5221 gcaaatccaa aatttagtga tgatttcttt gcaatcactt tttcgaattc cattcttatt
     5281 tattggtgcc ttcattttag ctatgatcac gatgccgcaa ttatggtgga tcatcgtagc
     5341 gcttatcgtt acggtatttc tgattacggc actttcattt acaaggatgg gcaagcactt
     5401 catgatcatt caaaaactga ttgatcgtgt gaatagcatt gcaaaagaaa acttactagg
     5461 gattcgtgta gtgaaatctt ttgtacaaga agaaaaccaa ttgaagtcat tcagcaaagt
     5521 aagtgaggac ttaacaaaac acaacatcat agtcggtaca ttattttctg tgatgatccc
     5581 gtccttcatg ctggctgcca atttggctgt ggttggggcg atcttttttg tgagcgatct
     5641 tgttaaagat gatcctacgt taattggtgg aattgcttca ttcatgaact acttgatgca
     5701 gattatgatg gcaatcatta ttggcggaat gatgatgatg atgacttcaa gagcagccgt
     5761 atcgctgaag cggattgcgg aaattttaga tgctgaacca gatcttactt atttagacgt
     5821 tccagaacaa gagttgacgg gttctgtcac ttttgaccat gtttcttttc gttatcctgg
     5881 tgaggataca gatacgctta aagatatcag cttttctatc aaacctggag aaatggttgg
     5941 tatcgtcggg gcgactggtg ccgggaagtc aacactagca caattgatcc ctcgtctatt
     6001 tgatccgaca gaaggcaaag ttgaagtagg tggtgtagat cttcgtcaag taaatgaaaa
     6061 gagtttaaga caaacggttt cctttgtttt gcaaaaagca attttattct caggaacgat
     6121 tgctcaaaac ttgcgccaag ggaaaaagaa tgcaacagaa aaagagatgg aacatgcttc
     6181 ttctattgct caagcaaaag aattcatcga aaagttatct gatcgttacg aagcaccaat
     6241 agaagaacgt agcagtaact tttctggtgg tcaaaagcaa cgtttatcca ttactcgtgg
     6301 cgttatcggt aatccaaaga tcttgatcct agatgacagt acgagtgcac tggatgctcg
     6361 ctctgaaaag ctggtccgag aagcactcga tcgagatttg aagggaacaa cgacgattgt
     6421 catcgcacaa aaaattgctt ctgttgtcaa agcagaccgc atcttagtat tggatgaagg
     6481 cagattagtc ggcgaaggaa cccatgagga attggtagct ggaaaccgtg tctatcagga
     6541 aatctttgaa actcaaaaag gaacggagga gtaagtatga ctgatctaat aaaagcaagt
     6601 aaatttttct atcactattt gaagcggtac aaactctcct ttttctttat ctttatcaca
     6661 gtgatcattg ctacctatct acaagtaaag gcacctcaat acatcgggga agcttttcaa
     6721 gaattagcta attacgtagg aggcttgatg caaggcgtag acgataaaag caaatttgtt
     6781 tctgtaatat ggaaactctt gctgttttat gtactagcta gtgcagccaa ctttatctat
     6841 agtattttgt tcacacaagt agtagggaag tcaacgaacc gaatgcgtat cggtctattc
     6901 aacaaattgg aaaaattgac gatccgtttc tttgattctc atcaagacgg tgaaatttta
     6961 agtcgtttta ccagtgactt agataatatt caaaatagtt tgaaccaagc tttgcttcaa
     7021 gtcatcacga atgctgtttt acttgtcggg atcttgatca tgatgttccg ccaaaatgta
     7081 gagttagctt gggctacaat tgcttccact ccagtagccg ttttgatagc tgtcttgatt
     7141 atccgcaaag cacggaaata tgtggatctt cagcaagatg aagtggggaa actaaatggt
     7201 tatatggatg aaaaaatcag cggacaacgt gtcatcatca caaatggctt acaagaagaa
     7261 acgattgaag gattcttaga gcataatcaa agtgttcgcg aagcaacctt caaaggacaa
     7321 gcttattctg gtctactatt tccgatgatg caaggaatgt cactggtcaa tacagcgata
     7381 gttatctttt tcggcggctg gttagcatta aatggtgatt tggaacgttc ggtggcatta
     7441 ggcttagttg tgacattcgt ccagtattca caacaatatt accaaccact gatgcagatt
     7501 tcatctggat acagcatgat ccaattagcg attacagggg caagacgatt gaatgaaata
     7561 tttgatgaaa aagatgaagt caaaccagaa aacggtaaag catttgatgg tgttgaaaaa
     7621 ggtcttgcgt tgcagcacgt agattttggt tattctccag aaaaactagt cttgaaagat
     7681 gtctcgatca atgtggacaa aggagaaatg gttgctcttg ttggtccaac aggatcgggg
     7741 aaaacgacga tcatgaatct gctaaaccgt ttttacgatg tcaatgatgg ttcagtagtc
     7801 atcgatggaa tagatatccg agattatgat cttgatgcat tacgtagaca tgtaggaatc
     7861 gttttgcaag aatctgtatt gttctccgga acgatcaaag acaatatcac attcggcaaa
     7921 ccggacgctt cagatgaaga agtcatcaat gcagcaaaac aagcgaatat tcatgacttc
     7981 atcatgacgt tagaaaaagg ttatgataca gagatatccg aagaaaataa cttattcagt
     8041 acaggtcaaa agcagttgat cagtattgct cgaacgatca tcacaaatcc atctttattg
     8101 attttggatg aagcaacaag taatgtagac acggtaacag aagcaaggat ccaaaaagca
     8161 atggatgaag caatcaaagg aagaaccagc tttgtgattg ctcaccggct gaaaacaatc
     8221 ttgaatgcag atagaatcgt tgtcttgcgt gatggggaag tcattgaaga aggcagtcat
     8281 catgagttat tagcagaaaa tggattttat gcggaacttt atcataatca atttgtattt
     8341 gaaagttaaa ttaaaagaac ctcgttataa cggggttctt tttatatatt ttatgttgtt
     8401 tgtttttata aggggca
//
LOCUS       DANKHL010000127         8312 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000127 DANKHL010000000
VERSION     DANKHL010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.131"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(105..629)
                     /locus_tag="PW101_002007"
     CDS             complement(105..629)
                     /locus_tag="PW101_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2514401.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="PW101_002008"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="PW101_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDL2514402.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="PW101_002009"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="PW101_002009"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDL2514403.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="PW101_002010"
     CDS             complement(2351..3031)
                     /locus_tag="PW101_002010"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL2514404.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="PW101_002011"
     CDS             3157..4026
                     /locus_tag="PW101_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2514405.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="PW101_002012"
     CDS             4052..4567
                     /locus_tag="PW101_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514406.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="PW101_002013"
     CDS             complement(4614..5207)
                     /locus_tag="PW101_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2514407.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="PW101_002014"
     CDS             complement(5232..5411)
                     /locus_tag="PW101_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514408.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="PW101_002015"
     CDS             complement(5488..5997)
                     /locus_tag="PW101_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514409.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="PW101_002016"
     CDS             complement(6093..6473)
                     /locus_tag="PW101_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2514410.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="PW101_002017"
     CDS             6803..7552
                     /locus_tag="PW101_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HDL2514411.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7873..>8312)
                     /locus_tag="PW101_002018"
     CDS             complement(7873..>8312)
                     /locus_tag="PW101_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL2514412.1"
                     /translation="KKAEGSPCFLLYQTFPGIGALTAALLLGELGDITRFKTHKQLNA
                     FVGIDIRRYHSGKYTGQDRINKRGNPKARKIIFFTIRNMIRQQRAAPNHIVDYYYKLK
                     KQPIPKKDKVATVACMNKLLKCMHAMVRAHTEYDYAYAVSVDH"
BASE COUNT         2374 a         1714 c         1500 g         2724 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct atttcctacg
     7741 attagtcaag taaaaggtgc aagaaaaata gctcgtaaac gaaagcgaca aaatcaaagc
     7801 acaccaaata aagccccgtt cgtaaaaaat tttcgatctg ggcaaagaag tggtatactg
     7861 taagtggttt agttaatggt ccactgagac cgcgtacgca tagtcgtact ctgtatgtgc
     7921 cctaaccata gcgtgcatac atttcagaag cttgttcata caagcaactg tggcaacctt
     7981 atctttctta gggataggtt gcttttttaa tttgtagtaa taatcgacaa tatgattagg
     8041 agctgcgcgt tgctggcgaa tcatatttcg aatggtgaag aaaataattt tccgtgcttt
     8101 gggattacca cgcttattga tgcggtcttg gccagtatac ttacctgaat gataccgtcg
     8161 gatgtcgatt ccgacaaagg cattcaattg tttatgggtt ttaaatcgtg tgatatctcc
     8221 taattcaccg agcaataaag cggctgtgag tgcgccaatc ccagggaacg tttggtacaa
     8281 caaaaagcaa ggagagcctt cggctttttt ga
//
LOCUS       DANKHL010000128         8265 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000128 DANKHL010000000
VERSION     DANKHL010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.132"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..762
                     /locus_tag="PW101_002019"
     CDS             <1..762
                     /locus_tag="PW101_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2514413.1"
                     /translation="FKKKIGDEISPEAVQKEFILRDSFAKNQFQQLMADIPAEEIEWV
                     KQVVELAEDKLKVEFSPNIYLTLTDHLHYAITRAKENIQLPNPLLFETKKFYPKEYQA
                     AKEALLFVKQKTGVELSEDEAGFIAFHFVNSQQGNEDMQVTMTATTMVRDILSIISKF
                     FGIVFDEESLNYQRIITHLQFFAQRYLKGEGSDEQDEFLYALVQGKYPKAFHCVERIN
                     EYLRQTQKQEMGIAEQIYLTIHIQRVVSEKKSIQS"
     gene            883..2748
                     /locus_tag="PW101_002020"
     CDS             883..2748
                     /locus_tag="PW101_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2514414.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            2796..4205
                     /locus_tag="PW101_002021"
     CDS             2796..4205
                     /locus_tag="PW101_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDL2514415.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            4313..5014
                     /locus_tag="PW101_002022"
     CDS             4313..5014
                     /locus_tag="PW101_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2514416.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            5173..5652
                     /locus_tag="PW101_002023"
                     /pseudo
     CDS             5173..5652
                     /locus_tag="PW101_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            6500..7075
                     /locus_tag="PW101_002024"
     CDS             6500..7075
                     /locus_tag="PW101_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDL2514417.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            7075..7515
                     /locus_tag="PW101_002025"
     CDS             7075..7515
                     /locus_tag="PW101_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL2514418.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            7531..8232
                     /locus_tag="PW101_002026"
     CDS             7531..8232
                     /locus_tag="PW101_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2514419.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         2711 a         1372 c         1827 g         2355 t
ORIGIN      
        1 ttcaaaaaaa agattgggga cgagatttcg ccagaagcgg tccaaaaaga atttatttta
       61 agagattctt ttgctaaaaa ccaattccag cagctgatgg ctgatattcc ggcagaggag
      121 atcgaatggg tcaaacaagt agtcgaactg gctgaagata aactaaaagt tgaattttcg
      181 cctaatattt atctgacatt gaccgatcat cttcattatg cgatcacacg agcaaaagaa
      241 aatatccaac tgcctaatcc attattattt gaaacaaaaa aattttatcc gaaagaatat
      301 caagcagcga aagaggctct tttgttcgtg aaacaaaaga cgggtgtgga actttctgag
      361 gatgaagccg gttttattgc ctttcatttt gtgaatagcc aacaaggaaa cgaagacatg
      421 caagtgacga tgaccgctac cacaatggta agagatatac tgagtatcat cagtaaattc
      481 ttcggcatcg tgttcgatga agaatctttg aattatcagc gtatcattac tcatctgcag
      541 ttttttgccc agcgttatct caaaggggaa ggatctgacg agcaagatga atttctctac
      601 gcattagttc aaggaaagta tccaaaagcg tttcattgtg tcgaacggat caatgagtat
      661 cttcgacaaa cgcaaaaaca ggaaatgggg atcgcagagc agatttattt aacgattcat
      721 attcaaagag tagtaagtga gaaaaaaagc atccaatcat aaggattgtt actgtatggc
      781 aggcaaaacc tggattgaaa aaataggttc acgaatgttt gtgactgttt ttttcaatct
      841 gggtttttat tttttaaata aaaaagaaag aaggaaaaaa caatggataa tcaagcagtt
      901 ggtcgtcgtg tatgggaagc ggtaggagga caaaagaatg tcaaaagttt agtacattgt
      961 gctacgcggt tgcgattcag attaaaagat gaatctttag cagatacgca aaagttaaaa
     1021 gaagacccgg acgtgatcca agtcgtacaa agtggcggtc agtatcaagt agtgattggc
     1081 agtaatgtag cagatgttta tcaatcgatc gtggatgagg aaggtcttgc agtttcggag
     1141 gatggaaaag aagagtccaa aaatattttg aatcgtctga tcgatattat ctctagtata
     1201 tttacacctt ttctaggggc gatggcagcc gccggggtat tgaaaggatt cttatcattg
     1261 gcaacggtga tgggctggct gacagctgat agcggtactt atcaaatatt gtttgctgca
     1321 gcagatggtg tctttacctt cttacctgtc atgcttgctt ttacagcagc taaaaagttc
     1381 aaagccaacc agtttttagc agtagcaatt gctatggcac ttgtgtatcc agctatcaca
     1441 gcagttgctg gtgcgggaga agctatcagt ttctttggga tcccagtgat tctttcacca
     1501 tctggttata cttcttctgt cataccaatc attctagccg tatgggttca aagtaaatta
     1561 gagccattcg taaaaaaagt gattccgcaa tttctgcaaa tgattttagt tccgcttgtc
     1621 gttttagtag tgatggttcc attaacattt cttgctttag gaccaatcgg gacggttgcc
     1681 ggtaatgctt taggcggtct cttcaactcg atttatggat ttagtccaat cgtagcaggt
     1741 ctaatcatgg gaagtttatg gcaagtattc gtgatgttcg gcatgcattg gggattcgta
     1801 ccgatcatgt ttttgaatat cgaacaatat gggtttgatg tattgatgcc aatgcttctt
     1861 ccagccatct tggcacaagg tggggctgct ttagctgtag cattgcgaac aaaagatacg
     1921 aaacttcgag cattagggat ttcttctaca gtgacttcgt tatttggaat cactgaacca
     1981 actgtgtatg gtgtgacatt acctttgaag aaaccattca ttgccgcttg tatttctgga
     2041 ggtattggtg gagctattat cggattttcc ggagtaaaag ctttctccag cagtttggtc
     2101 agcttattga cgattccgac tttcattaat acggtggatg gtgtagagtc aaatgtgaca
     2161 gtagcagtaa tagctacagg aattgctttt gttcttgcat tcgtaggtac attgatttta
     2221 ggatttgacg aacaaacaca agacaatcaa ttagaaaaca aacatgcgaa tgcaggagaa
     2281 ccgattactt cagcacggca taccttaaaa agtcctttaa cgggtaaagt actgccattg
     2341 agtgaagtac cagaccaagt tttttcttct ggcgtgatgg gaaaaggtat cgctattgat
     2401 ccagaagtag gagaactagt tgcacctgca gacggagaga tcacgactat ttttccaact
     2461 ggacatgctg tagggatcac aacgactgac ggagcggaga ttttgattca tatcgggatg
     2521 gatacagtag aattaaatgg gaatggcttt gagatcttag tcaaacaagg ggatctcgtc
     2581 aaagcaggag atttattgat tcgttttgac atcgaagcaa ttagagcagc tggctatagc
     2641 gtaatcactc cagttgtcat tacgaatact gatgcatttg ccgatatact tgaattagac
     2701 caaaaagaga ttattgcaaa cgaagatgtt ctggcaattg taaaataagg aggaaatcag
     2761 gatgcaatca cgtttttcag aaaaattttt atggggtgga gcaactgctg ccaaatcaat
     2821 tagaaggggc ttatgatcga gacggaaaag gactatccgt tgcagatgct atgcctggag
     2881 gtaagcagcg ctttgcgatt atcggcagtg aagaatttga ctggacgatt gatcaagaaa
     2941 aatatattta cccgaatcat cgaggaatcg atcattacga tcgtttcaaa gaagatcttg
     3001 ccttgtttgc ggaaatggga ttcaaatgtt atcgtttttc gattgcctgg acacgaattt
     3061 ttccaaatgg agacgaaggt actcctaacg aagcgggatt agaattttat gatcagctga
     3121 ttgatgagtg cttaaaatat gatattgaac ctgtgatcac gatttctcat tacgaaatgc
     3181 ctcttcattt ggcaaaagaa tacggtggct ggaaaaatcg aaaactgatc gacttctatg
     3241 aacgatttgc tcagacagta ttagagagat acagcagtaa agtcaaatat tggatgactt
     3301 tcaatgaaat caattcggcc tttcactttc cagctttaag tcaaggatta gtaaaaagca
     3361 atggagcagg agagtaccaa aatattttcc aagcatggca taatcaattc gttgcaagta
     3421 gcaaagccgt aaaaatcggc cacgaattgc gttcggatat ccaaatcggc tgcatgatta
     3481 tctatgcaac aacttacagc atcgatgcca atccagtcaa tcaggcagca acaatgatcc
     3541 aaaaccaaga attcaatttt ttctgcacgg atgtccaagt acgtggagaa taccctgcat
     3601 acacagcgcg tacccataaa aaatatggag tcgatccgga aaaattagag caaacggaaa
     3661 aagacttcca gttgctaaaa gaatatccag tggactacat cggatttagt tattatatgt
     3721 ctacggcaat caatgagact gatccacaag cagcaacttc agaagggaac ttgctgggag
     3781 gagtaaaaaa tccgtttctt gaggcaagtg aatggggctg gcagatcgat ccagaagggc
     3841 tgagaatcgc attgaatgaa ttatacaatc gttaccagaa accattattc attgttgaaa
     3901 acggtcttgg agccattgat catatggaag aggatggcac gattattgat gattatcgga
     3961 tcgattattt acgtcgtcat atcgaagcga tggctaatgc agtagcagac ggtgtagatt
     4021 tgatgggcta tacgccttgg ggatgtatcg atcttgtcag tgcatcaaca ggagaaatga
     4081 gtaaacgtta cggctttatc tatgtggatc tagacgatga aggaaatggg acactgaatc
     4141 gatcaaagaa aaaatctttt gattggtata aagaagtaat tgcctcaaat ggcgtaaact
     4201 tgtaattaaa gcagctgaat gaaaaagaac acacttctca catgtgattt gtgttctttt
     4261 tttatttttt atgtgctatt cttttagtaa tagtaaagca aagggtgacg ttatgaaaag
     4321 gacacgtgta ctatacttgg aagtcgcaga taaaattaaa gaagatattt tctcaggaaa
     4381 atatccagtc ggttcgatgt tgccgacaga aagtgaatta gaagaactgt ttcaagtcag
     4441 caaaatcact gtccgaaagg caatcgaact gctggcatca gatgaatatg tcgcaaagaa
     4501 aagtggtcgt gggacgacgg ttctaagcaa tcgtccttac aatcgattat ccaaagcggc
     4561 aagctttacg caaatcttgg agaactcccg atatgaagtc cggaaagaaa cgctggattt
     4621 gcaattggtc catataaaga aggaagatcc tttgttttcc tttttcggtg agcaagctgt
     4681 ttgttttaag cgcttatatt atttgaacaa tcagccttat atttattttg ttcattatct
     4741 tccacaagaa atgatgcaat tcagcagaga agaatttggc aaagaatctt tgtatcgttt
     4801 gctgacgaaa aaaggatata ctattgactc attttctgat gatttctcgg cagtattttt
     4861 aacagaagaa gaacaaggaa ttctgaaaac agacgcacag atcggcatca aacggatacg
     4921 gaaatcgtct acgcaaattg gggaaatgat tgaatattcg gaggcggtct accatacgga
     4981 gctgcatcct taccatattg attatgaaac ataaataaaa aaagaggttg tgaaaaagct
     5041 gccctgcatc aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcgtttgg
     5101 ttaggcttat taccgcagat acacggctgt ttctggatta ccatttattc tggataaggg
     5161 ggtgtggcac gattttcaga aggaatctat gaaccaatca ctgtattgat tttagcttcg
     5221 atggtaaccc cattcggctt gacgatcgct tatttcttag ggaaaatcat tcgtaaaaat
     5281 atactcaatc gtcaagaaat cgatacattg aagaccgctt ttcctatggg gatctgtcag
     5341 atcacagagg gatgtttccc tatcgtattg aacgacttgg cacgcaatgt catagcaacg
     5401 ggagtcggtg ggacagtagg tggcggatta agcatgtttt ggggcgcaga tagccgtatt
     5461 ccggcatcgg gaatgtttgc agtcgctacg atgaccagac catgggcatt cattggtgca
     5521 ttgttagctg gttcttttgt cacaggaata accattttat tattgaaaaa accagtagat
     5581 ccgaatgcag aaatcatcca agaagaaaaa gaggaagaag acatatcttg ggatgatttg
     5641 acaattagtt aggagaggca gaatggatta caagaaaaaa gtacaataga tgtaccttga
     5701 gacaggaatg gtatgaaaga atcagagtta gaaagaatcg aatatacaga ttttggttta
     5761 ggagcgatcg aaactgtcca gagcatcggc accctccttg aaatgacgat gaaggaaaaa
     5821 tgagaacctt tcgttgtcga aagggtctgg tataccttat gtcgaaggaa aggaaacaaa
     5881 aaaactttcc tgtactattc caaaaaggaa agaaagttat tatacagtaa aaaaagaaat
     5941 tattcttcat gctagagatc agtacacgtt cgagcccaat atcaaacact agttccaggc
     6001 aggcgcaaca ggtgcggtta tcactgaatt ttcttccagc agtgacgatt caagtgatat
     6061 ttttacaaat ccagctattt atcgttaaca aaaagatttt taggagaagc tgatgaacaa
     6121 aaatgatcaa attttgatcg tttagttcgt cggctttttt atttagagaa acatcattta
     6181 tcagtgaaac ataacacgag aacaaaatga ttttttcgtt aataaaagaa acaagtattt
     6241 gttgtttaaa ataatacacg ttattggcat ataggacgaa atcccagtat cttctataag
     6301 taagtcattt catatcgatg aaaacacgtt atttattttt gttttatcta taaaatggct
     6361 gaaaaatgat ttttttctaa atactatata tagtggcgtc tatcctatat accactaaaa
     6421 aactacaaaa tctagtcttt tattttaaaa aaacacgtgt agaatgaaga tagacttttg
     6481 agttagggag tgttttttta tgaaacttac tgttttttgc ggttcccgtt ttggtaataa
     6541 agaaagttac aaatttattg cgcaaacgtt aggaaaatac atggcacaag aagaaatcga
     6601 actggtttac ggtggttcat actccgggat tatgggagtg attagtaaaa cagtactgga
     6661 aaataacgga aaagtcacag gtatctatcc gaatgggctt tttgaaactg aattgcctgg
     6721 aaaagatgtt actacattta ttcctacaga aacgatcgat gaaagaaagg tattattatt
     6781 tgaaaaggga gatgctgtcc tcgttttccc tggaggtcta ggtacattgg aggaattctc
     6841 acaacttctt tcttggatag caatcggtct gacgcccgac aagccaatcg gaatattgaa
     6901 tattggcggt tattacagcg gcttgcaaca attattagaa acttttgcaa aagaggattt
     6961 tatggataaa aaatggttgg atcaagtaat cttttccaac aatccattgg aactggtcaa
     7021 tgaattacga gcagtatcta ttaaaaatca gttattattg aaggaggcaa aataatgagt
     7081 caaatatatt tagcaggacc atttttttca gaggagcaaa tcgatcgagt aagtcgtatc
     7141 gaaaaagcgt tggaggaaaa caaaacagtc acaagtttct actctccacg tcatcaccaa
     7201 gaaagcaatt atgaactatt cagcgctgga tgggcgcagg aagtatacga aaaagatatg
     7261 gaagaactga caaatgcaga atttgttgtt gctatccttg actttgagca tcaaacaatc
     7321 gatccaggaa cggcttacga attaggtgtg gcaactatgt tgaaaaaacc gatgatcatt
     7381 gtacaagaag agacggtacc tacgaacttg atgattacac aaagtctcca tacgtattta
     7441 aaatcagacc aagctgtacg tgaatatgat tttgaaacat tgcctgttga aacttacgta
     7501 ggcgagtact tatagaagag gaagggttgt atgtcagtga ttttgatttc aggaacgatt
     7561 ggtgcaggta agagcagttt gacggatatg cttgcaaaag agattgattc taaaccattt
     7621 tatgaaaatg tcgaagataa cgaagtattg ccgctctttt attcaaatcc tgagcagtac
     7681 gcttttttat tgcaaatctt ctttttgaac aagcgatttt tagcaatgaa aaatgcttta
     7741 gtgaacgatg acaatgtgct agaccgatcc atctatgaag atagcctgtt gttccatcta
     7801 aacgctgatt taggtcgggt aacggatatc gaagtacagc agtacgatag cttattagat
     7861 acgatgctaa acgagttaga tgatgttgct ccaaaaaaac ggcctgattt gatggtccat
     7921 atcaaagttt ctttggacac gatgctagaa cgtatcaaaa aacgtgggcg tgactatgaa
     7981 cagctagaaa gcgatgagac gttatatacc tattatgaaa cgttgaacac ccgctataat
     8041 caatggtatg aagattttga tgtatgtcct aaaatacaag tggatgggga taaattcgat
     8101 tttgtagaaa atccagaaga tgcaaaaaag gtgatccgtc agatccaaga aaaattagct
     8161 gaattggaag gaaagcctgc tggaaaatat tttacggata accagcatgc aaaaggtcaa
     8221 gaattaggct aaaaataaaa aaccaaacga cgggaaagaa aaatt
//
LOCUS       DANKHL010000129         8249 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000129 DANKHL010000000
VERSION     DANKHL010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.133"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(204..1652)
                     /gene="cls"
                     /locus_tag="PW101_002027"
     CDS             complement(204..1652)
                     /gene="cls"
                     /locus_tag="PW101_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL2514420.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1804..2697)
                     /locus_tag="PW101_002028"
     CDS             complement(1804..2697)
                     /locus_tag="PW101_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2514421.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="PW101_002029"
     CDS             complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="PW101_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDL2514422.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3472..4573)
                     /gene="prfB"
                     /locus_tag="PW101_002030"
     CDS             complement(join(3472..4500,4502..4573))
                     /gene="prfB"
                     /locus_tag="PW101_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDL2514423.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4699..7233)
                     /gene="secA"
                     /locus_tag="PW101_002031"
     CDS             complement(4699..7233)
                     /gene="secA"
                     /locus_tag="PW101_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDL2514424.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(7455..8006)
                     /gene="raiA"
                     /locus_tag="PW101_002032"
     CDS             complement(7455..8006)
                     /gene="raiA"
                     /locus_tag="PW101_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDL2514425.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(8134..>8249)
                     /locus_tag="PW101_002033"
     CDS             complement(8134..>8249)
                     /locus_tag="PW101_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDL2514426.1"
                     /translation="KILIIDDVYTTGQTLFHAADCLQNAQPKIIRTISLAR"
BASE COUNT         2200 a         1816 c         1440 g         2793 t
ORIGIN      
        1 gctatactgg ctttgaaatg tgtctctttt aatagcatat attagcttat ttcttgcttt
       61 ccccaaaaaa caagagatac cccaaagtta atttcgctca tgcgagacgc tttctccatt
      121 tctgacgtac tctcagaaat ggccccgtta aaaaaggaca cgagatctcc atctgttgat
      181 ggttctcatg tcctttttat gttttaaagg agcggcgaaa tcaatcttgc taatccttct
      241 ttcatcttga tgattattcc tcggttttca tacatttctt ttgtgagatg cgtagaaatt
      301 ttcgagtctt caaaaaaagc ttttcttaca cgtgaagcca ttcgttcatc ataaaccagt
      361 gtattgacct caaagtccaa gcgaaatgaa cgaacatcga tatttgctga gccaactgaa
      421 acaatgccac catcgataat catcgtcttt gcatgaataa atccattctc atatgtttcg
      481 atccttgctc catactcgat cagctctgca gcgaaagaat acgttgccca gtaaacgaga
      541 agatgatctg gtttgttagg tatttggata tgaactttta cgcctgaaag aagagccaac
      601 ttcagtgctt catgaatcga tccatccggt atgtaataag gcgtttggat caatatctcc
      661 cgtttggcta acgaaatcat tttcaaataa gtcattttga tttgttcatg ttcggtatct
      721 ggtccgcttg tcacgatttg aacagctata tcaccagtag tgtgaatgga cggaaaaaat
      781 ttgggagaat agcctacttc ttttgtatgc tgagaattcc agtccatcaa aaaacggttt
      841 tgcaaggtat acaccgcttc tccgtaaata cgcaaatgat tgtctcgcca ataaccaaat
      901 ttctttacca atcctagata ttcatttcca acattgaacc cacctgtata tccgatttcc
      961 ccatcaataa ctacgatttt acgatgattg cgataattga tccgaggatt aatatacgga
     1021 acaaatagag gaaagaaaaa tgcgacttct ccgcctgctt ttttcaaatt ctgaaaaaag
     1081 ctggaagaaa cttgtgtgga accccatgca tcaagaagga ctctgacttt tacccctcgt
     1141 cttgccgcct ctgtcaatgc atctcgaact tcttcaccta atgcatcact gcgataaata
     1201 taatactgga agtggatatg gtcttctgcc tgcttgatat cttcaatcag cgcatcgaat
     1261 ttttcacgac catctgtaaa taaagtgatt tcatttttag tcgtatagag cgtactttca
     1321 aataccgtga gcatatagat caattgtttc acatctactt gtccagtagg cggatgagga
     1381 taaagccctc gcatcaatgc ctgtttgtct tcttcaattt ccaggttctt accgattttc
     1441 ccttggatct tcaaatcaaa gattttctct ttggaaatcc ctcttcctaa aaaaatatat
     1501 aaaataaatc ctacaactgg aatgaacatc aaaacgagca gccatgccca tgtttgagca
     1561 gtttcctttc tttctcgaaa aacgatgata aaggaaagca aaatattcat aatcaaaagg
     1621 atcgtaaaaa aattgtcaac gataattttc acggcttcac ctcttcttct accaacttaa
     1681 gaatacccaa gttgtttttt aaagtaaagg gaaaaaagcc gcttccttct attctcctag
     1741 ccaataaaaa aagaaagctg gaacgaaact gtccaacttt ctcattttat ttatattgtg
     1801 gtattagaat ttcaagaaac gtctcataga aatgatcgag ccaagtgaac caatgatcac
     1861 accaatgccg accattaaaa gattgatttt ccaaacgata ttttgtggat gaatcaatga
     1921 ataatgggaa cgtaataatg atggattgat caattggaaa aaccatgcat aaccaaatgt
     1981 gatgatcaaa attggaagaa ttgcaccgat cagaccgatc cagccacctt caaggaaaaa
     2041 cggccagcga atatatccat tttttgcacc gactaaacgc atgatttgaa tttcacgctg
     2101 tctagacaaa atcgtgatcc gaatcgtgtt cgaaatgaga aacatcgcta cgaacagcag
     2161 caatgccgct gcaactaatc cccatgtccg aactgcacca gcgatttgga aaatccgatc
     2221 tgaggaagct ccaccgtaat cagaacgaga aacattcttc aaatctgctg cttcttctgc
     2281 tacttgtttt gtataagaag gatcagtagc acttactaca tacacatcat ataacgggtt
     2341 attgtcacct tcaaacaatt tccaggaatc acccatttcg ttttggattt ttttcaattc
     2401 ctgttctttg cttgaaaatt caactttttt aacatgatct aagtcattca attctttttc
     2461 tagctttttc atctcttcgt cttttgtacc gatatctacg aatacagata catcaacgtt
     2521 tccttctata tcctgagcta atttagttgc attcatgata actgccatga atacaccgac
     2581 taaagtcaac gtgatcgtta ccgcactgac tgaagcaact gtcatccagc cgttacgttt
     2641 caaactcttg atactttcta ataaatggcg gaaaaaggtt ctaatcatcg tatccatatt
     2701 ccccttccgc ttgatctcga ataatgcggc cgttttcgat agcaattact cggtgccgaa
     2761 tcgtatttac gatcgtactg ttatgtgtag ccatcacgac agtagttcct tgggcattga
     2821 tccgatctaa taatttcatg atttcccatg agttttctgg atccagattt cctgtcggtt
     2881 catccgcgat aagtacttta ggcgtattca cgattgcccg agcaatagag acacgttgct
     2941 gttccccacc ggataattcg ctaggaaaga ctcgaacttt gtgtttcaaa ccaacaaggt
     3001 ccagcacttc cattactcgt tttttgatat ctcgaggttt tctgccgata acttgcatag
     3061 cgtacgcaac attttcatat actgtttttc ttggtaataa tttatagtct tggaacacca
     3121 cgccgatttc tctgcgtaga taaggaactt cgcggttttt gattgctatt agatcatgac
     3181 ctgcgacgtt caaagttcct tttgaagctt tttcttctcg atacatcaat ttgatgaatg
     3241 tagatttacc ggctccagac ggaccaacca catagacaaa ctctccttgt tcgatgacaa
     3301 tcgagagatt gcggatagcg gtcgtaccat ttgagtactt cttcattaca tccttcattt
     3361 caatcatggc gttctctcca tatctttttc agtctttagc tattatagca tggcaatgtt
     3421 tcaacatgct tgcaaaatct tacaatttta tttcaattta acaatgggtt cttattgtag
     3481 atgcattttt aaatagccgt caataaataa atcaaggtca ccgtccataa ctgcttggac
     3541 atttcctgtc tctacgtttg tccgatggtc ttttaccata gaatagggat ggaaaacata
     3601 agaacggatc tgtgaacccc agccgatttc tttttgttct cctcgaagtg cagctgcttc
     3661 ttgttctttc ttttccactt ctaattgata aagcttagct tttaacatcc ccatcgcttg
     3721 ctcacggttt ttcaattgtg agcgttgtgc ctgactagct actactgttc ctgtaggcaa
     3781 gtgagtgatc cgtacagcag attctgtttt attgatgtgc tgaccacctg caccacttgc
     3841 tcggtacgta tcgattttca aatcatctgg attgatttct atatcgatcg tttcatcaag
     3901 ctctggcatc acatctaccg aacaaaaaga agtatgacgt cgtttcgctg agtcaaaagg
     3961 cgaaatccgt acaaggcgat gaacaccttt ttctgatttt agataaccaa aagcgttatg
     4021 tcctttaatc agcagggtca cactcttgat ccctgcttca tcacctgctt gataatctag
     4081 tgtttctact tgatagccat gctgctctgc ccaacgtgta tacatacgta aaagcatgct
     4141 gccccagtct tgggactccg ttccgccagc ccctggatgc aattcgacga tcgcattgtt
     4201 tcgatcataa ggtccgtcta acagcataga taattcatac gtcttcattt tttcatctaa
     4261 tgcattgata cgtgtttctg cttcttcttg cattgctgca tcaggttctt cttgcagcat
     4321 ctccaccatc aattcgattt cttctagttc ttcggcaaga gaatggaatt ggtcataggt
     4381 ttctttatta gcgttatttt cgctgatcac tttttgtgcg gcttcgctgt catcccaaaa
     4441 accaggttca gccatttttg cttctgcttc cgcgatgtct tcttccagct gatctaagtc
     4501 aaagagacct cctgaaactt gtgacctttt cctgcatttc tgtcaggaga tttcgaattt
     4561 cggatatttc catgtttttt ccttctttca ctatactttt ttcattatat ctagtctaac
     4621 agaatcagca aataacagaa agaaggtgag acgaattctt aaaaaaagaa tttgtctcac
     4681 caatcctgct gtcagaaatt agtttgcatt ttttccatga cagttcttat attttttccc
     4741 gcttccacaa ggacaaggat catttcttcc gaccttattc acatggattg gctgtttttt
     4801 cgcgctagta ttactatttt ctttggcatc tccgtcttct gagtgagaag ctgttccttg
     4861 agaaacctgt tcacgctgta cgttttgacg gatctcagct ttcataaaga ttcgagtgac
     4921 ttcatactcg atggcgccta ccatatcttc aaacatctta tagccttctg tttgatattc
     4981 gactaatgga ttgttctgtc cataagcacg taagccgatt gactgacgta attgatccat
     5041 tgcatcaata tggtctgtcc atttagagtc cacaacacgc aagatcacga ctttttcaaa
     5101 ttccaacagt tgctctggac catttagttg agccgctttg gtattaaata cttcttgtgc
     5161 tcgttcgttt agatactctt tgatctcctg agctgttttt ccttcgatat ctgcaagagt
     5221 aatgctatct tcatggacaa gagtactgcc ggcaaaatcg atgatacttt ccaaattcca
     5281 attttctttc tctaattgtg tatgcgcatc tactacacga ctgattgtcc gtttgaccat
     5341 gttcattagt ggcttggata agcttttttc ttccatgatt acttcttgtc gctgtttata
     5401 gattacttca cgttgttcac gcatcacatc atcgtattgt aagacgtttt tacgtgtgtc
     5461 atagttattt ccttctacac gtttttgtgc agattctact tgtttcgtca gcatcttgct
     5521 ttggataaca gcatcttcct caccgatctt catccgatct aagaaagctt tgattcgttc
     5581 tgaaccgaat cgtttcatca aatcatcttc caatgacaag tagaactgtg acataccagg
     5641 gtctccttga cgtcctgcac gtcctcgaag ctggttatcg atacgacgag attcatgtcg
     5701 ttctgtcccg ataactgcta gaccaccaag ttctcttacc ccaagtccta acttgatatc
     5761 tgttccccga ccagccatgt tggtagcaat cgtcacagca cctttttgac cagcattcat
     5821 gatgatctct gcttctttaa agtgattttt cgcattcagc acttcatgag gaatacgttc
     5881 ttgattcagc atatctgata ataactcaga tgtttcaacc gcaacagttc cgactaacac
     5941 aggttgacct ttgcggtagc gatctttgat atcttgcacg acagcgtgga acttgctttg
     6001 taatgttggg taaagcaaat ctggacgatc atcacggata accggacgat ttgtcggaat
     6061 ctggtatact tggatgttat aaatttctct gaattcttct tcttctgttt tagctgtacc
     6121 agtcattccc gcaagtttct tgtacatacg gaaatagttc tggaaagtga tcgtcgccat
     6181 ggttttcgtt tcgtcttcga tctctacgcc ttctttagct tcgattgctt ggtgaagtcc
     6241 atctgaataa cggcgtccat ccatgatccg accagtgaac tggtcaacaa tcagtacttt
     6301 tccgtcttgg acaacataat cgatatctaa aagcatgata tagttggctc tcaatgcttg
     6361 atccaagtga tgggtcaatg cagtattttc aatgtcgtat aagttatcca aaccgaatgt
     6421 ttcttctgct ttttcgatcc ctgcttcggt taaactgatt gtttttgatt gtacatcgat
     6481 tttataatct tcttcttctt tcaaacgttt cacgaagttg tctgctcttg tatataaagc
     6541 tgtggatttt tctgcttgcc ctgaaatgat cagcggcgtt ctcgcttcat caatcaagat
     6601 tgagtcaact tcatcgacaa tggcataatt cagcggacgt tgtaccattt gatggcggta
     6661 aacgaccata ttgtctctca agtagtcaaa tcctaattca ttattcgtac tataagtgat
     6721 atcacaatta taagcctcgc gtttttcatc cgaagatttt gagttgatat tcaaccccac
     6781 tgttaatcct aagaaattat aaagttcgcc catttccgtc gagtcacggg ttgctaaata
     6841 ttcattgacg gtaactacgt gtaccccttc gccagtcaga gcatttagat atactggcat
     6901 cgttgcggtc aaggttttac cttcaccagt acgcatttca gggatatttc catcgtgaag
     6961 cacgattcca cccatcaatt gtacgtggta tggatataaa cctaatacac gttttgctgc
     7021 ttctcggacg actgcaaaag cttccggaag taattgatct aatgtttctc ctttttggta
     7081 tctgcccttg aattcagcag tttttgcctg taattgttca tcagttaatg ctgccatttg
     7141 gtcagcgtat gcttcaactt tttttgcaat gctatctaaa cgttttagtt ctttcttatc
     7201 gttttcgata agtgaacgaa tcaaattggc catgcgttgt gtaatctcct tttccttctg
     7261 tgtattatcc agactatgta gactttactt ttcattttat cattagttat agataattga
     7321 aatatcttaa gcgtattaaa gtaagaactt cataatttat tcatatttca tctaaaagag
     7381 gaggaaactc aaaaaaactt gcggcagctg tcagtagctg ctgcaagttt tcgttgttat
     7441 ttaagtaagg ctgatcagtc tgtttcaatc agtccgtatt ttccatcttt acggcggtaa
     7501 acgatacttg taccatttgt ttctgcatct tcaaaaataa agaagttatg tcccagcatg
     7561 ttcatttgta agactgcttc ttcactatcc attggtttca atgaaaggcg tttcgtacgt
     7621 acgatatcta attcagaatc attttcgttt tcgtcctctt cattgaataa agcagcagca
     7681 tctgcagtat tcatgcccgt ttcacgggat ttacgattaa tttttgtttt gaatttgcgg
     7741 atttggcgtt ccaatttgtc aacaaccaaa tcgatacttg cgtataaatc tggagaagtt
     7801 tcttcggcac gtaatacaag ataaggtaat gggatcgtta cttccacttt tgctgttttt
     7861 tctgtataaa cttttaagtt cacatgcgcc gttgcatcgg gtgcgtcact gaaatagcgt
     7921 tctagtttgc ctactttttt ctcaacgtag tcgcgaatcg cttcagttac ttcgatattt
     7981 tcgccgcgta cattatatct aaacataaaa attacccctt tcgtctacca caatcaagaa
     8041 ggagaaggac cactctttcc ctcttcttaa ttctattata tcgaactcta ggttatttgc
     8101 aaattaaatc gctatcaaat ttccacatct gattcatctt gccaaagaaa tggttcggat
     8161 gattttcggc tgtgcgttct gcaagcaatc tgctgcatga aaaagcgtct gtcctgttgt
     8221 atagacatcg tcaataatca atatttttt
//
LOCUS       DANKHL010000130         8245 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000130 DANKHL010000000
VERSION     DANKHL010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.134"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            20..493
                     /locus_tag="PW101_002034"
     CDS             20..493
                     /locus_tag="PW101_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDL2514427.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            502..729
                     /locus_tag="PW101_002035"
     CDS             502..729
                     /locus_tag="PW101_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2514428.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1365..1736
                     /locus_tag="PW101_002036"
     CDS             1365..1736
                     /locus_tag="PW101_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514429.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(1992..2234)
                     /locus_tag="PW101_002037"
     CDS             complement(1992..2234)
                     /locus_tag="PW101_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2514430.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2266..3168)
                     /locus_tag="PW101_002038"
     CDS             complement(2266..3168)
                     /locus_tag="PW101_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2514431.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3181..3369)
                     /locus_tag="PW101_002039"
     CDS             complement(3181..3369)
                     /locus_tag="PW101_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL2514432.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3383..3946)
                     /gene="amaP"
                     /locus_tag="PW101_002040"
     CDS             complement(3383..3946)
                     /gene="amaP"
                     /locus_tag="PW101_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL2514433.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(3984..4877)
                     /locus_tag="PW101_002041"
     CDS             complement(3984..4877)
                     /locus_tag="PW101_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2514434.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4955..5878)
                     /locus_tag="PW101_002042"
     CDS             complement(4955..5878)
                     /locus_tag="PW101_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2514435.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5927..6277)
                     /locus_tag="PW101_002043"
     CDS             complement(5927..6277)
                     /locus_tag="PW101_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2514436.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6310..7212)
                     /locus_tag="PW101_002044"
     CDS             complement(6310..7212)
                     /locus_tag="PW101_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL2514437.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7205..8062)
                     /locus_tag="PW101_002045"
     CDS             complement(7205..8062)
                     /locus_tag="PW101_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL2514438.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
BASE COUNT         2262 a         1589 c         1398 g         2996 t
ORIGIN      
        1 agataaaaga gggataaaaa tgaaatttaa aacattgcta ttaaaatttg tcgttttagg
       61 tataagtgcc gtatttattt tgttcggcgt acttttttat actcagatcg ctacaagcga
      121 aaaaggattc aattttgatt ggcagaccac attatttgtt tctgtcattt actttgccct
      181 tctcctaggg atcgttattg cttatttctt attgaaacta ttgaaaaaaa tcgaagataa
      241 ccaagctttt tccactcaaa gcttaaacat actaaaaaaa atcgaacgaa ccattttgtt
      301 tattggtttc gccttttttg gcttacttcc taaatttatg aaagtagcgg atttagacga
      361 tgctccaggt ttgatgttgg taggaatcgc tgtcgtattc attccttttg cagtatacac
      421 attaacaagc gtgctggaaa aactgcttga acatgcgatt ttacttaaga cagatcatga
      481 tttgacagtg tgaggtgcga catggcaatt atcgttaatt tagatgtagt attagctcaa
      541 aaaaaaatga gtgtgactga attgagtgag aaagtgggca ttacgatggc aaacatctct
      601 attctaaaaa atggaaaagc aaaagccatc cgtttttcca ctctcgaaaa aatctgccaa
      661 gttttggagt gtcaaccagg tgatttgttg acctataaaa atgactcgaa tactatacca
      721 gaagaataaa ataaagtaga acaaacagta aataaaaaat aatctgaatg atagatgtgt
      781 gagactttta gaagttattt acgtctatta ttcagatttt ttatttcgat tgaataccgt
      841 tttatctata aataagatat gtactgactc atacaaaggg ggccttttct tctccatgta
      901 ctatcacttt tcctgataca actggtttcc tgttacgttg tagttggatc tgcttttagt
      961 ataatacttt attcattcca ccagactgat agcagtttga gcagtctaaa taatcatgta
     1021 gtattacagg ccattctgtt tcataccttg aagtgcttaa aacttcagct aatggcccat
     1081 tgtgttaact cttactccat tcttttttag taagctccca tatatactgt aaagccttct
     1141 atatattatg tcatgttgct ttttaacatt tactatgtag ttttgataaa atgatttgaa
     1201 taacagtgat ggacaattct tttttatttt taacattatt ttgataataa tgattattta
     1261 tttaaaaata cattagataa catttgttac gacacgaaaa agcttcacaa acaaatatcg
     1321 ttattttaaa atgagggcga actcacacaa ttaggaggaa tagtatgcca tttattgttt
     1381 acttttttct ttcattactc gtgatctata ttccattacc aacatttttc ctgctcgtca
     1441 atcgtctagc agagcaacat gatacgattg ctgctcttac gaaactttgt ttacttctat
     1501 ttgtttgtct agattacttt acgagctatt attggctaaa acagacaaga gtaaaaaaga
     1561 gatattattt cttccttgtc tttttcaatc tctttgagtt tctgatccat tgctttttac
     1621 ttcatcagta tcatacgatc catttgtgga tcatctgggt tttcaaagtg gtcgtattat
     1681 tttgtatgtt cacctttcct atctcaagaa aatttaggga ggcattgttt tattaaagaa
     1741 gcttctctgt atcaataaag acacatttaa ggaaaaactt ttaaaaatct aattgggaat
     1801 ttgcccgcat aaataagagg ctgtgacaaa agtcttgtca cagcctctta ttccgtataa
     1861 acagtgttca aaagcttgca tcttcgataa tatgcccaac caaatgaaaa atatgagaag
     1921 ctatttttgt ttggctgttc ttattacttg atgaagaaca gctttttcac gacctctttt
     1981 taaaagtctt tttacagttt tcgagcaaag aatgatacga ttagtactaa aatgatggca
     2041 ccaatcaatg atggcacgat tgccattcct gcaatacttg gtccccaaga acctaataat
     2101 gcttgaccaa gtgctgatcc aactaaacct gctacgacat ttgcaataat ccccatcgaa
     2161 gaacctttat tcgtgattgc tcctgcaatt gctccgataa ttcctccgac gataagtgac
     2221 caaataaatc ccatgtttcg ttcctcctaa tgttttactt ccttattact gaacccgtgg
     2281 ttctgtattt tcttttactt tttgtgcgcc gcttccagct gctgatttta ctttatctgt
     2341 ttgattcgaa gcaaattcac cggttttttt cgctgcgtca gttactttgt cttgcaccgt
     2401 ttcttgatct ttttcaaatt cttcttttgt tttgatgtcc actacgttag cgttgacttc
     2461 gatcacttct aaatgtgtca tattttttac ttctttgccg attacttctt gcatttcttc
     2521 aaagattttt tcgatatctt tgccatattc cacaatgaca tccatatcaa ctgctacttg
     2581 ttttttaccg acttctgttt cgatcccagc cgttacatta tctgtattca caagttttcc
     2641 tgccacatta gagaagaaac caccgtttgc agataataaa ccgtctactt gttcaattgc
     2701 gattccgatg attttttgca caactttatc ttcataattc agctcaccgc gcattgaatc
     2761 ttcctgttca gaatcatgct ttccttggtt atttgtatga tcagaaccaa gttttccttg
     2821 tccgtttgtg tggtcagaac taagtttttc ttgtgctttc gcatggtcag aaggatgttt
     2881 ttgctcttcc acaggtttag aaggatcgat tgcatcatgt tgttctggaa ctttagattc
     2941 tggagttcct ggatgatgag gtgcatgctt ttctgcatca tgtttcttct cttctactcc
     3001 ttgttgaacg ttagggacta ctggatctgg atgtgcagat actggtgctg ttttgttctt
     3061 ttcatcgttc acaccatttg attggtttgg tgtcgttggg tctggatgtg cagacactgg
     3121 tttttggttg ttcattttat tgttattatt tttgttattg ttattcatgg atgtttcctc
     3181 ctaagattta aaatagtgat ctaagatttt cgtttgtttc agataaaaac cgatagcgac
     3241 gccgatcgct gccatgatca agatcaataa ggtcttgaaa aagcccacgg tgatccataa
     3301 aacggctaat atcaatccaa gtaccccaaa gatgataggg agcttgtatg tcgaaaatag
     3361 ttctttcatt tttggtcccc ccctattcta ctcgtttgcg gtcattgctt cgtttagctg
     3421 gttcttcaaa gcctgtgtat ttcaccgata ctttcactgg ctcttttgtt cccaatatgt
     3481 gttgtacttc attttcgatt tctttcatca atgttcctgt tttaccaatg agtgctgaag
     3541 ttcgtttcaa ctcccctttt acattgacgg cgatcttgtt tttcgttgct ttgacattta
     3601 cttttggtga atggatgaat tcatcttcat gcaagtgaga acgaaccatt ccttcaattg
     3661 cttttttatc caacgtcaac tccccatcac catgtttcag tagaaagttt ccgattgttt
     3721 ttggataaaa caggaaaaca agtaacgtga taattgtcaa aatagcaaat gcaacggcaa
     3781 gccaaaatac atattgttgc acgtatgaat tagtgatgga aaagaatcga aacttctcta
     3841 gctgaaaagg caatggatag gttgcactta atagtatcgc aaacaaaagc acagatagca
     3901 aaagtaatgt tccgatcact ccgatcgttt ttacaccttt attcattttc gcacctcctc
     3961 ataggggtga aacgatcaac ttcttataag ttgatcgctt ctccaccagt tactccgtaa
     4021 atttgggctg taacataact tgctttattc gatgctaaga aaacataaac tggtgctaat
     4081 tctcctggct gacctgcacg ttctagtaaa ctttgctgac caaattcagg gatcgaatca
     4141 gaaggttgtc cttcgtctag ttgtaatggt gtccagattg gaccaggtgc tacaccattt
     4201 acacggattc ctttaggtgc aagctgtttt gctaaaccga tcgtaaagtt agcgattgaa
     4261 gcttttgttg ctgcgtaatc taataaatgt tcgcttggat tgaatgcttg aacagaagtt
     4321 gttgttacga ttgctcctcc tgctgggata tgcggaacag cttctttcac taaagcaaac
     4381 atagcgatga tgtttatcat aaatgtgtct ttgacttgct caagtggtaa ctcagcaata
     4441 gactgacaag aaatttgctg tcctgcgttt agtaccatcg tatctagccc gccaagttca
     4501 ctcacggctt tatctatgat ttctttaggt gcttgttcgt cacgaagatc aaaaggtagt
     4561 aagacaatct tacgtcctgc aactttcacg tattttgcta cttcctctgc atctttttct
     4621 tctcctggga agaattgaag agcgatatct gccccttctc tagcaaatgc aatcgctgct
     4681 gcacgaccga tccctgaatc tccaccagtg attagtacac ggcggttttc tagttgatta
     4741 ttgccttcat aggattcttc tccacaatca ggaacaggaa tcatattctg ctgtaaagct
     4801 ggtgtatcct gatcttgttt aggaaatttc tccgtatggt acaagtttcg cgggtcgatt
     4861 aattttggtt ctgtcattgc tgtattcctc ctagttttta aattgtattg ccaaaacaaa
     4921 tttctttgtt tgatacctgt tttaaataga ttctttaatt tgtataatct ttccggtata
     4981 aatggtaacc taaaactact gctgctatca aagagccaaa gactacgagt ataaagccta
     5041 gagcagaact tttacccggt aatcctcctg tattcattcc ccaaatccct gcgaacaacg
     5101 ccggaacgga aatgattaat gcagctgaat ctagatattt catgatatga tttagattat
     5161 tgtccatcat cccgttgaat agatcaccaa tactctctaa taaatctcga tagatctcga
     5221 tcattttttc tgtttgtcgc tgtctcaaat ggacatcata gagtagctgt tcatcgtcta
     5281 aacgtttgat gaaagtttca gaattccata attgatccat tgtttcccgt tgatctctta
     5341 aggtatgatc gatatatacg atgtctcttt caagatctgc ctgtttatac agttcttcat
     5401 tttccgttgt tttacgagct gctttatcca aagcgtcaat tcgctttttc atctctctta
     5461 gttctttcac gtaatgggtg taaatatcta ataatgtata cgtgatgatc ttttcaaacg
     5521 agtttaaatg atttccatgc tttgtcagca gccgatccac aaattcggtt tttctatcga
     5581 tacaagtaat caagagatta tcggaaagta caaaagatat tggcgttaat tttttttcga
     5641 tacgattatc tgactgatct agattaatca ctaccaaaga aaatggatgc tctagtttcg
     5701 tctcataaag gcgctccatc cgagaaactt cttccggata ttcggttcct acgaaaatat
     5761 catcaggaag ctcgaattct tcgattgctt gctcaatctc ttcgggcttt gcactatcaa
     5821 gtacgagcca attatatttt ttttcatctg tttctattac attcttttct tcatccacaa
     5881 agtagtatct caatgtatac ctcctttttg gactttctcg aaactattag ctgaagaagc
     5941 agttctttaa tttgatatta taatcgagta ctgcgatttc attttctaat gaaaagctgt
     6001 caagctgttt gttgaagtcc atgttcaatc gtttaccgaa ataattttgc tctaatttag
     6061 atgtatagat gtccaataat ggatgctcga ttttagtcgc atattctcca aattccgaaa
     6121 caaaaggaat gatttgaaaa aagctgcctt ttgcaaaagc acctttgtcc cttagaaatg
     6181 gatcggaatc attgataagc gcgagtgcga tttttgtatt ttccccaacc ttttcaatta
     6241 acgtctctac tgcattttta gacattgtaa tttccattcc tactccccct cttcagttgt
     6301 tgtcttctat tatttaacaa ctacatctgt ttttggttga acttttgcat attttggata
     6361 tttagggaat ttagctacac aacggaaagc cgtcatccct gcatattttt cgtaatattt
     6421 cttcgttggc ccttcaaaat ccttgctcca cggtttcaca tgtcctgaaa aatggataat
     6481 gtaaggattg ttgcgagttt cttcgtattc acgttcacct gctgctgttg gatgtttttt
     6541 tgcttttgcc atgatatagc cttgtgcatt ccaacgcgga tgaagtggta accaacggtc
     6601 atgtaagata gcatttaatg catcttggtc atggaatcgt aatttttctg gattatgttc
     6661 gatgaaatct aatacttttt gcgtaatatt ttcatctaac cattttttca cattgatcag
     6721 catcaaacct gagttgaaat aacgcattga tttagctgaa attttcattt tttccagtcg
     6781 ttgatggaac ccagcgtctt caacagctgc tacgattgaa tcaccgaagt caagacgcca
     6841 taatttagag atatcttgta aagcgatcat gtcgcaatcc atatataaga tacgttcaac
     6901 ttctgtgccg cggaacaatt ccggaatcgc aatacgataa taagctgttt ctggaatacg
     6961 atcgctgatt accacatttg taaagaattc tttattgatt ttcaagaatt ccacatctga
     7021 gttcattcgt gcgtttttca ctgagaagcg taaaagctgt ttactttcaa aatcgatatc
     7081 atcgtcaatc acatagaacc gaacaaattt agacgggttg caattatcta atactgagac
     7141 aaataatgca gctaaatgtg gtacgaattt tgtgttacaa cttgcgacta ctgctatttc
     7201 ttttttattc attgatctcg cctactttcg ttagagtact gtgtgctaat ttttttaagt
     7261 ataaattggt atacggatga tctttcaacg tattccaagg tttatcatgt cctgtaaagt
     7321 gaacgatcga aggtttttca tttccttctt tatactgacg ttcatatttt tcatttggtg
     7381 ctggatgacg ttcaaaaatc agcgaagtct gcatgttcca ttttggatga agttgttccc
     7441 aatcttcata aagtacagca ttcaaagcat cttggtcgtg atagatgatt cgatcgccat
     7501 tttcactgag gtaatggata gttttttctg taatttcttt tttattccat tgatcaatgt
     7561 caatcaccat cactcctgaa ttgaagtaca agtcatcgga atcgattcct aatctttcca
     7621 aagctttcac ttggcctgga tcgataatag cgccgattgt ttttccattt aacggctcat
     7681 catatagttt tacgatatcg tctagtacta acacatctga atctaagtaa aggactttct
     7741 tataatcctc tttagctaat aagtttggta aagaaattct taagtaagcg gtagttgtga
     7801 tatggtcact gactaggaag tcttcgtaga tatctttttc cactgtcagg aattggatac
     7861 ttgcattact tgaatattta tttaccgttt cttccaatcc ttctttacta tattctgata
     7921 gaccatcgtc aatcacataa aattttatgc gtcttgtttt attgcaattt tctaatgcag
     7981 ttgcgatcat cacgctcaag taaggagcat aattttcgtc tgaagcagtt accacaggaa
     8041 ctacgccgta tctcttctcc atcaaaatca ctcctttttc taatcttttt ctaacctgtt
     8101 ctacatctag aatattcctc atttggtttt aaagcaaaat atatgctttg ttttcattga
     8161 atattttaaa atttttttat cacaaaacgc atcggtaagc gctaactatt cgtttttcat
     8221 ggctttttat tcaaaggtat tgacc
//
LOCUS       DANKHL010000131         8121 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000131 DANKHL010000000
VERSION     DANKHL010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.135"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            196..897
                     /locus_tag="PW101_002046"
     CDS             196..897
                     /locus_tag="PW101_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2514439.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1506..2522)
                     /gene="tsaD"
                     /locus_tag="PW101_002047"
     CDS             complement(1506..2522)
                     /gene="tsaD"
                     /locus_tag="PW101_002047"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDL2514440.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2519..2983)
                     /gene="rimI"
                     /locus_tag="PW101_002048"
     CDS             complement(2519..2983)
                     /gene="rimI"
                     /locus_tag="PW101_002048"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL2514441.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2990..3532)
                     /gene="rimI"
                     /locus_tag="PW101_002049"
     CDS             complement(2990..3532)
                     /gene="rimI"
                     /locus_tag="PW101_002049"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL2514442.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3516..4238)
                     /gene="tsaB"
                     /locus_tag="PW101_002050"
     CDS             complement(3516..4238)
                     /gene="tsaB"
                     /locus_tag="PW101_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDL2514443.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4429..5409)
                     /locus_tag="PW101_002051"
     CDS             complement(4429..5409)
                     /locus_tag="PW101_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2514444.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5433..6917)
                     /gene="galT"
                     /locus_tag="PW101_002052"
     CDS             complement(5433..6917)
                     /gene="galT"
                     /locus_tag="PW101_002052"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HDL2514445.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6929..7918)
                     /gene="galE"
                     /locus_tag="PW101_002053"
     CDS             complement(6929..7918)
                     /gene="galE"
                     /locus_tag="PW101_002053"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDL2514446.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2301 a         1726 c         1419 g         2675 t
ORIGIN      
        1 attattgttg ttttttatac acaaatcatt cgcttcaaaa caaattattc aataccattg
       61 tatatttttt acataataac aaattatatt tgtaaagtat taaaacttgt ttctaatgtt
      121 ggtatgttaa aactttacaa attctttaaa aaaagtgaca ataaatatag tgaacaataa
      181 ggagaggact ttttaatgcg tgttaataaa atcgattttc acttttcaaa actcagatat
      241 caacctgatt ttgaacattt ctcggatgaa gagttccaac agatcaagga acatactgtt
      301 cttcgttctt ataaaaaagg acagatcctt ttcgacgatg gtgatcccag acaacggttg
      361 tatatcttga ccaaaggact cgttcgttta gaacgttatg atgaaagtgc aacttattac
      421 tactatgact atgtgaatac aaataacctt tttcctatgt caggaatgtt tggagaagag
      481 acctacactt atacagctca agcgatgact gatattgaaa cattctacct tcctgtttcc
      541 tactatgaaa aattagcacg aaaaaatccc gaacagttga tctatatgat acaaaaaatt
      601 tctaaagtag ttgaaataca tgaattgcgt atccaaatcg gattgacctc tagcgccttt
      661 gaccgggtaa aacaaaattt attcattttg aaagaagaat tgggcgtacc aacaaaagat
      721 ggtcatatca ccattcctta tccgatcacc ttaaaagaat tagcagtgaa tagcggcacg
      781 acacgtgaaa ctgctggaca agtaatcaaa caactacgtg attccgggct gttagattat
      841 gacaaaaagc gttttacttt ctgtaagaca gctactactc cagctatgag agaataacgc
      901 gaacaaaaat aagcagtgac cctgataaat atcagggtca ctgcttattt tcctccctaa
      961 aacttctgaa agatccggca gttttagatc actggacaag actcaaagat tttctatgtg
     1021 tgtttcagtt atgtaatatt cactgtttgc aaaaaatact gttagattaa ctagatgaag
     1081 cgtcttgtcc tcatccgatc tagattattc gatattcctc gacaataaac gattactcag
     1141 tgagggcaat cagaggaacg gataatgata aataagctgt aaaaaatagc aaaataacaa
     1201 taatgattaa aagtatgtaa aaaatagggc tttgacaaaa acgtgtcaca gccctatttc
     1261 gattaaacgg tgtgtacctg tcaactcttc ggtcacaagt cataatgttc ataaacatga
     1321 ggagctgttt tttgaaattg ttccttgtga atcagagtta aacgacaggc ttacgaactc
     1381 ttgattaaac ggcgttcaaa agcttgcata cgcggtaata agaacagcca ggtcaaaaac
     1441 atgagaagcc gtttttcctg ggctgttctt attacttgat gcagggcagc tttttcacaa
     1501 cctctttatt cagataaaac caagcttgga tcagcattca actgatagct agcaaaattt
     1561 cttttttgca tttccacatg tgctgctgct ccaatcattg cagcattatc tccacaaagt
     1621 cgaagagggg gaatcactag ttctacctca gggagtttag ctgccaaagc atcctgcaat
     1681 ccttcacgca acccttgatt ggccgctaca cctccagcta taatcagttg cttgactgga
     1741 taattttgac aagctcgaag tgttttagta atcaatacat cgatcacact tgcttgaaag
     1801 ctagctgcta aatcattttt gtctagatct tctccacgct gttgtgcatt atgcaccaaa
     1861 ttgataaatg cactttttaa tccgctaaaa ctgaaatcat aattatcttc atggatcatc
     1921 gctcgcggaa aatggtaatt atcttttccc tgatgagcta gctgatcgat ttcttttccg
     1981 cttgggtaag aaagacctaa tactcttcca actttgtcat aagcttctcc tgctgcgtca
     2041 tctcttgtct ccccaatgat ttcatatgat ccatcttctt gcatatagac taattctgta
     2101 tgtcctccgc taacaagaag tgccatcaat ggaaactgga atggtttaac cagacgggct
     2161 gcatagatat gccctgccat atggtttaca ggaataagcg gcaattggtg tgcccaagca
     2221 aaagccttag ctgcagaaat accaatcagc aacgagccga caagtcctgg tccataagtg
     2281 acagcgactg ccgataaatc ttctgccgac acaccagctt ctaccagtgc atcttccaag
     2341 cataaggtga tttgttctac gtgatggcgg ctagctactt caggtactac gccaccaaaa
     2401 cgtttatgac tgtttatttg agaagcaacg atgttagata agatctctgt tccatttcta
     2461 acgaccgcta cactcgtttc atcacaactt gattcaatcg ccagaatcaa ttcttcattc
     2521 atttcatcgt ctccgttttt aactttctgc tcataatgat cgcatcttcc gtcggattgc
     2581 gataatatga tttccgttta cctaatatac gaaacttttc tgattcataa agttttcttg
     2641 ctgcttcatt agatagacgt acttctaaaa aaacatgcag cgaatctttt atttgttccg
     2701 taatcagtaa ttgtaacaaa tgccgtgcat gcccttttcc ttgttctgaa actgcaacag
     2761 caatatttgt gatctcgact tcatctatta ctttcgagta tccaataaag ccacagatct
     2821 ggttatcttc agctaaaagc agataatcag agtgaggctg ttggatgtct gccaaaaact
     2881 gcattttcgt ccaaggtgaa ccatgtggat aagcattttc gctaatcatc catacgcatt
     2941 ctgccaagta cgcttcatca aaatcttttt ttctgcaaat caactaatct cactcatttt
     3001 ctaaaagttt tatcatatcc aacgcatctt cttttgtttc agtgtaatat ctttttttga
     3061 tcgttgttgc ttgaaacccc aatttccgat aaagtctttg tgcatcttga ttactcatac
     3121 gcacttctaa tgacatcgtc tcacagcgat tcattatagc aaagttctcg atttcgtcaa
     3181 taagcagcga accgattcgt ttacgctgat atgccggtcg aacggcaata ttcgtgatat
     3241 ggcaatctaa accgaacgct cttgaaccga taaaaccaat cattcttcca ttatcaaaga
     3301 tacctagata tagatgagga agcggtgact tcaattctga tataaaggca ctttttgtcc
     3361 atggcaattc gccattatag acttctcgtt cgacatccag cagctctttg atatcttctg
     3421 tagtcaattc tcttagtacg taacgacgat cgatttgttc gactgttttt tctgtataaa
     3481 tcattttatc ttgaaaaaag cttctggcat acgctctaaa ttttttcaac ataactttcg
     3541 tctccaggtg tgtgcgtctc caaccatttt tcttctgctt ctactcgttt taaatagcga
     3601 ggaagaaaag cttgtttctc tgcaactggt tcaactaaag cacctaattg agccacagtt
     3661 acaccacttg gaatatccca tgctggaact gtattaatca tcgcactagg caatatttcc
     3721 tttatttcag agaagaattt gtgacaatca ctcccgacga aataaatagc ttcattcagc
     3781 ttttgtagtt gtgtgaacaa atcagaagca gaaatatgta catcttcaag tacagtttct
     3841 aatctatttt ctttccagcg gtatgcccca gcgtatacat tttttcttcg agcatcaaac
     3901 atgggcacaa taatattatc cacaccgatg caattagccg cgatggtttt cagactagat
     3961 actcctacta gctctttatc taatgtataa gcaatcgttt ttgctgtcgt tacacctata
     4021 cgaagccctg tataagaacc gggaccatca gaaaccgcga ttcggtcaat gtcttttgga
     4081 cttatttgaa gatctgcaaa caattggtcg attgcaggca tcagtgttac actgtggttg
     4141 cgtttgatat ttgtttgtat ttgtccaaga atttgttcgt cttctaccac accgatcgcc
     4201 aaagtttggt ttgcagtatc tattcctaat gtaatcatat atttgatcct ttctgctatg
     4261 acattctcgc cttccattgt agcatacttt tttattctga ggatgaaaat accaaaggca
     4321 aaactagtac aataatacat gattatctgc tggcgggcaa attttgcccc ttcctgaaac
     4381 acaaaaaagc tgcggtatca ctccattttc ccacagcttt tccatctttt atttcgttga
     4441 ttctcgaata atcaactctg tttccacaat aatctttctt gaaaccggtg ctttggaatt
     4501 acttaattga agaatcgtct ccactgctag ctctcccatc cattcggtat aaacttttac
     4561 agtagaaagc gagggcgtta catatttcgc tacactgaca tcattgaagc ccatcacaga
     4621 aatatcttct ggtaccacta aacctttttc ttgaatcgct ttcagtgcgc caatcgctaa
     4681 tgcatcatta gctgcaaaaa gcgcatcagg aacatgcgga ttcgttttta aatattgctt
     4741 cactgcttga tatcctgact cgacagaaaa atctgcttcg ataattggac cttcgtcgta
     4801 gcctttttct gccaaaactt gttgaaatgc cataattctg ggatctttta gccgctgatg
     4861 gtttttcttc gtatattctg ttccagcaag catagcgata tgttgcgctt tatgggagac
     4921 aaagtgttca atgacttttt gaacacttga cttaaagtcg acaactagag agtcgattcc
     4981 ttgagaagtg ccgtcagaat ctaccaaaag caaggcattt tgggatcctt tcatttctaa
     5041 taactgagct tcgtcgaatt ttcctagtgc gatcgttcca tctgccggaa gatcactaat
     5101 ggaatcccac gtttctttca ataaagtaac gtttgcttct tctgcctttt tttcgattcc
     5161 taggcgaata gataaataat aaagatcttc aagctcttcc gtatcgtcgt accactggat
     5221 caggcgaagc gtttgcttag tatttgctgc atttttacga tgtttcgtat aatttaagcg
     5281 ttctgctgct tcaaaaattt tttgctttgt ggctaagcca acggaaagat caggatcata
     5341 gttgagaact cttgaaacag tagcaggaga aactcctgcc aactgcgcaa tgtctttaat
     5401 tgttgccatt tgtctctcct ccttttttac gtttataaaa tcgcagtaaa tcgttcaaac
     5461 gctgcctgtc ctttttcatc tcgcttgaat actccagcgt cttccagtac acgtgcaaaa
     5521 acctgaccta cagatttttg tacgacatcg gtgaccgtat ctttcgtcaa atcaggatgt
     5581 tcttttttta attgttctgc ccacggacga tgatattcag ccatctggtt ttcttctcct
     5641 aataagaaat gctctacttc tactagttct ggtttcaaac gaggtggtaa aacagcaagc
     5701 cccattactt cgatcaaccc aatgttctcc tttttgatat gttggacatc tttatgtgga
     5761 tgaaaaatcc catcaggatg ttcttctgaa acattgttgt ctcgaaggac aaggtccaac
     5821 tcaaagtaat tgtcccttct tcgtgcaatt ggcgtaatcg tatggtgtgg tgtaccatct
     5881 gctgaaaaag ctctgataga tactgtttca tccgaatatg ctttccattt ttccaatact
     5941 gagttagctg cttcaataag caagtttttc tctgttgatt gcaaacggat cacagacatc
     6001 ggccatttca cgactcctgc gatgacttgc ggataatctt ctagtttgat aagtttttct
     6061 atcggtgctt ttgccatagg aaattcatga cgtcctgcct gataatgatc atgagaaaga
     6121 atcgatcctc caacaattgg taagtcagca ttcgaaccaa caaagtaatg aggcaagact
     6181 tcagtaatcc gaagcagtct agcaaatgtt tcctttgtga ttttcatcgg gcggtgttgt
     6241 tctgataaaa tgatcgaatg ttcattgtaa taagcataag gggaatattg gaatccccag
     6301 ctttcaccgt ctagattcat tcgaataatc cggtgattgg tgcgggctgg atagttcaag
     6361 cgacctctat atccttcgtt ttccatgcaa agcatgcagg taggatagtc tacttttacc
     6421 gcctcgcgtt ctgctgcgat ttgttttgga tctttttctg gcttagataa attgattgtg
     6481 atttccagtt caccatactc cgactctgcc ggaaatttga tgttttttgc gatggctctt
     6541 gttttgatgt agtcattttt cttacttaaa ttaaagaaat actcagttgc ttcctctggg
     6601 cttttcgaat agtgctgtgc aaagaatgca ttgacgactg aaggtggcgg tgtgagaaaa
     6661 tccattaatt gcgcttcaaa aatctcttgt tctgctggca attctgaaag aacaccattt
     6721 tctcttgcct tagcgacaag ctgatccaat aagtctacag attcttcctt aactggtctt
     6781 acttcaatgc tttctagagc atcttctcct accatcccta aaagacgatt ctgtaaatat
     6841 aagcggtcca tttccatcca tccgccagct tcgatcgcaa gtgtggcaaa atcacaaatc
     6901 gtttgactga ttgacatatc tcattcccct attcttcata accatttgga tggctgacat
     6961 gccaatccca tgctgttttg ataatcgctt tgatatctgt gtattcaggt ttccagccca
     7021 gaatctctct cgctttttca cttgaagcga ctaatcggct aggatctcca gctcttcttg
     7081 gtgctatttc tgctggaatt tcttttcctg tcacttctct agctgcttct agcatttcct
     7141 tgactgagta tcctttgctg ctgcctaaat tgaagaagtt gctttcattt ccttctttca
     7201 agtattccaa agctaaaata tgtgcagcaa tcaaatcttc tacttgaaca taatctctga
     7261 tacatgttcc atctggtgta tcataatcgt ctccataaac agccaaggct tttctttgac
     7321 caagagctac ttgtaaaatg attgggacaa gatgcgtttc tggtgtatga tcttctccga
     7381 tagatgcatc tgcttttgct ccggctacat tgaagtagcg caaagcgaca tagcgcatgc
     7441 catatgcttg gtcacaccac ttcatcattt tttccatcat caatttactt tcaccatacg
     7501 gatttttagg attagctggt gtatcttctg tgattggaga ctctttcggc tctccataag
     7561 ttgctgcagt agaagagaat acgattttat tcacgttgaa ctcgtgcatg acttcaagta
     7621 agatttgcat accgtatacg ttgttgttga aatacatcaa tggtttttcg acagattctc
     7681 ctactaaaga gcttgcagca aaatggatta ctccttcaat cggttccttt tcaaatacgg
     7741 aacgtagaaa ttctttatcc cggatgtctc cttcataaaa atgagcatct ggatgtactg
     7801 cttgtttgtg acctgtgagc aaattatcta ccacagctac ttgatacccc ttttgaacta
     7861 gctggtctac agcgtgcgaa ccgatataac cggctccgcc tagtactaaa attgtcataa
     7921 aaaacactcc tttgattagt aaaattgtgt ttgttgattc tatataattt aaaaaacgaa
     7981 gtttactttt atggtgaaaa caaacaaagt aaaatttcgt aaattcatat ggtaaattta
     8041 taataaaaca tttagtaaac attttcaagg gttttttgca aaggtttaca aacaaacgta
     8101 aaactagcct ttttttctct t
//
LOCUS       DANKHL010000132         7986 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000132 DANKHL010000000
VERSION     DANKHL010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.136"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(4..852)
                     /locus_tag="PW101_002054"
     CDS             complement(4..852)
                     /locus_tag="PW101_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2514447.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1076..3016)
                     /gene="thrS"
                     /locus_tag="PW101_002055"
     CDS             complement(1076..3016)
                     /gene="thrS"
                     /locus_tag="PW101_002055"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDL2514448.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3353..3532)
                     /locus_tag="PW101_002056"
     CDS             complement(3353..3532)
                     /locus_tag="PW101_002056"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDL2514449.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3579..5402)
                     /gene="cadA"
                     /locus_tag="PW101_002057"
     CDS             complement(3579..5402)
                     /gene="cadA"
                     /locus_tag="PW101_002057"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL2514450.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5680..7959
                     /locus_tag="PW101_002058"
     CDS             5680..7959
                     /locus_tag="PW101_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2514451.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         2335 a         1704 c         1447 g         2500 t
ORIGIN      
        1 tttttatttc gaatccataa aatattcttg gattgcttct cgtgtttttt tcactttttc
       61 gctaagcttt tcgtttttag ccatatagaa ataaaaaatg atatcgatcg tagcaaattg
      121 ggccaaaagt gaattagttg ccgcacttcg attcgtgatt tcttttggtc tagatgtttg
      181 tacgagtaca tcggctatct tgctaagcgg gttatttcca aattgcgtca acgcaatgat
      241 ttcgatattc atgcttttcg ccagctcggc taatgcgact acatctgcac ttctacctga
      301 gtttgaaaca agccaaagca ctttcttttt ctcattgctg acaagttgcg ggagcaagac
      361 gtggatatct ttttcaaaaa tgattggctt accgacacga gaccattttt gcagaatatc
      421 ttctactact aaagaagagg cgccaacccc atataaaaaa acagtctctg ccgattccaa
      481 acactctacg actttttgga tcgtttcttc ttctagaata tctactgttt cttttatcgc
      541 tacttgtgca ttgttaccaa gtttgttttt gatagagtaa aaggactcat ttgcttctat
      601 atcactaaaa ccttcatcga ttccagtctg tagttctgtt gaaatcgtga ttttgaagtc
      661 cgtcagactt tgaaaaccga tttttttgga aaaacgtaca actgtcggcg cactcaaccc
      721 ggaagcttct gctaattcat tagctgtcat tgtagggacc ttctcaatat taccgataat
      781 gtagttggcc aacttttttt ctgctgaaga ataatcttcc atcatacttt caatacgact
      841 aattacgctc ataagttcct ccaccctttt aatgatagtc tatgtaagta taccatatca
      901 gacccttatt cacggtcgta tactggataa aaaacaaaaa aacaatccgc ccaacagaat
      961 atcttatccc atcaatccca cagattctat tgagcatctg tgtctttgag gaatacctgt
     1021 tatctattgt tcggattgct ttctatctac tactttttta caggaaattt acctattagt
     1081 tttctctgct gtaatttttg acttctgctt ccatagcatc aataaagata ttcaaatctt
     1141 ctacagctgt ttctttactt ccataacgac gtacgttcac tgttgcatct tccaattcct
     1201 tgtctccaac aactaattgg taagggatct tttgtgtttg agaggcacgt atcttatagc
     1261 ccattttttc attacgatca tcaacttcta cacgcaaccc cttcatttgc aaacgttctt
     1321 tgatttcata agcataatcg ccatgtgcat caacagaaac agggatgatt gttgcttgta
     1381 taggtgccaa ccatgttgga aaagcgcctt tgtatacttc tgttaaataa gcgacaaaac
     1441 gttccattgt agaaacaatt ccgcggtgga taactactgg acgatgcgta ttttccccgt
     1501 cttctccaac atatgtcaaa tcaaagcgct ctggcaataa gaagtctaat tgtatagttg
     1561 ataatgtttc ttctgtacca agagctgttt tgacttgaac atctagtttt ggtccgtaaa
     1621 aggctgcctc gccttctgct tcaaagtaat ctagttctaa ttcatccata gcggctttca
     1681 gcattgcttg ggctttttcc cacattgcgt catcatcaaa atatttatct gtattgtttg
     1741 gatcgcgata gctcaagcgg aaacgataat ctgtaatatt gaagtcagca tatactgcca
     1801 ccatcaattc aagcgtacgt ttaaattcat ctttgatttg atcaggacga acaaatgtat
     1861 gaccatcatt caacgtcatt tctcgtacac gttgtaaacc tgacaaagca cctgattttt
     1921 catagcgatg catcattcct aattcagcga tacgaattgg taattcacga taagaatgga
     1981 tcgtgttttt gtagaccatc atgtgatgag ggcagttcat tgggcgaaga acaagcattt
     2041 cgccatctcc catatccatt ggcgggaaca tgtcttcttg ataatgatcc cagtgtccag
     2101 atgttttgta caactcaaca tctcccatta ttggtgtata tacatgttgg taacctagac
     2161 taacttcttt gtctacgata taacgttcga tcgtgcgtcg gattgttgcc ccttttggaa
     2221 gccagaatgg caaaccagaa cctacttctg gggaaaccat gaacaagtca agttcttttc
     2281 ctagtttacg atggtctctt tcttttgctt cttcacggag acggatatat tctttcaaag
     2341 ctttcttgtc aaaaaaggct gtcccgtata cacgttgcat catttgattg tttgagtttc
     2401 cacgccaata agcacctgcc actgataata atttaaatac ttgtatacgg ccagtggatg
     2461 gaacatgtgg tccacgacac aaatcgatga attcgccttg ttgataagca gtaatcactt
     2521 cttcttctgg caattcacta atcagttcta ctttgtacgg atcacttgca aaaatttcta
     2581 atgcttcctg ttttgaaaga acacggcgtt cgatcggcag attttctttg acgattttca
     2641 tcatttctgc ttcgattgcc ggcaaatctt ctgcagttac ttggttttgt ccattatctg
     2701 tatcgtagta aaaccctgaa tcgatagctg gtccaacgcc aaagtggata tttggataga
     2761 ggcgacgcat ggcttgggcc atcaaatgag ctgctgaatg acggactaag ccaagtgcat
     2821 cttcatggtc tggtgtgatg atctcaatac ttgcatcttc ctcgattgga cgagtcaaat
     2881 caacgagttt tccgttcact ttaccagcta aagctttttt tgctaagctt tttgaaatgc
     2941 tttcagcaat tgccaatgtt gttgttccag actcaaattc tttgactgca ccatctggaa
     3001 aagtaatttt tatcataata gtttcctcct tgaaatgttt tgattgatca gatcatcaag
     3061 ctgaaaaaca aaaaaatcct agcatctctt gaaaagagat actaggacga aaagttcgtg
     3121 gttccaccta atgttcaaac gcaaatgcgt tcctcaagcg ttgtaacggt ccgaccgccc
     3181 ttgcttcaac aagggtttcg aaagtggtca tcctttggtt ttctcctgga aaagtttcag
     3241 cctagctttt cctctcttat cgaagactct caaattcagt tggctttctc atcaatcaat
     3301 tatgtgttta tttaaccact aataaataga aaaatcaagt gggaaactaa acttattttt
     3361 tccattcccc atttacaccg tatgttcctt ttttcatctc attgatgatg acatggatgt
     3421 gttcttttgg tgcctgcgtg tttttatgaa cagcttccgt gatatctttc atcatatttt
     3481 ctaattgttc cggactgcga ccttctacta attcgacatg tacgaatggc ataatgctct
     3541 ctcctctttc tgatcgttct tttttcaact cgattctatc atactatgtt tgtcttgggt
     3601 actgaatcag gaaaaatggc tggatctttt tttgctctca aggcactcaa aatagagacc
     3661 gtatccacga cttcttgcaa aacagctccg aataatgcgg ggattagtcc cgtacttgca
     3721 attaacatca aaaaaataca aataaagatg ccaatcagta cagactgttt cgctattctc
     3781 atcgtatctc ttgaaatcac tactgctttt gctacacgca agagatcatc tttcaagatg
     3841 accgcatctg cactttcact agcagctgta gaaccatgag cccccattgc aataccgata
     3901 tctgcagcag caagtgctgg tgcgtcattc acgccgtctc ctaccatcgc aacaggtctt
     3961 tgatctttag ggatactttc taaaatggtc agtttatctt gaggcaagct acgggcatgt
     4021 acagaatcta attggacttt ttctgcaatc gtttgagcaa tggcagatcc atccccggtg
     4081 atcatcatca aatttttcac atcaagtttt cttagctcct tgattgtctt tgctgcttct
     4141 gggcgaatct gatccgaaaa taggatcgtt cctgcaaaag atcgatcaat tgttacatag
     4201 attgctgtat tggacgtttc tatttcttca gctgaaacaa aagaggcacg tccaatcttt
     4261 acttcatgat tattgactac tgcttggatt ccttgaccgg tcacttcttt cagttgacta
     4321 acaggataca gttggctgtt tttcacgttg ttcactaacg aacgagctaa gatatggcta
     4381 gattcctgtt ctgcactagc tgcatacaga agtagctctt ctttttttat tccattgact
     4441 ggctgaatcg cctctacttc caatatccct ttggtaagcg ttcctgtttt atcaaaagca
     4501 atcgtttttg tacgtgcgag cttttcaatc gttgttccag tttttacaac gatcccgttt
     4561 ttacttgaac ggctcattcc tgcaactaaa gcaatgggcg cagctagaat cagtggacaa
     4621 ggtgatgcca ctaccagtac ctcagcaaat cgtaaagggt cttttgagaa ccaccaagaa
     4681 ataccaccga tcaaataagc aatcaacgtg aaagggactg cataacgatc agctaaacgg
     4741 acaaatcggg caggcttttc ttttgattct tcgactagtt taataatcat ttgatactgg
     4801 ctgtctgcgg cacgtttttc agcgatcatt tggatcgatc cgtctccatt taaagaccct
     4861 gacataatct gatctccagg tttcttggtt tccgggcggg actcacctgt caaagaagat
     4921 tcatcgaccg aagattctcc tttggtcact cgaccatctg cgggtacaag ttcgccaggc
     4981 ttgactagta actggtcatc aatatttatt tcatcgatta ctatatcctg caattttcct
     5041 tctttgaatc gatgggcaat tcttggggtg ttatctaaca aagactgcag ttctttaccc
     5101 gctcgtttat tcgcgtaatc ctctagacta tctccgcccg taagcatgat caatatcatc
     5161 aaactagccc agtattcttt gacggctaat gttgcaagga ttgcggtaat cgctaataca
     5221 tctacgccat atcttcctga acgtaacgtc ttgatcattt ccaaaaacat caagaaggcg
     5281 agaatccctc cagtaaatgc gacaatcgca aatgcaaggg ccggctggtg aaaaacaaat
     5341 tcacttaata aagctatccc accaataagc aaagtaagta aaaatttttt gaaatgtgtc
     5401 attgtttttt ctcctctttt atgtgactta gagaggttag taataaattt ccagctgcct
     5461 ctttcatctt aagcatagtc cttgactagc actttttaaa acaataggcc ttaattgaaa
     5521 acaactatca ctatcagcgt ttccaaaatt catcagttct acaaacaaaa atattcagca
     5581 aggattactt tttcgattca tcagttaaaa agtacctttt ttcgaaaaac tatgctatac
     5641 ttttaattcg ttatttacgg cggaaggagc aaacatgcga tgaatgagaa acaactagaa
     5701 caaacgcacg tggatgaaac gatcgaacta atccagatcg aacaaaatat gctggataca
     5761 aaacaacaag aattgactca ggaaatgaat cattcgacaa aagacacagc taatcacaag
     5821 attcgagctg gttccaatga agctttttat gaatctgctg ttgaatatcg ccaacatgag
     5881 caggaattgc ttttgaaata ccatactctt gaagcacagc aaaaacgcct gcaaacgttg
     5941 tctgtcatga aagatagtcc ttattttgcc cgtatcgact ttaaagaaga ccgtgaaaca
     6001 gaaacattat atctaggtat cgcttcttta agagatctgg cagaagaaac aatcgttatt
     6061 gactggcgcg caccaattgc caatctttat tatgaaggtg agattggtcc cgcattttat
     6121 gaaactgaaa cagatagaat agatgtcgat ctgcagttga aacgacaatt taaaatccaa
     6181 gaaggacgta ttttatctat ggtagatacg tcggaagtaa tcaatgatga ctttttgcta
     6241 gagatattgg atgaatcatc aagcaaccat atgaaaaaca tcgtctctac gatacaaaaa
     6301 gcacaaaatg caatcattcg ggacacaaca agtaaagtaa tgctgattga aggaatcgcc
     6361 ggaagtggaa agacatccgc tttgcttcaa cgtattgctt ttctgttgta tcataatcgc
     6421 aagtggctcg aggctgaaaa cgttcttcta ttttcaccca accatttgtt ttctgattac
     6481 atctcgactg tattgccttc attaggtgaa agcggcgtgc ctacacagac attcaaaaat
     6541 tatttggtgc agctgctgcc agagttttct ttactagatg aacagcaaca agaaagtggt
     6601 tttttgctag gggaaaaaga tccgatacaa gtgatgaaaa gcggattgac cttagtcgat
     6661 cagattgatc gctatatcca aggcatcaca aactacggtc ccttgttccg tgatatgaaa
     6721 ataaatggcc gtacgatcct atcaaaggaa tcgattcgcc aatggtataa agaaacaaat
     6781 gaacaattac ctttacatca tagactttct ctattacaaa cgaagctcct gaaaaaatta
     6841 ggcggtctgc aaaaagacga aacacgtcag caatgggtca aagatctggc ggaagaacag
     6901 ctccaagaac tatacgcaac agatccaaat cttgaatata cagaacaaaa ggagcgttca
     6961 ttacgtaaaa aactggccaa ccaaatcgta cgtaaaagat tccgtcgaat ccatcgaggg
     7021 atcatgaatt atcaatttgt caatataacg aaacaatatc tccactttct acaaactgtg
     7081 ccactttctc ttctaacacg acataaaatc agcgagacca ggtggcagga acatttgaca
     7141 actgttcgta cacacttgcg tcaaagagaa ttgcgtcaag aggatgctgt actattcttt
     7201 ttactgatgc gccgaacata tccagtgacg gttacacaca aagctcgcta tatctttatt
     7261 gatgaaatgc aggatttcgc accatcacaa gtggcattgc tgcaagcctt gttcccaaaa
     7321 gcaaatttga ctttctgtgg agatctaaat caaaatgtct ttggaaatga aacgatcgtt
     7381 ggatcattgg aagacctttt cgtggaccgt gaagtcactc gtttccaatt aacgacaagc
     7441 tatcgctcca caaaagaaat cactgatttt gctaatcatt ttattgtgaa agaacatcaa
     7501 gtagaaacaa cagcaagaaa aggagacctt cctttgctca tccaaggaac aacagatgat
     7561 atcaaattga attggttagc agataccatt cgaacgactc agggaaaagc ccggtattgg
     7621 cgtacagcaa tcatagctaa aacacaagaa gaatgcgaag ctctttatgc tcaactgcca
     7681 gacgagttga aacaacaagt ccagctgatt ttaaatgaga gtgactttat gaagcgttcc
     7741 attattttac ttccagcctt tttagcaaaa ggtctggagt ttgaccgagt tttcttgtgg
     7801 gatgtagatg cagaacattt ttcttctcag caagacaaat tgatccttta caccatgtgt
     7861 acacgtgcga tgcatgaatt gacccttttt acctccaata atgaaagtcc tttgttaaac
     7921 aatatggata tttcatctta tgagaaaaaa gtactgtaag taaaaagaca actgctatca
     7981 ttaatg
//
LOCUS       DANKHL010000133         7979 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000133 DANKHL010000000
VERSION     DANKHL010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.137"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="PW101_002059"
     CDS             complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="PW101_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL2514452.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1228..2331
                     /locus_tag="PW101_002060"
     CDS             1228..2331
                     /locus_tag="PW101_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2514453.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2524..3591)
                     /gene="rlmN"
                     /locus_tag="PW101_002061"
     CDS             complement(2524..3591)
                     /gene="rlmN"
                     /locus_tag="PW101_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDL2514454.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3821..6268)
                     /locus_tag="PW101_002062"
     CDS             complement(3821..6268)
                     /locus_tag="PW101_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDL2514455.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            complement(6457..7893)
                     /locus_tag="PW101_002063"
     CDS             complement(6457..7893)
                     /locus_tag="PW101_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2514456.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         2213 a         1736 c         1359 g         2671 t
ORIGIN      
        1 ggtacacggt gttccaaaag tagcttcttc ggaaataagt tggaatttca caaaaattca
       61 agaagtaatt ttcgtaaatt ccttcttatt tcttgaagct gattacttct gtcacaacct
      121 ctttttatta ttgagaattt ggtcgaggaa taatcgtttt tgccatccaa atatccggtt
      181 tgtcccagcc atttgcattt ggtaagacac ctacgatttt ataacctaat ttttcataga
      241 attcatacgg atgttcacga aggttctgga tggaagccac tttatcaaat gtatgctcat
      301 acaggtccgt ttgacttaac gttgttccat ggtctaaatc atccgtacct aaataaatcg
      361 tgattcctcc tctggaagct acttcttttt ctaagtaatt gactaatcga gtacctattt
      421 ggttctttcg tcgggagctt tctacaacta atggatgcaa ttcccaacct gtgataccgt
      481 attgagggat tgcaccaata aatcctacta actcatcttg gtctaccgct gctaccgcga
      541 ttcgttctgg attcatcatt tcttctactt cttctgccga gctgtctcca tattcttccg
      601 gccaagtcag tctcagtaaa tcagaaagct gatctttcaa tactggatta ttacggtcaa
      661 attcactgat tatcatacgt cttccccctt gttcttgctt attctaacag tccttttgaa
      721 aaaacgaaat gttctcgctc aaaaatcttt agctaccaat cagtatctct attctttcat
      781 tatttatctt acttataaaa taaaaaatta tacagaaaca ctttattcaa aataggaaag
      841 atcaggaaag ccatcaattg gaggaaaaaa ctctgtttgt tcatcatgat atagattatc
      901 ttttgcttta aaaaaaacca attatgaatt gacaaataaa acctgttttc atataataat
      961 cttaattttt agaattttta gataattaca aaaaagcaaa gggaatcggt agaaatacta
     1021 agaggaacaa aaattcatta ttgtcgtttt caatgttttt gtacttgttc gtttttagga
     1081 tacttattga tgatttcaag gtgttcatgc caccttattc atcacttatc ctgtcttagg
     1141 ttcacctcta ctccatctac agccgcagtc agatcgatca ctctttaaaa ttttaacccg
     1201 tttcaacgaa gaaagaagga aatgaatatg agtcaaagtg ttttcttacc aaattatacc
     1261 attggaacag atgcgtatga aaaagcaaat ggaatcgtca aagaatatgg gacaaaagta
     1321 gtgctgattg gagggaagac tgcactttca aaggcagaaa acaaaataaa agtagctttg
     1381 acacaggaaa tagaggttgt cgccagtctc tggtacgggg gtaattcaac ttatggaaat
     1441 attgaacagt tgatccagca tgaaagtgtc caaaaagcag acgtcctgtt tgcagtcggt
     1501 ggtggccgtg cttgcgatac tgtaaaagtc attggtgaaa aactagctaa accagtcttc
     1561 acctttccca ctattggttc gaactgttcg cccgtcactg cggtatgtgt catctataac
     1621 gaagaaggcg taatggacgg actcttcttt ccttcaaaac cacctgtaca caccttcatt
     1681 gatacagcaa ttattgccga agcacctgat aaatacttat gggcaggtat tggcgatgct
     1741 ttatccaaag aagtcgaatc tactttttct gctcgtggcg atgatctagg ctacacggat
     1801 agattaggaa tccaaatcgg aaaaatgtgc attgatcgtc tcttgactta tggtacgcaa
     1861 gcattgaaag atagtgccga cgatcgtccc tcatatgcaa ttgaacaagt cgtttcggat
     1921 atcattgtta ctaccggatt ggtttctgtt ttagttaaca ataactataa ttctgctatg
     1981 gcacactcat tctattacgg tacgactgtt ctagaaaaaa tggagggata ccttcacgga
     2041 gcatctgtca gttatggtgt actgacctta ttgatgatgg atcagcagtt cgatttgttt
     2101 gataaaatct atgtgtttat gaaaaagaac caacttccag tatcgttaac cgacatgaat
     2161 ctcaatgtca cgaccgattt agaagcaatc ttagataaag ccatggcaac aaatgatatt
     2221 aaacatgtgc cttatgaaat cacccgagaa aaatttaaac aggccattct tgacgtagaa
     2281 acttatcatg ctacacaaga aagccagcaa ggatataaga aaatcagtta aataattgct
     2341 aaaaccccca tcaagcaggc tgtcatcaaa ttgaggacat ttctttgctt gatgagggtt
     2401 tttcttgtca aaaaaagctt tatagctttc gctatagaat cttttctctt atttaagttg
     2461 aacagttcct taattaagaa aactgctcct tttatggtaa ttgaaaaaac gagtaggata
     2521 cgattatttg gcaactgttt ttttcatttg cttgcttcgc aattgtccgc aagctgcatc
     2581 aatatctgta ccatgttctt tacgtatcac acagtttacg ccgtttttct tcaatacatc
     2641 ataaaatctc aatacatcag cttttggact gcgagaatat tgatcatgtt cactgactgg
     2701 attatatgga atcaagttga catatgctaa ttttttctta tcttttaata gatctgctaa
     2761 ttgttgcgca tgctctggtc tatcatttac ttgattcaac atgatatatt caaacgtgat
     2821 tcgacggttt gttttctcta aatactcatc cacagcttcc atcaatttct caattgggaa
     2881 acttcggttg atccgcatga tcgatgttcg cacctcatta ttcggcgcgt gcaaggaaat
     2941 agccagattg acttgcaagc cgttattagc gaattccttg attttgtgtg ctaatccact
     3001 agtggaaact gtaatgtgcc gagcaccaat tgccaatcct ttggcatcat tgatgatatg
     3061 caaaaagtcc attacattgt cgtaattatc aaatggctca ccgatcccca ttacaacgac
     3121 atgtgaaacg cgttcgccaa gattgcgctc atcaaaataa tgttggacca tcatgatctg
     3181 tgcaacaatt tctccagcag tcagatcacg gttctttttg agcagcccgc ttgcacaaaa
     3241 cgtacagccg atattacatc ctacttgtgt cgtcacacaa actgacaagc catattcttg
     3301 tctcatcagt acagtctcaa tcatgttttt atcagggagt tcaaacaaat attttactgt
     3361 accatccgaa gcttcttgga cgaccatctg ttttagagga ttgatgataa aatgctccga
     3421 taatttttcg atcaattgtt tcgatagatt cgtcatttct gtgaaattac ttacacgttt
     3481 ttgatacaac cattcccaaa cttgggcagc tcggaatttt ttctcgccgt tttctataaa
     3541 ccaattgatt aattcttcat tcgtcaagcc ataaatggac tgtttttcca aagtattttc
     3601 cctctttttc tcaaagtttc gctacatact atacatgatt catttcctta atgcaatact
     3661 tcacaaataa atcgaataga actttaatct cacaaaaact aaaaagacaa gacatcatac
     3721 cgtattagaa aagatggaaa aaaaccattt tttcttttgg tatcatcatg ccttgtcttt
     3781 ttattggtta ttcgtttttc aacccttcat cacacaagtt ttacatgtca ttatcgtccg
     3841 gcaacgtttc tgatgtttct tcctgctcaa tgaatacttt ccgaggcttg cttccttctg
     3901 acgggccaac aactccatga gcttccagtt catctactaa acgtgctgct cggttgtaac
     3961 cgatccgaaa tcttctttgg agaagggaaa tacttgccgt ttgcatttca acgacaagtg
     4021 cttttgcttc ttcaaacagc tcatcttgtg gctgttctgg agcacctgtc gtttcaactt
     4081 catctgtcgg catcattttc tcttcataat gagcttcttg ctgatctgta acaaattgaa
     4141 cgacccgttc tacttcgtgg tcagaaatga atgctccttg cacacggatc ggtttattct
     4201 cacccattgg caagaatagc atatctcctc gccctaagag cttttctgcc ccattagaat
     4261 caatgatcgt ccgagaatct gtaccgcttg ataccgcaaa tgccattctg gaaggtacat
     4321 ttgctttgat gattcctgtg ataacatcca cacttggacg ttgagtagct aggatcatat
     4381 gaatccccgc tgcccgagcc atttgcgcta aacgaataat cgcatcttca acttcattgc
     4441 tggctaccat catcaaatca gctaattcat ctacgatcac tacgatgaat ggtaaggttg
     4501 gatgtttcgt tccgttttcc aagtttttct gttgaacaaa ttcattgtaa ccagaaatat
     4561 ttcttactcc ggttgccgcg aatttttcat atcgttcttc catttcttgt actacttttt
     4621 gtaaggcttg cgctgctttt ctagggttcg tcacgaccgg cgtcaacaga tgaggtatgc
     4681 cgttgtacat attcaattca accattttcg gatcgatcat catcagtttt acttcatggg
     4741 gcttagcacg cattaatata cttgtgataa tcccattaat tgctactgac ttcccactac
     4801 cagtcgaacc agccacgagt aagtgaggca tcttcgaaag atcagccgtt tgtacccgtc
     4861 cagaaacatc acgtcctaaa ggtacttcta gtaatttatc tgggtggttt ggttgtgctt
     4921 cgatgatttc tctaaaggaa accatactga tctttccatt tggtacttca ataccaatca
     4981 gtgatttacc tggaatcggt gcttccatcc ggacatcttt cgccgctaaa gcaagcgcga
     5041 tatcatctgt taagttcaca attttactta cttttacccc tacagccggc tggacttcga
     5101 attttgtgac ggatggtcct aagctggctt tgacgacctt ggcatcgaca ccgaaacttt
     5161 ggaaagtttg ttctaatacg ccgatatttt tttcaatttt tttatactca tcactttgat
     5221 ccacagttgg tatagaatca agcaaatcaa ctgtcggtaa ttcatagtct tgattttcct
     5281 gttctgcttc aatctcaaat tctaatgagg taccgtcatc ctctggcaac tcatctgaaa
     5341 aaccttctgc tgtattggtc tgttttggtg tatctggtac tggtttctct agattcgcag
     5401 gatggatatt ttcttgaaaa ctgtcgattg gaacaaaact tagctgctcc ggctctgtat
     5461 tttctggttc ctgccattca tcagcagtat tttccctttg ctggctcacc gcttttttct
     5521 tctcatattc tgcagcttct ttttctgcag cttcttttgc agctaagcgc cttgcttctt
     5581 tcgcttctgc gcgctgtttc attctctctt cctttgccgc ttgctttcga gcttgcttgg
     5641 caggatcacc ttccaataat tcctgtactc gttctccaat tgattggata ccatttaaca
     5701 gctgatagcc ttccagatta ctgaacagga atgctcctcc tgccagtaat agcactgctg
     5761 ctaaataact tccaggctgt gcaatcaaga agtaagtaaa atgataaagg attgctccaa
     5821 tcatcccacc accaacgtta ttgtctactt gactttgttt tatatcactg gctaacaaat
     5881 cccatgtcgt ccccatgata tttggttccc cagtatggag tttgccaaat aaatgggcat
     5941 gtaacaataa taagataccg aaataaaaga gtatgccgcc aaaccagcgt cggtttttac
     6001 caattgaaaa ctctgtattc ttaatgacaa tccaaaatcc taatacggct aataaaagac
     6061 agaggatttg gtaagtattt cctgcaatga tccgaaagcc gtttgcaatc aatattccta
     6121 aaaaacctaa ttttaaaaag ccgaagacag caaataaaat caaggcaaac ccaacaccaa
     6181 tcgtcagcat ttgttcctgc tgctgttttt gctttttcgt ttttttcgtc tttttccttt
     6241 ttttagtagg ccgtttcttt tgtgccattt attatccctc atttcatcaa gtttcattat
     6301 acaaaaaaaa gtaagggaac taaagttttg tttgaaagaa ctgacgcttt ctttcatttt
     6361 tcagcttatt ttacagtttt tcttaatttc taaatgattt tatcaagaaa aggcacggcc
     6421 atatccagcc gtaccttctt gttttaagta tgtgaactaa aggttctctc cattttcttc
     6481 aatcagtttt tggtaccagt aataactgtc tttcttatat cgttccaaag atccttcttg
     6541 tgtttcgtct tgatcaacat atacaaatcc atatcgcttt tgataaccgt tcagccagct
     6601 aagcaaatct gtataagacc aggtgcaata gcctaggact tctgctccat ccgaaattgc
     6661 ttctttgatg gctttgacgt ggctttgtaa gtaattgatt cgatagtcgt catggatttg
     6721 tttatcttct gtcaacttgt catactctcc taagccattt tccgtaatca gtaccggcat
     6781 gcggtaacgg cttgtgatac gacgcagtcc gatccttaat ccttctgggt caatctccca
     6841 gtcccaattt gttcgttcga caaatggatt ctcaactttt ttatacattc caggaactcc
     6901 agtttcttct gatgaacctt tttttcctgt cgtattcatt tttccagcac caactccgtt
     6961 taatggattg aatgcattcg tcgccgtttg atagtaattg atcccaagaa aatctggttt
     7021 agcagattct agcaattctg catcgccagc ttcaatcgtt ggtgcccatc ctttttcttc
     7081 taagaacctc atggcagcaa tcggatattt tccccacata tagacatcca tccaataatt
     7141 ggccatcagg tcttctgcat tttcagcagc cagaacattt tcaggattac tgtcaattgg
     7201 ataatttggc gtatacgcaa agcttggtcc gatttttcca ggcatttcta actcgtgaaa
     7261 tttattgatg acagatgcat ttgctaaatt agcaatatgg ttaacttcat acattcgttt
     7321 cgggtctgtc acacctggcg gatgtgctgc taacaaataa cctaatgaag tgaagatatt
     7381 ttgttcattc aaactaatcc agtaatggac tttcccgcgg aatgcctcaa ataatgtttc
     7441 tgcataatga gtgaaatctt caatgatctg acgtgattcc caaccattat attcatcttg
     7501 caatgcttgt ggaagatccc aatgatataa agtcaacata ggttcaatat cgttttcaat
     7561 caattcgtcg atcaatctct cataaaaagc gagcccagct tgattgactt ctcctcgtcc
     7621 attaggaaaa atccgtgtcc aagctacaga gaagcggtaa gctttcagac cttgttcttt
     7681 cattaaagca atatcttctt tataacgatg atagtggtct actgccaaat caccgttcgt
     7741 tcctttaaat gtctttccag gtatccgtac aaatcgatcc cagactgatt ctcctttccc
     7801 atcttcttgc caagctcctt ctacttgata cgctgcagaa gcagagcccc acagaaaatc
     7861 ttttggaaat gaggtaagtt ctttgtgttc cattcatctc ttcctttcct ataaccttca
     7921 atcgttcctc ttttagaata acacgaaacc ccttccacaa caaaataaaa ttatgtaaa
//
LOCUS       DANKHL010000134         7966 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000134 DANKHL010000000
VERSION     DANKHL010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.138"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..283)
                     /locus_tag="PW101_002064"
     CDS             complement(<1..283)
                     /locus_tag="PW101_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL2514457.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQH"
     gene            complement(273..611)
                     /locus_tag="PW101_002065"
     CDS             complement(273..611)
                     /locus_tag="PW101_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514458.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(601..927)
                     /locus_tag="PW101_002066"
     CDS             complement(601..927)
                     /locus_tag="PW101_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL2514459.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(908..1186)
                     /locus_tag="PW101_002067"
     CDS             complement(908..1186)
                     /locus_tag="PW101_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL2514460.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(1188..2552)
                     /locus_tag="PW101_002068"
     CDS             complement(1188..2552)
                     /locus_tag="PW101_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2514461.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(2565..3134)
                     /locus_tag="PW101_002069"
     CDS             complement(2565..3134)
                     /locus_tag="PW101_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HDL2514462.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(3106..4293)
                     /locus_tag="PW101_002070"
     CDS             complement(3106..4293)
                     /locus_tag="PW101_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2514463.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(4357..6084)
                     /locus_tag="PW101_002071"
     CDS             complement(4357..6084)
                     /locus_tag="PW101_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2514464.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(6081..6533)
                     /locus_tag="PW101_002072"
     CDS             complement(6081..6533)
                     /locus_tag="PW101_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDL2514465.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(6645..7025)
                     /locus_tag="PW101_002073"
     CDS             complement(6645..7025)
                     /locus_tag="PW101_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2514466.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(7022..7408)
                     /locus_tag="PW101_002074"
     CDS             complement(7022..7408)
                     /locus_tag="PW101_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514467.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(7410..7688)
                     /locus_tag="PW101_002075"
     CDS             complement(7410..7688)
                     /locus_tag="PW101_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514468.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
BASE COUNT         2194 a         1655 c         1261 g         2856 t
ORIGIN      
        1 aatgttgggc acgttgattt gttgtgccat tttcggcaaa gcgccagtaa aaggccgtct
       61 cttcaaattg aactttcact tgatcgtttt caactactac ttttatttga tcgcgcatat
      121 gtttcttttt taagagcgac ttaggaatat taggaagcaa ctgctttaga taaaagttag
      181 ctgcttcttc taaagattcc aacgataatt ttttcggatc aacttttgaa aggtttccta
      241 aataatccga tatttcagaa aaaccattct tattacttgg cattttccac acacctcaca
      301 tatgtgtaat agttggttac ggtatcgtcg ttctcatcac cttgtatacc tgtaaaatct
      361 gaatatggaa tgccagcatt ttgcagcgct tgttcaataa caactaaatc ctgttctgtt
      421 ccaagtgtaa aaaaagagac ttggtaataa ggtaattttt tatagacttt accggatgcc
      481 atttttttgc tgttactcac atttgagtac acaatgtatg gatacatcgt ccctaatctg
      541 gctttgtctc tgaacactgc taactttgtt gatttcagcg ctgttttcaa ttcatcaaag
      601 ctaatcgaca taagctaaac tcaactccat ttctcttgca tcgggattcg tataaatgcg
      661 agtaatgtta tacgttacag aatcaatttt gagcgcactt aatttctctg tgatggattt
      721 atcccatctg actttaattc gtctgacaac gtctgtcttg gcttgctgtg ataaatattt
      781 ttcttgagaa gtcacaccga gttcttcata gaatattaga cgcttaaatt cgtaaattgt
      841 agttggacga tcgtttccgt ctgttcctgt tttgatgtct agcaattcgg ctttccatct
      901 gagattatta gtctgtctct tcggcatttt gaatcgctcc ttgcacgata aatggcgtca
      961 tggcattcat agctttgtcg agttcatcct ctgaaactct atattcatag gcaatgccgg
     1021 caaccatcaa aataagatat tcttgttggc caccagttgc tgttttgaca taatcttttg
     1081 ccatatttaa ataaaaagag agcaaagaat catccatgcc ctcttcaaaa tgaatatgtg
     1141 atttgaattt ttcctctaaa gacaattctt tagtttgctc ttccatctta accaccaact
     1201 ggttttgtaa tttcgtagcg atatactgcc ggttcaaatg gagaataaac caattgacca
     1261 tctagcaagt tgtaaatttg gaatccaatt tgatttttac cagagaattt ttcaacaagt
     1321 ttttgaattt ccaaggcacc aataacttct tgaattttaa atgcagaaaa atcgccaaaa
     1381 tataaaactg gcgtgtctgg ttcacccttt ttatctgctg catctgtcca atccacagga
     1441 tagccaacta attggtaacc aattccacct tctgcttgtg tgaatggccg caacaaagga
     1501 aatccatcat ctgttttcat tttttcaatg gcagtcaaag cagctcgatt aataataaag
     1561 cgtccctttt tcatcacttc tgtcactggt gtatttttaa attcgattaa tgcatcatat
     1621 aatttttgcc cagcacctga agcagttaga tctaaaggtt ttttaaatgc tacagccttt
     1681 ttggctaatg caccaggatt ttcatttcca gcgtcatcac cattgaacat ataattgatt
     1741 tctttacgca cataagcttt tttcaattct tccacaacaa tatcttcaac tggaacacca
     1801 gacattttta ataatttttt agttactgtt gccaaagcat cgaattcggc aggattaagc
     1861 aaaatttcat caaactgaat agctgtttca gcaatatcag ttgaacgctc tttcttgttt
     1921 acattcgcat ctgctttctt cacaagaatt ggatatttga catctcctga tgttcgcacc
     1981 actgttccgt atttacgaag taaattttct tcttgagcat aagtaataac ttcagatgca
     2041 attacttctg ggacagtaac tgaaccgttg ccagcttcaa tccctaaagc tcgagcttct
     2101 gcttcagaaa tatttccagc tacaaaatta gcaaatgctt ttcgtagttg ttgatctttt
     2161 tgtttcttgg tcatttttgc acgtgcctcc aatccgttct taattgatcc aagtaatcca
     2221 tctcgttgct gttgagtaat cattccagaa cgattttctg aactatcttc actgtctgaa
     2281 tcttcttctt gatcgttgtc ttcaccagaa cggctttcgt ctgaatccgt actattcgat
     2341 tgatcatctg tattgtcgtc tgtttcatca gttccagaat ctgcgcctaa ttcgtcttta
     2401 attccgttca attcatcaat aacactgtca atttcttctt tcacggcttc taaatctgct
     2461 tcgcgtaatt ctcctgattc aactttttca cgtaattcag tcaatcgttg ctcacgacgt
     2521 gccttcattt ttttcaataa ttctttatcc atgtattttt cctcctacgc ttctagcgct
     2581 tgattaattt ttttgattaa tttttttctt tcttcgacgt ttttttctaa ttcatcacga
     2641 cttcttaatg cagcttctgt atcttcgtat gctggcaaag gcacaataga aacctcgtat
     2701 aattccactt catggattgt tcgaagcatt ggttcagaat tgtagtccca cgtttcttca
     2761 gtcggcacaa aaccaaaact acattggttg atgtctcctc gttccataga tttaactaag
     2821 tcccttgcta ccgttgtgtc cggtaaatca acttcgaatt tcaaaccacg ttcatcttct
     2881 tcaaggcgaa gggttccgct tttagtacgt cctagcacat tggaccaatc gtgattgaac
     2941 aaacaacgaa catccgaatt actaattgcc tttgagaatg caccaggaac aatcacttcg
     3001 cttaagtcat cccataaaag tgtttgactg ttgaatacag cagcgtagcc gctaatggtc
     3061 ctcgtatgag tttcttcatc ggaacgtgtt gaaagattgg tgatgtcaat cgtgcgaatt
     3121 tccttcttct tcatttccat cacctccttt caagtcttga tcattcgttg gtaaggaatc
     3181 atctgttgca tttttctggc caatcctaga taagtcattt gaaatataga tagcttgtgt
     3241 ttctggagta ttctgtttag gaaaaccaag catttctgcc acgttatctg gacttgtaat
     3301 cccggtacga acgatgttgt agccaatatt tgtttttgtt gagtaaggaa caaaatccaa
     3361 aatattaatt ttccattcca ctctatagcc agaattaggc ataaaaaaaa gagcggtgta
     3421 atgttcgctc ttgtttttca atattggttt gattgctttg ttgtgcagat acatcatcgc
     3481 tttttcaata tctgtcttca tcagcgattg atacgtatca acattgattc ctagaaattt
     3541 tcctaagtct tttttgtaaa cacctaaata attcaaaatt gccgcgtcat caacaggact
     3601 ttttaatgtc tcgatggaat atccttttcc caaaggaatc atcttaacag aatgatcact
     3661 ctcattttgc gttccttcca gttgatccaa tatagctttg acaatttttg tttgggcgct
     3721 attatttgga ttgatgtggg cgtctagttt aagcatgaac gcaagtaaac cgcctttagt
     3781 atatttgtcc gtcaaaactt tttcagcgct cagaacgcct tctagagtgt ttcttgcaag
     3841 atcaattatt ccagcacctt ttaatgaatc agttccgatg ttcttaatgt gtcgaatcat
     3901 ttgaccaggt attttttgac cattcatttc aaattcttct tgaagtcgtt cattgatttt
     3961 agttgttaca ccgtacgcca aatgaagctg atccctatcc gttaatggga atgtctcacc
     4021 attgatcaat aaagtatttg tttccaattt ggtaaattcg aatcctgtca aataatcatt
     4081 gggactcttc aaaatattta gtaagtgatg atctttgact tcgttgccgt cgggaccaat
     4141 aacaattggt gtggccaatg ccacctgatt agatatgtct tgaactaatt cataaacatc
     4201 agaagattcc atgatagatg aatccgtcac atatctttga ccataacgtg tgtagtgtcc
     4261 aaaaatatcc tcgatatatc cacgtttttc cataaaagaa tagactgcat tcgataaccg
     4321 atcacgtaat ttcaattttt ttcaccgcct ttctatttat ctatagatgg aacttaggta
     4381 atcatctaac tcatctgaat caatatctgt catttgattc atcgtttcct tatgaccaca
     4441 caaaaacgcc acgaacccat caatcttttt ctttgattga cgtttacttg gcgctttttg
     4501 tccattgatg ttagtaattg ctacaacgtt caaagtgcaa taaaggaata atggattatc
     4561 aaattgaatt cgtttctcat aaaacaaccg ttcgacatcg tcaaaaggag cattcaatac
     4621 tttaggatat tgagcaactt caacgcattc caatcctaag ttttctaatt tctcaacaag
     4681 tttgtcactc atcgctggat cataattcac ttgctgaata tcatataaat tcatgcagtc
     4741 ctctatgtac tgcaaaatct gatcttgatc aatcattttt ccgtcgcaaa attcaacgaa
     4801 accttgttca gccaaatcac tgtaaggaac attatcttct ttttctcgaa actctaaatc
     4861 ttcattcgga ataaaataaa gttgctttac tttaagaacc gctttaccat cttcatccca
     4921 tgttgggaag tttaaagata cacaggtcaa atctcgtgta cgtgataaat ctaaaccgat
     4981 ataacatggt tcgccactta aatcaccaag ttcttgtgtg gtaactaaac atggctctac
     5041 ttgatcctgt tcaaagaaat tatccgcgcc gttcacaaat acatccaagt gtttcgttag
     5101 aaattcagct ttggaatgag ctgaccgttg cgctgtttta aatgccgatt ctaaagcaga
     5161 taaatcgact gatattcccc agttaggatt gcacatttcc cagacttttc tatccgtcca
     5221 atcataattt ttatttggct catagattag aacaaagttt gaatcattat catcacgttt
     5281 caacacttct tttgcttcgc gatatacacg cattccaaca gacgacgaac ctttcccagc
     5341 agttgagata ttaaacatca atggctgtgg taaagatatc tgagcagact taaaattgtc
     5401 atactgttcc attttctcct gtttatgcaa ttcatcgttc aatacaaaat atggattgga
     5461 tccctcgata ttatcaatat tcttggtttg aacaataaat ttatttgagt aagccatatc
     5521 ttcatgaata tagtcataag taatactaga aacagttccc tttggccccc taaatatttt
     5581 agttccttca agtaggattg gattgtttaa gattgtaact gcaaaaggtt tggcagcata
     5641 ttgcgcttga gcaaaatcag atgcgcaagc atagcaatca actgataacg ccccttctcc
     5701 gtacattgca tatcctaatg aaccgacagc aatcaatgtt ttaccattct tttttggaat
     5761 ctggacataa gcctcacgag taacgcgtac aatttggcct ttttcatttt ctttaaccca
     5821 accataaatc caagaataaa taaatttttc ccagggttct aaaagaaacg gttttcccac
     5881 catttctcct tttgtgtgaa caataaatga ttctacccaa tccatcattt cattcgcgcg
     5941 atctacatca aaccaaatat cttttctttt tttccactga taccagcgat ctattgctaa
     6001 acgaacggtc ttaggatact tcttaggttt ctttcttact tcttttgcaa ataaatcagc
     6061 ataatttaca ccaggttcaa tcatttttca gcacctgcct tcttacgcca tttatttctg
     6121 tgcgctgcta gttcatctac tggctttact tctgggcgtg taatttcttc atcttttctt
     6181 gcggtcgatc ctccagcaat tagtctacca gtttttgctt tgtttgttaa tcctaataaa
     6241 tctagagctt tcatcttctt atctgcccaa gtttctactt gttgtgccaa tggatgtttt
     6301 gattgattag tagctcctga tttattcgtg aatttttgcg tctccggaaa tcctttttcc
     6361 ttccacaata aatatttgtg ttggtaaatt tcaaaaatat ccaagtatga ttcgattaat
     6421 ggatcaagag tgatggtgta caaatcagac aaattcatta tttctaagat acgagctttt
     6481 tcagcactta ctttttcatc aataatcgct ttacgttgcg ctttagtcgt catttttgta
     6541 tacacccccc tttgtttttt gaaaaatttg acctaacgat gcgcgtgact ccccgctacc
     6601 ctatctcccc acgcgaaaaa attttgaaaa tgaatagggg ggcttcaatt gaaatacgaa
     6661 ggaaaaactt ttttgtcttc cgtttcgttt tcaacaattg gatgacattt tgaacataat
     6721 agcatgagat tgtttggatc aagcttaagc agttcgttgt ctttgattgg tacaatgtga
     6781 tggacgtgtg ctctctttcc aaatatgaac tgaccacacc gctgacaatg accaccttct
     6841 ctttcataaa taaattgacg catatctttc catgcttgcg ttcgatagaa tggtttgttc
     6901 tcatgatgat aaacagactt tgcttgctgc ttcttcttgc gtgatctgct tgatctcttg
     6961 tgttcagtac agtagatacc ctttgctatt ttgttcgtgc atccgtcaaa ctgacagtat
     7021 ttcattctac ttcacgaata agatttatga tatcgccttt tgcacggata gcaccaggaa
     7081 tatcaatacc atgtttctta gcatatgcac gcaattcttt tgcagtcata ttgtccagtt
     7141 catccgtatc ttcagtggac tgatcattag tagcttcgtc tccatcaaaa ttagcagcag
     7201 tgtttccatc gctatcaaga atttcactgc tatgagctat gagttcgcca ttgacagcaa
     7261 caaatgattt gccatcactc aaaattcctt tttcttcttt tacttcaaaa tttggttctt
     7321 gatctttcgg aacaactact gtccttttct tttctgaatc ccaatactct gttccactaa
     7381 tggatgttct aattttgatc attgccattt taattctctc ctttgtaatt tgtacggatt
     7441 acttttgcgc ccattcgctc ataccattca atcgtttcct gcagattggg tagagtgtgt
     7501 gacaataaag aaataactaa cgtattcatt ccagttctgt aatcatcatt ctccacttgc
     7561 acatgtgcat gattaccatt ccaattttct ctgtacggac tattctctgc ttcaattgct
     7621 ttcttataaa ctttagccaa acttgattct acttcaatac taagtaccgc ttcatgaaaa
     7681 tcattcattt gtttactctc ctatcagata ttttgtggta tgatttataa aaaagatgcg
     7741 aggtgtttta atgcttcaag aagttaatat taagtatcgt gatcgtcaaa atacagagac
     7801 aatttatttg ccaaatagtt gtccgcattg cggaaatata atgactccgt atgtgtattt
     7861 tggggtttca tcatatagtg cgttcgataa tgatagaatc tttggcgtat tagtacagtg
     7921 tataaatgat gagtgcaaca aattttatag tttacaattt ataaat
//
LOCUS       DANKHL010000135         7886 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000135 DANKHL010000000
VERSION     DANKHL010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.140"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            74..1165
                     /gene="phnW"
                     /locus_tag="PW101_002076"
     CDS             74..1165
                     /gene="phnW"
                     /locus_tag="PW101_002076"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDL2514469.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            1158..1286
                     /locus_tag="PW101_002077"
     CDS             1158..1286
                     /locus_tag="PW101_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDL2514470.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            1290..1835
                     /locus_tag="PW101_002078"
     CDS             1290..1835
                     /locus_tag="PW101_002078"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDL2514471.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(2289..3980)
                     /locus_tag="PW101_002079"
     CDS             complement(2289..3980)
                     /locus_tag="PW101_002079"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDL2514472.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(4400..5347)
                     /gene="arcC"
                     /locus_tag="PW101_002080"
     CDS             complement(4400..5347)
                     /gene="arcC"
                     /locus_tag="PW101_002080"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDL2514473.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(5462..6481)
                     /gene="argF"
                     /locus_tag="PW101_002081"
     CDS             complement(5462..6481)
                     /gene="argF"
                     /locus_tag="PW101_002081"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDL2514474.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(6572..7801)
                     /gene="arcA"
                     /locus_tag="PW101_002082"
     CDS             complement(6572..7801)
                     /gene="arcA"
                     /locus_tag="PW101_002082"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDL2514475.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         2238 a         1608 c         1462 g         2578 t
ORIGIN      
        1 atttatattg gatgttaaca agagcagctt cttgctcttt gtgtatttca aaaaagaaat
       61 gaaaggaaat catatgtata aattattaac ttctggacca ttgacgactt ctgaaacagt
      121 gaaaaatgct atgctgatcg atcattgtac ttgggatcaa gaatacaaag aaattacaca
      181 atggataaga aaaaaattat tggatcttgc tcaagtatcg gaaggaatat acacaacggt
      241 cttaatgcaa ggtagcagct ccttcggggt agaatcggta ttaagttctg tgatcaggcc
      301 agaagatact gtactgattt gttcaaacgg cgcatataga caacgaatga tcaacatgtg
      361 cgaatgtctg aaactgaact ataccgttta tgaggtatcg gaacatcaaa tcctccaagc
      421 tcatgtgatt acacagctga ttgaagagaa tccgtctatc tccgcactag caatggtcca
      481 tagcgagact acttccggta ttttgaataa tttggaagag attgctgctg tcacacaaga
      541 aagagatctt ttgtttatag tggacgcgat gtcaagcttt ggtgggatac cgatttgtgt
      601 agccgaacta gggattgatt ttttgatttc cagctcaaac aaatgtattc aaggtgttcc
      661 aggcttttct tttgtacttg ctaataaagc ggaactggaa aaacgaaaaa atgttagccg
      721 aagtttgtca cttgatctat atgaccagta ccgcgtgatg gaaaacgaga atggcaaatg
      781 gcgtttcact tcccctaccc atacggtttt ggcttttcaa caagcgctga aggaactaga
      841 ggcagaagga ggaatcgaaa ctcgatattc acgttatctc gccaataatc atgtgctacg
      901 acaaaaaatg acagagatgg ggttccagcc tttcattcaa gaatatcagg ggccattcat
      961 cacctctttt ctttacccta aggactggcg atttgatttt aatcattttt atcagtattt
     1021 aaagcaaaat ggatttgtga tctatcccgg gaaaatcagc gatcatgaat gttttcgtat
     1081 cggaaatatt ggcgagatct atccggaaga tattttggaa ctgacagcaa ttatcaaaga
     1141 ctataaggag aacgttaatg attaaaactg taatttttga ttgggcagga acaactgtgg
     1201 actttggctg tatggctcct gtccacgctt ttagaaatgc ttttctagaa aaaggaatcc
     1261 aactgactga caaagaaatc cgctaaccga tgggaaaatt aaaatgggat catatccaac
     1321 agcttctttc cctcccttct gtcaaaaaac aatggatagc tatttatgga caactacctc
     1381 aggaaaaaga ggtggaggaa ttgtatcaac aatttgaaca ttatctgttt aaagatttgg
     1441 tcaagcattc taccttgaaa cccgacacat tggaaacgat cgaacggttg agaaacgctg
     1501 ggatcaatat tggttccacg aatggattta atgctcctat gatgacaatc gtagcagaaa
     1561 ccgcaaaaaa agaaaggtat tcaccagact tcatggcaac ttttgaagac gtcgaagggt
     1621 atggtcgacc gtttccttat atgatccata aaaacatgca gtacttcaaa aacaagtcgg
     1681 ttgatgaagt aatcaaagtc ggagatacgg tctcagatat ccaggaaggg aaaaatgccg
     1741 gagtgttgac agtaggtgta attgaaggta gttcgctaat ggggatttct tatgatgagt
     1801 accaaaaatt agagatgggg aaaaagatcg tttaaaacag tctgttgctc ttagattcca
     1861 acaagccggc gctgatattt gtgtaaacaa tttaaaagag ttaacagaat acattttgta
     1921 cgctgacatg aatccagaat gacttttata caaaataaga ggttgtgaaa aagctgtcct
     1981 gtatcaagta ataaaaacag ccaagcaaaa atagctcctc atatttttcg tttggttagg
     2041 cttattaccg cagatgcaag cttttgaaca ccgtttattc gaaaaaaaaa agggagctgt
     2101 gacacgactt ttgtcacagt ccccttattt gcaaacaaac agtgtttaag agacaacctc
     2161 tccactaaac ggtgttcatt cagctgactc tcggtatttg ctcaaaaaac gataaaattt
     2221 gaaaagctat tttctctttt gtttctctaa tactcggatc agaacgctga tttcacattc
     2281 tctttttctt acatcttatc tggtgcatga agtcctagca aacgaaggtc ttctttcaat
     2341 aatacagtca ctgcataaac tagagctaag cgtgcttctt tttgttcatc atcagctaaa
     2401 atcttcgtat gagcatagta tttgttgaat gcttgtgcta atttgattgc atgtttcgca
     2461 atcactgacg gttcgtattt ttccccagca gacaagaccg tttcagggta tttttgaact
     2521 aatttaacca cttcccagct ggtatcgtca ttcaatgcat agtctgctgt ttctgaaggg
     2581 acaaatcctg ctttttccaa caggctgact gcacgagcat gtgtatattg cacataaggt
     2641 ccagtttctc cttcaaatcg aacaacttct tccaggttaa agtcaaatgt attcagtcga
     2701 tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa cttgtttagc tacagcatct
     2761 ttgttttcca aatcagggtt tttctcgctg atttgttctt tagcagattg gattgcttca
     2821 tttaatactt cttctaataa gacgatcttt cctttacgag ttgataattt tttaccacct
     2881 tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag accagtcaaa gcccatttcc
     2941 ttcaagacag ctttcaactg tttaaaatga tagctttgtt cattccctac tacatataga
     3001 gattgtttga agtcgtaagt acgtttacgg tacaatgcag cagctaagtc acgtgtaatg
     3061 tatagtgtcg caccgtctga cttcttaatc aacgctggat tcaggtcata agcagatagg
     3121 tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt tttcttctaa aagtttgacg
     3181 atttcatcca tcttgtcatt gtagaaagct tcaccgttca acgaatcgaa acgtacttcc
     3241 agcagatcat agattttatt gaattctttc atcgattcat cacggaacca ttgccataat
     3301 tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc aagcacgagc ttcttcattt
     3361 aattctggtt ctgtttcagc tacttcatgg aattggacgt ataaacgaag caattcattg
     3421 attggttctg ctttaacagc ttcttctgta ccccattttt tgtaagcgac gatcagtttt
     3481 ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga ttggctgata accgattttt
     3541 tccataatga aaccgatcga attaccgata actgtcgaac gcaaatgtcc cattgagatt
     3601 ggtttggcaa tattaggaga agacatgtcg attggtactg ttccttgatt cccgatgttg
     3661 ctatccccat aatgttcttt ttctttcact actgtttgca aaactttttt gctgataagt
     3721 tctttattca tgaaaaagtt caagtaaggt ccgacaactt cgattttttc aaagttggca
     3781 gaatcgattt tttctgctaa atctgcagcg atttgctgtg gcgcttttcg gtaaactttt
     3841 gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag ctgattttgg attttctagt
     3901 aattgctcga cttgttccag tgttaaatca tctttgacag cgtcatagac agcttttgcg
     3961 acaagatctt tattattcat ctttattcct cctaaagtgt tcccttgatt gttaaacaaa
     4021 aaaaatctct agcgacggca tacttagtat gccatcacta gagacgagat ttcccgcggt
     4081 accactccaa ttgtcctaaa aaaggaccag ctccatctga tatcggtaga cgaccgtctg
     4141 tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc ttcattcggc ttccaccact
     4201 tccgaattcg ctagccaaga atgatacaag tactctcttg ttcaacaaat cttagtctta
     4261 ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg ctagtcgttt cataaaaaaa
     4321 caatccagat ggcctaaagg agaagaaata ccacctggat agctataaat catttcgtaa
     4381 atacgaaaga cttaacaaat taatcagcta cgataatcgt tccttcttca gaagctaaaa
     4441 gattctcgat attttctaaa gaagtgatga ttgccttagc gtttggtttt gcttcaacaa
     4501 atgcaatagc tgcttctact ttcggaagca tgcttcctgg agcgaattgc ttttcatcaa
     4561 tgtattgttt catttctgaa acagtaacag tttctaattt cttttgatct ggtttttggt
     4621 aattgacata cacattgtct actcctgtca atacgatcag caaatccgca tcgatgattt
     4681 cggctaattt ttctgaagca aaatctttat cgattacggc ttctcgtcct tctaatccag
     4741 tagctgtttc tacaacagga atcccaccgc caccaacaga gactgtgata actccttgat
     4801 cgaccagcgt ttcgatgaca cgtgcttctt taatcgagat gggtttaggg gaagcgacta
     4861 cttttctcca tccacgtcca gcatcttctt tgaatgtcac agtggagtca gcattcattt
     4921 gttctttggc ttcttcttcc gtgtagaatg gaccgactgg tttacttgga tttttgaatg
     4981 aaggatcatt ttcatccaca ataacttgag tgactaaaga aaccacatct ttatcgatcc
     5041 ctttttcctt cagtacttcg cccatggcgt tttgtaacca gtaaccgata gatccttctg
     5101 tcattgcaac acatgtatct aaaggcattg ccggtgtttt ctcagaatca gcagcttgct
     5161 gctgaatcaa taggttaccg acttgcggac catttccgtg ggagatgatc aattcatctc
     5221 cttgttcgat aaatttcact aaatactttg ctgtttccat taacgcttct tgttgggctt
     5281 ttgcacttgc atccgtagac aagatggcat tgccccctaa agcgacaact acttttcgtt
     5341 tggccatttc attcgtcctt tcttcagttc taccgaattt tctagataac aaaacgagcc
     5401 agcgttttgt gattttccct caatgctgac tcgttttttc gtgagatgaa tgtggttatg
     5461 cttaaacgcg agggataaac aagttaccta atgttgctgc catgatcgct ttgattgagt
     5521 gcatacgatt ttctgcttgg tcaaattgac gagcatattt gctgcggaag acttcatctg
     5581 ttacttccat ttcagtgatg ccaaagcgtt ctttcatttg ttcaccgtaa acagtgtttg
     5641 tatcatggaa agctggcaag cagtgcaaga agatcaaacg atctgtgttg tgtgtttttt
     5701 ctaccatttc catattgatt tggtaaggtt tcaacaattt gatacgttct tcgaatttat
     5761 cttcttcacc cattgatacc catacatcag aataaaggac gtctgctccg tctacacctt
     5821 tatcaacatc gtcggtaatc atcaattgtg caccggattt ttcagcaaag ccttctgcca
     5881 ttttgacgat ttcttcttca ggttgtaatt cttttggtgc aacgatacgc atattcgctc
     5941 ctaaaattgc acctgtgact aataaagagt tagccatatt gtttcgacca tctccgcagt
     6001 aagcaacagt gataccttct actgttccaa agttttcttg gatagttaaa aagtcagcga
     6061 tcatttgtgt tggatgccat tcatcagtca agccattcca aactggaaca ccagagaatt
     6121 ctgctagttc ctctaccatt ttttgactga atccacggaa ttcgattccg tcaaacatac
     6181 gtcctagtac tttagctgta tcttctgtgg attctttttt acctaattgg atatcatttg
     6241 cacctagata ttcaggatgt gcacctagat cgatcgctgc agtagtaaat gcagaacgag
     6301 tacgtgtaga tgttttttca aataaaagcg caatattttt tccttctaga tagtgatgtg
     6361 ggataccacg ttttttcaag tctttcaagt gttctgaaaa atcgatcaaa tattgtaatt
     6421 cttcttttgt aaagtctttt tctgctaata agcttcttcc ttggaatact gattctttca
     6481 tattaaacat tctcctttag attgttttta tttaagatta aagaggaaat cccgacggca
     6541 gctgacagta agactgccgt caggaaacaa attacttcaa gtcttcacgg acaagtggtt
     6601 ggctcatgca gcgtggacca ccacgacctc gtgacaattc acttgaattg atttctaaca
     6661 ctttgatgcc atagctacga agcaattcgt tcgatacata atttcggtta taagtgacta
     6721 caacacctgg agcgattgcc aatgtgtttg atccatcatt ccattgttct cgaggtgcaa
     6781 cgatctcatc tccattacca gttgggatca gtacaagatc atctaatcca agagcagctt
     6841 tcatcgtttc gtgtaagtta tctgaatgag taatcttgat atcatcacca tctggttcga
     6901 ttgtaaagac atccatctta ccgtctttac tttgaattgc tggatggata gtgaatttat
     6961 cgtgatctac cattgtaaat acggtatcca aatgcatcat tgcacggttg ttcggaattt
     7021 tgattgccaa cactttttca aatcctgaat tttttgcgaa taggtggcgt gctaattttt
     7081 ctaaagcttt cgcattagtt cgttgagaaa tcccaacagc tactacttta tcgcttaaga
     7141 tcaattcgtc tccgccttcg atatgatctg gattttcacg atccaaccat acttcgattc
     7201 ctttatttgc aaatcttgga tgatgtttca aaatatattc tgtaaacatc gattcacgac
     7261 gacgtgcttc aaaagtcatc ttattgactg tcatcccatt tcccattgat gcggaagggt
     7321 cgcgtgtaaa gtacaagttt ggcattgggt ccatgtaaaa tggatagtca tcgtctgctg
     7381 acagatcata tagactgcta gaacgtactt cgatatcttt tgtacgaacc ccagccatga
     7441 ttttatctac catttcttgc gtttccattg atagaaggaa ctcatgaaga ccctctctta
     7501 cagcattcga tgcaatatga gattcatcca acattttatt caagaattgt tctttgacat
     7561 tgcctgcgtc aatcgcttct gccgctaatt tttccaagta aagagtctcg acgccttcat
     7621 tttgcaatgt cttcgcaaag ttgtcatgtt cttcttgagc aatcggaaga tatggaatat
     7681 catcgaacaa cagacgatcc ataatatctg gtgtcaaatt ttccacttct tgtccaggcc
     7741 gtttcagtaa aactgtcttt aatttcccta tttcagagaa aacgtgaata ggtttatcca
     7801 tgttgtgttg cctccttctt tttgattaca tgtataggat atcctccttt tgtaatcact
     7861 ttcataattt tgtctcattc aaaatg
//
LOCUS       DANKHL010000136         7803 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000136 DANKHL010000000
VERSION     DANKHL010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.141"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            108..971
                     /locus_tag="PW101_002083"
     CDS             108..971
                     /locus_tag="PW101_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2514476.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1100..2722)
                     /locus_tag="PW101_002084"
     CDS             complement(1100..2722)
                     /locus_tag="PW101_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2514477.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2971..3492
                     /locus_tag="PW101_002085"
     CDS             2971..3492
                     /locus_tag="PW101_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL2514478.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3678..5408)
                     /gene="spxB"
                     /locus_tag="PW101_002086"
     CDS             complement(3678..5408)
                     /gene="spxB"
                     /locus_tag="PW101_002086"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDL2514479.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5573..6391
                     /locus_tag="PW101_002087"
     CDS             5573..6391
                     /locus_tag="PW101_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDL2514480.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6494..6691)
                     /locus_tag="PW101_002088"
     CDS             complement(6494..6691)
                     /locus_tag="PW101_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514481.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6860..7438
                     /locus_tag="PW101_002089"
     CDS             6860..7438
                     /locus_tag="PW101_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL2514482.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7533..7670
                     /locus_tag="PW101_002090"
     CDS             7533..7670
                     /locus_tag="PW101_002090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514483.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2339 a         1387 c         1551 g         2526 t
ORIGIN      
        1 attttgatta gaacttgcga aaataaaccg gttaaagtgt gtctagcgca agagatttgc
       61 tataatagac gataagggta aaatgtaacg agaaggggtt gaaaaatgtg tatcaaattg
      121 taacagattc ttgttgtgat ctgccatacc atgtattagc aaaagaaaaa gttgattttt
      181 tatcaatgaa tctccaattg aatgatcaag agttagtaga tgacctcgga aagacattca
      241 attatgaccg ctacatgaat cagctgaaaa atggtgcgat gcctacgaca tcacaaatca
      301 atgttggccg atatttagaa tttttccgtt cttatgcgga aaaacagatt cctgttttat
      361 atgtggcttt ttccagtgga ttaagcggct cttacagtag tgcgcttcaa gcggttgaat
      421 tgctgaagga agaatatgaa gaccctgaga tctatgtttt tgatacaaaa gctgcaagta
      481 ttggggaagg gcttttagta ttggaagctg taagattgaa aaaagaaggt aaaagcatcg
      541 aagaaaccct agagtggctg gaaagtaatt atttgaaagt ccattcatgg gtaacagtag
      601 acgatctgaa atatctagaa cgaggcggta ggatttccaa agcaagtgct actcttggca
      661 gtctattgag tgtaaagcct attattacgg tggataaaga aggaaggctg acgaatgttg
      721 acaaagttcg aggaagaaac aagtcgatcc agacaatcgt caatgagacg gtaaaaacaa
      781 tcgttgcacc acttgatcaa atcgtttatg tggcttacgc tggggatcta gagagcgcgg
      841 agaaggcaaa acggcttttg gaagagcaga taaagatgaa agatgtgaag ctttatccct
      901 taggaccaac gatcgccagt cacacaggct atggctgtat cgcgattttt tcgatgggaa
      961 tcagtcgata aaagcagtaa ctcattctgt aaaaaagccc agaaattgat tgtaacaaaa
     1021 acagcgactc ttaaaagata gatgagcatc ctatctctta gaggtcgctg tttttttatt
     1081 ttttaagcta aataggccat tatgcccata actcgttcaa ttgtttttga atatctgcat
     1141 tttccaaaaa ttcatcatat gtcgtatcag ctcgatcgat cacaccttta tccgaaacgg
     1201 caatgatgcg gtttgccaat gtttggataa attgatggtc atgtgacgca aataagattg
     1261 agccagtaaa tgccatcaag ccgtcattca atgctgtgat tgattctaag tctaaatggt
     1321 tcgttggatc atctaataca agtacgttag ctttcgaaag catcattttt gaaagcatgc
     1381 aacggacttt ctctccacca gataaaacat tcactggttt caatacttct tcaccagaaa
     1441 acaacattct tcctaagaag cttcttaaga aggtgttatc atcttcttct ttacttgcat
     1501 attgacgcaa ccagtctaaa atcgttagat cagtatcgaa ttctttcgtt gtatcctttg
     1561 gtaaataaga attacttgtt gtcacacccc aacgtactgt tccagtatct ggcgtaattt
     1621 cacccatgat aactttgaat agcgttgttg ttgttatatc attttctgcg acgaaagcga
     1681 ccttgtcttc tttgctcaaa ttaaaagata tatcatctaa aattttcttt ccatcaatcg
     1741 ttacacttac attttctacc tgcaataagt cgtttccaat ctcacgttca ggtttgaatc
     1801 caacgaatgg ataacgtcta gaagaaggtt ggatatcgtc taaagtaatt ttttcaagca
     1861 tttttttacg tgaagttgct tgttttgatt ttgatgcatt cgcactaaag cgggcaataa
     1921 attcttggag ttctttgatt tgttcttctt ttttggcatt tgcgtttgct tgaagtttag
     1981 ccgctagttg actagattcc aaccaaaaat cataatttcc tacataaagt ttgattttac
     2041 caaaatctag atccgccata tgtgtacata ctttgttcaa aaagtgacgg tcatgggata
     2101 caacgatcac agtattttca aaattgatca aaaattcttc cagccaattg atcgattgga
     2161 tatccagacc gtttgttggt tcgtctagca aaagaacatc aggtttacca aatagtgttt
     2221 gtgctaataa cacttttacc ttttgtccac cagttaattc actcatttgc tgatggtgca
     2281 attcttcagg gatgttcaga ccttgcagta aaacagctgc ttctggttca gcttcccatc
     2341 cattaagctc agcaaattct ccttctaatt cagctgctcg aataccatct tcgtcagtga
     2401 aatcctcttt catgtaaatc gcatcttttt ctttcagcac ttcatacaga cgcttgtgtc
     2461 ccatgatgac tgtttcaagt acagtatagt cttcatagtc gtaatggttc tgtttcaata
     2521 cggccatccg ttcatctggt cccatagtaa cggaacctgt ggaaggctgc agatcccctg
     2581 ataacacttt aaggaacgtt gatttacctg caccatttgc accaatcaag ccatagcagt
     2641 ttccgggggt aaatttaata ttcacatcat caaatagttt gcggtctgaa aagtgcaaac
     2701 tcacatcatt tacagtaatc aatttttttc ctcactttta tcattattag ttttgacaag
     2761 tcttatagaa ttatataggg gaaaggtaaa agattcaagg caatcgaaca ttctttgtgt
     2821 ttcacgtgaa acagaatgaa aaaaataaaa ataaaagcgg agcgaaaata gctactatag
     2881 gatttattag tttgcaacag cataattttt gacgttattt ataagaggga gtactataaa
     2941 actgtaaaag aaatgaaaga aggattgaaa atggatgaaa aaagaaccat cgactggggc
     3001 tcattgattt tgggaatttt atttgtactt gtctcattgt tatcatttca agaccctgtc
     3061 ggaaatttag ttgcaattgt agtagtcttt gcgatttttg cctttattaa agggatattt
     3121 gaactgtttg tacggaacag attaaaagag ctaacaggat ataaaggaaa gatgccctta
     3181 ataatcggga tcatcgatat tctagttggc gtctttttct tattcaatat tggtgcagga
     3241 gtcgcagcat tgccttttgt ttttgcagta tggtttattg ctgattcagt tttagcgtta
     3301 tttactgctg acttggcaag aggagtaagt gaaggatatt attggtttac gatcatcgtc
     3361 aatattttag gaattttatt aggaattttc ttattgttta atccaatttc ttctgcgttg
     3421 acattaagct ttttagttgg attctatttt atgttatttg gtatcacaca tatcgtctat
     3481 gcatttagat aagtaaaatc aaacaaataa aaaagccggc ttccgattac agttttactg
     3541 ttgtaattgg aagccggctt ttttatcatg gacaaatatg cacgaggtga tttttaggtg
     3601 tcgagacaaa tttatgcaaa aatgtgagaa gctatataca caaattttat cttaatactc
     3661 gaagcatagc gcagttgtca caacctcttt tcatataatt cattcaatac tggcaaatca
     3721 tggacttcat atttttcttt gaatgcttgg atttcttctt cagaatattt tcttggatct
     3781 aaatgcagat cttctactgg caatgggcgc ttgttattga tcttcacatc gattactaca
     3841 ggacgatctg attttgcagc tgcatcaaag gcaaatcgaa gttgcgagta ttctgtgact
     3901 gtaaagctat ttgccccgag cgcttctcca actttaccga aatctgcatc ttctaaaaat
     3961 acaccaaatt tcttttgttc cgtatcttct tgttctgctt cgatgaaacc aaaagaatca
     4021 ttcgaaaaga caacattaat aattggaagc tgatatttga cttgtgtaat gatatcttgc
     4081 atgaccatag caaacccgcc atcaccactt aaggtaaata cttgtttttc tgggtagctt
     4141 agttgtgcgg caattcctcc tggcacacca ttacccatag ttgcgaacca tccagatgtc
     4201 gtatgttttt gtttaccgtt catattcaat aaacgaatag aatggatagt cgtgttaccg
     4261 acatctgtga cgaaaatcgc atcgtcttca gcaatacgat tgatttcttt atagacagcc
     4321 tctggacgaa tcggttcttc ctctcgatct tcaaaagatt tcaaccaatt tacccatttt
     4381 tctttatttt cttgatttgc taggtaccag ctatcagcag ggcgagcatc ccctaactcg
     4441 acaagtcttt gcaaagctgt gtttgcatca ccgaggatcg acaaagatgt ttcatggcgt
     4501 cgtccaaatt ttgacgcatc gatatcgatt tggataaatt cagcagttgg attaaaaaat
     4561 gcacgtccaa atgggaagtc gctaccgaca aataagatca gatcggcttc tgccaatgct
     4621 tcatttccgg gtttagtagc gacacgtcca gcaaaaccaa gaaagttttc ataatcatca
     4681 ggaataattc cttttgctag aacggaagcg acaacaggaa ctgaaaaatg ttctgagaat
     4741 tctttgattt gcggaaatcc gtttcgcgtt ccttgaccaa cataaagaac tggttttttt
     4801 gctttttcaa gataaggcaa agcagctagc aaatcttttt cttctggtaa tatcacactt
     4861 gttttgtgag tgtgagcggt ggagaaaggt tgttcgtcaa tttcttcaaa accaagatca
     4921 acagggatag tcacgatagc cacgccttta tgctcatagg cggctttaat ggcttcatct
     4981 actacatgcg gaagactttg tggtgtcatg actgtacgat tgtatacgct aacatctgcg
     5041 aaaataggat tttcatttag ttcctggaaa gagttatagt tcatggatgt agaagcaact
     5101 tgtcctaaga acgctactag aggcacatgg tccatctgtg catcatacaa accattgatc
     5161 aaatgtgtcg caccaggtcc tgcagagccg aagacagcgc cgactttccc cgttaatttt
     5221 gcatctgcag cggctgcgag tgcgccgact tcttcatgac gaacttgaat gtagttgatt
     5281 tcggtttttt cttgatatag cgcatccata attgaattga aagatcctcc gggaatgccg
     5341 taaatatggt cgatacccca gctttcaaga acttttacca ttgcgattcc tgcattgatt
     5401 tttgacatgg tgtttcactc ctttagcaat gatgatacat aaattttttg tatctagagt
     5461 atagaacttt ttatcgaaaa tcccaactaa ttcgtctttt cacaaaaatg tagtcatgtt
     5521 tttgggaata tgctagagta gtacatagat agattcaaga gaggggaatg gcatggttga
     5581 atggctattg agacttgaac cttggcagca agcacttgct gggacagctt ttacttattt
     5641 tatgacagct cttggtgccg ggttggtttt tttctttaaa gaaatcaaaa aagaagtgct
     5701 aaacttgatg ttaggatttg cttctggtgt catgattgct gcaagttttt ggtctttgtt
     5761 agatccggct attacaaaag cagaagaaaa tggagatatt gcttggctag ttgtaagtat
     5821 tgggtttggt ttaggcggtg tgtttttata tatggcagat aaaacactgc cccatatgca
     5881 ttttggacca caacatgaag cagaaggatt gcctacccat ttaaaacgga cgatcttgct
     5941 tgttttttct attacattgc ataacattcc tgaaggttta gccgtggggg tagcattcgg
     6001 tgcagcagca actgcggata atccgacagc cgctatatta gccgctgttt cggtggcgct
     6061 aggtattggt atccaaaact tcccagaagg agcagctgta tcaatcccgt tgcgacaaga
     6121 aggtctgagt cgtaaaaagg cctttgttta cggacaagct tctggaatcg ttgaaccaat
     6181 tgctggagtg attggagcgc tactagtaac gaaagtggag ctgctgcttc cttatgcact
     6241 tgcttttgcc gcaggagcaa tgatttatgt cgtggtggaa gaattgatcc ctgaagcaca
     6301 gcagacattg tccagcaaac atcattttgc agtttttggc gtcatgagtg gatttatcat
     6361 tatgatgatt ttggatgtgg cattaggata aataaaaaaa atatatatga tatatcaaat
     6421 agggccgcga acttatatcg cgaccctatt tttttagtaa gttatatcat aatgaagtga
     6481 atggtatcat acattaataa tagttttttc tatgtgtatt tcctttgatc cagcgaacaa
     6541 gccagatgat aagtgcgatt gggattagaa ctttgaccgc aaaccataaa agcccgctta
     6601 ggacactgac aaaaagactg atgacgaagc tagctgcaat tagcactaaa agaaccatga
     6661 agatatttaa gaacatattt ttttgacgca tacaaaaccc tcctcttgtt cattctttac
     6721 tatagaaaaa gtataacagg gattgtttaa aaacacctcc aactgaagac tgatttttac
     6781 ttcatctact taaatgatca aacaattgct ttttctctgg tttatcatct aaaatagagc
     6841 ggaaaaggag gagaatatca tgcctaaaaa tcgtcttgaa gcatttacag atgctgtaat
     6901 cgcgattatc atgactattt tagttttaga acttcatgaa cctaacggag acacgtggga
     6961 ggcgctcgct gcattaggtt accgattttt tatctatatt gtcagtttta tcattttagc
     7021 gatttattgg aacaatcatc atcatttatt tcaagttgta cataagataa atggacgggt
     7081 cttatgggcg aacagtttct ttgtttttgc gctttcgctt tttccgtttg caacttcatg
     7141 gatcagtgag tacatcaata gcctggctcc agaaatgact tatggttttg ttaccttgac
     7201 cgctaatatt gcgtattata tcttggcaaa agaacttgtc aaagcaaata aaggccacga
     7261 gaaagcattg aaggcgatgt tgaataatta caagaaatcg tatctatcga ttggtttgaa
     7321 tgtgttagga attgtcttag ggtatttagt agcgccagta gccgtgctga tcgcaaacat
     7381 cgtgattcta gctgcttgga tcatcccaag tcgtaccatc gaatcacaat atcggtaaaa
     7441 tgatccgtgt ttaaaattta agagaaaaat atagaaaaaa taaataaacg atcaaatatc
     7501 cgagtgatac tagtattcaa agagggatgc agatggatca aaaaagaatt catgtaggac
     7561 tgattactag ttttgtcacg atgcttattt atttggatca aggtcatttg acttggacag
     7621 tagttggtgt cgtaggattt ttgacattat tcacagcaat aaaaaaataa acatctttat
     7681 gtaatctgat gttctatcta tttaagaaaa gcatgtttac agcagtatga ttctgttgta
     7741 aacgtgtttt tatttgtgga aaatttaatt tttagaaaag tgtatcgaaa aatagatagt
     7801 tag
//
LOCUS       DANKHL010000137         7787 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000137 DANKHL010000000
VERSION     DANKHL010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.142"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(69..1490)
                     /locus_tag="PW101_002091"
     CDS             complement(69..1490)
                     /locus_tag="PW101_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2514484.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(1733..3610)
                     /gene="tdc"
                     /locus_tag="PW101_002092"
     CDS             complement(1733..3610)
                     /gene="tdc"
                     /locus_tag="PW101_002092"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL2514485.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(3901..5157)
                     /locus_tag="PW101_002093"
     CDS             complement(3901..5157)
                     /locus_tag="PW101_002093"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDL2514486.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(5269..5474)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5737..7038
                     /locus_tag="PW101_002094"
     CDS             5737..7038
                     /locus_tag="PW101_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL2514487.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(7107..7706)
                     /locus_tag="PW101_002095"
     CDS             complement(7107..7706)
                     /locus_tag="PW101_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2514488.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         2308 a         1592 c         1364 g         2523 t
ORIGIN      
        1 aaaactattc acttggcagg acctcctact aaatcagcga caaaacgtcg gctgaaagac
       61 ttattttttt atttttcttc taaacttttt tctacctcaa tcccccaaga atctttcttg
      121 aagtgattga tgatcaatgg aataacgaac atgaccactg tgatagctgc tactaataat
      181 gcatcactca atgtcgctgt acctaatgta gcaaatacga caacgatgat agagaagatc
      241 agcatgcagc tgactaccca agctaatccg tttccgtttt taccaattcg gtaaggacgt
      301 tcgaattctg gatgtttctt acgcatttta agcaatgaaa cagcaatcaa aatgtacacg
      361 atacaataaa taacagttgt agcatttgta agtgtcaaga atacagcact tacgtcatcc
      421 attacaccat aaagcaaagc aaataatgaa ataacgattg attgtgttag aacgacattt
      481 ctagatacgc cgtatttgtt ttctttatgg aatccgaatt ttggtggaag gaatccttca
      541 cgagctactt ggatgattgt ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc
      601 acaccaataa ataccatgaa gctgaagata tttccgatca cttctggtaa gcctaggatt
      661 tgacagtcaa tcaagatagg ctgcgtgata tttgctaatt ccatttttcc atctggtacg
      721 acatttgcta caagcatcgc attgatgaca ttcaataaaa ctaatccgat caaagcgata
      781 aatacgcctt ttgtataatt ttttgttgcg tctttcaaac gaggcatgta aacagaactc
      841 atttcgatcc caacaaaaat aaatgtgata ccagctaagt atttcaatga atcaatatgt
      901 tccaagtcag ggaaagcttt tgaccatgaa aagtcaccta gatagccgtt tgagactagt
      961 ccgactttga aggctgccaa aacacctaaa acgaacatga tgactactgg gatatatacc
     1021 cctagccata caccgatgtt cccgccgact tttgccatat cgaatttcaa gttcaagatc
     1081 gtgatgatcc agtagataac taaaatacaa cctaaaacga accagtgatt attgccaagt
     1141 tctacattac caaatgtgtt tcctaacaat ggtcctaaag tagaagcgac cattaccata
     1201 cctgggaaca tttgcaccca taatagccat gcaacgacaa atccccattt gctgcccagt
     1261 gctgttttta cccataactg aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg
     1321 gaaaccattg atatcggacc ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat
     1381 gtccaaccga ctgcggctaa cgtaggaata ctccgaaccg ttgcacacag tgccattgtc
     1441 agaccgataa aggtcccaag acccatttta cttacttcag atttactcac cgttgacact
     1501 ctccttgatt ttttggataa aataacggtg tctttgaagt agtttcagca ataaacgaaa
     1561 caattcagcg aatatttgag gggattcttt gagtttattg cttgaagggt ttcttcaaag
     1621 acaacctcaa aacaaaggga tcgaagagct taggtctccc cttgctcttc agatccaaac
     1681 atgcgtcaga aacagttagt attcaagctg ttttctaaca tgattacctt gcttattttg
     1741 cttcgcttgc caataattgg tctgcgtaga ttttttccaa tttttcttga agagctgctt
     1801 tgattttttc agcatattct tcaaagtgtt cttctttgtt catgtatggt gtcattacgc
     1861 tggcacgcaa tacagttact tttccggcac ggttccattc ttcatctgag aatcctagtt
     1921 ggttcacaaa ttgtaatggg ctgttgccat agtctgggat agcaaagtca gtatgtgaag
     1981 ttaagaactc attaccgtaa atgcttcctt tcacgtatga agaataatca tatacgtcat
     2041 ggttcaattt attcattgca actaagtcat cgttgccttt ttctttgaat acatagtcta
     2101 ccatattgaa atctggataa gttaatggat gaacttcgat ttctttgtct cctactttaa
     2161 atgacaagtc attcaagaag ttgtagaaac gatgtgcacc ttcgattgat gcacccatta
     2221 atttaccata tcctgtgaca ttcaaaggta agacatggtg tgctgcccaa acgctggctg
     2281 ctgttgcccc tgcttttgaa ccttccaaga tataagcccc aagtaatgct gggatatctg
     2341 cgcctttttc aaatacatat gtcgcaaagt aagagataac gtctctcatg cggatatctt
     2401 tgatgacgat accaccggca gaatatggaa catagcccat tttatgtggg tcaatcgtta
     2461 cagattctgc ttcttcgatt gctttatatg cactgtgtac ttcttctaag atataatctt
     2521 tgttttctgt aaagacattg tatttgtaat gaacatcttt taaatcttca aatggaatga
     2581 agttgttgtc ttcgtctaag aaaatcgcac ggccgtatcc gccataagca gcatctacgt
     2641 gtaagtagaa gtaaataccg tctttttcta atacgcgacg caatgcaacg attttatcga
     2701 taccgtcgat tgctccttct tctgttgatc ctacaacacc gactacacct aggattggtg
     2761 ttttttcagc tgctagacca cgaacgattt tttcaagttc gttgatgtcc atgcgatagt
     2821 tgtggtcaac tggcactgga atcacttggt ctaaaccgat accgatgatg tcggcagctt
     2881 ttagccatga atagtgttta gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt
     2941 ttccgctacg tgctgagtga gctttgattt cgtcgatttt ttctgggacg ctgtctaaca
     3001 gattcatgat ttcttctgtt gacaagttca ttaattccca atcgcttttt ccagcaacta
     3061 gttcaggtgt cacttctttc attgcaagcg gcaatgattt gatgttgcgg gcataccaaa
     3121 gtccttctaa gtttgctaaa gaaccatcag caacgatgtg gccccagcca tctttatagc
     3181 tcattaattt tgcaaattcc attcctactt cttcttccat ttggcttgtt gcaggagaag
     3241 attcataggc aacgttatta ccattccata gcatagcaaa attgtacgct agtaaagatg
     3301 gcatcaatgt ttcagagttc atatgacccc agtaacgtcc ggcattatgc caaggaacgg
     3361 aatgggtacg catccgcgct gaaatttctg atagaacatc ctttgtttta ttcacagtgt
     3421 gttcaaaact agcactgctt ttttcttctg gcgtaataat aggcatgtct tgaggcatgt
     3481 agttttgacg ccagcctaaa tgttcatcca ccaattcatt caacaaagct ttatagattt
     3541 ggccgttctc agctttgtcc ccaatgaata gggcatttag gtttagatct ttcgacaatg
     3601 attcactcat agtgaaaccc tcctaaaaaa atatttaaat tttgtcgagc cctacattac
     3661 acctgttctt ctattctgta aactaagttt tggcctgatt tttaatcata ttttaatatg
     3721 tatcaattat ccgaattgtt cacacttttg aaataattaa aaaaaacgag ggaatttaca
     3781 aatgtaaact ccctcgcaaa gattgtatta acgacaacca gcgttctatt ttaggatggg
     3841 tttcaaaatt ggaatgttgt aatagtattt ccctttttca aggtaaaaac ttactaaatt
     3901 ctaatcaatt actttggcca aaaagtaatt tttcttacct tttcgaataa ccacaaactt
     3961 accatcgaat tcatcggatg gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt
     4021 aatacttata gctccgtttg aaacatcttc acgtgcttga cgtttagatg gttcaatttt
     4081 tgtggaaaca agcagttcca cgatattttc aggtgtgctt gaaatttcaa cgttcggcat
     4141 tttaccgaat ccttgcgcga tttcttccgc atttagttct ttaatatttc cagagaacaa
     4201 tgcttgcgtt attttttgcg cttctttcaa gtcttcttca ctatgcacaa atcttgtaac
     4261 ttcttctgcc aaacggcgtt gtgcttcacg tttctcaggt tctgtctcta cttttttcgc
     4321 taagtcttcg atttcttctt gagacaagaa agtaaagaat ttcaagtatt tcactacatc
     4381 gcgatcatct tgattcaacc agaattggta gaactcaaat ggtgaagttt tcttcggatc
     4441 aagccaaacg gcaccgcctg cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa
     4501 tgggatcgtt aaaccgaacg cttctgcttc tggtccttct aattttcgga ttaggtctaa
     4561 tccagctgtg atatttcccc attggtctgc tccaccgatt tgcaattgca catcgtatgt
     4621 tttatgcaga tgcaaaaagt cgatagattg caggatttgg taagtaaact ctgtaaatga
     4681 gattcctact tccaaacggc tggcaacgat atcttttgct aacatcgtgt tgatgttgaa
     4741 gtttttaccg tagtctctca aaaattcaag taaagagatt tttgacaacc agtcgtagtt
     4801 gttcacaaat gtgatatttg catctttacc aaacaatttt ctcatttgat tggaaagtgc
     4861 gtccacgtta tgttgcactt gttccatcgt ttgcaacaca cgttctgttt tgcggccgct
     4921 tgggtcgccg atcgttcctg ttccgccgcc aatcagaata tatggatgat ggcctgccaa
     4981 ttggaatctt ttcatcatca taaatggaat caaatgtccg atatgcatcg aatcaccagt
     5041 tggatcaacg ccgcaataaa gtgaaatgct cttttcttca acgagctcgc gaagtctttc
     5101 ttcgtttgtt tgctgattaa tggcgtcgcg ccaagttaat tcatcaataa tattcatttt
     5161 actgacctcc gtgtagtttt tttatgaaag caggaacctg ctagcatgtg taattaatta
     5221 tttcttcagg ataggatagg aaataaaaaa agtcccttgg catataagcc aagggacgaa
     5281 atctcgcggt accacccaat ttgtatgtct agatatccaa catacctctc gaacatttaa
     5341 cgctgtcgcg tggcaatcac tgccaaagac tcctgaaatg taattcgctt taaagcatgt
     5401 actggtttgc agcgaccacc agctttctga aacattggct ttaacaacta cttcgtttcg
     5461 atcttcgtct tgttattatt aatatgtgtc tatcatagag cgcaactttt tctttgtcaa
     5521 cagaaaaagg atattttttt taaattcctc attttttctt ttttgaatcc gctctaattc
     5581 cgaacatttt attttcaatt attgttaaaa gaaataactt tttctgtatt ttttctgcaa
     5641 tttatcgaga agtcacttta ctttgcctct tattctttct tcgttacaat aaggaatggg
     5701 acaagtaaaa aaatgaaaaa gcaagggacg aggtatatgg aaaaaaagca aaaaattatt
     5761 ttaggagcca cttctgcttt aactatcgtt attttaattc taagtttgtt ttttgtcatc
     5821 cgatcatcta caaaaaaaga accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa
     5881 gaaccgacag atgataagca acaactagtt actcaagcaa aaaatctggc tcaaagctac
     5941 gattatgacc aagcgattgc gttattggaa aaagatagta agaacggatc tactgaaaca
     6001 caacagcttc ttcaaacact aaaaaaggag aaagaacaac tgatcgactg gtctgatcct
     6061 actcagatct ctcatgtctt tttccacagc ttgatcgtgg acccgcaaaa agccttccac
     6121 tctcaacagg cacaaggata caaagattat atggtgactg ttgaagaatt caaccgttcg
     6181 atcgaacaac tttatcaaaa tgattttgtt ttagttaatt tgaatgattt gatccaaaaa
     6241 gacgaacaag gaaatctttc ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg
     6301 gttctttcac aagatgatgt cagctattat gaatatatgg ataattccgg ttttcctagc
     6361 cgattggtcg tatcaaataa caatgaaatc aaaaatgtct ataaagagaa agataaagaa
     6421 gaaatcggtg actttgacat ggttcctctg atcgatgcct ttgtcaagaa acatcctgat
     6481 ttttcttatc gaggcgcaaa aggtaccttg gccctgacag gttacaacgg agttttaggt
     6541 tatcgaacat caaaatctca atatggagac aatgaaaaaa caaaaaaaga gattaaagaa
     6601 gctaaaaaag tggcggaaca gttgaagaaa gacggctgga gctttgcttc ccatacatgg
     6661 ggtcacttga atatgactca agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg
     6721 cagcaagaag ttgcacctat cctaggaaaa acaaatattc tgatttatcc atttggtgca
     6781 gacatcagcg actggcagcc ttattcagca gaaaatcaaa aatttgctta cttgaagagt
     6841 caaggtttcg atattttctg taatgtcgat gcttctacac cagcttgggg acaattaggt
     6901 acggatttct atcgaaatgc tcggatcaat atcgatggca ttcgttttca atctgattta
     6961 gacggaaaaa atcctattct tgatccattc atcaatgtcc atgatgtcta tgacaaagaa
     7021 gcacgcgaat ctgcttgatt ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt
     7081 atcgtcagtt tcggcttttt tatgctttat ttcttcttcg gtgcgattcc cttgatcagg
     7141 acttctgtcc attctgctgt atatggaagc agttttgcgt atatctccgg cttagaaatt
     7201 ccttctacca ttgcttgaaa atagaggatc aagaactcat ccgaatattt caaatccact
     7261 tttccttctt tccgccctcg atggaaaaga tcaagcatga tactgaaact ttcttgcgta
     7321 tacgcatcca tcgcgtcaga aaattctttt ttttcttttt ttgtatagta tgtcatcaaa
     7381 tcgatataaa attgtttgtt cactcgattg agataacgga ttttgttttc actcatggca
     7441 atcagtgttt cttcaaatgg aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa
     7501 tcattcataa atgcaaaaaa tacttcataa atcagccctt ccttgctgcc aaaatattta
     7561 aaaatcgtcg ctttacttac ctgagcgttc tcagctacct tatccattgt cactccagcg
     7621 gttcctatgt ctgtgttcat caagtcaaaa gctgcttgta aaatctcttt ctttttctct
     7681 tgtgctctct tttcaaaacc gttcatttgt agagcctccc gtaattttaa acttttataa
     7741 tatatatagt tcaaatttat tctgaggttt attataacta tctattg
//
LOCUS       DANKHL010000138         7773 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000138 DANKHL010000000
VERSION     DANKHL010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.143"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            80..2689
                     /gene="clpB"
                     /locus_tag="PW101_002096"
     CDS             80..2689
                     /gene="clpB"
                     /locus_tag="PW101_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDL2514489.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2840..3526)
                     /locus_tag="PW101_002097"
     CDS             complement(2840..3526)
                     /locus_tag="PW101_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514490.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3504..3998)
                     /locus_tag="PW101_002098"
     CDS             complement(3504..3998)
                     /locus_tag="PW101_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL2514491.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4018..5238)
                     /locus_tag="PW101_002099"
     CDS             complement(4018..5238)
                     /locus_tag="PW101_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2514492.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5385..7220
                     /gene="lepA"
                     /locus_tag="PW101_002100"
     CDS             5385..7220
                     /gene="lepA"
                     /locus_tag="PW101_002100"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDL2514493.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            7416..>7773
                     /locus_tag="PW101_002101"
     CDS             7416..>7773
                     /locus_tag="PW101_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2514494.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKK"
BASE COUNT         2604 a         1444 c         1577 g         2148 t
ORIGIN      
        1 ttgaccaaag cgatataata gtttttgtaa agatcaaaga aataagtgat cttaccttta
       61 attaggaggg tatacctgta tgaatattga gaaaatgact acgacgcttc aagaagcgat
      121 tgctgaagct caacagattg cggtcactcg aaaacatcaa gatatcgaca ttgcccatgt
      181 atggaaaatc tttttacaat ctaatcattt tggtcgaaat ttctatacgg atgccggatt
      241 ggatgtcgaa tcttttgaac atgaaattga tcgtctactg gatgagtatc cagttgtttc
      301 tggtggaaac gtccaatatg gacaaaacct cagccagaat ttctttcgat tattgaatga
      361 agcagaccaa atacgtgaaa gcttcggcga cgagtttctt tcaacggaag tcgttatttt
      421 agcgttgatg aaattaaaaa attatccgtt aactgtttat ttgaataaaa acggattatc
      481 tgaaaaagaa ttgcgtaaaa acatcgaaga gatgagagga ggagaaagag tgacctcaca
      541 aaaccaagaa gaacaataca aagcattaga aaaatacggc gttgatttag tacagcaagt
      601 caaaagcgga aaaatggatc caatcatcgg tcgtgatgaa gaaatccgtg atgtcatccg
      661 tattctttca cgaaaaacaa aaaataatcc tgttctgata ggagaacccg gtgtagggaa
      721 aacggcaatc gttgaagggc ttgctcaacg tatcgtacgc aaagatgtgc ctgaaaattt
      781 aaaagataaa acgatttttt cattagatat gggtgcattg atcgctggag caaaattccg
      841 tggtgaattt gaagaaagac taaaagcagt cttgaaagaa gtcaaaaaaa gtgatggacg
      901 tatcctcttg ttcatcgatg aaatccacaa tatcgtaggg gcaggaaaaa cagaaggaag
      961 tatggatgcc ggaaatctgt tgaaaccaat gcttgctcgc ggtgaattac atttgatcgg
     1021 tgccacaact ttagatgagt accgtcaata tatggaaaaa gacaaagcgt tggaacgtcg
     1081 ctttcaaaag gtattagtca aagaaccaac agtggaagat acgatcagta ttctgcgtgg
     1141 attaaaagaa cgttttgaga tccaccatgg tgttaacatc catgacaacg cattagtagc
     1201 agcagctact ttatctgacc gttatattac tgatcggttc ttaccggaca aagcaatcga
     1261 tttgatcgat gaagccagtg caacgattcg ggtagaaatg aactctatgc caacggaatt
     1321 agatcaagtt actagacgtt tgatgcagtt ggaaatcgaa gaagctgcac tgaaaaaaga
     1381 atcagatgat gcaagtaaaa aacgcttgaa aaatttgcaa gaagaattag ctgaacttcg
     1441 tgaagaagca aatgcgatga agatgcagtg ggaaacagaa aaagaagaag tcaattctgt
     1501 ttcagcaaaa cgtgctgaaa tcgataaagc aaaacacgaa ttagaagatg cagaaaataa
     1561 ctatgattta gaacgcgctg ctgtattgcg tcatggtacg atacctcaat tagaaaaaga
     1621 attgaaggaa ttagaagcta aagcaaaaga cagcgagatt aagatggttc aagagtccgt
     1681 taccgaaaat gagattgctc aagtcgttgg cagattaacg ggtatccccg taacgaaact
     1741 tgtagaaggt gaacgagaaa aactaattaa attgaatgaa acacttcata aacgagtgat
     1801 tggtcaagat gaagcagttg atgcggtcag tgacgcggta attcgttcac gggctggatt
     1861 gcaagaccca aatcgtcctc taggttcttt ccttttccta ggaccaactg gtgtcggaaa
     1921 aaccgaatta gcgaaagcgt tagctgagaa cttgtttgac tcagaagatc atatggtacg
     1981 gatcgatatg agtgaatata tggaaaaaca tgctgtgtct cgtctagtcg gagctcctcc
     2041 aggctatgta ggttacgaag aaggtggtca attaacggaa gctgtcagac gaaatcctta
     2101 tacaatcgtc ttgttggatg aaatcgaaaa agcgcatccc gatgtcttca atatcttgtt
     2161 acaagtccta gatgacggac gcttgactga ttcaaaaggg cgagtagtag atttcaaaaa
     2221 tacggttctg atcatgacca gcaatatcgg ttcacagtta ctgctggaag gcgtgactgc
     2281 tgatggaacg attccagaag cagtggctga acaagtcaac acgctattac gtggaaactt
     2341 caaaccagaa ttcttgaacc gaatcgacga cacgatttta tttacaccat tgagcctaga
     2401 caacgtgaaa ggaatcgttg acaaaatggt ggcgcaattg gcacaacgtc tggaacatca
     2461 agaaatcctg ctgacaatca gtgatgaagc caaaacgtgg attgctgaaa atgcttatga
     2521 accagcatat ggtgcaagac cattgaaacg atttatcact aaggaagtag aaacaccact
     2581 agctaaagaa atcgttgcag gacatgttat gcctaaatca aaagtaacga ttactttact
     2641 tgatggacaa cttcatttca aaacagaaga gctagaagaa atcgtctaat cttccattga
     2701 gactataaaa gaaaaaaggg gtctgacaca actagtgtca gacctccttt tttcaaataa
     2761 acagtgttcc agaagtagtt tcttcgaaaa ataagccgaa atttcttgaa gctgcccgct
     2821 tctgtcacaa cctcttttat taggtatttt tcactttttc taatttataa ctttcaaagt
     2881 aacgctcatc ttttttactt tgagaccatg caaacaatcc cattaaggca gcacctatcg
     2941 tatttgtcag caagtcgccc atcgtatcca tcaatgctgc tcgtccgaca aacagtgtgc
     3001 catcagaagc agcaaaacgt tgaagattca tgcctaagaa ctggtcgcat aagaattccc
     3061 agaactccca aaacacgccg caaagtccag caaacgcaaa gccgaataag aggaacaacc
     3121 aaggactggt ttttgatatc tcagcatctt tcatcaagta accaatcaat ccatagccaa
     3181 gagctgtcag gaccattgga ctcaccgcat gtaagatttt gtcccaaaaa gagatgatac
     3241 tgatcaaatg catccctgtg cctaaaaaga cagagatgaa caagaaaaac cagtaaaaca
     3301 aaacgattgc tgctggtatt ttaagtttga aaactttcaa aatgacttgt ggcatgaaaa
     3361 tcaatgcaat ccctaataaa gattccacga taaatgtgat tccttgagaa gtcgaatatt
     3421 tttgtgtgat aaattcataa aaattatagc agacaacgaa tacaccaaaa gcaagtaaag
     3481 ctatttgcac ctttttgatt tctttaatca ctgaaagtcc ctccatctaa aaatcttttt
     3541 acttctgaaa gcaactcaat ttctgaagaa accacttttc cgttcaattt gatcaaacct
     3601 attgtatata agttcaggta gtgaaattga ttttcagctg tttcttgtag tgcagcgatt
     3661 tttttcgggt gggttccgcc ttgctgtcga gtatcagtat ataagccgat cactgggatg
     3721 cctttcgcat aagccacacc gatttccgat gctaccccgg catcgatcgt cagcccatct
     3781 agtaaagcaa tcattaaatc actagccaac actttttctg tgtcagcaag agcaatcatt
     3841 cgactgtctg cataagcagt tttgtcattg attgcttcat tttcttgagg aagatagatc
     3901 gaaagctcag aagagagctc tctgatttct ttaactaact gttggttata taacaaatca
     3961 cttttagcaa acattggtgc ggcaaaataa attttcatct aaacatcatc ctttttatca
     4021 aaaataatcg ctaaagtaag cttccatgtt tggaatgata cgctctagtg acttcagtgt
     4081 ttcttcatct gtttctaacc gttcgattct tgccaaataa gaagcacggc gataattcat
     4141 catcaagcaa gtcaatgttt ggatatccag tcgaacggat tttcctatag gttcatttaa
     4201 gacacggact tgtccctgtt catcccatgt taaagcgaag acaccattat tccaaggagc
     4261 gacaggatct tcaatgatga aatgaaaagc atcagctgtt ccaacaaacg gaaaccgttg
     4321 aaggaattct tttacatcaa cgatccgagc cataaaatat ggttcgatct gctctttgat
     4381 ctgactatct tcaatcaaaa atgctaaagg ctcattttta taaatatctc catgtaccca
     4441 gtagatcatg gaaaaatgtg cggaaataaa gttccataag ccatttcgag cttcttgatc
     4501 aagatagaac atttctttta cgtgaaatat ttcttcagct acccagtaga acagaacacc
     4561 tcttggttca tggttgctat cgtagtagac agcggctgtg cgctcatctt cattttcgaa
     4621 acgccaatac tcttcccaat gaaaatcact gcggaataaa gcaccatggt tttgttgggc
     4681 aaatttgtca taaacggaaa atacgtctgg atgatcaact ggtaatcgct caaccatacc
     4741 acttactggg atttgtttag gtagctgtgt atctctgatt ttaaaggaaa gtttatcaga
     4801 catgatttcc catcctttac gccgatagta ggggatactg taaggataca aataagagat
     4861 ccattgctta ttcttgcgca tattatccaa tgcaacgata attagatctt gcatcaatcc
     4921 atggttagca tattctggat aagttccaac tccagtcaca ccacccattt tataacgtgc
     4981 accgtgaata ttgacttcac aaggatagat agcgatttga gagatcagtt tattttcgtg
     5041 gaaccatccg aacactttcg atacttccaa aatcggtttt ttggaacgga taaaagctcg
     5101 tttattctca aacccgcttt cctcgatgtc tgcttcagtc acttggaaca cataggaaag
     5161 caattcattg aattgatcga tatgttcttg agtgaccggt tttaattcaa gattttctct
     5221 aaaatctttt tgatccattt tttcatctcc atttattagc ttgataacag tatagcaaaa
     5281 ttctacaaaa aaaacttcta tagctgacta attaaaaact ctttaaatta ctgcttttcg
     5341 ttggtataat ggaaagtgct gattatatga aaagaggaga cgctatggat ataaagaaaa
     5401 tgaaagaacg ccaagagcag attcgtaatt tttcgatcat cgctcatatc gaccatggga
     5461 aatctacatt ggccgatcgt attttagaaa tgacgcacac tgtgacgtct cgcgaaatgc
     5521 aagatcagct gttagactcg atggatttgg aacgtgaacg tggaatcacg attaaattaa
     5581 atgctgtaga actcaattac acagcgaaaa atggagaaac atatatcttt cacttgattg
     5641 acaccccagg gcacgtcgac ttcacctatg aagtctctcg aagccttgct gcctgtgagg
     5701 gtgccgtttt agtggtcgat gcagcacaag gaatcgaagc acaaacattg gccaatgttt
     5761 atttagcgtt agacaatgat ttggagatcc tgccagtcat caataaaatc gacctacccg
     5821 cagcagatcc cgaacgtgtc cgtcaagaaa tcgaagacgt aatcggaatc gatgctagtg
     5881 aagctgtatt agccagtgcc aaatcaggga ttgggattga agatattctc gagcagatcg
     5941 tggaatatgt tcctgcacca agtggagact tagatgcacc gctaaaagct ttgatctttg
     6001 actctattta tgatagttac cgcggtgttg ttctaaatgt ccggatcacg gacggtatgg
     6061 tcaaacccgg agacaaaatc aaactgatga gtaatggcaa aacatttgat gttacagagg
     6121 tcggggtctt ctcgccaaaa gcagttgcta gagacttttt gatggtcggt gatgtcggtt
     6181 atatcacagc aagtatcaaa actgtccaag atacacgagt aggggataca gtaacgttag
     6241 cagacaatcc tgccgctgaa gctttaccag gttaccgaaa aatgaatccg atggtttatt
     6301 gtggtcttta tccaattgat acttctcgtt acaacgattt gcgggaagct ttagaaaaac
     6361 tgcaattgaa tgatgctgct ttacaattcg aaccagaaac ctctcaagca ttaggttttg
     6421 ggttccgctg tggattcctc ggacttcttc atatggatgt cgtccaagaa cgcttggaac
     6481 gggaattcaa tctagaattg atcacgactg caccatccgt tatttaccat gttaataaaa
     6541 cggatggtac gactgctaca gtagataacc cggcagattt tcctgaacct gtaacgattc
     6601 aggatgtaga agaaccattc gtcaaagcac agatcatggt cccaaatgat tttgtcggtg
     6661 cagtaatgga actttctcaa cgaaaacggg gagaattcat tacgatggat tatttagatg
     6721 attaccgagt aaatgtcgtc tataatattc cgctttctga aattgtcttt gactttttcg
     6781 acaaattgaa atcaagcacg aaaggctatg cctcattgga ttatgaaatg tctggttatc
     6841 aaaaaagcaa gttagtgaag atggacatct tgctgaatgg agaaaaagtc gatgcattaa
     6901 gctttatcgt tcaccgagac tttgcctatg aacgtggaaa agcaatcgtt gaaaaactga
     6961 aaaaactaat cccacgccaa caatttgagg tgccaatcca agcagctatt ggacaaaaaa
     7021 tcgttgcccg ttcagacatc aaagccttgc gtaaaaacgt cttggctaaa tgttatggtg
     7081 gagatgtttc tcgtaaacgt aaactcttag agaaacaaaa agaagggaag aagcggatga
     7141 aacaaatcgg ctccgtcgaa gttccgcaag aagccttcat ggccgttctg aaaatggatg
     7201 agcaagatag tgtgaaataa cttataatca atcgaaaaac ctattaaata aacctttttg
     7261 agtagatgca acactgagga ttatctgtat aaaagatttt aaactgtcat ttggttatca
     7321 cggcgatact aaaattaaca aaattggaat atttttcttg aagattttta gctgtcagta
     7381 tatgatgaaa gcatctcata ggaggtgctt ttttcatggt tgataattca agtggaaggg
     7441 ttcttaaatc tctaagaaaa gagaaaaaac tatctcaaaa aaagttagct gatttagcag
     7501 gtatttcaca aagtacttta gttaaatatg aaaaagggtc aagaaaaatc cctaaagacg
     7561 tagataatac tctaagcaag attctgaata tagaaactct aataaaagat gaggaagata
     7621 agattgagat tctcatcggt aaactaattg catacagaga tatgaataag ttacttaaca
     7681 aagagttagc tgataatata ggaattagtg aagtattact cagttatgtg ttaaatagaa
     7741 agagaaatcc gtcaaaagaa atgcaaaaaa aat
//
LOCUS       DANKHL010000139         7720 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000139 DANKHL010000000
VERSION     DANKHL010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.144"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..222)
                     /locus_tag="PW101_002102"
     CDS             complement(<1..222)
                     /locus_tag="PW101_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDL2514495.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHD"
     gene            complement(219..737)
                     /gene="msrA"
                     /locus_tag="PW101_002103"
     CDS             complement(219..737)
                     /gene="msrA"
                     /locus_tag="PW101_002103"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2514496.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(757..1470)
                     /locus_tag="PW101_002104"
     CDS             complement(757..1470)
                     /locus_tag="PW101_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HDL2514497.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(1473..2369)
                     /locus_tag="PW101_002105"
     CDS             complement(1473..2369)
                     /locus_tag="PW101_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2514498.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(2527..3369)
                     /locus_tag="PW101_002106"
     CDS             complement(2527..3369)
                     /locus_tag="PW101_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2514499.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            3501..4154
                     /locus_tag="PW101_002107"
     CDS             3501..4154
                     /locus_tag="PW101_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDL2514500.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(4217..4744)
                     /locus_tag="PW101_002108"
     CDS             complement(4217..4744)
                     /locus_tag="PW101_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2514501.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(4764..5711)
                     /locus_tag="PW101_002109"
     CDS             complement(4764..5711)
                     /locus_tag="PW101_002109"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL2514502.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(5723..7609)
                     /locus_tag="PW101_002110"
     CDS             complement(5723..7609)
                     /locus_tag="PW101_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2514503.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
BASE COUNT         2002 a         1582 c         1357 g         2779 t
ORIGIN      
        1 gtcatgatta ttttctaaat atttttccca tgcttcgtca aagatatcca tggacggcaa
       61 atagtcgaca ttcagttcca gataattaga aatttcttcg tagctagagc tgtgcttagg
      121 aaattggatg tctttaccgg cttctgtagc gaaaacagat aaagcatcat gtgtgccacc
      181 gcctcgcagt gtcattaaat aatgaaaaaa gcttcgattc acccttcgtt cctccaattt
      241 tcttcgataa aggcagcccg gcgtgcatgg tttcttttaa aattttcagg atgcttttta
      301 taatactctt gatggtcttc ttctgctaga taaaatggtt gggctggttc gatttttgta
      361 acgattggct gttcgaaacg tccactttca gctaactgtt tcttactttt ttcagcaatg
      421 atccgctgtt cttctgtcaa ataaaagatc accgggcgat aattgtcacc gcgatcctcg
      481 aattgtccga aagcgtcagt tggatcagtt tgctgccagt aaagttctac taattgttca
      541 tatgaaatca atgttggatc atagataatc tccactgctt ctgtatgtcc agtcgtttgg
      601 ctttttactt gttcataagt cggatctggt acatgccctc cagtatatcc tgacatcact
      661 gtatggatac caggaagcgt gtcgaaaggc tggatcatac accagaagca gcctcctgca
      721 aatattgctc gttccattat gattccccct ctaatactac tcatccgatg attttttatt
      781 ttcttttgtc ttgctttcag tactgctggt gctagttttg ttggtatttg agttgtttga
      841 agcatcagag gtatcttttg gcaaatatag actaaaccga atgtcatcat ttactaagtc
      901 gattttatct gctttaatat acatcccgtt ttgcattcgg aattgatcca acctaagcat
      961 gatagtcaga tcatctgtat tgacttcgat ccaatctggt aatttatagt ttctcttgat
     1021 catattcatc acatctttga ttggcaaact caatgttccg atagaaagac ttctagcttt
     1081 tagttggaca tttccgtttt ccatcacata cggatcaaaa tataaataga aatcgatagg
     1141 aaaacctagt acttcgaaag taccattgag tagtgcttca ttttccaaat aaaatttata
     1201 ttcaatatca ctatcttttt gatactcact aaggaaaaag ctgatgattt gattgacttt
     1261 ttctttattt gaattgatcg ttaagacagg atctccttgt ctttccgtaa gagctggtat
     1321 ttctgggagt tctggttccc gattagcaaa aatacggctg cctaaaaata cggtcgaacc
     1381 aagtactacc ccaagaagga taaggaaggc gaatttccag taatttgttt tctttgtttt
     1441 gtttgaagat acgtcagatc ttgttttcat gtttattgat catcctcctt ctctaaccat
     1501 tggttttgtg tctcgatcag ctgatctctc actgcatttg ccatcaattg ataacctaaa
     1561 ttatttggat ggaaatgatc ttcttcatag agcacattat ttttgatgcc atttagattt
     1621 tctgtcgtgc tagttgtttg atctgttcct tcttctgtga cacctacttc ctgattcaat
     1681 ccttgataaa gcaaatcatt gattgggata aagtagcagt tcggcatttt ttttgtcacc
     1741 gtttctgttc ctttgttcca attgttaaca attgtctgca tgtcagtgat ctcaggaaag
     1801 ttcaaataaa atggattgta gatgcctaaa atgtaaatag gtgcggtttt attgagggat
     1861 cgaaattctt ccaatagttc tgtgacattt tcctgatagt ttttcagtgg ctttttaaag
     1921 gaattgaccg ttaacccgaa taaattattt tggataactt tcatcaaatc attaccgcca
     1981 acagtcagtg tgataaaatc agctgttttg atattgtttc gaatttcttc ttttgttttt
     2041 actcgcttta agatctggtc acttcgttca ccatttacac catagttttc tgtttcaacc
     2101 gcggtcaaat tataacggtc ttttagatca tttgcgacaa ggggaacgaa tcctccttgc
     2161 ttggtttgat ctcctactcc ttcagtcagc gaatctccga tagctatgta gtgaagtttt
     2221 tcttttttat ttggatcttc tctcacagta gttactggtt tcattagtgg gccagctttc
     2281 ggtatagaag cagctaaacc tgcatagatg atgatcgcaa ttaataatgg gaacaaaacg
     2341 aggatcaaat gtcgtttcgt taatttcatt ttttcacctc ttgtattcga ttggttctaa
     2401 tgttatcaaa aattttatca aaaaggtagt ctttatgaaa aaagaaaaca attattttcc
     2461 taattttata aagaaaaaga gaataagctg caagcctttc aggctgaggg cttatcctct
     2521 tttcgactaa tctacataat acatgattgc aaacgcgtct ttaccagtat gcgtagcgat
     2581 aaccggattc gtatgcagta cagggatatg catgtccggg aataattctt gcaaaccctc
     2641 tttgaattct tgcgccaatt cttttgcatc cgcatgtgaa ataccgattt gtcgcacatt
     2701 tggcaattct ttcagttcat ttttcaaatt attaaaccat ttatcgaacg ttttctttcc
     2761 tcgcccctta gttacaggga ttaattcaga atgggcgaaa ttcatcacca cgcgtatgtt
     2821 caaaacacta gacagcaaac cagttgttcg gctgattctt ccacctttta ccaagttatc
     2881 tagtgtggat attccgatgt ataattttgt attgtcttgt acttttttga cttcttcaag
     2941 gattttctct actgttttac cttcttttgc taattcagct gctcgaatta cctgaaaaga
     3001 taatccttgg tcagtggtat cactgtcgat aaccgttact ttcgtttttg ttagattact
     3061 ggcttgtctt gctgcttcta ctgttccact caagcctttt gtcatatgga tcgaaatcac
     3121 ttcactgccg tcttctccta gctcatcata catcgagatg aattctccaa ttggtggttg
     3181 gcttgttttg ggcagatttt ttgctttcgc catcatttcc ataaatcttt cgccgtctag
     3241 ttggtcatca tcgggatata ctgttccgtc gatcatgatg gatagcggca tgacacgaat
     3301 gcctaaagca tcgcgtacat ttttttccat cgtacatgaa gaatctgtta cgatttttac
     3361 atttgtcatt attttctctc tctctttctt taacaagatg ccttattcat gttaaactac
     3421 atgtaagaca atttctattt ttagtataac agacaaataa tgatttttca ttaattacac
     3481 ctagtaaagg agccgttttc atggaaaaaa cgaaatttaa tcgaacgtat gaaatattaa
     3541 atgaagtgtt taatgcagtc acccatggaa ttgggactgg attgagtatc gcgggactag
     3601 tggttctttt agtaaaaggt gcccacttgc attcctccct ccatattgtc tcttatgcta
     3661 tctatggatc gatgatgatt cttttatttt taacgtctac tttgtttcat tcattgattt
     3721 ttacaaaagc aaaaaaggtg ttccaagtat tcgatcatag ttcgattttt cttttgattg
     3781 caggaagtta tactcctttt tgtttgctga gtatccgagg atggctaggt tggactttat
     3841 ttgtattaat ctggttgttt gcgattcttg gcattatata taaagcgttg acgttgcata
     3901 aacgggagag agtatcaaaa gtatctacga tcatctatat ttttatgggg tggctctgtg
     3961 tcgtggctgc tgttcctctt tatcactctc ttggaatgac tggtgttatt ttgatggtag
     4021 caggcgggct ttcttacacg ataggcgcat tcttttatag cttgaattct atccgctata
     4081 tgcatgttgt ttggcatttg tttgttatgt taggtgcagg ttttatgtac ttctctgttt
     4141 tattctttac ttaataaaaa aaataaagct atggatggga cagctctaaa taacagctag
     4201 tctatccata gcttttttat atcgtcaatt cgcctgtccc gctttttaca ggattctcat
     4261 tacgctgata cgtttcaaat tgatgcggat aacgattttt ttcatccatt tctccttgac
     4321 tagtattgat cagtgaccaa tcagaccagt caacaggtgg aaaataagca tctccatcga
     4381 atacatgatg gatgactgta cggtataata tcgtacaaaa tggcagaaat tcttgataaa
     4441 cagctgaccc tccagcgatg aaaaagatac cgtctgtttc tttttccaga gcaagcacgt
     4501 cttccacact atgcatgaca tgcacgcctt ctgctttata ggaagaatct ctcgtcaaaa
     4561 caatcgtctg acgattcggc aacggacggc tacccatccc ttcgaatgtt tttcgtccca
     4621 tcactagtgt atttgcttct gtcatttgtt tgaagaattt cagatcattt ggcaaacgcc
     4681 aaggaagacg gtcttctttt ccaatcaatc cgttttcatc ctgtgcccaa attgcagcta
     4741 acattttctc atcctttctg attctaaaca gcgataggag ctttgatcgc cggatgtggg
     4801 tcatatcctt ctaattggat atcttccatc tcaaaatcaa atactgactg cttttcctga
     4861 ttcaaaacta aagtcggaaa tgcgcgtatt tctcggctca attgttcttt catttgttcg
     4921 atatgattga tataaagatg agcatctcct aatgtgtgaa caaattcgcc aaccgctaaa
     4981 cctgtttcat gtgcaattag gtgagtcagc aacgcataac ttgcaatgtt gaacggaacg
     5041 cctagaaaca catccccact gcgttgatac aattggcagc ttagccgtcc ctcttgaaca
     5101 taaaattgaa acatcgtatg acaaggaggc aaagccatgc taggaacatc ttccggattc
     5161 catgcagaaa cgatcaatct tctagaatca ggagtctttt tgattgcttc gatcacttgt
     5221 ttgatttgat cgataaagcc accgtctttt gtttcccagt gacgccattg atagccgtag
     5281 atgtgtccaa gatcgccgta tgtttcagca aaggaatcat cggttaggat cttctcgcaa
     5341 aaaagcttgc tctgcttttt atattcttca ttgaattcag gatctgtcaa acatctatgg
     5401 ccaaaatcag tcatatctgg tccattatag tctggacttt tgatgtagcg ttcaaatgcc
     5461 cattcatccc aaatatggtt tttattttgt aatagatagc ggatattcgt atctcctttt
     5521 aaaaaccaaa gcaattcgct tttgatcaat ccaaaaggta ctcgtttcgt tgttaacaaa
     5581 ggaaaccctt tttgcagatc aaaccgcatt tggtatccga ataagcttaa tgtgcctgtg
     5641 cctgttctgt cttctttacg atgcccttct tccaacaatt ttcttcctaa gtcaagatac
     5701 gcttgttcca tcctttttcc tcctaatctt ctacccattc actcaagtat tcccaccgtg
     5761 ccattttttc ttcaagttgt gcttctgttt cagatacgtc gttttgcaat tcttgtaatc
     5821 gagtgaaatt atctccttga tgattcatct cttcttggag caatgagatt ttttcttcaa
     5881 gttctgcaat ctcatcttca atcgtttccc attctttttg ttccatgtaa gataatttct
     5941 tcttttcagc aggttccttg ctctgtattt ttggtttcgc tttttcagca ggctgctggg
     6001 tctctttttg cttcagtaaa taactggaca tgtttccgta ataaagttct tgttttccgt
     6061 ttccttggaa tactaataac ttatctgcta ccttgtccaa gaaataacgg tcatgggaaa
     6121 cagtgatcac agcacctgga aaacttctaa aataatcttc caaaatcgtc aatgttgcta
     6181 tgtccaaatc attggtcggt tcatccaata ataaaacatt aggttgctga atcagcagtt
     6241 tcaacaaaaa taaccggcgt ttttctccgc ctgaaagttt gcctatcagt gtcccatgca
     6301 taaatcttgg aaataaaaat cgttcaagta attctgctac tccgatttga ctgccatctg
     6361 ctgtttttgc ttcttcagca gcttcttgta agtaagcaat catccgttta tctggtgcca
     6421 tttcttcatt ttcttgtgta taatacgcaa gatgaaccgt ttcaccgata gacattgtac
     6481 cgctatccag gggaattctg ccagctagaa tattaagcaa tgtcgatttt cccgcaccat
     6541 ttttaccagt aatacctagt ctttcccgag attgaatcaa taaatctaaa tgttctagca
     6601 gtgtttggtc atttatacgg taactgccat ctttgatttc cagtactttt ttccctaaac
     6661 gttgtgtcgc taggttcagc tctaaatcat cttcttggtt tacttggtac aagttctcct
     6721 ttagatcttc gaaacggttg atccgtgctt gctgcttggt gccacgtgcc tgtacgccag
     6781 ccctcatcca agctaattcc tgtttataaa gccgcttgcg cttttcttct tgttctactt
     6841 ctacacgatc acgttctgct ttttctaaga cgtaagcttc ataatttcct ttatactcgt
     6901 ataattttcc aaatgaaagt tcgaagattc ggtttgctac tctatctaga aaataacgat
     6961 cgtgggtaac catcaatagt gcgccacggt attgtttcaa atagttttcc aaccactcga
     7021 tcgcttcata atctaaatgg tttgttggtt cgtctagtaa tagtaaatcg ggttcatcaa
     7081 tcaacacttg cgccagacta actcgttttt tctgaccacc tgataaagta ctgatttttt
     7141 tatctaattc actgattcct aatttttgca aaataatctt tgcgttggta tctgtagtcc
     7201 aagcatcttc tttattcatt tgttcttctg ctttagcata acgtttttgc acatcagggt
     7261 cttctccgtt ttcccctagt tcgatcaatg cttcctcata tgctcgaaca gtttggatca
     7321 aggggctgtt tccttgaaag actgcttgga gaacagttaa ttcatcggaa aattcagctg
     7381 cttgagaaag ataaccaata cggtaatcag ttggataaaa cactgtttgc cgatcaccgt
     7441 cgctgctatc gataccggca ataatattta agagacttgt tttacccgtc ccgttcgtcc
     7501 caatcaaccc gatacggtct ttttcgtgga ccaaaaaaga aatctggtca aacaaatctt
     7561 tttcaccata agtttttttg aggtcagtta cttttaattc tttcaacatg ttccctcctt
     7621 atcttggata ttgtatcata aaaatagtga acttcaggaa aatttttttg gtatttgttc
     7681 tttttgtgtt ctcaaaaaaa gaacaggacc gattcgatcc
//
LOCUS       DANKHL010000140         7646 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000140 DANKHL010000000
VERSION     DANKHL010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.145"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            338..895
                     /locus_tag="PW101_002111"
                     /pseudo
     CDS             338..895
                     /locus_tag="PW101_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            953..2992
                     /locus_tag="PW101_002112"
     CDS             953..2992
                     /locus_tag="PW101_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HDL2514504.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3101..4252)
                     /locus_tag="PW101_002113"
     CDS             complement(3101..4252)
                     /locus_tag="PW101_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL2514505.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4406..4936)
                     /locus_tag="PW101_002114"
     CDS             complement(4406..4936)
                     /locus_tag="PW101_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL2514506.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5035..5385)
                     /locus_tag="PW101_002115"
     CDS             complement(5035..5385)
                     /locus_tag="PW101_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HDL2514507.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5542..7071)
                     /gene="ahpF"
                     /locus_tag="PW101_002116"
     CDS             complement(5542..7071)
                     /gene="ahpF"
                     /locus_tag="PW101_002116"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDL2514508.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7081..7644)
                     /gene="ahpC"
                     /locus_tag="PW101_002117"
     CDS             complement(7081..7644)
                     /gene="ahpC"
                     /locus_tag="PW101_002117"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDL2514509.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
BASE COUNT         2610 a         1476 c         1408 g         2152 t
ORIGIN      
        1 atgaactcgt aaatagaata tagtaataac cttgttaggt gaggctccta tacaaacata
       61 ggctgttgcc cagaaacgtc gaaagtcgcc aatgggtaaa acagaaactg ttggattaag
      121 gctttttcca atagctaaga atgttttctt tacgttgtat agtgctaaaa ctcggacgat
      181 gagatgatgc taggtcagaa ttcttgttcg aaaaggtttc tgaccttttc tttatatttt
      241 tgtgactgac ttatttgatg agacaaactc gctcctcacc tgacaagtgg attgctgtgt
      301 gaggaggaga tgcggtgaaa aaagatcgat tggtggaggt cgctcggatt cacttctggt
      361 tgcacaatac aaccagaaaa cacaacaagc aaaactgatc tctatcatga gagattcata
      421 tgtcgatatt ccaggttacg gaatgaataa gataaatgca gcttactctt atgggggagt
      481 tgatttatta aatcaaacat taaaggaaaa tttcaaattt gaagctccct attatgcaag
      541 tatcacattt caagatttta tcgattgcgt taatgaactg tttcctggtg gagtcaagat
      601 cgatgcagaa aaatctctag atttagatgg cgtatatata aacaaaggag agcaagtgat
      661 ggatggtaat actttactcc agtatgctcg atttcgggaa gacaaagaag gagacttcgg
      721 gaggatcaga aggcagcagc aagtgatcaa agctatctct cagcagctga aagacgtaac
      781 ttcaatattc aagctaccca aagcggttgg gaaattactt ggtagtatcc aaacgaacct
      841 tccagaaagt gtgcttctgg attgtggtat ggatttcctc aaagacatat gttagaataa
      901 acaggtataa atagtgaaat aaaggaatga caagcaagag aaggaggaaa aaatgaaaag
      961 aagtgacaag cacggcaaaa atcgaacagg cgcttatatt gccggcgcag tgatcttaat
     1021 agcagctgcg ggtggcggtt atttttacta ccagcactat caagaaaccc aagcagtaga
     1081 agctggagaa aagacagttg agcaatttgt ccaagctttg aacaagggag attataacaa
     1141 agctgcagaa atgacatcaa aaaaggcggc aaataaaagt gcattatctg aaaaagagat
     1201 cttagataaa taccagaata tatacggtgc tgctgatgtc aaaggacttc agatatcgaa
     1261 tctaaaagta gataaaaaag acgattctac ttacagtttt tcatataaag caaagatgaa
     1321 tacctcatta ggtgaattga aagatctttc ttataaagga acattagaca gaaatgatgg
     1381 tcaaaccacg atcaactggc aacccaactt ggtttttcca gaaatggaag gaaatgacaa
     1441 agtaagcctg accacacaag aagcagcaag agggaatatt atagatcgaa atggtgaacc
     1501 attagcaaca acaggcaaac taaaacaatt aggagtcgtt ccaagtaaac ttggagatgg
     1561 aggcgaaaaa acagccaata tcaaagcgat tgcttcctcc ttcgacttaa cagaagatgc
     1621 tatcaatcag gcgatttcac aaagctgggt acaacccgat tactttgtcc cattgaaaat
     1681 cattgatgga gcaacgccag aacttccagc tggagctacc attcaagaag tagacggcag
     1741 atattatcct ttgggtgaag cagctgctca gctgattggt tacgtgggag atatcacagc
     1801 agaagatatt gataagaatc cagaattaag cagtaatgga aaaatcggtc gctcaggttt
     1861 ggagatggct tttgataagg atcttcgtgg gacaacaggt gggaaattaa gcattacaga
     1921 tgcagacggt gtagagaaaa aagttctgat cgaacatgaa gttcaaaatg gcaaagatat
     1981 caaattgaca atcgatgcga aggcacaaaa aatagctttt gacagtctag gaggaaaagc
     2041 tgcatcaact gttgcgacaa cgccaaaaac cggtgatctt cttgcgcttg ctagctctcc
     2101 aagctatgat ccaaacaaaa tgacaaacgg gatctcacaa gaagattaca aagcttatga
     2161 agaaaatcct gaacatccat tcatcagccg atttgcgaca ggttatgctc ctggatctac
     2221 gtttaaaatg attacagcag caatcggtct cgacaacggc actatcgatc caaatgaagt
     2281 gttgacgatc aacgggctta aatggcaaaa agacagttct tggggatctt ttaaaacaac
     2341 gcgtgtcagt agtgatgtat cacaagtaga cttaaaaact gctttgatct attccgataa
     2401 tatatatgcg gcacaagaaa cgttgaaaat gggtgagaaa aaatttcgta caggcttaga
     2461 taaattcatt tttggtgaag accttgattt gccaattagt atgaatccag cacaaatttc
     2521 taatgaagat agctttaact cagatatctt gctagctgat actggatatg gacagggcga
     2581 acttctaatt actcctatcc agcaagcagc aatgtattct gtttttacca acaatggcac
     2641 acttgtctat cctaaattga ttgcagataa agagacaaaa gataagaaga atgtcatcag
     2701 cgaaacagca gtacaaacga tcgtgccaga tctgagagaa gttgtgcaag atgtaaatgg
     2761 tacagcacat tctctttctg ctttagggat tccgttggca gcgaaaactg gtacagcgga
     2821 aatcaaagaa aaacaggatg taaaggggca agagaacagt ttcttgtttg ctttcaatct
     2881 tgataaccaa ggttatatga tggtcagcat gttggaaaat aaagaagatg atgattcagc
     2941 aaccaaacga gcatcggaac tattgcaata cctcaaccaa aattatcaat aaaaaattgc
     3001 tttttaagta cagtaaaagg actgtggcgt tatcgtcaca gtcctttttt caaataaacg
     3061 gttttcacga tgtctctttc acaacttctt tttgatttac tcacacttca taaacttttt
     3121 ttgtaaaata tttgagaaca agtggagaaa taagtgtact taataaaatc acgataacta
     3181 aaggtgaata gtactgtggt tcgatcaatt gactttgctg tccaatttgt aaaatgatca
     3241 aagccatttc tccacgcgaa atcataccag caccgaccat caatgaacta tgttgagaaa
     3301 aaccggcaat ctgagcacca aaataacctc caagcaattt ggtcaaaatc gctacaagcg
     3361 tcaatataga aataaagagt agttgttcac taaagtcaga aaaatcaact tctaatccta
     3421 cactgacaaa aaatactggt ataaatactg catatcctaa agcctctaca ttgtggtata
     3481 cttcttcttt aacctttgtc tgactgacag ctatcccagc aaaaaaagca ccaatgacag
     3541 agctaagccc aacaagatcc gctacgtaag acattcctaa acaaataact agcgatacga
     3601 tgataataga agaatttgcg tacacttttt ctgcaaatga aagtaggaaa ggcgcaatcc
     3661 atttgactaa taagaaaata aaaacaaaat aaattagctg ttcaatcaaa acaagcggta
     3721 aagaagcatt gctagaagct tcccccgtta gaaaagatag gctgaaactt agaactaaaa
     3781 cgactaaaat gtcatctacc acagaagcac ccaaaattgt tgatccttct tttgtattca
     3841 ctacgtttag ttcttttaat acttctactg aaatactgac agaagttgcc gcaagaataa
     3901 tcccaaagaa aatcgcttca tttcccgaaa cttggaatgc tttgcctgtc aaccagccaa
     3961 aaacaaccgg gaagatgatc ccaagcaaag caacgaacat accaggtcgg aaatattttt
     4021 tcaaaagaga taaatcactt tccagaccag ctaaaaacat taataagatc acaccaatct
     4081 ccgaaaagtc atggactaga atatcaggtt tgacccaatt caatccagct tggcctagca
     4141 aaacaccaac aagtaattgt ccaatcactg ctgggatacc taatctccgc gagatatgtg
     4201 agccaatcgt tgttgaaatc aatatcaggc ataaaatacc aataaattcc attctcatca
     4261 ttcctttgta taaaatatag cccgaactta gcataacata cattattgaa aaaataggaa
     4321 aatatactca caaaaaaacc aacaatccaa aagatttttt tggattgttg gtttttatac
     4381 ggtctatttt aataactaaa aatgattaac gtatagtcat ttttcttatt ctgatgaaat
     4441 tgataatctc ctggatgccc atgaaaatca aaataaagcc gaatatttgg aatagaagca
     4501 tcaaactttt gaatggattg aacaataaga cgaatccgcc aatcaacaac agaatactac
     4561 tataaacact ccaactttta gcttttgtat tcatgctgaa gaacaactgg aacaagctgt
     4621 tgatgataac agctaatcca agaagaatcg gcaggataga aacgattgca ggtgcaaaag
     4681 catagacgat cagagctaag atcaggaaaa aaagaccact gaacagaccg atactccaag
     4741 aacctgtttt ccttttaaaa cggtaagcct caaaaagatt caacaaccca agcaaaacaa
     4801 gataggcggt gatcagataa ccgataaaat gaaaaacagc agaagggttt agcacaatcg
     4861 caatcccaga aataatataa acagcagctc ttaataaggc gtatttttga atattttgtt
     4921 taatcgtctc aaacatataa gaaaacctcc tttgacttat tttaacgtga actagtcatt
     4981 ttaccaaatt aaatgttaca aaatgagcga aggagaagaa tatctctgga aaaatcaact
     5041 tctgaggatc ttttccattt ttttgccttt tgctagctca tcgatcattt tatctaaata
     5101 acgaatttct ttcatcaagt tatcctcgat gtcttctact cgtacaccac aaacaacacc
     5161 tgtaatcaag gaacgtgaag gattggggac aggggcttcc gcaaaaaatt gttcgaaagt
     5221 cacttgtttt tgtagttgat attctagttc tgtttgggaa taacccgtca gccaacaaat
     5281 gatttcatct acttcctctt ttatacgatc ttttctctcc gcttttgcta catacatagg
     5341 ataaacgctt gcaaaacttg tcgtaaaaat acgattttgc gccataatca gtcacctttc
     5401 tttttttcaa attatttact acttatagta tataagaaaa agagaagaaa aagcgattat
     5461 tcaaaaaaac aaaaaggatt gtgacaaaat tcttgtcacc atcctttttt ccgctctctg
     5521 actttttaca atcttcatag ctcaagtgcg catcagataa tcgaatgctc ctaaagctgc
     5581 agtagctcct gaacccattg aaacaatgat ttgtttgtat ggactatctg tacaatcacc
     5641 agctgcgaaa acgccgggga tattcgttgc gccatttttc tcggtcacta tttcatttct
     5701 tggattcatt tcgatcgttc catctagcca atccgtatta gggagtaagc cgactagaat
     5761 gaatactcct gaaacagcta gtgtatgcat ctccatcgta cggcgatcga tataggagag
     5821 agattctacc tgattcgtac cgctgatttc tttcgtcgct gcatgtttga taacagtgac
     5881 attcgcaaga gagactagtt tatcctgtaa gatttgatct gcttttaatt ctggtaagaa
     5941 ttctagaaca tatacatgtt ttgctaaccc agccaaatcg attgctgctt caacaccaga
     6001 attaccacca ccgataacag caatctcttt tcctttaaat aaagggccgt cacagtgtgg
     6061 gcaataagca attcctttat ttttaaactc tttttcgcct ggtacattga tagaacgcca
     6121 gtgcgcacct gtcgcgatga tggcggtttt tgctttaagt gatataccgt tagctaagcc
     6181 aatattaata aaatgttgct ctttatttaa ggaaacggcc tgttgattca tcataatgtc
     6241 gatagggtaa ctacgcacat gactttctac ttgctgcatc aattttggac cttctgtgta
     6301 agaggtacca atcatatttt caatccctag tgtttcaacg acttgtccgc cgaaagaatc
     6361 agcgacaagt cctgtacgga ttcctttacg tgctgcatag atagctgcac tactagctgc
     6421 tggtccaccg ccaattacta atacatcata aggttctttt tcaaacaact cttcttcaat
     6481 caaagggcca gtgatttttt caacaatttg ttcgatcgtc atgcgtccgc tactaaattc
     6541 ttctccattt aaaaacacag ttggcactgc catgattttt ttatcatcga tttctttttg
     6601 gaacatccca ccttcaatca tcgtatgagt aatatttgga tttaatacag ctaatatatt
     6661 caatgcctgt acaacgtcgg ggcaattatg acaagtcaag ctaacataac tgtcaaaatg
     6721 caatgtttgt gttatttttt tgattttaag actcaaagat tcactgattt tcggtgcccg
     6781 tccactaatt tgtaacatag ccagtaaaaa agaggaaaat tcatgaccga gggggatacc
     6841 agcaaaaacg attccacttt ctttgccaac tggattgata gaaaaactag gtgtgcgcat
     6901 cgaatctctt ttttcccaag agattttagg agacatttct gctatttcct tcgtaaattc
     6961 agctagtttt tgactatttt cactattatc caaactaaca gaaaaaacaa taggagattc
     7021 aatcagtgct aaatattgtt cgagattttg tttagtttct ttgtctaaca tatccaccac
     7081 ctaaatcttt ccaacaagat caaaacttgg tttcaatgtt tcgccacttt ctttccattt
     7141 tgccggacaa acttcacctg ggtgggtacg tacatattga gcagcgcgaa tcttatcaat
     7201 cagtgcacta gcgtcacgac cgattccatc tgcattgatt tccatagctt gaacgattcc
     7261 gtctggatca ataataaatg ttcctcgctg agctaatcct tgctcttcat ccaaaacatc
     7321 aaaaatacga gaaatctgat gagacggatc accgatcatc acgtattcga ttttaccgat
     7381 agcatctgat gtatcatgcc atgctttgtg agtaaagtgg gtatcggtag agcaagaaaa
     7441 gacttcaaca cctaaagatt tcaatgtact gtattgttct tgtaagtctt ctaattcagt
     7501 aggacaaaca aaagtaaaat cagctggata gaagcaaaga atactccagt tccccataag
     7561 atctttttca ctgacttcta aaaattctcc ttgatgataa gcatgtgcat taaattcttc
     7621 gatttttttc ccaatcaatg acatag
//
LOCUS       DANKHL010000141         7614 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000141 DANKHL010000000
VERSION     DANKHL010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.146"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(45..737)
                     /locus_tag="PW101_002118"
     CDS             complement(45..737)
                     /locus_tag="PW101_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2514510.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1174..1743)
                     /locus_tag="PW101_002119"
     CDS             complement(1174..1743)
                     /locus_tag="PW101_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2514511.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1817..2140)
                     /locus_tag="PW101_002120"
     CDS             complement(1817..2140)
                     /locus_tag="PW101_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514512.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2294..3106)
                     /locus_tag="PW101_002121"
     CDS             complement(2294..3106)
                     /locus_tag="PW101_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514513.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3460..4308
                     /locus_tag="PW101_002122"
     CDS             3460..4308
                     /locus_tag="PW101_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDL2514514.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4453..5394
                     /locus_tag="PW101_002123"
     CDS             4453..5394
                     /locus_tag="PW101_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HDL2514515.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVCLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5384..7555
                     /locus_tag="PW101_002124"
                     /pseudo
     CDS             5384..7555
                     /locus_tag="PW101_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2265 a         1507 c         1375 g         2467 t
ORIGIN      
        1 aatagttcaa cttttggggt tcagttcaaa gaggggggga ttttttatcg ttgtggaata
       61 tttaaatacc aacgcttgtc atgaaaatct tgtgctccac ccttagtatt tccttctgga
      121 tcgtttgtcg cacgcatcat gacgtatact ttcttattag ggaaatcacg catattgaaa
      181 gatacatgat agccaacatt tcctaaagta ttataagctt gatttacgtc tggtctataa
      241 atcccatcag ctcttactct agctagttct tttccagtat tgtagtccat aatgaaaata
      301 tactcgtatt tataattagc aatgtgccat ccagctacat gcaagtttgc gttttcgatt
      361 tctccgaact gatcaatgtg ggcgtaattt gttccatctg tcagcgtaga atttgcagca
      421 cctgctcgag ttggatcaat tactggttta tcctccgaag tagttggatt atcatccgta
      481 aaaccatgag ccaaatcata ggctagtttt tctttactta cacccatttc agaaagataa
      541 ccgtaaggat ctgtatgatc gccccaaata ttttgtgtta cccataaatg cgatttgatt
      601 cctggttggt tataaggagt gtctaatgtt aatggaatac catatttcat tgctgaatct
      661 cttgccaatt caacgtatac tttatagttc ttttcaaaag tttctttatc atgtgtatgt
      721 tgtaactcga tttgcacagg acttttggct cacgaggttg tcctactcta catcttgctg
      781 atagatatgc tgaaaggaaa acaccaacat tatgttttgc tttttcagct agaacgagac
      841 aagcaggaat acctttagaa gatatcaaag gttttttagg tcataaagat gtttccacta
      901 cacaagtcta tgcccatatt tctcccgaag tgaaaaaaag atcaatgaat caactagaaa
      961 actatataga agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc
     1021 actaaaacta tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa
     1081 tctagcgaaa aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag
     1141 cctggcttgc ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt
     1201 gatgatgatt ttatccatca attgagattg actgttttgc tctcctatga tttcagctat
     1261 gattgtccga ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa
     1321 ctcgctaagc atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc
     1381 taccattcgt tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg
     1441 taatgtttgg gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt
     1501 aataggttct tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag
     1561 gacatctagt tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac
     1621 tttttcacga ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc
     1681 catcggcgat cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc
     1741 caatggattt cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat
     1801 cttttttcca aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc
     1861 tttcgatacc aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg
     1921 gaaaaaaatg atcgatgatc actagatctg cataccgagg cggcttattt cgagtacata
     1981 catgtataga gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc
     2041 ttgcttcttg gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat
     2101 ctaaataccc tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt
     2161 gtgcaagtgc ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa
     2221 atttttctaa tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa
     2281 ttacgtaacg aagtcattct tggtaaagta cttttgtcag ctttgatttg ctttcgctct
     2341 caaattcgct attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc
     2401 gatagtcttg tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca
     2461 agcgcaaaat tgctagataa tttttttctt ttattggcaa aatctgctcc tctctaaata
     2521 gcaattcagg aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg
     2581 ccggatcctt tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat
     2641 catctgcaaa aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc
     2701 tttctgaacc aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc
     2761 actttctcaa tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata
     2821 gcagattttt cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag
     2881 gttcgctata tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgaa
     2941 tgggacagtc gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt
     3001 tcagttcttc agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg
     3061 aaaataaatt ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac
     3121 ctgatttcta aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc
     3181 cctttctatt tatttaaata cgcaaatctt cctcattatt ttttatttta ctcaatttaa
     3241 tcgtattctc atttcaaaac gaatgtactt taaaaaaaca taataagatc actttgcttg
     3301 taggatgtat tcccttccat tacaatgaaa taaatagcga ttttatgaga aggtgatgtg
     3361 aaaatggaag aaaaaataga agaatccaaa caaaaaatcg atcagttgat ataagaatta
     3421 gaaaaagcgg tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca
     3481 ggtggtcatg tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca
     3541 ctagcaaaag ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct
     3601 agtgatattc ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg
     3661 ggaccgatct ttaccacgat cttgttagca gatgagatca atcgtacgac tcctcgtact
     3721 cagtcagcac ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac
     3781 cctctagaca atcatttttt tgtcttagct acgcaaaatc caatcgagta cgaaggaaca
     3841 tatcctttgc cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct
     3901 tcttttgatg acgaactttt gctacttttg gataaattcg aaaaagtgtt ggataactta
     3961 aatgaagtat tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt
     4021 gtcagtcccg aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagtcatgct
     4081 gccatccagc ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct
     4141 tatgctttaa tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct
     4201 tacgtgtttt cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaagaaa
     4261 caaatcattc atactcttgt tgcacaaatt cctattcctg tgaggtagat tgaggtagac
     4321 aatggaaaaa atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat
     4381 tgtgcttttt gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac
     4441 tctttatgta tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac
     4501 ttgtacattg cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc
     4561 aaaaaagaga actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt
     4621 tgcagaaaaa aacaagagat tttgattctt acaaataaac cgcaagaagt ccaattgctt
     4681 tggcagccaa aagaaagagg atgccatgaa acattaactg tcatttttac agcctatgat
     4741 agtctaggac ttttcagaaa aagtgcaaaa agagagatct cttttcctgt gactgttctg
     4801 cctgcttttt gtaagattca agcagaaaaa ttacagagag ttctggaaaa aaaacagcag
     4861 ctgaacgcgt accaaagagg tcttgatttt cgggaacatc gcgcttatcg tccaggtgat
     4921 tctttcaacc gaatcgattg gaaattgtct gctcatactc aagagtggtt ataccgtgaa
     4981 tacgattatc aagaagaaga atgttctcct cttttttgct tttggggttc gcccagtcca
     5041 aaatttgaac ctctgctaga cttgtatttt tctttatggc atttgagaaa agaaaagcag
     5101 ccggaacttc tgatattagg caaccgcgcc tttcatggca aagaccccgg tcatacctat
     5161 tttgcttctt tggaagctgg cgatgagaaa gcttttttcg cacacttgaa aaaatatgca
     5221 aaaaaacaaa tcgtcctctt tgttcctgag aattctcctg aagtctcaga agctattgaa
     5281 acattgtcaa aagatcgaac ggtttgtctt gtttatttcg tagaaaaaca actcatggtc
     5341 caagaaaaag acaagatatg ttcattgcca ggaggtgaat ggattgacga ttaaacggga
     5401 aaaatggctg cgtggcgttt actcctttgt gatactaagt gtaggtgctc cgatatttgt
     5461 agtagctaat caattgcaaa acagctacct tcttatccta actattggtg tcctatgcct
     5521 tattgtctgc tgtattccta aaaaatgggt acgtttgctg ctttctttgc cagtaatgat
     5581 aggttgtttg tttttatatt ttccttcgaa gacattttcc atattatgga tcttacagtt
     5641 tttttcgtca gtcacagagg aattcttcca tctatcccaa ggtaaacttt attatttgcc
     5701 agaaaagaca gctttttttc tcataatgtt gctgatctat ttatttatta cattgaccgt
     5761 ggattacgac tattggtacg caccctttct ttctttgatg gcttacttca tgatgcttac
     5821 cgtttttcgg aaaaaagatt tactgtacga aatgattgcg gtagtaactg ttttgttcgt
     5881 gtatctggca gcaaggcaat ttttctccat ccggttgttg tcgggaaaca ctcgattcca
     5941 gtcattgact acagttctgc ttatttgttt cttaactttt agcgctatct tgcctttata
     6001 tcttccgaaa gtacaccaat ctctcgagga aacgagtgaa ccgatccgag aatatcttaa
     6061 tgatgaaggg ctttatgaca cactccatga gtatcaatta accggaagtg caagaacagg
     6121 cttcagtgag aatgatgaac agcttggtgg acctttatat gataatggcg aagttgtttt
     6181 tacagcgaac caaaaaggaa atggctattg gaagatcgaa aataaaaatc attacaccgg
     6241 ttctggatgg atagaagatc aggacgcacc catccaacca atccaagaat tccctgttga
     6301 catcacgagc taccaaaccg ttatagaaga caaagaaaac attacattga ccctaaaaaa
     6361 acaagttttc ttaccttatc cttatggaaa tactaccttg actgattttt cattagctag
     6421 tggacaaacg ctttactatt tgcctgaaag ccagcgattc attcctgtcc cgaatgaatc
     6481 gacacgttct gcaactttac aaacagaaag gttagttttt acgagagaag aattaaaaag
     6541 cagtcagtta aaagctgatg atttagaaaa tcagcagaca aatcttcaat tgccaaaaag
     6601 tttgcctaaa cgtattgagg acttggcggt cgaattgacc aaaaatgaag caactttaat
     6661 tcgtaaagtc gaagctgtct aacagtattt aaaaacaagc ggaaatttcc gctattccaa
     6721 aacggatacc gggcatactc caaaagaaca cgattatgtg gattatttcc tctttgattc
     6781 aaaagtcggc tattgtgaca acttttcaac ttctatggtc gtccttcttc gaacacttgg
     6841 tatcccagct cgctggacaa aaggttttac aaatgggctc agaacaggca cagataatca
     6901 agggaatgag gaatacagca ttctcaatag tcatgctcat tcttggccag aggtatactt
     6961 tcctggattt ggctggcttc catttgaacc aaccccttca tttgccaatc caatgggttc
     7021 acaaccagtt gctgaaacaa ccgaaaccgt ccagcctgct atcgaacaac caaatgaaag
     7081 cgaaaatatg gcagaaacta tttccagtga gagatctgag gtcattccat cagaagaaac
     7141 aacactcaca ccagcttata aaatcgtctt attcagcctt ggagtgtgtt tatccgtctt
     7201 gttgttcttt ctattgttat tcagaaaatc tctgcgctgg acaagttatc ttatctatct
     7261 tcattttttt gcttctacta atacgattgc atatcaaaaa cttcttaacc ctttggaaac
     7321 attggtaccg cgcaaacccg cagaatcttt gcaggaatat agcctgcgaa tagaacaaga
     7381 attcaagcta gatctgcgtt tcattcgatt aactactcaa tatgaagcag aaatatatgg
     7441 acgacaagca gatccggtaa aattcgataa aaaaacctat ttagaaataa cgcatataat
     7501 aagaaaaatg gtatacaaaa aaattctgac aaaagggagc cgcttcaaaa aataagataa
     7561 aatttctgga gaatctactt ctggaatgcc gagaaccagc tgattgagta tcga
//
LOCUS       DANKHL010000142         7408 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000142 DANKHL010000000
VERSION     DANKHL010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.148"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(13..342)
                     /locus_tag="PW101_002125"
     CDS             complement(13..342)
                     /locus_tag="PW101_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2514516.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(332..604)
                     /locus_tag="PW101_002126"
     CDS             complement(332..604)
                     /locus_tag="PW101_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL2514517.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(1055..1858)
                     /locus_tag="PW101_002127"
     CDS             complement(1055..1858)
                     /locus_tag="PW101_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2514518.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(1836..2960)
                     /locus_tag="PW101_002128"
     CDS             complement(1836..2960)
                     /locus_tag="PW101_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2514519.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(2976..3791)
                     /locus_tag="PW101_002129"
     CDS             complement(2976..3791)
                     /locus_tag="PW101_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2514520.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(3781..4653)
                     /locus_tag="PW101_002130"
     CDS             complement(3781..4653)
                     /locus_tag="PW101_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2514521.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(4738..6027)
                     /locus_tag="PW101_002131"
     CDS             complement(4738..6027)
                     /locus_tag="PW101_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2514522.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(6255..6899)
                     /locus_tag="PW101_002132"
                     /pseudo
     CDS             complement(6255..6899)
                     /locus_tag="PW101_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2278 a         1589 c         1136 g         2405 t
ORIGIN      
        1 tttttagtat tattataatt cttccatgcg ttcttccagt cgctgtaaat cgcttggact
       61 taattcccca atttttttcc atttgacctt tttgttgttt ttgggtaatt cgtttagagt
      121 tcctacgtca atccaagatt ctttttttag tcctgcctgt ttccaatccc ttatttcaac
      181 gtatttcagc tttattttat cggatttagt atgatatttt gaagtaattg acagaaacga
      241 aattgtgtcc agtccatttt ttagaatata gacaggccgg cgtttaccgc cctctgcagc
      301 tgtgaaagca acgtagacgg ttgctatatc attagtattc atcttcattg aaccattctt
      361 caattttttc gtcagtgtct agtttttgga caggcaattt ttccgctaat ctttgaatat
      421 tatggattcg tttacttttt tccgacaatt caatagaaaa tgggattttt ccttgctcaa
      481 caatttgctt ataaaaaacg gtgacggctg ttttggggct catgcctagt tcatcaataa
      541 tgctttctgc ttcatttttc aatttttcgt ccatagaaat ttgtaactta gccattttct
      601 ccacctcttt ctgacaaaca cattatatca cggaaagagt tcaaatggaa ttaatacaac
      661 tacttttgaa ctccttgaaa tgcagtgtat ttatctaaat ataaggaaag gatatagtgc
      721 taaatggcct tgatagatag aaaagtttca aaatccgcca cagcgataat tgaagcgaac
      781 actcaacgac acacagaaca ggaaaagact cctaaaagaa ccaaattaac taggacgtgt
      841 ctgaaaactc cacaaagatg tggtatgctc tggtaaaaaa cgaatggcac aaccacgaag
      901 accgatatgg ttcttggaaa actttctcca ccctttttgt aattggaggg atagtggttt
      961 attgatagcg attttccaag ttcttcaagt ggaaccagac attgagcacc acggctaaaa
     1021 agtgaatgaa gtctttattt ttaatcaata atttttaatg attaccagta attggttttt
     1081 tcatgcgccc tatttctatt tgatgtggag ttaaataatg ctgataagta aaaatttttc
     1141 cgtcaattaa actgccacta gtgaatcctg ttctattggt aaaaagagaa tagtctgggt
     1201 attcatctac accaaatgat aaagattcag ccataaactg ttttttacca gcaaattcag
     1261 cactgctacc ttgatggata tgtcctacaa aaatacctaa aatatcactt tcggcaatca
     1321 attcagcaaa accttccgga acttcagtta caatagatgg atcttcccac gacaatggat
     1381 gatgaaccaa taaaatcgtt cccttttcag caggtgtacc caaccactct tgtaattgaa
     1441 tcatctgtcc tatcgatatt tttccttcgt gctgggtatc ttgagcagaa tccaaggaaa
     1501 taattcgtaa cccattttga ttcgtacaat ggatatactc tcccgtttca ttgctttcaa
     1561 cactcattcc ttcaaagaaa acactgcgac gatcatgatt cccacgacaa aaatagtaag
     1621 gtgtggttgg gaaatattga tccaacattt tccgtaagac ttgataatca gaaatccccc
     1681 cttcatgtac taaatcacct gttaatagaa taaaatcgta cactgttaac gagagttctt
     1741 gtaacaattt ttccaatgcc aactgtggat ttttaattgt tggaaatagt tcagcagtaa
     1801 acaagttagt ttgatagttt tctctaaaat ggatatcaga gagatgcaaa aagcgcatgg
     1861 ctattccctc tttcttcaat aaaataatca ggatatccta aattttcttg cgtctgataa
     1921 tcaaaaaaat gcattctttc aggttctatg cggagattga ctgcatcacc aatctgccaa
     1981 gtaggttttt cattaaggac aatcatttct ataccatcga ctttaacggt gtacgcataa
     2041 ctttgaccta atagctcacg atacttcact tgaccgtgta aatcgcttgt ttcttttgaa
     2101 aaggttacgt gttctggtct tatgcctaaa tacaatgagc cactaacctg aagaagttgt
     2161 ttttgccaag ctggattcaa tgttaaaaat tgactggcta cctttaatgt tccttgatca
     2221 taagtaactg gcataatatt catgcctggc gaaccaataa aatttgcagt aaaagtattt
     2281 ttaggtttcg tataaacatt tttcggcgta tcaatcatct gaattctacc ttcattcaat
     2341 aaaacaatcc ggtcagctaa cgtcatcgct tcaatttgat catgagtgac gtagacaaaa
     2401 gtttgtttat aacgttgatg aatttttacc aattcttttc gtgcatccaa tcgtaaacgc
     2461 acatccaaat ttgataacgg ttcatctaat aaaaaataat tactccgttt aaccaccgca
     2521 cgagccaatg ctacacgctg ccgttgccct cctgacaaat ctttaggaag tcgatcttgg
     2581 tatggcgtta aacctaacat cttcaaagct tcttccaaac ggtgctgaat gtcagctgtc
     2641 ttaattttat tcgcccttaa tgcaaaaata atattctcct ttacagtcat gtgaggatat
     2701 aaagcataat tttgaaaaac cattgcgatc tctcgttctc cacaaggtag cttatttgct
     2761 agttgtgtac ctaaaaaaag ctctccactc gtaatttttt ccaaaccagc aatcattctc
     2821 agcaaagtag attttcctga accagatggt cccagtaaca caattcgttc tccagcggcc
     2881 acagctaaat caatttcttt taaaatggta tgttctccat atttttttcc gatatttctt
     2941 aattcaagcg tttctcccat gatttgtcct ccaatttatt taatacctgc agaaacaaaa
     3001 gtcccgataa tatatttttg aaaaattgca tacaagacta acggtacaaa cattgtaatt
     3061 aaactaatcg ccattgctac ggtaaaattt gtcccacctt cagaactaat ataggtctgc
     3121 atcgccaacg gcaaagtata attatctact gtccgtaaaa ttaataccgg ccaaacatac
     3181 tcattccaag aggtaataaa aaaccaaata ccggtagaag taatcgcatg tctagtaaga
     3241 ggaaaaacaa ttttactcat gacggttgtt tgtttgatgt tatccaatgc cgccacatct
     3301 agaaggctca ctggaatttc ccgcattgtt tgcgtcaata aaaaaatgcc catcgcatca
     3361 gcaaattgtg gtattatcac accccaagta gtatcaatta aattaagttt tgcaatcaac
     3421 aaataattag gaatcatcgc aacagtaaaa ggaatgaaca tcgtaccaat gatgataaag
     3481 taccagattt tcttcccttt gattcggcaa taagtaaaac tatatgccgc aaaaaaagct
     3541 acgcctaact taacaaccgt tataattgta gcaataaaaa aagtatttct cgtaattttg
     3601 ataattggaa gattttcgaa caaatagcaa aaattttcca aggtaaacgg ctgtggtatc
     3661 aaactagtaa tcgtctgata ggcatctttc aatggcttaa aagaattggc cataataaac
     3721 aataatggat agataataat gattatgatg aacataaata agagatgtgg caaccagcgt
     3781 ttaattttca tagtaaaccc ccttttccac aaatttaaat attaccccta agaaaacagc
     3841 aaatattaat aaagttaaaa ctgaaattgc tgaagattct ccgactttat acatttcaaa
     3901 agccttttga taagcataat agatcacgtt tgcactacca ttattcggtc caccacgagt
     3961 gatgatctta atcggagtaa acacatactg cagaccttga acaatggtca tcaccaataa
     4021 atatagtcct gtcgaactac tcataggcaa cacaattgta aaaaaaatgc gccagatggg
     4081 tacacgatcc aacttagctg cttcgatcat ctcttgatca attcccacaa ttgctgcaaa
     4141 aatgacaatg aaattataac caaaaacttt ccaagcagta atacctgcaa tcacagcaat
     4201 tacccaacct tgtaaattca tccacatagg cagtcttaaa ccaaaatttc ccaacaagac
     4261 agcaattggt cccgataccg gatttaatat ccatgtaaaa accatccccg ccactaccaa
     4321 agaaatcaca gctggaataa aaaataatgg tttatacaaa ttctgccctt tttctataac
     4381 tattcctaat aaaaaggatg taatatatgg gaacacacag ctcagcaaga caaatagaac
     4441 ggtataggac aaagtattct tcaacacttt atgaaagact ggatcagtca gcacctttac
     4501 atagttggca aagccaacaa attctttagt gggggccacc atattccagt caaaaaaact
     4561 caaatagatc gtataaatca atggataaaa aacaaacacg cccaacaaaa taattgctgg
     4621 cgccagatac aaataaccta catagcgctt catttccgac agcctcctca tccttatgtc
     4681 aaaagcagac gaccttgttt ttcctttctt acaggcgctg tcgtctgctt gctttgctta
     4741 tttgagtaac tcattaattt ccttttgagt agctttcatc gtttcattga cggaattacc
     4801 tgataaaatt ttatcccgca tatccaacaa tttttgttca gcttctaatc cagcatctcc
     4861 aggaaatgaa ctccaactga ccatcttatc catctgatca attgctggta tcatcatctg
     4921 attttcagct aagaaagctt ttaatccatt ttgggcgtct gcaacatctt tacgtggtgg
     4981 taaataacca gtaccttctg tccatttagc taaagattca acactataaa gatattttaa
     5041 gaaatcccaa gttgcttcct ttttcttatc cgtttgtgat gtaacagcta acattgcgcc
     5101 accagcaggt agtttaactt ttttcccttc ccaacttggt gactcaactg ctttagcagt
     5161 aaaagtacta tttttttgaa tgttgtccct ttgagcaata gtggtataga ccatcgcgac
     5221 gttaccatca ataaagcttt gcacaccttg ttcccacgta gtgtgtaatg ccgtctggtc
     5281 ttttaccatc ctttgatata attcataagc agcgatacca tctttagaag caaaggtagc
     5341 tttgccatct tttattacac ttgccccatt actttctaaa attgcttgtt gtgcccaatt
     5401 atcagcaggt tcttgaatat acacgccata ctttccagtt ttttctttta ttatcttaga
     5461 aaaagtttga acttcttccc aagtttttgg accattttca tccaagcccg ctgcttttaa
     5521 tatatcttga tttaaatata aaaccggatt actaatggag taaggaatac ccacttgttt
     5581 accatctttt tcagacagtg ccatgatatt gtctaaaaaa tgatctttaa aataatcagc
     5641 attatccgga tccacttctt ctactaaaga ttgaggatta gtatatttga aattttctgc
     5701 aaaataatct ttaaaagccc atcccacttg tactacgtcc ggtatttctt tggaggcaac
     5761 agaagcttgt aaattttgca tcaagccttt atacatatca ggattatatt ttgctacaac
     5821 cttcactttg tcttgagatt gattgtattt atctactaat tcattcactg tttttccacc
     5881 tagggtatcc gcattaacat gccaatactc tatcgttaca cgaccctccg atttttttgt
     5941 tgtcttggta ttcccactac cacaagctgc caaagagatg acacttaaaa cagacacaac
     6001 ccctataaca agatttttct ttaacataac attaacttcc tcctaatttc tttatggcca
     6061 tttttctaat caagaaatat tatgaactac caatgtaaat ttctagttaa ttttctgtaa
     6121 ataattcgaa atttgtgcat ttcggggtac agggtcactg gacatatcca tcaagctaag
     6181 aataaagccc ccatatggtc atgtatccca tctccaaatc tcttaactcc tctgttttga
     6241 ttataaaata gtttattctg tgctcctttc ttccataaat aggcccatgc acaacgtgag
     6301 gttatattat aaacaccaca gcgcgcaagg tactcaatcg tttctagttc attgactgtc
     6361 catacagaaa atgtcaatcc tagctgctgc cccacttcac gggccaactt agttaaccat
     6421 ccttcatgaa tattgacgat ttgaacgctt tgataggcta atttctgtaa ggtatcgatc
     6481 aaatctgctt cggtaatatc cccaagtttt ttcccttcga actgattttc aatattataa
     6541 aatatatgct ttccccaaat cgcagcccaa gataattgta catagccact aagaacaatt
     6601 tgtggccaga tttgataggt ttgtgccagc gaaaaaacgg ccatttccaa tccctcttct
     6661 tttaaatcac agttaatggt catggttgcc ttctcattgg ccactgcttg aaacacctgc
     6721 tcaagggtta ccgaatctgg gacaacttct tacctgtcat gggctagtat tagcgtacca
     6781 tctgtttgct gacgcacatc tacttcaaaa caaagctggc gatccaaaca aaagcgaaga
     6841 aatgcccagc tattctcctc cgtattgtca ctaccagagt gtgcagtgat catggtcatt
     6901 ttgacccctc ctttaattcc gtgattttct cccacacgtc cactataccg atgaagcgtt
     6961 aatttcttgt taatttcttg taaattccct gcgttttttc agtaaactag ttgtaaatct
     7021 tgcatgctcc ttcctacgaa ctcatgaatc gtcgattttg cccttctagc tagtttcacc
     7081 aattaagtag ttctacattc cccgctttta acctactaca cctttttttg ttttcagcta
     7141 ttccttcttc ttgctgaaag tccttgaggt tctttcttac aggataccat caaggtgttc
     7201 aatggcttat tcccattgaa tgaccgagac ttactatcag atttctgctc gttgaaccaa
     7261 gtctttgata caggatttct tccgtcatct tctcacaaat accaccttgc gtttctctag
     7321 cgattggtcg atgtgcaccc tcccagtgga ctgtcactac ccctttttgc tatgcctagc
     7381 ccacaaaaaa agctgtcctc acagacag
//
LOCUS       DANKHL010000143         7353 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000143 DANKHL010000000
VERSION     DANKHL010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.149"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            133..633
                     /locus_tag="PW101_002133"
     CDS             133..633
                     /locus_tag="PW101_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL2514523.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            730..2577
                     /locus_tag="PW101_002134"
     CDS             730..2577
                     /locus_tag="PW101_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDL2514524.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2580..3476
                     /locus_tag="PW101_002135"
     CDS             2580..3476
                     /locus_tag="PW101_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2514525.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3526..3915
                     /locus_tag="PW101_002136"
     CDS             3526..3915
                     /locus_tag="PW101_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2514526.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3902..6247
                     /locus_tag="PW101_002137"
     CDS             3902..6247
                     /locus_tag="PW101_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2514527.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLENGITSI"
     gene            6340..>7353
                     /locus_tag="PW101_002138"
     CDS             6340..>7353
                     /locus_tag="PW101_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HDL2514528.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRT"
BASE COUNT         2468 a         1112 c         1578 g         2195 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcttactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatacgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cccgtcgatt ttgatgaagt gaaagaacgg
      241 attggattaa atgacgaata tgaagagtac gcaattcatg attatgaact gccttttgag
      301 attgatgagt atacaccaat tgaggaaatt aatcggttgt gtggtttagc ggaagagctt
      361 aaaggaacgc caattggtga ggtcgcttca gagattcaac atgccttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatc atctattatc ccgattgtaa tgacatgagt
      481 gatgtagcgt attacttgat tgtagaagct ggtgatttag gcgaagtacc agaacacttg
      541 aagacgtata ttgattttga tgcgtgtggt cgagacttgg agattggcgg gaattatttg
      601 gttacgagtc gaggggtttt tgaactaatg taaatcattt gttagaattg accagaaatt
      661 tctatcgtac ttttactttt ctatttgata tactgaaata gaaaatattt gttgataggg
      721 gtgttactaa tggttggcaa taagtggtac aaatgtgatc ttcatattca ttcaactgcg
      781 agtcagtgct ttcaggataa aaaggttact gctaaggaat gggtagaaga atgtttaaac
      841 aaaggactcg attgtgttgc attaactgat cacaatacag gggaaaatat tgatgaatat
      901 aagaaagtgg ctatcgaaaa tggcttagat ttttttcctg gagtggaatt aacgtgtgga
      961 gaaaatggaa ctcatttact agtcctattt gataaaagtg catgtacaaa tgatatctct
     1021 gatttcttaa ccaagatggg tataaaaaga gaggattggg ccagcgctgc agcttgctca
     1081 atgaaatcag ttttagaagt tattgatttt gctgagcacg agggcttgct cgttattcct
     1141 gcacatatag atgagtttaa tggaatttct tcagcaaaat ttggaaatct aggaaagata
     1201 tttgaatcag aaaatatata tgcagtacag acggttcaaa aagaattttt tgaaaatcgc
     1261 tcacaaatga ttccctcatc taagcgtgac actatctatg atagtgtgaa tgccagatat
     1321 gatggtagag ttggaaaaga tactttggaa tcttggtata aatcagttgt tgaggcagaa
     1381 aaaaataata tgacatttct tagcttttct gataacccac actcgaaagg aaattcaaaa
     1441 catggacttt ggggaatagg cacaagatat tcctatataa aaatgaagga cgaacctgac
     1501 ttgggatcac ttcgcgatgc tttaatgcta ggcgaaactc gagttcattc tgatttttct
     1561 aatttctcca ttaatgaaaa tgaagtttta ttggaaaaat taagcttttc tgggaccact
     1621 cttagcacaa aagaagttgt agtcgaattc agcgataatt taacttcaat tattggagga
     1681 agaggaacag gcaaatcttg tattacaaga ttcttggtct atgtccttgg aaaggaagca
     1741 gaacttgatc aattttctga aatacaaagt gattatcaga actttgcaca aattgaacat
     1801 aatggctctg gaatattttt gaaggataca attgtgaaac tgaatatttt ctataagggg
     1861 acaaaatatc aaataataag aacacaggat agacattcta tttatgaatt tacacaagag
     1921 aatggtctgg ttgaaaaaga aacagaacgg ttgagaatga tttccgataa ggtgagtata
     1981 tactctcaaa aacaaattta tgaaatttca aaaaatcaaa ctagtatact agaattacta
     2041 gatggatata actctgattt gatttcagag tataaaaatg agattgaaac atgtgtaaac
     2101 gaaataagaa aattaaattg ggatattgtt tctgtgaaaa aagaaataca agataaggcg
     2161 aaggtagagc ttgaaattga agatttaaaa aaacaagtag aaaagctatc tcataagagt
     2221 tataaagatg tttatgatga gtattcaaaa gagacagata tttataggga actgaaacgt
     2281 gatgtggagg cacttaagga aattcccaaa aatctaactg atagtgttga caaaattgac
     2341 tttggcaatg gtataaaagt tacagacgaa attgatgaac accgcggaga attgattaag
     2401 aatttagtga ccaatcgggc taagattcaa acaataatta atgaaatgtc agatgaaatt
     2461 gatagttata gagcagagct gaataaatca gattggaaag ttaactataa tgaggtttca
     2521 aagaggtatc aaagaggtat caaaaactta gtgaagagtt atctcaagat gaattaaaaa
     2581 tgttacaaaa ccttgattct ttatatcaat ctttatcaaa aactgaagag cgattggaag
     2641 aaattattca acaagaaata aagcttaaag attaccaagt tgaggttgac tcagcaatgt
     2701 caagaatgaa taaatcatat caaaatctct attctgagag aagtagttcc tcgcgcgaaa
     2761 ttttttctga tattacagga atagaggcta ctattcaacc aaatagggat ttcaataatt
     2821 atgttaaaga gctcagagct tgtattaata aggaggacac ttttgacagt gagtttggat
     2881 ctcttattga taagctgaca aaaaattcca ttactaatca agatatatat aatgacatac
     2941 ttgatataag agttagtggg gaaagtagta ttttttcaga taaaaaatta atcaattcta
     3001 taagaaaatt gaatgatgca caagttcttg aattacaaac ccttaagcca aatgatttag
     3061 tagttatccg acttgaacta aatggaaaaa aagttagttt atcgaatgct agtgctggtc
     3121 aaaagacatc tgcaattctt tctatgattt tggcgtatgg aaattcacca ctcatattgg
     3181 atcagcctga agatgatttg gatagtcaat taattaataa cttaatcgta agaagtatca
     3241 ttacaaaaaa ggaaaacaga caaattttaa ttgtaactca taatgcaaat atcccagtta
     3301 atggtgattc agaatggttg attagcatgg gagattcaaa agaaatatcg gctgatcagt
     3361 gcgggtcggt agatgaaccc aatatcaagc caagaatatg tactgtgatg gaaggtggcg
     3421 aggatgcttt tacaaataga gcgaaacgct atggttttaa acaaattgta gaataacact
     3481 agagggtgag acatattatt atgtccgccc tttttaggag gaaaaatgaa aaaaataaaa
     3541 agctatacca gtatctgggc agttgaaaaa gtcatttacg caatcaatga ttttcagttg
     3601 ccttttcctg tgacttttaa ccaaatggct tggttcgttt tatctttact gtttgtcatc
     3661 gtatttgccc atgtgcctcc actatctatg atagaggagg tctttttaaa atacttaggc
     3721 ataccggtgg ctgtcacttg gtttatgtcg caaaagacgt ttgatggcaa gaaaccactg
     3781 ggatttttac gatcctttat cagctatcac ttgcgattta aggtgacttt tgcaggaaag
     3841 aaagtcaaag aacagaagaa acgatgggat gaacccagta ctcttgtaag gagtgtgaac
     3901 tatgtatccg attaagtata ttgaaaataa tctcgtcttt aaccaagaag gcgagtgttt
     3961 tgcctactat gaattagtgc cttacaatta ctcctttttg tcacccgaac agaaatttca
     4021 agtgcatgat aactttcgcc aactaattgc gcaaaatcgt gaaggcaaga tccatgcgtt
     4081 acaaattgcc acagaaagta gtattcgcgc cacacaagaa cgatcaaaaa aagaaattac
     4141 cggtagactc aaagaagtag cgaaacaacg aattgatata cagacagatg cattagtatc
     4201 catgattggc gatagtcaga ttgattatcg cttttttatt ggctttaaac taattgcgac
     4261 agatgaagaa gtcaatctaa agagtctcaa gaaatctttc ttttctgggc ttcaagaatt
     4321 tgtctatgga gtgaatcatc atttgatggg cgattttgtt tccttatcca atgaagaaat
     4381 tcgctgctat tctaagcttg aaaaattaat ggaaagcaaa ttggctcgaa gatttaaagt
     4441 caggcgagtc acaccgagtg acctgacgta tttgattgaa catatttatg gcgaaaaagg
     4501 gataccgttt gaagaatacg aatttcagct accaaagaaa aagctaaaat cagaaacact
     4561 ggtgaaacgt tatgacttat tacgtccaaa tcgttgttta attgaagaaa aaccaaggtg
     4621 cttacggatg gaacatgaga atcatgagtc gtacgtagcg tatttaacaa ttaatacaat
     4681 tgtgggggag atggaatttc cttcctcaga acttttctat taccagcaac aacaatttac
     4741 attcccaatc gacacaagta tgaatgtaga gattgtgacg aataaaaagg ctttagctac
     4801 cgttcgtaat aagaaaaaag aactgaaaga tttagacaat catgcttacc aatctgacaa
     4861 tgaaacgaat tccaatgtct tagatgcttt ggattccgtg gatgaattgg aaaccacctt
     4921 ggatcagtca aaagaatcta tgtacaaact gagttatgtg gttcgagtga gtgccgaatc
     4981 agtggatgaa ctaaagcgac gctgtgatga agtgctcgat ttttatgatg ataccaatgt
     5041 aaaactagtc cggccctttg gcgatatgat ggggctacat gaagaattct taccctcaag
     5101 taaacgatat atgaacgact atattcagta tgttacgtct gattttttgg cggggcttgg
     5161 ttttggggct actcagatgc taggagaatt agaagggatt tactttggct acaatgtgga
     5221 tacaggtcgt aatgtttacc tgaaaccggc attggcttca caaggagtca agggttcagt
     5281 aaccaatgcg ctggccgccg ctttcctggg ttctcttggt ggaggtaaat cctttagtaa
     5341 caatctctta gtctactatg ctgttttgtt cggtggtcaa gctgtgattg tcgatccaaa
     5401 aggggagcga ggagggtgga aagaaacctt gccagaaatt gcggatgaaa tcagtatcct
     5461 caacttgacg agtaagtcag aaaatcaagg tctgcttgat ccttatgtga ttatgaagaa
     5521 aaagaaagac tcggaaagtt tagcaattga tattctgact tttttaacag gaatttcaag
     5581 tcgagacggg gagaagtttc ctgtgttacg taaagccatt cgaacggtga ctcaaagtga
     5641 tcaacggggg ttattacaag ttattaacga attgcgaaaa gacgggtcac caatcgcgga
     5701 aaatatcgcc gatcatatcg agtcctttac cgattatgat tttgctcatt tgcttttctc
     5761 agacggctcg attacccaat cgattagttt agataaacag ctaaatatca ttcaagtggc
     5821 ggatttggtt ttaccggatg cagaaacgac ttttgaagaa tataccacca tggaattgct
     5881 tagtgtttcg atgctaatcg tcatttccac ttttgccttg gactttatcc atagtaaccg
     5941 agaaatcttc aaaattgtcg atttggatga ggcttggagt tttcttcaag tggctcaagg
     6001 aaaaacgtta tcgaataagc ttgttcgtgc tggtcgggcc atgaatgctg gggtctattt
     6061 cgtcactcaa aatagcgacg atttattaga tgaacgttta aaaaataata tcgggttgaa
     6121 gtttgctttt cgcagtaccg atattcatga aatcaagaaa accttggaat tctttggttt
     6181 ggatcaggag gatcaaagca atcaaaaacg cctgcgagat ttggaaaacg ggattacgtc
     6241 catataattg tgtaaaagtg aagcatcaaa aaagccattg atctcagtta ggagtacaat
     6301 gttttttgca gaaaacatac tcgaatagga ctgatatcaa tgactcaagt acattttact
     6361 tttgaaagcg aagaaattca agcaattata aacgaaagcg gtgcgaatga taccgcaaaa
     6421 acacttatga ctatcatgtt taatcagctg atggaagaac aacgaaatca atatattcag
     6481 gcaaatgcct atgaacgttc agaagaacgg caatcacaaa gaaatggtta ctacgatcgt
     6541 tcgtttacaa ctcgaattgg tacgttagaa cttcatgttc cacgaaccag agacggtaag
     6601 tttagtccta ctatctttga aagatatcag cgaagcgaaa aagctttgat cgctgccatg
     6661 attgaaatgg tcatttctgg tgtctccact cgcaaagtaa ctaaaacagt tgaactgcta
     6721 acggatggtg cgactgtttc taaatcattc gtttcaaatc tgatgaaaca gttggatcca
     6781 tttgtttttg agtggagaaa ccggagccta gaaggctcgg aatatccatt tttcatgtgt
     6841 gatgccctgt atatgaaagt aagggaaaat catcgtattg tctcaaaagg tgtatatatt
     6901 ggtatcggca ttgattctga tggacgacgt acgattcttg gttttgatgt tcaagacggc
     6961 gaatcggaag ataattggga taccgttttt caatcgttcg ttcaacgagg tttgttcggt
     7021 gtaaaactgg tcatttctga tgcacacaaa gggttagtta aggctgttcg caagaacttt
     7081 ttaggtgcga gctggcaaag atgccaagcc cactttttga gaaatatttt tgataagctg
     7141 ccaaagaaag tttcttctga tgtaaaagac gagttgaaga gtatctttaa agcctctgaa
     7201 cttgaattga cacgtgaaag aaaagagcac ttcttggaaa aatatggttg tgattcaaaa
     7261 ttaagtgctg cttgcgacat tttagaaaac ggctttgaag atgctattca aattttgtct
     7321 tttcctgaaa atatccgccg aaggattcgt aca
//
LOCUS       DANKHL010000144         7332 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000144 DANKHL010000000
VERSION     DANKHL010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.150"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            16..483
                     /locus_tag="PW101_002139"
     CDS             16..483
                     /locus_tag="PW101_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDL2514529.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            490..2982
                     /locus_tag="PW101_002140"
     CDS             490..2982
                     /locus_tag="PW101_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDL2514530.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            3203..4126
                     /locus_tag="PW101_002141"
     CDS             3203..4126
                     /locus_tag="PW101_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL2514531.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            4132..5385
                     /locus_tag="PW101_002142"
     CDS             4132..5385
                     /locus_tag="PW101_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL2514532.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            5519..6865
                     /gene="gor"
                     /locus_tag="PW101_002143"
     CDS             5519..6865
                     /gene="gor"
                     /locus_tag="PW101_002143"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDL2514533.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
BASE COUNT         2548 a         1179 c         1611 g         1994 t
ORIGIN      
        1 aagggaatga ataagatggc acatcagaat acctcggatt taattgaatc atatataaag
       61 aaaattctgg aaaatagtga aatgatcgag attcgaagga ttgaaatggc agacctcttt
      121 aactgtgttc cttcacaaat caactatgtg attaataccc gttttaccat ccaacgtggt
      181 tatctagtag aaagcaaacg tggtggtggt ggttatatac gaattgcaaa agtaaggatt
      241 tcagataaaa aacaattact tgagcagata aatcagttat ttgatgatac aatcagtgag
      301 aaaaatgctt ttgctattat tcaaaaactt tatgaagacc aaataattac aaaaaaagaa
      361 ggaaacttga tgttgagtgc aattgcaaaa aacacattga atttcaatga atatgaagat
      421 catacacgtg ctcggattct ccgagcattt ctggaacgtt taagttatga agatgggaag
      481 tgagattaga tggaagaact ttttactgaa agagcccaag cagtattaga aattgcacaa
      541 gaagaagcaa aacggttgaa acatcaatca gttagttcag aacatgtttt actagcctta
      601 gtggtcgagc aaaatggaat tgctggaaaa gtattacgtg aaatggattt gaatgaaacg
      661 gaaatttatg aagaaattga acatctgatt ggttatggtg gtatccgttc ttaccctaaa
      721 ggagtctttc ttccatattc accaagaatg aaacaagttt ttgctttagc tagcagcgaa
      781 gtgaagaaaa ctggttctca aaaagttgga acagaacata ttttgatgag cttattaaaa
      841 gatgaaagta tcatggctac tcgaatcatg atcaacttag gtatcagcct ttcaaaagcc
      901 cgtcaagttc tgaaacaaaa aatggggcta gctggtgatt ctgctggaaa aggaatgaat
      961 cgtcgtagaa atgtaaatgt acaagataaa cggcaaacac cgcaaggaac acctacgcta
     1021 gattcattag cacgtgattt gacgaaatta gcaaaagaaa acaagctgga cccagttgtt
     1081 ggtcgttcga gagaagtgaa acggcttatc cagattctta gccgacggac aaagaataac
     1141 cctgtactgg tgggtgaacc tggtgtaggc aaaacagcca ttgctgaagg gttagctcaa
     1201 aaaatcattt taggtgaagt gccagaagac atgcaagaaa agcgcctgat gatgcttgat
     1261 atgggatctt tggttgctgg tacgaaatat cgcggtgaat ttgaagatcg aatgaaaaag
     1321 gtcatcgacg aaatctataa tgatggtcaa gtaatcttat tcattgatga attgcatacc
     1381 ttaattggtg caggtggagc agaaggagca attgatgctt cgaatatctt gaaaccggcg
     1441 ttagctcgag gagaattaca aacgatcgga gcaacgaccc tagatgaata tcaaaaatat
     1501 atcgaaaaag attctgcatt agagcgacgt tttgctcgca ttcaagtaga tgagccaact
     1561 cctgaagaag cagaagtgat tcttcaagga ttacgtacaa gatatgaaga gcaccatgga
     1621 gtggagatca cagatgaagc tgtacgagca gcagtcaatc tttctgttcg ttatatcact
     1681 tctcgtcaac tacccgataa agcaattgat ttgatcgatg aatcagcagc gaaagtccgt
     1741 ttggatcaaa ccgatcactt aacaaaatca actgtgatca aactagaaat cgatgaacta
     1801 gtacaagaaa aggaagcagc gatccaaaaa caagactttg aaaatgctgc tcaattacgc
     1861 agacaagaaa aagcactacg taaaaagctg caaaaagttt ctgcaattga agcaaaacag
     1921 gagcaaggtt attctgatcg tgtaacagaa gaagatgttg ctacagttgt atcggagtgg
     1981 actggtgtcc ctctacagca gttggagaaa aaagaaagcg aacgcttgtt agaactggaa
     2041 ggacttctgc atgaacgtgt cgttggtcaa gatgaggctg tcaaagcagt ttcacgtgcg
     2101 atccgccgtg cacgaagcgg attgaaagat cctgatcgtc caattggttc attcatgttc
     2161 cttggaccaa ctggtgtcgg aaaaacagaa ttggccaaag cactttcaga agtcatgttt
     2221 ggaagtgaag atgccctgat ccgtgttgat atgtcagaat ttatggaaaa atacagtaca
     2281 agccgattaa tcgggtctcc tccaggatac gtagggtatg atgaaggtgg acagttgaca
     2341 gaaaaaattc gtcaaaaacc atattcagtg attctcttag atgaagtgga gaaagcacat
     2401 ccagacgttt tcaatttact tctgcaagta ttggatgatg gtcatttaac agattctaaa
     2461 ggtagaaaag tggactttag aaatacaatc atgattatga cttcaaatat cggagcgaca
     2521 caaattcgag aagaaaaaaa tgtcggattc aatgtccaag atgttactaa agatcacaaa
     2581 gccatgcaaa aacgtatctt agaagagtta aagaaagctt tccgacctga attcttgaat
     2641 cgaatcgatg agactgtcgt cttccattca ttgagtaaag atgaaatcca tacaatcgtc
     2701 caaatcatga gcaaagcgat tatcaaacga ttgaaagaac aagatattca agtgaaaatc
     2761 acaccatctg caatcgatgt gatcgggaaa gctggatttg atcctgaata tggtgcacgt
     2821 ccaatccgtc gcgcacttca aaaagaaatt gaagatcgat tgagcgaggc tttgcttggc
     2881 ggagaaattc gtttaggtga ccacgtaaca gtaggtgctt caaaaggtaa gatcacattg
     2941 aatgttcgcg gacctaaaaa ggaaacagta ggttccctat agttacttgg cctgtgtagc
     3001 tgaattttta taatagtaag acttgattaa gaggttgtga caagattttt gtcacaatct
     3061 ctttttatat ttggacagaa tatatcgaac tttattttga ttttttgtga aataattttc
     3121 aaaaatccga ctttaaatag aagaagtatt ggaaagtttt tagagctatg ctactattat
     3181 gaagttgtag gaggagaaaa ttatgatcga actcataacc acagtagaaa ctattgaaca
     3241 agcagaacag ttgcttgctg caggtgcaga tacactatat ttcggtgaag agcaatttgg
     3301 attgcgtctg cctgcttcat ttacacgaga agaacagcga caattggtag aattagcaca
     3361 caagtatggt aaaaaagcga atattgctgt aaatgggatc attcatcctg aaaaaatgaa
     3421 aaatgtacct gaatatttgg cttttttaaa agaaataaac gtagatgtga tcacagttgg
     3481 tgatacaggt attatttata cgatgaaaaa aaatcctgaa ttgcgtatac cgtatattta
     3541 tgatgctgaa acattagtaa cgagcgcacg tcaaatcaat ttctgggcga aaaaaggcgc
     3601 agttggagca gtacttgctc gtgaagttcc ttttgaagag atgaaggcga tggaagaaaa
     3661 actcgatatt cctgtagaaa cccttgtgta tggtgctaca tgcatccatc aatccaaacg
     3721 tccactgcta cagaattact ataactatac gaaacaagac gaacaaaaag atcgtgaaag
     3781 aggtcttttt atctcggaac ctaaaaaaga agagacacat tattctattt atgaagatag
     3841 tcatggcaca catatttttg ccagcaatga tctgaatctc tcgaatgaat tgaatgaact
     3901 ttatcagcat cattactgta catggaaatt ggacggatta tttactccag gcgaaaaatt
     3961 cgtaaacatc actcgactat tcgccgaagc aaaaaaacaa attttagaag gtagctggga
     4021 tcaagaaaaa gcggaaaaaa tgactagcga aatcagtaaa ttgcatcctg aaaaccgtga
     4081 attagataca ggattctttt atattgatcc ttctgctatc aaataaggag tatgatgaaa
     4141 gaaatgacag taaaaagaaa attgaaacga ccagaaatcc tagcgccagc aggaacactg
     4201 gaaaagttaa aaaccgcgat ccattatgga gcagatgcag tatatattgg cgggaatgca
     4261 tatggtttaa gaagccgtgc aggtaatttt acttatgaag aaatggctga aggagttgct
     4321 tttgcaaaag aacatgatgc gaaggtatac gttgctgcta atatggtcac ccatgaagga
     4381 aatgaagatg gagcagggga atttttccgc agtttgcgtg atgtcggtat ttctgcagtg
     4441 attgtttctg atccagcttt gatcgaaatt tgtgcgacag aagccccagg tctgccgatc
     4501 catttatcca ctcaagcttc agcaactaat tttgagacat tggaattctg gaaagaagaa
     4561 gggttggaac gtgtcgtttt agctcgtgaa gtttcaatgg aggaagtggc agagattcgt
     4621 aaaaatacgg ataccgaaat cgaagcattt atccatggtg ccatgtgtat ctcttattct
     4681 ggtcgatgca cattgtccaa ccatatgtcg atgcgggatg caaaccgtgg cggatgttct
     4741 caatcttgtc gctggaagta tggattgttc gatatgcctt ttggtcaaga acgaaacagt
     4801 ttaactgata caggcgaagt ggaagaagaa ttctcgatga gtgcagtgga tatgtcaatg
     4861 atcgaacata ttccagaatt gattgaaaac ggcgtagaca gcttcaagat cgaaggaaga
     4921 atgaaatcga tccattatgt atctacggtg tcaaatgtct ataaagcggc tgtagacagt
     4981 tatatggaag atcctgaaaa ctatgtctgc aagcaagaat ggatcgacga gttatggaaa
     5041 gtagcacaac gagaattagc tacaggtttt tactacaata caccaactga aaacgaacaa
     5101 ttattcggtg aacgtcgtaa aatcccagta tataaatttg tcggtgaagt attatcttat
     5161 gatgaggcaa cacagattgc tacgatacgt cagagaaact tgttttctgt aggagatgaa
     5221 atcgaatttt atggtccagg attcagtcat ttcagtcaaa ccgttgaagt tatgcataat
     5281 gaagaaggcg aatccattga ccgagcacca aatccaatga tgattttaac tatgccagtc
     5341 gcacagccgg tgaaacctgg agatatgatc cgaaaacgta aataaggaaa aagctgatat
     5401 atccctgttt taagcatctt cgcatacaaa attcaagaaa agtgttacac ttgttttctg
     5461 tagaaaaagg atcgttaaat aaaggttttg attgatctct tcaataagga ggaacaaaat
     5521 gcaaatatat gattacatcg taattggtgg cggctctggc ggtattgctt cagcaaacag
     5581 agcagggatg catggcgcaa aagttttgct gattgaagcg aatgaattgg gcggaacatg
     5641 tgtcaacgtc ggttgtgtac ctaaaaaagt tatgtggcaa gcaagtacga ttttggaaac
     5701 aattgaacga gatgcctcaa gttacggtat ccaagcagat ttaaaaaatg ttgatttcgc
     5761 cggtctagta gaaagacgag aaaaatatat tgattttcta cacggtgctt atcaaagagg
     5821 attagatagc aatcatgtcg aggctataaa aggttacgct acttttatcg atagtcaaac
     5881 gatcgaagta aacggtgaaa cctatcgtgc tcctaaaatc ttgatcgcta ctggaggccg
     5941 tgcaagtaaa atgaccattc ctggtggaga atacgcaatt gattcaaatg gattttttga
     6001 attaacagag ttacctaaga gagcaatcgt tttaggtgct ggatacattg cggctgaact
     6061 ttcaggtgta tttcgtggtt taggtagtga agtcatgtgg gcatatcgaa aagaacgacc
     6121 acttagaacg tttgataaaa tgctttcgga caatttgatc caaatgtacc aagaagctgg
     6181 aataaaaact tacgcacatc atattgctaa agaaattact aaaaacaatg acgaatatac
     6241 ggtgctattt gaaaatggtg aaaccttaac tggtgattgt gtactttttg ccggtggacg
     6301 tgtaccgaat accgaaaaat taggtctgga aaatacaaat gtggaattgg acaaaaaagg
     6361 gtttatcaaa gtagataaat accaaaatac aacggatgaa catatttttg ctgttggtga
     6421 tgtgatcggt aaattagatc tgactcctgt tgcaatcgca gctggtagac gtttatcaga
     6481 acgtttattt aatggaaaga aagattctta tcttgactat aaacttgttc cgacagtcgt
     6541 ctttacgcat ccaccaattg caacaattgg tttgacagaa gaagaagcat tggaaaaata
     6601 tggcgaaaat gaattgaaag tttatcgttc acgctttacc ccaatgtact ttgcattaaa
     6661 tgattatcgc caaaaatgcg aaatgaaatt gatctgtgtc ggaaaagaag aaagaatcgt
     6721 cggcttacat gctattggtg ttggtgtcga tgagatgctg caaggttttg ccgtggctat
     6781 caagatgggt gcaactaaag aagactttga caatacagta gccatccatc caacaggagc
     6841 ggaagaattc gtaacgatgc gataatttaa tattaacagt tccaacagct ttttattgaa
     6901 caaaccatgt aaaaccagaa aatcagtata ctgattttct ggttttttgt tttttagtta
     6961 tttatgtaag attgcttcga taattctcaa aacgaaataa gtagggaagt tggagatttt
     7021 gattatcttc gtaactgacg aagagtaaat gaatccattt ggtatgcact cttaagttca
     7081 aaatattggt atcgatgttt aaaagaaata gaaatgtatc gaagaacaga tgtttaaaaa
     7141 agtgtgactt tgtataaata aattagaaga agcaaacgag gatgttaaaa gaataccata
     7201 tagttaccaa accaagaaag ttctcgtcaa gttcgctggt acgaatcaaa tcaagtgaaa
     7261 aaagggactg tgaaaaaaat tgtgtcatag ttagtcactt tttccgaaca aatagtgttc
     7321 aatcagctgt cc
//
LOCUS       DANKHL010000145         7290 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000145 DANKHL010000000
VERSION     DANKHL010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.151"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            145..2820
                     /locus_tag="PW101_002144"
     CDS             145..2820
                     /locus_tag="PW101_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HDL2514534.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGIAKIEATFVEAQKKIWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3002..3241
                     /locus_tag="PW101_002145"
     CDS             3002..3241
                     /locus_tag="PW101_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514535.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLAFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNGRVAKEWYLVHS"
     gene            3257..4630
                     /locus_tag="PW101_002146"
     CDS             3257..4630
                     /locus_tag="PW101_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2514536.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGGKADRPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLAYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEAHEAKVVRHIFQLYLTKKYGYKKLCQRLTQQKFFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKTIFLQAQEIRQSRCTAKKDTYPYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKHKVIQQKKQEAQWLKAFEEGNCSLEELKQQLQALQ
                     KPRSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDHVCRIEVTKEKTVCGIYFRQMTQ
                     NILEGNE"
     gene            4634..6271
                     /locus_tag="PW101_002147"
     CDS             4634..6271
                     /locus_tag="PW101_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2514537.1"
                     /translation="METTPTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMSYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPKPILVKGTHEPIISEEDY
                     QKVQERLVLEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQQGEPIQVKDVQRILTSLDRFLAQSEKKQVKALYRTFIEKITFDPNYKEILEITM
                     KFTPAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6434..>7290
                     /locus_tag="PW101_002148"
     CDS             6434..>7290
                     /locus_tag="PW101_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514538.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYYTLCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLK
                     NGKSDLTHSIKKMNSLRVAKAIVAMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPH
                     EDYKIIMNYYLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFMPLDLSTGRKIRFSEIQY
                     EGIGFTLIDLKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNI
                     NYIDSGSDLL"
BASE COUNT         2558 a         1160 c         1443 g         2129 t
ORIGIN      
        1 tgattttgga aatcacaact tttttctttt gtcaagattt tgagtgggat atatttttgt
       61 agaactgaag atcatgaaat tatgagaggt tggaggtaat gtagtcatac aaataaaaat
      121 tttttagaaa gtgagtggta attaatgaaa atgaatcaca cttcaattca gaagcattta
      181 caactgttaa atacactggg gatttcttat gttaatttga gaactttttc tacattaaac
      241 aagaaaaccg tttctcatat tttttctact gcagattctc aagcaattat taactattta
      301 gaaaaacaga aattgtctga aggtacagta aacataactt ataatcctat taaacaagag
      361 gtgattacac aaaaaaagca ttctgtgaga gacaatgata ttgaaaaaat tcggtttgta
      421 tttattgata ttgaccctaa aaggaaagag ggaagtgcaa cagatagcga aaagaaaaag
      481 gcagaaaatg ttatggaaca agtagaacgt tatttgaaag aaaagagaat agaatcattt
      541 gtaaaagttg atagtgggaa tggctaccat atttttcttc ccataaatga acaaccaaat
      601 aatcacgaga cgattcttac tattcagaat tttcttcaat tacttcaccg aagatttgga
      661 gtagaagatg aagttgatat tgatacttct gtttataatc cttcaagatt atgcaaatta
      721 gttggtacac cagcggtcaa aggaaagtcc acaaaagagc gtccgcatcg tttatctagt
      781 ttcctttcta ttcccgataa catagataga agtaattgtt ttaaaattct tgaacagata
      841 gttgaagaaa attccatgat gtcaacctct agagtaaaaa gccatccatc ttttagagaa
      901 gtgttacctt ttattcaagc agactataaa caatggttag ctcactatcc tgaattaact
      961 tataccataa aagagggcga tcataaagga gttaccttac ttatatttga cgaatgtcca
     1021 ttaagaaaac acacgaataa ttctaatggt tcttgtttgt ctgttacggc tgataataag
     1081 attcgatttc attgtctgca tgcttcggat aaaaataaag atattcaaga ctttgtaaaa
     1141 aaatatccgt tgccaaaacc tgtaaatcct tctgtagaat cattagaatc caatcaagaa
     1201 tattattttg accagtttaa actgaatcaa gaaggagtat ttttggtgaa agaaggtcaa
     1261 gaagaaaaag ttgcttgtcc gatgtttatt aagcaaatac gcaaaaatat cgaacaaaaa
     1321 gtcattacac ttgttcttgc ctattatgtc gatcattgtt gggatgaaat agaaatagaa
     1381 ggtggatgtt tacaaactaa taattttaaa caattagcaa aacatggctt aatcattaaa
     1441 gctagaagag aagctgaagt cgttgacttt ttacagattc aacgggagaa tgctccaatc
     1501 ttttttgaac atgaatctct tggctggaaa aaagaaaatg aagcaatgat ttttcagtta
     1561 caagattctt ttggacgtga aattccttct gtattaacta aaaactcatg ctaccaatta
     1621 acaactaatg gtacagaagc ggaatttgac gcattggttc ataagtatgt tttaggaacg
     1681 aacttagaac ttgcttggtc gattggtatt gcttctattg tgttaggcta tttaaacgtt
     1741 acgaaggcta atactttttt gagtttacta attaacctac agggcaagag cacaacgggt
     1801 aaaaccacta cgttacactt tattgcctct ttatttggaa atccaactac ggttcttaga
     1861 aatatgaatg ctaccaataa agccattata aagctagcat caaacaatca gggaggtgtt
     1921 ccactaatac tagatgagtt aggagcagca actcaaaata actttagctc gttgatctat
     1981 caattagctt caggagaaga aaggctaaga ttagattcta attgtcagct tatgccacaa
     2041 gagacttttt ctgttttgac ccttacctct tctgaagagc gtttggaaaa ttatttcaat
     2101 gagaaattaa ctggtttgaa agttcgatat atcgaatttt ctgatatcaa gtttacaaag
     2161 gatgcgaatc acgcagagaa agttaaaagt ctttcttcta atacttttgg ttggggtata
     2221 aagttatttc ttttgaaact ctttgaacaa gggattgcaa aaattgaagc aacttttgtc
     2281 gaagctcaaa aaaaaatatg ggctgtttta cctgaacatt ctttgaagca acgcattggt
     2341 aataatctag ctttgattta tagtggagct ctcctaattc aagatatact gggctatcct
     2401 ttacgattag agcatataca gacggtgctt attaattcgt acgaacagtt aattgtagat
     2461 actagtttag gaagtgagca aacggtattt gaccgagtga aaacgttgtt ggtgatgaat
     2521 agccataaat ttttaacaaa cagtgacaac aaacatacgc ccagtacgat ttggggaaaa
     2581 gtatcaattc aaaaaaatgg ggatattaag gtaaatattt ttcctatccc gtttgaggac
     2641 ttgttaaggc gagaatttca agtaaaggat ctaacctatc tttttagaca attgcttgat
     2701 tcgggtaata tgtgtgcaga aaaagggaga agaacgaaac gtattcacat gaatcgtcaa
     2761 gttcttccaa catacgagat tctattacct tcttcactca aatcgtattt tcgattgtaa
     2821 tttagaataa accaaagtat gaaagtgagg caccatgtta tggaaatatt tcttattgat
     2881 ttagcaaaac aactgattac ttatctgtat gatgacgagc aaacccaaac ggaagtaaat
     2941 gaagcttgat ctatgcttca tttacttcat tcaatcaaac atttaagaaa ggggaattct
     3001 aatgactaaa aataaaatga cactaaaagc agaggttctt ctgtatattc aagaacattt
     3061 ttctaatcaa gcctttttta caaaaccaat ttacttggct ttcgaaataa gaggggtatc
     3121 tgctggatct attggaggta ctcttcaagc gttgaagaac gaaggatatt tagaaaatca
     3181 ctttgtgcaa cgttcgttca atggaagagt cgcgaaagaa tggtatttag ttcattcata
     3241 gagaggtgat tcaagcatgc gtgtggcttg ttatgatcga acgtctgttt atgaagaagc
     3301 gaaaatcagt aatgacgctc aatatgatgc agtgaaagag tatgttttag agaaatatga
     3361 acaaattcct caacgatttt cagacaaagg agaatcaggt ggaaaagcgg atcgtccagc
     3421 ttttcgacag cttagagctt gggtttgtgc gtcagaagaa gagggggtga tctatgtctg
     3481 gcgttatgat cgattggcac gtcaagccaa aatagcctta gaatttattg agttgtgcca
     3541 acaacatcag gttcaagttt attcgatttc tgaacctctt cctgcttctt tctcgcacgc
     3601 gtcaacgaat aaactatttg tgcaattatt gttcactttt gcagaacaac aacgaaacgt
     3661 cattattgaa aatgttcgtg ccggacttgc ctataaaaag caacaacggc aatatttgtc
     3721 ttctgcggtt ccatatgggt atcgtttagt gaatgggaaa atccaacaag aagcacatga
     3781 agcaaaagtt gtccgccata tctttcaact ttatttgact aaaaaatatg gctacaaaaa
     3841 gctttgtcaa cgattgacgc aacaaaagtt cttcttccgt gaacgtcctt ttcagcccta
     3901 tcatatttat agtatcctaa aaaatccatt gtactatggc gaaatcaaag gaggatcttt
     3961 ggggaagtat ctaggaacct ttgaaccgat tctctcgaaa acaatctttt tacaagccca
     4021 agagattcgc caatcaaggt gtacagctaa aaaagatacg tatccgtact tattacgtca
     4081 aaaaattcgg tgtcccttct gtggtcgtca tctgagttcg aagtatcaat ggaatacaaa
     4141 gaagacgaag acgcttcatt attatcattg taccgaccga agctgccaag gaatctttct
     4201 tcgggcagaa agggttgagt cagaagtatt ggaaaagata caggcttttt ttcaatacga
     4261 aggcgtctat caagccttga ttgcccaatt gacgcaacaa ttagccgaaa agcgaaaaga
     4321 tgaaaaacac aaagtaatcc aacaaaagaa acaagaagca caatggctca aggcatttga
     4381 agagggcaat tgttcactgg aagaactcaa gcaacagctg caagctcttc aaaagccgag
     4441 gtctacttct tcgtttcttt cactccaaca gtgtgaagca aaagtgaaag aatggcttca
     4501 actaaaagac ctctctagtc gacaattgtt atgtgaccat gtttgtcgga tcgaggtaac
     4561 aaaggaaaaa acagtctgtg ggatttactt ccgacaaatg acacaaaata ttttagaagg
     4621 gaacgaatga tctatggaaa cgacaccaac caaacgagtc gcattgtata ttcgggtgag
     4681 tacccaagaa caagctttag aaggacatag tattcaagca caagaacaaa atggacgaca
     4741 gtttgccgaa cggatgagct atgaagtggt agaagtttat gccgatgaag ggattagtgg
     4801 gaaatccaca aaaaatcggt tcgcctatca acgaatgatg aacgatgctc ggaaccatcg
     4861 ttttgatcta ctagtgattt ggaaattgac ccgtctcgga cgcaatatgt tagatgtctt
     4921 acaaaccgtc gaggaactgt tgcgatatga tgtggggtta cattcgatct cggaacaatt
     4981 tgacgtgact acttcgacag gaaaactaat gttacaactc cttgggagtt tcggtgaatt
     5041 tgaacgcaac caaattgcgg agaatgttca gatgtcaatg caaagcttag tgaaagataa
     5101 aaagcgttat gcaggtggac gacgtttagg ctatgtcagt ggtattgaca cgaatggtca
     5161 aaaacaattg gtcattgaac cagaagaggc gaaaattgtt cgtctgatct atgcaaaata
     5221 tttaggcgga gcagggtatc gagcgattgc caattttttg aatcgtcaag gatatcaaac
     5281 agtcaaaaag aatgcttttt ctaccacggc agtcaaagat atcttacgca ataaaattta
     5341 tgcagggtat ttagaatatg cacgctatat caattgggaa accaaacgac gaaagggcaa
     5401 gaacccaaaa ccgattttag tgaaaggtac ccatgaaccg attatttcgg aagaagatta
     5461 tcaaaaagtg caagaacgcc ttgttttaga aggcaaacag cccaaatgga accatacagg
     5521 tgaaaatgta ttgacaggct tgttacgttg tccagaatgt ggggcaccga tggcagcgag
     5581 taatgtcaca aatactctaa aggatggcac aaagaaacga attcggtatt attcttgttc
     5641 agtctttcgg aataaaggag caagtgtgtg tcatgccaat tcgattcgtg ccgaacaagc
     5701 ggaaacattt gtcgccgacc gattaaagga gatcgttcag ctaccagaag ttcttccccg
     5761 attggtggca gcgttaaatg aagaaatagt gcgacaaagc caaccattgg aacaagaact
     5821 cgtggtgtta ctggaacgaa aggaagaatt gaagactaaa attgaaaaat gggaagctgc
     5881 tttggaagat agtcctgaat tgtttccgat gttaaaagac cgcttagacg aattgactga
     5941 aaaacgacga cagttgcaca ttcgagaaaa tgaaatctta gggatctttc aacaacaagg
     6001 agagccgatt caagtcaaag atgtccaacg gatattgact agtttggatc gttttcttgc
     6061 ccagagtgag aagaagcaag tcaaagcctt gtatcgtacc tttattgaga aaataacctt
     6121 tgatcctaac tataaggaga tattggagat tacaatgaaa ttcacgcctg ccgtggtaca
     6181 acaattgaat gagcaatatc aaatagccgt atccaaaaca aaggatacgg ctatttttgt
     6241 gttaaagaca ccctttacgt taacgatcta gtctaggtta tatcaaacaa cgttagaggg
     6301 gcgctaacgc ttctctagcg atttgaaatc gttagatgag tcgttaggga ggtccttatc
     6361 ataaataggg atatttatta atgaatattt gaaatataat tgtatataag aattaaaaag
     6421 agtggggata gatatgaaaa agatgggtaa gtgtatttta tgtgaaaaat acactgaact
     6481 tactaaagaa catgtaccac ctaaaaaatc agggaatact ggaggtaaaa agaccagaac
     6541 tggtaattta gatgatttcc ttaagagcga ttttacaaaa ggtgattttc cgaaggggat
     6601 taaaaggaaa cctcaaggaa atgtttatta tacgttatgt agtaaatgta atagtttttt
     6661 tggtagtgag tatgttgagg aatatattag atttgcagaa gacaataaaa atttccttta
     6721 taacaataca agtttaaaga atggtaagtc tgatttaaca cattcaataa agaaaatgaa
     6781 ttcacttaga gttgcaaaag caattgtagc aatgtttttt agtttaaatg gggatgaaga
     6841 ttcaatggat aagccattct tagattcggt acgagaatat cttagcaatc ctaaaagtac
     6901 tttatttcct catgaagact acaaaattat aatgaattat tatcttccaa taggtgctcc
     6961 acagaattct aaaatcaaaa ttcctaatga cattgtaaat gatggtgaca ctcacaatgt
     7021 aataaaaggt tttatgcccc ttgatctatc aactgggaga aaaattcgat tttcagaaat
     7081 tcagtatgaa ggaattggtt ttactcttat agatttaaaa aattcaactt ttgaaataaa
     7141 agaaggatta gatttaaaaa atctcctttg tgcagatgat aaaatcaaaa aaatacaatt
     7201 gtacaaagtc cctatattaa caccaacagt acttacaatg ttggagatgg aaaataatat
     7261 taattatatt gactcaggtt cagatttgct
//
LOCUS       DANKHL010000146         7275 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000146 DANKHL010000000
VERSION     DANKHL010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.152"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..609
                     /locus_tag="PW101_002149"
     CDS             <1..609
                     /locus_tag="PW101_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2514539.1"
                     /translation="NKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITG
                     HGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKW
                     QQIKHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNG
                     LTSTGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            621..1541
                     /locus_tag="PW101_002150"
     CDS             621..1541
                     /locus_tag="PW101_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2514540.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1582..3039
                     /locus_tag="PW101_002151"
     CDS             1582..3039
                     /locus_tag="PW101_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2514541.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3384..3998
                     /locus_tag="PW101_002152"
     CDS             3384..3998
                     /locus_tag="PW101_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2514542.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            3991..5715
                     /locus_tag="PW101_002153"
     CDS             3991..5715
                     /locus_tag="PW101_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2514543.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5712..7163
                     /locus_tag="PW101_002154"
     CDS             5712..7163
                     /locus_tag="PW101_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2514544.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2489 a         1211 c         1453 g         2122 t
ORIGIN      
        1 aataaacgac tcgttaaagt ttatcaaacg atgtccttac taccttattt tctatcttgg
       61 gtcattatca gttactttgt ttacgctttt ttaagtcctg acaaaggaat attcaatcag
      121 tggatcactg gacatggagg agaagctgtc aattggtata atgaaccgaa atattggccg
      181 tttatcttgg tgttcattgg tacatggaaa gggattggtt ataacagcat tatttacttt
      241 gcatctgtta tgggaatcga tccgacttat tatgaagctg ctatggttga tggagctagc
      301 aaatggcagc agatcaaaca tgtgacgata ccacaactta ttccactaat gacgatttta
      361 acgattttag ccgtaggaaa cattttccga gctgattttg gcctgtttta taacattcct
      421 aggaattccg gtgcattata tgatgtgact caagttttgg atacgtatat ctacaatggc
      481 ctgacatcca caggagattt tggtatgacg gctgctgcag gactttacca atcagttgtc
      541 ggctttgtct tgttgatgat cacaaatacg atcgcccgac gttttgatag cgattctgca
      601 ttattctaag gaggaaaaaa atgaaaaaaa agaaagtgga gagagtccaa gtccgctctt
      661 tcaataagac ggctaatttt ttctctaata taatgattgc actctttgct atttcctgta
      721 ttttgccatt tatatttgtt atcgccatct cttttaccga tgagtccata ttgtcgactc
      781 acgggtacag tttttggcca gaaaagttca gtatatttgg ttatacgtac ttgttcgacc
      841 agatgcagga taaaattttc caagcgcttt tcgtcactgt acttgttact gttttaggta
      901 cactgatcaa tagcacggct acttctttat atgcttatgc gatttcacgg tcaaactttc
      961 cattccgtcg tttttttacc gttttttgtt tgattacgat gttgttctca ccagggatgg
     1021 tggccaatta tcttgttatg acaaatcttc ttcagttaaa agatacgatt tgggcattga
     1081 tcttgccaat ggctgtcagt cccttcaata ttattgtgat gcgcacattc tttaaacgtt
     1141 cggtctctga ttcgattatt gaatcggcac ggatcgatgg agctagcgaa ctgcgaatat
     1201 ttgttcagat cgttttaccg ttagctgttc caggtattgc gacaatcagt ttatttgccg
     1261 cattgggtta ctggaatgac tggttcaatg ctttactata catccaagat gataagctag
     1321 ttccactaca gtatcttttg atgaaaatcc aaagtaatat tcaatatctg acacaaaatt
     1381 caggagcagg aagccaattg acaggcggat tagcctcagt tccaggagaa tctgccagaa
     1441 tggcgatcgt agtcatctcg acgttgccta ttgcgatcag ctatccattt ttccagaagc
     1501 attttgtcaa aggtctaaca atcggcggag taaaagaata aagaaacaaa aatataaatc
     1561 ataaaattag gaggaagaaa aatgaagtta tggaagaaac tagcctttac aggtgtttca
     1621 gcgttgttgt tgggaacatt agcagcatgc ggaggttctg gttctaaaga ccaggcagaa
     1681 gctagcaatt cggacacttt gcagatgtat caaatcggag ataaaccaga taatttcgat
     1741 caattgatgg aagtagcaaa caaacgaatc gaaaaagaga tcggtgtaaa agtcaatatc
     1801 aattacattg gctggggcga ttacgaaaag aaaatgaacg tcatcatttc ttccggggaa
     1861 aattatgata tcgcctttgc gaacaactat gtttccaatg cccaaaaagg agcctttgct
     1921 gatttgactg aattagcgcc taagtacgct gaaaaagctt ataatgattt agatgaagca
     1981 tatattaaag gaaatctggt caatggaaaa ttatatgctt tcccagtcaa tggaaatgtc
     2041 tttgctcaac aagtacttac ttttaacaaa gctttactag ataaatacga tctttcaatt
     2101 gatggaatcc aatcttacgc tgatgcagaa aaagtcttga aggaattcca tgaaaaagaa
     2161 ccaaacacgg cagcttttgc cattggacaa ggcttcaaag tccaagggaa ctttgattat
     2221 cctttaggga atacgttacc atttgctatt gatctggatg gagatgattc gaaaatcatc
     2281 aaccaatacg ataatgaaac gtatatcgga ctgttacgta caatgcatag ctggtacaaa
     2341 caaggactca ttccaagtga cgctgcaacg agcaataagg attatccact agaaggaaat
     2401 acttggctaa tgcgtggaga aacacaagga ccgtatgatt atggcgatac aattttgaca
     2461 aatgcagcaa aacaagaact tgtctcaaaa gcaattactg ttccactaaa atctacagct
     2521 caagcacaaa tggcaaattt tgtcgtatca aatacttcaa aaaataaaga aaaatcagta
     2581 gaacttctag ggctgcttaa cagtgatcca gaattattga atgggcttgt ttgggggatc
     2641 gagggagaag catgggaaaa agtcgatggt tcagatgata aaatcaaatt gctagatggt
     2701 tatcagccaa atacacatat gtctgcttgg aatacaggaa acaacaagat tctatataca
     2761 caagagtcta ttacggatga catgattgcc aaacgtgatc aatcgattgc tgatgcagaa
     2821 acttcaccga ttttaggatt cagcttcaac actgacagtg tcaaaacaga attaagtaat
     2881 atttctaatg tcatgaatca atatttagat ggattgaata cagggactgt tgatccagat
     2941 gaaacattac cgaaattgaa agacgctttg gacaaggctg gctatgataa ggtattgaaa
     3001 gaaatgcaaa aacaatatga tgagttccga caagaataat aatgaataaa gacaaagggg
     3061 ttgtgacaaa agacgtgtca caacctcttg ttccgaataa acggtgttca aaagccagtt
     3121 tctcggtatt aagctaaata tatgtaaaaa tgtggaaagc catattcaca aaatttttct
     3181 tactactcga aactaagcgg ctatttcaca aactcttggt aaacggtgtt caatcagcta
     3241 accctcggta ttcgctcaca aaacgacaaa atatgaggaa ctatttttcc gtttgtttct
     3301 ctaataatca ggtcaaggtg ctgattgccc aacctttttt tgtataatga gaaagtataa
     3361 acttaatgtt tggaggagaa gatatgttag ggaaggtttt ggaagagtta tttatccgga
     3421 tctgggtcat tataaaacta accttgtatt tctggatata tacatttgcc ggcgggatca
     3481 tctttggatt aggtgcggca tggaaaacag tcaatgaact attttatttg tacggattcg
     3541 agtataaaga aatcacaata aaaagaggct ggaatatata taaaagaaat tttttaagag
     3601 gcaatctgct atttagttta tttttatctg gtactgcttt attgagttac aatttatatt
     3661 tatctgttca aatcaaaggg ctcatttttt tagctattga ttttatttta ctttttgcct
     3721 tgctctgcct atttgctact tatcaataca gtatgatttt agatagtgaa tatacgatct
     3781 ctgttcctaa tctgctaaag ctaagtttta tttcggtttt ttccagcttt tcgtcttttc
     3841 taaaaataat tattggtagc ggtataattt taggcgtgac ttggcaattc aaaggattga
     3901 tcctgtttgg tgtcattggt cttttgacgg tttggaatgg tacgatgaca acacattggc
     3961 gtgaagaatt agataaacag ttggaaagtt atgaataagt ttcggattct attttcaaaa
     4021 ggaaccttac taaatcgtct gttgcggaaa tacgcggtta tcatgatcgg tgtaacgatg
     4081 actgctaccg ttatttttag tatccatact tgggaacaaa atcaaaaaca ggcagaaaac
     4141 atgacaagtg atgccgttca aagcacaagc agaatgctga atgataaaac aaccttgagt
     4201 aggatcatca aaaaccagct tgtagggaat tccgaaaaga tcgaaaatgt gacgacctac
     4261 ttaacaaaac ccatagatca gtatctgatg tatgtatacg aacagcaaaa cagtaccgat
     4321 gaattagttt ctttcccaaa tcaaatcaaa gatctctacg caaattatga agaactaagt
     4381 gcgatatata tcgtgttgaa tcaactaccg gagtattatg aatcttctag agaaaacaaa
     4441 ggtggggaaa taaaaaaggg gacaccaaaa ttactagacg ccttttatat caagttgcct
     4501 atcacacaag gaggagctga gtcaggaagt atttttattg gttttcaaaa gaaagaacta
     4561 gatatgattt tagaaaatct aacgtcattc aatggactct ctttatacat gatttctgga
     4621 acgacaaatc gactatacac attccatgat aataaaatag ctaaagaaac gtttgaaaga
     4681 caagaaatga tcatcacaga gagtctgaag aaaaattcac aattgccgat tgacgatctg
     4741 gaaaaaaaca attttgttca acaccaagaa ctgtctgatg attatcgtat attagctgta
     4801 ctagataaaa aaagtgtaaa aatcgaaacg ggaaaaaatc ttgccccgct gatcattggt
     4861 atcattattt tagacaatat actgcttatc tttctttata aaacattcaa acgatattca
     4921 cagcaagtat ctatcgtcat gcaggccatg gatcaaacag cagcagggaa tttagagacg
     4981 cggatcgaca taacaaacac ggaatatgaa ctgaaggaac tttcaatcgg tatcaacgaa
     5041 atgttggata gcatcaatca gtttgtagaa gacatctaca aattagaaat caaacagcaa
     5101 gacgcacata tgcgggcgct tcaagcacag atcaatccac actttttata taatacatta
     5161 gaatatatcc gaatgtatgc aatcagcgaa ggaagtgaag aattagcaga tgttgtgtat
     5221 gcgttttccg ctttgcttcg aaacaatacg aatcaagaga aaacgatcac tttaaaagaa
     5281 gaactggact tttgcgaaaa atatgtctat ttgtatcaaa tgcgttatcc taatcgagta
     5341 gcctatcatt ttatgatcga tcctgatttg gaaaagatcg aagtacctaa atttgtgatc
     5401 cagccattag ttgaaaatta ttttaaacac ggtattgatt ttactcgatt cgataatgcg
     5461 ctaagtgtca aagtcttgca ggaaggcaaa cgggtaagga tcatcatcaa agataatgga
     5521 aaagggatga ctgaaaaacg gctaaaacag attgaagaaa aactgtctca tcctaaagtg
     5581 gaacttcacg gttctatcgg tctgcaaaac gtgaatgaac gtttacgtgc cagcttcggc
     5641 agcagttatt atatgtcgct tgagaataat gagacaggtg gactgaccgt ttctattaca
     5701 ttcaaggagg gatgagcgtg tatcaagtgt tattggtaga tgatgaatac atgattttga
     5761 atgggttaaa aaagataatt gattggcaaa gtttaggctt tcaaatagta gcaacagcag
     5821 agaatgccaa agaaggacta gctgtacttg aacagcgaca gattgattta gtagtaacgg
     5881 atgtgacaat gccagaaata aatggactgg aatttattga agcggctcaa aaagaacgtc
     5941 ataattttga atttatgatt ttatcaggat accaagaatt cgattatttg aaaggaggta
     6001 tgcaattagg cgccgtgaat tatttgatga aacccgtgaa taagttcgaa ttggttgaaa
     6061 gcctcaagaa aatcaaaaca cggttagatc agcaaaatga acaaaaaaat caacaagaaa
     6121 tctatcaaga actgttattc agtcaatggt tgaatgaaga attggatgaa ataagtgaag
     6181 aagaaataat aggacaaata agtgagaagc agcataggat agtattgatt caactggcaa
     6241 gaactcatga tactcttatc aatagatggt tgcaagaacg gcaacaaatc ttttactatc
     6301 aacgaaatta cggagaccgt gtattgatca cgttgttgtt ggaggcagat gaagttgatg
     6361 gcttctgttg ttttgttcaa gaaaactttc ctgatcagga atggctgatc agtatcgggg
     6421 aagagacaga ttctttggac aaaatccctg aaacattcca acaagcgaaa gacaatctac
     6481 agttgcatca attttatgga gataaagacg agcatgtttt ttacgcagta caagcagaaa
     6541 caaaagatca aaccatcgat ttttcaagtt tcagacgcgt gctgcaaaat aaacaattgg
     6601 aagtcgctca aaaaatgatc actgattttt ttgaacagtt tcagttagca gccatgatgc
     6661 cggaagatat acgatattcc agttttttac tgtttatgga gattcaacgg gaattgattg
     6721 atctggaaga tgaggaatat ttacaaggaa tcgaaaaaat ccatcaggca aaaacggtgc
     6781 aagaacttca gcagctactt ctgtcattca tcaaaaagca tcagagacag aaaaagtaca
     6841 gtgacaatgt ggaaaaagta atcgtaatcc tgcaccagca ttatcaagaa ccacttacac
     6901 tcaaagaagt gtcagaaaac ctgcatttaa atgtcatgta tctaggacag ctgttcaaaa
     6961 aagaaacgaa aaaaagtttt tcagcttatt tgaaccattt acgtatggaa aaagcgaaac
     7021 aactgctttt gcacagtaat caaaacatca atgagatagc aagtgagatt ggctacaaca
     7081 acaccacgta tttctctaaa ttattcaaga aaatcgtcgg cagatcccct aaagagtacc
     7141 gagaaaacag tggagaatca taaaaaaaaa gccaatgtaa tgaaataact tataaaaaat
     7201 tgtctagaaa tctaattttg ggctgttctt attacttgag gcaaggcagc tttttcatga
     7261 cctctcgata aacgg
//
LOCUS       DANKHL010000147         7266 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000147 DANKHL010000000
VERSION     DANKHL010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.153"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            118..1539
                     /gene="arcD"
                     /locus_tag="PW101_002155"
     CDS             118..1539
                     /gene="arcD"
                     /locus_tag="PW101_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDL2514545.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            1553..2671
                     /locus_tag="PW101_002156"
     CDS             1553..2671
                     /locus_tag="PW101_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HDL2514546.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(2731..4023)
                     /locus_tag="PW101_002157"
     CDS             complement(2731..4023)
                     /locus_tag="PW101_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2514547.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            4155..4733
                     /locus_tag="PW101_002158"
     CDS             4155..4733
                     /locus_tag="PW101_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2514548.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            4862..5239
                     /locus_tag="PW101_002159"
     CDS             4862..5239
                     /locus_tag="PW101_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDL2514549.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            5172..7244
                     /locus_tag="PW101_002160"
     CDS             5172..7244
                     /locus_tag="PW101_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2514550.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         2426 a         1332 c         1566 g         1942 t
ORIGIN      
        1 gttcataata tccaataata tgaataaagt tgctttttgt taagcaagag cagttgcaaa
       61 atagaaggga atcttttgca agtgatttgt tcaattttat aagtgagggt gatggaaatg
      121 gaaaagaaat cgacgggtat tagcaaatgg ggactggttg ctctggttgt cagttcgtca
      181 atcggaagcg gtgtgtttgg gatcacaagt gatctggcta gttctgcagc tccagggcca
      241 gcgatccttt catggataat cgttgggatt ggtattttag ctttggtatt atcgctgaat
      301 catttaggag aaaagcgtcc agatctagat ggtggtattt tcggttacgc ggaagcttca
      361 tttggtaaat taggtggctt catcagtggt tggggttact ggttgtctgc gtggctggga
      421 aatgttgctt ttgcaacgat gctgatgagc gccatgggag aatttttccc gatattcaaa
      481 ggtggacaaa atgttccttc tattttattt gcaagtattt ttatttgggg tctgacttta
      541 ttagtaaaca atggagttga aggagcgagc gtcatcaata caatcgtgac gatctgtaaa
      601 ttagtgccat tattcctatt tttaatattt gtatttatcg catttaaagt gaatatattc
      661 actgctgatt tttggggtaa cgtatctgat aatctagtga atggaacagg gcagcaagga
      721 acgatctggt tgcagattaa aggttgtttg atggttatga tgtgggtctt tgtagggatc
      781 gaaggcgcaa gtgttttagc caaccgcgcg aagaaacgtt cagaagccca gcaagcatcg
      841 atcattggtc tgctttgttt attgtttatc tatatccttg cttcgatctt gccatacggc
      901 gtcttatctc aagaagagtt agcaactatt tcacaacctg ctatggcaaa tattttaaaa
      961 ggaatcgtgg gaaattgggg agcagcgctg atcaatatcg gcttgattat ttcgatcatt
     1021 ggttcttggt tgtcttggac gatgctccca gcagaaacaa cgatgttgat ggctcgagac
     1081 ggtctgttgc cgaaaggttg gggaaaaacg aataaaaaaa atgccccaac ttattcgtta
     1141 gttgttactg caatcttgac taatctattc ttattgactt tcttagttac tgattatgcc
     1201 tatcaatttg cctattcttt atgtacagcg gcaattttga tttgttatct acttgtcggg
     1261 atctatcaag tccagttttc tttaaaagaa ggaatcaaaa aacaagtagt gatcggtggg
     1321 attgctgtag tttttgaatt gatgggcatc attttagcag gattttctta tgtcctttta
     1381 tgtagtattg cctatcttcc tggtttttgc ttctactgga tcgcttgtcg tgaaaccaaa
     1441 catcaaatta cgggaaaaga aaaagtaatg attgggctga tttgtgtggc cgcactgctt
     1501 tcaatcgggt tgcttgctgt aggctggatc aagatataag ggggaaagaa acatgaaaat
     1561 cgcaaatttc ggtgtagaag aatggctaaa taaatgggaa aagaaagcgg tatatgatat
     1621 tgcacaaagc tcgatcgaag cattgacatt agacgaactc gttggcttag atgggactag
     1681 cgtaagtaaa tttttcgaaa aacgcaaaaa cgaaccattg gactatggct ggatcgaagg
     1741 atcagatgtt ttcaaacaag aagtcgcttc tctttatcaa accgtcgatc cggaaaacat
     1801 cttacaaacg aatggagcga ctggtgctaa tcttttagct ctatacgcgt tagtggaagc
     1861 aggagaccat gttgtttcga tgcttcctac ttatcagcaa ttatatgaca tccccaaatc
     1921 tttaggtgcc gctgttgact ttgttcattt gaaggaagaa gaagattggc agtttgattt
     1981 agaacagcta gaagccttag tcaaacctga aacaaaaatc atctgtttga atagtgcaaa
     2041 caatcctaca ggcacattgc tagatcggaa aactttagaa aaaatcgcag aaattgctcg
     2101 tacagtagat gcatatgtat tgatagatga agtatatgcg cctttaacgg ataaaggtga
     2161 attcttgtcg atcgtagatg tctatgaaaa aggtatagca accaattccc tatccaaaac
     2221 ctactccatt ccaggtattc gtattggctg gacagctact ggacctgagt tggcagaagt
     2281 ttttcgaaag tatcgagact acacgatgat ttgcggcgga gtcctgtcag atgatcttgc
     2341 tgtacatgca ttgaagaata aagaaaaaat attggcgcga aacaaaaaga tcattacaga
     2401 gaaccttgtg attttaaaac aatgggtagc caatgaaccg aaagttgaac ttgtcgctcc
     2461 caattacgtc tctacttctt tcattaaatt agccatcgaa gaagatgacc agacattttg
     2521 tatcaattta ttagaagaaa caggagtact attagtacca ggaagtgcct ttgatttacc
     2581 gaagcatgcc cgactaggtt actgttgtaa aaaagaaacc ttggaaaagg gattattcct
     2641 cttatctgag ttcttgaaaa aacaggccta aacaaaaaca ctgcaaggac agtctgtgac
     2701 aactgttcaa gcagtgtttt tcatttacgc ttattgtcta tgagaaggag agtgccctga
     2761 agctatggag actcctgaag ctttttcttc tgcagtttct tgctgatgga tctcttgtgt
     2821 attgtgtcgt acaagcaaga agttgaccaa aacaagaaga gaagtcgaaa gaaagacgcc
     2881 gcgataacca aatgcactgg aaacactgga gccgatcata ggccctacta cgttaccagt
     2941 tgcttggaaa gattggttat aactgaagat ccgtccagca gcttgatgag gcgaatattt
     3001 tgtgatcaat gcttggacag cagggagcag acaagcatca gagataccaa tcaaaaaacg
     3061 taaggcagct aattgccaaa cattcgtgac gaatgccatt gggatataaa ccagaatcgc
     3121 aaaacccaaa ccaatcgcaa gtattctttc actcccaatc ttgtcaccta agcgaccaaa
     3181 tcttggagcg gctatcaaag tggcaatccc aggtatcgaa gcaatgattc cgctgatcaa
     3241 cgtaacattc ccatgtccat gcagcaattg gcgaatgtac agactgatga ttggactaat
     3301 ggaattgttg gatgcttgga tgatcatcgt tgtgacgaac ataccgatga cgacgtgtgg
     3361 atatttcaag tctttgaaaa tctgtttggc agaaaccatg ttttcttttt tcactgggac
     3421 aaatttttca tggacgaaaa acagactcaa caagaagacg aaaaataaga tgatccctgt
     3481 aatgaaaaag gtagggcgat agccaaacgc tgaagcagag atcccgccaa gaagcggtcc
     3541 aagtaatgtt cctgtaacag aaccagttgc taaggttcca agaacttgac cacttttttc
     3601 ttttggcgtc ccagttgcga caagggcagt ggcattactg atataaccag aaaaaatccc
     3661 ttgaagcaga cgcaatgcga ctaattgata cacactagtc acgcaaccca tcaaagaaat
     3721 gacgattgcc attcctaatg aagcacgcaa cagcatcaat tttcttcctt tttggtcggc
     3781 taattttccc caccaaggag agataagcgt agtcaccaag aaagtagatg aaaaagtcaa
     3841 tccgctccaa aagttcaatt gtgacgtact ataattacct aacgtatcta tgtaaagtga
     3901 cataaaaggc atgactaaac taaatccgat accagccata aatgttccaa accataagac
     3961 aaacaagttt tgttcccaag tttctctttt cctaaataat ttttccttta ttcttgcagc
     4021 caattagact ccttctttct gaaaattttc atctacagct ctttattata aactacaata
     4081 tgtagtttat caatgaaagt ctcttttttc tctttctttt tcgtggtaaa ctgaaagagt
     4141 gaaggattta ttttatgaga ggaaacatgg aaatgaaaaa aagaaatctg tctcaagaga
     4201 aaatcatcga ctgctttcga gaactagcag aagagatgga tgttcagcaa ataacctttc
     4261 agcatttagc caaacgcttg gatatcaagg ctccctcgct gtataaccat ttcaaaaata
     4321 ttcgtgatgt caaaacagcg ctaacagcga aacttctgca ggaacttaat gaccagctgc
     4381 gacgggctct tgtgggaaaa agtggtcagg aagcactgca gatctatgca gaaacttatc
     4441 gatcctttgc tttttcaaat caagcagtgt atgagttact gatcagtgta ccgcatacca
     4501 atgaagctgt tttattagat ggaatccatg aaacgaatca gatcattttg cagatatttg
     4561 aggcttttca attaacgaaa gaagaaaaag ttcatcgaag cagggaactt cgtagtatga
     4621 tccacggtta tctttcctta cgttttttag gatactttac aaaagaacca acaatcgatc
     4681 cagaagacag ctatttttgg atgatcaata actttatcgc tactttgcct taaacaatca
     4741 gattcatttg taacaatttt aagatgttca ttaaatgacc cttttataag agaaaattct
     4801 tgcaactttc ttgaaaagtg actaagatga gtctagaaaa gctgaaagaa ggagcacgag
     4861 tatgtatcaa gtaattacca tgttcgggga caatgagcct tggtggtttt ttgaagactg
     4921 ggaagaagat attgaagaag aagaaacatt cgagtctttc gaagaagcac gacaagcgta
     4981 cgaaaaacaa tggaacgaaa tccatcaaaa ttatgaatat atcaatgcaa aaagtaactt
     5041 tctcagcgcc ttttggaatg acggagatga gcgctggtgt gaagagtgtg acgatgattt
     5101 acagcaatac aagggacttg cgttgttgaa aaatcatcag cccatcacag tagaaagcag
     5161 aaaggaattt tatgaaacaa ctaattctag cggaaaaacc aagcgttgcg aaagacctaa
     5221 gcaaggtgct tggtgctaat caaaagcaca aaaattatta tgaaggaccc aaggtgatcg
     5281 ttacttgggc ccttggtcat ttattaggat tgaaaatgcc ggaagatctg aataaggaat
     5341 ggcagagctg gcaaatggac acgttgccaa tgatccctaa aaaactagga atcaaaccac
     5401 ttcctaaaac aggacatcaa ttaaaagcaa tcaagcagct ggctcagcga aaagatgtct
     5461 cagaagcagt cattgcgaca gatgccggaa gagaagggga acttgttgct cgctggatct
     5521 tagagtatgt gcatttcaat aaaccagtga agcggttatg gatctcttct caaacagata
     5581 aagcaatcaa aactggattc aagcaactaa gacctgcgaa agaatatgat gcattgtatg
     5641 aatctgctct tgcccgggca aaagcggact ggctggtagg attgaatgtg accagagcct
     5701 taactgtgaa gtacgcggat aatttatcag caggccgtgt gcagacaccg acactagcat
     5761 tagtgagaga ccaagaaaag aaaatcgagc agttccgtcc acaaacgtat ttcacgatca
     5821 cgttgcaagc tggaaaagaa caagccaaaa tgcttcaaaa aaatcaattt gctttaaaaa
     5881 cacaagaaga agcacaaaaa cttgttcaat tgatcagtac aagcaaagga actgtcacag
     5941 aagtccaaga aaaattaaaa acggaatctg cgccattgcc ttatgattta acagagatcc
     6001 aaagagaagc caatcaacgc tatggctatt ctgcaaagaa aacattaagt cttgtacaaa
     6061 gtttgtatga gacccataag atcgttactt atccgcgtac agacagcaaa tatctgacaa
     6121 atgacatgaa aagtacgatg aaagaacgat tgcaagcagt cagcgacttt gctcctgagg
     6181 tcaagcagta tttgaaaaac ggagccatcg tccaacagac aaaagtattc caagacagta
     6241 aagtgaccga tcaccatgca cttttgccaa ctgaaaacag agcacgttat gaaaaattaa
     6301 gtaatgaaga gcgaaaaatc tatcaaatga ttgtctctcg ttttttagga ctgtttgctc
     6361 aaccccataa agtcagccaa acaaaagtta cggttgaatt tggtaaagaa cgattcattt
     6421 ttcggcaaaa tcgtgtcgtc caagcaggat ggaaaggcga aactgaaact gagacggaaa
     6481 cagtcaaatg ggaaaaagga atgagaatca atcccgattt cgccatcaaa aaagaactat
     6541 ctgcaccgcc taaaccattg tcagaagcaa gcttgcttgg tcaaatggaa aaatacagtc
     6601 tgggaacccc agctacgcgt gcagagatta tcgagaaact aatcaaatct gaactgatgg
     6661 aacgtacccc tcaaggactg caagtatctc ctaaaggtaa gcaattatta gtattggtaa
     6721 atccctcttt agtaacacca gatttgacag aaagctggga aaaagacctt gaagctattg
     6781 ctgcaggaaa gaaacaagca ggaaccttct tgaatggaat cgaaaaagaa acaaaacgct
     6841 tggtgaatga gatcaaatca agcaaacaag aatatcaaga tttctcgatt acccaaaaga
     6901 aatgcccaga atgtggggcc aatctgcgcg agaaaaacac acgtgacggc aaaatctatg
     6961 tctgcaccaa ttcagattgc aactaccgca gaagaaaaga tccaaaagtc tctaatcatc
     7021 gatgcccgca atgtcaccgg aaaatggaaa tcatcgaagg taaaaacggt gcttatttcc
     7081 gttgtaaata cgatggaacg acagaaaaat tgcttgataa aaaagagcag aagaagaaaa
     7141 tgaccaaaca tgaagaacga cgcttgctga aaaaatatgc acaagcagaa gaaccagaag
     7201 aaagcccatt agcagcagca ctaaaagcag caatgaagaa gtaataagat atgaaagagg
     7261 ttgtga
//
LOCUS       DANKHL010000148         7138 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000148 DANKHL010000000
VERSION     DANKHL010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.154"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..327
                     /locus_tag="PW101_002161"
     CDS             <1..327
                     /locus_tag="PW101_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL2514551.1"
                     /translation="GDNIVNCLKLVGNNDYSQFYDWYKKFKYAIKSAEKEQKIQLKII
                     YEICNNKYFVDYVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNKALELIELQIS
                     EGIDKQ"
     gene            608..3301
                     /locus_tag="PW101_002162"
     CDS             608..3301
                     /locus_tag="PW101_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HDL2514552.1"
                     /translation="MKIVKEKIASRKIHWLALSILVLMFIVVFTGNVVQASIFNWFDD
                     TESQTEFLNNPVYQSYLKKYNGNFYSQFGLWIGWSVIKSMFAIVDGIQNMIPEVLDLF
                     SFIESTGMNKVYQSVLSTVVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSVALI
                     LLMPTLIGSTEKLSGIMFAKTLYNDATSINNQDGGVAWSLIEQGVTDLAYINKTDQYS
                     TIDKTNDRNNLTKENFWKTDLTQVLTDNVIDKMEKENPTADNLRYELVENSDNEFVAT
                     KFSDNFLSTFVDGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRV
                     LGVLVFATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFINYLNTGEGISANIF
                     IKTIAYICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRS
                     KGGKNNGGNDQAEGEERKPERNFGQSLSKKANKTGKALGYAHERGLSGLASDGSTMAS
                     ERATKPFKSMRDIANDTKSKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPT
                     RLSDRKDGAKVENADSIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMH
                     GQAKSAEELINQKRHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAK
                     NSNTGLEKLVKENPQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQG
                     TKTIDVRENLQSSSTSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDV
                     RENVQSSTSSQGFKTVDVRENVQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIID
                     SEIRENDKGSKPRRRFTYEDNELFRDTLNDPNPLFDSLLKK"
     gene            3315..3512
                     /locus_tag="PW101_002163"
     CDS             3315..3512
                     /locus_tag="PW101_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514553.1"
                     /translation="MQLILQISTHDFLELDELCESVKKATNEKITVTNYIELMIQEEL
                     KMKNEIESDFKCCSLEKNVRG"
     gene            3516..4127
                     /gene="mobC"
                     /locus_tag="PW101_002164"
     CDS             3516..4127
                     /gene="mobC"
                     /locus_tag="PW101_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HDL2514554.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            4112..5503
                     /locus_tag="PW101_002165"
     CDS             4112..5503
                     /locus_tag="PW101_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL2514555.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            5719..6033
                     /locus_tag="PW101_002166"
     CDS             5719..6033
                     /locus_tag="PW101_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514556.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGVFGSKANMFRVMVSNLP
                     VLASPSNNKFNDSESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            6026..6520
                     /locus_tag="PW101_002167"
     CDS             6026..6520
                     /locus_tag="PW101_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514557.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTIDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLKNDTEKPIRPQQW
                     SIENKDGEELYEYAMILDNPEYIESDKEETVNYYYELPIERQDYQIVVRRANGYFVGL
                     PFTL"
     gene            6561..6911
                     /locus_tag="PW101_002168"
     CDS             6561..6911
                     /locus_tag="PW101_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514558.1"
                     /translation="MIFENESEEIGRMAEENKVVPFETLVINKEEFEQLFLDFFIQYD
                     RIYDFSSSLDLFELEAEIIENSREKNGKIIISVEKFNELFSETFEIDPDYYQPQEVFR
                     EFRNQIIRSSNIEN"
BASE COUNT         2708 a          955 c         1393 g         2082 t
ORIGIN      
        1 ggggataata ttgtaaactg tctgaaatta gtgggtaata acgattacag tcaattttat
       61 gactggtata aaaaattcaa atatgcgatt aaatctgctg aaaaagaaca aaaaattcaa
      121 ctgaaaatta tctatgaaat ctgtaataac aagtattttg tagattatgt tagagaacag
      181 ctatcaatga ctttacgtga cttgatacga agagcaaaaa cagatcaccg tatcaaacaa
      241 aaagacgatt atatctttgc ttctctaaaa aataaagcgt tagaactaat tgaattgcaa
      301 atcagtgaag gaatagataa gcaataagaa tagaattgag tcaaatgaag tggaggggta
      361 tctccgcttc atttgagaac tttgaatata ggaggggtta ttttgattga aaatcgttta
      421 atagatcaag tttggtgtct agatcaaaat acagataatc tcgaattaat cgagactatt
      481 tgcttcaaca ctatagttct cgatttaaga tctgaaaatg acaattgtgt aactcatatc
      541 ataattgaca gaaaaacagc tcagaagctc tctgagagtc tcagaaaatg ggctgaggga
      601 ggtaataatg aaaatagtta aagaaaagat tgccagtaga aaaatacact ggttagcttt
      661 gagcatactt gttttaatgt ttatcgttgt tttcaccggt aatgttgtcc aagcaagcat
      721 tttcaattgg tttgatgaca cggaaagtca aactgaattt ttgaataatc ctgtctatca
      781 atcgtattta aaaaaatata acggaaattt ttacagtcag tttggattgt ggatagggtg
      841 gtctgttata aaatcgatgt ttgcaattgt agatggtatt caaaatatga ttcccgaagt
      901 gttagatttg tttagcttta ttgaaagtac gggaatgaat aaagtttatc aatcggtctt
      961 aagtacagtt gttattggtt taatgatact ttcactgatg tttgttggtt acaaaatgat
     1021 tactggaaaa ggaacagtgg atttaaaatc ggttggaatg aacgtagtga tgtctgtagc
     1081 attaattctt ttaatgccga ctttgattgg atcaacagaa aaactttcag gaataatgtt
     1141 tgcaaagact ctttataatg acgcaactag tatcaataat caagatggcg gtgtagcatg
     1201 gtcattgatt gagcagggag tgacagattt agcttatatt aataagacag atcaatattc
     1261 gacaattgat aaaacaaatg accgaaataa tctaacaaaa gaaaatttct ggaaaacgga
     1321 tcttacgcaa gtattgacag ataatgtaat tgataaaatg gaaaaagaga acccaacagc
     1381 tgataatctg cgttatgaat tagttgaaaa ttctgataat gaatttgttg ctactaaatt
     1441 ttcagataat tttttatcaa cctttgttga tggtatcaaa tcaggatatt atcgctatca
     1501 agcgaacctg tggggaattt cttttggatt gattgcttta gcggtagcgt atgtttttag
     1561 tgctttcatt ataattacag caatactcga gttagcattt aaacgtgtac tcggagtttt
     1621 agtatttgct actgacttag aaacaggaca acgatcaaaa caagttttgt cagatatttt
     1681 acagtgctat ttgacagtcg gatttcaagg gttcggttta tctatgtttg caatgttcat
     1741 aaattaccta aatactggcg aaggtatctc agcaaatatt tttataaaaa caattgctta
     1801 tatctgtgct gtgtttgttt taatcaaagg cagtggaaca gtcatgagat actttggtgt
     1861 ggatattgga ctcaaagaag gctatgggca acttgcttct gcttttggaa tgggtgcaat
     1921 gctattccgt aaaggaacag gtggtatgaa ccggtctaaa ggtgggaaaa ataatggtgg
     1981 aaacgatcaa gctgaagggg aagaacgaaa acctgaacgc aactttggac aatcactttc
     2041 taaaaaagct aataaaacgg ggaaagcgct tgggtatgct catgaacgtg gattatcagg
     2101 tttagcttca gatggatcta caatggctag tgaaagagca acaaaaccat ttaagagtat
     2161 gcgtgatata gctaatgata ccaagagtaa gtttaaagaa ggtttagatg atggtactgt
     2221 gtccgctatt aataaaaata gcaaacctat gttagctaaa aataaagaag atgaaactgg
     2281 aaaatatgct gattccatgc caacacgtct ttcggataga aaagatggtg ctaaggttga
     2341 aaatgccgat agtattatgt ctagttctga acgtatgcgt gaagcaatga aaaataatgc
     2401 tgaatcgaat aataatcctg taagtgcaat tcagcaaaaa gtccaacaag atattgaaga
     2461 acgtaaaaat gctatgcacg gtcaagctaa gtctgctgaa gaattaatta atcagaagcg
     2521 tcatgaagcg aaatacacgc ctgaagcaat gaatcgcgag gaaataatgt taaggaaacg
     2581 tgtagaaggg cggacaggcg ctaatattga cggaaaagaa gcgttgacca aagaaagaat
     2641 tcaagaagct aaaaattcaa atacaggatt agaaaaatta gtcaaagaaa atccacaaaa
     2701 ttctacaagt agtcatggtg gccgatcagt agatgtccga gaaaatattc aaggttcact
     2761 gaatggtcat gttggacgga atgttgatgt aagagagaat ttacaaagtt cttcaaatgc
     2821 tcaaggtact aagacaattg atgttcgaga aaatctgcaa agtagcagta caagtcaacc
     2881 aaaaacagta gatgttcgag aaaatgtaca aaattctaca agtagtcaag ggtttaaatc
     2941 agtggatgtc cgagagaatg tgcaaggtag cagtgcaagt caaccaaaaa cagtagatgt
     3001 tcgagaaaat gtacaaagtt ctacaagtag tcaaggattc aaaacagtag atgttcggga
     3061 gaacgttcaa aaagatagtg gagaaatgaa agaaaaaaca caaaaaatca atatcgttga
     3121 agacagaaag tcagcgaaaa aatttgattc aaatcatgaa acagtgatta ttgatagtga
     3181 aatccgtgaa aatgataagg gatcaaaacc aagacgaagg tttacctatg aagacaacga
     3241 gttattcaga gatacactta atgatcctaa tccacttttc gatagtttac ttaaaaaata
     3301 aagaggtgat tgtcatgcaa ttaattctac agattagtac acatgatttc ttagaacttg
     3361 acgaattatg tgaatcagta aagaaagcaa caaatgaaaa aatcacggtc actaattata
     3421 ttgaactgat gattcaagaa gaattaaaga tgaaaaatga aatagagagc gatttcaaat
     3481 gttgctctct agaaaaaaat gtgaggggat aatcaatgga tgataaacaa aaaatgtacg
     3541 aaaaaaatcg caaacatagt gaggccttaa attttcgtgt cgataaaaag acttatcttg
     3601 aactgacatt gattgcaaaa caaaggggaa tttctagagc agacgtttgc cgtgagcggt
     3661 taaagggact caaatatgaa ccgccgttgt tagctgaaga tgatagggta gttttaacca
     3721 aacagttaaa tcaaattggt aacaatttga atcaagcggt aaaggcatta aatatcattg
     3781 ctaaatattt tagagaagaa tctgaagata aaaaagaaaa gagcctacat tttagttttg
     3841 atgctagccg ttacaacatt tttaaagatg aacttgcgaa tagcatagca aaaaagttga
     3901 tgattagtta tccagatgac atttttttgg agtccaatcg gagtgccccc tcttttttta
     3961 aattatcaga agtagaaaaa gaatatttcg aatattggat agaaaagcaa actagaccgg
     4021 gtacgaaaga agagtattcc caaaaagttg atcggaattt aattcactca attcagatta
     4081 ttgcaagttt aaaagatgag gtgaaacaac tttgggagct actataacat cgaaaggacg
     4141 caagaaaaat ttatcctcgc tattacaata tgcagctaat ccttcaaaaa aagaaaaatg
     4201 tgttgcagtc tcaggaataa attgtagtga tgatagtgag gaagcactgc aagaaatgaa
     4261 tttagtacgt accaagatga ataaatctaa agatatagca caagcgtttc atgttattca
     4321 ttcgtttgat aagagtactt ctaaagagtt aagtattgat aaaatgcacg aaatagctgt
     4381 cgaatttgca gagaaagctt ttccaaattc gcaaatcatt gttgcaagtc acaatgataa
     4441 agatcatttt catagtcatc ttgtcattaa taatattaac atggaaacat gtaaaaggtt
     4501 gcgtattgac cctaaagatt tagaactaat gcgaaaaatt aacgatcaag tcctcaatga
     4561 acatggtctt gaatcagtga ataatgagta ctatgaaaac ttagaaaaga gaaccaatct
     4621 ttattcaaac gatccttcta agatgaaata tggaaaaatt aattatcaag agtttgtgca
     4681 aaatgcgatc gaagcaacgt tggaaaaatg cgaagtgaat tcttttgaag atttttcgga
     4741 tcatttggca aaagaatata acattgaagt ttatcaattt tcaaaaacaa ataacaaact
     4801 tggctatgtt ctttacaaac aaggtgtaaa tttctatgag cgtagaggtg agttaattac
     4861 tttaggaaga gataaacaat ctagaaaaga aaaaagtcag tatgtcgaac gtacttttag
     4921 tgcaagaaag ttagggaaaa gatattcatt tgaatctgtt tctgaacaac ttgaaaagaa
     4981 tttagaagaa tcacatataa aaaatttttt taatatggaa agagagcaag ttatgtcgct
     5041 aattagtgga gtaaattaca aaactggtga gttaaatgta ccttttgtgg aattagattc
     5101 catcgcacaa aagcaattta gcgaccgtga aggcgatgaa aaattcatta aatatgatga
     5161 cttcttgcga gaggtagaca agcattttgt aaacacaatg tctttagaag atgatcaaga
     5221 atacacgatt aaacttcata cggatgtgaa tcctcaaact ttacgtagtg cgaaaagtaa
     5281 aacaatttct attaaaaaga ctgatcagaa agagttattt ctaccggcaa agggttttga
     5341 aaattgttct catacacctt ctgctaaaca gattcactat caaatcagtc gcataatgaa
     5401 tgacgatgta aaaatgtatc gtgctaaaaa ggattatgaa ttcacacaac aaatgattga
     5461 aaatcagcaa aaaaggcaac aaaattttga aatggaattc tgaatgggct tgttagtgga
     5521 ctttccagtt ttttgcctat gaagagttgc ttggaaagaa gcagttttta ggacatacgc
     5581 cagctaccca tctacttgcc agtcaatccg ccccgctcac gctaggctac tttctggcaa
     5641 gtcagatggt gggtaaatct ctgagggagc aagctcccta cagactccct cccaaaaaaa
     5701 gaaaggtgga agaaaaaaat gaactatcca tattttaaag tctcggcttc agaagaaact
     5761 aaagaaatat tcaacaattt ttacaaccaa aacaaaggag tttttggttc caaggcaaat
     5821 atgtttcgtg ttatggtttc aaatttacca gtgttagcta gtccatcaaa taataaattt
     5881 aatgattctg aaagcattaa atttgaacaa aaaatttcag aactagaatc aatgataagt
     5941 aatgaagtaa ttgaaaagct tgatgacatt gatcaaaagt tatcgtattc tctaaaaaac
     6001 aaatacaaaa cggaggagaa aaaagatgtt taaagtttac ttcccgtttc agtggctcgt
     6061 taaaggaatt cttttagtgc tactaggatt gattcttttt ggaagttatc taacaattaa
     6121 agccagacat tttccagaag aaaagccaat aaaaattgta acaatagatt taaaaatagg
     6181 tgaaacttat caggcagatg gggttgaact aacatttaaa aatattgaag tgaccgaaaa
     6241 aagaaatgaa tttgcacaac atgatcgtgt attacaagtg acctatacac ttaaaaatga
     6301 tacagaaaaa ccaatacgtc cacaacaatg gtcgattgaa aataaggatg gtgaagagct
     6361 ttatgagtat gctatgatat tagataatcc agagtatatt gaaagtgaca aagaagaaac
     6421 agtaaattac tattatgaat taccgattga aaggcaggac taccaaatcg tagtacgtag
     6481 agcgaatggt tattttgttg gattaccctt tactttgtaa gtcttatttt gttagtggct
     6541 tttaagttaa tatcattttt gtgatttttg aaaatgaaag tgaggaaatt ggaagaatgg
     6601 ctgaagaaaa taaagttgtt ccgtttgaaa cattagtgat aaataaggaa gagtttgagc
     6661 aactttttct tgattttttt attcaatatg acaggattta tgattttagt tcgtcactag
     6721 atttatttga attagaagca gaaataatag aaaacagtcg agagaagaat ggtaaaatca
     6781 ttatttcagt agaaaaattc aatgaattat tctcagaaac atttgaaata gatccagact
     6841 attatcaacc acaagaagtt tttcgagagt ttagaaatca gattattcgt tcaagtaata
     6901 ttgaaaattg agtcaataaa agaagcgttg aagaaaagtt ccttctttcg acgcttcttt
     6961 ttaaaagatt ttacaaatgt cttatggaga tttgcttcaa tgactacttg tagtcatttc
     7021 gtttaaaata attatgttac acccttatgt gtcacagaga aatactgata taataagaaa
     7081 taaggtatgt aacaatagag tatacttcta tgttacatat tgattatttt gaaaaaat
//
LOCUS       DANKHL010000149         6983 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000149 DANKHL010000000
VERSION     DANKHL010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.155"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(72..1175)
                     /locus_tag="PW101_002169"
     CDS             complement(72..1175)
                     /locus_tag="PW101_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL2514559.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(1397..1867)
                     /locus_tag="PW101_002170"
     CDS             complement(1397..1867)
                     /locus_tag="PW101_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HDL2514560.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            2200..3840
                     /locus_tag="PW101_002171"
     CDS             2200..3840
                     /locus_tag="PW101_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDL2514561.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            3994..5316
                     /locus_tag="PW101_002172"
     CDS             3994..5316
                     /locus_tag="PW101_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HDL2514562.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(5378..5809)
                     /locus_tag="PW101_002173"
     CDS             complement(5378..5809)
                     /locus_tag="PW101_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2514563.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(5873..6709)
                     /locus_tag="PW101_002174"
     CDS             complement(5873..6709)
                     /locus_tag="PW101_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL2514564.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         2078 a         1445 c         1261 g         2199 t
ORIGIN      
        1 acatccttga actataaaaa taggagtacg gcatcatctg ccgcactcct atttttttcg
       61 tcatttgttt cttaaataaa acgattctca cgcaatttta tttttttgat atcttctact
      121 gtttgtgttc cagctaattg catgaccatt tccaattctg ttttaaagaa atcaaacact
      181 tgatggactc ctgtacttcc tcctaaagac aatccataaa taacgggtct gccaatagca
      241 acaagatctg cacctgatgc gattgctttg aaaacatgct gtccgcgtcg tacaccgctg
      301 tcaaacacga ttggtacacg tttatctact gcttctgcca catattgcaa cgaatcaaac
      361 gctgctggac caccatctag ttgacggcca ccatgattag atacccagat tccgccagca
      421 ccagattcta atgaacgata gacatcttct tctgactgga cacctttcac atagactggc
      481 aaatcagaat gagcagcaat aaattctaca tcttttggac taagcttctg tttggaagat
      541 ttgtacacag catccatcgt ttggcctact cctgattgat aagcctgaac gatcggcatt
      601 ggcagtggga aggtaaaccc atttctgcgg tctgtttcac ggtttccgcc aactgttgca
      661 tccgctgtca atacgattgc ttttgctcca ttacgcttgg ccacttcaag aatatccaaa
      721 ttgatcccat catctttgct catataaaat tgaaaccatt gcggtgcttt ctccccgcct
      781 gcttctctta tctcttctaa tgtacaagaa gcgtaagaac ttgctgtata gatcgtccca
      841 aaatctgcta caccttttgc agaagctttc tccgccttca catgagctaa gccatgagca
      901 gcaactggtg ccatgatgat tggtgcagtc agcgtttctt tgtcaaaatg tgtagttgta
      961 tcgggtaatt ctacatcttt taatacatga ggaataatca gctgatgatt gaatgcccgt
     1021 tcattttctt gataagtaaa taagtcgcca gcaccactgc tgatgtaacc ataaccacca
     1081 gctggaatca cttgtgaagc ggctatttct aaatcttcca tattgataaa atccactatt
     1141 ccttcatttg tacctgcttg atacgttttt tccatttcaa tgccttccct ctataaaaaa
     1201 ttgaatgaat aatatgatag cgtttacaca ctttatatta ctcttccgta ttgatacagg
     1261 caaataatct gtttacattt cagtcattcc agttgttact actctgaaag aaatgtttag
     1321 aacacaaata gataaaaaac gatgggactt tgacaaaagt cgtgtcaaag tcccttttac
     1381 gaaatactgt ggatgcctat tgaaataagt gcgggttgtt tctttgtatt tcaatcgttt
     1441 cttgccattc ttcgtttaat tttggattac cgttttcttc cttgaacgca taaggtccca
     1501 atccaaaaag ctcagttagt atttgattca cgattccata tagctcggtg tgatcggaca
     1561 catgattatt gaagatcatc tcattatcac cacgtttgaa tctcacattc gggctctcat
     1621 gatgaacctc tatgcagttt tccccttttt ttaactgacg gatttctgaa aacatctcat
     1681 aaatatcttc tccaataaaa ccttcagcaa gcgccagact gtccacaaga ccgcctactg
     1741 tcaactcaga gattatcgct ttgttggcac caagctttaa tgtatagctt gttgcttcta
     1801 ctacgccgca tgcattgcaa ccaccttctg atagttcgct ttttctgatc aattgaactt
     1861 ctttcatttt ctgttcctcc tcttagtttt ctacttatgt attttttatc acctgacgta
     1921 agtgtatttt atcgtcacga tcacgtgatt tattcttcag agattctatt tatctatcta
     1981 acatatcctt tattcttcta cttttctttc tttagcatat aagagatacg catagtaatc
     2041 aagcaaggat tgttgatata aaaagaacaa ataccgctat tttgatttct tttgactcta
     2101 ttactcatat aaataaaatt tattatacct gctctttttg actatatcct catctgactc
     2161 ctatacttcg tttgtattaa aacttaggag gaatcattca tgcttacagg gctaaactta
     2221 ttaaataatc cttttctaaa taaaggaaca gcttttacaa aagaagaacg ggcaaaatac
     2281 ggaatcactg gtatgcttcc aagtactgtc caaacattgg aacaacaatc tgtccaagct
     2341 tacggtcaat acttaagcaa acagaccgat ttggaaaaac gtatcttctt aatgaatcta
     2401 ttcaatacga accggacatt gttttataaa ttgatgggcg aacatttagt tgaattcatg
     2461 cctgtcgttt atgatcctgt cgttgcggat tcaatcgaac aatacaacga aattttcttg
     2521 aaaccacaag atgctgcatt tctttcaatc gacgaaccag aaaatatcaa agcaagtttg
     2581 aaaaacgcag cagatggacg cgacattcgt ttgatcgttg tgacagatgc agaaggaatc
     2641 ttaggtatgg gtgactgggg tgtgaacggt gttgacatcg ctatcggtaa attgatggtc
     2701 tacactgctg cggctggtat caatcctgca caagtcttgc ctgtatcaat cgacgctggt
     2761 actaataacg aagaattatt gaataatcca ttatacttag ggaatcgtca tgctcgtgta
     2821 gaaggcgaaa cttactacga attcattgat caattcgttc aatcagctac tgaattattc
     2881 ccagaattgt tattacattg ggaagatttt ggacgaggaa atgctgcaac gatcttagaa
     2941 aaatacgaag ataaaatcac tactttcaat gatgatatcc aaggcacagg aatcgttgta
     3001 ctagctggtg tattaggtgg attgaatata tcaggtgagc aattgaaaga tcaaacgatc
     3061 ttgacatttg gtgctggtac tgctggtgta ggtatcgcaa acatcctttt agatgaaatg
     3121 atccgtcaag gtgtacctga agaagaagca cgccaacatt tctatcaagt agataaacag
     3181 ggtttattgt ttgaagatac aaaaggattg actcctggtc aaatcccatt cgctcgtaaa
     3241 cgctcagaat tcactaatgc tgatgaattg acaaacttag aagcagttgt aaaagcaatc
     3301 catccaacga tcatgatcgg tacttctacc caacctggag cattcactga agaaatcgtg
     3361 aaagaaatgg cggctcatac cccacgtcca atcatcatgc ctttgtctaa tccaacaaaa
     3421 ttggcagaag caaaagcaaa agatttgatt gaatggacgg acggaaaagc tttagttgga
     3481 acaggtatcc ctgctgctga tgtggaatat aacggtgtaa cttaccaaat cggtcaagca
     3541 aataatgcgt tgatgtaccc aggtttaggg cttggtttga ttgcttcaac agctacacga
     3601 gtaaatgctg aaatcatttc tcaagctagt cgagcattag gcggaatcgt tgatgtaaca
     3661 aaaccaggag cagctatctt gcctccagta gcaaaaatca ctgaattctc tcaaacgatt
     3721 gctgaaactg tcgcaaaatc agttgttgca caaaaattga atcgtgaaga aatcacggat
     3781 atcaaagaag ctgttgaatc tgctaaatgg gttcctgaat ataaatcttt agaagattaa
     3841 gattgttata gtaaaagaac atacagaaac tttgtatgac gtctaaaaag aaaaaagcga
     3901 ctgcggcaaa acagcagcca gccaactact gctcttttgc cgcttctctt tggctttttt
     3961 catacgatac agaataagaa aggatctgta aaaatggcac aaaaaacaac aaccgcaact
     4021 gcagttgagg aaaagaaaaa agaagggttt tggtcaacaa aaattaccgg cgtaagtttg
     4081 cccctttatt tgatcatggt ccttgtcctg atcattgcaa tggctttagg aaaattaccg
     4141 acgaatatga ttggcccaat cgctgttttg gtcattttcg gtaatttatt tcattttatc
     4201 ggaacaaaaa tccctatcgt caaaagttat ttaggtggtg gctccgtttt tgcgatcttt
     4261 gcctctgccg cgattgctac atttgggatc ttacctgagt atgtagtaaa atccacagaa
     4321 aactttgtca gcaatatggg ctttttagac ttttatattg ctgccttgat tactggtagc
     4381 atccttggta tgaaccgtaa tctattgatg aaagcttctg ttcgttttat tcccgttgca
     4441 cttatttcaa tggtcgtctc cttttttgcc gtaggactcg taggtatgct tatcggaaat
     4501 ggttttgcaa attccgtttt atatatctca ttgcccgcaa tggctggtgg tgttggagcc
     4561 ggagctgtac ctttatcaac gatttatgca aatgttttag gtacggacgc ttcttctatc
     4621 atttcccgcc tgattcctgc gacagcaatg acaaatgtcc tagctatcat tggtgcagcg
     4681 ttagttgctc gtgcgggaca atcaatgcca aaattcaacg gtaatggtaa attaatggaa
     4741 aaaggcgacc ttggcgatgg aaaacctaga aatgtaaaac cttccattcc acaattaggt
     4801 gtcggtttga tggtatcatt gactttcttc atcttaggac aaatctgtaa ctattttgtt
     4861 cctaaagtcc atgcttacgc attcatgatc attatcgttg ttatctgtaa aatcgcaaac
     4921 ctattaccag aatattacga agatgcagct atcatgttca acaacttgat tgtcaaaaac
     4981 ttgacagcag ctgttcttgc cggtatcggt attgcgttat tgaatctaaa tgtattggct
     5041 tctgctttga catggcaatt tgttgtctta tgtttgacaa gtgttatcgc gatttcagta
     5101 gtttcaggat ttgtcggtcg tttgtttggt ttatatccaa tcgaatcaac tattactgct
     5161 ggcttatgca acaactcgat gggtggtaca ggaaatgtag ctgtcttatc tgcagctaat
     5221 cggatggaac ttattgcttt tgctcaaatg ggcaatcggc ttggtggtgc catcgttttg
     5281 atcatctcag gtttcttgat gcaattactt tcttagtcaa aaaaaatacc tgatcatcaa
     5341 gtatcggctt gatgaccagg tattttttga taaaaattta caggattttc aaaaatcttt
     5401 tttgcatttt atcggaatga taaccgatgc gttcataaaa tcggtgtgct tctactctgt
     5461 attctgcaga attaagtctt attgctgata ttcctcgtct tttcgcttct tctacgccag
     5521 tcatcaaggc ctttccgatt cctctttttt cagcatgttt agacacagca agtgccataa
     5581 tattgaacat cggatcgctg aaaatcgatt cataggactc cgcttgtaca tagccagctg
     5641 cttttttaga agtaggatct tcatagacca agaaaaaatt tttttctggc tccttcaaca
     5701 gtttcttcat ttgtctcacc gtaacggtta atggtacatc atatcctaat tgttccgcat
     5761 tgatttctcg tagtgcttct gcatctccta aacgcatttc ccgcaacatc caaatcacca
     5821 gccaattttt atttttttaa atgaattttt ttatcacagt atactccttt tttcatttag
     5881 aacaatatgt ttcagtgatt ttgacttgcg ttagtctaga ttctcctaat gtttgtaaga
     5941 tgacgaaagc attgtaaaga ttgatcaaaa gaaccacgag gacaattact tgaaaaataa
     6001 aggtcacttt ttttggagac aaaaccctgc taaatcttgc tccccacaaa cctccaataa
     6061 cagcagcggg gataacataa aacaacatag ataaatcaaa gcgcataaag ctacttgtta
     6121 aagcaatcgt taccaatttt gccagttgag aaaagaaaat cgtagataga gaatatagtg
     6181 ttgcttcttt tattggcaat gcaaacatca gcatcaacaa agaaacattg actggtccac
     6241 caccaatacc taaaaaacta gctaaaaagc ccagaacccc cccacatata aggtaccaag
     6301 tccatgacgt caaatgaaat tttggctgat gatgcttggt gtagaagaac gcaaaaacca
     6361 aggtcaatac cgtcaaaaaa atttggatca gctgcacatg cttctcgttt tcaaataact
     6421 gtaaaaaaac ttcgaataca atgttgccga ctactcctcc aacaacagct ccactagata
     6481 cccaaccgac aatctgccaa ttcagtgatt tcccgcttga tacttgtcgc acagtcgaaa
     6541 caatggacat cgtgaatacc gcaactgttg agtaaaaaga aatccctgca acagaatggg
     6601 cgccgataag atcaagaatc ggtttgatca acacgcctcc acccatcccg gaaatagcgc
     6661 caactgtgtt cgccagaacg atcaccacaa aataaatcag accaatcatc ttgtatttcc
     6721 tttctcttgt ttatatcttt tagcgattgt tactcttttc ttttattata cagtaaaaga
     6781 aacaaacgag gcttcgaaag cttttctact ttttaaggaa atttagtgct aaacgaaaag
     6841 aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaaacaaa aatagctcct
     6901 cataattttc gtttggttga acttattacc gcagatgcaa gcttttgaac accgtttaag
     6961 aaagaggtcg tgagcctgcc gtt
//
LOCUS       DANKHL010000150         6887 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000150 DANKHL010000000
VERSION     DANKHL010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.156"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            25..696
                     /locus_tag="PW101_002175"
     CDS             25..696
                     /locus_tag="PW101_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2514565.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="PW101_002176"
     CDS             755..1195
                     /locus_tag="PW101_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514566.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="PW101_002177"
     CDS             1207..1875
                     /locus_tag="PW101_002177"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL2514567.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="PW101_002178"
     CDS             1939..2562
                     /locus_tag="PW101_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDL2514568.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="PW101_002179"
     CDS             2618..3004
                     /locus_tag="PW101_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HDL2514569.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="PW101_002180"
     CDS             3217..4608
                     /locus_tag="PW101_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDL2514570.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            4955..5602
                     /locus_tag="PW101_002181"
     CDS             4955..5602
                     /locus_tag="PW101_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL2514571.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    5731..5947
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5995..>6887
                     /locus_tag="PW101_002182"
     CDS             5995..>6887
                     /locus_tag="PW101_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HDL2514572.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRV"
BASE COUNT         2302 a         1161 c         1460 g         1964 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgttttattt
     4861 gtctcacacc cttttccttc ctacttgtaa ccttgctccc gtgttagaaa catgttacac
     4921 taaatatgtg aaattattaa caaggagcgt cgttatgcga gaaaaaacag atacactgcc
     4981 aaaagcaact gcgaaaagat tacctttata tttacgctac ttgaagatgc tggatgactc
     5041 aggtataagt agaatcaaat caaatgagtt cagcgaaatc acccaaattc cttctgcaac
     5101 gatccgcaga gacttctcgc aattaggcga gctaggcaga agcggatatg gctatgatgt
     5161 cccttttctt atcgatgtat tcaataatat ccttaatact aaagaagaaa aacgaatcgc
     5221 attagtagga tatggaaatc ttggcaaagc attgaaacac aacaatttta gaagaaatga
     5281 aaatctaaat atcgtgtgcg tctttgataa tgatccagca ctgatcaatc gagtaattga
     5341 tggagaaatg atctatccga ttgatcgttt cgcggagata gccaaagcaa aaaatgtgac
     5401 agtagcaatc tctacggtac caagtaaata ttcacaatct gctattgatg aaattgtcaa
     5461 aggaaatgta accgctatac ttaattttgc gcctgacaga gtaactgtcc cagcttatgt
     5521 caatgttcaa tacattgatt tgacaaccga gttgcagacg cttatttact ttgatgagaa
     5581 ttactcagaa gttttttctt aggaaaattt gagtaggctt attgatcttg cgagaactcg
     5641 tcgtaattct ttcaaaaatt ttttagtact aaatgaagtg gttttttcac gaccacttgt
     5701 tgactaaaaa aaaaaaccat tgtagaatga agcataacgc tgaacaagag gagtaactgg
     5761 cacattcatt cttagagaga gaactatctg ctgaaaggtt ctcaatgaca accagtgaag
     5821 gtagcttggg agtttctgtc ttgaatggag tagagataga cgagtcgtga tcgttaactc
     5881 acttgagagg caataatgat tgattattgc aatttaggtg gtaccgcgta aatcatacgt
     5941 cctagaatac gaaagtattt taggacgttt tttagtttaa aggaggaaaa cagcatgacg
     6001 gaagaaaata acctatcaac aaaatataat ccgcaagaag ttgaagctgg acgttatcag
     6061 cattggttag aacaagattt gtttaaaccg agcggagata aaaaagcaaa accttattca
     6121 atcgtaatcc cgcccccaaa tgttactgga aaacttcatt tagggcatgc ttgggatacg
     6181 accttgcaag atatgctgat tcgtcaaaag agaatgcaag gatatgatac tttatggctg
     6241 cctggtatgg accatgcagg aatcgcgaca caagcaaaag tagaagcgaa gcttagagag
     6301 caagggattt ctcgttatga tctaggtcgt gaaaagttca tcgatcaagt gtgggaatgg
     6361 aaagaagagt atgcgagcca catccgcgaa cagtgggcta aaatgggctt atctttagat
     6421 tacagtaggg aacgttttac attagatgat ggattatctg aagcagtacg taaagttttt
     6481 gttaccttat acgaaaaagg tttgatttat cgtggtgaat atatcatcaa ctgggatcct
     6541 caagcacgaa cagctctatc tgatatcgaa gttattcata aagatatcga aggcgccttt
     6601 tatcatatga gctatgaact agcagatgga tcaggcgtag tagaaattgc gacaacccgt
     6661 ccagaaacga tgttgggaga tacggcgatt gctgttcatc cagaagacga gcgttatcaa
     6721 gcgctcatcg gcaaaaaagt gattctacct ttagtcaata aacaaatccc aatcattgca
     6781 gatgaatatg tagatatgga attcgggaca ggtgtagtaa aaatcactcc agcacatgat
     6841 ccaaacgact ttgaggtagg taatcgtcat gatcttccac gcgtaaa
//
LOCUS       DANKHL010000151         6818 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000151 DANKHL010000000
VERSION     DANKHL010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.157"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            933..1607
                     /locus_tag="PW101_002183"
     CDS             933..1607
                     /locus_tag="PW101_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2514573.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            1604..1945
                     /locus_tag="PW101_002184"
     CDS             1604..1945
                     /locus_tag="PW101_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514574.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            1975..2334
                     /locus_tag="PW101_002185"
     CDS             1975..2334
                     /locus_tag="PW101_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDL2514575.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            2859..4943
                     /gene="cadA"
                     /locus_tag="PW101_002186"
     CDS             2859..4943
                     /gene="cadA"
                     /locus_tag="PW101_002186"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL2514576.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            5234..6700
                     /locus_tag="PW101_002187"
     CDS             5234..6700
                     /locus_tag="PW101_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2514577.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2257 a         1103 c         1459 g         1999 t
ORIGIN      
        1 ggaagaggtt gtgaaatcag tgccttgacc tgagtattag agaaacaaaa cggaaaaata
       61 gcttctcata ttttaccgtt tttttagcta atatcgaggg tcagctgatt gaacaccgtt
      121 tatatcaaga ggttgtgcgc ctgccgttca gctccgagtc acaaggagta atttcaaaaa
      181 acagtttttc atgtttgatg actattgtga cttgtgaccg aggagttggc cggagagcaa
      241 cgtttattta aaaaaaagaa ctgcgaactc tcgcagttct ttttttaaaa tacgggataa
      301 aggaagtttc cagcagacaa gagaagaaag caaaagaaat tgctatttga ttcacaaggt
      361 tctataacga aatcgcaatc aatcaaacat atacaaagaa ggttattcaa atggaaatct
      421 gatttgatga tgcttctgct gttgaaataa agcagcatct agtacctgaa aacaaactaa
      481 tagtaacatt cgaagatgga gtaggtccgt attttcagca tgcaatgatc catatgtaag
      541 tttaattcat ccttgaaatc atcggaccag ataaagttat gaccaatcta tcccaagcaa
      601 tctagaagag atattagtaa aagaacattt actggaagtc ttagatgagc ctataaatat
      661 atgttctgat ggcgtcatca gtggtctgat tgacggaagc ttaggtgtga tcgatttctc
      721 tggaccaaac aacgaaggaa tcaaggggaa tccggcacgt taagaaaaaa agtataaaaa
      781 actagacact tgatgagcgt tcattcaatt gattgaaggg actaggctgg tgtcattttt
      841 tatacatata ttttctcttt cgtcttgata gggcttatgg gataatataa ggaagaaaat
      901 catcgtttta ttcaaaaagg agtttttgac aaatgaaaaa acgtatatta tttatcggtg
      961 acagcattac agacgcagga agagaccgag cagaagcaac tgatctaggt aaaggttatc
     1021 cacttttgat tgccggaaaa ctaatctccc aatatccaac gatgaaattt gaatgcatga
     1081 accgtgggat aggaggagac aaaattgcag atctgcaagt acgctggcaa gaagattgtt
     1141 tagctttaca acctgactat gtatcgattt tgatcggaat caatgatacc tggcataata
     1201 ctagcgatca ggctatattc ggaactgaga aatctgcgca gcaatttgaa tccgtttatc
     1261 gaaaattact aaatgaactt caaacagcgg aaatctgcaa catcatcttg atggagccgt
     1321 ttgtacttcc tataaccgac gaccgtcatg gctggcgtaa agacttagac cctaaaatcc
     1381 aaattgttcg taaacttgct aaggaatatg gcgcattgct ggttccttta gatggcatat
     1441 tcaatgcttt agggattgct tatggttttt cgaaatatac aagagaagat ggggtccacc
     1501 caacgattgg cggacatgag atcattgcac aaagctggat agatgcaatt gccgcagtcg
     1561 atttttttag tggagataaa aatgagcgaa agaaaagtga gaaatgatgg aaatacggtg
     1621 ggcagtaaaa gaagatttac ctcaaatcaa tcagctgatt actgatcatc cagaatcatt
     1681 cgtaaaagca aaaacggcag ctgacctaaa tgataagaat aaactattgc tcgttgctgt
     1741 aacaaaagaa aaacggatta tcggatttgc gcaagcggac gtgcaacagt cagaagaacg
     1801 ggtggatcag cgaccatcag gaaaactaat ccaacacaca ttagtaacaa aagaaaatac
     1861 ggaagaaatc caaaaaaaat ttgcagaata cttgatgctg gaagctaaaa aaagaagaat
     1921 aaaatcaatt tttgcccagt tttaatcaaa aacagtaata gaaggagaaa tacaatgagt
     1981 tgtttaggaa atgtcatctg gtttatattc ggcggactaa tcggtggtct tagctggtta
     2041 gcagcgggga tactttggtg tatcacgatc ataggtatcc caatcggtat gcaatgtttt
     2101 aagatcgcct ccctttcttt tgcgcctttt ggcaaagaag taatttatca ccagtctggc
     2161 atgtcattga tcgttaatat catctggctg atcgtttcag gcctaccaat tgctctcgca
     2221 catgtcgtca gcggcatcct tctatgtatc acgatcatag ggataccttt tgccaagcaa
     2281 tctttcaaac ttgctcgatt ggcgttgatg ccattcggtg cagaaattgt ataaaaagag
     2341 aataataaag gttagaaaac gcatagagtc aggtgggata catgtatttc acctgactct
     2401 atgtgttttc ctgtataagt tctcccatgt gacttttacg tcttttattt atatgggatt
     2461 actgtttatg gaaagttgaa ataattttca ttaaagaact ctttctatat ttttgaaagc
     2521 gtagtggagc ttcgaatatc tgggaaggac attttgcagg gattgaaaaa agatattgtc
     2581 ctatcgaaaa attaatgaat ttagacaaat tgccagcaac aggcttcaaa cttagttgct
     2641 atcctgtcaa gatcaagaag gcaagtgcgg gttggataag agcaggggca atgatcgaag
     2701 agaaaaagaa agaataagcc aaaacatctg tatggaagaa attttccgat gcagatgttt
     2761 ttcatttttt ctttcggata ccttgacata tgaataacca ttcatttata attagttatg
     2821 aagagatgtt catatgttga ggcgaggagg tcattaaaat gaaaagttac agattagaag
     2881 gtttagattg tgcgaactgt gcaatgaaaa tcgaaaaagg agttcagaag atcaatggag
     2941 tcaaggaagc aacggtaaac ttcacttctg gaaaactaac gattgacgca gaggaagatc
     3001 atttggctac gattgaacaa gaaacaaaaa aagtagtgaa ggaattagaa ccagatgtca
     3061 aagtaacaga aatagataag gaaaaagtat ctgaacatgg aaatgaaaaa gagagaaata
     3121 cgctgttccg gattttattt tcccttgcag gtatcgcttt attgctatta tttgatttta
     3181 atgaaccaat tcgcttgata gggtatttat tgatttatct gctgatagga tatgatgtag
     3241 tgaagaaggc agtgatgaat atcgtcaaag gaaaaatctt tgatgagaat tttcttatgt
     3301 ctgttgctac gattggcgct atgttgatcg gtgaatatcc agaagccgta gcagtgatgc
     3361 tattttatca aatcggcgag tatttccaag gattggcagt cagtcattca agaaaatcga
     3421 ttcgcgaatt gatggcgatc cgtccagaaa cagctcacgt gcagacgacg gaaggattga
     3481 tgaccgtgaa ccctgaagac gtgcagatcg gtcagtttgt acttgtgaag cctggtgaac
     3541 gtgtaccgtt agatggaacg atcatcgaag gagaatcatt agtcgatacg tcagcactta
     3601 caggagaatc agtccctaaa tctgtatatg ttggtgaaac agtattaagc ggatttatca
     3661 ataaaaataa accattttta gttcaagtag aaaaatctta tgaaaattct actatcagca
     3721 aattattaga attagtagaa aatgccagca gtaaaaaagc accagctgaa aatttcatta
     3781 ctaaattcgc gcgctattac acaccagtcg ttgtaggatt ggccgtttta ttagctgttt
     3841 tacctccact tgttgtttct ggagcggcgt tttctgaatg gatctatcga gcattgacat
     3901 ttttagtgat ctcatgtcca tgtgcattag tgatttccgt gccacttagt ttctttggcg
     3961 gtatcggagg cgcaagtaaa atcggtgtat tagtaaaagg ttcaaattac ttggagttgc
     4021 ttgcgcaaac cgagacagtg gtattcgata aaaccggcac attaacgaaa ggtgactttt
     4081 cgatccagac gatcgataca aaaatcgatc caaagaaatt catgcaatat attgcgagtg
     4141 cagaacagtt ttctacacat ccgattgctc aatctgtttt agaaggctac gcaggtccgc
     4201 ttttaccaac aactaatatc caggaatttg caggtgaagg gatcttagct gaagttgatg
     4261 gcaaacaagt attagcaggt aatcacaaat tgatggaaag atttgaaatc tcttttcctt
     4321 taagtcaaga aatcggtaca ttgctttatc tagcgatcga tcaaagttac agtggttatc
     4381 ttgttattgc agataccttg aaagaagatg ccgtagacgc attggttcaa ttaaaacagg
     4441 ctggtgtgaa gaacacagtc atgttgacgg gcgactcgaa aaaaattgct gatcatatcg
     4501 ggaagcaagt aggcgtggac aagatatata gcgaattgct tcctgaagat aaagtgcagc
     4561 ggttagagga aatcttacaa agaaacaata aaaagaccgc atttgtcgga gatggaatca
     4621 acgatgcacc ggtgctagca agagcagatg taggaatcgc aatgggtgga ttgggaagcg
     4681 atgctgcaat tgaagcagca gatgtagtca tcatgaatga tcaaccatcg aagattgcag
     4741 aagcaatcca tttagcaaag aaaacattga aaatcgtcaa acaaaacatt gtctttgcta
     4801 ttgggataaa aattttggta cttgccttag gtgcttttgg ttttgcatca atgggagatg
     4861 cagtatttgc agatgttggg gtgacagtat tagctgtttt gaatgccatg cgaagcttgc
     4921 atgtaaaaaa agaggacgaa tgaacgtcct ctttttttta tatccttttc ttaatgaaaa
     4981 atgtaaagaa aatagatagg attataaaat aactaatgtt tttaattttg cagaaaattc
     5041 aaaatagctt gaaattcaat aaggtcaatg aaataaaaga aaataactta tttttttgat
     5101 ggaaaaaata tgaaaaaacg atgtgtatta aataaaagaa tacacacata aaataacact
     5161 gtcttgtaag tttgttaaaa attgaatatg attacatctg gataacaaat ttttatcata
     5221 aaggagagaa cgaatgaaca acaaacaatt acgctggttt actgttgcgt taattgcatt
     5281 taacatggtt tggggcatgg ggaatgtagt taataactat gctcagcaag gagtatcagt
     5341 tgtcacctct tggctattga ttttggccat ttattttatt ccatacgcat tgatcgttgg
     5401 acaattagga tcagccttca aggatagtaa aggaggcgtc agttcatggg tcgaaaatac
     5461 gacatctaat aaacgactgg catactatgc cgcttggact tactgggtcg tccatattcc
     5521 ttatttggca caaaagcctc aagcaattct gattgctggt ggttgggcag tccaaggaaa
     5581 tggaaatatc gtaaatacga tgtcagtaca aatggtagca gttatttcac ttattatatt
     5641 tcttgccttt ttatatttat caacaaaagg actttctact ttgaaagtga ttggcggact
     5701 ggccggaaca gcgatgttcg tcatgtcgat cttatttatt cttttagctg taacggcacc
     5761 gttcatcaat ccaacgatgg aatttgccac accacatatg aatcaagtga aaacatatat
     5821 tccaacattt gatttcagtt atttcacaac cgtttcgatg ctagtatttg cagttggagg
     5881 ggcggaaaaa atatcccctt atgtcaactc tacaagaaat cctgcaaagg aatttcctaa
     5941 agggatgatc ttcttagcag caatggtagg gatttgtgct gtattaggct cagtcgcaat
     6001 gggtatgctg tttgctagtg ataatattcc taaagacttg atggcaaatg gggcatacag
     6061 tgcttttcaa attttaggta attactatgg tatcggtaac tcattgatga tcatttacgc
     6121 attgacaaac atgattggac aaatctccgc attagccttt tcaattgatg caccactaaa
     6181 aatcttgtta gcagatgcgg atccagaatt tgtaccaaac tggctacgta aacgtaatcg
     6241 taaaggtaca ttggtgaatg gttatcttct gacaggtata ctagtaggaa tcattatttt
     6301 gttgccgatt tttggtatca aagatatgaa tgaattagta aaatggttga cgaacttgaa
     6361 ctcagttgtc atgccaatgc gttacctatg ggtgttcttg gcatatatga tgttgaataa
     6421 agcttataag aaattcaatt ctgaatataa atttgttaaa aatccaaaaa tcggttttat
     6481 ctttggtgct tggtgctttg ccttcactgc gtttgcatgt atcctaggaa tggtgccaaa
     6541 agtagattat actgctgatc ctaaagcatg gtggttccaa ttatttacga atatcatcac
     6601 accaatcgtc ttgatcttat taggtatgat cttgccagca attgctcgtc gagacaaaaa
     6661 caaagaaatc actgaaccag aaacagtggt tcagccttaa aatagaaata cttgttacaa
     6721 gaggttgtga cagaagtggt cagcttcaag aaataagaag gaatttacga aaattgcttc
     6781 tcaaattttt gtgaagtttc ggcttgtttt tcgaagaa
//
LOCUS       DANKHL010000152         6702 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000152 DANKHL010000000
VERSION     DANKHL010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.158"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..608
                     /locus_tag="PW101_002188"
     CDS             <1..608
                     /locus_tag="PW101_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514578.1"
                     /translation="ENQLFAGDVLEIVLNDQAGEAAAKGVQNKPSTNDEIGNHNPSDT
                     PVVYHDAPPFAPATKIVVEGGLSFLAPSKLDCGQIKATGLTQEAFGQMDIQEKLWIYD
                     QRETKSPWILTIKLSQPFTTDAGFSMQDVLYFKKAEEYQLVNEEELEIVRGQNSTNVQ
                     TDYSEYLNNGRAFKLVLTKENQIAGNYSAEMTWTLADAPTE"
     gene            628..1656
                     /locus_tag="PW101_002189"
     CDS             628..1656
                     /locus_tag="PW101_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL2514579.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1653..2411
                     /locus_tag="PW101_002190"
     CDS             1653..2411
                     /locus_tag="PW101_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2514580.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2497..3237)
                     /locus_tag="PW101_002191"
     CDS             complement(2497..3237)
                     /locus_tag="PW101_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2514581.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3298..4551
                     /locus_tag="PW101_002192"
     CDS             3298..4551
                     /locus_tag="PW101_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2514582.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4852..5100
                     /locus_tag="PW101_002193"
                     /pseudo
     CDS             4852..5100
                     /locus_tag="PW101_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5222..5548
                     /locus_tag="PW101_002194"
     CDS             5222..5548
                     /locus_tag="PW101_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL2514583.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5616..6329
                     /locus_tag="PW101_002195"
     CDS             5616..6329
                     /locus_tag="PW101_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDL2514584.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6639..>6702
                     /locus_tag="PW101_002196"
     CDS             6639..>6702
                     /locus_tag="PW101_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDL2514585.1"
                     /translation="MKIDKILNNNVVISKNGFGEE"
BASE COUNT         2348 a         1110 c         1326 g         1918 t
ORIGIN      
        1 cagaaaatca actttttgca ggagatgtat tagaaattgt attaaacgac caagcaggag
       61 aagctgctgc taagggtgtg cagaacaagc caagtaccaa cgatgaaatt gggaatcaca
      121 atccaagtga tacgccagtt gtttatcatg atgccccgcc ttttgcacct gctacgaaga
      181 ttgtagtaga aggtggtctc tcatttcttg ccccttccaa actcgattgt ggtcagatca
      241 aagcaactgg gttgacgcaa gaagcatttg gccaaatgga tatacaggaa aaattatgga
      301 tttatgatca gcgagaaaca aaaagtccat ggatcttaac gataaaatta tctcagcctt
      361 tcacaaccga tgcaggattt tctatgcagg atgtcctata tttcaagaaa gctgaggagt
      421 atcaattagt gaatgaagaa gaactggaaa ttgtcagagg acaaaattca acgaatgtgc
      481 aaacagatta ttcagaatat ttaaataatg gtcgggcgtt taaacttgtc ttaaccaaag
      541 aaaatcagat agctggtaat tactcagcag agatgacatg gacattagca gatgcgccga
      601 cagaataaag atcaaggaga atgattgatg aatcgaatat ggaataaaag tcatcgaaac
      661 aactgctctt tacttctgat ccttctcctt ggattcatcc tttggcagac acctgtccat
      721 gcctccgaat tcaattttgc ggtagatttt gaattaccgg ctaatcagat tgataagaac
      781 aaaacatata taaatgtaag gatggagcct gggaaaacac aggaaatccc ctacacggtc
      841 tttaaccgaa cagataaatc agtaacagtt gagacattga tccactccgc tacgacgaat
      901 tcaaacggtg tgatcgaata tggagaatca aaaagtaaat ttgatgaaac attaagttat
      961 gatatgaaaa acttagcaaa aacagtggga gaaataactg tccctcctca aagtgcggtc
     1021 actgagacac tttcactgaa agctccagaa aaagagctgg ctggtgtggt cgcaggagga
     1081 atcacgtttc aagaaaaaaa agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat
     1141 cagttttctc aagtgaaagc aatcttgatt cgaaatagcg aaaaagagtc ttccccaaat
     1201 ttgaaattaa aaaaaactta tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat
     1261 cttcaaaatc caaatgcttc ttatatgttc ggcttgacta catcggttga aatctacaaa
     1321 aaagaaacaa aagagcctta tctttcaata aaaaaagaaa aaatagatgt agcacccaat
     1381 acaaattttg atttacttgt cccattagaa ggaaaaaaat tagctccggg acattatcaa
     1441 gcggttgtga agggtgcttg gaaagaaaaa cgctggaatc tccataccga ttttgaaata
     1501 actgatgatg cagcggacca attgaatgaa aaggatattg atttaaaaaa tcagccagaa
     1561 acatttgact ggcgatggct gatacttgtt ggagtcattc ttattggctt atttgctgga
     1621 ttatttattt taaaagaaag aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg
     1681 ttctagctgg aagtctatta ttatgtacac aaagtgtttt tgctgagaat aagagctcag
     1741 ccactacaga aggaacgatt tctgtaacaa aaccagatgc cgcaaatcct gaagacgttg
     1801 ttccagttcc tcctgttgat ccaaatgatc ccgatacgcc gtttacacct aacgaaccaa
     1861 atcagggaac atccggatta ctgacgattg accaagctcc agatcttgat tttggaacaa
     1921 tcaagttagg tacagcaaaa acagtatatg ctgcattaca aaacggaacg gacagtacag
     1981 ggacagcaac acaagtacag aatttcgtac aagtaacgga taaaagcgga atttatgccg
     2041 gttggaaact atcagtcaaa cgaacagaat ttattggtac aacatctgat acgaataaac
     2101 taactggttc aaaggtgaaa ttcaaaaacg ccgtgattcg aaatagcgca actaatagca
     2161 gtggaacgcc atctactatt tctggtactg gcgctgaagg aattgagatc ccagcagatc
     2221 aggcagtaga tctagtaact gcaggagcaa atgaagggat tggcacatgg atttattcat
     2281 taggagagaa tgtggaacaa gggagacaat cagtggaatt atttgttcct gtatccaatt
     2341 atgccgtaga caactacgtt tcaacattca catggtcgtt aacagatgct cctacagaag
     2401 ggaaagcgta aaatcaaatc aatgacttca gggagtggag ccgattcaag gccaactccc
     2461 tgaaatctgc tatgcttacg tctcagctct tattcatcaa ctcgaagcag ttttttggaa
     2521 gtcatggaaa cgttcctttg tcttttcaaa gatctttcga ccaacgagta aggagtcacc
     2581 attgaaaaat tctaacacat aatgagcaga atcaattttc tgcaattgcg ataagttgat
     2641 acagtaagat tttaatagta aaaagtaagc atacttttcc aactctgtct tcaattgttt
     2701 taaagaagta ttcactaatt tatcttctgt agttgttttg accataactt ggtttttcaa
     2761 acctttgata ctttcaatat ataaaatttc ccgaggatac aggacgagac ttccaccttt
     2821 ttgatcaata aagattttat cgacatttac gatttgctgt tctttccaat tgatctgttc
     2881 tagcacttga cgaatttgtt cagataaatt cctgtctttt ctgatatatc caatcggaag
     2941 gattttccga ttgatgatct ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt
     3001 actatgtgca ttttttttac gtatttgttc ggcgacatca atacctgtga gtgattgctt
     3061 caagtcgata tcgataaaga aaacatcgtg attatcaata gtacccaact ggatcaactg
     3121 attgatttga gagatagaag tgagctgcac gatattctcg atatgatgat tgaccaattc
     3181 tttgatgatc cattcgctgt gaaactggtt atcttctatg ataaaatact ttttcatatg
     3241 caggctccta ttaaaaaaat atgtgtacat tttgaaacaa aacaatggag gaataagatg
     3301 gtcgcaacag tgacacttta tttgaccttt actttgattt tagatttagg tatctattta
     3361 ttcttttcga aacaactgct agtaaaaaac agctttggtt ttatagcttt gatggtggtg
     3421 acacttttat ttcctatccc taaagtgagc tttccttacc tagttggctt attattgatg
     3481 tttctttttt attttactac gaaaaacctg tttatcagct gtttgatcag taatcttttt
     3541 tttgctatca ctggactcag cttttttctg gtttttgatc tgatggatta tttaggaaag
     3601 agacatcttg gaacagcaag tttttttgca ctacttatcg catttatttt ctatctatta
     3661 gtaaaaagta tagacaaaca gacgagattt ctcaacttgc tttttactta tccaactgaa
     3721 cactattttg gaggtatttg tattttatta agctggggcg tgatttactt ggtattggga
     3781 agtgttcact tccgctcatc taattatctg cttctatctc ttttgtcgat agtcatcagt
     3841 tttttttctt tgtttttttc cattatgttg atcagtttcc aagtaaaaga aagacaacat
     3901 caggaatcgc ttcaactgta tcaaataaat gaagaatatt accatcatct agaggaattc
     3961 aaacatgatt ataaaagtct actgttttca ttaaaaatta ctcttcagga aaataaaaaa
     4021 gaaacgttgg aatttttaga aatcttagaa gatcattctg cagctatttt tgaaaaaccg
     4081 caaatacaac agctaacaaa tattgtatcc ccagctgtca ggggtgtctt tttaaaattt
     4141 ctagaaaaag cagaatcgga gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa
     4201 acaatcccga ttgatctggt tgtttttatt cgctgtttat ctatttttat ttctaacgca
     4261 ttcgatcatc gtgtagccga tcagagtccg attacgatca atctgctgga agaaaaagag
     4321 ggattacgtt ttagtatagc gaacaaggca aatccgacga atttgacgct atcggagatg
     4381 gtcaaaagag gaaaaacctc taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa
     4441 atgttggatg tttacgaaaa tgcagaacta aaaatcgaat tttcaaaaca gaatcactat
     4501 ttttttgtag aaatgtatct ggcatctaga aagagcaaat cagcctactg acttactgtc
     4561 aaaggataag cttgaggaaa gtctacacgg agatagagac aacaacacag catattcaaa
     4621 agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc ttttgctatg tagttaaaca
     4681 aagtagttga cacttccgct acttagtatt agtacgaaag tatacttcat gatactaagt
     4741 agataagaag tgaaaagcgg aacccacgat cgtcaaaatg ctaactgctt tgcttacacc
     4801 agataaaggg aagagcaata tcgcttattt taggcgtgga gaagcaagaa aatccctacg
     4861 atcacacaaa aaatgctgac ccagcagtta agagagttag aacaagatgg cattgtccat
     4921 cgaaaagtct ataaccaagt cccgcctaaa gtagagtact cgctcaccga agaaggaaaa
     4981 tcattaagag agattctagt agcgatgtct gtttggggag aagaacgtat cgaaaaacaa
     5041 aaagcctcag gggaagaagt cacattactg catacacatg gtggctacct aaattactga
     5101 tttctatgac atttatttta aataaaagaa aacaggagtt tttttaaaag catcatctat
     5161 tttttatgta aaaaaatggt atgattaatc aagtaagaaa tgattggaag aaggcaagca
     5221 aatgaatagc atggaaaaag ttaaccagtt tagagatgaa cgtaattggc gacaatttca
     5281 taacgaaaaa gatttggcta tttctatttc tttggaagca tctgaattgt tagaactttt
     5341 tcaatggaaa acgccggaag aagtaaaaga aacacaatta gaacgaatca aagaagaact
     5401 ggcggatgta ttgatctatt catacatgat ggcagacaac cttcagctgg atctagatga
     5461 gatcattgaa gaaaaattga tcaaaaataa tttgaaatat cctgtagaaa aaagcgcagg
     5521 tgtacgaaaa aaatacacag agttttagaa atgtgtcatt cggctgttgg aaagagcaaa
     5581 agaaaaacag taaaatgagt ctggagggag agaaaatgaa aaaaaaagga aaagcaaaaa
     5641 gatggctagt caatatcctc ttgttcctct tgttattagt aggactagct ttgattttca
     5701 atgaacagat caaggattat cttgtcagag agacaggaga taaatatgct atagcgaatg
     5761 taacaaaaga agatctgaaa aagaataacg ataaagatgt cagttttgac tttgatgcgg
     5821 tagaaccaat gacgacagaa ggggtcatga ggtcgcagat gagaggaaca gatctaccag
     5881 tgattgctag tatcgcagtc ccttcagttt caatcaattt accgattttc aaaggcttgg
     5941 ataatacgtc actactttac ggagcaggaa cattatctcc agatcaagaa atgggaaaag
     6001 gaaattatgc cttagcaagc catcgtgcaa cgaaccctga attgttgttt acaccgctgg
     6061 aaaatttaga gatgggtgcc aagatctatt tgacagattt agaaaatgtg tatacataca
     6121 aaacattttt taaagaaaaa gttgctccta cagataccca attactgaat gaagtcgagg
     6181 aaaaagaaat tgtcacacta attacttgtg gcgacatgga cgccgtcacg cgactagttg
     6241 tacaaggaga actggaaagt gttacaccga taaaagatgc gacagatgat atgagatctg
     6301 ctttcaatct agagacaaaa acattttagg atactgggac ataagtcgtt acagattaaa
     6361 aacagccaaa ggatatgaat cgataaggaa aaaccaattt gctgaaaaaa agaggaacgc
     6421 aaagaggtgc atctctgatt tggttgtttt ctagatagac ttgtgttcca gtttttttat
     6481 tttttatatg aaaaggcttg cataaataaa aattcgatgg tatgatgatt ttgaataaga
     6541 aatggattgt tactgtacgg caggcaaaac cagaattcag ctaagaaaca gtaggcgaat
     6601 tctaggtttt tttatttttc ttgagagggc gaagcaaaat gaaaattgat aaaattttga
     6661 ataacaatgt cgtcatttcg aaaaatggat tcggggaaga ag
//
LOCUS       DANKHL010000153         6645 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000153 DANKHL010000000
VERSION     DANKHL010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.159"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(70..390)
                     /locus_tag="PW101_002197"
     CDS             complement(70..390)
                     /locus_tag="PW101_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDL2514586.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(538..2772)
                     /locus_tag="PW101_002198"
     CDS             complement(538..2772)
                     /locus_tag="PW101_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2514587.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3251..3517
                     /locus_tag="PW101_002199"
     CDS             3251..3517
                     /locus_tag="PW101_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDL2514588.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3517..5244
                     /gene="ptsP"
                     /locus_tag="PW101_002200"
     CDS             3517..5244
                     /gene="ptsP"
                     /locus_tag="PW101_002200"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDL2514589.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            5408..6454
                     /locus_tag="PW101_002201"
     CDS             5408..6454
                     /locus_tag="PW101_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2514590.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6471..>6645
                     /locus_tag="PW101_002202"
     CDS             6471..>6645
                     /locus_tag="PW101_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2514591.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSV"
BASE COUNT         2066 a         1215 c         1349 g         2015 t
ORIGIN      
        1 ttagagcgtg agacaaaagt gttctttact tttgtcccac gctctttaat tattatgttc
       61 aaaatttgtt tacacggcta atgtagcgcc taattcatgt tttggaatcg agatttcaac
      121 aaggcctgcc aattcgatta ctttgatttc atcctcgtgg taaacggcgt ctttgatttt
      181 atctaagaaa taatttttag catcttcgat ttctttcggc tggattttac gtttgtcatt
      241 gatcagcaga atttcattat gttcaggggc ttcagtgtag acaatagtgg tatttccggc
      301 tgtgtagacc ttgactagat gggcatcagt gttttcaagc tggtttaata caagacggga
      361 atggctgttt gttacattta ccaatttcat ggcgctcacc tcttttatgt atttttaatt
      421 gcttgtttct cttattataa tttctttttt ataaaatgaa cagtattttg acttttctga
      481 aaaaagaatg agacggcatt tctcaaagaa ataccgtctc acgctataaa actggattta
      541 ttcagccgtt tcttcggctg attctttagc caaacgttct ggtttagaag taacgatgat
      601 ttttccatct ttatccagtt ttgctttcaa ctcatggata gaagggtgat caagataaaa
      661 ttcagcgata ccatcttcga tttgttcttg gatcgtgcgt cgaagaggac gagcacccat
      721 agatggatca tagcctaagt caactaattt ttctttgaca ttcgtaggaa cttcgatgtg
      781 aagttgctgt gctgccagca tttggttgac atcgtctagc attagtgtga cgattgtcat
      841 caagttttct tttgataatg cactgaattc gatgatcccg tcaaaacggt tcagaaattc
      901 tggtgtaaag tagttgttta attggttcaa tacagaacga gtaacacctt cgcgtgccgc
      961 accaaaacca acgtttgctt ctactttacc agttccggca ttacttgtca taatgatgat
     1021 cgtatcttta aagctgaccg ttcttccttg tgcatctgtc aatcgaccat catctaagat
     1081 ttgcaagaac atatgcaaaa catctggatg agctttttct acttcatcta acaagacaag
     1141 actgtaagga tttctccgta ctttttctgt taattgacca gcttcttcgt atccgacata
     1201 acctggagga gaaccaatca atttagagac actatgtttt tccatatatt cagacatatc
     1261 gaaacgaatc attgaatctt gtgaaccgaa tagttcgtag gcaagttgtt tcgcaagctc
     1321 tgttttacct acaccagttg gtccaacaaa taagaaggag ccgattggac gattctgttt
     1381 gtttaatcca acacggtttc ggcgaatcgc tttcgctact cgatctacag cgttatcttg
     1441 tccgatgaca tgagctttca agtcatctgc taagtttttc agttgtgtct gttctttttc
     1501 ttttaattca cccactggaa tacctgtccg ttgttcaacg attttttcca tatctttttc
     1561 ggtaatcaca ggtgtttcct cttcagtgag ctgacgttct ttcatttttt gaagtttatt
     1621 gatctgatcg cgataataag ctgctttttc gaaatcttct tctcgagaag ctaaaacttt
     1681 ttgttcttct gcttcttgca gttttttctc gattgtttta ggatcaacga tttggatcgt
     1741 caagtttttc ttagaacctg tttcatctag caaatcgatg gctttatccg gcaagaagcg
     1801 atcttggacg taacgattgg ataaagttgc ggctgcttcg atggcctcat cagtatattt
     1861 tacatgatga tagtcttcat aacgtttttg caaacctttc aaaatactga tcgtttcttc
     1921 gacagttggt tcatctaccc gtacaggttg catacgacgt tctaaagcag cgtctttttc
     1981 gatgatgcgg tactcattta aggtagtagc accaaccatt tgcaattctc ctcgagctaa
     2041 agctggtttt aaaatattac cagcatccat atttccatca ccagcagaac cagcaccgac
     2101 gatttcgtga acttcatcta tgaataggat cacattttct gcttgacgga tctcttcaat
     2161 caatttttgc atacgttctt caaattgtcc tcgtatacca gtaccttgga ctaaagatac
     2221 gacatctaaa cgaatgactt ctttgtccat taatttttgc ggtacatcac catctacgat
     2281 tttttgtgct aaaccttcaa caactgctgt tttaccaaca cctggttctc caatcaagac
     2341 aggattgttc ttggttcggc gatttaaaat ttcaatgact cgtttgattt cgtcatcgcg
     2401 tccgatcaca ggatcgatat caccttgtcg tgcttgttct gtgatattga ttccgtattc
     2461 tcccaataaa ccttctgtat tgccttgagg tggctgacca ccattgaagt tgttgtttcc
     2521 gccaaattgt gttggtggtg tttgctcata atttccttgc tgttgcattt ggcgagacat
     2581 ggaacggaaa agatcgtcca aactgccaaa tccaaatgga tcattttggg ccatattagt
     2641 cagacctcca ttttgttgat ttttgatttt ttgataacag ctctgacagt aatcgagttg
     2701 tgtccgctga ccattgacag tggcatacaa gtgaatcgtt gcctcattct tgccgcagtt
     2761 ttgacaaagc atgaatcatt cacgtccttt cacaaaaatg ggtacatcca tattttacat
     2821 ctcgtttttc atgaggtaaa agaataaccc ctagtttttt aagagattta tgaggtagaa
     2881 aaggtgaaat ctctattgac cattcctgac ctttgactct tattatacta aaaataaaaa
     2941 gggaagcaag taattcgttt catatgaaaa caaagagggt caaaggaaag aatgataaac
     3001 gtcaggagaa gagaaagcga caagaaaaag gaaataaaca cgtttgttat aaaatgtgaa
     3061 gaaaaggatg aaaaagaatc cggaataatt tgaatacttt tatctgaaat tgaatgaaaa
     3121 ctgttgtatc taacgaaaaa aaatgttaat ctttacagat gaaagggtgg catttgttag
     3181 gattctctga attactaaaa aagaaaaccc tcacataaaa acaacactca cttaaaggag
     3241 accgattaat atggaaaaga aagaatttca cgtagtagca gaaactggga tccacgctcg
     3301 tccagctaca ctattagtac aaacagcaag caaatttaac tctgatgtaa acttagagta
     3361 taaaggtaaa tcagtaaact tgaaatcaat catgggcgtt atgtctttag gtgtaggtca
     3421 aggttctgat gttactatta ctgcagaagg tgctgacgaa gctgatgcaa tggcagcaat
     3481 cgttgaaaca atgaagaaag aaggcttatc tgaataatgg ttgaaatgct aaaagggatt
     3541 gccgctagtg acggtgtagc cgttgcaaaa gcttacctgc tagttcaacc ggatttaaca
     3601 ttcagtaagg caacagtaga agatactgcg gctgaagaag ctcgtttgga tgctgcatta
     3661 gctaaatcta ctgaagaatt gcagcaaatt cgtgaaaaag cagcgcaaag cttaggtgaa
     3721 gcagaagcgc aagtatttga tgcccatttg atggttcttt cagatccaga aatggtaggt
     3781 caaatcaagc aaaatattaa agacaacagc gtaaatgctg aatcagcgct aaaagaagta
     3841 acggatatgt atatcggtat gttcgaagca atggaagata atgcttatat gcaagagcgt
     3901 gctgcagata ttcgtgacgt tgctaaacgt attttagcgc acttattagg cgttacttta
     3961 ccaaatccat caatgatcaa cgaagaagtg gtcgttgttg cacatgactt gactccaagt
     4021 gatacagcac aattagatcg caattttgta aaagcgtttg taacagacat tggtggacgt
     4081 acttcacatt ctgctatcat ggcacgttct ttagaaattc cagcaatcgt tgggacaaaa
     4141 gaaattactg ctaaagtcaa agaaggcgat attttggctg tcaacgggat cgaaggcgat
     4201 gtaatcatcg atcctacaga tgaacaaaaa gctgaatttg aaaaagcagg tgcagattac
     4261 gcagcacaaa aagcagaatg ggaaaaatta aaaaatgcag aaactgtcac agctgacggc
     4321 aagcattttg aattagctgc aaacatcgga acaccaaaag atctagttgg tgtccataat
     4381 aatggtggag aagcagttgg tctgtaccgt actgaattcc tgtatatgga ttctccagac
     4441 ttcccaacag aagatgacca atatgaagct tacaaagctg tattagaagg tatggaagga
     4501 aaacctgtag ttgttcgtac aatggacatc ggtggagata aagaactacc atatctgcaa
     4561 ttacctcatg aaatgaatcc attcttagga tatcgtgcat tgcgtatcag tttatctgaa
     4621 caaggcgacg aaatgttccg tacacaaatg cgtgcattgc ttcgtgcatc tgttcatggt
     4681 aatttacgta tcatgttccc tatggtagct acactcaaag aattccgcgc agcaaaagcg
     4741 atctttgaag aagaaaaaca aaaattgata agtgaaggca aggaagtatc tgatacgatc
     4801 caagtcggca tcatgatcga gattccagca gcagcagtct tagcagacaa gtttgcaaaa
     4861 gaagtagatt tcttctctgt aggtacgaac gatttgattc aatacacaat ggcagcagac
     4921 cgtatgaacg aacgcgtttc ttacttgtac caaccatata acccatcaat tttacgtttg
     4981 atcaaaaatg taattgatgc agcacacgct gaaggaaaat gggcaggtat gtgtggagaa
     5041 atggctggag atcaaacagc tgttccattg ctagtaggta tgggtctaga tgagttctca
     5101 atgagtgcga catctattct taaaacgcgt agtttaatga aacgcttgga cacagcgaaa
     5161 atggctgaac ttgctgatcg tgcattgaaa gaatgcgata tgatggaaga agtcgttgaa
     5221 cttgttcatg aatatgtaaa ataaacaacg tatcaaaagc tggtgtggct tgtaaaagag
     5281 ctacgccagt ttttttgttc ttttttaaga aaacgttttt gtcagtaaga atagacaaaa
     5341 atagggctta agtttgagtg acagtttgta ggctatgtta tactatataa gtgaaggggg
     5401 acgtaaggtg aaggtattat tatattttga aagtgaaaag atgcttgcaa aatctggaat
     5461 cgggcgtgcg cttgatcatc aaaaacgtgc tctgacggaa gtcggtatct cttatacact
     5521 agatgaaaaa gaagattatg acatcctgca catcaataca tacggaatca atagtcataa
     5581 tatggtcaat aaagccagaa gagaaggaaa aaaagttgtc tatcatgcgc attctacaga
     5641 ggaagatttc cgaaattcct ttatcagatc gaaccagctt tctcctatcg taaaaaaata
     5701 tctggtagga ttgtaccaaa aagcggacta tctgatcacg ccaacacctt attcaaaaca
     5761 gttgttggaa agctatggta tccgtgtgcc gattcaagct atttccaatg ggatcgactt
     5821 agagaaatat caccctgatc caatgaaaga acaaaagttc agagaatatt tcaaactgtc
     5881 tccggatcaa aaagtaatca tttgtgtcgg tttgtttttc gaacgtaaag gaatcattga
     5941 tttcgtagaa attgctaaga agatgcccga atacacattt atttggtttg gtcatgttcc
     6001 catgtactcg attcctcgga acatccggaa aattgtaaaa gaagatcatc ctgaaaatgt
     6061 cctgtttcct ggttacattc gtggagagat catagaaggt gcgtattcag gtgcagatct
     6121 attctttttc ccttcttatg aagagacaga agggatcgtt gttttagaag ctttagcaag
     6181 caaacaaaac gtacttgttc gagatattcc agtgtataat ggctggttag aagatagcaa
     6241 gaactgctat atggggaaga caaacgaaga atttatccga ctgatccaac agattaccaa
     6301 tcaagaacta ccgagtacga cacaagccgg ttatcaaaca gcaaaagagc gaagtatcaa
     6361 taaaatcggt gaagaactta aaaatgtata cgaaagtgta ctgaatgaaa aagtctctag
     6421 caaattaaaa aaagtaaatc aggtcaagga ctagtttgga ggcgaaaaac atgaaaatcg
     6481 gcttttttac cgatacctac tttccgcaag tcagcggagt tgctacatcg atcaaaacat
     6541 taaaacaaga attagaaaga catgggcatg aagtctatat ttttacaacg accgatccta
     6601 atgcggataa gttggaaaaa gatgtgattc gaatgccaag tgtac
//
LOCUS       DANKHL010000154         6598 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000154 DANKHL010000000
VERSION     DANKHL010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.160"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(60..209)
                     /locus_tag="PW101_002203"
     CDS             complement(60..209)
                     /locus_tag="PW101_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514592.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
     gene            1177..1503
                     /locus_tag="PW101_002204"
     CDS             1177..1503
                     /locus_tag="PW101_002204"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2514593.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            1484..2419
                     /locus_tag="PW101_002205"
     CDS             1484..2419
                     /locus_tag="PW101_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL2514594.1"
                     /translation="MQKWICDLFMCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSE
                     KNRALNCLTVSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEE
                     IQAAVEGAISVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGL
                     DTQPITAIAILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKP
                     LLVQIANALLRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSA
                     KGYLEPKPAKEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            3048..3299
                     /locus_tag="PW101_002206"
     CDS             3048..3299
                     /locus_tag="PW101_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HDL2514595.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            3841..3999
                     /locus_tag="PW101_002207"
     CDS             3841..3999
                     /locus_tag="PW101_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2514596.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            4071..5297
                     /locus_tag="PW101_002208"
     CDS             4071..5297
                     /locus_tag="PW101_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2514597.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(5386..5778)
                     /gene="rpsI"
                     /locus_tag="PW101_002209"
     CDS             complement(5386..5778)
                     /gene="rpsI"
                     /locus_tag="PW101_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL2514598.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(5794..6237)
                     /gene="rplM"
                     /locus_tag="PW101_002210"
     CDS             complement(5794..6237)
                     /gene="rplM"
                     /locus_tag="PW101_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDL2514599.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLAGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
BASE COUNT         2132 a         1228 c         1157 g         2081 t
ORIGIN      
        1 atataaaagg tacgggttac tgattacttg taaatcttta cgttccagtt ttattctttc
       61 taaaaaatat actcacttct tttcttagac ttcttttttt gattctcctc tttatggtct
      121 tgtccttttt tgccttcaaa atatttaaat aaaccccttt caaaagcatt tctaggtttt
      181 ggacaataag gaatgacttt tttcttcata taaatacctc atttaactat ttgatatcat
      241 cacattatca aaaaaaatag gttttttatt aaaactatct tgacctttcg ggagatttca
      301 ctgtgattta tttttattcc catacttttt tcgttggtca ctttcgtata cctaatctaa
      361 gcgatcatcg gcgacggtgc ttttacaacg aatggcagga caaaacaagg ggcggaaaaa
      421 cttttttgcc tatttcataa aaaacttttt cctacagcaa tcccttgttt tgtcctacca
      481 cacgttgtga acccaaagca cgccgacgaa cgcttagcta tacgaaaatg acctccaaaa
      541 acaaaaaagt gaggtgaata gaaacaaaaa gaaattgaaa tctcccaaaa tgtcgtccgc
      601 ctcggagaca acttgctgct gaaaatggtt ggttaattca tttaagtatt aaataagata
      661 aatcatcaaa ttaatcggaa aaatagcatg attggcctta tcaaaaataa actgtaaaaa
      721 cttatatttc tatgagtggt ggaacaatgc taataccaaa ttaatttatc gattacccac
      781 ctgcacaaac caaagatagc attattagga aacgaatcaa tcagcgaatc cagcattata
      841 atgaggatac taaaaataaa gttttcataa ttataaatcc gccttcctac gaaaggcacc
      901 aatgaggtga tgaaaacctt agagcgcttt ctttcttttt ttattccgtg ttaccctttc
      961 cttgcaggtc cctggtacac tacagaaccc gaggaggtga gtggcggggc tgtatagcgg
     1021 caaccccgca taattatatg gtgtcggtca tccgttaagg catcaatgaa tggcgcaaac
     1081 ccgtagcccg taaacttatc tcttccgaag ttgactcgat ttcgccggat gaccagtccc
     1141 taccaaacct gtactatttt tttacaggag gttgctatgt tcaaaatctt tcgtaaaaat
     1201 tgttgtggct tggatgtcca caaaacgtgg atctatgcgt gtatcggaat cacagatgcg
     1261 aatggtcgta cagtgtataa gcaagctcgc ttctcttcct ttacaaaagg tctcaatgaa
     1321 ttagctgaat ggcttgctaa atacaactgt aaggatgtgt gtttggaatc ttccggtaaa
     1381 ttttggattc ctgtttttaa tattcttgaa aaaacttgtt ttgtcacact ggctcatccg
     1441 aagtacacca aaccccagaa aggaaataaa acagatcgca aagatgcaaa aatggatttg
     1501 tgatctcttt atgtgcgaca tgatcaaacc cagttttatt cctggtcttg aaatccgcca
     1561 gcttcgtgat ctaatgcgtt atcggacgaa actgaccaac atgaaaacaa gtgaaaagaa
     1621 tcgtgcttta aactgcttaa ccgtttctaa tctgaaacta gatgatgttt ttagtgatgt
     1681 gttcggaaaa tcatccactt ctatcatcca atacttgtta gaacatcccg gtgaatcatt
     1741 tgatgttcga ccatttatcc atgcccgttg taaaactccc gttgaagaaa tccaagcggc
     1801 agttgagggg gcgatttctg tacctcaagc gattaagctt cgccaatgcc tcaaccatat
     1861 taacgagatt gagaagcatc gagaagagat caatctagag attcaagctc ttgcagaacc
     1921 cttctcccct gttttggaac tacttcgtac cataccgggc ttagacactc agccaataac
     1981 agcgattgcg attctttcag aaataggacc ggatatgtcc gtgtttccaa cttcgaaaca
     2041 ctttatttct tgggctggat gctgtccaag aaacgatcgc agtgcccgac gaacaaaatc
     2101 aacacgtatc tctcgtgcag ggcagtcgtt gaaaccgcta cttgtacaga ttgccaatgc
     2161 tcttctcaga tccaaagaac atccagaatt taaaaatcgc tatcgtagaa tcaaagcgaa
     2221 tcgtggacac aagaaagcta tcattgctat ttgtaagatg ctcctgaccg ctatctggaa
     2281 tgtcttatca aaggtggaac cctattctgc taaaggatat ctggagccaa aacctgcgaa
     2341 agaagagaaa gttctgacgc aatcccaagc tctggacctt ttaagaaaac gcggatatac
     2401 cattatagat agtgcttaaa ccaaagttac gttgattctt cctaaataga ctcgttgtct
     2461 tccatttcaa aaattttgaa aacggtaggc ttatttagtg taccgttctt caggtatctt
     2521 caaatagatt tattttcaaa cttatttcct ttcttctttt actctcctat agcttagcat
     2581 ttaagtgggc actcgtgtca ccttgctaga tagctatgaa tgccgtaata agcctcctag
     2641 tatataggca gactgccaat tataactgat tcaatccaca ttatgataaa atccaccttt
     2701 tcgtttcata tgtgttgaaa tatcacatat gtcaaaagtg cacgagtctt ttcattcagt
     2761 gctttgtaaa tactcaaaat tattcctttt cactttaaaa aaaagataat tagtacaaaa
     2821 tcagaaatga ggaaagtatt aggatggact agaaaggagt tgttgaattt gttgcaacag
     2881 attggtcatt tatcaattaa aaattaggtg aagttttatt catcactgca gctgctgttt
     2941 cccagagaat aaaacgtcta gaagaaactg gtcagttact gtattatcaa gcagcaattg
     3001 actacgaaca agcttgatta ctaaccaaag cgtttatcca cctgttgatt aaatcaaatc
     3061 aaaaatcagg tttttatacg ttcataaaag caatctccag tgtactcgaa tgtgactgta
     3121 tcataggtcc ttattcgatg ttaatcaaaa tggggtttcc ttctaccgaa tcattggaca
     3181 gttttatcaa tgatttatca aagtttggac gaaccaacac ccaaatcgtt ttctcgactc
     3241 ttgtttcctt aatggtttat cagttctgga atgtttatca tgcgctttta ttaaaataga
     3301 aaagagcttt tttcatgcga caaagcagtg atcatttatt ggcacatgca atctttgtgg
     3361 atccattttt tgaaaatacg atatttagtt ttgacccaaa aatctttaaa tacgtatttg
     3421 ataatttatc ttaacaacca gataatttta ttcagcaccg tattatctat attgaattct
     3481 cggaatcaag ataaattttt ttccgttttt gtttcttttt taatcttctt cttgttatta
     3541 atagtgatta ggatgatatt cctatattga tctttgataa ttagatataa tttttgaata
     3601 acgttccctg ctattcgagc tagaaataca agtacgccat gacctagtta gcgacaacta
     3661 cttagagatt tcttttgttc tgggacaaca aaatgccatg acaatagcag taaataatga
     3721 tacttccgta aggctcgccg taaactgggg agaggtgaaa ttcctatgtg cttacccaaa
     3781 gcggttcaaa aattttaaac tattatttta ggagatgatt ttataaaatc gaaagcctac
     3841 ttgaataaaa aacaagcctg tgaatttcta ggaattgata ataatacttt agataagtgg
     3901 attcgtgtat ataattttcc agtaataaaa attgatggcg tagttcgctt tgacgtagag
     3961 gatatcaaag atttcatgaa tacattcaaa aaaaattgaa attttcttct cgtcacatat
     4021 actataaata gcgaaatgta ctgccttact ttttaaagga gtgaggaagt ttgcctacaa
     4081 agctttcaaa tgggaaatat aaaactaatc tgaggtatcc aaaaaaattc agagaaatca
     4141 ctggaattgc atcagagaag tatcaaaaag tatttccaac taggcaatta gctattaagg
     4201 ccgaaaggga aatgaaaaag aagattgaaa cagttttaag agaggagaat gcaaactcac
     4261 tagaattgaa aggaaaaata aaatttaaag atttttatga atcaaaatgg cttcctagat
     4321 atgaactagg tcaaactact aggtcaaaca gggctccaag taatgtaaca attagtaata
     4381 ctaaggatat attccgattg cacatccttc caatgtttgg tgaatatgca atgaattatc
     4441 tgaattcaaa tactgagata atatcagatg aattaacaaa gaaatcgaaa gaatacgcaa
     4501 atataaaaat tatcaaaggc tatgttcgct ctatgtttga tattgccgaa atactatgtt
     4561 atatcgaatt caaccgaact accaaaatta ttcaatgtat taccgcgcct aagaaaatgg
     4621 ctttagaaga aaaaaggata agggaaggga atcaagcatt atcatcagaa gagctgactg
     4681 attggataga agcagtaaac aatgacttaa ataatcactt gcttacactt cacgattaca
     4741 cacttttcat gcttacctta tatctaggtg atagaaaaag tgagacttat gctctgcaat
     4801 ggaaatatat tgattttaat aaaaatacgg ttcgtctcaa atatgcttta gataaatatc
     4861 aaagaaagac acatacaaaa ggttggaaag acaccgttat tcaggcacca gaagttgtga
     4921 tgacattatt aagagaatgg aaatcagctc aggctgaaca attgttgaaa ctaaaaatta
     4981 tacaaacacc tgaccagtac ctctttacct attccaaacc ttctggagaa gtaaattgtc
     5041 ctgttcacgc tgactactta aactatcgaa tcaatgcaat aaaaaaaagg catcctgaat
     5101 tggctcaatt atctccacac aaacttcgtc atacctatgc aactatagct agggaaggtg
     5161 gagcaaatat gaatcaaatc tctaatgctt taacgcactc ggatatttct actacaaaaa
     5221 tatacgtgaa cacacctgac gtagttgata aatctgtttt tgaagcattt caaaaaggat
     5281 tgaataagtg tgattaacat gtgttctcaa atgtgttctt attataaaaa aagacacttt
     5341 ccaaataatt gaaaagtgtc ctgaaatatt gatataaaaa gaatattaac gttttgagaa
     5401 ttgtgaagct ttacgagctt ttttaagacc tggtttttta cgttcgacca tacgtgcgtc
     5461 acgagtaagt aagccagcac gtttaagagc taaacggaag tcaggatcaa cttctagcaa
     5521 tgcacgagcg ataccatgac ggatagctcc tgattgtcca gcgtatcctc cgccgtttac
     5581 gttaacaaat acgtcatatg cgccttttgt ttcagtaaca gcgaaaggtt gattgattac
     5641 ttcacgcaag tcagcatgtg gaatgtattc ttcaacatct tttttattta cagtaatttt
     5701 gccagttcct ggtactagac gtacgcgagc aactgcgttt ttacgacggc ctgtgccgat
     5761 atattgaact tgtgccaatg aaattccctc ctattagatt aagtttgtga tgtctaaaac
     5821 ttctggttgt tgtgctgcat gtggatgttc tgctccaccg tatacgttca attttgtaaa
     5881 ttgtttgcgg cctaaagtgt ttttaggaag catacctttt acagaagttt cgatcaaacg
     5941 acgagaattt ttagcacgca attcgccagc agtgattgat ttcaatcctc ctgggtagtt
     6001 gctgtgacgg taataaattt tgtcagtcgc ttttttacct gctaatttca ctttgtctgc
     6061 attgatgacg atgacgaaat cgccagtatc caaatgaggt gtgaaagttg gtttattttt
     6121 accacgtagt acagatgcaa caactgttga aagacgtccc aatggaacat cagttgcgtc
     6181 tactacatac catttacgtt ctacttcgcc tggtttggcc atatatgttg tacgcacgtt
     6241 attttcctcc aatttaagtt ctgatgtttg acatacactt cgagtttccg gggctctttg
     6301 tggggcaaac aataccattt aacatattac ccgctaatct agtcaatgtc aacgtttttc
     6361 tgcttgattg aaaaatttat atgaaacttt acttcttttc tttcttttat ataaaagggt
     6421 cgtttcctgt caaaatgaaa aacaaaaaga ggctgtgaca aaagtcttgt cacaacccct
     6481 tattttgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatccataa
     6541 aacataagaa gctgtttttt aaaattgttt cttgtgactc ggagctgaac ggcaggct
//
LOCUS       DANKHL010000155         6533 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000155 DANKHL010000000
VERSION     DANKHL010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.164"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            158..1789
                     /locus_tag="PW101_002211"
     CDS             158..1789
                     /locus_tag="PW101_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDL2514600.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1792..2871
                     /gene="uxuA"
                     /locus_tag="PW101_002212"
     CDS             1792..2871
                     /gene="uxuA"
                     /locus_tag="PW101_002212"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2514601.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPACEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2945..4372
                     /gene="uxaC"
                     /locus_tag="PW101_002213"
     CDS             2945..4372
                     /gene="uxaC"
                     /locus_tag="PW101_002213"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL2514602.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            4456..5103
                     /locus_tag="PW101_002214"
     CDS             4456..5103
                     /locus_tag="PW101_002214"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HDL2514603.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            5223..>6533
                     /locus_tag="PW101_002215"
     CDS             5223..>6533
                     /locus_tag="PW101_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2514604.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFF"
BASE COUNT         2214 a          872 c         1351 g         2096 t
ORIGIN      
        1 aaagaaggag tattttattt ttctgaataa aaaatacaat cattctgata tggtagaaaa
       61 aagcaagttt attccctttt tatattgaca acggttacaa tgggttttaa aatataaaat
      121 gtatcaaaac taacatatca gtttgaggag gttaattatg gtttcgatta aaaattggca
      181 aaattctatc gaagaattca atgaaatgca gattctactg ccgtgttttg atgtcaaaga
      241 agttaagcaa gaaggccagc aacatccagt atggcttcat tttggtggag gaaatcttta
      301 tcgaggattt catgcagaaa ttgctcaatg cttagcagat caaaatgaat tagaaacagg
      361 agtagttgtg tgtgaaacat ttgatgaaga agtagtggaa aaagcttatc atgactataa
      421 caatgacatt ttggaaattg ttatgagtgg agatggtgaa ctcaaaaaaa gacttttggc
      481 agtaacggct gatagtttat attgcaatcc gaagaatata gcggactatc aaagagtggt
      541 taaatatttt gaagatccta aattacagtt tacaacgttt actattactg aaaaaggtta
      601 cagtttaaaa gattctaaag ggcaattctt ttcggtagtc aaagctgata tcaaatctgg
      661 acctagcaat cctcaacata cgatgagtat tgtaacatcc ttgttatata gcagatttaa
      721 tgctggagag ttgccgattg cgatggtttc tactgataat ttctcacaga atggtcaacg
      781 ttttcaagat agtattttta cgattgcaga agcttggcaa aaagaaggat atgtttctga
      841 aaaatttatt gcttatttaa aggataaaga aaaagtgact tttccttgga gtatgattga
      901 tcgaattacg ccaaatcctt cagagaatgt aaaggaagat ctattgatga tggggctcga
      961 ggatttagat atcattcaca caaaaaaaca taccaatatt gctgctttca ctaatactga
     1021 ggtagttcat tatttagtta ttgaagatag ttttccaaat ggccggccta aattggaaaa
     1081 ggctggtgtg attatgacag atagagagac ggttgataaa gcagacatca tgaaggttac
     1141 gacaagcttg aatccacttc atactgcttt ggcagtaaca ggttgtctgc taggatatca
     1201 atcaatttca gcagagatgc aggatgatga tttggttggt ttaatcaaag gaataggtta
     1261 tttagaaggt ttaccagtag ttgaaaatcc aggcattatt gatccaaaac aatttattga
     1321 tgaagtaatt aataaacgtt tgcctaatcc ctatattcca gatacaccac aacgaattgc
     1381 aacagatact tctcaaaaag tagcgattcg ctatggtgaa acaattaaaa agtatgtcga
     1441 aaaagacgga aatgcagaaa atctaacatt tattcctatc gctattgctg cttggttacg
     1501 ttatttactg gcagtcgatg atcaagggga aaattttgtg ccaagtcctg atccgttatt
     1561 ggtagagttg caggataaat tatcagtatt gaaattaggt gaacaagatc cttcaaagat
     1621 tcattgtgcg gtacaggaaa ttttagaaaa taaagaaatc tttggtttag atttatatca
     1681 agttggggta ggaaagaaaa ctgaaaaaat cttttgcgaa atgctagtag gtcctcaagc
     1741 agttcgtaaa acaatcaata aatatataac agaagttgga ggaaagtaac tatggaaatg
     1801 acttttagat ggtatggggc aaatgaagaa aaaatctcac tacaagaaat tcgtcagatc
     1861 ccagcaatga aaggaattgt tggaacatta atggatatac cagccggaga ggtttggccg
     1921 agagaacgaa ttaaatcttt aaaagatgaa gtcgaagcca atggacttac cttgaaagtt
     1981 attgagagtg ttaatgttca tgatgatatc aaaattggtt taccaacacg agatcaatac
     2041 attgagaatt ataaacagac gattcgcaat ttggcagaat ttgggattga ggttatctgt
     2101 tataatttta tgccagtatt tgattgggta aaatcggatt tggattatcg tttagatgat
     2161 gggtcttcta cgttagccta tatttcaaaa aatatcccta ctgatccgga ggaaattatt
     2221 agagaagtag aaaattctac taataatttt gaattgccgg gttgggaacc tcatcggtta
     2281 tcacaaatta agcaattatt agaagcctat aaagaagttg atgaagagaa attacgagag
     2341 aactttgcat attttctcaa agcaattatc ccggcatgtg aagaagttgg gattaaaatg
     2401 gcagttcacc cagatgatcc accatattcc attttcggat tacctcgtat tgttaaaaat
     2461 cgtgacgatt tagattggtt gtgtaatgta gtggattctc cttctaatgg tattacatta
     2521 tgtactggaa gtattgcaga acgaccagat aatgatgtct atgaaattct atctgaattt
     2581 tgtcaacggg atcggattcc ttttgcacat gttcgtaata tcaaatattt aagtgacaat
     2641 ggactcgatt tttacgaagc gccgcataaa tcagaatatg gttctctaga tatgtataag
     2701 atattaaaaa caatgtacga taatggattt aatggctata ttcgtccaga tcatggtcgg
     2761 atgatttggg gagaaaccgg tagacctggt tatggattat atgaccgagc tctaggtgca
     2821 gtttatttaa atggtatttg ggaagcgctt gaaaaggggg cgagtaaata attatataag
     2881 ggagtttatt tagaggaaaa attttctctt ctaaaaacta acggaaggaa aggagtgttt
     2941 aaatatgttt ttagataaga actttttatt agagaatgaa atagctaaaa gactttttca
     3001 taaatttgca aaacaccaac caattatcga ctatcattgt catttagacc cagcatattt
     3061 gctagaaaat aagcaataca agaatttgac tcaggtatgg ttaaatgata aaggagctgg
     3121 agatcattat aaatggcgat tgatgcgagc taatggtata gaagaagagt tgatcagtgg
     3181 tccaggtgat gattatgaaa aatttttagc ttttgtaaaa acagtggaga aggcgccagg
     3241 taatcctatt tttgaatggt ctcatttgga attacaacgt tactttgata ttgatttgac
     3301 aatttctgag aagaatgcac cagaaatttg gcgacgtgct aacgaaaaaa ttcaaacgga
     3361 ttcctatcgc ccgaaagaat tgttggagat gatggatgtg aaagccatct gtacaacgga
     3421 tgatcctaca gatgacttgt ggtatcatac tgagctttct aaacaggata attctttcaa
     3481 ggtattacct actttccgtc cagataaggt ctggagtatc aaatcatcag gcttttcgaa
     3541 ctatatttta aaacttagtc aaacagcggc tataccaatc aaagacttta atgggttgaa
     3601 gcaagcattg agccaacgag tggatcattt tcatcaaatt ggctgtcgat tagcagatca
     3661 cggtttgaat acttattttt atgaaatagc agatgaaaaa atactcgaca atctgttaat
     3721 aaaagcttta gcaggaaaaa cggatttcca tttatcagaa attgcacaat ttgttacagg
     3781 tattcaatta catttgatgt ctgagtatac aagatatggt tggacgatgc aaatgcattt
     3841 aaatgcttta agaaataatt caagtatatt aaaggaacaa attggagtag atgtcggtgg
     3901 tgattcgatg ggggatcaag ctagtttgac acaaaatgtt gtagcattgt tcgataccgc
     3961 tgaaaagagt gggtgcttac caaaaacaat tttatattca ttaaatccaa ctgattggct
     4021 tccactagca acagcaatgc aaagcttcca agggggtatg attcaaaaaa tccaattggg
     4081 atgtgcttgg tggttcaatg atacagctga aggaatgcgt caacagttga ctacgatggc
     4141 acaacaaagt ttgttgtcga attttacagg tatgttgact gattctcgaa gttttctaag
     4201 ctatcctcgc catgagtatt ttcgtcgagt attatgtcaa ttactaagcg agtgggttaa
     4261 agcaggccga attccagatg atgaaatgta tcttagtcag atagtggaga atatttgtta
     4321 tcgtaatgcg tataattatt ttaatttttt tgaaatcgga agtagaaaat aatacttgaa
     4381 ataaaagctt aaatttattc tttgtaagtt agtacagcta gacaaagtat gtatccataa
     4441 aggaaggaga tagatatgaa aaaagtagaa attttaagta agttagaagc ggctggagtc
     4501 attgcggttg tacgtgggaa aaataaggat gaggccatta aagcaataaa ttcaattatt
     4561 gcgggtggaa ttaaagggat tgaactaact tttacagttc ctaatgctga agaaataatc
     4621 aaagaaattg ctaaaaaata tgctgatcaa aaagatgtaa taatcggagc cggtacggtt
     4681 ttagatgcgt ttactgctcg tatcgcaatt atggctgggg cagaatatat cgttagtcca
     4741 tcttttgatc gacaaacagc tgagatttgt aatttatatc aaattcctta tttaccaggt
     4801 tgtatgacaa ttactgagat aaaggaggca cttactgctg gcgtagatat cattaaacta
     4861 ttcccaggtt ctgcttttgg tccatccatt gtaaaagcct atcaagctcc attaccacac
     4921 gtaaatataa tgcctactgg cggagtaagt atagagaact tagaagattg gtttgaagta
     4981 ggagttgttg cagttggtgt aggtgggaat ttattggctt ctgttgaaaa aggtgatttt
     5041 gaagaagtaa gtcgagtagc tagtaaatat atagataagt tttatgagat aaagaattat
     5101 taggagaaaa aacaaataat actctgaaat gtaaaaaata aatactgact ctttacgctt
     5161 cagagtatag tacgtacttt catcagaatg aaaacgctta acaacagaag ggagagaatt
     5221 gtatggaaag aaaatttggt ataagagaca aaataggata tatgtttgga gattttggaa
     5281 acgattttac tttcattttt gctagttctt ttttaatgat cttttataca aaagtattgg
     5341 ggataagcgg agctgccgtt ggaacattat ttttagtagc aagatttatc gatgcagtta
     5401 ctgatgtaac tatggggcgt attgttgata gatcgaaagc aactaagcag ggtaagttta
     5461 aaccgtggat tttaagaatg agtggtccag tcgctttagc aagtttttta atgtatcaaa
     5521 cttctatgat ttcagcttct atgagcgtta aggtagtata catgtatgtt acgtatatat
     5581 tatgggggag tattttttac acatctataa atattcctta tggatcaatg gcatcagcta
     5641 ttacttctat tacagaagaa agaacaatgc tatcgacatt ccgaaatgtt ggagctactt
     5701 tagcaagttt ggtcattggt gtattaacac cattatttat ttatacaaga gatgattcag
     5761 gcgctcaaat agttcgtggt ggatcaactt ttacaattgt tgcgggaata ttttcgatat
     5821 gtgcacttat ttgttattta atatgttata aactaactac tgaacgagta agaattgacc
     5881 aatctcaata caaagtttca ttgttaaaat ctttaaaagg gttagttaaa aatagggcat
     5941 tactttctat aattggtgcg gctattttct tactccttgc tcaattatta attatgagta
     6001 tgaataatta tgtatttcct gattactata atgatgcaca gggaatagtt ttaatgaatt
     6061 ttatcaatcc tattttggtt ttaatagttg tttcgccttt aactttgttt ttaagtaaaa
     6121 aatatggaaa aaaagaactg gcatcagtag gaatgttctt ttcagcaata gtatatttga
     6181 tcttattttt gataaaagct agtaatatgt atgtattctt aattcttagt tcggttggct
     6241 atatgggttt gggtgtgttt aatactgtta tttgggcaaa tattactgat gttattgatg
     6301 atcaagaagt aaagtctgat caaagagaag atgggaccgt atatgcggta tattcttttg
     6361 ctagaaaaat aggtcaagct ctagctggag gcgtcggagg ttggactctt tctattatag
     6421 gctatgactc tttagcaaat gtacaaacgg gagatgtttt agaaaagtta tataacgctt
     6481 caacattaat tccatcaatt tgttttctga ttgtaggatt aattttattt ttc
//
LOCUS       DANKHL010000156         6505 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000156 DANKHL010000000
VERSION     DANKHL010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.165"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(51..632)
                     /locus_tag="PW101_002216"
     CDS             complement(51..632)
                     /locus_tag="PW101_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDL2514605.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(638..1897)
                     /locus_tag="PW101_002217"
     CDS             complement(638..1897)
                     /locus_tag="PW101_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL2514606.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(1899..2945)
                     /locus_tag="PW101_002218"
     CDS             complement(1899..2945)
                     /locus_tag="PW101_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514607.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(3180..3455)
                     /locus_tag="PW101_002219"
     CDS             complement(3180..3455)
                     /locus_tag="PW101_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDL2514608.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(3698..5008)
                     /gene="der"
                     /locus_tag="PW101_002220"
     CDS             complement(3698..5008)
                     /gene="der"
                     /locus_tag="PW101_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDL2514609.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(5190..6419)
                     /gene="rpsA"
                     /locus_tag="PW101_002221"
     CDS             complement(5190..6419)
                     /gene="rpsA"
                     /locus_tag="PW101_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDL2514610.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
BASE COUNT         1827 a         1403 c         1053 g         2222 t
ORIGIN      
        1 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
       61 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
      121 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
      181 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
      241 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
      301 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
      361 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
      421 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
      481 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
      541 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
      601 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
      661 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
      721 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
      781 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
      841 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
      901 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
      961 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     1021 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     1081 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     1141 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     1201 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     1261 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
     1321 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     1381 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     1441 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     1501 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     1561 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     1621 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     1681 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     1741 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     1801 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     1861 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     1921 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
     1981 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     2041 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     2101 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     2161 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     2221 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     2281 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     2341 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     2401 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
     2461 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
     2521 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     2581 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     2641 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     2701 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     2761 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     2821 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
     2881 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     2941 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     3001 agataagagc aaagctcacc tcataacttg caaataaata aaaattaaat aaaatacatg
     3061 tgttttctat gtatgacata cggattcgga atctctgtct cttatttatt ccttttctgc
     3121 taggaaataa aaagaaaagc acttgtagcc ctagagccac aagcgctttt tcaactgtct
     3181 tatttaacag cgtcttttaa tgctttacct ggtttgaatg caggtacttt gctagctgga
     3241 atttcgattt cttcaccagt ttgtgggtta cgacctttgc gagctgcgcg ttcgcgaact
     3301 tcaaagttac caaaaccgat taattgaact ttttcgccgt tagctaaagc atcttgaata
     3361 gttgaaaata cagcatctac tgctgcagtt gcatcttttt tagttaaatc agtagctgaa
     3421 gcaacttttt cgattaattc tgctttgttt gccatggata gattcacctc ctccacaaga
     3481 gatatgatag aaatatcatt tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa
     3541 atattgatta acttcaatat tctgttatca cgatatcata tgaaccgctt aatagcaagg
     3601 tttttaccaa ttttcacatg ctttttttag tcatagaaac gtaacaaacg aaactgtctt
     3661 tttcttttga aaaaaaaacg tcgatatggt aaaatgccta ttttctccta cgagggataa
     3721 ttcggatgga cgtcccttca aaagtaaatg cttttctgat ttgattttct aagaaacgtt
     3781 cataagaaaa atgcatcaat tcctcttcat tgacaaaaac gacaaatgta ggtggtttga
     3841 cagcgacttg tgtagcataa aaaatcttca ggcgttttcc tttatctgtt ggtgttggat
     3901 tgattgctac agcatccata atgatatcat tcaacacagc cgaagagata cgaagattct
     3961 ggttcatact tacttgttcg atcatttcag gcagacgttc caaccgttgt ttagtcactg
     4021 cagaaacaaa aacgattgga gcgtaatcaa gataccggaa ttcatcacga atctcttgtt
     4081 cgaaatcacg cattgtattg gtttctttct ttacaagatc ccatttattc acgacgataa
     4141 taattccacg accagcttca tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc
     4201 cttcttcagc attcagtacc atcaaaacga tatcagaacg gtcaatggca cgcattgcac
     4261 gcatcacgct gtatttttca gtattttcgt atacttttcc gcgtttacgc ataccagctg
     4321 tatctatcat taagaatttc tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc
     4381 gggtcgttcc ttcaatatcc gaaacaatta cacgttcttc tcctaaaatc gcattgatca
     4441 aagaggattt accaacattt gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct
     4501 cttcttcagt ctcttcagag aaatacttca ctgcttcatc taaaatatca ccaatcccta
     4561 gtccatggct tcctgaaaca ggataaggat cgcctagccc taaggaataa aattcataga
     4621 tatcgtttct catttctgga ttatctactt tattgactgc taaaatcact ggtttattac
     4681 ttctgtataa aattttcgca accatctcat ctgcgtctgt cacgccttcg cgtccgctga
     4741 ctacacaaat gattacatct gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat
     4801 ccataaaagg ttcatcgcca agatcaattc cgcctgtgtc gatcacgctg aattctcgac
     4861 ctaaccattc accttttgca taaatacgat cacgagttac tccaggagta tcttcgacaa
     4921 tcgagatacg ctcaccagcg atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc
     4981 caacgattgc aattgttgga tttgccatat tatttcctcc tcacaagttt ccaatctcac
     5041 ttagtttaac gaatgcattg ccaactttca agcatagatt gtcaattaaa cgaagtattt
     5101 ttattcctta ttgaaatact aatcgctttt caaaagaaaa actgagacaa aagcagctgc
     5161 tttcatctca gtcacagata caaaatcaat tatttttcag aatcatcggt tgctttttct
     5221 tctacttctt ctttcaacgc atcacctaaa atgtctccca tagtaaatcc agtattttct
     5281 tctggtaatt cataagactc aacatttttt ggttcttctt ctgaagctgg tttttcttct
     5341 aaagctttga tactcaatgc aatacgatgt tcttctggat ggatctctaa tactttcact
     5401 ttcacttcgt caccttcatg aagcacttca tgtggtgtcg caatatgttt atgtgaaatt
     5461 tgagagatat ggactagccc ttctactcca gggaacactt caacaaaggc accgaagctt
     5521 gtcaaacgtt taactgttcc gtctaatact gtgccaactg gagcttttgt atcgatttct
     5581 gaccaaggtc caggctgtgt gtctttgatt gacaaggaaa cacgttcttg ttcaggattg
     5641 atagacaaca ctttgacatt tacatcatca ttgacagcca atacatcact tggttttgct
     5701 acatgactat gtgaaatctc tgagacatgg actagaccgt caattccgcc taggtcgaca
     5761 aatgcgccaa aatcagtaag acgtgcaaca cgtccactga gaacatctcc ctcatggata
     5821 gttgaaagaa tttctttttt cttttcttct ctttcgtttt ccactaccgc tttatgcgat
     5881 aagatcaaac gattttctga tggttcgatt tcgatgattt taaagctcaa tgttttgcct
     5941 ttatagtctg aaaagtctgc aacaaaatga tcttccacca ttgatgcagg gacaaaacca
     6001 cgtacaccga cgtcaacgac taaaccgcct ttgacaacat ttgtaacagg tgcttcgatg
     6061 accttgccct cttggaaatc tctttcgata tcttcccaaa cttttttagc atctaaacgg
     6121 cgttttgaaa gtagatagct gccgttttct ttatctttgc cgatcgtact gataacgact
     6181 aaatctaata catcaccgac ttttactact tcgctgatat cttctaccgg caaagttgat
     6241 aattcttttg ccggaacgac tccttcaact ccagcccctt cgattccgac aatcacttgt
     6301 ttgtcttcaa tgactaaaac ttcgccctta acaacatcgc caacttttac ttcttgtacg
     6361 cttttcattg cgtcttccat tgactggttg ttctcttgat tttgatcaaa ttctgtcata
     6421 acacttgtcc tcctaaccaa cattctgccg taagtacggt actgttttta gtttaattga
     6481 ttattcaaaa aaaataaagc gattt
//
LOCUS       DANKHL010000157         6504 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000157 DANKHL010000000
VERSION     DANKHL010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.166"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            47..784
                     /locus_tag="PW101_002222"
     CDS             47..784
                     /locus_tag="PW101_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2514611.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            798..1613
                     /locus_tag="PW101_002223"
     CDS             798..1613
                     /locus_tag="PW101_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL2514612.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1626..2273
                     /locus_tag="PW101_002224"
     CDS             1626..2273
                     /locus_tag="PW101_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2514613.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2289..2945
                     /locus_tag="PW101_002225"
     CDS             2289..2945
                     /locus_tag="PW101_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2514614.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3107..3928
                     /gene="racE"
                     /locus_tag="PW101_002226"
     CDS             3107..3928
                     /gene="racE"
                     /locus_tag="PW101_002226"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDL2514615.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3931..5286
                     /gene="rph"
                     /locus_tag="PW101_002227"
     CDS             3931..5286
                     /gene="rph"
                     /locus_tag="PW101_002227"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDL2514616.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5287..5805
                     /locus_tag="PW101_002228"
     CDS             5287..5805
                     /locus_tag="PW101_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2514617.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5912..6412
                     /locus_tag="PW101_002229"
     CDS             5912..6412
                     /locus_tag="PW101_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2514618.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         2138 a         1094 c         1437 g         1835 t
ORIGIN      
        1 catctttaaa agaaatctta ctaaataata atgggagtgt aattctatgg caatgattaa
       61 atttgatcac gtggataaat attatggcaa gttccacgca ttaaaaaata tcaatctcga
      121 attcgagaaa ggagaagttg tcgttgtcat tggtccttct ggttcaggaa agagcacgat
      181 gcttcgctgt atcaatggat tagagacgat ctcttcaggt aagctgctga ttaatgatac
      241 tgacttgcat gataaaaaga caaaactgac agaagtccga aaaaacatcg gcatggtttt
      301 ccaacatttc aatttgtacc caaataaaac tgttctggaa aacatcaaat tagcaccaat
      361 caaagtgttg aagcaagacc aggcaactgc agtcaaaaac gcggagaaat ttttgaaaac
      421 tgtcaatatg ttagataaga aagattctta tccttccatg ttatccgggg gacaacagca
      481 acgggtggca atcgccagag gtttagcgat gaatcctgag atgctgctgt ttgatgaacc
      541 tacttctgct ttggatccgg aaatgattgg tgatgtatta gacgttatga aaaaattggc
      601 tcatgacggt atgtcaatga ttgtcgtgac acatgaaatg ggctttgcca aagaagtggc
      661 ggatcgtgtt atcttcatgg ctgatggaga agtattggaa gacagcagaa atgtgagaga
      721 ctttttcgaa aatccaaatg aagtccgagc acagcaattt atcagtaaag ttatcaatca
      781 ttgataggag gaaaaagatg cgcaggttaa aaaaaatacg cctgattttc cttttaggca
      841 ttgcttttct tgcactagcc ggatgtaaag gaaatgggtt ggcaaatgaa gatattttag
      901 agcgaagcaa atctaccaat gaaatcatct ggggtgtcaa gtatgatact cgattatttg
      961 gaatgatgga tattgagagc agaactgtac aaggatttga tgtagatatt gcaaaagcaa
     1021 tcactaaaaa gatccttggt gacaatggaa aaacagaatt tgttgaagtc acttctaaaa
     1081 ctcggattcc attattgaaa aacggaaata tcgatgcaat tattgcaacg atgacgatca
     1141 cagatgaacg aaaaaaacag gttgactttt cagatgtcta tttcgatgct ggccaagctt
     1201 tgttggtaaa gaaaggaagc caaatcaaaa gtgtagatga tttgaatgct tctacaacgg
     1261 ttctagcagt caaaggttct acatcggctg cgaatatccg acagcatgca ccagatgcaa
     1321 aaattttaga attagaaaac tatgcagaag catttactgc tttacaatct ggtcagggag
     1381 acgcgatgac tacggataat gcgattttgt tagggattgc agatgaaaat ccagagtatg
     1441 agttagttgg tggaacgttt actaatgagc cttacgggat tgcgatcaac aaagggcaag
     1501 aaaacttctt gaaagctgtt aaccaagcgc tagaggaaat gcatgcagat ggcacatacg
     1561 ataaaatcta tcaaaaatgg tttccaaatg aaactgaagg aaaagtagag taagggggag
     1621 aggcaatgag cgaattgata caaacttatg gaccaatctt tgtcgacggg ttcaagatca
     1681 cattgttttc gagtgtctta gcacttgtgt ttagtttgat tatcggaacg ttaatggcaa
     1741 tcttccaatt atcaaagaat aaagtagtga gcgccttagc aaaggcatat gtagaatttt
     1801 ttagaaacat tccattatta atcatcgtga tgttttttta tgttgtcgta ccgatttatt
     1861 ggtttaatct agatggattc caagcaggta cgattggatt aacgatctat acatctgctt
     1921 ttattgctga aacagtacgt tcagggattc aaaccgtacc aaaaggtcaa acagaagcag
     1981 gactatcttc tggttttacc tattcagaaa tcatgcgtta tattgtttta ccgcaagcat
     2041 tcaaaatcgt tatcccgcca ttaggcaacc aatttatcaa cctcgtaaaa aactcatcta
     2101 ttttagcaat cgtagctggt ttagatttga tgtatcaagg agacttgatc gctagtgaaa
     2161 cattcaatac cttcgatacg tatatcatcg ttgggttatt ttacttagtt atcacgctac
     2221 cgttatctta cttaatggtt tatcttgaga aaaaatgggc ggttcgttca tagaaggagg
     2281 ggaaatacat ggattttagc ggcgcttttt catggatgaa tatccgtttt ttattggaag
     2341 gattaaaggt tactgttgaa gtttcattgt tttcgatcat ttttagtttc ttgatcggtg
     2401 gtctgacagg tgtgattcgt tttgcgaata tccctttctt ttccaaaatc cttggtgtag
     2461 tgattgatat tatacggaat cttccattat tattgatcat tttcttcact tactttgcct
     2521 tacctcaaat cggcattcag atgaacatct tctggtccgc tgtcgcagca ttaacgattt
     2581 ttgagtcagc tatgttgtcg gaaattttcc gagctggact caatgctgtt ccaaaaggac
     2641 agatggaagc aggattatca acgggactga cttatgtaga atcgatgcga acgatcgtgt
     2701 taccgcaagc atttaaatca atgatcccgg caattgtgag ccaattgatt tcactgatca
     2761 aagatacttc attagcagta atcatttctt tacctgaact ttctcatcag gcacgtatta
     2821 tttatggaca gaacaccaat tatgtgcttc cgatgttcat catcatgaca cttatgtact
     2881 tcgtggtatg ttacgcgctt tctctgcttt ccggttactt ggaaaagaga cggtacagtt
     2941 attaatagat gattctttta gcggttgtgg caaactaaag tcacaaccgc ttttcctctt
     3001 tccattcgaa aaaaagagat ttcgattgtt ttaagcaagt aagagcactt gaaaattcac
     3061 gctaaaaagt acacaatagg gaggatagaa tttattgaaa aaaaggatga tacgattgac
     3121 agataatcgc cctatcggat ttattgattc aggtgtcggc ggcttgactg tagtaaaaga
     3181 agccctgaaa caattaccga atgaaaatat tttatttgta ggagacacag cacgctgccc
     3241 atatggccct agacccgcgg aacaggtaat ccagtatact tgggaaatga cggattatct
     3301 ggtggagcaa ggaatcaaga tgctggtgat cgcctgcaat accgcaactg cggtggcttt
     3361 agaagaaatc aaagctgctc tttctattcc agtcatcggt gtgatccttc ccggtactag
     3421 agcggcagta aaaaaaacac aaaataaaca agttggcatt atcggtacga ttggtacggt
     3481 aaaaagtcaa gcttatgaaa aagcactgaa agagaaagta ccagaattga ctgtgacaag
     3541 tcttgcttgt cccaaatttg tttcagttgt cgaaagtaat gaataccatt catcggtggc
     3601 gaaaaaaatt gtggcagaaa cattagctcc tttaaccact aaaaaaatcg atacattgat
     3661 tttgggatgc acccattatc cattattacg ccccatcatt caaaatgtaa tgggagaaaa
     3721 tgttcaactg atcgattctg gagcagaaac agtaggtgaa gtatctatgc tgttagatta
     3781 tttcaatttg agcaattcac cgcaaaatgg tcggacatta tgccagtttt atacaactgg
     3841 ctctgccaaa cttttcgagg aaatagctga agactggctt ggaatcggac acttaaatgt
     3901 agaacatatc gaattgggag gaaaataagg atgcgtcatg acgggagaaa agcccaagag
     3961 cttcgcaaac tggagcttca aacaaatgtt ttcaaatatc cagaaggatc ggtagtgatc
     4021 cgttttggtg atacaacagt tatttgttct gcaacaatag aaaattctgt tccccctttt
     4081 ttgagagaaa caggaacagg atgggtgaca gcagaatata gtatgctgcc tcgagcaacg
     4141 agtacgagaa atcgtagaga aagcagcaaa gggaaacttt ctggtagaac aatggaaatc
     4201 cagcgtctta ttggtcgttc cttgcgtgca gtagtagact tggaaaaatt aggagaacgt
     4261 agtatcatcg tagactgcga tgtgatccaa gcggatggcg gtactagaac agcaagtatc
     4321 actggggcat ttgtggcatt gaaattagcc attgaaaaac ttttaagaga aaaagaattg
     4381 tcagaagatc caataaaaga acatcttgct gcagttagcg taggaatctt accagatggg
     4441 acttgcgtca cagatttgga ttaccaagaa gattccgcag cacttgtaga catgaatctt
     4501 gtaatgacag aatctggaaa attcgtggag attcaaggaa ctggagaaga agcaacgttt
     4561 gatggtgaac agttgaatga aatgctcttt tttggaaaaa acgcgattga agatttgatc
     4621 aaagagcaaa aacatgcttt gctaacagag tttgctcaaa atgatgagcg gatagaagaa
     4681 acaaaaacaa tcatcattgc gacaagaaat ccaggaaaag cagaagagtt tcgaaatatg
     4741 ttcaaagaag cgggatatca tgtaaaaaca ttacttgact atcctgaatt gccagatgtg
     4801 gaagaaactg gcagcacatt tgaagaaaat gcccgattaa aagcagaaac gattgcacaa
     4861 ttgcttgatc agccagtctt agcagatgat tcaggtttga aagtggatgc tttaggcggt
     4921 atgccaggaa tctattctgc acgttttgca ggggaacaaa aaagtgatgc ggggaataac
     4981 gccaaattat tatatgaatt gacagatgtg ccggatgaaa aaagaacagc acaattccat
     5041 tgtacgttag tattcgcggc acccaaaaaa gacagccttg tagtagaagc ggaatggccc
     5101 ggtcgagtag cccgtatacc atctggcgaa aatggctttg ggtatgatcc tttatttatc
     5161 ccagaaggta agaaacaaac agcagcagaa ttatcgagcg aagaaaaaaa caagatcagt
     5221 catcgggctc aagcaatgaa aaaattgagt gccgagtgga aacaatggct ggaaggagag
     5281 agatagatgc ggtatttagt agtaagtgat aatcatggag acagagatat cctcgttgac
     5341 ttagtagatc gttatcgttc tgaagtggat atctttttcc attgtggtga ttcggaatta
     5401 gaagcttctg acccattatg ggatacattc caagtggttc aaggtaattg tgattatgga
     5461 ccaggattcg aacagaaaaa agtgatccaa actggtcagg atacagtatt catgactcac
     5521 ggtcatttat cgaatgtccg atttggtttg acacagttag caatcgaagc tcgagctgca
     5581 actgccgata tggtgttttt tggtcatacc catcaaatag gctgtgagat ggatcagaaa
     5641 atcctgtttt tgaatcctgg tagcatttct cagcctagag gcccgatcca aatcccatca
     5701 tatgctctta tcgaaagttt agatgaccaa atttctgttc aatactataa ccgtgcccat
     5761 caggcagttg aagagttggc cttttctttt tcgagagagc gctgaataaa aaaactctaa
     5821 tttttaataa aaataagctt tattttatga attttgcatc cggtttcggt aaaatagtag
     5881 tgttgaatga aatagaaatg gaggccatca gatgattgga cctatagtaa gagaaatgct
     5941 gctggaaaat caggaaacgt ttttgattcc tgcagaaaat gtggcaaatg ttatgtatca
     6001 tcatccattg tcccatggtt tacttgtgtt atcgaaggtc ggttattcca aaattcctgt
     6061 attaggaaaa gatgatcact ttgtaggact agtaagttta tcagatgtgg tcaataaaat
     6121 gattgacttg caaacaatca gcatggaccc gttagaagga ctaacggttg ctgatgtgac
     6181 agaaacagat gttccgacaa ttgatgaaaa ttgggaacta gaagaagtac tccatttgtt
     6241 agtcgattcg ccttttttac cggttgtcac agaagatcgg attttcaaag gaatcattac
     6301 aagaaaagag ctgcttaaag ccgtaaactt catggtgcat gaattagaac gtcgtaatat
     6361 attgtctccg aaaatcgaag aattaaaaga acaaatcgat atcgtgagct aaagaacgag
     6421 aaactaaaag ataatcaaaa aataagtaga aaaaacagcg taccagtttt gactttatct
     6481 tgaaaaagag gctgggacaa aagc
//
LOCUS       DANKHL010000158         6407 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000158 DANKHL010000000
VERSION     DANKHL010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.167"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..625)
                     /locus_tag="PW101_002230"
     CDS             complement(<1..625)
                     /locus_tag="PW101_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514619.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPI"
     gene            complement(725..1234)
                     /locus_tag="PW101_002231"
     CDS             complement(725..1234)
                     /locus_tag="PW101_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDL2514620.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(1435..2304)
                     /locus_tag="PW101_002232"
     CDS             complement(1435..2304)
                     /locus_tag="PW101_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2514621.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(2324..3445)
                     /locus_tag="PW101_002233"
     CDS             complement(2324..3445)
                     /locus_tag="PW101_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDL2514622.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(3447..3842)
                     /locus_tag="PW101_002234"
     CDS             complement(3447..3842)
                     /locus_tag="PW101_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2514623.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(3861..4667)
                     /locus_tag="PW101_002235"
     CDS             complement(3861..4667)
                     /locus_tag="PW101_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2514624.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(4660..5448)
                     /locus_tag="PW101_002236"
     CDS             complement(4660..5448)
                     /locus_tag="PW101_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2514625.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(5462..5938)
                     /locus_tag="PW101_002237"
     CDS             complement(5462..5938)
                     /locus_tag="PW101_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2514626.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(6010..>6407)
                     /locus_tag="PW101_002238"
     CDS             complement(6010..>6407)
                     /locus_tag="PW101_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2514627.1"
                     /translation="NRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         1982 a         1382 c          904 g         2139 t
ORIGIN      
        1 aaattggtac ttttttatta attagttcat cgcccaataa tctctccagt ttcttagcat
       61 ctcctgactc aaccggaatt atccaagccc agtccaaata attttttaaa atctcatcat
      121 attttgcaaa tatttcattg ccatattccg tccttaaaat tatattttca gtgccagaca
      181 agtgatttgt attgagttga taaaaaagat ctccatctaa aaaaatatca ttagaagaag
      241 atttccccac agtaatgacc attccatcag ctctatgtgt tacatacaaa taattgtatg
      301 acctcatttc acctaatgaa tcaattattt cttgcccttt agctctaata tattgatctc
      361 tattacctat ctcactctta tttaacaaaa gttttttcat gtcaaattta atcggctgtt
      421 ttatttttgt aaattcgctt ctactactaa tccaatccga gattaatttt tcaacatgtc
      481 ccagagtaaa ctctgattta ttcgctaaat actttttttg ttgtattaaa gacatctttc
      541 cccgaagctc aaaattgagc ctacatcgat tctctatctc ttcaaaaagc atcttatctt
      601 catcattctt atattcatca ttcataaaac caatccagct cctcgattaa gataatattt
      661 taatctcata caaaaactag ctctcatcaa aacaaccatt gttttgattt ctcatgaaat
      721 ttctttatac ttttctccga ttataaattc ctaacccctt gagaaaatta cgcatatatt
      781 tatctccgca aggcttaaaa tttcgttggc cttcttttct caaaaatgcc ccgatttcgc
      841 cttttgatgg atagatttct gcctcagcaa aaacgtctaa aatatcatca gtggtcatag
      901 acaaggcgat ttttaatttt tttagcaaca cattgttgat atatttgatt tccttgcttt
      961 tttgcggcgt gtctactatc ggcttatctt tttgcggacc acgcgccaag agaatcaggc
     1021 catttaggaa tgcgtcaaaa acttggttgt tgatggtttt ttcatacaca ttattttctg
     1081 cattatcttt gttcttatcc tgtactttgg tcagtaccgc ctgagcttct tctttagtca
     1141 gtggtacacc gcctagctca aaagctttaa tcaaatccgt atctttcaaa tcaatcgcat
     1201 aacgcaatcg caataaccta tcattgttat tcatttgttc ctccgaagta tttagtcttt
     1261 actttcttac gtactttttg tcttgctaag atttttagga tcaatgataa aaaccgcatc
     1321 acttatgata aggcgctggg tatcatgagt gaatgatatt gctgaataca aatagtccga
     1381 ccacattaag ttgatcggac ttcataagca ttttattgac taatactttt ttgcttaaga
     1441 attgattcta acatcttttg ctaaagttaa gcatcctata gttcctgcat tatctgctaa
     1501 cttaggtgtg acaatgtatt cttcgatatt tggcgtatcg acatagccgt taattagttc
     1561 taaaaaacgt ctttgtactt tctttttcag atgttcttgt tgcatgacac caccaccaaa
     1621 aattataata tctggtgaaa gcatcagggt cgtattacag gcacattgtg caatataatc
     1681 cgcctcgatt tcccagaacg gatcttctat agaaagatct tgtcccttgg ttcccgtacg
     1741 catctcgata gcgggtccag atgccaaccc ttcgagacaa tcatgatgaa aaggacaagt
     1801 accagagaaa gtatctcctg aatgtctctt aactaacgta tgccccattt ctggatggct
     1861 aaacccttca acaaaaatcc catcttgtat agctcctcca ccaattccag ttccaacagt
     1921 gtaataaaca acactggata aaccttttcc atgacctgaa acatactccc cgtaacaagc
     1981 agcattcaca tcggttgtcc aggcaattgg aatttcgaaa tgcttattca acgttccaac
     2041 aaaatcaaaa tttttccagg cagtttttgg agtagaggtg atatgtccat aggtctttga
     2101 ttcacgatgg atatcaatcg gtccaaatga ccctactcca atgctttcta attgttcttt
     2161 atagtgatta aagaaatcaa ttaccaaact cattgtttca ttcggtgtag ttgtaggaaa
     2221 actcactctt tctaaaacct ttaaatcatc attgccaatg gcacaaacaa atttagtccc
     2281 acctgcttct attgagccat acatttctta ctccaccgtc cttttatcta ggaattcgtt
     2341 cttccctgtc taatagtttt tcgaaaaact catgtggttc cttttggtac caataggcaa
     2401 cagaagaaat atcgtcttga cgctcataaa gcttgatgtc atcatttcct agggtttgga
     2461 aagtaacacg taaattttct ttgaaactaa tggggtcggg taaatgccag cggtacaacc
     2521 catgcatcgg taaagcatca tcgccaaaac catgaactgg atttggatta ggttttccag
     2581 cactaaaata atcccgggtg tgatctcttt tagattgaaa aggatagccc ataaatagcg
     2641 tattaaaatt ttgagcatat gccctttctc caaagttctt tttatggaaa gcccaagcgc
     2701 cgccaaaata atcttctgct cctgtagatg ttattgtagg gaaatcttca tctccatcta
     2761 tataaaactt aaattctcct tccccccacc agtatctttc caatgcgcac aaagcaaagt
     2821 aggttcctac ataataccca gtgcctttga ttccatcaat aagggtgtaa tcttttcctt
     2881 ttgtcgtgat gggttcacgg ttccatttag catgaaaata catcgcatca tccggtactt
     2941 cagtcaatgc ataattaaat gtataaaaaa tgtgcttaat aaactcagga tgttcattcg
     3001 ttaaagtaat cttagctttt tttcgaaatg gcatctcaaa atagcaattc atccctgatg
     3061 ttggattcac tacgattggt aaggaattca cttcgcatct tgtcccaaac ccattacaaa
     3121 aaaaatctcc taatggactt tctacagaag gagtggattc atcatcccaa taaatacgca
     3181 aaattaaatc acgtaagacg aaactcccag cgattgtttt gtctgtcact gtcatccaaa
     3241 aatgacgtaa aattccaggg ccttccattt ctgctaaagt aattgtctct ccagcattta
     3301 tcggaacaga aggtgaccct tttctcccaa ttcctagatg actcgctgtc attgccgctt
     3361 tccctttttc accattttta ttttctggtg aaatagttct actttcctct cccttgtgga
     3421 gccatatatt atttaaacaa ttcattttat tcgtcctcta tcctttcttc acttttttga
     3481 ttattcatat agatgatttg ttgttgtgcc tccgcaataa ttgctccaat atctaaatta
     3541 tcatttccta actgcaaagc gattgacatt gccaagggaa gattcattcc tgcaattacg
     3601 tgcactctgt cagaaatata ggtaaaaaac ttttgattta cacttcctcc agccatgtca
     3661 gttaaaacaa ttatttcgtc tgtagtatcc actaactctt taatttgttc atctatatca
     3721 tcaatttctt cgacatatgc acataagcta ctaatttgat tatgcatacc tgttaaaaac
     3781 tctagtgtct ctttaaaacc ctcagctagt attgaatggc tagcaatgat caatcttctc
     3841 ataattcaaa tccttttcct ttacgctaat attccaaatg cagccataac catcgcaaaa
     3901 acaataacga gtagaatcaa aatggtcata ctgatttttt tcttactgag tagataatat
     3961 gctgcagcag taataattac tggaattaat gttggtaaaa ttttgtccaa cattggttgg
     4021 attttcatac taacttcacc aaaagtgaat ttaagtggcg ttgaaacact gataactgaa
     4081 gcaattaatg atccaactac tgttagcccc aatactgaag cagcttcagt taaataatta
     4141 atcttttcac cgtattcagt aacaatcttc attccctgct tataaccaaa ttcaaagcct
     4201 ctcattcgga aaaagaagat taccatgatt aaaatgaacc ataaaaagat tcctacagga
     4261 ttattttcta aagccatata agccgcaatt gatcccatga tggttgggat catcgtcatc
     4321 aaaagagagt ccccaatacc agcaattgga cccatcgtac caactttaaa gtcattaatc
     4381 gcatcttttg cttctaatcc ttgcttttcc tccattgcaa cacaagcacc taaaataatg
     4441 gctgccagcc aaggctgtgt attgtaatac tgaaagtgat tatttaaagc ttcgatgtat
     4501 tcatcatcat ctttgtataa ttttcttaaa ataggtgaca atgcgtaagt cacagatgca
     4561 ccttgctgtg tttgataatt aaaggtacaa actgacatca accaacgata gttcgctttc
     4621 ctaagatcct ttttcgttac ttgatatttt tcgctcattt tattcatcct ccccaataaa
     4681 cccaacatca gaagctcccg cagaagcagt gatttgtgca tctttttcag ctctatgata
     4741 cacattaaat gctaaagcga atcctatgat cgccgctcca ataataggta ccttcaaaaa
     4801 agcggctaag aaaaatccaa caagtaaata actaatgtat cgacctgtag gcataaagcg
     4861 taacaacatc gcaataccaa cagcaggcaa catttttccg gctaatgtta gcccatccat
     4921 aaaccaatta ggtaaaacat ccaaaacgta attaacagca gactgtccta aagtaacggc
     4981 aataaatact ggaattgctg cagataaacc aaagaaaatt ggccccaacc acaaaacact
     5041 attcattttc ttaaattctc gtttattcgc atattcctgt gatttgcgaa caacaaaacc
     5101 atttaagatt ttgacaatta catctagttg cacccctaac atacctacag gtagtcctat
     5161 tgctaatccg gcttctactc caagctggct accaaatgcc gttgcaataa ttgcgcacgt
     5221 tccatagtcc ggtacagatg agccaccaaa accggcaact cctaatgaca tcaattgaac
     5281 tgtaccgcca actatcaaac ctgtttttat atcacccatc aaaattcctg tgaataatcc
     5341 ccaaaaaact gcgtaattta caaagatcat cggaatccca taataatcaa catgtttaat
     5401 aaatgctaat atagtcagca taataatttg aaaaatagta aaatgcatta tccttactcc
     5461 tttatcctaa ttttgattga atttcctgtg ccttatccgc cggaacaaat tgtgtaacaa
     5521 tttctacacc agaatccaac aattttttat acgtgctgat attttcatct gatgcatagg
     5581 ctctagaaga tagctgtaat gcattatctt taacaacagt accgccaaca attagtcggt
     5641 caattctaat accgttatct actaaatgaa ggattgtttc cggctctttc acgatgacaa
     5701 atactttttc ctgatcgtat ttcccctttt taaaattcgc aatcgctgtt tcttcagtga
     5761 taattgatac agccattcca gcaggtctag ctaaacgcat acttgatttt tggatgtcgt
     5821 tatttgcgac agaatcatcg atcaccatca ctctgtttgc tttagacatt ggtgcccatt
     5881 gcgtgacaat aatcccatgc aataaacggt tatcaactct cgacataaca atactcataa
     5941 atacttcctc ctttatcttt cattttctta caagcaatta ccatgccaaa acaaaaagaa
     6001 cttgataaat caagctcttt ttacataatc ataaacgtat ttaacctcat gaatcgggat
     6061 gtcgacacta taataatgtt caacgacact gaaagattgc ttaaaatgtg tctgaaattt
     6121 ttgaaattcc tcactcgttt cattgaaata aatttcatct tccaaagtat tttgttttac
     6181 taatcgttca attaagcaac taatgtgaac atataatccg aaacacgtat tattatcaaa
     6241 tgaaattcct agatcatttt gtaagatgta tatagcatct gaaacccgtt ctaataattt
     6301 agtcggattt aatatggtca attgctccat taaattcgtt agactaaaat tcttcagaat
     6361 attattgttc aaaactttca gttcattatc attaaagtgt ctattca
//
LOCUS       DANKHL010000159         6262 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000159 DANKHL010000000
VERSION     DANKHL010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.168"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(41..229)
                     /locus_tag="PW101_002239"
     CDS             complement(41..229)
                     /locus_tag="PW101_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDL2514628.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            499..861
                     /locus_tag="PW101_002240"
     CDS             499..861
                     /locus_tag="PW101_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2514629.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            913..2595
                     /locus_tag="PW101_002241"
     CDS             913..2595
                     /locus_tag="PW101_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDL2514630.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2712..4748
                     /gene="recG"
                     /locus_tag="PW101_002242"
     CDS             2712..4748
                     /gene="recG"
                     /locus_tag="PW101_002242"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDL2514631.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4797..5798
                     /gene="plsX"
                     /locus_tag="PW101_002243"
     CDS             4797..5798
                     /gene="plsX"
                     /locus_tag="PW101_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDL2514632.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5920..6162
                     /gene="acpP"
                     /locus_tag="PW101_002244"
     CDS             5920..6162
                     /gene="acpP"
                     /locus_tag="PW101_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL2514633.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2079 a         1091 c         1427 g         1665 t
ORIGIN      
        1 aaaaaccgtt cactatctga acggtccaag tatcattcaa ttaaacgcgt tcaacttttc
       61 ctgatttcaa agcacgagtt gacacccaaa cttttttagg ttttccgtcg accataacgc
      121 gaactttttg taagttaggt ttaacagtac gttttgttga gttcatagcg tgtgaacggt
      181 tgtttccact ttttgtcttg cgacctgtaa agtaacaaac ttttgccatg gtaatttcct
      241 cctttgcctt gatttggagc aatagaataa ttcagctcat accagactaa catatcatat
      301 agaatggaaa tttgcaagta cattttcata gatacaattg acaattgaga agaataattg
      361 taaattaaac aaggttatac ctgaaatttt gcaaggtttt tgagtacttt gggtaactgc
      421 cttttatttc aagttgcgct atgataaaat atgatagaag aaaaatggga gttttttacc
      481 cgttaaggag gctcattcat ggctgtaaaa attcaaacgt cagctggaac cattgaaatt
      541 accaatgatg taattgcaac agttgttggt ggcgccgcga cagatgtgtt cggtgttgta
      601 ggaatggcca gcaaaaatca aattaaagat aatattactg agattttgcg taaagaaaat
      661 tactcaagag gagtagttgt ccgccaagaa gataatggta ttgctgtaga tgtttatacg
      721 attatcagct atggtacgaa aatctctgaa gtttcccgca acgtccaaga aaaagtgaaa
      781 tacaatctag aggcattatt aggcgtaact gccaattcag ttaatgtctt tgtacaaggt
      841 gttcgtgtac tgccagacta ggcaggagac tggctggtaa ctcagcagtc gatctgaagg
      901 aggattatct aggtgagtgt aacagaaata agtgcaagcc agttccagga aatggtccag
      961 gcaggtgcga atcgtctgca agtaaatgca gaatacgtca attcattaaa tgtattccct
     1021 gtgccagatg gcgatacggg aacaaatatg aatctatcta tgactagtgg agctaaggca
     1081 gtagtagatt caacctcaga aaaagtagga gaattagcag caatgctttc aaaaggattg
     1141 ctgatgggtg cgcgcggaaa ttccggcgtt attttgtctc aattgtttcg tggattttct
     1201 aagcaaattc cagatgttgt ctcattaaat gcttccgatc ttgctgcagc cttcactcat
     1261 ggagttgaaa ctgcgtataa agcagtaatg aaaccagtag aaggaacgat tttgacagta
     1321 gctagagaag cagcgaaagc tggagaaaag aaagcaaaaa gtactgatga tgtcgttgaa
     1381 gtaatgacag ctgttgtaaa aggggcaaag cgtgctttag ctaaaactcc agacttgctt
     1441 ccagtcctaa aagaagtcgg tgtcgtagac agtggagggc aaggactgct gtttgtttat
     1501 gagggctttt taagtgcatt gaatggagaa ttccaagcgg atgaaacata tgaaccttct
     1561 ccagcagaaa tggatgatat ggtcaatgca gaacatcatc gcagtgtcca aggtcaaatg
     1621 gcgacagaag atatccattt tggttactgt acagaaatca tggtaaaaat cggagaagga
     1681 cctacagtcg atagtacatt cgattacgaa gaattccgta actatttaga tggtatagga
     1741 gattctttac ttgtggtcaa tgatgacgag atcatcaaag tccacgtgca tacagaaaac
     1801 ccaggagaag tcttgaatta tggacaaaaa ttcggttcat tagtcaaaat caaagtcgac
     1861 aacatgcgct tgcagcatga gacgattttg gaacacgatg aagaagtgaa cgagtttgcg
     1921 aaaaaagaaa caccacgtat cccgtatggt atcgtagcga tcgctgctgg taaaggcgtc
     1981 caagagctgt ttgaaagttt aggcgctaat tatgttatca gtggtgggca aacaatgaat
     2041 ccaagtacgg aagacattgt taaagcgatt caagaagtaa acgcagataa agtgattgtc
     2101 ttgccaaaca ataagaacat cttcatggca gctgatcaag cagcagaagt agcagatatc
     2161 ccagtagcag ttgttccttc aagaacgatt tcccaaggat tgacagctat gctttcattc
     2221 aatgatcaag cttctttaga agaaaacaaa gaaacgatga cagaaatgtt gtctagcgtt
     2281 gtgagcggtc aagtcactca cgcgatcaga gatacctcaa ttgacggtgt agagatcaaa
     2341 gaaggagact ttttgggcat gatcgatgga aaaatcctga tctctaaccc agatatcaca
     2401 gcaacagcgt taagtacact agaacaaatg atcaatgagg acacagagat cgtaacgatc
     2461 ttggcaggtg atgaaggaac agttgaacaa gcaaatgaat ttgctgagaa actaacagaa
     2521 aaacatcctg atttggaaat cgagatccac caaggagatc agcctgtcta tccttatcta
     2581 ttttctgctg aataatttct gtgtcagaat atctttctaa cacagtattc attcataaaa
     2641 ggccttcggt tagtgatcag gtatgatcgc tgaccaagtc ctttttttgt taagggaggg
     2701 aggaaaaatt tatgcgttct ttagatgatc caatcactat gctggcagga gtcggagaaa
     2761 aaagagcagc tagtcttgcc agcttgggca tccaaacgat tgaagactta ctgacttatt
     2821 atcctttccg ctacgaagat attcaggaaa gaaatctaaa cgagatccaa gatcaagaaa
     2881 aggttacttt gaaaggaatc gtagtctcgc ctcctgtgat gaatcgattt ggttataaaa
     2941 aaagccgatt gcaatttcgt atgatgcaag aacatgatgt gttcaacgtt tcttttttta
     3001 atcaacctta tctaaaagaa aaagtcattt tatcggagga aatcgccgta tacggtaaat
     3061 gggatgcaaa gcgtaaagca ttgaacggga tgaagatttt aggttctcaa acaatggatg
     3121 acttttcgcc gatatatcac gtcaacaaat caattcgtca aacgacatta gttgatctaa
     3181 tcaaaaaagg gtttcaagaa tacggcacat tgattgaaga aaatctgcca aacaaccttg
     3241 tggagaaata tcgactgctt gaccgagctt ctgctgtaag aagtatgcat tttccaaaag
     3301 accacgaaga aaaccatcaa gccaaacgac gtgtcgtttt tgaagagttc tttttatttc
     3361 aaatgcgtct tcaaggattg aaaaaagcag aaaaagcaga aacgaatggt ttggagatcc
     3421 tttatgatgt ccaaaaattg aaagaattta ctcaaaagct accttttgaa ctgacaaatg
     3481 cacaaaaacg tgtaacgaat gaaatctgtc gggatcttcg cagtccgcag catatgcagc
     3541 gacttttgca gggagatgta ggtagcggaa aaacagtggt tgccgcgatt gcgttatacg
     3601 ctaccgttac agcaggtttc caaggtgcgt tgatggtgcc tacagaaatc ttggcacaac
     3661 aacatatgga aagcttgcaa cagctgtttg atcccaatga agtaaccatc gcattgctta
     3721 cggggtcaac gaaagcaaaa gaacgtcgtg agctccttga aaaactagag caaggggaaa
     3781 ttaacatcgt gatcggcaca catgcattga tccaagatgg agtcgaattt cagaatttag
     3841 gtctagttat tacggatgag cagcaccgtt ttggcgtgaa ccagcggaaa gtacttcgtg
     3901 aaaaaggctg gcgaccagat gtcttattta tgacagcgac tccgattcct agaacattag
     3961 ctattacagc atatggtgag atggatgtat ctatcattga tgaacttcca gctggacgta
     4021 tcccgatcga aacacgctgg gtaagaacac ctcaattgga cagtgtactg gattggacga
     4081 aaaaagaact ggcaagagga catcaaatgt atgtcatctg tccgctgatt gaagaatcag
     4141 aagcattaga tgtaaaaaat gcagtggaaa tctatgaaaa acttcgtgaa ctatttgcac
     4201 caaaatatga agttggcttg ttacatggta aaatgaagaa tcaggaaaaa gaagtaatca
     4261 tggaagcctt caaagacaac cagatgcaga tattagtttc gactactgta attgaagtag
     4321 gggtaaacgt gccgaacgcg accgtcatgc tgatcatgga tgcggatcga tttggattgg
     4381 cacagctgca tcaacttcgt ggacgtgttg gacgcggaag tgatgcttcc tactgtatct
     4441 tagttgctaa tcctaaaaat gaactaggtg tggaacggat gaaaatcatg acagagacca
     4501 ccaatggttt tgtactaagc gaaaaagatc tggaattacg tggtcctggt gaagtatttg
     4561 gcttccgtca atctggtctg cctcaatttg cgattgctga cttggtgacg gatggaaatg
     4621 ttttggaggt agccagagaa gaggctgcag ccctttggca gataaaggat tggcagatgc
     4681 ttcctgagta tcagccttta gcgcaaagcc tccaaataga aaatcaggat aatcgttatt
     4741 tcgattagca gactgtgcta tactttatac tgatgacgga aaaaggagtg gaaacaatga
     4801 aaattgccgt agatgcaatg ggcggagatc acgcacctca agcaattgta gaaggcgtgg
     4861 ctttagcgca gcaagatttt ccagaagtag aattcttact ttacggaaaa gaagcagaaa
     4921 tcaagaaata cttaacaaat gaaaaaaaca tcacgattat ccatacagac gaaaaaatca
     4981 atagtgatga tgagccagta aaagcaattc gacgaaaaaa gactgcatca atggtccttg
     5041 ctgcacaagc agtgaaaaac ggtgaagcag atgctgtatt ttcagcaggg aatacaggcg
     5101 cgctactagc agcagggttg ttcatcgtag gacgtatcaa aaatatcgaa cgccctggct
     5161 tgatgtctac cttgccagta gtagggaaag aaggtgccgg tttcgatatg ctggatcttg
     5221 gcgctaacgc agaaaacaaa ccagaacatt tgttgcaata cggtatttta ggttcttttt
     5281 atgcacgtaa agtccgaggt atcggacgtc ctcgtgtcgc actcttaaac aacggaacag
     5341 aagcaacaaa aggtagtgaa gtgacgaaaa aagcctatga actccttcag aatgaaacaa
     5401 gcttgaattt tattggaaac gtagaagctc gtgagctact caatggtgtg gctgatgtag
     5461 tggtcacaga tggatttaca ggcaacgctg ttttgaaatc tattgaagga acagcaatga
     5521 atatgatgaa tctattaaaa tctgctattc taaatgaagg gatcaaagga aaaatgggcg
     5581 ctatgctttt aaaagacggc ttacgttctt tgaaagcaga aatggattat tccaaacatg
     5641 gcggtgccgt tttgtttggc ttgaaagcac cagtcattaa aactcatgga gcaacaggcc
     5701 ccgatgctgt tcgctatacg atccgccaaa tccacacaat gcttgagaca gatgtagttg
     5761 gacaattagt cgaacaattt gaaaaaagcg aagaatagta tttggatatc tgaagcaatc
     5821 cgttccattg aaaaaaaggt ggacattcct gaaaaatgcc agtaaaatta gccatggtgt
     5881 aggtaattga caagtgtatg attcttggag gtgaaaaagt tgactcgcga agaagtattt
     5941 aacaaagtag cgaaaattat ttcaaatcat tttgagatag ataccgataa agtgacggat
     6001 gaattgaaca tcaaagatga tttaaaagct gattcaatta gtattatgga attcgtttta
     6061 gagcttgaag atgaattcgg tacggaaatt tcagatgaag acgctgaaca aattgaaaca
     6121 gtcggtggcg cagtagatta tatcagcagc catcttaaat aatttcaaat aaaagcaaag
     6181 aaggataaaa agctgcatac agctttttat ccttcttttt ttaatctttt tgacacgtaa
     6241 aagtcatttt tctgataatt ct
//
LOCUS       DANKHL010000160         6252 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000160 DANKHL010000000
VERSION     DANKHL010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.169"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(99..1229)
                     /locus_tag="PW101_002245"
     CDS             complement(99..1229)
                     /locus_tag="PW101_002245"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDL2514634.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="PW101_002246"
     CDS             complement(1878..3080)
                     /locus_tag="PW101_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2514635.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="PW101_002247"
     CDS             complement(3140..3343)
                     /locus_tag="PW101_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HDL2514636.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="PW101_002248"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="PW101_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="PW101_002249"
     CDS             4880..5692
                     /locus_tag="PW101_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2514637.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1851 a         1284 c          996 g         2121 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gt
//
LOCUS       DANKHL010000161         6248 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000161 DANKHL010000000
VERSION     DANKHL010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.170"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..2008)
                     /locus_tag="PW101_002250"
     CDS             complement(<1..2008)
                     /locus_tag="PW101_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2514638.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
     gene            complement(2024..3020)
                     /locus_tag="PW101_002251"
                     /pseudo
     CDS             complement(2024..3020)
                     /locus_tag="PW101_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
     gene            3218..4459
                     /gene="sstT"
                     /locus_tag="PW101_002252"
     CDS             3218..4459
                     /gene="sstT"
                     /locus_tag="PW101_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDL2514639.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4606..5241
                     /locus_tag="PW101_002253"
     CDS             4606..5241
                     /locus_tag="PW101_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL2514640.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            5432..6172
                     /gene="truA"
                     /locus_tag="PW101_002254"
     CDS             5432..6172
                     /gene="truA"
                     /locus_tag="PW101_002254"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL2514641.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
BASE COUNT         1968 a         1001 c         1218 g         2061 t
ORIGIN      
        1 ttttttgtgt cgcccatgtt gcactgtact ggttacctgc aacaagatca tattgtatca
       61 gacgataatc ttctagaatt tctttttgtt tctcagttaa atttttttct tggataattt
      121 gactatcctc tgtaacaaag acctgttttc cattaaaacg attagacaag ctttctgtag
      181 gatcaattgt taatgcaggt agatgattag atacttctgt caacaacgca tagtaaggag
      241 ttactttgat attcgcatgc tcaagagcca tagcaccaaa gttattagga gcaacaactt
      301 ctttatttaa cgtagctgct tgttcacgac tatacttgtt gttataaatg aaataatctg
      361 tctggtgctg ttctaatcca tattttttcg aatcattccc actatagatt gcgggtaaat
      421 ggtcaccata aaaaacaaag gtaatcggtt tatcgatttt gtcgatttct tggatgaatt
      481 tttttactgc tgtatcggta tagtgaaggc cttgcatata agtttgtaat tcttttcttc
      541 tattaggaat gacagcagtt ccttcagcag tgtagttcaa ttcactatag taatcatcat
      601 aaggcatatg gttttgcata gtggataatt ggatgaattg tgacgtttct gtattgcttt
      661 gtaaagcttt caaggtttct ttgtatgctg aatcatccga tatgcgtggg ctttgttcta
      721 ttttatccgt ataagttaat ttatccggac tgtctaaata atagaacctg tcaaaaccaa
      781 atttatcaaa cacttgtttt ctgcgataaa gagcagctgt aaaaggatgg atggctattt
      841 tttcatcgaa tagatcggta atatttggac ttattttctg acgctccact agctgggtat
      901 aaggtgtaac taacgaagca gacaggttac tgatactcaa gcctgtcagt ccttcccatt
      961 ccatgttagc tgttccgcca ccataaccga cggataacat caatcctcct gttccttctg
     1021 ttataatgct tttgatgtaa ggaatagggt ctgtaggtac tgtcagatta gggacacgtg
     1081 caggatcact gaagctttca cttaaattga gaacgatcgt ctgattgttt aaccagtcac
     1141 ttcttgtttg gttgatttca tctgcctttg tgttgtactt tttcattatt ccattaattt
     1201 tttctttcga atagccagca ggttcttcca tgattttaat gtcgatattg tttaggaatt
     1261 gaatgactgg tccgttatct tttacggcgc cttgctggtt aaagaaagtc ttgtttactt
     1321 taaataaatt aaacagcaag aaagaaggcg aatttttgtg attaatgaaa aagacacccg
     1381 aaaaacatcc taataataca gcgatactga taaatcgacg tttccaatga atcttcaagg
     1441 aatactgttt ttgtagtcta cgttgtacaa taatactagt aatcaataat aggagaagga
     1501 tcaatccgcc cacgattaat aagactgggc taaccatcga taatagttct gacaatccgg
     1561 tcagtatttt taaatctgaa ggaaagacag gctcttcccg catttccatt ttgattactg
     1621 tcgaaatagt gagcagtaag ttaatgatca aggtagcagt caaggcgtac caaaaacgat
     1681 tggaaatcaa caatagtaag aagaaaaatc ccataataat gatgattgtc aggatcatgg
     1741 gtgcttgtcg ctgagtaaat atttttaagg caacctggat agtttctttc caaccttctt
     1801 ttggttctaa aacgaaaatc tgcacgaagg taaagcagta aaaaaatgct gcaactaaaa
     1861 aataactttt atgaacaaaa agccaatgaa ttcttttttt ccaacgctga gttaattgtt
     1921 caatcgaatc aaaagcgaag tattcgatga aacgatgaat gaaacggatt ttttgtatat
     1981 aaaataaaaa gataactagg agggacactg ctaataaata gcaaataaaa aactgcccga
     2041 tatttatcag ccatgccttc cctgagtttg gaaattcacg tttcataaat gtagatacgc
     2101 gataagaaac taaagggaaa ttacatataa cttgagtgac aatcaaaaat acagcaagaa
     2161 aatttcctct tccttttata cctaatttat cagatagcat ttctaaaaat aaaaacgaag
     2221 agaaagtata atacattgcg aagagagaat atggtttaga aaacctcagc gctgtatctg
     2281 tatttcctct gatgatgagt gaaatttgtg tcatcaaaat aatcaaaccg aataaaacaa
     2341 tcaagctgat aaatacttga ggaagcttat agcggacctt ttctttccag ttccattctt
     2401 tcaaaccata acccattaaa aataaataaa aaatccataa aatactctgt ccattctgta
     2461 atgcaaacgg gtctttatta aatagagtag agcagccaac taatccaatt gttaaaaata
     2521 atagtagata tttaattgtt gttttcgata atttatttaa aaatccaact gtataaggaa
     2581 tactacaaaa aagcaaagta caagaaacag cataagtaaa gtaattctga ctgataggtg
     2641 ttaggataag ccaataatca cgaatattca agctgccagc ttgtacgtaa aaaaaagata
     2701 ttgcaaacta gtgtggctat taggaatata aaggcaaaac gacttaaata atgtaaaact
     2761 tttaaacggg cgattttttg atattggtaa cccatgtaaa gtgggatcaa atttgcacca
     2821 ataacaacgg aagtttgtaa aaatgtatag atgtagaatc gaccagggtt tgttgcatat
     2881 gtaggaatag tgactgctga aatcaatata cgctgtaaaa ccatcattat acaagcgaca
     2941 aagattgtca ggaagccata actctccttc tttatttttt tcaagaataa acacctcaca
     3001 aaaaaattgg acaaaaacat aagaaaacgt ttcctgataa gttgcccatt aataacatcc
     3061 taacttagaa tatctcaaaa aacaacgtgt agacagaaaa agtacaaaat tagcaagaaa
     3121 tggttgtttg tattgttaga atttttagaa aattatacct ttagtttctt tgcataaaat
     3181 gatacaatga ttgttaaaat tgtgaaaggg tggtcatttg ttagagaagt ttttggatat
     3241 gtcgttagtt caacgtattg ttgttggtat tttagtcgga atattgtttg gtatcttttt
     3301 ccccacatgg acatttattt caatattggg aacgttgttt gtcagttgct tgaaggcaat
     3361 cgcacctatt ctggtttttt tcttgacgat ggcgtcaatt gccaagcata aaattgggaa
     3421 taagacattc gtgaagccta ttttgatctt atatttagtg ggaacattgc tttctgcact
     3481 tgtagcagtt attgtaagtt caatttttac tgttcctatt actttacaag aaacagtatc
     3541 tgaaaaagca ccgcaaagtt tggaaagtgt tttatctact atgttgacga atgtcacaca
     3601 aaatcctgtt caatctctta ttgatgcgaa ttaccttggc gtcttattct gggctgtatt
     3661 gttgggacta gcttttcgag cacgttcaga atcgacaaaa gagttggtgg atcagatatc
     3721 tatagcgttg tcacaagtcg tacaagtgat tattgcattt gctccgatag ggattttagg
     3781 attggtttat caatcaattg ctacgactgg aatagctgga ttggcagagt atctgcagct
     3841 tttacttgtt ttgatcggta cgatgctgtt tgtcgcatta gttgtgtacc cattcctcac
     3901 gtttttgttt attaaagaaa atccctatcc attgattttc ttttgtttga aagaaagcgc
     3961 acttcccgct ttctttaccc gaagttctgc cgcaaatatc ccgataaaca tgatgttggc
     4021 tgaaagattg aaattgacaa aagagtctta ttccatatcg atcccgttag gtgctacgat
     4081 caatatgggc ggtgccgcga ttaccatttc cttgatgacg ttgactgctg tacattcttt
     4141 agggattgaa gcaccttttg ttttgaaagt ccttttatgt attttgtctg ctttagctgc
     4201 ttgtggagct tcaggtgttg cgggcggttc actgttattg attccattag catgtagttt
     4261 gtttggtatc tctaatgatg tcgcgatgca aatcgtcggt attggattta ttatcggtgt
     4321 tatccaggat tcaatggaaa ctgccttaaa ttcgtcaagt gatctgttgt ttactgcaat
     4381 aggagagcta ggcgcgcgta aaagaagtgg ggaaaaaatc aaattgaaag attgtttaga
     4441 tggattaagt gaacgttgaa cgttagatat ttagtatttt taaaatgatt cgagttttta
     4501 atgagttata tagatgttcg tgtaaataga ggtttctgtt aaatcaagat aaagtcgctt
     4561 ataataacaa ttaaaagcat aggtaattgt tattggaggc gtatcatgga ttttttacgt
     4621 aaatttggtt ttttcttttt attattaggc gtgctgagtg attttttgac tccgtacgta
     4681 ttaggattgt attatcctga attagatcaa atgaagagcg tgatcagtgt atttggagag
     4741 gtttccagtc ctgtaagaaa agcgtttctt atttggtctg tggtttcagg gatctttttc
     4801 atcctgtcat tgcctgcatt gtatgctgca tttgtaagta catctcgtgt gttagctacc
     4861 ctagttacag cagccatcgc gttctatggt ataggtgatt gtatctttac aggtttgttc
     4921 agtatcaata cccatgaatc ttcgtggaat gtctcgacat gggtccataa tatcggatca
     4981 ggtttaggtt acagtggttt tttattgttt ccagttcttc ttttcttgat ctatcgaaaa
     5041 aatgggcaaa tggacgtaag ccacttatac cttggactgt ctgtgatcag tttgcttttt
     5101 gcggcgattt atggactggc aagaattcct tctataaatt cttggccatt actgaataaa
     5161 ataggttttt gccaaagaat cagttttttc tttaattatg tgcccattgc ctggttgagt
     5221 atcttgcatc tcaaacagta aaacttgagc cgtcaaattg aacagtcagc ggatacttca
     5281 tccggtggct gttttttgtt tttctgaaat atatatttcc acagttttct tgtatagctt
     5341 ctgttttaga gaagaaagga gctatatttt gtgctacaat cttaaaaagg tagagagaag
     5401 accaaatgga agacagagga gccactaaaa tatgagaaac atcaagttga caatcgaata
     5461 tgacggaaaa agatattcag gatggcaaag acttggagat gacgataaaa ccattcaagg
     5521 aaaaatcgaa aaagtattgc atcagatgac aaatgaagag atagaaatta ttggatcagg
     5581 aagaacagat gctggcaccc atgcgcgagg acaagtagca aattttaaaa caaacacaga
     5641 aattggactg gcagaaatga tcgactttat gaatcgttac cttccgcggg atattgtcat
     5701 caaaagaatc gaagaaatgc ctgagcgctt ccattcaaga tataatgcgg tgggcaaaaa
     5761 atacagctat tatgtttgga acaacgtgat tccttcagca tttgagcgaa accacagctt
     5821 ttatttccct caagagttgg atatggacaa gctgaatgca gcttgcgaaa aattagtggg
     5881 tacacatgat ttcattggtt tttctgcatt gaaaaaatca aaaaaatcca cagtgcgtac
     5941 gattgatgaa ttatctatcg aacgaaaggg agaaatgctt cattttactt ttgtcggaga
     6001 cggattctta tataaaatgg tgcggattat catggggaca ttactagaaa tcggcacagg
     6061 tgccatgccg atagaaacaa tcgatgaaat atttgaaagc aaagtgcggc aacatgcagg
     6121 agaaaccgtc cctgcacaag gtctgttttt ggatgaagtt tattatcgat aaaaatactt
     6181 tgataatgga tgaaacgcat gtttcacagt ataaatatcg atgttaaata atgtatgaag
     6241 caaattaa
//
LOCUS       DANKHL010000162         6151 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000162 DANKHL010000000
VERSION     DANKHL010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.171"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..182)
                     /locus_tag="PW101_002255"
     CDS             complement(<1..182)
                     /locus_tag="PW101_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HDL2514642.1"
                     /translation="MNAFKTALNNNEMEGKIIGADCSELAPALYMCDEKIVLPKVSES
                     NYIEELIKVIKFFEIK"
     gene            complement(236..2053)
                     /locus_tag="PW101_002256"
     CDS             complement(236..2053)
                     /locus_tag="PW101_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2514643.1"
                     /translation="MFILTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVSIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFSSLSASAASSILILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNR"
     gene            complement(2067..2831)
                     /locus_tag="PW101_002257"
     CDS             complement(2067..2831)
                     /locus_tag="PW101_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HDL2514644.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMTASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPDYEEVKKKKFGLF"
     gene            complement(2859..3557)
                     /locus_tag="PW101_002258"
     CDS             complement(2859..3557)
                     /locus_tag="PW101_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HDL2514645.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3569..4348)
                     /locus_tag="PW101_002259"
     CDS             complement(3569..4348)
                     /locus_tag="PW101_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HDL2514646.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4364..5308)
                     /locus_tag="PW101_002260"
     CDS             complement(4364..5308)
                     /locus_tag="PW101_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2514647.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(5354..6133)
                     /locus_tag="PW101_002261"
     CDS             complement(5354..6133)
                     /locus_tag="PW101_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514648.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1589 a         1292 c         1116 g         2154 t
ORIGIN      
        1 aatttaattt caaaaaactt tattactttt attaattcct caatataatt actttctgaa
       61 actttaggta atactatttt ttcgtcacac atatatagtg cgggtgctag ttcagagcaa
      121 tctgctccaa tgatttttcc ttccatttca ttattgttta atgctgtttt aaatgcattc
      181 actagttcac aacgacgtcc acaacttaat attaaaatat tcaattttta atctcctatc
      241 tattcgaagc atttgcaaaa gtgacaatgt ctttagccaa ttgttcatcg tcttgcggca
      301 ggctcttgac aaaatccatc actttttgta ttgagtaacc tttgatgttt cctacaaaga
      361 ttttctcata aactgcttca tcatttcttt ccttatccaa caatagttct tcatatagtt
      421 tttcccctgg acggatacct gtttcaatga tttcgatttc gtcttcgcta tacccgctca
      481 atcggatcat gtttttcgct aggtctacaa tcttcactgg ttcactcata tctagaataa
      541 aaatttcgcc accttttgct aatgcgccag attgaataac taaacgacta gcttcaggaa
      601 tcgtcataaa gtatcgagtc atacgaaagt ctgttacagt gattggacca ccttgagcaa
      661 tttgttctct gaatactgga atcacactac cgcgagaacc aaggacattc ccaaatctga
      721 cagctgagaa tttcgtacag ccttcttcat tcaatccagt cacaatcatt tcagcgattc
      781 gttttgttga acccatgaca tttggaggat tgttagcttt atctgtcgat accatgacga
      841 agtttttcac ttttgctgct ttagctgctt ctgctacatt ttttgtaccg aaaatattgt
      901 ttttgactgc ttcttttgga ttatattcca tcagtggtac atgtttatga gcagctgcat
      961 ggtagacgat atctggatga tattgttcca tgatctcatt gattttctcg cgatcttgga
     1021 tatctgcgat gatcggtacg atttctgtag gacaattttg atgatgcgtg cgtaactcac
     1081 ggtcaatcaa gtagatcgag ttctccccat gtcctaaaag caatagcttt gcaggattga
     1141 attgaatgat ttgccgacag atttctgaac cgatcgatcc tcctgcgccg gtgacaagaa
     1201 tgactttgtc tgtgatctga tctttgatgg attcaatatc cagctttacc tcatctcttc
     1261 ccagtaaatc cactacgtca attgttttta attttgatac gctaattttc cctgaagcaa
     1321 gttcttctat agaaggcatc gtgttgactt tcacacgagc ggattcgact aactcgaaga
     1381 ttcgtcgtaa tttttttctt gataaggaag ggatagcgat cgttaccatt tgaatgtcat
     1441 atttttcaat caattccgga atatctttta atgcacctaa tacttttttt cctgaaagat
     1501 acgtatttct tttatttgga tcatcatcta caaacccgac aacgtggata tcttgagccg
     1561 ttttggatcc aagaaagctg ttgtataaaa tacgcccgcc ttcgccagca ccaacaatca
     1621 atgtattttt agcagaatct gctgatacgt atctcatatt ttttgtttcg atgtagatac
     1681 gccagatcaa tcggctaccg atgatcaata agagcgaaag cagataagca aagataacta
     1741 accgtaaact gtatcgccga ttaatgaaca gcagtatgag gatacttgag gcagcacttg
     1801 cagatagtga actaaaaatt gcaatgatct ctcttaaatt tgtatagcga ttgatccgag
     1861 tgaaaacttt gaataaacta ccataaaata aataaaaacc aatcgacaaa ccgatagaga
     1921 ttaaaataag atccattggt atagatacaa acggcttcat gaatttagct gctgcaatat
     1981 ttgcagcaat taataacaag ctgtccacaa aaaccaatgt ggcaattttt cttcttcttg
     2041 ttaatataaa cactttttaa ttccccctaa aataaaccaa acttcttctt ttttacctct
     2101 tcatagtctg gataactgat ttcatccccg ttgatcagat cctttgcaac ttgcttcatc
     2161 tgtactactt tttccttacc aaagtccttg gcaatctgct catagcactc ttttaagtaa
     2221 aaggtgcgct tagagactcc gtgtgcatca gaagcagtca tctgcacgag attatgtttg
     2281 accattgttt tagctgtttt ctgaatatct ttgccaaata ccccaacata acttggcgca
     2341 gtaagttggg ccaaacaccc catatctagg aacggaagca aatgattcgg gtcttcgcga
     2401 aaatgcgcgt tgcgttctgg atggacgatc actggtgtct ttcctagttc taacaactca
     2461 aaaaaaagcc gttctgcata taacggcact tccagtgtcg gaaattcgat cagaagatat
     2521 gtatcatcta aatctgtaaa taaaatttca tctcggtgga tgtcttcgat gagcgttccg
     2581 gtaatacgta cttcttgtcc ttccaataac gtcaaaggca actgtctttt ctctaattcg
     2641 gcttgtaacg aagcgactag agaaatgact tgtgattttt cattgctgta ttttccatta
     2701 ttgtggtgtg gtgtgcataa aatatgcgtg atgccttgct gaatcgcttt ttcagccatt
     2761 gcaatacttg cttctaagtt ttctgccccg tcatcgattc ctggtaggat atggcaatgt
     2821 agatcgatca tttctcttgt ctcctctctt cttattcctt aatttccgta gtaataacca
     2881 gcatctttgc tatgttctgc tccgttgtat acaacaccta aaactcgtgc ttgtaccatc
     2941 tctagcagtt cttttgcttt attcaatgat tctttacgtg atgtattttc acgtacgacc
     3001 aataaagtgc catccgtttt tgaagacatg atctgtgcat ctgttacggc tactactggt
     3061 ggcatatcga atatcaccac gtcaaataac tgtcttgctt cgtctaggat ctgatccatt
     3121 cttggcgaac ttaacaattc tgatgggttg ggtggcttag gtccactagg caaaatcgac
     3181 aagttctcga ctggcgttcg ttggattaca tctgctacac ttcctgatga actaagtgct
     3241 gtacttaatc ccctggcatt gttcagctgg aatgtcttat agacgacggg tttacgtaaa
     3301 tctgcatcga ctagtagaac tcgttgaccg gatttagcaa aaacgactgc aatgtttgct
     3361 gcagtggtgg attttccttc tccaggacct gatgaagtca cgacaatcgt tttgatttgc
     3421 tgaccagctg cggaggcaaa ctggatattc gtccggatcg tacgatattg ttcggcgatt
     3481 ggtgaagatg ggtttgttaa tgtgatcaaa ctgactgcat gagtttgtgc tgctttttgt
     3541 ttctgtgttc gtgccatttt tcttcctcct agactcttga gcggcttctg cgtgatgggg
     3601 ctgttttatc gtttgtagct tttggtcgtg caggagctgt tcctttttta cggataccag
     3661 cgttcaattc tttcgctgac atttctggaa ctgttcctaa gattgggaat cctaatgcat
     3721 ccgtcacata tttttcatct ttcactgtcc gatcaagtaa ttcgaataag aatgccagac
     3781 ctacgcctac catcattcct aatactaagc caatcactag attcaattta ttatttggcg
     3841 aaacaggtgt cgtattggca actgcacctg aaataatcga gattttatcg acattcatca
     3901 cgtcttttgc gttctcttgg aaaacttgtg aagttgtgtt agcgatctgt tgagcagtca
     3961 ctgcattcgt gcttattgcc tggatcgaga acatttgtga attttgtgac tgattgacgg
     4021 agatcgcatc tttgatttgt ccagcagtca ttttcacatt gtcttctgtt tctaaacgat
     4081 ctttgacttc atttagtact aaatcactga cgatcatatc tttatacgta ttgatcattt
     4141 gcaggttcgt attgacatca ttgacatttg ctgtgtctga ttgtggcagt gtcacgatca
     4201 attgtgcttg tgaactgtat tttggtgtca agatgaaaaa tgttacgatt cctgagatgg
     4261 ccaagccagc aaacattgag atgatgattg ttaccaagtg tttgcgtaat gtttcaaaaa
     4321 tttctttcaa gctgattgtc tcttccattt tttctccact ttcctattta ttctctagat
     4381 tcatttgatc ttttaattct tttttcacac gatcaagttc ttgatcgctg actttttgat
     4441 aggagatatt atcttgcatg aagccttctc cttgcagctg atcttgcttg atcgtaccaa
     4501 atgcctcccg ataatctaaa gcgatttttt tcatttcatc aaagctcatg tctgttttca
     4561 tattcgactc tagagcattc aaaacggatt gatagttgct gacaccgttt aaactcaata
     4621 ctttttttgc tacaccttct acgatctttc tttgacgctc ttgtctacca tagtcaccat
     4681 ttggatcttc ataacgcata cgagaataag ataatgcctg atccccgttc aatgtaattt
     4741 taccgattgg aaagtcatag ccatcttgtg agaaagtgag gtcattattg acttcgatac
     4801 caccaactgc atcaacgagt tcctttagcc ctttcatatt gatagagaca tagtgatcga
     4861 ttgggatgtc tacatatttt tgtagggtat ccatcgccat ggcagctccg ccgaatgcgt
     4921 aagcgtggtt gatcttgtct tttgtgtcac gacctacgat gtcgacatac gtatctcgag
     4981 gaatgctgac gatcgttgtc tgtttgtctt caggactgac tgtggcaacc atcatcgtat
     5041 ctgatcttcc ttgatcgacc cgacctaaat ctccagtatc aatgcccatc aatagaacag
     5101 agaaagattc ttgtttgtcc agatcaactg gtttcgttcg ttctccttgg gttcgttcca
     5161 ctgattcgta agttttctca atagaatgtg agagatccag atataatttt gctcctactc
     5221 ctacaattgc taatactaat acgataagaa ctgctaaaat aatcagaaat atttttttcc
     5281 ctcttgattt tcttcttcgt cttgccatag tttcctccgt ttgctaacct tgcttgaatc
     5341 aacgttttaa ctgtcaataa cttatattgc tatagcccat ttgctgagca acagtttgtg
     5401 cttttgttgc gtatgctgcg cttatttttt cattccggat ctgctctatt tgactgagcg
     5461 ccgtcaaata ttgatcgtaa gtagcggtgg cagtcacttg accatccttc aacatcgtat
     5521 cgaataactg atctagttgc gtgacttgcg cgatctgagt atcagcgaat cctagatatt
     5581 gcataacaac cgttttccaa ggactatctt taaaaaagct catattttca cgtacacgag
     5641 cgacatctgc acttgtcacc ttttctttga tgacgacatc gtctacagct tgctgccagt
     5701 ttgatacatt tttagtaaat aaatcgttga cagctgtctg gatcttcagt tttgattctg
     5761 cactatctaa ctgtgtcacg attgctttct tctcatcttg gattgcaaca gcttctttag
     5821 gcaattctga ttctttgatg tcgtaatcat ctgctgaaag tttgacagag gatagcttat
     5881 ttgacaaact tgtgattgtt tcttctgtta tttcagggtt tagaaaattt ttttcttttg
     5941 tactatacag cttgtctact tcttttgata aagaagtcaa ggtgtctttt tgattggtga
     6001 tggcagattc tgcataattg atccgttgct gatgttgccg ctgattattg atatggtaac
     6061 caaacaaaca gatcgcaata gcacctgcac ctaccgccac agcagtcaat gacttgacga
     6121 atggcttttc catagattca ctcctaagta t
//
LOCUS       DANKHL010000163         6129 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000163 DANKHL010000000
VERSION     DANKHL010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.172"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(55..717)
                     /locus_tag="PW101_002262"
     CDS             complement(55..717)
                     /locus_tag="PW101_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2514649.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            901..2547
                     /locus_tag="PW101_002263"
     CDS             901..2547
                     /locus_tag="PW101_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDL2514650.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            2538..3620
                     /gene="uxuA"
                     /locus_tag="PW101_002264"
     CDS             2538..3620
                     /gene="uxuA"
                     /locus_tag="PW101_002264"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2514651.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(3728..5281)
                     /locus_tag="PW101_002265"
     CDS             complement(3728..5281)
                     /locus_tag="PW101_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2514652.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            5429..>6129
                     /locus_tag="PW101_002266"
     CDS             5429..>6129
                     /locus_tag="PW101_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="procyclic acidic repetitive family protein"
                     /protein_id="HDL2514653.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDV"
BASE COUNT         2035 a         1074 c         1158 g         1862 t
ORIGIN      
        1 gattactcaa aaacgaaggt taattataaa aatagaagag gtcgcaagaa aatgctactg
       61 ctggaaatac tcgggataac gttgagtaac agtttcctgt tctttcagca tcatgtgtaa
      121 gtgcaatccc gtttgaaatc gagcttcttc tggtttttta tcaactaaag ccttgagtaa
      181 atcataatgt tgttccatga tcgtttccca tggaagcccc tgaatggtca ctcgtaacca
      241 gcggaaacgt tctaaatggg tattatggtc atctaaccag ttccaaactt cttttcttcc
      301 tgcaatttcg aaacaagctt catgaaataa attatctgca cggaaaaaag cgcgttcgtc
      361 atttattttg actgcttttt cttgttcttc taaaatcgtt tctagcacta ttcgtgtttg
      421 tgtgtccaat ttggaacaac attcaatcat gatttgctct tccaactgtt ctcggacaaa
      481 gcgagcattt tctgcagaaa caagattaat tttggaaaca tacgtaccac tttgaggtat
      541 cgtatacacg agttcttgct gacgtagttg aatcaatgct tcgcgaatgg gagtccggcc
      601 gatagcaaga tgttcctcca gtgccttttc agatattttc cttccaggtt ctaagtcagc
      661 atagataatt tgtttacgaa tcgcttgata agcttgtgat tgcaagctgc ttttcatctt
      721 tattcctcca aaatcatttc tatgaacgta gtataacaga agaggtctga aaaacaatct
      781 ggtatgtcac attaactagt aaatttgtat aaatagtaaa attttggttg acatagcgat
      841 ttttattgta taaaattcac ttgaatacaa aaactaatat gttagtttag gaggggaaaa
      901 atgattgatt taaacaactg gaggaacttt atccctgaat tatctcaaaa agaagtagaa
      961 atacctcatt ttgaccgtgc atcattaaga gaaaatggac aaaaaaatcc tgtctggatc
     1021 catttcggtg gcggaaatct ttatcgagga tttcatgcag aaatcgctca acgtttagcg
     1081 gatctaggcg aattgtccac tggtgtcatt gtttgtgaaa cattcgatga agaaataatt
     1141 gaaagggctt accaagattt tgatgatttg attcttgaag tggtcatgcc aagcagtgga
     1201 gagttcgtca aacgtatatt agctgcgaca gctgacagtc tgtattgtca tccagattgt
     1261 tctgaacact ttgataagat gattcgacac tttgaaaatc ctagtttaca attagttact
     1321 tttgctatta ctgaaaaagg ttatcgattg aaagatacaa ataataaata tataggaccg
     1381 attgcagtcg atataaaaaa tggtccaaag aagccatcac atacaatgag tattcttgct
     1441 gcgctacttt ataaacgttt tttatctgga gcttggccga ttgcacttgt ctctacagat
     1501 aatttttcac aaaacggtag tcaattacgt acttctttgc taacgatcgc aaaagaatgg
     1561 caaaaggaag gttatgtaca aaatacattt atccaatact tatcgaatga agaaattgtt
     1621 gcgtttccat ggacgatgat tgatcgtatc accccgaatc cttccttgtc agttagcaaa
     1681 atgttggcaa aagagggctt ttcaaatatg tcgattttcc aaaccgcaaa aggaaataat
     1741 attgctccat ttgtcaatac agaggacatc cattatttaa tcatggaaga tcattttccc
     1801 aatggacgtc cgaaattcga aaaagctggg gtattcttga ctgatcgtgc aacggtagat
     1861 aaagcagata cgatgaaggt gacgacttgc cttaatcctt tgcatacgtc tttagctatt
     1921 atgggttgtt tactggggta taggagcata gctgaggaga tgaaagatcc agatttatcg
     1981 ctgctagttt ctgaaatcgg gtacaatgaa ggcctcccag ttgtggagtc tccaggtatc
     2041 attgatccaa agatctttct ggaagaagta attactgtgc gcttttctaa tccaaatatt
     2101 cctgatatgc ctcagcgaat cgccacggat acctctcaaa aggtggcgat tcgttatggt
     2161 gagacgatta aaaaatatat ggaaatagaa gttaaagacc cgcagacact tgtattcata
     2221 ccattgactc tcgctgcttg gcttcgatac cttattggca ttgatgatca aggaaaacca
     2281 ttcatcccga gtccagaccc actattgaca gactttcagc ataagctttc cgatattcaa
     2341 ctgggtacgc aagatacatc acatattcat gagaaaatgg ctcctatttt gtcaaataaa
     2401 gaaatttttg gtgtagatct ttaccaagca gagcttggca gaaaaataga agaaatgttc
     2461 atcaagatgg tagcaggacc gaatgccgta cgaaaaacat tacaggatta cttacaagaa
     2521 catggaggaa atttatcatg gaaatgactt ttagatggta cggatcacag gaagaaaaga
     2581 ttaccttaga tgaaatcaga cagattcctg cggtcaaagg gattgtaggc acattgatgg
     2641 atataccagt aggagaggta tggcccaggg agaggatcaa agaactaaaa gaagaaattg
     2701 agtctcgtgg gttaacacta aaagtaattg aaagcgtcaa tgtgcatgat gccattaaaa
     2761 ttggattacc ttcccgtgag cagtatatcg aaaactataa gcagacaatc cgaaacttgg
     2821 cagaatatgg aatagaagtc atctgctata attttatgcc tgtatttgac tgggtgaaat
     2881 ctgaccttga ctaccgctta gaagacggct catccacgtt agcttttata tctgatgata
     2941 ttccagatga tccaagagaa atcgtcgcgc gcatcgaaca ttccagcaat gaatttgaat
     3001 tgccgggatg ggaacctgag cgcttagctc atatcaaatc cttgcttgaa gcctatgcat
     3061 cagtagacga agaaaaactt cgagaaaatt ttgcctattt tctccaatca atcatcccaa
     3121 catgtgaaga ggtaggggtc aaaatggctg tacatccaga tgatccgcca tactcgattt
     3181 ttggattacc tcgaatcgta aagaatcgag aagatttaga ttggttgtgc cgcatagtag
     3241 acagccctta taacggaatc actctttgta cagggagtat tgcagaagat ccagaaaatg
     3301 atgtatatgg gattttagca gaattttgtc aaagagatcg aatcccattt gcacatgttc
     3361 gaaatattaa atttttagct aatcgagatt tctatgaggc accgcataaa tccgaatacg
     3421 gttcattaga catgtaccgc atactaaaaa caatgtatga caacggattt aacgaataca
     3481 tccgtcctga tcatggaaga atgatctgga atgagacagg aagaccagga tacggcttat
     3541 atgatcgagc attaggtgct gtttatctaa atggattatg ggaagcaatc gaaaaaggaa
     3601 acaaaagtaa agataattag tgaaaaaaac tgtaatttta ttatgtaaat aagaaggatg
     3661 ttcaaattct tgatacataa atccggaggt tgttaataag cattcggatt tatgtatctt
     3721 atttggtcta ggaagtaact tgattttctt ttttcgattg atccatgagt gcttcttctt
     3781 ttaattcaaa gatatattct gagatcctca taatcacttt tttccagtct tctggtgtga
     3841 tgattccttt taaaatatag atttttttga tatgagaagg caattgtttc aaagggcagt
     3901 cgctgataac gatgttgcag ttctccataa aatcattttg tattttgatc gattcatttg
     3961 aaaaaatttg ttctatctta acgatcagat ctttactgat ataaaaatca cgcgtatagt
     4021 tcacaccaat cacaattcgc ggctgttttt tgtagcgatc atacaaatgt actaggtctt
     4081 caaccatcca ttgcacattt tccttttcta aaataactgg taacaagtgt tgattttgtt
     4141 ctctgaaatc catataaaat tgttcaatgg cggtttggac ttctttcaac acgctatgtt
     4201 cttgataaag catgaaagta ggaaggggcg tatttggttg attgaagtta agaaatccat
     4261 tataaagttt atttcgtgcg aatgccaatg agtaaagaat cttttctttt tcaggtataa
     4321 acaaatcaaa tacagtacaa aaggactcca ccatttctct tgaataaaga acatgaggaa
     4381 cgtttgcttt ttttaaaagt ttgtattgct ccaagcttgt ttcttccgat tcgattttcg
     4441 tgatagaaag tcgagctaaa atattcacta agatgcgttg ttcgcgacaa attggcagat
     4501 taggtcgcaa aatatctaca gagggatcaa taaaaaagtc aatatattca tactctgtga
     4561 attctttttg tacgtctttc acagaagttg caggaaaatt ctctttacat atttttaata
     4621 gaaaatataa tttatctatc ataccgctat ttagtcggtc aagtacttct tttttaaaaa
     4681 aagtattcat cataaaagcc actacttgcg gatcttcatc ataaaattct gtagcagtac
     4741 ttgaaaagat gttccaataa atatctaatg ctgcatattg aatttgtacc tcttttccag
     4801 aaaaagactt tctgccatta ttagaaaagg agatagacaa accgtacaat gctaaaaatg
     4861 cattaatctt tttcattttc gtatacaaat aagaggcact atagtttgtt tgctgactta
     4921 aactgataat cgaatttgtc tgacagctga acaaagaagt caagatctga taactgactg
     4981 attcgttgca gtatcttcca aatagacgat aatagcagta agccggatca attccttcaa
     5041 aattcaactt taaaaaaggt ttttgcttat caatatgcag ttctgttaga ttgagacgct
     5101 caaagtcttg ttctaattct ttgatgtata gtaaaaaatt tgattcagac atatggaaat
     5161 gatctgctaa atattgtgta gtaataaaga aacgatgttc agataggaaa agaaaaatat
     5221 ctaattttgt attgcttttt ttcttcaaga gaaacgtttt aatcgcttct tgagtttgca
     5281 tgaaagaggc tcctttttac ttaaattgtt aaaacttgaa aatattactg tttttttcat
     5341 aaatttagac aaaaaaacag taaacatata ggtagaatct ctactatctt atttcttgca
     5401 tacaaaaaaa tcaaggagtg agagagttat gagatattca accatcttaa tatcaacatt
     5461 tgcagcctta agcttatcgc ttactataaa tcagacagtc cacacagagg agatagaaac
     5521 agccaaatgg acagaaacca agtgggccaa cgtcaatcga attacctttc aggatgaatt
     5581 ttcatccttc aattcaggag tcgcaaaatt aagaaaagga caaacagcga ccatcaaagc
     5641 aaaaattgac tattcaggag acttagcacc tatactacaa tcagctgtta catttgagaa
     5701 tgttgatccc gctttgtcga ttggctatga cgagaacact ttgactatcc aacacaagca
     5761 agcgatattt accttcaccg ttacattaaa tcaggacttg ttaaaaccag cgctttttac
     5821 tgtcaaggta tcagatggac taccgaacga tcatcatcat ttaacgccgt atagtcagcg
     5881 gcagacaatt gagcaagatt cagctatcga ctcaggagga gatcttgttg aagaaccaac
     5941 agataaacca gaaaatgaaa ataagccaga agtaccgcct acagaaaatc cagacggaga
     6001 acagaaaccg gaaatagaac ctggagaaga acccgataca gaaactcaac cagagccaga
     6061 caatgaatca aaacctgaaa taacccctgg agaaaaacca gatgtagacc cagaagaaaa
     6121 accagatgt
//
LOCUS       DANKHL010000164         6128 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000164 DANKHL010000000
VERSION     DANKHL010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.173"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            91..1248
                     /locus_tag="PW101_002267"
     CDS             91..1248
                     /locus_tag="PW101_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2514654.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1274..1714)
                     /locus_tag="PW101_002268"
     CDS             complement(1274..1714)
                     /locus_tag="PW101_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL2514655.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            1874..2380
                     /locus_tag="PW101_002269"
     CDS             1874..2380
                     /locus_tag="PW101_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2514656.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            2548..4461
                     /locus_tag="PW101_002270"
     CDS             2548..4461
                     /locus_tag="PW101_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL2514657.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            4466..5467
                     /locus_tag="PW101_002271"
     CDS             4466..5467
                     /locus_tag="PW101_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2514658.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(5669..5875)
                     /locus_tag="PW101_002272"
     CDS             complement(5669..5875)
                     /locus_tag="PW101_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HDL2514659.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         2264 a          934 c         1220 g         1710 t
ORIGIN      
        1 agagggaacg attggttata ttttactgga ttcactttac aaaggtactt atcaatcctt
       61 agaaaatctt agtcaaaaaa ttatataagt ttgcgtacga ttcaaagaaa attaagagaa
      121 cttaatacta ttttgaaaaa ctatcattta tcttgcagct tgaaaaaagg taatccgata
      181 gaaggagaag aataccgtat ccgatacttt tttcacttga tgtattggca gtcattcgac
      241 gaaaatcatt ctgatatatt atcaagtata aaaaataaac gtcaactatt agaaaaattt
      301 gcgaaacatg cgccatatac gcgtagtata gatagaaaga aatggatcag cctactaaga
      361 atttcgttgc aacgaatgaa acaaggccac tcgattacgt atgtcccaga agagatgaac
      421 aaatttgtgc atccattgat aacaaaagaa gagtttacgc attacatact agagcctttt
      481 tttgaagcaa acttctggtc gatgaatcac atcactccaa cagagcaagc ttacctttat
      541 tttatgtttg gtgtaatgaa cacctatttg gaagaagatt tgttggatac tccagataaa
      601 gtacaagaaa gttggggctg gattgaatcg tctattgtag aaaattttga aaaaaacttc
      661 aagatttgtt ttacaacttc agaaaaaaat tatctatatg tcaatttggc aatgatccat
      721 ttgtattcaa gggtttttgg gacaaaaaag aaggtggatg cttttggaaa atcagcagaa
      781 gacagagatt ttcagcatat atttccagtg atgtatccaa tcatgcaaca gttttttagt
      841 ggggtagctc gcagaattcc agagcttaat acttattatg aaaagaacag acggttgatt
      901 tttcaatact gtatgttagt cagggatatt tttataaaac acgaacaacc tgtcatattc
      961 tatattcaat caaagtatgg taaaacacag gaattatggg caaagaaaag gatattatct
     1021 atgactgaac gcccaataaa atatagtgca tccgctgatc agttacctga tattgtcatt
     1081 agcgattatc ctatcgacaa aaaggcatat gatcaaacga atacacagat attttactgg
     1141 aatggacaac ctacaaaaaa tgattgggcg cgtctgaaag ctgaaatcat aaaaatcagg
     1201 aatgagaaaa atagatccat attggaaaaa ttggatttcg ttaattaaga aaagcagatt
     1261 gtttatgcca aaattaagac attgcaaaaa gattattttc tttaaaatcg acaagatgac
     1321 cagaaagcaa gagttcgttg agctcttgct ttcttttagt tttagaaagg aacatcctga
     1381 ttttatcttc gctaaatcca gtcaaaagtt ttgcagattg gtcgtagata atcggttttt
     1441 tcaataaaga aggggatgta cgaatctcaa tatatagttc actcaaactg agttcatcta
     1501 ttctgtgaac aagattttta taactaatag aatgagtaga gagcatttcg gtcacgccgt
     1561 tgtttgtcaa ttttagtaag tggataaatt catttttggt tagtggttga cgaattaagt
     1621 tctgttcgat aaacggtata tcatggacga taagccaacg ttttgcgcgt cttgaagacg
     1681 agcagccaga acctgtatac aatttaatca tcatacattc ctccaatagc taagtattta
     1741 tgaaataata acatgaagaa atagtcgaaa agtgccattt agaggtgtaa aataagaaaa
     1801 tgttgtcacg tataatggaa ctagctagat aagaaatact attaaatcaa gaaaaaagaa
     1861 agaaggcaca agtatgtcaa acacagccga cgcaatagta gtgatcgatt tacaaaaagg
     1921 ggtatgtttt gattcttcgc cgatacacga attagaaaca gtgacagcat tagtgaataa
     1981 aagaatcaaa acatacgaaa cgattgggaa accagttatt ttcgttcaac acgaagatgc
     2041 tcaattagtg ccacaaacaa aaccatggga gctattgtcg gaattatcta ttcctgagca
     2101 cagttatttt gtgagaaaaa cccatgcgaa ttcattttat aagactaatt tgagtgtttt
     2161 attaactgaa ttggactgtc attcgatcga attatgtggg gcacaagcag agtattgtat
     2221 agatacgact gtgaagtttg ctcatggatt aggataccag cttatgatgt gtagaggagc
     2281 aacgacaact tatgataatc cttttatgaa agcaaaggaa acaatcgctt tttacgagaa
     2341 aatttgggat aaacgatttc tgacattttt ggagcaataa gttgtttctt cgtttaaaaa
     2401 aagcagaatg gatactggtt tatccattct gctttattac gtaaaatgag aaaataatcc
     2461 tgttctattt ttatgggata aaccatttat tttttgtata aaaaatgttt gcgtttacat
     2521 ataattctcc tagaaacata aaaaggagtg aatagcttga gaacaaaaca agtattaaag
     2581 ggagcgttgg tcatcttatt tgctgtaagt acaagcgtcc cgcaatggac agtatatgga
     2641 gagagcattt cttccgaatc ggaatctcgg gaggtagaaa ccacagaaac tactgatgct
     2701 ggaaatgaaa cgaaatcgag tacttcacaa acagaaacaa atactgaaac aacggttgag
     2761 acatctgtta ctagcacaga tcaagaaacg accgctacca cagataacca acaaacagaa
     2821 gaagcaacag ctacaaaaat gtcaaacgac gaatcctctt ctgctaaagt aagtgacaaa
     2881 gagaatggga cagaaaatga tgatatcggt aaaggtgaaa atttcaatgg taaagaatat
     2941 ttgaattact tcggtaaaca gatgacagaa atacttaatg gaccaacaga agacatcaag
     3001 tcaaaattaa tagagttatt agtcactgag cttgctgtta atgattatgg tgtagataaa
     3061 gagattatca aacatctgat tgctacttat ctattgtacg atatcgcttt tgcaaaaaac
     3121 agtgtgttga tgtcgatgct tggaggtaca gatgatggga caacgcctac agaaaacaca
     3181 ggcttctatc caaatacaaa acaacttcat caaactgtac taaacaaaca agatggcaaa
     3241 atgatggatg tatatgcata ttatgtcgat caaggttcag ataaaacagt catgatccat
     3301 ggcggatttc gagggaattg gaataacggg atcgttacag aagaatacaa tgatttttat
     3361 aaggccgggt ataatttatt atttgttgat tccagagcga ctggtaatag cggaggcgat
     3421 tatgtgacct atggacaata tgagtcagat gacgtccttt actggataaa tcaagaagtt
     3481 cgagagcggc catctcaaaa gattttactt tatggtggtt cgatgggagc agctacgatg
     3541 atgtctgtat tagcgaaaga tattcctgtc aatgtcaaag gaattattga aaattgcgga
     3601 tttgcatcaa ttgatgaaca actacgtttt acgtacagcc agacagtagc ccccgcacta
     3661 ccggatgcca taaaaaatca gttagacatc attggggatc aagagcatga agatttattt
     3721 atgggccttt tgaaacaata ctattttgat caggagatgc atttggatac aaaagctgct
     3781 ctacctacga ttggaatgtc agattcattg cctaaattga ttatccatgg aacagcagat
     3841 gatgtagtac ctgtgtctaa cgcgcaaaaa ttatatgagt tatcaggagg gtataaagat
     3901 cttttattag tagaaggagc agggcatgga aaggcacagg aagttgacca tgctgcctac
     3961 acgaaacatg taacggactt tttgaaagta gtgttccatg atcagataaa cgtaaagtat
     4021 gtggatgaaa acaataaaag tctactcaaa gaccaggacg aaatccagct ttacggagct
     4081 tatggagaaa attatatgac agagcaaaag acattcgaag gatacgaatt agcaaatgtt
     4141 gaaggaccga ctgaagggat ttttaatgag acgataccaa ctattatttt ctattacaaa
     4201 aaaatcccag tagctccacc aaaaaagcaa gatccgacag agaatgcaga caataaggga
     4261 aagaatgaac ctcgagagga tcaatcaagt gatgatacgc aaccgattca gatgacagtg
     4321 aaagtacaaa aaaagacaga tgcaaaaaaa agaatgttgc cgaaaactgg agaaaagaaa
     4381 cattcgcttc tcatttattc aggtggattt ttatctgtta gcacgattat cacatggatt
     4441 tggcgaagaa agagagggta aatgaatgaa atggttcaag cgaatagtga ttgcagtacc
     4501 agttatcatc gtagttattt tagtcactgt atttttgatt aatcaaatca caccaaagcc
     4561 gattagcttg cttgttagaa aacaatttga cacaagtgaa aaagaacaag tatatgcccg
     4621 accagatgat ttccagcaaa aaatcgaaca aatcgatata aaaaaagaaa tatcctatcc
     4681 ttcaaagtac aaaaataata atatggatat ctacacaccg aaagtggaaa agaaaaaatt
     4741 accgattctt ttctggatgc atggaggcgc atatgtagga ggggataaaa atgattgtcg
     4801 tgattatttg gaatatctat gttcagacac gcagcaaatc atcgtaaata tagattatga
     4861 acgatcaccg gaagcaaaac atcctacacc agttatccaa ttgaacgaag ccattcaagc
     4921 agctaaaaaa gagataaaag atcaagcaga ttggtcgaat atttcgattg gtggaaattc
     4981 ggcaggtgca caaattagtg gagaatattt gttagcactt caaaatgaag aaattaaaaa
     5041 agctgatcat cttgatccga ctttggagaa caagcagatt acgaagttta tttctttgtc
     5101 tggtctgttg gatccttctg cacttactca agtaagtgat aaaatctcta gctttcttta
     5161 tgaaaaatgt ggatgggctt actttgataa taaggacttt gaacaatcag agaaggtcaa
     5221 aagtttagcc ttggtcagac atgcggatcg ttggacgcaa aacacattct taacggatgg
     5281 aaatacaaat acgtttacga aacaaatgga agaagtaact tctgtatttg agaaagcggg
     5341 ggtgaaagtc acaaaagtag attatgcgga gaaaaatgct gttttgaacc atgaatatca
     5401 gtttgatttc agcaataaac aagcgcaaga aacatatcaa aagttagtaa tgtttttcaa
     5461 ggagtaaagt cttatgatgc aaaagaggtt gtaatctgtc acttagctcc aagtcacaag
     5521 gagcaatttc aataaacaag cttttcatgg tttatgaata ttgtaacttg tgactgagga
     5581 gttagagatg gcaaggggca ccttttattc gaaatttggg aatatcacac gattattgtc
     5641 acgatctctt ttacgtaaat tttataagtc agatttgttg ttttgatcgt ttaaaaaaag
     5701 caacaaatcc gaagcctgcg gaggcaagaa gggacaaaaa gccgagtggt aagcaaaaga
     5761 atgctggttc cactaatatt ccgttgttat tgatataagg ctgggaacaa agatgaatag
     5821 caaataaaat caatgcacta gcgaaaaaaa gcaaagaaat atggatagct ttcataagca
     5881 gcacctcctt ttttataatt gtatgataaa tagattaaga atctataaat gtgacaagtt
     5941 tgttaggatg tgaataaaat gatcatctag cgaacttgaa acaaagatgg ctaatgataa
     6001 gcaattaatg attcagcaat cagataggta caaagtttac catcatacgt ctagaccaat
     6061 aatagaaaat atgggaatat aatagaaaag atttaggata cccccaaaag aatttcccca
     6121 aaaattcg
//
LOCUS       DANKHL010000165         6080 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000165 DANKHL010000000
VERSION     DANKHL010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.174"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(256..543)
                     /locus_tag="PW101_002273"
     CDS             complement(256..543)
                     /locus_tag="PW101_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2514660.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(563..787)
                     /locus_tag="PW101_002274"
     CDS             complement(563..787)
                     /locus_tag="PW101_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HDL2514661.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1242..1412)
                     /locus_tag="PW101_002275"
     CDS             complement(1242..1412)
                     /locus_tag="PW101_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514662.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1427..1960)
                     /locus_tag="PW101_002276"
     CDS             complement(1427..1960)
                     /locus_tag="PW101_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL2514663.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1981..2925)
                     /locus_tag="PW101_002277"
     CDS             complement(1981..2925)
                     /locus_tag="PW101_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDL2514664.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3562..3648
                     /locus_tag="PW101_002278"
     CDS             3562..3648
                     /locus_tag="PW101_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL2514665.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3769..4459)
                     /locus_tag="PW101_002279"
                     /pseudo
     CDS             complement(3769..4459)
                     /locus_tag="PW101_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4704..5618)
                     /locus_tag="PW101_002280"
     CDS             complement(4704..5618)
                     /locus_tag="PW101_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL2514666.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5600..5980)
                     /locus_tag="PW101_002281"
     CDS             complement(5600..5980)
                     /locus_tag="PW101_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDL2514667.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1611 a         1123 c         1024 g         2322 t
ORIGIN      
        1 cccccccact ctccacaata caaaaaagac tagcaaatgc tagccacttt ttgtaaagtt
       61 tcgcttgttt caccttcggt gaaattgttg caaatcaata attaggtaga gctacatttt
      121 tttcgttgtt caaagattct tatgccattt actattaata aatctataaa tatgataatt
      181 tttcggctct ataatttatg ggactgatga atcagaattg attcttcagt cccattttca
      241 ttttattcca aagaattaac tccaaatacc aatagaagcc catcctctat atacttgaga
      301 ttccttacta acaatttctt gtaattctat taaagattta ggtaatccaa atttatgttt
      361 taaaatatat cttccaagaa aactacttaa tttcatacta acttcaccaa cactttttcc
      421 ctcactgatt tctttagaat aaagaaagag cttttctttt aagtcaggat agtcatctat
      481 agttgcttca ttgtatactt tactaattgc atttaaaagt ttttcttctt tagtaaaatc
      541 cattataata acctccctac cactaccttc taggtgccca tggaccatga ttaacccaac
      601 cattaacaat aatttttcca atttctcctt tagcttgatt ccaatctacc caacattttt
      661 ctttgttaca ataaagacca tttccatagt aagtagctgc atcaactttt attcctgtac
      721 cgatgccagc tagacaagtt cctaatattc ctagaataac acaatgtttt aatactttct
      781 ttttcatgat tgtttttcct tttcgttttt ttaaaattgc ttgttatatt aaataatctt
      841 agtaatttta ttatataaca ttttattata taacaatgat taacgtcata aaaagtagtt
      901 ttgaattcat aatcgtttaa ataaaattat aagatcagtt atttaaacag ctagtagaca
      961 taatgtgtat actaccaagt aacgcctgta ccaagggatc caggcgctga ttttgtgaat
     1021 ttttattggt ctaggttaaa agctcttttt aaggcttggt tttctgcggt tgtttctttc
     1081 taattctttg tgaatattga tcggtcattt tttaaatact tgttattttt gtttgtgttt
     1141 gttatgcttt ttacatatct catattcatt tattattctt ttctaaggtt aataatagga
     1201 taatctcttt cagaaagact gctcgtacga gcagtctttt cttatttatc aagtttttct
     1261 acaaatactt cctcattttg actgttgtag taattaggat tgagttttag ttcccctttt
     1321 ttatttcctt tgttggccac tgataataac attgggctaa agcttaatat tcctagtagg
     1381 atagaaccga atagcaagtg tttttttgtc ataagtattt ccttcttcat ttccatagct
     1441 gccaccattt tttccaattc tttgtttgtt cgtccattgt ttctgttgct tcagaaagag
     1501 tcgttgaact attctctgaa gactcttccg tataagtcaa ctgcagttgc tcctgaagtt
     1561 tttcgatttg ttggttggct tgcagggtga gttgttgttg ctggtcgagt agtttttgta
     1621 tatgagaatt attttgctct ttgagggcaa gttgttctcg taaaaattca ttttcttcac
     1681 gcaactgatc tagcaacttg tttgcaacca cgtttttgtt tggtatataa gcatttttat
     1741 cgttgttttg ttcatgttta tgcaacttat ctagcaacct ttgattgtct aagttgcgag
     1801 caagttgcaa tactccagaa cgcaatacaa ccttataacc acgctcattt ttagctatca
     1861 tggaagaatc taatttttgg atatgtttcg atattgcttg cctactcatc ttaaaaagat
     1921 ctgacaattc ctttattgta aaggtttctt gtggttgcat actggttgcc tcctaggttg
     1981 ctactggaat aaattatcta aatcagataa taaacttttt ttctcttttt ccgaaagtgc
     2041 ggactctgtt tgtttttcaa gagctttttt ctctttttct agttgtttga tttgttggag
     2101 gagttcaagt tctcgtttct tctgtttgat tttttcgtta attgttgtag cgggttcttc
     2161 gtattctttt tgtttatcag ctaaagccaa tgctttattt tggtcttctt tggttggtgc
     2221 atgtttgtta aagtcgtctg cttttttgat ctcaggtttg aacgtaaatt gatagccaat
     2281 aacaggtttt ccccgtcctt ttccgtattt ctttttgatt gttagtcctt tgaagattgc
     2341 agttagttct tgacgtatag gttttataac tcgtttatct atatcaggtt gcttataact
     2401 tttaggaata tctaataatt caagaaagtc ttctttggag aaatatgcga aacctttggt
     2461 tctgtattgc tttaacagac gaaacatagt ctttgcatag ccactttgta gctcgttgaa
     2521 ttgttgtaaa gaatagcgta cccactcgtc taaattattt aacaaaggca gtgctttttc
     2581 gtaaattcgt atatctacgt aaggattttc tacgtccccg ttgattttaa attctgtaaa
     2641 cattacaaac atatcgtaag aaagcccaga ctcacttcta cgaccaaagc gtaaagataa
     2701 gattttttca taagttgctt ttaaatcttc tacaaatcgt acatttgcag ttgctttgta
     2761 gttacttaaa tcttttagtt ggtcaaacgt aaaacgaact gtcttatccc ctttttcacg
     2821 cattctagac acgatggaaa aaaacaaatt catttcaacc ggtgtaaatt ttcttaaagg
     2881 aattgtattt aattcatggt ggtattttac aatctcatta gccatataat cacctcaatc
     2941 caattatgaa tgacattttt taaatgtcaa gacattcata tttgacgttc aaacagacaa
     3001 aatgtccttc ataaacagac aaaatgtcct tcataaacag acaaaatgtc cttcataaac
     3061 agacaaaatg tccttcataa cattctgtat ccattggtag agtaaggaca cagcttacgg
     3121 taaagaggtt taaaaagagg tttaaaaaga gatataaaaa gaggggggct aattttcccg
     3181 cctaaaaacc acttcttttt tctttaaaca ggttcgcgca tttttcaaaa ctgccaatcc
     3241 aaaaccttgc ttcctacggt cgccaaaaag gtgtgatcgc tacgctctca cattgtactg
     3301 cgcagtcgcc taaaggctcc tttgctaaaa acatgttggt aaagaggcct ttgagcggtt
     3361 ctgaatgctt atctaagccg ttttaagaca gtcttggata aaaatcctta tctagcttta
     3421 aagcgcctta caggcggtca gggcaagccc taacaaccct aaaataagcg atacttaaaa
     3481 acaccacaca agtttaattt tttaagatat aataacttgt cgcctatccg acagtcttgc
     3541 gactggaagg aggtgtatct aatgtttgaa ttgttcattt gcccccttct tatagaagtt
     3601 tgtgtctctt tattcagtta ttggttgaat aaccgaaaca agaagtaaaa tcaataaagg
     3661 cgacatcaac ctgaaaaaaa gccccagaga atccgacctc tctggggctt tcgtgtatct
     3721 aatgtttgat catttgcatg tttagtttaa catgattgcc gatcaaattc aattttgctg
     3781 ttgctgatct aaccattcct tgacactttt tagataattt aatgccgttt cttttttcgt
     3841 tggattttgt atgttcgttg cttgctgaag cagtgcttta taacgttcgt aggtaactga
     3901 ttgttgccgt tcttttcgtt cctgttcact agttgaaaaa gggattgtat cactgaataa
     3961 attataacaa gcttcttctg tctgcgtatc tttaaaatat tgcacgtctt ttttggttcg
     4021 ctcatagttg tcttgaacct ctttttgctg tttttctggc aagttttgat aaagacgagt
     4081 gagtttttcc atgtttttat ccgtcaaacg tttttcttga ttcacaaaaa tatcgttcat
     4141 ttcctcataa caagtggtaa actcttccat tttttgtgtt aacacttgct cctttttctt
     4201 tttatcacat gctgcttcat acttttgagt gatatcccct aataaaatac ttcttgaaat
     4261 acgtttattt ccctctaaaa cgccgaataa accacttaaa acgattgata atgtaaaata
     4321 taacccctgg caataaaaac ctcttaaatc gaattttaga gccttttaat gaatatcttg
     4381 tttgataagc cttatagcta agccaaataa ttcctaaaat actggcaaaa atgagtagat
     4441 agggtaaata gaatgccatt tctatttatt ctccccatac cagccaaaag gctaacagtg
     4501 tcatgattaa gcaacataag ggggcaagaa tatccattga tcgctgcata attacttttt
     4561 tctgttcttt atttcgctgg tttaatagcg catggtggaa ttttttgact tgtgaatcaa
     4621 ttgtagtttg tattctttct ttggtataag gtgcaagaaa agcacgttgt ttctttattt
     4681 cattttctat gtcttgtaac gttttagaac gataattctt gttggtagtc tgctcctctt
     4741 tcaacgtgtt ggtcatggtt tcttcgagtc ggtctaattt ccggttgttc agtcgtaaga
     4801 gttcgttgtt ctcttcgtac ttcttttgca gtttcgcgaa tgctagctcg agctcttcgt
     4861 agctcgttgg ttcgttgttg attgtaggct cgttctcgtc tgtccagctc atgtaaaatc
     4921 gtctcctttt cgtaatcctt tcctagtttg ttggatcgta cttttttctt tttgtggatc
     4981 aattcgtaag taaaattttt cccacgattc aagcaatgaa tgccgaatgt tttaagagaa
     5041 tcacaaaatt ggttatacgt tcgagttttt ttgtttttca ttgtttgatc aatcgcaaac
     5101 cgaatttcgt ctttccaaac aggttcattt ttttctttta atttgatttc agacatggtt
     5161 cgtttttcgg gtttcttccc tcgttcagtg attgttaagt catgttcaag acaaatctcg
     5221 tcatttaggt cataacattt gtttaaaaat gcttgaccat gggcttgaaa tttgttccca
     5281 gtttccagat tgacggagtt aatgacaatg tggttatgaa tatggtcttt gtctgtatgt
     5341 gtgtaaatcg cgatttggtg gtcaggggct atttttttcg catactctgc ccctagttga
     5401 ttggcttttt cagcggtaat ttctcctggt cgaaaagact ggatcaataa gtgtgcttgt
     5461 acacgatcgt cttttccata cagcatgcga atttgtttca tttcgctttt ggcataatca
     5521 atgtcacagt ttatccctgt tttgacggtt gctcgtggtt cggcgtaatt gatcgctcgg
     5581 ctacaagaag tggttcggct tactttaact gttgccatat cttttgtagc tccttttgca
     5641 tgatttttag gttttcggat aagcgttggg cgtcgccagg atcaacgtcg tgttggtgtg
     5701 tgttgcacca cttggcaatt tgattgatat tcccgccagc tttggctaaa tcgcgcgcga
     5761 ttgcctgagc aatgtctggc gccactttcg gtgtcaccag acgcgcgttt tgtgccttgg
     5821 ctttcacgaa agccggcaca ctcatttgca aagcttcagc tgactgcttc agctttgcaa
     5881 attcggattc gctcactcga aaactgattt gttttggctc ggatcgatag gtatattttt
     5941 tagaatgatt gctagccaaa tattgatttt gttcgctcat aagtcccacc ctttcagtac
     6001 gtcctacgga cgtatcgttc gcaataccat tgtacctaaa aaggtacaat aagtaaagct
     6061 cactaaagct cactaaaggg
//
LOCUS       DANKHL010000166         6066 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000166 DANKHL010000000
VERSION     DANKHL010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.175"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(278..763)
                     /locus_tag="PW101_002282"
     CDS             complement(278..763)
                     /locus_tag="PW101_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514668.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(902..1117)
                     /locus_tag="PW101_002283"
     CDS             complement(902..1117)
                     /locus_tag="PW101_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDL2514669.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1341..2141
                     /locus_tag="PW101_002284"
     CDS             1341..2141
                     /locus_tag="PW101_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2514670.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2160..4028
                     /locus_tag="PW101_002285"
     CDS             2160..4028
                     /locus_tag="PW101_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL2514671.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4021..4512
                     /locus_tag="PW101_002286"
     CDS             4021..4512
                     /locus_tag="PW101_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDL2514672.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4500..5054
                     /gene="srlA"
                     /locus_tag="PW101_002287"
     CDS             4500..5054
                     /gene="srlA"
                     /locus_tag="PW101_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDL2514673.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5072..>6066
                     /locus_tag="PW101_002288"
     CDS             5072..>6066
                     /locus_tag="PW101_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDL2514674.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2137 a          966 c         1217 g         1746 t
ORIGIN      
        1 aaggtaaaaa agtaaattct tctatccaaa tatagaaaaa ttgaatctag aaaaccgccc
       61 gacaataaat ttcattaact cagttgaccg aaaatgaagt agataccaaa aatttatcaa
      121 gagagcaagt agagaagtga gtatattcat aagagaaata gctgtaacga attattaggt
      181 taatgcaaat gcttatttat actccgtata actttctcag aaaaaatgat accaataatg
      241 acttgctagt actgtaaatc cgtataatat catctaatca aaatggtgtt ccatttgcat
      301 ctgtcatcgt aatcgatcct tcgggtgaca ctcgataaaa accaacgctt ccagatcctc
      361 cttgggaacg gatttgtttg ctggaagctg tgaacaaata atctcgatca attttttgat
      421 aaaagctaat aacaatatcg tcactttgta attcggaacg gtgggcaata agaaatagaa
      481 gtgctttgtc agagttttct aatggaagag aaggtacagt gctttcttgc atttcagatg
      541 ttgaagaaat gctaaacgac tgaatacttg attggctatg ttgcgaatct acattaccag
      601 tagaagtatt agtaatggaa gtagtagaag tacttttagt tgaaccaacc tcagttgttg
      661 aaatggctgc tgaagattct ttaacagaat tctttgtttt atatccacag gcagaaaaaa
      721 gtattacagt tcccaacaag cctgcgtaaa tccctttatg catgatagct cctcctaaaa
      781 tagattatct ttattcagta tatcacttaa acaaagaaaa gcagatggtt taaaatgaaa
      841 aaaccttccc aataaaggga aggttcatga agataataac ttttttaata attatagctt
      901 tctaatatct ttcatcaact tttctagtaa tcattgggaa aaatttcaag cagtagatcg
      961 cacaaatacc tacgacgata taaatgatct tcgcaaaaat agttgttgaa ccaccagcaa
     1021 tcatagctac taaatcaagt tcgaacaagc ctaccaacag ccagttcaac cctcccacaa
     1081 tcaataaagt taatgcaata gcatctaaag ttttcattag aattctcctt tcttaaatga
     1141 taaaacaatt atatatcaaa aagagaaaca ttgcttatat aacgctcagg aattttagca
     1201 atctatgcaa aaagatgaaa attttttttg ctcttccatt agtacacatt ttttagctga
     1261 agctgtaaat ttcatgtctt aatttttttt agtgagcttt tatactggat ttgtaagaaa
     1321 aaagaaggag aggatcacat atgtcggatt ggttggatat tgcaggaaaa gtagtaattg
     1381 taactggagg ttcctcagga attggaagaa gtatcgtcga aaatttgttg aagcagaatg
     1441 cacaagtagc gaattttgat gtaactgaat gccgtataca gcatgaaaac ctactatctt
     1501 taaaagtcga tgtgtcttct aaaacggaca tagaagaagg gatatataaa gtaatgaaac
     1561 atttcaagac gatcgatggt ctagtcaata atgcaggaat aaatattcct tctcttctaa
     1621 tagatagaaa tcatccaaaa agcaagtatg aattatcaga acaagttttt gataagatga
     1681 tcgctgtcaa tcaaaaaagt gtatatttga tgagccaagc agttggaaga atcttggtgc
     1741 aaaagggatc aggagtgatc gtgaatcttt cttctgaatc aggattggaa ggatcagaag
     1801 gacagagctg ttatgctgct acaaaagcag cgatgaacag ttttactcgt tcttgggcaa
     1861 aagagttagg aaagaggaat gttcgtgtgg taggtgttgc accagggata ctggaagaaa
     1921 ccggtttgag aacacaagaa tatgaagaag ctctttctta tacgagaggt ataagcgtcg
     1981 aacagctgag aaacggttac agtcgaacaa gtacgatccc tctaggaagg agtggtaagc
     2041 tgcaagaagt ggcggatcta gtctgctatt atctttcaga aagatcaagt tatatcactg
     2101 gagtcacgac taatatttca ggaggtaaaa caagaggata aaggataggg gggacgcacg
     2161 tgacgctagt aaacagatgg taccaattat tgaaattact agtcactcac aagaaagttt
     2221 caatccagga agtgcaaaaa gaaatcaaaa ccagtaatca aaccataaaa aaagatattg
     2281 gggagttgaa tgaagaaatt gccggtattg caaaaatcat ccaaaaaaac aaacattttg
     2341 aacttgaaat ttatgatttt gatttttttg atgagatcat gcaaggaaaa ttgaaaacag
     2401 ccagtgattt caattcggca tccaaaagaa tcgcttattt actaaatagg ctgatcgaaa
     2461 cagaggattt cttacgtatg gatgatttgt ctgaagaagt cggtgtcagc agaggaacag
     2521 tggtcaagga tctaaagaca ttaaaagaga tgatcaatga ttttgatgta aagattacag
     2581 gcacaccaaa taaaggacta agaataatag gagatgagct agaattacga ctacttttat
     2641 tttattttgt ttctccctac ttttcagaga cgaaaacaga acagtttcta gaagaaaaac
     2701 aagcaatcaa acattttcag aagaagacga atgctagtaa acaagaaatc acccttttaa
     2761 cgaaagttat cggtatttct ttgaatagaa tctctagtaa ctatctgttg aagaagccca
     2821 tcaatcgtta cagtgggtgt tttgaatata tccaagaatt caatgaactg atcaatcagt
     2881 tggaagaaat ctatgaacta actttgagcc aatatgaaaa agattttatc agttttcctt
     2941 tgaacatcat caacacaagt ataacgatga taaggaaagc taactctgat ttgcgcttat
     3001 atttcgaccc catgatgatg cgtattcgac gattagatct cattgatctg gatgagtcct
     3061 tcttatatca agagatgaaa cagcacctct ctttattgat aaatcgggta atttttcgtt
     3121 atcgattgac tgatttattt tatggtgaga tagaaaaaaa gtatcctttt tcttaccaaa
     3181 tcgctagcga atgcatacaa gcattagaag ctatcttgca acataagatc tctaaagtag
     3241 agatcagcta cctaacgttg tatttggagt tagcatttcg atatcaaaaa gagcattttt
     3301 cctctaaaaa agtagccatt gttcatacga caggaaaagg aacagcatta atgatccaaa
     3361 gacaaatcaa aaggatattg gggagggata tacaaataac acatgtttca gaagaacaat
     3421 atgaaaaagt agatctaaac aaatatttcg ctgtttttac aacgattccg ttaaaaaatg
     3481 taaatgaaga gacaccggtg atccatttga ctaatctttt tgatgactct tggttaaggg
     3541 cacaatggca aaaagcaaaa aaaaagcgat tagccgggtt tcattctact gtttttttct
     3601 atcaaatatg gaaagaaaaa aagacgtatc aagaatattt aaacaaatta gtcgctcctt
     3661 tgatcagagg ctattatgtg gatgaagaat ttgaaaaaag gatcttgaaa agagaatatc
     3721 agcaaagcac atttgttaac aaagaatttg cttttcctca cacaaccaac cagctatctc
     3781 aacacattgt tttatcagta gggctaataa ctgatcaagg tttagcagaa acaggaggaa
     3841 atggagttat ttttttattt gccatacctg aagaaatgac ggctgaagtt gagaatgaat
     3901 tatttgagct atacgatatc ctatttgaac tcataaaaat gggaatagat gagtttattg
     3961 aattagcacc gacacaagaa tcatttttaa aactaatcaa aagtaaagga gagagacttc
     4021 atgaatgata tgtatctatt catagttttc gcagcttcag cttatgtgct gcaagcagtt
     4081 atcagttgga agcaactaaa gcatttcaat caaacatata gcgaactgcg aagaaaagga
     4141 aaagtagcga ttggtaaacg acctgggaaa atccgttcag gcacgattgt tatgctagct
     4201 atagacgatc atgatcaagt tttagaagtc agaaaaatgc aaggggttac atctttggca
     4261 aaatttcatt cattacctgt ctatgcgggt gaagatatcc attacatcga tcgttaccat
     4321 ccctcagttc gagcggaaga tcgtttgaca caagcagctt tagaaaatgc cagagaagta
     4381 tacttacgaa tagaggcagg aaatgaaaaa agtgaacaac cttcattgcc ttttattgct
     4441 atgctcaata attttcggat atggaaaagt acgatcaaag aaaaaaggag tgacaaacga
     4501 tggatatcat aacaaaattt gctgagggat ttatgaggct gttccaaaca ggtgcagaaa
     4561 cattcataag ctggatgaca ggaatcgtac cagttgtctt gatgctgatg gttgcaatga
     4621 atacgctgat tgctttttta ggtgaagacc gagtaacgaa agtagccaaa ttatcagcga
     4681 aaaatccgct tactcgttac cttgtgttac cgttcttatc tgcttttatg ttgggaaatc
     4741 ctatgtcatt tacaatggct cgtttccttc cagaatacta caaaccaagc tattatgctg
     4801 ctcaagctca attttgtcat acgagcaatg gtgtattccc ccatattaat cctggagaat
     4861 tgtttgtatg gatggggatt gctcaaggga tcgaaacctt agggctaaat tcgatggagc
     4921 tggctatccg atacttgctt gtgggactgc tgatgaactt tattggtggt tggataactg
     4981 attttactac tggatttgta tgcagacagc aaggaatcgt tcttagcaaa aaagtagagc
     5041 tttcagttga ttagagaaag gagagtttta aatgtatggt gatattaaga tcgtaagagg
     5101 aaacggcggg tatggaggtc ctttgattat taggccgaca aaagataaac acaagttcat
     5161 ctatgtcaca ggtggaggtg agaggccaat gatcgtagat aaaatagaag aactgacagg
     5221 tatggagtct atcaatgggt tcaaaacatc aatacctgat agtgaaatcg cgcttgcaat
     5281 cgttgactgt ggagggactc ttagatgtgg aatttatcct aaaaaaggga taccaaccat
     5341 caatatcgtt tccacaggaa aaagtggccc attagcacaa tatatcactg aagatattta
     5401 tgtttcagca gtagggttag aacaaataca gccactaatg gaaactgatg ataacaaaga
     5461 aacagtaaat actgcatctc cagataaaaa gtacaaatat gatacttcta aaaagataac
     5521 cgagcaaaaa gcagagcagc aaaactggat agcaaaaatt ggaatgggcg cgggaaaagt
     5581 agttgctaca ttcaatcaat cagcaagaga agcagtagat acgatgatcc ggacagtgat
     5641 cccttttatg gctttcgttt cccttttgat tggtgtgata caaggttctg ggatcggtaa
     5701 tgtatttgct aacattatgg cacctttagc aggaaatgca gttggattaa caatcattgg
     5761 ctttatttgt tcactgccat ttttatctcc tttgcttggt ccaggggcag ttattagcca
     5821 agtgattgga acattgatcg gtgtagagat tgggaaagga aatattcctc cccaattagc
     5881 attaccggca ctatttgcta tcaatactca aaatgcatgt gattttattc cagtcggtct
     5941 aggtttggaa gaagcaaaag ctgagacagt agaagtaggt gtaccatcag ttctttattc
     6001 aagatttctt aacggcatcc cacgagttat cattgcttgg gtagcaagtt ttggattata
     6061 tagctg
//
LOCUS       DANKHL010000167         6065 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000167 DANKHL010000000
VERSION     DANKHL010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.176"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            832..1566
                     /locus_tag="PW101_002289"
     CDS             832..1566
                     /locus_tag="PW101_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2514675.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="PW101_002290"
     CDS             1582..2751
                     /locus_tag="PW101_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2514676.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="PW101_002291"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="PW101_002291"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL2514677.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="PW101_002292"
     CDS             3841..4767
                     /locus_tag="PW101_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HDL2514678.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="PW101_002293"
     CDS             4786..6036
                     /locus_tag="PW101_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2514679.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2026 a          997 c         1245 g         1797 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacaa caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agtta
//
LOCUS       DANKHL010000168         6049 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000168 DANKHL010000000
VERSION     DANKHL010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.177"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..497)
                     /locus_tag="PW101_002294"
     CDS             complement(<1..497)
                     /locus_tag="PW101_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HDL2514680.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
     gene            complement(610..1176)
                     /locus_tag="PW101_002295"
                     /pseudo
     CDS             complement(610..1176)
                     /locus_tag="PW101_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(1510..1597)
                     /locus_tag="PW101_002296"
     tRNA            complement(1510..1597)
                     /locus_tag="PW101_002296"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1563..1565),aa:Ser,seq:cga)
     gene            complement(1742..2308)
                     /locus_tag="PW101_002297"
     CDS             complement(1742..2308)
                     /locus_tag="PW101_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2514681.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            2563..3894
                     /locus_tag="PW101_002298"
     CDS             2563..3894
                     /locus_tag="PW101_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HDL2514682.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3860..4210
                     /locus_tag="PW101_002299"
     CDS             3860..4210
                     /locus_tag="PW101_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514683.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(4302..4721)
                     /locus_tag="PW101_002300"
     CDS             complement(4302..4721)
                     /locus_tag="PW101_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDL2514684.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(4722..5066)
                     /locus_tag="PW101_002301"
     CDS             complement(4722..5066)
                     /locus_tag="PW101_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL2514685.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            5416..6012
                     /locus_tag="PW101_002302"
     CDS             5416..6012
                     /locus_tag="PW101_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2514686.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1683 a         1107 c         1037 g         2222 t
ORIGIN      
        1 taagtagcgt ccataaaaat agcgacatac ttatcattta aggctctgga tttaaaggca
       61 tttacttctt cagtcagaac tttagtcatg ttggacatgg tttgtggagt atagtgatga
      121 ccgtacattt tttcgatcaa atcagcaatt tcagacatcg taacaccttt ttcgaataaa
      181 tggataatag tggtttccaa tgtatcgttt gttcttttgt aggctggtaa agtttgttgt
      241 ttaaactcac cattacgatc tctaggtatt tccaatgtta attcaccata ttcggttttg
      301 attgatcgaa agtaagaacc gtttctcgaa ttacctgaat taaaaccagt gcgatcatat
      361 ttttcgtaat ctaaaaaagc cgttaattca gtccgtagga gtgtgtttat cgctttttct
      421 aagtgcgaac ggaataattc atttaaatcg cctttagtga ctagagtttg cacaatttct
      481 gtagtaaaat cattcatagg gaagtcctct tttctgtgaa ttggttgtcg ttaactttat
      541 tctacagaag ggacttcctt ttttgtatgg attttttcat ttacacaaaa tattttatac
      601 tctcgttcca tttgatagcg agcgataatt gttcagatta aataattgat aatcaaatgg
      661 cggattattt gtcaacacac cgacaggatt atcatataaa tgaagtccat ctttcatact
      721 ttcaattact atcgattttt ctttatcagc taataaccag tgtaaaggtg acaagggtag
      781 tttttcacta aaatcgatat tgactagatt gatagtagcc aataattctt tagcttcgtc
      841 tatggttgag cattgcccta gtatccatgg aataaactca aatggtgcaa cgttatcttt
      901 tccttcttgt aattctttat aatcagcatt gccaggaaaa ttcagtcctg ccatactcaa
      961 ccctttttca tttgtagcct cataatagag tggatagttg gataccccag ctgctattcc
     1021 tatcattgca taatgtttat ctaaattttt tacctttcta aaatcgaaac gataatttct
     1081 tggagtaata gtgaccactt cattataaga catctcataa tcaaaattcc ttccaaaata
     1141 atggtctttc gttgcataag tgattgcggt acacatcgtt tcccctccta aaacaaatcg
     1201 tttttacact tttatcttac atctagtatt ttttttatca aaaaaaggcc aaagaagatt
     1261 gatttactag catttataaa aaatagtact cagggggaat caaaaaatat tgtaggttaa
     1321 aagtacaaca ataaggggct gtgacaaaag tcttgtcaca gccccttatt ccgaacaaac
     1381 ggtgttcaaa agcttgcatc tgcggtaata agcccaatca aacgaaaaaa taggaaaagc
     1441 tatttttgtt tggccgttct tattacttga tgcaggacag cttcttcaca acctctaatc
     1501 gttgatataa ggaaggtaca ggatttgaac ctgcgcgccg gtattagccg gttcgccgga
     1561 tttcgagtcc ggtgcattac cactctgcca accttccgta agtactattt ttaaactttt
     1621 ttcacgaaat gtcaagccta tttctaatag ataaataaca gaaatcgctt tttttagcaa
     1681 aaaagtccat tgacagtttg cagccaacgg acttaccgat caaattcata ctgatagaac
     1741 actatctgat tatttcagga ttatctaatg ctaaaatcga gtcaattgtc cgtttgatca
     1801 gtgcaatgat ttgttcttct gttggatgca tgatgtttga tgacatcaat gacgctaagc
     1861 cagtcactgc tacccatgta ccagtatgca gagcatcgac atattcatcc gataattgat
     1921 tgtatctttc atcttcttta actatttcac ggaaataatt gtatgaaaaa tcatgcattt
     1981 tctttccgtc cccatgttca tcaatgtaca atgccatgta aagactatga tgcttttttg
     2041 caaagtagat atagttcaaa ccgagatcaa tgattttatc accagtacgt tcaataggga
     2101 aaacatcttc ttttagttgt tcaaagatag aatccagcaa cgtatttttt aaatcttcca
     2161 tgtttttgaa ttccagataa atcggctgtg tcgaaatccc catcttttta gcaatatttc
     2221 tcgcagtaaa tcctttaaac ccttcctttg caacaacatc ataagcagct tttagaattt
     2281 gatccttggt atagactttt cttctcatta aaactccccc acttctttac tgcagtatat
     2341 gttgttttat tgtaccctat ttttagcatt ttttgcaaac attttactca aaacgttata
     2401 ttttaaattt ttttcagtct tctttaactt ttttttgcaa aattaaaaaa atttggttaa
     2461 attcaatggg ttcccttaca tttttagttt tgacctaaat gatagttgtc caaatctttc
     2521 atttcaagtt atagttaaag aaaatgaaaa agaaggtgtg taatggaaaa cgaaactgac
     2581 gttatttata ttcatcctca aaaacgaatc gtttcacaga aaagaaagta tttttatctt
     2641 ggttttacag gtgttttttt tctttttatt ggcttgcttt ctaatactcc tactgataat
     2701 tggagtggat tgctaactat tcttacctcg ccaagcaatc ttttgacaga ttattttgct
     2761 ttaggtgttt tcggaagcgc attcataaat gtagggattt tgaccttact ttctgtgctt
     2821 ttagcttatc ggcacaaggt gattttaaac ggtcccttat ttgcttcgat tctaacagtt
     2881 accggtttct ctttttttgg caaaaacctt tataattcta tctcaatcat tttaggtgtg
     2941 tatctatacg ctgtatttgt caataaacct ttttctcagt acatcatgat cggactgttt
     3001 ggtagcgtat tatcaccggt cgttagttat atcacttttg gtatgcgttt tcctttactg
     3061 ataggtattt tattggggaa tttagctggc atagcaattg gtatacttct tccgccctta
     3121 gctgctcaaa ctttagtttt ccatcgtgga ttcacacttt ataatattgg attcacctct
     3181 gggttgattg cgatgacttt tactgctgtt ttgaggttat tcagctattc aatcgtcgaa
     3241 aatacattgg ttttcaatga atatcacttt cctctcattt ggattatttt tggctttttt
     3301 tcgctgacag tcggaattgg tttctattat aattcctttc ggctttcggg gattcgagaa
     3361 attttcgatt catctggaaa attgacaact gatttcattg ctaattctgg tattggtgct
     3421 actttgatca acatgggctt agttggtctg atgctgtctt cttatgtttt gctggttggc
     3481 ggtcagttaa atggacctgt catcggtgct attttatctg cggtgggttt cagtgctttt
     3541 ggttgtcatt taaaaaacag ttttccgata ctagtcggaa tatttattgc ttctttgttt
     3601 ggaacttttc atgagatcac cagtactggc atgcttgttg cagctgtgtt tgggacaggt
     3661 cttgcgccca taagcggttt ttacggcagt ttttacggtg taatagctgg tgtgctacat
     3721 attgctcttg tccataacgt cagcactttg cacggaggac taaatctata taacagtggt
     3781 ttttcaactg gtttcgttgc aggtatcctt gtaccgatac tagataactt taccgctgtc
     3841 agaaaggaaa aaaagacact tggaaaaaga attatcaaaa agaatcatcg atgagcttct
     3901 tacttattta tattcccatg aaataactga aatacgtatt ggagtagaat ttgctgagga
     3961 agggcttttc atagagatac agggtacggc agaaactcag ccagaagatt tagatgtact
     4021 tgctgatttg ctgaacaatc ctcgggaccc tagtatggaa atctactatc aagaactatt
     4081 aggtaatccc cgacatgatc gggaagatta tcatctgtta ggtagtttga ttgatgacgt
     4141 ggaaatcatg tatgcccagc cgattatcaa tattaagatc tttcgacgtg cttctcattc
     4201 ctattattaa ccctattttc tatttataaa agactgaatc aaaaaagcag ctcctgaaag
     4261 ttcgattaaa atctactttc aggagctgtt caagcttgac attattgatt ttccaatcct
     4321 tcatacactt catctgttac aggttctaaa aactctgaat taccagcagt gatcgccaaa
     4381 tgttcaaacc aactgtcttt tgtcgcacca tgccaatgtt taatcccttt gtgcgttacg
     4441 acgacatctc cagccattaa tcgtcttgct ggctttcctt cttcctgata ccagccttct
     4501 cctccggtca caagtaagat ttgatagccg tctgtatgga catgccagtg attgcgacaa
     4561 cctggttcaa aagttacatt acctacacca acagaaactt cgggatcatt cactaacata
     4621 ttcagatagc tttgcccggt aaatacttga gcgtaagctt cgtttttttc acctgctgga
     4681 aaaatcacgc cattgtgtat ttcttcgtgt tttgtcatca tttacgcctc tttcttatct
     4741 tcgctatttt cttctaaata gatttctttt gctaaactaa aagctgacca agcttttggc
     4801 cattctacat aaaaagctaa atgggtaatg atttctgcta tttcttcact agtaatgcca
     4861 ttttgcttgc caatcttcag atgagctggc aattgttcaa aatttcctcc tgaaattaat
     4921 gcagatatgg tgatgatact acgctgatga gcagataatt cttctctgga ccatacttgt
     4981 ccaaataaaa catcatcatt taattctgca aattttggtg caaaatctcc taattgatct
     5041 cgtcctgcgg tttgtttttc tgccatttct cttattctcc cttcacgact gttaggtgat
     5101 tcaactatgg ttctaaggca actttatttt ctattgtttc ttcgcatttc attttaaatg
     5161 tagtctttca gcctgtccaa tgcttagttt attcagtcga tatgcttttt aagcatagtt
     5221 gctaacgctt tttttatttt aaaaaaaaca agttattaaa taagtcttta gttggatgtt
     5281 tttttgagat caatctttta tacttatgat gaacttacaa aattgtttgt tttctgtcat
     5341 atcaaaaaat ggcgcagtta gttgcttttc tagtaaagta tagttaacaa agaacctcta
     5401 aggagttgat gaataatgaa tattgctgcc tctcgaaaac tgataaattt catatccatt
     5461 atcggattag gcctatcaat cgcgctgacg atttatttta ttaatttagg tgttttcaaa
     5521 gatttgaatg cgttaagagg attagtaggt gattccatca ttttaggacc gattattttt
     5581 gtcttcatcc aaatcctgca agtagttatt ccaatcattc ctggagggat cagtactgca
     5641 gctggtgtcc tgatatttgg cccttatgct ggatttatct ataactatat tggaatctgc
     5701 attggttcga ttatcatttt tctgctaggg cgtcgttatg gaaagccatt catactttcc
     5761 atgattagcg ataagacata taacaaatat attggctggc tggataatca aaaccgtttt
     5821 gagaagctgt ttgctctagc tatttttctt ccagttgctc ctgacgatgc gttatgtttg
     5881 atggctggat tgacaaacat gtcagtgaaa cgatatacac taatcatttt gattgctaaa
     5941 ccactgtcga ttttcttata tagtatggcc ttgatttatg gaggtcaata tctaagtggt
     6001 cttcttggat aatatttatt ttgtaaaatt agtcattgat tgacaatgt
//
LOCUS       DANKHL010000169         6026 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000169 DANKHL010000000
VERSION     DANKHL010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.178"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(32..907)
                     /locus_tag="PW101_002303"
     CDS             complement(32..907)
                     /locus_tag="PW101_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2514687.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            1055..1387
                     /locus_tag="PW101_002304"
     CDS             1055..1387
                     /locus_tag="PW101_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL2514688.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            1499..2707
                     /locus_tag="PW101_002305"
     CDS             1499..2707
                     /locus_tag="PW101_002305"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDL2514689.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            2861..3316
                     /locus_tag="PW101_002306"
     CDS             2861..3316
                     /locus_tag="PW101_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514690.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            3678..3953
                     /locus_tag="PW101_002307"
     CDS             3678..3953
                     /locus_tag="PW101_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514691.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            3966..>6026
                     /locus_tag="PW101_002308"
     CDS             3966..>6026
                     /locus_tag="PW101_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDL2514692.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKK"
BASE COUNT         1948 a         1021 c         1272 g         1785 t
ORIGIN      
        1 ctttcctcat agcctatctt tgtctttttt cttatagttt tttcagtaat cttgtctgtg
       61 gcttcaaata agtaaagcct tgcccttcca cttcatgcac attgatgatt gaaaggaaag
      121 ctttttcgtc aatctcgtgg acgatcctct tagcttcagt taattcacta gagctaacga
      181 ccatatagat gatttttttt gaattaccag agaatgcgcc ttctccagaa aggaaagtca
      241 cacctctttg cagaccggtc atcagtagtg gtgcaatctc ttcgcttttg ttggagacga
      301 caagtagacc ttttgcagaa tatccgccat ctaagatcga atcaatcaca cgactgaaaa
      361 catacgaaac aatcaacgta tacatcatac gtttcaagtc gatataggtt aatgaagcaa
      421 gtaagacgat gatatcaaat gccaataggg aacgccccat agtgatgccg taattttgtt
      481 ctaaaatccg agcgataata tcactgccgc ctgttgttcc acccatgcgg tagacgatcc
      541 cgcttcctac acctgcaact aaacccgcaa gtagagaaac gatcagtaga tcatgctgta
      601 aattgatttc tagtggaacc ctttgccaga tccataaaaa gaatgacaaa gaaagtgtac
      661 ctagaacagt ataggccaag gaacgtttac cgaggatctt tcctcctagt aaaatcaatg
      721 ggatgttgat taataaagtc gtataagctg gatcaatacc aaataaagct cttaagatca
      781 aggtgatccc tgagactccc ccttctgcta aatgattagc gatgttcaag taaacaagac
      841 caaatgcata aatacttgtt cctataatga taaaaagaac ttcttttact gtttttcgtt
      901 tgaccatatg aattccagct ttcaattatg tattttcaca gaatttccat ttagaaattc
      961 cttcctaaat gtagcatgga aattcgattt tctcaattgc tcttatctag gatttgcgtt
     1021 aaagttttgg taagatagtt ttggagggaa aaagatggat aaaaagcgtt ctttatacag
     1081 tatgcagcag gaagtggatg aatatatcca gcaattcaag gtggggtatt tctctccatt
     1141 agctcagatg gctcgattaa ctgaagaagt aggtgaattg gcaagagaag tcaaccacac
     1201 ttatggagaa aaaagcaaaa aggcttctga accagttaac tcggtagcag aagaacttgg
     1261 tgatgtttta ttcgttacga tgatcatggc taattcattg aatatcgact taactgaagt
     1321 ttttgaaaaa aacatggaaa aattcaatca aagagaccat aatcgctttg agcgtaagga
     1381 cgagtaaaca agatgaagtg gtcctggcct tgaagactct gaaacggact tgtcaaacag
     1441 tcaccatgca actgagtctt tatctcagga caagaaacac gcaggaaagg ttggaaaaat
     1501 gaaattaaaa caattacctc tggaatacca aaaggcaatt ccagttctga aaaaattaga
     1561 gaatgcagga tatgaagctt attttgtagg tggaagtgtt cgtgatatct tgctgaacca
     1621 gccaatccat gatgtggata tcgcaacaag cgcatttcca gaagaaatca aacagctttt
     1681 tccaaaaacg atagatattg ggatcgaaca tggaactgtg ttggtattag aaaatgatga
     1741 acaatatgag atcaccacat ttcggacgga atcaacttat caggactacc gtagaccaga
     1801 tcatgtcgaa ttcgttcgtt cgttggcaga agatttgaaa agaagagatt ttacgatcaa
     1861 tgcgtttgct ttgaaagaag atggagaaat catcgatttg ttcgatggtc tgcaagatct
     1921 agaagaacag atcttgcgag ctgtaggcaa tcctcatgag cggttccatg aagatgcctt
     1981 acgaatgatg cgcgggttgc gttttgtcag tcaactaggg ttcaaattag aggaagaaac
     2041 cttctacgcc atcaaagaaa accacgagct attaggtaaa atatctgtag aacgtataaa
     2101 tgtggaattc atcaagatgc tcttgggaaa tgaacgggcc gcgggaatcc atgcatttat
     2161 tgaaacagag tgctacattt gctgtcccgg tttgaaagaa gaaggcgaag cacttttacg
     2221 ttttgctgaa ttaccatccg tcccattgaa agaagagagc caagcatggg cattattgat
     2281 cagccaactg gatctaaaag aaaatgagat tcgttccttt ttgaaatcgt ggaagtgttc
     2341 taaccaaatg attaaagacg ttcaagcact tgtccaagga ctacgacaac gagaaactgg
     2401 agctttggaa ccattcgaat tgtacttctt aggcaaaaaa catgcactgc aagtagaaga
     2461 attgatgatc tattttgacg aaacacctca gatggatgcc gtaggagaat cctatgatcg
     2521 tttacctatc aagtcattaa gcgaattagc agtagatggt aaaatcttgc tagaagaaac
     2581 tggaaaaaaa cctggtaagt gggttagtga agctttgcaa ttagctgaaa aagccgtcgt
     2641 agaacaaaga ataaaaaatg ataaaaaaca ggttttggat tttttatcga aagaaaaatt
     2701 gatttaaaaa acactagact gtatctgctc agctttgttg gctacttaaa gtgggcagat
     2761 acgtcattag aagaaaaaac ataagatgtg accacttaca taaatttgtg ctaaactgag
     2821 gtagtacaag aataaattac atcataaagg agtcttttta atgagtaaag aaatgacagc
     2881 gctaaaattt tattttagaa atggagaaac ctggacgatc aaccgccgtc atatcggcga
     2941 tctttggatc aaacaaatca caacaagttt tggacgaatc aatggcagtg aatttgttga
     3001 aatccatccg tgtgctggat ttaaaatcga aatcttccat gaaggggacg cagttgcgac
     3061 acatgatatc aacctaggcg gactggaaat gggcatgttt aatcgcgcat tgaaatacga
     3121 agatatcgag cgaatggaaa tcctttaccg aaatggcaca cctgatcttg tgtacttccc
     3181 atatctagat aaaggaacag aaggattaga caatcaatat caatcaacaa aaatcagcga
     3241 aaaaacaggt aacttgtata tcgtgatcaa tccagatcaa cgtgtagaag atgtatacgg
     3301 ggaatttttc gaataaatcg atttttacgc aaaaatgagg ctgtgaaaga gatgtgtctt
     3361 attactactg ataggctgtc tcttccacac cgtttattca gaataaggga ctgtgacaag
     3421 atttttgtcg cagccccatt tttttgttgt gataaaagaa caagaaaggc aggaatggga
     3481 aagtaaacat ccttttgtca gaaactttta tctttcttaa gttttacttg atatctgata
     3541 ctatttggaa tagaatgagg cgttgaaaaa tttatggaaa tttctaacta gcaataaacg
     3601 atgaaacaat gaaatatttt tcatccaaaa agaactttgc tataatgatg gaagtttgtg
     3661 tataggagat ctggaaaatg agagagagtt ttgaacaaca aaaaaaactg ttgcatgatc
     3721 gctatggcgc cctttcgatg gatgaccgcc gtcaaatact ttgtaagttg cgtaaaagaa
     3781 atatattgat gtatcgtcag ttggaacgtc tgaaacatga tctgcttcgt ttggagtcaa
     3841 aacgtgtaca gtgtgaatta gaggggaacc aaacacaggt tgaagtagtc gaaaccaaaa
     3901 ttttaaagaa aaaagaacaa tttttaaaaa tgcttacaca aaataaaaaa taaaggggat
     3961 ggacgatgga atttgctgag ttagttattg tatttgcagt aacgatcacc ttatcgaaca
     4021 tagctagccg cattttgcca atgattccag ctccactgat ccaaattttc cttggagtgg
     4081 tattaggtct gacagagtgg ggacaatcaa tcgattttga acctgaattg ttcttagtta
     4141 tgattattgc accattgtta tttagagaag gagaaaaagc agatatttct tcgattctca
     4201 aaaatttcgg gacaattttg ttcttggcat ttggaggagt cattttgacc cttgtcgggg
     4261 taggtgcgac gttatcattc ctgttgccaa gtgtcccact tgctgcctgc tttgcttttg
     4321 gagcagcgtt aggtccgaca gatgcggtag cagtcagttc tctttctgga cgagtgaata
     4381 tccctaaaaa ggcgatgcat attttggaag gagaaggtct tttgaatgat gcatctggtg
     4441 ttactgcctt ccagtttgct cttggtgcgc tgatcaccgg atctttttca gcagtaaacg
     4501 cggggatgag tctagtagtt tctagtattg ggggagcttt gatcggcttt ttgcttgtct
     4561 ggttcaaaca aaaaataatt catttgatcg aaaaagcatc agcacaagat gtcacagggt
     4621 atctgctgat cgaacttttg cttccttttc tagcctatgt attggcagag gcatttgatg
     4681 tatctggaat cattgctgca gtggcagcag gtattctcca agcttctggt tttaggaaaa
     4741 tctctgtgtt tgatgcagaa ttgtcgagct tgtcccacag tacatggaca actattgctt
     4801 ttacactgaa tgcattagta tttatatttt taggaatcga attgacgcaa gtgttctcgc
     4861 cagtatgggg agacggttta tatccaaacg gtcttttatt agcgattatc gtattaatct
     4921 ctgtcatgct ttttgtgatc agattcattt ctatcagtct tttttatgta ttcaaagatg
     4981 gcagtaaaaa gttcaaaaaa cagttgaacg agatcttgat cttaacattt ggcggtgtaa
     5041 agggaacagt cagtttggct acgattttta ttttgccatt ttcaatcaat aatatgatgt
     5101 tttaccagcg gtctttgctt ttgtttctga cggcgggtgt catcttagtg actttggtca
     5161 tagggatcat tgtcttgcct atgctgacgg aaacagagga agcgaaaagc acggatttga
     5221 atgctttgat gattttagaa gaggtcgttg aagttttgcg aaaagaaata aaggaaattg
     5281 atcgtaatac aaaagaattt ttagccactg aagcagtgat agaaaattat caagaacgga
     5341 tacgtgatct gtacttggaa gatttaaccg atgatgaaaa gcaagaagta caagaaatac
     5401 aagcactgat tttgtcgatc gaacgggatg gattggatga aagttatcgt tcgggcaagc
     5461 tttcttctaa tggttatcgt ttttactccc gttttctttc tcgattcgaa cattctatca
     5521 caagccagat tttgtctttt atcggctttt ggttcattgt tgtccgtagg ttaatgcgaa
     5581 tcgttctcca tccgaaaatg ttttggcaaa gaagatatgc tgatcgtcag acatttatta
     5641 gtgatgaaga tatccaagaa atcagagaga catatcaaaa aaatactcag ttgattattg
     5701 aaagtttaga taacttgact gatatttatg atgaaacgat ggttcaattt tttatccagc
     5761 aacgcaaact tgaaggaata aaaatgaact cggggaattt gatttcttcg tggatgatcc
     5821 agcaagatag tctgtttacg aaaaaaatgc taagaggtta ttatcttgaa agaaaaatca
     5881 ttgatgaata cgaagttgct gaaaaaatca cgacgttttc tgcgaacagc tatcgcagga
     5941 atatcaattt gttagaatct tatacgatga acaaaccatc tgataacttt tctttccgat
     6001 ttgcgtttcg agcaaagtca aaaaaa
//
LOCUS       DANKHL010000170         6023 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000170 DANKHL010000000
VERSION     DANKHL010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.179"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(121..501)
                     /gene="yajC"
                     /locus_tag="PW101_002309"
     CDS             complement(121..501)
                     /gene="yajC"
                     /locus_tag="PW101_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL2514693.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(598..1743)
                     /gene="tgt"
                     /locus_tag="PW101_002310"
     CDS             complement(598..1743)
                     /gene="tgt"
                     /locus_tag="PW101_002310"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDL2514694.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1961..2659
                     /locus_tag="PW101_002311"
     CDS             1961..2659
                     /locus_tag="PW101_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL2514695.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2827..5466)
                     /locus_tag="PW101_002312"
     CDS             complement(2827..5466)
                     /locus_tag="PW101_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2514696.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
BASE COUNT         1694 a         1323 c         1105 g         1901 t
ORIGIN      
        1 acacacccga ttattcaata ttgagcaata tatcccatgc tcttatctct ttcttttttg
       61 aataaaaaat gaggtaggac atctttctgc cctacctcgt ctttaaataa gctttgatac
      121 ttatttttct tccttctctt ctgtctcttc cagcgtttct gttgtttcag tttttacttc
      181 aactgtctca gctggttctt ctgttgtttc aacagtagtc gttgcatcat ttgttacgac
      241 agtccctggt ttgaccgttt tgattgctgc acgatcgtac tcaaggaaga tcccttcgca
      301 gtctaaaaca actgtccgtt tgtcattgtc gatttctgaa acaactccat gtaatccgcc
      361 gatcgttacg actgaatcac ctggtttcat gctatctaac aagttttgac gttcttgctg
      421 ttgtttcttt tgcgaacgtg acataaaaaa ccacattgct aatagcgcga taaacatcac
      481 aagcattggt aatgttccca tttttttcac ctcactaatt ctgtatctac aaaaaacact
      541 ttagcagaaa aatgctgata acactagttt tttacttaat ttttaaaaga ttttccttta
      601 gaagcttttt gcgttttctt tattgaaccc gtattcttca aaaaaggctt ctctaaattc
      661 aagcaaatta tcatccatga ttgcttgacg tacttgtttc attagattca acaagaagta
      721 taaattgtgg taagaagtca aacgaatccc aaatgtttca tcacatttga tcaaatggcg
      781 gatataggca cgtgtatagt ttttacatgt ataacaatca catttctcat caattggacg
      841 gaaatcatgg gcatatttcg catttttcac tactagacgg ccttgagaag tcatacatgt
      901 gccatttcga gcgattcgag taggcaagac acagtcaaac atgtctactc cacggataac
      961 accatcgatc aatgaatcag cagcaccgac acccatcaag taacgtggtt tgttttcggg
     1021 gatcaacggt gtagtgaatt ctaatacacg attcatttct gatttaggtt ccccgacaga
     1081 taacccgccg attgagtaac cagggaaatc catcccgact agatctttcg cgctttgacg
     1141 acgaagatct tcaaaccctg ctccttggat gattccaaat aatccttgac ggtctggatt
     1201 agcatgagct ttcaatcctc gttctgccca tcgagacgta cgttctactg attttttcac
     1261 gtagtcgtaa ctttcatcga atggtggaca ttcgtcaaaa ctcatcatga tatcagagcc
     1321 caatttattt tggatgttga ttgctttttc tggtgataaa aacatacgcg aaccgttcaa
     1381 gtgatttttg aaatgcacgc cttcttcttc gatattccgc atatcactta atgaaaaaac
     1441 ttgaaagccg cctgaatctg ttaaaatcgg ttgatcccag ttcataaatt tatgcagtcc
     1501 gccagcttct tctactaagt cttcaccagg gcgtagccat aaatgatatg tattacttaa
     1561 aataacgcca gcacccattt ctttcaactc ttctggggac attgttttta ctgtagctaa
     1621 tgttcctact ggcatgaaca ttggtgtggg gaaagttccg tgtggcgtga tcaattcccc
     1681 taaacgcgcg ccagtatgtt tttctttctt gattaaacga tagcgaatgg caggttgcgt
     1741 cattcatttc cctactttct atataaaata tcttgacaac tcccaccatc ataatcgttt
     1801 caagtgcttt ttgcaagttc cctcggcttc cttctacttt atttaatatt ctttttttta
     1861 tccttttttt atgaataaca aaaaaaagaa aaacattatg acagaaatat tttttgatac
     1921 atggtatatt ggtgttatta aagaaaagga gggaaaaatc atgcaatctt ctgcagaatt
     1981 aaaacgccaa gcaaaagaga gtctaaaagg acgttgggga caagctgttt tactaaatct
     2041 aatccctaca ttgattggta ttgctactat tcttcttatt atgattccta ttatcttgtt
     2101 tgccaccacc atatatgatg gatcagcgag cgtctcagaa gcgacaactt caggaggcgg
     2161 tagttcagga ggcggtggcg gaattatttc cagcatcatt agcgctttgt tcatgagcgg
     2221 tatttcttgg acttatcttg atattttacg tggacaaaaa gatcagatcg aaccattcaa
     2281 agatgcattt cgtggattca aaggtttgtt tatcggcgga gtgatcagct tagctgtgct
     2341 gatctcgatc tttacaacat tatggggttt gctattagtt attcctggaa tcattaaaag
     2401 ctattcttat tctcaaagct attttattta ctacgatatc gtccaagaaa caggacaaac
     2461 acctaaagta ctggatacga tcactgcaag tcgaaaattg atggatggtt ataaaggaca
     2521 acttttctgg ttagatcttt catttatcgg ctggcatatc ttagcattag caactgctgg
     2581 tattggttat ctatggctca gcccatacat ttctgcaaca aaagcagcat tttatgaaca
     2641 attgccaaaa gatatttaaa agagagtgtg acttgtgacc gagggggtgg caggagaaca
     2701 ccgtttatcg agaggcagta aaagaaacat tcagcccatt agcaaaaagg aggtgtgaca
     2761 acattcatgt cacacctcct ttttgtatag ttccagtaaa aagaaaatca acttagaaac
     2821 agttaatcat tcctcttctt ctgcttccgg taatcgatgg aacttgatca gttcattcac
     2881 cgcgattggt acgatagata aaatccctac caagaaccag tgattcaaac tgatgtgtac
     2941 taggccgaat agttcttgcg taaatggcag caaagcaaca gccgttgtaa tagctaatgc
     3001 cagtaacgcc atttctacca aagctttatt ggtcgctaaa ttgactttga agatcgattg
     3061 gctgctgcgt acattgaaca catgcagaat cgatgaataa gctaagatca agaatgcaac
     3121 agtttgaccg acatgctgac ttgcgtctac ccatgggctg atagtatcga cataactacc
     3181 taaataataa ccaaacaagg ttacgatggt gaagacccct gcatttatgg caattttttg
     3241 caataaacct cgagcaaaga tcccttcatt tttcggaatg ggtggctgat ccatgatgcc
     3301 gtgttccgct ggctctcttc ctaaagcaaa tcctggaagt ccatcagcaa ctacattgac
     3361 aaacagtagt tgcacagctg taaatggtgc tccccatcct aacagcatcg caatcaaaac
     3421 aataaagatc tccgaaatat tacagcttag caaaaagttg atagctttac gaatattctg
     3481 ataaaccgaa cgtccttgac tgacagcgtc tacgatcgtc gcaaagttgt catccgtcaa
     3541 aatcatatct gcggcacttt gcgccacatc tgttcctgtg atccccatgg cacacccaac
     3601 gttacttgct ttcaacgctg gtgcatcatt cacaccatcc ccagtcatcg ctacgactgc
     3661 acccgtacgt tgccaagatt tgacgatccg gatcttgtct tctggcgtta cccgagcata
     3721 gacagagaga gctttgacac gcgcatctaa ttcttcgtct gataattgat gcagttctga
     3781 tcctgaaagt gcttctgacg gatcattcaa gatacctaat tctttcgcta tggcactggc
     3841 agtcaccaca tgatctccag tgatcatgac tgttttgatg ccagcctttt tcgctcgctt
     3901 gattgctcct ttactttctg gacgtggtgg atcgatcatt ccgatcagac caatcaaacg
     3961 taagtctttt tccagtgctt cagaagaaat atcttgcggt tcttcgtcat aagtagcata
     4021 accaacagca atcactctca gtgcccgttt accaaagcga tcattaacga tcgccgcttg
     4081 ttctacatcg cctgaactaa agcgcggagc aagaacatcg aatgctcctt tggtgattga
     4141 gatgtatttt tcgcccattt ggtgaactgt tgtcatcatt tttcgttcag aatcaaaagg
     4201 aagctcgttt actcgtggat atttttcttc taattctgcc tttgtatgat aattctcttc
     4261 aacagccctt acgatggcta cttctgttgg attacctttg ataacaaggt cgtcatcttc
     4321 ttgctcgacc gtcacatctg tacatagtgc agccattttc aatatttcca tcgcgctgtt
     4381 cgtcattgaa tcttcgatat tcgttacttc atcttctttg ctccataccc ggcggacgcg
     4441 catttcattt tgtgtcagcg ttcctgtttt gtctgagcag ataacattag cggttcctag
     4501 cgtttcaact gctggcaact gtcggatgat cgcatgtttc ctcgccatct tctgcacacc
     4561 gtatgcaagt gtcaacgtaa caataacagt caaggtttcc ggtacagctg caacagccaa
     4621 ggaaatcgaa gtcatgaaca tctcaaggag tggttctcct tgcagttcac caatcacaaa
     4681 tactaaagcc gcagccgcta aagcgatcat actgattcgt ttgcctagct gattcaatcg
     4741 gacctgtaaa ggtgtttttt gcatagtatt ttcgttcaaa agatccgcga tcttacccat
     4801 ttctgtagcc atgccaatgg cagtgacgat cgctttccca cgacctgcag caaccgtaca
     4861 tcctttaaat accatattaa gttggtcacc taatgactca tcttcttcag caataaatgc
     4921 tgcatctttt tcaactgctt cactttctcc agtcaacgcc gattcttcta cacgtaattg
     4981 tgatgcttcg atcaaacggg catcggcaga aatagcatca ccggactcta aaacaagaat
     5041 atcacccact accagttgct ctgactcaac agtggtcact ttgccgtctc gtatgacagt
     5101 tgccgtttgt ttattcatat cttgcaagga ttccagtgct ttttctgcgt ttccttcttg
     5161 gacaatcgcc aataccgagt tgatcacaac gatagcgatg atcagcagtc cttcaaataa
     5221 atccccatgt tctgtagcaa atgcggtata aaaggaaatc accgctgcta caagaaggat
     5281 gatcgttgta aaatcggaca agcttcgaaa gaattttttg atcatcgatt ctttcggcgc
     5341 ctcatcgaat ttattcaatc cctgcttttt tcgccgttca tccacttctt gtgaagacaa
     5401 cccagtatct ggatctgctt ctgttacctt ctttacttct tctattgtct gcttgtattc
     5461 ttccatccta ttcctccttt aaaatcttcg tacaaaaaaa ggtacttatt ttactataac
     5521 atcctttttt tatttacttc aaataaaggg cttatcgatt atttgttaag aaagcgtaaa
     5581 atttaaagtg cttatatttg caaacgctac caaaggatgc tattatatag atgtaaggaa
     5641 ggaggatacc atcaaataga cagaagaatt tctcgataga acagtgtaag caaacaaagt
     5701 tgttagaagt acaggtttca cccagacatg cgtgtagagt acaaacctat aaatagtgaa
     5761 tgcgtatgga tgtttggatc acgaagtatc cgaaacaaga agtaatgcga tacgtaacaa
     5821 ttgaataacg taaacgtctg aaaattgaat aggaacaaga gatgtttcaa atgttgaaga
     5881 tgtaacgaaa acaaaatttt tgtttaacat gaagaaattc aacaccgtgc tttgcatgta
     5941 tgatggaaag ttacaatcat ttctgtttat ttgaatcctt ccgaggtaca agaaagccga
     6001 gcttatcgag agctcggctt ttt
//
LOCUS       DANKHL010000171         6020 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000171 DANKHL010000000
VERSION     DANKHL010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.180"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            99..1049
                     /gene="yidC"
                     /locus_tag="PW101_002313"
     CDS             99..1049
                     /gene="yidC"
                     /locus_tag="PW101_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL2514697.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(1228..2202)
                     /gene="lacD"
                     /locus_tag="PW101_002314"
     CDS             complement(1228..2202)
                     /gene="lacD"
                     /locus_tag="PW101_002314"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL2514698.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKIPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(2199..2657)
                     /locus_tag="PW101_002315"
     CDS             complement(2199..2657)
                     /locus_tag="PW101_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2514699.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIALDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIATLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            complement(2654..4117)
                     /locus_tag="PW101_002316"
     CDS             complement(2654..4117)
                     /locus_tag="PW101_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2514700.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGIKIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAMSGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMVKAVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLAQGN
                     TTFMAGVSAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEKGRAGDLS"
     gene            complement(4099..5049)
                     /gene="pfkB"
                     /locus_tag="PW101_002317"
     CDS             complement(4099..5049)
                     /gene="pfkB"
                     /locus_tag="PW101_002317"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL2514701.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPKIPQTAVHNFLNQIRALQAEDYLCVSGSLPRGLSPKVLIEISRICQEKGI
                     HLIIDSSDQEAMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVELGAENV
                     LLSLGEKGAILFTKDRVFRGNSPKGKVVNTACAGDTMLGAFLAGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFETWENIN"
     gene            5192..5941
                     /locus_tag="PW101_002318"
     CDS             5192..5941
                     /locus_tag="PW101_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2514702.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMGEN"
BASE COUNT         1828 a         1309 c         1040 g         1843 t
ORIGIN      
        1 cgaatattta tatgaaattt ctatgaagtt gattgaattc catgcgcttt tttagtatag
       61 ttaagtttgt gtaaaaatta tagaagagga agtattctat gaataaatgg aaaaactggt
      121 tattgggatc cggacttgtc agtatcctgc tctttttatc tggctgtgtg cggatggatt
      181 caaacggaaa ccctgacact tcaggaatcg tttatcgagt actcgtccat cctatgggcc
      241 aagcgattac atatctagtt gagaatttca attggtctta cggctgggca gttattgcaa
      301 tgactgtgat cgttcgtatc atcatcttgc cactaggtat cagtcaatcg aaaaaaacaa
      361 tgatccaatc tgaaaaaatg caagcgttaa aaccacaagt tgaagcagca cagcaaaagc
      421 taaaagctgc aacaacacgt gaagaacaaa tggcagcaca agcagaaatg cagcaagtat
      481 atcgcgaaaa tggattgagt atgaccggcg gaatcggctg tctgcctcta ttgatccaaa
      541 tgccgatctt ctctgccttg tattttacgg cacgctacac agaaggtatc cgggaatcat
      601 ctttttacgg catcgatcta ggctcaccaa gtatggtgct tgtggccatc gcaggggtcg
      661 cttatctgct tcaaggatat atttcaacaa tcggtattcc tgaagaacag aaaaagacga
      721 tgcgtacgat gctgatcgtc tctcctgcta tgatcgtctt catgtcaatc agtgcgccag
      781 ccggtgttac actttactgg gtagtcggtg gtgtgttcag ctgtctgcaa acattcatca
      841 caaacgttat gatgaaacca cgtttgaaag cacaagtggc agaagagttg aaacaaaacc
      901 cgccaaaaca agtcgtcact ccaagaaaag acgtgacaga atcagcaaag ccagctaaca
      961 acaaacaagc aacaaaacaa atcaaacaac caaaaaacag tacaaacaac aaaggccgca
     1021 acgcaggaaa gcagcagaag cggaagtaga tagtaaataa gagtgtaata aaaattcatt
     1081 aacagtttta gcaattaaaa atatcgacca agatttttga aaaagaaatc tggtcgatat
     1141 ttttatttaa gtgaatgttt aatttgaatc atcgattgat cgctatctaa ttgcagcagc
     1201 atctttttat gaagaaacaa ctttttctta tctatcaacg tagacaggtg ttgctgtttc
     1261 ttctaatagt gtacttaatt ctttaatatt ttgttttcct tcttctaaca tccaactact
     1321 cgctttttct tcgccttcgg taacaaaagg tgccactccc ttcgcccatg ttgctcgccc
     1381 acataagaca ccattgaatg tcgaacctga ttgttttgca aaacgtaatg tatcttgaaa
     1441 tagcgtagca ttgactcccg cacttaaaaa aataaacggt tgttcagtca ctttcgattg
     1501 ttcatagaaa tacgcggatg cctctgcttg actataacaa gtctcttgcc catatccttc
     1561 cacaaatctc atattcactg gcacttctac tttcaataca tccacttgat aacgttcttt
     1621 tgaaaattct cgcatggctt caataacttt atgtggtttt acttttgcat aggcaagaga
     1681 agacggatcg ccaatagttg catcataaga aagaatctct aaaaagaaag gtatgccttc
     1741 tgcttcgcat tctgaaccaa tccgttctat aactatttgt ttttgcacat taatctctgc
     1801 tggttcatca acgtcatagt acaataaaaa tttacaagca tccgcaccag cttcttttaa
     1861 ccttttcacc gaccaaatat tcaataaatc aggtaatctt cccggtattt tcgtatcata
     1921 gccggttttt tcataagcta gcagtaagcc acattctttc tctcgaagcg ctgctgcagg
     1981 caggccgtat tctggatcaa gtaaaatagc tgaggcatag ggagtcaggt attgagaaac
     2041 catctgttta aattggatga tgtcttctga tgtagcttct ttatattggt tgatcatttt
     2101 cttcaaagca ccacgctggt cgattgctaa tgcgctaata attccagctt catccgtcaa
     2161 tcgcttcaaa cgttcctgtt tttctatact catcttcttc atagatcttc acctatttct
     2221 ttagttaata ttgtggctat ttctgttttt gattttgctt ttctcaactg ttctgtaacc
     2281 tcttttcgca tcaatgatcg tgcaacgaga gacaataatt tcaaatgagt cgttcctgct
     2341 tctgaatctg gaataaataa cgcaaaaata aaatcaactg actgtctatc taaactgttc
     2401 cattcaattc ctttgttcag tgtggcgata atcatactag gttcgttgat tgtttgagac
     2461 ttcgcatgag ggatagcaaa acctgacatt attcctgtag tcccttcttt ttctctttct
     2521 agaagttttt gatatacttc tttttcgtca agagcgatcc ctaaacagaa tgtttgcttt
     2581 gctaaaaact taaatgcttc ttcttgacta tccaatggct gttgtaaaaa aatgttctct
     2641 tcatgaatga ttgtcatgat aagtctcctg ctcttccttt ttcgataact gtacgatttg
     2701 ctgctttatt atccatcttc ttgtctttat cagttactct tttcttttgg attaatccta
     2761 gtatcactcc accaacgatc gaaccaataa gaatcgctgt aacccattgg atttttcccg
     2821 tcactacagg taacactaaa aaaccaccat gtggtgctgg aacttggacg ccaaacaaat
     2881 aagttaagac agcagcaatt gatgagccta acatcatcgt gggtaacact cggatcgggt
     2941 ctttcgcagc aaacggaata gctccttccg taatatgagt tgatcctaaa ataaaattaa
     3001 ccaaacctgc attacgatca ttggtatcaa aatatttacc aaacaataaa gtagcaaaac
     3061 cagtaatcaa cggtggtgca atacaagctg ctgacacacc agccataaaa gtcgtgtttc
     3121 cttgtgccaa aagagctgtt cctgttacat atgccgcctt attaaccggt cctcccatat
     3181 caaacgcaca catacagcca acaatcacgc caagaacaag tggattcgaa tgttcaaatc
     3241 cagctaaaaa gctcatcatt ccttcattta tagccttcat tggacctgcc agcaaagaca
     3301 tcacgatgcc aataaatgct acaccaataa ctggatataa gaagattgcc ttcaagccat
     3361 ctagttgttt aggtaaaatt gtcagtagct tttgcaaaaa taagataaaa taacctgcta
     3421 agaacccagc taaaatacct cctaaaaatc cagtaccgcc attcatggct acgattccac
     3481 caacaaatcc aacgactaac cctggacgtt tcgcgatccc ttctgcaata taagctgcta
     3541 aaactggaac catcattccc attgctgcat cgccaactgc ttttaccatt gccgctgtag
     3601 cattgtattg tgcatgatct ggatcaaaag aatagattcc ccaaacggca aatgaaatag
     3661 caatcaatac tccgccagct accacgaaag gcaacatatg agagacaccg ttcattaaat
     3721 ttttatagat gctacgacca aaattagcac cactcatcgc ttctggttct aaagtatcag
     3781 cagcttcatt atctgctaag acagtacctt ttcctgccaa tgcttcgtca atcagccgtt
     3841 gtgcatcctt gattcctgta ctgacagaaa catcaatgac tcgttttccc gcaaatcttt
     3901 ctgaatggac atctttatct gcggcaataa tgacagcatc tgcctgtttg atttcttcag
     3961 ccgttaattc attttcgatc ccaatttgtc catgggtctc cactttgatc ttgatgcctt
     4021 ttttcctagc tgcttgttct aatgcctctt gcgccatgta agtatgcgca attcctgtcg
     4081 gacaaccggt tgctgcaatt agttgatatt ttcccatgtt tcaaacctct cttccttgat
     4141 tttgatttgt ttacttaatt cctccacatc caaaaaatca gtcagtcctt tacgaaaagc
     4201 ggttgagctg cctgctgcta tgcttttacg taacgtttca tcaagcggtc gtcgattcat
     4261 atatcctgct aaaaaagcgc ctaacatggt atcacctgca catgctgtat tgacaacttt
     4321 tccttttggc gagtttcctc gaaacaccct gtctttcgta aaaagaatcg ctcctttctc
     4381 accgagggat aacaaaacat tttctgcacc taactctact agtcgttgtc cataaacaaa
     4441 gtaatcttca tctgtaacca tttttcgtcc gaaccaagaa gaaagttctt cctcgttcgg
     4501 tttcagcaaa aacggtcgat agggtaaaca atccatggct tcttgatcac tactatcaat
     4561 aatcaaatga atcccctttt cctgacaaat ccgactgatt tctattaaaa cctttggaga
     4621 cagccctctt ggtaaactcc cagaaacaca aagataatct tctgcttgta atgctcttat
     4681 ttgatttaaa aaattatgga cagctgtctg cggtatttta ggtccttgat tcactaattt
     4741 gtattcttct cccgtttgat tgacttgtgt aaatacattg atacgcgtca taccaggcac
     4801 ttcaataaac tctgtttgga tttgtttctg ctgtaaatag tcttctatat actttcctgt
     4861 aaacccagca gaaaaaccta atgccgtact tgggaaatct agcattttta atactagtga
     4921 cacattcacc ccttttccgt ttgcctgaat gtcatcctct tttgtgcggt ttaccataaa
     4981 aggatgcatc tcctctgttt caataaatag atcaattgcc aaattcatcg tgcatgtgta
     5041 gatagacata accaacgacc tccttgactc tattatgaaa tacgcgaaag agaaaatgtt
     5101 ttcttaatcg tcccaacaca aagaaaacct ttacatttat aaactcgtcg tgctattttt
     5161 taactaactt ttggtgaaag aaggaaagaa tatggatcac aaattaagtg aaacagaaca
     5221 gtacctctgg aactttatcg aacaccacat cctagagatc cctaactact ccatcgtgaa
     5281 actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga aaaagaaagg
     5341 ctatgaagga tttacttcgt ttaagcacca tctaaaagaa ggagaaaaca agaacatcaa
     5401 tttttctttc ttagataaag tagacaaagg tatccgtcga gccattctaa aaaatgaaca
     5461 agaagtggta cgtacgatca atatgttgga aatcggaaat attgaggatg caatacaaag
     5521 aatcaaattt gctgaccggg tttttgtctt tgcacgagga ttttcagaaa tgattggtca
     5581 agagatgctc gtaaaacttc aattaacagg gaaaaattgt caaatgcata ctgatccaga
     5641 aatcatcaaa agcatcagtc ggaaactaac taagaaagac agcgttattt ttgtttcttt
     5701 gaacggcgag acaaaagaac tcgtcaccgc cgcaaagaat tgctatgatg cagagattgg
     5761 ttcgattcta gttacagcca atcattcctc tacactgaaa gactattgcg agatcaatct
     5821 cgtaggtttc aaatcagaag gttcttattt ccctgattat gaagtacggt cacgtttgcc
     5881 tctccagatt atagcgagaa tcctgcttga cgcctatgta ttgcgtatgg gggagaattg
     5941 aaaagcagtt tattatggtt ggttacagtt taaaacatca cattttttat tctttatatt
     6001 gtatggactc acggaaactt
//
LOCUS       DANKHL010000172         5832 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000172 DANKHL010000000
VERSION     DANKHL010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.181"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(58..516)
                     /locus_tag="PW101_002319"
     CDS             complement(58..516)
                     /locus_tag="PW101_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2514703.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(529..1860)
                     /locus_tag="PW101_002320"
     CDS             complement(529..1860)
                     /locus_tag="PW101_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HDL2514704.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1871..3313)
                     /locus_tag="PW101_002321"
     CDS             complement(1871..3313)
                     /locus_tag="PW101_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2514705.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3300..4064)
                     /locus_tag="PW101_002322"
     CDS             complement(3300..4064)
                     /locus_tag="PW101_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HDL2514706.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4168..5043)
                     /locus_tag="PW101_002323"
     CDS             complement(4168..5043)
                     /locus_tag="PW101_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2514707.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5298..5382)
                     /locus_tag="PW101_002324"
     tRNA            complement(5298..5382)
                     /locus_tag="PW101_002324"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5346..5348),aa:Leu,seq:caa)
     gene            complement(5400..5471)
                     /locus_tag="PW101_002325"
     tRNA            complement(5400..5471)
                     /locus_tag="PW101_002325"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5437..5439),aa:Gln,seq:ttg)
     gene            complement(5481..5553)
                     /locus_tag="PW101_002326"
     tRNA            complement(5481..5553)
                     /locus_tag="PW101_002326"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5518..5520),aa:His,seq:gtg)
     gene            complement(5615..5699)
                     /locus_tag="PW101_002327"
     tRNA            complement(5615..5699)
                     /locus_tag="PW101_002327"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5663..5665),aa:Leu,seq:caa)
     gene            complement(5720..5790)
                     /locus_tag="PW101_002328"
     tRNA            complement(5720..5790)
                     /locus_tag="PW101_002328"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5756..5758),aa:Cys,seq:gca)
BASE COUNT         1771 a         1247 c          892 g         1922 t
ORIGIN      
        1 aaaaattttg taaattcacc cataaatatc accctattta acttttttca tcgtacacta
       61 aaattcaaaa caactatctt taatctttac ttcttggttt tgttttaata aatttccttc
      121 aaaaatgcct ggagaaacta caatgatcac cgtatctttt tgtgcttgct ccaacttctt
      181 caaatctata atagctaaca gtgtcccttc ttttacctcc tggttacttt caacccgtat
      241 ctgaaacgga cttccttcga gttcaactgt atctaaaccc atatggatca gtatttttct
      301 gccagattca ctttcgatcg ttatggcatg tcgggtagga aatatattat cgattattcc
      361 tttaactggc gcatggatat atccatcatg attcgttata gcgtatccaa ctcctatcat
      421 ttttgaagaa aagacctcgt cttttactgc tgttaatgga ataatttgac cactagctac
      481 agcttttaga atgtttcttg ttttaaagaa atgcactttt tatcacctct actttaatgc
      541 tggccaatat tttttgttta ctttgatcat ttcatctagt attttttttg cgactctggc
      601 atttggtacg gtttgattca atgtaaatgc ttgtaaagct ttttgataag aaccttcaaa
      661 gtaagcatct actagtaact tctctactgc aacttgtgcc tccatcaatc ctttatgaaa
      721 atcactgatt ggttttctta gtgtaattgg ctccacacca gttgcaccaa catatgccgg
      781 tatttcaaca actgcatctg accgtagatt cggaatcgcc ccttgattag gaacgattag
      841 cataaagcga cgacgatcat cattcaacaa ggaaatagct atatcgacga tatattgtcc
      901 atgctcccca aaattaaagt tcaatacttc ttccacaata ccgcggcgta ttttatcagc
      961 catttctttg gtatttttct ctcgtccatc catgaccata ttagcacgtg tatactcttt
     1021 gtcagtatgc tcaacgacca tatcaggata taagtaatac tctaagtaat tattcgggat
     1081 attacttgga aaatttttcg tgatgactga catcatttga aaggtttttt gccaagtttt
     1141 atctcctata ttaaaatcgg cgacttgcat ttcttttgtt attagttttt caataatctc
     1201 tggcataatt tctcgttgca attctttatc ataaatcgat gtataccagc caaagtggtt
     1261 taaaccataa tacgtaacga tccaattctt acgatcataa ccgtaattca ctgcaatcgt
     1321 ttcttcaatt gaaatagtca tatcacaggc attaatcatt tttacattcg gatatttacg
     1381 acgaacagct tcagaaacga tcgactctgg atttgtataa ttcaaaatcc atgctttggg
     1441 ggcatagtct tgtatatgat cgacaatttc caataaacct ttgattgagc gcatcccata
     1501 agcaaatcct ccgagtccac acgtttcttg tcctactaat ccatgtttaa gtggaatctt
     1561 ttcgtccttt tctcgcatct ccaatcctcc aacacgaatt tgtgagaaaa caaaatcgca
     1621 tcctgtaaaa gcaagctttg gatcatccgt tgcacgaatg cgaatatttg aaaaaccttt
     1681 tcttttcagc ataaatttaa caatcaagta catatccata tttctttctt catcaatatc
     1741 atataaacgt atttcagata atggcaaccg tttttcattg ttcaaaatgg cttgaacgat
     1801 tcctggtgta tacgtactcc cgcctccagc tatagtgata atctgttttt tcatcatcat
     1861 cttttccttt ctattcttct aataatttat tgaattctgc cataacataa gtcacttcta
     1921 agccgatgat aatttgataa tttttttcag ttggtttaat gatcccgctt atccctaatt
     1981 gcttgattcg ctgttcctcg accatggtgg gatccactac ctctacccgt aggcgtgtat
     2041 tacaactata gacatccagg atattggctt ttcctccaaa cgcttgtagc atttttgttg
     2101 ccatatattc ataattttta cttgctaacc ctaactctgt ttcttctaag gttacttcat
     2161 tgccaaactc tgccttagct tcacgtccta atgtctgaat gtttctctta ttgatcaaat
     2221 aataaaaagt aacgtaatac aaggcaaaaa agaaaattcc gattggaaga atcaaaatag
     2281 cattggtagc gattcgaaaa ttgatgagat aatcaacaat acatgctccg aatgtaaaac
     2341 caattcgtat attgaataag taacagacaa tccctgctaa accggcaaaa agtgcatgga
     2401 tcacgtacaa cataggagaa agaaacatga aactaaattc aacaggctca gtgatatttg
     2461 caataaatga agttcctgca ccgcttccca tgattccttt gaccatctct ttattttctt
     2521 tataagctgc tttatagata gctagacata tagctggtat accaaacatc attactacaa
     2581 aaaaaactga taaaaacagt ccagcctgag gatctccgtt gataaagcgt gtcatctctc
     2641 ctctgaaaac ttccttgcta ggagatgtat agcttcctaa atcgtaataa atatacgtat
     2701 tcaacacatg gtgcagtcca aaggggatca gcaaacgatt caaaaacata aatatcccca
     2761 cacctaatgc tcccatatca acgagcagtt ttgcaaaatt atcaatccca atttggatga
     2821 gcggccagat aaagcccaaa aaaacagctg cgattgtttg tagaatcatc accatagtca
     2881 atgggaattt ttcgcccgca aaataagaaa aaactactgg taatttttgt tctttaaagc
     2941 gattatacgt ccatgccgct actaaaccgg aaataattcc accaaaaatc cccatattca
     3001 cttctgggtc cataatttct aagccttttt tcaaggtagc aatcgctaaa aaaccagtaa
     3061 gtgcaggtat tcctttgtcc ttatttttga caaaaccgat tgcaactcct atagcaaata
     3121 agatatccaa gtttccaaaa acggcatttc cagctgcacc aattactggg atgttcagca
     3181 tatcgtcagc tgataaacgc atgatcaatc ccgctaatgg catcgctgca actggaagta
     3241 gcatcgctct tccaagcttc tgcacatttg atttaacatt catttttatt cctcctctat
     3301 taaatatcct catatgttac taactcaatc aaaggatcat ttttcacttt ttgagcaaat
     3361 gctgaatgaa ttaaaaattc gacttggtgg gtacgaggat acacaaaact tgaaccattt
     3421 aacaattcct gatccaaata cgctgcgtga cacatgactt cgatcgtttt atcttctctc
     3481 aatatcatga gtaaacttgc caaatattct tccacactcg tattgatttg tcgttttttt
     3541 tcatcagttt ctcctacgac atcgaaacaa ggctcaaaat aatcgacatg acgaacttca
     3601 tcttttcgtt caaagttgct ccgaaccggc aaatcatatt tttttgctaa tgtaacaact
     3661 acctcctgat tttcatccaa agtatgagta tgatgatgac tgtctaaatg agttggtatg
     3721 atccccgcat gataaatttt ttgaatctga gcattccatt cctgatacag ttgatctttg
     3781 tcaatgttaa atggatgctg ataataagat aaggatctga actctccgct ttcgataaga
     3841 gtatcgacag ttttgaggag tggtttgcca cacgtcaatg ttaaatggac tcctacacct
     3901 agcctaggca tttcctttct tagtttcaca gcatgctcga aacccggcat atttgccatc
     3961 aatgtagttg atgtcaaaat tcctcgctga taagcatcca taatcccata attcacacca
     4021 tggctatagc caaaatcatc cgaattaaaa atcacttttc ccatttcatt ctccttcttt
     4081 tttctactaa aataggcgca accaaataat catagtccat ttactatgtg tcatttggtt
     4141 gcgcctatag atagttacgc ccttatttta atagattctt gctagatgaa ttgccaaata
     4201 gatgatttca ttttcttgaa tggcaagttg aaattgctgt tcgatgatat ctttaatctt
     4261 tagtgctgta caatacgctt tgctatgttt aaatgtttca gtaaaaaaac caatattttc
     4321 tactggcgtt tcttcctgat aaatcaagcg tttaaccagg aatttgatat gaaccattaa
     4381 gcgatgataa gtcagtgatt ctttatcata aacgtttgaa taggattttt ctagatggga
     4441 caaaatcgtg gtcgtcattt ctactatttt taagctcttt aaactattag gctctcctag
     4501 ctcagcatta atgatatgca tagtgatgaa tcctacttca tttgacccta actcggtttg
     4561 atatcgatga ttgattcgct cgaccacttc tactgctata tcatattcat ttggataaag
     4621 gaatctcatt tctggcaaca taattgctgt gacatctcta tcttctcttt ttcgttgaat
     4681 agcaaaataa atatgatctg tcaatgtaat atagatattt gaatctaatg gagaggagat
     4741 cctagctgtt atcagttcaa tcacttcttc cgcaaaatcc ataaattcga ttggaatctg
     4801 cgagactatc tcactgaatc gtttgatctt ttctttagaa tctaatacaa ataatttgtc
     4861 agcatcctca acatcaaatg gcttactcac tttagcagaa aagccaatcc ctttccccat
     4921 cgcaattttt tctattccat cgtcatcgat caatacagca ttatgattca gctttttcat
     4981 cacatacacc gttaattcac ctcctctata tgataaaaca aaacaacttg cagactcctt
     5041 catacatatt tttgtctcca aacaattttt aaagacaaaa aacgacctaa gaaagaaccc
     5101 tttcttaggt cgtgcctaat tgaatagtag caccctacat aatttacaaa aatagtttaa
     5161 cacataataa aagcgttatc aatatctttt ttactcgttt tatgataata cttgtatcga
     5221 attgatttgg aggtaattac ataaaaacaa aaaaaatata gcaactctct ataagaaagt
     5281 tgctatattg ataattatac cggcggccgg ggtcgaaccg gcacgtcctc aacggacact
     5341 ggattttgag tccagcgcgt ctgccaattc cgccacgccg gcaaatttaa aaataataac
     5401 tggggtagct ggattcgaac caacgcatga gggagtcaaa gtcccttgcc ttaccgcttg
     5461 gctatacccc aagaataaag ggcgaatgat gggaatcgaa cccacgaatg ccggagccac
     5521 aatccggtgc gttaaccact tcgccacatt cgccatcata aaagatgtta tttaatttct
     5581 caataaaaaa ctaagatgaa gacatcttag tcaataccgg cggccggggt cgaaccggca
     5641 cgccctcaac gggcactgga ttttgagtcc agcgcgtctg ccaattccgc cacgccggca
     5701 agttgattta ttagtactaa ggcggtaacc ggatttgaac cggtgatgaa ggttttgcag
     5761 acctctgcct taccacttgg ctataccgcc attatactat tatcaatcta taaaataaag
     5821 aataaattat ag
//
LOCUS       DANKHL010000173         5814 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000173 DANKHL010000000
VERSION     DANKHL010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.182"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(233..1015)
                     /locus_tag="PW101_002329"
     CDS             complement(233..1015)
                     /locus_tag="PW101_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514708.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1080..1916)
                     /locus_tag="PW101_002330"
     CDS             complement(1080..1916)
                     /locus_tag="PW101_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2514709.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1934..3343)
                     /locus_tag="PW101_002331"
     CDS             complement(1934..3343)
                     /locus_tag="PW101_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2514710.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3357..5180)
                     /locus_tag="PW101_002332"
     CDS             complement(3357..5180)
                     /locus_tag="PW101_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2514711.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1782 a         1264 c          948 g         1820 t
ORIGIN      
        1 aaatttctta aaaatttttt cactaaaatc actacctgaa acagtctaaa acacttatat
       61 atcaacgttt aatgaaacta tctccgtttc atttcagata caaataaata tagtcgggct
      121 ccaaatccga aaaaaaatac ccccagtagc ttatcctaag ctactggaga tgtataagta
      181 cggtcatata acaacggggt agggtaaata aatgaaaaag atggaagaat cattattcaa
      241 taactttttt aaatacttcg ttgtaatcag ttacctgtcc gttcttaaat accacagaat
      301 aatctttttt cccattttca agtaaccgaa gtggaatgtc tttaccaact tgctctgtca
      361 ctgcattact gatacctgtt agattagcta ataaatctga taaagattga gcatctgtag
      421 aaatctctgt atcagtattt atcgtgattc ctttatcttc atttacaagc ttccaagtag
      481 gtggtatagc tgtgtatgtt tcgatgaatg aatcaattct ttcatctatc gtcttcccag
      541 tatctacttc tgtctcatta ctcttagatg aatcgattct ttcatccatc gtctgctccg
      601 taactgcttc tgtctcatta ttctcaccgg aaactctctt cacacccaaa cttatatatt
      661 gtccatcttt agtgtgcata gttaaaattc ccacgctttt actaataaat tcactataac
      721 cagttctttc aatagagact tcaatgtatt tcacactatc atactcaaaa gaaaaataga
      781 catgtaaaag cattttgttg tttacatcta cataataatc ctttgactct acttgagacc
      841 ttcctatacg gaatacagct ttactgtctg atatctctat accagcatcc ttattattaa
      901 tctgccaatc tccaagtagc tcttctggca agctagtagt ttcttgtgaa cactttacca
      961 cttcactggt agaggtcgaa acattcacct ccgcactgtt agaagaattt tccaaggaat
     1021 caggagcctt agcacaacca acaagtcttt atatattgtg cagccgatat tttcttcatt
     1081 cagtacaccc ttgaaatatg cagtgttaga taaagcagtt cgtcctgatt tgctgaggca
     1141 cccatctctt cttgaacgta ctccttgatt ttcaaggccg cccgatactc tttcggaaaa
     1201 cgctgcttca ctgaagccag caaaaaatca tccttctccg gcaactcttc cttcttaatc
     1261 attcgggcaa taaaaaactg taaatgcgtc acaaatctta agtaaagcat cgaatcttta
     1321 tcaacctttt tttcgggaaa cagatatcct agcatagcca agatatcctt tagtgctctt
     1381 accgagagca tggtttcttc catatttgct tgttcacttt gggcatttac aatgtgaaac
     1441 gcaatgtttc cagcctcctc ttctggcaac tgaacattca tttttttgtt gatcaattgg
     1501 atagcatagc agccgatctc aaattctttt gggtaatact tttgaatttc aatcagcagg
     1561 cgattttgaa aaacaatgcc tttttgactg cgttcaatgg catgcttcaa gtgatcaacc
     1621 aaggtaaaaa atagactttt attcagctta aagtcaaact tggcgttggc ataatcgata
     1681 attcgcttga cgcaaataat tagatcatcc ggagcagcct caaacagttt aatgtaatcc
     1741 ttttttagag catcagaaag tgaatctaat ttaaatactt tttggatttt gtcagccgga
     1801 accaaatcgc ctttctttag ttgaaatcca atccctttcc ccatcaaaat tacttctttg
     1861 ccttgcttat ctaagcaaag aattaaactg ttgtttaacg ttttaattac tctcattaga
     1921 ttcaccgttc tctctaagct aagccatttc ttgcgattac tttttgatac caaaagaagc
     1981 tttctttttt tattcgtttt aattctttaa gattctcatc ggttcggtca acatatataa
     2041 aaccataacg cttattgaag ccttgatggg tactaacaac atcaatcgct gaccaaggta
     2101 aataaccaaa aacatctaca ccatctgtta aagctgcttg aatagccaga atatgtttgg
     2161 ctagataatc aattcgatcc tggtcattaa ttcgtctatc ttcctcaact atgtcgggtg
     2221 ccccatagcc attttcagta attaaaatag gtaaatcata gcgatcgtac aatttcctca
     2281 atgttaatcg tagtccagta gggtcaacta cccagccata tggcgtttta tctacataag
     2341 gattttcagc tgcccggtag acaccttctt ctcccaacat gatctgttga tcccccgctc
     2401 tagcggaaac atctgatccg tcatttttgc tggcagcgat ggtggcagtt gaataataat
     2461 taatcgcaat aaaatcgggt ttaccggagg ataaaattgc ctgatcctct gcctcaattt
     2521 taggatataa tccacgatca ttcaaatagt tttcaaacaa ataattgtat tttccccgaa
     2581 ctgctacatc taagaaagac cagcctctca atgtttccca attatgggct gcaattgcat
     2641 cttctggact ggaggtagct tgatacatag ccgttaaatt gattgctgga ccgatttttc
     2701 ctttaagatt caactgatga tacaagtgaa ttactctagc ttgagctaat aaaacatggt
     2761 gattaatttg aaagagttct ttttttgaag gcagctctcc acttggtggc aaaccaatcg
     2821 ctccaggatg aaggatcatc gtgttttgct cattaattgt cagccaatag ttcaccttgt
     2881 caccatactc agtaagcaat acctgtacat agtccacaaa ataatcgata gttgctcgat
     2941 ttaaccaacc gccctgctcc tctagaaaat aaggtaaatc gaaatggtac atggtgacaa
     3001 tcggttcgat tccctgattc aggcattcat caattacttg atgataaaaa gaaattccct
     3061 tttgattggt tttattattt tcaatatcca taattcgcgt ccaagcgatg gaaaagcgaa
     3121 aagcttttaa gcctaattct ttaaacagct gtatatcttc tttatagtga tggtaaaaat
     3181 ccgaggcaac ttcaaaatta gctacccctt ctgtatgctg atacttgtca ataatagacg
     3241 gttgtttccc atcctcctga taggcaccct ctgcctggta ggcactaatg gaggcacccc
     3301 ataaaaactt ctctggaaat gctgaaagct gtttgtattg cacaaaaatc ctctccttaa
     3361 ctaatttgca tcaatggttc gtgttccttt aagcttccgc ttgcttcact aattcctgcg
     3421 taattttcac tgttggtcac taccacaatc actgtatcat cgtagccagc gtttctaatg
     3481 gcagcgaaat cggcgttgat taataattgt cctgctgaca cctgatctcc tgcttttaca
     3541 aatacttcaa agggttcccc ttttaaattc acggtgtcga tccccacatg gatcaataat
     3601 tcaatacctt ctttagaacg tagaccaatc gcatgtttag attctaaaat agtaatgact
     3661 tctccctcaa caggtgaata aatgctgttt tcttctggat gaatggctac gccctttccc
     3721 aatagtccct tagaaaagac agcatcgttc acttcagaaa taggacacac ggttcctttt
     3781 accggatttt caacaatgac tgcttcggct attgccgttt gctctgattc atcagaagta
     3841 tcgtcaaatc caattagata agcaatgata aaggttgcaa caaaggcgat tacacacgta
     3901 ataattgcgt gaacaatatt cattggattt tctccaataa aggctggtaa ggctgctaag
     3961 ccaggggaaa caaaggcata tctgactaaa ccgctcaagc cagcatacaa accagcaatt
     4021 gcaccaccaa tcatggaagc aactaatggt tttttcagct tcaaggttac tccatagagt
     4081 gaaggctcag taattcccat taaagcagtg atcccagtgg acacagctag ctgcttcaac
     4141 tctttgtttt tagttttcgc ggctacagca aaggtagcac ctgattgagc aatattagaa
     4201 gccagcattc ctggcccatt gatcatttca gaacccgtac tgctcaattg catagtagcg
     4261 attggcgtca ttgcccaagc tgtacccgta accaccaaaa atggctgcaa accacccatc
     4321 aaaaatggaa ttaaccaagc tgctttgccg ttaatccatt cagcccccat tgctataaga
     4381 tcgttcagca acgttccaaa cggtccaatg acaaccaacg ctaaaggccc cataattaaa
     4441 aataccagca tcggcttagt aaagaattta ataaacgaag gcgaaattcg atccgcaaag
     4501 ggctctacat aggacatgat ccaaacagta aagataatcg gtaaaactga tcccgcataa
     4561 tctgcagcta ataccggaat tcctagaaaa gaaacggcat cgccagtaga aaaaagctgt
     4621 gaaatatttg gatgaagcaa aatgccagca acagtaatcg ccaaaattgg gttgacatca
     4681 aactttatgg ctgccccata ggctaatagt actggcataa aataaaaggc tgcatcagca
     4741 atagtattca aaatgatgta agtttgacta ctgtctgata gtacaccagt caaagtcaag
     4801 acggccaaaa tggcttttac catcccggca cctgttatag ctggaatcat cggtgtaaag
     4861 gtggtagaaa tgacactaat caatcttgaa ataatacttt cttgattgtc tgaagagggg
     4921 ccctttttaa ttgaagttcc cacttccttt tgaatttcct tgaaggctgt ttggacttca
     4981 ttgccaatca ccacttggta ttgtccaccg ctatccacca cactaattac accaagtgtt
     5041 ttttctaagg ctttcgtatc ggctttcctt ttatctttga gttcaaaacg cagtctggtg
     5101 gcacaatggg ttaagctgac cacattttct tttcccccaa cctgttgcaa tacatttctt
     5161 cctacttctc tgtaatccat tattctctct ccttaaagca gtaaaaaaga ccaagtcaaa
     5221 ccacgcccca atcaacattg aagtgttagt ttgtcttggt cttgcctgca taatcagtaa
     5281 catgccaata acgttatttt gctgtaatcc aataatagca cattatgata gcgcaatcaa
     5341 cagggaattt aattttttat cttttcgttt gaccgtatag ggtaatataa cagttagatc
     5401 acatcaacta gggtatatct gcgtatactt actgctaaaa taagtatatc taatattaac
     5461 tataccagta aaaccattcc tacataacca gcgggaaatt cccaaaatcg tgaaagcatc
     5521 aaaaatgcat ataacggatc acacaacttc aacaattctg ctcttgggaa aaattgatga
     5581 atttggtgag taaaggatgg aataaacagc attccaacaa tataggcaac aatagtgata
     5641 cttgtttttt tggtaaaaat cagtaagcaa tagccaattt caatcattcc gagatataaa
     5701 ggtatcgata gaatgatttg ggctagtatt cttaaaaatt aactagcaaa tgtttaaact
     5761 gatttatttc taatctacat gtagtaagta tttcaattcc accaattatc aata
//
LOCUS       DANKHL010000174         5808 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000174 DANKHL010000000
VERSION     DANKHL010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.183"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(49..2220)
                     /gene="copB"
                     /locus_tag="PW101_002333"
     CDS             complement(49..2220)
                     /gene="copB"
                     /locus_tag="PW101_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HDL2514712.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(2240..4426)
                     /locus_tag="PW101_002334"
     CDS             complement(2240..4426)
                     /locus_tag="PW101_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDL2514713.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(4426..4635)
                     /gene="copZ"
                     /locus_tag="PW101_002335"
     CDS             complement(4426..4635)
                     /gene="copZ"
                     /locus_tag="PW101_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDL2514714.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4648..5088)
                     /locus_tag="PW101_002336"
     CDS             complement(4648..5088)
                     /locus_tag="PW101_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HDL2514715.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5162..5701)
                     /locus_tag="PW101_002337"
     CDS             complement(5162..5701)
                     /locus_tag="PW101_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2514716.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1503 a         1305 c         1015 g         1985 t
ORIGIN      
        1 tcgaaaaaaa ggttgtgaca cgttttttgt cacaaccttt tttcattatt atctgatttt
       61 caatgtcaaa gcatttagag ctacaactac cgtacttaac gacatcaaga cagcaccaac
      121 tgctggactt aatatgatac caattggcgc aagaattccg gctgctagcg gaattgcaag
      181 aatattgtaa cctgcccccc accagaggtt ctggatcatt ttctttcgcg tttgcttggc
      241 tagatcaaga aaatgcaaaa tatcttttgg atcgctgttt gtcaatacga catccgcaga
      301 atcgattgcg atatctgtcc ctgcaccgat tgccatcccg attgaagcac gggctaaact
      361 tggcgcatca ttgatcccat cacccaccat gattactttt ttcccttggt caaggtattt
      421 ctggacaaca gcttctttat catctggtaa taaaccgcca taatattctt cgatccctaa
      481 ataatctgct actgcttggg cagcgttttt attgtcacca gtaagcatga caggagtgat
      541 ttttctttct ttcaatgttc ggataaattc ttttgcttct gtttttacta cgtcacctaa
      601 agcaacgatt gcagctagat gattttctaa aatcaggaag ctgatggtat ttccttgttc
      661 ttggtaaggt ttcaataatg cttgttctac gtttaatccg agacgctcaa cttctttttc
      721 attaactatt ttcacttgtt gttttttgac tgttgcctct agcccgactc ctgaaagatt
      781 ttttagattt tcagcttgat aaggcttgat ttcatgttct ttcaaataat tcataatgcc
      841 aacagccaat ggatgattgg cattcgcttc caaggctcct agatattgaa ggatttcatt
      901 tttattgaac gcatcatcaa gtacttcgac acccgttaca gtaaattttc cttctgttag
      961 tgttcctgtt ttgtccagca tgatcacatc tagatcattg gcttgctcca gtgcattgcg
     1021 gtttttcaaa agtaatccgt ttttagctgc tattgatgtg gaacgagcaa caactaatgg
     1081 gatagctaaa cctagagcat gaggacaggc gatgataaag actgttacca tacgttctaa
     1141 tgcattagga agatctgtca agaacaacca ggcgatgaat gccaggatac ccacaattaa
     1201 tgcgacataa aacagccatt ttgccacttt gtcagataat gactctagct tggatttttc
     1261 tccttgtgct tttcgcacca tttccataac cttcgctaga taaccatttt caccagttcc
     1321 agttacttcg atttcgatcg ttccatcccc attgatagaa ccgccaatca ctttgtcatt
     1381 ggcttgtttt ttgattcctc ttgattcgcc agtcacagct gattcgtcaa caattgtttc
     1441 acctttcaag attacaccat ctgttggcat tttatcacca gaccggacga tcaaacgatc
     1501 accttcgttt acttctttca atgaaactgt ctcttctttt ccatctttcg tcaagcgttt
     1561 cactgattct ggcagtaatt ctgctagttt ttgtaaagca ttgctggcat tcgacacagc
     1621 attcatttca atccagtgac ctagtagcat aataacgatc aaagtagcca attcccagaa
     1681 gaaatccatg acgtgtgtat gtggattgag cagatttgcg ataaacgaat agacactata
     1741 aatatatgca actgtaatcc ccatcgcgat cagtgtcatc atagcaggac ttttttgttt
     1801 caattccatc ttagctccac ttaaaaatgg ctgaccgcca tagataaata gtatagttgc
     1861 taagatcaat acgagccaat cagaaccagg aaacgttact tgaaatggaa attccattcc
     1921 catcattggt gaaaaaagaa tgattgggat agctaggata agcgatagcc aaaatttttg
     1981 tttgaaattc cccatgtgca tggaatgatc catgccgccg tgttcgtgat ccatgtgcat
     2041 cgaatggtcc atttgtttgc tgtttgctat atctccatgg ttatgtccca tgtgcatgga
     2101 atgatccatc tgactatggt catgtttcat ctccatctgt ttttcatgag attctgtact
     2161 ttctttgttt actacttctt ttttatgctc atgttttttc tccatatcgt tcccattcat
     2221 ttttactcca tcccttctct tactttttca tgtgtcgatt taagcacagg gagttcaaca
     2281 atacactgac ggaactaaac gccatcgctc ctcctgcaat gattggattc aaaaagccta
     2341 gtgcggcaaa aggaatacca atcgtattat agacaaaagc ccagaaaaga ttttgtttga
     2401 tttttctcaa tgtcaaccta gacaactcga ttgtttgtgc aatggatgcc aaactgctat
     2461 tcatcagcgt cacatcggct gtttccatcg caatgtccgt tccacttccc attgccatac
     2521 cgacatcagc taatgctaag gccggtgcat cattgattcc atcgcctgcc ataccgactt
     2581 ttttgccatc tttctgaagt ttttctacgt aggctgcttt atcctctgga agtacttcag
     2641 caaagatatg ctttggatca ataccaactt gtttaccaat cgtttcggct gccagtttgt
     2701 tgtctcctgt cagcataaaa acatctaatc ctttattctg gagctgcttg attgcttgtt
     2761 tgacttctag tttgatttga tcagccacgg caatcaaccc gatcacttgc ttttcatcag
     2821 ctaaaaacat gactgttttt ccctgctgtt ctaattctaa cgcgtgttct tggtattcat
     2881 caaatgatag gttcaattca attaatcgtt tacgtgtacc cgcaaaatag cgtactccgt
     2941 taatcgtacc gctgattcca gcacctgggt gggctgtaaa atcaatcata tcgtatgttg
     3001 ctgcttcttg cagttttccg tattccacga ttgctttacc tagtggatgt tcagaagctt
     3061 gttctaaagt ataaaaaata gataggactt ctttagaacc gactagatct gtaactttgg
     3121 gctttccttc tgtaatcgtt ccagttttat ccaaaacaat gctgtctaag tgagcagctg
     3181 cttctaatgc ttctccgcct ttaattaaaa ttccatttcg tgcaccaaca cccgttccta
     3241 ccataatagc tgttggagtc gctaaaccta atgcacatgg acacgcaatg actaatacag
     3301 agacactatg gatcaatgcg agttgccaat caccagtgat caaacctgta gctatgagcg
     3361 tgataaacgc gatgactaat acgatcggaa caaaaatccc tgagatccga tctgcgattt
     3421 tttggattgg ggctttgctc ccttgagcat cttccaccat ttgaatgatt tgcgctaata
     3481 ctgtctgctt gccgacttgt gtaacttcca tgtgaagtag accattcgta ttgattgtcc
     3541 cgccaaagag cgtgtcatcc ggattctttt ctacaggaag attttcacca gtaagcatac
     3601 tttcatctat cgcactgctt cctgaaataa ttcgaccatc tgctggaatc tgctcaccag
     3661 gacggatgac tagttgatca ccaacgacta cttcttcaat cgctaacgtt tcttctttcc
     3721 cgtttcttat cacttgggcg gtttttgttt gaagagacat caattgtttg attgcattac
     3781 ctgttttcgt ttttgctgta tgttccaaat atttgccgag caaaatcagc gtgatgatca
     3841 tactgctgct ttcaaaataa agatcttggg ggtgtccacg gaaaaatccg ttgtaaatgc
     3901 tcaatgcaaa ggcagccgat gttccaattg ctaccaatac atccatattt ggtgctttcg
     3961 tcttgattgc atggtacgca cctttataaa aacgagcacc gacataaaat tggacaggag
     4021 cagacagcac taactggacg attggaaaat gaaaaaagtg aacaatcgcc gcatggcttc
     4081 ctaacatcat agcaatcatt gacaacatca acggcaaagt caaaaccgta ctcaatataa
     4141 gatcgaatag catttttttc aaatacgctt gcttttcttc tgcaattttt tgtttgtggg
     4201 cttcatcgta caaaattgca ccatagccga tgttttctac gctttggatt aatttttcag
     4261 tggtcgtccc ttcatatttg acacttgctt tttcagtggc caagttgact gtggcattga
     4321 ccacaccagg ttgtccgttc agctcttttt cgatcctagc agaacaattt gcacaagtca
     4381 tacctgtaat gacgaacgta tccattttgg tttctgctgt tgccatcaaa tcacctctgc
     4441 tttataaccc aatccattga ttgcttgaca aatcttttcg gattgaactt ttgtttcatc
     4501 aaacttgacc gtaccgtttg cttttttcaa atttactttc actttttgca cgccgtcaag
     4561 tgcgcttact gtttcttcta ctcttgctgc acaatgattg cagctcatac cttcgattga
     4621 aaattgttgt ttcatttttt cttcctccta ttgatgttgt ttacattcac attgacctgg
     4681 gatacagtta cattcgatcg tatctactgg ttgcttttgt tccagttgtt tcaaaatctc
     4741 attgatatct tcttgagtca aagtggcttc ttctactaga tccgcaatcg tctctccaat
     4801 cctttttgca cagatatggg aaaaaagatt ttccgtcgca ctgcgaactg tttccatctc
     4861 cgagacagct ggataataaa tgaatttttt tccttcttgt tctgtccaga tcacttcttt
     4921 ttttaccaaa cgacccagca aagttttgat cgttgctggt ttccaatcca tcgaatcagc
     4981 taagatctgc gtgatttctt gggctgtcgt ctgtccaagt gtccaaataa cacgcatgat
     5041 ctcccactct gaatcactga tctttactgg cgttttgact ttttccattt atttcacacc
     5101 tttcgtttac aaatgtaatc attatcttat gaatacagat tacacttgta aacaaaaact
     5161 gtcaattatt tttgctctct attttcaatt tagttaaaaa ttcttgtttc tctcgtcttg
     5221 ttttaaggat gatcagttta tttcttgcat tagctacttg gatcagctgc tgtattttcg
     5281 gttcattgtt ttcttcaaaa tgccagataa aagataaaaa ttctaagtat tccctgttta
     5341 gtttctccga aaatgattca ggcatatctg gccgagattt tttgtttatc tttcttttta
     5401 gagaacgcca aactactcgg tagatggcga gataacgcgg cactttaagc caaatgatct
     5461 tatctgcttc tttaatcctt tcctctaatg tagaagtata atttccttca acgatccatg
     5521 acggattaga taccataaac aattgctgtt cttgcaaaaa ccactttttt gcctgaatac
     5581 tgtagtctgt tgtatgccat aaaaggtcta gcggcagtag cggaaagtcg gtgatttctt
     5641 tgatcttcct tgcgagggtc gattttcccg acccactttg tccaatcacg atgatcttca
     5701 ttcttctccc tcacttctct ttggaatttg gttcgatcat accacttata acgccgcaaa
     5761 aaaacaaggg actgtgacaa aagtcgtgtc aaagtccctt gtttcgaa
//
LOCUS       DANKHL010000175         5788 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000175 DANKHL010000000
VERSION     DANKHL010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.184"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            112..1008
                     /locus_tag="PW101_002338"
     CDS             112..1008
                     /locus_tag="PW101_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDL2514717.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1158..2099)
                     /locus_tag="PW101_002339"
     CDS             complement(1158..2099)
                     /locus_tag="PW101_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL2514718.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2225..3346
                     /locus_tag="PW101_002340"
     CDS             2225..3346
                     /locus_tag="PW101_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL2514719.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3604..5106
                     /gene="eat(A)"
                     /locus_tag="PW101_002341"
     CDS             3604..5106
                     /gene="eat(A)"
                     /locus_tag="PW101_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL2514720.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5134..5700
                     /locus_tag="PW101_002342"
     CDS             5134..5700
                     /locus_tag="PW101_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2514721.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         1891 a         1025 c         1220 g         1652 t
ORIGIN      
        1 tttcatacaa atgttaaatt ttcatacaaa tataggaaac gttttactgg ctttttacta
       61 tttaaacagg ctatcatagg aaaaagtgac agagaaagtg aggcggaaaa gatgaacaaa
      121 aatgacgtac tgctagttgc aaaaaataaa tcactttatt ttttgccttc catccgctct
      181 ttagatgttt ggtccggaaa agatttacta cagtcggaat tcattaaatc agctagacaa
      241 cagttggcaa attatcagct gatcgttttt ttagattatg gatttgatat ggagatggct
      301 cgacgcgtgc gcccatttac caatgcaaaa attgtgttat ttttttggaa tcacttcaaa
      361 gcagaacacg aggcgatgct gaagcaggca caagatgaac cggcaatcga tgagatttat
      421 cattttgatt ttttggaagc aaaagaactt gggctgaaac ataatagttc attttattcg
      481 aaacatatga atttttcttc tagcgaacca aagacggatc tattttttgg tgcaacgaat
      541 aatgggagaa aagaacgagc agaaagctat aagcaggaat ttataaaaag agatgttacg
      601 accaactatt tcattcttcc acatcgaggg aatgaacaac ctggatatct tacttatgaa
      661 gagtacctga aaaaaacagg ggaatcgcgc gggattctgg aactgttgcg ggagggtcag
      721 caaggagtga ctttgcggac atttgaatcc atgtattttc aaaagaaact agtgacggat
      781 aatcaagcga ttgctcatta tcatttttat cgtcctgaaa atattttttt gcttcgagaa
      841 cgtgatttag atgaattacc ggaatttctg catacaccat ttaagccaat cctatcagag
      901 ctattggaat tttatgatgc agaaaattgg gcaaaacgat ttatcactaa cgattccact
      961 atttatgagc aatacgaata tgacctctcc tctgttgaaa acaaataaaa aacctacatt
     1021 ttgaagagat tatgtactga aacaaacaag cacaattctt atggcatcat gagagttgtg
     1081 ttttcttttt gatcttttat tgggcttaaa aaaagagaac cagctgctga ttcagctgat
     1141 tctcatcaga gagcctttta ttctgcgcca aggattcctt cttttgcatg gactaattgg
     1201 gtcaaaaagg cacaatatgg tgtagcaata tcatattttt gtccttttcg ccaaatggca
     1261 ccattgatgt agtcgatttc agtcaaacga tggtttttga tcaggtcttg gtacatagat
     1321 gggtaatgca acccgatgtt gtcaggatca taagtagaag caatatgttg gtacacttct
     1381 tcttgatcta gtatgattcc ttcttttgct gcgatagaag caaattcaga tacgattgtt
     1441 cgaaccatgc tctctgccgc tttttgcgcg cctagttcag caatattaca atccaaaatc
     1501 gtgcataaac cattcaatgt tccatttaca caagcttttc gccaaatgga ataacgaacg
     1561 ttatggctat agatcggatt taaatttgct tcttgaaaga cttctacaac tttttttgta
     1621 aatgcttcgc cttctggttc caagttctct agttcgattt ctccatcacc taacaatgtg
     1681 acttttccgg gaccagctag accagctgtc cacatcgtga tcccaaataa gatatttttc
     1741 tttggcacat atttttccag tacatcttca tgacctaatc catttagtaa acacaaaaca
     1801 taagtattgt cagtaataat ggattggatc gaacaaaaca tatcatctaa ttgattggct
     1861 tttgttaatg ctacgatcaa gtccacgtgt tcgttgcttt cgatgatttc ttctggtgaa
     1921 tagattggca gttttgcgac cacttcttta ccgttaaagt cagcaatcaa cccatttgta
     1981 cgaatggctt caatgtgagc aggccaacga tcgatcaaga ggacctcatt tccgctttga
     2041 tgtagcatca atccgattcg gctgcccatt gctccggccc cagctattgc gattttcatg
     2101 ttttttctct cctttattca atgagttccc ttttagtata tagaaaaatg gaagaaaata
     2161 gcattgtttt gtttgaaaaa aatgaaaaaa agtgtcttac ttaagaagaa agtgagggat
     2221 acagatggat attttggcgg caattgattc atttaaagga agtgcttctt catttgaact
     2281 aaatcaggca gttctaaatg gctgggaaga tcgtaaagga agaaaaatca atgtgccgat
     2341 tgcagatggc ggagaaaata ctgcagaagc aatctattac gcgctcaggg gaacatggcg
     2401 taccattgat gggatggatc tcttattccg tcagcgaaaa atcagctatt tattgacaac
     2461 ttggcaagga aaaaacaccg cagtcatcga atctgcacaa gcattggggt tagatctact
     2521 tgataaacca acggatcaaa ctatccgact tgcttccagt tatgccttag gagaacttat
     2581 taaagatgca ctgcttcaag aagtagaaca aattattgtc actttaggag gaagcgggtg
     2641 tagcgatggt ggtctaggat tactccaatc cttgggtgct tctttagaag ggtacacaga
     2701 aggaaatcct ttattatcta ctaaaaaact tcattttgat cgaagaaacg ttcttgtttc
     2761 taaagtaaaa ttgattatcg ctgcagatgt taccaatcca tatacaggtc aacaaggagc
     2821 tgcttggcaa tttggaagac aaaaaggagg aatagatacg acgctagctt tcttagataa
     2881 acaagcagga aagattgctc agcaaataaa agatttctgg gaggtggatt tagatgatat
     2941 tccaggaagt ggagcagcag gcggtattgg tggagccctt tttttgttgg gagcgaagat
     3001 ggtttctggt ttcgatttag tcagccaaat cactggattg gaaaaaaaga tcgaacaagc
     3061 agatgtcatc attacaggag aagggcggat cgatcagcaa acgatccacg gaaaagttcc
     3121 atacggtgta gcagtgcttg caaaaaaata tcgaaaacca ataatcgcta tttgcggaag
     3181 tagagaccaa gagctagaag gactttctga ttttctccca attgtcttaa gtattcattt
     3241 gggtccaata ggtttagaag aagcaatgga acacaggcaa acattagaaa aaacaaaaat
     3301 cttaggacag acacttagca gatttttcac catcctcccg aagtagttga caaaatcatt
     3361 ttctttatgc tatcgtaatt tcaaatatag gacaaagtga gggatccgtg cattgaaaaa
     3421 ttaagtcgat tgatgaaagc tgcattcaga ctgtaccttg tggagacaag gaagcagtgt
     3481 gcagattttc gggattggct tggttttttc gatgtatggg tctttttgtg tgtatccaac
     3541 ccttgcatcc tctttagcca gttctgaaaa taggactggt ctttttttac gaggtgatgg
     3601 aaaatgtcta aaatcgaaat aaaaaatctg acattcggct acgacagcca aggcacatta
     3661 ttatttgaac aagcaaatct aaattttgac acacaatgga aactaggact tatcggacga
     3721 aacggtcgag gaaagacaac tttactgaat attctacaaa acaaactacc ttatcaaggg
     3781 caagtaatcc atcagcaaga atttgcctat ttcccgcaac agacaaaaga taaagaacgt
     3841 ttaacctatt acgtgttaaa tgatattacg gattttgaga tatgggaaat cgaaagagag
     3901 ctccaattga tgcaaacaga tcctgaaatc ttatggagag aattcagcac actatcgggg
     3961 ggagagaaga caaaagtcct actggcactt ttatttgtgg atgacactca tttcccgtta
     4021 atcgatgaac caacgaatca tttggatatc tctggtagaa aacaagtagc ggcttatttg
     4081 aaaaagaaaa aacaaggctt catcgtggtc agccatgacc ggggatttat cgatgaagta
     4141 gtggaccatg ttttagcaat cgaaaaaagt caactggaac tttatcaagg gaatttctct
     4201 atctatgaag aacagaaaaa acttcgtgat gaatttgaaa tggctcaaaa tgaaaaattg
     4261 aaaaaagaag tcagtaggct aaagaaaaca gcagctgaaa aagccgaatg gtctcgttcc
     4321 cgagaaggag ataaaacaaa gaaacaagtc ggattcatcg atactgaatc tagacgagtg
     4381 aataaaggag cagtgggtgc tgatgctgca cggacgatga aacgatccaa agcaatcgtg
     4441 aatcggatgg agacccagat cagcgagaaa gaaaaactat taaaagatat cgaatatatc
     4501 gattctttga cgatgaatag ccaagcgtct caccataagc gacttttaag cgtagaagat
     4561 cttcaattag ggtatgaaaa tctgttattc gagccaattc attttacaat cgagcctcat
     4621 cagcgggtgg cgatttcagg tcctaacggt gcaggaaagt catccattat ccattatctt
     4681 ctgggggcat tcaacggcaa ggttatagga gaaaaatacc agccaaaaca tctgagcatt
     4741 agttatgcaa gccaaaatta tgaagacaat cgaggaacgt tggcggaatt tgcagagaaa
     4801 aaccaagtag actaccaagc atttttgaac aacctccgaa agcttgggat ggaaagagat
     4861 gtttttcata acaagatcga gcagatgagt atgggccaac ggaaaaaagt ggaattggct
     4921 aaatctttat cacagccagc tgaactatat acatgggatg aaccattgaa ttatttggat
     4981 gtcttcaatc aagaacaatt agaacaactg atcttgaacg tgaaacctgc catgttacta
     5041 gtggaacatg atcaaacctt cctggataaa gtatctactg agattatttc tcttgagaga
     5101 atctaaccct tgtaatctca agaacaacaa ggtatggtta aggggaactt tgccaacgaa
     5161 ggagagaatc acatgcttta tgtaacgaga cacggagaaa caacttggaa cgcacaagga
     5221 ttagtttgcg gacgggcaga tgtgccgctg actgaaaaag gacagatgca ggcacagaaa
     5281 ctagcagaaa aggtagtaga tttgcctgtt ccaatcacga aaatcatcca ttcccctctt
     5341 caacgagcaa gagatacagc acaagcagtt gctgatcgtc tcagcttgcc gctgaccgtt
     5401 gatgaacgat tggtagaaat ggattttggg gattatgatg gactgccaag caaagatgag
     5461 aactttcaaa aagcacgttt agcgttcgct gtacgattcc caaatgggga gtcagtctta
     5521 gatgtgtatg cacggatcgt tcctttattg aaagaatgca tcgaagacga agaaaatgtc
     5581 tatctgttag tttgccataa tgccttgata agagtgatca atgcttattt tcatccgatg
     5641 cctaatgagg ggttctttac attcatggta gataatacag aattggtttc ttatgagtaa
     5701 ttatgttttt gtgttattta aataaaatat ttcaaggcac tgcttttgct tagcagtgcc
     5761 ttttttaggt tatgacaatt cttttgat
//
LOCUS       DANKHL010000176         5780 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000176 DANKHL010000000
VERSION     DANKHL010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.185"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            290..1282
                     /locus_tag="PW101_002343"
     CDS             290..1282
                     /locus_tag="PW101_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2514722.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1342..2763
                     /locus_tag="PW101_002344"
     CDS             1342..2763
                     /locus_tag="PW101_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDL2514723.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            2756..4255
                     /locus_tag="PW101_002345"
     CDS             2756..4255
                     /locus_tag="PW101_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDL2514724.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4294..5688
                     /gene="uxaC"
                     /locus_tag="PW101_002346"
     CDS             4294..5688
                     /gene="uxaC"
                     /locus_tag="PW101_002346"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL2514725.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         1901 a         1068 c         1183 g         1628 t
ORIGIN      
        1 tgacgttttg gtcgactaaa ttttaccaaa agtgagtctc tttttgagtt gaaacgaaga
       61 aaaaagtgtt agtagaaaat cgctttcgat tttccaccaa cacaaaatat tatagaaccc
      121 taattaaatg agcattcccc gctttcaaga atagcataat taaaaataaa aatccaagta
      181 atttttaacg tgtgcaatct tttcatatca aagatctatt gacttatcgt agttatcgta
      241 atatgatgaa tgcgttaaca aaaagtgaaa aagtaaggaa aatgatggaa tggtaacgat
      301 caaagatatc gctaaagcag ctggcgtttc tcatacgaca gtttctcgtg ctttgaataa
      361 cagcagttta ataaaaaatg aaacaaaaca aaaaatacga caactagcta aggaaatgaa
      421 ttattcgcca aactatagtg caaaaggttt agtgaatcaa aaaaaatatc taatcggact
      481 atttttctca agtttgcata aaggaacttc atcaaatttc ctagcagatt ccattgcagg
      541 catccattca gtattagaca caaattacag tttatctgtt gaaagtatcg atgttgttga
      601 tttaacagaa atcaacctgc aacgctacga tggactgatc gttatgagtc aaagtgatga
      661 tgatcagcct ttcatcgact atctcaaaga aaaagaattt cctttcgttg ttgtcaatcg
      721 tcacatcaat gatccagcgg ttatcaacgt cactgccgat gatgcggccg gtgttatgca
      781 ggcagtagat tacggtattg gatgcggaca tacgaagatc gcttatattg gcggaaagaa
      841 aaattttcgt tcgacaaaag aacgggaaaa aggggtattg aaaagtctaa aaaaagcgag
      901 catccctgtc aatctagcct attttttaac aggagattac agcttagaaa gcggcctcca
      961 aaacatggaa tacctattat cactttctga cctgcctact ctggtttttt gcggcaacga
     1021 cgacatagcg attggtgcat tgagagctgt acatttagct ggcctccgtg ttccccagaa
     1081 catttctctg atcgggttcg atgattctcc agtcgtctcc tatctcaatc cgccgcttac
     1141 caccgtacgt aagcctttga aagaaatgtg tcaagaagga acaaagcttt tactagattt
     1201 gatcgaaggc aggacgatcg aacaatctaa aattgagctc tccaccttgt tgatccttcg
     1261 tgagtcgatc aattacttgt aaactacaag taattttata taaaaatgtt aacgtgtgca
     1321 taacacagaa aggaagttta tatgaaaaaa ctgacaaaag aattagtgaa aaaaaacgaa
     1381 gcacctgtaa aagttcttca gtttggtgaa ggcaatttta tgcgtggatt tattgattgg
     1441 cagattcagc agctgaataa ccaacaattg tttaaaggta atgttgcaat cattcaacct
     1501 ttagaacaag gtcttggaaa tatgatgaac cagcaagata atttatatac ggtcatttta
     1561 caaggactca gcaatggcga aatcgttgat acttccgaga ttatcaccgt agtagaacaa
     1621 atgatcaatc cttatgatga atgggatgct tatctatctc tagcagaaaa tgacgacttg
     1681 gcctatatcg tctccaatac aacagaggca ggaatccaat tcgtggagac tgataattta
     1741 acagatactc ccccacagag cttcccgggc aaactgacag cctttttata ccgacgtttc
     1801 aaattaaaca aagctggctt taccatcatt ccttgcgaac tgattgacag aaacggcgaa
     1861 cgtctaaaag aaatcatttt acgctacgca gaaaaatggg atttagaacc tgcttttgtt
     1921 gagtggttag aaaaagaaaa tgttttctgt tgtagtttag ttgatcgcat cgtcccaggt
     1981 tatcctcgcg aagatgcagg tgttttcaat caacggcata actatgaaga tcaattgatg
     2041 gtcaaagcag aaccattcat gctttgggtg atcgaagggc ctaaagaatt ggagaatcaa
     2101 ctaccattca aacaggctgg cttgaatgtc atcatcacag atgatatgac tccttatcgt
     2161 gaacgtaagg tgcatttgct taatggaccg catacagcga tggtccctct tgctttgttg
     2221 gcggaattgg aaactgtaga agaagtaatg aaagatcctt tattctccgc atatgttgat
     2281 cagttattca atctagaatt gattcctatg ctttctttac ctaaagatga attagccatt
     2341 tatgctgacc aaatcaaaga acgtttcttg aatccttttg cccatcacaa attagaagcg
     2401 atttcattaa atagtgtttc aaagttcagc acccgtctgc ttccagtgtt caagaagtat
     2461 atcgaagaac aaaatcaggt accaccgtta atcactgttt cactagctgc gctacttctt
     2521 atgtatcgag gcgatcaagt aaagccacat gatgatgaaa agacgataag cgaatttact
     2581 gatgcttggt ctgataatgg aacagctatt cctcgattat tgcaaaacgc tgcgctttgg
     2641 ggagaagatt tgtctcaaat accgaatgtg acagatacag ttcaagaaat agctgcaaag
     2701 atcgatcaaa aaggtgtccg gtcagtatta caagaaatga aaggaacgat ggtccatgct
     2761 tgaaaccctg caaccaacaa tgaaattaaa tcgacgagac agtgtggtcg tcgcattaac
     2821 ccctattcca aaacaaacac aactgacgat tgatcagcaa acgatcacca ctcttgaaga
     2881 tatcccgcaa ggtcataaga ttgcattggg tgatttgaaa gctggagaca acgtgatcaa
     2941 atatggctat ccaatcggtc atgtgacgac agacgtaact gccggtcaat ggctgcacac
     3001 tcataatgta aaaaccaatc tttctggtga attggattat cgatatgaaa aagatgtcca
     3061 tccaagtcat tacctttttg agaaccgaac atttcaaggt tacttgcgaa aaaatggaaa
     3121 agtagggatt cgtaatgatt tgtttttagt acctaccgtt gggtgtgtga atggcattgc
     3181 tgaactgatc gtcaaacagt tcaaggaaaa acatccagat ctgggacagt ttgaccatat
     3241 tacgatttta aagcatcctt atggctgttc gcagcttgga aaagatcatc aaaatactcg
     3301 agaaatttta gcggacgcag taaatcatcc caatgccgga ggagttttag tatttggatt
     3361 aggttgtgag aacaatacag ttcctgaatt caaaaaaatt ttaggtgaat atgatgaaga
     3421 acgagtgaaa tttctagtgg ctcaagatgt ttacgatgag atcgaacaag gtgtggctct
     3481 attagaagag cttttaacag cggcggaaaa tgaccagcgc atatcagtcc cgctctctaa
     3541 gttgaatgtc ggtttgaaat gcggcggttc tgatggtcta tcgggaataa ctgctaatcc
     3601 tttacttggt gcattttctg actacttgat tgctcaagga ggcagcacga tcttaacaga
     3661 agtgccagaa atgtttggcg cagaacaagt attgatggca cgagcggaaa atgaagaagt
     3721 ttttgactca atcgttgact tgatcaatga cttcaaacag tatttcttgt cttacgggga
     3781 accagtttac gaaaacccat ctcctggtaa taaagcaggt gggatcacta cattagaaga
     3841 taagtcttta ggctgcacgc aaaaatcagg aagctctcct gtcgttgatg tgctacaata
     3901 tggcgaaaaa atcagaaaac cagggcttag tttattgcaa gctcccggga atgatcttgt
     3961 agctgcctct gctcttgctt catccgattg ccagttggtc ttgtttacca caggaagagg
     4021 gactccgttt ggctcatatg tcccaacggt caaggtatct acgaatacaa ctctttttga
     4081 acgcaaaggt cattggatgg attttaatgc aggcgtctta ttagaacaac caatggaagt
     4141 aatcttagaa tcgtttgttc aaaaaatcat cgctgtagct agtggagaag aaacgaaaaa
     4201 tgaacaaaat gacgttcgag aaattgccat ttttaaaaac ggagtaacac tttgattttc
     4261 ataaaacagt ataaaaagaa agaaggaaga tttatgtttt tatctgatga ttttttatta
     4321 aaagacgatt gggcgaaaaa gctttaccat tcctacgcaa aaaacatgcc cattattgat
     4381 tatcactgtc acttagatcc aaaagaaatt tacgaaaaca acaatttcag caatctgaca
     4441 gaagcttggt taggaggcga tcattacaaa tggcgcttga tgcgagcttg cggcgtgcct
     4501 gaagaaaaaa tcacaggaaa tgcttctgat tttgaaaaat ttttagcttg gtgtcaaacc
     4561 gtaccaaaaa ttatcggaaa tccgctttac agttggtcac atcttgaatt gaagcgtttc
     4621 ttcggtattg acttgttgat aaatgaagaa aatgcagaga aaatttggac acaagcaaat
     4681 gaaattttgg cagctcctga ttttcgccgt cgggaaatcg caaaaaaatc gaatgtagaa
     4741 gtgatttgta caacagatga tcccgcagac gacttgcact atcatcagtt gcttgctcat
     4801 gaagaaccag atttgaaggt tctaccttca ttccgtccag ataaagcact caatattgaa
     4861 aaagaaggat ttaatgcatg ggtcacacta ctggaagaag cagctgacca aacgatcacc
     4921 acctatcaag aactgataga agtcttaggg cagcggatag aatacttcca tcaacatgga
     4981 tgtcgtgttt ccgaccacgc tttagacatc ttacgctata aagaagcgga tgaaacagta
     5041 ttagaagaga tttttcaaaa agcacgagct aacaaactat tatcttcaga tgaaatcgat
     5101 gcttatctta cagaaacact gatacatttg acccacttct atcattctca taattggacg
     5161 atgcagctgc acatccatgc ttatcgtaac tgtaatacac aaatgttgga aaaattaggg
     5221 cccgataccg gctatgatgg aatgaatgat ctttctttga ctattcctct gcaaaaatta
     5281 ttgaaccgtg cagaagaaac ggatcagttg ccaaagacga tcctttattc attgaatccg
     5341 aatgactatc ctgcgatttt agctttgatg ggctgtttcc aaaaagaaca gaatggaaaa
     5401 ctgcagttag gttctggatg gtggtacaac gatactcgag ctggtatgcg cgatcaattg
     5461 acacaatttg ctgatggtag tgctttgggg aattttgtcg gcatgctgac agattcaaga
     5521 agtttcctgt cctacactag acatgaatat ttccgtcgtg ttctttgtga atttttaggc
     5581 gaaatggtca tgcgaggaga agcacctgaa gatgaacaac ttttaggaag tctagttcaa
     5641 gcgattagtt atacaaatgc taaaaattac tttggctttt ttgcctaatt gattaatagt
     5701 aacagtaacg aatcaaaaac aggatagtga aataacggaa tccgtttatc cacgttttag
     5761 agaagaagac ggaccaaatt
//
LOCUS       DANKHL010000177         5667 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000177 DANKHL010000000
VERSION     DANKHL010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.187"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(13..765)
                     /locus_tag="PW101_002347"
     CDS             complement(13..765)
                     /locus_tag="PW101_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2514726.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(1129..3795)
                     /locus_tag="PW101_002348"
     CDS             complement(1129..3795)
                     /locus_tag="PW101_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2514727.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4136..5587
                     /gene="cls"
                     /locus_tag="PW101_002349"
     CDS             4136..5587
                     /gene="cls"
                     /locus_tag="PW101_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL2514728.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         1641 a         1192 c          991 g         1843 t
ORIGIN      
        1 aaaaagaacc cttcacgagt cgtgaaaggt tcttttttta tctgcggacc aaaggagcag
       61 tctataggtc agcttagtcg tactaaggat ttctcgtttt tcactgaata gtttgcgaat
      121 gtacctggtc acaacaggat cttttgcgta cgcgtcatag tatagcttca tcaattcttc
      181 agtaattgat tcctgtgtaa aataagtaaa atcttttttc ttcactttga atccttcatc
      241 aattaagtat gtttcaaatt gatccattac aactggcgat gtattaggat cttcttctat
      301 cattcctagt tgcttttcga taggataaaa aacatcatcg attgattcca ctactctgat
      361 tatcacgcac catttgcttg actttttcat caatttttct aattgaataa gatgtaccaa
      421 tgctggattt gctgctgtaa aaaccatttc atatgtctca tttgattgga taaattcttc
      481 taacgactgt ttgataaagg aaacatttgg caactcattt ttttctgctt ctttttttgc
      541 attttctagc attcctgaag aaaaatcgat caaatcatag cgattaattt cttttgctaa
      601 ggaaagaaat ctgccagatc ctccagcaac gtctgctgct gtttcagcag gcaggacttt
      661 ctccgttttc agataatttt ttacatcttc agcaatagtt acttgcgatt cttgctggat
      721 ttcataatat tcttcagcaa attcatccca ttctttttca ttcatttgat ttcctctaat
      781 tttttgttat atgttttcac ttttcggaat aaatcattct cctcttttat ctattctatc
      841 tcttttgatg aataataaaa tgattggctt tccgtctatc ataaataaaa aaagggactg
      901 tgaccaaagt cgtgtcaaag tccctctttc cgaataaaca gtgtccaatc agatgaccct
      961 cagtattagc tcaaaatgct gattttacaa cttcttcctt aaacggtgtt ctcctgccaa
     1021 ctcttcggtc acaagtcaca atattcaaca aacatgagaa gctgtttgtt gaaattgcac
     1081 cttgtgactc agaattaaac ggcaggctca caacctcgtt gattcgtttt atttatttcc
     1141 ttgtactcgt acaatgatca atttagtgat ttccatcaaa acaattgtca caagtgcgat
     1201 tcccatacat gtaaagactt ctgtcaatcc aaatgctgat gggatagaga agacttcttt
     1261 cagtcctggg atcattgtta agcagtaaag cgcgctacat acaactacag ctagtaagac
     1321 acttctgtta gagaagaacc ctgcttggat agctgtttgt gtgtttgaac gtgctgggaa
     1381 tgtttgcaat gtccgacacc aaatcaatgt tgaaaatgac atggctacac cgaattcagg
     1441 tgaatgctgc atgccaagcc attgagcaaa gataacagca atcccaatca gcacaccacg
     1501 atacgttaca gaaatcaatg ttttgtccgt aaagattcct gaatttggat ctcttggcgg
     1561 gcggttcatc gtagcaggtt cagctttttc tgtacctaat gcaattgctg gcagtgaatc
     1621 attgactaaa ttgatgaaca acagttgtaa tgctgtaaat ggattggacc agtcagcaac
     1681 taaggcaaag atgatcgcaa tgatcgcacc taaatttcct gcaaatagat aagaaatagc
     1741 tttcttgata ttatcaaaca cattacgtcc tacttctact gcgctgacaa tagaagcaaa
     1801 gttatcatcc gtcaatacca tcgctgctgc atcttttgcc acatctgtac ccgttcccat
     1861 agcaattccg atgtctgctt gtttcaatgc tggtgcgtca tttacaccat cacctgtcat
     1921 agctgagata tgacctttat tttgccaagc acgaacgata cgaattttat tctcaggtga
     1981 aacacgtgca taaacagtga ctttttctaa aacttcgttt agttgatctt ctgtcaattc
     2041 atctagttcc gtcccggtca aagccaggtt tccttcttca aaaatcccga tttgttttgc
     2101 gatagcaaca gctgttgttt tgtggtcacc agtaatcatg attgttttga ttcctgcact
     2161 tcttgcttct gttaccgcct gagtcacttc agcacgtgga ggatcgatca tagcaagcaa
     2221 accaacaagg atcaaatcgt cttcatcttc aaatgataaa tcaaaattat caagtggacg
     2281 gtaagcaaaa gctagtacac gtaatgcgcg tgttgaaaaa gcttcatttt gctcttggaa
     2341 ttttttcatc aattcatccg tcatcggaac gacttcacca tttagcaata cttttttact
     2401 gcgttggaag acgatatctg gtccgccttt aacaaacaag taatttttgt tatcaattgt
     2461 gtgtccagta gacattaatt ttcgatctga gtcaaaagga agttctgctt gtcttgggta
     2521 gttgtttcgg atttcctgat aaggctggtt taatttttca ctaaagtcga taaacgctac
     2581 ttccgtaggg tcgcccaatt tagtaccgtc ttcactgatc gatgcatcat tcgctaaaac
     2641 actgatttcg atcaagcgac gttcgtcagc tgtccatttt gaagggtcat ttgtgaaatc
     2701 cccagtatcg ccattaccta aaaagtagtc aacaactgtc attttgtttt gtgtcaatgt
     2761 accagtctta tctgtacaga tgatacttgt cgaaccaagt gtttcaactg ctggaagctt
     2821 ccgaataatg gcatgacgtt ttgccatttt attcgttccc aaagataaca cgattgttac
     2881 gatagactgt aatgcttctg gaatagcagc tacggctacg gctacggcaa acatcactgc
     2941 gctgacgata tctgcagtca tatctcctgt accttctcca agataaatac gaaacagttg
     3001 gatacctaaa atcaataaag acaatgccaa aatagccaaa cttagttttt tactgaattt
     3061 atccagacct cgttgtaacg gagtgacttg ctcagttgtt gattccaaaa ggccagcaac
     3121 ttgtccgatc tccgtattat tcccagttga agtcactaag aatttaccgc gtccgtaagt
     3181 tacgatcgtt ccactgaata ccatatttgt acgatctccg attggtaccg tacttgtgat
     3241 atccgttact tctttttctg cagaagtcga ctctcctgtc agcatacctt catctacttt
     3301 taatgatccc gcatctagta aacgtccatc agcaggtaca taatcgcctg cgtctaacaa
     3361 cacgatatct cctaccacaa gttcgtttgc cggaatcgtt ttttccttac catcacgaat
     3421 cactcgagca tcgggcgctg aaaggttttt caacgcttct aaagaacctt ctgctttttt
     3481 tgtttggaca acactgatta tagaattcaa catcaatact gcaaaaataa ctaagaattc
     3541 gatgtagtca cccatgacca tctgaatcag tgcgacaatc agcaatacga taaccattgc
     3601 atctttgaaa gtttctaaga acaatttcca agttggcttc ttcggctttt cttttagctg
     3661 gttcaagcca tctctttcta atttttcctt cgcttgcgtg gtgctcaatc catcggttga
     3721 gctctgcgtt tgctgcatca acgagtcgat cgtttcttta tagaccgctt gttgtgcttg
     3781 ttccttgttt tccaaatttc atcccccatt ttttgatctt ggccctagtc ctgtctgaaa
     3841 aaaaagagac ctaagacaaa tacgcatttg tcttaggtct caccatttca tacaatacca
     3901 gaaaaccatt tggttaactt actgttgata ttatcgcaac aaacgttgcc ggttactccc
     3961 ctaaggagat attcaactgt actaatatcc taccttatta acctacaaaa agcaactact
     4021 ttagcccgat ttacctacat tttcatgaaa ggagtgaaaa gtatattcac cttggtaggc
     4081 ttctttcttc tatcatgcta tactaatgtt attgtatgta aggaggaaca tttttgtggt
     4141 atctagtatt ataaccgccc tttatctatt aaatgcactt attgctttgg tggctatttt
     4201 gatcaaaccc cgagatgtag cagccatttg ggcatggctc ttagtattgt ttgcccttcc
     4261 tggggtgggc tttgtattat atttattttt cggacgtgga ttaacggata agaaaaaatt
     4321 ctatctccga caaagtgact tgaaagaatt agaaaacttt cagtctttta gaggagatac
     4381 cattgaacat tacgatcctg acatgggcga taaagacaaa cagcaatttg ttgacttctt
     4441 ctcttcatta aatcgtatgc cgctgacaag aatgaattct gtcactcttc tcacagacgg
     4501 acaagagaaa ttggattcac tgcttcaaga tctaaaaaaa gccaaacatt cgatccatat
     4561 cgaatattac gcatttgtga cagataatat cggccagcaa gtcttacatg ttttagaaga
     4621 aaaagccgca gaaggcgtgg aagttcgaat attatatgat gcatttggct ctcatggcac
     4681 aaaagcaaaa gatttcaatc gtctaatcaa aaatggtgga catgtccata catttgttac
     4741 ctcacaaagg gcattacttc gtttccgatt gaattaccat gatcaccgaa aaatcgttgt
     4801 gatcgatgga aagattagtt ataccggtgg tttcaatatt gccaatcaat atgtaaatac
     4861 aacaaaaaaa ttcggctatt ggcgcgatac gcatatacgg attttcggtg ccgcttcttc
     4921 tttgctccag cttcgcttct taacagactg gaacgtctcg gtacctgaag aaaaaaaggt
     4981 cggctatcat ttgaattatt tctttaaaaa agcagatcga gatgaatcta agcttgctga
     5041 tacatccatc cagcttgttt caagcggacc gaataacgaa agggaacaaa tcaagctttc
     5101 atttatcaaa ttgattactt ctgctaaaaa acgtgtttgg atacagacac cttaccttgt
     5161 tcctgatgaa agtgtcattg ctgctttaaa aatcgcaact gcctctggtg tagatgtgaa
     5221 aatcatgatt cccaacaaac cggatcatcc ttttatttat cgagcaacac aatattatgc
     5281 tcggcagctg atcaaggaaa atgtacaaat ccttgtctat gagaacggct tcctccatgc
     5341 aaaaacattg ataatggatg atgaaatctg catggtaggt tcagcaaatc aagatattcg
     5401 tagctaccga ttgaattttg aaacaagtgc tgtcatttac gatcctgagt ttttagaaga
     5461 acttgctact cagttcaaag aagatgagac acattgttca tccatgacaa ctgaaacagt
     5521 caaggaaatg tctaactggc tattattcaa gcaacaaatt tctcgattat tttctccaat
     5581 cctataattt ttttattcaa gtagagaaaa atatactaag attaaaggga ctgtgacaaa
     5641 aatcttgtca cagtcccttc ttccgaa
//
LOCUS       DANKHL010000178         5597 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000178 DANKHL010000000
VERSION     DANKHL010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.188"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..385
                     /locus_tag="PW101_002350"
     CDS             <1..385
                     /locus_tag="PW101_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HDL2514729.1"
                     /translation="IIEKDAISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVN
                     IEIVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIM
                     KKADYVTVSNDKDGVSQAFERILTI"
     gene            666..1886
                     /locus_tag="PW101_002351"
     CDS             666..1886
                     /locus_tag="PW101_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HDL2514730.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1883..4603
                     /locus_tag="PW101_002352"
     CDS             1883..4603
                     /locus_tag="PW101_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2514731.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4587..5531
                     /locus_tag="PW101_002353"
     CDS             4587..5531
                     /locus_tag="PW101_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2514732.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2080 a          899 c         1102 g         1516 t
ORIGIN      
        1 aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc ctaatcgtca
       61 attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg ttactgaatc
      121 ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg tgtttgatcg
      181 tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag atagtttaaa
      241 tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata gtcatcaaga
      301 aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag tatctcaagc
      361 atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata gaattctaaa
      421 tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt caaacgaagc
      481 gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt tttttcgaat
      541 aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac gaacaaattt
      601 tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa aggaggaaat
      661 ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca tttgaaggac
      721 tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg accgttttat
      781 caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt gcaggagata
      841 attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa cagatgaaac
      901 ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat ttctatcaaa
      961 aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc gaaacggtag
     1021 agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga tttagttatc
     1081 gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa ctcacagatt
     1141 accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac tatgctcctt
     1201 tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg catatccatg
     1261 tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct caaggtcata
     1321 ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa ggaagttgcc
     1381 aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata tcattgagga
     1441 aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca aaagtagagg
     1501 atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat acagatgttt
     1561 tggaacggat acaatcagga gagctattag actatttgtt agaggaattc tcagatagga
     1621 taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat acgaagaccc
     1681 cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag acacctcaag
     1741 atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga atattgagcg
     1801 aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt aatcaagatt
     1861 ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg cttttggaaa
     1921 atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg ggggaaacga
     1981 agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt tcccttcaaa
     2041 aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg gcaaaatatg
     2101 gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc aaaatcgcgg
     2161 aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag aaaaactgat
     2221 cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag agcagttgat
     2281 ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt taggcatcac
     2341 gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac aacaattatt
     2401 caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga aagagctgca
     2461 agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga tccaacaaga
     2521 acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg cgaacaaaca
     2581 attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat ggcaatctct
     2641 caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc tggagtcttt
     2701 ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa cactggaaaa
     2761 gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc aggagcagga
     2821 actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag aatggcaaag
     2881 attacaaatt gagactagag aaacaaattc agaattgaag attttggaac aaaggtggca
     2941 ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga agcatcttct
     3001 ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc tccaaatagc
     3061 taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga cctatccagc
     3121 attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt atatgctgat
     3181 ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga aagtgatcct
     3241 tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac caaaaattaa
     3301 aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag gacaacttga
     3361 tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg gagaagaaaa
     3421 acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag gaaagatgga
     3481 tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga aattatatgg
     3541 cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac gacaaatcga
     3601 ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa tttcagaaaa
     3661 aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg ccagaagcta
     3721 tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac aaaaagtatg
     3781 gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt cggaagtacc
     3841 tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca atgaattatc
     3901 ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat ctgtaagact
     3961 gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag caagtgaaaa
     4021 tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat tggacgaact
     4081 ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg aatggggagc
     4141 acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg aaagacaatt
     4201 accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag gaaattatca
     4261 gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag aatttcccat
     4321 ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat ttgcttacct
     4381 ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct ggcttcatta
     4441 tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag aaaagtatca
     4501 aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga tgaaccaaat
     4561 cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta atcgatgagc
     4621 agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa aacggaaaaa
     4681 aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa tactgggatg
     4741 cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta aacggtaaaa
     4801 gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta acaagggtag
     4861 atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa gaagaaatgg
     4921 aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat cgtattgttc
     4981 gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca aaaagaaacc
     5041 atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac ttagggaaat
     5101 caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt atcattttgc
     5161 atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat acgcttgcag
     5221 gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc tgtcttgcgt
     5281 tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt ttagcacacc
     5341 atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca gaaatattac
     5401 atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt cgtcaaacag
     5461 aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc tatgtaccaa
     5521 aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag acataagtct
     5581 tcgctttttt tgttttc
//
LOCUS       DANKHL010000179         5563 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000179 DANKHL010000000
VERSION     DANKHL010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.189"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(1014..1304)
                     /locus_tag="PW101_002354"
     CDS             complement(1014..1304)
                     /locus_tag="PW101_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514733.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="PW101_002355"
     CDS             1706..1942
                     /locus_tag="PW101_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514734.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="PW101_002356"
     CDS             1949..3901
                     /locus_tag="PW101_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDL2514735.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="PW101_002357"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="PW101_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDL2514736.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="PW101_002358"
     CDS             complement(4797..5489)
                     /locus_tag="PW101_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL2514737.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1809 a          970 c          902 g         1882 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttcaagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt tta
//
LOCUS       DANKHL010000180         5530 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000180 DANKHL010000000
VERSION     DANKHL010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.190"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="PW101_002359"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="PW101_002359"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HDL2514738.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="PW101_002360"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="PW101_002360"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HDL2514739.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="PW101_002361"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="PW101_002361"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HDL2514740.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="PW101_002362"
     CDS             complement(4028..5044)
                     /locus_tag="PW101_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2514741.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5146..5478)
                     /locus_tag="PW101_002363"
     CDS             complement(5146..5478)
                     /locus_tag="PW101_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514742.1"
                     /translation="MRKITMQSGLCERRIEKLTKKNSGNSMKKKKKKLLKQFAGIGNA
                     NVKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGKEMYPEYYRNALGI
                     EFKISDVQ"
BASE COUNT         1452 a         1370 c          970 g         1738 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaactt aagaaaacca
     5101 ccggagaatg aattctccgg tggacttttt gtatgattaa ccttcttatt gaacatcact
     5161 gatcttaaac tctattccaa gggcgtttct ataatattct ggatacatct ccttcccaca
     5221 tttttcacag gtaaattggg gaggattgtt cggatcattt gcaatatctg catcatctaa
     5281 aaattttact acatccattg ggattgattc tttttcacca caacttagac atataaattt
     5341 cacgttggcg ttcccaatcc cagcgaattg ctttaaaagt tttttctttt tcttcttcat
     5401 actgttgcct gaattctttt tggtcaattt ttcgattctc ctctcgcata aaccgctttg
     5461 catggttatc tttcgcatat ttaataacta accgtccatt tttgcgcaca ctcatcccaa
     5521 cgaattcata
//
LOCUS       DANKHL010000181         5527 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000181 DANKHL010000000
VERSION     DANKHL010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.191"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            27..845
                     /gene="purR"
                     /locus_tag="PW101_002364"
     CDS             27..845
                     /gene="purR"
                     /locus_tag="PW101_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDL2514743.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1348..2721
                     /gene="glmU"
                     /locus_tag="PW101_002365"
     CDS             1348..2721
                     /gene="glmU"
                     /locus_tag="PW101_002365"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDL2514744.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2815..3786
                     /locus_tag="PW101_002366"
     CDS             2815..3786
                     /locus_tag="PW101_002366"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL2514745.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3792..3894
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3960..4529
                     /gene="thiT"
                     /locus_tag="PW101_002367"
     CDS             3960..4529
                     /gene="thiT"
                     /locus_tag="PW101_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HDL2514746.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4610..5383)
                     /locus_tag="PW101_002368"
     CDS             complement(4610..5383)
                     /locus_tag="PW101_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514747.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
BASE COUNT         1805 a          977 c         1162 g         1583 t
ORIGIN      
        1 aaatttctta aaaggagttg gaataggtga aagtacgtcg cagtgaacgt ctagttgaca
       61 tgacacagta tttattgaat cacccgcatg aattgatttc cttaacaagc tttgccgaac
      121 gttatgaatc tgctaaatct tctatcagtg aagatttggg aatcatcaag aaaacattta
      181 aagaacgagg gtatggaacc ttggaaacaa tcccgggtgc tgcaggtggt gtattgttca
      241 tccctgaaat ttcatatgaa gaggcgaaaa aatatattga atctctagca gaacgtctat
      301 cagaacaaga ccgtctgctt ccaggaggat atgtttatct ctctgatttg ttaggagaac
      361 ctgaactgct gcgtcaagtt ggaagaatca ttgcttctaa ataccttggt gaaaaaatcg
      421 atgctgtaat gactgtagcc acaaaaggcg tacctattgc ccaagctgtg tcctattatc
      481 tgaatgcgcc attcgttatt gtacgccggg actcaaaaat aacagaagga tcaacagtaa
      541 gtgtcaacta tgtgtctgga tcttctgaaa gaattgaaaa gatggaactg tctaagagaa
      601 gtttgaaacg tggttcacgt gtccttgtag tggatgattt catgaaaggc ggcggaacag
      661 tcaacggaat gaagagcttg attgaagaat tcgaatcaga acttgttggc atcactgtat
      721 ttgccgaatc aaaattcaac ggtcatcgcg ccatcgatga ttatacgtca ctgctttatg
      781 tcaaagatgt agatactcaa acgaaaaata ttacggttgt gccaggaaat tatttttcag
      841 aataagaggt tgtgaaaaag ctgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
      901 gctttccaca ttttttcata aatttggctt aataccgaga aactggcttt tgaacaccgt
      961 ttatctagag gttgtgaaat cagcgtcctg tcctgagtat tagctcaata aacggaaaaa
     1021 ttgctcctca tattttgccg tttattgagc taataccgaa ggtcagctga ttgaaaaacg
     1081 tttaatgaag aggttgtaag cctaccgttt aactctaagt cacaagaaac gatttcaaaa
     1141 aacagctttt catgttttat gagtagtgtg acttgtgacc gaggagttgg caggagagca
     1201 ccggttacca aatagatagg cccgttatgg tctatctatt tttttatcca gaaaacgctg
     1261 ggataaaaga aaatcacttg ttttgttgaa aaaatgggta tactgggata aactgaaatc
     1321 agcaaactat atttaggagt gacgcagttg gacgcacgtt atgcaattat tttggcggca
     1381 gggaaaggta cccgcatgaa gtcaaaatta tataaagttt tacatcccgt ttctggtaaa
     1441 cctatggtag aacatatcat taatcgggtc agtgaaacga aaccagatga agtgattacg
     1501 atcgttggac acggagcaga acaagttaaa gcacaactag gagaacgcag taaatatgcg
     1561 ttacaagcag aacaattggg aacaggacat gctgttttgc aagctgcttc tttcttggaa
     1621 ggaaaaaaag gaacaacgtt agttatcagt ggcgatacac cattattgac tactgaaacg
     1681 ctgaacaatc tttttgaata tcaccaagga aaaaatgcaa gtgcaacgat cttgacggca
     1741 caagcagaaa acccaacagg atatggacga atcatccgtg accatattgg gatcgtagaa
     1801 aaaatcgtgg aacaaaaaga tgcaacacca gaagaagctc ttgtacaaga aatcaataca
     1861 ggaacttact gttttgacaa cgaagctttg ttcgatgcgt taagcaaagt tggaacaaac
     1921 aatgctcaag gtgaatacta tctaacagat atcatcgaaa tcttaaaaga agaaggccat
     1981 acagttgctg cttatcaaac ggatgatttt gaagaatcaa tgggagtcaa tgatcggatc
     2041 gcgttagcta aagcaaatga gatcatgaga aagcgaatta accagatgca tatggtcaat
     2101 ggcgtaagct ttgtcgattc agcaacgaca tacatcgatg caggcgtaga aatcggtcca
     2161 gacacattga tcgaagcagg tgtacaaatc caaggaaata cagtcattgg ttctgattgt
     2221 gtcatcgggt cacattcaaa aatcgtagac agtaggattg aggatcacgt cgtcattgaa
     2281 aactcagtca ttgaaagcag ccatgtcaag aaacatgcag atgtgggacc ttatgcacat
     2341 ttacgaccaa aagctgaaat tggcgaaaac gtccatatcg gaaactttgt ggaagtgaaa
     2401 aatgctcaaa tcggcaaagg aactaaagtc ggtcacttga cttatgttgg tgatgctacg
     2461 ctgggagaag aaatcaatgt cggatgtggt gttgtcttcg tcaactatga tggaaagaac
     2521 aaacatcata cgactgtcgg cgaccacagc tttattggtt ctagcacgaa tatcatcgga
     2581 cctgtcgagg tagctaaaaa cagttcgatt gctgccgggt caacaattac agacaatatt
     2641 ccagaatatg cgctggcaat tgcgagagca agacaagtca ataaagaagg atatgcaaaa
     2701 aaactccctt atctaaacta agacttttgc aaaatcactt gatttattta aaaaatatga
     2761 ctactattta ttaggtagaa ttctagagtt gtgaaaaaga aagtggaggt ccccatgtca
     2821 aaacattact ttgacccaag attaaaaatt tttgcgttaa attccaatcg cccattagca
     2881 gagaaaattg cagcagcagt cggcgtcgag ttaggaaaat catctgttac tcaatttagt
     2941 gatggagaaa ttcaagtaaa cattgaagaa agtattcgtg gatcgcatgt ctatgttatt
     3001 caatcaacga gcagcccagt caatgataac ttgatggagt tgctgatcat gattgacgca
     3061 ctgaaacgag caagtgcaaa aacaatcaac gtagtcatgc cttattatgg ctatgcgcgt
     3121 caagaccgta aagcacgtgc acgggaacca atcacagcga aattagtagc aaacatgatt
     3181 caaaaagctg gtgcaacaag aatgctgact ttggaccttc atgcagttca aatccaaggt
     3241 ttctttgata ttccagtcga tcatttgatg ggtgcgccac ttattgccaa ttatttctta
     3301 gaaaaaggaa tcaaaggcga tgatgttgtc gttgtttcac cagaccatgg tggtgtcaca
     3361 cgggcacgta aattagcgga atttttgaaa tctccgattg cgatcataga caaacgccgt
     3421 ccaaaagcaa atgttgcaga agtaatgaat atcattggac aagtcaaagg caaaacttgt
     3481 gtactgatcg atgatatgat cgatacagca ggtacgatca cattggctgc caatgctttg
     3541 aaagaagctg gtgcaactag tgtgtatgct tcatgtacac atccagtatt atccggccct
     3601 gctttacaaa gaatagaaga ttcagctatc gagcgtctag ttgttacaga ctcgatctat
     3661 cttccagaag atcgtaaaat cgacaaaatc gatgaagtaa gtgttggcgg actaatggga
     3721 gatgcaatca aacgtatcca tgaaaacaaa ccagttagtc cattgtttga aacaaaaaat
     3781 aaataataaa taaactcaca aaggggagtc caattgggct gagattgaat ctatttctaa
     3841 acccttcgta cctgtatcgg ttatgcgagc gtaggaattg tgaataaaca gctgtttgac
     3901 tattttcgaa agctgcttcc tcctttgtga cttttttatt caaaggagga agttttttta
     3961 tgggaaaaaa tagagtttgg gtagaaggaa cgattgttgc agcgttagcg atggtgttgt
     4021 cgttgattcc gatccagatc ggcagcagtt tttctatttc attagggcag attccattga
     4081 ctctttttgc gttaagaaga gggacaaagg cagggatgct ggcaggtttc atttggggca
     4141 tcctacattt tccgttggga caagtttatt atttaagtgt cgttcaagtg ttgattgagt
     4201 acccgattgc ctatactttt gcaggcgcag cagggctcat ggcttcaaat gtccaaaaag
     4261 gctatgtaga cgataatacg aaacaaatta gaaaaagcat cgtgtttggt gcattgattg
     4321 gcgcactttc cagatatttt tggcatttta ttgcaggagt ggttttctgg ggagcgtatg
     4381 cactatgggg gatgaatcct tggctctttt ctttcgtaat gaatggtgcc agcgggttga
     4441 gtacagctat tgtaacgatc atcgttttat tagggatcca acaaacgact ccttcgattt
     4501 ttgtgccaga taaacgacag gagctgtaaa taaaaaagaa gcaagaaact tgagacagaa
     4561 tcatgtccta gtttcttgtt tcctttttat tttttgataa gaaatagatc tacttcaatg
     4621 gcagataaga aagatcggca tcttctacca attgccagtg tcgatcggtg acagagtaga
     4681 ttaaagtagc gatgatttcc tcttctcgat accgggtgcc ttttaatttc caaatataat
     4741 cttcaatcac atagattggg atacgatatt taccatcgat caaaccaact tgatcaaaag
     4801 aaaaaatgat cccttcctgc atcaaattac gcatttcttt caataattcc acactgattt
     4861 tttcaacagg tgtttctttt ttgaaaaaag atttttttac attgatctta tctgacaaga
     4921 tcgaacttaa tttactagca aaaatcctat aaaattcatc taaatagcga taataaatcc
     4981 gattcagctg atcattaaga tcgaagtata caaaattatt ctgtagttta taaaaaaacg
     5041 gcgaatgaag atgtgttttc atatgaccaa aatataataa ttctgaaatt tctaaaggtg
     5101 tcaattcttt cagcatcgtt aaatcagtaa agtcgatcca cttcaattca tcgaatccac
     5161 gcctgttacg agaagaggaa aagaaacgct gaatattttc tgtaccacgg attactttca
     5221 gccctgtatg ggattcatat tctccagcag aagcagatgg atcaagcaag agcaagtttt
     5281 gaggaaaatg cacgatactg tttgcaaagt cagtagcagt cagccctttt gttaaaacag
     5341 cattacttgt ggtatcaata tgtacataaa ctgactcagt catcatgttc ctcctcttat
     5401 ttcctcttaa tctcttttat tttacaacat ttgcattaaa aaagcttgtt tcttttattt
     5461 taaaattcca ttacaaaatc acgttttcat tgaaaacact ctcattttca attaattttt
     5521 gtgattt
//
LOCUS       DANKHL010000182         5506 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000182 DANKHL010000000
VERSION     DANKHL010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.192"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            81..2140
                     /locus_tag="PW101_002369"
                     /pseudo
     CDS             81..2140
                     /locus_tag="PW101_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
     gene            2223..2792
                     /locus_tag="PW101_002370"
     CDS             2223..2792
                     /locus_tag="PW101_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2514748.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2967..3773)
                     /locus_tag="PW101_002371"
     CDS             complement(2967..3773)
                     /locus_tag="PW101_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514749.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3911..5425
                     /locus_tag="PW101_002372"
     CDS             3911..5425
                     /locus_tag="PW101_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HDL2514750.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         1954 a          954 c         1119 g         1479 t
ORIGIN      
        1 tgattttatt tatataatga aatcatctta tgaaaattac tacttcatgt ttagacaaga
       61 gtagggagga ctaagaataa atgtgttcaa aaagacgttc aaaacaacta aataaacacg
      121 cttgtcgttt agataaggaa cggtcgtgtt ctgaaaaaca ctacgttaag tttatgatgc
      181 gcacgatgct gatcagttta tctgtaggtg tctatatgca tgtacccgtt caggcaacga
      241 cacaaaccat caaggtcgta cgtacctctg tttctgagac cactgattcc cctatcacta
      301 ttactcatac tttcactgat gcaaatggaa aacagctact tcctgcattg acttcctata
      361 ataaagtcag ttatcctcca acgataaacc actactggtt aattcctaac aaaacattat
      421 ggacatcgga aaccggaagc agtgtgccgc tttctatctt ttccgatatg actgatccta
      481 caaaaggtga tcagttaaaa cagatcactg atattttgaa tcatgctttg acagatataa
      541 aggacggtgg aagcgcacaa gtttcttatg tatatgaaaa agattattca cagtttgaaa
      601 gaaagaaaat cgatgttctg gcagggacaa aagtttcgat atctgatctg gtagaaaggc
      661 tgaagaacgc agatggaaca caaggagata tgtccaaagt aatttcaaac aatgggaata
      721 tcgtggatac gactaataca ggaacatcta ctatttcctt gtcctatttt gatccaattg
      781 ctttaaaaac aatgactgca tctgctgtga tccatgttcg tgctgatcaa acagctatca
      841 aaggaaaaga tcttcactta agaacaggag agagttacgc ccctaaagat cttgttgaat
      901 atgtaacaaa aagcgatgga acttcaggaa ataaagaaga aatcaaaatg aacgggacag
      961 tagatactac aactccggga gaatatcccg ttgtattaac ttatatagac tcgaaaacat
     1021 caaatgtggc agaaacaaca gctgtagttt atgtagaagc atctgctgct tcatcgactg
     1081 tcatcactca ttttatagat agtcaaacaa ataaagaact tcaagcagaa acacatatga
     1141 gtagtgtaaa tcgtattcct gatttgcgcc tacctgctga attttctgat cgagtgccaa
     1201 atatagattt atcgacttat gaacaaaatg ggaaagtcac ttctatttct gattggatgc
     1261 aagcaaacgg attagacaga aaaagtcact ggtcggagat ccttgaagta cttcactcag
     1321 aactgatgaa taataggaag caatcaggag gggaaattac ctttacttat gtttatatac
     1381 cagacaatcg ttcattagaa ggtcatgatc ttattgttca tccaaacgaa aaaataaaca
     1441 aagaagagtt gatccaatca gcaacaaatt ccttaggtaa acccgtaccg ttaaaggatg
     1501 tatcggttac aatcaatgaa gtaccattgg aggatggtta tttgaccaaa caaacaggtg
     1561 attttacgat tatgtatagt tatcatgacc cgtattcact ccaagaactc catgctcagt
     1621 cactattaca tgttttacca ttaaacaaac agatagatca aaaagaacaa aaaagacaag
     1681 aagaaagctt gtcagaaaaa aacaacaaat ggctcatgca gtttctccaa cagggaaagt
     1741 aaaacaaaca agaagcctag agttagcaag aatatctaga aaaatctcag aatctaataa
     1801 aacgataagg caagaagctc atttggcccc acaagtttct ggattggaaa atgaaatttt
     1861 agagcctaaa ataaaacaac aatcagaaaa agaatttgtt gctaaacagt cggaagatca
     1921 gaaggaatca gatgaccaga aggaaacgaa gaaaaaacgc ttgtcaacta ttgattccac
     1981 cgatgagcat ctgcagccgc aagtaccact tcctagtccg ggaacacccg cacctggtgg
     2041 aggtattcct tcttctgctt taggagattt catgcgaggt atggcaggaa catgggtata
     2101 cgctaatcga aatgaatttg acgcagatga ctggttctga tttatatctc tatctgttat
     2161 agtcaaataa gtagctgagt catatgttat ttggcataat ggagacgagg agggatagaa
     2221 aaatgttaga taaaacgtta ccttacgcag aaatctggat gacaagacca cttcaacaaa
     2281 atgttccaat cacgccttta gcggaaggtt ttgaatttcg attttatcaa aaagaagatg
     2341 aatctgcttg ggcggagatt gagacttccg ttggggaatt tcaaacgaca gatgaagcac
     2401 ttgagtattt tcaacaaaac tttgctcctt atcctgaaga acttgaaaga aggatgtttt
     2461 ttgtagagac aaaaaaagga gaaaaagtag caacctgtac cgcttggcga aaaaaatcaa
     2521 gagagcaagt gtatccggtt tttcattggt tagcagtgaa accagattac caaggaaaag
     2581 ggctcgcaaa agcattgact gccagagtct tgcaagaatt tcctaccctc catacggacg
     2641 gaccaatcta tcttcacaca cagacatgga gccatcaggc gattgctctt tataaaaaaa
     2701 tgggttttac ttttattgct gaaaatctag acggcacacc taatccagat tatgaaaaag
     2761 ttatgcaggt tttaagtaaa ttagataaat aacgaaatgg tgaaataagt cctcagtatt
     2821 gggataaaaa acgaaatagt cattcctcat atcttacttt tttgagttaa tactatcgga
     2881 taactaatgg catgccatac attcaaaaaa gaaggggcag tgacaaaagt attgtcactg
     2941 ccccttctct tgttttttag catgttttat cgtttgattt ttttaggaat aaaattcttg
     3001 atgaacgtaa atttttggta atcgtttcga ctatagaggt aaccaaatac actgaaaaca
     3061 actgctccaa taaaattaac aaataaatcc ttcatagtat ccatgattcc cacgtctaag
     3121 taaccatttt ggatgattgt tttatctgtt ttaccatctg cattttgact ttcaatgatt
     3181 gtccgagtga tatctggaac atgatagaca gtgtttccgt ctttacttaa ctccacacta
     3241 ttgatttgct ggatgatcgt atctttttgc atatcgaatt gcagtaattt atctgctgaa
     3301 aattccacaa actcccacat aacaccaact gtcatcgaaa aacagaagga aacaactgct
     3361 agtaataaag gagtcaagtt catggttttt gtatttttat tgagcaaata aacaagggaa
     3421 aagccgacac ccccagctaa aaatccgctg aatgcatgta gaaaagtatc ccacattgaa
     3481 aaatagccat aaaaatcact taactctcct aaaactgttg aactaaaaat aaaagataac
     3541 aaaatgattt ctagcatatt tggaatctgt atctttaata tcttttccag aaaataaggg
     3601 atttcaaata aaattaatgt cagtaataaa agcattgtat ttccccagtg ttggttccag
     3661 acctgatcca ctagcataat aacgacgatg atacgtaata aaatatagat tccgtttgtg
     3721 attctacctg cagtttttgt actgttcgta aaaaaggttt tcaaatgatt cataaaataa
     3781 ctccttcatg tatatcctta attatactaa gaagcgtgtg tttaccaaag ctatatgact
     3841 taaagaaaaa cagagttgat attgtgaaaa aaatcactta atatagtata aggagaaaag
     3901 ggggatacaa atgaaaaaat tagttgctgg tttattaaca gccgttgcac ttcttgctat
     3961 gagcgcatgc ggcgcagatc agaaaaatac agcgcagaca agttctagta cgaaggaatc
     4021 tgccgaagta tcatcaggcg cttctgaaca agtatataca gatccaaaag aaatgaaaga
     4081 atcttatgat gtcgtgatta ttggagctgg tggtgcaggg atggcagcag ctatcgaagc
     4141 aaaagatgct gggttgaatc cagtaatttt ggaaaagatg cctgttgccg gaggaaacac
     4201 atctaaatca tcaagcggta tgaatgcttc agaaacaaaa tttcaaaaag aagaaggaat
     4261 caaagactcg aatgatgcct tctatgaaga aacgctaaaa ggtggtggag gaacgaatga
     4321 taaagaaatg cttcgatact ttgtcgatca ctctgctgat gcggtcgatt ggctagatca
     4381 aaacggtatc aaactggaca acttaacgat cactggaggg atgagtgaaa agaggacaca
     4441 tcgtccgtct gatggctcgg ctattggtgg atacttagtg gaggggttat tgaataacgt
     4501 ccatgagcgg gaaatcccta tttttgtcaa cgcagatgtg acagacatta agaaaaatgg
     4561 ggaaacggtc aatgaagtga ctgtacatgt tcaaggagaa gaaccaaaag aggtcaccgg
     4621 aaaagcaatc gtagttacca caggcggttt tggtgccagc aaagaattta ttgaaaaata
     4681 tcgtcccgat ttagctgatt atattacgac aaatcaagaa ggatcaactg gagatggtat
     4741 caccatgatt gaaaaattag gtggacaaac cgttgatatg gataaaatac aaattcatcc
     4801 aactgtgcag caggatgagg gggtcttgat tggtgaggtt gttcgcggag aaggagcaat
     4861 cttggtcgat caagagggga aaagatttgt caatgaaatg gatacaaggg ataaagtatc
     4921 tgcagcaatt acagcattgc ctgaaaaatc agcttatttg atttttgacc aaggcgtgcg
     4981 tgatcgtgcc acagctattg aattttatga ccaaaaagga tatgtcacaa ctggaaagac
     5041 aatggaagaa ttagcagaaa aaatcgattt accaaaagaa acactagcaa aaacagttga
     5101 tggatggaat acggatgttg cggagaaaaa agatgctcaa ttcaaccgtg ccacagccat
     5161 ggaacatgac ttatcgtctc cgaattatta tgcgatcaaa atcgcaccag gaatccatta
     5221 cacgatgggc ggtgtgaaaa tcaatacgaa cactgaagta ttagataaag aaaaccagcc
     5281 aattaggggg ttatatgctg ctggtgaagt cacaggtggc ttacatggaa acaaccggat
     5341 cggtggaaat tcagttgcgg agatcattat ttttgggcga caagcgggca tacaagcagc
     5401 tcgatttgct tcggaaaatg aatgaaatag aacgtaaata aaaagaggtt gtgaaatcag
     5461 cgttttgagc taataccgag ggtcagctga ttgaacactg tttagt
//
LOCUS       DANKHL010000183         5475 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000183 DANKHL010000000
VERSION     DANKHL010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.193"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            18..884
                     /locus_tag="PW101_002373"
     CDS             18..884
                     /locus_tag="PW101_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2514751.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(906..1343)
                     /gene="ndk"
                     /locus_tag="PW101_002374"
     CDS             complement(906..1343)
                     /gene="ndk"
                     /locus_tag="PW101_002374"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDL2514752.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            2002..4680
                     /gene="mgtA"
                     /locus_tag="PW101_002375"
     CDS             2002..4680
                     /gene="mgtA"
                     /locus_tag="PW101_002375"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDL2514753.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            4767..5438
                     /locus_tag="PW101_002376"
     CDS             4767..5438
                     /locus_tag="PW101_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2514754.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
BASE COUNT         1798 a          925 c         1186 g         1566 t
ORIGIN      
        1 taaaaaggag aactattatg tatgatggtg aattgatcaa gaaacttcga gtcaacaaga
       61 agttgacaca gtcccaatta gcggaaggaa tctgttttaa gacttctcta gtcgggattg
      121 agacaaattc agtgaaaaaa atgtcatttg taacattgag atcattttta gagcgaatga
      181 acatgacact tgcagaatat gaatggatgc gaaaccagct ggatgaacca ataaagttaa
      241 agaaaaaaag acgaatgcta gatcgggtac aagaagacag ctttgatcct tataaagaga
      301 tcagcaataa ccgtaggcat tttaagaaaa cagaagatct gtattatttg gttttgaatc
      361 ttcacatggt cgaaaaaaca gaagtatctg agacagaatt tgaattttta cgttatgaat
      421 gccgaatcat tgaagaatac ttgatgcaga taccagacta tggtcaattt gaactatcta
      481 ttctagctga ttttccttgg atattcagtg atcggttcat tgagaacaat tatttgaaaa
      541 tcaaaaaaag aatgcgacga ttagcacagt tgaacaatca tgagcagtat ctctttactt
      601 ttttaatgaa cctgacttcg ttttatatag aaaatggtcg gttgaaaaag gcacgtagtg
      661 tgaatgaaga tatgaaacgg agcttagcac ataaagaaag aagtacagtg atctatgaaa
      721 cattgatggc agattattat caaagattga tttctgctgc tttaggggaa gaaaagacga
      781 aagactacca agaattcttc acagtgctcg aatatatgtt aggaaagaat caacggaggt
      841 ccttagagaa aaaactgctg gccattgctg aacccagcaa ctagaccagc agtttagaat
      901 ttgtatcact tcgccgcttt ttcagacggt ttgtttatat cgaatggcgg gaaaaagcgg
      961 gcgatctctt ttaccgcagc atcacgggaa tcagaagcgt gaatgatgtt ttcagtcccc
     1021 ggcagcgcgt agtctcctcg gatcgtacct ggaacagcat cggcagcttt tgttgctcca
     1081 accatcttac ggacgatatc gatcacttcc tcgccgacca gcacaagata aacgacagga
     1141 ccggaagtca tgtaatcgat cagttcatca aaaaaactgc gctcagtcaa gtgctgataa
     1201 tgttcttttg ctaattcagg agtcatagtt tcaaacttca tttcagcaat ggccagtccc
     1261 tttgcttcaa aacgttggat gatgcttccg actaaatggc gtctcacgcc gtctggcttg
     1321 ataataacaa gtgttctttc cataacagca cctcgtttcc tactatattt ctattatagc
     1381 gggaggtgga aaatgagaca aataaaaccg cgtgagacag gaatataacg tgtgtatttt
     1441 aaaaaaagta tgaaaaacat ggaaaattga tttgacatcc tcttttttag ttgttatatt
     1501 aacgaaggaa aaccgaataa gatttgttag gtgaggctcc tatacaaaca taggctactg
     1561 cccaaaaacg tcgagagacg ccaatgggta gaacaggaat tgtcgagata aggcttttca
     1621 taaggtagct aaaagcaggt atctgctttt tacgttgtac agtgtcaaaa ctcaacgatg
     1681 agatctgaga agcttttttt aatatgcttt ttcaaacctt tcgctgagta tttggcgaga
     1741 ggtttttttg ttgccgcaaa tccccatcgg tgaaaaataa aaaggaggtg gtctaattgg
     1801 aaaaataact attcgaaaaa tgattggaag tcatgaatta aaattactta gtaaaggaga
     1861 tgagaacaaa tggatgacag tcattcgaaa aacaaggatc tagaggaaag gccagggcta
     1921 ttatctatct gttctcagcc cctttcagtt ttttctctat cgatccgatg aatagctaaa
     1981 gaccaaaagc agcgagaaca gctgtctttt ggacacaaag gagagattga gatggaaaaa
     2041 aagaaagata cacaaaaaat ctatgcagct tttgctggaa aaacgaaaca agaattattt
     2101 gaactattcc atgcatcagc aaatggattg acagaagaac aaatcgaacg ttcaagagaa
     2161 aattacggtg aaaatgcgat ttcatatggc aaaaaaacac cattcattgt agaaatattg
     2221 aaagcataca tcacgccatt tacattagtg ttgatcgcat taggaattat ttcattcatc
     2281 acggaatatg tattagcagc tcctggagat aaagatcttt tcggtgtagc cattatcttc
     2341 actatggtta tcgtcagcgg gacaatgacg ttgattcagt ccgtacgttc caatcaagcg
     2401 gctgaaaaac tgaaatcact tgtgaaagta acggcagctg taaaacgtaa tggaaagtac
     2461 acagagattc ctatggaaga aatcgtctgt ggagatcttg ttcggctttc agcaggggat
     2521 atgataccag cggatatgcg tttattgtcc gcaaaggatt tattcatttc ccaagcagca
     2581 ttgacagggg aaagctatcc agtagaaaag catgcgaaag tatgtgaaga cgatcagcaa
     2641 agcgaaacaa gttacgaaaa tcttgtcttt atgggaagta atgtcatcag tggaagtgcg
     2701 gaaggaatca ttgtttctgt tggaaatgaa acactatttg gtcatgtagc agaaacgcta
     2761 tctgaaaaac caattcaatc aagctttgaa ttaggcatcc ataaaacatc catgctactg
     2821 atcaaattta tgtcattgat ggctccagca gttatcgtca tcaatgggct tacaaaaggg
     2881 gattggctag aagcttttct ttttggacta tctgtagcag ttggtctaac acctgaaatg
     2941 cttccaatga tcgttacgac gaatttggtc aaaggagcaa gtgtgatggc caaaaaagga
     3001 acagtgatca aaaatttgaa tgccatccag aactttggtg caatcgatgt gttatgtacg
     3061 gataaaacgg ggacattgac acaagataaa atcattcttg aatatcatat ggattgtgaa
     3121 ggaaatgaag atgatcgtgt tttgcgccat gcgtatctaa atagctacta tcaaacaggg
     3181 ctgaaaaatt tgttagatgt ggctatcatt gatgaagcaa cagaaacttt agatacagat
     3241 aagatcaatt atcaaaaagt ggatgaaatt ccttttgatt ttgaacgtcg tcgtatgagt
     3301 gttgtggttg ctgatccagc tggtaaaaca caaatgatca caaaaggtgc gattgaagaa
     3361 atgctgaaca tttctaaatt tattgatctt ggcggtactg ttacaccatt aacaaaagaa
     3421 cgaaaagaag ccgtacttgc aaaagtacag tcacttaatg aagatggctt acgagtgatt
     3481 ggtgtcgcac aaaagaccaa tccaagcgtc gttggagaat tttcagtcaa agatgagtca
     3541 gacatggttt tgattggtta tcttgctttc cttgatccac caaaagaaac aacgaaacaa
     3601 gcgttgaaag ctctgaaaga acatggtgta gctgtaaaag ttctgactgg ggacaacgaa
     3661 ttagttacac gttcagtgtg ccgtcaagta ggtctagaga tcaatgaatt ggttaccggt
     3721 gagaaaatct cggaaatgag tgatcatgaa ttagcacaag tagctgaaga ccatgaagta
     3781 ttcgtaaaat tgaatcctca gcaaaaagca cgtttgacga cagctttacg taaaaatggt
     3841 catactgtcg gatttttagg agatggtatc aatgacgctc ctgcaatgaa agtggctgat
     3901 atcggtattt ctgtagatac agctgtggat attgcgaaag aatctgcaga cgtgatcctt
     3961 ttagaaaaag atttgacgat tttggaaaga ggccttcttt ctggacgaga aacttttggg
     4021 aatatcatga aatatatcaa ggctacagca agttctaatt ttggcaatat gttttccgtg
     4081 ctgattgcaa gtacgttcct gccattttta ccaatgctgc ctttacaact tttgtttttg
     4141 aatctgatct atgatgtttc ctgcatttcc ttgccatggg atcggatgga taaagaatat
     4201 ttggacgagc caaaaaaatg gaatgcatct agtattgggg aattcatggt ttactttggt
     4261 ccaacaagtt cgatatttga tattactact tacttgttga tgtattttgt tatttgtcct
     4321 gctgtagtgg gcggaagttt ccatacattg gatagttcac agcgagtgat tttcatcgca
     4381 ttattccatg caggatggtt tgtcgaatca ctatggacac aaaccttagt gctccatgca
     4441 ctgcgtacac cgaaagtacc atttatccaa agcaacgcaa catttgccat gtttgttatc
     4501 actacattag gaattattgt cggttctgtc cttcctttta caagttttgg tgctgaatta
     4561 ggcttgatgc cacttcctgg caattactgg ttatggttat ttgctacagt catcgcttat
     4621 ctggcgcttg tgacagctgt gaagaaaatc tatatccgtc gattccaaga actcttataa
     4681 acgagaaggc tggttctttg cttctgttct ttttggacca gtcgtgctaa actatgtgta
     4741 ttgttttgga aaggattaat gtatagatga aaggtgcaat atttgattta gatggtttgt
     4801 tagttgacac agaaaaaacg tatcgtgatg gctggctttg gggatttcag caatttgata
     4861 tccatatccc aaaagaaatc gttgacgctt ggggcggaaa aaactggaaa caaagttttg
     4921 atattttagt gaaagcagca ggtagccctg aaaaagtaca ggaagtccga gcgaagcgtg
     4981 aagaatattt ttatcaacag ctgacaagcg gagccatcca cttaaaacca tatgcaaaag
     5041 aaatattggc agacttaaaa gaacgtcata tgaaactagg cattgctacg accactgtta
     5101 cgaaaagagc aacagatatc ttggatcact ttaaattagc tgattacttg gacacgttta
     5161 cattcggaga cgaagtgtcg gagaacaagc cttcaccgat tccttatctg acagctttgg
     5221 aacgaacagg attagaagct agttcagcct ttgcagtgga agattcttta gtaggagcaa
     5281 cagctgcttc acgagcagga ttaggcgttg ttttgatacc tgattccagt tttgtccgaa
     5341 attataccgt cgaagaaaag gaaaaattga aacttcttgc ggaaggaaaa gatcttcgaa
     5401 ctgttattga aatgttaaat aaaaaaacag aaaaataaat atagaaaaaa taatttgaat
     5461 ttcgtacttg acttt
//
LOCUS       DANKHL010000184         5417 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000184 DANKHL010000000
VERSION     DANKHL010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.194"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(118..735)
                     /locus_tag="PW101_002377"
     CDS             complement(118..735)
                     /locus_tag="PW101_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514755.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            complement(797..1477)
                     /locus_tag="PW101_002378"
                     /pseudo
     CDS             complement(797..1477)
                     /locus_tag="PW101_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(1799..2020)
                     /locus_tag="PW101_002379"
     CDS             complement(1799..2020)
                     /locus_tag="PW101_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514756.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(2118..2363)
                     /locus_tag="PW101_002380"
     CDS             complement(2118..2363)
                     /locus_tag="PW101_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514757.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            2603..3217
                     /locus_tag="PW101_002381"
                     /pseudo
     CDS             2603..3217
                     /locus_tag="PW101_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(3587..4588)
                     /locus_tag="PW101_002382"
     CDS             complement(3587..4588)
                     /locus_tag="PW101_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HDL2514758.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(4747..5013)
                     /locus_tag="PW101_002383"
     CDS             complement(4747..5013)
                     /locus_tag="PW101_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2514759.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(5003..5359)
                     /locus_tag="PW101_002384"
     CDS             complement(5003..5359)
                     /locus_tag="PW101_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2514760.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1675 a         1004 c          747 g         1991 t
ORIGIN      
        1 tgctcttttc acttttcgga ataaattcac tgtgactctt gataccttct tttatatatt
       61 gctaatgtat tttctccatt gctatgaaaa tagaatcaac atatttataa tatctatcta
      121 atctattttc atttcaccgc agcatcttat taaataacgt gtaaataaga atataaaagt
      181 tattgaacta attaataaag atagaatcat cgtagttgga tacgtagatg aaacctcaac
      241 tatagttctt ttccccgtac ctttaataga cactaaacca ttgtcatttt ctatagtgct
      301 ctttttccca tttactttca caactgttcc ccaataattg aatacaggta atgtgatatt
      361 ttgctcatcg gcatcttcta ctgtatagac tattttggaa ttatatgctt tatgagatgc
      421 attctcccac cgatcaccaa cttttacttc atgtttcttt aaatgatctt tatatttcaa
      481 tgcatctaat ggtgcatagt catataggcc tccagttctt gaggatgtat aacgtgtaac
      541 atcttttttg gtcataacta aacgaccttt atacgactga attttatgcg aataatttgc
      601 ttctgttgct gcagtcacag taaaaataat aagaactatt cctacaaggc tccacatact
      661 ttttttgttg cttgttttcc actttaaaaa cactatggtt aagattaatg aaccaaaaag
      721 cacttgaaaa cccaaggttc tcggttctgt tgcgaagttt gaaaatcaaa cgcgccctct
      781 ctgaactcca aatatcttag gctgttattc ccattaatac cttgatttca gtagacaccg
      841 aaaagccgaa gagcgttcca tttcttcggt tctttttata tattcctcga atggtctcca
      901 tgcccttaat cgtggaagag gctgtacgga gactttgata aaatttattt cgtcgtttaa
      961 taggtcgatg gtcttgttct attaaattgt taagatactt cacagttcgg tgctctgtct
     1021 tagtatataa acccacactc tgtaactttc taaaggcgga gccaagagaa ggtgctttat
     1081 cggtcacaat tgctttcggc tcaccaaact gtttatggag tcgttttaag aaagcatagg
     1141 ctgcttgcgt atcccgtttc tttcgtaacc agatatctaa ggttaagccg tccgcatcaa
     1201 ttgcacgata aagataatgc caacgtccct taattttgat ataggtttcg tccattttcc
     1261 atgaatagaa ggattgtcta tttttcttct tccaaagata atagaggact ttgctgtact
     1321 cttataccca acgataaatc gtagtatgac aaacatttat tccacgatca tataacaatt
     1381 cctgaacttc acgatagctt agattgtaac gcaggtagta accaacagcg acaataatga
     1441 cgtctttttt gaattgtttg cctttaaaat gattcattac tctgtcctct ctgtcttttt
     1501 tctcaatttt acactaaaat agattttttg gaaaactttg caacagaacc gtttttacta
     1561 tatacttctt cccagaactt gtccgtctcc ttcaaattat ctctacctct cgagttcatt
     1621 gggtggtttg cccatttatt aaacatggaa tcacctttct catactcata tatttatgtc
     1681 ctatttcaat tatatttata tatgcttaaa taaatttgta tcctgaaatt tattgtaata
     1741 tgaagctgta ttttacatat agtaaccaaa gagttaataa aataaaaaga gcgaaccatt
     1801 atagatcagc tcttttttca ttgctgtttt taatcttatc tagaatcact tcaaatgttt
     1861 caaaggtatt tacatctaac tgacataacc aaagcaagta cttcttttct ttgtaagtcc
     1921 aatcaatatt acggtaagag gacattagat tatggtacct tgctctttgt tcaactgaag
     1981 ccaattccca gcgtttttca aatatttttt cttcttccat tattgatccc tcgcaagacg
     2041 ctacctcaaa cttaaataat gggcagcgaa agtctaattc cttagatcta agtatagcaa
     2101 acaaatatga aaaaaactta gaaggtagta aaagttcttc cgtcttttgc tttgcttcta
     2161 ttcttaatgt gtttcgttat cggtgcccta caaagatttt cattaattac acactcattt
     2221 attgttgtgt attttctgac atttctctct tgatcaatta ttttaattcc atgggcatcc
     2281 tgatagttca taggagagga gcggctatca gaaatagttg cacctatctc ttgaacttct
     2341 tttacaactt ctggaaaatc catccatcct gcttcagctg tcaaaagtaa taatctataa
     2401 tatttatctt tccttttcaa tctaagaaat cgtcacccca ttctttttct tctattataa
     2461 actatttaac ttttagaatt caaaaaccgt ttgtctattt agagtttacc ctaaatagac
     2521 aaaaatataa cattgaaaaa tttagagttc taacgtatga ttagggtgta ttataaacgt
     2581 tatagaaaag tagggaataa atttgaaaat tgcttatatt agagtttcat ccatcgatca
     2641 aaatgaacaa cgtcaaattg aagaaatgaa aaagtttggc gctgaaaaaa tttttatcga
     2701 aaaacaatct ggagcgacga ttagtcatcg gccgattttc caggaagctt tagattttgt
     2761 cagagagcaa gatattttta ttgtagaagc cattgatcga cttggacgca attatgacga
     2821 gattatcgat tcagtgaatt atctaaagaa gaagaatgtg cgacttatta tcaccagttt
     2881 accgatcatg gctgaagcaa ttggaaatcc tctcctagat aaattcatca aagacttaat
     2941 catttaaatc tcagccatga ttgctgaaca agaacgtact gaatcgaaac gacgccaggc
     3001 acaaggaatt aaaatcgcta aagcaaacgg cgtctataaa ggtcgtccta agttatacag
     3061 tgcagacacc aaagatcctc aacgtcgcct tgtctatagg agtatcgttc aggatcttga
     3121 gaatggggtt gctatctcaa agattgcaac agactacaat gtgactaggc aaacaattta
     3181 ccgaataaaa aaagaaatcg accaactaat tgtatgattt cttttttgaa tcacgtaatt
     3241 attaattagc tttgtcatca atacagacaa tcattttaga ttcatctagc taagttgatt
     3301 acatttatgt taaaaagaat ttagttaaca taaatataat tgatgcataa atcgtcctta
     3361 tttaaaggca tttaacattg acaacttagt ataatgtatc ttatgtaaac taataagatt
     3421 tgaattttat tttatacaca ctcgataata gatcaatttt atggtccccc gcatttacga
     3481 ttgcacaaga gaaacataaa gtcgacctat tagcatttct tttcaattca aacattaaaa
     3541 aagtttatac ctctgctact aaaaaatgaa attatctatt taaaatttaa aacggtaaat
     3601 ctgattcatc caaattggaa aatggatcag cctgtattgg agctttgtat ttctccataa
     3661 taacagatat ctttttttca ttttttaaaa aatacctttt aaatttctta tagtcctctg
     3721 aaatcaaaat atagcgtgat ggatactcat caaaagaaga atcgtattct tcctttaaaa
     3781 ttcccgattt acttaatttt tctagtacat caaatactct atcactcaag taactcctta
     3841 aattgttatc actttcccat tgaactactt ttctataagt tgaattatca gttgaaaaga
     3901 tagaattttc atccataaac gcatatatta gcagcaatct ttcatcattg tcaaattcta
     3961 ttggatcaaa aatatctaag ttgtcatcaa aatctttatc agttccggta acttttttgt
     4021 taattactga aggtgtcttg taagaaatag aatctaaatc aaagcgtttt gattctaaat
     4081 tattttgtgc tttctcaatt aaataattca ctttttttgt gagaaacctt ctgatattgt
     4141 ctttgctata tgttccataa aaacccttat cttctaaaga gtccctgtac ttcttaactt
     4201 ctatatactc ttcagccaaa acttgtggaa ctggttcatc taagaaaaaa acccctacct
     4261 cgcctttgct atttttataa aagtaatcaa tttctgctat agttcccgat ctataattat
     4321 caactggaga acccaactta tggccaaaaa tcgctaataa tatatcacta ttttctacaa
     4381 tttgtttatt aataacactt tgcccagagc tattatttat ataagtagga atcgcattcg
     4441 tttcccatcg aattggcatc aaaattactt ttgtattatc tgtattttct atattccatc
     4501 tatatatggt ttcttctact tcattcctct ttgctgctat atcacctggt gatgctataa
     4561 atacttttat tagatttatg gttttcattt attgtacctt tctctttatg tctctttaaa
     4621 gtaatttttg tataaaattt attgactttt acactatagg acaactttta tctatttagt
     4681 acaaaaaggc ccactaaact aattgattag ttaggagcct attacaggta tattttcaac
     4741 tcgcttttaa gcaaattcta ctaccagctt tttgcctaat gccctagcga tatcatcaag
     4801 tttatcgaaa gatacgttat cccctctctc aattcgagca atagtagatt gagttgtatg
     4861 tgctttttgt gcaagctccg cttgagttag gcctgcttcc tcccgtgcat gataaagagc
     4921 aaccgcagtt tcgagttttc ttccttcacg gtcatatgct tctgcaaaat ctttgtcttt
     4981 ttgcttttct tcaatcaatt ttttaatcat catttgtgaa ccactcctct ctaagttctt
     5041 ttgcatgttt aagttcactt attggtgttt tctccgtttt tttagtaaat ccatgggtaa
     5101 tgacatacct tccattgacc acatggaaat agatagctct ttggatgttt gatcctacct
     5161 tacttcttaa ttcgtacaaa ttagtgtcta attttttcac ccatttcaac cgttgagcta
     5221 ctagcatccc attcttttct gtttcttcta ttgtacgtaa aagcttcgca ctatcttttt
     5281 ttggtaatgt ttccaaccat tctaaaaatt catcatgacc attaggtctt ttaaatgttt
     5341 caaaagatat tttttccata cccaattata tcatatattg attttatatt atagtatata
     5401 acataaaaat tcgaatt
//
LOCUS       DANKHL010000185         5361 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000185 DANKHL010000000
VERSION     DANKHL010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.195"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            20..2347
                     /locus_tag="PW101_002385"
     CDS             20..2347
                     /locus_tag="PW101_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2514761.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2473..2802
                     /locus_tag="PW101_002386"
     CDS             2473..2802
                     /locus_tag="PW101_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDL2514762.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2842..3846
                     /locus_tag="PW101_002387"
     CDS             2842..3846
                     /locus_tag="PW101_002387"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL2514763.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3843..5272
                     /locus_tag="PW101_002388"
                     /pseudo
     CDS             3843..5272
                     /locus_tag="PW101_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
BASE COUNT         1894 a         1030 c         1064 g         1373 t
ORIGIN      
        1 ttttttattg agaggaggga tgaaaatgat taagaaaact caaaataaaa tccaacgtat
       61 atgtcaaaaa cattttgttt ctagactcaa agctgtatct aaaagaaaat acttacatcc
      121 cattatgatt tcgatcatct tgatttttac tttcttaggg agcttatcaa gtatttatgt
      181 aaacgtttat acagaaacgt caaattttgc taaccaaacg acagaggaga caaatagtcc
      241 accctattct gaaatacagt ccatagaaag caccaatcag tctagcaaca acgagacaac
      301 ctcaaccaca tcctcgactc aactgattga tccgacagaa aaagaaatca cagcagcaaa
      361 agcagcaggg gagaaagctt attcagaaac aggtagaagc cagacattta gtactgttgc
      421 ggccactgcg aatttcaacg gacataccat accagatgga tatacagtga tatatgccaa
      481 agatggagtc attgttgcgg ttaacggatc gatgagtgcg gataaaactg cgttacaagg
      541 cgtaaccaaa gcaatcgctt ttattgataa ccgtccagat atccataatc tgtttattgg
      601 ctccacagta aatggtacta ctatcacatt cccaaaaagt atctatatgg gggtggctac
      661 cgctggaaat acgatcgatt cagttacacc caatgcggat aaaagttatt cttttacacc
      721 ctcaacatgg ggagggcttc ctgccggagg aggtatgagt actttttcaa tcggtataca
      781 gttagcaaat gatattccat tcacggggta ctcgatcaaa aatgggcaga tcgttgatgg
      841 taagggaaat gtttcaaatt ttccaaccta tcaagcagca acggttcagg tcaatgcgaa
      901 tcggacagga actttttctt atacagtcaa tggtggagca gcaaaaatcg gaacgtacgg
      961 gactacatta tctggaatcc atgtgggtga caaagtggtt atcactccaa cagcagtaac
     1021 gggctattct tttgcacaaa caaacggttc tttcacagcc ctagaaagta acgtcgatac
     1081 ggtgacctat acaggcaaac aaacaccaat cactattaag tatcaagact attttggaca
     1141 gatgattgct ccttctaaaa taatgaatct tacttacgga agtgctgcac aagacctgac
     1201 gactaatacg cctactatca acggatatac ctttacagct gtttcagcga cagaaacaaa
     1261 gaaccaatca gcaatgagtg ttttagctag tttagataca aatggaaatg taatcgtaaa
     1321 ggacgcaaat ggaaaacaga tttctgaagt catcctatat tataagacaa atgcaacagt
     1381 atcaatcaat gcaaatggca gtaagtacta tgatggtctt tctgttgtac cgatcgttaa
     1441 atatgccttt aatgataaaa cagaatcaac atccctttta aacaatgatc tcagtattga
     1501 tcaaatcaca tggaatccaa ctgatttcaa agcgatgaat gaaaagggag ttgaagtaac
     1561 tgcgcctact gaaattggaa cctatacgtc ttggcaattg acaaaaagcg gactagctaa
     1621 actagctgcc agaacaaact atttatttac tatcgtgcag acatctgatg tctatacgat
     1681 taagcaaatc agcggtgcgg tagagctcag tggcagtaag acatatgatg gaaaagcagg
     1741 tgtcccgtca atccatgtca agctagcaga gggtgtcact agttcgctta ctccagtaca
     1801 agtggcactt agcagtatgg attacacagc agatgcacaa tcagcaaaaa atatggttaa
     1861 tgctggcagc tatacaatta aactaacgaa tagcggaata gacaaagtca aagcagcaaa
     1921 tcccaagttg tcatttactg atatagccaa cacatcagga acttatacca tctataaagc
     1981 taacgcagtg attactgtcg atgacgctgc ctttaattat gatgcccaaa gtcactcgat
     2041 ttcggttgga aatctccacg ttaccggtgt tgtcttaggc gaatcattgg attataccct
     2101 aacagttaac agccggacag atattggatc acaaatagta acgattagtt taggcgaagg
     2161 aagtgtaaac aataattata cgattatcgc aacagatacc gctaagttga cgatcaatcc
     2221 gactctcttg cttccttcga caggtgggat aggtctactg ccctttgtct ttttaggact
     2281 gatctttatt ttttctggct tcttttattt tatccatcgt aagaaaaaag caggtgaaca
     2341 gcgatgaaaa aaacaaaaaa tgtacgtgtg gatatatttg ctgttctctt ttttatccta
     2401 ctggtctttt ttataggttc tccgactgtg tcagctagag aatcacctat tacatttact
     2461 ttgcataatc gaatgttcga aagcgataag atgccaagta tcagtcaaaa cgacggaata
     2521 aatatcccgg ataactttca aggagtaaaa gggttgaacg gcgttacttt cgctatatat
     2581 gatgtctcgg atgaatttta caagctgcgg tcagaaggaa gtagtgtgga agacgcacaa
     2641 agaaaactag cacaaaaaag tgatagtgaa aaaatactgg cagaaaatgt gacaaaaaca
     2701 gtagacgagg aagagggcat tgcttctttt agtgcatcgg acaaagatga gcaaggtcgt
     2761 gacgctgttt accgatttgc ggaaataaag acttcagatt aagtaaaaga aaaatcagca
     2821 ccatttgtcg ttgtgcttcc tgtgacagat tcatctaatc ataaattgac gaccatccac
     2881 ttgtatccaa agagcgaaca aaaaatacct gctgcactca cattaacaaa aacagtagag
     2941 aataagcaga cagattttgc agatggggat aaagttcctt atctcatcac cgtaacaatt
     3001 cctgagaaca tcaacgagat aggaacctat acgatcaaag atacagctga tcctcagtta
     3061 tggctagaac cagaaacgat cgattttttg attggtggag acaagattca tacatttcac
     3121 acacaaaaaa caaaacatgg ttttgtctta tcagattctg gaaaagatct tagttcgttt
     3181 gctgggaaaa aattaacgat ccgttaccaa atgacattga aagaaaacag tgaaaaaacg
     3241 acatttgata acgatgtacg tttgacgact gacaatcaaa cagtagaaac acatcttgcc
     3301 atccaaacag gaggcaaaca atttgtcaaa gtggatcggc aaacgaaaca aaaactagca
     3361 gatgctcaat ttctagtcaa aaaaaatgac caatacttag cgcaagaaaa cgggataaat
     3421 cattgggtag ccaaagatga ttcaagagca acgattttta cttccagtaa agatggaaca
     3481 ttttctgtcc atggattgtc ttttggaagt tacgaactag tggagattaa accgcctaaa
     3541 ggatatatat tgagtcaatc aaccattctc tttgaagttg aaaaaggcag ttatgatccg
     3601 cttcatccta taccattgga aatcatcaac gaaccaaaag caaatccgac gaataatgga
     3661 aaaacgccag ttcagatgca gacaaatggt ccatcaaatg aaaaaaatgg aggaagcttt
     3721 ccaaaaacaa gcgaagagat gcttggaggt ttctcaattt tagggttgtt gctagtcctt
     3781 tcaacaggga cagcttggtt ttataaaaaa caacaaaaag gaaacaatag gagggaaata
     3841 aaatgaaaaa catcaaaaca aaaatatgga cgtttttagg aactgctatc atgctgcttc
     3901 ctttcgttct cggcctagga actgcagagg tttctaggcc gtatcaccta caccagaaaa
     3961 tgttacggtt aatttgcata aattgaaatt cacttcagca ccagaaaatc aaataaacaa
     4021 cggaacggaa ttgacatttc cgaatagcga accgttgaac ggcgttgaat tcaacgtata
     4081 tgacatcact gctacgtact atccaagtaa agacacagca gttccagcag acgctacacc
     4141 cttcgcaagt gtaacgactt ctggtgaagg attagctaac ctgactcttc caggtaaatc
     4201 tgatgggaaa gatgctgtat acgtctttgt agaaacacca aagcccggtg tcgaaacgtc
     4261 gccaaatatc gtgttaagct taccctttta taacccgaat tcatcagacc ctgacaaacc
     4321 tttagacacg attcacctat atgctaaaaa tgtcatcaaa gaaagcaata tcaaaatcca
     4381 gaaagctggg aacttttcag gggtagataa gttagcaggg gcgcaattcc agatcaaaaa
     4441 ttcagagaat caattcgtga ccggttttga tgaaaacact ggtgtcgctc tctatgatcc
     4501 tgctgagtca gcgttgacat tcacaactga tactgaagga tccatcagtg tcaatggatt
     4561 aatagacggc aattatactt tagttgaaac aaaagcacca gatggttatc aattaaccaa
     4621 tccacaaaaa gaaactactt ttatagtaaa aaacggaaaa ttgcaacaaa caaccggtat
     4681 catccatggc gcagatgaag ccaatcttgg gaatccaaac ggtgtagtca atcgggaaaa
     4741 tacaactacg gataacacga ttactgtaga aaatgtccaa aactacggtg atttccactt
     4801 catcaaacaa gatgtgaaca cgcaagaaaa attagccgat gcagaatttt ctattctaag
     4861 agcaaaagat ccaactgtta aattaatgaa aaaaatagca aatcttgcct caggagaata
     4921 cccatatgct tggtcagatg aagtcacagg ttctgactgg gaagaagtga aattgatttc
     4981 tgattcagaa gggaaatttt caatcgaagg gctgaaacaa ggggcatact gccttcaaga
     5041 aacaaaagca ccagaaggct acgcattacc taagaatgaa gctgcgtaca ctgaatttac
     5101 agtagatgat gatccaacaa cttctgatga aacaacgatc gataaccaac ctaaaggaat
     5161 cttgccttca actggtggta tggggatcat cgtattcatc ttggtaggta ctgctttagt
     5221 tggaggagca gtgatctact tcaaaaaacg ccatcaagaa acagaagctt aaactattgg
     5281 aaacaaaaat catacgataa ataaacgata gtgattcaaa tggggagaaa ggttgtgaca
     5341 agtggtcggt ctcttggtat t
//
LOCUS       DANKHL010000186         5312 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000186 DANKHL010000000
VERSION     DANKHL010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.196"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="PW101_002389"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="PW101_002389"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDL2514764.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="PW101_002390"
     CDS             complement(1297..2133)
                     /locus_tag="PW101_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2514765.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="PW101_002391"
     CDS             complement(2317..3534)
                     /locus_tag="PW101_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2514766.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3862)
                     /locus_tag="PW101_002392"
     CDS             complement(3683..3862)
                     /locus_tag="PW101_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514767.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3917..4000
                     /locus_tag="PW101_002393"
     tRNA            3917..4000
                     /locus_tag="PW101_002393"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..4818)
                     /locus_tag="PW101_002394"
     CDS             complement(4240..4818)
                     /locus_tag="PW101_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514768.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(4824..5207)
                     /locus_tag="PW101_002395"
                     /pseudo
     CDS             complement(4824..5207)
                     /locus_tag="PW101_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1654 a         1082 c          838 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca ta
//
LOCUS       DANKHL010000187         5143 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000187 DANKHL010000000
VERSION     DANKHL010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.197"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..385
                     /locus_tag="PW101_002396"
     CDS             <1..385
                     /locus_tag="PW101_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2514769.1"
                     /translation="RFVTDIPAGQIVLSDPRLLQKALSNVILNAVQNTPQGGEVRIWS
                     EPGAEKCRLFVLNMGVHIDDTALPRLFTPFYRIDQAQSRKSGRSGLGLAIVQKTLDAM
                     SLQYALENTSDGVLFWLDLPLTSTL"
     gene            556..1362
                     /gene="vanY"
                     /locus_tag="PW101_002397"
     CDS             556..1362
                     /gene="vanY"
                     /locus_tag="PW101_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HDL2514770.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            1380..2207
                     /gene="vanW"
                     /locus_tag="PW101_002398"
     CDS             1380..2207
                     /gene="vanW"
                     /locus_tag="PW101_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HDL2514771.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGEPFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            2204..3175
                     /locus_tag="PW101_002399"
     CDS             2204..3175
                     /locus_tag="PW101_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HDL2514772.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            3168..4196
                     /gene="vanB"
                     /locus_tag="PW101_002400"
     CDS             3168..4196
                     /gene="vanB"
                     /locus_tag="PW101_002400"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HDL2514773.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGTFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            4202..4810
                     /locus_tag="PW101_002401"
     CDS             4202..4810
                     /locus_tag="PW101_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HDL2514774.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(4876..5049)
                     /locus_tag="PW101_002402"
     CDS             complement(4876..5049)
                     /locus_tag="PW101_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514775.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1436 a         1100 c         1364 g         1243 t
ORIGIN      
        1 gcggttcgtc acagatattc cagccgggca aattgtcctg tccgatccga ggctgctcca
       61 aaaggcacta tccaatgtca tattgaatgc agttcagaac acgccgcagg gaggcgaggt
      121 acggatatgg agtgagcctg gtgctgaaaa atgccgcctt tttgttttga acatgggcgt
      181 tcacattgat gatactgcgc ttccaaggct gttcacccca ttctatcgca ttgatcaggc
      241 gcaaagcaga aaaagtgggc gaagcggttt aggacttgcc atcgtacaaa aaacgctgga
      301 tgccatgagc ctccagtatg cgctggaaaa cacctcggat ggcgttttgt tctggctgga
      361 tttaccgctc acatcaacat tgtaaatatt taaaacttaa atgattttga ccgacaggta
      421 taaccctgcc ggtctttttt tgctgctaca ggaaaactac agactggcta cagggaaagt
      481 acagatgcgc ttgccataat aacaatcgta ccagccacaa atcgtagttt tattgcaaag
      541 gaggcattcg atcaaatgga aaaaagcaac tatcattcca atgcggatca tcacaaacgg
      601 catatgaaac aatccgtgga aaagcgggct tttctatgtg cgttcattat ttcgttcaca
      661 gtctgcacgc tgtttttggg gcggagactg gcttccgtat tggaggcaac acagataccg
      721 cccatccctg caactcatac aggcagcagc actgacgtag tggagaattt ggaggaaaac
      781 gctcttgcca ccgccaaaga acagggagat gaacaggaat ggagcctgat tttagtgaac
      841 aggcagaacc ccatccccgc acagtacgat gtggagcttg agcaactatc aaatggtgag
      901 cggatagata ttcggatttc tccctatctt caagatttgt ttgatgccgc aagaactgat
      961 ggagtttacc cgattgtcgc atccggatac cgaacaacag aaaaacagca agaaattatg
     1021 gatgaaaaaa ttgccgaata taaggcgaaa ggctacacct ctgcacaggc taaagcggaa
     1081 gcagaaactt gggtggccgt gccgggaacg agcgagcatc agcttggtct tgctgtggat
     1141 atcaatgcgg acggaattca ttcaacaggc aacgaggttt atagatggct ggatgaaaac
     1201 agctatcgtt ttggttttat tcgtcgctac ccaccagaca agacagagat aaccggtgtg
     1261 agcaacgagc cgtggcatta ccgatatgtt ggcatcgaag ctgccacaga gatgtacaac
     1321 caaggggttt gccttgagga atatttaaaa ccagaaaaat gagaaaagga tgttatgtta
     1381 tggacagaaa aagattgaca cagcgtttcc cgttcctgct tccaatgaga cgggcgcaga
     1441 gaaaaatgtg cttttatgcg ggaatgagat ttgacggctg tcgctatgcg cagacgatag
     1501 gggaaaaatc gctttcccat ttgctctttg aaacggattg cgcattatac aaccacaata
     1561 ccggatttga catgatatac caggaaaaca aggtgttcaa cttaaagctg gcagcaaaga
     1621 ccttaaacgg cttattgata agaccggggg aacccttttc tttctggtgg ctggtacgcc
     1681 atgcggacaa agataccccc tataaagacg gccttacggt gaccaatggt aaactcacca
     1741 ccatgtcggg cggcggtatg tgccagatga gcaatttact attttggatg ttcctgcata
     1801 cgccattgac aattatccag cgcagaggtc acgaagtaaa ggagtttcca gagccaaaca
     1861 gtgacgagat taaaggggtg gatgcaacca tttcagaggg ctggattgat ttaaaagtgc
     1921 gaaacgatac cgactgcacc taccaaatat gggtgacctt agatgatgag aaaatcatcg
     1981 gtcaggtgtc cgccgacaaa gagccccaag cattatacaa aattacaaat ggcagtatcc
     2041 agtatgtccg tgaaagtggc gggatttatg aatatgccca ggttaaacgg atgcaagttg
     2101 ccttaggtac cggggaaata atagattgca agctgcttta tacaaacaaa tgcaaaatct
     2161 gttatcccct cccggaaagt gtggatattc aggaggagaa ccaatgagaa aaagtgtggg
     2221 cattactgtt tttggatgcg aacaggatga ggcaaatgct ttccgcgctt tatcgccgga
     2281 ttttcatatt atccctacgc tgattagcga tgcgatatcg gcagacaacg caaaattggc
     2341 cgctggcaat caatgcgtta gcgtaggcca taagtccgag gtttccgagg cgacaattct
     2401 tgcgctgaga aaggtcgggg taaaatacat ttctacccgc agcatcggct gcgatcacat
     2461 tgatacgact gccgccgaga gaatgggaat ctcggttggc acggttgcgt attcgccgga
     2521 cagcgttgcg gattatgctt tgatgctgat gctgatggcc atacggggtg caaaacccac
     2581 catgcacgcc gtggcgcaac aagatttcag attggatcgt atccggggga aagaactggg
     2641 ggatatgact gtgggagtta ttggaaccgg ccatatcggg caagcggtcg tcaaaaggct
     2701 gcggggattt ggatgccatg tgctggccta tgataacagc cgaaaaatgg atgcagatta
     2761 tgtccagctt gatgagcttc taaaaaacag cgatattgtt acgctccatg tgccgctttg
     2821 tgcggatacc cgccatctga tcggtcagaa gcaaattgga gagatgaagc aaggcgcatt
     2881 tttaatcaac accgggcgcg gagcgcttgt cgataccggg gcgctggtgg aggcgttgga
     2941 aagcggaaag ctgggtggtg cggcactgga tgtgttggag ggcgaggatc agtttgttta
     3001 taccgattgc tcgcagaaag tgcttgacca tccctttttg tcgcagcttt taaggatgcc
     3061 gaatgtgatc atcacacccc atacggcgta ctacactgag cgtgtgctgc aggatactac
     3121 agaaaaaaca atcaggaatt gtctcaattt tgaaaggagt ttacagcatg aatagaataa
     3181 aagtcgcaat catcttcggc ggttgctcgg aggaacatga tgtgtcggta aaatccgcaa
     3241 tagaaattgc tgcgaacatt gatacggaaa aattcgatcc gcactacatc ggaattacaa
     3301 aaaacggtgt atggaagcta tgcaagaagc catgtacgga atgggaagcc gacagtctcc
     3361 ccgccatact ctccccggat aggaaaacgc atgggctgct tgtcatgaaa gaaagcgaat
     3421 acgaaacacg gcgtattgat gtggctttcc cggttttgca tggcaaatgc ggggaggatg
     3481 gtgcgataca ggggctgttt gtattgtctg gtatccccta tgtgggctgt gatattcaaa
     3541 gctccgcagc ttgcatggac aaatcactgg cctacattct tacaaaaaat gcgggcatcg
     3601 ccgttcccga atttcaaatg attgataaag gtgacaagcc ggaggcgggt gcgcttacct
     3661 accctgtctt tgtgaagccg gcacggtcag gttcgtcctt tggcgtaacc aaagtaaacg
     3721 gtacggaaga acttaacgct gcgatagaag cggcaggaca atatgatgga aaaatcttaa
     3781 ttgagcaagc gatttcgggc tgtgaggtcg ggtgtgcggt catggggaac gaggatgatt
     3841 tgattgtcgg cgaagtggat caaatccggc tgagccacgg taccttccgc atccatcagg
     3901 aaaacgagcc ggaaaaaggc tcagaaaatg cgatgattac agttcccgca gacattccgg
     3961 tcgaggaacg aaatcgggtg caggaaacgg caaagaaagt atatcgggtg cttggatgca
     4021 gagggcttgc ccgtgttgat ctttttttgc aggaggatgg cggcatcgtt ctaaatgagg
     4081 tcaataccct gcccggtttt acatcgtaca gccgctaccc acgtatgatg gccgccgcag
     4141 gaatcacgct tcctgcactg attgacagcc tgattacatt ggcgttaaag aggtgataag
     4201 tatggaaaat ggttttttgt ttttagatga aatgttgcat ggcgtccgtt gggatgccaa
     4261 gtacgccaca tgggataact tcacaggaaa accggtggat gggtatgagg tgaatcgcat
     4321 catcggcaca aaggccgtgg cgtttgctct gcgcgaggca caaatccatg cggctgcgct
     4381 tggctatggc ttgcttttat gggatggata tcggccaaga actgcggtgg actgcttcct
     4441 gcgttgggca gcgcaaccgg aggacaatct cacaaaagaa aaattttacc ccaatataga
     4501 gcgagccgag ttgattacaa agggttatgt ggcctcacaa tccagccata gccgtggaag
     4561 cgcaattgat cttacgctct accacctgga tacaggggaa cttgtttcaa tgggaagtaa
     4621 cttcgatttt atggacgaac ggtcgcatca tacagcaaaa gggatagggg atgcagaggc
     4681 acaaaatcga agatgcttgc gtaaaatcat ggaaagcagc ggatttcagt cttatcgctt
     4741 tgaatggtgg cactataagt tgattgatga gccatacccc gatacctatt ttaattttgc
     4801 cgtttcataa tgaaagtatt ggttttgtat cagattttgg atttgataaa atctatgctg
     4861 gtcatggaca atcttttact ggaaagtggt taaagcatct tttgtaatac attgcaaatt
     4921 atagtattca atctgcccag cggcagaaaa gaaaaaaacc gctgttgggc agacctttcc
     4981 cacgcccatt ttgtattttg ctggcggcgg ttttgagaaa tcaaggccgc cgttcttttc
     5041 tggcagcaaa aggtgtggcg gcccatagga ggatgccgct atgctgtttt gctattgcca
     5101 tgctctaatc cacttccacc actcaaaaaa agccttgccc ctc
//
LOCUS       DANKHL010000188         5100 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000188 DANKHL010000000
VERSION     DANKHL010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.198"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(405..779)
                     /locus_tag="PW101_002403"
     CDS             complement(405..779)
                     /locus_tag="PW101_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2514776.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(802..2202)
                     /locus_tag="PW101_002404"
     CDS             complement(802..2202)
                     /locus_tag="PW101_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HDL2514777.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2206..2616)
                     /locus_tag="PW101_002405"
     CDS             complement(2206..2616)
                     /locus_tag="PW101_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2514778.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2634..3125)
                     /locus_tag="PW101_002406"
     CDS             complement(2634..3125)
                     /locus_tag="PW101_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2514779.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3135..3926)
                     /locus_tag="PW101_002407"
     CDS             complement(3135..3926)
                     /locus_tag="PW101_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2514780.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3919..4692)
                     /locus_tag="PW101_002408"
     CDS             complement(3919..4692)
                     /locus_tag="PW101_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2514781.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4848..>5100)
                     /locus_tag="PW101_002409"
     CDS             complement(4848..>5100)
                     /locus_tag="PW101_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2514782.1"
                     /translation="KYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHLKEGAQLF
                     DTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
BASE COUNT         1627 a         1031 c          672 g         1770 t
ORIGIN      
        1 taggagtgag catttttcga aaaatactaa aaatttttca aaataaaaaa aggcatacag
       61 acaataaact gtctatacgc ctcacagact ctattctata catctaattt tggctgtcct
      121 gcaaaaaata tgtgttagcc tccatcgaaa gatcaaactt tggaaaacat cattataact
      181 ttgcttggta agagctttgc taaaacatga tatgacttgt atacaaaatg tggagtgtat
      241 tccaataaat aggaaggatt acaaaatttg aaaccaaata agtaaaaaat actgtcactt
      301 tgaattaatt tgaaatagta aattctcatt aaactattga aatgctgata ccaaaaaatg
      361 aagtactttt cgttcatacc aacttagagc acttcgttat ctatttattt cttcatttgg
      421 cttatctcta taatctcatg atttggtccg tagaaattaa agtaacggat tccattttcc
      481 cagaacggta attccataat atttttatct aataattcat atcctaaatc ttgaattgaa
      541 ttgaatacct catctatgtc atccgtatct aatgctagat gatcaatggc tcctctacac
      601 tcagtagttt tatcttccca tatttctatt atcgttccat tcaactcgaa aaagccgact
      661 ttacttccca cattcatttc ttcattaatc agactaaacc ctaaggattc ataaaagatt
      721 tttgaagctc caaaatcttt tgttggaatt ccaatatgtt gtaaaccatt taatttcact
      781 tacatcatcc cttcaattgt tttaaaatcc acattcataa tttattgaat agtctagttt
      841 atctaccttg attgctccct ccgtaacagt catacttaat gctgttccga ctttatcata
      901 cattctagta cttaaagcaa cattatcaat atataaaaca gctatcgtat catcaacaat
      961 taactttagt tcatattctt tgcctatttc caaaaatagt ggtcgttgca gccccttatc
     1021 aaacatttga ggccaaggat agttcggagt ttttccgaaa gtaagctgat tattaatcaa
     1081 tgaaatacga aattcgtatc cttcttgagt atcactatct acaaataatt ttaaagcaaa
     1141 ctcctttgtt aacgatgtaa tagtcaaagt tgtttcaaat agaaaataat cttcgatttt
     1201 aggaacaatt actctttctt tttttccaaa ttctttctct aacttaaaac ttgaaaatgg
     1261 agtgatttct ttaaaattct caattatttt ttcaggcagt ttaacaccaa gagttttgtc
     1321 tgtcttttga ataatttgat gtggaacaag tgcaccgccc caatcccagt tattttgatc
     1381 gttctcccct tcttttgttg ccacccagcc agttagaaaa cggccagttt cattagttcc
     1441 gactgttctt gctgcataat aggcttttcc gtcgaaagct tcgtctactg gtgatttcca
     1501 atctgaaaat aatcctgctc cgactcgata tttagtcttt ttatcttcac aatactctga
     1561 aaaaagtaaa taccacgttc catcaatttc aaatatatca ggcatttcaa tcatgttaaa
     1621 ttcattatta gcccagtagt ctcctttgaa attccaatct tttaaatctt cggattcaaa
     1681 atagaccaat ctccccgtcg gctttttctt acttgctggt tttctagctc ctagtatcaa
     1741 aacgtatttt ccatgatctt gatcaaaaat tgcaaaagga tcgcgccaat cattcttatc
     1801 atatccttct tgaggaacaa gttttgctgc ttcgccatcc tttttccaat gaattaaatc
     1861 tttagaactt gcaatcatta acacttctga agtttttcca tccaacttag ctttgcgatt
     1921 atgtccagta tacaaagcta aatactcatt gattccttgg acaatacttc cagcgtaaat
     1981 gtattgatca atgtcatcgt tgccaccacg atgaattact tccccgtatt cttcaaattt
     2041 cacaaaatca gtggttgtaa ctagtgacca cccaaaagga tcagttaaag gtttaggatt
     2101 tctcgtatct cgttgatgaa atagataaaa agttccatct ttataaaaag gcatgcaatc
     2161 cccaaaccaa aaatctttag gcttgtaaaa aattttttgc atttatcact catcctcttc
     2221 gttagtatgt ctttgcatat ctgcaagaca cgcaattgta ttcatatttc ttagattttc
     2281 gatgatatca tcgctttttt ttccttgtaa tctcaaattg acagcctcaa tcaaaattcc
     2341 taaattcatg ccaccaagaa gttgaaaccc attttgaaat acttcacttc ttttagcaaa
     2401 ttcgttgaaa ggtgttccac caatcaagtc agcaaatacc ataacttctt gattttttac
     2461 ttgagaaact attttttcaa tgttttcacg gaattcttca atgcctgcat ctccaagtgt
     2521 taccacatga caatttaaat tatcgccaaa aaacattttt aatgtatcta caaatccaat
     2581 ggcaatattt ccatgagatg taattataaa ctccatccac tcatcctctg ttcttaataa
     2641 ataaaatcct ttaaaaattc ttttgaatct ctaggagttt gtatcactgt cacttcaact
     2701 ccatcactaa ttagttcttt aaaagcctct ctctcttctt cagtaatatt tatcgtcgga
     2761 cggatgcgtt ctgttccatt ccttcttgat aaatttccta cgtttacact tgttattggt
     2821 aaaccgttat caatcaaaaa tttaacatcg attggagaaa ctgcaacaat aagaactttt
     2881 tgtccttcat atttaccaga aagaatattt ttaactgcta cttcccttgt aattattgaa
     2941 gttctaacat taggtggtgc agcgaggcga actacagctt tttttacgtc atcattggct
     3001 acttcattat taataaccat aattctgtta gcactcaatt gatttgtcca ttgtgttgct
     3061 acttgtccat gaatcattcg atcatcaata cgaacatgta taataccttt cattttttca
     3121 tccatattca acacctactt caaaatattt aacgctctta acgcaataga aactactaag
     3181 attaatagaa ttacataaac tggtttaacc tttcgtccta acaaataata cacaattgct
     3241 actacacatg caggtaataa accaggcata acttgatcaa agatttcatt tccggtcatt
     3301 gttaagtcgc cttgtttaaa ttcaaagcca acattcactt ttacaactgt ggcaatgagt
     3361 gcaccgacca ccattaaccc taaaattgat actgcatcag taatagactt taattttgtt
     3421 tcaatttgag ttagtaggct cgcacccgat tctttcccag caaggtataa aggataagca
     3481 gtaatcctta ataaaattga tgccagtatc cacaaaataa ttccaacaaa actaccttcc
     3541 aatgccatat tagctgcaat tgctccaaaa atagtccatg gaataactac aaatacacta
     3601 tctccaattc ccgctaaagg acccattaga cctgttttca gagcacttac tgcttccaaa
     3661 gacttatttt tttcttcttt ttcaattcca acattcatac caataatgta aggcgctgta
     3721 tacggcgtag tgttaaaaaa ttgtaagtga gctaaaacgg attgtttcat tccttcagga
     3781 tcatctttat atgtgtcctt gataaaagga agcatagaat acgcataacc caacccttcc
     3841 attttttcat aatttaatga agttgcacca ttgagaatcc accggaaaat tacttgtttg
     3901 tcgatttttg tcagtttctt attcatcttc gtcaaaacct cctgttgcag ctggtgctac
     3961 ttgagtatta tttgatctat ttttatatac gattagtgcg attgcagcgc caagtaacga
     4021 aatccctaaa attggtattt ggaaataaac tgccaaaacg aaaccaacta acaaataggc
     4081 aaagtaatct tttgtaggta aatatttaag tagcattcct atcccaacag caggtaatag
     4141 tccccctgct acctttaaac caccggtaac ccattcagga atataattga ttgcacttcc
     4201 cactaacgtt gggcctaata tcacagttaa caaaacagga attccagata caaagcttgt
     4261 attaattgat gccaaatatt gcatccactc taatttttga taatcttctt tttgtgcgaa
     4321 tttttctgcc ttttgttgaa aaaaaatgtt tgttgaccac tttagaacat ctagttgtac
     4381 aattaacatt gctaccggta ttgctaaagt tattcctata gaagcatcct ggttagttga
     4441 gattgctaaa taagtaccga tcaatgctgc tgtttgataa tccggtactg atgcaccacc
     4501 aaaactagta atacctagtg tcattaattg caaagtccca ccgatataaa gtccagtttg
     4561 aatatctccc ataataagtc cggtcaagaa acctgcaacg acaggttggg aggttccgat
     4621 tttgggccct tccttatccc agttaatgaa aaatccatac aacacaacta aaaatgtttg
     4681 ccaaaacgcc attttgttct ctcctttctt cttatacttt tatataagca attactgtgc
     4741 caactaacat tatccaataa agcgggaaaa tacagtaaat attaattaaa tgagcgtcta
     4801 aatatacttt ggacaccctt tcattttttt agacgctagt atgctattta gaaaatagtc
     4861 tgctcaaaaa ttgtatagat taactcatac tctcctttgg gtatttcaat acagtaaata
     4921 tctttgattt ctgaaaaaaa atcttttgat acatttaaaa aagtatcaaa tagttgcgct
     4981 ccctctttca aatgttcatt tgttattcca tcaccaatca taattcgttc aatcattcca
     5041 gaaagatgta gaaatagatt aattctcaga aaacttggga ttttgacatc gtaatatttt
//
LOCUS       DANKHL010000189         5095 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000189 DANKHL010000000
VERSION     DANKHL010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.199"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            178..1893
                     /locus_tag="PW101_002410"
     CDS             178..1893
                     /locus_tag="PW101_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2514783.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            1896..3659
                     /locus_tag="PW101_002411"
     CDS             1896..3659
                     /locus_tag="PW101_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2514784.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(3699..4235)
                     /locus_tag="PW101_002412"
     CDS             complement(3699..4235)
                     /locus_tag="PW101_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL2514785.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(4558..4980)
                     /gene="mgsA"
                     /locus_tag="PW101_002413"
     CDS             complement(4558..4980)
                     /gene="mgsA"
                     /locus_tag="PW101_002413"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDL2514786.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
BASE COUNT         1528 a         1022 c          980 g         1565 t
ORIGIN      
        1 tgttttctca ttttcttttt tttaaaaaaa atagtctatt caatttctaa agcttcttca
       61 taataaaact ttcatttatg aaaaggcttg ttctcttttc attctaaatt tcctaagcta
      121 caatgtggta gtacttgttt tttcaattgt ggacaaataa ataataggag gaatcatatg
      181 attcagttat tacgttatgc aaaagactat cgtaagcaaa tcattttggg cccgttcttc
      241 aaatttcttg aagctgtctt cgaacttgtc ctgcctttaa tgatggcttc cttgatcgat
      301 aatggactaa aaatgaatga ccgaggaaaa atcattgaaa tgggcttatg gatggtcgct
      361 atgtctgtga ttggtctgat ttgtgctatc atttgccaat actatgcttc agtagcttca
      421 caaggatttg gtacagagtt acgtaatcag ctaatcaaaa aaatcaatac tttctctcac
      481 aacgagttga atcattttgg aacagatacc ttgatcaccc gaatgacaaa cgacatcaat
      541 cagcttcagc tagctttagc aatggtcatt cgtttattga tcagagcacc gtttttaagt
      601 atcgggtcag tggtcatggc ttttgtgatc gactgggaag taggtctgtt tttcttagca
      661 ttattgccta ttttctcaat cattttattt tttatcatca aaaaaaccgt tcctctctac
      721 caaaaagtgc aagaaaaact agatcagctc aatgaacacg ttagtcagaa tcttagcggt
      781 gttcgtgtca tccgcgcatt tgcgcagaca aaaaaagaaa cagagaagtt cgataaaagc
      841 acaaatgatc tagcgaacaa ttatttacgt gtttccaata tctctgccct attatcacct
      901 gctacaacct tgatcatgaa tgtcgggatc atctgtttgc tgacagttgg cgggatcaaa
      961 gtaaatattg gtagcttgca acaaggacaa gttcttgctt tgatcaatta tatgaatcaa
     1021 atgcttttgg cactgatcgt tgtttcaaac ctcgtcgtga tcttcacacg tgccgaagct
     1081 tcaggaaatc gggtgaaaga ggtgctagac acagaaaatt cgattcttga agctgaggtc
     1141 agtactactc ccgatttttc ttctgaagca attatcgctt ttgaccatgt tgacttccgt
     1201 tatactcctg attctggtct ttctctccaa aatattacat ttcagctgaa gcgaaataca
     1261 gtccttggaa tcattggtcc aaccggaagc gggaaaacga ctttgacaca attgattccg
     1321 cgtttctatg atgtcagttc cggtacagtt ataatggacg gaatggatgt tcgttcttgg
     1381 cagctggatc atctccgtca gcaaattgct cttgttcccc aaacttctgt actatttacc
     1441 gggactattc gtgaaaacct tcaatggggt aagaaagacg ccacagaaaa agaatgttgg
     1501 gaagctctga aaatcgctca ggcagaagaa tttgtccgtc agcttccaaa tggtttagat
     1561 acaaaagtca tggaaaatgg gaaaaatttc tctggcggac aaaaacagcg actgacgatt
     1621 gcaagagctt tgattgccaa gccagcatta ctgattttag atgactcttt aagtgcgttg
     1681 gattatcaaa cagacttgga tttgcgtaag gcgctacaga cttatcttgg cagtacagtg
     1741 attattatct cccaacgtgt ccgctctatt caagaagcac atcatatctt agtgatggat
     1801 caaggtaaga ttgctgctca gggtacccat gaagaattac ttgctcaatc agcagaatat
     1861 caggaaatcg tcgcatcaca ggaggaagaa tagttatgaa agtaaaaaaa ttatccttgc
     1921 agacgctccg ccgattcatt ccttacctta ctgcctatgc aaaagacatt tggctagctg
     1981 tgatactagg aatcatcaat gggattgcaa cagttgtgat gacgttacaa attggtcaaa
     2041 gtatcgatca aatgattgga gaaggacagg tccgcttcaa tcgactgttc catttactta
     2101 ttctattcgg tggtatcgtt ttaatcaacg tagtcagtca atggctcatc caagtgttaa
     2161 gcaatcggtt agcttactta tccgttgcaa aattaagaaa agatgcattt gctcatctga
     2221 atcgattgcc acttcgttat tatgatcaaa actcccacgg aaatatcgtc agtcgattta
     2281 caaatgatat ggacaatatt tccattgcaa tttctgcggc cttcaatcaa ttattttctg
     2341 gagtggcaat catcattctt tcgtttattt ttatgatgcg tctaagtcca cttctgacat
     2401 tggtcgttct ttgttctaca ccaattattt tccttgtgag ttggatcgtt gctcgtgctt
     2461 cacaaacaaa tttcgataga cagcaaagga ttattgggga tatctcgggc tttgtgaccg
     2521 aacgaatcgg taaccagaaa atcgtgaaag cttttcagca agaagaagct agtcaacagc
     2581 aatttgaact gctaaatgct gatctatatg aaaaaggaca acgggcacaa ttttcttctt
     2641 ccttgacaaa cccgtcttct cgttttgtag atcaccttgc ttatttatcg attggtttag
     2701 ttggcggcct attggcattg aagagtggtt cgtcaatcac tgttggtgtg atttccagct
     2761 ttacgatcta ctcaagtcag ttttctaaac catttatcga actttctgga atcacgacac
     2821 aactgcaaac cgctttttct ggtctagaac gagcatttga tttgatcgat cagccagaag
     2881 aaaagcctga tttgccagat gcctatgcct tggaacccaa aaatatcaaa ggaaaagtct
     2941 cttttgatca tgtttctttt tcttatgacc ccaagcagcg tttgatcgaa gatttcaatt
     3001 ttactgctta tccaggtcaa accattgcta tagtaggaaa aacaggggcc ggaaaatcca
     3061 cacttgttaa tttgctgatg cgtttttatg acacaacaaa aggaacaatt acagtcgatg
     3121 accatgacat ccgctctatc caacgtgata cattacgtaa aagttttgga atggtgttac
     3181 aagatacttg gctatttgac agtacattgc gagaaaatct tcgttttggg aatccggaag
     3241 caagtgacgc agaaatcaat gatgccctaa aagcctctta tatgtatgaa tttgtagaac
     3301 gtttgccgga aaaattagat actaagattg gcggtcaagg gttaaagata tcagatggac
     3361 aacgccaact gttgacgatt gctcgaacga tgatcagtaa tccccctatg ttgatcttag
     3421 atgaagctac cagctcagtc gatacattga cagagaaaaa aatccaacaa gccttcttag
     3481 caatgatgga aggaaaaacc agttttgtga ttgcccatcg cctgtccaca atcaaaaatg
     3541 cggaccagat attagtgatg gatcagggag ctattgtcga aatgggcacc catgatgagc
     3601 tgcttcaaaa agatggttat taccgacggc tctatcaagc acaattcaca gcacaataaa
     3661 aacaagcagt cttctgggat gggaagactg cttgtttttt atttagcgtg gatcgctaaa
     3721 cttcttaaaa tttttttgta aggaatacta cgtcctacgt aataaatggc aattgattga
     3781 atcggtaacc atattactgt ttttaacagg cggggcgccc aaacgaccca gctgaacaat
     3841 ggcacatggt acatcaacgc aagccataat ggtgtcaaaa ataagttgat gatcagcgtg
     3901 acactaagcg tagccagaat ggcatttttc caagtgatct cttttcgata gaaaaagaat
     3961 ccgtagattg ctccttcaat aaatgcattc aatgtaaatc caataaagaa tgcagatttc
     4021 gagaagaggg ccattcctac taaatcagct aaaactgcgc ctatccctga ccaaaaagga
     4081 ccaaaaagaa tgcccatgat cacttgtgga ataaatgtga cactgatttt aataaatggg
     4141 gtctcgattg ctaccagtcg ggacaaagtg accatcaatg caatcaataa gcccataata
     4201 gcaatcatac gtgcgtctaa ccggtgtttc ttcattttta gcatctcctt taagagctgc
     4261 tacatcgtat catgcagcga atgcgaaaat aaaaccgcga acattacgtc cttcgtccaa
     4321 gaggcaacat cccatcctct tagcacttaa cgctttattt tctactctgt attatttttg
     4381 ggatcttttc ccacagaaac tatagcatat tttcacattt actgcaaata cgaaaaacta
     4441 atattattgt ttatattatc caatacgttc gtattatatc gcatattatc agtttgttta
     4501 gcttttatag ctacatatag gcgaacatta aacgttattt ttccatacgc ttatccatca
     4561 aaatgaaagt ggatgcttgt ctatttcatg gacgattctt cgaaaatcta ggaagccggc
     4621 ttccagtcct cttagtagca cctcagctgt tccgatattc gtagctaacg gaatctcata
     4681 gacatcagat aaacggataa gtgctgttac atctggttca tggggctgtg cagccagcgg
     4741 gtctcgaaga aaaatcacca aatctaactg atcctccgaa atcatggctc caatttgctg
     4801 atccccgcct aacggtccgg atttaaaccg atggatcggc aagccggttg cttccgtgat
     4861 cctctgccca gtcgtcccgg tagcaaaaag ttcatgtttt tccaatattg ttttatacgc
     4921 tgttgctaat ttcaccatca taggtttttt tcggtcatga gcgatcaaag cgattttcat
     4981 aagagacact ccttttgata ttgttaacat acttatttac tccagtgtat cagtttttat
     5041 ttcttatgga tatttattaa agcttaaaac tagcacattg acaaaaaaaa gaaaa
//
LOCUS       DANKHL010000190         5088 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000190 DANKHL010000000
VERSION     DANKHL010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.200"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(205..660)
                     /locus_tag="PW101_002414"
     CDS             complement(205..660)
                     /locus_tag="PW101_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514787.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDD
                     EYYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIAR
                     NTSASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
     gene            complement(1269..1685)
                     /locus_tag="PW101_002415"
     CDS             complement(1269..1685)
                     /locus_tag="PW101_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514788.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTITTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            complement(1687..1968)
                     /locus_tag="PW101_002416"
     CDS             complement(1687..1968)
                     /locus_tag="PW101_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514789.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            complement(2415..3383)
                     /locus_tag="PW101_002417"
     CDS             complement(2415..3383)
                     /locus_tag="PW101_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2514790.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            complement(4550..4705)
                     /locus_tag="PW101_002418"
     CDS             complement(4550..4705)
                     /locus_tag="PW101_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002402449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514791.1"
                     /translation="MKLTKLTISLVIIAGIILYFVLPANQFFLYFGIPTILIVNGVRI
                     FEQRKKM"
     gene            complement(4724..>5088)
                     /locus_tag="PW101_002419"
     CDS             complement(4724..>5088)
                     /locus_tag="PW101_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2514792.1"
                     /translation="FSVLFLINILNTSFSEGINPNEVNGSLLLFFLNAATYGAFLEEG
                     IFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFVYIQTKNIWYSI
                     VAHGFYNTIGILIYLISI"
BASE COUNT         1595 a          839 c          839 g         1815 t
ORIGIN      
        1 aaaaaaaact atatttaaag agttttttta gatttacttc tttgaattca cgaatgaagg
       61 tagaatgaaa tagtataatt attagcacta aaaaaaatat atcactatgt tcaaataaaa
      121 aatatatatt tgacggtact cctaatgatg ggatagcaaa ggtcaaaacc aagtaagcaa
      181 tactaattat ccattttatt gtcattaggc aggaatataa tcaatatatt gtgtatttgc
      241 tgtagaagtt tgagtacgaa tatatttatt atctacctta cgataaactc tgtaggttac
      301 agttattctt gcattttgac gatatttttt aaattttcca cttgcagaag tgtttctggc
      361 tatttcgatt ttcatcccaa atacatttgc actgacagat aactttgtac ctcgactatg
      421 agtttctata cctaaataag tacggcttcc tttagcaatt gaccagcttt gaggtttaga
      481 ccaagtcctt gtttgaataa ctggttcgtc taacacatag tagtactcat catcagaata
      541 agtggctata tcagaaatgt tgttctcatt agcgaatacc gctccgctag gagtaatagc
      601 agaaccaaaa gttaaaatag aagtagataa taaaaaggtt ttaaataact gttttttcat
      661 caaaatcacc tcgaatataa gatacttaag aaaacgcttt ctttatataa ttagaataat
      721 caggaaattt aatgttgtca agagggttta ttttataaaa gctgttatat taaaggttag
      781 tagacataat gtacactatg ccaagtagcc cctctaccaa tggatagaga cgctcacttt
      841 gtgatttcta attggtctag cttaaacact tcagccaatg cttgatttta tgcacttttc
      901 ttttttcatt tctttgtgca ttttgaccgg tcatttttta gagatttgtt atttttgtga
      961 gcatttgata tgctcttttt atacctcaat ctcttctttt ctaatgttaa tgaataggat
     1021 aatctcattt ttagctgctc gtgagagcag tctttttttg tttcagggta cgtcctattc
     1081 tttggatttc cgcgctatta aataaccgat acttggggat tggggtagcg ccccaaaata
     1141 caaaaagaag aagttgcata agcggatttt tctttttttt cgcaagttgg taccaacttg
     1201 caaatcttgc tggaagactg atccctaaaa agaaatcgag gaattttttt agggatcggt
     1261 ttcttttttt atctttttag aaagtttgtt gggtcaaaat ttccagaagt aaaggtttga
     1321 ttggtttctt tttgacgatt tttgggttga tttcgtcggg aaggattgag ttgattttca
     1381 attttttctt tcacttcttg atacacttta gaatttgtac cgataagaac accagtatct
     1441 tttagatata aatcagcaaa ttctgccatg atttctgtca tgatagaaat ttcttttccc
     1501 atggcattga gttttgcttg gttactggtt gtgattgtat tggtatcagg ttcataactc
     1561 atacataaaa aacgaatggc ttctgaataa ttgttgacgt ctggattttt ttcgatgatg
     1621 gaatctaaaa cgtcaacgtg actgggataa agggttacgt gtttttgaac agaagttttt
     1681 ctcatgttat attcctccga taagtgtttc aatgagttgg ttgtttgtgt gaacatagtc
     1741 attcagtatg ttcgtatttt ctgtttgaat ggcaataaat ttttcgagta ataaatcgaa
     1801 ttgatctttt ttgtaattca taagttgacg gtcaaaatca ctttcgatta gaacttttag
     1861 atatttattt cgactccatt ttttgcccgt tttggttgtg agttcctgac aacgtttatc
     1921 tattcttgca ataattttgg gattgatatt tcgaactaaa atgtccatgg aaattttcct
     1981 ttcgtatcag cgtataattt atgtatatca tacaaatata gtatttgtat agctattttt
     2041 cgccaaaaca ctatacatag cctagccaca atggcaaaaa agtgatagca aatttttttc
     2101 tgagaaacgt gataggcaca gcctatctag caccttttgt aagaaagtga taccacgttt
     2161 ccttatgctc tttcaaaatt cagaaaaatc agctgtaaaa cgagtgcagt ccgagtaaga
     2221 ttcaacgaaa aaacggggat tggggcgcca ccaaaatatc gcaacatggg tccatgttgc
     2281 tctgctcgca aaagcaaaaa gaagaataaa accagcaatt taaattaata aaaattgctg
     2341 gttttattgc cagaattgcc accatttttt ctccgtcttt gattgttttt tctgtcataa
     2401 aatctaaaaa cccctcaatc tttgaataag ttttgaagat tgtctaataa gtcttttttt
     2461 agttctgaag gaatatcgga atttatctct gatttttgtg cattattttc tttattttgg
     2521 gtattttgtt ctctttctaa cttttctaac tgttgtaaaa gttccaattt tcgcttttgt
     2581 tctttgattt tttcttgtaa tgaagtaggt gctttttcag attgaacttg ttggttagct
     2641 agttctaatg ctttgttttg ttcttctttt gttggggcat gtttgttaaa gtcgtctgat
     2701 ttttttatct caggtttaaa tgtaaattga tagccaatga caggctttcc ccgtcctttt
     2761 ccgtatttct ttttgattgt taaaccttta aagattgcag ttagttcttg tcgtattgga
     2821 ttgaacacac gttgatcaat cgtaccttgg ttgtaacttt taggaatatc aagtaattca
     2881 tgaaagtctt cttttgaaaa ataagcgaaa cctttggttc tgtattgttt tagtaatcga
     2941 aacattgttt ttgaataact gctttctaat tcattgaatt gttggagaga atagcgcacc
     3001 cattcttcaa gattattaag taagggtaag gctttttcat gaacttgtat ttcagcaaaa
     3061 ggaatatcag attttccgtc gattttaaat tgattaaata aaacaaaacg ttcacgttgc
     3121 aaaccgtcag cacttcttct accaaaacgt aaatccatta atttgtcata cgttgtttct
     3181 aaatcatcta tgaatcgaac atttgcagtt gctttataat tgcttaaatc ttttaattgc
     3241 tcgaatgtaa attgtacttt tttatcacct acatcacgca ttcttgatac gatagaaaag
     3301 aacaaattca tttcgactgg tgtaaatttt cgcaacggaa ttgtatttaa ttcatgatga
     3361 tactttacga tttcattaga catatttaaa atagctcctt tcttactcgc acaagagata
     3421 cacgttatga aatacaaaat gcctatttat ctacatctta tattaacatc gtcgatgtac
     3481 taattcaatg tacaaacaga caaaacgatg tagataaaca gacaaaacga tgtagataaa
     3541 cagacaaaac gatgtagata aacagacaaa acgatgtaga taaaagcgtc attcccttgg
     3601 gagagtaagg atatagccga cggtaaagag gtttaaaaag aggtttaaaa agagattata
     3661 aaagaggggg acaaaatttc ccgcctaaaa aaccacttct tttttctcca aacagattcg
     3721 cgcatttttc tcaactggga atcttactcc ctacagtcgc ttaaaaaggt gtgatcgctc
     3781 tgctctcaca ttatactgcg cagtcgcttc cggctccttt gctaaaaata ttcataagga
     3841 actctttgag aagctatgag ccttcgtttg agacgtttta agacagtctt agtataaatc
     3901 ccttatcttg ttcttaaagc gccttacagg cggtcagggc aagccctaac aaccctaaag
     3961 ttgtctgaat gccctctaaa attcgattta agcactctta aacctattct tatatattta
     4021 tatacttaga cgtttcaaac gtcttaaaac gcaaattaga gcttgataaa gatatttgct
     4081 cgtgtgccga cttttttaaa agtctaggca cacgccatga caaagctgaa ataaactgct
     4141 tcagctttgt catggcggat tcgctcactt gaaaaagtgt tcatttcgct cgattattag
     4201 catatatttt ttaacttttt ttgctagcca aattttgcat tagttcgctc ataaatccca
     4261 ccctttcagt acgtcctacg gacgtatcgt tcgtaatgtg aaagtacctt taaaggtact
     4321 ttctacaaaa ctcactaaag ggtttggcaa gctctatatt tgctagccaa atacctacgt
     4381 atttggtgca aataaccgct tgtcaaaggg gcgcaagctc ccccttgaat cccccctcaa
     4441 aacccacgct ccacaataca aaaaaggcta gcaaatgcta gccacttttt gtaaagtttc
     4501 gcttgtttca ccttcggtga aattgttgct ttgcaacgat caggtagagc tacatttttt
     4561 ttcgttgttc aaatattctt acgccattta ctattaaaat cgtaggtatt ccaaagtaaa
     4621 gaaaaaattg attagcaggt aatacaaaat ataaaataat accagcaata attaccaaac
     4681 ttatagtaag ttttgttaat ttcatgaagt atgccacctt atcttatata gatatcaaat
     4741 agattaatat gccgattgta ttataaaatc catgagcaac tatggaatac catatatttt
     4801 ttgtttgtat ataaacaaaa cttaaaataa taccaataaa gaaaatactt aggcctcccc
     4861 catgaacaat agaaaacaga aaagaagaaa ttagtattga aatatattgt tgttttttgt
     4921 ttgcttgagg atcaatcata caaaatcgaa aaattccttc ttctaaaaaa gctccataag
     4981 tagcagcatt taaaaagaat aaaaggagag aaccatttac ttcattagga tttatacctt
     5041 ctgaaaaaga tgtatttaga atattgatta ggaataaaac agagaatg
//
LOCUS       DANKHL010000191         5017 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000191 DANKHL010000000
VERSION     DANKHL010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.201"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..165)
                     /locus_tag="PW101_002420"
     CDS             complement(<1..165)
                     /locus_tag="PW101_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2514793.1"
                     /translation="MSRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFK"
     gene            complement(367..762)
                     /locus_tag="PW101_002421"
     CDS             complement(367..762)
                     /locus_tag="PW101_002421"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDL2514794.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            complement(772..1620)
                     /locus_tag="PW101_002422"
     CDS             complement(772..1620)
                     /locus_tag="PW101_002422"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDL2514795.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            complement(1635..2462)
                     /gene="panB"
                     /locus_tag="PW101_002423"
     CDS             complement(1635..2462)
                     /gene="panB"
                     /locus_tag="PW101_002423"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDL2514796.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            complement(2474..3334)
                     /locus_tag="PW101_002424"
     CDS             complement(2474..3334)
                     /locus_tag="PW101_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HDL2514797.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKKLGN
                     STVIVSKENKSLYHAASVVSSNLVLGLINTSVTYLIQCGFTEKMAIEALYPLIEFNIR
                     NIKEKGIIESLTGPVERCDISTIKGHCEVLSDEDRALYMLLSKNVLEIAMLKNINRDY
                     SELEKYLGES"
     gene            3572..4741
                     /locus_tag="PW101_002425"
     CDS             3572..4741
                     /locus_tag="PW101_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL2514798.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT         1611 a          942 c          633 g         1831 t
ORIGIN      
        1 tttgaatgag cccgttgatt gtgcttgctt catccattta tcgaaagaag aaggcgtaag
       61 ctcatactct cgaataattt ctgcgcgagg cttaccagcg agatagagat cgacgatttg
      121 ttgtttgaat tcttttgaat aggttcttcg ttgacgtctt gacatgaaaa atcctccagt
      181 gtgtttttta ttattctaca caccttattt tttctgtcta gtctagtgta gccgattcac
      241 ataaaatgga ctttgtctac agtctgtatc ttctcttttt gtgtctactt tattgacatc
      301 tatccaatta gtaatcaata aatatttcaa taatagtcta gctatatata aataacatta
      361 caaaacctaa agaattatat ctgataacct tccatgtttt tcatatgatg ttactctttc
      421 aactttatta ttatcatcga caaaaacaac cttaggtcta tgatccttaa cctcattttc
      481 atttaattca caatatgcca ttaaaattat tttatctcca gttgatacac atctagctgc
      541 agctccattc aagcaaatca tccctgttcc tctctctcca gctattgtat aagtttcaaa
      601 tctatttcca ttattaacat caactatttg tactttttca tattcatata ttcctgctgc
      661 ttctaataaa ttcatatcaa cagtaatact tcctacataa tctaactcag cttgttcaac
      721 tgtagctcta tgtatttttc cttttaacat tgtaactttc attatagccc cctattgtaa
      781 ttcaaatgta aaattatcta ttaatcttgt ttttcctata aatactgcta ttgcaactaa
      841 aactgatttt tcaatataat ttactctttg taatgataaa gaatcaacaa cttctacata
      901 atcaatcttt gccaaattat aagtatttat atttttactt attatctcaa ttatttttaa
      961 gctgtctctc tctccattat ttaaagcttc cttagctaaa gttaaagatt tatttaaaat
     1021 agtagctgaa gatctttcct ctaaacttaa gtatgtgttt ctagaacttt tagctagtcc
     1081 atcctcttcc cttataattg gacaccctac aacgtctata tcaatattaa ggtccctaac
     1141 cattctttta attacagcta actgttgtgc atccttttct ccaaaataag ctctatctgc
     1201 tggtattatg ttaaataatt tacttacaac agtgcaaacc cctctaaaat gtattggcct
     1261 actagctcca cataaacctt ctgttaatcc agttatatta acaaaagtac ttgcattgtc
     1321 acaatacatc tcttcaactt caggattaaa tattgctgtt gctctacctc ttgtgcatac
     1381 ttctatatct ctttctaagt ctcttggata actacctaaa tcttcatttt tcccaaactg
     1441 agttggattt acaaagatac ttacaacaac tttatcattt tcttttgatg cttttctaat
     1501 taaactttca tgaccttcat gtaaatatcc catagtagga actaatccta cctttaagcc
     1561 ttgttttttc cactctttta cttgacttct aacatcattc actgttttaa atataaccat
     1621 atttcttgac ccttttaata taatttttct aaaacatcat cacttatttt aaacccatgt
     1681 tctggacctg gaaaaactcc atcttttact tcctctatat attttgtaaa tgctttattc
     1741 atctcttctg ataaattagc atatttcttc acaaatttag gggtaaaatc actatacatc
     1801 cccaacatat cttgatatac aagaatttgt ccatcacacc ctgcacctgc tccaatacca
     1861 attgtaggta ttgatatctc tttagaaatt aactctgcaa gcttagttgg aacacattct
     1921 aacactacag cgaatgctcc tgctttttct acagctttag cagctcttat taattcttta
     1981 gcagcttcct tgtcctttcc ttgtacctta aatcctccaa atgcattaac agattgaggt
     2041 gttaaaccta tatgcgccat aacaggtata gaagccttta ctatagcctc tattttatcg
     2101 catacttcta tccctccttc taatttaaca acctgagctc gtccttcttt tattaatctt
     2161 cctgcattaa ctactgaatc atatactgaa gtttgataag acataaatgg catatctgca
     2221 acaactaaag tgttcttagc tcctcttgta actgctctag tatgatgaat catgtcttcc
     2281 attgttactg aaagtgtatc ttcatgtcct agacatacca taccaagaga atctcctact
     2341 aatattccat ttatcccagc ttcatcaatt atttttgctg ttgaatagtc ataagctgta
     2401 agcattgtta atttttcatt tcttaattta gattctttaa atgtaactgc tgtatttttc
     2461 atctatttct ctcctaactc tctcctaaat acttttctaa ttcactataa tctctattaa
     2521 tattttttaa catagctatc tctaatacat tttttgataa tagcatatat aaagctctgt
     2581 cttcatctga taaaacttca cagtgacctt taattgttga aatatcacaa cgttcaactg
     2641 gccctgtcaa actttctata attccttttt cttttatatt tctaatatta aattcaatta
     2701 atggatataa agcttcaatt gccatttttt cagtaaatcc acattgaatt agatatgtaa
     2761 cgcttgtatt aataagtcct agcacaaggt tactagatac tactgatgcg gcatgataaa
     2821 gagatttatt ctccttagat acaattacag tactattacc taactttttg aagaaatcac
     2881 ataaaaactt tgcatatttt tcatcgcctt caattgttat aaaagatttt tttaattttt
     2941 tgtatgaatt atatttatct gatattgaaa acataggatg gacagaatag ccataagcac
     3001 catagtcctg tatatttgaa aagatatctg aagatattgc accactgcaa tggcagatta
     3061 atttgttttt gatttgataa tttttaattt cttgccatac ctcctgaatt tgaccatcag
     3121 gagtcgtaat gaaaatagta tcattttcat ctactaattc tcttaagttt aaatattgtt
     3181 tactagatgt aaaattcgaa gctgctaatg acgagtcttc gcttttacta taaaatcctg
     3241 ttacttgtat tttatgttcc tggaaatact tcccgaatga acaacctacc ttgccggctc
     3301 caataaaacc aacgttcata attatcacct ccataaagaa gtgagactac aagtcccatt
     3361 cttacgatat gagcttaaaa ataaaaatta aatcaaatat acagtatgtt tttttcaaat
     3421 atcatccata gttaatttat cataatatcc catattttaa aataaaagat agcgtaaaat
     3481 attttgtgta aatagaaatt tagggtagaa aaaaaagtcc attctgtaaa attaaagtta
     3541 ccacacagaa attttatagg aggactttct catgactaaa tttactatag aaattatgca
     3601 aacactactc aataaagggg atttagatga attatttcag gatcatttag agcgagcgat
     3661 taattcactt ctacaagcag agctaactgc ttttttagat tacgaaaaat atgatcgaaa
     3721 tggattcaat tcaggaaatt cccgtaacgg gaattattca cgtacattta aaacagaata
     3781 tggcgagttg aatttaacta ttcctagaga tcggaatggc gaattttctc aacaaacact
     3841 accagcatat aagcgaacca atgattcatt agaaacgacc attatccaat tatttcaaaa
     3901 aggcattact atggctgaaa tctccaaatt aattgaaaaa atgtatggtc accactatac
     3961 gccacaaaca atctccaaca tgagtaaatt ggtggctgaa gatgttttag cttttaaaga
     4021 aagaacgtta gaagccaatt attccgttat atttatggat gcgacgcata tccctgtgaa
     4081 gcgacagact gtttcgaagg aagcagtgta tattacgatt ggtattcgtt tggatggaac
     4141 caaagaagtt cttgggttta ctattgcccc aactgagtct gcttatattt ggaaagaagt
     4201 tcttcaagat cttagaaaac gtgggttaga agaagtttta ttagtagtga cagacggatt
     4261 aagctgtatt gaagaaagta tccatagtgt gtatccgaat gcccaatttc aacaatgttg
     4321 tgtgcatgta tctagaaata tcgctcataa agttcgtgtt cgaggtcgaa aagaaatttg
     4381 tgaggatttc aaattggttt accaagcgaa ttcaaaagaa gaggcattgg atcacatcga
     4441 ctttatgact aggaaatgga aaaagcagta tccaagagtc gtcaatttac tcttgaatcc
     4501 tgccctatta accttttata atttccctca cgccatcaga cgaacaattt attcgacgaa
     4561 cctgattgaa ggctttaata agcagctaaa acgatatact cgaagaaaag aacaattccc
     4621 taatgaagaa tctctagaga gattcttcat ttctcaattt aatcaatata accaaaaatt
     4681 tttaggtaga attcacaagg gatttaaaga aattcaggat acattagagt cgatgattta
     4741 actgtaatta acagaatgga ttttccattt acacataatt cttgacgcta ccataatcta
     4801 aataaccatc atattcatct actacttata taattgagtt ttattaaact ttttaagttt
     4861 ctattctatc aaatatttaa acaatctctg catccgaaaa gactacctcg tatagtttta
     4921 ctaacaatat ttttattagc gtgttcaaat cctgatttct tgtatcaatt attttctgtt
     4981 cactttcaat aagaaaaaag tctttacttc catggat
//
LOCUS       DANKHL010000192         4985 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000192 DANKHL010000000
VERSION     DANKHL010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.202"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..182)
                     /locus_tag="PW101_002426"
     CDS             complement(<1..182)
                     /locus_tag="PW101_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2514799.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="PW101_002427"
     CDS             517..951
                     /locus_tag="PW101_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2514800.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="PW101_002428"
     CDS             974..1294
                     /locus_tag="PW101_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514801.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="PW101_002429"
     CDS             1435..1626
                     /locus_tag="PW101_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514802.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="PW101_002430"
     CDS             complement(1697..1954)
                     /locus_tag="PW101_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDL2514803.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="PW101_002431"
     CDS             2112..2375
                     /locus_tag="PW101_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514804.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="PW101_002432"
     CDS             2399..2587
                     /locus_tag="PW101_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514805.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="PW101_002433"
     CDS             complement(2670..2855)
                     /locus_tag="PW101_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514806.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="PW101_002434"
     CDS             3070..3441
                     /locus_tag="PW101_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2514807.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="PW101_002435"
     tRNA            complement(3591..3663)
                     /locus_tag="PW101_002435"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="PW101_002436"
     CDS             complement(3709..4137)
                     /locus_tag="PW101_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514808.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="PW101_002437"
     CDS             complement(4197..4955)
                     /locus_tag="PW101_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDL2514809.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1737 a          782 c          977 g         1489 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctt
//
LOCUS       DANKHL010000193         4956 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000193 DANKHL010000000
VERSION     DANKHL010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.203"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(55..828)
                     /locus_tag="PW101_002438"
     CDS             complement(55..828)
                     /locus_tag="PW101_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2514810.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            972..1322
                     /locus_tag="PW101_002439"
     CDS             972..1322
                     /locus_tag="PW101_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDL2514811.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1303..2019
                     /locus_tag="PW101_002440"
     CDS             1303..2019
                     /locus_tag="PW101_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2514812.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2019..3467
                     /locus_tag="PW101_002441"
     CDS             2019..3467
                     /locus_tag="PW101_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2514813.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(3537..4046)
                     /locus_tag="PW101_002442"
     CDS             complement(3537..4046)
                     /locus_tag="PW101_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514814.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4036..4761)
                     /locus_tag="PW101_002443"
     CDS             complement(4036..4761)
                     /locus_tag="PW101_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514815.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1703 a          843 c          998 g         1412 t
ORIGIN      
        1 ttaagaaaaa ataagcgatt ttcttaagaa aagaggtagc ttcaaaaaaa gatcttaaat
       61 cagatcaaaa tacttcaatg cgtggacaat tccgccttct gtatttttct ttgtgataaa
      121 ggtagaaagt tcttttaatc cgtcataggc gttgtccata gcgattttgt tgtcgcaagc
      181 ttccaacaag gcaaaatcat tcggaccatc gccaaatcca aaagtaggaa ctttttccag
      241 ctgaagttct ttttgcagac gtttgacacc tgcccctttt gagattcctt tcttcactgt
      301 atcgatcgag taaggaccat tccgataaaa agttagctca ggaaaatgtt cgtgatagta
      361 ttcgtcacct gattgagata agatcagcaa catatttact gtgttttctt taaagcctaa
      421 aggatcaatt tccggaacat ctgaatgaat gaagtcgtaa gcattgatca ttgtttggct
      481 atgaccggta caccagattt tctgatcgtt atagtaagaa agttcatgac cgttcaactg
      541 tacatgatca tacatttttt gacattcttc tgaagaaaat gtttgagaat agatttcttc
      601 gccgtctact cgaatgaaag aaccattcat ggtgaccgca gaagtaatgc ccgcatcttc
      661 catgatagag tggatttctg cttctgtacg tcctgtagcg atgacgggga gaacattgtt
      721 ttcttttaat gcgttcatag cttttgccac ttcgggtgtg atttttgatt tcgcatctag
      781 taatgttcca tcaagatcaa agaaagtgat tgcacgataa tttgacacaa ataaaactcc
      841 ttttttattg gaataagttt atcttaacaa agaatgaaca aaagacaaac ctttcgtgaa
      901 gaattcctga acatattggt tcagtcaaca atttagagta tactataaga agagaattta
      961 ggagggaaag aatgattttt ttcagtattg gtatcttatt cttaataatt ggcagtcttt
     1021 ttcggattct gccctcaaaa ggaaatctgc ctttttatgg gtatcattcg cctcttgccg
     1081 caaaaacaga cgctcattgg cggctggctc aaaagacaag cggaaactgg ttttttctta
     1141 tgggcttact gatggcattg atcggctatt atttaaagac atctgggcat acgaattatt
     1201 ttctgattga aatgcttctg ctagtatttc caattatgcc tatttttatt atgacagaaa
     1261 agaaattaca gaaatatgac ctggaaactg gagggaacga taatgaatat tttaatgatt
     1321 gaagataatc aatctgtttc tgaaatgatg cagatgtttt tcttaaatga aggttgggaa
     1381 gcaactttca agtatgatgg aaaagaaggg ctcgatgcct ttttagaaaa cccacaacat
     1441 tgggatatga ttacactaga cctgaatttg cccacactgg acggtatgct agtaggaaga
     1501 gagatccgca aagtttcaag tactgtgccg attatcatgc tgacagctag agattctgaa
     1561 agcgatcagg tgatcggctt agagatgggg gcagatgatt atgtcacaaa acctttcagt
     1621 cctctgacgc ttattgctcg aatgaaagct ttgcatcgcc gtagtgagtt ggcagaagca
     1681 agagaagaag cagcagtttc agatgatcat tttgatatcg agactagtca tttcaagatg
     1741 aatacgaaaa cacgagaagc gtatctggac aaccggccaa ttgaaggact gacgcctaaa
     1801 gagttcgatc tattgtttac tttagcaaaa aaaccacgtc aagtattctc tcgggagcag
     1861 ttgcttgaat tagtctggga ttatcagtac tttggtgatg aacggacagt ggacgcccat
     1921 atcaagaaat tacgacaaaa aatcgaaaag gtcggaccac aagtcatcca aactgtttgg
     1981 ggagtcggct acaaattcga tgattctggt gttgcctaat gcgttatttg tatcagcaac
     2041 tcttagcttt ttgcggaatg gtcgcgttga tcttattgat cgttggagtt tcttttacac
     2101 aactgacaaa acaaacgatt gaagaaaaca attaccagca actgtttgga tatgctgaat
     2161 cagttgaaaa gacaacgcaa acctatgccg atacgctccc gcagtttagt caagatcagg
     2221 cgtttcaaaa tgcattgctg ctgacagagc aagtactgac agaacaaaat gtgaacttta
     2281 tatttatcga taaaaacgaa cgtgtcattt atccgactaa tacagcttta ctgaatttta
     2341 ccttaacagc tgatcaatgg aaaaatctga ggaatggacg tcaagaaaaa ttcacatcga
     2401 ataaaaatat tttaggacaa gcacaggcga cctcttacgc acttgtgcca tttaatttga
     2461 atcatgaatt ctatggagca cttgtagtta gtcaacctgc gcgaaatatc gataacagcg
     2521 ttcgtccaat tacactgaac ctgttcaaag gatttatttt ttccagtatc gttgcagtta
     2581 ttgccagcta tgcttttgca gcattccaag ttaagcgtat caatcggtta aggaatgcga
     2641 cgaaagaagt gacgaatggt aattttgatg tacagctccc cgtacatgac aaagatgaat
     2701 ttgatgagtt agcagatgat ttcaataaaa tgacgaattc attgaaagaa tctcaagcag
     2761 aaattgaaga acaagaaaat cgtcgaagac aatttatggc agatgcttct cacgagatga
     2821 gaacacctct tacgacaatc aacggacttc tggaagggtt agaatacaat gcaatccctg
     2881 aaaatcagcg agaaaacgcg attaaattga tgaaaaatga aacagagcgt ctgatccgtt
     2941 tggtgaatga aaacttggat tatgaaaaaa tccgtacgaa tcaaatcagt atggtcatta
     3001 aaaaattcaa tggaacagaa acattgaaaa atattgttgc acagttagaa gctaaagcag
     3061 aagcagcagg agatacattg acattgaaag ccgttgatga tattgatgtt tacgctgatt
     3121 atgaccgatt tgtccaaatc atggtcaata ttattcaaaa cgcgatacag tttactgaaa
     3181 acggccaaat cacgattact ttagaaaaag gctatcttga aacgatcatc acgattgaag
     3241 atacgggaat tggaatgtct gaacaacaaa tgaaaagtat ctgggatcgt tattataaag
     3301 tagatccttc aagaaaaaat acaaaatatg gtgaatcagg attaggttta ccaatcgtgc
     3361 agcaacttgt acgcttgcat aaaggaaaat tagaagtaga aagcgaacta ggaaaaggaa
     3421 caaaattcgc tgttcgtttg cctgacgtag aaatcaaaaa agagtaaaaa acaagcggta
     3481 taaaagatca tgaatcgatc ttctataccg cttgttttta ctataaaatg attgatttag
     3541 cccgcgggct ctaaacgatc aatataagga ctttgagcaa tagtagcatc atgagtaaag
     3601 agtaagatac cttgattttc ttcatgagct aaataagaaa gcaagtggag tagctcttgg
     3661 atctccaata cactaagttc atcaaatgta tcttcaataa caatattgtt tcgcttggaa
     3721 agcaaggcat gaagtaattg cagctttact ttttcaaaag acgtcaactc agctgccgca
     3781 caattaagca aggaaggaga gatatcaagt ttttcttgat aggccaaacg atcttgtttg
     3841 atttcttttt ccggtacatc caacaggaca tggtctttaa gattcagata aggaaacatc
     3901 ggccaatttt tttggatgat cgcaaaatta tccagttttg caacaagaac ctcttttttc
     3961 aagtgctcca atattttatg ctgtttttcg agaggacaca aaataacggt caagtgttca
     4021 gcatgaatca aatattcagg tttcattacg tccaggcctc ctactttgca aatgatggat
     4081 aaaaaaagtg aaagggaata gactaagaat gagacagcat ccactgagcc caataagagt
     4141 ttgggcaaat cccttaagta tatctgtgaa aaaaagatca ttttggaaat cgatggtaaa
     4201 aaaagtctgt tgatccatcg gaattgtaag tccgcttacc gaagtcaaga tatcacggat
     4261 atctggcagt tcagtagtct ttaagacaag aaaattgatc ctttgaagaa gcgcttcata
     4321 aactgactgg aagctgacga tccacaataa aaccaagata caacaaagaa agagaggaaa
     4381 gaacagctgc cagaaaataa aaaggaataa tttggtttta gaaagaccag tcaggcgcca
     4441 attgatcaat tcctgcttgt tttgcctgac tgaatagtaa aagaaaaaga taaaaaggaa
     4501 ggcaaataga aaaaataaac tgaatatgcc ttttatataa aatcggctag ctaattctaa
     4561 agccggaaga agctctgtag atgcagaagc gccaataaat gaaagccgat cttggacttg
     4621 attggctaaa gccgattgcg aatggtaaag agtcaggcat gctatcaata aaaaagcaga
     4681 aaagcttgaa aataaacaat agagtattgt ctgctttttg taataaatta atcgagcagt
     4741 agcatagcgg attattttca tacttttcac ctctataaaa agtatagaaa atgaatatga
     4801 ataaagtatg aaattccttt agtgaaatca cgaattaaaa ccagaaaata ttttgtaaaa
     4861 agcaagaggt tgtgaagaag ctgccctatt ctattaccgt agatgcaagc ttttgaacac
     4921 tgtttattcg aaaagaggag cagcgacaat aatttt
//
LOCUS       DANKHL010000194         4825 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000194 DANKHL010000000
VERSION     DANKHL010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.204"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            185..1000
                     /locus_tag="PW101_002444"
     CDS             185..1000
                     /locus_tag="PW101_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2514816.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            1010..1672
                     /locus_tag="PW101_002445"
     CDS             1010..1672
                     /locus_tag="PW101_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2514817.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            1877..3541
                     /gene="alsS"
                     /locus_tag="PW101_002446"
     CDS             1877..3541
                     /gene="alsS"
                     /locus_tag="PW101_002446"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDL2514818.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            3554..4264
                     /gene="budA"
                     /locus_tag="PW101_002447"
     CDS             3554..4264
                     /gene="budA"
                     /locus_tag="PW101_002447"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDL2514819.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            4394..>4825
                     /locus_tag="PW101_002448"
     CDS             4394..>4825
                     /locus_tag="PW101_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDL2514820.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFL"
BASE COUNT         1521 a          765 c         1038 g         1501 t
ORIGIN      
        1 aaatcaaaaa aaataaagag atttacataa cttgttaaat aagcgcttac aaacataaga
       61 agaacggaaa tgaatcagct ataataaccg ttgttctgga ttgtaatcat tacaaagaaa
      121 tgaagattat aactttggag gtgttcgaaa tgaaagaaaa aaagcaaaaa ttcgttcaaa
      181 aaacatgccc tttattcaat ggttgggtat gattctgctg attgtattag ctttattgac
      241 gatcattcct tttttatgga tggtttctgc ttcattcaaa gcaaataatg aagtgttcac
      301 gattccgatt caatggatcc ctaaaacttg gcatttagag aattatctgg ttatttggtc
      361 tcgtattcct ttagtgacat tttttaagaa tacagctttt ttaagcgttg tgatcacgtt
      421 gattcagttg tttacttcta gttttgctgc ttacgggttt tctaaactaa attttaaagg
      481 gcgcgatatt ttatttttag catatatcgg cacgattgct gtcccttggc agtcttatat
      541 gatcccgcag ttcattatga tgagaaaagt gggtttaaca tatacgttgt ggtcgttggt
      601 gctattacaa gcgttcaatg cttttggtgt gttcttgttg aagcaatact acagtagtat
      661 cccagattct ttatgtgaga gtgcgcggat cgatggctta tcagagtggg gaatctatcg
      721 caagatcatt ttacctctta caaaaccggc gattgccagt ttgacgatca tcacattcgt
      781 taatacatgg aacgattata tggggccatt catttatttg tcttctacgg aaaataaaac
      841 aatccagtta gggttgaaaa tgtttattgg gctatttgat gcagagtacg cattaatcat
      901 ggctgcctca gttgtttctg ttcttcctgt tgcttttata tttttagcga tgcaaaaata
      961 ttttgtagaa gggatcgctt catcaggaat gaaggaatag gaggtgatta tgaaagagca
     1021 gcgttttgac aaaaatgttt atatggaatt cttttattgg ttgtatcaat cattggcaag
     1081 tagcctttgt ttttggtgtg tcaatatctt gtatatagca gctgcgttcc ttttgtcgat
     1141 agatggaagg aatgtctttt cctttatttt ttctttactt tttattggtc caggttgcat
     1201 cgtgatgata ggtatgacag aagaattagc aaaagaaaaa agtttttcac cagtacgttt
     1261 gttcataaag acgtttcgcc aattttggct aaagggattt ttttattggc tatttgcttg
     1321 gatagttagt gtgataatga tttttgattg tttctttttt atacgttttt cttatggaaa
     1381 atggttgatt ccgttgtttg ttttacttgc ttgtttatca gtttccttta gcatcaacta
     1441 ctggtatttt caggtgagaa atccagcgtc gaaaccaaat caagttctac ggatagcgtt
     1501 ctactatacg ttgaaaaaat ggtatgtgag tttactagat tttttactat tgacaagttt
     1561 gtttctcttc tttttcgtaa agccccaatg gtgtattcta ctaggtcctt ctatcgtatt
     1621 tggtttgatt tacttcaaca atcgtaaatt gatgagaact atggatctgt agactttata
     1681 aattgaaact ttcaattaag aatgacagtt tttatgtaaa aactgtcatt ctttttcatt
     1741 ttttgaaaca tatggctaaa caaatgaaat aaaacgtgct atacttttga atgaaacagg
     1801 tttagaaact gtatcaatta tttgtgatta tttctaacta ataattaaaa ataaagcata
     1861 aaaaggacga tgttcaatgg acgaaaaaat gaaacaaggt tcgaaatctg tagtagaaag
     1921 tttgattaat catcatgttg attatgtatt cggtatacca ggtgcaaaaa tcgatggtgt
     1981 atttaatgaa ttagaggatc aaggtcctga attaatcgtg actcgtcacg aacaaaatgc
     2041 tgcatttatg gcacaagcaa tcggacgtat tacaggagag ccaggagtag ttatcgcaac
     2101 aagtggtcct ggtgctagta acttagctac aggtttggta acagcgacag cagaaggtga
     2161 cccagtttta gcaattgccg gacaagtaaa acgaagcgat ctattgaagt tgacccatca
     2221 aagcatggat aatgcagcac tgttccagcc aatcacaaaa tacagtgcag aaatccaaga
     2281 tccagaaaca atttcagaag tgattgccaa tgcttatcgt atggctaaga gttcaaaaaa
     2341 aggcgcgagc tttatcagta tcccgcaaga tgtagtggat gcacctgtcc aagggaatgt
     2401 catcaagcca ttgagcgatc ctaaattagg ttctgcttct gcagatgata tccgttacct
     2461 agctgaacgc attagagaag caaaactacc ggtactatta gtaggtatgc gaggatcaag
     2521 tgaaaaagaa acgttagcca tcaggcaatt agttggaaag acagcattgc ctgttgtaga
     2581 gacattccaa gcagctggag tgatctcacg tgaattagaa gcgcatttct ttggtcgtgt
     2641 gggtttattt agaaaccaac caggagatat gcttttgaaa cgaagtgatc tagttattgc
     2701 tattggctat gatccaatcg aatatgaagc aagaaactgg aatgcggaaa aagatgcgcg
     2761 gatcattgtg atcgatgaag cgccagctga aatcgatcct tttatgcagc ctgaaagaga
     2821 attgatcggt gatatctcag ccactcttga tttattgaca ggttcattag agcctcaaca
     2881 agtgtcagaa gatgcaaaag aatatttagc atctcttcaa gcagaattaa cggagcggga
     2941 cattgttcaa tcaaaaggag aagcaggtat cttgcatcct ttagaagtta ttaacacgct
     3001 tcagtcaaaa gtaacagatg atatgactgt taccgtagat gtaggtagtc attatatttg
     3061 gatggcacgc cactttagaa gttatgaacc tcgtcatttg ttattcagta atggtatgca
     3121 aacgcttggt gttgcattgc catgggcgat ttctgctgca ttggtgcgtc caaatacaca
     3181 aatcgtatca gtttccggcg atgggggatt tttattctct gcccaggatt tagaaacggc
     3241 ggtacgtaaa aaattgaata tcgttcattt gatttggaat gatggacact acaatatggt
     3301 agagttccaa gaaaaaatga aatatcagcg ggcgtcaggt gtagactttg gtccagttga
     3361 ttttgtgaaa tatgcagaag cttttggcgc taaaggaatc cgagctacaa gtgtagagga
     3421 attagaaaaa gctttagaag aaggatttgc gacagaagga cccgtaatta ttgatattcc
     3481 gattgattac cgtgataatg agaaattggg agaaacaatc ttaccagatc aattctatta
     3541 aggaagtgtc gacatgactg aaaaaatctt ataccagcac gggacattag gagctttgat
     3601 ggctggcttg atggatggaa cagaaacgat tgctcatata ttagaaaaag gaactctggg
     3661 attgggaaca ttgcatgggc ttgatggtga agtcatcttt ttagatggcg tagcttatca
     3721 aggaagatca gatggctcag ttgcccaatt agatggaagt gaacttaccc cctatgcagc
     3781 aattacagat tttacgccag atactagttt ttctgtctca aaaactgcag atggagaaca
     3841 attgaaaaaa gacatattag taagagaagc cggggaaaac ctatttttgg cagtgaagat
     3901 aactggatta ttcaaaaata tgcacatccg tatcatgccg aagcaagaaa aaccttacag
     3961 aagactagca aaaatttcag agtctcaacc agaattccaa caaagcaatg tccaaggaac
     4021 attagtcggg ttctttacgc ctgaattatt ccaaggagtt gctgcagcgg gatttcacct
     4081 gcattttatc gatgatacac atacatttgg cggacatgta atggattttg aagtggctga
     4141 aggaaaagtt gagattagcc gcatctcgtc ccttgtacag cattttccag ttgatgatcc
     4201 gacttttgtt cacgaaacga ttgattatgc tgatttagca gcagaaatcc aacaagcaga
     4261 atagaaaaag acagcgagat gcgttcaagg tgattaacct gacgtatctc gtttttttga
     4321 ggagaaaacc tagcgtttac aaaaaaactg agctataatc ttctccatag atcaaaaagg
     4381 agcggttttt atcatgtgga cagaaaaaca gaaaaaagat gcatacgaaa ttttattatt
     4441 acagcaacaa ggattattag aagcagaaga taactggtta gcaaatttag caaattcatc
     4501 tgcattgttg aatgagactt tgcctgagac tgtctttgca ggatattatt tattcgatgg
     4561 taatgaattg attttaggac cgttccaagg gcgagtttct tgcacacgta tcaagatggg
     4621 aaaaggagtt tgtggagaat cagctgaaaa acaagaaaca ttgatcgttg atgatgtcaa
     4681 aacccatgca aattacattt cctgcgattc agccgcacaa tcagaaatcg tagtgccaat
     4741 ggtaaaagaa ggtaatttaa tcggtgtttt agatatcgat tgcggagtaa cgaaagggta
     4801 tgatgaaatc gatcaagcat ttcta
//
LOCUS       DANKHL010000195         4809 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000195 DANKHL010000000
VERSION     DANKHL010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.205"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(91..1176)
                     /gene="xerS"
                     /locus_tag="PW101_002449"
     CDS             complement(91..1176)
                     /gene="xerS"
                     /locus_tag="PW101_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HDL2514821.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3252..3770)
                     /locus_tag="PW101_002450"
     CDS             complement(3252..3770)
                     /locus_tag="PW101_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HDL2514822.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1580 a          786 c          729 g         1714 t
ORIGIN      
        1 tataaaaatg ataatgaagc ttgcctttaa agttgggctt ttatctaatt ttagagacag
       61 gcttcattat tatactattt tttactacat ttacaaagcg ttcaaagcat caattgtagc
      121 tgcattgtct atatgagtat ataaagttgt tgtatttgta cttgtatgtc ccaattgttg
      181 ggcagttaat accaatgaat ttgtctgttg atagagtcta gtagccaaag tatgacgtaa
      241 tttatgtgga ctgacttgga ctctgaatcc ttttgaatat tttgctacca tccgttcgat
      301 tgcaataccg gatatacgct tggcttctcc ttgataaatt gctagaaata aagctttttc
      361 gttttctgtt actttgtatc gttctttccg aatagctaga taattagcta aatattccat
      421 tgcaaatgct gctatattta ctgcgtctct cttatttcct tttctagtta ctaccacatt
      481 attcttttct aaagataggt cctctaaatt aatatttaca acctcggaga gtcttaatcc
      541 agatcctaaa atcaatgcat tgatcgccag gtctctttct acatctcgtt gataatagat
      601 caaagctcgt ttgctcaaag tttttttata ttcattggca atataatcta gataattttg
      661 tgtatcttcg tctagaaata attttttctc gattgcgttt gcacgggcag ctaacgtagt
      721 tttatctgtg atagttgcta cttgatccat aggattattt tctaagtatg gttttcctaa
      781 aggattattg gaacttttcg aaagatattt aaatagaacc tttaatgctg tgatatttct
      841 attgatagta gaagtagata gacctaagtt attattatct ttgagcttag tttttgttgt
      901 ctcttttttt acattttcct ttgtgcgacg tttcaaatga gatttgtaca agtttagatc
      961 ataggcagtt aactgtctta tcgaggtgat tgaagtatcg ggaacgatag actcactcag
     1021 aagccattca taaaagagct tataattttt tagatattct aaagtagttc gaggtgattg
     1081 atctttatcc tcacgatctt gaacaaaatc ttttatataa tcgggcatat gaagtatgat
     1141 tgtatctatt ttttgatata tttctgtatt tttcatgtta ttcatccttt cgataagtag
     1201 gggagaggag tatctctata attggactat accaattata gagtgttttg tcaattggta
     1261 tactctgaaa tttattaatt tatttcaaaa aagcagataa ataggtatag atttaataat
     1321 ttaaaataaa tggtatacat gaataggaaa ggaaacagaa ggaggcaatc ttaatttttt
     1381 aaattgtcta aattatatag aaagaacaat ctttctatat aattagacat aagagagact
     1441 acggttgatt tgatattaac aagcgtatat gaatgtatat acaaataaaa aaggggaggg
     1501 agaagttaaa gaaaattcag agaagtgggg cactcagtag tattaacaat ttaaaaggaa
     1561 tttcttaaag aaaatgggtt gcatcctgga gatacaattt ttttagataa agaaaaaatt
     1621 caagcaacaa ttcaaaaaaa tcttaactgc tgagattgat gtagcaatat ctcaatcatc
     1681 aacaaagtac gatcctgcct ttcaaaagct tattgataaa tgtttattca ttataaaaat
     1741 aaaagaaaaa tttatgtcag cttttttcat caaagtggta atgataattc gtctattaat
     1801 tgtatttttt aggaaatatg gtataattgt tttaacgaaa aggtaattca tgatacacca
     1861 aaaaccacta gctggcgggc tagtggtttt cttttacgtt ctattcagat taacgggtag
     1921 gcttactgtc ttttttggca ataattttgc aatcaagcca tttacagatg taatgcgcgg
     1981 atacttctgc taagattgca aaaaacatat cgaaaaggta attcataata caacctcctc
     2041 cccttgccat aataggccgg ggtagcagac agcataaata ttataccata actgaaaatt
     2101 tcattttagt acatttcaat atattgatga accagcacaa taaaacatgc catgactaac
     2161 agaaaaaaac ttattaaacc agatataact tgtaaacatc caactatata gaaaacgtaa
     2221 attttctata tagttggatt aagataacga ctatttattt tatcatattt ttactatacc
     2281 ctatactttt acttggtata tttatgaata atcgcaaatt tacccctata ctactagttt
     2341 gtaagttaga ttaataaaaa tactaaagtt atttgttctt ttattaaata cacctacaac
     2401 ttataaattg atggtattta gataacacga aagaataaaa ataacagctg atagtaaagt
     2461 ttgttgacga ttgctactca atcgaaagtg agttagtgcg tatgaacttg cttctgaaaa
     2521 cttatgaaat gaaagacctt ttacatcttg cagaaatcgt tactgggatt aaactaagca
     2581 ataaaaacta atgttcccct cattctttga acttctacta tttgatttat ttattctgat
     2641 aaatgacgat ttcaccttca ggatcttcaa atcccgtata gaatttattt aagatctctt
     2701 cagtatcagt aataggatag gcgataggcc acataccctt tgatggttag aataaactgc
     2761 tcaaaatctg tatcgtatgt tggctttagc atattaacaa aggctatctc caaattataa
     2821 accataagcg ttgaatcgtt cttaacaatt tcaattcttc ctaacccaaa gtcagaagat
     2881 ataattaccg gacttgcctt acccttcatg tgggttgttg agattccata tttaaaggga
     2941 attgtcggta ctatcggcgg tctgaaaaac atcccaaaaa taaaaaaatc tttcccctgc
     3001 tagtctccta agtccctttg tagcggatat ggcaccataa tctcatttta tcgaagtgag
     3061 tcatggttta tctgttggcc aaccaaaatt gatacaatct aacgatgcac cctctacggc
     3121 attctagggg agtgcacttg cattcaccca ataaaaaccg gcagttagca aagaacttat
     3181 gtagtcgtat gttcttactg ttaacataca agaggtctcc tttccacaat tgcttagcag
     3241 taaacgggaa tttacaaagc taatctcttc cattttctta tctttgttct aaaagtccca
     3301 aatggagcaa ctgtatttac atgaataaat ttatatactt cccatacagc cgttttggtt
     3361 gcctcgtctg cccattttct tttgtgtgca gtgaatagat cttcatcact cattgaatca
     3421 atcatcatgt taatatcatc aatattacga ttgagaattt cctctaattc tttcaatggt
     3481 agatgtgcat aggtatttgt aaaccactta tataactctc ctaactgatt ccatttaaat
     3541 tgatcagaag gcgttttaac agataatcca tttctttcat cattttccca ttttagaatt
     3601 aaattcgtcc aaccaacttg atatgctaaa ttttcagcag gtgttctatc cacttcctca
     3661 actcttttat ccttcaattc ttcaggaata gtttcaaatt ctaaaatata tttagtataa
     3721 gtcctattta tttctttttt taattcttct ttatttttat atgttctcat tatattacct
     3781 ccataaattc ctaattgtct cctttaattc atctataatt tcatccacga tattttaatc
     3841 cgttctacaa tgcatatctg atatgcattg actgtctaac tcatatgtga atgatttata
     3901 aaatatccca acgtctcatc taaagaaatg ttgatcgtca gcaaggagct aaaaaatacg
     3961 aatatatttc ttttgtagta aaatcggact ggtttgcata tcattttctt cgtaaatatc
     4021 aaaatcatgt taactactat caattactaa taaaaactac cactttaagc gttaagaact
     4081 gttaagttta gttaagtgag gatagttcac aaaatccatt gttaaacttc acgaaagtgg
     4141 taaaactcaa aatgctttat ataggattac agcatcgctc tttcttcgat tgcgcgttgg
     4201 gtaaaaaata ttatgaagta aaaattgacg atgacacgtt ccttacggct catcagatga
     4261 aacagctaca aaaacgaaat gatcaactcg aagaaaattt aattctaaaa aagcgattgc
     4321 catattcacg cctcgctcga cgaactatta aaggtcaaga tttacctgaa gatcatcctt
     4381 tttaggaaat tgaagctttt tatgttgcac aatgcattta taatactaca atgatgtttt
     4441 caccagatat tcttctaggg tgcggtgtaa tgaaacagat acacttgcaa aagaaaattc
     4501 accgcgaatt tactaaaatt atgaataatt atgttaatct tccgaaattt ttttccaaaa
     4561 ttaagggatc aagctggtgt tatagattgt ttaaggttgg caaaaaaata acttcttttt
     4621 aaacaattat agaaaaccga tactctgtta agttggttga cacggtgtcg atttttatga
     4681 ctttcttatt ctgaaacttc actaacggac agtacctaaa aagtctatag tatggacgtt
     4741 tacgagtact attttatcaa aaatgctgct catttcaact tatatgtttt agtgaactac
     4801 ccccactta
//
LOCUS       DANKHL010000196         4757 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000196 DANKHL010000000
VERSION     DANKHL010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.206"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(137..1099)
                     /locus_tag="PW101_002451"
     CDS             complement(137..1099)
                     /locus_tag="PW101_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2514823.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1101..2540)
                     /locus_tag="PW101_002452"
     CDS             complement(1101..2540)
                     /locus_tag="PW101_002452"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDL2514824.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2725..4671
                     /locus_tag="PW101_002453"
     CDS             2725..4671
                     /locus_tag="PW101_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HDL2514825.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1513 a          813 c          779 g         1652 t
ORIGIN      
        1 atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac aactcctttt
       61 tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt atagacatta
      121 aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt aaaatataat
      181 ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata agacttgcaa
      241 tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca atgaaagagg
      301 taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt tgatgagcaa
      361 ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct ggtttgtgat
      421 ttataatagt tgactttatt tccatttcac gacggatagt agacagatta ttaggaattt
      481 tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat tctgtgggag
      541 aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt ttttgtaaag
      601 tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa acattaggag
      661 ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca gcaattccaa
      721 gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt tcaatgtatt
      781 ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat ccatgtttaa
      841 ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta atattaggaa
      901 aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa ttatttggtt
      961 tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct gataaatatc
     1021 ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat tcagcgacat
     1081 cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt aggttcgcct
     1141 gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt aaacactttt
     1201 tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt agcagttgtt
     1261 tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata ttgaatgcct
     1321 gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa gctaagtcca
     1381 tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg aaaagttagt
     1441 tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga actagcacgt
     1501 aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat tgaaagttct
     1561 tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc gatatcaggt
     1621 agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc ttgagtcgca
     1681 tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag ttttactttt
     1741 tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc ataatttagc
     1801 tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc tacaaatact
     1861 agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag gttgccaact
     1921 tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa gcctgatttt
     1981 tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa catttgtgga
     2041 tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc acctttcgta
     2101 atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc ccaattttca
     2161 tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata tgcagaaccg
     2221 gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc tgttacatga
     2281 aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg agcagctcca
     2341 aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa accattagga
     2401 tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg tgatcgtttt
     2461 acattatcct taattttatc catctcatct ttagtccaat cagaataagc acgatatcgt
     2521 atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa ctagtatggt
     2581 aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat atgacaaacg
     2641 tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc gttttcacgt
     2701 aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga cgtaatacaa
     2761 gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct tcgtctcgta
     2821 ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga tgtgaaaagc
     2881 acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt taatttcgtt
     2941 tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga tttaaaacaa
     3001 attgctagta aagataaaaa attcaacccc attatggcat taattaaatt attatcagat
     3061 atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc attacaaaac
     3121 tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta tcctgcgatt
     3181 gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt cttaccaatt
     3241 ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt
     3301 ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa tatttttggt
     3361 tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc agctgtttgg
     3421 atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat tgactttaca
     3481 ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt tattggccct
     3541 gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata taacactact
     3601 ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat gactggttta
     3661 catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa tggtatcgga
     3721 catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg tgctgcaaca
     3781 tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt
     3841 gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt aaaataccgc
     3901 tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc aggattactt
     3961 aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc aattaatgca
     4021 acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt
     4081 acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga aattgctgaa
     4141 caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac tgatgttgaa
     4201 tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg taaagtgatt
     4261 agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa agggattgcc
     4321 attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca actcacgttt
     4381 gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct aattcatatt
     4441 ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac tactaatcaa
     4501 aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga gaaagaaggt
     4561 cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga agtaaatata
     4621 gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata aatatatttc
     4681 cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat caaatatttg
     4741 agaataacat tttattt
//
LOCUS       DANKHL010000197         4714 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000197 DANKHL010000000
VERSION     DANKHL010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.207"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(673..1491)
                     /locus_tag="PW101_002454"
     CDS             complement(673..1491)
                     /locus_tag="PW101_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2514826.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1652..2341
                     /locus_tag="PW101_002455"
     CDS             1652..2341
                     /locus_tag="PW101_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL2514827.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2355..3857
                     /locus_tag="PW101_002456"
     CDS             2355..3857
                     /locus_tag="PW101_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2514828.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3870..4346
                     /locus_tag="PW101_002457"
     CDS             3870..4346
                     /locus_tag="PW101_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2514829.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(4443..4553)
                     /locus_tag="PW101_002458"
                     /pseudo
     CDS             complement(4443..4553)
                     /locus_tag="PW101_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1592 a          673 c          829 g         1620 t
ORIGIN      
        1 tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat gaactaataa
       61 ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc ataaactttt
      121 aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca taacattgaa
      181 gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt cttataaaca
      241 agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt tatagaaaac
      301 attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc agtaggttcc
      361 tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt atttaagaga
      421 ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta cacgtttttc
      481 tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc aagaaccgta
      541 ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa taagctatca
      601 agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta ggtttagcta
      661 agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt tttctcttaa
      721 ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat cacaaatata
      781 aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa tggctgtttg
      841 taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta tagcaataat
      901 ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg ttttcccaga
      961 taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt gggcttgaaa
     1021 atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat tttctaaatc
     1081 aagactagta tttccactag atcccactcc aaatatatat atgttttttg attgagtgat
     1141 cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg aatagagagc
     1201 ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat gttctttttt
     1261 ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag aaaaaccaat
     1321 tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct taatatcact
     1381 catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga cttttttttc
     1441 agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca tacaaactcc
     1501 tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact
     1561 ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag ttattatcca
     1621 aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt tagatcttat
     1681 ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt acaaagagga
     1741 agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag ttggtatacg
     1801 tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac caatgattgg
     1861 aattattaaa agagattatc caccagaatc tccattcatt actgccacta tgagagaagt
     1921 agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc ttagaaatcg
     1981 acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat atcctcatca
     2041 gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata aagcaggaat
     2101 agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac aagatgggcc
     2161 agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg ctgagggaag
     2221 gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag gtattgttgt
     2281 gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag cattaagtta
     2341 gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc atgttaccaa
     2401 ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta acaaatgaga
     2461 gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt ttaagtatta
     2521 tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca attggtattg
     2581 ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga gtagcttatc
     2641 tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa gatgcaacac
     2701 tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt ttacacaatc
     2761 gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca agatttattc
     2821 cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta atttggccac
     2881 caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt agtttaggaa
     2941 cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac cacaccattt
     3001 accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga tcaacaattg
     3061 ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact ggtctgttta
     3121 cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga ttaccagcag
     3181 catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat ggtgggcttt
     3241 attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt gaatatatgt
     3301 ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga ttatcttttt
     3361 attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt ttgatcgact
     3421 ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa ttaattcctt
     3481 ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc aaaaaattta
     3541 aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa gtaataaaca
     3601 aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt ggaaaagaaa
     3661 atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg aatggggaga
     3721 ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa gtgaagggtg
     3781 gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc aatcaaatat
     3841 taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag aaaaaacaag
     3901 aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta aatgatcctg
     3961 tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt tcaaaaattt
     4021 atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct attaatataa
     4081 aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt gaattacaag
     4141 gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa acctcgttgg
     4201 ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt atagttgttt
     4261 ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt gctcatggga
     4321 attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa aataagctaa
     4381 ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt ctgttcattt
     4441 ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt aaagttataa
     4501 aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt caaatataaa
     4561 atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg tttactcaac
     4621 ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga accttagcac
     4681 tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DANKHL010000198         4688 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000198 DANKHL010000000
VERSION     DANKHL010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.208"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(366..1157)
                     /gene="msrA"
                     /locus_tag="PW101_002459"
     CDS             complement(366..1157)
                     /gene="msrA"
                     /locus_tag="PW101_002459"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2514830.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
     gene            complement(1348..1983)
                     /locus_tag="PW101_002460"
     CDS             complement(1348..1983)
                     /locus_tag="PW101_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL2514831.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(2249..3301)
                     /locus_tag="PW101_002461"
     CDS             complement(2249..3301)
                     /locus_tag="PW101_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2514832.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(3314..4252)
                     /locus_tag="PW101_002462"
     CDS             complement(3314..4252)
                     /locus_tag="PW101_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL2514833.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT         1525 a          987 c          719 g         1457 t
ORIGIN      
        1 atagttattg tttgcgcttt tatacggaat ttagttcatc attaatgtgg aattcgatta
       61 ggtgtctatt aattatcaag atgattctct acaaaattcc atagaaaaat caagattcta
      121 caattcgaat tctctttcac atggaaataa aggctagtaa aaaaacagga gttgtgacaa
      181 aagacttgtc atacctcctg tttccttgcg acttgaagct gaatgctttg cgccacaacc
      241 ttttcctcgg tgttctcaaa gcaactcctc gaaataattc ataactgtcc aaaaatatga
      301 aaagctattt ttgtcagttg ctcattattt tgtcggagct gaacgctttg cgccacaacc
      361 ttcctctatc tatgtcgctg aaaaaataaa tgaagctgat gcgaatgctt gatcttttta
      421 tatcttttag gttgcttctg ataaaattgt tgatgatgat tttcagctgg ccaaaaagtc
      481 tttgcttggc gaatctctgt aacgatagga cggctaaatc ggttagattg aatcagttgt
      541 tctcttgatt tttcagccaa ttgcctttgc ttttcattaa caacaaatag aattgaacga
      601 tattgtttcc cccgatcttg aaattgacca aaggcatctg tagggtctga aatttgccaa
      661 taaatctcca acagctcttc ataggttatt tgacgtgtat caaatatgat ctcaacagct
      721 tcaacatgcc cagtatatcc accactgact tgatcgtaat ttggtttttt ggtctctcca
      781 cctgtataac cagataacac tgagattaca cctggttttg tttcaaaagg ctcaaccata
      841 caccagaaac agccaccggc aaaaatcgcc cgttcttggt ataagggtga agttatctta
      901 tgtttggcat aatcataagc ctttgcactc gctgactccc ctgtgattct taaataaaaa
      961 tccatcgcat ctggtgtcaa attatctcgt agtgctagcg gacgcaagtt gacctctagt
     1021 ttttctatca cctgctttat cgacattttt gctaggtctt cttttgcctc ttcaaattgt
     1081 gttctttccc attctcttgt atttcgattt aaaataatat tataaaggtc tatttccatc
     1141 tgctccacat ctaacatgaa gtcaccctct ttcaagtttt cttcttattc attcaaaaaa
     1201 tgactacctg aaatttagca caacgcataa caagttaaga ctaacttgct tttcgctctc
     1261 ctcatctcta ttcttagtcg aacacatcca cacgaatggt tacccataga gaaattcttt
     1321 tacttaatca gcttcacact gctttttcta ctttcgtagt tgaaaaaaac ttaaacaaat
     1381 catcggcaaa tagttaaaga aaaaacttaa gcgttgaaag aagcccaatt gcttaaagaa
     1441 ggcaaactga ctaatcgacg gaatccgtgc taatccgtaa agacctgcaa ttagtaaact
     1501 aataactgtt aaaacaagat aaaaatggct aagcgtacct tgaccacttt gacgataaag
     1561 cagaacaagt aacaatggga aaagtagaaa gcctgcatag cctagcccag aacctatatt
     1621 atggatccaa gtcgatagat tccaagagct ttcattcgta ttgatactga ataacccagt
     1681 aaaaatacaa tcacctatcc catataaacc aactgttgca acagcaagta ttgcaagtgt
     1741 ttttgatgtt ccaataaata ggtgatacaa cgcaggcaac gaaagaacaa aaaaaagtcc
     1801 agatacaact gaccagacca agaaagcacg tctgactgga ctatctacgt caccaaacac
     1861 actaattacg gtagtcattt gattcagttt gggataaaaa aggccaagga tatacggtgt
     1921 cgataaatca ctaatcacac ctaataataa aaaataaaaa ccatattttt gcaaaaaaat
     1981 cattttcttc tcctactttt tagtgattat tcgatacatc caacaatcaa attttttctc
     2041 attttaacac atatttttat tctagttgcc ttttgaacaa tcaataacat aaaggtgagt
     2101 ttcttctaca ttttaaaaaa tcgaatggca ctgaagagtt cagttgtcag catcaatctc
     2161 ctactcaaaa aggcaaaaaa acgtaagtca gatgttgttg acatctcact tacgcatcca
     2221 ttgattgaaa atttcttagt aaaatctact agcctaaaaa tttgaagact tcttctcgat
     2281 catacagttt aaatactttc tcgcataaaa attgagtaat tagtgccgca aaaacaggga
     2341 tgactaacca acaaagaaca acaagaaagc catttagccc tgcatctaaa gaagccaacg
     2401 gaccaacaag tccgaccacg ccgaaaccag cagaagcagg cgtacctgaa atagaaaata
     2461 gagcgacagg aatagctgta atcactgcac taactaaaca aggaataaga atcacaggtt
     2521 ttctaaatag attaggcatc atcatcttca tagcgcctaa agcaatcgca attgtcacac
     2581 ctgacttatt aaccttccaa gagttcacta ctaaaacaaa agtggttgca gctacgccca
     2641 tcgcagccgc accagcagaa acaccatcta gttggattgc cattccgatt gctactgttg
     2701 aaataggaga gataatcaaa aaggcaaagg aacaaccaat caagatagac attaacagcg
     2761 gttgtaaggt agtgaagtta ttaatcaatg atccaattgc agaagtaatt gctgttacaa
     2821 aagggaataa ataaaaacca agtaaacctg cacctacacc tacaacaatc ggtgttaaca
     2881 caatcgacac tgacccaaaa ctatttccaa tcagtaaaat cagcccgaca gcaatcgatg
     2941 ctgtcagcat cgtattaata atatcgcctg taccagcacc aacgtaaaca cccgtttcac
     3001 ccaaatcagg tgtgaatttg actacaccag aaccaacaaa agaagctccg gctacaacca
     3061 tcattctcat cggatcaaat ccaaattgta aggcaatcaa accaccaatg atcaaaggtg
     3121 ttgctagttg aaaaataact gctaactgtg cgatcgttac cgcaaaggga tacgcactaa
     3181 aatattttaa aatggcacct aatactgcat taggaatcaa tccaataatg attcctgttg
     3241 ctgtaccagc gaggacttta ttcagaaaaa ttttcggtga tattttttgt gaagtttcca
     3301 ttcaatctct cctttatttt acgttcaaac tatcttcttt tgcatggaca agctcagtca
     3361 agaaatcaca ataaggcgtc gcaacaccat atttcttccc ttttctagaa attgcgccat
     3421 tgatataatc gatttctgtt aatcgatggt tcttaatcaa gtcttgatac attgaaggat
     3481 aatgtaaccc aattgtttcc gggtcaaaac aagattcaca atgtgcaatg acttctggga
     3541 catctagttc aatcttctct actgctgcta ctttggcaaa ctcattgaca atcgtcgcca
     3601 ccattttatg agcagtcgat gtttttccta gctcagccat attaacgtct aagattgtgc
     3661 atagtccgtt cattgttccg ttcacacatg ctttgcgata aatagaatag tgaatattgt
     3721 cagaaaaatg agcatttaat cctgattcag acaatttgtc tgctaatttt tttgcagctg
     3781 cttcttttcc atcacctaaa ttttgtaatt ctactgaacc acttccaaat aatttgacct
     3841 gaccaggacc ttcaagacct gccgtccaca tggtatttcc aatatagata ttttccattg
     3901 gtacaaattt ctcaataata tcttcatgcc cgataccatt taatagacat aagacctctg
     3961 tatctttttt gattaatgat tggatatcct gcagcatttt ttccagctgc atcgctttgg
     4021 taaatagaat aatcagatca acttgatctt ctttttctac ttcggattga agaacgattg
     4081 gtagttttgc ttctacctct ttaccattaa aatttgcttg taatccatgc tctttgattt
     4141 gttggacatg ttctgcccag ccatcgatta ataatacttc gttcccactt tggtgaagca
     4201 ttaacccaaa gcgacttccc attgcgccag cgcctgcaat tgctattttc attgattctt
     4261 cctccttttt ctttctatta tattcatttg agaatcattt cacaaccttg ttttgttatt
     4321 cttctcttat ttatcatgtt tatatccacc attcaagtta ttttatcagc aaatcaataa
     4381 aaactggaat agaatcccaa tttcatcgta agagaaacta gactagcaat aagaaaatag
     4441 aaacattcat agaaaaaacc tcactcctct gattataaaa gagggggcat taacctcgtg
     4501 tatactaaaa ccttccattc attcgatgga tgattttctt tttgtgaaaa aatattcatt
     4561 gtagtacgat gttgcgcaac acagacggat ggtattgctt attatttatt aaaatcgaac
     4621 aatacactta tttaaaaact tctttcccag attttcttcc atttttacag ttataactag
     4681 tattatca
//
LOCUS       DANKHL010000199         4657 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000199 DANKHL010000000
VERSION     DANKHL010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.209"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(459..884)
                     /locus_tag="PW101_002463"
     CDS             complement(459..884)
                     /locus_tag="PW101_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514834.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1258..2229
                     /locus_tag="PW101_002464"
     CDS             1258..2229
                     /locus_tag="PW101_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2514835.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2261..3658
                     /locus_tag="PW101_002465"
     CDS             2261..3658
                     /locus_tag="PW101_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2514836.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            3817..4440
                     /locus_tag="PW101_002466"
     CDS             3817..4440
                     /locus_tag="PW101_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514837.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         1616 a          754 c          950 g         1337 t
ORIGIN      
        1 aattactacg tgatgtagaa taagcctttc tatcgtttgg ttattttata ttcaaatatt
       61 tgaaataaat aaatctttac tctttgattt gtaaactgaa atgatccact gtttgtttta
      121 ctgatgaagc ttgatcaaag gaagaagctg agtttgaagt tcataaaaat cagccatagt
      181 gagttttgct tggatttgtt tatcttcttc tactttttac ataattttga acaggataag
      241 attcaaatgt tttaaatgaa tctttgacta gtttgtgaca gttgtcgcat atgctcaagc
      301 ttccttggtc attaaattct gtatggatat ttttgttgtt attacatgta caataatcag
      361 gactatcgat catccatttc gtctccttta caatcatctt gcttctttac tatagcagaa
      421 aagaagggga gaaacaaaag aagatccaac agacggcact atagactgta gatgttgcca
      481 tcctcatcct tcaaacggta attaccgatt tttgtaaata tgactgttgt ttctttttta
      541 ttttcttttc ctgaaataat atattcgtca tctttggtag tgatcttgat atctgtcagc
      601 actgtttttt cttcagatga ttcagaagaa tcagttcctt gtgtctgata cgtgcgtgtc
      661 cattccattt ttttatctgt aaatgtcaac tgtcccgcgg ataaatccat atctttacgt
      721 acttttagcg tatatacact gttggtggta tctgcttttt ccgcttttaa gacagaagaa
      781 tcggttgttt cgtccgagga ttggtcagag ccgccaaaca aatcacagcc agtcaatgtc
      841 aaaagtcccg cagacaaaat aaatagtacg attaatttct tcaaaatact tcccccattt
      901 gataggataa agaaattata ccattggaat gtaaaacttc aaacggatgg aagagaaaaa
      961 cgcacaaaac acccacaaat agagtaaatt ttagaataaa attttaaaaa ggcaaaaaat
     1021 ccgccacttt cagaaagcgt tttaaaaaag tgttgcaatc ttttaaaaaa atgctatatt
     1081 aaaaatgaac cggttcaaat acttgtttaa gtataattaa tagtgagagg aagttgctct
     1141 actgaagact ttagttatat ggatattgat gacaagcaac actatgcaaa gaatgcggtc
     1201 atcataatcg aacaacaaga aagagaaatc aaatgtaact ggcaggagtg agaatgtatg
     1261 gcatcgatcc gtgatgtggc taagatggct ggtgtttcag tgggaacagt ttcccgttac
     1321 ttgaatggtc agcaactgaa ggaaaagaac atgaaaaaaa tagcagaagc gatttcagcg
     1381 ttggactata aagaaaacat cattgctaaa gggttgaaga ataatcgaag cttctctatt
     1441 ggacttttga taaacgggat ttccagccgt tttgggtcgg aagttgtctc agggatcgaa
     1501 agagtagctg aagaaaatgg gtatagctta ttattaagcg ggtttgccga tcagccagaa
     1561 aaaattgaac aaaaaattga atacctgatg aagcatgtga tcgatgggct gattgtcttc
     1621 ttatccgaag aagagtggtc tggatttgaa atgctcacaa aaatgagtat tccagttatc
     1681 gcattgaatt gtccagaagg accagcaggt attgatacaa tattagtgaa tgatcgagaa
     1741 agtgtagcaa aagtgatcgc tcatcatggt gaacaaggac ataaaaaaat cggatttatt
     1801 gctgccactc aaacagacta cgtagcaaga gaacgtttag caggagccaa agaagcggtc
     1861 caaaatgatc catcgatcca attagaggtc tttacaggag actattctag aaaaagcggc
     1921 tattacgggg caaaagcatt attagaaaaa ggggcaactg ctatttttgt cagcaattac
     1981 aatatgtcga ttggtgccat cgaatgtttc aatcaagaag gtgtcagaat cgggaaggat
     2041 attgcgttca gtcactatga ttatttagat aaaaacgatc tgagtatcct tccgaaaatc
     2101 acgatcacgc caccaacaga taaaatcggg gaacgagcag cagaacagct gttaaaacga
     2161 atcaaaaaag aagaggaaga aaatgaaaaa gtgattgtga tggaaaatat catacatgga
     2221 atcaactaaa acgaactaaa aaagaaaaag aggtgctttg atgaaaaaat atcaatttcc
     2281 agcaaatttt tggtggggtt ctgcagcaag tggcccacaa acagaaggaa ggatttcagg
     2341 cgatggaaaa ggagagaaca tttttgatta ttggtacaaa aaagaaccgc aaaagttctt
     2401 tgatcaagta ggacctgaaa aaacctcgca agtttatacg aagtatcaag aggatgtaca
     2461 gttgatgaaa cagactgggc ataattcatt tcgaacatct atccaatgga gccgtttgat
     2521 acctgatgaa tctggaacaa tcaacgaaga agccgttcgt ttctatcatc gttattttga
     2581 tgagttgatt gaaaacggta tcgaaccctt tgtgaatctg tatcatttcg atatgccgat
     2641 gtatctgcaa gaaaaaggcg gatggttgaa ccgagaaacg gttgaagcct atgaaaaata
     2701 tgccaaaagt tgttttgaat tgtttggttc gaaagtaaaa aaatggttta ctcataatga
     2761 accaatcgtt ccagtcgaag caggttattt atatcgatgg cattatccag aagagtcaga
     2821 tatgaaaaaa gcgatccaag ttggctatca tgaagcgttg gcttcagcgt tagccatcaa
     2881 atcttaccat gaaatggagc aaggtggtgt tattggtatc atcttgaatc tgacaccaag
     2941 ctatccaaga gacgaatcaa accctgaaga tgttaaagca gcgcaaatag cagatgcgtt
     3001 cttcaatcgt tcattccttg atccagctgt aaaaggcgag tttccaagtg aattgattga
     3061 gatagtaaaa gaacttgatc tcatgcctgt gatccatgca gaggatttga cggttatcaa
     3121 agaaaataca gtagatcttt taggtatcaa ttattaccag ccacgccggg taaaagcaaa
     3181 agaaacacca atcgatacta aaaacggtcc aatgcctgag gattatttcg acaattatga
     3241 aatgcctggt cgtaaaatga atccttatcg cggatgggag atctatgaac aaggaatcta
     3301 tgatattttg atcaatgtac gagatcatta tggaaatatc gactgcttta tttctgaaaa
     3361 cggtatgggt gtagaaaacg aagcacgatt tatcaaagaa gatggcatga tccacgatga
     3421 ttatcggatc gaatttgtcc aatctcatct gaaatatgtc catcaagcca ttcaagaagg
     3481 tgccaattgt ttaggctatc atatgtggac atgtatggat aactggtcgt ggaccaatgc
     3541 atataagaat cgatatggct ttatttcagt agacttagca caagatggaa aaagaacaat
     3601 caaaaaatcg ggtcattggt tcaaagacgt atcagataaa aacggatttg aggcttaaga
     3661 aagaatagtt gagagcaaat gcgtgaaatt ctccattctt accattttgt gaaaaagaag
     3721 ttcaattatt tacgatcttt ttcaactttt ttgtctttct atttcatttt ttgtatataa
     3781 tagaatagtc aaaattaaat aggagtggag gaaagcatga ggaaaaaaag accgtttctt
     3841 ctcttttggt tattcggttt tttcttactg cttattttat ccgcagcatt ttcaggaaaa
     3901 atgacagcag atcttgctca gaaagaaaca gaaaaaatcg tagcaagcaa agaatcagta
     3961 gataatttca aaaaagaatt ctcaacttat tggtgggcgg agtataaaat aactgccaat
     4021 tacgaaaaag gcgaattgga catttatttg ccaaaaggaa aagaagaaaa tcaatttatt
     4081 aatatcgatg acgtacttag tgcgatttct ctcagacaat atcaatttaa agacaagcat
     4141 ttgaccgttt tgcttttgac tcatgatggc gacaaagtag cccgtttgaa aaatggaaaa
     4201 gtgatcaccg cttattctga aaaagtagac aaaaaagtgg tcgccgatcg tttagcggaa
     4261 tggaaaaaga caaatgaaca agtcaagaaa acaactgtct cttctcaaaa aaacgagtcg
     4321 acaggaacaa gtccttcaga ttctggcagt ggacaaacga taacgggaag tgaagaaaat
     4381 caagcctcaa ttattacgaa tgagtcatca caaggacaat atcaatccta tggacaatga
     4441 aaaacagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa acggcaaaat
     4501 agttcctcat attttgccgt tttttgagct aataccgaag gtcagctgat tgaacaccgt
     4561 gtatcaagag gtcttgagcc tgccgtttaa ctctgagcca caaggagcaa tttcaaaaaa
     4621 tagctcctta tatttttcgt ttggttgggc ttattac
//
LOCUS       DANKHL010000200         4627 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000200 DANKHL010000000
VERSION     DANKHL010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.210"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            11..757
                     /locus_tag="PW101_002467"
     CDS             11..757
                     /locus_tag="PW101_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL2514838.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            754..1593
                     /locus_tag="PW101_002468"
     CDS             754..1593
                     /locus_tag="PW101_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL2514839.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            1594..2544
                     /locus_tag="PW101_002469"
     CDS             1594..2544
                     /locus_tag="PW101_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL2514840.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            2685..3437
                     /locus_tag="PW101_002470"
     CDS             2685..3437
                     /locus_tag="PW101_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514841.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            3456..4607
                     /gene="queG"
                     /locus_tag="PW101_002471"
     CDS             3456..4607
                     /gene="queG"
                     /locus_tag="PW101_002471"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDL2514842.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         1566 a          774 c         1008 g         1279 t
ORIGIN      
        1 agtgtaagat ttggaacgta cggccattgt aattcaagac ctttcagccg cttatcaagg
       61 gaaaactgtt ttgaaaacta tcaacttgac gataaatcca caaaaaatta ccggtattat
      121 cggaccaaac ggtgcaggaa aatcaacatt tatgaaatct ttgctagagt tgataccagc
      181 tactactggt aaagtcacct tttctggaca gtcggtaaac tcagtccgta aaaaaatagc
      241 ctacgtggaa cagcgaagtg aattggatct ttcctttcca attgatgtat taggcgttgt
      301 acttttagga acatatccat ctttacgaat tggacaaaga cctgggaaaa aagaaaaaga
      361 acgtgcaaga caagctttga aaaaagtagg gatggaagaa tatgccaaaa gacagatcag
      421 cgaactatcg ggtggacagc ttcagagagt ttttattgca agagctctag cccaaggagc
      481 agaatggatc tttttagatg aaccattcgt agggattgat gcgttaagtg aacgaaagat
      541 ctttgacatc ttgcaggaat tgaagaattc aggaaaaacg attttgatcg tccatcatga
      601 tcttcataaa gtagaagaat atttcgatga ggttattctt ttaaataaac agctgatcgc
      661 ttccggtcca gtacaagaag tctttacatc gaagaacctt caattggcct atggtgagat
      721 catcagacat ttagtaaaag gaggagagaa aaaatgatcc aatcctttat tgatggatta
      781 atgaattatc aattcctgca atacgctttg gtcacttcca tgcttgtagg acttgcttct
      841 ggagtcatcg gttcttttat tattttacga gggatgtctt tgatgggaga cgctatctct
      901 cacgcagttc ttccaggtgt agccatttct tatatgtttg gttttagtta catctttgga
      961 gccactgctt ttgggatgct aacagcagca gtgattggtt tcgtgactca gcggagccga
     1021 ttgaaaaatg atacagcgat tggaattgtc tttagctcct tttttgcatt agggattatc
     1081 ttgatttcat atgcccagag tgcgacagat ttgtatcata ttttatttgg gaatgtctta
     1141 gctgttaggg aaagcgattt gttgctgaca gcactcgtta gtggaatcgt cttgatattt
     1201 gtctttttct tttataaaga attgaagatc acatcattcg atccgacaat ggcaaaagct
     1261 tatgggctga acacatcgct gatccattat cttttgatgt tctttttgac attagtggct
     1321 gtagtcagtt tacagacagt aggaacaatc ttggtgattg ccatgttgat cacaccagcc
     1381 gccacggctt acttgctaac gaaccattta ctgaaaatga tcattacagc tgcaggaatc
     1441 ggtatgctaa gtgcagttgt cggtgtgttt ttcagttata gttacaattg gccatcagga
     1501 gctacgatcg tgttagcatg taccgcattt tttatccttg cttttttatt ttctccaaca
     1561 aaaggaattt tatttcagaa aggaagcaga tagatgaaaa caaaaaaatc actcttttta
     1621 atcttggcag ttagtttctt ggtattagcc ggttgcggaa agcaggcttc tgaccaagcg
     1681 gatgaaggaa gtaaagaaaa gttgtcagtc gtggctacca attcgatctt ggcggacatg
     1741 gcaaaagagg tcggaacaga tcaaatagat atccacagta tcgtaccagt cggaacagat
     1801 ccgcatgaat atgaagtact accagaagac atcaaaaagg caagcgatgc agatgttatt
     1861 ttatacaacg gtttgaatct tgaaacaggt aacagctggt tcgataactt gatggaaacg
     1921 gctaaaaaag aagaaggaaa agattatttt gcagttagca aaaatgtaga acctctttat
     1981 ttaactagcg gtgaagaaca tacaaaagca gatccccatg cttggctaga cctatctaac
     2041 ggaataaaat atgtggagga aatcgcacgt atattctctg aaaaagatgc agaaaatgcg
     2101 acactctata aaaaaaatgc agaagcatat gtggaaaaac taaaagaatt agatacacaa
     2161 gcgaaggaaa gttttgcttc tatcgaagag aacaaaaaat tattagtaac aagtgaaggg
     2221 gcattcaagt atttttcacg agcatatgat ttgccagcag cttatatatg ggagatcaat
     2281 acagaaagtc aaggtacgcc tgatcaaatg aaagcgatca ttgatcagat aagagcaaaa
     2341 gaagtaccgg ttttattcgt ggaaaccagt gtagactcaa gaagcatgga acgggtagca
     2401 aaagaaacgg gtttgaaaat ctatgataaa ctgttcactg attccatagc aaaagaagga
     2461 gaacaaggag attcttacta tcagatgatg aaatggaata ttgaaacgat ccatgaaggt
     2521 ttgtcacaaa caaaagaaag ctgaaaagtg caaggtctag cttgaggagc agaccttgca
     2581 ttttttataa gagatatgaa aaaaaattat gctgtttttc gtcgtacaga tacctttttt
     2641 gaagaaattc cgttatagtt agagtaagga aggaaggtgt ggctatgaat tttacacgta
     2701 aagataaata tacgattaaa gaagaagtct ttcgattgat cagccagcag gaaaataccg
     2761 acaaggatca agaccttcaa tttcttgaag cagaattagc taaattattc atagcaaatc
     2821 aaaatgagac aacagaacca gatgaagagc tgtacatagg caaatggttg tttgatgaat
     2881 ttcagccttt agatgaaaat accggtatcg gttattttgt gttcctttct ccagtcggct
     2941 tcacacctgg cgtattagtt aaacaaaacg acgaaataac cgtcaaagca tggcctgcat
     3001 tattttcagt catcaaagca gaaggagtaa tcaaaaagat ccagtcggcc tattataaaa
     3061 gtcaatttat tgccaacagc aaattaatga atatgtttta taatcgtcta gtagaaattg
     3121 ctaggcctgg gaaaatcccg caaacattca cgtacacaca gcaaaaaaat ctctatgaag
     3181 aagtattgta ttcccgcgat ctgtctgagt tttcgccatg ggaagaggta aggctccatg
     3241 aactgttaag tacaaaacca gtttcattga atttttttaa tgcattagat aacaaaagaa
     3301 aattaatttc aacaaattat tgggaagtat ttgctgcaag aaaaaaatgg atggaagaaa
     3361 agaagattgc cgatcagcag atccagtttg tccagctgaa aaaagaaaaa ctatattatg
     3421 ggttatacag ccgttaacag caagcgagga agaccatgac attaaaagaa gaaatcatac
     3481 aagaaagcaa acgattagga attgacaaaa tcgggttcac cacggcggaa ccttttgatt
     3541 cattaaaaga atcgttggaa gaacaaaaag cagctggcca tacttctggc tttgaacatc
     3601 caaatatcga tgagcgtctt tatccagaaa tgacatttga tcagccgaaa tcgatcattt
     3661 ccattgcact tgcgtatcct actaggatcc atgaagaagt gcctagggat gaaaaacgcg
     3721 ggcagtttgc tcgtgcttct tgggggatcg attaccatga tatactcaga gaccgtttga
     3781 accgattgat cgaattcatc caaacaagag cagaaaaatg gcaaaaagaa gaggaatggc
     3841 gtctagctcc gcaagtagat actggagaac tgatcgatgt cgctgtagca cagcgagctg
     3901 gtttaggatt tattggacga aacggattgt tgatcactga agagttcggt tcatttgtct
     3961 atcttggtga gatcgttact aatattgtat ttgagcctga taaaccagga gaatttggtt
     4021 gcggtgactg tatgcgctgc gtgactgctt gtccgacaaa agcattgctt ggtgacggaa
     4081 gaatgaatgc ccagcgatgt ttgtcttatc aaactcagac aaaagggatg atgccggaag
     4141 aatatcgtaa aaaaatgcga aatgtcattt atggatgtga tatctgccag ttagtctgcc
     4201 cttacaatca aggaaaagat tttcattttc atgaagagat ggaaccaaaa gtagacgaaa
     4261 tatatccaaa attaaagcca atgctttcga tgtctaataa agacttcaaa cagcaatttg
     4321 ggcatttagc tggttcatgg cgtgggaaga aacctttgca gagaaacgca ttgattgctt
     4381 tagctaatct aggtgatcga acggcattgc ctgagatttc agaatgttta acagatgtcc
     4441 gccctgtcat cagaggaaca gcagcttggg ctattggacg tctaggaaca aaagaaccgg
     4501 aaaaatggct tgatgtatta gatgaaatat tagctaaaga aacagaagaa gtcgttataa
     4561 acgaattgga acatgcccag aaattattgc ggagaaagtt aaaataaaaa aatagttaac
     4621 attattc
//
LOCUS       DANKHL010000201         4600 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000201 DANKHL010000000
VERSION     DANKHL010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.211"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            33..1094
                     /locus_tag="PW101_002472"
     CDS             33..1094
                     /locus_tag="PW101_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL2514843.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1097..1993
                     /gene="murQ"
                     /locus_tag="PW101_002473"
     CDS             1097..1993
                     /gene="murQ"
                     /locus_tag="PW101_002473"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL2514844.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2007..3989
                     /locus_tag="PW101_002474"
     CDS             2007..3989
                     /locus_tag="PW101_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2514845.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4054..4500)
                     /gene="nrdI"
                     /locus_tag="PW101_002475"
     CDS             complement(4054..4500)
                     /gene="nrdI"
                     /locus_tag="PW101_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDL2514846.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         1488 a          818 c         1043 g         1251 t
ORIGIN      
        1 aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata tttatgaacg
       61 attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat ggctttgtcg
      121 gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa aagcgattga
      181 tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat atttcaggag
      241 aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa gcaattggtg
      301 taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac tggtcacatc
      361 aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat gaattgaaag
      421 aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca cgtcccgaaa
      481 cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt caaggtctcc
      541 ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat caaggattgc
      601 caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat ctattagcaa
      661 gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt caggagcaat
      721 ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta gatgaagaat
      781 tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga gatgtcatac
      841 gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat acagctacgc
      901 gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg ggtgagatcc
      961 aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct aaagttatca
     1021 aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa tttataagaa
     1081 aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg aacgcagaaa
     1141 ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac taatgaacaa
     1201 agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg aaccagtgat
     1261 tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg gagccggaac
     1321 aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg gtgtagaacc
     1381 ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag ctgtcgaagg
     1441 ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt tgacaaaaaa
     1501 tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg gtggattaga
     1561 ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac aagccgaaat
     1621 cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt tgacaggctc
     1681 tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt ctactatcag
     1741 tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac caacaaatga
     1801 aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg attacgaaac
     1861 agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg tcatgatttt
     1921 gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg gatttgtaaa
     1981 agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg gaagaactgg
     2041 caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt gtccactgca
     2101 tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct gaattaaaag
     2161 cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc atcggacctg
     2221 ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa ttaggagaaa
     2281 aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa gaccgtgtaa
     2341 acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca tctaaaatca
     2401 aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca tttgtcggta
     2461 ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc ggaacacttg
     2521 atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac gggatgttta
     2581 gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca acaccaactc
     2641 ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa gcaccactga
     2701 aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta ggtgtcatct
     2761 tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta cctgatgcgg
     2821 tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt gaaattttct
     2881 tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc aactgggtac
     2941 ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg ccgatggtta
     3001 tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat gaaactggtc
     3061 gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt gcagcattgg
     3121 cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa ggagctttac
     3181 cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta cctctaggac
     3241 gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc ggaatgatcg
     3301 gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca ttgatctctg
     3361 acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt ggcttcgttg
     3421 ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta gcgactgaaa
     3481 cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct gaaagtgtga
     3541 ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca agtgatccag
     3601 tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac ggacatatct
     3661 attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct attgggatca
     3721 caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt gacttaggcg
     3781 gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat acgcttgtag
     3841 cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg atcgtcttgg
     3901 taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa gcaaaagcaa
     3961 aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac gaaagaagaa
     4021 actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg ctaaaatctg
     4081 ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca agaaaggaaa
     4141 gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga agtttttatt
     4201 tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca gatattcacg
     4261 catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc cgtctaaata
     4321 agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat tttcatggat
     4381 ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt cagcatattc
     4441 tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata aaatgttcat
     4501 ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt ttggtggtgt
     4561 aatttggaaa gggactaggc gctttgcttg tataatgaga
//
LOCUS       DANKHL010000202         4566 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000202 DANKHL010000000
VERSION     DANKHL010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.212"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(27..1259)
                     /locus_tag="PW101_002476"
     CDS             complement(27..1259)
                     /locus_tag="PW101_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDL2514847.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1514..2083
                     /locus_tag="PW101_002477"
     CDS             1514..2083
                     /locus_tag="PW101_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2514848.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2261..2701)
                     /locus_tag="PW101_002478"
     CDS             complement(2261..2701)
                     /locus_tag="PW101_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDL2514849.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2859..3623
                     /gene="map"
                     /locus_tag="PW101_002479"
     CDS             2859..3623
                     /gene="map"
                     /locus_tag="PW101_002479"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL2514850.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3655..>4566
                     /locus_tag="PW101_002480"
     CDS             3655..>4566
                     /locus_tag="PW101_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDL2514851.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSH"
BASE COUNT         1371 a          854 c          906 g         1435 t
ORIGIN      
        1 ttttttgcat gtgtgcgaaa gtatactcaa gcccaatcgc catttcggaa aatcggtact
       61 gtagagccat cttcacggat tccgtcgata tccatcttgt cagatcctac catgaaatct
      121 acgtgggttt gacttctatt caatccagct tctgctaatt cttcatcgga catttctgta
      181 ccgcctttca cactgaaggc ataagcagaa cctagtgcta agtgattcga tgcgttttca
      241 tcaaataatg tattgaaaaa gatgatgcca gattgagaaa taggtgaagg gtcaggtaca
      301 agtgcgactt ctcctaaacg acgcgcacct tcatctgtat caagcagttt tgctaagaca
      361 tcttctcctt gttcggcaga aaaatcaaca acttttccat ctttaaaagt aaatttcata
      421 cccgagatga tggtgccagc ataactcagt ggctttgtac ttgaaatgta accatctact
      481 cgatgactgt caggtgctgt gaacacttct tccgtcggca tatttgccat gaatttttct
      541 ccacgagcat tatagcttcc agcgccttcc caaagatggt ttttaggcaa tccgataatg
      601 atatctgtac cgggagcagt atagtgaagg gcagaaaatt gttctcgatt caattcttct
      661 gctttttttg ctaatttttc atcatgcttc ttccatgcta agacagggtc ttcttcatag
      721 acacgagtcg ttttgaaaat ctgatcccat aatgcctcaa cttgtttttc ttctggcagt
      781 tctgggaaga cttttgctgc ccattgttta ccagccgcag ccacgacagt ccaactgact
      841 ttatttgctt gagttgcctt gcgtaaattc attagtgctt tgccattcgc tgattgataa
      901 gacgctactc gatggctatc tacaccagca aatgcatcgg gatcggcaga aacaacgctg
      961 atcctgcttg ctcctttttc aagccattca tccgcttgat cgattttaga ttgtggtaca
     1021 ttctcaatac gatccgttgc agcatgtaaa agaaattctc tttggatctg gtcgtcagtc
     1081 cattgaacga taacctcagc ggctcctagt ttataagctt cttgtgtgat caatcgagca
     1141 aggggtgcct gttccacgct gatctgtaaa acaactgtgt gtcctttttc tgtggctacg
     1201 ccagtttctg caattaagcg tgcatatttt tttagaagtt cattaaaatc agataacata
     1261 ataaaaattc ctcctcacca ttttttctgg taactattag catgatacca tttaattatg
     1321 attcaaacaa tgaggcttta aataaaaaaa ggaggttatt tttttaaggt taagtttcat
     1381 gatatatgtt actacttgct atttcagctt gtgaagaaaa tcaaaaacta attttgcata
     1441 tcaaaaaaaa catgttacaa tgaaattaat caatggtgga agcgttataa aaaaattaag
     1501 gagattaaaa aatatggcaa gaaaaaagac gattactaga gaacagattt tggcagctgc
     1561 ttatgaagta gctgccaccg atggatttac acgatttact gcgagaaaca tcgcgaacaa
     1621 aatgaattgt tccacgcaac cgatttatct tgagtttaaa aatatggatg acttgagaca
     1681 agctcttttt gagcagatct atgatcattt acaatatgaa gtgttcactg ttgagcatac
     1741 agggaatacg attatcgatc ttgcttttaa ttatatccat tttgcaagaa gagagaagaa
     1801 gttgtatcgt tcactgtact tagaagaata tggtggagga aaagagatgc agaatttctc
     1861 ttatacttac tttgtgaata cagtgaaacg cgatccagct tatgtgaatc tttcagaaga
     1921 gcagatcgaa tctttgtata atgggacatg gatcatagct actgggatcg cagctttgat
     1981 gtcttcaagc atcatccatc caactgatga acagattgaa aagatgatcc aagacagtat
     2041 agatgctatc ttgaaattca aagaaccaat cgaaatcgat tgaatatact caaaagttcc
     2101 acaaatcagt aggtaacaag agatgtgtca aaataaggaa aagttcgttt ttcttacagc
     2161 gtatgcattt ttaactcccg aagaactctt ccatgaatgc aatgaataaa agagccatac
     2221 agattgaata aatctggatg gctcttttat tgcaagtata ttaaattttt gcgactagtt
     2281 ctttagcgaa agcttctaaa ttttcgatat cttcttcttc agcattcaga tccactttaa
     2341 cattttcggc tccttttgat gcgcctgttt ttgcaaaagc agcatcgaaa tcatccaccg
     2401 atttgcagaa ttcatcgtaa aaagtatcac cagatccgca gacaccgtaa atttttccca
     2461 atagatccaa ttcttgcaag tcttcataaa agtctacgat ctcatcaggc agatcgccat
     2521 catcgtatgt ataagtagct actacgcaaa tatctgcctc ctcgaattca tctgcatcta
     2581 cttgggtaca ttcgttgatt tcgacttcga tatccagatt ttcaaatgct tctgcaacaa
     2641 tatcggcgat ttcttcagtg tttcctgtca tgctagcata tacgattttt gctaaagcca
     2701 tgatgtgcct cctcgtttat aattatttaa caattatgaa ttataccaaa acatcattga
     2761 aaagaaaata aaacctagag acacctgggg aatgtcattt ggctaaatgt atgccgctat
     2821 gttaaaatga gagggatttg taaaaaggag acgaaaatat gattacttta aaatcagcac
     2881 gtgaaattga aatgatggca gaatctggag ctttattagc agatgtacat aaaaatctgc
     2941 gtgattttat aaaaccagga atcacaagtt gggatattga ggtatttgtc agaaactata
     3001 tcgagagtca tggcggaatt gctgcgcaaa ttggctttga agggtatgaa tatgcgactt
     3061 gtatcagtat aaatgatgaa atttgtcatg gattccctcg taaaaagcct ttgaaaaacg
     3121 gtgatttggt aaaagtcgat atgtgtatcg atttgaaagg cggcatttct gattcttgtt
     3181 gggcctatgt agttggagaa tctacacctg agatcgaccg attgatggaa gtaacgaaga
     3241 aagctttgta catcggtatt gagcaagcac aagtagggaa tcggattggt gacatcggtc
     3301 atgctatcca aacttatgta gaaggcgaaa atctagcgat cgttcgtgat tttattggtc
     3361 atggcgttgg tcctacgatt catgaagaac cagcagttcc tcattacgga gaagctggaa
     3421 aagggttgcg gttgaaagaa ggcatggtta tcacgatcga acctatggtt aataccggaa
     3481 cttggaaaat gaaaatggat cctaacggct ggactgcgta tacaagagac ggcggacttt
     3541 cttgccaata tgaacataca ttggctatta ctaaagatgg tccaagaatt ttgacatcac
     3601 aaggagaaga aggcacttat tgatctatcc agtcaatagg tacgggagag gtacatgaaa
     3661 gtttggaaca aaataaaaca aaatgaaaat ctgatgcgct ttattgagac gactcaaaag
     3721 cgcatcactg attcagaaat gagtacgacc tctgtcgtcg ttgcttatta tttactcttg
     3781 tctttatttc cattgattat tgcttttggt aatattttac cgtttctcca catcgatcaa
     3841 gagactgtac tgacctattt acgagaagtg attcctgaga ccgtttacca gtttatcggt
     3901 cctgcaatca aagacctttt gacacaaagt tcaggaggct tgttatccgt ttctgcatta
     3961 gcggctttat ggtctgcaag tcaaagtatc aatgctttgc aaattgcgat gaataaagcg
     4021 tatggcgtag aaaatagaaa aaattttttg atcgtccgtt tcttctcact aattgtcatt
     4081 gtcttgttca tgattgctat tagtggggta actatcgttc ttgggttggg acagctgatt
     4141 ttggatgcta ttcagccaat cgtacaaata cccgtgaact ttatcgatca gttccaaact
     4201 ttgaagtggc caatcacatt ggtagcttta tttgtcatca tgttcttgat ttatttaatt
     4261 gtccctaatg ctcagttgaa gttgaagacg atcattccgg gcacgatctt cgctactgtc
     4321 ggctggatgc tgttatcaca agtgtttagt atctatgcta ggtattttgc aacgaggatc
     4381 agcggttatc agatcatagg aagttttatt gtactcatgc tttggcttaa ttttgctgct
     4441 acgatcatca ttttgggcgg aatcatcaat gcagttgttc aagaatacgt gacgggtcat
     4501 caaatcaaaa aacgtcgaga tccaagccga aaatggttca ataaattgaa aactaaattt
     4561 tctcat
//
LOCUS       DANKHL010000203         4543 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000203 DANKHL010000000
VERSION     DANKHL010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.213"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            89..1093
                     /locus_tag="PW101_002481"
     CDS             89..1093
                     /locus_tag="PW101_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2514852.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1094..2038
                     /locus_tag="PW101_002482"
     CDS             1094..2038
                     /locus_tag="PW101_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2514853.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2038..3000
                     /locus_tag="PW101_002483"
     CDS             2038..3000
                     /locus_tag="PW101_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2514854.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3027..3941
                     /locus_tag="PW101_002484"
     CDS             3027..3941
                     /locus_tag="PW101_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2514855.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3967..>4543
                     /locus_tag="PW101_002485"
     CDS             3967..>4543
                     /locus_tag="PW101_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2514856.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVE"
BASE COUNT         1416 a          859 c         1010 g         1258 t
ORIGIN      
        1 taacctttgt tataaaacgc tttattctgt gtaatgtcct tttttattaa gaaaaacttc
       61 catttaggaa gcagagaggg gtattcgatt gtccgataac caattattag aagttcagaa
      121 cctacataca ggattccgac tcaaagatga ctattacgat gcagtagatg acgtttcatt
      181 tactttagac aaaaacgaga ttttagcaat cgttggggaa tctgggtgcg ggaaaagtac
      241 cttggctact acgattatgg gattacttga tcctaataac acaaaaatta caggtgagat
      301 tttatataac gatctgaatt taatcgacct gaacgaaacg ctctataata aaatccgcgg
      361 aaatgatatc ggaatgattt tccaagatcc gttgtctgca ttaaatcctc tcatgagaat
      421 cgaagaccag atcaaagaag gactttccta tcacacaaag atgaatgcag aacagcgtca
      481 agctcgtgca ctagaattgc tagaacaagt agggattcct aatcctgcga gagttggacg
      541 ccaatatccc catgaactat caggcggtat gagacaacgt gtcatcatcg caatcgcaat
      601 cgcctgcaaa cctccaatcg tcattgcaga tgaaccgacg acagccctag atgtaaccat
      661 ccaagcacag attcttgatt tactgaaaga ccttcaggaa gaaacaaaaa cagggatcat
      721 cttgatcact catgacttag gagtcgtagc agaaatggct gatcgagtgg cagtgatgta
      781 tggcggacag ttcgttgaag tagcaggtgt aaaagaactg tttgatcatc ctaagcatcc
      841 ttatacacgc tctttactga actctatacc acaagaagga aatcatgatg cagaactcca
      901 tgtgatcgaa ggtgtcgttc cttctctgaa aaacatgcct cgtaaaggtt gtcggtttgc
      961 accacgtatt ccatggatac cagcaagcga tcatgaagaa gagcctatcc ttcatgaagt
     1021 cgcaccgaac cacttggtac gttgctcctg ctataagcat ttccacttta gagacgaaaa
     1081 aggagaggtg tagatgacag aattaatcac aatcaaagac ctgaaagttc attaccctat
     1141 tcgcagtgga ttcttcaatc gagtaacgga tcatgtatat gctgtagatg gtgtggattt
     1201 tattatcgaa aaaggaaaga catatggttt agtgggagag tctggctctg gaaaatctac
     1261 tacgggaaaa gccgttgtgg gattagaaaa ggtaacagcc ggacaaatca tgtatgaagg
     1321 aaaagatgta acgaagcgaa gcaaccgcaa aaaaatgggt tataacaagg atgtgcagat
     1381 gatcttccaa gattcaatgt ccagtttgaa tcctaaaaag cgagtgttgg atatcattgc
     1441 tgaacccatc cgaaattttg aacggttaag cgatcaagaa gaaaagaaaa aggtaaaagg
     1501 actgctagat atcgttggga tgccggaaga tgcgctatat aaatatcctc atgaattttc
     1561 aggcggacaa cgtcaacgtc taggagtagc ccgtgctgtt gctaccaatc caaaactgat
     1621 cgtagccgat gaacctgtat ctgctctgga tctatctgtt caagcgcaag tattgaattt
     1681 catgaaacga atccagcaag aatacggcct cagctattta ttcatttcac atgatttagg
     1741 tgtagtaaaa cacatgtgcg acaatatcgc aattatgtac aaaggtcgat ttgtagaaat
     1801 cggtacaaga gaagacattt ataatgaccc gcgacatatc tatacgaaac gtctgctttc
     1861 agctattccg caaatcgatg tagaaaatag ggaaagccac aaagaaaatc gacgaagagt
     1921 agaacaagag tatatccaaa accaaaaaga atactatgat gcgactggtc gagtctacga
     1981 tttgcgtacg atcacaacta cacacaaagt ggcgttgaaa gatggaggtg caagctaatg
     2041 tggaaaacga ttctgcgacg tgttttattg atgatcccgc aagttattat tttaagtgtt
     2101 ctgatcttct tattagccca agcgatgccg ggggatccat ttaccggttt gatcaatcca
     2161 aatcaggatc cggcagtgat cgaacagatg cgacaagcag ctggcttgaa tgacccatgg
     2221 tatgtccagt acttccgttg gatcggaaat gctttgcaag gcgattttgg tcaaagtttc
     2281 ttatacaaac gctctgttgc tgacttgatc ggcgaacgga tcatcaacac tttatatcta
     2341 tcgatcttga cagttgtcct tacttatttg attgctgtgc ctctggggat gcttgctggc
     2401 cggtatcaaa attcaatctt ggataagatt gtcgtaatct acaacttctt tagttttgct
     2461 gttcctttat ttatcttcgg cttgatcatg ctatttgttt ttggttatcg tttaggctgg
     2521 ttcccaacaa gtggatcaca aacactaggt ttgcaaggtg gatttatcac tcaatggata
     2581 gaccgtttga aatatattct gttaccatca attacccaag catttttggc gactgccgtc
     2641 acaatccaat atctgcgaag tgaagtaatc gattcgaaat cgctcgattt tgtcagaaca
     2701 gcccgttcca aaggggtacc gacgaataaa gtattcagtc gacatatttt ccgaaatgca
     2761 gcgttgccga tggcttctca aatggggtat gaaatcactt cgttgattgc tggttctgta
     2821 gttatcgaaa agatttttgg ttatccggga gtcggaaaac tatttattga ttccatcggc
     2881 caacgggact ataccgtgat cacatcctta gtattgattt taggaatcgc gacgctagtg
     2941 gggacattac tttcagatat catcatgagt atcgttgacc cgcgtattcg gattcaataa
     3001 aaccaatcag aaaggagaat ggatcgatga gtgaaaaaaa caatacagca gttcaagaaa
     3061 gtattcctcc tatggggata cgaatgattg ctcgagaatt taaaaaagat aaaattgcta
     3121 ttttttcatt aggtttgcta gtggttttat tattagtgat ctttatcggt gcgatgctga
     3181 ccgatcaaga caaagtgatg tatgttagta tcttcgacaa atacgctgca ccaggaacag
     3241 tttcgacaca aggacaaaag ttcattctgg gagcagatga aggcggacgt gatgtattag
     3301 gacaattgat cataggtgct agaaactctg ttacgatcgg atttgcgatc acgatcctta
     3361 cttcgatcat cggggttgga ttagggatca tctctggcta ctacggaggg atcattgaca
     3421 acatcttgat gcggatcgtt gattttatca tgattctacc gatcatgctg atcattatcg
     3481 tcttcgtatc gattatttcc agatacaatg tctggtcctt tatattcatc atgagtgcat
     3541 tttattgggt agctaaagcc cgattattcc gaagcaagac attatctgaa gcaagaagag
     3601 attatgtcag tgcctcaaaa acaatgggaa ccaatgattt taaaatcatg ttccgcgaaa
     3661 tcatgccgaa tctaagttca ttaatcatta ctaatttgac gatgaacttt gcggcaaaca
     3721 tcggtatcga gacaactttg actttcttag gtttcggctt gccggcaaat gtacctagtt
     3781 taggtacatt gatcggttat gcaagtaacg gagaagtctt agtgaatcgg caatggatct
     3841 ggttacccgc atcaatcctt attttagttt tgatgttgag tatcaattat gttggacaag
     3901 cattcaagcg ttctgcagat gcgcgccaac ggttaggcta gtatagaagc gaagggggaa
     3961 ataggaatga agaaaacgat ttttggattt gcagcgctga tgtctgctgt cgttcttgca
     4021 gcatgcgggg gcggaggtac agatacctct ggaaacaact ctggaggcaa cggcaataac
     4081 aaagaggaat taacttttcc aattgcaaca aaaaataatg atgatgcaat cgaagacggc
     4141 gaattagaag ctgcggtcgc aacggacagt cagtttaaag gattgttcca gtgggagttt
     4201 taccaagatt catatgatgc agcttttatg gctccttctc atgaaccgct atttgcaaac
     4261 gatgacgatt tcagaatcac agacgacgga gctgcaacgc ttgatctaga tgaagatacg
     4321 aaaaaagcga caatcaaagt aaaagacaac gtcaaatggt cagatggtga agatgtaacg
     4381 gcggacgatg tgattttccc ttacgaaatc attgggaata aagattacac tggtatccgt
     4441 tatgacgaca ctttccgtaa catcgtaggt atggaagaat atcatgatgg aagtgccgac
     4501 acaatctccg gaatcaaaaa aatagacgac aaaacagttg aaa
//
LOCUS       DANKHL010000204         4487 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000204 DANKHL010000000
VERSION     DANKHL010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.214"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..367
                     /locus_tag="PW101_002486"
     CDS             <1..367
                     /locus_tag="PW101_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2514857.1"
                     /translation="DNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTIVIGLAS
                     IIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVLVICLSF
                     PQIQQKFHLPQKKKGGSVK"
     gene            364..1170
                     /locus_tag="PW101_002487"
     CDS             364..1170
                     /locus_tag="PW101_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2514858.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            1306..2295
                     /locus_tag="PW101_002488"
     CDS             1306..2295
                     /locus_tag="PW101_002488"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDL2514859.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(2612..3136)
                     /locus_tag="PW101_002489"
     CDS             complement(2612..3136)
                     /locus_tag="PW101_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HDL2514860.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            3423..>4487
                     /gene="scm"
                     /locus_tag="PW101_002490"
     CDS             3423..>4487
                     /gene="scm"
                     /locus_tag="PW101_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL2514861.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         1402 a          914 c          864 g         1307 t
ORIGIN      
        1 agataatatg aagatcatcg gttatatgat ttctaatgga tgcattgctc tttctggctc
       61 cttgcttgcg caaaacaacg gctttgctga tttaaattct ggtattggaa cgatcgtaat
      121 tggacttgct tctattatca tcgcagaagt tttgttacgt aaccagccac tatttctccg
      181 tctcttgact gtcgttttag gtgcggtcat ttatcgtttt attttagcgt tagtcttcga
      241 actgaatgtt gaaccaagtg attcgaaact tgcttctgct ttcgtgctag tgatctgtct
      301 ttctttcccg cagatccagc aaaaattcca tttacctcaa aaaaagaaag gcggtagtgt
      361 caaatgaatg acgtcttaac attggaaaat ctccatcata cctttgaaaa aggaacgatc
      421 aacgaaaatc atgtcctgaa aggcattaat ctttccttag atcccggcga cttcgtgacg
      481 atcatcggtg gaaatggggc tggtaaatct actttgctga acagcatcgc cggtacattt
      541 tctgtcgatc agggtcaaat tttattgaat ggaaaaaata tcacgaaaaa atctgttgtc
      601 gaacgttcga aatccatcag tcgcgtattc caagatccta aactagggac agccgttcgt
      661 ttaacggtag aagaaaatct tgctcttgct atgaaacgag gaaaaaaacg cggcttcttc
      721 cgtggtgtca aaccacaaga ccgaacattt ttcaaagacc agctggttcg gctaaatttg
      781 gggttggaaa atcgtttatc ctcagaaatc ggtttgctgt ctggaggaca acgtcaagct
      841 attacgttat tgatggccac attacagcgt ccagaactga tcttattaga tgaacacact
      901 gcagcgcttg accctgctac ttccaaaacg gtgatggaat tgacagaaaa gctgatcact
      961 gagcagaatc tcactgcttt tatggtcact catgacatgg aagacgcgat ccgctatggg
     1021 agtcgactga tcatgctgca tcaaggaagg atcgtcgtag atatccagca agaggaaaaa
     1081 cagcagctca cagtgcccca attattagcg ttgttcaaac aaaacagcgg cacggcatta
     1141 aaagacgatc aagtactttt atcttcataa tcagaaagag gatgggacaa aaaatgaacg
     1201 cccaccctct tcttttttct ctttccttct ttcgtttttg ctttatgtat caaccgttgt
     1261 ttcttcgaaa actattttgt tctctccttc ttcctgatag aatggatggg aaaggagctt
     1321 gatgccatga caacgaaaga caaggtactt caattattga aaaagacacc agattttctt
     1381 tccggagaaa agttggcaca gcaactggaa gtttcccgga caagtatttg gaaagccatc
     1441 aaagaattgg aaaaggcagg ctatcgtttt gagcacgggc ctacagggta tcgttacctg
     1501 ccctctgacg tactagatgc aaaagaaatc cagcaagagc tacggtcgtt tattcctgat
     1561 ttgaaagtac aaacgatgat gacttctgaa tctacgatga aagatgccaa actggcagca
     1621 gctcaaggag attctgtttc gaagttattt gttgctgata cacaagaaca agctcgtgga
     1681 cgttttggac ggcctttttt cgctgaacct ggacgaggaa tctacatgag tctcttgctc
     1741 catccagaca aacggtttga tgaattgcct caatatacga tcatcgcagc cagtgcctgt
     1801 gtgaaagcca ttgaatctgt aaccggtcgt tctgcttcga tcaaatgggt caatgatatc
     1861 tacatggatg gaaaaaaagt ctgtgggatt ttatctgaag cagtcagtga tatggaaagc
     1921 ggcaggatca gtcatatcat cattgggatc gggcttaatt tttctgttcc tcaaaatcat
     1981 tttccagctg atctccaaga aaaagccatc tctgtctttc ccgacggaca agctagtgtc
     2041 acaaggaatc agctaatcag caaaatctgg caagaattgt tcacttatct ttctcaccca
     2101 gaaaaagcgt tggacttcta taaagaaaaa tcattcgtat tgggaaaaac cgtgtcgttc
     2161 aatcaaaaag gaacgactta tgtcggtctt gcaaaagaaa tcggtgatca tggcgaactg
     2221 atcgtccaaa caaatgaaca gaagatgagt ttgtcttctg gcgaaatcag tctcacatct
     2281 atccgtacgc aataattatc agatttcaca aaaggacacc tgatctcata cagtgtcctt
     2341 ttttctgaat tctcttcaaa agactctcct tattctatca gctgacagtt tctctatcct
     2401 ctagaaaaag gtattctaaa aatatctata aagcatggta cactttcttt aaatgaatca
     2461 atgcaaaaaa caagagagtc atcagtttac acgatcaaaa gagatagcag gccgcttcct
     2521 ttttcaatct tatagataga ccatgggtaa cgataaaaac aaaaaagaac ttgcgactta
     2581 ttttgccgta agttcttttt tagctagtgc cttaatctct taatcccaac aaacgtctcg
     2641 catagatcac ctgaccagag tgcatagcag catcatcaat aatagaaacg agacgcattc
     2701 ctcgtgtgac tggtggtgtc cagttctcgt caattacttc atctagctcg gaaggttcca
     2761 gccttcttaa gtaagaaata gcaaaatcag cagctcctcg caagtatcct agcagatctt
     2821 ctatttgatt agcttggatc tgctttgctt gttccagact atgcttccaa tcttgttcgt
     2881 tatctgctat cgtcatcgga aaccggcttt cccagtcttg actatgccaa attggagaag
     2941 ttcctgctaa atcagcaatc tggaaatcta attctcgagc ggtatgccaa gccagccaag
     3001 tcattgattt gatctgcggg gctctatctt ccactggaaa acgattggcc tcctcaatag
     3061 agacgccttc aaacaaacgt tcaaaccgat cgtatgcccg atccacactc tcgcatagca
     3121 tatctaaata atccatttat tttcctcctt tttatttatc ctacttactt tttcctaatt
     3181 ctaggatacc tacttatatg ttactacaca cccttatttt ttacacttta acagtcacca
     3241 tcatcaatag tttggagatt gaaagaaaaa gatagtgtcg aactatcaat tgaacaagaa
     3301 gcaacaatat gaacgtttca ttaatattct ttaaataaac aatgattttt caaacgaaca
     3361 gtttaagact aggagaaaaa gagtataata ggaaaatgtt tgaaaggaaa ggaaggggaa
     3421 aaatgaaaaa aagggcaagt ttactagtcc tagttgcttt gttgttcaat atcttttcgt
     3481 atggtgtggc cgtttcagcg gaaacataca ttcctaaaga ccctgggaat atttataatg
     3541 atctaactgg taactatggc ttgttaggaa ttgctagtca attccacgta tttgctaaaa
     3601 ataagacaac tgtgaatgca catacagacg gaaatatagc aacacatgaa cttgatgcaa
     3661 aaaataattt tggtacagag cttcatgaag gtttattgaa ccaagaaata aattatatac
     3721 aaaaaactga ttctttcgct aattcttcag gcatacctac aggtagcaca agaatgaata
     3781 aatttgtcgt tggcagtaac gtggatgtag cacttcaaga tggtcatcct acagtcaatg
     3841 gatccaggct tgatcactta actccagatg atttttataa agatcgtgct gggcatgaat
     3901 atattgattt cgagcaggaa tttgctaaat tgaatgtagc agcaaatgat cttgcaacga
     3961 tcactccagc taaaacatat actgctgcag attttccaga catgaacaat cggacaatcg
     4021 atttaactga tttatcagat accgggtttt tattggtaaa tatcgatgca gaagtgttaa
     4081 cgatgaatac gccacttcaa atcattaatc caaatgatca agtagttgta tttaatgtca
     4141 tcaacagttc ttctgcattg aacgtacagt caccaatcaa atacaatgat cgttcaaatc
     4201 atgaaacaga agatttttcg gatgcgaata tctcttggaa ttttggaaat gagatgactg
     4261 atttgacaat cagtgctcct tttcaaggaa caatccttgc gcctaatgct actatccgca
     4321 ttactcaaaa ccaagacgga acgattatag gaacaaatgt gattttagat gcagctacca
     4381 atcgttggga tcctaacgaa atatttatca gcgacaatgg tacagatacg acagacacaa
     4441 gcgacagtac agatacatct accacaagtg atagctcaga ttcatca
//
LOCUS       DANKHL010000205         4469 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000205 DANKHL010000000
VERSION     DANKHL010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.215"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            44..838
                     /locus_tag="PW101_002491"
     CDS             44..838
                     /locus_tag="PW101_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2514862.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            851..2617
                     /locus_tag="PW101_002492"
     CDS             851..2617
                     /locus_tag="PW101_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HDL2514863.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            2640..4448
                     /gene="uidA"
                     /locus_tag="PW101_002493"
     CDS             2640..4448
                     /gene="uidA"
                     /locus_tag="PW101_002493"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDL2514864.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
BASE COUNT         1644 a          535 c          901 g         1389 t
ORIGIN      
        1 aaattgcgag cttttaaaac aaaaacataa ataggagaga aatatgaata attggatatt
       61 ttgtattgat tcagatggct gtgttatgga tacgatgact tataaacaca atctcttttt
      121 cggaccaatt gctgcagaga tttttgatgt tcatcataga gaaaaattcc tacaggattg
      181 gaataatatt aatttatatt cagagactcg tggaataaac agatttttag gtttagttaa
      241 aacattagaa aaaaatgatg taacatcgat taatcgatta aagcactggg tatctctaac
      301 aaaagaattg tctaatccat ctttgaagag ggaaatagaa tattctcagt cagaggattt
      361 attaaaagct ttaaaatggt cggaagaagt caatcaacag attagtcaag caaaaggatt
      421 agataaaccc tttttaagtg ctagagatac tataaaagct ctgaaaaaat atgggaagat
      481 aattattgta agctcagcta ataaggaagc tgttcaagaa gaatgggaaa gacatgaatt
      541 attatcatta gttgatgaac tgtgttgtca agataaagga aaaaaagaag atattattcg
      601 aagtgtaatt gaaaatggtt gtgatttgga taagatatta atgattggtg attctcctgg
      661 tgatctagaa gctgcaaata aaaataaagt tttcttttat cctattttgg tcaataaaga
      721 aaaagaatca tgggaaaact taagaactga tgttttattt aaatgtttat cgggagatta
      781 tgtagatatt gaacaaaaat atatagatac tttttggaaa aatcttacta ataagtagag
      841 gagaaagaaa atgactataa aagtagattt acgaaaaaaa ccatataatt tagatgaaga
      901 tgctatttct tggattgaag agacgattga ttctatgact ttggaagaaa aaataggtca
      961 attgtttgtt aatatgggat ctagtcgtga taaaaattac ttgagtaata tggtcaatca
     1021 gtaccatatt ggagctgtac gatataatcc aggaactgct gatgaggtat atgatcaaaa
     1081 ctatatctta caaacagaaa gtaaaattcc tctattaatt gctgctaata cggaagcagg
     1141 tggtaacgga gcttgtacag atggtactga agttggtgta gaagtaaaaa ttggtgcaac
     1201 aaatgatgcg aaatatgctt atgaattagg gagagtttct gggattgaag catcggctat
     1261 tggatgtaac tggagttttg ctcctattgt agatattaat agaaattgga gaaatcctat
     1321 catttctagt cgatcttttg gacaaaaccc tgataaagtt ttagaactta gtttagctta
     1381 tatgaaaggt attcaggaga gtggaatttt gcctgcagct aaacatttcc ctggtgacgg
     1441 tattgacgaa cgagatcagc atctatcttt tagtattaat agtttaggta cggaagaatg
     1501 ggataattct tttggaaaag tatactcagg cttaatagaa gctggattac cttcaattat
     1561 ggcaggtcat atccatatgc cagcttattc aaaacatttt aatcctgatc tgaaagatga
     1621 agatttactt cctgcaactt tatcaaaaga attaatcact gacttactta gaggaaaact
     1681 cggatttaat ggattagtag ttactgatgc tagtcatatg ttaggaatga ctggggcaat
     1741 gaaacgatca gaattattgc ccacttctat tgcagctgga tgtgatctat ttctattttt
     1801 taatgatcca gatgaagact ttaattatat gatggaaggc tatcgtaaag gaattatcac
     1861 tgatgatcgg ttattagatg ctttaaaaag aattttaggg ttaaaagcta aattgggatt
     1921 acacaaaaaa gcaaaagaac aaattttatt acctaagcaa gaagctctgg aaaaaattgg
     1981 tttaattgaa aataaatcaa tttttaaaga ggtggctgac caagcaatta cattagttaa
     2041 agataaacaa gatatttggc ctattgtacc agaaaaatat aaaaggattt tactagtaga
     2101 tgtacatggg gtaaaaggtg ggtttggtga agtagttgct ggaaatagac ctcaagccat
     2161 tgatttattg aaacagattt tgtcagcaaa aggatttgaa gttgaaaaat acatttcacc
     2221 tgaagaagaa ttgaaaggat tatcgcaaga agaaaaaatt aaaaatatag ctggaatata
     2281 tgctgctaag agaccaatta aaaatttaac taaaaaatat gacttgatca ttaatatagc
     2341 taatgttaat ccaggcacag ttcaacgaat tgtctggcca gcaagtaaag gaactccgga
     2401 tataccgttt tacattcatg aaattccaac aatttttgta tctgtacagt gtccgtttca
     2461 tttggctgat gtacctcaag taaagactta tattaatact tatgatggaa aacaagaaac
     2521 tttagaaata ttggtggaca aactaatggg tctttctgaa tttaagggag taagtccaat
     2581 agatgcttat tgtggtttaa aggatacatt tatttaatat ttagatagga gtggtttata
     2641 tgttgtatcc cattatgaca gagacgaggc aagtaattga cttaagcggt ctctggagat
     2701 ttaaattaga tacaaatgat gacttacagg aagaacttgc tttagaacca ataaatgaaa
     2761 aagagagtta tccgatttca gttccctctt cttataatga tttatttgaa acagagaaca
     2821 ttagaaatca tgttggatgg gtgtggtatg aaaaagaagt gactcttcct aaaagattat
     2881 tagaagaaag attagttctt cgttttggat ctgcaacaca tgaagcaaaa gtttttttga
     2941 atggaaagtt tattactcaa cacaaaggtg ggtttacacc atttgagtct gaaattaatc
     3001 ggtttgtaaa agacgggaat aatcgaatta cagttgctgt aaacaatatt attgacgata
     3061 gtactttacc tgtggggatt atgaaggaaa cagtacagga aaatggggat ataaaaaagg
     3121 aaaacttagt taattttgat ttcttcaatt atgcaggtat tcatagacct gttaagttat
     3181 atacaactcc gaaagactat ataagtgata ttactattaa tacaagaata actgatttgg
     3241 atgcaagtgt agaattcaat atcgatacag aatggaccgg aaaaatctta atagcgatat
     3301 atgacgaaga ggctcaactt gtagctgaag ctgaaggaaa gaaaggaatg attcagctta
     3361 agaatccaat tttttgggaa cctctagacg cttatttata tagtatgaaa attagtttaa
     3421 ttgatgataa taaagtgatc gatacctata cagaagaatt tggtattcgc agtgttcagg
     3481 ttaccaatgg caaatttttg attaataata aaccttttta ttttaaagga tttggtaagc
     3541 atgaggattc gtatgttaat ggacgtggtt tgaatgaggc tgtaaatatt aaagatttta
     3601 atttaatgaa atggataggt gctaattctt ttagaacatc acattatcct tactcagaag
     3661 aaattatgcg attagcagat cgagaaggta ttgtagttat tgatgaagta cctgcagtag
     3721 ggttacattt aaatttcatg gccactttat tagatgataa tgtggaaaaa cataatacgt
     3781 ggaaagagat acgaacagaa aattctcata aaacggtgat caaggaattg atagaacgcg
     3841 acaagaacca cccaagtgtt gtaatgtggt ctattgcaaa tgaaccagat tcggattccg
     3901 aaggtgctaa agaatatttt gaacctttag ttgaattaac taaagaactt gatccgcaga
     3961 aaagacctgt aacaatcgtt acttatctaa aatcaacgcc agatgtttgt aaggtaggag
     4021 atattgtcga tgttttatgt ttaaatcgtt attatggttg gtatgtttcg ggaggggatc
     4081 tggcacatgc aaaatatctg ttgaaaaaag agctagatgg atggatggaa agatgtcctg
     4141 atacgccaat aatgtttaca gaatatggag cagatacagt agctggattt catgatacag
     4201 taccaacaat gtttacagaa gagtatcaag tggcttatta cgaagcaaat cacgaagtaa
     4261 tagatacatg tgaaaatttt gttggtgaac aagtgtggaa ttttgcagat tttgcaacaa
     4321 gtcaagggat tttaagagtg caaggaaata agaaaggaat ctttactaga gatagaaaac
     4381 ctaaaatgat tgcttactat ctacagaaac gatggtcatc aatacccaat ttcagttata
     4441 aaaaataatt tggatttttt aagaatgtt
//
LOCUS       DANKHL010000206         4426 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000206 DANKHL010000000
VERSION     DANKHL010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.216"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            26..2695
                     /locus_tag="PW101_002494"
     CDS             26..2695
                     /locus_tag="PW101_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HDL2514865.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2786..3406)
                     /locus_tag="PW101_002495"
     CDS             complement(2786..3406)
                     /locus_tag="PW101_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2514866.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3519..4058
                     /locus_tag="PW101_002496"
     CDS             3519..4058
                     /locus_tag="PW101_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514867.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4055..4393
                     /locus_tag="PW101_002497"
     CDS             4055..4393
                     /locus_tag="PW101_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HDL2514868.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1367 a          773 c          837 g         1449 t
ORIGIN      
        1 catagaaatt tggtggaaaa taaatatgaa aaaaaattta aagtcattct ttagagaaaa
       61 cggacgctac gcagcagcta gtttcctgat tccgtttttg attttagcac ttgtttatct
      121 aagtatcggg atctatcctg gaagcagcag aagtattttg gcaagtgatg ccttttcaca
      181 gttttctaat tttcatgcca gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta
      241 tacatggaat gcttctctag gattgaatta tttatcttta atttcttact atctaggcgg
      301 gatcttcacg ccactggtgt tcttttttcc aaaccagatg atgccagatg cactgtatgt
      361 tctgactttg cttaaggtcg gctgtgccgg attggctttt tggtttcttg cacgtacgtt
      421 caagattcct cgctgggggc atgttgcact tagcgtcagt tacgcattga tctcatttat
      481 cactgcacat tcggagatca tcatgtggct ggatgcattt gtttatcttc cattagtgat
      541 tttggggatt catcgtgtta tggatcttaa acggccgaaa cttttattta tcagttattt
      601 actcttgttt ttatccagtt tttatatggg ttttatgatt ggcgtatttt ctttcttata
      661 ttttatcgcc cgtctattga caaactggtc agaatataaa aaaacgattg ttccttatgg
      721 tatcacttct ttactggcag gcggcgcttc aatgatcatt gtattgccag cagtgctgga
      781 tcttcggtca aatggcgagg cactgagtca gatcactacg ttgaaaacag aagcgacttc
      841 ttatctggat ctaatcatga aaaatatgat tggtgtttat gacacgacaa aatacggatc
      901 gattccgttc gtttacgcag gtctgctacc attagtcctc tgtctgtttt attttgccag
      961 tcggaaagta ccgttgaaaa ataaattttt gtacggcagt cttttcgcca tacttatcgc
     1021 aagtttctat cttgttccat tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt
     1081 tttattccgt tatgcctatc tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg
     1141 ggaaaagata gaacaagacg atcgtgggct gttgttagga atcattttgg tcgtagctgc
     1201 tctctttgcg gtggcatggg gcatcaaacc tgaaggaagc tacacctatg tgacgatgac
     1261 ttcctttgta ttgacagtgg ttttcttagg gatttatgct tttgctgtgg gattttatca
     1321 gcataaaaaa ctttcattca atcatctgag tatattgtta ttattgatca tgtctgctga
     1381 agcactggtc aatacgaaca gtatgatcca tggcattctt gatgattgga attatgcttc
     1441 ccgcagttta tatactgatc cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa
     1501 agattccgac tcgttttacc gcttggaaaa tctggatcca gtatctgcaa acgattcgat
     1561 caactatggt tacagtggta tcagtttgtt ttcttctata agaaatcgta attcttcctc
     1621 ttacctggat aaattaggtt tccgttcaag agggacaaat ttgaatatcc gttataacaa
     1681 taatactttg ctaatggatg ggtttacagg aatcaaatat aacatttcta aaaacgaaag
     1741 cacgttcagt aaatatggct tccgccaagc tggtcaaagt ggcgactata cactgtatca
     1801 gaatgtcaat gcattgccac tagcatttac tgcaccgttg tccgttaatg atgttgagca
     1861 gccagtgaca gataatttga ccagtcagac aaacttgatc aatacattgt ctggtttaaa
     1921 tgaacagtat tttacattct atacacctac tttgaagaat cagttgaata cgacgatcac
     1981 aaagacaacg actggcgtca cttactcaga aacagcagat aaccagccaa aagagctcac
     2041 ttgggaagtc catgtgcctg caaatacaca agcttactta agtttgttcc caaccaattt
     2101 tgcacaatta gaaagttcta gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca
     2161 aatcaacatt tctggtcaat actatgatct tggctattat cctcaagaga caacagtgac
     2221 atttactgca agtttttatg gaacgaaaga agtaagtttc atggagccta aagtcgttgg
     2281 tctagatgtt gtagcctatc aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat
     2341 ggaagccact ggcagaaaag cttctgggac tgtaaatacc gctgaagatt ccatgttggt
     2401 gacaactatt ccttatgaca aaggatggaa agctaaaatt gatgggaaac cagtcaaggt
     2461 cagcaatttc aaagacgcat tcttgatgat aaaagttcca gcaggtaaac atactgtcac
     2521 tttcagttac ttgcctgaag gatttacgat aggtgcaatc ctttttgtcc tatgtattgc
     2581 attgtttatt ctttatgtct ggtaccttga ccgcaatcgc cccttttatg gcacgattga
     2641 tccagcaata gaagaaaaac cgcgaaatac acacagaaga agacgaagaa aataaagaaa
     2701 cgatggaatc tgtgtcactc ttctatttaa atggcaaaaa tttcaatctt taaatgacaa
     2761 aaaccacctg atttttggtg gttttttata tacctatgac taaactatct cttgctgctt
     2821 cgacaacttc tacagtgatc ttatccattt gattgattct taaaatccga tcaatctgcg
     2881 caaataaacg atgaataagc ctaaaatttc cttttgtgat cttaatgata gttgtgatag
     2941 cctcataatc agaaaaatct tctagcttga tatcaattcc taaatctgcc catttgtatt
     3001 ccaaaatatg atgtgtttcg tccttactaa ggttatcaaa ttcatgtgca aatcctatgc
     3061 gagagtaaag ctgcggataa cgagacaatc ttttttcaat accaggcata ccgataagaa
     3121 ccattgccag atcatttcta tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta
     3181 aacgatcaat ttcatcgaca ataattaagt caattgcttc ataggtttta ggcacaggag
     3241 gatcttcttg attgtatttt agtaccatat gcattgcttt agtcagacct attctactag
     3301 ttattgtgtt aatatccgaa ctgacacgag acatcttttc agctggtaca gtataaaaaa
     3361 ctttatttac acttaatatt tcatctgttg catttatcaa gaacaagaaa aagtctgaat
     3421 attcaaaaaa ataacggtgt acatttttaa aaggagatgt ataatcgaga tttagaaagc
     3481 gttttcctca aaatttaatg aattaggaag tgattactat gaaaaaatta tttagcgcac
     3541 tttttattct gttatttggc tttattggta ttgggacttc tggaaatata gttaaagcta
     3601 gcacgatggt tgaacaaaca agtgaaatgg caaaaacttt tagtcaatta acaccagaag
     3661 aacaaagtta cttcaaatcc aaaggattta gtgataaaga tactttttac actcaaaaga
     3721 tagagcctca agatccgaaa acacgttttt ctgcaaatgt attgcggtta accgcatcta
     3781 ctaaaaaaat tactaatacg caggggcgga cagaatatat tataacaagt cccaattcgg
     3841 cgatgtatcg tatttctaca cgcttgagtt taggtagtgc aaaatcatat actcaaacag
     3901 taaatctagc taattcttat ggttatggtg gaggaatgta ttttacattt actgggaaaa
     3961 gacaatatta cagtgtaaaa ttagctatgc aagtttataa tacatttggt gtagggtcag
     4021 tctcttgcac tgcgggagga ttgacaatag gaaaatgata atttcttact atgtcttaaa
     4081 tagaagtgat gatgaaggac tcttcgtttt tcaatcatta catgaggata cgatgctaaa
     4141 agagttttat aatcggatta agaataaaca gatagaaaca aatttatatt ttttaagtga
     4201 ctctatcttg ccttttatta gcaaccctaa aattcttaac ttgttgcaga caaaagattt
     4261 gaaaagttta ccgattattg tcattgatga ggaaattgta aaatatggcg cttatctaag
     4321 cgtagaagaa ttagaaaaat taactaacat tggaatgagt attcaggagg ataatggggt
     4381 acatggaaca tagaatgaat atgcacagtt gagaacggaa tttgac
//
LOCUS       DANKHL010000207         4421 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000207 DANKHL010000000
VERSION     DANKHL010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.217"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(12..482)
                     /locus_tag="PW101_002498"
     CDS             complement(12..482)
                     /locus_tag="PW101_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDL2514869.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="PW101_002499"
     CDS             complement(641..1099)
                     /locus_tag="PW101_002499"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDL2514870.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="PW101_002500"
     CDS             complement(1270..2415)
                     /locus_tag="PW101_002500"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDL2514871.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="PW101_002501"
     CDS             complement(2428..3345)
                     /locus_tag="PW101_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDL2514872.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="PW101_002502"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="PW101_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDL2514873.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1357 a          826 c          843 g         1395 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt t
//
LOCUS       DANKHL010000208         4418 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000208 DANKHL010000000
VERSION     DANKHL010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.218"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            153..1859
                     /gene="efbA"
                     /locus_tag="PW101_002503"
     CDS             153..1859
                     /gene="efbA"
                     /locus_tag="PW101_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HDL2514874.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2017..2919)
                     /locus_tag="PW101_002504"
     CDS             complement(2017..2919)
                     /locus_tag="PW101_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2514875.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2916..3608)
                     /locus_tag="PW101_002505"
     CDS             complement(2916..3608)
                     /locus_tag="PW101_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HDL2514876.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3651..4292)
                     /locus_tag="PW101_002506"
     CDS             complement(3651..4292)
                     /locus_tag="PW101_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2514877.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1360 a          946 c          763 g         1349 t
ORIGIN      
        1 taaaaatttg gatttcaacg atagaaacac tgttttttat gtaattagtc caagattttt
       61 ctagactttt gctttactat tcggccaaat tggtttttct tctttttttc gttataatga
      121 atatacttat gaaaagaaat gaggaatgaa ccatgtcctt tgatggtgta tttacccacg
      181 cgatggtcaa cgaattgcgt gaaacgttac tatctggccg gatctcgaaa atccatcagc
      241 cttatgaaaa tgaaatcgtg ctggtgattc gttcacgcgg caaaaaccat cgattattat
      301 tatctgcgca cccaagttat gctcgtgtac agattaccca aatcgattat cagaatcctg
      361 ataatccgcc taacttcgtg atgatgcttc gaaaatattt ggatggcgct attttagaag
      421 acatcgaaca aatcgaaaat gatcgagtga tccatttcca ctttgctaaa cgaaacgagt
      481 tgggtgatct acaaaatatc atattgatcg ttgaattaat gggacgtcat agtacgatcg
      541 tcttagttaa tcgagaaact ggaaaaatac tggatgcgat caaacacatt ggaagctctc
      601 aaaacactta tcgttctttg cttcctggtg tagaatatgt tgctccgcca aaacaagagg
      661 tcttgaaccc atttagctcc gagaaagaaa aaatctttca gcggctttcc caaactgaac
      721 tagatccaaa agcaatccaa cgtcagtttc agggaatcgg tttcgatatc gctcaagagc
      781 tgaccaaaag attactggaa cgtcctaatg aaaagatggt tgtatgggac gaatttttct
      841 cagccatcag ccatcagcct attcctactt tttacgaaac ggaaaataaa gattttttca
      901 caccgattgc ttatcaggta ttgtccgaac aggcatctgc tgtaacagct tatccaacac
      961 tcagtcaatt attagatagc tattatcacg aaaaagcaga gaaagatcgg gcaaaacaac
     1021 aaggcggaga actgattcga aaaatcgaaa atgaactaaa acgaaataaa aataaattga
     1081 aaaaacgtga acaaacactc aaagaatccg aaaacgctga gaattaccgt agaaatggcg
     1141 aactacttac tacttttctg acacaagtac cacgcggcgc aaaagaagtt gttttaccta
     1201 attattatga agaagatcgg ccaatcaaaa tagcactaga tccggcattg acaccaaatc
     1261 aaaatgcaca gaaatatttc catcgatacc aaaagctgaa gaacgctgtt aagctgattg
     1321 gcgaacaaat ccaagaagcc aaagatgaaa tccaatatct tgaatctgtc ttatctcaac
     1381 tggaaattgc cggcccaatg gatatcgaag caattaaaga agaattgact tcagaaggat
     1441 acctgaaaaa gaagaactta aaaaaacaaa aacgtaagaa accatctcag cctgatcaat
     1501 acttttcttc tgacggaacg ctcattcttg ttggtaaaaa caatcttcaa aatgaccagc
     1561 tgacaatgaa gacagctaaa aaaaccgact attggcttca tgcaaaaaat atacccggtt
     1621 cgcacgtcat cattaaaagt gacaagccct cagatgaaac aattacagaa gctgccgagt
     1681 tagctgccta tttttcaaaa taccgatact ctgcccaagt gccggttgat cttgtacagg
     1741 taaagcatat ccgaaaacca aatggcgcca agcctggtta tgttatttat gaaaaccaaa
     1801 agacgattat cgtgacacca gaagaagaaa aaattacagc tatgaaacaa aacaggtaat
     1861 caatgagtgt ttctagaatc atatttgcgt actttcccac aacagcaatt agttataaat
     1921 tgtttgctac aaaaagagga gttgtgacaa aattctgtca caacccctct tttttgatca
     1981 acggtgttca ataaacagcg ttctcatctg cttttcttag ctctgctggt ccagtaaccg
     2041 agtaagctct ttgataaatc tttgagcgat ttcactgagc ggacgtttcg ccgaataaat
     2101 gatataaaat tcatttagtt cgccttctgc caaagggatt aaccgcaagt gctcgcgaat
     2161 ccctccaatc acaataccag atcctgaagc gtaagcatca gtttcaagaa cgatattcat
     2221 caaagtacct cgatcactcg tataaatcac tgtttcttga tcagggatct cgataaggtc
     2281 ttcataaaaa tacgggaaat tatttccatc ctgtgtaaaa cgaacttgag gataagataa
     2341 caattcttct ttggtgatct ctgcgtgttc agctaatgga tgtccttgcc ttaaaaaaat
     2401 atgcgttttg aattttccaa tgctgcggcc acttagctct ccttgttgta aatatcttct
     2461 gataccagct tgattatgct cattcatata aataatgccg atctcactgt gctgctcttt
     2521 gacttcttcg atcactctcg ctgttgtcgt ctccatgatc cgaaattgtt tgatctcgtc
     2581 tttgaattcc tcgagtagct gtgcggtcaa aggacctaag aaatcataat gctgggcagc
     2641 gatcgaaaaa ttgtctctct tttcactcgt tagatagtga gtttctaatg tatcaagctg
     2701 gaccaatatt ttctcggcac gatttaagaa gtcttgccct tcttctgtta aatatgcccc
     2761 tcgattcgtt cgagtaaaca gcgtgatccc taactcggtt tctaattctt tgataccagc
     2821 tgaaagactt ggctgggaga caaacatttt ctttgccgct tctctgaaac taccattgtt
     2881 tgctacagcg accacgtatt tcatctgctg aatattcatt gctcaaactc ctcgaaataa
     2941 taaatttctc ttccattcaa gggctggatt ggtcggttgt tcggtcgagc atactgacca
     3001 tcgaagggga gaagaaactc ttggttcagt tttcctacat gatccattac aaaccattgg
     3061 attggcccgc tggtaggcgg agttgtcaat gagccaatat aatggaaatg gcttttttgc
     3121 tccggaaaga acacatccgg atttacccaa tgctcatgtt tttctaagtt ccaactttcc
     3181 ccattttctt gattatacag ccaaccttcc tgtaccatta gctggtacat gacacccaca
     3241 actagattct cttttttgct gttcatatga acaaaatgaa attcaatatc ttcctgtttt
     3301 ccgttgatga tatgttcaga aggcaagtgg aaatgaatat ctgtcaaata atagcgttct
     3361 ttcttgaact ctacataact gagttggtca aaaggtacta aatgaatcgt gtttttgaat
     3421 tccttgtcag taaaccgttg cttttgataa tgaaaggaaa gcgtgtctcc ttctattttg
     3481 gtttcttccc ccttggtcaa atgaataggc gactgcaatg gaaattccgc acctctcgca
     3541 aaccaatcac ataaactcgc ccaattttcc ggacctttgt ctccagaata gccccattcg
     3601 acatccatat tctttttcaa ctatacttcc ccctgcatct catatcattt ttatttttct
     3661 tctttcttta caatcgttga gacggtttct aaaaacgcct cttttccata aaattcctgg
     3721 ttcaatagtt ccggaaatat ctctttaaag aaatactgaa cagaaggccg atgttcaaag
     3781 cttttaatcg tagtcactgc ctcaatgaac atctccttat aaactttttc cgggttattt
     3841 ttgatgattt catcaaaaga ttcataaaga aggtcaacct tatctgaaat ggctaatagt
     3901 tctccttcta cggtttcatc ttttccttcg aacaaccgac ggcgataaac ttcttggaac
     3961 tcagctggaa tctcttggtt gatgaattcg tccgtcatct tttcttcaac tgtttgcagc
     4021 atccctctta actctttgct tgcgtatttc accggcgttt tgatatcacc aataaaacgc
     4081 tctgtgtagt catgattcaa tgatttttca tagagcagtt tccaatcgat cggttgtcca
     4141 ttcgcttctt caatgtctcc tagaacttgc gcaatagatg ctacacgata cgaatgtgct
     4201 gcaactgtat gctctgtata tttaaaaaat ccaggtgccc ttgtgatcgt ctctaaattg
     4261 ttcaatccta aaataaattc attcaacccc ataaaagtta cctccctttg agatttcttt
     4321 ttccgaatat catatcatta aataactgat attcgcaaca tttccgctct ttaccatttt
     4381 tgaaacagct cccttaccaa aaaatagaga aaatctat
//
LOCUS       DANKHL010000209         4416 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000209 DANKHL010000000
VERSION     DANKHL010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.219"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            59..913
                     /locus_tag="PW101_002507"
     CDS             59..913
                     /locus_tag="PW101_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL2514878.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="PW101_002508"
     CDS             1010..3259
                     /locus_tag="PW101_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL2514879.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..>4416
                     /locus_tag="PW101_002509"
     CDS             3262..>4416
                     /locus_tag="PW101_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2514880.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1556 a          625 c          806 g         1429 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga tatcggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acgggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actaga
//
LOCUS       DANKHL010000210         4337 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000210 DANKHL010000000
VERSION     DANKHL010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.220"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(58..579)
                     /locus_tag="PW101_002510"
     CDS             complement(58..579)
                     /locus_tag="PW101_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL2514881.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            777..>4337
                     /gene="nifJ"
                     /locus_tag="PW101_002511"
     CDS             777..>4337
                     /gene="nifJ"
                     /locus_tag="PW101_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDL2514882.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESID"
BASE COUNT         1347 a          808 c          961 g         1221 t
ORIGIN      
        1 gtgtttagag cgtgagacaa aagtgttctt tacttttgtc ctacgctcct tattttttta
       61 ttttgtttgg atcaattctt ttaattcctc tattgaaata gattcatcaa agatttcttt
      121 acccaagatg acagtcggaa caaatcgaat atgtgcttgc tcagcttcgt tgatgacagc
      181 ttggctcagc tgttcgtctt tttgttctgt cagctttagt ttatctactg caaaatccgc
      241 tacttcttgg agagaaagat gtttccactc gtcttgagta tcgaatattt gtttgatctc
      301 tttgatcgct ttttgaccat cgctgattgt gagataccgg tgcatcacgt ttcctcgctg
      361 caagctttct ttttctttat cgaacaattt gatcactcgc tgcaattgcc cgctttggac
      421 tgcttcttct aatagctcat aagattcttc aaaccattgt cggcaatacg gacaagatag
      481 gttgatgaat tcgaccattt ttttaggagc attcgatgaa ccataaagga ttcctttttg
      541 tgtatttgtt ttcgttgcat caatcactga tatatccatg ttaaaacacc tctttcgctt
      601 atagtttata catgtctgac aaaaaaaggc aacgatttag actgtttgtc taaaaattag
      661 atgaccaatt caaatagaca tatgtgaaat atttttataa ataaaagaaa aaatggaatg
      721 ttttacgtta taatggaaca gataataaga gagaatatga acaaggatga tgaatcatgc
      781 gaaaaatgaa aacaatggat ggcaatacgg cagccgcata tatttcttat gcatttaccg
      841 agattgctgc aatatatcca ataacaccca gttcgaccat ggctgaatta gtagatcaat
      901 ggtcagcagc aggaaaagaa aatgtattcg gtcaaccagt aaaagtagta gaaatgcaat
      961 ccgaagcagg agcagctggt gtggtccatg gatcattgaa aactggtgca ctgacaacta
     1021 catataccgc ttctcaaggg ctgcttttga tgataccgaa tatgtacaag attgctgggg
     1081 aactactgcc aagtgttttc catgttgcta gtcgggcagt gacgacaaat gcgttgaata
     1141 ttttcggaga tcacggagac gtgatggctg caagacaaac aggatttgct atgttagctg
     1201 aaagcagtgt acaagaagtg atggatttag cacctgttgc ccatcttgct tctattgaag
     1261 gagaagtacc ttttttgaat ttctttgatg gattccgtac gagtcacgag attcaaaaaa
     1321 tcgaagtttt ggaatatgaa gaattagctc ctctgatcaa tcaggagaaa ttagcttcat
     1381 ttcgtaaacg agggatgaat cccaatcatc cgactgttag tggtacgaac cagaatccag
     1441 acatccattt tcaacaacgg gaaacgatca acccctatta tgataaatta cctgaaatcg
     1501 ttcaaaaata tatgaaagag atcaaccgat tacgaggaac ttcttatgac ttggtgactt
     1561 attacggagc agacgatgcg gaagaagtca ttgtttctat gggatcagtt gcacagacga
     1621 ttgaacaaac gattgattac ttgaatgcaa acggacggaa aacaggtttt ttgaatatcc
     1681 atctttatcg tccttttcca gtggaaacat tcgtgcaaaa gttgcctcat acagtaaaaa
     1741 caattgctgt tcttgatcga agtaaagaac caggggcagg tggagaacct ctcttattag
     1801 acgttcaaag tgcgttatat gattctgaac ttcgcccggc tgttattggt ggtcgctacg
     1861 gattggggtc taaagatgtg actcccgacc aaatctcagc agtttttgat gaattgaaaa
     1921 aagacccaag catacgcaaa aaacggttta ccatagggat cgtggatgat gtgacttatc
     1981 aatctttgga accaaaggaa agtctggatt tgacagaacc gcaaacattc caggccaagt
     2041 tttggggatt tggatctgac ggaacagttg gagcaaataa atcagctatc aagattattg
     2101 gagatcacac cgataaatat gcgcaaggct atttttatta tgattcgaaa aaatctggcg
     2161 gtctgactgt ctctcatctt cgttttggag atacgccgat acgttcagcg tatcttgtcg
     2221 agcatgcgga ccttgttgcc tgtcacacac cagcttacct gcatagctat gacttggtca
     2281 aaggattgaa gccaggtggc atttttttat tgaatactct ttggagcgac gaacaattag
     2341 aaacgcatct gcccttaaaa ttaaaacgtt acttggctga aaataacatt agattctata
     2401 cgatcaatgc catgagattg gcccaagaag tcggtttggg acgccggatc aatacggcaa
     2461 tggaaacggc tttttttaaa ttagctgaca tcattccttt tgatgaggtg cttccgctgc
     2521 taaaagaaga agccctgaaa agctatggac ataaatcaat ggctatcgta gaaaaaaaca
     2581 tacaagcaat cgatcggacg accgaactgc ttcatcaagt cgcgatccca gaccattgga
     2641 gaacgttgga gatgcctgaa aaaatcaata aaaggaacac tggttatata caagaaatac
     2701 ttgaaccagt gaatgcacag gaaggaaatc aactctctgt tggtacactt gtagcaaatg
     2761 gaatgacaac tggcgaaatg cctttaggaa cggcttctat ggaaaagcgg ggcgtcgctt
     2821 tagaagtacc tgagtggatt agtgatcgct gtacgatgtg taatgaatgt gcctttgttt
     2881 gtcctcatgc tgccatccgt ccatttttag cagatgaaga ggaaatggaa gaagcgccag
     2941 aaggatttat cgtgagagaa atgcgaggcg cagacggcgt aaaataccgt atccaagttt
     3001 cagtggaaga ctgtacaggt tgtggtctgt gtgtagaggc ttgtcccgca aaagaaaaag
     3061 ctttagtgat gaagccttat gaagaacaga aagaacaagc tgtgaattgg gcttttgcaa
     3121 tgacattaag acaaaaagca aatccagtca aaggaaagaa tactgtcttg tctacacaat
     3181 tcaatcaacc gcttcttgaa ttctcgggcg cttgctcagg ctgtggcgaa acaccttatg
     3241 taaaattatt gacacaaatg ttcggtgatc gaatgatgat cgcgaatgcg acaggctgtt
     3301 cttctatttg gggcggtgct tctcctgctt ctccttatac gaccaatgaa tgtggtcaag
     3361 ggcctgcatg gtcaaattct ttattggaag ataatgctga atttggttat ggtatgcttc
     3421 tagcggcaca gactcgtcgc gaggctttgg caagttcaat caaagaagta ttgccaaaag
     3481 tatcagaaga actacgagaa ttgatggaag actggttggt acacatgttt gagagcgaag
     3541 gaacgcagca aagagcagca aaactgcaag ctgcgatcga aagcgaacga tcaattgtcc
     3601 ctgaactgga ggatatatac aagagaaagg atctattcgt aaaaaacagc caatggatga
     3661 ttggtggaga cggatgggct tacgacattg gttttggcgg gatcgatcat gtacttgcca
     3721 gcggagcaga cgtgaatatt cttgttttgg ataacgaagt ctattccaat actggcggtc
     3781 aaacctcaaa agcaacacca gcttcggcaa tcgctaaatt ttcggctagt ggaaagtatg
     3841 cttcaaagaa agatctagga atgatggcga tgacatatgg aaatgtttat gtggcgcaaa
     3901 ttgcttcagg tgccaatcaa atgcagacga taaaggcttt tgaagaagca gaaaagtatc
     3961 ctggaccttc tttaatcatt gcctatacgc cttgtatcac acatggtttg cttggtggaa
     4021 tgaaagaatc gcttaaagaa gcaaaagaag ctgtttcttc tggctattgg tcattatatc
     4081 gatataaccc tgcaatggtc gaaaaaaaca agaacccaat gactttggac tacaaaaaac
     4141 ctgatttctc tgctatgcct gatttcatga ggaaacaagt tcgcttttcc tcgctagaga
     4201 acgcaaatcc aaatcttgca aaagctttat ttgaaaagac tatccaagac gctaaaacac
     4261 gtttttacaa ctatgctgca ctgacgggag aattagaaaa aatcaagaaa cgacttgaac
     4321 cagaggaatc tatcgat
//
LOCUS       DANKHL010000211         4335 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000211 DANKHL010000000
VERSION     DANKHL010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.221"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            127..579
                     /locus_tag="PW101_002512"
     CDS             127..579
                     /locus_tag="PW101_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514883.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="PW101_002513"
     CDS             593..1282
                     /locus_tag="PW101_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514884.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="PW101_002514"
     CDS             1407..3758
                     /locus_tag="PW101_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HDL2514885.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="PW101_002515"
     CDS             3838..4233
                     /locus_tag="PW101_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514886.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1526 a          548 c          867 g         1394 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatt
//
LOCUS       DANKHL010000212         4309 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000212 DANKHL010000000
VERSION     DANKHL010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.222"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            57..1358
                     /locus_tag="PW101_002516"
     CDS             57..1358
                     /locus_tag="PW101_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2514887.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1385..1645
                     /locus_tag="PW101_002517"
     CDS             1385..1645
                     /locus_tag="PW101_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL2514888.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2644..3879)
                     /locus_tag="PW101_002518"
     CDS             complement(2644..3879)
                     /locus_tag="PW101_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL2514889.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1256 a          690 c          888 g         1475 t
ORIGIN      
        1 tctttgcgtt gtcatgcaat gaatggttca tattttattg aaaaggagtg gttttagtgg
       61 aaaagctgat gaaatgggtc gaagagaaac ttgtcccacc aatggcaaaa attggtacac
      121 agcgtcattt attagcaatt cgtaatgggg ttgtttcaac attgtcattg attttgattg
      181 ggacattttt tatggtcttt atcaatttac cgtttcctgg atggaatgaa tttattgccc
      241 cttattcggc aacgattgtt ttacccttta ggatgacgat gggattgatg gctatttatg
      301 caacttttgt tatgggttcg gatctagcta aatcatatgg attagatagt gtgacaggtg
      361 gtatattatc tttaggaacg ttctttatgt tacaagttcc tgtaaacgtt ttaactcctg
      421 aagaggcacc tcttggctgg gtcttaccta tgagttcttt gggagcatct gggatgtttg
      481 cggggatctt atctatgatt tttgcagtgg aagtatatcg tttctttaaa aatcgaaata
      541 ttactatcaa aatgccagaa caagtgccac ctgctgttgc ccgtagtttt gaggcattga
      601 ttccaggtgc agtcatttta acaattactt ggctgattcg aagcgtcgcg ggctttgatg
      661 taaacgctgc cttattatcg ttattcgagc ctttaaccaa tattttaggg aataatttgc
      721 ttggtgtttt actaccaatg tttttgatcc atttgttgtg gtcatttggt atccatggca
      781 tgagtattgt aggggctgtt gttcgtccaa tgtggctgat catgttggat gagaatgcaa
      841 aggcattggc agatggtaca gctgccaccc aattacccta tatcgctcct gaacagtttt
      901 atcaatggac ggtaacaatt ggtggtgcag gagcaacgat cgtggtatct gttttgttta
      961 tattcttctg taaatcaaaa ttcttgaaag aggttggtcg tttttctatt attcctggta
     1021 tcttcaatat caatgagccc ataatttttg gagctcctat gatgttgaat ccttacatgt
     1081 tcatcccgtt caacttagtt cctttagttt tgactattat cagttatttt gcagtcaaat
     1141 tagaaatggt caatagattt actgttttgc caacatggac attaccagct ccaatcggcg
     1201 gttatctttc agcaggaaat gactggcgtg ttgttatctt gatcatgatc aacacattga
     1261 tagcatttat tatctattat ccatttgtaa aagcttatga taaaaagatg ctgtcagatg
     1321 aacaaagtcg tggagaaaac gaagtccaga ctgtctaagt ttggttttta gaaggtggga
     1381 aacaatgtat tggctgattt tattttttgt ttttatcttt ttattaacgg ctagtcacct
     1441 tatattgaat atgctagcta catatcatat tcaaatcaat cgatggattt gggcactggc
     1501 atcatttttg atcgtgattc ttccaaagat cattgtaccg catatgaatg ttttgttttc
     1561 atgggggaca tatgtacttt gtggaatttt tgctatcaat tttatgatcg agcagcatag
     1621 atggtttgtc acatcgaagc tttaaatgtt ctaaaagtgg ttgtaacaga agtggttagc
     1681 ttcaagaaat aagaagaaat ttacgaaaat tgcttcttaa atttttgtga aatgttggct
     1741 tatttttcgt ttggttgaac ttattaccgc agatgcaagc ttttgaccac cgtttattcg
     1801 gaatatggga ctgtgacaag gcttttgtca cagccctttt ttacataaag attattattg
     1861 ttttaactgt tttggcagaa agaaggagga aaaaatggaa attttatcag caatggattt
     1921 agaattagta gatctactac caaagtgatc aagtcaaata tagatttggg agcttacaac
     1981 catatagtag gggctgcagt atggtgtatg aatggtaata tgtatgatgt gagtgtttat
     2041 tcaaatcatg aaacgtgtat agaaataatt gtgctgggaa tagcaatcag tcatggtgaa
     2101 cgtgactttt agtgtatcgt tgctgacgac atagaacatt ctgacatgat ctactcaata
     2161 tgtggggatt gctggcagtt tatataggag tatgcatcgg attgcgagat cattgtctca
     2221 acagaagagg gaggttgatt atcaaagata aagtggtaaa tcctacattt gatttagtaa
     2281 ttgttgaggg gactagcggc acaaatctct acaaagcgat gcttctagat ctatatgaca
     2341 cattttaaga aaatgtattt gcctaattat tatggtcttt cttttgaaga aacgttgaga
     2401 tgatccaacc agagaaaggt gtcaaagaat ttgactaaga ataaatggca gacgctacct
     2461 atgctcattt ggatagcgtc agatctttta ttttaaggat gattttgtgt tttttagaca
     2521 ccgtaattta agtaactcta acttttcaat ccactattta ctttttttgt attttcactg
     2581 tctgggtctc gaatagcgaa attactgatg agtctctatt aaaaaagctt tttctacttg
     2641 aaactatatt gggtggttgc tcaggaaggt ttcgatttct tgaattgctt gttgtggacc
     2701 atttattgga aaaacggctt gcagcgtgcg tgcctgctga cgaatcttgg gtgaagaaac
     2761 ttcgcgcaga gcaatctgca gttttctttc agtcactggt tttgtgaggg cgattgcgtt
     2821 tccgtatttt acacaatagt caatattaca gctttgctca aattgcatcc ccacaccgat
     2881 aaacggtttg ccggattcac aggcagtttg gacggtccct tccccgccgt ggataaaaga
     2941 aaaatcaatg aattcatgca aaagatgact agacagatat tctgttatta aaagatcttc
     3001 tgagtcagat tgaatgtcat caagcagata gtcaataggt gcaataaccg ctagatccgg
     3061 ttgtttttgt aagaagaaca aaattttgcg taacatacgc ggattgcctg aactacccat
     3121 tgcgaaatag ataatacgtt ttctttcttg ttgctttcgc tgtataaaag ttagcacttc
     3181 ttcaggcaag gaagtactga gcttagcaaa aattggacca acgattcgat aattttccgg
     3241 cagctgttct tcttccaaaa atagtttagg cgttgtgatg agatttagat cagcatccat
     3301 catttgtaaa cttgtttttg gtaaaggaag catttctttt tgtgcagcgt gtttgaaggc
     3361 attggggatc catcgggtat ttactatacg cttttttgtc cagtgccaaa ggggattgct
     3421 taaagggccg aagaaacgta actcgtgggg catcagcgca tgctctgatt gaaaaaaagg
     3481 ttcactcaaa gcatagggtt taacatatac taaaggaatc tttttattgc gggccgataa
     3541 aaaaattgtc acattcgaac ctgtcacaat cagtcgaggc tcaaacttag caatcagact
     3601 ctgttcacta gtgatacgag aggcaaccat ttctttggta aaagggtttt taagtgtacg
     3661 caactggtca aattttatga tttgttcttc ttgttttttc gtaaggatcg gcgctagtaa
     3721 atgatactca aagccacttt ctgtgatcaa aggagcaaaa gttttagaaa agccaaagaa
     3781 tacacagtaa taacggtctt tcatagcttt ggcaacctca atcattcgtg ttgtctcagc
     3841 taaattgaat actgcaggag caaaaagaat tgtcttcatg aaatcctcct tattggaatg
     3901 agtctatccc ttcgtttctt actagtatac taaaaaacac agcgttcgag tagcctatgt
     3961 atattttgaa tattaaactg tctctcgctg ttttcacaac ttctaaattt aaattttgta
     4021 atcaagttga aactccaatt ttcaaaaaaa cacgcaacaa ttttttgatc gatttatatg
     4081 ttgtgccaga taaatacgag aagttgtgtc attaaaaata aacatgagaa cataaaaaaa
     4141 taaaataatt atattaattg ataggtgtgc tgtcgtcatt atacacacgt aagaaagaaa
     4201 tagttatgtt taaaaatatt cttagacttt ctcgccatga caattttcac tatgtgttat
     4261 aactgtaaac gataattatt cagataacac aaaaagaaat caaggagca
//
LOCUS       DANKHL010000213         4253 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000213 DANKHL010000000
VERSION     DANKHL010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.223"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            75..503
                     /locus_tag="PW101_002519"
     CDS             75..503
                     /locus_tag="PW101_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2514890.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="PW101_002520"
     CDS             490..732
                     /locus_tag="PW101_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2514891.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="PW101_002521"
     CDS             complement(1032..1247)
                     /locus_tag="PW101_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514892.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="PW101_002522"
     CDS             1354..1668
                     /locus_tag="PW101_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2514893.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="PW101_002523"
     CDS             1772..2950
                     /locus_tag="PW101_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2514894.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            3353..3646
                     /locus_tag="PW101_002524"
     CDS             3353..3646
                     /locus_tag="PW101_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDL2514895.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1452 a          641 c          821 g         1339 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
     3121 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
     3181 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
     3241 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
     3301 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
     3361 tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt
     3421 aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg
     3481 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
     3541 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
     3601 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
     3661 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
     3721 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
     3781 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
     3841 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
     3901 acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa
     3961 gataacaaga tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa
     4021 acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac
     4081 cattttttaa gtgaatacaa gatcagctaa ttgaacaccg tttattcgtg atgagggaca
     4141 caacactgca gtcgacacat ttctctttta tgttcaaaga taacaaatat ttaaaacaaa
     4201 gaaaaatagt agataatccc gtcctaactc ttttattctt ccctcatagc ttc
//
LOCUS       DANKHL010000214         4240 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000214 DANKHL010000000
VERSION     DANKHL010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.224"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            39..476
                     /locus_tag="PW101_002525"
     CDS             39..476
                     /locus_tag="PW101_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514896.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            571..1455
                     /locus_tag="PW101_002526"
     CDS             571..1455
                     /locus_tag="PW101_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL2514897.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1677..>4240
                     /locus_tag="PW101_002527"
     CDS             1677..>4240
                     /locus_tag="PW101_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514898.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIP"
BASE COUNT         1386 a          713 c          959 g         1182 t
ORIGIN      
        1 agaaaacgat attaaaaaat gcacgtagaa gtagataaat ggagtggttt tgcaaatttc
       61 ttttatttct agaagttatt cacgtatacg gatttgtttt tctcacaaat aaaaaggcag
      121 gaagtacaat ggatcatttg tatataaaat atattcatca attttccgtt ttggtattag
      181 gcacactaat tatagcgggg attgttgcat ttattattca aggtcgtgac atattaacag
      241 tacatcaaga gtttttcagt tatgcatttg gtattcctct gatcgctata aggtctgata
      301 acaaaaagtg gaagaaacgc tattacattc tcttgctatt tttgtgtttt tatctgccac
      361 ctaagatttt tactttatta ggtatacaat gcctatcatt ttcttttatt tcctatatca
      421 ttgttttaat tttcctcagt ttcatagtat gggtatttta tcttgtcaaa aaataaatga
      481 atgtttcatg aacagtactt catcacagcc taattttttt ctttatgcta tcgttatatt
      541 aaagaaatca atcagaaggg aaagaaagtg atgaaaaaag ttttattagt ggtaaacccc
      601 agttcaggcg gtgaacaagc aaaggaattt gagcaattag cgatagcaaa attggaatcg
      661 gtgtttgatg aagtggtcgt cttgcataca aaaaaagcag gggatgcaaa aaactttact
      721 cgcgaagcgg ctacggaagg gtatcatagt gtgtttgtca tgggcggaga tggaacagtc
      781 aacgaaggaa tcagtggaat cgctgaacaa gaacatcggc caaatttcgg atttttccca
      841 ttgggtacgg tgaatgactt ggctcgagca ttagggatac cgctagaacc tgaggaagcg
      901 atcaatcatt tttcgattga atcagtcaag ccattggata ttggaaaaat aaatgacgac
      961 tattttatga atgtcgtcgc aattggctct atccctgaag caatcaatga tgtagatgcg
     1021 gagaagaaga cgaaatttgg aaaactagct tatttcatgt caggaatcaa acagttagct
     1081 tccacacaat cctactcgtt tcacgtagaa gttgatggaa agaaggaaga aattgagagc
     1141 agtatcttgc tgataggact aactaactca gtaggcggtt ttgaaacatt acttccaaat
     1201 gcaaaagtgg atgatggtaa gctccatttg gtttatttaa aagattcttc tttgatcgat
     1261 acgcttaaaa cattgccaga cctattaaaa ggagtagatg aatctacgaa aaatctagcc
     1321 tatcttactt gtgagcaatt agttgtgtca ttagctgatg aggaagaatt gacgacgaat
     1381 gttgatggag atgaagggga taaattacct gtgacaatca aagtgttacc ctcacattta
     1441 caggtttatt gttaaaatgg aggttgtgac cgaagagttg tcaggaggac atcggttgtt
     1501 cggaataagg gactgtgaca agacttttgt cacagccttt ttttgtacaa aaaagaaatt
     1561 ttcacctaac agaaaaattt gccataaaaa tacatatttt aaatatgaaa gtacaattac
     1621 aaataaatta aaacgttaaa ataaaattgt caaaacgtca gagaggatgg tggagaatga
     1681 atcaatatgt aaaaaagctg attgcgtcga ttttgtttgt cttccctctc ttgatggtga
     1741 catccgtttt tgccgatgta gagaaaacaa gcgaatcaac gcaatcagag aatcaaagtt
     1801 ttgacaagac tactttttca gaagaatcaa ttacttctga aactacagaa tcccaaagtg
     1861 aggcgcctac ttctaataca aggcaagaag aaaataagac aagtgagacc tcagaagtag
     1921 ctgaacaaac gtctgatagc agtgaagagg tacaggttag agttgtttca gcggatgggt
     1981 acagcgaagc tgccacaaca gaagaactga cgcagcttct cgcagacgaa tcagtagcta
     2041 agattcgttt aactcagcct ttgactttag acagggaatt ggagatcaag cgagatatcg
     2101 ttatcgattt tggcggactt gctcataatt tcggcacaca tcacatctat atcaatgaga
     2161 cacctcagtc gatcgagttt cagaatttta aaggaacggc agctcatcca ggtgggttga
     2221 taccaaatat cgatggaaat gccattatca ttgcttatat gtccgatttt tggggaaaca
     2281 gatatcattt tacgggagaa ataaaattta ccgggacact tgacttatat gatggttcta
     2341 aattaggcct gatttacgcc cctcgtgcaa ctgtcacatt agatggtgtt tccggtgtat
     2401 tggatgtcca accagtgaaa gaagggatga atgccgctcc gggtaaagct tatttttgta
     2461 gaagctatca gctaaatgta atcaatggta gtcagcttta tggaccatat ctaggaaaat
     2521 tctatggttt tctagattca ggtgatgaga cgggcagtgg aaaaacagca ccaggactcc
     2581 atatcttgag tggttcaaag gtctctcttg actatgatcg ggcagatggc gtcagcgatg
     2641 aaggagaggc tgtcgatacg ctacccggga atgtcacatt taaagtcagt ggctcaggaa
     2701 gtgaattttc ggtaaataca aagatcaata ttaccaacga caacaatcgc ggaattatcc
     2761 aaatgagagg ctcaggatca agagtcgctg tgtcgaatga tgggaaaatc acaatcaata
     2821 cagcgactac tggcggattt cgcctgcaag gagattctag tcaaatccat gtatcaagtg
     2881 gtggacaaat ctcggtcaaa atggctggag atggcgacca acctggaaac aatgggatgc
     2941 gttttgtagg gaaaggactt tctttgatcg tagaaggagc cggatcagtc atcgatattg
     3001 ataagcaaag tggtcgttct agcgcagttc gttttgagaa cggtacgcaa aaactaacag
     3061 taactaatgg aggctcattg aaggtaagaa atgcagggga tggcaaatct tatgtgaaca
     3121 gactcaatca aggaaaccaa gcgatccaat tttatgacgc ttctggtttt ctagaaagtc
     3181 cagggtcagc tgattttacg gttactggag aaaaatcaaa tatcgatata cgcgcagatt
     3241 ccggagctac cgtagataca actggagata ttgatttgaa ctttatggct ggcgaaaaaa
     3301 cttatttagt catggtcggg aaaactgggg aagataagac tggcatcatc agtggggatg
     3361 tgttgaatgt agacttagaa tctcctacct atttcgattt ccaaaacaaa cggatcggaa
     3421 gcgagactaa tggcataggt ggctgggtat ttcaaggaaa tggcgattct tctttgaaga
     3481 tcgctcattc agaaattgct ttatggcgaa aggcaggagt aacgttcgat aactttgaaa
     3541 aagatccgga ttatttttct gatatgacag acttatcgat caatggaacc gacttaggta
     3601 attttgtatc aagccctagt gcaggtttaa caaatgaatt tgctcagaca ggtgccggaa
     3661 tgaaaaatta taatcgaatc agtgccaata accagcttcc gatgattgaa tccttaagag
     3721 taccaacgaa tgcagataaa aaaatctatg ggcatgtcgt tgttcccgaa ggtgtcgagg
     3781 caaagccaag agatgcttgg acaggcgaag tagaagtgac tttacgtatt acttatgcag
     3841 atacgacgaa atcacctgta gaaattactg ctctcacaca aggacaaaca gatgaaaatg
     3901 aagggacagg atataatccg tggggagaag gcgaacaagg cggtttgttt gaggttcaag
     3961 tacccaacga agaaagacta acaacggggg atcaagtgca aatagttgct gctaaaaaag
     4021 taagaggcgg ccaagaagtg acagaactgg actctgccaa aacgacagtc gatgtgattc
     4081 ctcctgagcc tgctaaatta agtaccacat tgatcaatgc agcttctcgc tcaatcacag
     4141 gtacaggcga agcaggagct ttggtttcat tgaaaaaaaa tcaagctgtt ttgtccgaaa
     4201 caactattga tgatacaggt aaatttgttc tttctatccc
//
LOCUS       DANKHL010000215         4204 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000215 DANKHL010000000
VERSION     DANKHL010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.225"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(66..545)
                     /locus_tag="PW101_002528"
     CDS             complement(66..545)
                     /locus_tag="PW101_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HDL2514899.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            904..1686
                     /gene="rpsB"
                     /locus_tag="PW101_002529"
     CDS             904..1686
                     /gene="rpsB"
                     /locus_tag="PW101_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDL2514900.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            1785..2666
                     /locus_tag="PW101_002530"
     CDS             1785..2666
                     /locus_tag="PW101_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDL2514901.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            2802..3524
                     /locus_tag="PW101_002531"
     CDS             2802..3524
                     /locus_tag="PW101_002531"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDL2514902.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            3527..4084
                     /gene="frr"
                     /locus_tag="PW101_002532"
     CDS             3527..4084
                     /gene="frr"
                     /locus_tag="PW101_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDL2514903.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
BASE COUNT         1510 a          736 c          896 g         1062 t
ORIGIN      
        1 tattcaataa ggaaaatatt gaaaataaaa aaacacgcat tccatattag gaatacgtgc
       61 tctgattaaa aaagtgatac ttcagttgga tggtgaagaa attcttctac tcgttttgcg
      121 tcctcctcat tttccgagat gaccatgatc gtatcgaatc cagcaattgt actgacaata
      181 gagggaggtt tgatctcatc taaaacagat gcaaataaat gagcattgtc tggcaaggta
      241 ttgacgatcg tcaaaaagtg gactcgttcg actttcaata caatgtcttc aatcatttga
      301 accagccgat cctcttcctc agtcctagtc tcaggagttc ctttttcgcg aaacaatacg
      361 aagcgggaaa cgccattttc atcaggtgcc ttcacgattt ttagatcacg gatatctcga
      421 gaaatcgttg cctgcgtggc ggtaacgcca taatgcccaa gtttgtttaa tagttcttct
      481 tgcgtacgga tcgggaattc ttcaatgact tgtttaatca gcaaatgtct ttgagctttg
      541 cgcattttct ttctccttta cgaaagattt cacatgtatc ataacaaaaa aaagaaggat
      601 tgcccaataa tattcgtctt ttatacctaa aattatttta tggaaaaagg ataataaaga
      661 aacaaagaga aatctactgt ttttcttgaa ttctttttat ctttcaggta aactattgaa
      721 tgttgagcag aaaagtatat ttgctcttgc gatacggatc ttttgtctgt ataatagctg
      781 agggtgcttt atgcatcagc aaatccacat tcttattgga ttgtcggtag gtgcttgaaa
      841 aagtttgccg gcaaggtaga agataagaag aggaaaaata aaaccaaatc ggaggaaaca
      901 aacatggcag taatttctat gaaacaatta ctagaagccg gcgtacactt tggtcaccaa
      961 actcgtcgct ggaatccaaa aatgaagaaa tatatcttca cagaaagaaa cggaatctac
     1021 atcattgact tgcaaaaaac agttaaatta gtagatgcag cttatgatta catgaagagc
     1081 gttgctgaag aaggcggcgt agcattattc gtaggtacaa aaaaacaagc acaagaagca
     1141 atcaaagaag aagcaactcg tgcaggacaa tacttcgtta accatcgttg gttaggcgga
     1201 acattgacaa actgggatac tatccaaaaa cgtatcagcc gtttgaaaca aatcaatgca
     1261 atggaagaag atggaacttt tgaagttcta cctaaaaaag aagttgctgg tttgaacaaa
     1321 gaacgtgaac gtcttgaaaa attcttaggc ggtatcgctg atatgccaag aatcccagat
     1381 gtaatgtaca tcgttgaccc acgtaaagaa cgtattgctg ttcaagaagc acacaaatta
     1441 aatatcccaa tcgtagctat ggttgataca aactgcgatc cagatgaaat cgacgtagta
     1501 atcccttcaa acgacgatgc aattcgtgcc gttaaattga ttacttcaaa aatggctgat
     1561 gctttcatcg aaggaaacca aggtgaagat caagtagttg aagaagattt cacagaagaa
     1621 aacacagcaa catcaatcga agaaatcgta gatgttgttg aaggcgacaa ttcttcagca
     1681 gaataatttt gaatgatgga gctgtttcaa aggctgggcg acaagcctca ctctcctttg
     1741 aagcagcttt ttttaaaaag aaaaacacac aggaggagtt aaacatggca gacgttacag
     1801 ctaaaatggt aaaagaacta cgcgaaatga ctggcgtagg aatgatggat gcgaaaaaag
     1861 cattagttga agtagaaggc gacatggaaa aagcagttga tcttttacgt gaaaaaggaa
     1921 tggcaaaggc tgcgaagaaa aatgaccgca ttgcagctga aggattggct tcagttgctg
     1981 tgaaaggtaa cactgcagca atcgttgaag taaactcaga aacagacttt gtttctaaaa
     2041 acgaaatgtt ccaagattta gtaaaagaaa ttgcagaact tgttgctgaa aacaaaccag
     2101 cagacatgga agctgcaatg aaaatcaaaa cagataaagg tacaattgaa tctgacttga
     2161 tcgaagcaac tcaagttatc ggagaaaaaa tcagcttccg tcgctttgaa gttgtagaaa
     2221 aagacgacaa tgctgcattt ggtggatact tgcatatggg cggacgtatt gccgtattga
     2281 ctgttctaga tggaacaact gacgaaacag ttgcaagaga cgtagctatg cacgtagcag
     2341 ctatcaatcc tcgttatgta aacgaatcac aaattccaga agctgaatta gaacatgaaa
     2401 aaactgttct tacagaacaa gcattgaacg aaggcaaacc agcgaatatc gttgaaaaaa
     2461 tggtagaagg acgcttgaag aaattcaaag cagaaatcgc tttggtggac caaccattcg
     2521 ttaaagatcc tgatatgact gttgaaaaat acgttgcatc aaaaggcgca actgttaaaa
     2581 cattcgtacg tttcgaagtt ggcgaaggta tcgaaaaacg cgaagataac tttgtagaag
     2641 aagttatgag ccaagtgaaa aaataattct gttgaataac agacgggaac gcacggtttg
     2701 tgcgctccct ttttttaagc aaaacaagaa aaaccagtct tagagtgtcg aaatcatcta
     2761 agtatggtaa aatggcaata gaaaatattg gaggaatgaa catggtcaca ccgaaatatc
     2821 agcgtgtagt cttaaaattg agtggagaag ctttagctgg ggacgcagga tttggaatca
     2881 aaccaccagt aatcaaagaa atcatccaag aaatcaaaga agttcacgag ctgggcgttg
     2941 aaatggcaat cgttgtgggc ggcggaaata tctggcgcgg acaaatcgga gcacaaatgg
     3001 gcatggaacg tgctcaagct gattacatgg gtatgttagc tacagttatg aatgccttag
     3061 ctttacagga tacgttggaa aacgtgggtg taccgacacg cgtgcaaact tcgattgaaa
     3121 tgcgtcaaat cgcagagcca tatattcgcc gtaaagcaga acgtcattta gaaaaaggac
     3181 ggatcgttat ctttgcaggc ggtaccggca acccgtactt ctcgacagac acaacagcag
     3241 cattacgcgc ggcagaaatc ggcgcagatg ttatcctgat ggctaaaaac aatgtagacg
     3301 gtgtttattc tgctgatccg aaagtagatg caaatgcagt aaaatttgaa gaactgactc
     3361 atctggaagt tattgccaaa ggattacaag tcatggattc aactgcaagt tcactaagca
     3421 tggataatga tattccactg cttgtcttca atttaaatga acacggaaat attcgccgtg
     3481 caatcttagg cgaaaatatc ggaacaactg taagggggaa ataaaaatgg cagatgcaat
     3541 tatgacagaa gcaaaagaaa aaatgaaaaa agcagcacaa aatcttcaac gtgaacttgg
     3601 gcaaatccgc gcaggccgtg cgaacgctag ccttttggac cgtattactg tgaactacta
     3661 cggtgcgcca acaccattga accaaatggc atcaatccaa attccagaag cacgtgtttt
     3721 aatgatcaca ccttttgaca agagcattct tcaagatgtt gaaaaagcta ttatggcaag
     3781 tgatatcgga atcagtccaa ccaatgatgg aaatgtcatt cgcttagtta tcccgcaact
     3841 gacagaagaa cgcagaaaag aattagctaa agatgttaaa aaagaagcag aaaatgcaaa
     3901 aatcgcgatt cgtaacatcc gtcgtgatgc gatcgatgaa tataaaaaac aacaaaaaaa
     3961 tggtgatatt accgaagatg atttgcgcgg attagaaaaa gacgtacaaa cattgacaga
     4021 taacagcatc aaagaagtag ataagatcgc tgctgataaa gaaaaagaat tattggaagt
     4081 ttaataaaat cataaaaaca gaggggcgga tcattccgct tcctctgttt ttatttatat
     4141 ttcttttatt taaaaattaa aaaaagaaaa aattattcag tttttaaata aaaaaacaat
     4201 gatt
//
LOCUS       DANKHL010000216         4150 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000216 DANKHL010000000
VERSION     DANKHL010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.226"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            133..636
                     /locus_tag="PW101_002533"
     CDS             133..636
                     /locus_tag="PW101_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL2514904.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            763..1800
                     /locus_tag="PW101_002534"
     CDS             763..1800
                     /locus_tag="PW101_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514905.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            1815..3512
                     /locus_tag="PW101_002535"
     CDS             1815..3512
                     /locus_tag="PW101_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HDL2514906.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKIPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            3505..3966
                     /locus_tag="PW101_002536"
     CDS             3505..3966
                     /locus_tag="PW101_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HDL2514907.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            3979..>4150
                     /locus_tag="PW101_002537"
     CDS             3979..>4150
                     /locus_tag="PW101_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514908.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIE"
BASE COUNT         1451 a          523 c          858 g         1318 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcctactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatgcgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cctgtcgatt ttgatgaagt gaaagaacag
      241 attggattaa atgacgaata tgaagagtac gcgattcatg attatgaact gccttttgag
      301 attgatgagt atacgccaat tgaagagatt aatcggttgt gtaacttagc ggaggaacta
      361 gaaggaacgc caattggtga agctgcttct gagattcaac acgctttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatt gtctattatc cagattgcga tgatatgagt
      481 gatgtggcgc attacttgat tgtagaagct ggtgattttg gcgaagttcc agaacaattg
      541 aagccgtata ttgattttga tgcttgcggt cgagatttgg agattggagg aaattatttg
      601 gttactagtc gtggagtttt tgagtatcca gaatgaatca aacactatat atagtgtttg
      661 attagtatat gtaagcaaaa aaacacaata tatagagtgc aaactgaagg gtaatgtgat
      721 ataatatttg ccgaagaaat atttttgaaa tgaggtaata ttatggcaaa tattcaaaca
      781 agttttattg atttccataa ttcaattaga ttggacgtag aagacaatac gctacttaag
      841 gactataaag accaagtaat tgatggatta aaagattact tacctgatga tgtgaagttt
      901 gaaacttttt tacagggaag ctattctgtt tataccggaa taaaaagctg tgatgaaaaa
      961 attgattttg acatcgatat tgcagttgct ttcgaaattg atcatactgt ttatgaagat
     1021 ccaagggagc ctaaactgtg ggtaaaagag gcgctagttg aaatttttcc taatgctcaa
     1081 gttaatttaa aagtaccttg tgtaactgct acatttactg gaaaaaagac aaaaaaaaat
     1141 gtgcacgttg atgttgcggt ttatgctaaa gaagatgaaa attatttctt agcaaaagca
     1201 aaagaattca gtgctcccga aaaccgatgc tgggaggaag cggatcccaa agtattaaaa
     1261 gagaaaatta atagtcatgt agcagattca gatgatcgaa aacaatttcg aagatgtatt
     1321 cgctatttaa aaagatggaa agataacaat tttaaccaag aatataaacc aacaggaatt
     1381 ggacttacaa ttaatgtgat ggatacgttc ctagttaata agtccactga ttttttaact
     1441 agaaaagttc agtataatga tatggaatgt atgaagcaga ttgtttcttc gctgaaagat
     1501 tcatttgtgt atgagtacag tgaaacagat ggttggcatt atcgtctaca tgcatatctt
     1561 ccagtgaagc ctaacagtga cacctattca aaaatgacag tgaatcagat gagtgatttt
     1621 aaaaacaaac tttctaaatt atatgatgat ttgattttcg ctatcgatac tgaagatgag
     1681 tatgaagcta caaaacgatt aaataatcaa tttggggagg attttttaat tatttcagaa
     1741 gaagaagtta cagaaaaaaa cttaagaaat gcatttgtta cagactatcc gagtgcttag
     1801 gttggtgtta aagattggat tatactgata ttttaaaaga aaagctaaca gatgttgtgc
     1861 gtttattcga tggtaattac tatggggatt tttcatttgc tacaattgga aagttgcgtt
     1921 taaaaattga aataccaaaa cattttccat tggtacttcc taggatttac attcataatt
     1981 ttgatcattt aggactttac ctaccacatg tagaaaaaga tgggttggta tgtttttctt
     2041 cagatataaa ttatttagta gatgaagaaa attcagaatt ggttttattg cagtgtgtta
     2101 ataaaactat aaaaacaatt gaggaaggtt tgaaagggtt aaataaaaat gattttcgaa
     2161 aagagtttgt tgctttttgg gacaaacaag agaatcaaat ttcgttattc tacctttgtg
     2221 aaccagcgtt gtttatcaag tcagttgata caataatgta cggtaagtct ttggttgctt
     2281 tggataaaga gaacgaatta caattaaatg cagcgagtag attgtttaat ggagagaaag
     2341 aagatactca tgaattagat gcatactatg ttccaattag aaacaagaat aatattatgc
     2401 ccccgaagaa taataaagca tggtcaaaga aggatatttt acaaatagta aaaaaaaatg
     2461 taacaggtgc aaataaaaga ttttttttaa aacttgaaaa aagaaagaat agacataatg
     2521 ttttacttat tctagatatc ccaattgaag aaaagtcaaa taatgtcata attggatttc
     2581 tttttacaaa acaatctgga tctataaaag attataaggg tacgcctagg actgtattag
     2641 ttcagcgtca tgatgaatta tatttggtga atagaacgag cggagaaaac tatggattgg
     2701 gtgctaatat agctattgtt ggggtagggt cagttggcag taaaattgct gaaaatcttg
     2761 gtccattagg tgtgagaaag atgacactca tagacaatga ttgttttcaa gctgaaaatt
     2821 tgtatcgaaa ttcacttggt gcagatagtt taaaaaatgc ccgatctcag aataattttt
     2881 ttaaagttga tttaatgcag gaggagctcg aaaaaaaaat cccatacctt tgtgttgaag
     2941 tggagtcaat gaacgtttta gacttaatta gtatgcaacc tggttttttc aaggaatatg
     3001 actatgtttt tgtttgcata ggagatacaa tggcaagcct ggctttaaat aattattttt
     3061 ccaaaagtag tcaaaaagtt ttctattctt ggctggagcc atatggcatt gggggacaca
     3121 ctctatttat aaattattct ctaaatcaaa aaggttgttt caattgtctt tatacagatt
     3181 caaactctaa aaataggtgt tcaaatagag cttctctggt aaaagaagga caaatttttg
     3241 aaaggacttt agcgagttgc cgctcaaaat ttataccata taatgctatt gcctcgacgg
     3301 aatgtgcaat taatgcaata aaacttttcc ataaagtcaa tcaaaataat atgaccaata
     3361 gtactctttt ttcatggata ggagatggga gtacttttga gaatttagga tatcaatata
     3421 gcgacagaaa tacttttctt aatggagtca ataagtttga agaaagattc ttttctaaat
     3481 attgcaaagt atgtggggag ttagatgatt aaattttcgt gtgaccgtat tgtcattgaa
     3541 gtaagggatg aagttcttaa gatatttgaa caatatcggc agggaaagga tgacttgcct
     3601 gaagctggag gagttcttca tggaaaaaga cttaagaatg ggagcataat aattaccgat
     3661 ataacaacac ctcaaatagg agatatacaa aaaagattac tctttaaaaa aaaccgagaa
     3721 attcatcaac agctttctaa tgagaaatgg atagaatcaa ataagttttc tattgcattg
     3781 ggagaatggc atactcatcc tgaagctgta ccaactccat caaatgttga taagaaaagc
     3841 tggagactga acgtgagcaa gcaacatgat gatagagttt atgtttttat tatcgttgga
     3901 ttttctgatt tgagaatgtg gttactgtca aaaaaatcag atattaggca ggctataaaa
     3961 ctataaagga gtgattaggt gaaatcacaa aagaatattt ggtggtgggg gtttttagta
     4021 ggaaaggtaa tgataccagc ccttctctta atttctctac cgatgattac ttttttcttt
     4081 accaataaaa aaatgagtaa agaagccgtg atttttttct ttggagacca aaaagctacc
     4141 tttattgaaa
//
LOCUS       DANKHL010000217         4143 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000217 DANKHL010000000
VERSION     DANKHL010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.227"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(136..1284)
                     /locus_tag="PW101_002538"
     CDS             complement(136..1284)
                     /locus_tag="PW101_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2514909.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1301..1705)
                     /gene="tnpA"
                     /locus_tag="PW101_002539"
     CDS             complement(1301..1705)
                     /gene="tnpA"
                     /locus_tag="PW101_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HDL2514910.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            2064..2318
                     /locus_tag="PW101_002540"
     CDS             2064..2318
                     /locus_tag="PW101_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2514911.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            2319..2636
                     /locus_tag="PW101_002541"
     CDS             2319..2636
                     /locus_tag="PW101_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2514912.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            3195..4130
                     /locus_tag="PW101_002542"
     CDS             3195..4130
                     /locus_tag="PW101_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514913.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT         1376 a          641 c          731 g         1395 t
ORIGIN      
        1 cctgacggtt cttaacgctt gaagtgggag cttcttggga atagagcata cttgatagcg
       61 tatttcttta ctaacagcgg tgtctcttat ttaccaagct atccccgtag ttcctacggt
      121 tcttgatgat tggatctaag ccaatcccat agaatgtagt cttagacctt cggtcagaat
      181 attgatacta gcattgatat cacgatcatg atgagtatga caaacaggac aagtccattc
      241 tcgaatttca agagatttct tgccatcttt atgtccgcac tctgaacaaa tctgactaga
      301 cggaaaccat ttatctactt tgatgatttt tcgtccatac cagtcagcct tgtattgtaa
      361 tttagtcaca aactttgacc aagaaacatc agaaatactt ttagctaatt tatgatttcg
      421 caacatacct tttgtgttta agtcttcaat acagataata tcgtgatttt tgatcatttc
      481 tgtactcaac ttattcagaa aatcagtacg ttgattcatt actttttcat gcaatctagc
      541 tactttgcgt ttttgttttt gataattttt agcttcaaat agattggtac ctttattttt
      601 agccaataaa gcacgcctag acaatttgcg ttgttcacgc tttagtttct tttccatttt
      661 ggacgtgaat ttattattat caattttttg tccatcagaa agaatcgcaa agtccataat
      721 acctaagtca ataccaattg tggaattagt tttaggtaat tcgataattt cttctttaca
      781 caacaaagaa atatagtatt taccgctaga atgacgtgat attgtagcag atttgataat
      841 cccttttggt tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat
      901 gaatttacta tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac
      961 aggatttttc ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt
     1021 gtatgcttta tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa
     1081 aaacggaaat tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc
     1141 atttttaact tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg
     1201 gacacaacca aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa
     1261 tttgtatgct tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt
     1321 gattttggat atacttctta atcacatcaa ttggtgcgcc accagttgtc agaagacaaa
     1381 aacttttaga ccagaacatt tctttccaaa gaaattgttt gactcttgga aagtcacgtt
     1441 ttatcaatct tgagctggca cttttatagg cattgatgaa ttttgtcaat tctgttttag
     1501 gttgagcttt gaacataata tgaacgtggt ctttatcatg attccactcc actaaagtaa
     1561 tgtgatacga ttctgaaatt ctttcaaaag tagttttggc ataatcagat atttcatcat
     1621 caatcacttg tctacgatat ttcgttacta aaacaaggtg gtaataaaga agaaatactg
     1681 aatgattatt tgtatctaat tccatgttat atcaactcat ttcttgtata gactgattgt
     1741 aacatggaac aaaataaaaa ggcaatacgc cttagttgtc ggtcttcgag ttaccaacgg
     1801 taaccctcat accgaacggc attcatcacc cacctataga ggatgggcga cttctgccta
     1861 attctgttaa atggatgtta acggaagtgt atggtgagta gttcactcgt attttgatat
     1921 cgtttgtata tgttttcagc gttctaagtg gcttattttg tccaataata atatggagtg
     1981 gaatccctta agaaacaccg ttggcaattg ttgttataac atgttataat ttattcatca
     2041 aaaactaaaa ggagtgtgat aacatgggaa aagaactaaa ggttagaaag atcggcaata
     2101 gcgtgggagt gatccttcca agctctcttg gattaaaaag cggagacatt atccaagcca
     2161 aacaagaagg caacttattc atattagata ctactcaaat agcaaaagaa cacgatcgca
     2221 aattgattga agaaagtttc caagactttg agaaaggttt aacagtttca gaaattgaaa
     2281 tggtaaaagc tttcggaaaa tacggctgga gtgaatagat gaaagagtac acgctagttt
     2341 attctaaaag ttttcagacc agccttgaaa cgataataga agaatgggaa aatgagcttg
     2401 ctctttctcc agccagcata aaaagatttg taggtgcgat ccagaaggct ttagaactaa
     2461 ccaaaaattt tccaactatg catgaggaag tttccgaaat ctatggaatg gatatcccaa
     2521 catatcggat cgtgatcggc aaaagctatg ccttatttta tcggatcgac catgaaaaaa
     2581 aagagatact tgtaggacgt attttccaat ctaaacaaat gaaactaggc ttttaacaat
     2641 aattaggaaa aagtcgctca tcctctacag gtgggtgatt aatgccgctc gatatggttg
     2701 ttaaccgtcg gttactcgaa gattgatata taagacttag taccttttta ttttgctcca
     2761 tgttatggcc gaaggtctaa agatacaatc attagtctag agaaaaatta agaaccgtag
     2821 gaactacggg gatcgcttgg tgaataagag acactgctat tagtgaaaaa ataagatatc
     2881 aagtacgctc tattcccaaa aatttcgtga ctttactcat gagaggttca agtagtatat
     2941 ttttaatcaa aattgacgta acttttaata aatactacct aaataaggta gtatttattt
     3001 ttgtgtttta aaagaggaag tccaatatat ctatgggaaa cttcttaaat attatgttgg
     3061 tacatgaaaa tatacatccc tgctagacta tttacaacat tttactttgt acgtattctt
     3121 gttttaaaag gaatgggata aaatataggt acaataacta tttgatgcta aatataatta
     3181 atggaggaga gatagtgaac atacttaatg aaaaaagata tgtatggttt gctggtaaaa
     3241 aatatattac tactttcaaa agattatccg agttaatgca gcagactgtt aaaagtgcta
     3301 tgcctgaagg atacaagttt aattttgttc aatcttcagt atctactgaa agtatctatt
     3361 tttcaattct ttctgagagt aggctttttc attttagaat aagttctcat gtaaagaaaa
     3421 ctgatgttga tatatatacg ttccgtacaa ataattataa aaatttaaat gatttgagta
     3481 gaagtatcag tgaatatctc aaggggaata gctatctcaa gattcgattt tcacatatat
     3541 taattttact accatatttg aatgagaatt tcaccctaat tcaagtagat aagtctttca
     3601 ttgcctcagg taatcaagga aatatgaata ttacaagtaa caaaacgtca cgtgtgctat
     3661 tggcgttgat gggttacgga attctttata gagatgacca aaatggccag attctaataa
     3721 atcgggctgg gcgttcatta ttaatgaaat atagaaaatt tataccggaa gattttcaaa
     3781 acttacagtt agaaaacatg cgtttagaag atttatttag attagcagaa gatgataagt
     3841 atagatttga agttttgaaa attcaaaaga atgaactatt gaataagtta tatcctgaga
     3901 atgcgataaa agcacatttt ggtgatgcta atcatatgta tagttggttc attcctaaaa
     3961 gatttagagg gaaaatcaat gctggagatc aagtgaaagt actcaatcga aatagtacag
     4021 atgtcgtaat gataacagat atctatgtag aagatgaaga tataatcaaa tatatgaagc
     4081 ctgtgataaa acttgttaat aaaaataaag gaacccgtgg tgctagttaa ttttttccta
     4141 gag
//
LOCUS       DANKHL010000218         4110 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000218 DANKHL010000000
VERSION     DANKHL010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.229"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            40..1464
                     /locus_tag="PW101_002543"
     CDS             40..1464
                     /locus_tag="PW101_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2514914.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(1645..2418)
                     /locus_tag="PW101_002544"
     CDS             complement(1645..2418)
                     /locus_tag="PW101_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2514915.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2415..3320)
                     /locus_tag="PW101_002545"
     CDS             complement(2415..3320)
                     /locus_tag="PW101_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2514916.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(3474..3794)
                     /locus_tag="PW101_002546"
     CDS             complement(3474..3794)
                     /locus_tag="PW101_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDL2514917.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         1238 a          758 c          820 g         1294 t
ORIGIN      
        1 ctttttgata aaagcggttt tattttggag gatgcttaca tggatacaga gcaacagaaa
       61 aaaggaattg gtttaattcc acttgctgcc ttagtaattg gttcagcgat tggaggtggc
      121 gtatttggta ttatgacgga catctccgga tcggctggcg gcccagctat tttcagttgg
      181 gtactcgtag gaacagcaat gttgatgctc tccttgtcga tcaataatat caataaaaaa
      241 cgtccagatt taaaaggcgg gatctttagt tacgcagaag aaggttttgg ccgtttcgct
      301 ggttttatca gcggttgggg ttactggctc acatgctggt taggcaacgt agcttttgca
      361 acattgttga tgagcgcatt aggatatttc ttcccagtat ttgaaggtgg gcaaaatctg
      421 gcatcgatcg tgatgagtac agtcttttta tggggatatg catggctagt gaatcgggga
      481 gtcgaaaatg ctagtttcgt caatgctatc gtcacattct gtaaattagt gccattattt
      541 ttatttattg tagtagctat cagcacgttt tcgatccaac aattcattga taacttttct
      601 agcgacatta tattgaatgc gaatggacag ccgatcccgt tttccagtca agtattgagt
      661 tgcatcatgg tgatgctttg ggtatttgtt ggtattgaag gagcctctgt cgtaggttca
      721 cgagcaaaaa aacaatcaga tgttgggaaa gcaacgattc tagggatatt cggcttgatt
      781 attatttacg tcttggtttc catgcttcca tatgggacaa tgacgatgaa tcagctgcaa
      841 acgatcaagg cgcagcctgc tatgggctat gtgtttgaaa tgatggttgg taaatgggga
      901 gcagtcgtga tcaacggcgg tttgatcatt tctttagttg gtgtttggct ctcatggacg
      961 atcttaccga ttgaaacaat gggaaacatg gctgaagacg gacttttgcc taaagcatgg
     1021 ggaaaactca acaaaaatgg ggcaccaact tatgctatcg tattgacgac gttatgtacg
     1081 aatgtctttt tactttcatt attattcact gacagtgctt ataactttgc ttacacactt
     1141 ggtacagcag ctattttctt cacgtggctt ttcttaggat tgtaccaaat gaaactttct
     1201 tatcagcgga aagaatgggt ccaattttta gtagggcttt tagcaagtgc ctttcaagta
     1261 tgggctatgc tagtcgtagc ctttaatgaa gtaatgcttg ttcttgttct tttcttgcca
     1321 ggcatttatt tttacctcaa agcacgtaag gagcaaaacg agcctgtaca acatatccac
     1381 ttagataaga ttttacttat aggtgtgacg atggtcggag tgattgcaat ggttttattt
     1441 gccactggga tgcttcacgt atagaagtat aaaagaagta taaagagagg ttgtgagcct
     1501 gtcgtttatt caaaaacata ctaggaaagg agctgtgaca caaatgttgt cacagctcct
     1561 ttttcagaat aaacggtgtt taagagacag ccaatcggca ataaggctga acgtctcttt
     1621 cacagcctct tttttgatga gtcatcatac attttttcgc ttaaatacga gatatcctaa
     1681 tgctaagaac aaaacgatgt aagctaaatt accgaatagc atttgattag tagaaagatg
     1741 agtcaattgc ttcatgatct cttcgtttcc aatttggttt tgcagattca acatactgat
     1801 cggattccat ttgatccact cccatttttg gatgagtgca ataaggatac cagatatgat
     1861 tgacgaagca aaataaaaga cgattcctgc tgagatggat gcgccggaac tattgataaa
     1921 gcaagcaatc atcaacacta aacttaaaat cagccataaa ccgatataac tacccaaagt
     1981 ataagtgaac aagctttgga tcagtgtttg accagtagaa accttctccg tgaaggaaat
     2041 cgaagggaaa agaatcagtt tcatcaacac agtgacaata atagctaaaa ggtataaata
     2101 aacagaataa ataactaatg tgatccattt gcttactaat atttctcctc gagaatactt
     2161 tcgataaagg agatttttca atgtaccata ttgtgcttcc atcgaaatga tcgtgcttgc
     2221 agctgcaatc atgataaaga caatccaaga tgtggcacta aaaagctgag cagtcatttc
     2281 aactggatca atgatcgtcg tgtatttttt ggctagaagt gctgttccta tgagcaaaac
     2341 aactaataga acagataaaa cagcagttga ctttttcttg atcaatttaa ataattcttg
     2401 ttttacaagc aatgtcattt ttcttcttcc ccctttactg aatccagaat ttccaataga
     2461 gaagattcca aatcattcgt cagatgctgg acgtctgtca catggatatt ttgtgcagac
     2521 aatagctgga tcacggttcc taataattct tcgttttctt ttaaaagctt gattttatca
     2581 ggatcttcca aagtatatcc agcttctgtc aagatcgttc gagcagctag atcatttgtt
     2641 gtagagataa cgatgaaatc ttgcgctgtt tggttcaatt cagccattgt acattgtttg
     2701 atgatctttc cttgattgat caggatcagc tcatcgacca ttttttcaag ctcgctgagt
     2761 aagtgactgg agatcaagaa agaggtgccc tgagtagcca agtcggtaat gatttttcgc
     2821 acatctcttg ttgcttgtgg atccaaaccg ttcatcggct catctaaaat aacgatagac
     2881 ggatggttca gtaaggcaag agcaattccc aatttttgtt tcattcccaa cgagtaggat
     2941 ttcgcttttt tgtgaatgta cttttccatc ttgagctgat tcacgatagt gtcgatagca
     3001 gatacatcgt tggtttcaga gaataacttc agatgatcat agcctgttaa gaatggatag
     3061 atacccgggt attcgatcaa tgctcctact tgttccaacc ctttatgact agagggagaa
     3121 atctcatttt caccaataca gattgttcca tctgagtaat ggatcagtcc taaaatagct
     3181 ttcatgatcg ttgtttttcc agcgccatta ggacctacta atccaacgat tttgccaggg
     3241 gataagctaa aactgacatc gctgagggct tgtttgtgac caaagttctt ggataattct
     3301 tggaccgaaa gacttttcaa aaatattcac tccttttcta tagatagtac aagcatacac
     3361 ctgatgagtg gagaagtcgc taggtcattg gacttatttc ttattttttc ataaaaaaat
     3421 tgaatattaa ataaaaaggc ttattttttc tgatcagtac ttattccaag tgtctagtgg
     3481 ccgctagtaa tcttgatgcc aatgatactg atgagtagca tccccacaaa gaaccaagtg
     3541 gaaagattca gcttatcatg aaacaagacc acaccaatta tgaccgaacc aactgcgcca
     3601 atccctgtcc agataggata agagagactc atcggcaaac ttttgattgc ctttgacaaa
     3661 aagtaaaaac tagcaatcat gccaatcact gtgtaaaaag aataattgat tttagaaaaa
     3721 ccatcactcc atttcatcat cgttgaccag aatacttcca aaattcctgc tatgattaat
     3781 tccatccatg ccatacttac attcctcctt aactataaaa aaatacacaa aaagaggaaa
     3841 atcatccctt caaagaccta atggaatgat catcctcttt atcctacccg gtggcagatt
     3901 gtatatcttt ttctgtagta tagcacacct atccagatat cggcaatctc atacttttta
     3961 gtaaagtgta ccaagcactg acggatatat cgttagcgga aaagaagatg aatgctttgc
     4021 aatttcatag aaagaagttg ttatttatta atcatttaaa aaaaacagta actagacctt
     4081 atttctatat attatcgggc aataaattaa
//
LOCUS       DANKHL010000219         4091 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000219 DANKHL010000000
VERSION     DANKHL010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.230"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..756
                     /locus_tag="PW101_002547"
     CDS             <1..756
                     /locus_tag="PW101_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HDL2514918.1"
                     /translation="IEYTGNLSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNG
                     DIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRD
                     ALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRIT
                     VKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGF
                     SDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            901..1206
                     /locus_tag="PW101_002548"
     CDS             901..1206
                     /locus_tag="PW101_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514919.1"
                     /translation="MLNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDA
                     KISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1823..>4091
                     /gene="esp"
                     /locus_tag="PW101_002549"
     CDS             1823..>4091
                     /gene="esp"
                     /locus_tag="PW101_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HDL2514920.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
BASE COUNT         1505 a          557 c          772 g         1257 t
ORIGIN      
        1 attgaatata caggaaatct aagtaaaact atcgagcaaa tcaaaataga taatctgtta
       61 tcacaaaatt acgatccaga aatatatctg tttctttatg aaaataaaat actggaatat
      121 gttaaaaatg gagatattcg aaatttagaa aacatggttt ttaatctgag taatggaata
      181 attccaagtg ttagtggaga tacgattcgg tctgaaaaaa attattctat cattgttttt
      241 gaaaagttag cacaaacgtc tataacatta ggaatggata taatagaagc gtatcaaagt
      301 agagatgctt tgatacaaga aaacgaatta gctgtatcgt tacctgaggt cttaaaagtg
      361 agggattctg ggattgttta ttatactaag gaaatcggaa aaactaagat agagcatctt
      421 tctcccttaa tttcttctgt cgttcaattc atcggcttaa atatctataa aagaattact
      481 gtaaaggaaa tagctaatta tttttctgta agtgaaacta aattaagaga aagctttaag
      541 aatgaaatgc atattacgat ttataattat atctctaagc gaaaaatttc tacggctaaa
      601 ataatgttga aatcaaacca tactatcagt gaagtttctt taggtttagg attttcagat
      661 tcttcacact tttcgagagt attcaaaaag tacgcaggtg tatctcctaa gcaatatcaa
      721 ttaggtttag tggataattt caacaacatt agctgagagg ttttatttta ttatgagact
      781 atatcattta cgtagtttgg aggagttgag gaaattggat tttaacaaaa aattttttgg
      841 gaaatttctg atttgagttg tttcgtagaa tctgagcgtc taagtaatgg taataatagt
      901 gtgttaaatg aatatgacaa agtagtaatt aataaagtaa aaagtttatg tgaagaagct
      961 agagaatcta tcagtcatat taatggtagc aattttaagc aaacattatt taatctgatt
     1021 aagctagatg ctaaaatatc aagttattta ttctttttaa tacatgatga atttatggat
     1081 tatcaaaaat tggatcagtt aattgaaaga gaaagctggg aagattatta cgtgggtcta
     1141 actttttcag aaaagttaaa caactacaag ttaatagatt ataagtattt gagtgaaagc
     1201 aattaattat tcatgtagag tactagacac tttaaatatg gacaattcaa ttataaagaa
     1261 aaagaatcaa taatttaatg aggctgtgac aaatgtcatt ttttgtttcg ctaaaaacga
     1321 gtgaaaaaat gttgataact ccaatcattc gtttaagatt ctaggaatca ttcaggaatg
     1381 ctgtgaaacc gatactttat aattataaag aaggtgattt caaattccga agaaggaatt
     1441 tgaagtcacc tttttactta tgtcacagtc tcattcagta atgcgggata aggctcctta
     1501 ttccgctatt tgaatactct gaagtttaat ctaagaaaga gtaatatttt gaaaacatgc
     1561 ttcatcgcat agaaatgaac ttaatttcag aaagaataca tacatatctt tttatatgtt
     1621 ttcagctgct gaatggttaa aaagcacaaa tatccgaaaa aaacatgaag aaatgtactt
     1681 tttttgttgt aatgtgcatg aaattcttta agtaaactag ttaacataag tttagtaaga
     1741 ttcctattct gtgaataata cgtatcagat ttatctttta ttcttactaa gaaaataact
     1801 attaagggag ttgatttgaa taatggttag caagaataat aagagagtat ttctagaaaa
     1861 aacaaagaaa cgagtattga agtacagtat caaaaaatta agtgttgggg ttgcatctgt
     1921 tttggttggt gttggtcttg tcttgggaac aactgaacta gttcaagcac aagatgaaat
     1981 ttctccaagc accccacttg aaacagcgat aagctccgta caagtaggtg acaaagtagc
     2041 ttcggggaat acttttcagg agaatcctgg ttatacgaaa aattataact tttcggattt
     2101 acaatttagt cctcaagaac taactggaga cacactaaaa ggcaatacaa ttggatttga
     2161 agtttacgga aaacataata ttgcagcatc aactaaaaac tgggaaatcc gtcttcaatt
     2221 agatgaacga ttagctaaat atgtagaaaa aattcaggtc gatccaaaga aaggaattgg
     2281 atcttctaga cggacctttg taagaattaa tgattcgctg ggtagaccta caaatatttg
     2341 gaaggtcaat tatattcgtg caagtgatgg attatttgct ggggctgaaa caactgatac
     2401 acaaactgct cctaatgggg taattacgtt tgaaaagagc ttagatgaaa tttttaaaga
     2461 gataggcata gacaatctca agaccgatcg tttaatgtat cgaatctact tggtaagtca
     2521 tcaagacgat gataaaattg taccaggtat tgatagtacg ggctactttt taacagattc
     2581 agatgatttc tataatagct tagatgtatc tgaaaataat cctgaccagt ttaaacatgg
     2641 atcagtaagt gcaaaatatg aggaacctaa tactcaaaca aaagatggtt caggatccac
     2701 aggtgctaat ggagctataa tcttagatca taagttaacg aaaaattata atttctcata
     2761 ttcagcttcc gcaaagggca cgccttggta tgctaattac aaaattgatg aacgattagt
     2821 tccatatgta gcgggtatac agatgcacat ggttcaggct gacaaagtaa catatgatgt
     2881 ttcttttgaa tcagggaaaa aagtggctga tttagcaatt gaacggcgca aagatcatga
     2941 aaattatggt gtaggttcaa ttactgataa tgatcttacc aaacttattg attttgctaa
     3001 tgcaagtcca cgtccagtcg ttattcgata tgttttacaa ttgactaagc cattagatga
     3061 aatcttagaa gatatgaaag caacagcaca agttgaagaa aataaaccat ttggtgaaga
     3121 tttcatcttt gattcttggt tgtcggatac gaataaaaaa ttaatccaga acacttatgg
     3181 aacaggttat tattatttgc aagatattga tggtgatgga aaccctgacg ataaagaaga
     3241 gagcggagac acgaatccat atatcgggaa acctgaatta gaagaagtat atgatgttga
     3301 cacaacagtt aaggggaaag tattcatcca cgagttagcg ggaacaggtc acaaagccca
     3361 acttgttgat aaagaaggta ctgtattagc agaaaaaact atcgctccaa atgaaaaaga
     3421 tggggctcca atttcagata ctgtagaatt tgaatttacg ggtgtagatt caagtaaatt
     3481 aatcgcgaaa gatgaattaa aaatccaaat cgtttctcca ggttttgata aaccagaaga
     3541 aggttcaacc gttattaagg aatcaccaaa agcggttgat aaacaaaccg tggtagttgg
     3601 atttaaacca gatgctaaag aatcaattcg gaataataaa aacttacctg aagatgcaga
     3661 gtattcatgg aaaacagagc ctgatacttc taacgttact gatagtacga aaggtattgt
     3721 aactgttaag atcggaaatc gaactttcga cgtggatgta gagtttgctg taaaagcttc
     3781 tcaagctatg gaaaatgatg caacatacgt acctataaca acaactccag aaacgacagt
     3841 tcaaagtgga aaacctacat ttgataaacc agatgttcct ttagctaaag atgccttttc
     3901 aattttagat gtctataata aggacttcgg taatgcaagt gttgatgcaa atactggtat
     3961 tgttacattc actccagcta aaggtgtagg agaatcggag ccgattactg gaacaattcc
     4021 tattaaaatt gtttaccaag atggttctgt aggcacgacc gatttagcag taactgtaag
     4081 taaagatatt t
//
LOCUS       DANKHL010000220         4069 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000220 DANKHL010000000
VERSION     DANKHL010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.231"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            85..1434
                     /locus_tag="PW101_002550"
     CDS             85..1434
                     /locus_tag="PW101_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2514921.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="PW101_002551"
     CDS             complement(1562..2152)
                     /locus_tag="PW101_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL2514922.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="PW101_002552"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="PW101_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL2514923.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1175 a          744 c          789 g         1361 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
     3721 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
     3781 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
     3841 acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     3901 gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct ccatatactt
     3961 tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt tcttttataa
     4021 tatagatttt agacatatat atgatataaa aatagttttc gcaaaaaaa
//
LOCUS       DANKHL010000221         4025 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000221 DANKHL010000000
VERSION     DANKHL010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.232"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(69..764)
                     /locus_tag="PW101_002553"
     CDS             complement(69..764)
                     /locus_tag="PW101_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2514924.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            918..1196
                     /locus_tag="PW101_002554"
     CDS             918..1196
                     /locus_tag="PW101_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514925.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1215..2423
                     /locus_tag="PW101_002555"
     CDS             1215..2423
                     /locus_tag="PW101_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2514926.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2571..2936)
                     /locus_tag="PW101_002556"
     CDS             complement(2571..2936)
                     /locus_tag="PW101_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HDL2514927.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3083..3856)
                     /locus_tag="PW101_002557"
     CDS             complement(3083..3856)
                     /locus_tag="PW101_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2514928.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
BASE COUNT         1204 a          772 c          676 g         1373 t
ORIGIN      
        1 ggaactattt ttccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc
       61 tttgttcatc aaaagataat attgtcttct aacaagtcca tattcaaaat aacgatatga
      121 tggtctttaa cggtgataat accatctttt ttcagttgtt ttaaaattct gtttacactg
      181 ctagcagttg agatgccaca aaatttggcg atttcttcat tcgtgatcga aaaatcgatc
      241 agcatccctt cttctgtccg cacaccgaat aaatcatata attcgtaaag ttgcgtacaa
      301 acagctccca gctttccgtt taatagcatt tgctgcattt ttttcatcga ataaagcaga
      361 cgatagcggt aatactcttt tacatagtgt tgtagctttg ggtcttggtt gatattttgc
      421 caaaagcgca cacgattgat tcgataaaac tctgcatatt ctgattcaac tcgaatatta
      481 tacggcgaat cgatgaattg tgaatattcg tcttttaaaa gagagacgat ttcaagatct
      541 ttgatatagc gaaggttaaa ttctctccca tcatttgaaa taacacttgc ttttaccgtt
      601 ccctctttca agatatacgt atgggaatct tccatccctt cgaatgtcaa atatttatga
      661 tatcctttgc gtataatcgg aaaatcattt ttatccaaat attcctttaa gactctacta
      721 tccaatgatg ctcccccatc caaactttca tctaaaaaga tcatacagta ctttaaaact
      781 ttttaaagtc caaaggtatt ttactaatga tattaacatt tgttaagtac ctttttccat
      841 cacttatgcg atttttttcc ttttttttat tcgttatact gcagaagtaa aattttttaa
      901 gaatagaggg aaaaaacatg aattactgga tgaacacaat cattaatcga ttagaaacag
      961 catatcaaac aagatttgat atgaaagctt ctttagtctt tttaaatgat gcgtatcaaa
     1021 actcgatcga attgatcaaa gcagtggatg agaatccaac aaacgaatgc gaagaatttt
     1081 taaatctgtt tatgtctaca agagatttat ttattcgcca acttgttgat cgctatccat
     1141 ccaattatca cgatgtagaa gttcaaatac aaaaattaaa agcatattca gcttaagggg
     1201 aatgtataac atacatggaa aagaaattat ttaataaata ttttattgga atcacattga
     1261 tcaattttat tgtctacctg atttattatc tgttgatggt catcatggca gtcattgcgc
     1321 aagacacctt acacgccagc ttgagccaag caggttttgc ttctgggatc tatatcatcg
     1381 gaacattgct ggcacgctta ttcatgggga aacagttaga actatttggt cggaagttta
     1441 ccttaagagg cggcgcgatc ttctatctcc tttcaacatt agcatatctt tatacgccaa
     1501 ctatcggggc attgtattta gttcgattct tgaatggatt cggctacggt acagtttcta
     1561 cagctacaaa tgctatcgtt actgcatata tccctgcatc taaaaaaggg gaaggcatca
     1621 attattatgg attaagtact agtttagctg ccgctgttgg gccattttta ggaatgattt
     1681 tgctgaatat gactgatttt cgtttcatta tctggttttc aattgtactg gtcttctttg
     1741 ttacgattgc ctgccttgtc tttcctgtaa aaaatattcc attatctgaa gaacaaaaaa
     1801 tatctcttcg ctcatggaat atcaatagtt ttattgaaaa gaaagcactt ttcattacag
     1861 gaattgcctt tttgatgggt ctttcttatt caagtgtttt atcattttta tcttcttata
     1921 caaaagtaat ccacttagtt gctgctgggt catttttctt cgtcgtatat gctttggtca
     1981 tcacatttac acgtccattg actggaagga ttttcgatgt caaaggagaa caatacgtga
     2041 tgtatcctag ctatttattc ttaacagcag gattattttt actgagtgta acgacgaata
     2101 gtgtgacatt gcttatctct ggagcattag tcggcctagg ttatggtacg tttatgtcaa
     2161 atggacaggc tgtttgttta aaaatcgtcc gcgaacatcg aatcagtatt gctttatcta
     2221 cctactttat tggtttagac ctaggcttag gcgttggtcc ttatttgatg gggatgctaa
     2281 aatctcttac ttcatttaga ggattgtatg tgattgccgg agtcattcca ttgatatgta
     2341 cagcactcta tcttgttaat ggccgaaaac atagtcatga agcagagggc gaattgattg
     2401 aatcattgaa aaatgaaatt tgatcttgat gaatagataa aaaaaaccgt tcagatctga
     2461 aaataatctg agcggttttt tgagtaaaaa tagtttatga ttattcttaa ttaaacatct
     2521 atttattttt aaagctttca tttatcgata agaatagttt aactgttgat ttattccatt
     2581 ttccgttcat ctggtacgat gccttgactt agcaaccact tgtggaagaa ccattctgcc
     2641 acaccgatca atagagcagg aatcaatact ttcatccaaa tatcgactgt atcattcata
     2701 aaatatagat agagccacaa gataactgca gctgaaccag catctgcgat cgtcgctgcg
     2761 atgttcccta ctttacgtaa gatcaacaga tcccctagca catagatcat gactacaata
     2821 attaccccaa caatcgttga atccataaag ctaacaccgt ataaacctgt cagcacgacc
     2881 cacagaatcg cccagatcat gattgccttc actaccaacg ccttcacatg tttcattttc
     2941 tgcactcctt tctcctggat aaaaaagtaa gattatctaa caagataacc ttttcattat
     3001 tataccatcg gctgagagaa gcgagcaaag ataaggtcct atctaaaaat gaggaagcca
     3061 cttagaacat tcacgtaata atttatccgt acagacattc gatatggtgt tcctttactt
     3121 tcgcatactg ttcttttgtc agttctttac ttgtcaccag ataatcgatt tcatgaaaag
     3181 caatggcttg atgcatgaat ttatgtgcaa ttttttgctg tgtaactaaa acggcaagcc
     3241 ggtctgattg ttttctaaaa gcttttttga ccattgcctc tccttcatga gtttctgtta
     3301 ctccttggct gaaatctagc ccgcgacaac tcattatcgc taaatccgca taatatgaca
     3361 atgcatcgtt ataggctttt gaaccgttga ttgttgctcg tttcggaaca attgcccctc
     3421 ccaatacaga tacttttgcc gttgtatatt cactcaacat agcagctgtg acgatgccat
     3481 tcgtaatgat ctgcaaacca tcaaatcttg ctaggtttct gactaagtgt aaagaggttg
     3541 tactggaatc caagaataag accatatcat ctcgaatcaa agaagaagct gtatctgcaa
     3601 tcttacgttt ttctgtttct tcttctaatt tggtgatctt catcaggtaa ggcacgtcaa
     3661 tttttcgatt gtcaataagc atggcgccac cgcgaactct acggatcaat ccactttttt
     3721 ccagaaaaac aaggtcacgt ttaatcgtgg cttcgctata atttactaac tcgatcaaat
     3781 cggtgactct tgcaaattgg ttgtttttta gataattcac aatctcctgc tgtctattct
     3841 ccacttcatt caacataaaa tcaccccatt tactttatat agtaacagga aaaaagaggt
     3901 tgtgacagaa gtgattggct tcgagaaata agaagaaatt tttcgaaaaa actacttctg
     3961 aacatcgtgt gtggttaaga gggtatgaca agactattgt cataccctct tgaccacaca
     4021 ttttt
//
LOCUS       DANKHL010000222         4016 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000222 DANKHL010000000
VERSION     DANKHL010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.233"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(175..1461)
                     /locus_tag="PW101_002558"
     CDS             complement(175..1461)
                     /locus_tag="PW101_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL2514929.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1454..2722)
                     /locus_tag="PW101_002559"
     CDS             complement(1454..2722)
                     /locus_tag="PW101_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL2514930.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2974..3435
                     /gene="mscL"
                     /locus_tag="PW101_002560"
     CDS             2974..3435
                     /gene="mscL"
                     /locus_tag="PW101_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDL2514931.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3494..3940)
                     /locus_tag="PW101_002561"
     CDS             complement(3494..3940)
                     /locus_tag="PW101_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2514932.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         1136 a          817 c          707 g         1356 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaatac tggtttattt
      181 tggtctcatg taaaactcgc tgaaggcctc ttcacttata aatgcttccc cagctgccag
      241 tacgtctttc atttggatcg aatcgatgat ttcaggaaga tcaaataaag ttcgatcgcc
      301 aaataaactt tgcgtaaatt gattggcaat atattcgatc gaatttaatg attggaaata
      361 ttgtccaagc attttcttct tcagcaaatc gagatttgtt tctgacactt ccggatcatc
      421 agcgaatcca agaatgattt ctttgacttt ttctgctgct ttctctggtt cgtccgtatc
      481 tccgctgaag tccgcaaagt gaaattcacg atccaagctg aattcaaaac caaagctgtc
      541 atcgataatt ccttgattgt acatcgctag atagtttcga gaagtattgc ctaaaatcat
      601 ttggaacaac agattgattg ctgttttata gcgtaacagt tctgttcctt cttgcggcaa
      661 tgtgtctaat cctttaatac caagtacaaa tttatctcgt gtaatggctg cttccaatgc
      721 actttgtttg atgatttctt ttgtgttttc agggaaataa cgtacaattt cttgctttgg
      781 agggaaattt tttgcttctt ggttttcccg aataagtttc atcaatttct caggctccat
      841 cttaccaact acaaataaaa ccatattgct tggctgataa aacgtccgat agcacgtata
      901 caagtcttga gctgtgattt tgtcgatact ttcaaccgtg cccgcaatat cgatatgcaa
      961 tgggtgtgta ggatacaagt tatttagtat gccaaaaaac atccgccagt tagggtcgtc
     1021 ttcatacatt tggatttcct gaccgatgat tcctttttcc ttgtttacag tttcttctgt
     1081 gaagtaaggt gcttgtacaa aatcgatcag agtcgtcaga ttcttttcta cctgatccgt
     1141 cgtagaaaac agatagctgg ttttcgtaaa acttgtgaaa gcattggcgg aagctccttg
     1201 tttcccaaat aattgaaaga catcaccatc ttctttttca aacaacttat gttctaaaaa
     1261 atgggcaatt ccatctggaa ctttcacctt ttctttttca ccatagggaa taaattcgtt
     1321 atcgatcgaa ccataattcg tactgaacaa accatatgtc ttgtgatagt catttttagg
     1381 caacaaatac acggtcaaac cgtttggcag tacttcatga taaagtgttt cattgatttg
     1441 ctcatattct gttttattca tttgcatttc ctcctgctaa aaagaagatt gcctgcaatt
     1501 cgatttgctc agctacttgt tgtatctctt tgatcgttac gctctccata cgtttcaacc
     1561 attcttcatc agataatttt gtttccggca gccagctgtc caaaaaagaa gcttcgattg
     1621 ccgcttgcgg agagtctaga gaaagcagat attggtttct cagcattgct tttgtctgag
     1681 acagttccaa ctctgttatt tcaccatttc gcaaactttc taattgctcg tgaatcagat
     1741 gaagtacttg atttcggttc ttttcatcga taccggtttg gacactcatg aatcctcgga
     1801 acgtatccac gctactagag gcataataag ctagactttc tttctctctg acattcatga
     1861 aaagtttaga gtgaggaaaa ccgccaaata aaccgttaaa gaccattagc gcaaaacgtt
     1921 ctggttcatc gtaatacaca tttgtctgat aagctaggtt cagctttgct tgaacgattg
     1981 gctcacgtac ttgtcgttcc ttcacgatat tcacttgagg ttgggtataa aacagatctg
     2041 gataaactgt ttcccgcgac tcaaatggta attgcccaac tgcttcagct gctttgtccg
     2101 gatcaacatc gccgacaaca aaaatgtcga tttgatcatt tgccatcatt tcttgatagt
     2161 aggacgctag cgaactagca gtgatcttgt ccaaatcttc gactacgccc aaccctggga
     2221 tcttttgttc cgggctgttt tcaaaataaa gttcttgcaa ggcataggaa gcaaaagttt
     2281 gtttatcttc cttcaaactt tccaaatata gacgcagatt gttcttttcc aagtcaaacg
     2341 tcaactgatc aaactgatga tttttaatat ttggataaaa taaaacctct tttaaaaaat
     2401 ctaccgcttg agaaaaaagg tttggatcat tgacatattc accattcaca aaactgatcc
     2461 ctgcgtttag ccaatgtaaa tttccttttt ttctaactga taatccaaag cttgcgccat
     2521 aaagctctgc aagttgcgca cttaactttg tctgatccgg ataattcaaa ctatttgttt
     2581 ccaacatgct gctgagaagt gaacgtttcg tgatgacttc ttgttgcaga cgcgcagtaa
     2641 aacggataaa aatccggatc gttttgtatt gttttgttgg taagacatga agattcacgc
     2701 cttctgccaa ttgatacacc atatttatat gctccttttc acagttgatt gcccgcttac
     2761 tcttttaatg ggtcaacctg ccttttttac ttattcctct gcttgaaatt tataagttca
     2821 ggcaattcgc cgttttttct aaatgatgtt acactttata gtatagcata gatgtttttt
     2881 atcaaaagaa acagacggct aaaagcgtat attgtttttt tattttttat ttttcgatat
     2941 actaaagaca aataaaagaa gggagggtca ttcatgatca aagaattcaa agagttcatc
     3001 atgcgtggag atgtcttgga tcttgctgtc ggcgttgtta ttggtagtgc gtttacaagc
     3061 atcgtaaacc aagtggtgca aggcttgatc acaccactaa taggttggtt tgtcgcaatg
     3121 atcacaggta cttctgattt agaaggagca ctttcaattc ttgactggtc gccgacaaaa
     3181 ggcgtcacat ttgcgtttgg taacgttatt agtgctatca ttacatttct gatcactggt
     3241 tttgtcttat tcttagttgt caaagcggca aaccgtgcga agaatttacg ctccaaagaa
     3301 gagcaagtgg aagtggaaga aactcctact gcagaagatt atcttagtga catacgcgat
     3361 cttcttgcca aacaagtggc agaagacaat acacatccag tagagcaaac aaatctttcc
     3421 gatacttcta aataataaag aatctaaaaa aaagaaagcc gattttcagt gtcggctttc
     3481 tttttttaga ttcttaactt gctacttgtt cctttttcat gtcgatatgt ggaatatcat
     3541 cttccaaata cacatctgag attgccttga atccaaacga ctcataaaaa gcttgtaggt
     3601 attcttgtgc gccgatcacg attggttttt ctggaaaatt tttctggatc atgttaatcg
     3661 tttcttgaac aatctttctt cctaaaccat ctttacggtt gtcctgatga acaaggacac
     3721 gtccaaaatg gatactctcc gcttcttcat atactcgcgt gtaggcaagg atcttcccat
     3781 cagtatcttg aaaaaacgta tgccaagctt gtccatcaat gtcatcaact tcttgataag
     3841 gacactgttg ttccacaaca aaaactgcga ttcgttcttt gacgatcgcg taaaactctg
     3901 ctgttgacaa ttcttcaaat ttctttacct gatattccac taagcaaacg ccccctgctt
     3961 ttttattctt agcataccga ttcgcacaca aaaaagcaaa cgaaccttac gaatct
//
LOCUS       DANKHL010000223         4008 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000223 DANKHL010000000
VERSION     DANKHL010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.234"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(118..1623)
                     /locus_tag="PW101_002562"
     CDS             complement(118..1623)
                     /locus_tag="PW101_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2514933.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1620..2306)
                     /locus_tag="PW101_002563"
     CDS             complement(1620..2306)
                     /locus_tag="PW101_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2514934.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2499..3920)
                     /gene="gndA"
                     /locus_tag="PW101_002564"
     CDS             complement(2499..3920)
                     /gene="gndA"
                     /locus_tag="PW101_002564"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDL2514935.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1101 a          851 c          687 g         1369 t
ORIGIN      
        1 tattctattt aattgttcgc tagttttttt ataatgacat ccctgaattt ctcacttgct
       61 tgtattttgt accttattct ttgttggata tataaaaaaa caagtaaagc acatgcttta
      121 cttgtcttct gcctttgcga taggaataaa gattctaaaa attgtccctt gccctactac
      181 actttctgct aagatacgtc ctttatagtt ttcaaccagt tgtttagcga tagacaaccc
      241 cagaccattt ccacccttcg ttctagctct tgctttatcc actcgataaa aacgattgaa
      301 gatcttatta aggtcttctt tgggtattcc ttcgccaaag tcctgaatcg cgatctcaaa
      361 ttcattcaga gtggaagaaa tagagatatg gacttctttt cgtgtagttg aatactttac
      421 tgcgttatcc aatatgataa tgattaattg ttcaaagtga ttacgataaa tttgcagggt
      481 cacttctttt gtcaaatcat catctaaagt gatcacatag tctggatata atatcttgaa
      541 attattataa acttgatagg tgacttcttt tcctttagtt gtttcattcg cataatgaac
      601 atctacttgt tctgctcttg ataagtctag catctcttga actaagcttt tcatacggct
      661 gatttcttgc atgctggcgc ttaatgattc ttctaatatc tcaggatcat ctttccccca
      721 acgatttagc agactaagat gcccttcaat aattgcaaca ggtgtcctta actcatgaga
      781 tacgtcttcc acgaattgct cttgttgttc aatatacaaa cgcatacggt caagcatctc
      841 attgaatatt tcagctaaat ctgccaattc atcatttgtg tcgatttcag gcatgtggat
      901 atctgattgc ggatctttac gtatagtatc cattgtatct ctgagtacct tgagtggttt
      961 taagaaataa gaagataaaa agaaacctaa aatactactt aagatcaaag aaacaacttc
     1021 taaaacaata agagtcaata atagcttatt tcgaatatca tagaaagaag ataattcata
     1081 gaagctttgc gcatagccga tcttttctct tgtaccttta gaatatatag gctggataga
     1141 taaaaagcca gtttttcctt ctagtgtaac gatcgttgga actttacgtg tagtctgaat
     1201 caggtttcga ctgttttcat gggttttaaa aatcaagttc tcgtccaaat cataaacata
     1261 aagatccatt gatggctgcc ctagctcagc tataaaggaa tcgatcttca ttaagtttcc
     1321 ttcgaccagt gaagtatgat ctacgacctc atctccgtca tttgtcgctt tcgtcagatt
     1381 acgatacgta tttactaatg tcagttcttc atccgagtta gccaaacgag aagctacttc
     1441 tgaaatcgta cgctctacat tgtttctttc ttttgccaca atcagattga tagatgattt
     1501 ataggtaatg acagcgaaaa ttgtaaacac aacgaatatg aagaaagaac tcgcaaaggc
     1561 ccacttgatt gtcaaggagg gccctttcag ttctcttttt tccgattttt tcggctttat
     1621 cacgagcgca tcacataacc tgttccgcgt acagtttgga tatagctttc ctccccggga
     1681 acgtcaattt tgttgcgaag ataacgaata tatacgtcca ctacgtttgt ttcaacttct
     1741 gtttcgtatc cccacacttt atttagtaaa acgtcacgtg ctaataccac gttcacgttt
     1801 tccattaaag taagcagcaa ctcgtattct ctttttgtta gttcaattac ttcatttccg
     1861 cgacgtacga cacggttttc tttttcgata gtcaaatcgc gataagtaat agtagtttgt
     1921 ttagccacat ttttatcgcc ttcaatatcg attcgacgca acaatgcacg aagacgtgct
     1981 aataattctt cgatagcaaa tggtttgacg atgtagtcat ctgctccgtg gtcaagtcca
     2041 gatacacgat cgatcacaga atctcttgca gtcatcataa tgattggtgt atttttgact
     2101 tggcgtacac gtctgcagac ttctaaaccg ttcaattctg ggagcatcag atccaacaat
     2161 atggcatccc actcattatt taatgccgca tctaaaccag ttcggccgtt gtagtggact
     2221 tctgcgttat acccttcatg cttcaactct agctcgacaa atcgtgccaa gttcttttca
     2281 tcttcaatga ttagaatatt gctcatttat tattttatcc tttctattct actggctttt
     2341 taaaaactct atcattatac cgataatatg cttaaaaatc tatagtcatt ttatcatgga
     2401 tagcaatttt aaaaaagagc tgaagtctac tcataacaat cgacttcagc tctttcgtcc
     2461 tctttttatt ttctcatttc ctaacagccg aaagactatt attcttcatg gtaccatgag
     2521 taatggaaga ttccttcttt gtcgacacgc tcataggtat gtgcgccaaa gtaatcgcgt
     2581 tgggcttgga ttatatttgc aggcagacgt tctgctctgt atgaatcgaa gtaagcgatg
     2641 gctgatgaga aagtcggtac aggtacgcca gcttggacag ccaaggcaac gacgtctctg
     2701 actgattgct gatatttttt cgtgatttca acgaagtaat catctagcaa taaattttcg
     2761 atctcaggat ttttgtcata ggcatccgta attttttgca agaatcgagc tcggatgata
     2821 catccagcgc gccagatttt tgcgatttca ccaaatggca agtcccaatc aaattctttt
     2881 gaagcgacac gtaattgagc aaatccttgg gcataactca tgattttact gaaatacaac
     2941 gcttctcgga ttttttcgac caattctttt ttgtctcccg tgaactcaaa atcgttcgta
     3001 cgagacaaaa tcttgcttgc ttgtacgcgt tcttctttat aagcagaaat atagcgggca
     3061 aatacagatt cagtgatcaa tggaagtggc acacctaagt caagcgcgct ttggcttgtc
     3121 catttacctg ttcctttatt cccagctgca tcaaggatca catctactac tggtttacct
     3181 gttccttcat cgtctttacg tgtcagaatg tcagcagtga tttctatcaa atagctgtct
     3241 aattcgcctt gattccattc tttaaagata tcagccattt cttctactga aaggtttaaa
     3301 atattcttca ttaagtcata agattcagca atcagttgca tatctccata ctcaataccg
     3361 ttgtgtacca ttttcacata atgtccggct ccatttgggc caatatatgt gacacatggt
     3421 gctccgtctt ctgcttttgc agaaatttgc tctaagattg gtgcaactag cgcataggct
     3481 tctttttgtc caccaggcat gattgaaggt ccttttaaag ctccttcttc accaccggaa
     3541 acgcctgtac cgataaagtt gatgccagag ttggctaact cttcattacg acgcatcgta
     3601 tctttaaaga atgtatttcc tccgtcaatc aaaatatcgc ctttatcaag gtgaggaagt
     3661 aactcttgga ttgttgcatc tgttgccggt cctgctttga ccattagcaa gatccgacgt
     3721 ggtttctcga tagattctac aaattcttcg attgtatatg ttgctttgaa atttttatct
     3781 gggtgttctt ctactacagc ggttgtttta gcccctgtac gattgaataa agccactgaa
     3841 tagccgcggc tttctatgtt caaggctaga tttttaccca taacggccat accgacaacg
     3901 ccgatttgtt gttttgacat atgaactcct cctacatacg ggatttctcc ctaatctctt
     3961 acattatagc ttaacacggc tacattgcaa actaaagaac aacatatt
//
LOCUS       DANKHL010000224         3970 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000224 DANKHL010000000
VERSION     DANKHL010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.235"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            719..1759
                     /locus_tag="PW101_002565"
     CDS             719..1759
                     /locus_tag="PW101_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL2514936.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            2583..2915
                     /locus_tag="PW101_002566"
     CDS             2583..2915
                     /locus_tag="PW101_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDL2514937.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3534..3875
                     /locus_tag="PW101_002567"
     CDS             3534..3875
                     /locus_tag="PW101_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HDL2514938.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1453 a          507 c          717 g         1293 t
ORIGIN      
        1 tctcttatta agaactacct aatttgggta gttcttattt tttacatttt aagtagtatt
       61 taattgcaat ttggtagtat ttttttatta tcttattctt agaacggtag tacttaatat
      121 aaatataggt agtatctaat ttaagtatta atggtagtat ctaaattaac aggtagtatg
      181 taactacttt aaagtaattt ttttcagatt aacacagtag tatcttatta aataagttaa
      241 ttttaatggt agtacataat tattatatat atctgacatt ggtagtactt attgtatttt
      301 ttagtagtat ataatctatt gttttctagc ctaaagcctt gtcttataag aataatataa
      361 tatatactac tgaaataagg tagtatatat tactagtaat ggtagtatgt tttatatgca
      421 taaggtagtt cttaatttat tttttgtttt gacactggta gtacttaatt atctttaatt
      481 ttaaaactaa attttattca tacgtttttc tggtagtatt taattataaa taatatttat
      541 ttaaagtagt tcttaatgac tttaaaggta gtacataatt cattttattt ctgattttag
      601 gtagtattta attaaaaaac agttttaatg cgtttgcatt ttttatttat ctttgctatt
      661 ttcgaaaaga gtaggtaatt ttacataaat aaaacaacgt gaaagtagag gatctacaat
      721 gactgatttt aaatttttta aggcggatag agtttacaac gaattatttt atcaatttcc
      781 aaaagtcttt attgtttctg acgaatacaa aaaaatgaaa gattcaacta agattgccta
      841 tatgctttta aaagcaagat tagagatcgc aatcagcaaa cggcaaatcg atgaagaagg
      901 taatgtttat tttacttata cgacaaatga actatgtaga gtattaaact gccaaaaaca
      961 aaaagcgata gcaatcaaaa aagagttgga atcctttggt ttattattac aaaagcagat
     1021 gggatttaac aaacagttag ggaaaaataa tcctaataga ctatatctag cagaattaaa
     1081 agtctcagaa aatgatatct acttactcga aaaatttgat agagagaata gggaaaacgt
     1141 tgataaatca gagggtatga aaatcatacc caccctcgac gaaaaatcag acgctgaatc
     1201 ccttggggct caagagggta tgaaaatcat accgtgccaa aacgttgata aatcagaggg
     1261 tatgaaaatc ataccagaac ttaataataa tatattagac actaatagac acaatataga
     1321 cactgaaaaa gaccgcctac aagatcaatt gttgttagac aattttgaga caattatgac
     1381 aaacgacagc attgctacgt ttgtccctga acgagtatta aatttgataa aaacattttc
     1441 ttcaagttac agtgaagctc aaaaaaccgt ccagactatt cataatgcaa agaaaaaagc
     1501 tgaaatagaa agtgatattt cgatagtttt tgaagaactc gatagttatt atgtcaatgc
     1561 agaacaagaa ttatacacga cactgttaaa agcctatcaa aaattaaaaa ccgaaaaagt
     1621 cgaaaatatc cagaacctga tttttgtcta tgtaaaaaat tggtttatcg aaaaaccaat
     1681 agctgctaaa gtatcaagtg aaaaacgttt gaattatgca agctccccaa gcactattac
     1741 gaaagactgg ttagagtgaa actttactga caaagtgatt gttgcttcat ggagtaacat
     1801 taaaattaaa aattttttat tattctaatt atattacgaa tattacaaaa taatagctat
     1861 tttaatagaa tttataagtt aaaatattat gataaaaatc acaaacgtag ccttttacgt
     1921 cataagtgtt aaaagaaata attaatttca aatatagaaa agggaacgta tgtttgcata
     1981 ctcctttttt ttgagttata ataatacgcg aagagagata atttcattat ttaaacaagc
     2041 taaaaatctt gatattactt cttttcaggc aatacgaata ttattcgctt acattttttg
     2101 tataaattta attgtctgct atgcttgtga tgaattgatt ttcgttttgc acttttggat
     2161 gataaatttg gacaattgaa aatacaaaaa aaagaaaggc ggtatgaact cggaaggctg
     2221 gtaaccttcg tcagcgttca tacacggcat taagatttgg cccttaatgc gttaaccttt
     2281 cagtgttacg gtattacccg caatggattt agtataacag atattcaaaa gtgataccac
     2341 tttttttggc tgtgttaagg ttaaaaatac gtgatattac gatgaatcta tgcctaagca
     2401 taatgttata aaaatgtccg tgtggctctt tgccgtatga cgaacgctga aattagaggg
     2461 aacgcatgtt tggatgcgtt ccctcttttt tgtttgtcat gaacgaaaat caattctcta
     2521 tttttgaaaa ataacaagaa aaacttgtta aaaatattta aagaaattgg gtggtttatt
     2581 ttatgtcata tacaggaatt ttgagtctca aagatatttg tcactacggt aaacggtgta
     2641 ccgctacaga gaaaatcacg aagaaactat caactggcca aagtaaaacc gttgtgcagt
     2701 gtaagaaata tataatccag aaagacaaag tctcagagga aatgatttat tacattggga
     2761 aacaaaagca aataatttta aaagatccta tccccttaaa agagctatat ccaacaatca
     2821 aacatgttta tgaccaaaac ggtgtactga ttggtcgaag aaaaaatgga gtcttgcgtt
     2881 gtaccgcaaa agggatgggg cgtttgatcg gctgaattga tcattctcat aacttgtatt
     2941 ttatcgtttt tcgcttgctt gaaaatattg tcgagacttc taaaggaact tagagtcact
     3001 agaaaaataa ggagtagact tgatctatca acaattccct tcggggagtg acggggtagg
     3061 ttttaacgat tggtaacgta aaaaattggt caatcgagta aatgcgcttg tttggatagt
     3121 ggtgaaatga atttgtgtaa ataataagcg cagagtaaac aagagggaat ttagaagtta
     3181 gacaagttag aatagcgtct agattaatca tgagcagcta ctaagggatc tagactttga
     3241 cgcaaactca tggaataagg agtaggtctt ctcgtaatgg ggaggacgca caagaaattg
     3301 tgtcgtgtgc tgcagtacat gtagatactt gcgaggacgt tgattattgg atcaatcgag
     3361 catttaccgc tgtctactag ggtttctcgt ttcggacgcc ataaacgagt cgaaattttt
     3421 tatgtatttt taagtaaacg aaaaacgata aaatatgaac aagtcctgaa taaagtgaaa
     3481 aaattgatat aaaacataga gaaaatgaaa tttacgtaat ggaggaatga caaatgaaaa
     3541 attatgatcc gaatatacga ttgggcacac atactattaa ggtttctttc caacgttggg
     3601 attacaaagg atttgtgacg tttagaaggg gcggaaattg taaaggatta gatgttttag
     3661 cattagacga ggatgattta tacgatcaaa cattgactga taatccaatt ggattcggct
     3721 tgctacctga agatgatgaa ggtaatgaat ggtttaaaat gacgcttatg aatgataatg
     3781 gagacgaatt atctgttgaa gatacttggt catatttgag tgattatatt gtcagctttg
     3841 aaatcattga atttgtagcg gataaagaag aataaaaact ggaggaataa attttacaga
     3901 taaaggctta gaaggagtca cgacgattaa taaaaaggta gtattacctt aggcattgag
     3961 taaggaattt
//
LOCUS       DANKHL010000225         3931 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000225 DANKHL010000000
VERSION     DANKHL010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.236"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..156
                     /locus_tag="PW101_002568"
     CDS             <1..156
                     /locus_tag="PW101_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDL2514939.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="PW101_002569"
     CDS             175..1452
                     /locus_tag="PW101_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2514940.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="PW101_002570"
     CDS             1462..2118
                     /locus_tag="PW101_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2514941.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="PW101_002571"
     CDS             2118..3494
                     /locus_tag="PW101_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2514942.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3624..>3931
                     /locus_tag="PW101_002572"
     CDS             3624..>3931
                     /locus_tag="PW101_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2514943.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     "
BASE COUNT         1585 a          418 c          688 g         1240 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 tagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta a
//
LOCUS       DANKHL010000226         3851 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000226 DANKHL010000000
VERSION     DANKHL010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.237"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            29..685
                     /locus_tag="PW101_002573"
     CDS             29..685
                     /locus_tag="PW101_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDL2514944.1"
                     /translation="MIVILGFAFKLDSILIVMVALVVTALTGGLGIQGMLELLGTSFV
                     NNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGIFRMIFAAFNV
                     SFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCWFFGQVLFIGG
                     AGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKLAKKYYPGKGQ
                     "
     gene            687..1631
                     /locus_tag="PW101_002574"
     CDS             687..1631
                     /locus_tag="PW101_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDL2514945.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1830..2474
                     /gene="pcp"
                     /locus_tag="PW101_002575"
     CDS             1830..2474
                     /gene="pcp"
                     /locus_tag="PW101_002575"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDL2514946.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2553..3830
                     /locus_tag="PW101_002576"
     CDS             2553..3830
                     /locus_tag="PW101_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514947.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         1228 a          675 c          857 g         1091 t
ORIGIN      
        1 ggaaaattat cttagccttt taggcgtctt gattgttatt ctcgggtttg catttaaatt
       61 agactcgata ttgatcgtaa tggttgcttt agttgttaca gctttgactg gcggtcttgg
      121 cattcaagga atgttagaac ttttaggtac aagttttgta aacaatcgcg ggatggcgat
      181 ttttatcatc attatgttgg caacaggaac gcttgaaaga aatggtctga aagaatcagc
      241 ggcttccttg atcaagcgat tcaagaaagt atctgctggt atgattgtag atatttatgg
      301 gatctttcga atgatctttg cggcttttaa cgtgagtttt ggtggagttg ctggatttgt
      361 tcgtccaatc attttaccga tggcattggg gaccatcgaa tcaaaagggt tagaagtcca
      421 tcctaagcat gaagaagaac taaaaggtat ggcttcagct atggaaaatg tctgctggtt
      481 ctttggccaa gtactattca tcggaggagc aggggcgttg ctagtccaat caacactgaa
      541 agatctagga tatgacgtaa cattgggaca gcttgcttta gtggaagttc cagtggctat
      601 cacggcttta gttgtggcaa gtgtctactt ctatcttaaa gaaaagaaat tagcgaagaa
      661 atattatcca ggaaaggggc aataagatgg acttttttac aaatcctgat attttattag
      721 gcgataaatt actggagatc ctttatatcg tgatgggact cgtattgatt tacactggag
      781 taagaaattt aacagataaa accaatcctc atcgttatgg gtcagccttt ttctggacgg
      841 tcttaggggt agttattgca ggaggaagat ggcttccggc aatcgtgaac ggtggcttga
      901 tctttgcgat gaccatacca gcaattgcta agaaagtcag caaaggagaa agccggcttc
      961 catcaaaaga atatatggaa aaaatgtcag acaaactagg aatgaaaatt tttattccag
     1021 ctttgagtat cggtgtcttt gcgatcttgt ttgctctatt taccaattta ggtgctttag
     1081 tcggcgtggg cgtcggtgtt tttgcagcca tgctaatctt gatgttcttt tctcgagata
     1141 atcgaccaag tacattttta gatgatgcag cagatatgct gggcacagta gggccgttaa
     1201 gcatgttgcc aatgctttta gcttctctag gtgcggtatt tacaagcgct ggagttggga
     1261 cagtgatttc caatgcagta ggtacgatcg ttccagaagg aaatgtgaac atcggcatca
     1321 tcatttacgc tcttggcatg gtgatattta cggtaatcat ggggaatgca tttgcggcca
     1381 tcaccgtgat gacggttggg atcggtgcac cattcgtatt tagtcatgga gcgaatccag
     1441 cgttaatagg aatggttgca ttgacttgcg gtttttgtgg gaccctgctg acaccgatgg
     1501 ctgctaactt caatatcgtg cctgtagcga tgctggagat gaaagacaaa tacggagtga
     1561 tcaaaaacca gttgtttatt gcgttattta tgctagtttt ccaaattgct tatatgattc
     1621 tatttaaata atagaagaga ggggttgtga cagaataatt gtcgcaacct cttctaacat
     1681 tgtagtggaa cactattttt ttataagaat atattaagtt tgatctgtta tgttatttaa
     1741 actattgttt cctttattta cgctgttcaa ggaaaatggt atgataaaag ataaccatat
     1801 gttaaatcaa tcggacggag gaacaacaga tgaaagtact agtaacaggt tttgatccat
     1861 ttggtgggga caaagtgaat ccagcctatg aagcagtaaa aaaaatgcca gatgaaatca
     1921 gcggtgctga aatcatcaaa gtggaagtac cgaccgtatt cgaaaaaagc agtcaagtag
     1981 tcaaagaagc catccaacag caccaaccag atgtcgtgat ctgtgtcgga caagcaggtg
     2041 ggcggtcagc agtctcgttc gaacgtgtcg caatcaatct agcagaagca agaatccctg
     2101 ataatgaagg aaatcagcca ttcgatacgg ctttagaaga aaacggtcca gccgcttact
     2161 ttacctcatt gccaatcaaa gcaatgacaa aaaatgtaca agatcatggt ctaccagcgt
     2221 acatctctta tacagcagga acatttgtct gcaatgatat catgtatcgt ttgcttcatt
     2281 tgatcgaaac acaattcccg gcaattcgag gtgggttcat ccacgtgcca ttctcacccg
     2341 atcaagtgat cgatcgtcca gtgggtacgc cttctatgtc tttagaagac attgcttctt
     2401 ctttgactta tgcagttgaa gcagcagtag aaaataaaga agatatttcc ggaaatgccg
     2461 gaacaactca ctaatttagc aagaaacact ggctggttgc taacgttatg ttagtagcca
     2521 gctttttgta attagaaagg aaagaaacag ccgtggaaga atggcaatcc gtctttgaag
     2581 aatggtttcc aaaagaaatc agcaaaagct atcccatcaa aatttctaaa caatacacaa
     2641 gcagtcaacg ttgggagatc tatgcaaaat taacgaaaaa acagcgggaa ctagtcgata
     2701 agcaccgccg atatctaatc agctctcgtt ttatggaaga acattattta gctgcaacag
     2761 attgggtgtt ttctgacttc aagatcaatc cgttttttcg aacaaaacga agccagcaaa
     2821 aattgtattg tgagtgtggt agagaattaa aagtgcaata catcgttaaa tcgccaaaaa
     2881 ccggaaagat cctaaaacta ggcatcaacc actttgccga tcacctgcat gtctctccga
     2941 cagtggcagc ttcgatccat cagggaatga caaaagttga tctggcctta gacgaattat
     3001 tatggcttaa gcagaagaat atcgattttc ctgaaggact atggcaaaaa tactgttttg
     3061 tcttgtacca aaatcgtcgg atgaaacagc catatttacc ggatataaaa ttagctcagc
     3121 ggctcgcaga atttcgccaa gtagaaatgc cgatttacat agcggattat caagcattgg
     3181 aaaatgaaat caagaaaata agtgaacaca tcaatgggca gtcaaaaaag cgacagataa
     3241 aaaaagaact atttgacgac tttgcagaag aattagtaaa ggatgtagaa gaattcttga
     3301 tcaactatcg cgcattttta cgtaaagatt ggcaatccat cgtatatgaa gaagtgccag
     3361 tacatcctaa tgcttatttt gaaacattta tttccgtgtt gagaaagaca aaacgtcaac
     3421 gaacaccaga agtcactgct cagatggaat attttgcgaa aaaccaacga ttcatccaac
     3481 caaaaatcta tctatttatt tggaagcaat attgtcgcta tggttttaca gaagggttct
     3541 tcgacagcat tcctcggata gtcagaaatg gttttttaaa agtattgcgt aaagaacgag
     3601 aagctataca atctgctgat aagaaagacc gtacagtctc aaaagaaaaa tggcaactag
     3661 tagtgaaaga tatccaatct ggaaatgtgc aagagacaat cgacaaatgg aaaggaaaac
     3721 attatcgttt tactgaagct cagaaacaag cgttggaata ttatcagaag ttagaagaat
     3781 cactgcgttt caatgatgag gcaagaaagt atctgaagga attactataa aaaacatact
     3841 aagattagag g
//
LOCUS       DANKHL010000227         3843 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000227 DANKHL010000000
VERSION     DANKHL010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.238"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            25..873
                     /locus_tag="PW101_002577"
     CDS             25..873
                     /locus_tag="PW101_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514948.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(1044..1385)
                     /locus_tag="PW101_002578"
     CDS             complement(1044..1385)
                     /locus_tag="PW101_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HDL2514949.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1471..2178
                     /locus_tag="PW101_002579"
     CDS             1471..2178
                     /locus_tag="PW101_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL2514950.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2165..2488
                     /locus_tag="PW101_002580"
     CDS             2165..2488
                     /locus_tag="PW101_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL2514951.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2577..3614)
                     /locus_tag="PW101_002581"
     CDS             complement(2577..3614)
                     /locus_tag="PW101_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2514952.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
BASE COUNT         1114 a          800 c          691 g         1238 t
ORIGIN      
        1 agtgtttcat gaaaggagaa ctagatgaat aattggcttg agtattttcc tgaaaacgtt
       61 ttggaacgcg gttacagcta tcatctgcac ggttttgtcc gtcatttgaa ctatacaagc
      121 aagtacttgt ccgcaacagt ttctggcacg gaagattata aggtagtgat cacttgggat
      181 gaaaagacca acatgacttg tgattgtcta tatgccatag aaggaaaaaa atgcaaacac
      241 atggctgctg tcttattcgc ttacgaagaa cgtccaataa aaaaatccaa ctactcactt
      301 tccgaactct cgtcccttgt ttcctccgct agttcctctc ttgtacggga gcttttaaca
      361 gagattctta tagaacatcc gcactttatc gaacgcttca aagttaagat gccatttcat
      421 gcaataaatt attcagataa attaacaact atcatccata aatacgatca tattatcaag
      481 aaaaacaaaa acaggaaaac agcgaagttc atcatggaga tgcgaaagtt catccaagaa
      541 gcagttgaat cgcttattca gcaaaatgcc tatcttcccg cattcgaatt gatcaatgaa
      601 gtgattgcta ctctcgaaac attctattgg gagcctgagg atgaacgaac ccttcttctg
      661 atagaggact gttattattt atggaaagaa ttattagcag aagcacctca tgcagaaaaa
      721 aggcaaatgt tctcttggtt cgtctgccaa gtcgatcata ctgacgcctc ttattcaaaa
      781 cgctactcta taaaaatcct gaaagaagac tttcgggaaa aagaattttc taaccaaaag
      841 aaaaaaatag ataaaaaact aaagaaacgg taaagaaaga cgatttcaat gaaaaatagc
      901 aagtaaacga taacaaccat cctaaatcca caagaaatac agcagacctt gctttaaaaa
      961 cacgaatcat gcaaaggatg aagtaaagaa gacttaacgc gtacccaata ttagtcattt
     1021 gactattagg tacgcgtttt gttttataag tcatgacaaa tttccttcgt tgctggatag
     1081 attttccggc attgttgata gattttatga tacttttcta cattttgtaa gagaggttcc
     1141 actgcatctg tatattggat aaaattctga caacaacttt caggtgtgtc gaaccaccct
     1201 aaccctagtg cagcaatcat tgttgcgcct aatgcaggtc cttcctccac acttaaacaa
     1261 tagaccgttg catcgaaaat atcagcttgg atttgtagcc atgcaagatt tttcgcgcct
     1321 cctcccaccg aaacaatacg tgtaaatctg cgacccgcta gttctttacg tttcaatccc
     1381 accattgaca aatgctcatt aaaaatttag aatattcaca agtttaacga caattatgaa
     1441 aatttatgtc gtgctaaagg agttttgtta ttggatgaaa agttagatat aaaaactgcg
     1501 atcaaagaca cgttacctac cgttttcggt tatatcggta ttggacttgc atttggtatc
     1561 gttgggaaag ctgccggatt tcatccacta gtcgtcacgt tgatgtccct gctggtctat
     1621 gctggttctg cccaatttat cacagtcagc atgcttgcta gtcacagccc attgctttcc
     1681 atcgttttct cgacctttct agtcaattcc cgaatgattc tgatgagtat gacgattgct
     1741 ccttatttca agaaaaatcg gctgcttcaa aatctgctga ttggcacatt gctgacagat
     1801 gaaagtttcg cactgggaat gaataaattg aactatactg gacaaaagtt aaactttcgt
     1861 tggatgaata cagctaattg gatttcttat ttgacgtggg tgggttcttc tcttgttggc
     1921 gcacttttag ggaattttat tactgatccg aaaaagtttg gtttggattt cgccatcgtc
     1981 gctatgttta tcgggttact ttatcttcaa gtcatttctg atagaaacac ctctaaacga
     2041 ttacagttga tcctgattgg tctcactttg attctggtat atgtcggact gatttttatc
     2101 ccaagcaact tagtgattgt agttgtaaca ctgattgggt gcggattggg ggtgtggatc
     2161 aaacatgcct tcttttgagt ttacattgct gactatttta ggttgtactt tagcaacttg
     2221 gctttctagg gtcctaccat ttgtcttact gaaaaaattc gatttaccgc aaccgttgct
     2281 tgagtattta agttttgtcc cgatcgtcat tatgtctgct ttatggttca gcagtctatt
     2341 tacccaaaat atcggacatc tgccgcagat caatctcgaa aatgctttgg cttcagttcc
     2401 tacactttta gctgctattc tttcaaaaag cttattggtg atcgtcttgg caggaattct
     2461 ttccctttcg ttgattcgct tattataaat aaaaaaacaa gagcttatgg caaattactg
     2521 tcatgcgctc ttgtttttat caaagtcaaa taagaaaaaa atcggcgtat tcttgcttat
     2581 ccgttgaacg gaccactaac aacgactttt cctaacatat gtcctgtttc tacgtctttg
     2641 gttgcttgtt tcaagttctt cgcattcatc ccatctgaat agaccttgct caatgtactg
     2701 cggataatcc cttcatttgc taaagcagca atgtgcgcta aagcttctcc ttgcgtttcg
     2761 atttcatagt tatagtctgt tttagcgaac atatactccc agtcgaagct gatagattgg
     2821 tttttccaaa cacctatatc taacggctct ttgatcccta cgatcgttcc cacatgtcca
     2881 aatggtcgga caaggtgctt gatttgatca aagtagcggg taatatcgaa taatacagcg
     2941 acatactcta cttgatcgat tcctaattca cgaaaacttt tacttaagtc gtgatgataa
     3001 tcgattggat agtctacacc tgtttttttc agccattcaa aattcttcgg acttgcagtc
     3061 gcgtagactt ctaggccagc ccaatgcgct agttgattca aaatcgaacc gacgcccccg
     3121 gatccgttga tcactaagat ttttttcccg cgattggcat tttcttctgg agttagacca
     3181 aatttttcaa ataacaattc ataagcggtc aatgaggtca acggcaaggc tgcagcttct
     3241 tcatctgaaa gattttttgg tgcaagagcg acaatacgtt cgtcaactag ttggtattct
     3301 tgattgcttc ctgctcttgt tgttgtacct gcgtaaaaaa cgcgatcgcc gacagaaaat
     3361 ttttgcactt cgtcacctac agcaacaact ttcccgactc catcaaaacc taagatacgt
     3421 aaagcatttc ttatcccgtt atcttggcga agtttcgtat cgacaggatt aacagatact
     3481 gcgttgactt ttaccagaag atcccgtgaa ctgggtacag gtatctgttc tttttcatca
     3541 ataaagcttt ttggatcatc gatcggcaac ccttcataaa aaccgactac cttcattaat
     3601 tccttttctg acatacgaat tcctccttag cttcatcaag ttggagcatc catacagtgt
     3661 tcatttcaat atatatagta tgctatttgt tccacctttt ttattcatca taattattct
     3721 cttacttaaa gtaaactatt tgcacttttt tggttaaaaa agaggttgcg acaagacttt
     3781 tgtcacaacc tcttataaac ggtgttctcc taccaactct tcggtcacaa gtcaaaatat
     3841 tca
//
LOCUS       DANKHL010000228         3834 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000228 DANKHL010000000
VERSION     DANKHL010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.239"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            352..1041
                     /locus_tag="PW101_002582"
     CDS             352..1041
                     /locus_tag="PW101_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2514953.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="PW101_002583"
     CDS             1028..2236
                     /locus_tag="PW101_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2514954.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="PW101_002584"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="PW101_002584"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDL2514955.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1261 a          662 c          775 g         1136 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agta
//
LOCUS       DANKHL010000229         3755 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000229 DANKHL010000000
VERSION     DANKHL010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.240"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..235)
                     /locus_tag="PW101_002585"
     CDS             complement(<1..235)
                     /locus_tag="PW101_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514956.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSS"
     gene            complement(533..1507)
                     /gene="sgrA"
                     /locus_tag="PW101_002586"
     CDS             complement(533..1507)
                     /gene="sgrA"
                     /locus_tag="PW101_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HDL2514957.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1703..2542)
                     /locus_tag="PW101_002587"
     CDS             complement(1703..2542)
                     /locus_tag="PW101_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2514958.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2727..3614
                     /locus_tag="PW101_002588"
     CDS             2727..3614
                     /locus_tag="PW101_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL2514959.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLIDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
BASE COUNT         1251 a          763 c          558 g         1183 t
ORIGIN      
        1 gagaagaaaa ctcgactttt atcatcttgt tttctgaaag aaaagataat ttatattctg
       61 gatacctttc gcaaaacgat tctgcttgtt gaatcaccga attcatctta gccagggtgt
      121 agtttagatc taaagcgata tccttcacca catataattt ctcgtctctt tcataacaat
      181 agttcaaaaa ttgatatact tgcctttcat tgatatcaat aaattcttca aacaaagtac
      241 atactccttt ttatctatta tttagactta ttccgacagc tacatgaata acgtacaaat
      301 agattcgtta ctgatttttc agagatacta ccatgcgcag tgtgcattgt caattattct
      361 tgctcttatt ttccatttgt gttcctgtta tttcctgttt ctcaaatatg ttagtaaaca
      421 aaaatagatc tggcaaagct acaacttcgc caggcaatac agcactctcg aatgaaatat
      481 tcactcaaaa atctatcgtt ttattcaatt ttagatctgt tgcttttatt ctttatttca
      541 ctcgatggaa gagagaacga aaatagcttg ctcctacaat caaaatgagt aacacaccca
      601 tccatgtaat tagcttgttc aaggttctac taccagtttc tggtaaatcg ctttttctat
      661 tatctgtttc tttcgctttt tcttgatgat caattccacg agaaacaaaa tcaggctgtt
      721 ttagcacact ttttaggttg ctccctgcat ttctcttttg ttccttagtt ggtatgatag
      781 gtaccaaatc aagttcatta tttacttttc ctgtgctgct ttcgctgctc gtgcttcctg
      841 tgctactctc actactcatg cttcctgtgc tactttcact actcgtactc tccgtactgc
      901 tttcgctgct cgtgcttcct gtgctacttt cactactcgt actctccgta ctgctttcgc
      961 tgctcgtgtt ttctgtgcta ctttcactgc tcgtactctc tgtactgctt tcaccgctcg
     1021 tgttttctgt actgctttca ctacttgtgc tatccgtact gcttggcggt ccaacaactg
     1081 tccaactgct tggtttcaaa ttctccgtga tcgtcatctg tccatcataa aaaacaatca
     1141 aatcaacttt atctttactc ttgatctcat caatcgatag ttctgcagaa atcattattg
     1201 tatactcaat accagcggtc cattgtacgt cttttaaata aacttcccca ataacttttt
     1261 ctgaacttcc attaccatcc caaccatatg ttagaacagc atgtacatca ctagcattca
     1321 cgcttttgtt tggtttgact ggaatagtaa tgacttgttg tgtcatgtca taaacaatcc
     1381 gatcagtttt cacagtaaat gaaagattct catttgcccg ttcatttgca gaagtactga
     1441 ttccaatcat tgctgcaatt atactcagcc caagaataac aaataatacg gtcgctgttt
     1501 tcttcatttc tatcactctc catataatta ccttctattt gataaaggaa ttatataaaa
     1561 tgaaaatgat gagaacacga cagtcagttg tgaaaatcta acaaaagatg tcgtgtattc
     1621 gcttgaattt gtttactttt tcgtttaatc aatgacagac ttaaaaaagc aagaccgata
     1681 tcagccttgc tttcttcatc ttttattgat aaacagcagt gtatttcttc atccgctttg
     1741 ttttatatgc atagagtaac aaaccaacaa tcaagactaa caagaataat gtagttgata
     1801 tagatgcttc agccccaaag tttcccccag taattattgt attttgcgat tggatcagag
     1861 tggagaaaaa accttgttct tgcgttccgc tgactggtat acccaaaata gctccttgtg
     1921 tccaattcca agtaatatga gcaccaatag ccgtaaataa acttcctgta ctgtagataa
     1981 gtgcgctgaa aaagcaacct gctaaaaaga tattgaaaac tgccagaaat gtcatgttag
     2041 gattcaatcc gtgcaaaaga gcaaataatg cagcagatcc aatcattgct attaacggac
     2101 caaatctaag catctgatgc tgaaaaaagc cacggaataa aagctcttct gatgccccca
     2161 ctgcaataaa taaaataaaa ctcgttatca gcggcacaat gtttgcgttt cctgaatcta
     2221 accgatacgc ccccgtcaaa tataatggac tagtaataag cagaaacact ccaattccaa
     2281 caacgagacc tcccgctaaa tacccccagt cttttcctcg aacagaaaca ccataatctt
     2341 ctattctttt tccttcgacc ttactagcaa gaaataaagc tagtaaaaaa acagctaaat
     2401 gtccgaccgt accggcaatt gctccaatcc attcactatt tccaataaac atatccgcaa
     2461 gtgcattatt gacaagaatt cctaaaacaa aaccaactaa gactattaaa acagatccaa
     2521 tcaaccgaat aatcctgatc atctttcatc cctcctttat attaagttta acaacccttt
     2581 tctttcttgt ctcgtgaaaa ctatgtaaat gttctccatc atagagagaa tagattttta
     2641 cgaactaaga atatctacag caaccttacg aaaatgaaag actgtctccc taattcttct
     2701 gttagactgt ttttaataag gaaaggatgg gaaatttttt gaaaaaacaa ctactaatta
     2761 tgtgcctttc agcatgtgca attttactat tctcaggttg tagcaagtcc aatgaaacga
     2821 ttgcaacgtt taaaggtgga acacttacag ttcaggattt ttatgataac ccaaaagtta
     2881 aatcaaataa tcaaacacta ttaaaaaaaa tgattgtctt caaagcattt gatgatgtat
     2941 acggaaaaga gatttcttca aaggaagtaa caaaagaata taatgaacaa atcaaaaaac
     3001 tcggtccaaa ctacaaggaa caattaaaag cagttggcca aacagaagag acatataaga
     3061 aactctttaa acagatgctg gctttccaat acggtttgaa agccaatgta aaattaatag
     3121 ataaagatct agacactgct tggaaagaat tctatccaga agttagtaca cagatcattc
     3181 tattttcgac taaagaagaa gcagataaag caaaaaaaga agcaaatgaa ggagaaaatt
     3241 tctccaagct tgttcaagct tatggaaaaa ataaaacatt aaaggaaact gatggaaaaa
     3301 tgaattttga ttcaaccaat ccagagattc ctactgaagt aaaaaaagca gcatttaaac
     3361 taaaaaacgg ggaagtttct gatatcattc ctgtaacaga tccaacgact tatcagcaaa
     3421 gctattatct agtaaaaatg gtaaaaaaac aggataaagg ttctaataaa gacaaataca
     3481 aatctgagct agaaaaaatt gctaccgaag caaaaactct tgatacggaa ttcatggata
     3541 aaacaatccg taaagtgatg aaaaaagaca atgtaactat taaagatcct tatgtaaaaa
     3601 atatattcaa ataaacaaat ttgacccaat tggggatgtg gcaaaatttg agaagtgatt
     3661 tttggaaatt ccttcttatt tttgaggcaa accacttttg ccacaacttc atcggggcct
     3721 ttttttattt gtaccaaaac aaatcactgg atttg
//
LOCUS       DANKHL010000230         3720 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000230 DANKHL010000000
VERSION     DANKHL010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.241"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..355)
                     /locus_tag="PW101_002589"
     CDS             complement(<1..355)
                     /locus_tag="PW101_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514960.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRTF"
     gene            complement(615..1163)
                     /locus_tag="PW101_002590"
     CDS             complement(615..1163)
                     /locus_tag="PW101_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514961.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1164..2021)
                     /locus_tag="PW101_002591"
     CDS             complement(1164..2021)
                     /locus_tag="PW101_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2514962.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2307..2593)
                     /locus_tag="PW101_002592"
                     /pseudo
     CDS             complement(2307..2593)
                     /locus_tag="PW101_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2583..2912)
                     /locus_tag="PW101_002593"
     CDS             complement(2583..2912)
                     /locus_tag="PW101_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2514963.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2989..3261)
                     /locus_tag="PW101_002594"
     CDS             complement(2989..3261)
                     /locus_tag="PW101_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL2514964.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3261..3524)
                     /locus_tag="PW101_002595"
     CDS             complement(3261..3524)
                     /locus_tag="PW101_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2514965.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1092 a          754 c          473 g         1401 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat actctatttg agagtcatct aattggctca
      241 taatcctttc tttttggtta gtatctgcag ttacttgttt agtatgagcc atgaacccaa
      301 acatactcac cccaacaacc aatcctaaat atattttctt tttaaacttt ttcatctaca
      361 tctcctccta atagttttat tttagagtta gctctattac ttatttacta actcaacaac
      421 agattatcat acacttgtta atgaaatcac aatatctcat taaaacacac cctaaaatca
      481 taaccgttcc tatgctcatt ttttggattt tttaatcacg taaccagaaa aataaatccc
      541 ctctatttca cgcaaatcaa agaagaacta agtaactacc gtttaaaggt agttacttag
      601 ttcttctttg attattatct aactttatta ttcattcccc accctttgcg cagtgagtct
      661 tcggtatctt gttttatctg actggtagtt cttattattt ctccacgatc atctaattct
      721 ggaacaattg gatatttttc tctgaacaag tctagttctt tcaccacgtc attaaacaaa
      781 tcttgcttat cttcttgcgt aagattctgt ttaatatact catctaaagc ttttctaagc
      841 aattcatctc ttgtcatatt agtctcatac ttttctacta aaactctgga agtcgcattt
      901 aacaaagtcg acagttttaa aggatgcttg gtggaataaa ccttatcaaa ctttctgatt
      961 ggtgcgccaa ctttttttgt tcttcctcta ctctgtcttt caacattgga ggaagttttt
     1021 atttcttgaa cttggtgcac ttctaattgc ttattttcag atggtgtaat tgcttgctgc
     1081 tttttctttg cttctgcttc tttttttatt ttggcgagtc ctttgttgtt cccaaaaata
     1141 tcaagtcctt tgatgtcagc caattactcc acctccgcat accaattctc gtgttcttcc
     1201 aattctttca aaatactgat aaataattcg tggcttttgc gatcccattg ttttaagaga
     1261 cctttctttg tcttctccat cgtaatacct gatccgtcgt atcgttttaa gcgcgcattt
     1321 ttgagtacaa catttttaat caaatgttcc ccataaatct cttgggcttg tttatacatt
     1381 tcctgatcta ctgcgtcacc gctttccaac atgaaaggaa taacaccagc aacatataaa
     1441 tttgtatcaa attctttcac aaaaaagtcc atatactcaa gatattgcat cgcatttctc
     1501 atcgatttta cttgggtctg taagacgacg attgcccaat ccgcaatgta catcgcggat
     1561 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
     1621 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
     1681 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
     1741 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
     1801 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
     1861 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
     1921 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
     1981 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
     2041 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaag
     2101 atatatatgt actaccttat aattaataag taaaaggtag tacataaata aggaaaagct
     2161 gaaatatatt gatttatagc tttcctcaaa gtaatgacgt tatttaattt atcgagaagc
     2221 agagaatact cgcgacttta gtcatgtaga cgaatcagat tttcgataaa aaagaagccg
     2281 tccttcttaa gaacgacaac aaatcactag attaattttt tcaactgttc ataaaagttt
     2341 tcatgcgttc cacataacaa tactggaata accttgtttt ctataatctc gtaagctact
     2401 ctatatgttg tgcccgcata tttgaaccca ctagaccaaa tacctgataa atccccagtt
     2461 ttttgttcac ccttatatgg atcaatcgca atttcatcat aaattaaatt caaaaatttt
     2521 tctttcaatg gcttctcttt tagcttttta aatacttaat gacatgtttt gaaggaacga
     2581 tttcataagc caatttctgt tgccaactcc tctttagtca ttactttaga attagtaagt
     2641 gtatcttcag caatatctct aaaggaagaa tgcattttgt tttttctatt cttaaattct
     2701 acaagaatct catctttatc gtagccttct gcaattatat cagacaaaat atcttcgcta
     2761 aaatcataaa attctttttg tggttcaaca aattcataaa gaataccatt ctctaccaac
     2821 tttgcttcaa cttcgactcc attaggaaca ttaaaatctt taggtactgt caacatgatt
     2881 gaattgcctt gttttcttgt ttttgtaatc atttaaatcc ctcatttcta acagtatgta
     2941 attacttagt aaatacatta taccacgaga taatgtattt acaagtaatt actgtgactc
     3001 gtaatgcgtc cttgctgaat aaatttcaac cacgtgttca tcttcatcta ccttataaac
     3061 aacccggtgt tgtctattca atcttctaga atacctacct tcatgtgtag gttttaactt
     3121 ctcaaatgat tgggtaggga gatagggatc ttcctttaaa gtctgaataa ccttctcaaa
     3181 ttgaggtttc aaattagact gtttgatttt ctttaaatca gctttagccg agtttttaat
     3241 catcacagaa tagttattca ttataagtta tcccaatcaa tttcatcaac attagtaaat
     3301 ccactatcat ctttttctcg cttacgaaca acacccaaag tgcctatttg ctcaagtaat
     3361 agcgtttctt tgattgcttt ccaatcatcc aaaccaataa gcaccgcatt attttcactg
     3421 cgctcactaa tgatttcaat ttccgtatga ttctcattta catctttcaa caacttataa
     3481 aaatttttcc gtgctgagct aggatttaag actgtttttg ccatatctac tccccctcta
     3541 atttatatct ccattctaac gtacttaatt aagtacgtaa attcgattgt tttgtttaaa
     3601 ttggagtacc aaaacagcat tgtaaagtgt tatccgtttc ctttataccc gtggtgctag
     3661 ttaatttttt cctagaggaa aatgtctaaa catgttggta aatcaacaaa aaaacgcata
//
LOCUS       DANKHL010000231         3719 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000231 DANKHL010000000
VERSION     DANKHL010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.242"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(93..1214)
                     /gene="mnmA"
                     /locus_tag="PW101_002596"
     CDS             complement(93..1214)
                     /gene="mnmA"
                     /locus_tag="PW101_002596"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDL2514966.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            1618..2217
                     /locus_tag="PW101_002597"
     CDS             1618..2217
                     /locus_tag="PW101_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HDL2514967.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(2355..3641)
                     /gene="efmA"
                     /locus_tag="PW101_002598"
     CDS             complement(2355..3641)
                     /gene="efmA"
                     /locus_tag="PW101_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HDL2514968.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
BASE COUNT         1141 a          819 c          649 g         1110 t
ORIGIN      
        1 aattaatttt attgcaaaaa aacagctatg ctatccaaaa gaaaaggtgt ttcttttgtt
       61 agccatagct gttttgattc atttaatatc gcttaaacgt attgtaaaac ttttgtttct
      121 tgatatgcgt ggtcgatcaa accaccaccc agacattcca ttccatcata aaatacaact
      181 gcttgtccag gagtgattgc acgtacaggt tcgtcgaata cgacttctgc acgattatca
      241 tccaataagc gaactgtcac aggaacgtct tgctgacgat aacggaattt cgctgtacac
      301 ttgaattctt ttggcattgg ttcattcgtc gtaaagtgga tttcacttgc atccaagctt
      361 gtggcataca atgcaggatg atggaaccct tgccccacat ataatgtgtt tgtcgctaga
      421 tcttttccta caacaaacca gggttcttgc gaatctcctc cgccaccgat ccctagtcct
      481 tgacgttgac caatcgtata atacatcaag ccagcatgtt gtcctttgac ttcaccatca
      541 agagtgacca tatttccttt ttttgctggc agatagttgc tcaaaaattg tttgaagttc
      601 ttttcaccaa taaagcagat tcccgttgaa tcttttttct tagcagtcgc aaggcctgcg
      661 cgttcagcaa ttgcccgtac ttctgatttt tccatgccgc ctagagggaa catcgttttt
      721 gaaagctgtt cttgtgataa ttggctaagg aagtaagttt gatctttatt attgtctact
      781 ccacgaagca tgtggaccac gccgttttca tcgcgagtga cttgcgcata atgtccagta
      841 gctacataat cggcgccaag atccattgca taatccaaga atgctttgaa tttgatttct
      901 ttgttgcaca tcacgtctgg gttaggtgta cgtcctgcgc ggtattcggc taggaaatat
      961 tcaaatacac gatcccaata ttctttttca aaattcacag agtaataagg aatcccgatt
     1021 tgagcagcta ctttcgctac atccttataa tcttccgtgg ccgtgcatac accgttttcg
     1081 tccgtgtcat cccaattttt catgaatatt ccgatcacgt cgtatccttg ttcctttaac
     1141 aaaagtgctg ttacagatga gtcgacaccg ccgctcatcc ctacgacaac gcgtgttttg
     1201 ctgttgtctg tcatgatatc accatcattt caattagatt ctgaaacatc tacgagttga
     1261 aggtcgcaac ttttcagtat tttacattat acgtcatatt tctgaaaatt tcatccctta
     1321 attatcaagt cgtaaaaata ctgttctact ggacctattc agtacacaaa catgatagtc
     1381 aaccaaaaaa tcactcatct tttgagcatt cgatataaaa aattggcata ctcataaaaa
     1441 gggccagcct ttcggcaata aggaataatt ttataaattg tgaaaaacta tttttcaaaa
     1501 ttgcttctta tcgcttaaag ctgcacgtct catttacaac cttttctttt tagatgttat
     1561 gagttgctca gataagtgat tttgtttact ctaagaaaaa agggaggcaa aagatacatg
     1621 aattacttta tcagtgatat gcactttttc catgaacgct tattaggaaa aaacgacttt
     1681 gctccgcggc tctttgcaaa tgtggatgaa atgaaccaac agttgatcac tagctggaat
     1741 gcagtaataa aagaaaccga ccatgtttat catctaggcg accttgctat gcatccgcaa
     1801 tatgaaaaag caaatgcaga aattttggaa ctggtcactc aactgcaagg aacaatccac
     1861 ttgatcaaag ggaaccatga cagtcgcgct tttttccgtt atttagaaaa aaatgatcct
     1921 aggcttactg acggaagtcc caaattttat ttttatgatg taggtgccat cgttaaattc
     1981 gaccatcaac aatattactt gactcattat ccgatgttgc tcgggcctaa cgacactatc
     2041 cgcaatcttc atggacatat ccatcattac agcgtaccta tcgagaacga tgtcaatgta
     2101 ggcgtagacg caccggaaag agaactctta aaggaaaaac taccatttgg cactccgtta
     2161 tctgagcaac aaatcaaaga aatctatgaa gcaaagagga agtacgatag gtcgtgaaaa
     2221 aactgcccta tatcaagtat taaacaaata aagataatta ctcatatttt tcatttgagt
     2281 aggcttattg ccacaggaac attaggatca gaaatggagt tgtaacaaga cttctgtcac
     2341 aactccattc catactattc gtctttttgc tcatggtgtt cttttaccag ttttttctgt
     2401 aattctttat cataatttcg ggcagctgac gttaaaaata aaacgattcc gcataataaa
     2461 atcccgattc cggaaaacaa gaacattttt tctaccccta aacgatcagc taatggtcct
     2521 gcaaataata atccaattgg acctgccagg ttcatcaaag agttgaagtt ccctaagaca
     2581 cgacctaaaa cattcgattc ataactctgc tggatcattg ccatcagcag tgtattgaaa
     2641 tatggcgttg cacaacctgc tcctgcattc aagataagaa agtacaagaa cccttgacta
     2701 tcgtttggaa ccaatccgct tgcaccgatc gtcaggccca taatgatgta ggctataata
     2761 ataggtttca ttcggtcttt ccatgttcct ataaaactga tcagtgcacc gcctaagagc
     2821 attccgactg cgtagactac ttccactgct cccgcctgtc caacggaacc accaaagtaa
     2881 tctagcgtca tcaatggata cagactgatt gctggcatga acaacagcat cacaaatgca
     2941 ccattgatcg ttatgtacca taacccttta ttatccatca attgctgcag cccaaatttt
     3001 gcatctttaa aaatagtaag cggaacttcc aatatttcag gagaaacttt tgggatcttc
     3061 acaaaaatca ataagccgac accaaaaact gcccccaata catctaataa aataaggtaa
     3121 ttgacaggaa cgactgaaaa caaagccgca cctagagctg gcgcaatgat gaaattcgct
     3181 gattggacca ttcctaactg tccattcgtc cttgttatgt gagaagatgg aacgattgtc
     3241 ggcataatcg actggatcgt tggcatttgg aatgtttgtg cgaccgaacg cataaacaaa
     3301 gacacaaaaa ctagccaaat cgggaaggat tctgaaatcg ttccgactac tgctaaaata
     3361 agcgcgaaaa tcgctacaat aatatccgtg acaatcaaaa gagctttttt atcccagcga
     3421 tccactagcg gtcctacaaa tgggcttagt aaaaccatgg gaatcattcc tagtaaagta
     3481 gcaaaactca aaattgtcgc cgaaccggtt tctcttgtca aataccagat gatcgcatat
     3541 tggacgacca tactagtgat tcctgacaaa aactgtcccg ataaaaagaa caaataattt
     3601 ctcttccaat tcgttaaaac gaccgactgt tcattttcca taaacatccc tcttttcttt
     3661 ttcattttgt atagcgtttg cacaacaatc attatacggg attctttttt caaaaaata
//
LOCUS       DANKHL010000232         3572 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000232 DANKHL010000000
VERSION     DANKHL010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.243"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(228..1115)
                     /locus_tag="PW101_002599"
     CDS             complement(228..1115)
                     /locus_tag="PW101_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2514969.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1112..1981)
                     /locus_tag="PW101_002600"
     CDS             complement(1112..1981)
                     /locus_tag="PW101_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2514970.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2118..3404)
                     /locus_tag="PW101_002601"
     CDS             complement(2118..3404)
                     /locus_tag="PW101_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2514971.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
BASE COUNT         1095 a          748 c          609 g         1120 t
ORIGIN      
        1 tgacaatcag gacagtcatc ggcaatatag cagcaatcac ttctttaaaa ttttttagca
       61 tgtatcctcc tcctttactc tttaagttta ccttaaaatg aaagagtttt cgagcacaaa
      121 cttaaaacgt tatcatttaa tttttctcaa cataaaataa cacacgtccc aaaagtatct
      181 ttttacttgg aaaaatgact ttgaaggaac gcgcttattg tgttttttta ttttacagag
      241 ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa atacgatggc agttggtaaa
      301 gtagcgatga ctaatccagc catgatcatt ccatagtctg gtgtatagga agcacccata
      361 gcagataaaa caagcggaac cgtccgttta tcaggagatt gcaaagctac taagggccat
      421 aaataattgt tccagctact catgaatgtg atgattgcag ccgcggcata ggtattttta
      481 gccgtcggca tatacactct aaaaaaaata cttagttctt ttagaccgtc taatcgagcg
      541 gcttctacta gatcttttgg aaaagattta acattttgtc gaaagaagaa gatcaaaaaa
      601 gcagtgctaa cagatggcag gatgacaacc aagaaactgt tgattcccaa aaccgttccg
      661 ttcaattgtg aaaacattcg atataaaggg atcatcaatg ccgcaaaagg aaccatcatc
      721 gatagcagta gaatattgaa aattaggtct tttcgtttac tttgataaat ctcaaatcca
      781 tatcctgcca ttgacgaaat cagcaatgca aacacagtgg tcaccaaagc gatcatcgct
      841 gaattccaaa gggaacgagt aaaattcagc tcattggaaa acaaattttg aaaattgatg
      901 atcaagttac tgccaatctt tatttttccc gatgtgatat ccacgggtgt attcgtcatt
      961 cccaaaacca tccagacaaa agggaaaatg gaaataaatg ataggatgct taatgctgta
     1021 tactttaata ctgacccaat tgtcgaacga agttctaatg cttgtttttt gacgttgggg
     1081 cgttcgattg tttctttttc taccatattt ttcatcgact ttctcctcct actttcgttt
     1141 ggattatcga caatatcaca ataaaggaaa caataacaaa ggaaactgct gccgcatatc
     1201 caaaattagg cgtgaattta taactcaaat tatagatgta ttgagatagg gtggtcgttg
     1261 catttgctgg tcccccgcca gtaatatttt gtacttcatc aaaaagctgc aatgtcccga
     1321 tcgtcgaagt aatggaagta aacaaaatga ttggttttaa atttgggatc gtcactttga
     1381 taaactgctg ccatttagaa gcaccgtcaa tagatgctgc ttcataaatc tccgaatcaa
     1441 tattttgcat gcctgaaagg aaaaaaatca tattgtatcc agtccaacgc caagtgatcg
     1501 aaagaatgat cagaactctc gcccaaattg gatgagtcaa ccagccaatc ggtgtgtcaa
     1561 tcataccaat gcccaacaac aagttgttta caaggccaga ttgagaaaat agacttttca
     1621 tgatcagaga ataactgacc atcgaagtga tacatggaag aaagatagcg gttcgaaaca
     1681 accctttata tttcaacttt ttatcattta aaaggttgga aataaaaagt gctagaaaca
     1741 acatgatcgg tacttgtaca agtaaataca ggaatgtatt aaaaaaagct tttttaaaag
     1801 ttgcatctgt caacatccgc tgataattac ctaacccgtt aaatgtcata ttgacagggg
     1861 gaccactttg aaaagacgtg atcaaagcca aaaccatcgg aataaaaacc atgattgata
     1921 tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt gaataaatct ttctgcttca
     1981 ttcttttccc tccaattcta tcattgatta tctacatgaa aagacaagaa agacaatatc
     2041 tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct gatgaaaaag cgaagaaatt
     2101 cactttttta ccaccgatca attagccaat tgtgcttcta cttgtttttg tgtatctgct
     2161 agtaccttgt ctgtctcttc tccattgatg attcgatgaa gagcttcagc tacgactgct
     2221 tcaattgcat aggtctcatg tccgtaattc acttctggta tttcagatgt ccatgttgaa
     2281 aaatcgtcaa agatcttttg accactgtaa aattcagaag cttcttgata agcttcttgt
     2341 tcattcgcgc taagcatcgt agatactagt ccgatttctt tcgcaagagt gcccatcagt
     2401 tcttcattcg aagcaaacgt tgcttctagg aaatctttcg ctgaatcttc tccagctact
     2461 cctttgatca cataccagcc agcgccgcca aggttagaag cttgcgcttt ttgcagattt
     2521 tctggaagtg caggtattgg tgcaatcttc cattttccag attggttttc tgctccttgg
     2581 atcgttgaag aataccaagc gccagttggg ctagaagcta ctgctcctga ttgtatcgca
     2641 gttactccag cattccagtc agaggtttgt tcgactagat cttctttcaa aagagtggca
     2701 aacaattcaa gaccatactt taaagatgca ttgtcttgga tcgtgactgt ctccccatct
     2761 tcagatgtat accattctcc tgcctgttgc ataatcatcc ttactcgacc aaggtcagaa
     2821 ggattgactt cagcgatttt ctttcccgtc ttttctttga catctcgcgc tacttgaata
     2881 tactcgtccc acttcagatt ctccatatcc tcttctgtat aacctgcttc tgccatcaga
     2941 tccgtacgat agaagtttgc tgttacgccc gaatcgaaag gaacaccgta tattttatct
     3001 cctactttgt ttaccgcaaa tttatacggt gcaaaatgat cttcctttac aatagcagac
     3061 aaattagcga atgcatctgg ataagaagtc aaatagcctt gtatcctata atcttcaatc
     3121 aagacgatat tgggcaatcc ttctgtattc ccgctcgcta aagcggtgtt cagtttctga
     3181 acgatatcat cctgagacat cgtaacaact tctatctctg tttcttcgtt ctcataaact
     3241 ttttttgctt cattcactgc tttgatattg aatgtctcat cccacgccca gacagtgact
     3301 ttttcttctt tgtctgctgt actactagca cttccgcatc ctgttaagaa acctgatgct
     3361 gccgctaaac aaacgactcc agcaattaac tttctgatgt tcatttttat ccgctccttt
     3421 tccatccatt agttgtttac gtttgctaca ttcatcatac caaaatttga aaacggtttc
     3481 tttattattc agatacttca aaaatttctc ctaaaataat aaaaaaatac aaatcaacta
     3541 tgacaaaagg aataatctta gcatctagac ca
//
LOCUS       DANKHL010000233         3569 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000233 DANKHL010000000
VERSION     DANKHL010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.244"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            150..1916
                     /locus_tag="PW101_002602"
     CDS             150..1916
                     /locus_tag="PW101_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDL2514972.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            1931..2368
                     /locus_tag="PW101_002603"
     CDS             1931..2368
                     /locus_tag="PW101_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2514973.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            2381..3532
                     /locus_tag="PW101_002604"
     CDS             2381..3532
                     /locus_tag="PW101_002604"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL2514974.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         1195 a          635 c          771 g          968 t
ORIGIN      
        1 ttaaaattga agttttctga taccgaaatc tttttatttt gctgacatac tcaaaccttt
       61 ggcacaaagt ttcgtgccaa agtacgacta tttctctttt tagtaagcgg atacaattag
      121 agtatcaaaa ggagagaggg agagacaaaa tggaacggac gattcccaaa aacaacattt
      181 cgctaaaagc gagggtacaa aaattaggca gtactttatc aagcatggtc atgccaaata
      241 ttggagcact gattgcctgg ggtgtattaa cggctctatt tatcccagat ggttatttac
      301 caaatgaggc tttggctacc atggtaagcc caatgctaac ttatctgatt cctttattga
      361 tcggttatac tggcggaaaa gtaattgctg gtgaccgcgg atcagttgtt ggggcaattg
      421 caacgatggg tgtgattgtc ggaacagata ttccaatgat gctaggcgcg atgatcatgg
      481 gacctcttgg tggatatgcc atcaagaaat ttgatcagct attccaaaaa cggatcaaat
      541 ccggttttga aatgctagtc aataattttt cagcaggtct tatcggtttt ggattagcgt
      601 tattaggttt ttctgcgatt ggtccagttg tcgatgcatt gactcaagca atggcaaaag
      661 gtgtagagat cattttatct gcccatttga ttccattgac aagtattttt attgaaccag
      721 caaaaatctt atttttgaat aatgcaatca atcatgggat tttgacacca ttgggaactg
      781 aacaagtagc agaggctgga aaatctattc tattcttgct tgaagcaaat cctggtccag
      841 gacttggcgt attacttgct tttactttgt ttggcaaagg agcagcaaaa tcttcagcac
      901 caggagccat gatcattcac tttttaggcg ggatccatga aatctatttt ccttacgtta
      961 tgatgaaacc gttattattc ttatcagtta taggcggagg aatgtcagga agtttcattt
     1021 tccaattgat ggatgctgga ctaagagcgc cagcttctcc agggtcgatc attgcgattc
     1081 tagcgatgac accgttcaat tcttaccttc ctgttatttt gggcgtgatt gttgccgcag
     1141 ctgtttcgtt tggtatttct gcagtaattt tgaaagcgga tgcgaaagat acaaatgaag
     1201 acttcgaaaa atctgttcaa gagacaaagg cagctaagca agcttccaaa ggtgcccaag
     1261 gtattcctca aggagctaca ttctctggcg tacaaaaaat catttttgct tgtgatgcag
     1321 gaatgggatc aagtgcgatg ggcgcttcga tcttgagaaa aaaagtacag gaagcagact
     1381 tgcctcaaac ggtgaccaat caagcaatca gccaactgac agatgactcg aatacattga
     1441 tcgttactca ggtcgaacta caagaaagag caaaacaaaa agcaccgagc gcgcgttttg
     1501 tctcagtaga aaactttttg aattcacctc gatatgatga aatcattcaa gaattaagca
     1561 gcaagcagaa aacatcagtt tctgaaattg agaatgagcc agcagaagag tttctttcag
     1621 caaacatcaa acaaatcaaa gagattctac ttgtccacga tgatcgttcc ggatcagcaa
     1681 caatggggat gaccgtgctc aaagatatat tagacaagaa ccatttaact atcccgttaa
     1741 gaaaagtcaa tatccaagat ttaaaagatg aacctgattc actgatcgta gccaaagaaa
     1801 gtttagcgga aaaagtacag atggctgcac ctcaatccat tcatttatca gtcaacagca
     1861 tggtcaatcc gcaaaaatac gaatcgattg cacaatattt gaaacagaca gcttaaggga
     1921 ggaaaacagc atgcaaagtt tgccattcga catgatcgag ctcaatcgat cttttaaaac
     1981 caaagaagaa gcgatccgct attgcggtca gatgcttgta gaagcagggt gcgttgatga
     2041 ggaatatatc gatgccatgg tagatagaga caaaatgtta tctgtttata tggggaattt
     2101 tatcgcgatt cctcatggaa cagatgaagc aaaaaaatat gtgaaaaaat ccggcatctg
     2161 tgtcgttcaa gtaccagatg gtgtagattt tggtaccgaa gaagaagaaa agattgcgac
     2221 tgttttgttc ggtattgcag gagtcggaga tgagcatttg caacttgtac agcaaatcgc
     2281 actttattgc agtgatatgg acaatgttgt ccagctagca gacgcgctca gcaaagaaga
     2341 agtaacggaa aatctagcaa ttgcttaagg agagagaaaa atgagagcag tacattttgg
     2401 tgcaggaaat atcggtcgag gatttattgg agaggttttg gcagctaatg gatttgagat
     2461 tactttcgta gatgtgaatc acacgatcat tgatgcttta aatgaaagaa aagaatacga
     2521 aatcgaatta gcagatgaat caaaagaaaa aatcactgta aaaaatgtca aaggaatcaa
     2581 caatcaaaca aatccagaaa aagtaatcca agaaatcgct gaagcagatt tagttactac
     2641 cgccattggc ccgaatatct taccttttat tgctgagttg attgcaaaag gtatccaaga
     2701 aagagaaaag gaaggaagca caacatcttt agatatcatt gcctgtgaaa atatgattgg
     2761 cggttcagca tttctagaaa aagaagtgta taaatatctt ccggaaacat catttactga
     2821 caaatatatc ggctttccaa atgcagcagt cgatcgaatc gtcccattac aacaccatga
     2881 agatccttta ttcgtccaag tggaaccttt caaagaatgg gtcattgacg atagccaaag
     2941 aaaaaataaa aaaatccaat taaaaggggt tttatatgta gatgatctag aaccatatat
     3001 cgaacgcaaa ctatttagtg tcaatactgg tcatgcgact gtcgcttata caggtgcctt
     3061 gttaggttac cagaccattg atgaagcaat gcaagatgcg ttagttgtgg cacaattaaa
     3121 atcagttttg caggaaacag gaagtttgct gattgccaaa tgggggtttg atgcagagca
     3181 gcatcatgcg tatattgaaa aaatcattca tcgcttccaa aacaaaaata tctctgatgc
     3241 gatcacacgt gtcgctcgga caccattacg taagttaggt tatcaagaaa gatttacacg
     3301 tccagtgaga gaactacaag aacataactt gacttgccca catttgacgg caacaatggg
     3361 gattattttt aattactatg atcctgaaga tgaacaaagc cgtcaattgc atgaaatgaa
     3421 gatccatgaa aacttggaac aattgattca agaagtcaca ggaatcaatg atccgaaaac
     3481 aatcgggaat atcaagcaaa acgtgaaccg ttatgccaag caagtcgctt aaaataggat
     3541 acgaaaaagg ggaatgtgac gaaattttt
//
LOCUS       DANKHL010000234         3499 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000234 DANKHL010000000
VERSION     DANKHL010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.245"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            51..932
                     /locus_tag="PW101_002605"
     CDS             51..932
                     /locus_tag="PW101_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2514975.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            945..2555
                     /locus_tag="PW101_002606"
     CDS             945..2555
                     /locus_tag="PW101_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HDL2514976.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2632..3291)
                     /locus_tag="PW101_002607"
     CDS             complement(2632..3291)
                     /locus_tag="PW101_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2514977.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
BASE COUNT          957 a          679 c          698 g         1165 t
ORIGIN      
        1 ctatttaatc ttgtttagta tatattttta gaaaaaggag tggggttttt atggcagaaa
       61 tcgtcaaagt cacacatttg aaaaagagct ttggtcgttt cgaagcttta aaagatgtct
      121 ctttcaccgt acattcaggt gaagtcgttg gatttatcgg accaaatggt tctggaaaat
      181 ccacgacgat tcggacactt ttagggatta taaaaaaaga cggcggaacg gcaacgattt
      241 ttgggcaaga tgtatgggaa gatagtttat ctatccatga acgtctttct tatgttccag
      301 gcgatatctc tttgtgggga aatttgactg gaggagcgat catcgatctc ttcatgaagc
      361 ttcatggaaa agggagtgct caaaaaaaag atgaactgat tcaacgtttc tctcttgatc
      421 ctaagaaaaa agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc ttgattgctg
      481 ctttggctgt tgattcggat ctctatttat ttgatgagcc tacttctggt ttagatcctt
      541 tgatggaaac gattttccaa gaagaagttg aaaaattgaa agaacagggc aaatccatcc
      601 tactttcttc tcatattctc agtgaagtcg agcgactagc ggataaagtc gtgatcatac
      661 gcgaaggaac actcgatgaa gaaggaacac tcgatgaatt gcgtcatttg actcgttcga
      721 ccatcactat agaaacagaa gcagagaata gtcacttagc acaattgaac ggtgtccatg
      781 actttgtgca aaaaggtcac ctcgctcaat ttgctgctga taacgaagct ttcgatgtga
      841 ttttagctga agcgtccaaa ttaggtgtga aaaaatttga atctattcca ccgactcttg
      901 aggatttatt catgagacat tatgaaggat gaggaggaag cgaaatgact gataaatttt
      961 cacgctggtc cacccttttt tctcaaaacc ttagccgtga ctggaaaaag atcctgatat
     1021 ggctagtcgg tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa gaattgactt
     1081 ccggacaagg attagctgga ttatacgaaa ccttgcaaaa cccggcaatg attgcaatgg
     1141 tcggaccaac tccagcttcg aatgctgctg agtatacttt aggagcgatg tatgcaaatg
     1201 aaatgttgct tttttgcagt ttgtttgctg ctgtgatctc tgttttacac gtggtgagcc
     1261 aaacgagaaa aaaagaagaa cttggtctga ttgaacttat tcgttccttt cgagtcggac
     1321 gtcaagcaaa ctctttagct gtcatcgctg aaagtattct gatcaatttg cttttagcaa
     1381 tatcgatcag ctgtacgatg atttttttcc aagcggattc gatcactgca gaaggtgctt
     1441 ggctttttgg gctatctgta ggtagtatgg ggattttggg gagtgccgtt gcactgtttg
     1501 ctgcacaact gatgccaagt tcagccagtt cgaatggtct gtctttagcg tttttaggtg
     1561 gactttacct tctacgagca ataacagatg tagctgattc ctccctttcg atgtggaatc
     1621 ctttaggctg gtcttatttg acctatccat ttacaactaa caactggatt cctttgcttt
     1681 atttgttttt gttcagtcta atcttgataa tcagttcatt tgttttagag gaacatcgag
     1741 atttgaacac cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa cgttctcttt
     1801 tatccttgcg tggtttatta ttctatctaa ataaagggat gacaatcagt tggcttatca
     1861 cttttggttt gatgggggcg gcatacggct ctatctacgg gaatatgcaa cagtttttag
     1921 aaagtaacga cttaatgaaa cagatgttta tccaatctgg cgtctcaatt gaaaaaaact
     1981 ttacaagcac gatcatggta gtcttgattt gtctagctgc tattttacca attgctagca
     2041 tcaatcgttt atttacagaa gaaacccacg gacgtatgaa tcagctgttc gcaacaaaaa
     2101 tttctaggaa ccaactttat tggaccaata tgttactagc tattctattt gctatattgg
     2161 caatcctgat tagttctggt gcactagggc tttctgcttt ggcttcgatg aaggatgcga
     2221 aagacatgga tctattgatg tttcttaaag ccgggttcaa tttcctgcct gccgttttag
     2281 tgtttatcgg aattagtggc tttctattcg gatggcttcc tcgaatcggt aaacttgctt
     2341 atatctactt agcttactcc tttgcactca attactttgg cggaattttg gatttaccag
     2401 aatggttttc caaaacagct gtccctagct gggttccacg gatgccaaac gattcttttg
     2461 atctttccgt gttccttacg ttatcatgta tcagccttgc actgttcgtc cttggttcta
     2521 ttggttatag gcgaagagac ttactagaag gataataaaa aagcatgtac aatttcatgc
     2581 tagcctccaa atctgggtca aaaatccaac atttggaggt cattttttta tttacaagtc
     2641 ttctaactgc tctttatcaa tgatccagac ttctcgattc tttcttttta cataaccatt
     2701 ctcctctaat aatttaaatt ttctagataa agtttcgggg gtcgtaccga taaatgcagc
     2761 aagttctttc attttcattg gcaggaaaaa gtgatccttc cctgtcgctt tagctaagtc
     2821 aagcagatag acagcgactc gttcttctac tttttccatc atcaaaaaag ttgtctgctg
     2881 ctgtactttt gttaatttct caacgttgat tgctaaaagt ttcagactaa gggcgggata
     2941 atgcagtaag agttccgaga aatctttttg tctcaatatg caaatttccg ttttttctag
     3001 tgcttcgcca aataaagact catttggaac gcctagcaac tggctctctc cctcatatcc
     3061 gcccggctct attactcgta aaagctgttc ctttccgcta gcagataatt gatacacttt
     3121 catactgccg gaggccacga tcactaattg aggttctcca ttcggcgcaa gaatctgttc
     3181 tcctttttcc acatagtgat gtttgacaag tgcgtgtact ttttcttgat cttgctgtac
     3241 taagtgattg aacaaaggaa caaatgaaac acataaatga tcatgactca taatctctcc
     3301 tccttctaaa cagctatttc taataaagaa acataacttc ccttattcca actgataatt
     3361 ttcttttatc atagcataaa cgtcaagcga accagatacc agcaaataga gtggatttct
     3421 ttttgtccct tgctttattt tccttctttt gagaccttaa gtaattgatt cctatcaatt
     3481 ttttaaatat ttacttttt
//
LOCUS       DANKHL010000235         3482 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000235 DANKHL010000000
VERSION     DANKHL010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.246"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            48..1787
                     /gene="yfcC"
                     /locus_tag="PW101_002608"
     CDS             48..1787
                     /gene="yfcC"
                     /locus_tag="PW101_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDL2514978.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1944..3128
                     /gene="iadA"
                     /locus_tag="PW101_002609"
     CDS             1944..3128
                     /gene="iadA"
                     /locus_tag="PW101_002609"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDL2514979.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3239..3442
                     /locus_tag="PW101_002610"
     CDS             3239..3442
                     /locus_tag="PW101_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL2514980.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
BASE COUNT         1108 a          585 c          814 g          975 t
ORIGIN      
        1 attttttatt catcaaaatt actgaaaaaa gaaaggaaga aataaatttg aagaaaacaa
       61 attcttcagg aaaaccgaaa cgcgacttca ctaaattatc gacaccccat acgtatgtca
      121 ttatttttgg tgtagtcatc tttgcatgga tactgacttt tgtcgttcca gcaggaaagt
      181 tcagcacgca agatattgaa tacaaagatg caaatggcga aacaagtact cgtactgttt
      241 tacgtcaaga ttccttccgt tatgcttacg agctggataa gtcgtatgtg tttgatcaat
      301 tagaagaact gcaagatcat ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat
      361 tggaaaaagt cattgctgac ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct
      421 ccttgacaga tgatgtgctt tacgatcagt acggcgaaaa tatctatgat acttctaaaa
      481 aattgcataa aacagccaaa atatggggaa cagatgattt tggcggcttt ggtttcttaa
      541 attttgtttt tgaaggacta gtatctggag ataagtatgg atctgctgta gggatcgctg
      601 cattgatcct tgttgttgga ggtgcttttg gtattattat gcgtaccgga gcgatcgacg
      661 caggtatcta tgcgtttatc agtaaaacta agggactgga gcgtttagct ttaccgctat
      721 tattctttgc tttttcattc ggtggcgcta cattcgggat ggcagaagag gtaattccat
      781 tctcaatggt gatggttcca tttgtcattg cgttaggata tgactcgatc gtagcagtaa
      841 ccgtgactta tgtcgcatct caagtcggaa atgcgacctc ttggatgagc ccgtttagcg
      901 tagcggtggc tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga
      961 ttatgtgggt agttgttact gcattagccg ctggttatat gatgatatat gcagaaaaaa
     1021 tccgtaaaaa accagaaagt tcgttgactt acaaatcaga tgattatttc cgttctcata
     1081 tcaaaaagac atctgatgaa aataaaaaat tcatgcttgg gcataaattg atcttattag
     1141 agatgttagt tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc tattacattc
     1201 cagaaatcgc ttctcaattc tttgtgatgg gattagttgc tggaatcatt gctgttcttt
     1261 tcaaattaga tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg
     1321 atttagctgg tacagcaatc gtagtaggta tggccaaagg gattcttctt gtcttgggag
     1381 gatctgatgc atctgttcct tcagctttga atacgatttt gcatgggatc gggacggcgt
     1441 tgaccggtgt tccagctgtc attggtgctt gggcgatgta catcttccaa agtttgttca
     1501 acttggttgt tacatcaaat tcaggacaag cagctttgac tatgccaatc atggctccac
     1561 tggcagattt agtaggtgta tccagacaag tagcagttct tgcttatcag cttggttcag
     1621 gttttatgga cgcttttaca cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac
     1681 ggatcgaatg ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc ctgtttgttt
     1741 taggaactat tttcatcatg attgccattg caataggtct gcagtagtac tggagacctg
     1801 taaataaaaa agtaatccaa acagtagata aggactccaa gaaccgtcat tttacggtaa
     1861 aatcaaagga gtaaagatcc tatcgtttgg attatttttt aagatcctat catcagttat
     1921 acttaaagaa agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag
     1981 attacttagg aaaaatggat gtactgattg ccggtgggaa gattttggct gtagaagaac
     2041 agctagaagg cggctacgaa ggagtggaag tagaagaact ttcaggtgag ggaaaagtac
     2101 taaccccggg tttcattgac tgtcatttcc atattttagg tggaggagga gaaggcggtt
     2161 atcaaaatcg aacgccagaa gtcacattga gccaattgac tacagctggt gtaacgacag
     2221 ttgtcggttg tttgggaaca gacggagaag gtagagatat gactgcgttg atcagcaaag
     2281 cgaaaggtct ggaagcagaa ggaatctcca cttatgtata cgaaggttcg taccgattgc
     2341 cggtaaaaac agtaacagat tctatcatca aagacttttt gacaattgat aaaattatcg
     2401 gaatcggaga aatcgcagta tccgatcacc gttcttctca gccaagtttt gaagagttcg
     2461 ctcgtgcagt agcagatgct cgtgtaggag gcatgttatc cggaaaagct gggatcatca
     2521 atgtccactt aggcggagga aaaagaaagc tagagctatt gacacgaatc gtagaagaaa
     2581 cagaaattcc gatcacacaa tttttgccta ctcatgcaaa tcgcacacca gaattatttg
     2641 aagcttgcgt ggcatttgcc aaacgtggtg gcacgattga cttcactgct agcgaagatc
     2701 ctgatttttg ggagaagaca gatggtgaag ttcgcttcag caaagcactg aaacgattga
     2761 tcgaggaaga tgtttcttta gataatttca cgatgacctc agatggacaa ggaagcttgc
     2821 cttattttga tgaaaacaat catttcctag ggttaggagt aggaagtgcc aaggcattgc
     2881 tagtagggat caaagaagct gtgcaaaaag aaagtatccc tttggaaatc gccttacgag
     2941 ctataaccag taatccagca cgtatattga aattggataa aaaaggaaaa atcgaaattg
     3001 gtgctgatgc agatctgtgt atcttggata aagaaacgct tgatattgat acagttatcg
     3061 ctaaaggaga aataatggtc caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa
     3121 gtttttaatc aacagctcta actatctttt ttagaaaacg agcgttctgt ttgtctgtat
     3181 ctgttgttga tgttaccctg aaggtagatc aataaacaaa ctaagaggag gaagcacaat
     3241 ggcagattta aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga
     3301 agcatacggc aaagcaacag acgacaagag caaagaaaca gaagggaaag cacaatcact
     3361 aggtgctgac atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag acaagttttc
     3421 tcgaaaagac gaaaaaaaat aaaaaccact tattactaat tgaatcaagc aaataaaaaa
     3481 tc
//
LOCUS       DANKHL010000236         3465 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000236 DANKHL010000000
VERSION     DANKHL010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.247"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..2232)
                     /locus_tag="PW101_002611"
     CDS             complement(<1..2232)
                     /locus_tag="PW101_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514981.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="PW101_002612"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="PW101_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          971 a          767 c          549 g         1178 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtc
//
LOCUS       DANKHL010000237         3459 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000237 DANKHL010000000
VERSION     DANKHL010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.248"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(229..714)
                     /locus_tag="PW101_002613"
     CDS             complement(229..714)
                     /locus_tag="PW101_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDL2514982.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    804..1149
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1194..1835
                     /locus_tag="PW101_002614"
     CDS             1194..1835
                     /locus_tag="PW101_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2514983.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1854..2489
                     /locus_tag="PW101_002615"
     CDS             1854..2489
                     /locus_tag="PW101_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2514984.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2507..3322
                     /locus_tag="PW101_002616"
     CDS             2507..3322
                     /locus_tag="PW101_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2514985.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         1127 a          569 c          739 g         1024 t
ORIGIN      
        1 aataaaattc cgatttataa tcgaaaaaag aagcattttt atatatgctt cttttttcgt
       61 agaatcaata aatatggatt tggctaacta tggaagaata ctcgtattag ttttcagcgt
      121 tcatactagt tgttacattg tttgattggc caaaagaatt tctgatatat tttgtgctaa
      181 ataaagaaaa agacgcttga attgggaaat aagaaaacta ctctatcatt acaaattttc
      241 ataaactggc agatgttcta agtaaacaag tatcttttca aagtcggaaa aatccataat
      301 caacgtagtg gtattgatat ttggatgaaa gcctacggta tcttgacgat caatagcagc
      361 atcgatcacg acttggattt ttttctcttt gtcgtgcatc aatgctaatg gagtcactgt
      421 acctgctgga acgcctagga ggtcaggaag ctgttcttca gaaacaaaag ataagcgctt
      481 ttcttttaat tgttcagcta gttgttttaa atcggcgtgc ttttcatcag gcaagatgac
      541 aagatagatt tgttttcctt ttcttgattt caataataga ttttttactt gcggaccagg
      601 tagggaaaaa gggacatttt tgactgacgt aatcgcagga tgatcgactt tttgaaaaga
      661 gatacccagt ttttttagta gttcataagc ttcttgttcg gttgcacgac tcatcgcgca
      721 tcaccctcct ttttttatta gtgtatcata aaaaagagtt gacaaatgaa aaataatcat
      781 gtaaattaca cgtaaagtta aaacaaatga catcgaaaag aagagtagcc gttaggtgtt
      841 tttagagagt ctctggttgg tggaaagagg cagcagttaa ccgtgaaaca catcttggag
      901 tcgattttct gaagcgtata gtaggaaaat ccggggagcc cgttacagct gcggtcgttg
      961 ctagatgacc agataaggct ggcttttccg tgaggagtcg gtaaaaaaag gtggaaccac
     1021 gtgattaacg tcctttgatc ttcggatcaa aggacttttt gttttcatct gaacggcaac
     1081 gactgtgtga ggttttttct gcaaggaaaa aataaacaga ggtggaacca cgtataatac
     1141 gtcctcttgg catttgccaa gaggattttt ttattttaaa ggaggaaaag aagatggagt
     1201 atttgattga agttatgccg caattattag taggagcact cacaacattg aaattatttt
     1261 tatttacctt gattggctca ttaccattag ggattgtttt agcattagga ttgtctttgc
     1321 attttaagcc gctacgctac ttgcttcaaa catatgtatg ggttatgcgg ggaacaccgc
     1381 tgttgttgca actgattttt attttttatg ggttgccgac gattgggatc gtttttgatc
     1441 gtttcgaagc agcactcatt gcctttatct taaactatgc agcttatttt gcagagatct
     1501 ttcgtggagg gatacaatcg attcctcaag gtcaatatga agcagcgcaa gttttgcaat
     1561 taagtccctt tcaaacggtg acgaaaatcg tcatcccaca agtgacaaag attgttttac
     1621 cttctgtagg gaatgaagtt atcaacttgg tcaaagattc ttccttgatc tatatattag
     1681 gtttaggtga cgtaatgcgt gctgggaaaa tcgcgatgga acgcgacgcg actttactgc
     1741 ctttgctagg cgtggcaatc atctatctta cattgactgg tattctaaca atcgtaatga
     1801 aacaagttga aggaaaatta aattattacc gctaactgat aggaggaaga aatatgattg
     1861 aattgaagaa agtgaacaaa gcatttggtg aaaaaaaaat aatcaaagat ttgaatctaa
     1921 tcattccaga tgggaaaaca ttagctatcg taggtccttc cggtggcggt aaaaccacgc
     1981 ttttacgtat cttggctggc ttagaaagtt ctgacagcgg agaattttat ttagacggtg
     2041 taccgttcaa cccattcgag cttaaaatga aagaacaagt tgtaggagtc gtattccaag
     2101 acttccagct atttccacat ctgaatattt ttgaaaacat taccttagca ccaaaaatgg
     2161 tattgaaaca gacaaaagaa gaatacacca aaaaagcaga agaattagcg gcttcattag
     2221 ggattagtga tttattgaat atctatcctt atcagctttc tggcggacaa aaacagcggg
     2281 tagcgattgc cagagcattg gctatgaatc cacgggtatt ggcttatgat gaaccaacaa
     2341 gtgctttaga tcctgaatta cgccagcaag tcgagcgttt gatcctatca ttgaaagaag
     2401 atggggtaac ccaaattatt gtcacccatg atttagtatt tgcagaaaat gtcggtgatc
     2461 aattactaaa agtttcccca attcattaag aaaggagaag atagagatga aaaaaaccag
     2521 ttttctattc gctttgatcg caggtcttct tctttttgca ggctgttcga acaaaaaaac
     2581 ttcagcagat aattgggcga ctatcaacga agagaaaaaa atcgttatag gtttagacga
     2641 tacatttgtt cctatgggat ttagagataa agacgggaag ctcacaggat ttgatattga
     2701 tttagccaaa gcagtctttg aacaatatgg gattacggta gactttcagc ccattgactg
     2761 gtcaatgaag gaatttgaac tgaacaatgg aacgatcgat ttgatatgga acggctattc
     2821 caaaacgccg gcaagagaaa agaaagtgca atttacaaaa ccttatatgg aaaacgatca
     2881 agtgctgatc acacctaaag aatcaggaat cacaaacttt ggacaaatga ataataagag
     2941 attaggtgcg caaaatgggt catctggtta tgatgtattt acgaaacaac cagaagtatt
     3001 aaaagagata gtcaaaaacc aagatgctgt tttgtatgat agttttaatg aagcattgat
     3061 cgatctgaaa agcagacggg tcgatggtct gctgatggat aaagtatacg cagactatta
     3121 tttgaaacaa agaaacgaac tagaagactt caacataaca aaaggttctt atgagagtga
     3181 ggattttgca gttggtgcac gcaagtcaga tagtacatta gtgaaaaaaa tcaatgaagc
     3241 cctagatcaa ctagagaaaa caggtgaatt ccaaaaaata tccgataaat ggtttggcga
     3301 agatgttacg ccgagaaatt aacaaatagg ggactgtgac aaaagacgtg tcacagtccc
     3361 tttttccgaa taaacagtgt tctcctgcca actccttgtg actcggagct gaatggcagg
     3421 ctcacaacct cttggtaaac ggtgttcaaa agccagttt
//
LOCUS       DANKHL010000238         3380 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000238 DANKHL010000000
VERSION     DANKHL010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.249"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..1006
                     /locus_tag="PW101_002617"
     CDS             <1..1006
                     /locus_tag="PW101_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2514986.1"
                     /translation="WYAYLVAKELELDLIHTHTEFGAGILGKMVAKKLKIPLIHTYHT
                     MYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVIDTLRSYGVIAPLRVIP
                     TGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYEKNIQALINGFPQVIS
                     AYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDVALYYKAADYFVSAST
                     SETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDEDFAPALIDYISSEVP
                     KDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEEDSIERIKIKLNSFKR
                     "
     gene            1095..1982
                     /locus_tag="PW101_002618"
     CDS             1095..1982
                     /locus_tag="PW101_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL2514987.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            2068..2298
                     /locus_tag="PW101_002619"
     CDS             2068..2298
                     /locus_tag="PW101_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HDL2514988.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(2355..3320)
                     /locus_tag="PW101_002620"
     CDS             complement(2355..3320)
                     /locus_tag="PW101_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL2514989.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         1128 a          562 c          678 g         1012 t
ORIGIN      
        1 gtggtatgct tatttggttg ccaaagaact agaactagat cttattcata cccatacaga
       61 attcggagca gggatacttg ggaagatggt agctaagaag ttgaaaatac ctctgatcca
      121 tacttaccat acgatgtatg aagactacct tcattatatc ggtaaaggaa aagtgatcag
      181 gcaatcccat gtaaaatact tgtctagatt atttgcaaat catacgacag gtgtagtttg
      241 tccaagtgaa cgtgttattg atacattacg cagctatggt gtaatagcac cgcttcgcgt
      301 gattcctact ggtatcgaca ttgaaaaatt caaacgttct gatatcactc agcaggatat
      361 cagcaaatta agggagtcac taggtcttca ggaaaaccat ttgatgattc tttctttaag
      421 caggatttct tatgagaaaa atatccaagc cttgattaat gggtttcctc aggtcatctc
      481 cgcttatcct aacgccagat tagttatcgt aggaaaagga ccttatgtag aagagttaga
      541 ggaattgatc tcagaactgc agttagaaga atttgtgcag ttcacaggag aagtggacaa
      601 caatgatgta gcactttatt ataaagcggc agattatttt gtcagtgctt ctacttcaga
      661 aacacaaggg cttacttata cagaagcgat ggcagccgga acaccttgtg tcgtggaagg
      721 aaatgcttat ttgaaccgat tgttcgatca tccttcatta gggaaaactt ttgaaaccga
      781 cgaagacttt gcaccagcac ttattgatta tatttccagt gaagttccta aagatccaaa
      841 agttttagaa gctaaacttt atgatatttc atctacacat ttcggcgaag ctatgatcga
      901 attttatcaa gacatggttg tttatcatga agaagagatg cgaagaaaag aagaagaaga
      961 ctcgattgaa cgaatcaaaa taaaattaaa ttcatttaaa agataaaatt caaacatttt
     1021 ttatgaaaaa atccttgttt ctagcgtata ctagcataga agcaaggatt tttgattttt
     1081 aggaggtcga aaaaatgaaa cttggtttta tcggaacagg cgtcatggga agtgctgtcg
     1141 cacgccatct actagaagca ggacacgaag tagctgtcta taatcggaca aaagcaaaag
     1201 ctgatccttt agtaacagaa ggagctatct gggcagatac accaaaagcg gtagcagaac
     1261 aaagtaatat tctctttaca atggtaggtt accccaaaga tgtagaagaa atctactatg
     1321 gacagtcggg gattttttct gcagatatct caggacatat tttagttgat ttgactacaa
     1381 gtacaccaag cctggcagaa aaaattgcaa aaacagcaaa agaaaaaggc gcagatgcgt
     1441 tagatgcacc ggtatccggt ggtgacttag gagcaaagaa tggtacactg acgatcatgg
     1501 tcggtggaga agaagccgtt tatgatcaag tactgccttt attcaaagaa tttggtacga
     1561 catttacatt gcatggttct gctgggaaag gtcagcatac aaaaatggct aatcagatca
     1621 tgattgcagg tacgatgaca ggaatgacgg aaatgcttgt ctatgcacaa aaaaacggac
     1681 ttgatttaaa gaaagtgatt gagacgcttt caggtggaag tgcagctaac tggtctttaa
     1741 gcaattattc tcctcgtatt ttaaaagaag attacacacc tggttttttt gtcaaacatt
     1801 ttatcaaaga tttaaagatt gcattagaag aagcagaaaa aatggatctt gtattgccag
     1861 caactaccca agcattgaaa ctatatgaag agttggcaga taaaggtttt gagaatgacg
     1921 gaacccaagc tttgatcaaa ctttggtggc ctgaaggaaa aataccagaa aaaaaatcat
     1981 aaaaaacgag tttttttaga gaaaatcaat gcaagtaggc tatgcttttg atacaatata
     2041 acagaaataa gaaaaggagg acctccgatg acacattcac aattagtcgc aatcataaaa
     2101 cgactggagg ctatggtcga gtcagcagat aatgaactgc aagttcgccg ttttgaaaaa
     2161 gaaggcgtag agaagtgtac agtaacttat gataaagcga ccgatacatt tgaattaact
     2221 gaaacagata ctcatcagca atacgaattt gataatatcg atatcgtagc gatggaaatc
     2281 tatgacttaa tccaataaat tttaggataa ttgacatttg aagttgaaga tgcctaaaag
     2341 aagccgagaa gcaactaatt tagttgcttt tcagcttctt ttttgtttaa cagttcaatt
     2401 tcaaactgtt ctttttctaa ttttttgcgg taactgatca agatttgtac aggatcgatt
     2461 tcttcttttg acaattgacg tttcaaagat aaatgagctt ctttcttttc ataatagaag
     2521 tcaggagaaa cgtcaccaga ggtaatcatt gtttctgcaa gttgaccata acaaacgagg
     2581 tagtgctttt tctgactctc atctgctgct aatggcatgc tgaaggtgag cgatggtgtt
     2641 cttaaaaact gcttttgttc ctggatccgt ctaataaaat aagtaactaa ttcgatatct
     2701 ttttgaagca taccggcttt ttcgaaattc aactgataga tgaaatgttg ttgtcttttt
     2761 ttcaagtaag tcagtagctt tttatttcgg ccttgaaaca gcccttggag ttcctttttt
     2821 ttctgttcaa aagtcatttc ctgtaggtca ggaagctgga ctcgcaaagc taacaaacta
     2881 atttcagaca accaaggcat ttgataagtt tcttccatta tacgcaaaat agacggcatt
     2941 tttttataga gacgaaaagg cccgtatgtt cgagccgtag gagtatctgt taatacaaat
     3001 cccttattgg taatatgtac ataattataa ttactgaaat aattcatttg tcggttataa
     3061 aaaggacggt aatgttggat caattgacac tcgagcaata aagcatctaa ttccgtgtct
     3121 actacgacga aatcaaaatc tgttatatga tgaatcatac gtaatacttt ttttgaatgc
     3181 tgtttattct gatgaaaata gctgcttact ctatttttga gccgttttgc tttaccaaca
     3241 tagatgacct taccatctcg gtctttcata agatagacac ccggcagcat ggggagagtt
     3301 ttcatttttt cttttaacat aggatctcct tttacgcttt gaataaaaag actataacaa
     3361 attttgaaat aatggacaag
//
LOCUS       DANKHL010000239         3341 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000239 DANKHL010000000
VERSION     DANKHL010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.250"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            81..1781
                     /locus_tag="PW101_002621"
     CDS             81..1781
                     /locus_tag="PW101_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDL2514990.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            1751..1969
                     /locus_tag="PW101_002622"
     CDS             1751..1969
                     /locus_tag="PW101_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HDL2514991.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            1989..2879
                     /locus_tag="PW101_002623"
     CDS             1989..2879
                     /locus_tag="PW101_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDL2514992.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            2897..3181
                     /locus_tag="PW101_002624"
     CDS             2897..3181
                     /locus_tag="PW101_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514993.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         1187 a          550 c          712 g          892 t
ORIGIN      
        1 tatggaatct acaaagtcga atacgagact catgaccttt ttatgacgta ctggtatact
       61 tgaagaaaaa ggagagcgcg atggagataa aaaaagttta cttttcacag aaaatgtcgc
      121 tgtattcgcc tatccagcaa gcaaaacaac cccttccatt ttttcaaagt catcaagaaa
      181 gtagagacgc tttttggcgt tctgcaaaaa agcaatgtgc ttttaaagca gtatcgaaaa
      241 aatataccgt ctattttact attgctaaaa gtagaggaga tgttattttt aataatctgt
      301 tgattgaagg tgaaactttt gaatggaaaa aattttttcg gtatatggaa gcttgcgctc
      361 gtttttttat taaagaaaac tgttgttttt tatttcaatc tcctctatca gaagaatggt
      421 tgcgtatttt tcataaaaac ggctatcaag gaaccacaca attaaataaa aagctggtct
      481 atcatacagc acttgtttta ggtggaggag gagcgcacgg tgcttatcag attggtgtgt
      541 ggcaggcgct gaaggaacat gatatcaact ttgagattat cacaggtacc tcggtaggtg
      601 cgttgaacgg cgcattgatt ttacaaggag atatgaaaaa agctgtgaat ttatggaaaa
      661 agctgtcgac aagacaagtg cttgcgttac cagaaatggc tgcagttgaa gaccttcgtg
      721 aacgcttcta tcgagaaaga cgccaaatga cgaaaacagc actaattgaa ggcggggttt
      781 cttctgctcc attggaaaag atggtcaaag acaatttgga tttgtcctta ctcaaacata
      841 atcccaaaat acgtttatat acggtgtcta ctaaactgcc agaactagcg gaagtagtga
      901 ctgatatcca acagacaaaa acagaagaaa taccagattg gatactcgcc tctgcttcat
      961 tttatcccgc tatggcttat cgaaagatcg gaaaaaataa atatgcagac ggtgggtatc
     1021 gcaacaaaat acctattgat attgcgatta acaaaggagc aacagaggca tttgtcgtag
     1081 atgtgcaagg gccaggccca gcgaaaaaaa ttcgtatgcc agacattttt atccattgga
     1141 aatgtcaaac attgtggaca ctaggcagct ttctgctttt tgatagccaa aggaaccaat
     1201 taaatttaca attaggctat ctggaaatga aaaaacgatt aggctattac tatgggaact
     1261 ggtatacatt tcattctgtt aaacctgcaa gaacatactg gcgaggattt ttgtcttact
     1321 taacaaaaga aattcagtta gaattatctt ttttcaaaag cataaaattt tggcaaaaac
     1381 tgcgtaattt atataagaac agggtcgtac cagagacatg cggattggcg atgctagaac
     1441 ttttagcaaa aaaacatttt ttactgccca atgaaatcta tcaagtagac atgatgattc
     1501 aaatgatctg ccaacaagat ataaaaagta tgccggatga tttattggca caaattggtc
     1561 aattaagtac tgaagaatgg cgaaggtatc gaaaatacca gcaaaaaata caaaatgagc
     1621 gcacgaaaac tgtttatttt gattatctgc tacaaggaaa acgcaaggat cgtttgcagc
     1681 atgaattgct taagcaacca gttgacacat tactgatcct atatttatat tatctcaagg
     1741 aggaacaacc atggcacaag aattttcata tgaaatcgta gaagaaatcg ctatattgtc
     1801 agaaaacaac aaggggtggc gtaaagaact gaacctagtc agctggaatg gtcgcccgcc
     1861 aaaattcgat cttcgagatt gggcgccaga ccatgaaaaa atggggaaag gcctgacatt
     1921 gacgaacgaa gagtttgaac aactgcaaaa agcaatagaa aatatgtaat gaggaaggac
     1981 cctgtcgaat gaaaagtatg actggttttg gcaaagccat ccgcgaaaca aaagcgtatc
     2041 agttagatat tgaaataaaa agtgtcaacc agcgttttct agatgttcaa atacgaagtc
     2101 ctaaaatcct gaactttgtt gaaaacgatt ttcgtcagct tattaaacag cagttaagcc
     2161 gcggtcgaat cgaagtattt gtgaatctag cttatattgg caaaaacgaa aaacagatcg
     2221 tcatcaactg ggaattgatt gattcactta tcactcaatt gacagacgga attcaacaac
     2281 gatacggtag tcaaaatcaa gtgtcaatta gtcggatggc ggaacgcttc gcgctggacg
     2341 aaaacttcgt aacggtagaa gaaaaaccgg aagagagcat ggaagaattg gcacagttag
     2401 ctctagatac attgaaaagc gcacttgatt ctattgaggc gagtcgagaa aaagaaggtc
     2461 aagctttagc tttagtgttg aaaaagaata ctgaagaatt caaagaagta ctaaaaaaac
     2521 tcgatagttt tgtagaaatc tatgaaaagg aatttcaaat aaggtatcaa aaaaagctgg
     2581 aggaatttct tggagcgaaa gtcgatgagc aacggttgtt gacggagcta gccatcttgt
     2641 tggaacgcgg agatattcac gaggaactgg atcgtctggc gatccatgta caaaaactag
     2701 atgaacttat cgaagcacaa tgtcctgtag gtcgggaatt agatttcctg atccaagaaa
     2761 tgaatcggga aatcaacacg atcggttcca aatcaaatgc aatcgaaatt aaaaatcaag
     2821 tcgtccagtc taagacgatc ttagaaaaaa tcagagaaca agtacaaaat attgaatgac
     2881 gaaagaggta gagataatga aatattatga cgtaacgttc cacgaattaa gtggaaaaac
     2941 tgtggttaaa cgggatatcc cttctgaaaa aaacggcttt gacgtatgga aagatgcctg
     3001 tgcagactat aatgagaacg aactgtttat tttgatcaac gacggtgctt acgtgacgat
     3061 gaatcggaaa tttattgttc gaattgacac agaagaggta gaagatccga cagaaaaagc
     3121 acgttctcga aaagatgaga tcatgggtgt agtgaatact ttgtccaata tgggctttta
     3181 gcaaggatgc tgaacaaaga aatacaataa ataatcagaa ttggatttct tcgcttagct
     3241 tctttgctct aggaatacat ttaaaggttg tgacaaaatt cgtgtcacaa tcttttttta
     3301 ttttgaataa gtgatgttca aaaaccattt tctcggtatt a
//
LOCUS       DANKHL010000240         3336 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000240 DANKHL010000000
VERSION     DANKHL010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.251"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(510..785)
                     /locus_tag="PW101_002625"
     CDS             complement(510..785)
                     /locus_tag="PW101_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDL2514994.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            892..1656
                     /locus_tag="PW101_002626"
     CDS             892..1656
                     /locus_tag="PW101_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDL2514995.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     NAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            1779..2282
                     /locus_tag="PW101_002627"
     CDS             1779..2282
                     /locus_tag="PW101_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2514996.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            2561..3208
                     /locus_tag="PW101_002628"
     CDS             2561..3208
                     /locus_tag="PW101_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HDL2514997.1"
                     /translation="MEQTIKPYQYFFIKREVDQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
BASE COUNT         1110 a          592 c          679 g          955 t
ORIGIN      
        1 gtgattccaa gtgtttcttt cttatttaaa aaactaaaaa cttttttgtt ctgtctggaa
       61 aatatgggtt aagcaaattc attgcaagtt atagatcagc ttttttacta cctgtcttta
      121 atacttgagg caaagcgcct caaaaacaac cttatcgacg gtgttcaaaa gcttgcatct
      181 acggcattaa gtccagcctg ttgaaaaata tggagagcta tttatcacaa ccagtcctta
      241 atgcttgatg cagaccagct ttttcacaac cttattgacg gtgttttatc agctgcctct
      301 cggtattaag tcattacatt tcaaaatatg agaagctatt ttgaaaatgt ttctccttaa
      361 tactcgaggc aaagcgcttc aaaaacaacc ttatcgacgg tgttcaaaag tcagtttctc
      421 ggtattaagt caaatttatg caaaaatgtg gaaagccatt ttcacaaaat tttccttaat
      481 actcaaaact aagcgacctt ttcacaacct tatcccacta catcaaattt ctttcgttcc
      541 tcaatcatcg gatcttcttg gacatccaca tactgcactc gtccacttgg gctcggagac
      601 ttcttgactt cttcgataaa tttttggatg atgtcgtcat ttccgattgc ttcgattgtg
      661 actgagccgt cgtcttcgtt ttttaccgtt ccggtcacgc ctaactgatc agcaaccatc
      721 ttggtcatat agcgaaatcc cacgccctgg acacgtcctt ggacattcat tttaactttg
      781 cgcatgttcg tcacctttct ttcctgtttt ataaatcaat gataccgatt tttttaggat
      841 gaaacaagct caagcaccta ttccatgata taatcagttg aggtgaggaa tgtgaaagag
      901 atccaatcag caaaaaatcc aagaatcaaa gaattaaaaa aactacataa aaagaaatac
      961 agagaagaaa aggcagaata tcttttggaa gggttccatc tgatcgaaga agctgcgtcg
     1021 tctaatgcag aaataaaaga actttttgtg acacagagag gtatgaaaga atggggagac
     1081 tggctggctg taaacaaaga gatttctact tttctagttt cagatgaggt catgaccgct
     1141 atgtcagatc tacctacacc gcaagggatc ttagcagtga tgacgttaac atctgaagat
     1201 acgccagatt tttcaggagg ctggctttta ctagataatg tacaggatcc tggaaatgtg
     1261 ggaacgatga tccgtacggc agatgcgttt ggtatggctg gtgttttatt aggtaaagga
     1321 acggcagata tttatagtac aaaggtgctg cgaagcatgc agggaagcaa ttatcatttg
     1381 cctgtgttac aaaaagattt ggcagatgta atccctgtaa tgaaaggcaa agggtttacg
     1441 gtgtacggta cagaattaaa tgaagaagca catgcattag acaaagtaga aaaaacagag
     1501 gatttcgcta taatcatggg aaacgaaggt cagggagtaa gtcaggaaat cttgtctttg
     1561 actgatcaga acgtatatat cccaatgaaa ggaaatgcgg aatcattgaa tgtcggagtc
     1621 gcagcaggga tcgttcttta tcatttttct ttatgattaa ggaactatta agctttagct
     1681 attttatcta gagcgactag aataatccga acactggtgg tatacttaga tcaatttcag
     1741 aagaaaatga aattcaaaaa ggtggcagat cgataattat gacagaaaaa tggcgtgaag
     1801 acgaagaata tatgtcttat gtacaagatc ttttagaaac agaagcagtg aaaaaattag
     1861 cgaattacac acagcatgtt cattccactc gtttggagca ttccatcagt gtttcttact
     1921 atagttatct gctcgcaaaa aaatggggtg gcaatgctaa agcaacagcg cgtgctggac
     1981 ttttgcatga tctgttctac tatgattggc gtacgacgaa atttgacgag gggacacatg
     2041 cgtatatcca tccaagaatt gctgtaaaga atgcagaaaa aatcaccgat ttgtcggatc
     2101 ttgagcgaga tatcattttg aagcacatgt ggggtgcaac gatcgcaccg cctaagtata
     2161 aagaaggata tattgtgaca ttcgtggaca aatactgcgc agtcaaagaa gcggctcagc
     2221 ccatgtctgc aagtatgcgt aagagatggc aacgttactt tgggaaagaa tcctcgattt
     2281 aataggtaaa accaataata atagactata ttccgagaaa atctggctga ttttctcggt
     2341 tttttctgtt tgcagttgac tatctgttca agatacggtt tacttttatt tatcgaatga
     2401 atcatgtaca tagaagaatg aacattaaag ggggtgatga taagatgcaa aacgtatctt
     2461 atttaaaaga gtccgggatc ttcaggtaac acatactttg cagaagatca cttttttcta
     2521 caaagtatgc actttggtta gaagaaagga ctttaaaatc atggaacaaa caataaaacc
     2581 ataccaatat ttttttataa aaagagaagt agatcagtta ttaaatgcgt actcatctgt
     2641 caatgatcca aaaacggtac aaacagttca ggcactagca gctgaaaaga ttcgagacat
     2701 attagaacac gaactgccag aaatcgatac atttttaacg acagttttag acgtaaaact
     2761 gacaaaagcg caagcagaaa gagcattgga ggagctgaag caaatcgtcc agccgtttat
     2821 gcagcctagc aaaccacaaa tcgagaaatt attccgaaaa gtgaaaaaac tcaaacaacc
     2881 agcttggaat gaaatcgact tgcaagaaca tacgtacatc ggctggaatg atccaggtac
     2941 gcagaaaaaa ttcatggtct attacgaaga tgaaaaatta aaagggatta gcggtacttt
     3001 atcaccaacg ataaataagg gaatctgtgc aatctgtcag aaaacatcga atgtgtcgct
     3061 ttttctttct acaacgaaag caggttctga tggcacgtat acgaaaaagg ggaattatat
     3121 ttgtcatgat agtgaccaat gtaatcaaca gttaacgaaa ttggctcctt tctatgaatt
     3181 tgctgaacgt gtaaagaaag aacgataatt ttattgtttt ttacagaaaa atatgctatc
     3241 ttttaatgga agtgaatagg tgatggtgtt cgcctttaaa tgctaacgaa agatagctaa
     3301 tgacgcctac taaagtctgg ggatagcccc tttctt
//
LOCUS       DANKHL010000241         3322 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000241 DANKHL010000000
VERSION     DANKHL010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.252"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..149
                     /locus_tag="PW101_002629"
     CDS             <1..149
                     /locus_tag="PW101_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2514998.1"
                     /translation="EYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQKTIKKLNI
                     GGEK"
     gene            146..895
                     /locus_tag="PW101_002630"
     CDS             146..895
                     /locus_tag="PW101_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2514999.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            907..1176
                     /locus_tag="PW101_002631"
     CDS             907..1176
                     /locus_tag="PW101_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515000.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            1182..1346
                     /locus_tag="PW101_002632"
     CDS             1182..1346
                     /locus_tag="PW101_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515001.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1339..1638
                     /locus_tag="PW101_002633"
     CDS             1339..1638
                     /locus_tag="PW101_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515002.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            1638..1940
                     /locus_tag="PW101_002634"
     CDS             1638..1940
                     /locus_tag="PW101_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL2515003.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            1980..2345
                     /locus_tag="PW101_002635"
     CDS             1980..2345
                     /locus_tag="PW101_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515004.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            2835..2975
                     /locus_tag="PW101_002636"
     CDS             2835..2975
                     /locus_tag="PW101_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515005.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            2972..3154
                     /locus_tag="PW101_002637"
     CDS             2972..3154
                     /locus_tag="PW101_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515006.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            3167..>3322
                     /locus_tag="PW101_002638"
     CDS             3167..>3322
                     /locus_tag="PW101_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515007.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIM"
BASE COUNT         1240 a          518 c          723 g          841 t
ORIGIN      
        1 cagagtacac atttgcagca ctctgcagaa tccatcacac agagaagcac aaaataggtg
       61 tgattaattt caaaaataaa tatcaaatca aagggatcaa attaaaccag aaaacaatca
      121 aaaagttaaa tattggaggg gaaaaatgac agaacatcga agttattatg cgattatacc
      181 agccaacgta aggtacgaca aaagacttaa accaaatact aagttgttat acggagagat
      241 aacggccttg tgtaatgaaa gaggcttttg ttgggcaggc aatgagtact ttgcagattt
      301 atatggtgtg aataaagaga ccatatcgcg atgggtaagt gatttgatta agtttggata
      361 cttgaatcgg gaaatcattt acaaagaggg taccaatcaa ataatcaata ggtacctacg
      421 aattaatcaa taccctattg acgaaaaacg caatacccct attgacgaaa aagtcaaaga
      481 taataataca tctattaata atacatttaa taatacaaaa gaatatataa gagagttacc
      541 gccttcgaaa aaatcgaagg ctaagcccgt ccgtcataaa tacggagagt ataaaaatgt
      601 tcttttgtca gatgagcaga tggagaaact caaaacagaa ttccctaatg attaccaaga
      661 gcgaatcgaa cgactgtcag agtattgtga atcatctggt aagacttata aaaactattt
      721 ggcaactatt cgaagttggg caaggaaaga aaaaagtgaa cctaagaacg caagcagtgg
      781 atacaagcgc acaggaagac gagagaagct tcctgaatgg gcaatcgacc aagaagccta
      841 tctcaagaaa aaagcgctag aacgagctaa tagacaatca aaagcaccat tctaagaggt
      901 ggaaaaatga agatcgatta tctagaacta attaatgaaa tagcgaatta taaaaagggc
      961 gaggaattag acgtcctgag agacgtatat gatcaactcg aagaagctgg aattgaagga
     1021 attaagaatg atcgttcgag ttggagtaaa ctcagatact atttcgcact ctatatcgat
     1081 ggaacgcaat taagaaattt agcatatacc aaattactat tcattgattg tgttaaaggc
     1141 ttgcaaaaac atcttaatga acttgagcag gtgtaaacaa gatggaccta aagacattta
     1201 cagcacagat cgaattaatg catcaagaag ctttaagaca aagtgtgtcg tacgaagaca
     1261 agtggctcaa cacatttcat ggcggacgtg agagcgcact tgatcaagta ctcaaattac
     1321 tgaaaggaga atgtcgggat ggataagaaa gcggcaatgc agcgaattat cgaattgact
     1381 tattcagaag attggcagga agataaaaaa atagttgcag aagtccaaaa gctcggtaaa
     1441 tcaatgtgga ctgaaaagcc caaacggaga acgccgagaa agattgcaat ctggcatggt
     1501 gatcgaattc tagtaacggg taccgctaaa cagttatctg aaattactgg tctgagcaaa
     1561 aatattattt gggatagagc taggagctta tggattgatt caaaaggaag acagtttagg
     1621 tatgtggagg agaaataatg gacgaactaa tcgcgaaagt agagcagtgg gcaaaagata
     1681 agggattgga tcaagcggat ttcagcaagc aaatgttaaa aacggtcgaa gaaatcgggg
     1741 aagttgccgc ttctctagct agaaaagatg aacatggttt aagagacgga attggcgatg
     1801 tagtagtaac cttgatcatc ttggcaatgc aaaatgatat ggatttatac gagtgcttgg
     1861 attttgctta tgacgaaatc aaaggtcgga ctggaaagat ggtcaatggt gttttcgtta
     1921 agtccagtga cctgaaataa ttccattaat ggaagaaata actgaatagg aggatagaca
     1981 tgattgatgc aaaacaagtg caaaaacaga aagatggaat gttgatgttt gaagcatatg
     2041 ttctaccatt tcttaatcaa tttgaagttt tggaatgctc tgccagcgga gaagaattgg
     2101 agtatgttgt aattcgtgaa accaaagaaa acgttcagaa gctaaacgaa tttctttgca
     2161 ctattaactg ttgggatatg atagctccag gatttttatg tccagctatg ggagaatttt
     2221 tggaatattg tcgtcttgaa gatgcaggca ctttggattt agcttatctg gtatacaact
     2281 acctaaacat caacacagat catttgtggt ttggcactgc cgaaagaaaa tgggtagtcc
     2341 gctaaccacc aatatcaatg aaaaaaggcg gtttcctgaa agtggaattg ccgccattgc
     2401 tgtaaggttc tttacgcgac tcaaggtcac agaaatataa atgcaaatct cttttgaagt
     2461 gatccaaagc tctcggaaaa tggaagtttt ttgtttcaaa gaataaatga gatttgcgtc
     2521 aagaacctga gagttatttc caaaaaagct tccaaagaac cgaatgagct aaggacatca
     2581 tgtgtaaaca cataatatag ctctcctttc acggctaatc ataacatgaa aaatattcga
     2641 tgtcagtagg aaaaaaggtg aattatgaaa agaatattaa attatcccgg cagtaacaga
     2701 ttctgcaatt aaactccgaa gctgatgtat cagtatcaac tgtagaatat tatgagcgtg
     2761 gagaaagtca agcagtcaca aatgtaaaaa tcgataacgt caacggtctt atctgtttag
     2821 atgaaggcga tagtatgaat gaaattgctg cagaccatga attatcagaa actgctttaa
     2881 ttgatcaaat cgaattagct agacaatact ttgttccata tgcagatacc tggtccaagc
     2941 ataagaatga catcattttt ccagaggaac aatgagagca actgatccag taattatcct
     3001 tgaggaagcc aaatttattt ggactcacga agagatagag caagcacgct tgctcttttc
     3061 tcaaggagtt aagccgagca aagtagctga aataatgggt caaaagattc ttgatgtcgg
     3121 attgcttttg ctccatctag cagaaaaaat ttgatttgag gtgaaaatga tgattcaact
     3181 tgcaggcatc caaacaggga aaatttattt ttctggagaa agcaaaagcg aagccaacca
     3241 atggctattg aaaacttaca cgaacaataa gaagctaagg aaaaaatatc ctgatttgtt
     3301 gctaaaggat gatcagatta tg
//
LOCUS       DANKHL010000242         3199 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000242 DANKHL010000000
VERSION     DANKHL010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.253"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            301..585
                     /locus_tag="PW101_002639"
     CDS             301..585
                     /locus_tag="PW101_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515008.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="PW101_002640"
     CDS             588..1004
                     /locus_tag="PW101_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515009.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="PW101_002641"
     CDS             complement(1141..1860)
                     /locus_tag="PW101_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2515010.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2513..2608
                     /locus_tag="PW101_002642"
     CDS             2513..2608
                     /locus_tag="PW101_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL2515011.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2910..3155)
                     /locus_tag="PW101_002643"
     CDS             complement(2910..3155)
                     /locus_tag="PW101_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515012.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1038 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt atttcattgg tccaagctaa aaaactctta
     2881 gaagcgattt tagagccatt taaacccact taattatatt ctccatgaaa ttcgctccat
     2941 gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca ctagttgcat taatttttcc
     3001 aacgatcgtt ttctttcggg tgtcatattt ttatattcca acaatttttc gtctgttaaa
     3061 tcgaacttga ttgcttgaat tgcttgctct aatttttcta agttttgaaa atattcgtaa
     3121 ggaaattttt ccattttttc tttctccagt ctcacaattt ttctagaaat aaaatcactt
     3181 ctttgccgtc tatactctc
//
LOCUS       DANKHL010000243         3099 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000243 DANKHL010000000
VERSION     DANKHL010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.254"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            69..824
                     /gene="fabI"
                     /locus_tag="PW101_002644"
     CDS             69..824
                     /gene="fabI"
                     /locus_tag="PW101_002644"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDL2515013.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(934..1947)
                     /locus_tag="PW101_002645"
     CDS             complement(934..1947)
                     /locus_tag="PW101_002645"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDL2515014.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            2112..3032
                     /locus_tag="PW101_002646"
     CDS             2112..3032
                     /locus_tag="PW101_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDL2515015.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          900 a          624 c          590 g          985 t
ORIGIN      
        1 tattttagct attattctct atttttttac gactatcaaa tttttatcag aaacaaagga
       61 gagtcattat gtcatttcta gaaggaaaaa aaatcgtcat tatgggcgtc gctaacaaaa
      121 aaagcattgc ttggggttgt gctgaagcaa tgttgaaaca aggggcagaa attatctata
      181 cttaccaaaa tgaacgaatg aaaaaatcat tagtcaaatt ggtcggcgaa gaagcattca
      241 ctgtagaatg tgatgtttct agcgacgaaa gcatcgaaaa agcaatgaat gccatcggtg
      301 aatatgctgg ggagatccac ggccttgtcc atgcagttgc ttacgccaac aaagaagaat
      361 tgtccggaaa tgtgtctgat atttccagag acggctatca attagctcaa gatatcagca
      421 gctactcttt aattgctgta tcaaaatacg cacaaccgtt tcttgcacca aacagcggga
      481 tcgttagcat gacttatcta ggttcagaac gcgcaatccc gaattataac atgatgggta
      541 ttgcgaaagc ttctcttgaa gctgctatcc gttatttggc agctgagttc tcgcctaaag
      601 gaattcgggt caatggaatc tcagctgggg caatcaaaac attagctgtc acaggtgtta
      661 aagactatca aaaactgatc cagttatctg aatctcgtac gcctgatcag caaggtgtga
      721 cgatcgaaga agtcggtaat gcttgtgctt tcttaatcag cccactttcc agcggaatca
      781 tcggtgatat tatctacgtt gacaaaggtg tccatctttc ataaactttt gttgcattct
      841 ttaaagtaaa acatataaaa atcaggaaaa aactttttaa agtctcttcc tgattttttc
      901 tttttacgtt tttttcaact cattactttg aaattattcc acgacttcaa tccagccttc
      961 tggtgcttct atgtctccaa attgaatacc tgtcaattca tcatacaacc gttttgttac
     1021 tggtcctact tccgtttcac tataaaatac atgaagattg ttattgtgat aaatcccacc
     1081 gattggagaa ataacagccg ctgtaccaca tgcaccagct tctgcaaatt gatctagatc
     1141 atcaatgaaa atatctcctt caattgcttg cagtcctagt cgttcttcag ctaactgcaa
     1201 caaggaatat ttcgtgatac ttggtaagat agagggcgat tgcggagtga tgaattttcc
     1261 gtcttttgta attccaaaga aatttgctgc acctacttct tcgattttcg tatgtgtggc
     1321 tggatcaaga taaacacaat ctgagtaatc cagttctttt gcttctgttc ctggaagtaa
     1381 actcgctgcg taatttccgc cgacttttgc tgcaccagtc cccttaggag cagcacgatc
     1441 gtaatcagat acaataaaat tcgttggttt tagtccacct ttgaaatacg ctcctactgg
     1501 cgtacagaag acgacaaata aatattctgg agctggatgg acaccaatgt tttcaccgac
     1561 cccaatcagt aaagggcgca agtataatgt agcgcctgtt ccatagggcg gcacaaaatc
     1621 ttcatttgct tttaccactt gcttgacagc atcaacgaac atcttttcag ggaccattgg
     1681 catcagcaat cgtctagcac tttgattcat ccgtcgacta ttttgttcag gtcgaaagag
     1741 attgaccttg ccgtcttttc gacgataagc ttttagacct tcaaaacatt gctgaccata
     1801 atgaagcgct ggagagcctt catgaatcgt caggtaatta tcttctgtca attctccttc
     1861 ttcccattgt ccgtctttcc atttggcaat aaaacgccaa ggagttttga tataagaaaa
     1921 acctagattg ttccaatcga tttgcatata cttaccacct tcgttatttt ctccacattt
     1981 tatcattatc catattattt gtcattttat tttcagcttt tttccttcat tatagttatt
     2041 tttgctattt ccttttttga aaacttcttg gtacaataca gaaagaatca aattttagaa
     2101 aagaggattt tatgttttca ttattagcaa ctgccggtac gacagactct tctggcgaga
     2161 cattgacaga tcaagctgtc agcaaagtaa atgcgtttca gcggttttgg gacgggattg
     2221 attgggacgc cattttggct gcctttatcc aaaaaagttt atatctgatt tttttgatcg
     2281 tccttttttg gattcttcac cgtgtgggaa aatatctgat tgataagtct tttaatcagt
     2341 attctaaaaa agccattcta aacgagagcc gcttaaagac tttacatagt cttttaaata
     2401 ctatttttca ttataccctt ggtttctttt ttatctatgc aattttatct gctatcggtg
     2461 tgccgatcgg atcacttttg gctggtgccg gaattgcagg tgtggcaatc ggtcttggtg
     2521 cccaagggtt catgagtgat gttattaccg gattttttat tatcatggaa caacagatgg
     2581 atgttgggga ctacatcaag ctagcaaact taacgattga aggaaccgtc gtatctgttg
     2641 gtatccgtac ccttcagctg aaagcaacgg atggtactgt ccatttcatc ccgaatcgaa
     2701 acatcaccac gatcagcaat ctctctcgtg caaatatgca agtactttta gatattcgga
     2761 ttgttcctga agaaggatat gacaaaatct atgagatcat tgatcgagta aatcgtacat
     2821 tagctgaaaa ataccaagaa gagctccaaa ctgaaccttc tatctttgga ttagtggatc
     2881 ttggaaacag caattttgcg attcgtacgg tctgctacgc attgaacgga aaacaatatg
     2941 ccttaaaaga agagttcttg agctcctatg tcaaagaatt gacagcttct ggtttcacga
     3001 ttccgaacag tccaattgct gtcggaaaat aatttggaca aacgatgcaa cgaatctcac
     3061 taaaaagagc gtgggacaaa agtaaagaac acttttgtc
//
LOCUS       DANKHL010000244         3092 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000244 DANKHL010000000
VERSION     DANKHL010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.255"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(309..923)
                     /locus_tag="PW101_002647"
     CDS             complement(309..923)
                     /locus_tag="PW101_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2515016.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1529..1906)
                     /locus_tag="PW101_002648"
     CDS             complement(1529..1906)
                     /locus_tag="PW101_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515017.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2206..3087)
                     /gene="galU"
                     /locus_tag="PW101_002649"
     CDS             complement(2206..3087)
                     /gene="galU"
                     /locus_tag="PW101_002649"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL2515018.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          936 a          647 c          477 g         1032 t
ORIGIN      
        1 tcatcaacgt gtattaagag acaagaaaga atccatatgg attctttctt gtctcttttt
       61 ccactacttt ttatttttct gacactacat attcttataa ctcatttctc ataactgtga
      121 aacaacttat aaaaataaca aactttaaac aaaacaagct taaaatcaaa gatgatccat
      181 cttaaaataa ctaaaacaaa aaaagtagtc gttgttaatc ctcattaata atcgttttct
      241 ttttttcaaa cgctccattt ttatgtggaa cattattttt ttaaggaact ttgtcaacat
      301 gtggataact attttccact tgtgtcctgg actagctgtt ttgctgtaaa atacaaaact
      361 tgctgcagca aaaagatcgt tgcaagagag gctgttccgc agatcgctgg atctagattt
      421 ggcaaagtca agactgcatc atgtaaattg gcgtggttgt ccaaaaccaa atcagcaaat
      481 tcaaataatc gcaaaccact gctatgttgc gatttgatac ttcgtgacaa atcaaatcca
      541 gtaacaatga ttaatggatg accattttct ttgatccact gagctagttc gatgattgcc
      601 gggtttcttc cttcattcga aagaagaaga aaaatgtctt ctgatttgac agaaagctga
      661 tcaataattg cctgtcctgc accttctagc gtttcataga cacctccggc tggattttca
      721 atcaatatac ttggtacaag atccggggtt tgatcccaaa attcaaatgc tacaccactt
      781 agtgtgcggc tagcatatat ctgcaatcta ccaccttgat taatttgttt cgcgatccaa
      841 gtgccagctt gaagcatcag cggctcttct atccttaatt tctcttccaa ttggtgataa
      901 tagtcaaaat aacataaact catttatctc tcccgcctag ctatacttga catgaataga
      961 aacagctgcc taatctaagg aagtacataa aaaaacagta gttagacagt aacacatgtg
     1021 gtctttgctg tttcaaacac aaaaaaggca ttgaacgaat acgcccaata ccttgacata
     1081 gcaaataaaa caacgtgcag accatggaaa atatctacat aggggtagaa ttttcaccct
     1141 agattaggta caataaatta ggtcgatgca ctgcattgac tatggcaaaa tgatcggtca
     1201 gggtgtatgt gtgtgactgg tcattccctg attcttagcg ccaactaagg gtcagggagc
     1261 ctttttttat tcagctgttt ctatctgaat ttattgtaag taatctctta tccaagtgca
     1321 agcaagaaaa tacaaaaaaa cattggtatg atttatatat atataaataa cataaaaaaa
     1381 ggaatggctt atcccgatac aacaagcatt cctttaaaaa agattctttt atataagtga
     1441 aaaaagcttt tttccaccaa ttaaataaat ctctcatctc aatagtttat ccattatttt
     1501 atcttttttt gcgtatacct actaaatatt accgtcttct tctcgttctt ctactttgtt
     1561 cgagagaatc tgtactagaa aaagtagttg tatgtctacc ttttctgcct atccgagact
     1621 ttcttttcat atatgggata taaatgatta gatacagtac agcaaaaaat aaagcaaata
     1681 cgcaagcttc ccaactacct aacgccaatt caaccatcaa ggtagcaacc ccaacggatg
     1741 ctgcatacca gataaaaatc agaaacgttt ctttttttct gaccatgcga aacatgataa
     1801 atagactaat aagcaatagt ggcaacacaa atatccaatt gcttccttcc atcagccact
     1861 tgagtatatc aagcagcgac tgtgcaacag cgactaattt tcccatagtg aacacacacc
     1921 tttttttata attaaaaaat tcgccttttt attctactat gaatcttctg gcttggcttt
     1981 catttctctt atgtaatcat tttcttttat acaacttaaa aaaaaattaa accattagca
     2041 taatattttc tgaatctatt cacgaaagtc tttttctatc tttcgttttc cacgactcaa
     2101 ctgatctaga gtcaaaaaac taaaacgaca gttgcgcaaa tgacgcacaa gctgccgctt
     2161 taataacaaa tagcctttca ttctcagcag gccacttatt tccagttaac tttctgttct
     2221 tagcttttta ctcaactgga taatatatgc acttaatgaa gtcccaattt ccgaatgttg
     2281 aagtccatat tcaatatttg tcttcatata tcctaactta tcgcctacgt cgtatctttt
     2341 tcctttaaac tctctcgcat agaccgcctc tgtctggtta agagtctgaa gtgcatccgt
     2401 tagctgtatc tcgtttcctg ctcctggttc ttgagtttct aatatctcaa atattttagg
     2461 agtcagtaga taacgtccga taatggctaa attacttggt gcttttccca tttcaggttt
     2521 ttctacaaac ccttttactc tgcaacgacc cttttcgcta tctgttttag gatcaataat
     2581 tccgtattta gatgtttcat tttctggtac acgcatgacg gcaacagcgg aagcttgtgt
     2641 ttcttcataa tccatcatca attgttgagt caatgggata tcatctttca tcaaatcatc
     2701 ccccagcatg acgacaaacg gttcatttcc tataaatgct tttgcttgaa gcacggcatc
     2761 ccccagtcct cttggatgag attgacggat gaagtgaaga ttgacttgag tcgtttcttc
     2821 aactaaagcg agtaattctg ttttttcctt ttcccgcaaa ttgttttcca attcgatatt
     2881 tgcatcaaag tgatcttcaa tgggtctttt ttcttttccc gttacgatca agatgtcttc
     2941 gattcccgaa tccaatgctt cttccacaat aaactggatc gttggtttat ccacgattgg
     3001 aagcatttct tttgccatgg cttttgttgc cggcaaaaaa cgtgttccta gcccagcggc
     3061 aggaatgact gcttttttga ccttcacttg ct
//
LOCUS       DANKHL010000245         3074 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000245 DANKHL010000000
VERSION     DANKHL010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.256"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            80..346
                     /locus_tag="PW101_002650"
     CDS             80..346
                     /locus_tag="PW101_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDL2515019.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            373..672
                     /locus_tag="PW101_002651"
     CDS             373..672
                     /locus_tag="PW101_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HDL2515020.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            846..1397
                     /locus_tag="PW101_002652"
     CDS             846..1397
                     /locus_tag="PW101_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HDL2515021.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1543..1836
                     /locus_tag="PW101_002653"
     CDS             1543..1836
                     /locus_tag="PW101_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515022.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1829..2125
                     /locus_tag="PW101_002654"
     CDS             1829..2125
                     /locus_tag="PW101_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515023.1"
                     /translation="MAKLIVLGGLIYGGYWLYQYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            2200..>3074
                     /locus_tag="PW101_002655"
     CDS             2200..>3074
                     /locus_tag="PW101_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDL2515024.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYS"
BASE COUNT         1146 a          429 c          624 g          875 t
ORIGIN      
        1 agattgggct atttttatgg caaaggaggg aaaagaaatg tttcaaaata aaggataacg
       61 ttatgtcaca aagggagtaa tggacagttt gccggtggaa ttacaagctt tatgttggaa
      121 tctaatcgat caaaacgttc aaaagcaatt tcctttagac tatcttcaaa tttttgaatt
      181 ttctacggaa aaaggaaatc aaaagttggt tcatcgtcaa gaagaaccgg aagaaagaaa
      241 agagtatctg atttcaccta agcttcgttt gaaatcagtg tctcaaaaaa tatgggccat
      301 tgattcgggt gagtatcaaa cgatgttatt accagaagaa tactagtatc taaaagaaag
      361 gaacgaaagg acttgttaga aacggctatc gaagtactgg aaaagtgtgc acagctggtc
      421 actgcttctg aagagtgggg ctatgaatcc gttacgatgg aaaaagagga aattgaaatg
      481 ggtatgcttc ctgaagatgt tcatctacca cgtttagtga tgacacatct ttatatttat
      541 tgcgcaccag aagatggaaa agactatgta gtttatttca ttacagatat taccagccag
      601 cgagaatttg ttcgtgggtt actggtagaa ggacgattgg tttggagtca gattggagga
      661 acggatgaat aagatcgatt atttgagaat gattgcgaaa tgtttagaag atatccttac
      721 cgcgattcat caaattctga agaaaaaagg agaaagaata tgaaatattt tcaactagta
      781 ttgacactta taacggcaat ggtaattgaa gtgcttgaac aaaaaatagc ataggagaaa
      841 aagagatgat tgaaaaagaa ttagtgacca cacaaacaga agtttggcga gatcctcatc
      901 atacccatcg ctatgttttc aaacgacaat gggcgatcaa aggaaaagaa gcggaagata
      961 aattagcagt agtcattact attcgtccaa cagatacaga accatttacg aatgatttga
     1021 gtatgttatt aatcgaaaag aatatccgac aattaggatt tactggtttt attgctgtga
     1081 atcttttttc gtacacaaaa gcgaaaagtc ctcgggtatt tcttcgagga aatgatgaac
     1141 aatctttgga aatattaacg actgttttta cagaaaagaa aatcaatacc attatttttg
     1201 cttgtggttc gattacagcg acgagtcaag tggcttatga acaagcaaag actatttacg
     1261 atcaattgag tgcgaaacaa aagaagatga ccaaagtatt gataaatgca gaaggtaagt
     1321 tatcccaccc actaagtatt catgttcgta atgaatggca attggcagat agtcaattat
     1381 tatttcaaga aaaataacat tttattttct catgttactt ataagtagtt ttacatgctt
     1441 tttgacttta aaaatacatg tgatgataca tgttatatga attatcaatg tacccagtaa
     1501 tatgctattt tcattatgta aaaaataaca gggggtgtgt agatggagat tgatgaaact
     1561 tgtttgagta actcttataa aagtatgaat gacttttttg aggaaatgga actggattca
     1621 gaattgctct atcaatttta tcttgctttt cgagggcaac agatttcttt tccaatgaaa
     1681 atgtacgatc gtgagttagt tagaaaaaga atcgaaaaca tgatagaaca ggaaaaaact
     1741 gtagatatcc gtcaattgac agaagtttat ggtttctctg caagatggat tcaaaaagta
     1801 attaaacaaa aagaaaggag acgagtacat ggctaagctg attgtattag gtggtttaat
     1861 ttacggtgga tattggttat atcaatatat gcttgcgcat ccgaatatag ataaaggaaa
     1921 agtaattaag acagttgggg taatcatagt tggactcata gctctaacta agaaaaataa
     1981 tactaatagc ccaaaaataa tgagaactta tcgctgtaga ggatgtggaa gggaaatttc
     2041 tactagtttt aatccatctg agagtcctca aggaagagac cgctgccata cgggctatcg
     2101 acattattgg gaaagaatcg attaaaagat taccatttag atcaattaat tgtctgatag
     2161 aataagtacg tgagggagaa attgaaaggg ggaaatataa tgaaaaaatg gctgttatta
     2221 ctaataggaa tgggaatttt attcactggt tgtacatctg agaatacgag gactaaagcg
     2281 actatagaaa ctgatacaac aattgagact aaaaaaagta aaacgcaatc aactgaacaa
     2341 actacaacag aaatgaagca atctgtggaa acaagtgcaa gttcaatcac tgaatcttct
     2401 tctgaagagg ttaagaatac tttgtgggat acgaataaag cttccaaatt agaaactttt
     2461 gtcacacaat ggggaaagac attaggtcaa gaatataaaa gctacaatct tcagaataat
     2521 gtaagtctgt atggcactcc tttaccacaa gccgtgatta atggcgattg gaaaatggca
     2581 atcaatgaag caccagttac cgtacaatgg tcagaagatg gaactggtca tgcagatttc
     2641 aatttagtag cagtttattc tgatgtagaa actggagaat atttagggca gcatgtttac
     2701 tttttcggtt ttcaaaatgg acaacctaaa gtttatttaa cgcaacaaaa tcaaggtaat
     2761 gaaaataatt atctgtactt taacgaaact caaaatcaac agttaaaaca aggtttcagt
     2821 gatattgtga atggacaaac tcctcaaaca ccagtagtag aagaatctac tcaacaagca
     2881 acaactacaa cgacacaatc tacaagtagt tcaggaattt caagttggga agctgctaaa
     2941 gaatctgtgc taaataataa agctttatgg ttgtatgatt ctcctgataa aaatgtatca
     3001 aatactaatt taattagcac aaatgaatat atgaaagatc taatgcaaga tgaaaatgga
     3061 agctactatt caat
//
LOCUS       DANKHL010000246         3051 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000246 DANKHL010000000
VERSION     DANKHL010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.257"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(534..1100)
                     /locus_tag="PW101_002656"
     CDS             complement(534..1100)
                     /locus_tag="PW101_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2515025.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1100..2056)
                     /locus_tag="PW101_002657"
     CDS             complement(1100..2056)
                     /locus_tag="PW101_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDL2515026.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2056..2712)
                     /locus_tag="PW101_002658"
     CDS             complement(2056..2712)
                     /locus_tag="PW101_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL2515027.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT          970 a          590 c          539 g          952 t
ORIGIN      
        1 attgaaacat ccatctggaa tttatctttt ttactgcttt gatcataaaa gtctgcatat
       61 tcatcactga tattttttct aataaattct tccatcataa aaatcacctc aaaacgatta
      121 tacgaactta cgttcttgtt gtaaagcgat atttgagggg caaaaaaggg gcataatttg
      181 actgatatta agttagctat tgatatatga atgacataca aaaatataaa atcttttaaa
      241 aatgcttata tatcaatgtt tttgccattc ttttatatat ctgttatatg tcaaaaaaat
      301 gtgttcaatt tcaatttgta tttgaataaa gaggttgtga aaaagctgtc ctgcatcaag
      361 taataagaac agccaatcaa aaacagcttc tcatgttttt gatttggttg gacttattac
      421 cgcagatata agcttttgag caccgtgaat aaagaggtta tgaaatcaac gctctaacct
      481 gagtattaga gaaaaaaacg caaaacatat catttatgtt ttgcgttttt tatttatctt
      541 tttttctcaa tacaatataa aataggcggc tggttctttt ggttgataaa ccgataattc
      601 agcacattat atgtttcctg tggcagtttg gaacaaaaag aatcaaccat atccagttct
      661 ttttctccac cttcatgacc atagtagacg acaaggatca atcgtccgcg aggcacaagt
      721 cgtttcaata tttcttccat agctgtgcgt gtagtctctg gcatagtgat gattgcttta
      781 tcactttttg ggagataccc tagattgaaa atccctgctt tgagattttg ttgttcagct
      841 atcacttctc ctaacgtttc gtggccttgt agaaacaatg agacacgttc ttctaactga
      901 cgctgttcta gcctttcacg agtagcatcg attgcttgtt gctggatatc aaaactatag
      961 acatgaccag tttttccgat gtgttctgct agaaaaaccg tatcgtggcc attgcccatc
     1021 gttgcatcta cgacgtgatc ccctggttgc aagatttctt gcagtaatgt atggctgaaa
     1081 tgcaaagcag tttgcaacat cagacgctca cctttttccg tacatcatat tttccttgga
     1141 agctatctct gcgaagaagt tcttcatcaa ttgcattcaa gacttcccat tttttcaagc
     1201 tccacattgg tccaatcaag gaatcccacg gcgcatcccc tgtcaaacga tggatgatga
     1261 tctcttttgg tatcatttcc agctggtcgc aaatgacatt gacatagtca ggacggctca
     1321 ttaattgaag gcgaccttca tgatagtccc gcaacattct cgttttgcgc atcagatgca
     1381 tcaaatggag cttgattcct tggatatctg aatctaaaat ggtgcgtctg acattttctt
     1441 tcatcatttc taaactttcg cctggtaatc cgttgatcaa atgcgtacaa acacggatat
     1501 tatgtttgcg taatttagcc acgccatcta aataagtttg ataatcatgt gctcggttga
     1561 tcagttttga agtttcttca aaagttgttt gcaatccaag ttctacccat aaatacatcc
     1621 gctcgttcag ttcagcaagg taatcaacta cttcatctgg cagacaatcc ggtcgtgtac
     1681 cgactgataa tcctacgaca cctggtaaat tgactacttg ctcaaatcgt tctttgatta
     1741 tttcaactgg cgcatgggtg tttgtaaaat tttggaaata aacgatgtat tgttggacat
     1801 gaggccattt ttgatgcatc atgtcgattt ctttctggaa ttggattgga agagggtctt
     1861 caggtgcaac gatcatgtct cccgaaccag atacactgca aaaagtacaa ccgccatggg
     1921 caacagtccc atctcgattc ggacagtcaa atcctccatc gatcggcacc ttgaaaattt
     1981 tcccgccaaa ctcatttcgt aaagcgtaat tccaagaatg atatctttta tttccttctg
     2041 aataaggaaa agtcattaaa agtccttctt tctgtaagag ttatctattt gttttctttt
     2101 ttctctatac actggataag tcaggcacag ccaagaaaga ctgagtaagt agccgccgat
     2161 gatatcagta ggaaaatgaa cgcccagata aatgcggctt atcccgacac ttactataat
     2221 aaacatcatc agcaattgaa gcaatgtgcg taaggttctt ttttgaacaa aagctggaag
     2281 cagaaatagt aaagaaccgt aaagaatcat actgcctgct gcatgtccac tagggaaact
     2341 gtaacttgtt tcaactacta agtgcgccaa tgaaggacgc tctcgcatga aaaatagttt
     2401 gatcaccggg ttgatgattc cagcaattac aataaatcca gaaacgaacc aaaatgtttc
     2461 tgcccttttt ttcttatacc acatccataa agccgcacaa gcaaataaaa tgatgacggt
     2521 aagaggatta ccgaatttag tcataaagag ttggtagctg ttcagatcag gataagaagc
     2581 tctgacaatg cctgtaaacc actgatcgaa accagaaagc cacgacagat ggaactttac
     2641 gatccagccc aaaatgaaaa acaggacaag aaagaagcta ccagccacct gaaatgatgt
     2701 tttatatttc atacaattcc tcctttgaag caatgactta ttatagcatt tttggtccag
     2761 cgaaaaaaac ggtaaagaga taaaaccaat gtatgattcc tctttgaata accaatattc
     2821 ttataaaaaa aataactgta atcggaaaga cattttttgg taatgcaaat gaaatgttag
     2881 ggaaataaaa tcagtgtttt tcgaaatcct tttaaacaag aacgtagacg taggaagaac
     2941 ttgaaaatga acagaagttg ttcattacgg taaattacaa tatgattaca aaaaacaaca
     3001 aagctcttaa ataactttta ataaatatta agagaagtta taattattaa t
//
LOCUS       DANKHL010000247         3051 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000247 DANKHL010000000
VERSION     DANKHL010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.258"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(266..1576)
                     /locus_tag="PW101_002659"
     CDS             complement(266..1576)
                     /locus_tag="PW101_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL2515028.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1588..3006)
                     /locus_tag="PW101_002660"
     CDS             complement(1588..3006)
                     /locus_tag="PW101_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2515029.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT          954 a          629 c          475 g          993 t
ORIGIN      
        1 cgaaatcttt cagcaactca tttatcataa cttatcgttt gttatttgtc aagaactttt
       61 ttgaagtttt tttcgataga atcaagcttc gcaagtggtt ctttttaacg acttagttat
      121 cttatcacgt ctaaaaacat gtgtcaactc ttttttggaa ttttcataaa atgtttcaat
      181 gaagcgcgac aacgttgttt aatatatcat taaaaaaaat aaaggtcaag ctaaaatatt
      241 atttaagctt gaccttatta taatttcaac gattgtcaaa agtattgttt gcagctgtgt
      301 tgccagtttc tatagataaa tcacagcttt tgaaacataa gttttgtact gcttcgattc
      361 gcccgaattc ttttgcagtt atttggcaat gagaaaagca gacatcttct ataggctttt
      421 caggaaatgc tactaaatca aaagctcggg aaggaagtga gtaacctgga gaaaggcatg
      481 cctgtacgtt tttgatgtgt atgtctttga ctttcgtcat ttgctttgat tcaggtattt
      541 cttcagccac tgcttgccaa ctttctggca tttgagcaag gtcattgcta ttttttctat
      601 tgtattcatt atgccagtcc attatccaag aaaaaggaaa ttgtacatca atcatattca
      661 gattttctac cctgatattt tcgatcactc cccctctttc ttttgaagat ttcattcgaa
      721 taccacagtc tgactgaaaa aaatcgatat cgtgaatata tacgtcacta actccagcac
      781 taacttcgct accaatagtt acaccgtatc ctgaacggat tttacatcta gcaacctcga
      841 ttcttgatga ttttcgattg actcttgcac cattgccatc tctgccagac ttaatagcaa
      901 tacaatcatc accacaggcc aaatcacatt catagacacg cacatttgtt gaagaatcaa
      961 tatcaatccc atctgtactt ggtccatcat tgttgcggat gattactttt tctactaata
     1021 catcgttaga ataagtgatt tgcaagttcc aaaaacctga tttctgaaaa atcaaatcac
     1081 gaacaacaac atgttctgca tgatacagta aacaagcgcg tggacgttta atagcataat
     1141 ccgcaatcca acgaagtccc ttttgatcgt attcagctct tgtcccgctt ttttgatctt
     1201 ttccccaata cagctcccac caatggtctc cttgaccgtc gattattcct tttccttcta
     1261 taagaatatc tttcgccgag agaacattca aaatcgctgc tggccatttc atttctacgc
     1321 cggctactcg tgtatcgatt tctggaaatg cttcgatatc tgttctcccc ttgaggactg
     1381 caccttcttc aaagataaga tgactcttat cttttaaaaa aagggctccg actaaataaa
     1441 tgccttttgg aataaataca ggtaagtgtt ctttcgcacc ttgatcgatt gctttttgca
     1501 gtattattgt atcatcttgt tgagtgtcgt ttgttttaaa gttcaaagca ttgataatcc
     1561 ctttttttgt ccgcaatacg tttctcccta tctttcattg atttttttca attcttcacc
     1621 aataaataca gtttttgaac gattcagtgg gtaacctact agcaaaatga ttgcggatac
     1681 aaataagcag aatgctggta acaagtttgc catcgtataa atcctttctt gtactaaaac
     1741 tgattggatg gctccacctg ttgtagacga ttggtaacca atgagtgcca gcataaatcc
     1801 gcctaccgct cctgcacatg cttgtcctac ttttctcgca aaagaattta ctccatatac
     1861 tgttccatct tctcttaagc ctgtcttata ttgatgataa tcaatcacgt cagtaatgaa
     1921 tgcccagacc atcaaattga ataaaccaga tcctaaagta gcgataaata aaagaatcag
     1981 atatacccat gcatttgtta tatgaaggaa atacatcagc aagtacatac atgaagaagc
     2041 aagtaacgca aatacgcttg cttccttttt accaaataat tttgtcagct ttgtagcaaa
     2101 tggtgctaaa actactactg ttccgtaagt aaataaaaga gcaacagaca ttgcttgttt
     2161 attatggaag taatcattga ataaataagt catattcgtc ccagctaaaa tctgattgat
     2221 cacaatgaaa atgtcaacaa tgactaaaat aatcagtgct cgattagcta acaaaccttt
     2281 taccaactga gaagcagata ctttttcgtt tttgacaact cttactcttt cagtagtcaa
     2341 tttacaagtt aacatatatg cgacaaatgc tattgcagca catccgattg aaatccaaaa
     2401 gaaatgattc cctgaaacga cttgttgtcc actcttgtta tgggtataca ttaaaatcgg
     2461 aatgaagaat cctgtagttg atccgccaat cgcagaacca atgcttcgga acgtggacaa
     2521 cgaggtgcgg tcattaggat ttgagctaat tgctgctgcc attgctccat acgggatatt
     2581 gattgttgag agaaaaatcc cataaacaat gtatgaaaca aaaatataag caatcttcac
     2641 cgtcattgga agatgttgga cgaatggcaa aaacaaaatg acagtcacta agcaaaatgg
     2701 atacttcatt tttctgatcc aaggaataaa tcggccagtt tctgttaact ctgagacatc
     2761 acataatctt ccaaccatca catcagcaaa cgcatccaaa attctcgaaa tcaagaataa
     2821 cgtacctacg attgcccctg aaacgccaag aacattcgta taataaatca tcaagaaact
     2881 attgactaaa cctaaaataa aacagttacc tagatcaccg aacatgtagc ctatcttatc
     2941 ggaaatccca aattcctttt tttctttttt tattgtattt gctgtaacag cattttgtga
     3001 aagcatttga attcctccta cctcttctag taaacgtttt caaaattata a
//
LOCUS       DANKHL010000248         3037 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000248 DANKHL010000000
VERSION     DANKHL010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.259"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            21..329
                     /locus_tag="PW101_002661"
     CDS             21..329
                     /locus_tag="PW101_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515030.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV
                     "
     gene            331..753
                     /locus_tag="PW101_002662"
     CDS             331..753
                     /locus_tag="PW101_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515031.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            773..2362
                     /locus_tag="PW101_002663"
     CDS             773..2362
                     /locus_tag="PW101_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515032.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            2374..2709
                     /locus_tag="PW101_002664"
     CDS             2374..2709
                     /locus_tag="PW101_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515033.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            2850..>3037
                     /locus_tag="PW101_002665"
     CDS             2850..>3037
                     /locus_tag="PW101_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515034.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         1212 a          439 c          612 g          774 t
ORIGIN      
        1 tttaaattgg aggaattaat atggaagtaa gaataagaaa tgtagatgag aaaatcgtaa
       61 gagaaattga tcgaagatgt gacgaactaa gtaaccgaac gggtaataaa tggagtcgta
      121 acgattattt aaagctactc gttgaaaatg attttgatcg tcctttgatg gaatataaac
      181 aagataaatt tgatcaatta ttaaatcagt taatagataa attttctttt gttcaaagtc
      241 ataatacaaa agtattagaa gaatatattt gtcagaataa tcaaattatc gaacttttaa
      301 ttgaacaaag tagaggagga gaagtttaac atggccaaag ttatagacaa gcatatttat
      361 ttttatgaaa cacatataga tgttctagat gaaattattg caactcatgc agaggttaaa
      421 aactatagtg aagccgttcg gtttctatgt atgaattatg attcagaagt ggagaaaaaa
      481 gtaaattcca atcaagcaaa gttgaatgca atgggaaaag aaatttctat tttaaccgaa
      541 atggtttcag agtttgcgga tctccatttg aaagatacag ggattttaat tggtacaaat
      601 tcaactgttt accaagaagc aaaagagcgt gtggaaggca agataaaagc aaatcaaacg
      661 aagaaatatt ctcctaagat aaagaaaaca ataagcgaat cagtgactac aaaaagtgaa
      721 gtatttgatc caacaaggtt cttaaaacga taaaaattag aaggagaatg tgatgagtaa
      781 atcacctgca gtcattctca cttgtcaatt taccttgccc aataaaaaat ctttttcaac
      841 atacattgat tatatgacaa gagaaaaagc gctggaagaa aaggaacgac gtacgcccga
      901 ggaagaacag gaactgcgtc taatcaagaa tgctttagag gatttttata ttcctgaagg
      961 agaaactttt tcggagagta agacgggtaa agaattcagc gaaacgtcga atgaagcgca
     1021 atcgttgatg aaacatggaa tcaattttga caagttagaa gaacaagatt ttaccaaata
     1081 tctttcttat atgacacgtc actatgcgtt agaacaaaag aaagtactga cgcatagtga
     1141 acaaaaagaa taccaacgct tgaaccaagc aataaaaaaa tatcaaacag agccacacaa
     1201 aaaagcttca ggaaatcttc ctggagtttt ttctatggct tcagacgaag tgaaaggaag
     1261 tgatctgaag gagatcaagc aaaccttcca aagaggacaa caaaaaggct caatcattta
     1321 tcaagatgtt gtctctcatg atcattcgta tcttgaaaaa ataggattgt acgatcccaa
     1381 aacagatact ttagatgaag aaggtttaaa agcagctgga agaaaaatga tggagacatt
     1441 gttcgagaaa gagcaactga atgaaacagg ctactggatg gcaacgattc accgaaatac
     1501 caaacatatc catattcatt ttgcagttgt tgaaaaaaaa aatacgcgta aaccatacac
     1561 tgagattgaa aatggtgtga gttatatcgt accgaaaggg aagagaaggc aagcaacatt
     1621 agacgaaatg aagactaatt atgtacatga gttagaacgt ttttcttatg aaaaaaatcg
     1681 aatgggtatg gagaaacgaa atttattgac acggaaaagt gatttgcgta acaccttaac
     1741 caaagtagtg aaagagccag tacgttatga tcgacacgca atgagcttat taactgaagt
     1801 atataaaaac ttacctaaaa agcgttcgga atggcattac ggggatgaaa atcgaaccaa
     1861 actttcacca tacacaagag agaaactcga tgagttaacc caatatgtgt taacaaaaga
     1921 acctggatat caagaatata tacagatttc aaaagcattg aaagaagaag caaaaagtct
     1981 ttatggggaa agcaagagag aatcaaagga tgcagaaaaa aatgctctct ttgatttaaa
     2041 aaagcgcaca ggaaatgcca ttctaacaga actgaagaaa caggatagtc aaataaaacc
     2101 tttaatagaa aggatggatc agatgcaatc tatttacaat gaacgatcct ttcgaacact
     2161 taaacagttg ccaacgaatg aatacaaata tagttacgaa gaattcatga agcgcaggaa
     2221 acaattccaa caaagagttg tcacgcaacg gagtttgcgt caattaaatc gaacgattaa
     2281 tgcccatgaa gaaaaatacc acgcaagtaa agcatacgaa gagcaacaac gaagaattca
     2341 aatggaacaa gaaagacagt aggaggaata gcaatgaaac aatatttaga tcaatggaaa
     2401 gttatagaag gatcattaag agaagaacga atcgaacaac ttcccgattg tttagaaaaa
     2461 gagcatttgt ttcagataag agaaatgttg ataaacgaac agtttgatcc aaatcaattt
     2521 ttagtcgttg agtacccagc tactggtgtg tactgttgta atcatgtgaa gggtgaaaaa
     2581 tattttatca ttcaagaata tgagggcaag ttagcgcctt attatactac ttgggaaatg
     2641 aatgaagagg ggatcaataa cttcccgtgt aaaagcattg aagagtctat ttcactcaca
     2701 gaatgttagt tgttaagtga ataaaagaag taaaagagtc ctgaacgtga ttgtccagga
     2761 ctcttttttt tcactgaatt gttttccgcc aattcattga gtggtgcgat ttccgtctac
     2821 attatatcaa aagaaaggag gaagaagaaa tggcttattc aacgattgga gttgttgttg
     2881 aaaaaagcag agacaatctt gtttttgtga cagaaattca aacaggcaga gcttttgtag
     2941 tcacagacaa agcggcaaaa gcatatcaaa gtggcgatat tttaacgtta aatctgacta
     3001 cgaagacgtt cgtcgatgcg gcagaagatt atccatt
//
LOCUS       DANKHL010000249         3027 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000249 DANKHL010000000
VERSION     DANKHL010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.260"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(106..1053)
                     /locus_tag="PW101_002666"
     CDS             complement(106..1053)
                     /locus_tag="PW101_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2515035.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="PW101_002667"
     CDS             1299..1763
                     /locus_tag="PW101_002667"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDL2515036.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="PW101_002668"
     CDS             complement(1825..2868)
                     /locus_tag="PW101_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDL2515037.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         1002 a          599 c          580 g          846 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa taaacggtgt
     3001 tccagaagta gcttctacgg aaataag
//
LOCUS       DANKHL010000250         2969 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000250 DANKHL010000000
VERSION     DANKHL010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.261"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..433
                     /locus_tag="PW101_002669"
     CDS             <1..433
                     /locus_tag="PW101_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDL2515038.1"
                     /translation="QEYILGEEYTIDVCLNFDSTPVSIVPRKRIATRGGEILKGQIIK
                     DRELIETTKKMLEKLNAIGHITIQCIKNEKGIFYLEINARYGGGAPISIKSGANSPEY
                     IIKMIDSKKNLRYRENYLDKMIALRYDQAIYLDEKGNVYND"
     gene            405..1121
                     /locus_tag="PW101_002670"
     CDS             405..1121
                     /locus_tag="PW101_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDL2515039.1"
                     /translation="MKKEMYIMIKAVIFDLDDTLVSEYDYVKSGYKCVARRLAVQKIF
                     PATFDEIYRVLLDLFAEDSKNVFNRLYDYFEIPYSKKEIKELVNVYREHKPDINFLEN
                     ADTLLTELRSRGYKLGIITDGYAITQRNKLDALKVDYLVDYICITDELGRELWKPHPI
                     SYLLLLEKFNLKAQECIYIGDNELKDFITAKSIGMQTIKIERVNGIYRESSLKFKRFY
                     QADKKIRSLWEIKNFLGVIS"
     gene            1118..2236
                     /locus_tag="PW101_002671"
     CDS             1118..2236
                     /locus_tag="PW101_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDL2515040.1"
                     /translation="MKSKRILLASPHMSEEGYEKEYVKEAFDTNWVAPLGANVNGFEK
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITIKGINANHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGD
                     DVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            2226..2846
                     /locus_tag="PW101_002672"
     CDS             2226..2846
                     /locus_tag="PW101_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL2515041.1"
                     /translation="MMRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLG
                     SPIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELP
                     ELWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKF
                     DLDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNIPEKFKGS"
BASE COUNT         1140 a          365 c          572 g          892 t
ORIGIN      
        1 tcaagagtat attttaggtg aagaatatac tatagatgtt tgcttaaact ttgattcgac
       61 accagtaagt attgttccta gaaaaagaat agcaactaga ggtggggaaa tactgaaggg
      121 tcagattatt aaagatagag aactaattga gactacgaaa aagatgttgg aaaaattaaa
      181 tgcaatcgga catattacta ttcaatgtat aaaaaacgaa aagggaatat tttatttaga
      241 aataaatgca agatacggtg gaggagcccc cattagcatt aaatcaggag ctaattcccc
      301 tgaatatatt ataaagatga tagattcaaa aaaaaatttg agatacagag aaaattattt
      361 agataaaatg atagcactga gatatgatca agcgatttat ttagatgaaa aaggaaatgt
      421 atataatgat taaagcggtg atctttgatt tagatgacac tcttgtgtct gaatatgatt
      481 atgtaaaaag tggatacaaa tgtgtagcta gaagattagc agtgcaaaaa attttcccgg
      541 ctacttttga cgaaatatat agagtccttt tagatctttt tgctgaagat agtaaaaatg
      601 tatttaatcg tctatatgac tattttgaaa ttccttatag taaaaaagaa attaaagaac
      661 tggtaaatgt atatcgagaa cacaaaccag acataaattt tttagaaaat gcagatactt
      721 tattaacgga attaagaagt agaggataca agctggggat tattacagat gggtatgcta
      781 taacccaacg aaacaaattg gatgctttga aagtagacta tttggtagat tatatttgta
      841 ttactgacga attaggccgt gaattgtgga aaccacaccc tatctcatat cttttattgt
      901 tagaaaagtt taatttaaag gcacaagagt gtatatatat tggcgataat gagttgaaag
      961 attttataac tgccaaatca ataggaatgc aaacaattaa aatagaaaga gtaaatggta
     1021 tttacagaga aagcagtttg aagttcaaaa gattttacca agctgataaa aaaatacgtt
     1081 cgttatggga gataaaaaat tttttgggag tgatttcatg aaaagtaaaa gaattctatt
     1141 agcttctcca catatgagtg aagaaggata tgaaaaagag tatgtaaaag aagcatttga
     1201 tacaaattgg gttgctcctt taggtgcgaa tgtgaatgga tttgagaaag aattagcaga
     1261 atacgtggga gctagtcatg ctgccgcatt atcttctgga acagcagcta tacatttagc
     1321 tttgaaagcg gctggggtcg gtaaagatga catagtgatt tgtcagtcct tgacgttctc
     1381 agctactgct aatccaattc tctatcaagg agctatacct gtatttgttg atagtgatta
     1441 cgaaacttgg aatatgaaac ctgaacattt gcgaacagca ctagaaaaat atcctaacac
     1501 aaaggctgta ttagtagtac atttgtacgg tttatcagct aatttagacg aaattactaa
     1561 aatatgcaaa gaatacgatg ttactctaat tgaagatgct gcagaatcat taggaacaac
     1621 atataaagga aaatatactg gtacatttgg cgactatgga atttattctt ttaatggaaa
     1681 taagataatc acaacttcag gtggcggaat gcttgtatcc aatgacaaag aaaaggtgga
     1741 aaaagtgaga ttttgggcta cgcaatctcg tgagaaggca cgccattatc aacacaatga
     1801 aattggctat aattatcgaa tgagcaatat cgttgctggt atcggtcgag gacaattgaa
     1861 agtattaaat gaaagaattg agaaaaaacg atatattcat agtttttata aaaaagaatt
     1921 gtctaaattg tcaaatatac aattcatgcc agaaaataat tggaatgaac cgaattattg
     1981 gttaagtgtc ataacaataa agggaataaa tgcgaatcat cttattgatg agttagacaa
     2041 aaataatata gaagcccgcc cagtatggaa acctatgcat ttacaacctg tttttaaaaa
     2101 ttatgattat attggcgatg atgtagcaga aactttattt gacttaggag tatgtttacc
     2161 atcagataca aagatgacag atgaagattt aaaatatgta gtatctacga ttaaaagagt
     2221 gatcaatgat gcgtaaaaaa actttctatc agctttttgg gaaaagaatt ttagatatcc
     2281 tattgtcagg aattgctctc atcgttttat cgccgataat cttaattgta ggttttttag
     2341 taagaattaa acttggttct ccaattatct tcaaacaaga acgaccagga aaaagtgaaa
     2401 aaattttttc catgtataaa tttcgtacaa tgactgacga aagagaccac aatggagaat
     2461 atttacctga tgaaattcga ctaactaaat ttgggaaaat gcttagagca acaagtctgg
     2521 atgagctacc cgaactttgg aatattttga aaggtgatat gtcgatcgta ggacctcgtc
     2581 cattattagt ggagtatttg ccactatata gtgagaagca acggaaaaga cacaacgtaa
     2641 gaccaggatt aactggactg gctcaggtca atggaagaaa tgctattagt tgggaagaaa
     2701 agtttgattt agatgtttat tatgttgata aaattagttt ctttaatgat cttattatta
     2761 taattcagac atgtaaaaaa gtaatcaaga aagagaatat taatacaatc aataacaata
     2821 tcccagaaaa atttaaaggg agttaaatac agcagcttaa tgcaagtttt gtgacttgca
     2881 ttatatttag gtataaatca aagcgagtat attagaatta tataacatat gttaaaaaaa
     2941 cgtatagcta ataaaagtat gattttaaa
//
LOCUS       DANKHL010000251         2963 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000251 DANKHL010000000
VERSION     DANKHL010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.262"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            34..1368
                     /locus_tag="PW101_002673"
     CDS             34..1368
                     /locus_tag="PW101_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515042.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1355..>2963
                     /locus_tag="PW101_002674"
     CDS             1355..>2963
                     /locus_tag="PW101_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515043.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLF"
BASE COUNT         1006 a          440 c          506 g         1011 t
ORIGIN      
        1 agtttttatt atggagaaaa gttttttaaa aagatagatg ctgctttttt atttagttgt
       61 ttatatactt ttgcaactta tcgaacagtt gatatctatt atcgatccgc aattgcagaa
      121 gcaatcgctc ttacgattat cccagctgtt ttatattatg cttatggatt gatattcaat
      181 agagaaaaaa aagtcatcgc tttatctgtc agtataagtc tacttgtgta tactcatgtc
      241 ttaactacac tattgtgtag tgcattgatt gcttgtatga ttatagttcg tttgttttta
      301 agcaagcaaa taaaaaacga tataaaattt gtggtcgtga atttagtaaa ggctgttttt
      361 ttgacttgtg cattaactag ctatttttgg tatcctatgt tgcagcagat gaattatcaa
      421 gaaatacaca gtcccttcaa aacagatttg cagctagaag cactttctat ttcagatagt
      481 atcattcaag cgttaaataa cgatattaca acatacacta tggggttatt aggattggtt
      541 gcgttgattt ttcctttatt catactgaaa gaaatggaga aaaaagaaaa aatcatctat
      601 gttggcgtag tttctacatg ggtatgcgta actaatttat ttccatggtt tttgtttcaa
      661 aaaacaccga taagcttgct ccagtttcct tggcgtatct taggtatcca aatctttttt
      721 tcttcaatga tcataacgct tgtttttatg aaaaagacta tgaataaaaa atattcatgg
      781 ttaatcattg gcgctactgt ggtgtttcta ctcgtcacgt ctatagcagc aaaagaaaat
      841 tacgcaaaag ttatccaatc aaaacacggt catattgtta tcaatgaaga aacactccca
      901 cgttatacaa catcagatat gggaggatta tacgattacg ccccaacaga agcgataaaa
      961 gaaaggcctc acttagaaaa acatgaagta aaagtaggag aatcgtggga aaaagggaag
     1021 tataaagctg cagataataa cattacttac acagtggcaa gcaacaaaaa gcaaaaagtg
     1081 acagtaccag tatatgctta tttaggtaca caggtaaaag taaacggtaa agtcgtaaat
     1141 gactcttata aaaaacatgg cttagtgaat gtggatgtaa atgctggaga aaataagata
     1201 gaaattacaa caaaatatac tccaactgct ctcatggctt tatggatcag tattctgact
     1261 tattgtttag tggtgtattc aagttttaaa cgatttgcac ctaataagtt gaaagattct
     1321 cgatatgtga ctaaaaagga gataaacaag aagaatgaaa ataaataaaa aagtagtaat
     1381 attgagtgga ttaattatag ttagtagtat atattctttc atctttttca aaaatacaca
     1441 gagtatttat accactggag atctttcttt tcacttatct cgaatcaagg ggttatcgac
     1501 tatttttacc agtccgatta attatgagac attcaattat actggatatg gtgtaaatta
     1561 tttttatccc tttctgactt tttttccagc tgtaatgtta tattggatga caaaaaattt
     1621 gattgtaagc tatattattt acgtttggtt attgaacttg tgtacaacac tcgtagctta
     1681 tcattatggc gagcggtttt taaaacaaaa aaaagcggca tttttattta gttgtctcta
     1741 tattttctct gcttatcgga cagtagacat ctactatcgt tccgcaattg ccgaagctat
     1801 cgcaataaca cttgttatcc ctgtattatt ttatgcttat caaatcatct ctggaaaaga
     1861 agaaaagtat ccgtctgtaa agctagcatt gagtatgagt ttgctagtgt acagccatgt
     1921 actatcaaca ttaatgagta cagcattaat tatcattttt atttttattc gtttggtttc
     1981 taaaggtttc aagaatgcag attttattgc tatatttaaa aaattattta gtgcggcagg
     2041 aatgacattg gttctgacga gtgctttttg gtatccgatg tttgaacaaa tgctctacca
     2101 aaaaatcaac aaacctagcg tgacaaattt atatgctcat gcatctaatg tgttcgatag
     2161 tctgactgaa gctatgaaca acgacttaac tacttactca atgggactag ttggtctgtt
     2221 atctttatgt attcccctga ttttatttaa aaaattaaca aacatagaaa aaaagattta
     2281 ttatggtacg tgtttgactt ggctagctac aactagtttg gtgccatggt atcttctgca
     2341 aaatacgcca gccaaattgt tacaatttcc ttggcggatc ttaagcttgc aaataatatt
     2401 ctcttctctg atattaacga tgatattttt caaaaataga agatacaata agacaagaga
     2461 actattttat ttatttatga ctgttttgtt attaatcact ttaactgttt ctagtaaaaa
     2521 aaatttcaat caaaaaataa tttctcagcc gggacatatt gttgtaaaca aagaaacagt
     2581 agaggcgtat accactaaag gaatggatta ttttttatat gattattctc ctatcgcttc
     2641 attggagaat aaggagcata taataaaaca cgaagcaaag attggcagtc aatgggtaaa
     2701 ccttccttat aaaacaactg gtagttctat catatataga gtaactaatg agaagagcca
     2761 agtaatcaca cttcctgtat atgcttattt aggaacgaat gtagaaataa atcatcaacc
     2821 tgtaaaggag caggtgaatg ctaatggatt ggtacaaata agcggagtca aaggagttgc
     2881 tgatattaaa gtaagcacaa gttatacagt aacgactatt atttcttatc ttgttacatt
     2941 aactgcatat ttatgtttat ttt
//
LOCUS       DANKHL010000252         2960 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000252 DANKHL010000000
VERSION     DANKHL010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.263"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(26..961)
                     /gene="murB"
                     /locus_tag="PW101_002675"
     CDS             complement(26..961)
                     /gene="murB"
                     /locus_tag="PW101_002675"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDL2515044.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1034..1954)
                     /locus_tag="PW101_002676"
     CDS             complement(1034..1954)
                     /locus_tag="PW101_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2515045.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2067..2819
                     /gene="xth"
                     /locus_tag="PW101_002677"
     CDS             2067..2819
                     /gene="xth"
                     /locus_tag="PW101_002677"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDL2515046.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          831 a          596 c          513 g         1020 t
ORIGIN      
        1 tacttatttt aagttccatt acttattaat tggctaaact tctgcttgtt tctgatattg
       61 cttgtttttc tccaatgatc cgcacttctg tttctaaaga cacatcaaat ttttctttga
      121 ttacttcttg gatgtgcgca atcaattcaa tataatctgt cgctgtcgca tgatcgatat
      181 tgacgatgaa tccagcatgt ttttcagaga cttgtgcacc gccccatttc aggccttgca
      241 atcctgcttg ttggatcagc tgacctgtat agtgtcctac tgggcgctta aagacacttc
      301 cgcatgaagg gtattctaat ggctgttttg attgacgcaa ttcggtcaac tcatccattc
      361 ttgctttgat tgcttcataa tcccctgttt gcaaactgaa tcgagcttcc aatacgatag
      421 cacgtaattc ttggacttta ctgtgacgat aagaaaatac catttcgtct tttgtcaaag
      481 ttttcaatgt tccatcttca agcaaaagat ccacttcagc aaaaacatct ttgatctctc
      541 cgccatacgc acctgcattc atataaaccg ctccaccgac acttcccggg atgccgcaag
      601 caaattcaaa tccagtcaat gattctgcca aagctgcata agtcgtatca atcagtgagg
      661 cacctgcttc cgcaatgacc atcgttcctt tgacatgaat ctgtttcatt tcagtcagca
      721 tgatgactac accgcgaatc ccgccatctt gaacgatcag attgcttgca tttcctaata
      781 ccatccaagg tatatcatgg attcggcaat aatcgatgat ttgctttact tcttcttgtt
      841 ttttcgggaa tgcaaggaca tcagctggtc cacctgtttt cgtaaacgta tagttcatca
      901 atggttcatc aaacaatata gtaatcgtgg ctaagctttc aactatttct tctttattca
      961 ctgaggattt tcctttcttt tctcaaacgt cctcattatt ctatcacgct taatacaatc
     1021 ttgccagtct tgttcaaagt tgatgacggt agtgatcttt aagcacttcc tcacataatt
     1081 catgacttct tacaacttct ttttgtttaa gcaattctgt ttccgattta cctaaacgga
     1141 tctgagaaaa gaaggcctct accattggct caaacccgcg tttgactagc gtgtttgtcc
     1201 agtcgccaaa ttctttcact ttttttccat cagcatcttg aatgacaagc tgggtcagat
     1261 cgtttacttc ataagtacca tttttacttg ttacttgata tttttctata ttagcgccac
     1321 ttgctaaatc catcgttaaa atagccgttt gattttgtgt ttctaattgt aaaatggcat
     1381 attccaaaaa ttcctttgtt tctcttatct ggtattttgt tctcaaaatg ggttcatcta
     1441 ataaatacac agctgtatca ataagatgta aaaacaaatc ataaaggacg aactctgtcg
     1501 tttcttttgc cgctattcga tttttttgca ggtggatcaa ccgcttatct gggatacgtt
     1561 tcaattcttc taccatagga gcaaagcgcc ggttgaaacc gatcatcaaa attatttggt
     1621 tcttatcagc taattcttgc aattcacgaa cttcatttag atggacactc agtggtttgt
     1681 caatataaac atggatacca ttttctagac attttttggc taattcataa tgtacttttg
     1741 ttgctgcgtg gatcatacat gcttcgattt tttcttctaa aagctcatcg atcgtttcat
     1801 atagatgttg gaacccgtac tctttttgaa ttctttcttt tgtttcttta tttcttgttg
     1861 caaaataaaa atcaagtgtt ccttgttttt tcgcataagt tggtaaatat gctttttctg
     1921 cgatattccc tacaccgatt acaccaatct tcatctactt tcctcctgga tcatttagtt
     1981 actttcatct tacaccattt tttactaaaa tgaggtacaa tagtatcgca aaggtaagga
     2041 aattaattcg aaggagtgtg cttttattga aatttatctc ttggaacgta aacgggcttc
     2101 gtgccatcgt aaataaaaat tttttagaag tttttcatga gttagacgcc gattttttct
     2161 gtctgcagga aacgaaactc caagcaggac aaattgattt agatcttcca ggttattatc
     2221 aatattggaa ttacgcggaa cgaaaaggct attccggcac agcgattttt gctaaaaaac
     2281 cagcattgaa tgccacttac ggaatgggta tcgatatcca tgatacagaa ggccgtctga
     2341 ttactttgga atattccgat ttctttcttg taacctgtta cacgccaaat tctcaaagcg
     2401 aattaaaacg tttagattac cgattagaat gggaagaagc cttttataat tatttggaaa
     2461 atctaaaaaa acaaaaacca gtcatcgtgt gtggggatct aaatgtcgct catcaaaaga
     2521 ttgatttgaa aaactggaaa acgaatcaaa aaaatgctgg attcactcca gaagaaagag
     2581 cggctctttc tcgactgttg gataacgggt ttatcgatac attccgttat ttttatccaa
     2641 cccaggaagg tgtctactct tggtggaact accgattcaa ttcaaggaaa aacaatgctg
     2701 gttggcggat cgattacttt ttgacgagca aagatttaga gccaggacta gctgatgcaa
     2761 agatccatac ggacattatg ggcagtgatc attgtccagt agaattagat ttgaaataaa
     2821 gaggttgtga aaaagtcgtt tagtttcgag tattaagaaa aattttgtga aaatggcttt
     2881 ccacattttg cataaatttg gcataatacc gagaaactgg cttttgaaca ctgtttatcg
     2941 agagattgtg aaaaagctgt
//
LOCUS       DANKHL010000253         2924 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000253 DANKHL010000000
VERSION     DANKHL010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.264"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(74..616)
                     /locus_tag="PW101_002678"
     CDS             complement(74..616)
                     /locus_tag="PW101_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDL2515047.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            751..1140
                     /locus_tag="PW101_002679"
     CDS             751..1140
                     /locus_tag="PW101_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515048.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1144..1908
                     /locus_tag="PW101_002680"
     CDS             1144..1908
                     /locus_tag="PW101_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2515049.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2037..>2924
                     /locus_tag="PW101_002681"
     CDS             2037..>2924
                     /locus_tag="PW101_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2515050.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKG"
BASE COUNT          942 a          491 c          600 g          891 t
ORIGIN      
        1 tcaaaataat aaataaaaac tgtctaacat ttaaaattta atttagatat tagacagttt
       61 ttaagtttgt gagttatttt ccttgtcgtt ttctcacttt tttagtccag aaaccacctg
      121 aaatatattt tggctcataa gaaatgataa aagcacgctc ttcgatttct ttgatcaggc
      181 gatacagttc acgttcgctt tttctaggag aaagtatttc gagaactagc cgctcacctt
      241 ccagccccat tgcagaagtc tgtgtcacac cataaccgtg ttctctcagt atacccggca
      301 aacttttatc ttctgcgcta tttgctggca aaatcgttgt gaccatgatg tatcctaatg
      361 ccaatttgtc ttcaatcaaa attccgacac tgatacctac tgcgtaacct aacgcataga
      421 caaataaatt cagtggatta tctaaacgat ctaaaacaag ggataagccg acgatataaa
      481 tcgtgatctc gaccatactg acaagtggag caatcagtcg atagcctttc attgtcaaca
      541 tgaaacgtaa cgtgttcaga gtgatataag caaaattaat gaggaagatc atccccaaca
      601 tttttggatc aatcatttgt aaaactcctt tcatttatat aacaacaata tcaaaaaaaa
      661 tcgtaagtga agaaaaattg aaactttata catacaaaaa aatgataaaa tgattataat
      721 agaattataa agataggtta ggtgattaaa atgaaaacgg tcaatcgtgt gttttgtgcc
      781 agccacgtgc taagtctcct tctcagtata tacttaggtg tctattcgat tcaacagcca
      841 attgaggtag agaacattgt ttttcagatg tcgctgacag atggtttaat tttaagcgtt
      901 tttttctttt tgatactgtt tattggtaat attttcggta gcgttgccag ttttttgaat
      961 ttttctattt atccactcct ttcgctagca ttaggagtgg tgggactttg ttctctgtgc
     1021 ttatttcccg agccattcag tcctgcattt atattatttg gtacactgaa cctgttccag
     1081 acaactgttg gagcatggtt gctatggcga tcaggaaatt taatgaaaat aggtgagtaa
     1141 caattgaatc caattatatt gaaacgttct gtccatcata ttccagtgtt agaagttgtt
     1201 ctgaaagaat taagagagga acgtattccc gttgtcatct attatcacgg atggcagaca
     1261 agtaaggagt tagttttgac gcaaggaaga aaaattgcgc agcagggcat tcgtgtgatc
     1321 ctgccagatg cgatgaatca tggggagcgc aaacagccgg tttcttctat tccatcctat
     1381 acattttggg atagtatcta cggaaatctg tttgaatttg acacacttat caagcattta
     1441 gaaaaaagag gtcttctaca tgatcagctt gcagttggcg gggtttcgat ggggggaatg
     1501 acaacatgcg ccttgttggc taaacatcct catatagtag cgggcgtgtg tttgatgggt
     1561 tcccctgcac ctttggaata tggaaaagaa atcgtaaggc gcgcatcgga atataattat
     1621 gtcgtaccaa aagactattt tgatttgatt tcatgggtta agagctatga tttgtcattg
     1681 cagccggaaa agttagctgg acgtcctctg ttcttttggc atggacaagc tgacgaaaaa
     1741 atcccttata tacaaacgga acgatttgta gaaaaaaatc ggcataagtc ttacggaaaa
     1801 gaaattgttt ttcgttcctc aaaaacacag cgacatatgg tacaaatacc actaatggaa
     1861 gaagcttcca cttttttagc aaaaaatttg ataaaaaagt ataaataatt gaaacggaaa
     1921 atcactcatg acgatagagg attttccgtt ttttattttg ggctataata aactctgtta
     1981 ggaaaatttg gtataattga tagtaagaat gacaacatgt tagaaaggaa gagagcatgc
     2041 ccactttagc agatgttgca aaacgtgcca atgtttcaaa aatgacggtc tctcgagtca
     2101 tcaatcatcc ggaacaggtg actgacgaat tgaaagagtt agttttctca gcaatggctg
     2161 aactggatta ccgtccaaat attgctgcaa aagcacttgt cacgaatcgg tcgcagatca
     2221 tcaaattatt tatactggaa gaaatcgaca caactgagcc ctattacatg aatttgctga
     2281 tgggaatcgc taaacggatc ggtaaagcgc attattctct ccaattagtt acaaatgatg
     2341 ccttcgacgt cggttcttgt gatggataca tcatcacagg tgtcagaaaa aaagacttcg
     2401 attggatcga ccgtttggaa aaaccagttg tcttgtttgg ggaaaattct gaaggatatg
     2461 attatgtaga cagcgataat gaatatggta cagcaaaagc aacgcaatat gccttagaaa
     2521 gagattatca aacgattatt tatatcggta tcgaagtgga cgagcctttt gaaatttcaa
     2581 gggaaactgg ttatttatct gtgatgaagg aagttgatct tcctagtaaa atcttgcggt
     2641 ttccaaatca ttcgacagaa gtagaaagat atatgacaga aaactgggca gattttacag
     2701 ataaaacttg ttttgtatgt agttcagatc ggctagcact aggagcagta agagggatta
     2761 taaatgctgg aggacacttg cccgaagaat ttggtgtgat tggatttgat ggggtatttt
     2821 tagaccaaat cagttcacct aaattgacaa cgatcaagca agatattata cgtatgggtg
     2881 aagtatgtgg agaaatgttg ttaaaaaaaa tagaagaaaa aggg
//
LOCUS       DANKHL010000254         2860 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000254 DANKHL010000000
VERSION     DANKHL010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.265"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..456
                     /locus_tag="PW101_002682"
     CDS             <1..456
                     /locus_tag="PW101_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HDL2515051.1"
                     /translation="IKGKEEKEKLAVVITIRPTDTEPFTNDLSMLLIEKNIRQLGFTG
                     FIAVNLFSYTKAKSPRVFSRGNDEQSLEILTTIFTEKKINTIIFACGSITATSQVAYE
                     QAKTIYDQLSAKQKKMTKVLINAEGKLSHPLSIHVRNEWQLSDHSLLFQ"
     gene            612..908
                     /locus_tag="PW101_002683"
     CDS             612..908
                     /locus_tag="PW101_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515052.1"
                     /translation="MKVDGECLTSSYKSIYEFFEEKGLDTDLLYQFYIAFRGQQLSFP
                     MKMYDRELVAQKIETMIQENHSIDSRKLTDRYGFSTRWIQSIIRQQQKEQHVHG"
     gene            901..1791
                     /locus_tag="PW101_002684"
     CDS             901..1791
                     /locus_tag="PW101_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HDL2515053.1"
                     /translation="MANEETVGLRIIEEAFKLPMVKVDRRAFLIQTFQGNVSDIEGLI
                     EKGPQAFLSKEMLDREAQKCIQSITLKSSAVSLATGLPGGIAMAATIPADLLQFFGYS
                     LRLAQEISYIYGFKDLMGETGEFSEEAKNTLILYLGIMLGVTSAGSAVRAMSGKLSTQ
                     ALKKIPQKALTQTLYYPVIKRVLGIFGTKLTKNTFAKGISKAVPVVGGVVSGGINYLS
                     MKPMATKLQTELGKNVAYTEKNLQQDLDILEGEFEEVSSDIVSSDTVLSQLERLSHLL
                     ETNMITEEEFQQLKQELLKK"
     gene            1873..>2860
                     /locus_tag="PW101_002685"
     CDS             1873..>2860
                     /locus_tag="PW101_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDL2515054.1"
                     /translation="MKAKRLFLLGTILLLGGCSTVSQNEQKKQVDSTTYETETTRKES
                     STKEIETTTQETKTTESSSKATSSDTSDFQIKQELWDTNKASQLATFVPQWGKTLGQE
                     YKSYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGNAGFNLVAVYSDV
                     ETGEYLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFTDIINGQT
                     PQTPVVEESTQQATTTTTQSTSSSGISNWEAAKETVLNNKALWIYESPDKNEANTNII
                     ESTQYRKDLMQDENGEFYPVDISTDDIGNTVMNGGKQFRVYTDGRISQRIGMT"
BASE COUNT         1060 a          426 c          551 g          823 t
ORIGIN      
        1 atcaaaggaa aagaagagaa agaaaaatta gcagtagtca ttaccattcg tccaacagat
       61 acagaaccat ttacgaatga tttgagtatg ttattaatcg aaaagaatat ccgacaattg
      121 ggatttactg gttttattgc cgtgaatctt ttttcgtata caaaagcgaa aagtcctcgg
      181 gtattttctc gtggcaatga tgaacaatct ttggaaatat taacgaccat ttttacagaa
      241 aagaaaatca acactattat ttttgcttgt ggttcgatta cggcaacgag tcaagtggct
      301 tatgaacaag caaagaccat ttacgatcaa ttgagtgcga aacaaaagaa gatgaccaaa
      361 gtattgataa atgcagaagg caagttatct cacccattaa gtattcatgt tcgtaatgaa
      421 tggcaattgt cggatcattc tttgttattt caataaacga aaataagtat tcaatgtata
      481 tgtttctttt tctgttattt tacttgttct tttgcagact aaacaacttg taaaacctct
      541 tgttatatgc attatcaatg aaggaatcaa tatgatattt ttaaaatgtc aggggaaatg
      601 gaggtgagca agtgaaagta gatggggaat gtttgacgag ttcgtataaa agtatctatg
      661 aattttttga ggaaaaagga ctagacacgg atttacttta tcagttttat atcgcttttc
      721 gtgggcaaca gttgtcgttt ccaatgaaaa tgtatgaccg agaattagtc gctcaaaaaa
      781 ttgaaacaat gatacaagaa aaccattcca ttgattctag gaaattaacg gatcggtatg
      841 gtttttcaac gagatggatt caaagtatta tcagacaaca acaaaaggag caacatgtac
      901 atggctaatg aagaaacagt aggtttgaga atcatagaag aagcattcaa attaccaatg
      961 gtcaaagttg atcgtcgagc atttttgatt caaacatttc aagggaatgt ttctgatatt
     1021 gaaggactca tagaaaaggg tccacaagca tttttatcca aagaaatgct agatcgtgaa
     1081 gcacaaaaat gtattcaatc gattaccttg aaatcaagtg cggtatcgtt agcaactgga
     1141 ctgccaggag gtatcgctat ggcagcaact attccagcgg atctccttca gttctttggt
     1201 tattcattac gattagcaca ggaaatttcg tatatttatg gatttaagga tttaatggga
     1261 gagactggag agttcagtga agaagcaaag aatacgctga tattgtattt gggaattatg
     1321 cttggagtaa cttctgcagg ttctgccgta cgagcgatgt ctggaaagct gtctacacaa
     1381 gcattgaaaa agattcctca aaaagcattg acacaaacgc tgtattatcc agtaatcaaa
     1441 cgagtactag gtatttttgg tacaaagtta actaaaaata cctttgcgaa aggtatttct
     1501 aaagcagtac cagttgtggg aggcgttgtt tcaggaggca tcaattactt gtctatgaaa
     1561 ccaatggcaa cgaagcttca aacagagtta gggaaaaatg tagcgtatac agaaaaaaat
     1621 ctccaacaag atttagatat tttagaaggt gaatttgaag aagtatcttc tgatatcgtg
     1681 tcttctgata cagttctttc tcaattagaa agactttctc acttgttgga aacgaatatg
     1741 attacagaag aagagtttca acaattgaag caagagctgt taaagaaata attatttaga
     1801 aaaagcaacc acttatacgg gtggttcttt ttcttgaatg aattcaaatt tattattagg
     1861 agaagaaaaa ggatgaaagc aaaacgatta ttcctattag gcacgattct tttacttgga
     1921 ggatgttcta ctgtctctca aaatgaacaa aagaaacaag tggattcaac aacttatgaa
     1981 actgaaacaa caagaaagga atcttctaca aaagaaatag aaacaactac tcaagaaact
     2041 aaaacaacag agtcttctag taaggcaacg agttcagata cgagtgattt tcaaataaaa
     2101 caagaattat gggatacaaa taaagcatct caattagcaa cttttgtccc acaatgggga
     2161 aagacattag gtcaagaata taaaagctac aatcctcaga acaatgtaag tctgtatggt
     2221 acgcctttac caagtgcagt tattgatgga aattggaaaa tggcaatcaa tgaagcacca
     2281 gttactgtac aatggtcaga agatggaact ggaaatgcag gatttaattt agtagcagtt
     2341 tattctgatg tagaaactgg agaatattta gggcagcatg tttacttttt cggttttcaa
     2401 aatggacaac ctaaagttta tttaacgcaa caaaatcaag gtaatgaaaa taattatctg
     2461 tactttaacg aaactcaaaa tcaacagcta aaacaaggat ttacagatat cattaatgga
     2521 caaactcccc aaacaccagt agtagaagaa tctactcaac aagcaacaac tacaacgaca
     2581 caatctacaa gtagttcagg aatttcaaat tgggaagctg ctaaagaaac tgtactaaat
     2641 aataaagcgt tatggattta tgagtctcca gataaaaatg aagcaaatac taatattatt
     2701 gaatccaccc aatatagaaa agatttaatg caagatgaaa atggagaatt ttatccagtt
     2761 gatatttcta ctgatgatat tggaaatacg gtaatgaatg gaggaaaaca attccgtgtt
     2821 tatacagatg gaagaatttc tcaacgtatt ggaatgacta
//
LOCUS       DANKHL010000255         2832 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000255 DANKHL010000000
VERSION     DANKHL010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.266"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            39..1517
                     /locus_tag="PW101_002686"
     CDS             39..1517
                     /locus_tag="PW101_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HDL2515055.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRLESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(1585..2676)
                     /locus_tag="PW101_002687"
     CDS             complement(1585..2676)
                     /locus_tag="PW101_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HDL2515056.1"
                     /translation="MLELNTSLSNRTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFIFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
BASE COUNT          986 a          511 c          453 g          882 t
ORIGIN      
        1 ttgtttgtta ttattagcaa caaaaaaagg agtgcatcat gaaaaagtta tctcaaaaac
       61 gtttagctca aatcattatt gattctagaa aatctctgaa aatgacacaa caacaacttg
      121 cagtagctac tggtatcaac cgtgccttaa ttagtcgatt agaatctctc gattttatcc
      181 cgtcaataca acaattagaa tcattagcag aagttctaaa ttttgaacca acagatctat
      241 ttataaatga atcaaataaa tcaaacagaa caaaacatca gtcgaagaat attgctgtag
      301 ccggtactgg ttacgtaggt ctttccattg ctactcttct tgcccagcac aatcaggtaa
      361 cagcagtaga cattcttcct gaaaaagtaa aacaaattaa caataagaaa tctccaatac
      421 aagacgatta cattgaaaaa tatctagctg aaaaaaatct taatttaatt gcaacgactg
      481 atggtgcagc cgcctataga gaagccgatt ttgttgtcat tgctgctcca acaaattatg
      541 atagtaaaca aaattatttt gatacttctg ctgtagagtc agtaatcaaa ttggtgttag
      601 aagtcaatcc aaaggcaata atggtcatca agtcgacggt acccgtaggc tataccgaaa
      661 gtattcgtaa aaaatacaat actaaaaata ttctattcag cccagaattt ctcagagaat
      721 ctaaggcatt atatgacaat ctatatcctt cgcgaattat tgtcggcacg gaccaaactg
      781 acaaaacttt acgagaagcc tctcacactt ttgcagattt actccaagaa ggagcaatca
      841 aaaaagatat cgaaactctt ttcatgggat tcacagaagc tgaagcagtt aaattattcg
      901 ctaatactta ccttgcactt cgagtatctt acttcaatga gctggatact tatgcagaaa
      961 tgaaaggact aaatacacaa gaaatcattc acggggtttg cttagatcca agaattggca
     1021 cacattataa taatccatct tttggatatg gaggctattg tttaccgaaa gatacaaaac
     1081 aacttctcac taactataac gatgttcctc agaatatgat aagtgcaatt gtcgatagta
     1141 atcgtacaag aaaagacttt atagcagatc gagtgttaga acttgctggc acgtatgagg
     1201 ctaacagttc gtggaatgca gaaaaagaaa aagaggtagt cataggtgta taccgtctta
     1261 caatgaagag taattcagac aacttccgtc agtcatctat tcaaggagtt atgaagcgaa
     1321 ttaaagcaaa gggggcaact attgtaattt atgaaccaac acttgaaaat aacactacat
     1381 tttttggttc aaaagtaata aataatttag atgattttaa aaatatcagt cacagtatta
     1441 ttgctaatcg ttacgatagt aatcttgatg atgtaaaaga aaaagtatat accagagatt
     1501 tatttcaacg agattaaaaa ataacacctc agatttgtag tgtctgaggt gtttctataa
     1561 acttttataa gtaaaaaaat acaattatac ttcataaaaa gacttatacc actgcgcaaa
     1621 tttacgaagc ccgtcacgca aacttgtact tggtttaaat tcaaaatctc gttctaacgc
     1681 actagtatct gcataagtaa ctggcacatc tcctggttgc attggaacta gttcggtatg
     1741 agattcaaag tcgtaatctt ctggtagcac ccctgctgca attagctctt gttgtagaat
     1801 atctacaaaa tctagtaggt tttccggcgt attgttacca atattataaa tatgatatgg
     1861 tgggattggt agcccatctt ccccatcaat tttctctgga gcgcactgca taaccctctt
     1921 tactccttca acaatatcat ctatataggt aaagtcacgc ttgcaattcc cataattgaa
     1981 tatttggata ttatcaccag cacgaagttt atcagtaaaa ccaaaataag ccatatctgg
     2041 acgcccagca ggaccatata ctgtaaagaa acgtaaccct gtagacggaa tattgtaaag
     2101 tttagaatat gcatgtgcca ttgattcatt acttttctta gttgctgcat acaacgatac
     2161 ttgattgtct actttatcct ctgtagaata aggaattttc ttattagtac cataaacgga
     2221 agacgatgag gcatacacta aatgttccac accagattct ccattatcat atgaatgacg
     2281 acaagcttca agaatattat agaatcctat tatgttagat tcgatataca tatctggatt
     2341 agtaattgaa taacggactc ctgcctgagc tgctaaattc accacgatac ttggtttata
     2401 attatcaaag atgaaatcaa ttgttgcttt atcagcaata gaacctttga taaaattcca
     2461 agtagatcct ggatggttct ttgcctccgt ttctatctct gataatctat agttcttaat
     2521 agagacatca tagtaatcat tcatattgtc aatgccaatg atttttattg atgactgagt
     2581 acgtaataac tcaagaacca aatttgatcc aataaaaccg gctgcgccag ttattaatat
     2641 tgttctattt gataaggacg tgtttaattc cagcattttt tagcactcac tttctttttt
     2701 tcttatgaat acatatttgt tatatattaa aaaatcttat ttagacttat ctattgtcac
     2761 ttttatcatt ctaatctttg cacgtagtag aataattata cagataggaa attgctaagc
     2821 aacccacttc tt
//
LOCUS       DANKHL010000256         2784 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000256 DANKHL010000000
VERSION     DANKHL010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.267"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            126..572
                     /locus_tag="PW101_002688"
     CDS             126..572
                     /locus_tag="PW101_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2515057.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            845..2197
                     /locus_tag="PW101_002689"
     CDS             845..2197
                     /locus_tag="PW101_002689"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HDL2515058.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            2368..2655
                     /locus_tag="PW101_002690"
     CDS             2368..2655
                     /locus_tag="PW101_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515059.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT          978 a          456 c          549 g          801 t
ORIGIN      
        1 aacgcgactg ttttacgtct tttttcatta aaatacaata aaattgaatt ttcttttcat
       61 gttaagtgga ttttaaaatg taaaaatagt atgataagac gagaataaca acgtggggtg
      121 tttacatgga aaataagtta gtcgaagagg ttatcgaaaa gttaaaaaaa gagaacattc
      181 gaatcacacc tcaacggtac gcggttttag aatatttgat tgaacatcat tcccatccaa
      241 cggctgatga gatttataaa gcacttgaac atcggtttcc aaatatgagt gttgcgactg
      301 tttacaataa ccttcgctta tttacttcga ttggatttgt tcaagaaatg aagtacggcg
      361 atgcttctag ccgatttgat ttcagttcaa agcgtcatta tcatgtaatt tgtgaaaact
      421 gcggtaagat tgttgatttt cattacccag ggcttgaaga tatagaaatg gctgcaagca
      481 gactgactga ttttgagatt aatgaacatc ggttggaatt gtatggactc tgtccggatt
      541 gtaaagagtc agcaaaaaag gcgcaaatgt aaaaacagtt tctacaagtg ttatacctaa
      601 aaaaagctag ctgtgtaaaa aaaacgctag cttttttatt tttttgtctt ttattcacaa
      661 tcttagatat aaaagtgaat attattaaaa taagaatctt gtagatattg ggttcttatg
      721 cttgaaaagg aatgaaacta caaacaaacc tttgtgaatt cacaagcatt aaactgttgt
      781 atccgtttta tttctgttat agtagttttg tcgaaggaca aaatgttaat cggagggacc
      841 ataaatgaaa gtagtagtaa ttggctgtac tcatgcaggt actgcagctg taaaaagtat
      901 tttaaaaaat catccagaag cagaagtgac agtatatgaa agaaatgaca atatttcttt
      961 cttatcttgc ggtatcgcac tttatgtcgg cggagtagta aaggacccag caggcttgtt
     1021 ctactcaaat cctgaagaat tatcttcatt aggagcaaca gttaaaatgg aacacgatgt
     1081 aacagacgtc gatacagaaa ataaaacagt aacggcaaaa gatctgaaaa caggagaaac
     1141 agaaacggtt tcttatgata aattagtgat gacaactggt tcatggccta ttatcccacc
     1201 gattagtgga atcgatgcga aaaatgtact cctatgcaaa aactacaatc aagcaaatga
     1261 aattattgca caagcgaaag aagcaaaacg tgtagtaatt gttggtggtg gatatatcgg
     1321 tattgaatta gtagaagctt ttgtcgaatc tggaaaacaa gtgacattga ttgatggatt
     1381 agatcgcatc ttgaataaat atctagataa accattcaca gatatcttag aaaaagaatt
     1441 gacagaccgc ggtgttactc ttgcgctagg cgaaaatgtt actgaattta tcacagatga
     1501 agaaggaaaa gtgaaacaag tagctacacc aacagataca tttgatacag atatggtcat
     1561 tttatgtgtg ggcttccgtc caaatacaaa actgatcgaa ggaaaagtag atacactgcc
     1621 aaacggggca atcaaagtga atgaatacat gcagacaagt aatccagaca tttttgcagc
     1681 tggagactct gcagtagtca actacaatcc aagtggcacg caaaactata ttcctttggc
     1741 aacaaatgcc gttcgtcaag gtttgcttgt gggaaataac ttgacagatc ataaaatggc
     1801 gtatcgtggt actcaaggaa cttcaggact ttacctattt ggctggacga tcggttcgac
     1861 aggtgtgacc aaagaaagcg caactttgaa cgatctagaa gtccaagcga ctgtatttga
     1921 agataattat cgtccagagt tcatgccaac tactgaaaaa gtaatgatgg agctagtcta
     1981 tgaaaaagga acgaatcgta tcgtaggtgc tcagttcatg tccaaatacg acattacaca
     2041 atcagcaaat accatgtctt tagctgttca aaataaaatg actgtagaag atctcgcact
     2101 ttcagatttc ttcttccaac cacattttga ccgcccatgg aattacttga acctacttgc
     2161 acaagcagcg ctaggcgaaa tcgaaactgc acaataaaga aaaagggttg tgacacaatt
     2221 ttgtcacgac cctctgtctt tttttgtgaa aagtacaatt agttgtcttt cttcgaattt
     2281 tttgtcacaa taagcagcaa agataaagct gttacataaa agaacaagca gctttatttg
     2341 attttgaaaa gaaaggtgag aaagaagatg aataaagaat attttgtcca acaagatgca
     2401 tttttagaag attataaaag tgcttcgaag aaattaacga tcaatattga tgagttggca
     2461 gatcaagtac tctctcagtt gaatgaaacg caagaggaat attacacgat gccagcaaat
     2521 gacgccaaag ataatgtttc tcatcgtttt cctttcagta gacgcatcta ctcagatgaa
     2581 aatgacggca cattgaatac agagtacaca tatgaaggtc agccatatgt gctagaaact
     2641 gatgatgatc tttaaagaca gtttttgaaa aggccgctca tccttaagtg atgacaaaaa
     2701 atcatcacaa ttaccgatga ctgtctcttt actatctttt aatcagaaaa aggggttatg
     2761 acaagctttg tcaacccctt tttt
//
LOCUS       DANKHL010000257         2778 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000257 DANKHL010000000
VERSION     DANKHL010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.268"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(502..1254)
                     /locus_tag="PW101_002691"
     CDS             complement(502..1254)
                     /locus_tag="PW101_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2515060.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(1269..2516)
                     /locus_tag="PW101_002692"
     CDS             complement(1269..2516)
                     /locus_tag="PW101_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2515061.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(2592..2738)
                     /locus_tag="PW101_002693"
     CDS             complement(2592..2738)
                     /locus_tag="PW101_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HDL2515062.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
BASE COUNT          961 a          464 c          341 g         1012 t
ORIGIN      
        1 caaaaggact tgcagcgctg tagttggtgc tgcaggagga cctatttcgg ccgttacagg
       61 aggtattggt aatgtacttg tcggatgtgc attaaagcct gttcctgcaa gttaaattta
      121 taaaaacagg aaagtaatta tctcaaatcc tatcaagtac aatcatagga ttgcacttga
      181 caggacaaat tttttaacta aaaaatgaaa aacactttct aaaaattagt agaatttgcc
      241 aaatctttcg catcttccac catacattta attttaattc ccctttatgg tacttaataa
      301 aaacaagaaa agaactattc atttggccct tttttggttc cagaccatag aaatattcct
      361 ccagtaacta atattgctgt tatgcacgta actaataaca gctataaaaa ttggtacaac
      421 tttaatagga ttcagaaaaa tttgcactag tgaaggaatc tattttatta gaaaaatgca
      481 atctccctaa agaaaaaacc actaaacagt gctttctttc aacttctcct taattttttg
      541 tgctttccct aaagtaaagt ttctggccaa ctctgaatca aaatatagtg aacgtttttt
      601 aaaatctatt tctttaacgt taaagatatt tactaaacac gctcgactaa ttcttgttaa
      661 cataggatat ttcttttcaa gctcactaat tcttccataa aattcgtact gcccatcttt
      721 agtacacaat gataatctat gtggtaattt tgaagcttct acaaaataaa tgtcatttat
      781 atcaaaagta aatgtttgag aacctatgga aaaaacaaat gcttgattcg taacctgttt
      841 tgttgcatct atcctttctt gcgctaataa caataactca acaatctcgt ttcgatactc
      901 atctagtgtt tgatcttttg ttacaaatcc taacgtttca actcttcttt ttattgttac
      961 gggtaacatc tcatcatgag tagttgtaaa aatgattttt gcttgaacat catattttct
     1021 gattacttct gctaattcta taccgttaac ttcatgattt aaatcaatat ctaagaaata
     1081 tattccttgc tttggtcgga attgtttgag gtatttttta acttctaacg gactttgtgt
     1141 tttcaatact atcttaaata ctttatcgtg aaaaagaatg aaattatcaa taattcgttc
     1201 taactgattt aattgtataa tttgatcttc acataatatt attggataac tcataaatcc
     1261 tccttccctc aaaacaacaa aataacttga gtagtaaatt gatacggatc gttttgatag
     1321 ttcacaaaca tattgggatt ctttttattg atttcctcta tactagataa acctaaccct
     1381 tgatgtccct tttttgttgt tattcctttt ttcattagag tatctataga gagtagtatc
     1441 tctctacaac tattcgatat cgaaatttct atctgtttat catctcggta aatagctaga
     1501 gacagttctc tatcttgact ctctatagat gcttcaattg cattatcgag aataattcca
     1561 agcaccctaa tacagtcaaa tattggaaca ggaacttcaa taatagtact tggacattcg
     1621 aatttacaac aaatcttatt gttatttgct tggtatagct tcgctatcaa caaacttttc
     1681 aaatattcat tttttatatt ttttaaataa cgatattcaa acttattggt tagataagaa
     1741 ttagaataga tctctaattc ttttatttct ttttccaata tcgaatcatg gttttgatta
     1801 gcgatttctt ttaaactcag caataaattt ttatagtcat gtctaaatct ggcaaatttt
     1861 tcttgatctt tttccagtga atcagtatat ttttttagat aaaccaattc ttttcttttc
     1921 aattgctgtt catattgttc tcgttgccta atagttattc taataaataa aaatagaaca
     1981 acaaacattt gaatgattaa aaatattaag acaccaaaca caaaatgatc aaatacttga
     2041 tattgatgag caacataaga aacaaataaa ccaattgtaa gtaaataaat catgaatata
     2101 gcagtaagtt tagaactgga ctgttcaaca atcctattta tagctaattt tctatataac
     2161 caaataaaca tcagcaaaaa caaagaatca agcattatct ggaatactac ataaccatac
     2221 ccttttatcc cttcaaatga aaacatatac gtcataacca tggaagtaat aatagatctg
     2281 aaatcatcaa taagcataca taatagtaat ttatttaaaa gcactatatt agttttcttc
     2341 ttttccttta atataaaata acttccgatt ataggtatag ctgcggaaac atcagtaaat
     2401 aattcaacaa aaaaagtgag tggtatcaat aaacatacaa aaagatattt tcttttgtta
     2461 agatcagtat tagtcatata aaataagaga aaaaagaaaa agtttcccac gaacattctt
     2521 aatagataag cttttgtatc gatagtaatc aagagatccc ctcctgattt taggatatca
     2581 taaaaaaaat tttagttaca ctttttaaat aatgaaaaaa cacattccac taaatttgat
     2641 gccggttttc cttgaggttt tgttccagca cctcctttaa tttttttaag ctcttgatct
     2701 gaacattgtt tagcatttag tctatttttt tcttccatta cgattccccc tacaaactat
     2761 aaatactttt atgtcact
//
LOCUS       DANKHL010000258         2778 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000258 DANKHL010000000
VERSION     DANKHL010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.269"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            79..879
                     /locus_tag="PW101_002694"
     CDS             79..879
                     /locus_tag="PW101_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HDL2515063.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            895..1773
                     /locus_tag="PW101_002695"
     CDS             895..1773
                     /locus_tag="PW101_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL2515064.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            1786..2766
                     /locus_tag="PW101_002696"
     CDS             1786..2766
                     /locus_tag="PW101_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDL2515065.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
BASE COUNT          929 a          469 c          590 g          790 t
ORIGIN      
        1 ctaactcttg acagataatc aaaaagaatt tatagttgtt accgtagtag ctactaaaaa
       61 taaagtgagg attaatcaat ggaaacgatt attcaaatca tgaaaaaata tgatttttca
      121 gagatggaga caagagtgta taccacacta ttagaaaaag gtaatcttac aggatatgaa
      181 gtaagtaaaa tctcaggtgt gccacgctcg aaagtatata atattttgga aaaactatta
      241 aaaaagaatt tgattgtcgt taataaatca gaacctaaac tttatcatgc catctcggca
      301 aatgagtttt tagagaaatt agaaaaaagt gttaaaaatg atctttcttt tcttaccaag
      361 aatctaggga tgatcaaaga aaaagatgaa gaagaaatgc tgtggaaagt tgatggtatc
      421 gagtatgttc tggataaagt agagcatttg gtgaaaaacg caaaagaaag tttgctcatt
      481 caagtttggc atgagaattt cacggattca ctactaaagg cattgcagca agctgaaaaa
      541 agagttgata agtttgtact gattttattc agttctaccc atgaatatga tcttccctta
      601 gaaaagtatt atattcatgg ttttgaaaca gacaaacttg ctgactttgg agcaagatgg
      661 atcaacatcg tcgccgatga gcaagaagtc gtgtttggta ccattaacga agaactgcaa
      721 tcaaccgatg tcacttggac aaaaaaccac gccatggtca atctagcaaa agaatatgtc
      781 aaacatgatg catatacact aaaagtgatt gcagaatctc ctgaagaact taaagcaaaa
      841 tatggtgagg gctttgaggg aatcagaaag atttactagt aggaggaatt ttttatgatt
      901 cggaatattt tgctgacact aattgtcgca atcaatctat attttattat ttactttcta
      961 agagaccttt tcaaaaacaa acaaaatttc aaagaagaac caggggatat gcgtctgttg
     1021 cccttcacgt catttatcat cttctttctt tcaacatttg gcgtttccga ctttgctatc
     1081 ggaaccgtcc tttatccaaa gttgaaatgg gtcagcatga aaaaactacc cggaacgttg
     1141 aatacccaat gtgtagtacc ggtagccgtc atggcattgt cttacatcac tgccatcaac
     1201 gtagggatca agacactagc tgtttgtatt atctgccaaa ttatcggtgc atatttagga
     1261 ccaagatttg tcgtgaagtt accagagaaa acgatcaaac tgtttgtcgg cataggattg
     1321 gtcattgctg cattgttgat ttttgcagga caaatgaact ggattccatc aaacggaaca
     1381 gccagtgaat tgtatggcgg aaaattaatc ttggcaggat ttttactctt tgtctatggt
     1441 gcattgaaca atatcggcat tggttcttat gcgttaacga tggtaactgt ctacttgcta
     1501 ggcttgaatc cagttgcagc ctttcctatt atgatggggg cttgtacctt ctcagtaccg
     1561 atcggcagtg ttcaattcat taaatttaat gaatacagca gaaaaatcac actttttact
     1621 tcaaccttcg gtgtattggg ggtattggtc gctgtattcc tagtcaaatc tttagatacc
     1681 tatatcctga aatggatcgt gattttggtc ttactataca gtgcatatac gatgttgggc
     1741 agtcaattaa aaaaagttac agcagctaat taaggagcga ttcaaatgtt atttcttaaa
     1801 aaagaagata ttatcaaaag tttttcaatg agagaagcca tcgatgcgga caaaaaagca
     1861 ctgtcattgt attcagcagg aaaggcaagt gtgcctcttc gcaccaatat cgatgtgcca
     1921 aaaagcaacg gacaaagctt atatatgcct gcttatgtag aaggtggcga aggcgcgcta
     1981 ggtgtcaaaa tcgtttccgt gtatcctgaa aacatcaaga aaaacttacc aagtgtacca
     2041 gcaacgatga tcgtattgga tccagaaacg ggtatggtat ccgcttgttt ggatggtact
     2101 tatttgacac aattaagaac aggcgctgtt caaggagcag caacagaact tcttgcaaga
     2161 gaagatgcta aaattggcgc gctgattgga accggcggac aagcacagtc acaattagaa
     2221 gcaatgttga ccgttcgtaa attagaagaa gttcggattt ttgatattga ttttgagcgt
     2281 gccagccaat ttgctgaaaa aatgatgcaa caattttctg taacgatgcg cccaacaaaa
     2341 accagtcaag aatgcgttga aggagcagac atcattacaa gtgtgaccac ttcaaaacgt
     2401 gcgacattct ctgctgagtg ggtcaaaaaa ggtgcgcaca tcaatggagt cggtgcttac
     2461 acaccggaaa tgtgtgaaat tccacgagaa ataatcaaag ccgcagatgt tgttattttt
     2521 gataccatgg acggtgttct aaaagaagct ggggacttca tttcaccgct gcaagatggg
     2581 tatattcaaa gagacagcta tcatggggaa ctaggccaac tgatcaatga agaattgatc
     2641 ggtcggacaa gtggagaaca aatcacgatc ttcaaaacag ttggttcggc tgtattagat
     2701 gtggtagttg cgacagaaat cgttaagaaa gctaaagaaa ataatttggg aaaattgatt
     2761 ttctaattaa aaaggctg
//
LOCUS       DANKHL010000259         2768 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000259 DANKHL010000000
VERSION     DANKHL010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.271"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(155..379)
                     /locus_tag="PW101_002697"
     CDS             complement(155..379)
                     /locus_tag="PW101_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515066.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(399..821)
                     /locus_tag="PW101_002698"
     CDS             complement(399..821)
                     /locus_tag="PW101_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDL2515067.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(963..1673)
                     /locus_tag="PW101_002699"
     CDS             complement(963..1673)
                     /locus_tag="PW101_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515068.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(1691..2188)
                     /locus_tag="PW101_002700"
     CDS             complement(1691..2188)
                     /locus_tag="PW101_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2515069.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(2225..2677)
                     /locus_tag="PW101_002701"
     CDS             complement(2225..2677)
                     /locus_tag="PW101_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2515070.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
BASE COUNT          718 a          560 c          442 g         1048 t
ORIGIN      
        1 caaaacataa gtaaacaaaa aattcggatt ttaagcaaaa tccgaacgtt ccatctgatt
       61 aaatcccttt atctcaaagt tcatgaacat tcactttgga ttacaagaat ttctttattg
      121 tgttaataaa gtttcttcat tctagtctca aaaatcaata gtgtgatttg attttaccat
      181 ttcgtttctg ttcttctttg atgaattctt ttgtatcgac aggattcaga ttatttagtc
      241 cgccaaaacc tgtatcgacc atttttcccc agccttttgt cttctttttt cctgatttga
      301 gattctttgc tcgaagcttc tcactatgtt ttgctcgccg atacttcaaa taaccttcta
      361 atcttcccat tagatccact gtttttcctc ctttttattc aatcagacga ctcagctttt
      421 cctataacaa aataattgcc ttcagggtca gaaaaattga aagtcaagat gccattgtta
      481 tctgtgattt ctcctggttc ttccatgttc tcgtagatag tttcaaattc atccgtaaaa
      541 aatactaaag aaggcggtgg tcctaatacc tctggtgaaa acttctgcac aaatactttt
      601 gggaatacgc ctaattctaa ttcttttgag atgctgagta caatactctt aaattcttct
      661 ggcaattcga ttgtttcaga aatttctgca ccaatttttt ctacaaagaa tcggcaaatc
      721 atctctacgt cattgacata aagcatcaca cgtgtttttt gtatcatcat ttgacccttc
      781 tttctatttg attttctttc ctgtctataa actgattcca ttatatcacg aaagcgcaaa
      841 caccttgtgt ttgatactat tcaagaaaaa taaagcaata gtttctagga ttgctttcct
      901 ccacaaactt tttttacaaa aaaaaaacgg caatgaatgc cgctcttctt atttcgattc
      961 ttttataatt ctgcttctga ttcatctttg gttagtgtgt gtctaacact agttacgcca
     1021 aatgtttgat cgattttttc cttcaacgct tcgattgcct gtgcgccacg tgccgcttgt
     1081 tcagcattga tggattcaat gaccagcacc gctgcttgtg tttcttccgt cagcatcgtt
     1141 ctttgggctt cacgccagtt gagacagcga cagatcgctc cttgttcatc atagtagatg
     1201 atttcttccg gaagtgccgg agcatcggaa tctgcaccta aaggcaggaa tgattcgcca
     1261 ccttgggctt ttcctaaatg cagatttcct gcgatctttt cgatatcttc tccgccgcaa
     1321 ggtacgccaa aagacaagga aatactatta tagatatcta caagcggatt gattgggaaa
     1381 aattcccgtt cttgatttgc acgtttcagt aaagcttcaa tagaagaacg tgcacctttt
     1441 ttagtcttga atgtggtaaa tgcctgacgc cactgagcaa tgacttcgtt ttggctgaat
     1501 gtttcttctg taaggtaatt tcttgcttct tttgctgcct cattcaataa ttgatgatac
     1561 gtttcttcac tttcttttgt tacgtggtta tcgataccat ttacggataa gatttcgatg
     1621 gttgcttcag gaaatacttc ccagaaattg gtatccacta ttactttttt catctgttca
     1681 cacctcttga ttagaataat tgcttgatga atagatagtc tgtttgggga tcatctccca
     1741 tataaaaaac atgttcgctg tatttgataa atcctttttt acgataaaaa gcgagcgcaa
     1801 catcgttgtg ttcccaaaca ccgagccaaa tggctgtttt ttgcttttct tttgcttttt
     1861 caatagctgt atctagtaaa aatgtaccga taccttgatg tttaaatttt tttcgtacat
     1921 agattcgctc gatttctaaa aaatgatcag ccatttcctc agattgactc gttcctgtat
     1981 ttagtttcag gtaacccgca ggctcttgat cgatactcac taggaaaaaa tcggtttctt
     2041 tatcttttag ctcttctgta agtttttcta gtgcataagc ttgtgtcaag taatcagcta
     2101 aatgctccgg tgaattatct tttccaaatg tatctgtaaa tgtttcgata ctaatttgtt
     2161 gtagtaagtc tacatctgaa ggcgtcattt ttcgaatttc gtatttattc acggcttctt
     2221 cctttcaata gatcctttta ttgccttttt tgacaaaatt ccaatcttcg ctaacattct
     2281 tgcatacttt atttaaaaga tattccaatt gttttgcttc ttcgtcagaa agaccattta
     2341 atgcgactgc attggagtag ttgttttctc gaatgatgaa aggatagatt tctgctcctt
     2401 ttttagtcgg atagagtttc ttgatttttt tattttcttt atcttccaaa cgttcgatca
     2461 tcccattgga ttctaatttc ttgattgctc gggcagtcgt tgtacgatct actttgatca
     2521 tttctgctag tttttcttgg atgattccag gattttcgca aatacgtact aaataaagat
     2581 actgtcctct tgttaaatcg acttctttga attcgatatt gctaatagaa tctagcgctc
     2641 gggcaatcat tccgatgtct cgaaggattt cagccatttc atcccacctt tattttgttt
     2701 gtaagactag ttcacctctt tttgttgcaa atgcaactat tttcaataaa aaaatcaagt
     2761 tctttttt
//
LOCUS       DANKHL010000260         2689 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000260 DANKHL010000000
VERSION     DANKHL010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.272"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(34..1128)
                     /locus_tag="PW101_002702"
     CDS             complement(34..1128)
                     /locus_tag="PW101_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL2515071.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1317..2078
                     /locus_tag="PW101_002703"
     CDS             1317..2078
                     /locus_tag="PW101_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2515072.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2193..2633)
                     /gene="fabZ"
                     /locus_tag="PW101_002704"
     CDS             complement(2193..2633)
                     /gene="fabZ"
                     /locus_tag="PW101_002704"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL2515073.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          839 a          541 c          461 g          848 t
ORIGIN      
        1 ttctagcgga ttatttctgc aataactaat tatctatttt ttattttcaa ttaatttaaa
       61 gcttgaccat ggtttcaaaa agtcaaggag gaataattcg tcatcggaga ttcttcctac
      121 aacattgaca cgtccgtcgt ttttcatatc cttcaaggcg atttgtgttt ctcctttgta
      181 ttgaccgtac ccttcattgt caatcagtac atccccatga tgcatatctc gcgtattatg
      241 aggcgggaaa tctttatctt tgtagtatac acgagtcatc gttgaacgaa ggatatattc
      301 tgagcgatcc cctcgataac tatgaaggtt atcaaacaag cagattcgtt cgttttctgt
      361 aataccttct tccgtatcta ctttcaatgt tggataatcc gcgttaaatg cttctgccat
      421 ttctttcaat tcttcttcac ttgcgtacgc atttccaata gaaatatcat caatcaatcc
      481 tgtaaggatt aaatgcttca cttgtgtcgc aatttcaaga tctcggtggt cttccagcgt
      541 gcacaagcca tcttgcgtag gccaagggcc aaactcagca gattgcgaat tgacgaatgc
      601 catcgtattc aaattatatt ttctgaattt ttctgaacaa aaaacaaagt gttcgtatcc
      661 taaaccagag tatcgatgag gataaaaatt atgagaacct aataaattat ccgtgtttgg
      721 cgaataactc atgatgttgt caacgtaatt cgtgcccgaa ctcatattga tctcgatttt
      781 gataccaaaa ggattgcgcg tcatttttgc ttcttctgct cctgtgaatc cgatatctaa
      841 gcggaccccg taggcaccca tcttatggaa aaaagataag tcatcgtaag aaatctccaa
      901 ctgttcaaat aacgctggat tgatatcaac cattacttcc atgcctaggc tattcgcgta
      961 atcaactact tctttaaatt ctgaaagaac tttttcttta tcatcattga tctgcagcaa
     1021 actggtaaaa acccgtttat acccatactt atgagctaaa tccaagtacg ctttgtcctt
     1081 ttcaaatgtt gaccgttctg gataaatcga gattcctaat tttcccatat taccatttcc
     1141 tcctgcatct tttcgttttt gtatccactt acattatagc aaacacaaag taaatggtct
     1201 acaccttttc tttttctttt aaaataaatg ataaattcca ataatttaag taggtatttt
     1261 ccctctattt tgttatcatt aaagataaca aaatagtaaa ttggaaagga acagttatga
     1321 agaaaaataa agttatttta agtattagtt tagcagcagc attgctgttt gctgctggtt
     1381 gttctgcaaa caacacgact tcttctagtt cgtctacaga aagttcaaaa agtacatcta
     1441 ctgcggaaag cacgactgcc agtagcgtcg atttgaacgc attggaatta ccacaattga
     1501 cgaaagatgt cgccgaaaat gaagatctgg tcgaaatggt gacgaataaa ggcacaatca
     1561 aaatcaaatt atttcccgaa cacgcaccaa aagcggttga aaactttatg acacatgcaa
     1621 aagacggtta ctacaacgga ctgactttcc atcgcgtcat caaagatttc atgatccaag
     1681 gtggcgatcc taaaggcgat ggcactggag gggaaagcat ttggggagaa ggatttgaaa
     1741 cagagatcaa caaccacctt tataatataa gaggtgcgtt atctatggct cgctcacaag
     1801 atcctaacag taacggcagc cagttcttta tcgttcaaaa tacagatgat atgcatgatg
     1861 gtcttttaaa agacgactat cctcaagcaa tcatcgacgc ttataaaaac ggcggttatc
     1921 cttcactaga tggccattat actgtcttcg gccaagtgat tgaaggaatg gatgtcgtgg
     1981 atgctattgc aaatatcgaa acagatagtt cagacaaacc aaaagaagaa gtaaaaatcg
     2041 ataaaatcaa tatactgcaa gaagcaaaac aaaaataaaa aaataacgtc tgaatcctgc
     2101 atagatgaca acgtatgcta ggattcaggc gtcttctttt tacataaaaa gcaagtaagc
     2161 ttccttttgt tttgctatat caggttttat agtcaagcat tttggttgac gatgaacgtg
     2221 aactcgcact cacagacttt tttatcatct acatatgctg cagataacgc tgtaccgact
     2281 gatcccttca ttttggtgat ttcaaaatga catttcagca catctcctgg aacaactttt
     2341 tgacggaatt tggccttatt gatcccaccg atataagctc tttttcctag aaactcttct
     2401 gattttagga taagtatcga acctgcttgt gccagtgttt ctagaatcaa cgcacccggc
     2461 atcactggat tgtcaggaaa gtgaccttgg aaaaaatctt cattgattgt tacatttttt
     2521 gtcgcaacga tcttttttcc tggttctaaa gtatctacat aatcaatata acaaatagga
     2581 taacgattag gaatcaattc cattacttca gtagcagata atacgtaaga cataattaaa
     2641 ctccttcttt caggcaattg ccattttcat tactttgact atcaaagta
//
LOCUS       DANKHL010000261         2590 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000261 DANKHL010000000
VERSION     DANKHL010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.273"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            390..2483
                     /locus_tag="PW101_002705"
     CDS             390..2483
                     /locus_tag="PW101_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2515074.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT          849 a          446 c          510 g          785 t
ORIGIN      
        1 caatttcaaa aaacagcttc tcatgtttta taaatattgt gacttgtggc cgaggagttg
       61 gcaggagaac accgtttaag gaagaagttg tgaaaaagct gtcctgcatc aagaaataag
      121 gagctgcgac aagaattttg tcacagcctc tttttttgta cgaataccga gaaactggct
      181 tttgaatacc gtggagtgaa gcggtcacca caagactttt atctcttcat tagtttaatc
      241 gcttacatcg cagcggtttt atgctgtgaa aagtggacgg aataatcttt agaaagaata
      301 aaaaaatctt aagataacaa cttttttagg aaattttcat ttttcaggag caaaataaga
      361 aaaaatgtga atagagagaa gtgatatacg tgacacaact taaaaaattg tgccaaaatc
      421 gtttagcaat cgttttgaca gctgttttct ttttctggct gaagacgatc attgcctact
      481 atgcagattt ttcattaggt gtggaaggta cgatccagta ctttatcttg tggatcaacc
      541 caattgcgac taccttattg ttttttggcc tttctttgta tattaaaaag ccaaagccag
      601 cgttagctat tcttttgatc attgatattt taaatacgct tttgttgtac ttgaatatta
      661 ttttttatcg ggaattcaca gatttcatta cagtcaaatc tgtattaggc ttttcgaaag
      721 tgtcgcaagg attgtcagga agctcctttt ctttgatgaa gccccatgat gtcatctact
      781 ggctggatat cgctgtgttc attgggctac tggtttggtt gaaggtcaag aaaataccta
      841 tcaaaagcaa ccctgtcggc aaaccgatgg ctatcgctgt tacctgtctt tcagggctta
      901 ttttttcagg aaatctagcg ctgagcgaag ccaaccgtcc acaattgctg cagcgaacat
      961 ttgaccgttc atatattgtc aaatatctag gaatcgatgc gtatactatt tatgacggaa
     1021 tcaaaacagg aatgactagc agtgttcgcg cgcatgcaag cagtaatggg attgatgaag
     1081 ttttagatta tacaaagaaa cattatgcag agccaaatcc cgaaacattt gggatcgcta
     1141 aagggaaaaa tgtgatcgtt ctccatttag aaagtttcca gcagtttttg attaatatga
     1201 aggtagacgg acaagaagtc acaccgtttt taaatagtat tttccagaat caagcaacga
     1261 tcagctttga caatttcttt catgaagtag gacagggaaa gacaagtgat gctgaaaata
     1321 tgttggaaac tggcacattt ggtttacctc aaggctcatt attcacagaa cttgggtcag
     1381 acaatgtctt tcaagccgca ccagcgatcc ttggacaaaa gcaaggctat acaagcgctg
     1441 ttttccatgg gaatgtagct agtttttgga atagagatca tgtatataaa aacttaggtt
     1501 atgacaattt ctttgatcga tcttattttg atgaatccga cgaaacgtta ggttatggga
     1561 tattagataa agatttattc agagaatctg cccaatatct tgagcatcta caacagccgt
     1621 tttacacgaa atttctttcc gtaaccaacc acacgcctta ttatacggat gacaagaatt
     1681 tcgactttcc atctttgaat actggcaata gcacagtaga taattatgtt cgaacggcac
     1741 attatctgga tcaatcattg gaacaatttt ttacgtattt gaaaaaatct ggtatttacc
     1801 aaaattcaat ctttgtgatt tacggggatc attttgggat ctcgaataca gataataaag
     1861 atttagcaag cgctcttggt aaagatccag atacatggga tgaatttgac aacgcacaga
     1921 tgcaacgagt gccattgatg atccatatgc ctggttatac taaagggaca gtcaatcatg
     1981 aatacggtgg agaaatcgat gttttgccga cattactaca tctattggga atcgatgata
     2041 aagattatct tcattttggt acagacttat tatcttctca gcatgatcaa gtagttgctt
     2101 ttcgaaatgg gaactttgtg acacccaaat acaccgtcct aggaggaaaa gcttacaata
     2161 atcagacagg agaaatcatc gatacgaaag cagcaggaat cgatgaggaa atctccaaag
     2221 atcaggaaaa agtcaagact gcgttaagtc tttcagataa gctgaatcag gaaaatctct
     2281 tgcggtttta tgtgccaaac ggatttgaga cgattgatcc gaaaaaatat gactataaaa
     2341 atgaagcgga acgtaatgaa gcaatcgaaa agcaaaaagg cgaacattcg acaagtgtct
     2401 tttcaaaaaa tggtaataaa acaacaacca acttatatcc agtaacaaga acagacgttg
     2461 aacagcaaaa aaataatcaa taaacaatca aaccaacctt cagtagttag acaacattct
     2521 aatgcttgaa ggttggtatt ttttatgaaa aaaacatttt tttatttttt tcgtgtattt
     2581 ttccttttta
//
LOCUS       DANKHL010000262         2563 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000262 DANKHL010000000
VERSION     DANKHL010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.274"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..1087
                     /locus_tag="PW101_002706"
     CDS             <1..1087
                     /locus_tag="PW101_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2515075.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1294..2436)
                     /locus_tag="PW101_002707"
     CDS             complement(1294..2436)
                     /locus_tag="PW101_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2515076.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2441..>2563)
                     /locus_tag="PW101_002708"
     CDS             complement(2441..>2563)
                     /locus_tag="PW101_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HDL2515077.1"
                     /translation="IFKECKELLVEKGYFFYRNSKIKRIPISILEEVLHISWEE"
BASE COUNT          859 a          464 c          409 g          831 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa tccatgtaaa catggtttat
     1141 tttttacact gttttcaaac aaacccttgc tatttcttcg gttcgtcctc gtccattttc
     1201 gataccgaca gctagcttat aacccaaggt atatcaacat aaaaaaataa aaataggcta
     1261 tgacagcagc gtgacagcaa aaaagatgga aagttaaata tcaaacctcg taatttcatt
     1321 tgcgagtgct tgatttgttt ctggatataa gtgtccatat ttgtccaaag tagttttaat
     1381 agaggcatga cctagacgct tagatattgc atagatattt ttattatgtt caattaaaaa
     1441 ggcaacatgc gagtgtctta atccatgcat atgaattggt tttactcctg caatttcagc
     1501 tagttttttt attctgctta aaatagttct tggagatggc ggtaatccat caaaagaaaa
     1561 aaccaattct atatttttcc caattgattg ttgagttttt ttccatgttt ttaaatcttc
     1621 aacggtttgt ctgtctaacg aaattatccg aatacttgat gtattttttg tttcagcaaa
     1681 ttcataatct tgtcttgtac ggatgtaaat tgacttattt acgctacatc tgccatttat
     1741 taagtcaaag tctttccatt gtaacgctaa gagctcctca ctccttacac ctgtaacaaa
     1801 aataaaacgc atcaaacgtt tataaaattc ttcataaaaa tccccgtgat aaacactatt
     1861 atatactttc ttaaattcat caactgtcca aaattctact tgaggacgct gagtttttat
     1921 tttacctata gtatctacag gattttcttc aataatttca agtacaattg cccttttaaa
     1981 aactactctc aacttattga atatctgttt aatataattt ggtgataatg gttgctcaat
     2041 gccctttttt acaacatgga ttttcgataa aaactgttga aattcctgta tatgaattgg
     2101 tcgaatcttt acaacctgca tttctccaaa aaaatccaat gcacgttgta aaatatgatc
     2161 tgttttcata tatgtttttt ctacagttcc caacttatac caaggaaaaa aatactctcg
     2221 ataaaaatca tgaaacgtta ttccttgcag ataaattcct tgtgaatcag aaaaactttg
     2281 ttttaactct tcgagtcccc ttctagcttc tccttgagtt ttgaagcctc ttctggtcac
     2341 ttgaattctt tttccaaagc gatcttgtcc taaagttgca gaaaaaaacc aagtgcctct
     2401 ttttctgtct ttataaacat tcgcaatttt cctcatttta ctattcctcc caagaaatat
     2461 ggagaacttc ttctaaaatt gaaataggaa ttctttttat ttttgaatta cgataaaaaa
     2521 agtatccttt ctcaactaaa agttctttac attccttgaa gat
//
LOCUS       DANKHL010000263         2550 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000263 DANKHL010000000
VERSION     DANKHL010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.275"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            40..2481
                     /locus_tag="PW101_002709"
     CDS             40..2481
                     /locus_tag="PW101_002709"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDL2515078.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT          847 a          457 c          605 g          641 t
ORIGIN      
        1 acagcaaaga atggtatatt tttgtaggag gtggcttact tgaaagaagt cgttatgata
       61 gatgctgcaa gaacgccgat aggaaagtat cgcggaagtt taagtccatt tactgcagtt
      121 gaattaggca cacttgtgac taaaggatta ttagataaaa caaaactgaa aaaagataaa
      181 atcgatcaag taatatttgg aaatgtcctg caagctggta atggtcaaaa tgttgccaga
      241 cagattgctt tgaacagcgg tctccctgta gatgtcccag caatgacgat caatgaagtt
      301 tgcggatctg gtatgaaagc agtgatctta gcacgccagc tgatccaatt aggtgaagca
      361 gaacttgtga tagccggcgg aacagagagt atgtctcaag cacctatgct gaaacagtat
      421 cagtcagaaa caaatgaata tggtgaacca atttccagta tggtcaacga cggattgact
      481 gacgcatttt caaatgcaca tatgggatta accgcagaga atgttgcaac acaattttct
      541 gtgagcagag aagagcagga tcgctatgcc ttgtcgtccc agttgaaagc agcacatgct
      601 gtcgaagccg gtgtattttc tgaggagatc atcccagtca agatttctga tgaagacgtg
      661 ttatctgagg atgaagcagt tcgtggaaat agtacattgg aaaaactggg cacgttacgt
      721 acagtattct cagaagaagg aactgtaaca gcaggaaatg cttccccgtt gaatgacggt
      781 gcctctgtgg tgatccttgc atccaaagaa tacgcagaaa ataataatct gccttattta
      841 gcaaccatca aagaagtagc ggaggtcggt attgacccaa gtatcatggg aatcgctcca
      901 atcaaagcca ttcaaaaatt gacagataga tcaggaatga atttatcaac aatcgatctt
      961 tttgagatca atgaagcatt tgcagcttct tctatcgtag ttagccaaga actgcagtta
     1021 gatgaagaaa aggtcaatat atatggagga gcaatcgcat taggccatcc aattggtgca
     1081 agcggtgcac gtatcttgac tacattagct tacggattgc ttcgtgaaca gaagagatac
     1141 ggtattgctt cattatgtat tggtggaggt ttaggcttgg cagtcttgtt agaagcgaat
     1201 atggagcaaa cacataagga tgtgcaaaaa aaaaagtttt atcagcttac gccatctgaa
     1261 cggcgcagtc aattaataga aaaaaatgta ttgacacaag aaactgcgtt gatttttcag
     1321 gaacaaacac tttctgaaga gctctccgat cacatgatcg aaaaccaagt gagtgaagtg
     1381 gaaatcccaa tggggatcgc gcagaatttt caaatcaatg gcaagaaaaa atggattccg
     1441 atggcaacag aggaaccatc tgtcattgca gctgcaagta atggtgcaaa aatctgtgga
     1501 aatatctgcg cagaaacacc tcagcggctg atgcgagggc aaatcgttct atcaggaaaa
     1561 tcggagtatc aagcagtgat caatgcagtt aatcatcgaa aagaagagct gattctttgt
     1621 gcaaatgaaa gttatccttc aatcgtgaaa cgcgggggcg gtgttcaaga tatctctact
     1681 cgggaattca tggggagttt ccatgcatac ttatctattg attttttagt ggatgtaaaa
     1741 gatgcaatgg gcgctaacat gatcaactcg attcttgaga gtgtggcaaa caagttacgg
     1801 gagtggttcc ctgaagaaga gattctattc agtattttaa gtaattttgc tacagaatca
     1861 ttggcttctg cctgttgtga aatcccattc gaacgcttag ggagaaacaa agaaatcggt
     1921 gaacaaattg ccaaaaaaat ccaacaggca ggggaatatg cgaagcttga cccttaccgt
     1981 gcagcaacgc acaacaaagg gatcatgaat ggtatagaag ctgtagtggc agctacagga
     2041 aatgatacac gtgcggttag cgcttcgatc cacgcctacg cggcaagaaa tggattatac
     2101 caaggattaa cagactggca gatcaaaggg gataaactag ttggaaaact aacggtgcct
     2161 ttggctgttg ccacagtagg cggagcttcc aatatcctgc caaaagcaaa agcatcttta
     2221 gctatgttag atattgattc agccaaggaa ctagcacaag taattgctgc agtagggtta
     2281 gctcaaaacc tggctgcttt gcgtgcatta gttacagaag gaatccaaaa aggccacatg
     2341 ggacttcaag ctcgttcatt agcgatctca attggggcta ttggtgagga aatcgagcaa
     2401 gttgcgaaaa aattacgtga agcagaaaag atgaaccagc agacagctat acaaatattg
     2461 gaaaaaatcc gggaaaaata aaaaagcaat atctttagaa gaataaagca agatttacag
     2521 ttattttatt tactgcagat cttgcttttt
//
LOCUS       DANKHL010000264         2542 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000264 DANKHL010000000
VERSION     DANKHL010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.276"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(92..1141)
                     /gene="recA"
                     /locus_tag="PW101_002710"
     CDS             complement(92..1141)
                     /gene="recA"
                     /locus_tag="PW101_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDL2515079.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1250..2491)
                     /locus_tag="PW101_002711"
     CDS             complement(1250..2491)
                     /locus_tag="PW101_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDL2515080.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
BASE COUNT          657 a          582 c          454 g          849 t
ORIGIN      
        1 aataactcac gatttcttta ttcaataaaa aaacggcagg tcaaggagag tacctactct
       61 tcagaccagc cgtttttgtt tatcataatt tttattcttc tactaaaggc aattcttctt
      121 gtcctttttc atcttctggg acagcgactt cttcaccaat accataagct gctctaacta
      181 gagcagaaac ttctgccatc atttcaggat gattagccat atagatttta gcgttttccc
      241 gtccttgacc aatccgatct tctttataag aataccaagc accgctttta tcaacaatgt
      301 ctttttctac tgccatgtct agaagttctc cttcttgtga gatccctaaa ccatacatga
      361 catctacttc agcgattttg aaaggaggtg ctactttgtt ttttacgact ttgatctttg
      421 tacggttacc aacaatatct gtaccttgtt tcaattgttc tgcacgacgt acttccaaac
      481 ggatcgttgc gtagaatttc aatgcgcgtc ctccaggagt gatttctgga ttcccaaaca
      541 tcacgcctac tttttcacgg atttggttga tgaaaatagc gattgttttt gttttgttga
      601 tcgaaccaga gagtttacgc aatgcttgag acatcaaacg tgcttgtaac ccgacatgtg
      661 agtcacccat ttcgccgtcg atttctgctc gtggaactaa agcagcaact gaatcgacta
      721 ctacgatatc tacagcccca cttgatacta aagcatcagc gatctctaga ccttgttctc
      781 ctgtgtcagg ctgtgaaaga agtagttcat cgatattcac acctaatttt tgtgcatatt
      841 gcggatctaa cgcatgctca gcatcaatga aagcggccgt tccgccattt ttttgtactt
      901 ctgcaatagc gtgtagtgca actgttgttt tacctgaact ttcaggtcca tatacttcga
      961 tgatacgtcc acgggggtaa ccgccgacac ctaaagcgac atctaacgca agagagccac
     1021 ttggaattgt agagatttgt tggtcgattt tttcgcccaa tttcataata gagcctttac
     1081 catagctctt ttcaattttt ttcagtgcag catctaaggc tgctttacga tcatctgcca
     1141 atacgataat cctccttata tttacaaatc agagcaacat ttcattatcc ttatcatatc
     1201 ttttttagtg aaaaaaagca agaaaaaacc gaacaaatat tcgcatttat cattttctta
     1261 aaagctgttt tctcaaaaga tcaaatcctt gcataaccgc attatatctg atagcagcac
     1321 gatctctggc aaagtgatat ttttccgaaa ccacgccgtc tttagatgaa atagcgatcc
     1381 atactgttcc tgcagattga ccttctaacg tgtccggtcc agccactccg gtgaaagcta
     1441 cactgtaatc tgttcttgct atctcacgtg cttgaatggc catctgctcc acacattctt
     1501 tgctgactgt tccatattct gctaatagct ccggatcaat ctccagaaaa ccagcttttg
     1561 tttggagcga gtaggtaacg aatccgcctg gaaatacttc agatacacca gcaatgtcgc
     1621 ctaatgcggc ttggaaggcg ccggctgtca aactttccgc tgctgttaca gtctgcttgt
     1681 tttctttcag tagttccact acgacttttg ctagcgaatt atcatcgccg tatccataaa
     1741 aatattcccc tactcgttct tggatttttt cttctaatgc taaaagagct tgattccctg
     1801 cctgaacatc atttgttttc actgtcagtc gtaatgtcac ttcatttggt ttggcatatg
     1861 gtgcgatcgt tggattgatc tgtgtctcaa tcagatcttt tagctcagtg accagtcgcg
     1921 attcaccaat cccataaaag cgcaaaactt tagaaattag tttttcttca gaaggaaaac
     1981 gttcctctag taaaggacgt acttgttcca caaacattgg tttcaactca ttaggcggac
     2041 cgggaagaag gatatacgca tgtgtatctg tttgataaaa agtccctaaa gcaagccctg
     2101 tacgatttgg tagaggaacc ccgccttcga taatctggga ctgttgcaga ttgtttttgg
     2161 tcatttttcg atgggttgtt tcaaaatatg ccaataattt tttgtatcct tccgtatttt
     2221 gtatgagaga cttaccgagg tgagccgcag ttgcttcttt tgtcaggtca tcttctgtcg
     2281 gtcctaatcc gccacataag agtatgagtt cacttcttgt ttcagctaga gcaatcaact
     2341 cttctaaacg ctgctgatta tctcctacaa ctgtttgata ataaacttcg atacctagat
     2401 cagctagctg ttctgacaag aaagtggcat tcgtattcac gacttgtcct agtaaaagtt
     2461 ctgttccgac agctattatt tctgctttca ttacttcccg ctcccttata tagtatacgc
     2521 tgtattttta tattttttta tt
//
LOCUS       DANKHL010000265         2471 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000265 DANKHL010000000
VERSION     DANKHL010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.277"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            46..1191
                     /locus_tag="PW101_002712"
     CDS             46..1191
                     /locus_tag="PW101_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2515081.1"
                     /translation="MATIKQYEKKDGTKAWMFQTYLGISPLTGKEVRTTRRNFKTKKE
                     AQIELNRLLVDFEENGLKRETNETFQEVYDLWYESYKTTVKEVTQLKIEIKFNKWILP
                     KYGQFRIKEITTKHVQKILNQWAKTTDQYKVLHSTVSRIFKYAMTLGLITKNPCELVI
                     MPNRNVEKKKDTVKIYSRDQLETLFQYLESKRSTYRAEYDKTLLRFLFFSGCRISEAL
                     ALDWSDIDFDSNTVTINKTLSQTKYGYKISSPKTRNSYGSLSLDNKTINWLKKWQINQ
                     KRYMLTLGITDPTMIFCGIYKEIITKHAIYSRMITICKNSGIPFLGNHVTRHTHASML
                     LESGVSMKEVQERLRHSSIKLTMDTYAHLTKETKEKTVEKLAEHLNF"
     gene            complement(1276..1581)
                     /locus_tag="PW101_002713"
     CDS             complement(1276..1581)
                     /locus_tag="PW101_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDL2515082.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(1606..2037)
                     /gene="ruvX"
                     /locus_tag="PW101_002714"
     CDS             complement(1606..2037)
                     /gene="ruvX"
                     /locus_tag="PW101_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDL2515083.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(2034..2300)
                     /locus_tag="PW101_002715"
     CDS             complement(2034..2300)
                     /locus_tag="PW101_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDL2515084.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
BASE COUNT          810 a          511 c          400 g          750 t
ORIGIN      
        1 ttttcctatc aaaagcacgc cacgcaactg ataggaggaa aaacaatggc aacaattaaa
       61 caatatgaaa agaaagacgg aaccaaagct tggatgtttc aaacatacct tggaatcagc
      121 ccgctaactg gtaaagaagt ccgtactaca cgtagaaatt ttaaaacaaa aaaagaagct
      181 caaatagagc ttaatagact acttgtagac tttgaagaga atggacttaa aagggaaacg
      241 aacgaaactt tccaagaagt gtatgatctc tggtatgaaa gctataaaac tactgtaaaa
      301 gaagtcactc aattaaaaat tgagatcaag tttaataaat ggattcttcc aaaatatggg
      361 cagtttagaa ttaaagaaat tactacaaaa catgttcaga agatattgaa tcagtgggct
      421 aaaacaacag atcaatataa agtcctacat tctaccgtaa gccgtatttt caaatacgca
      481 atgactctag ggctgatcac taaaaatccc tgtgagctcg taatcatgcc caacagaaat
      541 gttgaaaaaa agaaggatac tgtcaaaatt tattcacgag atcaattaga aaccttattt
      601 caatatttgg aatccaaaag aagtacgtat agggcagagt acgataaaac tttactgcgc
      661 tttctatttt ttagtggctg tcgaatcagt gaggctttag ccttagactg gtcagatatt
      721 gactttgatt caaacaccgt cactataaac aaaaccttaa gccaaacaaa atatgggtac
      781 aaaatttcaa gtccaaaaac gagaaatagc tatggttccc tatctttaga caataaaacc
      841 attaattggc taaagaaatg gcaaatcaat caaaaaagat atatgttaac cctcgggatt
      901 actgatccaa caatgatttt ttgtggaatc tacaaagaaa tcattactaa acacgctatc
      961 tattctcgta tgatcacaat ctgcaagaat tctggtattc catttctagg taaccatgta
     1021 accagacaca ctcatgctag tatgctgtta gagtcgggag tctccatgaa ggaagttcaa
     1081 gaacgcctta gacactcatc aatcaaatta acaatggata cctatgctca tctaactaaa
     1141 gaaaccaaag aaaaaaccgt tgaaaagctt gcagagcacc taaatttcta gcggaacgta
     1201 ttcaatcccg tattcaattc aaaacaaaaa aaagctgaac ccttatctac caagggttca
     1261 gctatcattt acagcttatt cttcttcttc cgccatgaat gtattgaata cttcttcaat
     1321 catgtcccat tctgcgtctg tttcgatttg ttctaactcg ccttctgttc catcttcgtt
     1381 ttcgatgtat gcataagctt gtaattctac atcttcatct tctgaaacac cagcaggata
     1441 taaaagaaca tagttacgtc caaattcttc ttgtccgtca actgttagta ggatttcata
     1501 taatgtttca tttccttggt catccaccaa tgtgatgtgt tcgtgtcctt catggtcgtg
     1561 ggaatggtta tgttctgtca taagattacc tcttttcttt tattattaaa ataattgctt
     1621 acctgagccg tctaaataat tttgtaagat catcacagct gctaatttat cgatgacttt
     1681 ttttcgtttg gctcttgaag tatttgcctg ctctactagc atacgttctg cctgaacagt
     1741 ggttaatcgt tcatcttgat aatcaactgg caaggagaat agttctttga tcttttctcc
     1801 gtatgacatg gaagcttctg cccttggtcc gatggaattg ttcatatttt taggtaatcc
     1861 aactacaaac cggtctactt catattgtgc tactaattcc ttcaaccgat caaagccaaa
     1921 ctcttcttct gcttcattga tccgaatgat ctctactcct tgagctgtcc agccaaaggg
     1981 gtcactgata gcaacaccta ctgtcttaga accgacatcc aagcccatta ctctcataaa
     2041 ttcactccat gatttgagag gtagtccttg acaattactt ccatgatctc atcgcgttcg
     2101 tgacggcgaa tcaaatttcg agcatcacga taacgaggaa tgtaggccgg gtctccagaa
     2161 agcaagtagc cgacaatctg attgattgga ttgtagcctt tttcttccaa agcccgataa
     2221 acaatcgcta atgtttcgct cacttctttt ttgcgactat cgtcaaaacc aaaacgtacg
     2281 gtttcatctg taaaacccat gattctacac ctctttcccg caaggtaatt ccagcttacc
     2341 ttctacaacc ttattttact cgaagaaagg gaaaactaca aacaaatatt ccaaacttcc
     2401 gaataaatta agaaacacta tttgtatgtt tttttccttt tttctgtagc tttatcaaaa
     2461 gtactctttt c
//
LOCUS       DANKHL010000266         2427 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000266 DANKHL010000000
VERSION     DANKHL010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.278"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..1338)
                     /locus_tag="PW101_002716"
     CDS             complement(<1..1338)
                     /locus_tag="PW101_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2515085.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNG"
     gene            complement(1349..2017)
                     /locus_tag="PW101_002717"
     CDS             complement(1349..2017)
                     /locus_tag="PW101_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL2515086.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(2148..>2427)
                     /locus_tag="PW101_002718"
     CDS             complement(2148..>2427)
                     /locus_tag="PW101_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2515087.1"
                     /translation="LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFN
                     EEEIHFYIYSFYATFYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT          842 a          413 c          308 g          864 t
ORIGIN      
        1 accatttttt aaataaataa ttctattgca tgttttataa agaaattccg gatcatgagt
       61 ggctataact actgcaccct tttcttttaa gtctttaata aggtcagcaa tcctttccat
      121 attaatataa tccaacccac ttgtaggttc atcaaataga aataaattag tttttgacaa
      181 tgaagctata gaaaccaata atctttgttt ttctccaccg gataaatcaa acggtaattg
      241 atctttcatt ttataaagtt ccattttttt taaaattttt tcttgctgat ctttactaac
      301 tatttcattc cccagggcta actcgctcct cactgattct gtgaaaaatt ggtaatccat
      361 ttcttgcatt actaaaaacg gcgatgacaa atttttttta ttttcgatta tctttccttt
      421 ggtagggcga ataatcttca tgagtattct tagtaaggtt gttttcccca caccgttttt
      481 ccctaccaaa ccaataacat ctccaacttt gacatcaaaa tttatgtctt ttagaatatt
      541 ttcataagta atttcttcaa cttttaatac atctgcactt accttttcga tatagggaac
      601 ttcaatcgaa ctattaaaaa tgtcaaattt ccttaatgcg ttattgtttt cagtttttaa
      661 caactgaata gttgtagttt ttacagttct atttttaaca aatagaactt gatcaattaa
      721 ttctttcaaa tagtaaaaac gatgttcagc tataacaata gtaaatcctt tattttttaa
      781 atcacaaagt aagtcagcaa gtttcttagt cgctttgaag tccaaatttg aagacggttc
      841 atccaatata attaatttag agtttagcga taaactacat ccaatagaaa ttttctgccg
      901 ttcaccactt gatagcgaaa aaatgttttt atctaatata tttgaaagat ctaataattc
      961 agctaattca ttgattcttt tatccatatg ctcttttgta aatccaaagt tttccatcga
     1021 aaatactaac tcactcgttg tatttgttgt aaagaattgt cctctaggat cttgacttac
     1081 gtagccaata ttttttgata tactttcttg tgttaaggat tgtagcgagt tattttcaaa
     1141 taaaatttcc ccaaccaatt ttccgtcata aagtcttgga ataagtccat tcattaactt
     1201 taatagtgtg gatttcccac tcccagaaga accagtaatc aatgtaagac tacctttttt
     1261 tattgaaaaa ttaaggtcaa caagcgcttg ttccttaaca tccttatatg aaaaagaaac
     1321 attgttaaat tcaaacaatt ctttcacctc aaatcaaaat acttaagaaa aaaatagtaa
     1381 cggaaaacag gatgatatag tctaataagc tcatgtttgt tttatagtaa gaacttcttt
     1441 tcacaggaga ttctatcccc tttgttattg cattagcggt catttcttca gcaatcatag
     1501 ttgcacgtat aagaacggga actaacagaa attccaaata ttgcagaggc tgaaaaaaag
     1561 acaagcgaat gtttctcatt tttaaagatt ctttaatcat tcttatttca gaacgataag
     1621 tcggaaagaa tctgatcact actgtaatac ttaacattat ttttttcggt aaatgtattt
     1681 tttctaaaga ggataataat tcattgctag tatagacact aaccaacaac catgagatca
     1741 ttacgaccgg caacattcga aatacaatat ataaggatgt cccaaaaaat atagtaaaaa
     1801 actctatctt gatagcatca ataattttca ttactaaaaa cagaactgaa taaatcaaga
     1861 tgaaataaat acatgtcttt attttacgaa acatcaatac taaaactgta gcaataatga
     1921 aacaccccac ttgaaatata tttccctttc ctaaaaaaat aatgaccgga aaaaaaatat
     1981 ttaaagtaat caaggttaac ggattgattt ttgacactct ttcacctcca aaaaataaga
     2041 tcgcactgtt atcttaagct gataaaaatg caccgttatt cagataacag tgcgatctta
     2101 tcaaaaagca taactgagaa caattctcaa tgcaagcttt tttttaatta actcagtaat
     2161 tcaatccacc caggaacaga aaattgatac aatcttctgg ttaatagcat agtcttttct
     2221 tttgaatagc tatgcttcaa tatatcatag aatgtagcgt agaatgagta aatgtagaag
     2281 tggatttctt cttcattaaa gactaaattt tgattgatca ttccgatagt ctttaactta
     2341 tctagacaac agactgtctt ttttgtcaca aatgatgtaa ttttttcaac aatatcttga
     2401 tatttggttc cagctgattg gaataaa
//
LOCUS       DANKHL010000267         2408 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000267 DANKHL010000000
VERSION     DANKHL010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.279"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..1368)
                     /locus_tag="PW101_002719"
     CDS             complement(join(<1..795,795..1368))
                     /locus_tag="PW101_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
                     /protein_id="HDL2515088.1"
                     /translation="MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINA
                     YQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWMRQ
                     FREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERERIEELEKRVRSLQIENA
                     FLKELRKLRKQEAQQRRMKQSHESSQASEDRFKLVELLETLKFPKAIFMYWQKRLDRK
                     NPDQEIEAEMLKVREKHKDYGCLRMTNELRNRGFSINKKKVQRLIKKLGIKVTTYTRK
                     SRRYNSYRGQVGAVAKNRIHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLD
                     PFMDMFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYR
                     NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYY
                     NNERIK"
     gene            1611..2066
                     /gene="tnpA"
                     /locus_tag="PW101_002720"
     CDS             1611..2066
                     /gene="tnpA"
                     /locus_tag="PW101_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDL2515089.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2134..2391
                     /locus_tag="PW101_002721"
     CDS             2134..2391
                     /locus_tag="PW101_002721"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515090.1"
                     /translation="MSTPSRGIKNTPVLTGVWVTDRLDFNWCQKLMSRKGDEILAIYG
                     YSRISTSNQDYKTQIQTLENAGAEIIFLENHWIIEHLWKYF"
BASE COUNT          715 a          454 c          460 g          779 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggatccg atttttggct actgctccga cttgtcctcg ataagaattg tagcgccgtg
      541 atttacgtgt gtatgtcgtt acttttattc ctagcttctt gatcaggcgc tgaactttct
      601 ttttattgat agagaaaccg cggtttctta gttcattggt catccgtaaa cagccataat
      661 ccttgtgctt ctcccgaact ttaagcattt ccgcttcaat ctcttgatct ggatttttac
      721 ggtccaatcg tttttgccag tacataaaga ttgccttagg gaactttaaa gtttctagaa
      781 gctcaactaa tttaaacggt cctcggaggc ttgcgatgat tcgtgcgatt gcttcattcg
      841 tcgttgttgg gcttcctgtt ttcgcagctt cctcaattct tttaaaaagg cattttcaat
      901 ttgaagggaa cggactcgtt tctccaactc ctcgatgcgt tcacgttctt tagacgttgc
      961 tttagttgta gattttttct tatttttggg ttcatttttc ttagacaaga taggtggaca
     1021 tcctttcgtt tttgagagtc catcgatccc accctcacgg aattgacgca tccaactagc
     1081 gatcaaatta gggttgttca tgttaagtgt gttggcaact tcacgatagg acagctcact
     1141 tgttagatat aactctatcg catccagctt aaattgaaca gaatattttt tcttttgtcg
     1201 ctttcttact agaccctctt caccaaattc ttgataggca ttgatccagc gcttcacttg
     1261 tgatggagcc ttaatggaat atttctttgc cagataatca cttccgcctt taccatccaa
     1321 gtaatcatga acgatcttta atttaagttc aaaagtatac ttagccataa aaaataccga
     1381 cctccaaaag ttagattttt tggtctaact tttgggggtc ggtacaaaac ttaaaagttt
     1441 tagacccttt tattttctta atgacttcag tcagttttgt gcgtaaagcg tgcgaaacaa
     1501 gaaaaccgcg cgtttctacg cgtggaagaa gtatctttag cgataaaaac tcctttcgat
     1561 atactagtga gtggctacca accgctcatc agatcgaaag gagttttttc atgtcaaatg
     1621 acgataaaag tttagcatat actcgttgga attgtaagta tcatattatt tttacaccaa
     1681 aatatcaacg aaaagtaatc tatgggcaat tacgaaaaga tcttggaaaa atacttagga
     1741 agttgtgcga aatgaaagat gtggaaatca tagaagcaca tgcaatgcta gatcatattc
     1801 acatgcttgt tcgagtacca ccaaaaatat gtatatccag ttttatgggg tatctgaaag
     1861 gtcgtagtgc ggtactaatc cacgagcagc acgcaaatct caaatataaa tacggaaaca
     1921 aatccttttg gtcgaagaga tactatgtga gtacagtcgg attgaatcaa aagacaaatg
     1981 aaaaatacat ccgtgaacaa gaagctgatg atcgcattcg atatattata agtaaaagag
     2041 agtacgtaga accatttaaa aaataaagag aagtaagtga gtgaagatga gcagttggcg
     2101 gtcttttaaa aatccccttt taaggggcag cgagtgtcga ccccttctag ggggattaaa
     2161 aatacacccg tcctcacggg tgtatgggtt actgatagac ttgattttaa ctggtgtcag
     2221 aaacttatgt ccagaaaggg tgatgaaatt ttggcaattt acggctattc aagaattagc
     2281 acaagcaacc aagattacaa aacacaaatt caaacattag aaaatgctgg cgcagaaata
     2341 atcttcctag aaaatcactg gattatcgaa cacctttgga agtatttttg agttacgtaa
     2401 gtattgat
//
LOCUS       DANKHL010000268         2400 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000268 DANKHL010000000
VERSION     DANKHL010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.280"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            207..2069
                     /locus_tag="PW101_002722"
     CDS             207..2069
                     /locus_tag="PW101_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2515091.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="PW101_002723"
     CDS             2069..>2400
                     /locus_tag="PW101_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2515092.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DANKHL010000269         2379 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000269 DANKHL010000000
VERSION     DANKHL010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.281"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(8..253)
                     /locus_tag="PW101_002724"
     CDS             complement(8..253)
                     /locus_tag="PW101_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515093.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(270..1121)
                     /locus_tag="PW101_002725"
     CDS             complement(270..1121)
                     /locus_tag="PW101_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2515094.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1287..2291
                     /locus_tag="PW101_002726"
     CDS             1287..2291
                     /locus_tag="PW101_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDL2515095.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT          757 a          403 c          478 g          741 t
ORIGIN      
        1 attctttcta ttttttcggt atcaaggcaa aaacaggtag ttcaggtgaa taaatgactt
       61 catgttcgtg ccagggtttg accattagcg aatgatcgaa ccgttttttc agttcttcga
      121 tgatttgatc atgtgtccag tttcttgcag gaaaatgttg gattttctct gcatcgatca
      181 aaaagacaaa atcggaatga aaagtctggg taacttcttc cgagagagaa ttcaagcgtt
      241 cgattggatc cataaaatca cctttcgact taaaattggg tagtatctgg gtcttttgct
      301 ttgccgacta caccatctaa ctgatcgatt tgtttcattt cttcttctgt taattcaaaa
      361 tcaaataatt cagtattttc tttgattctg cttggtgtca ctgacttagg aagaggaagg
      421 aattcgtgtt gcagcgacca acgcaaagca acttgtgcaa tcgtcttgtc atgtgcttca
      481 gcgatttgtt tcatttcagg cacatcgaag attttaccag tgcctaatgg actatatgct
      541 tcgaggatca tatcatgttt ttttgcatac tcaactacag ttgtctgtaa ttcgcctggt
      601 gcaaggaaaa cttgattgac catcggcata atttttgctg tttttgctag tgtatctaga
      661 tggtggggta agaaattgct cacaccgatt gctttgattt tacctgcttc atacagttct
      721 tcaaatgctc gccatgtatc tgcatttgct tgttcccaat tatcacgaaa agcaacaggg
      781 tttggccagt ggatcaaaaa cagatctaaa taatctgttt ggagcttctt caatgattct
      841 tcaaaagaag acatcacaag ttcatagctg tggtttgcat tccataattt tgttgtcaag
      901 aatatctctt ctctaggaat tccactttcc ttaatcgctt ggccgacgct ttcttcattc
      961 ttgtatcctt gggcagtatc gatatggcga taacctgcag ccagcgcttc tttaacagag
     1021 gaaacagcga catccccatc aggtgtttgc catgtgccga atcctactac cgggatttcg
     1081 taaccatttg atagacgata agtgcttgtt aatgaattca tactttcagc tcctttttta
     1141 ttcgttcaac tataagtgta gcgcattttt cgaaaaaaag aatccatttt gtttctactc
     1201 ttgaaatttt tgtttaaaag aaaatgattt tgattctggt atttttgatt ttttttccgt
     1261 aaactaagac agaagggaat gagaaaatgg atatctattt aaaaaaagca attctgcata
     1321 ttattgacag agaaactggt tctccagtct tttcacaaaa agagctggat ctgacaaaag
     1381 aatatattcg tgattttctt caaaagaaga tccaaaaaat ctcttctgct caaacaaaaa
     1441 ctggacaact agcagaagaa agcatgatcg ttgaatcact taaaatcgct caagaaaatt
     1501 ttgtggaagc aagtgaagca atcgttcagc ggtggttcga tatctaccaa gaaagtgaag
     1561 aagcacctag tgcagatgta tttgttgtct tgtatgaatt agatacagtg atgtacttat
     1621 ctttattgaa agtcaattat cgtgaggctt acactcactt tgttgaagca gacgaagcag
     1681 ggatcgacaa ccgcttgatt ttgaatcgag caattctagg ggcgaaaaca caaaaagcag
     1741 atgaagcatt cgctgtgaat ttgactgatt ggacctacga attaattgaa aaaaaatatg
     1801 agttttctgg caaaaaagag ctttattttt ctggaagagt gatcgaaagt gttcctgcac
     1861 cttctttaga agaaaatgta aaagtgatcc aaaaagtagc caagcatctt ggaaaaaaat
     1921 ttgagacaga agaatttgat atcatggctg atttgaaaga agctgtctat gatacgatag
     1981 aagaaaaagg tcggttggat catgagatga ttgcggaaaa agtcttcaag gaaaacatca
     2041 cggcaaaaat ggcttttaaa gaagaagtac aagagcaagg atttgtccca gaagcacctc
     2101 ctgtaaaaga agtacaagaa atttctgaga aaaaatttgg aaaacaaaaa ttcaagctat
     2161 caaacgggat cgaactgatc gtaccagtag atgtatatcg caacccagat ttgattgaat
     2221 ttgtgaataa tccggatggt acgatatccg tgatgatcaa aaatgtggat gaagtattga
     2281 atcgtttata aaaatgacta gaatattagg tgttcaagta ccagtgatca gttaaatgaa
     2341 ttgtgtcaag agaaggatcg gaaaagacga atataacaa
//
LOCUS       DANKHL010000270         2364 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000270 DANKHL010000000
VERSION     DANKHL010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.282"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            184..2328
                     /locus_tag="PW101_002727"
     CDS             184..2328
                     /locus_tag="PW101_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2515096.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
BASE COUNT          829 a          492 c          454 g          589 t
ORIGIN      
        1 taaaatgaac gtcaaagaaa aacgaatgaa tgctcgttgc ttatttacaa attgttttat
       61 ttagaataca cagacagatt tatgaaaaga aaaagctttt cgacacgatt atctgttcca
      121 aaaaatgtct aatgaatgct gaataagaca gaaaatcaat gaaaggaaaa ggactagttt
      181 attatgaaaa ataaaaaaac gatacgtttg ctagctgcta cttggctgat tgctccgtct
      241 gttttgtcga ccaataccgt atttgccgta gataatcaaa tagctgatca aacagtcaca
      301 acagatacga ctacatccag ctcagctact agtgaaacaa cggattcttt tacagaagac
      361 ccaactggaa caactaactc aacaacagga tcgaccgagc caactgaaat tgatccggct
      421 gatccatcag aaaatgaaaa gccagccgag acatattccc ttacaattgc tccttctttg
      481 attggtaaga tccaactgac tgcttcgaca gcggattccc ctgaacagca acctttggat
      541 attacggaaa gtggaacggt agaaggttta ttagcggaaa cgaaggtaac ttacactatt
      601 acgccagctg aaggtgaaca aattgcttca gtcgctgtta atggtgccaa agaagtaaat
      661 tatgatgcaa agacctttac agtaggtacg aacaatttat ctattactgc agaatttaaa
      721 gctggaccgg atacttcaac agagaaacca actgatccta gtgaagaagt ccctagtaca
      781 ccagatccgg aaacgccaaa caaaccagat gtgggtaata acaacaataa taatggttca
      841 aataattcaa acaataataa caataattcg aacagcggga attcttcgaa tcaaacaaac
      901 ccatctggat cgaataacaa tggaaatact cagagagaac caaaccatcc aaacaatggt
      961 tcatcgaatg gcggaacgaa caatgcttca tctcgtccaa atacagcaaa tcaaagcggt
     1021 atagagaatc catcttctgc ttcttctgat tttgttgtaa aatcaccaat tgatgctgta
     1081 ttgcctacaa atacaacgaa tgtccaacaa gcaatcgttc gagaagcatt caagcatctt
     1141 ggaaaaccat atgtatgggg agcaaaaggt ccaaatactt ttgactgttc tggtttgact
     1201 tattacgtat atatgaaagc aaccggtcat tatatcggtg gctggacagg cgaacagcaa
     1261 tatgcaggta cgcagattcc agtaagtcag gcccaacctg gcgacctcgt cttctgggga
     1321 ccttcttccg gtgttactca ccatgtaggt atctatattg ggaacggaca atttatccat
     1381 gcgcctcaac caggagataa agttcgagta acttctattt cagatttcac gcctgatttt
     1441 gctgtccgag taaacttagc aggtttacct gctgcttcag gttcgctgac aggcggctca
     1501 tctattttag atggattaga tggctcgttc catttcagtc aaaaccaaac aactgatcag
     1561 ttcttgaaaa agattgcaga tgatgcacag gaaatcgggc aaaaagaagg aatctacgca
     1621 tcagtcatga tggctcaagc tatccttgaa agtggatctg gaaacagctt attatcaagc
     1681 gaaccaaatc ataacctttt tggtatcaaa ggatcatata aaggaagcag cgtgaccttc
     1741 aatacacttg aacaagatag ttccggacaa agctaccaaa tccgcgcaca attccgtaaa
     1801 tatccttctt acaaggaaag tttggaagac tatgccgact tgatcaaaaa tggactgact
     1861 ggcaatcctg atttctacaa accaacttgg aagtcggaaa caaaagacta caaagaagca
     1921 accaaatatt tggaaggacg ttatgctaca gatcgccaat attcacaaaa attgaacgcg
     1981 atcatcgaag cgtacgacct gacaaaatat gatgaaccga aaaaagaagc agcaacaact
     2041 gaagaagcaa acgctgaaat cctcaatatg tctaagcgat tcgatgtgcc aatcaaatgg
     2101 acaacagatg gcgtgccaat cacaggatta gaattttcaa cgataaaacg cgtcaacaac
     2161 gcttcttcca tcttccgtgt cgcttcaatc tgggatttgt ggaacaattt tacggcaaga
     2221 cagataccag aaacaactac tcgtacagtt aaagctgcaa ctgttcctgt actaaccttg
     2281 ttaagtttag acaaatcatt ctggcttgat ctgcctttaa agaaatagaa taaattaaaa
     2341 aggggatgtg acactgtcac actc
//
LOCUS       DANKHL010000271         2353 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000271 DANKHL010000000
VERSION     DANKHL010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.283"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="PW101_002728"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="PW101_002728"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL2515097.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="PW101_002729"
     CDS             1011..2258
                     /locus_tag="PW101_002729"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDL2515098.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
BASE COUNT          762 a          449 c          501 g          641 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgacta caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgac cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gat
//
LOCUS       DANKHL010000272         2340 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000272 DANKHL010000000
VERSION     DANKHL010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.284"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(365..1657)
                     /locus_tag="PW101_002730"
     CDS             complement(365..1657)
                     /locus_tag="PW101_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL2515099.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1931..2191)
                     /locus_tag="PW101_002731"
     CDS             complement(1931..2191)
                     /locus_tag="PW101_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515100.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          781 a          441 c          355 g          763 t
ORIGIN      
        1 tatgggctac tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt
       61 ttgaaatcgt tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga
      121 tagaataaga tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa
      181 aaaatacgaa aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca
      241 ttcatttaaa ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact
      301 atgaaaaccg taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt
      361 gaaatcagat aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg
      421 taaatcgcat acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca
      481 ccatagccca gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta
      541 aataaagtag ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc
      601 tgaagctgga gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac
      661 taccataatc aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac
      721 cagaataatg agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag
      781 aagaagcacc gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa
      841 tatagcgcaa tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa
      901 agatcgcttt tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat
      961 cttcgacacc acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg
     1021 tttgatttaa ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg
     1081 tgtatacagc atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa
     1141 cgaatagaaa ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa
     1201 ggacagcgtc agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt
     1261 cgacggttgc ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc
     1321 cttcgatgtc ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt
     1381 cccggtctct aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc
     1441 catagccatt tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta
     1501 aatgattatt caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca
     1561 aagcatcttg catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta
     1621 acttttcggc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
     1681 ttgtagaaaa agaaaaaccg cgaagcaacc acctgcctag gattaatggt tacttacacg
     1741 gtttatatta cactctcatc attaaaaaag tatctttcga tattactaaa caatgaaatt
     1801 atctatttga aatttaaatc tgataatata ttgagtgttc caatgagggc tttgatccta
     1861 ttatccatat ctttttcagt tttaatattg atacacatta taagaaaaag aaacttttgg
     1921 ttaatttatt tcatcccagt gcaaaatata aaaaagtaaa atagaccata ggcaatagat
     1981 taacaattaa tataatacaa gcaaatattc tattcattgt atttctgctt tcaaaaagtt
     2041 ctaatattga aagtataatt ccacaaatgc ctataataag tatgggaata agattagaaa
     2101 ataacacccc gagccaatct gtgaaaatta ctatatttac taatatccct attaacatac
     2161 ctatcatatt tattaaatca atttttttca ttagatattt ctcctttatt aaatctgaag
     2221 tacttatatt aaaataattc taaactattt acctcaaaaa gtgcaataaa ttatttctga
     2281 acaataaaat gatagtcaaa acaaaaaaat attgttaatc gttcagagcc atccgagagt
//
LOCUS       DANKHL010000273         2308 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000273 DANKHL010000000
VERSION     DANKHL010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.285"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..642
                     /locus_tag="PW101_002732"
     CDS             <1..642
                     /locus_tag="PW101_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HDL2515101.1"
                     /translation="AESFEKKHQHVLRDIDALKKDVSNFGQMFVEGNEPDSYGRNRRV
                     FFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIRKNTVPQTIEDMMIYQLEEM
                     KDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGKESRAYEEISKKVFSKFWSE
                     FKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEIDQLNRQQRLFGDDNE"
     gene            820..1161
                     /locus_tag="PW101_002733"
     CDS             820..1161
                     /locus_tag="PW101_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515102.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            1154..1825
                     /locus_tag="PW101_002734"
     CDS             1154..1825
                     /locus_tag="PW101_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDL2515103.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            1831..>2308
                     /locus_tag="PW101_002735"
     CDS             1831..>2308
                     /locus_tag="PW101_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515104.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGK"
BASE COUNT          843 a          365 c          479 g          621 t
ORIGIN      
        1 gcagaaagct tcgagaaaaa acatcaacat gttttaagag atattgatgc actaaaaaaa
       61 gatgtgtcca attttggaca gatgtttgta gaaggtaatg aaccagattc atatggcaga
      121 aatcgacgag ttttcttcat tagtagagat ggatttttct tgctggctat ggggtttaca
      181 ggaaagaaag ctatctactt caaacaaaaa tacattgaag cattcaacga aatggaagat
      241 gttattcgca agaatactgt tcctcaaaca attgaagata tgatgatcta tcaattagaa
      301 gaaatgaaag atgttaaaaa agatgtttcc atgcttaaag atactatgcg aattagcgga
      361 caacaagagt ttgaaattaa gcaaaaagga aatatgaaag ttatgggagt tctaggggga
      421 aaagaaagcc gagcttatga agaaatcagc aaaaaagtat tctcaaaatt ttggtctgaa
      481 tttaaacgta ccttttcaat cccaagatat ggcgagttac ctcgtaagag attcgatgat
      541 gctgtttcat ttattgaaat gtggttgcca gaaactgcga tccgtatgga aattgatcaa
      601 ctgaacagac aacagagact ttttggtgat gacaatgaat agagctgaag cgctaagaat
      661 agggacggca attgctaatt gctggtggaa atactataaa ccaatcatcc taagccaaca
      721 acatattgac aagcaaaaag catggcaaca aataaaaaag tgactccgcc ggcaagcaaa
      781 gagtcacaaa gaaaacacat cataaggaga ttttagcata tggaaaaaga actttccact
      841 ctagatcaat atttgattga tcctgattgg ggcaagccga aaattgagga aacaagtggt
      901 cgaaaaatca gacgaaatct tttgacgaat gaagaactag cttgggatca agatgattta
      961 ggcaaccatg taactatttg ggatcatgtt tatcttatcc atctatcgaa gcattcgaat
     1021 aaacctgaat atatttacgt catcgaagat ggcttgattg atgcgctaga agagtacgac
     1081 agagataact tgattgatat ctcttattac ggaccaggta agaaatacat tgctgaaatg
     1141 gaggcagaat ttgatgagtg aaatcaaagg gacaacgaac tttgaaaaac tttttagtcg
     1201 taagttaaat aaaattctca agaaaaaagg aaattttgat tatttatctt gggctcacgc
     1261 gtgggagatt atgaaaaaga atgatccaca ggcaacggta actattaatg agtacaaaca
     1321 ctacagggtt gtttctggaa ctcatcaaga ctttcttgtt gaggaatata aaccttttct
     1381 tatggatgaa actgggactt atgtatctgt ctcagtaacg gttaaaggac acacggaaac
     1441 cgaattattt cctgttttag attatcgaaa ccaaccagtt gttaaaccaa atgcaatgca
     1501 aatcaataac tcattgaagc gatgctttgt gaaagcattg gctctacacg gactgggatt
     1561 atatgtattt caaggggaag atattccaac accacctaga atcgatacaa agaaattaaa
     1621 catgctagag acgattctag aagctttcaa tgagcagatg ggtaaagata tgaccaaaac
     1681 cttaatcgaa tatgttaatg agcagacaga taaattaggg ctcttagctg ataacgttga
     1741 aactattgaa cagttaagct atgagcaatg tgccttgatg gagcgagcaa tagcagctaa
     1801 gagaaaagaa ttagataaga agtgatatga gtgtttaaac cattaatcga ttcatattca
     1861 gcggttctga aaaagttcaa aggaaaagac ataagcgcaa ccatcaatga ggaagtgaac
     1921 attgatcgac taaagacgat gtatgacggc tacgatggtg atcgagtcat tgaaattcgt
     1981 tttattgatc ctagacgttt caccgtacag caacgaaact tcatctatgc gctgataggc
     2041 gatattttta tcgatacagg catgccaacg gacttctgga aggaattctt ctactttcgt
     2101 tttgaaggtg tcacagggcg caaaataagc ctgaaagacg aatcgaatac aactgtgagt
     2161 gatgccaatg tcttagcaaa tatcatctta gatttcatct ttgaacatca tattcctttc
     2221 aaagaaggct atgagatttt acctgcgaat caagaatatt acttctacaa atgcatcaca
     2281 aaaagagtct gctgcatctg tggcaaaa
//
LOCUS       DANKHL010000274         2293 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000274 DANKHL010000000
VERSION     DANKHL010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.286"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(51..1598)
                     /locus_tag="PW101_002736"
     CDS             complement(51..1598)
                     /locus_tag="PW101_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDL2515105.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="PW101_002737"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="PW101_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDL2515106.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="PW101_002738"
     CDS             complement(2043..2237)
                     /locus_tag="PW101_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515107.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          644 a          477 c          408 g          764 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agt
//
LOCUS       DANKHL010000275         2271 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000275 DANKHL010000000
VERSION     DANKHL010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.287"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..570
                     /locus_tag="PW101_002739"
     CDS             <1..570
                     /locus_tag="PW101_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HDL2515108.1"
                     /translation="RFIKKTNKKTPMILLIIGIIFTTVGFSLSSSNEANDNNKQESSS
                     SDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSYYSITPATEQNTAFTKYISGKTY
                     TFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKVSYVIFAKDLNNADTFTKGDKVT
                     FTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            683..1318
                     /locus_tag="PW101_002740"
     CDS             683..1318
                     /locus_tag="PW101_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515109.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            1617..>2271
                     /locus_tag="PW101_002741"
     CDS             1617..>2271
                     /locus_tag="PW101_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HDL2515110.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     "
BASE COUNT          890 a          354 c          391 g          636 t
ORIGIN      
        1 agatttatca aaaaaacaaa taagaaaaca cctatgattc tccttattat cgggattatt
       61 tttactaccg taggtttctc gctttcctca tcaaacgagg caaatgacaa taacaaacag
      121 gaaagctcat catctgatac tcaaactact acatctaaca gcagcaataa aagcctaact
      181 caatttgaag aatttttaga gacaaataat catgactggg gtgctttctt agatagttat
      241 tattctatta ctccagctac tgagcaaaat acagctttta ctaaatatat tagtggtaaa
      301 acatatactt ttgaaggaac tgttatcgaa tcaatgacta ctagaatagc cattatagca
      361 gacaaagagt acgatgataa aagttggaat gatatttctg caacacctaa agtttcatat
      421 gtaatttttg cgaaggactt aaacaatgct gataccttta caaagggcga taaagtaact
      481 tttacaggag aaattagttc aagaggatcg aacatagaaa aatcctatgc tcaatgggat
      541 atgattaata gtaaagtaag taaaaaataa atgaaccacg ccccactaag agttagattg
      601 tgcacaataa taaaacctat acaaaagagt gccatcagga gtcacaatgc ttgtaaagga
      661 aatgacaaaa taggaggtaa ctatggataa tagcaatgaa aaactaacca aaaaagaata
      721 tcttgaatta atagttgaag gcggtattgg agcaattcct tatattggtg gcgttgtgca
      781 gtcactttat tttgggtcta aaaacgagaa gcgtttcaaa cgcatcgaga aattctacaa
      841 aaatttaaac gaatcattag aaaacattcg cgatcaaatt ccagaagatg ttttcaactt
      901 agagaacaag gaccaactca ttggaatatt cgaagggata aatgacgagg tagaaaaatc
      961 aaaggcacaa aataaaatag atatgtataa aaatctttat aagaattgtt tacttcgaat
     1021 taacaacgct tcatgggata acgaggagta ttatcttctt gtactgaatc aattaacatc
     1081 gattgagatt caattgttag catgcttaat gagtcgtgga aacgaaaatt tcactggaaa
     1141 tatcagcatg tctggctatt cacaagagct aatagatggt agcttaaata gattatctga
     1201 ttttggtctg ttagaaaaaa cgattagctc catagtatta ggtgatgtcg gcaaacaaaa
     1261 tatgggctac aaaatatctc atttaggaat tcaatttatt aactcaactc ttacttaagt
     1321 tttctttcta atatgacatc tagacctatc agcaatgcaa caataaaaaa ccagacatac
     1381 caataatcaa caaataatct aaactctgga agttcagtta ttcttttaaa aatcacttta
     1441 atcataataa acacctctga ttggatgatc taatacacat ccaattatac cattaaaacg
     1501 acaaatagca catccttgcg ccggcaagca aaaaggatgt gcaaaaaata ataaaaccca
     1561 aacaatgggc ttctttatag ttcctattgt atcagagaaa gagagctgat tcaattatgg
     1621 caagattagt caaacgtgga aatagttggc aatacgaaat ttcatacaaa aaagatgacg
     1681 gaaaatacac gaagataaga aaatcaggat ttaagacaaa aggagaagca aaagatgccg
     1741 ccaacgaatt agaatataac ttgaacaaag gccttaaagg ggatcgcaaa aatctattat
     1801 tatcagatta ctttgaggat tggatgcaac tttataaaga aggaacagta tctcctatca
     1861 cttatagaaa atacgaagat acgttaatga acataaagaa atatatgcca gcggtattga
     1921 tttctgattt agatagagtg ggatatcaac gctttttaaa taagtatgct aaagaccatg
     1981 taaaatctac cgttattaag tttaataacc atattagagc gtcgttgaaa gatgccgtag
     2041 aagaaggatt aattccgttt gatccaacta gaaaagcagt aatcaaagga aaagattcat
     2101 tgaagccaaa agaagataaa tatttagatt atgatcaatt taaatcttta atgaaactcg
     2161 tagaagaaaa cctttctgcg cagtactctt ctcctatgct cgtgttagtt gctggtgcca
     2221 ctggaatgcg atttgctgaa cttctaggat taacatggga agatatcgat t
//
LOCUS       DANKHL010000276         2260 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000276 DANKHL010000000
VERSION     DANKHL010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.288"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            94..1707
                     /locus_tag="PW101_002742"
     CDS             94..1707
                     /locus_tag="PW101_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2515111.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          709 a          402 c          445 g          704 t
ORIGIN      
        1 gtctattcaa aataaacaat aattttccgg cacaattatt tgctgttttt taccataaca
       61 tagtacgatc aacgtagaaa aggagatgtt tttatgaaag ttgttgtcat tggcggtgta
      121 gctggcggtc cttcatttgc cactcgtttc cgtcgattga atgaagcgca cgaaatcatt
      181 atctatgaac gcggagaaaa tatttcttat gcaagttgtg ctttgcctta ttatttagga
      241 ggtgtgatca ctgaccgtga ctcgctgatc gaacgtacac cagaaatatt gaaaacaaaa
      301 aacaacatcg atgtctttac taaacacgaa gtaacagcaa tcgatccttc tactaagcga
      361 ttaacagtta aagacctatc cacaaatgaa gaaataaaaa cagattacga taagttgatc
      421 atctcttctg gtgctagacc agattatccg gatattcccg gagtttttga agcagaaaac
      481 ggctttgtcc tccgtagtgt gacggatgcg gatcgaatca aatcgttcct tgaagaaaaa
      541 aatccacaac atgtcgtcat tcttggtgca ggtgttatgg gtctggaatt agctgagaat
      601 ttcaagcatc gcggcttaga cgtgacttta atcgatcaat tgccacaagt cgctttccct
      661 tatgatccag aaattgctaa tttagtttat gacaaattgc tgaaagaagg attagccgtt
      721 catttagaaa caagagttac tgagatccgt gataaaggtc gagaaatagt attatcagat
      781 ggctccgtcc tttctgctga tatgctaatt tttgctgttg gtgtttctcc gaagaatgaa
      841 gtggtgaaag caaccggcat acaattatca gatacaggtc agatcattgt tgatgaccag
      901 ttacaaacca atctgccgga catctatgcg attggcgata ttatcgaaac gactagtgta
      961 gtgactggtc agccgatcca aagtatgctt tccagtgcgg ccaatcgtca aggacatatg
     1021 ctggcagata ttttaaatgg tacgcctatg cgctatcgcg gatatattgg tgcaggtgtc
     1081 gcaaaaatct ttgatcatac agcaagttat gcgggaatga cagaacatga attaaaagca
     1141 tcaggcataa caaattataa aactgttttt atcactccct ttgaccatgc ctatttctat
     1201 ccagaagcta caagattaaa tctaaagctg atttttgatg cagatagcgg tcgtatttta
     1261 ggtggacaag catttggaga aaaaggtgtc gataaacgga tgggagaact ttctgtagca
     1321 atcaccggaa acttgacagt ctttgatttg cctgatttgg agttgcctta ctctccaccg
     1381 tattctacta cccgtgaccc gttgaatata gctggttatg tcgcaatcaa tcaaatgacg
     1441 aatatcgtag aaacgatcaa agcaagtgat atacccgaaa acgatttgaa agaagcgttc
     1501 tttttagata tacgtgaacc taataaagca ccttctggca gtatctcggc aacaaaaaat
     1561 attccaatga atgagttacg agatcgaatc aatgaaatcc caaaagataa aaaagtttac
     1621 attactttca gaagaggatt gaatacttat acttctgccc gaatcttggc aggtttgggt
     1681 atcaaagcga ttttgattga agaataaaaa aacgaccgcg gtgggtagcc gcggtctagg
     1741 agttattttt acttgaggag taaaaatgaa aaagtatata gggttgttgg ttggtatgta
     1801 tatagaatag aagataaatg tgaagaaatt gtgcattatt tcttcacatt tattaggtta
     1861 tagtttaaga aagtttaaac attgcgcaat ttaattattt tagctgtttc ttttcataaa
     1921 aaaacatgtt gtgactctga gacaaatcat agtccatagt gatgtctcta gagtaactac
     1981 gcctaatatt acgaggcatc atattaatct atcagcacta gtacctaaac tagcatccct
     2041 ttcgaatatt ttttcttata taataaagtt tgctttcact cctctaatct caccaaatcg
     2101 actggcttgc gttgtgtgat actaggaact gattggttta gaacgacttt tctattgtaa
     2161 tcatattttt cgccatcctc gttgtcttct aatccttcaa tgaatgatcc ctaatgtgtg
     2221 tattaaataa tttaattttt ttgttcctat ttatttaaca
//
LOCUS       DANKHL010000277         2213 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000277 DANKHL010000000
VERSION     DANKHL010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.289"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            378..615
                     /locus_tag="PW101_002743"
                     /pseudo
     CDS             378..615
                     /locus_tag="PW101_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(759..1535)
                     /locus_tag="PW101_002744"
     CDS             complement(759..1535)
                     /locus_tag="PW101_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2515112.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            1658..2065
                     /locus_tag="PW101_002745"
     CDS             1658..2065
                     /locus_tag="PW101_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDL2515113.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGC
                     YLLECRFTSKKSMNSFLEQLNEYANYKLSIVID"
BASE COUNT          752 a          359 c          338 g          764 t
ORIGIN      
        1 caaacggaaa aatagttcct catattttgc cgtttgttga gctaataccg aaggtcagct
       61 gattgaacac cgtttaagaa agaggtcgtg agcctgtcat ttaactctga gtcacaagta
      121 acaatttcaa aaaacagctt ctcatctttg atgaatatcg tgacttgtgg acgaggcgtt
      181 gataagtgaa tcatcttttt gttacctatt ttttctgtaa ccaaaaaacg atggaacgtt
      241 gttttgattg cccttaatga ggacagtcgg aacaggtttg tcatttttat atttaaatgg
      301 cacaaatttt tctaattatt tgtcatttag cgctgaaaat ttttgctatt tatctaaaaa
      361 agtgataact acttaagatg aagcagaata tttacgccat acggcataca accgcgaaaa
      421 atataaaaaa aagaaaacga ttgaacgcgt ttttgcagtc gcaaaggaaa agcatggaat
      481 gcgctacatc aagtatcgag acttggaaaa caatcaggtg tatacgctga ttgtttttgc
      541 tgcaatgaat ctgaaaaagt tgtccacttg gcagttggaa catatgtctt catatctttt
      601 attatacaaa aatagcaaaa gcaccagtct acactcttag taaagaggaa actgatcctt
      661 ttgtctacat tcttaaagta cttaaactaa gtactttatt ttactcttta ggattgatac
      721 agtagcatct atccctttat taataatctt ttattttttt aaataaagat gttgtctcca
      781 tcttcaggca ctaaaacttt ttcggaaatt cctttttctt tattgaaaga ctttaattct
      841 tctcttgaca gtccccaatg gtttacagct tccatatgaa cggcaataat tgtggaatct
      901 ggtattgctt tatgcaattc atataaatcg tctttcccca taataagaga ttctccaact
      961 aaaaaatgat tatctccagc atttgctact atgatttctg gattatactg atcaatagtt
     1021 tgtttaacag cttcatacca aactgtatct cctgtaaagt atagtgtctt ctcagtcgaa
     1081 tgtttgaaaa caataccgca aacattacct acataattca agatttctcc tctgccatgt
     1141 ttgccttttg ttttaaccaa ttgtatagat tcaaaaactg tgtcttctcg tagtacctcc
     1201 acatttttaa aaccatatag tcgaatttca ttcgcatcat tttcatcttg cacaaataat
     1261 tttatttctt taggtaggac ctctttcgct atatcgtccc aatgatctag atgtaaatgt
     1321 gttacaataa cagcatcaat accgttgata atatcttcga ctggcattgg caaatcaact
     1381 agaggattca tttgatcttt tcttggtgat ggaaaaggac ctttcgaaat aaatggtgga
     1441 agtgttcctt tttccgctaa cattggatca attaaaaatt ttttacctcc atagtctaag
     1501 atagctgttg cattacgaat gtgatgtata ttcataaacg ttgtctcctt tttgttgata
     1561 tgaaatattt ttagtataat tcaattccat acaataaata aaccgaacaa aaaaagtctt
     1621 ttactatttt tacttaattt atgaaaggtt gtttaccatg ttagaccaaa cagatataaa
     1681 tatcattgag gaactatcaa aaaatagtcg aatcacaatg aaagaacttg gagaaaaagt
     1741 acatttgact ggacccgctg tctcggcgag agtgacaaaa ctagaggaaa gtggagttat
     1801 tgaaaattat acagtaaaat taaatatgga aaagctaggg ttttcgattc acgctttttt
     1861 aattataatt acgcaaaatt taaatcataa accctattta tcatttatta aaaatcatga
     1921 aaaattcttg attagaaatt ataagattag tggagggggc tgttatcttt tggaatgtag
     1981 atttacttca aaaaaatcaa tgaacagctt tttggaacag cttaatgaat atgcgaatta
     2041 taaattatct attgttatag attaataata aaactagcag atggtaaaaa tactaatatg
     2101 cgagacacac attcaaccat aactatataa cttatcaatt taaaactaaa ctgatggaaa
     2161 caagtaaggc aaaaacattt atattttgcc ctacttattt ttttaattaa att
//
LOCUS       DANKHL010000278         2201 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000278 DANKHL010000000
VERSION     DANKHL010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.290"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            158..991
                     /gene="msrA"
                     /locus_tag="PW101_002746"
     CDS             158..991
                     /gene="msrA"
                     /locus_tag="PW101_002746"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2515114.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            1104..2060
                     /locus_tag="PW101_002747"
     CDS             1104..2060
                     /locus_tag="PW101_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2515115.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT          802 a          362 c          442 g          595 t
ORIGIN      
        1 aaaataaatt gattaaaaag gagaatctgt cgataaggat tctctctttt ttatacgaaa
       61 aatctgtagg gggatgaagt aatcgcttat ttcctcactt tatgataaat tacagctaaa
      121 gtcactagct tgtcttcttc ggaaaggagg agaatctatg gatgaggaac aaatattcat
      181 tcaaacactt tataatctga ttttgaatcc gaatacacga gattgggaac gaaaagtatt
      241 gattcagaca aagaatgaca taagagaaaa tatatccgtt aaagaacagt tatcaaaact
      301 agaagcaaca ctacgtccat tagctatccg aatgaatctg acgcctgatg taatggactt
      361 ttatttattg attacggaag gatttgataa ggagcaaaaa tatgactttt ccaagcatgc
      421 catgcaagat gctgactatc aagaacgtgc agtatttgca gggggatgtt tctggtgtat
      481 ggtcgagccg tttgaatcga aaaaagggat cctatctgtt ttgtcaggat acacgggcgg
      541 acatgttgaa aaaccaaatt atgatcaagt aagtggtggt tacacaggtc atgtggaagc
      601 tgtggagatc atttatgaca caagagaaat aagttatagc gagttgctga caatctattg
      661 gcaaattact gatcccacag atacatttgg acaatttcaa gatagaggga agcaatatcg
      721 tccagtgatt ttttatcaag gcgaacgaca aaaagaatta gcggaacaat caaaacagaa
      781 actagacagt tcaggaactt tccaccaacc aatcgtcaca aaaatcgaac cagccggtac
      841 gttttggcca gcagaaaact atcatcagca attttacaaa aaacaaccga aacgttataa
      901 aaaaatccag caagcacgta atcaatttct gatctatcaa cgagtaaaaa acaaatggca
      961 aaaaaatata cgaaaaaatc attttgatta actccttttc tttaaaatgt agtaaactaa
     1021 aaatagaaaa aagaaagcgt tttaaaaaga tttgatgagg aggagaaaaa ttgtatattg
     1081 cagatagtgc acgttatgaa aaaatggaat atcgtaaatc cggtcattca ggtttgaaat
     1141 taccgatttt atctctcgga ttatggcaaa attttggtga ttatgatcct atccataatc
     1201 aaagagagat tttaagaggc gcattcgaca tgggaattac ccatttcgac cttgcgaata
     1261 attatggagg ccctgctgga gctgcagaga aaaactttgg gcgtattttt agagaagatt
     1321 ttcaagccta ccgtgatgaa ctgattattt ctagcaaagc aggttatcat atgtgggaag
     1381 ggccatatgg ggaatggggc tctcgtaaat ccatcatttc aagttgtgac caaagtctcc
     1441 aaagaatggg gctagattat gttgacatct tctatcatca tcgcccagac cctgatacac
     1501 ctttggaaga aacagcagaa gctttgatgc aacttgtaag acaaggaaaa gcattatata
     1561 ttggtatatc caactataat ggagaagata caaaaaaaat gactgaaatt ttaaaaagaa
     1621 aggaagcacc gttcattatt catcagatgc gctacaatat gttttctcgt gcactactag
     1681 aagacgatct atctccagta ttagaagaag aagggctagg ggcaatcacg ttcagtccgc
     1741 ttgctcaagg gttgctgaca aatcgttatc tacatggtat tcctgaagat tctagagcac
     1801 atcgtaagga aataccattc ctttcagaag aacaagttgg atcaactctg gaaaaaatca
     1861 aagcactgca aacaatcgcc gtatcacgag gacaatcatt agcgcaaatg gcccttgcat
     1921 ggaatttgcg acaaaaatct gttacaagtg tactggtcgg agcaagtcgg ttaagtcagt
     1981 tacaagaaag tgttcgaatg atggataatt tagatttctc accagaagaa gaattgagaa
     2041 tcgaccaaat tctagaatag aaacgatgta atcaaacttt ttatccatat attaaaaaaa
     2101 acaaaagatg acatcaagaa tcgaatgaag atcgtctgtt attgaacatc atattttttg
     2161 tcgttcaaaa taaaaatgat cgaaggctag gagtgaattt t
//
LOCUS       DANKHL010000279         2192 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000279 DANKHL010000000
VERSION     DANKHL010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.291"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            359..814
                     /locus_tag="PW101_002748"
     CDS             359..814
                     /locus_tag="PW101_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515116.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            complement(976..2148)
                     /locus_tag="PW101_002749"
     CDS             complement(976..2148)
                     /locus_tag="PW101_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2515117.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
BASE COUNT          704 a          436 c          394 g          658 t
ORIGIN      
        1 aaaaatcccg tgtatcaaaa cgggattaga ttaagtttcc tcaatcacta ttcataacac
       61 aattattata acaagtaagt aaacaattga aaaggacatt tattgaaaac ggttgttttt
      121 tacttaagga ggaaatttag tgaaaaaatt agttgcattg tttttggtcg caggaagttt
      181 gttgagtggg tgtggtgcta ctacgtctac tactcaaagc acaagttcag ctcaggcttc
      241 cagcagcagt accacgatct ccgaaagtac ggcagattca tcgaaagaac aagaacaaat
      301 tgaaaagaaa aaagaagaag agaaacaaaa agcggaaaaa gctgcactag aaaaaaaagt
      361 gacagaagcg gaatctgctt tgtcacaagc tgaagcaaat cctacaaaag aaaactatgc
      421 aactgcttca gcagctatcc aagcgctacc tgaaaacaaa caagagttga caagccgtct
      481 tgctgcaatt gattccacaa tcaaagcaaa agaagcagaa gaagaacgga aaaagcaaga
      541 agaaattgca cgacaacaag cagaacaagc gcaacaagaa gcaatcgcac agcaacaagc
      601 agaacaggcg cgacagcaag cagcagctga acaggcctct cagcaggcac aagccgcagc
      661 tcctgagcaa aataatgaac aaactgtcta tgtcacaccg accggttcaa aataccatac
      721 acataaatgt ggaaatggta cgtatacccc tgccacttta agcgaagctc aagctcgtgg
      781 tttaacccct tgctccaaat gttatggaaa ctaattcaaa agttatacta taataataaa
      841 attcttctga aacataaaaa aatagaagct ggcgttccag atagtaaacc aaaaatagca
      901 gtttacacgt ccatttcgtc agcttctctc ttttttcatt tagtctgttc cctctgcttt
      961 tttggacatc ttcattcatt ctttctcaat caaatagtac acctcttttt cttttcgcag
     1021 tttatatctt ccttttttca atttcattga aaactgtttc gaatgtttat gttttacatg
     1081 gattttaagc aggagctcct catgttgcaa gcgatagttc ctgcctttta aatcagaaaa
     1141 tctcactagt ccgtattcat cagaagcttt cacaaatgga actttttctc tagtaatata
     1201 gtgtttgccg ttgagcgttt ttacagaaaa gaccattcct tcgattggtc gtccgtttgt
     1261 gttctttaca taaaaagata aaggatagtt tcgcttagcg atggccaaag aatctaaata
     1321 agcgatgagt agccaaacta agccgattga tccaagtagt ataccgataa accaaataaa
     1381 aaatttccat tttttccatt gatcaattcc ttggatcgct ttttttgcct cttttgcttg
     1441 ataaggaata cgatgccccg tcacaagcag tcgatgtgta ttgaccatat aaggagtaca
     1501 ggtaagcaaa gtcacgagat ctctgccttt ttggataccc aaagaatcta cttcatcagg
     1561 taagaccact tggatttttt ctacttgata cgccaatgtc tttccattga tctggatgaa
     1621 aaactggtct ccttttttca acttaggcaa atctgtaaat aattttgctt ctgggagtcc
     1681 acgatgccca gaaagaacgg catgggtgtt tttcccgcca gtcggataag aactttcttc
     1741 caataatgac gttccttttt gcaaaaatat ttccgttgtc tgatcgaata taggtaagct
     1801 gacattgatt tttgggattt cgatgactcc aagcatatgt tccataaaaa aggcattact
     1861 aggtaaatct tttaatgctt ttgcatcaac tgcttggttg aaactgccta tccctggtga
     1921 tacgttttgc tctgccaact gttggttttt ctttgtcatt ttttcctgct gtttttttat
     1981 ttcctctgaa ttcttattcg atgcttgctt ttgataatgg atgatcattt gctgggcaag
     2041 aaaatcattc agtgaatctt ctacaaaagg ataagaaaag atcccgatcc caacgactaa
     2101 aatgattgcc atgaagatgt ccagtattct tattctgctt ttattcatca tgtccaccta
     2161 ttttctaata tgtatatgaa gaggttgtga aa
//
LOCUS       DANKHL010000280         2157 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000280 DANKHL010000000
VERSION     DANKHL010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.292"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(569..934)
                     /locus_tag="PW101_002750"
     CDS             complement(569..934)
                     /locus_tag="PW101_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515118.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1010..1255)
                     /locus_tag="PW101_002751"
     CDS             complement(1010..1255)
                     /locus_tag="PW101_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2515119.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1325..1579)
                     /locus_tag="PW101_002752"
     CDS             complement(1325..1579)
                     /locus_tag="PW101_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515120.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1743..2066)
                     /locus_tag="PW101_002753"
     CDS             complement(1743..2066)
                     /locus_tag="PW101_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515121.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          816 a          364 c          376 g          601 t
ORIGIN      
        1 aattattaat tttcgtctga aaacaaaaaa agaaagcgtt attattttag tctattttca
       61 gggggggaac cttatgataa aaaaactatt tttactatca atattaacgc tagcaatggc
      121 aggttgttct gactctggct tagcaactga actgatcaca aaatcaactg aagccacgac
      181 agaggagtta aaatgtggaa acgatcacag tgactacaga ggcttaaatc gacgatgcat
      241 gggaatatta ggcagaagca attgtaagca tcgatgtagt aatcgaagaa ggtgtagaag
      301 aagcaacaga aaaatcaaaa gaaagatcaa aagaagcaat ctgtgctgtg catcgtatac
      361 agacgtcgaa gatgcggtac ttttaaacca cgtcaggaga tgttcattta tgtctaggga
      421 agttggcgat gaaccaaaac tagagtacgc ttttagtttt gcacgtgagg atgaagataa
      481 ccctaaaact tacgacattt tagcttgatc taagatggtt aaagcttttt aatgaagtaa
      541 gaggagatag aaggcttttc tagttatatt aggtaagata aattcctttt tgctccaaat
      601 ttgctaaaca ttctgctaga taaacttcat cgtgttccgg ataaatcggc acgttgcgct
      661 ctacagtagg taaatcaaaa aatgcttcgc atgatttgcc acgatacata ggatttttcc
      721 actctggtgc tgggctgtaa ccacgttttt ccatttcatc catgacaagc tgatgatatt
      781 ggaataatct ataaggagaa tagtgaaaaa cataatccac agtagcatgt ttctttcccc
      841 agccatttcc tcttaatgca cagcattccc gatgttgtcc taaaagctgt tgacgaggaa
      901 gctttgaaat caatttttca tgccataatc gcatagaaat gtctccttta tatatagatt
      961 gttaacctta gacgttaaat gaaaaaacca gcaccaaggc tggaaaaaat cacttcagtt
     1021 tgcttgctgt ttctgagcga ccaattaggt aatcaacgga aatatcgtaa aagtcagcga
     1081 tatagattaa ttcaatgatg cttggaattt cttctccttc ttcgtactta cgatatttga
     1141 ataaaggaat acttaacgcg ctagaaacag ctacttgaga taaagatcgg ccagttctta
     1201 agaaattcag acgacaacgt aaagtatcca gtaaaatagc ttcttcgata ctcatttaat
     1261 cacctttcat tatagtagaa tagttataat gactatagaa agctacacat gcaatcaata
     1321 gtactcaatg ttttttattt aaacgtatca aataaagaat gacgatcaat aaagacaaaa
     1381 ccagtcctga taattcttct ataaaaacac tatcgggttc aaagtagttg taattaaaaa
     1441 ctttccacat aaagtagata gccggaagag tcaaaatagc taaataaaac cgttctttgt
     1501 tccaattcat aagataaaca atacttgcga ccagtgcgac cgcaggcacc caaaacaaaa
     1561 ctctcaccaa cgtaatcatg taacggactc ctcaacgatt catataataa aagataacat
     1621 gttattgcat acatcacaaa tgaaatgcta ggaaaataaa agaagaatag atcaatgaaa
     1681 aatgaaaaag cagaacagtt ttctgctgat aagcaggaac tgttccgctg aatgtgtctt
     1741 atctatcgag aaaaaatcaa tggattgata tctaagtaat tcaatacata taaaaagagg
     1801 acgatcgcat tcactaaaaa ggaaagaatg atgatcccat agcgaatgga aacatattta
     1861 acaaatactt ttgtttgaat acgggcatat ctttttcctt tatagctgag catcatgaca
     1921 agaagcaaaa gggtaatcac accttgaaaa atcattccaa taaacagaat tcctgaggtt
     1981 tgataattta attggagaaa ataagctgct gccgcaacta aatcgaataa aatcgcaata
     2041 aatggcaaaa taatcactcc taacattgag aataaataaa agaataacat tttttctaaa
     2101 ataagcaaat aaaaattaaa gtaacaaaat agttatgaca caagtaaaag aaataag
//
LOCUS       DANKHL010000281         2148 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000281 DANKHL010000000
VERSION     DANKHL010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.293"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            1..1998
                     /locus_tag="PW101_002754"
     CDS             1..1998
                     /locus_tag="PW101_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDL2515122.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          735 a          391 c          488 g          534 t
ORIGIN      
        1 ttgattgcta gattgggtta tttgcagatc gtgaacggcg aagagatgga ggcgcgagtg
       61 aagtctactt ctacgattac ggtccaagaa agtactcctc gcggaatgat ctacgactcc
      121 acaggaaaag ctttagttac gaataaagcg aatcaggcca tcacatatac gagaggaaca
      181 caagttacgg ctaaagattt gcttgaaatc gcaacgaagc tgaactcgct gatcgatgtt
      241 ccggtagatg aaaatctgac agatagagat aaaaaagatt tttggctggc tgatcccaaa
      301 catttgaaag aagccacaaa aagactttcg gcaaaagaaa agcaattagg gaacagcgaa
      361 caatatagtg cgatggtcaa taaagtcaca gctgatgaga ttaatttaaa cgaggagcaa
      421 ttaaaaattg ctaccatctt taaacggatg aatagtgcat atgaattaaa cactgtctat
      481 atcaaaaact ctggtgttac tgatcaagag ttagctgttg tggcagaacg cgcttctgaa
      541 cttccaggag tttcgactgg cacagactgg acacgagaat ataatgcagc aagttcactg
      601 aaaagtattc ttggcagtgt gacgactgaa aagcaaggac ttccagcaga tgaagcagag
      661 aaatacctag caaaaggtta ttctcggaat gaccgtgtag gacaaagtta tttggaaaaa
      721 caatatgaag atgtcctgca aggaaccaaa acccaatatg aagtttccct tgataatgaa
      781 ggaaatgttt cgaatcaaaa agaaatcttc tctggggaaa aaggttctaa tttaatgtta
      841 tctatgaatg cagaattcca atcaaaagta gaagaaattt tgaaaaggaa ttatcaaaca
      901 ttgatcaaca acggcaaagc acagtattcg cctggtgctt atgcagtggc tatgaatcct
      961 cagacaggag aagtgctagc aatgactggt ttcagtcatg aacaaggttc caaagaaatc
     1021 accgagaatg ctttaggcac gattactagc gcctttgatc cagggtctgt agtcaaggct
     1081 ggtactttga cagcaggctg ggcaagtaac gcgatcagcg gaaatcaagt tttgattgat
     1141 gagccgattc gtctgcaagg tgcttcagaa aaaagctctg tctttaatcg aagcggacaa
     1201 gttgctcttg atgcagtcaa agcatcggaa ttgtcttcta atacgtatat gatcaaagtg
     1261 gcgttaaaga tgttaggact agactataca cctggaatgg gactacctag tttagatgaa
     1321 gaagctaaag cttatcagca attgagagac tctttcaaag agtttggtct tggtacaacg
     1381 actgggatcg atttgccgaa tgaatctcct gggatctcac gttctgttga ttatatgaag
     1441 aagttcaatg cggataatgg caaagaatgg tatgctccag gtaactttac cgatttggca
     1501 tttggacagt ttgacacata tacaccaatc caattagctc aatatgcttc aacagtcgct
     1561 aatggcggga aacgagtaca gccgcaccta gtcaaagcga tctatggcaa tgacgaaaat
     1621 ggaaacttgg gtgaagtgaa gaaagaaatt ggcacaacag tagaaaatac ggtcaatatt
     1681 tctgcagaaa atatggcgat cctccgtgaa ggattccacc aagttgtcca tggtaccgat
     1741 ccttacacaa cagccaaacc attagccagt gcgaaaatgg acctttccgc aaaaacaggg
     1801 actgcagaaa cggttgcgga aggtcatcca gatattgcga cagtcaatag taatatcgtg
     1861 gcatacggac caacagacaa tccggaaatt gccatcagtg ttgtcttgcc gaacttatta
     1921 gacgaacaag accacataaa cttgacgatt gctaaagaaa taatggacgc gtattatgat
     1981 atgttcatgg caaaataaaa aagaggtcgt gaaaaagccg cttagtttcg agtatgaaga
     2041 aaaattttgt gaaaatagct tctcacattt ttgcttaaat ttgacttcat accgagaaac
     2101 tggcttttga acaccgttta ctcggcaaaa aaggttgtga caaaaatt
//
LOCUS       DANKHL010000282         2125 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000282 DANKHL010000000
VERSION     DANKHL010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.294"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(88..1014)
                     /gene="cysK"
                     /locus_tag="PW101_002755"
     CDS             complement(88..1014)
                     /gene="cysK"
                     /locus_tag="PW101_002755"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDL2515123.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1154..2041)
                     /locus_tag="PW101_002756"
     CDS             complement(1154..2041)
                     /locus_tag="PW101_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL2515124.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          587 a          474 c          356 g          708 t
ORIGIN      
        1 ataatttttc tcatcataaa tttttgtgtg ttcttttcat ctttaaaagt tttttacata
       61 aacctatctg gagaaaaacc aagtgcctta tatctcttgg taaagggctg tagataaata
      121 acgttcacca ttgtcgggaa ccacagctaa taccttcttg ccttttccta attcttttgc
      181 gacttttagc gcggcagcaa ttgcagcgcc agatgagatg cctactaaaa tcccttcttt
      241 tcgcccaact tctctagctg tttccatcgc ttcgtctcca ctaatagaaa ggactttatc
      301 atacacagaa gtatccagtg ttttaggaac aaaacctgta ccgattcctt ggattttgtg
      361 aggtcccgct tctttccctt ctaaaatagc agattctgct ggttctaccc cgattaattc
      421 gattttcgga tacacacgtt ttaattctcg gccagcgcca gtgatcgttc ctcctgtacc
      481 gatacctgca acaaacgcgt ccaagccatt tactccaaat gcttgatgta tctcaggacc
      541 agtcgtcttc tcatgcacaa gaggatttgc ttcattttca aattgtaatg gtaaaaagta
      601 gccgttttct tttgccaaac gttctgcttc tctgattgat ccagaaatac catccgcacc
      661 aggagtcaaa atcaactctg cgccgtaccc tttcatcaat aaacgtcttt cgatactcat
      721 cgtctcaggc atcacgatga ttactttata tccttttgca actcctacca tcgacaatcc
      781 gattcctgta tttccagaag taggttcaat gatagtatca cctggtttca gtagaccatc
      841 gtgttctgct ttttctatca tacttaaagc aatacgatct tttacacttc ctcctggatt
      901 aaaaaactca agctttacaa aaacatctgc tgaatcttct ggtacgattt tatttaattt
      961 gacgattggc gttttgccaa tcaattcggt gactgagtta tagatagctg tcatcttcaa
     1021 ttcctccttg tctttgctct tcttacttat cataatacaa tctgttcatt tatggcgagt
     1081 gattcgtctt tgactagcaa aaaaaagaca aagatatacc atatatggta atttctttgc
     1141 ctttttatct aaatcaaacg tttagttctt gaccaactaa taaagaataa ctggacatag
     1201 acatgccatt tttcgccatt agatcatcaa cagacatgcc aaatttttgt gcaatatccc
     1261 atagcgtatc acctgacacg actgtatatg tctgaccatt atttgtattt acatctgttg
     1321 ttgaagatga tgacgttgaa gtcgtcgtag tagtcgttgt tgttgtactt gtggttgttg
     1381 tagccgaacc tgttgatggc gtatcaaaac gggttaaatt ataagtatta atcaatgagt
     1441 tcaatttggc cccataattt ggatcggtag cataacgacc tgtcaagtac gctgttgcat
     1501 cataaaaact tgttgtattg cttttccaca cgccagcata atgataatcg cccgtggaaa
     1561 aactggttga acgaagaaca ttcgcatggt cttggaagga ttcccaataa gaagaatatt
     1621 ttcggaatgg ctcattcatc gttacccatt gtccgtttac atattctttg gttgccatat
     1681 aaacgacctg tccgttatag cttcctttca caccgaatag attataattt ggcgcactag
     1741 ctaatccgga agtgccatat gcactttcca acaacgcttg agcgatcatg actgaagcat
     1801 acaagtcatt acttgcagca acttctgacg cagaccagcc gatttgatcg ataaacgctt
     1861 gaggatcagc gactgtttga gatccttgtt catctgcatg aacatgttgc ggtaccgctg
     1921 taaccatcat cggcccaact aaaagactcg ttccaattaa aggagcaatc gcttttttat
     1981 tgatcaccat ttttctttta ggtctatggt gccgggcact gcgggtcttc agctcttgca
     2041 ttcaaattcc tcctcaaaat aaaaaaactt aataaaaagt tctcttctta aaacaaactt
     2101 aattaattaa attatacttt taatt
//
LOCUS       DANKHL010000283         2123 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000283 DANKHL010000000
VERSION     DANKHL010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.295"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..859
                     /locus_tag="PW101_002757"
     CDS             <1..859
                     /locus_tag="PW101_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2515125.1"
                     /translation="ILKKIKPDMVIAYTIKPNIYGGLACRLRKVSYALNITGLGTAFQ
                     KKGVLKSLIVFLYRLAAKKSKVIFFENFENMKTFQTLQIAKQSKYKLLNGAGVDVEHF
                     SYLEYPKDEQVHFLFIGRIMKEKGVDELFEAMKNLYSEEKNCVLDVLGYFEEDYGIKI
                     EKYEKEGWLKYHGYQSDIRPFVERAHCFVLPSWHEGMANTNLESAASGRPIITSDIPG
                     CKEAVIESKSGYLCERKNSCSLYNSMNKFLKLSYEQRINMGKMGRNYMIKKFNKKKVV
                     DETIKSLFS"
     gene            866..1261
                     /locus_tag="PW101_002758"
     CDS             866..1261
                     /locus_tag="PW101_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2515126.1"
                     /translation="MFCKVCDRKEGFCGFRIDIKELAKVSDIFLFTTLQEGMPRSMME
                     AMASGLPCIASKIRGNVDLLVEGKGGYLVPAKDAELVAECLRELINNKSLRKYMGNEN
                     LSRIKEFEVSIVKKRIKEIYEEVIGESVK"
     gene            1258..>2123
                     /locus_tag="PW101_002759"
     CDS             1258..>2123
                     /locus_tag="PW101_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2515127.1"
                     /translation="MKNPMFSIIVPIYCVEKYLKDCLDSIRNQTFKNFEAILVDDGSP
                     DECPEICDYYSQIDGRLKVIHKKNGGIIDARKTGVTSAMGEYIVFLDGDDWLANTYLE
                     KYAEVITKYNPDIICSGYFASRSNKIRKRELSCSFGYYDKDKIINLIYPKLIEDEHGE
                     YFRPQLWAKAFRTTLYKEQQQVSGQVSVGEDHACTKPCIYHANSIYMMEKCLYYYRIN
                     ENSITKKPQPYYWEGPKAIGEHFEKQIDMNKYDFQDQVYRNVTHNLFNVAVSQFNKQD
                     KYLNIKKDIIQNL"
BASE COUNT          838 a          235 c          409 g          641 t
ORIGIN      
        1 tattttgaaa aaaataaagc cagatatggt cattgcttat actatcaaac caaatatata
       61 cggtggattg gcatgtcggt taagaaaggt ttcttatgca ttaaatataa ccggattagg
      121 aacggcattt caaaaaaagg gggttttgaa aagtcttatt gtatttttgt atagattagc
      181 agctaaaaaa tctaaggtca tatttttcga aaattttgaa aatatgaaaa catttcagac
      241 tttacaaatt gctaaacaaa gtaaatacaa gctattaaac ggtgcaggag tagatgtaga
      301 acactttagt tatttagaat atcctaaaga tgagcaagtt cattttttat ttattggaag
      361 aattatgaaa gagaaaggtg tagatgaact ttttgaagca atgaaaaact tatatagtga
      421 agagaaaaat tgtgtacttg atgtccttgg gtattttgaa gaagattacg gcataaaaat
      481 tgaaaaatac gaaaaagagg gatggttaaa atatcatgga tatcaatctg atattcgtcc
      541 ctttgtagaa agagcacatt gctttgtttt gcctagttgg cacgaaggaa tggcaaatac
      601 taaccttgaa tcagcagcat cagggagacc aattattaca agtgatatcc cagggtgtaa
      661 agaagcagtt attgaatcga agtctggtta tttatgtgaa agaaaaaata gttgtagtct
      721 ctataattcg atgaataaat ttctaaaatt atcatatgag cagcgcatta acatgggaaa
      781 aatggggaga aattatatga ttaaaaaatt taataagaag aaagttgtcg atgaaacaat
      841 taaatcatta tttagttaaa aagatatgtt ttgcaaagta tgtgatagaa aagagggatt
      901 ctgtggcttc agaatagata tcaaagaatt ggcaaaagtt tctgatattt ttttatttac
      961 tacattacaa gaaggaatgc caagatctat gatggaagct atggcaagtg gattgccatg
     1021 tatagcaagt aaaatccgag gtaatgtaga ccttttagtg gaaggaaaag gtggatatct
     1081 tgttccagct aaagatgcag aattagtagc agagtgtttg agagaactaa tcaataataa
     1141 atctcttaga aagtatatgg gaaatgagaa cctttccaga attaaagaat tcgaggttag
     1201 tatcgtaaaa aaaagaatca aagaaatata tgaagaagtt ataggtgaga gtgtaaaatg
     1261 aaaaatccaa tgttttcaat aatagtacct atctattgcg ttgaaaaata tttgaaagat
     1321 tgcttagatt caataagaaa tcagacattt aaaaattttg aagcaatact agttgatgac
     1381 ggtagtcctg acgaatgccc agaaatatgt gactattatt cccaaataga tggacgttta
     1441 aaagtaattc ataagaaaaa cggtggaata atagatgcac ggaaaacagg agtcactagt
     1501 gctatgggag agtatatagt ttttctagat ggtgatgatt ggcttgcaaa tacttattta
     1561 gaaaaatatg cggaagtaat tactaagtac aatccagata taatatgtag tgggtatttt
     1621 gcatcacgtt ccaacaaaat tagaaaaaga gaattgtctt gctcgtttgg atattatgac
     1681 aaagataaaa ttataaattt gatttatcct aaattaattg aggatgaaca tggagagtat
     1741 tttagaccac aattatgggc aaaagctttt agaactactc tgtacaagga acaacaacaa
     1801 gtatctgggc aagttagtgt tggtgaagat catgcatgta caaaaccatg tatttaccat
     1861 gcaaattcga tatatatgat ggaaaaatgt ttatattatt acagaataaa cgaaaattct
     1921 ataacaaaaa aaccacaacc atattattgg gaaggaccaa aggctattgg agagcatttt
     1981 gaaaagcaga ttgatatgaa taagtatgat tttcaagatc aagtatatag aaatgttaca
     2041 cacaatttat ttaatgttgc tgtttcacaa tttaataaac aggacaaata tttaaatata
     2101 aaaaaagata ttatacaaaa tct
//
LOCUS       DANKHL010000284         2107 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000284 DANKHL010000000
VERSION     DANKHL010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.296"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..425)
                     /locus_tag="PW101_002760"
     CDS             complement(<1..425)
                     /locus_tag="PW101_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDL2515128.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(534..1565)
                     /locus_tag="PW101_002761"
     CDS             complement(534..1565)
                     /locus_tag="PW101_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2515129.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          657 a          338 c          361 g          751 t
ORIGIN      
        1 ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca
       61 tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt
      121 agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg
      181 tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt cactccaaga
      241 tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca
      301 agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag
      361 ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc
      421 atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat
      481 aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg
      541 gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag
      601 atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca
      661 gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat
      721 acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc
      781 tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta
      841 aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt
      901 tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct
      961 actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc
     1021 ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata
     1081 aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt
     1141 gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac
     1201 aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt
     1261 tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag
     1321 attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc
     1381 acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat
     1441 tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg
     1501 gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg
     1561 tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat
     1621 gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta ctagctaaaa
     1681 gatatgaagt aagaagatac atttttagtt attttattag tgcagtcatt cttttgatct
     1741 tttaattgat ttatcctctt tttagctatg tggttacttc atgagcaatg aaatggattt
     1801 ggttcgttct aatggcagga ggctctttct tttatttccg aaaaattaga aaacattttt
     1861 tcagaatcga ttggctctcc atttattcaa taatggtttg tctaacgata ttgttgggat
     1921 aattcttaaa gaagtattag aaatagttgg tacttcatta ctttacatga agatttattc
     1981 tatggtagga gcaattatat tgagaattaa tcctttttta tataagttgt ttgcttattg
     2041 atggattgga taaattaaac catcacaacg agtggaaatt atatgaaatt tttcataaaa
     2101 attcata
//
LOCUS       DANKHL010000285         2094 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000285 DANKHL010000000
VERSION     DANKHL010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.297"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..>2094
                     /gene="acm"
                     /locus_tag="PW101_002762"
     CDS             <1..>2094
                     /gene="acm"
                     /locus_tag="PW101_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HDL2515130.1"
                     /translation="SLIPVHVYADAGRDISSNVTSLTVDPTNITDGGNIKVKFSFDEK
                     KQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNENIKN
                     LDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFYYKTG
                     DMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQKVYR
                     GEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQINFEN
                     HSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIPNVTF
                     ELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSSNELK
                     FSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEPVPDA
                     ETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLVVTNE
                     NKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFTNLPK
                     YRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPEKTER
                     EKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSSFAVI
                     IGLLIVTICAGIFLQ"
BASE COUNT          817 a          346 c          411 g          520 t
ORIGIN      
        1 catcactgat tcctgttcat gtctatgcgg atgcaggcag agatatcagc agtaatgtca
       61 cttcgttgac cgtggaccca acgaatatta cagatggagg aaacataaaa gtaaaattct
      121 cgtttgacga aaaaaaacaa aatatccaac caggtgatta tctttggatc aattggccaa
      181 gtgaaggaaa tattcgcggt gaaggattcc aaaaagaaat cccattaatg attgaaaata
      241 aaaatgttgg aactttaacg gtcagaaaag attctgcgca ggttgtgttt aatgaaaata
      301 ttaaaaacct agactcagta gaaggttggg ggcaatttga gatacaggcc agaaacgtaa
      361 ccgataccgg tgaagaaaat acgggaccat tcaccgtgac gagcggtgat aaaacagcta
      421 ctgtaaatgt gacaaagcca gcttctggtt cttcttctag cgttttttat tataaaacag
      481 gagacatgct acctgaagat actaagcaca ttcgatggtt tttgaatatt aacaataacg
      541 gcacttatgt tgaacaacca gttaaaatca gtgatgaaat ccaaagtgga caaagattag
      601 atccaagtac gtttgaaatt aatcaaatac acttaggtga acagaaggtt tatagaggag
      661 aagagggaat ccaacaattt ttacaagatt tccccagcgc tacatttaat ttttcagtaa
      721 ctgataatta cattgaaata actataccaa aaaactttgt caatcttagg aaaataatgg
      781 tgagttataa gacgatcata gaaaacccag aacaaataaa ttttgagaat cattcggaag
      841 cttggtttaa agaatttaat aaacctgctg ttgatggaga aagtttcaat catacagtta
      901 aaaatatttc agcttctggt ggagtcaacg gtacagttag aggcgaattg aagattttca
      961 aatatatcaa tgacacagag attggtatcc caaatgtgac tttcgaatta agacgagcag
     1021 atgaacagcc tattcaagga cagtctagta tcctgttgac ttcgaatgaa cagggagaag
     1081 ctagtatcaa aggcctgcaa gtaggagact atgttgtaaa agaaaaagaa gcacccaatt
     1141 ggattgattt tgatccacta agttcaaatg aactcaaatt ctccataaac gaaaatgata
     1201 cagagggagt cagtttacct atttataaca agaaaaaggt tacgaacatc acagcaacta
     1261 aaatttggaa cggtggaacc actccaagac cgagtattta ttttaaatta ttcagagcaa
     1321 gtacaaacaa ttgggaacca gttcctgatg cagaaacaaa acgattagac aatggaataa
     1381 cttcagtgac ttgggaagat atccaacagt atgatgatag tggaaacgaa tatacgttta
     1441 aagtgcaaga agtagatcaa aacggaaatg attatgtccc ttcaggttat caaaaaattg
     1501 aaaacgggct agtcgttaca aatgagaata aagaagtcat ttcagtctca ggacaaaaaa
     1561 catgggaaga caatgagaac caagacggca aacgaccgac gacgatcaca gtaaatctat
     1621 tagccgacgg tcaaccgatc caacacaaag aagtaagtga aaaagatgat tggcgctatc
     1681 gtttcacaaa tctgccaaaa tacagagacg ggcaagaaat catctatact gtaacggaag
     1741 acaacgtgcc cgaatacagt acgacgattg acgggtataa catcaaaaac agttatacac
     1801 cggaaaaaac aaatatatct ataccaggta gccatataac accgtggaaa aattttccag
     1861 agaagacaga gagagagaaa attagaaatc tattctctca aaaattgcaa atatttgctc
     1921 atacaggaga atccagcgaa caaagaagca attatattag taagagagct gtacctaaac
     1981 agataacaaa taaatcaaaa agcattcttc ctaaaacaag tagtaaaaaa tctagttttg
     2041 ctgtaatcat tggtttattg atagtaacta tctgtgctgg gatattctta caaa
//
LOCUS       DANKHL010000286         2064 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000286 DANKHL010000000
VERSION     DANKHL010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.298"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(45..1364)
                     /locus_tag="PW101_002763"
     CDS             complement(45..1364)
                     /locus_tag="PW101_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2515131.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="PW101_002764"
     CDS             complement(1361..2014)
                     /locus_tag="PW101_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2515132.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          688 a          312 c          161 g          903 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atgg
//
LOCUS       DANKHL010000287         2057 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000287 DANKHL010000000
VERSION     DANKHL010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.299"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            494..715
                     /locus_tag="PW101_002765"
     CDS             494..715
                     /locus_tag="PW101_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515133.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            716..973
                     /locus_tag="PW101_002766"
     CDS             716..973
                     /locus_tag="PW101_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515134.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1109..1711)
                     /locus_tag="PW101_002767"
     CDS             complement(1109..1711)
                     /locus_tag="PW101_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL2515135.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
     gene            complement(1778..1933)
                     /locus_tag="PW101_002768"
     CDS             complement(1778..1933)
                     /locus_tag="PW101_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2515136.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
BASE COUNT          678 a          462 c          314 g          603 t
ORIGIN      
        1 aaaaaaaaac gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa
       61 tgaccaataa ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa
      121 ccaaaaaaca aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg
      181 caggcgccac gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt
      241 cgctaccagt cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc
      301 cgataactaa acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag
      361 cattgatttt tttctactag tcatttcgat aatataaaat tgaccacaac acagaaaact
      421 tatattataa gcttatgtcg taagtccgac gtactcacgt aatatagacg gacgttaaat
      481 ggaaggagca taaatgagag tgataaaatc agaaaaattc actttggccg aaattatgac
      541 gaccagcgaa cttgcacaag aacttgatta caaccaagct tacgttttgc gcttagcgaa
      601 agaacatctg actgaaggaa aagaatatcg ctctgccggt agaagaaact atcttttcag
      661 cccagaagcc cttaaaaaac tagaaaaagt cataaccacc aaagaaggtg agtaaatgcc
      721 taaagaaaca actatcaacg ttgaacaact agaaaaattg ctagaatcat attccaatga
      781 ttttatcgaa ttgaccggcg gaaaagtcga agaaaccacc gacctctttg acctacttct
      841 cacaaatatt ctggaattaa actctgaaga acaaaaagag cataagggaa aactgatagc
      901 accttcctac aggaaggtgg ctaagtcggc tgtgccaact ggttttctta caaaacaacc
      961 ttatattttt tgatatcaca aaaaaatcca ttttggacct attttcggtc ataatatagt
     1021 acctactttt ggtcatagtt tcgtccgtag ttttggtcat agtgactaag actttcacgc
     1081 acaaaaaaag agctgaaatt aacagctctt atcctttatt tatttttgaa tttacacttc
     1141 caccatgacg attcgatttt atatattgcc cgataatata agctgaatta tcacaaagaa
     1201 gcggaaacca atcatcaaat ctttctgaca ttgcatagtc aattaaatct ttgatttcag
     1261 tgatattctt agaatcaaca aacgacatca tctctctaat caactcatat cgttctgcac
     1321 tcgttactga caaataacta gcaatttctg caccaccatg agcgataata tcacttttag
     1381 catattgaaa tttttctgga ttatccatat gagcaaaata tcgcaccata ccttttgcat
     1441 tagcacattt ttgcgggttt ggagaccgaa gcttttgagt tatctcacgt atttgatcat
     1501 acgtcttttt accgctaaac attatcaaaa catgccaatg aggctttttc aattcaccgt
     1561 caggattcac atctttatca tgaagtggac tctcaaccca aggaacatgt aactcatcta
     1621 aaaactccct ccaattttca ggagccgatt caggatagac tacaaacgtc caattccttg
     1681 tgcgttcatc tttagttttt ctactcgtca tgctataatt accttaattg aacaacagtc
     1741 taacagcaaa agcagtgcgc caacactaat gcttttgcta ttttttttgc tcattttcct
     1801 ttccgttagc attaacccaa attgtaataa gaccagactt actaagaccc atttgtttcg
     1861 caattttatc aagattatct gccacttcct tagtaagcgt tacaacgact ctcttttttt
     1921 cattactgat cataattcgt atcctccaac gactttttct taaaaaccat agtaacaaaa
     1981 aagtaactaa ggtgcaacag tttagatact aattagcgaa taataggcct taaacccttg
     2041 gcattactga ttttgtc
//
LOCUS       DANKHL010000288         2034 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000288 DANKHL010000000
VERSION     DANKHL010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.300"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(63..1403)
                     /gene="glnA"
                     /locus_tag="PW101_002769"
     CDS             complement(63..1403)
                     /gene="glnA"
                     /locus_tag="PW101_002769"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDL2515137.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1431..1823)
                     /locus_tag="PW101_002770"
     CDS             complement(1431..1823)
                     /locus_tag="PW101_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2515138.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          578 a          451 c          342 g          663 t
ORIGIN      
        1 gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg
       61 ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc
      121 gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca
      181 atcattacat tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc
      241 tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct
      301 gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt
      361 gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg
      421 cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca
      481 ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct
      541 gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt
      601 tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg
      661 caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt
      721 ccatgcttgc gtgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca
      781 tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt
      841 gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt
      901 tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg
      961 atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa
     1021 gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg
     1081 ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca
     1141 tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg
     1201 ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct
     1261 aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct
     1321 gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca
     1381 cctgttatct gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag
     1441 aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc
     1501 aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt
     1561 gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat
     1621 ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata
     1681 cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt
     1741 tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat
     1801 cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg
     1861 taccaattaa aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta
     1921 ttttttataa aataacaaaa aaataagaac acaaaaaagg tatctagttg attttcgtac
     1981 cagatacctt ttatttttta ttatactgta ctatccttta ttagtgtttt tatt
//
LOCUS       DANKHL010000289         1979 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000289 DANKHL010000000
VERSION     DANKHL010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.301"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..367
                     /locus_tag="PW101_002771"
     CDS             <1..367
                     /locus_tag="PW101_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL2515139.1"
                     /translation="KKQIVPKEIETWWRIEMQLRRGKATDWHAMVRESLDSFASPHYL
                     PGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLLKEESQNDELTNHLRETFKESA
                     NDLKIELDTWLLGLDVTEK"
     gene            567..770
                     /locus_tag="PW101_002772"
     CDS             567..770
                     /locus_tag="PW101_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515140.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFNLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            1330..>1979
                     /locus_tag="PW101_002773"
     CDS             1330..>1979
                     /locus_tag="PW101_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL2515141.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVDDK
                     VHPLKLSWSIDRITIVGKLNDTIAYWTQNGVVFVDFEQLMRLNENQGYLKSVGTNGWQ
                     LFDQYGEDIAYIEMLKFHPDKGRIDFNPNKIGEFLKGSMKQFIHDLFLDAHFSRADIA
                     CDILGVPDDFITQYRIVDPVSFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQER"
BASE COUNT          657 a          305 c          461 g          556 t
ORIGIN      
        1 aaaaaaacag attgtaccaa aagaaatcga aacatggtgg cgtatagaaa tgcagctaag
       61 acgtggcaaa gcaacagatt ggcatgcgat ggttcgggaa agtttagata gctttgcaag
      121 tcctcattac ttgcctggag atgtaaaacc tgttgataga attatgataa aagggctgaa
      181 tcaagatcat tcagagtggg cgtatattag cagaaatttg aaatatagat tgcgcaaatt
      241 gttgaaagaa gaaagccaaa acgatgagtt gacgaatcat ttacgagaga cgtttaagga
      301 atctgcaaac gacttgaaaa tagaattaga cacgtggctt ttgggtttgg acgtgacaga
      361 gaagtgatac cgaataagaa aagtcagatg agtgagagta aatttacgtc aatgtttaat
      421 gacgtgagtt tacgataagc gattattttg cgtgaagcaa aataaagcgt aaaaggagtt
      481 gagagcgtga cacgctttcg gctccttttt tcgttgtcac ttgtgacagc gaataccctc
      541 ggagagcatt tggaattgtg acagccatgt cacaattagg aaaatgttac tctttgtcaa
      601 agagtaacag aaaggtttac actcttttta ttagcgacag cgcgaaggag gggtttaact
      661 tggggtttag tatttcgttc aaaaagggaa caaagaatac aagcatttct cataataatc
      721 gtgagttaac tgatagccaa aaaatcaatg gtttgtctgg tagaaaataa ttagcagatt
      781 tacaagtggg aaataaaaag ctgaaagaag aaaattgcat ttcaaactac attttctaca
      841 atagctctaa aatggcatat tttgcgttct gagcggtttt agcgtttatt tcgtttagtt
      901 atcggcacaa tcgttaaaac acgttagagg agcgaaaatg gagcctgtaa ggcgactggt
      961 agcggaacga tcgtgttaga ttgtgaaaac cgatagctga atgaaataaa cagcgggtgc
     1021 gccctttcca tttcggcagg aggaggctca aggggttcgg ggaatgaaat tccctgaggt
     1081 ctttttattt ttttaggagg agtgctataa tcaaagaaaa cgagaaaaaa gagcaaaata
     1141 aaagccgagc agtgcgctaa cactctcgac tatttacgga aactacttca cctagctccg
     1201 taactcaggt tcattatacg aatattttta ggtttcttca agctagagat ttttggatat
     1261 gaatttttag ggttacgtga actctggaaa ttctttccaa aagtctcttt tttcgtctgg
     1321 aggacgaaaa tggcaaatga aaatcagttg agtgaaattc aatcaaaaat tgaaggtcga
     1381 ggggcagaaa aaagaacaac gataaatcag ctcgaaaaac ttaaccgtat ttctaacagg
     1441 cggttaagtg ttgacgataa ggtacacccc ctaaaattaa gttggtcaat cgatcgaatt
     1501 acaatcgtcg gaaaactgaa tgataccata gcttattgga cgcaaaatgg agttgtgttt
     1561 gtcgattttg agcaattaat gcgtttgaat gaaaatcaag ggtatttgaa gtcggttggt
     1621 acaaatggtt ggcaattatt tgaccaatat ggagaagata tcgcctatat cgaaatgtta
     1681 aaatttcacc ctgacaaagg tcgtattgat tttaatccaa ataaaattgg cgagttttta
     1741 aaggggtcga tgaaacaatt cattcatgat ttgtttctgg atgcgcattt ctcacgtgca
     1801 gatattgctt gcgatattct cggtgttcct gatgatttta ttacccagta tcgaatcgtt
     1861 gaccctgtgt cttttaagcc aatttatggc cgttctggca aattagaaac agcgtactgg
     1921 ggtagtcgtg ccagcgagcg acaagtgcgg atgtacaaca aaaagcttga acaggaacg
//
LOCUS       DANKHL010000290         1979 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000290 DANKHL010000000
VERSION     DANKHL010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.302"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..1363)
                     /gene="wzy"
                     /locus_tag="PW101_002774"
     CDS             complement(<1..1363)
                     /gene="wzy"
                     /locus_tag="PW101_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HDL2515142.1"
                     /translation="MFSLTLVAFTIFCAFSLNQNYWLNWLGILIYLYAICTWHWRKGD
                     SIFSLYTIFFTLFLLFSYGQCIMWAFGIGTDRGIGTTVVAYGTGIIPSESDMIMVKWY
                     SCISMFVFHIGALLFTRKTTLRKVSWYESGDADADRKFLFKIGCIISIIVVPIVFVSK
                     LLEVRIALVHGYNALYYGDYATQSGYLQIILYLFFPALICLLIGSNFSKVITRFVFII
                     FALYSLLGIMSGDRGSWLYSSIILIWAYTQYKGTFNYKKLIKWLILAVIGIYILNVIT
                     KARDGGGLSKLTLKDFTSVFDSDDSPLVDSFFEMGGTMSIITFFLISGNGIYPFANTY
                     LTSLLGSISTRMLSMFGIKFVLLADWFSQDYLGITWGTGFSMIAEAYVNGGYIGGIVY
                     IFIIGIVIGKILSNSIDNQDIENHPMKMFLAITTANIIMGFPRAASYLIVKNFVYGVL
                     IILL"
     gene            complement(1406..>1979)
                     /locus_tag="PW101_002775"
     CDS             complement(1406..>1979)
                     /locus_tag="PW101_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2515143.1"
                     /translation="ENLPYYNKTTSSKKFITACRLDKQKNLPLMIDAIGKLIHEGYEC
                     ELDIYGEGGLKTELESYINHNGLTEFVHLKEFTNKIHEKMQNAAGFVISSDYEGISNS
                     MLEALAIGVPVIATDCPVGGARMYLGDRNEIGHLVECNNLDKLTDAMRRVLLDRESVV
                     RKAQKGIKLRKELTSKNISNIWYEYLNQFF"
BASE COUNT          713 a          375 c          228 g          663 t
ORIGIN      
        1 tcaacaatat aattaaaaca ccatacacaa aatttttcac aattaaatat gaagcagctc
       61 taggaaaacc cattataata tttgctgtag ttatagccaa aaacatcttc ataggatgat
      121 tttcaatatc ttggttgtct atactattag aaaggatttt accgatgact ataccaataa
      181 taaaaatata gactataccc ccaatgtatc caccattgac ataggcttct gcaatcattg
      241 agaatccagt cccccaagtg attcctaaat aatcctgaga aaaccaatca gctaataaaa
      301 caaactttat tccaaacatt gatagcattc ttgttgatat agagcctaag agtgaggtca
      361 aataagtatt agcaaaggga tatattccat taccacttat taggaaaaaa gttataatac
      421 tcatagtccc ccccatttca aaaaaagaat caactaaagg agaatcatct gaatcaaata
      481 cagatgtaaa atcttttaaa gtaagtttac ttagtcctcc accatcacgt gctttcgtta
      541 ttacgtttag aatatatatt cctataacag ctaaaataag ccactttatt aattttttat
      601 aattaaaggt tcctttatat tgagtgtatg cccatatgag tataatagag ctatacaacc
      661 aacttcctct gtctcctgac ataattccta ataaagaata taaagcaaaa ataataaaaa
      721 caaatctcgt tattactttt gaaaagttgc taccaattaa taaacatatt aaggctggaa
      781 aaaaaagata tagaataatt tgtaaatatc cactttgtgt agcataatca ccataatata
      841 atgcattata accatggact aatgctatac gtacctctag taatttagaa acaaaaacta
      901 tcggtactac tattatagaa atgatacacc ctattttaaa taaaaacttt ctatcggcat
      961 cagcatctcc agattcatac caactaactt ttcttagtgt ggtttttctc gtaaataaga
     1021 gtgctcctat gtgaaataca aacatagaaa tacaagaata ccattttacc attatcatat
     1081 ctgattcact aggaataatc cccgtaccat aagctactac tgttgtaccg attccacgat
     1141 ctgttccaat accgaatgcc cacataatgc attgcccata actaaataat aaaaaaagag
     1201 tgaaaaaaat cgtgtataat gaaaatattg aatctccttt tctccaatgc catgtacaga
     1261 ttgcatataa gtaaatcaat attccaagcc aatttagcca atagttctga tttaatgaaa
     1321 aagcgcaaaa tattgtaaac gcgactaacg ttaacgaaaa catcaatata tttatttttt
     1381 ctttcacaca atatcctcct tttggttaaa agaactggtt taaatattcg taccatatat
     1441 ttgaaatatt tttagatgtc agttcttttc ttagtttaat tcctttttgc gcttttctta
     1501 ccacactttc tctatccaaa agtaccctac gcattgcatc tgttaactta tctagattat
     1561 tacattcaac aagatgacct atttcatttc tatcacctaa atacattctt gcgccaccta
     1621 ctggacaatc tgtagcgata actggcactc caatagccag tgcttctaac atagaattag
     1681 atataccttc ataatcagaa gaaattacaa atcctgccgc attttgcatc ttttcatgta
     1741 ttttatttgt aaattctttt agatgaacaa attctgttaa tccattatgg ttgatataac
     1801 tttcaagctc ggtttttaat cccccttctc cataaatatc cagttcacac tcatatcctt
     1861 catgaataag ttttcctatt gcatcaatca ttaagggcaa atttttttgt ttatccaatc
     1921 tacatgcagt aataaatttt ttactacttg tagttttatt ataataaggt aaattttcc
//
LOCUS       DANKHL010000291         1947 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000291 DANKHL010000000
VERSION     DANKHL010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.303"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            274..840
                     /gene="amaP"
                     /locus_tag="PW101_002776"
     CDS             274..840
                     /gene="amaP"
                     /locus_tag="PW101_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL2515144.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            853..1041
                     /locus_tag="PW101_002777"
     CDS             853..1041
                     /locus_tag="PW101_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL2515145.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1054..1611
                     /locus_tag="PW101_002778"
     CDS             1054..1611
                     /locus_tag="PW101_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2515146.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1643..1885
                     /locus_tag="PW101_002779"
     CDS             1643..1885
                     /locus_tag="PW101_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2515147.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          716 a          302 c          401 g          528 t
ORIGIN      
        1 aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt aatcttgaat
       61 aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa gcaattgtta
      121 tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg gtagcgcttt
      181 atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt agagttattt
      241 acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa aataatcggt
      301 gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat caatgcgccg
      361 tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa ttactacatg
      421 caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat catcttgcta
      481 gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga agatggcaaa
      541 ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca cgaagaagag
      601 ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca tatccatgtc
      661 aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt gatgcaagat
      721 atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt ggcagtcaca
      781 tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg agtggaatag
      841 gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat ctttgggggt
      901 cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac attgttgatc
      961 ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc aggaatagtt
     1021 gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat gaatacaaca
     1081 aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg tgaattgact
     1141 tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt agatggtcta
     1201 ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa cacagataac
     1261 gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga tctagatgtg
     1321 atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga attgatcagt
     1381 actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa tgttgcagac
     1441 atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa agtaacagaa
     1501 gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa atctgctgta
     1561 ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata agaagtaaga
     1621 agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg gcggtatcat
     1681 tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca tcgcaaacat
     1741 cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt ggggaccaac
     1801 aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag tattagtcgt
     1861 ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg aaatgaagaa
     1921 caaatttgag aaccgtagcg acactcg
//
LOCUS       DANKHL010000292         1940 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000292 DANKHL010000000
VERSION     DANKHL010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.304"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..918)
                     /locus_tag="PW101_002780"
     CDS             complement(<1..918)
                     /locus_tag="PW101_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2515148.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQ"
     gene            complement(937..1341)
                     /locus_tag="PW101_002781"
     CDS             complement(937..1341)
                     /locus_tag="PW101_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HDL2515149.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(1573..1830)
                     /locus_tag="PW101_002782"
     CDS             complement(1573..1830)
                     /locus_tag="PW101_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDL2515150.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
BASE COUNT          579 a          383 c          276 g          702 t
ORIGIN      
        1 ttgttgagat atcagtattt tctttgctag tctttcttct tctgatttaa aaaatatctt
       61 gattaactgt tttctttcat aaagagaacg actgtctaat gttggaagtg ttagctttac
      121 aggtattctt ctcaacaaag ttgataatag tttatcctca ggtggttctg atgttgcaaa
      181 tataatccag caacttgatg tgtaccagtt ttcattatca cccatccgat gaaatttcct
      241 cgtatccata aactgaaaaa gtttctcctg acattcattg ctcaatgcat gaatttcatc
      301 taaaaataag actccattat tagctaattc tgctaaaccc tttcttccat cttcggctcc
      361 tgtatatgca ccttttacat gcccaaaaaa attggacaag aaaagttctg gattattggc
      421 atattcacta caattaactg aaatgaaaga tgccttctct tctatataat tattggccaa
      481 caaatcctta tacacataat ttgctaaata gctttttcca gttccagtct cgccatgaag
      541 taaaatgggt aagccatttg gcggatagta gattgctgct ttaatttgag caattacatt
      601 tcgcagactt ccttgacttc cgataaccct ttctattcca accctaaaag agctatcata
      661 ccatttttcc gcttcctctg aagagctaaa aatttgttga tttggaactt tctccggtaa
      721 agaaaagaag attactggac gagtattgat ttttccaaat tctccatctt ttacgaattc
      781 attcaaatat tgagagacaa tattacgact aacatttaat ttttcagata ttttctgtgc
      841 tgtattgttc ccccatttat ttttaatctc agagttctct atgacataat tgacaatgcg
      901 ttcttttaat tcttccatca cagcacctcc tcgacactat tcgaattttg ccgacagtat
      961 ttttccataa atatgacttg ctaaaaaggc tttgtttaga tcattcaatt ggaacgttgc
     1021 ttgttttgct tgatccgcta agacgttgaa aagatactct ggcgaaatcg cataccccaa
     1081 aatatcaacg attgttgcaa tcagatcttc ttttgattct tcatctgaaa gtaattcacg
     1141 atacaacatc gtttgtttgc ttactgtgtc atattcctct aaggattcat ctgctaaaag
     1201 aaccacttgt tccaacaaac ggtcggataa atatttatag aaaatcagcc ccaataaata
     1261 atttttatat tctgacgcat ccattttact acggagatta tctgccgcac taaataactt
     1321 ctgatttaat tctgcaccca aaaagataca ctcctatctc ttatttaaag ttgctatata
     1381 ccctcttatt ttagcatact cagttttaga aaaagcaaaa aaataaagaa acttattctt
     1441 gataacaagc tacgtgacgt atatccctaa cacaaaaata gccatatcct ttggtttgga
     1501 tacggctatt tgatattact cattcaatta tcaaatcaca gtaggtgtga atctcatcgg
     1561 gatcggcaat atctacttct gattaggatc aaaggtgatt ttctcaataa aggtgtgata
     1621 caaagcttta acttgcttct tctcactctg gataagaaag caatccaaac tagtcaatat
     1681 ctgttggaca tctttgactt gaatcggttc cccttgttgt tgaaagatcc ctaagatttc
     1741 attctctcga atttgtaact gtcttcgttt ttcagtcaat tcatttaaac gatcttttaa
     1801 catcggaaac aattcgggac tatcttccaa agcagcttcc tgtttttgct ctaatagtta
     1861 gattttgcgt accattttct tcgtttattt tccttcgatt gctgatttta atatagtcta
     1921 aaggaaattt atatttattg
//
LOCUS       DANKHL010000293         1936 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000293 DANKHL010000000
VERSION     DANKHL010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.305"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..144
                     /locus_tag="PW101_002783"
     CDS             <1..144
                     /locus_tag="PW101_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL2515151.1"
                     /translation="IVKEMPESANDMYEKELKYFFENVVTGKENWNDLYHANEVLKIA
                     EEK"
     gene            145..897
                     /locus_tag="PW101_002784"
     CDS             145..897
                     /locus_tag="PW101_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDL2515152.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            902..1849
                     /locus_tag="PW101_002785"
     CDS             902..1849
                     /locus_tag="PW101_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDL2515153.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          673 a          262 c          387 g          614 t
ORIGIN      
        1 attgttaaag aaatgcctga atctgctaat gatatgtatg agaaggagtt gaaatatttc
       61 tttgaaaacg tagtaacagg caaagaaaac tggaatgatt tatatcatgc aaacgaagta
      121 ttgaaaatag ctgaggagaa ataaatggaa attttattta ctatttgtgg tagagctggg
      181 tctaaggggt ttaaaaataa aaatttgaaa tatttggcag gaaagcccct agtccatttc
      241 gctttagcaa tgatagatat ctatcaacaa aaacatcctg aacataattg cacgacttgt
      301 ttgaatacgg acagtaatga gttagtagaa ttgacgacta agttaaatcc gtctgtgaaa
      361 attgtagaac gtaaacctga attagctaca gatactgtag ggaaaattga agtgatacgt
      421 gattcattct tacaactaga aagagaagat aatatatttg atttaattat agatttagat
      481 ttgacttcac ctttacgtga agtttctaat cttgaagaat taatagatga acatctaaaa
      541 aatttagata aagacgtcta cttttcagtt gtttctgcaa gacgtaatcc ctattttaat
      601 atggtaaaaa gaattgaaaa tgaagttact atagttaata aatcaagata cacatctagg
      661 caacagacac ctgaagtttt tgaaatgaat gcctcaatgt attcttttaa acctgaattt
      721 ctaaaaaaaa gtaattatat ttttgatggg caatgtggga taataaaaat gagagattat
      781 ctaattttag atattgatag tgaagaagat tttaaatggt tagaattttt gtttcctaaa
      841 tttttagaag attcttcgga gcttcaagat atctataatt atttatatcg gaggtaaaag
      901 gatgagctgt tatcttatca ccggtggggc tggcttcatt ggttctacct tagccaatca
      961 tttaggagaa gaacatgtgg taatcgtcgt cgatgactta tcaatgggaa aagtagaaaa
     1021 tcttgatatg acgaaaaata tcacttttat tgagggagat gtcgctgaga aatccttgat
     1081 ggaagagatc atgaaagcta ataaatttga ttatatcttt catttagcag cagttgctag
     1141 tgtggcagat tcagttgccc gaccagtaga aacacatcgt gtgaattttg aaagtgtatt
     1201 aatgatttta gaattgatcc gcaagtatca acctgattta aaacggatcg tgtttagttc
     1261 ttcagcggct gtttatgggg atgaaccgac tttaccaaaa aaagaagagt ccgtgattcg
     1321 cccattgacg ccttatgcgg ttgataaatt cgctgcagaa caatatgtgc tagattattg
     1381 tcatttatat gatgtacccg gtagcgcagt tcgttttttc aatgtctatg gtccaaatca
     1441 aaatccgaat tctccataca gtggtgtgat ttctattctc gtagatcgct ataagaaaca
     1501 aatcgtagga gaagcaactt catttacatt atttggtgat ggaagccagt ctagagactt
     1561 tgtttatata gatgatgtaa tccaagcatt acttttagtt gcgaatgagg aagctgcact
     1621 ggggaaacaa ttcaatgttg gaacgggtaa agcaaccaca cttttggaat tgattcatgc
     1681 aatcaatgaa attctcggtg tagaattgac acttgaatac aaagaagagc gttcgggtga
     1741 tatccatgac tctctagcag atattactaa gatccagtca attggctatc aaccgaaata
     1801 tggtatttta agtggaatga ctaattattt gaaaactgaa atcaattaaa acatactaag
     1861 ataagattag agggtaaatt caggctcttt gtcaataagg actgatgagt tttgtaaaat
     1921 caaatctggg atagaa
//
LOCUS       DANKHL010000294         1906 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000294 DANKHL010000000
VERSION     DANKHL010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.306"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            21..1154
                     /locus_tag="PW101_002786"
     CDS             21..1154
                     /locus_tag="PW101_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2515154.1"
                     /translation="MDIMFLAFSLSSMITSFHISGTQAGFISTITNLGMLIGGIFFGI
                     IADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFRFIAGVGAGGEYGACMSLISE
                     SFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGWKMLYVVGVLPVLMVLFIRRG
                     LTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIMVTVQIAGYFGLMNWLPSIMQ
                     NRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPKLAFSLFLICSSLSVYLLVLA
                     HSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRATANNFIMNLGRAVGGFSSIV
                     IGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALRESLQH"
     gene            complement(1320..1742)
                     /locus_tag="PW101_002787"
     CDS             complement(1320..1742)
                     /locus_tag="PW101_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2515155.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT          497 a          327 c          389 g          693 t
ORIGIN      
        1 cgggaattgc tttagagaat atggatatta tgtttttagc tttttcatta tcttcaatga
       61 ttacttcttt tcatattagt ggtacgcaag cgggtttcat ttcaacgatc actaatttgg
      121 ggatgctcat tggaggtatt ttttttggca tcatagctga taaatatgga cgtgtgaaag
      181 tattttctca aacggtcctt ctgttttcga ttgcttcttt attgatgtat tttgcttcaa
      241 acatctactt ggtttatctc tttcgcttta tcgcaggtgt aggagcggga ggagaatacg
      301 gggcgtgtat gtccttgatt tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt
      361 cggttgctgg tattggtgcg caggtcggtg cggctttggc tgcgatttta gctgctgtga
      421 tcattccaac gtttggttgg aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg
      481 tcttgttcat tcgtcgcggc ttgactgaac caaaggaatt caaatctgta caatctgccg
      541 aaaagaaaac caaacttagc agactgtttg aatcaaaaca tcttgcttgg caaacatgtg
      601 gccttagtat catggtaact gtccaaattg ccggctattt tgggttaatg aattggctgc
      661 cttcaattat gcaaaatcga ttagggcttt cggtttctgg gtcttcattg tggatggtca
      721 gtactatttt aggcatgtct ttggggatga tgacttttgg cataatcatg gataaattgg
      781 ggcctaaact agccttttct ttgtttttaa tttgttcatc cttatctgtc tatctacttg
      841 tgttagccca tagccagtgg agtttggtag cagccgctgt gatagtcggc tactttatca
      901 atggcatgta tggtggatat ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg
      961 cgacggcgaa taatttcatt atgaaccttg gacgagctgt tggtggtttt tcttctatcg
     1021 tcatcggttt cttgatggat cattatagtt taactgccgt aatcttgttt ttgagcggta
     1081 tttacctagt cagtctgctg gtcttgacaa ctcttactgg tgttacagca ttaagggaaa
     1141 gcttgcagca ttgatagttg agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc
     1201 atctgcggta ataagaccag ccaaatcaaa aacatgagac actgtttccg tttggctggt
     1261 cgtattactt gatgcagggc agctttttca actgctaatt tccttctatc tggcggctac
     1321 tattctttga cgtccgtcat ttcaaagtac tttactaata acttagcata acgaaaaact
     1381 gtggatacac tcgtatattg agcgtctgca taatcttcca aatttttctc aggatgttga
     1441 tacatgaatt caatcaaatg ataattatat gattttctgt agaaatgaag ataaataatg
     1501 tagattggga agttctcctg cttttccata gaaaagcgcc gattattctc attcttcaaa
     1561 atgaccttat caggatattt ttggaacaat tcttctaaat ctgttagata attcaagata
     1621 gacttacttg ataccttcaa ttcgttttgt agttcttcta ttgaacacca acgttctgtt
     1681 tcaagcagta aatgaatcat tttaattttc ttttgagaaa cttttgttaa aaaagtctgc
     1741 atattttctc ctcctgtatc tactttacta tactacatac aagaaaaaaa acggatacaa
     1801 atatcatatt gtcacaccgt caatagtgca taaacctgtt attatttctc tttttttcct
     1861 aagtattcta ttgatatgaa tcatttaatc gtttattata taaatg
//
LOCUS       DANKHL010000295         1895 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000295 DANKHL010000000
VERSION     DANKHL010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.307"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            214..1218
                     /locus_tag="PW101_002788"
     CDS             214..1218
                     /locus_tag="PW101_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2515156.1"
                     /translation="MLGDSLVVLAGLSGTGKSQLVTSYAKALQLSDTQIKFISVRPFW
                     EDDSDLLGYADTVNSVYRPGDSGLIDALLEASRHKENIYMVCFDEMNLARVEHYFSQF
                     LSVLEMEPNTRKIKLYNEDLENKLYNSSTYPSTIEVGQNILFVGTINTDESTHQFSDK
                     VLDRSNIISLELVPFHEISEVNDYIQSDQKKRNQLKYDDFKNFKNSNPTNGLTQKEKE
                     MFWKLHLAINSKDKNIGIGWRILKQIDEYLKNLPVQNDLSRKEAIDIQLVQRVLSKIR
                     GSDEQLSELVGKWNENDLKQVGEFEQIINEYPESSDFKYTKKILLQKARELNLHGFTL
                     "
     gene            1202..>1895
                     /locus_tag="PW101_002789"
     CDS             1202..>1895
                     /locus_tag="PW101_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515157.1"
                     /translation="MDLPFSLKLNFGYKSEEQEYELLFFQDEASLLEYTPVKVKEFSN
                     LSFTFNSIDKHAKLYIPELDDYLIENQVKEENGDICYIPSDDNIIIYNRDQGKTVPLI
                     PGYYTLYVKIGETIFYSRFEIILKDLGTPEWEKMKIEIEKMVGGLARTFIPKKTSRRE
                     ITTSNYLMNDQLFQRILEFENNIPQAIVSLESLKKEAKYRIDKRYNWKPIGSKNLVDQ
                     KTISQLQIHPEKR"
BASE COUNT          746 a          259 c          299 g          591 t
ORIGIN      
        1 atagaaaata tctctgattc caaaatatct ccagtaagtt caattattac tgaacaaacg
       61 caaatagata tatctgagtt agaagataaa cacgctattc aacaaaatga tcacttctct
      121 gaacataatg aatttataga aagatttaat cagctcacaa ttcgtagtaa attacattac
      181 tcaaaacgag acttatggaa ttttcatact gctatgttgg gtgatagttt ggtggtatta
      241 gctggactaa gtggtaccgg taaatcacaa ttagtaacat cctatgcaaa agcacttcaa
      301 ttatcagata cacaaataaa gtttatttct gtgagaccct tttgggagga tgattctgat
      361 ttattgggct atgctgatac tgtaaattct gtctatcgtc caggagatag cggtctaatt
      421 gatgcactat tagaagcatc aagacataaa gaaaatattt atatggtttg ttttgatgag
      481 atgaatttag ctcgggttga acattatttc tcccaatttc tttcagtttt agaaatggag
      541 ccaaatacta ggaaaatcaa actgtataac gaagatctag aaaataaatt atataactct
      601 tcaacctatc cttctacaat tgaagtagga caaaacatat tatttgtagg aacaataaat
      661 acagatgaat ctactcatca attctcagat aaagttttgg atagatcaaa cattatttca
      721 cttgagttag taccattcca tgaaattagt gaagtaaatg actatattca atcagatcaa
      781 aagaaacgta atcaacttaa atatgatgat ttcaaaaatt ttaaaaactc taaccctact
      841 aatggtttga cccagaaaga aaaagaaatg ttttggaaac tccatttagc aattaattct
      901 aaagacaaaa atatcggtat cggttggaga atattaaaac aaattgatga atatttaaaa
      961 aatcttccag tgcaaaatga cttatcaaga aaagaagcta ttgatattca gcttgtacag
     1021 agagtattaa gtaaaatcag aggttctgat gaacaattat cagaattagt tggtaaatgg
     1081 aatgagaatg atttaaaaca agtgggtgaa tttgaacaaa taattaatga atatcctgag
     1141 tcttcagatt ttaaatatac taaaaaaatt cttttgcaaa aagctaggga gttgaattta
     1201 catggattta ccctttagtc tgaaattaaa ctttggttat aaaagtgaag aacaagaata
     1261 tgaactttta ttttttcaag atgaggcatc gttattagag tatacaccgg ttaaggtaaa
     1321 agaattttct aatctaagct ttacatttaa cagtatagac aaacacgcaa aactatatat
     1381 accagaatta gatgattatt tgattgaaaa tcaagtaaaa gaggaaaatg gagatatttg
     1441 ctatattcca agtgatgata atataatcat ttataatagg gatcaaggta aaacagtacc
     1501 tcttattcca ggttattaca ctctttacgt taaaataggt gaaactatct tttattctcg
     1561 atttgaaata atacttaaag atttaggtac accagaatgg gaaaaaatga aaatcgaaat
     1621 tgaaaaaatg gttggcggtc ttgctagaac ttttattcct aaaaaaactt ctcgtcgtga
     1681 aattactact tcaaattatc taatgaacga tcaacttttt caacgaatat tagagtttga
     1741 gaacaatata cctcaagcaa tagtttctct tgaaagttta aaaaaagaag caaaatatag
     1801 aattgacaaa cgatataact ggaaaccaat tggaagcaaa aatttagtgg atcaaaaaac
     1861 tatttcacag ttgcaaatcc atccagaaaa acgtg
//
LOCUS       DANKHL010000296         1847 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000296 DANKHL010000000
VERSION     DANKHL010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.308"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            700..>1847
                     /gene="ltrA"
                     /locus_tag="PW101_002790"
     CDS             700..>1847
                     /gene="ltrA"
                     /locus_tag="PW101_002790"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL2515158.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQK"
BASE COUNT          730 a          281 c          384 g          452 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaaga
//
LOCUS       DANKHL010000297         1844 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000297 DANKHL010000000
VERSION     DANKHL010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.309"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(374..871)
                     /locus_tag="PW101_002791"
     CDS             complement(374..871)
                     /locus_tag="PW101_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2515159.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(911..1720)
                     /locus_tag="PW101_002792"
     CDS             complement(911..1720)
                     /locus_tag="PW101_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2515160.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT          534 a          344 c          317 g          649 t
ORIGIN      
        1 gaaaaagctg ccctgcatca agtaataaga acagccaagt aaaaacagct tcttatgttt
       61 ttacttggct gttcttatta ctgcagatgc aagcttttga ataccgttta aggaagaggt
      121 tgtgaaatca gcgttttgac ctgagtatta gcttaacaaa cggaaaaata gttcctcata
      181 ttttgccgtt tgttaagcta ataccgaggg tcagctgatt gaacaccatt taagaaagag
      241 gtcgtgagcc tgccgtttca ctctgtgtca taaaaaagcc aatctacttt gatagaaaaa
      301 gcacgtagga ctaaaacagt tctgcgtgct ttttttattg tgtgttaaat atcgtaaatg
      361 aagatagata tttttatgat tcgacgatag agacgttgaa atctttcaac agctctaaag
      421 cggattctcc gtgttttcta ctggagacta acattggatc aacaaaaaag tctgcttcag
      481 gaaaagcact ctgcaggcct atcagattag agagtacaca caagtttgtt tcaatcccga
      541 ctacttcgat ctctttttgt tcttctttgc tttcaaataa agtttcctgg atttcagcta
      601 aagtttcagg tggaatcgta aagtaatact ttttaatcac tcgttcattt tcttgtggag
      661 aaagaagagg gatcaattgc aaatcttctc gttcttcttc tttgtccttc agttcaactg
      721 gtatatcccg tgtgaagaga atatattcat tattttggcg tgctttagct aatctttgag
      781 caatacgttc tgccaattga tctgactctg gaatataaaa ttcgctgttt ggatcaagaa
      841 tatgattttg catatcaata ataactaaca ttttctttcc tcctacattc atatcgtcat
      901 tttattcttt ttaatagaag ttttttaaaa tataagcaac accagcttct tcattcgtca
      961 gtgttacttg gtttgcaatt tcttttactt gtcgctgtgc attccccatc gcaatactca
     1021 acccgctgta attcaacata gcaatgtcgt tttcttgatc gccaaaagcc atgatgtcac
     1081 tcgattcgat tccaaggtaa tccgccagaa aaacaagtga atgagcttta gtgatacctg
     1141 taggcgtgat ttctaagaaa tgcggatcag agaatactga ttggatacca tctgcttgca
     1201 gatctctttg tagcttctgt agtttttctt cactcttatc tgttaatgct aatttaagat
     1261 agctaagaga aggatcttcc aacatattta aaatttgatc aaaagaatgg ttctgtaaat
     1321 tgaaatgttc ataataaggg tcatctttga ctagatttga ttcatcgaat gtcagctgat
     1381 catcttgggt ttctcggcta gcaatgatgg atattttcgc ttcttgagca tagaagaagg
     1441 cttttctcag tgtatcgcta tctatttttg aacgtctgag aactttcgcc atttttccat
     1501 ccttcatttc agcaatcagt gaaccattgg aagaaatcaa atagccaccg attcctaatt
     1561 cggaaaaaat atctttagca gaaatgctgt tccttccggt agcgacggtt acgattccgc
     1621 cattttcatc aaatttccga agtgcttgga tattttcttc tggtatcttt ccatttcgat
     1681 caagcaaggt accgtctaaa tcaatcgctg ctagtttcat tttatcttcc tactttcttt
     1741 ttacatatct attttcctgt taccttaaga ttaaatgaga atttaaaaaa atgaaactaa
     1801 cttgctttgt cattatgaat tttttcaaaa aacattctta agaa
//
LOCUS       DANKHL010000298         1828 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000298 DANKHL010000000
VERSION     DANKHL010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.310"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            33..236
                     /locus_tag="PW101_002793"
     CDS             33..236
                     /locus_tag="PW101_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515161.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            233..505
                     /locus_tag="PW101_002794"
     CDS             233..505
                     /locus_tag="PW101_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HDL2515162.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            506..691
                     /locus_tag="PW101_002795"
     CDS             506..691
                     /locus_tag="PW101_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515163.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            678..1025
                     /locus_tag="PW101_002796"
     CDS             678..1025
                     /locus_tag="PW101_002796"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515164.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            985..1461
                     /locus_tag="PW101_002797"
     CDS             985..1461
                     /locus_tag="PW101_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HDL2515165.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(1607..>1828)
                     /locus_tag="PW101_002798"
     CDS             complement(1607..>1828)
                     /locus_tag="PW101_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515166.1"
                     /translation="ENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIK
                     SAAQFIAADYDADLADQFINGNNFMPTTN"
BASE COUNT          686 a          260 c          361 g          521 t
ORIGIN      
        1 acactaaaat tttttcaaaa agaggaggaa cggtggtgaa agtcagagaa caattcaaga
       61 tagtgagcga cgaaggtaat gtttacaaaa taaagatcgt taatattaat gaatatcgag
      121 aagcagatca caaatatttg gttgacttat acatgaacga taaatatttg ggagaaggat
      181 tttgtggaga ttcacttttt gatgatgagc gaattttgaa ggaggaaaca gaatgatcca
      241 cgaactaaaa atactaccag aatattttga agcagtgacg agcggacgca aacaattcga
      301 gatccgcaag aatgatcgtg acttcaaagt tggtgatcag ttaatactac gtgaatataa
      361 aaaatacatt aaacatgaat tacctggact aacgagacca acatttagtt acactggtga
      421 ctcatacaaa gcagagatca cttatattac tgattacatg cagaagtccg gatatgtagt
      481 gttggggatt aaaggggtga attaaatgaa agtatacatc ttagctatca cagagggcac
      541 atggatgttc ccagtcggtt ccggaaaaat ctataaatca aaaacagctg cttataaagc
      601 atttgaaaaa tacaaaaaag aaaacggtgg cggcactaac gcaaagattc tagtggctga
      661 taactggcat gaagaaggtg aacgtaattg atttacctgt tcattttcgt ggtactaact
      721 atttttatgc tagtaatctt attcaaaaaa agatgagtaa agacgaacga aaaattctgg
      781 ccaactttta tttagtatta aaaatcgaac aaaatgtgtt ttcggaaagg agaattgtat
      841 gggaaaaaca aaatcaaaga ttaagaagaa aaaacgacgt ttggaacaaa aggcaatcca
      901 gaatggaacg gctaagaaaa aataaaaaag ctaccccttt cgagatagca gcgcaatact
      961 attctaccat aaggggtggc gtttgtgaga tttcattggt taaaagatta ccaagaacta
     1021 gatgagcaga ttctttactt aaagtggaat cttaataaaa gtaagcttga attgaatcga
     1081 tgggtctgtg gtgatttagc aaacgtccgc atcgaaaaga attcgagaac ttcttcttta
     1141 gaagagaata tccaaaagat agaaaatgaa ttggaattgt tgatcgagca aaaagaagaa
     1201 atgttattgc tgatagatag cttttctggt atcgataatc aaattgttaa gatgaaatat
     1261 gttgatcaaa tgagcttaga agatattgct gaatctgttg ggtatagctc atcgtatgtc
     1321 agacagcgac atgcagaaat tagaaagaca ttgaactttt tagatgagta tgaacacaga
     1381 caagctgacc gattgaaaaa agaaaacgaa attgattttt acaatagcga gaagtacaaa
     1441 gaacaactct ctttattcta aaattacact gttcactaaa tgttcggtca ttgtagctat
     1501 gtaaacattg ttagcagcat gatattctat tagtgtcaaa aaaatatgaa agagccaaga
     1561 tatcccaacc gttttattaa ttggtatctg tggctctttt attcttttaa ttagtagttg
     1621 gcataaagtt atttccattt ataaattgat cagctaaatc agcatcataa tcagccgcga
     1681 taaattgcgc tgctgattta ataaatgcat ccatatcatg aacatcttta ttagtatgtc
     1741 tacgaacata gtgcgtttca tcattaccaa tccaagtagc agctttggct aatctttgga
     1801 gtttaggaaa atcattcaaa taattttc
//
LOCUS       DANKHL010000299         1825 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000299 DANKHL010000000
VERSION     DANKHL010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.311"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            193..390
                     /locus_tag="PW101_002799"
     CDS             193..390
                     /locus_tag="PW101_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515167.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            496..978
                     /locus_tag="PW101_002800"
     CDS             496..978
                     /locus_tag="PW101_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515168.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1036..1632)
                     /locus_tag="PW101_002801"
     CDS             complement(1036..1632)
                     /locus_tag="PW101_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL2515169.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT          568 a          274 c          366 g          617 t
ORIGIN      
        1 aggaatatgt tttaattttt tgaaaaaaat tcttctaaat acaacagctg ttttaattgt
       61 gaaaacgaaa acagtacgct ataatgcctg taaggttgat ttttaactgt aaataaaaat
      121 caatgagtta aatctccaaa gaaatagcag tggtaatgtg gagaaaagtt ccagcagttt
      181 aggaggattt tgatgacgga agaaatgatt aatttaggag aacaatatgc atgcaaacca
      241 attggtttta caaaaacggt cattggtgaa gttgtatcaa aaatgacaaa ctgcgccgtg
      301 gtcaaagttg cccaatgtgc tgcagaggac caagaattat tagatgaaaa agcaagcatg
      361 gttgttgcaa aatatgatac atttgaataa agaaaacaat tggacgtgcg gcgtgctgca
      421 agtccaattt ctttgagaag gtaaatcagt attcttttcc tattttctgg agaagaaaaa
      481 gcgaggcgag ttgtcatggt aacattcttt acggttctat tgtttttatt cgttgttgtg
      541 atgtttttat ttctttggct ggttcgaaaa gaaattattt atcggactgt gcgaaatcga
      601 tgggtctact tggtgatacc cttcttagtg tcactagtca tttggtatac actcatcagt
      661 catcctacag cagatgaatt ggcaaaaggg atcttgtcag caatgatttt tattagtttt
      721 ttgttggaca gtagaggaat cacagaagaa ggacttgttt tgaatagctt tgacaaaaaa
      781 ggtgttcctt tttctgagat caataaaatt gtattatatc agccaaaagg ttctaaaatc
      841 gtaaaaatga attttttccg aaacggatgg cgaggaccga tgctgaaatt ttctgcatca
      901 ttagaagaat tagttccatt tttgtcccaa cgcttaaacg atgaggcaga gattgatatt
      961 atgattgatc cagaataaaa acagaggttg cgacaagatg tttgtcacaa cctctgtttt
     1021 ttatttcaaa taaaattatc caaatacgcg gaagcgatcg gcatcgttca tgtattcttt
     1081 ttcctcagcc ggtgtttctg gtgaaccgaa tactaattgc gaacgtagtt tccagtttga
     1141 agggatattc cattcttttg caactgcttc gtcaataact gggttgtaat gttgcaagtt
     1201 tgcgcccaat cctttatcaa ctaatgctac ccaagtatta gctgttgcga ttccgtttga
     1261 ttgttctgac caatctggga agttgtctgc atataattca aattgttctt gtagaccttt
     1321 taccacgtca gtatctttga agaataatac tgtaccataa ccatttttga aaccagctaa
     1381 tttgttttgt gtatttggga aagcttctgc tggagtcaat gggcgaagtg cttcttcagt
     1441 gatttcccaa agtttttcat gtgcatctcc gaaaaggata actgcgcgtg ttgattgagc
     1501 attgaaagca gttgggcttt ctttaattgc ttctttgatc aaagttgtta attcttcatt
     1561 agaaagtgaa acattacgtc ctagatgata gattgaacgg cggtttttca aagtgtcagt
     1621 aaaagttgtc ataataaaat ttctcctttg atttgcgttt tgcatttttt taattgatta
     1681 cgaaatgaag tatagcacct tacaaaaagt aagtaaacag atatgctcac aaaaaataag
     1741 cgaagttcaa aaaacaattt taataagaaa aatcgttatg taaggatttt cttagttatc
     1801 gctttcattg ttgtgtttta ggaat
//
LOCUS       DANKHL010000300         1818 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000300 DANKHL010000000
VERSION     DANKHL010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.312"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(315..398)
                     /locus_tag="PW101_002802"
     tRNA            complement(315..398)
                     /locus_tag="PW101_002802"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(362..364),aa:Leu,seq:cag)
     gene            926..1426
                     /locus_tag="PW101_002803"
     CDS             926..1426
                     /locus_tag="PW101_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2515170.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1616..>1818)
                     /locus_tag="PW101_002804"
     CDS             complement(1616..>1818)
                     /locus_tag="PW101_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDL2515171.1"
                     /translation="PENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKL
                     VWYPAWTTDKMSRYARIALGIR"
BASE COUNT          564 a          323 c          287 g          644 t
ORIGIN      
        1 tttgaacgtt taaacacatt caaaatatag taagaggtca tgcgatttat tctttcgcat
       61 gacctctttt tgagtttaag tttagtcttt attccttaac tatcgtttgc cagtatttct
      121 tgtatcagaa tctataaatc tactatttga atcaatctat taggttttaa actgtcccac
      181 cttcacacaa attttttttg atttatttga atctggttcc gttgtttaaa atttttccta
      241 tattctttta gataatatgt tattttcttt ctcatcgctc caggtgtacc ttctctttat
      301 aaaaaaacaa attatgcggc taagaagact cgaacttcca cgaggtcacc ctcactagcg
      361 cctgaagcta gcgcgtctgc caattccgcc atagccgcat cacatctttt agtttaggat
      421 tacatgtgtt ttttgtcaag aacatatttt tgttcttttt catttctgta cgccctccct
      481 tatgccatct acacaaataa ttcatccgag caactccatc atctattgag ggttggtgaa
      541 aaaatctgtt ttatccatat gatcgatttc aatgttaaac gaacgactct gggattagta
      601 actattttat ttttttcttc atttcttttt tcctcttttt ctcgattcag ctatcttctt
      661 tcatttggat attgattatt tcttctgttg attactatcc ttctggaatt ctagacgctg
      721 aagtaattca cgttttcttt cttctagaga tcataagtaa cgaatgaacg aacttcaaat
      781 aattttttta ttgtaattca tatttgtatt gttcgatgat tcttaatacg atatgacaca
      841 ataaataaaa ataggcatgg ctatttacaa caatagtgct atagtagaaa acaaatgaat
      901 aaggttactt aaagaggtga atacgatgaa actgatcaaa ctatgggaag cagatttaaa
      961 gaaagcttat cagctacaaa ctagctttgg accaaatgag agtggattta tcaacaatgc
     1021 ttatggctat acatttgctg aatttttaga ttatgtaaaa aaatgcgaaa aaacgtcttt
     1081 aggaatcgat cttcctgaga gttacgttcc cgcaaccgtc tttattttag aaagtgatga
     1141 acatgagtat gtaggaattt ttaatttgcg ccataaactg aacgaaacat taagaaacgg
     1201 cgctggccat attggttacg gcatctcccc taattatcgt aaaaaaggat atgccacaca
     1261 aggattagca ttagtactac aagaggcaaa aaaaatcggc attgatgagg cacttttgtc
     1321 tgtaaataaa aaaaatcttg gcagtcttaa agcacaacaa aaaaatgggg ctgcgattta
     1381 tcgtgaagat gagaaagaat actatacgaa aatcaaactg atgtaaaaaa gaaggattcc
     1441 aaaaggaaag ggatttgaat gtcctttatc aatagaacac atttatttaa ttaacttata
     1501 atatagcaga caaaatttta taaacaaaga aaccttttat ctcttttctt ctagaaagga
     1561 taaaaggctt ttttgttttt cctatttgct tctcatagct agtcagccaa gatttctatc
     1621 gtatccccaa cgcaatccgt gcataacggc tcatcttatc tgtcgtccat gctggatacc
     1681 agactagttt gacttctgct ttcgttactt ctggcacttc ttttaatgcg tcatggatag
     1741 attctgttaa aatatctgct aatggacagc ccatagttgt tagagtcatc ttaatgactg
     1801 tttctccatt ttcaggat
//
LOCUS       DANKHL010000301         1781 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000301 DANKHL010000000
VERSION     DANKHL010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.313"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            294..1778
                     /gene="guaB"
                     /locus_tag="PW101_002805"
     CDS             294..1778
                     /gene="guaB"
                     /locus_tag="PW101_002805"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDL2515172.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT          604 a          316 c          404 g          457 t
ORIGIN      
        1 tactcgaaac taagcagctt tttcacagcc tctccactaa acggtgttca aaagtttgca
       61 tctgcggtaa taaacccaac caattcaaaa acatgagaag ctgtttttga ttggctgttc
      121 ttattacttg atgcaggaca gcttttttac aatctctttt aatacacaca gatgtaaacg
      181 ctttatattg gtattctatg agaaagctaa cttgattcca agtagatcat tgacttaaat
      241 gaaaaaatca attattttaa tagataaact aaattagagg agtggtacta aaaatgtcca
      301 actgggaaac aaagttcacg aaaaaaggat ttacttttga tgatgtctta ttgataccag
      361 cagaaagtca cgtgttacca aacgaagtgg atatgagcgt acaattagct aaaaatatta
      421 agttgaatat tccaatcatc agtgcaagta tggatactgt taccgacagc aagatggcca
      481 ttgctatggc aagacaaggc ggactaggtg tgatccataa aaacatgacg atcagccagc
      541 aagcggatga agtaagaaaa gtcaaacgtt ctgaaagtgg cgtaatcatt gatccgttct
      601 tcttaacgcc gcagcatctt gtagcagacg cagaagaatt gatgagcaaa tatcgtatca
      661 gtggtgtgcc aatcgttgaa acacttgaaa acagaaaatt ggtagggata attacaaacc
      721 gcgacatgcg ttttgtaaca gattatcaca tgccgattgc tgatgtgatg acaaaagaca
      781 acttagtgac tgccccagta ggtacctctc taaaagatgc agaaaagatt ttgcaaaaac
      841 ataaaatcga aaaattacca atcgtagata acgaaggtcg tttaagcgga ttgatcacga
      901 tcaaagacat cgagaaagtg atcgaatttc caaatgcagc aaaagatgaa catggtcgat
      961 tgcttgtagc agcagccgtt ggtgtaacaa gcgatacatt tgagcgtgcg aatgctttat
     1021 tagaagcagg ggcagatgca atcatcatcg atacagctca tggacatagt gcaggtgtga
     1081 tccgaaaaat tcaagaaatc cgttcaactt ttgcagatgc aacattgatt gcaggaaacg
     1141 ttgcgacggc tgaagcgaca aaagctttat atgatgcagg tgtagatgtc gtaaaagttg
     1201 gtatcggacc aggatcgatt tgtacgacac gtgtggtagc cggtgtaggt gtacctcagc
     1261 tcacagctat ttatgatgca gcatcagttg ctcgtcaata tggcaaagcg attattgcag
     1321 acggagggat caaatattct ggtgacatcg tcaaagcatt agctgccggc ggacatgcag
     1381 tgatgctagg aagtatgttg gcagggacag atgaatcacc aggtgaattc gaaatcttcc
     1441 aaggacgtcg attcaaaaca taccgaggta tgggctcatt aggcgcgatg gaaaaaggtt
     1501 caaaagaccg ttacttccaa ggtagtgtca atgaagcaaa taaattagta ccagaaggaa
     1561 tcgaaggtcg ggttgcttat aaaggaagtg ttgcagatat tattttccaa atgatcggcg
     1621 gtttgcgttc aggaatgggt tatgttgggg cagccaatct acagcaatta cgtgatgaag
     1681 cgcaatttat ccaaatgagc ggcagtggat taaaagaatc acaccctcat gatgtacaaa
     1741 tcacaaaaga agcaccaaac tattcagttg aatcttaaaa a
//
LOCUS       DANKHL010000302         1773 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000302 DANKHL010000000
VERSION     DANKHL010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.314"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            81..1604
                     /locus_tag="PW101_002806"
     CDS             81..1604
                     /locus_tag="PW101_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL2515173.1"
                     /translation="MDPTVFEAEEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNRLARKRAEFKQFGKQIDLVIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEKTKGLIVTFRKPATMEKN
                     VEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEEQIGVYETVKGSLMEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHAFSNEWFVSEKELHASNLQYM
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          641 a          285 c          359 g          488 t
ORIGIN      
        1 tagaaacata tgcagagtat tcaccggaac agatcaatcg tatgatcgat caaatggagt
       61 ctgtcaaaaa agaaagttat ttggacccta ctgtttttga agcagaggaa catatccaaa
      121 aagtcattca taaaatgttc cgtccagaca atgcctatac aaaatttgat tttcgaaatg
      181 gccgtccaac gaagtctgcg atcttaacca cgagttccat tgatatggcc aaaaagtatt
      241 atcgggccat caaagagatg acgaaagaat ctgattggtt aataaaagag tttcccaatc
      301 agcctatccg tgaaggacga acgatggagg atcctgattt cccacgaatc gctattacct
      361 attcattaga agaaaatagc cgagattcaa gtgcccaaca agaagaaatg caaaaaatta
      421 ttgaggaata taatcagtat tatgatactg cctggtcact agcagacatt gagcgttata
      481 acggagacat caataatcgt ttggcacgaa aaagagcgga attcaaacaa tttgggaaac
      541 aaatcgattt agtaatcgta gtggatcgtc tattgacagg ttttgacgcc cctacgattc
      601 aaaccctatt tgtggatcgt aatctagagt atgcaggatt gatccaagcc ttttcacgta
      661 cgaaccgtac gtatcctgaa aaaacaaaag gactaattgt tactttccga aagccagcga
      721 caatggagaa gaatgtcgag gatgcgacaa aactgtattc agaagctaaa gaagaatcgg
      781 gtttgattta tccaagttac caagaatcca aaaaaagatt caagcaagca tatggtaagt
      841 taaaagaggt ttcatccatg gaatccattg atgaacatac gccacttgaa atccgtatcg
      901 agtacgtcaa agcatttcaa gaattgaatc gtgcgtatga atcgttagtg acttatgatg
      961 attacaatga tgacatggtg caatcttcaa ctttgaacaa tcaaattcaa cttttggaag
     1021 aacaaattgg cgtgtacgag accgtgaagg gttcattgat ggaggaagaa ccagaaaaag
     1081 agggagaaga tttttcaacg atcgaatttt atagtgaaaa taccactaag ttgtatgata
     1141 ttgattctac ttatattgat caattactag gaacttatgc tgcgaatagt tcagatgcac
     1201 gagaagaaat tgaaaaagct ttggctaaat tgaataaaac agaaggagtc aaagaagttt
     1261 accgacaaat tttaaatgca tttgatgtgg ggactttaga tagtaatgaa gatatctttg
     1321 tcattaaacg tcgctatttt acacaagcaa gtgataattt gattcatgcc ttttccaatg
     1381 agtggtttgt ttctgagaaa gaattacatg cttctaatct gcaatatatg ccaggtgaag
     1441 atccaattcc taatatgaaa gcaattatca acagtaagga ttacgaagga tataaagcca
     1501 aacatccaga agcgaaacca tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa
     1561 tgttagatga tgagttgatt ccacttgaaa atgaattgag gtaaacgaga agagttaaga
     1621 aaattgtacg attcaaagat agtagtaaag tggaagtgta gaaaataggg atagatgtct
     1681 ttttacttgt tcacatttta taacggagta tttaagaaag ctattgaata atgattcttc
     1741 catctttttc attcatttac cggttctgtt gca
//
LOCUS       DANKHL010000303         1757 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000303 DANKHL010000000
VERSION     DANKHL010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.315"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            103..585
                     /locus_tag="PW101_002807"
     CDS             103..585
                     /locus_tag="PW101_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515174.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            782..1672
                     /locus_tag="PW101_002808"
     CDS             782..1672
                     /locus_tag="PW101_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HDL2515175.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          584 a          301 c          361 g          511 t
ORIGIN      
        1 ctctcatgcc attatctcta aaaaaaaagt ttttccccct aattttttta gatagaaaat
       61 tgtatagtat gatgagagaa aaagtagttt gggggaacgt taatgagaag aaaagcagta
      121 atcgatcggc tttatttatt tttttggttt tttcctatcg ttttgctacc ttttacatgg
      181 gtaaatgctg ttgtacaagg tgcggagata ttaggcctga tccaaggaag cggatttgtg
      241 gtttctagga attttccagc tttgattgtt ttctatttag tgataagtgt aggtctctta
      301 ttaccaaaat ctctgatcaa tcactacaat tggcagctgc tttacttttc ttgcttatta
      361 gtatttacat ctacgttttc tttgctgcct cgcttgatta tcggccctaa tggtttgagc
      421 gagagtggat tgaaggattt agcttattcc tccatgtatt tcttgacgat caaaccatgg
      481 ttctatttag taattgcctc atttattctt gcaacatttt tttacttctg gacagaaatc
      541 aggataaaaa gtgaacgatc tgtatctata aaaaataact attgatggtg gatcatcagt
      601 aaaaaaacat atgagctgcg taaaatgcat gtttctcata tgcttttttt attattatgt
      661 aaagaatgcg aaaaacgttt acatgatttt tacataactt aactatattt ttacagcaac
      721 catacgaggg atttacagtc gactgataaa ctttagttgt aaataagaaa ggagcaataa
      781 aatgaaaaaa gtattgttta ctctaggtat gatgggaatt cttttagccg gatgcggctc
      841 aggatcagga aatacagatg gctctgccac acaaaataat tcaagtgact caaatgaaca
      901 agtaaaaatc gttgcggtag gttcgacagc attacagccg ttagtcgatg cagcacaaga
      961 aagctttgta caagaaaatc caaattatca aatctctgta caaggaggag gaagcggaac
     1021 tggtttgagc caagtcgaag ctggagcagt tactatcgga aactctgatg tatttgcaga
     1081 agaaagagac ggagttgatg cttcaaaatt agtggatcat aaagtagcag tggtcgggat
     1141 ggcaccaatc gtcaataaag atacagatgt caaagatatc accaaacagg aattgattga
     1201 tatctttaca ggaaaaatca ctaactggaa ggaagttggc gggaaagatc aaaaaatcaa
     1261 cgtcgtgaac cgtgcaaacg gcagcggtac ccgcgcgaca ttcgaaaaat ggggactgga
     1321 tggtgctacc cctgtgcagt cccaagaaca agattcatca ggaacagttc ggcagctggt
     1381 aagtcaaaca ccaggagcaa tcagctattt agctttctca tatctcgatg attccacaca
     1441 agcactaagt atcgacggtg tagaaccaaa agaagagaat gtagcagata acagctgggg
     1501 aatctggtca tatgaacata tgtacacgaa cggaaaacct tcccctgaag tccaaaaatt
     1561 cttagactac atgatgacag aagaaatcca agaaggacct gtcaaagaat tgggctattt
     1621 accaatcaca atgatggaag tagaacgtga tcacgaagga aatatcaaat aactagcaga
     1681 taaacatgcc acggtagtcg ttactattaa ggaaaaatca actatgtttc cccacatata
     1741 catttttcct ttaattg
//
LOCUS       DANKHL010000304         1736 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000304 DANKHL010000000
VERSION     DANKHL010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.316"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            156..1634
                     /locus_tag="PW101_002809"
     CDS             156..1634
                     /locus_tag="PW101_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2515176.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          638 a          237 c          308 g          553 t
ORIGIN      
        1 tccatttttt gcaatattct caaaaaaaag ataaaaaatg agaataactg atgaaaaaac
       61 aagcataatc catcataaaa tcagtatagg tgaaaagatg aactttcatc tgcatatcaa
      121 gtaattggta tgaggcattt tttgaggtgt aggatatgaa gttggattac cttatagaag
      181 aggaagatag agaaaaactg ctgctagcta gatatttaga atttgagaaa aagaattatg
      241 tagtgctgcc taagttatat gaaacattag gtttgtctaa atttaaaaca agaaattata
      301 tcatagaatt aaataaagaa tttactgaat tcaaaacaaa gccagagatc caaatttcag
      361 aagaagcaga gatccatgtc gaacgtctga atttgacgga tatcaaaaat ttaagaagaa
      421 tctactttca acgatcaaaa attgctcgac tgttaacgga gatcgtggat tcaaaagtaa
      481 cgatcgaaaa atttgcagag aatcattttt taagcatatc aagagcctat gtaaaaagaa
      541 gagaattagt ctcttttctt gaacagcatc aaataaagtt aaagaaaaac atattagttg
      601 gggaagaaat aaacatacgg aacgtattat ttggggtata tttcggggtc tttaatggtt
      661 tttctatgcc atttagagaa agcgtcatga atgaagcaaa gagtatcgtg caatattttg
      721 aatatctgtt caatttaaaa ctgtctgcaa caaaacgaat caaattcaat ttgctaacca
      781 ctattagttt gttgagggcc tatgggaatc aaccaatcgg aaaacgtttc ttttcagatg
      841 aatactttaa gagctcagaa ggaatgaaga tcatcaagga tttatcacat atttcttttc
      901 ttcatatccc taaagagcag atcattgatg agatttctta tatcatgttg tttttgatca
      961 cggaagaaga atttgaatta gatggcgcat ttggacttgc ggaagctcat tttcatgata
     1021 tccacatgat tagtcaaaaa atgtcagtgg aattgattga tcaattacca ttttcgattg
     1081 aaatctctga agaagaaaga ctatatatac gaaaagaact tcgtatgggt atgatgcatg
     1141 tcaatcgaaa aaattggata ttcgactttg agatgactag ctttacgtct aaaaagcagc
     1201 tacagttttt cttcgagacg tatccaatat tcagtacagc aatccaaaag gtcatacaag
     1261 agaataaacg ctatttcttg ggaaaagatg aacgtttcat gattcgctta ttttatgatt
     1321 atctcttttt attggtcggt atttgtccgg tatcatattt tgaagcaccg attcatgtct
     1381 gtattgattt ttcgcaagga tctgcttata ccgattacat tatttctcaa gtgaaaggat
     1441 ttaaaaatta caatattgtg attgagagtc gaatcacttc tcgtacgaat atttatttat
     1501 ctgattgttt acttgaaaaa tttaataaag aacagataat ttggaaaaat ccaccgactc
     1561 cagatgactg ggaatatttt ggaaatacca tcatcagaga aaaaaataga catttaactg
     1621 aaaaaatcag ctagtattta tggatggcag ctttgtctca catatttatt agctgattta
     1681 tgtttttttc aaagaaaagg taactagata taactagata taactagata tattac
//
LOCUS       DANKHL010000305         1735 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000305 DANKHL010000000
VERSION     DANKHL010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.317"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            4..963
                     /locus_tag="PW101_002810"
     CDS             4..963
                     /locus_tag="PW101_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDL2515177.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1152..1225
                     /locus_tag="PW101_002811"
     tRNA            1152..1225
                     /locus_tag="PW101_002811"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1186..1188,aa:Ile,seq:cat)
BASE COUNT          576 a          303 c          331 g          525 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagatcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
     1141 ggcaacaacc agtaccctta gctcagttgg tcagagcaga cggctcataa ccgtccggtc
     1201 gtaggttcga gtcctacagg gtacattaac gtagccattt gaatcgttct gtgttagaat
     1261 tttttgaaga gtattataca agctaaagct tttcttcatt gccactcaaa tgagtggctt
     1321 ttttatgtat ccttttatgg attaatgaaa ggatgtttca catagttata tttctgtata
     1381 tttgaaaagt tttactttga tttttaaata gaaagacatt tgggttaaat tgtgagatga
     1441 taataaagaa gagtttaaag cgcaccccaa gccacttccc cataagtgtg ttacgcttta
     1501 aactctttta tatttgaagc tattaaaagg cataccatat ttttgaaaaa aagtgagaaa
     1561 aaaggcttac aattggagtg gtagttaatt agtgacttat ttttgatttt atagcactga
     1621 tactataaaa tatagatatc atcatattac acaatcttaa tactaactta aaaaatatct
     1681 cctttcacaa gtatggtgat aaaattcgtt ccgggctacc tttttaggta gccta
//
LOCUS       DANKHL010000306         1715 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000306 DANKHL010000000
VERSION     DANKHL010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.318"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            59..481
                     /locus_tag="PW101_002812"
     CDS             59..481
                     /locus_tag="PW101_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515178.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            491..694
                     /locus_tag="PW101_002813"
     CDS             491..694
                     /locus_tag="PW101_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515179.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            905..1489
                     /locus_tag="PW101_002814"
     CDS             905..1489
                     /locus_tag="PW101_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2515180.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          603 a          243 c          312 g          557 t
ORIGIN      
        1 atctgttaag atttattaag aaaacgtttt cttatttttg atttcgaggt gatagacaat
       61 gaaaaataag tttattttat ctttaggtat tttaactatt ggaattagtg taactggaac
      121 tacaattcct acatttgctg atagtacaaa tgaaccaact actgtagtag ataataacca
      181 agaattaact cctttagata ggaacggaat agccattggt aacccaacta acgacttaac
      241 tgttactgaa aattatttaa tagtttctcc attacttgct cgagcagctt ctaaaacgat
      301 acgagtgaca ggaacgatga tttttgataa agttggttgg ccttctagac ctcctcaatc
      361 acggaattgg aaagaaaaaa gaaatggtta ttggtactat ggaaaattgt atttacaaag
      421 ttataaatca gctggaaaca attggattgc gacttattca ggaaccttaa ctagaaagta
      481 gggatttttt atgccagaat ttgtgaacac accaaatttt tatcaagata gcgatattct
      541 aagtaaaatt aatttaccga aagaagaatc agctttacaa gttcagcgaa ctttacagga
      601 gacacaaaaa attcaaaaat caatttctaa tctagaagat tctgaagaaa aaagaaaaaa
      661 aagagaccaa aaaacatatc aaaaagaaaa ataatgattt taaaaaaaga gtgtctagtc
      721 gacattcttt ttttctaatt gaaagtgttc ataaaatcac ccatttctgg acactttctt
      781 tttttgcttt atttttcaaa aaaacaattc atttcatgtt cataaaccca taacaatatc
      841 tatgttcagt taaactatat ggataaagtt atgttacaat aaaattaatg aaggagtgat
      901 ttttttgaaa ataggatacg cccgggtatc tactggttta caaaatttgg atttacaaga
      961 agacagccta aataaatttg gttgtgaaaa aatttttact gatcatatga gtggggcaaa
     1021 aagtaatcga ccaggattag aaatggccat tgattttgtt cgctctggcg atacacttgt
     1081 cgtttggcgc ttagatcgtt taggtagaaa tatggaagat ttgatttcaa tcgtcaatcg
     1141 actgaatgag cgtggtgtga gttttcatag tattcaagaa aatattacga tggacaaatc
     1201 aagttctaca ggacagctca tgtttcattt gtttgcggca tttgcagaat ttgagcgtaa
     1261 tttgatcctg gaacgatccg cagcaggacg agcagctgct cgtgcaagag gacggtttgg
     1321 tggtcgtcct gaaaaattgt ctgaacaaga tttggaatta ctgaagactt tagtggatag
     1381 cggtacacct attaagacta ttgcggaacg gtggaacgtc tcaagaacca ccatttatcg
     1441 ttatttggca aaaatggagg agaaaaatgg aaagacaaat tctcattagt ttttgtcatt
     1501 taatgctgaa aatttttgcc atttatctag aaaagtgaca atagtatgag aattttttgt
     1561 aagtgtctac tgataactta ttctcaaatg ttgtaaggat tttattcatt tgttaatatt
     1621 aaagaacgga actccaaaaa agtccgtact ctaatttgca gtctctcact cttttagagt
     1681 gagagttttt attagatttt caaattaatt agtaa
//
LOCUS       DANKHL010000307         1694 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000307 DANKHL010000000
VERSION     DANKHL010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.319"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            86..637
                     /gene="lepB"
                     /locus_tag="PW101_002815"
     CDS             86..637
                     /gene="lepB"
                     /locus_tag="PW101_002815"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2515181.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(720..1220)
                     /locus_tag="PW101_002816"
     CDS             complement(720..1220)
                     /locus_tag="PW101_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL2515182.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(1499..>1694)
                     /locus_tag="PW101_002817"
     CDS             complement(1499..>1694)
                     /locus_tag="PW101_002817"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDL2515183.1"
                     /translation="KKTSGALISFSVENADEVKKFAQIAKENGGTFHEVDMGMPEEEM
                     FSLEVQDLDGNTLEPVWMKM"
BASE COUNT          597 a          291 c          295 g          511 t
ORIGIN      
        1 ttatttcagc tatctagtcg catttttttg atatttttct tataataaag aaagaatgaa
       61 aaaaagaggg atggattgat agagattgac aaaaaaacaa cgctatatta atcgcttctg
      121 gcttgtattc aaatacttac ttgtttctgt attccttgcg ttcatgttgc ggggattttt
      181 attcatcccg gtaccagtgg aaggcaattc aatggaaaac gtgttgaagc aaggtgatat
      241 ggttgtgatg gaaaaatttt cggagatcag acgatttgat attgttgttt ttcaattagc
      301 agatggcacg atttatatca aacgagtaat cggtcttcca ggagaaaatg tcagctacca
      361 aaatgatcaa ttgaagatca atgggaaagt agttaaggaa ccttatctaa ctaaaaacct
      421 gaaatcagat catgcaaatg catcctatac gacggacttt acactacaag agctaacggg
      481 acaatccaag cttccggaag ataattattt tgtacttgga gataatcgaa gagtttcaaa
      541 agatagtcgt tcgtttggca cgatcaataa aactgatatt ctaggtaaag caagatttgt
      601 ctattatcca cttgatgaaa taaaatggat ccagtgagaa acaataaagc gtgcagtctg
      661 ttgagactgc acgctttatt gtttaatcca aaatcagttt actaccttag tacgtaaaat
      721 caaacagctc gtttaaacac ttttctagcc tctaaagttt tgataaatga ataaacaacc
      781 attatttgaa tagggatttg gatcatattc ctgatgaatc tacctagaaa aattactttg
      841 aaatctgcac caaacaaaac agacatcagc caagaattga taaaaaggct gactgggatc
      901 ataatcaata attgggcagc gatgacggaa ggcaggttta tctttttatg atataaaaat
      961 aagccattaa taaatccacc aaggaaagcc gtgataagaa aagcagcttg aaacgttcct
     1021 gaaccaaata tcgtgaaatt taaagtgttc gacaacattt caagcaaacc agcccagatc
     1081 ggtccatata gataacccat aagcgccata cttaaaaatc cgaatgtgat ttgaaaataa
     1141 gcaggaccaa tcgctatctt gtataagatg acatcaaggg ctaataaaag agcagaggtc
     1201 gacaacaatc ttgcattcat ttcgggcaat ctaaaaacgt cttttgcaat caaagtgttc
     1261 aaaatattct ctcctttgtc agttaataat aaaaggatac cataaaatca ccaatgtgtc
     1321 atgacacatt ggtgatttac atacactggc atctaacaat taaaaaaagg gtgagaagac
     1381 tgcttttata aactgtaaaa aagagattgt acgaataaaa aaaatagagg cggagaatac
     1441 ttatcgtatc atccgccttt gtttttttgt tataaaaaac gtcagattag ttatctgctt
     1501 acatcttcat ccatacgggt tctaatgtat ttccgtctag atcttggact tctaatgaaa
     1561 acatttcttc ttcaggcatt cccatatcta cttcgtggaa agtaccacca ttttcttttg
     1621 caatttgagc aaattttttt acttcatccg cattttcaac actaaacgaa atcaaagcac
     1681 cacttgtttt ttta
//
LOCUS       DANKHL010000308         1614 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000308 DANKHL010000000
VERSION     DANKHL010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.320"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            244..1413
                     /locus_tag="PW101_002818"
     CDS             244..1413
                     /locus_tag="PW101_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL2515184.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          588 a          228 c          258 g          540 t
ORIGIN      
        1 tattctttta ttactatgtt tgggttttat agcaaagaaa gtttaataag agtttggacc
       61 tagcaaacaa tattaatgag gtcattaaat cttattatct ttagtgatat aacgaaatat
      121 ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa tagatattta gggtagaaaa
      181 aaagaaagtc cattctgtaa aattaaagtt accacaaccg aaattttata ggaggacttt
      241 ctcatgccca attttactac agaaattatg gaaacactaa tcaataaagg tgatttagat
      301 gaattatttc gatgtcattt agaattagct gttaattcat tattgcaggc tgaattaaca
      361 gcttttcttg attatgaaaa atatgatcgt gctgggttca attcaggtaa ttcccgtaat
      421 gggaattatt cacgttcatt cagaacagag tatggagaat taaatttggt aattcctaga
      481 gatagaaatg gtaagttttc tcaacaaact ttacctgctt ataaaagaac caatgactct
      541 ttagaaacca ctattattca gctatttcaa aaaggaatta ctatgtctga aatctctgaa
      601 ttgattgaaa aaatgtacgg gcatcactat acgccacaaa ccatttcaaa tatgactaaa
      661 atagtttctg aagatattat tgcctttaaa gaaaggtcct tagaatccag atactctgtc
      721 atttttatgg atgctactca cattccttta aaaagacaaa ctgtttcaaa agaagcagtt
      781 tatattgtaa ttggcattcg attagatgga accaaagagg ttcttgggtt tagtcttgct
      841 ccaactgaat ctgcttatgt ttggaaagag attcttcaag acctaaaaga ccgtggttta
      901 aaagaggttt tattagtcgt aactgatggt ttaagtggca ttaacgatag tattcatagt
      961 gtttatccaa atgctcaatt tcaacaatgt tgtgtccata tatctagaaa tatcgctcat
     1021 aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt tcaaattagt ttatcaagca
     1081 agttcaaaag aagaagcaaa taatcaaata aggtttatga tagataaatg gaaaaagcag
     1141 tatccacgag tggttaaatt gctcatgaat cctgctatat taacctttta taatttccct
     1201 ccgtcaatca gaagaactat ttattcaact aatttgattg aaggttttaa caaacaatta
     1261 aagaaatata ccaaaagaaa agagcaattt cctaatgaag aatctctgga gagatttcta
     1321 gtatctcaat tcaacaacta taatcaaaag tttctatgcc gagttcataa aggttttaaa
     1381 gaaatacatg atacattaga atcaatgttt taacttgatc aacagaatgg gcttttccat
     1441 ttacacaaaa ttattgacgc tcccgacaaa attatgatct tgcgtaaagt tataaaatat
     1501 taataaaaat attttattaa tgaaaagctt ggtagcacgg ttattcatat gctaatttat
     1561 ttcactttat agtaagggga gttcttagta tattgttcaa acttaaattt cata
//
LOCUS       DANKHL010000309         1613 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000309 DANKHL010000000
VERSION     DANKHL010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.321"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            209..1351
                     /locus_tag="PW101_002819"
     CDS             209..1351
                     /locus_tag="PW101_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDL2515185.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            1364..>1613
                     /locus_tag="PW101_002820"
     CDS             1364..>1613
                     /locus_tag="PW101_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2515186.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALN"
BASE COUNT          560 a          222 c          334 g          497 t
ORIGIN      
        1 tatgttcatt tatctccagg tgtatgctta gcgggtggag tacatgtagg tgtaggaact
       61 caaataggaa ttggttctca atgtatccag atgaagaaaa ttggatcttg gtgtatgata
      121 ggcgcaggga gcactattgt taaggatatt ccctcacatt ccttagctta tggcaatcca
      181 gctaaaatta agaaagaagg aattaatttt gaattctaga attttattat caagtcctca
      241 tatgggagga acagaagaaa aatatgttgc taaggctttt aaagataatt ggatagcgcc
      301 attaggtgaa aatgtggata aatttgaaga aagtattaca aaatatactg gttctaaggg
      361 cggagcttct gcgttaagtt caggtacagc tgccatacat cttgccttaa gactgcttaa
      421 tgttggaaaa aacgatattg tctttgctcc ttcatttact tttatagcta cagtaaatcc
      481 tattttatat caaggggctg taccagtgtt cattgactca gaacctaata catggaatat
      541 gagccctgtt gcactagaga gcgcattaga agaagctgaa agaaacaacc aattacctaa
      601 ggcaatcatc gtagtgaatt tatatggtca aatggctgat tatgaacgaa ttttagctat
      661 tgcaaaccaa tttaatgttc cagtgataga agatgcagcg gaatcgttag gttctactta
      721 taagggaatt tcgtcaggct tatttgggac aattgggatc tattcattta acggtaataa
      781 gatcatcaca acctcaggtg gaggagctct agtgtctagt gataaaaaat atgttgagca
      841 ttcaagattt ttagcaacac aagcaagaga tcaagcaccg tattatcaac atagcgaaat
      901 aggttataat taccgtctaa gcaatatttc tgccggtatc ggacgtggac aaatggaagt
      961 attgaatgat agagtagcta aacgaagaga aattttcaat cgatataagg aagctttttc
     1021 tggaattgat agaattgaaa tgatgccaga agtggatggc atggtgccaa atcgttggtt
     1081 gtctacttta acgatcaatg attataattc tattgatcaa gtagttaacg taataaataa
     1141 actaaatgaa aaaaacattg aaacaagagc tttgtggaaa ccactacatt tgcaaccagt
     1201 atttcaaggg gtaaaaatgt attaccatga taataatctt agtgtgtcag aaaagttatt
     1261 taatagcggc ttatgcttac cttctggaag taatatgaca gatagtgaac aagaatttgt
     1321 tattgatgag tttaaaaaaa tattcaagta ggtgtgatat tttatgaaca taggaattat
     1381 tggtttaggt tccatgggga aaagaagact tagactcatt caagaacact ttaatgatat
     1441 tttactatgt ggtgttgata ctaatgaggc gagacgaaag gaagttgaaa aggaatttgg
     1501 aataccaact tataatagtc taacagaggc aaaaaatttg tttgaaataa attcagtttt
     1561 tgtttgtaca tcccctataa cccatgaatt tattataaaa gaggctttaa att
//
LOCUS       DANKHL010000310         1603 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000310 DANKHL010000000
VERSION     DANKHL010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.322"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(192..1484)
                     /locus_tag="PW101_002821"
     CDS             complement(192..1484)
                     /locus_tag="PW101_002821"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDL2515187.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          451 a          352 c          278 g          522 t
ORIGIN      
        1 ttcggaaaaa gggactgtga cacggttttt gtcacagtcc cttattctga ataaacagtg
       61 ttcaagagac agcctatcgg caataaggct aaacgtctct ttcactacct cttcaagtta
      121 ttattatcgt atagataaac acttcgactc gctctggtaa gaacgtcctg attcagtcgt
      181 cgtttttatc cttagatttg cgcccagacg ctttctaaga tgtttgtttg tgttctatca
      241 ggtccgacag aaaatgttga gatacgtaca ccaacaagct ctgagatacg acgtacatag
      301 ttacgtgcgt tttctggcag ttctgcaagt gttttgcatc ctgtaatgtc ttctgaccag
      361 cctggcaatt cttcatagat tggtttgcag cggttcaact ctttcaagct tgctggatag
      421 tggtagatta gttcgccatc taattcataa gccgtacaaa tttttactgt ttccaatcca
      481 cttaatacat cgatcgagtt taaagatagg ttggtaatac ctgaaactcg tttagaatgg
      541 cgcataacta cagaatcaaa ccagcctaca cgacgcggac gaccagttgt cgtaccgtat
      601 tctcgtccta cttcgcggat ttgttctcct gtttcatcaa ataattcagt tggaaatggt
      661 ccatcaccga cacgtgacgt ataggctttg cagacgccga caactttatt gatttttgaa
      721 ggccctacac cgcttccgat tgttacccca ccggctactg ggttagaaga agtaacaaaa
      781 ggataagttc cctggtcgat atccagcatt acaccttgtg cgccttcaaa caacacgcgt
      841 ttgcctgcat ccaaagcatc attcaagata acggaagtat ctgtgacgta ttgtttgatt
      901 tgttgtccgt attcataata ttcttcaaaa atatcgtcaa aatcaagtgg ttctgaatca
      961 aacattttta caaattgacg gtttttgtca tctagattca aacgaagacg ttcttcaaaa
     1021 atttctttat ctaataagtc tgcaatacgg atacctacac gagctgcttt atccatatag
     1081 gcaggtccga ttcctttaat cgttgtacca atcttatttt cgcccttagc atcttcttgt
     1141 aattggtcca atttgatatg ataaggcaag attacgtgcg cgcggtcaga aatacgcaaa
     1201 ttacttgttt ctacaccttt ttcatgtaaa taagctaatt ctttaaccaa tgatttagga
     1261 ttgactacaa cgccgtttcc gatcacactg atcttgtctt tataaaaaat cccagaagga
     1321 atcaaatgca gtttataagt cacaccgtta aacttaattg tgtgccctgc attgtcgcca
     1381 ccttggtacc gtgcaatcac ttcagcattt tcactgagaa agtctgtgat ttttcctttc
     1441 ccttcatcgc cccattgcgt acctactact actacagatg acatgtgaac acctcgttct
     1501 atattattta atcctgaacc attgtatcaa taatagatac atatttcaaa taaaatcgaa
     1561 tgatttttat ttttatttta taaaaacgct taaaaaacga act
//
LOCUS       DANKHL010000311         1596 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000311 DANKHL010000000
VERSION     DANKHL010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.323"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..623)
                     /locus_tag="PW101_002822"
     CDS             complement(<1..623)
                     /locus_tag="PW101_002822"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2515188.1"
                     /translation="MFDKNINSEIGKRIQEIRVSLGYTLEEFGENLTPPASKSNVSRW
                     EKGKNIPNKARLLSIAKLGGVSTEYLLTGNSQKTKKYIKYLFNEIFFDNGTFHNKFKD
                     FGKDRDRLGLYDLFLAVSKTYSLDKSPSLKDNFVEMIIDDVYKKLLQTDKSDDQLILG
                     FIYICLDEKLSNRQKSLKSINYEVFNYLGVAFGLIDSEIKSTNAGND"
     gene            787..993
                     /locus_tag="PW101_002823"
     CDS             787..993
                     /locus_tag="PW101_002823"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2515189.1"
                     /translation="MKPKDFRKFCGLSQSELAEKYGISVQAVRNKESGKTNYSPEEMI
                     ILKYLVKEKGIENITIDQIFFNEK"
     gene            1044..1325
                     /locus_tag="PW101_002824"
     CDS             1044..1325
                     /locus_tag="PW101_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2515190.1"
                     /translation="MKSEIQVILPSDLDQELRRYFYRVAKEAVDQVRIETSLSKEVFR
                     LGEACKYLSCSRSTLKKFIREKGLKVSIIGDCQFIRKEELARFIKEYEV"
BASE COUNT          590 a          207 c          281 g          518 t
ORIGIN      
        1 tcatcgttac ctgcgtttgt agatttgatt tcgctatcaa ttaaaccaaa tgcaacacct
       61 aaataattga atacctcata attgatactt ttaagacttt tctgtctgtt tgaaagtttt
      121 tcatcaagac aaatatatat gaaacccagg atgagttggt catctgattt atcagtttgc
      181 aagagttttt tatatacatc atctataatc atttctacaa agttatcttt taaagaaggt
      241 gatttatcta gactatatgt tttcgatact gctaaaaata agtcatatag accaagccta
      301 tctctgtctt taccgaaatc tttgaatttg ttatggaagg taccgttgtc aaaaaaaatc
      361 tcattgaata aatacttgat atatttctta gttttttgag aattacctgt cagtagatat
      421 tcagtagaaa caccaccaag tttagcaata cttagtagcc gcgctttgtt gggtatgttc
      481 tttccttttt cccatctaga aacattgctt ttgcttgcgg gaggtgtcag attttcgcca
      541 aattcttcaa gagtataccc taaagaaaca cgtatttctt gtattcgttt tccaatttca
      601 gaattaatgt ttttatcaaa caaaatggtt tcaccacctt catctattct attctacagt
      661 ttacactaca ttctcatata atgaaatgaa aaagtagata aaagttgaat tttacgttgc
      721 ggtatgaata aatatatgtt attcttttag tgacgaaaag ttgaaaagaa ggaggagtga
      781 ggagtcatga agcctaaaga ttttagaaag ttttgtggat tatcacaatc tgaacttgct
      841 gaaaaatatg gcatttcagt acaagcggta agaaacaaag aaagcggtaa aacaaactat
      901 tctccagaag aaatgattat tttgaagtat cttgtgaaag aaaaaggaat tgaaaatatc
      961 acaattgatc aaattttttt taatgaaaag tgacgaaaag ttgaatttga atacatgtag
     1021 tcaaattagt gggggcaaag aaaatgaaat ctgagattca agttattctt ccatcggatt
     1081 tagatcaaga attgcgaaga tatttctatc gagttgcaaa agaagcagta gatcaagttc
     1141 gaattgaaac atctctaagc aaagaagttt ttagattggg ggaagcttgt aagtatttaa
     1201 gctgtagcag aagtacatta aaaaaattta tcagagagaa aggtttaaaa gtaagcataa
     1261 tcggagattg tcaatttatt agaaaagaag aattagcaag gtttatcaaa gaatatgaag
     1321 tataaaaagt tgcgtggcgt gcttttgata gaaaaaagga agtgtttgtt tgaataatga
     1381 tgaagtatag aaaatataaa aatactttga aaaaaattaa aaggagtgtt cccgacagtt
     1441 tggcgaccac aggaaacact cgacaataaa gttctaagaa aactttattt gactacattt
     1501 taacatttat tagaaagaag aggaataaat ggaagaaata gataaattaa ttgctcagta
     1561 tgcatactca aaagatgacg tatatgctga aaaata
//
LOCUS       DANKHL010000312         1594 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000312 DANKHL010000000
VERSION     DANKHL010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.324"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            157..414
                     /locus_tag="PW101_002825"
     CDS             157..414
                     /locus_tag="PW101_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515191.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            428..760
                     /locus_tag="PW101_002826"
     CDS             428..760
                     /locus_tag="PW101_002826"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515192.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            805..1353
                     /locus_tag="PW101_002827"
     CDS             805..1353
                     /locus_tag="PW101_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL2515193.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            1360..>1594
                     /locus_tag="PW101_002828"
     CDS             1360..>1594
                     /locus_tag="PW101_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515194.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVF"
BASE COUNT          580 a          170 c          336 g          508 t
ORIGIN      
        1 aaatagaaaa aaacagactg tttttttaag aaaaactatg tcattcttta cttgaaaaaa
       61 gttgttatag gacagaaagg ataaaacaaa aacggaaaaa atatgtctta tgaataacta
      121 taagaactat tctatttttt aggagatgga tacgagatga aaaaactaat tggctatagt
      181 gtgatgtgta ttggatttgt tggtgtggga ttgtttgtat cgactgaatc tgcttctgcg
      241 gctgaatggt ataatcctat ctggtataat ccgaacattg aatgtcaaaa aggctatccg
      301 tatggaccga caaatctgaa aaatggaaaa tgtagagtaa attggaatgg cgttgtagat
      361 agtgtaatta ctggttcaat ggactctgct ctacatggct ttacgggtca ctagaaggag
      421 gaagaaaatg tctgaagaga aaaaagaaat cgttgaatca ttagtagctt taaaagattc
      481 tctttctaag attgaatatg ttgataggca agaaattcaa aaactaattg atgatactat
      541 tattgaaatc caagatgcgc gttgtgaagg tataaaaatt tctgtagctt tatctaaagt
      601 aatagaaaaa atgaaccgtt cattagcgtt taatggattg aagctagata gacaaactag
      661 tctgatatgg gatcatttaa aagacttgta tgacaagtct aaaagaagtg aacggactgc
      721 tgtatcaatc ttaaaaggcc tttggggaat gaattcataa acaagatatt taaaaagtaa
      781 cttatttttt atggaggaga aattatgaaa agaataatta aaatatttgg tgttttaggt
      841 gttttcttat taggtttaag tacttttgtt agtactggaa caagtgtgta tgccacagaa
      901 aaagtgaata cagttgattt agaaataaag aataaattag tagatgaatt aactttttat
      961 tttgatacag ttggtttttt agacgaacat ggtgtatacc atataaagga ttttggtgct
     1021 attcgtgaac gaatcagtca agataacgaa caaggaagag cagcaagagt cttttatgaa
     1081 catattattg ttcctagaat ggagaaagtt gattggaaac gatatgctgg atgtgtagcc
     1141 attaattctc ttcctggagg aggagtggct tgggctatag gtacgcaggg agattttatt
     1201 gaagcattaa aagcttttaa ttttgaaaaa gctgcggatt tattattaaa aattgcagaa
     1261 aaacaattag gaaaagctgc agctaaagag ttagctaagg taaatattgt agcaggaatt
     1321 gcagcagctg ctgctagctg tgcattagag taaaagttta tgaaaagttt agaacaagag
     1381 catatggctt ttaaacaagc aatgtttaaa gaaaatatct atttaaacca taactatatt
     1441 cgtgtttcta aggcatgttc accagtattg aatatgcttg gaggtggaaa tggattatat
     1501 catttactgt ttgtcgatgt ttgttggtta atttttttac cagatgaact agttatagtc
     1561 aatgaaaaaa taacaagtaa aaatgaagtg ttta
//
LOCUS       DANKHL010000313         1561 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000313 DANKHL010000000
VERSION     DANKHL010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.325"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..233
                     /locus_tag="PW101_002829"
     CDS             <1..233
                     /locus_tag="PW101_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2515195.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
     gene            392..>1561
                     /locus_tag="PW101_002830"
     CDS             392..>1561
                     /locus_tag="PW101_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2515196.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSN"
BASE COUNT          501 a          301 c          289 g          470 t
ORIGIN      
        1 tattaaaaaa tcttcctgaa acacttgatg cacaacttcg cacgaaactt caaaaccttt
       61 taacgtatga agaaggtatt tacaatgcta tgatttaccc gtattctaat ggcaaaatag
      121 aggccaagat tccccatatc aagacactga aacgattatc ttatggattt aaatcatttg
      181 agaacatgaa aatcaggatt tttttgatca atcaattgat tcaagtgaaa taacaaaaaa
      241 atctgagaca gaaggtcagt ccttctatcg gctctatact ttttgatgtt ggtgaacttc
      301 accaacatct tttttgtata aaaaaatata gaaaagacac cctgaaatcc tttagaatta
      361 atttggctac caattcatag gaggattcaa gatgtctcat actcagctta tcaaagatac
      421 cctaaatatt ctagacctaa atatctattt cgaggaaaat tgtttaacta aagaaaaata
      481 caaagggcaa atttgcttga tttacaaagg aactcttctg tacaagccag aagagtgttc
      541 ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa ccgttcggtt
      601 gttactgaat gatgtgtctg agtatcgcac ttatctggag ctgaagaaac aacgatttaa
      661 atgtaagtct tgccaaagaa cattcgtagc ggatacctct gttgctgaaa aacactgttt
      721 cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta aaagagaata cttccatgac
      781 ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc tatcgtgtca tgaagcgatt
      841 ctaccgccca ttgaatcctt tcaaacagac tttgccaaag gtcttatgct ttgatgaatt
      901 caaatcggtt cgaagtgttg ccagtgcaat gagttttatc atgatggacg gacaaagcca
      961 tgcattattg gatattgtcg aaaatcgacg cttgccttat ttagaacgct atttttctcg
     1021 gttttcatta gcgactcgtg aagccgttca attgattgtt attgatatgt atgcgcctta
     1081 tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg atcatcgatc gtttccacat
     1141 cgtccaacat attggcagaa ctttcttgaa tcatcgagtg aaagaaacca cggtacgact
     1201 taaaggaaat tcccactctc aacgagggtt agggaaaaaa ttgaagcgct attggaaact
     1261 cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg gtttggcgtg ccagttttaa
     1321 agattatttg actgaaagtg agattgccga tcgactgtta gctgcttgtc ccaacttacg
     1381 ccaaggctat cagttatacc aagacattct ttatgcggta aagaaaagag atcagtcact
     1441 atttgaggat tgtttaacta gagacattag cggtctgcca gaaacctata caaccacttt
     1501 acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg cttcactaca gctattcaaa
     1561 c
//
LOCUS       DANKHL010000314         1558 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000314 DANKHL010000000
VERSION     DANKHL010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.326"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..841)
                     /locus_tag="PW101_002831"
     CDS             complement(<1..841)
                     /locus_tag="PW101_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDL2515197.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRI"
BASE COUNT          500 a          312 c          266 g          480 t
ORIGIN      
        1 ctattcttga gctggctgca gctagtgagt gtctttcatt tatccataag ataaagcttg
       61 gattcaataa ataaataaat gttttggtta aagctgtgac cagcaaatga ggaaccgtgc
      121 gagccagttg atctgccaat gcaaacattt ctttaggtga aggcgctggc gtgtctgcca
      181 acggatactc cagtcccaat gcttggtaac cgtgcatacc atagcagtgg tacggtagta
      241 attctaacgg tacgaaccga tcaagatctg ccacaaaaca agcaatcgtc agcagctcgg
      301 tcaggttcat attgatacct ggaaccacag gaactcgaat tcgcacttcg cagttaggat
      361 actcaattaa aaagcgacga atattcttca aaatttgttg gttatctaag ccggtccatt
      421 gtttgtggag cttgctggta aacattttta catccacaaa caatgtatcc acatagggag
      481 ctaccttttg gatttcatta taagcaccac aaaagctggt ttccagtacg gtattgattc
      541 caatgtattt cgatttttgc aaaatttctt tagcaaaatc cgcctgaacc agtgcctcgc
      601 ctccgctaat ggtcactccg ccatccgagt gaaagtaaaa aacagcatcc ttctcaatct
      661 catccattac ctcttcagcg gtcatggtct ggccaaatcc tttttcaatc tgcttgcttt
      721 gtgattcggg aatcgaacac caggcacagc gtaatgggca gccctttaaa taaacaacgg
      781 tccgtaaacc agcaccatca tgtagagaac cctgttctat tcttaatact gttccttcca
      841 tatagatttt tgaattacac actagtttaa actaaaacac acaaggaaaa acttaccatt
      901 taatcataaa attagctcat ataaatatac taggatgtaa aatagactgg tcaaaatctg
      961 gtcaaaaaag aggactaata caggtagtcc tcttttttgt tattttaata ttctaaatca
     1021 ttttttattg gtttattccc ttattcgatg tagactcaaa tcactatatc actagatatc
     1081 aaacggcgtc atacggaatc aaaaacatca aatttgacct gttaaatggt cattgaataa
     1141 cattcaaaat caataaaacg gagtcagaca cggagtcaaa aacgctgaga gtaaaaaata
     1201 tatacagcct tgtaggtaaa gtaccacagg gctgtatatt ttttatttca gattacctta
     1261 aatgattcca tttacttttt atctcatttt ttcctactag ttagtatcaa atactctcct
     1321 attttctcaa taaactattt ttggatacat ggtatctgaa acagctaaaa ccttgtcatt
     1381 acagcaaaaa atcacgttat ccagactgta gacaaagtcc attttatgga tgatgtctac
     1441 agtcttttct tttataatag atttatgaaa acgaatgcaa ggagtgatag ctatgatgtc
     1501 taaaaacaat acgaatggta gaaatcaatt tgcgatgctt acgattgatg atctagtt
//
LOCUS       DANKHL010000315         1540 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000315 DANKHL010000000
VERSION     DANKHL010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.327"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(143..457)
                     /locus_tag="PW101_002832"
     CDS             complement(143..457)
                     /locus_tag="PW101_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515198.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            743..982
                     /locus_tag="PW101_002833"
     CDS             743..982
                     /locus_tag="PW101_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515199.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1344..>1540)
                     /locus_tag="PW101_002834"
     CDS             complement(1344..>1540)
                     /locus_tag="PW101_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515200.1"
                     /translation="VIGEVISKLENCVVICVDEYHDVDRNEILEKCGKVVAKYTHVYE
                     LETTEYLYNYSNKYEHTLVS"
BASE COUNT          536 a          273 c          210 g          521 t
ORIGIN      
        1 taaactctag agaaattaat tcatcctaac ataaaataat tgttcactca aaagtttttc
       61 tagaacaatt aaaacagcag gcttcacatt cgctatacat ttgtaagaag taataattcc
      121 tgtactaaaa ggcataattg ctttaattat tttttgactt ctcttcaaac acaagaagta
      181 gttgtgtaat aattgcagaa ataaggattc caataataga tataaaattt gacatggaaa
      241 cgtctataac attaaaagaa taaagtgttg aagaaagtaa actgattgaa ataacccctc
      301 cgtaaaaaat agtctgtgaa tatacccact ttttttcaga agacgtactt cgtttggttc
      361 taaaacctat ccctgcattt ctactcttat caaaaaacca aatatttaga agtaataata
      421 ttgctattat taagataaaa ttactgtaaa aatccaaaag ctatcctcct agcaactatt
      481 taaataccca aaaaaattac ctctagataa tatcatacct acgttttcac ttataaattt
      541 tttcataaaa aaagcgttta gaggcataat cgttaataca gatggtaacc aatgtatctg
      601 gtttgaactg ggatatcacc ctctccatat atgtattact aagactaata gatactataa
      661 aagacttgat agttttgcat tgattttgcc taatactgag cataggtgta tgctaaacga
      721 tactataagg agtgtgtaca taatgaaaag aattctgttt atcagtttat caaatttatt
      781 gttacttact gcttgtggga cttctgcatc aaagaacata ccttcagata cgcaaatttc
      841 gaatagtatc gattcaacaa tttctacaaa tgagaaaaat tctccggtag aaacttccga
      901 tataaaagtt tctacctctg caaaaaacag ggtaaagaaa ctgtcagcac gtctccttta
      961 tttcaaacaa actctgaatt agcttcttca tcttcttcag caaataatga tgatagtttc
     1021 gcgtttactc ctaactaaat gcaaccttta caaatgcttt acgataacaa gcctgagcta
     1081 aatgatcctg atatcagttt tgcatttttt gaaatgattg gatcggatga tctttttaaa
     1141 actactagtt tgtctatccg aaaataggac agttctggca gtataggttt ttatcgagtt
     1201 tctccaccag gcctcgttac agagacagat tctctaggcg aaccttttga ataaatcaaa
     1261 aatcatgaac aatcactgcc aactaaataa agtgccttca aaagcttggt cttctcgctt
     1321 ttgaaggcac tttatttaaa tctttatgaa actaaagtat gttcatattt gttgctatag
     1381 ttatatagat attctgttgt ttccaactca taaacgtgag tatattttgc cactactttt
     1441 ccgcattttt ctaatatttc attgcgatcg acgtcatgat attcgtctac gcagatcaca
     1501 acacaatttt ctaatttact gattacttct ccaatcactt
//
LOCUS       DANKHL010000316         1539 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000316 DANKHL010000000
VERSION     DANKHL010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.328"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(52..1518)
                     /locus_tag="PW101_002835"
     CDS             complement(52..1518)
                     /locus_tag="PW101_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDL2515201.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          502 a          326 c          289 g          422 t
ORIGIN      
        1 aaaaaacagg aactgctgca aataagcaat tcctgttttt atgttcattg attaatgaat
       61 agagcccatt agttttttga ttggttcttt cacagtaaat agcaagatac caccgacgat
      121 tgctactgca ccgataatac cgaagtaagc agattctgtt cccggtgtgt agaagcgtgc
      181 aatttgtgca ttgattgctt gtgaagaagc atcagctaag aaccaaatag cgattgtttg
      241 tgattcaaat gctcgtggtg ctaattttgt tgtcacagac aaaccaactg gtgaaagaca
      301 caattcagca ataaccacga tgaagaaact tcccaacaac cacaatggac ttacttttcc
      361 acttgtacca taaagcatac ccggcaacat caatagcaag tacgataatc ctgcaaataa
      421 aagtccaaga gagaatttca ctactgtaga tggttgacgt tcaccaagtt tcgtccacat
      481 cgttacgaat actggtgtca gaataaagat gaaaattgga ttcaatgatt gataccagct
      541 tgcttggatc gttacaccaa atagcgtaga ttgtgtacgt tcactagcaa ataaagctaa
      601 gatagatgaa ccttgttctt gtaatgacca gaaaatgatt gctgcaagga ataatgggat
      661 ataagcccat actcttggtt tttcttcagc agttacatct tttgaagtca gcattttgat
      721 aaagtaatac aacggtaaga taatccccaa tacactgatt gtattgacaa aaaagtcaat
      781 tgtcagatga ccagttagtt gtgccccgcc gaatactgca gcgataacaa caagtgcgat
      841 gatcaagtta cggatgaatc ctttacgttc ttcagttgtc aaaggattcg gcggtgttgt
      901 acccactccg tctaatgtct tgcgtccttg gatgatgtat tgtaataatc caaagaacat
      961 cccaatagca gctaaagaga accctaaatg atagttatat tcttgaccga ttgtaccaac
     1021 gattagtggt gctaataatg cccctaagtt gacacccatg taaaaaatcg aaaatcctgc
     1081 atctcttcgc aagtcagtct ttgaataaag atgtccgacc atgccagaaa catttggttt
     1141 caaaaaggcg gttccgataa taatcaatgc catcgaaaca aacaatgtac ttacaccaaa
     1201 tggtaaagcc aagacgatgt ggccaagcat aatgaaaact ccaccgaaga atacagtgcg
     1261 ttttgctcct aacactcggt cagataccca tccgccgacg atactagaca tgtagatcaa
     1321 agatccgtag atcgacatga tagctaatgc tgttgctcgt ggtaatccaa gtcccccgtt
     1381 ggcaactgta tcgtacatat aatagataag taatgcacgc ataccgtaat aactaaatcg
     1441 ttcccacatc tcagtgaaga acaaagttga caatcccttc ggctgcccga agaagccctt
     1501 gtcttgctgt ttttccataa tctatacccc aattcttta
//
LOCUS       DANKHL010000317         1538 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000317 DANKHL010000000
VERSION     DANKHL010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.329"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            35..577
                     /locus_tag="PW101_002836"
     CDS             35..577
                     /locus_tag="PW101_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515202.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            733..1182
                     /locus_tag="PW101_002837"
     CDS             733..1182
                     /locus_tag="PW101_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDL2515203.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1212..>1538)
                     /locus_tag="PW101_002838"
     CDS             complement(1212..>1538)
                     /locus_tag="PW101_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2515204.1"
                     /translation="GKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVE
                     FVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSD
                     TSGEAA"
BASE COUNT          583 a          218 c          302 g          435 t
ORIGIN      
        1 atatttcatc aacgtttcat aaaaggtgtg tagaatgtta ttgaaagtag ctataaaatg
       61 ctattgtgta aaaaataaag gaggaataat ttatgaaaaa atagtaccaa gaggagcatg
      121 cctattactt gcattatgtt tgagcttaag cgcagttggg tgtcaaaaaa gtaatgaaaa
      181 caataaaata aaagaaggac aatccataag ttctgaagag gaaggcatgt caattaatgc
      241 aaatggcaaa aatgaaactt ttaagccatc agattataca ttggaggcaa agaaagaata
      301 tgtttatgaa tatttagggc ttaaatttaa actctcagac aaatttagaa attatatagc
      361 tgataaaaag atagcgatgt tagatgatca aagtcctata gataaagaac taaagtatgc
      421 tattttgaca tttgagaaaa tgacagaaga acaaaagaat gcagttatag aaaagatggg
      481 cgatggatat aaaaattggc aaaatgagct tgaaagaatt gggactattg gtatatttga
      541 aaaaaataca tcagaagaaa aaaatctaaa attataaaat gtgatactca tactaaaata
      601 ggagtttcaa gtgatggaaa atacgattgc tatttaagca caaatagcgg agctgaaagt
      661 aatcttctgg atgaattaaa aagaactgag attcaaatta tagataaaaa agaacgtcct
      721 aaaaatggtt ttgtgctatc agaaaaaact gatttagaaa atacagaagc atttaacaaa
      781 gaatcggtta aagatttacg taaactacca acaaaggaca taaatggcaa ggattttaca
      841 agtaaagatt ttgagaagta tgatcttact atggtaaatg tatttgcaac ttggtgtaca
      901 gcttgtgtaa aggaaattcc tgatttggtt gaggttcaga atgaaatgaa aagcaagggt
      961 gtaaatatcg ttggagttgt tacagatcct gttgatgata acggtgaaaa caaggaagca
     1021 attgaaaagt caaagttaat acatgaaaag acaaaggctt catatccttt cttaatgcca
     1081 gatggaatgg caacactttt tttgacaaaa attataaggt gttgtgaatg ctgcggtgtt
     1141 atggctacga ttgcccttga caatgagcat cctcggatct gattttctcc ggcagaagca
     1201 caatcattca attacgctgc ctctccagag gtatcagatc cgcgctcatt atcaaggatt
     1261 cgcttcgcca atctctgttg tcgaatttgg atcggtgttt catacgccaa cgtgccatgt
     1321 aagcgtagat agttccacca atgcacataa tcaaacaatt ctagacgtag ctgtgccagt
     1381 gtctcaaatt ggtattgatg cacgaattct actttgacag atttataggt tgattccaca
     1441 acggcattat catacggaca acccttccta ctcaatgaac gagtaattcc aaaaccattc
     1501 agaatctcat gaatggtttg gttatcaaat tcctttcc
//
LOCUS       DANKHL010000318         1538 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000318 DANKHL010000000
VERSION     DANKHL010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.330"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            67..1293
                     /locus_tag="PW101_002839"
     CDS             67..1293
                     /locus_tag="PW101_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515205.1"
                     /translation="MIQPTFIRSFTQQADPYDLRKLQEKIMLASVRDESWLVVGNDEN
                     ECSFRLEDNQLLIKNVLSISVFKEKQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTK
                     QISQRLLFEKKDEIEHLPKMKQQNGEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYY
                     HQIIPKPIFLDIQQVEKVKELENEVICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHL
                     AWDNGVGLLKPPFVEYIYTDHTIQSVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKE
                     RRVRGTLNAQAYFPWVDENRSRMMCYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEK
                     YQEYLLSLRIYVPSESLSELPLKEIKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQ
                     FLDYRELEQLITLQKI"
     gene            1304..1513
                     /locus_tag="PW101_002840"
     CDS             1304..1513
                     /locus_tag="PW101_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515206.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT          530 a          260 c          291 g          457 t
ORIGIN      
        1 ctcacagact taaatgaaga agaaaaaaaa caggaaataa gcgattgggc tattttgaca
       61 cgtctgttga tccagccaac atttatccgc tctttcacac aacaagcgga tccgtatgat
      121 cttcgaaagc ttcaagaaaa aataatgcta gcatcggtaa gagacgaaag ttggttagtt
      181 gtaggaaatg acgagaacga atgtagtttt cgcttggaag ataatcaatt attgataaaa
      241 aatgtattgt ctatctctgt tttcaaagaa aagcaatttt tgattcgaga cttcattcag
      301 aaaaaaatga ttgctcatgg ggtgttcgcc tatatgcgag cttatagcga gtttatctat
      361 cacaatacaa aacagatctc tcaacggcta ttgtttgaga aaaaggatga aatcgaacat
      421 ttacctaaaa tgaaacaaca aaacggggaa gtagtagtcg attgcaacca atttccagga
      481 tacgatcttt tttaccaagg tttatgtttc acttcgtgtt gggaaatgta ttatagtcgc
      541 tattatcatc aaatcatccc taaaccgatt tttctcgaca tccaacaagt agaaaaagtg
      601 aaggaattag aaaacgaagt catatgtatc cagctttatc gagacccctt taactggcaa
      661 aagccaaaca atcaaatgtt tcaatcctat tttagggacc agcttgggtt tgatcatctt
      721 gcttgggaca atggtgtcgg tttattgaaa ccgccattcg ttgaatacat ttacactgac
      781 catacgatcc aatctgtcca gtatcagaat gcacaaatgc aacctgttcc taaaaagaat
      841 gcttcatttt ttgtaacaaa aagttatgat attcaacaag gagattataa agaaaggcgt
      901 gttcgcggaa cactaaatgc tcaggcgtat tttccttggg ttgacgagaa tcgctcgcgc
      961 atgatgtgct ataaagttat cgatccgact gttgctttgg ataatggaat cgaagcttat
     1021 tgttattata ttagggaata tctagaagta gaggtaacag atgaaaaata tcaagagtat
     1081 cttttatcct taagaatcta tgttccttca gaatccctgt cagaattacc gttaaaggaa
     1141 atcaaacata gactctctga tgttaccttc aaacgaataa agaaaaagcg gcgaagtatc
     1201 caattcgatg tcaagaaagg tgagcagcat ctgcgtgtgc aatttttaga ttaccgagaa
     1261 ttagaacaat tgattacttt acaaaaaata tgaggtgaag aaaatggata aacaactcta
     1321 ttatcgaata atggttccgg catcattatt atccgtctgt ctcttttttg ttcctaattt
     1381 tcctattcga tcagtcatcg gttttttggt catgtttggc gggatgggac tttattatta
     1441 tcttcttcat aaggaaagaa aacctgcttc tgtttatatg aaaaaaacaa gaagcgaaaa
     1501 taaaaaacga taaaacggct tgatgataga caaaaaaa
//
LOCUS       DANKHL010000319         1505 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000319 DANKHL010000000
VERSION     DANKHL010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.331"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(942..1355)
                     /locus_tag="PW101_002841"
     CDS             complement(942..1355)
                     /locus_tag="PW101_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDL2515207.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          553 a          246 c          223 g          483 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatca
//
LOCUS       DANKHL010000320         1486 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000320 DANKHL010000000
VERSION     DANKHL010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.332"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            45..1412
                     /gene="nhaC"
                     /locus_tag="PW101_002842"
     CDS             45..1412
                     /gene="nhaC"
                     /locus_tag="PW101_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL2515208.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
BASE COUNT          388 a          283 c          327 g          488 t
ORIGIN      
        1 ttttttcatg gcagggcctt ttcatttgaa ataggagatc gacgatgaat aaaaatattt
       61 ctttaaaaga aagtatcgct gtattgatcg tgttgttggc actcctagga gtactgatca
      121 tcggattcca gctctcgcca catatcccga tccttgcagc tttcatgtgt ttgttgtttt
      181 atggcagatt taaaaagttc acatgggatg agatccatga cgggatcatc aaaggaatca
      241 ctccagggat cattccaatc ctgatttttc ttttgattgg tgtcttagtt gcttcatgga
      301 ttttatctgg aaccatccct acgattatgg tttatggatt taaacttgtt tctgtgcgtt
      361 tctttttacc gacagcattt cttgtttgtg caattgtcgg agtgactgtc ggcagttcat
      421 ttacaacgat ttcgacgatt ggaattgctt ttttggggat cggtcattta cttggattca
      481 atgatgcaat gactacaggc gcagtcgttt ccggtgcctt tttagggaat aactgctcgc
      541 ctttatcaga tacgaccaac ttggcagcgg gtattggagg agttaatcta ttcgaacata
      601 tcgctggtat gcgttatacg gatttcccag cattcgtcct ttcgttgatt ttttatacga
      661 tattaggaca taacagtcat tcagccgatc tagctagtat caatgaaatg atccaaaaca
      721 tgaaagcgag cttttggatc tctccttgga cattgattcc actagttatt ttatttgggg
      781 gggcaatcaa aaaaattccg gctatcccta cattactagc gggatcgaca gctgctctcg
      841 ttctttcttt tatccatgag aaaagtttga cggtcggaaa agcagcagac atcttgatga
      901 atggttatgt cgcaaataca cctgatccaa agctagatga actgctttct cgaggcggga
      961 tcatgagtat gttaggttct gctagtttga ttttacttgc attggcatta ggtgggttac
     1021 tgatcaaata tctgatcgtt gataccatcg ttcaagaatt aaaagaaaag atggaccgac
     1081 cttctcgttt agtcgggttt actgccttga gctgtatcgg aatcaatctg atcgttggag
     1141 aacagtatct gtccatcatt cttccaggag aaacattcaa gcgttctttt gatgacgttg
     1201 gcttatcaaa aacctatttg acacgaacac tagcggattc aggtgcgacc gtcaattctc
     1261 tcgtaccgtg gggagtcagc ggcacattca ttatgggaac gatgaaagtt agcgcgttgc
     1321 attacttgcc attcgccttt ttctcgattc ttgcacctgt ctttacaata ttaggcggtt
     1381 ttttattgaa tcgtcgacgt gtaaaaaatt gaaggaagga aaaaagaggc tgggacagaa
     1441 gcgtttaact ccaagaaata agaagaaact caagaaaatt gctttt
//
LOCUS       DANKHL010000321         1475 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000321 DANKHL010000000
VERSION     DANKHL010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.333"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            34..>1475
                     /locus_tag="PW101_002843"
     CDS             34..>1475
                     /locus_tag="PW101_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HDL2515209.1"
                     /translation="MERTARLKKNTIWALVFQITSIVCGMILLRLILTKYGSEVNGMI
                     SSINQFLQIISFLQLGVGAVMQSTLYKPLAEKDNLMISQILASGGKFYRQIAYILLVY
                     VGLLLAVYYVLVPNEFGRSYTMAMIATLSINSFAQYYFGIVNTLLLSADQKIYITYIA
                     QTIAFISNTVACALLISLNTEILMVKLITAIIYLACPIYYRWYVDRNYNIKRNVSYDK
                     EPIPQKWNGVAQHVASVVLDSTDVVVLSIFSNLIYVSIYNVYNLVIYGVKQLFMAMTN
                     GVQALLGELWVRKEIEVLYKTFAWFEWIVHTLTVFIFGCTGILIVPFIQVYTRGVTDA
                     NYNQLSFAALLVTANALHCLRTPYHVMIKAAGKYKETQNSFIIASLLNLGLSLILVKP
                     LGLVGVALGSLISITYHTLWSANYNSKYLLKWPMKKFYKQLFVNILTVVLSFGATSQL
                     KMMSITYLDWIVLAIKCAVIVFICIILVNV"
BASE COUNT          483 a          211 c          256 g          525 t
ORIGIN      
        1 ataaaaagat aaaacaagtc aggagttgca aaaatggaaa gaactgctcg gctcaaaaaa
       61 aacactattt gggctctggt gtttcaaata acttctattg tctgtggtat gatcttgcta
      121 cgtttgatat taacaaagta cggatccgag gttaatggaa tgattagctc tattaaccaa
      181 tttctacaaa ttatttcttt tttacaatta ggagtaggcg ctgtaatgca atcaactcta
      241 tacaaaccac tagcagaaaa ggataattta atgataagtc aaatcttagc ttctggaggg
      301 aagttttatc ggcaaattgc atatattcta ctagtatacg tgggtttgtt acttgcagtc
      361 tattatgttc tcgttcctaa tgaatttggg agatcatata caatggcaat gattgcaaca
      421 ttaagtatta attcatttgc tcaatattat tttggtattg ttaatacttt gttattaagt
      481 gctgaccaga aaatttatat tacgtatatt gcacagacaa tagcatttat ttctaataca
      541 gttgcatgtg ctttattaat tagtctgaac acagagatcc ttatggtaaa acttattact
      601 gctataattt atcttgcttg tccaatatat tatagatggt atgtggatcg aaactataat
      661 ataaagagga atgtgtcata tgataaggaa ccgatccctc aaaaatggaa tggggttgca
      721 caacacgtag cttctgttgt actagatagt actgatgttg tagtgttatc tatattttct
      781 aacctgatat atgtatctat atataatgtg tacaatttag taatatacgg agtaaaacag
      841 ctattcatgg ctatgacaaa tggcgttcag gccttactag gggaactttg ggtcagaaaa
      901 gaaatagaag ttctttacaa aacatttgca tggttcgaat ggattgtaca tactttgaca
      961 gtttttattt ttggctgtac cggtatacta atcgttccat ttattcaagt atatacaaga
     1021 ggagtaacag atgctaatta taatcaatta tcattcgcag ctttacttgt aactgctaac
     1081 gcacttcact gtctgcgtac accttatcat gtaatgataa aagcagctgg taaatataaa
     1141 gaaactcaaa atagttttat aattgcttct ttgttaaatt taggtttatc attaattttg
     1201 gttaaaccac tagggctagt tggagtagca ttaggatctc taatttcaat cacatatcat
     1261 acactttgga gtgcaaatta taattcaaaa tatttgctta aatggccgat gaaaaaattc
     1321 tataaacagt tatttgtaaa catcttaact gtagttcttt cttttggggc gacatcacaa
     1381 ttgaaaatga tgtcaatcac atatttggat tggattgttc tcgcaattaa atgtgcagtt
     1441 attgtattta tatgtataat cctagttaat gtttt
//
LOCUS       DANKHL010000322         1469 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000322 DANKHL010000000
VERSION     DANKHL010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.334"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            131..505
                     /locus_tag="PW101_002844"
     CDS             131..505
                     /locus_tag="PW101_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2515210.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="PW101_002845"
     CDS             complement(619..1248)
                     /locus_tag="PW101_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2515211.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="PW101_002846"
     tRNA            complement(1382..1454)
                     /locus_tag="PW101_002846"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          494 a          225 c          258 g          492 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaataca
//
LOCUS       DANKHL010000323         1465 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000323 DANKHL010000000
VERSION     DANKHL010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.335"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            2..400
                     /locus_tag="PW101_002847"
     CDS             2..400
                     /locus_tag="PW101_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL2515212.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="PW101_002848"
     CDS             384..1079
                     /locus_tag="PW101_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDL2515213.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          553 a          213 c          294 g          405 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattacta actattactt atttctcatt
     1201 taaacgatgt attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt
     1261 gaataaattt cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct
     1321 agtataacag aataagaaga acaagtccag aagggatatt tttagtcttt tttaaaataa
     1381 cttttcagtc gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa
     1441 atagggaagc atgaaagaat ctact
//
LOCUS       DANKHL010000324         1448 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000324 DANKHL010000000
VERSION     DANKHL010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.336"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(1..142)
                     /locus_tag="PW101_002849"
                     /pseudo
     CDS             complement(1..142)
                     /locus_tag="PW101_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(184..1310)
                     /locus_tag="PW101_002850"
     CDS             complement(join(184..1082,1082..1310))
                     /locus_tag="PW101_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2515214.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT          421 a          258 c          237 g          532 t
ORIGIN      
        1 ccagataagt gcgatactca gacgcatcat tcagtaacaa ccgaacggtt gtcgtccccc
       61 aacgaatgat cctagagggg acgacacaaa gacaatggga acactcttct ggcttgtaca
      121 gaagagttcc tttgtaaatc atggatttga gtcaatatct tggacacatt ttagtagaga
      181 cggttaagcc attttttctt ttgctaattc ttcaaattcc tgtggggtta gatagtgaat
      241 cgagctatgg attcgatttc ggttataaaa tccttcaatg tagctaaata gcgctcgatt
      301 tgcttcttca aaatctgagt agctagtcgt gtagacttct tcttttttca atgaggcatg
      361 gaaagattcg attccagcat tatcataagg tgttcctttt ctactataag aatgccttat
      421 tttgttggtt ttaagccatt gttccacttc tcttgctgtg tattggctgc ccaagtcagt
      481 gtgtagaatc attccttctg gaatgtgata atgtattttt gctttattca atgtttgaat
      541 gacacaatcc acagtcattc gttttgaaaa tgtataacta ataatttttt tcgtgtgtaa
      601 atccatgatt gaagataagt aacaccaacc atttttctta gtaggaatgt acgtaatgtc
      661 agctgcccat ttctcacaga tcgtttcagt agaaaagtcc tgatttaaga gattctcttt
      721 tgaaatgatc ggtttattag acctttgagg tctatatttt ttaactacaa tcgaccttaa
      781 atttaattgt ttcattagct tttggacatg tttaagagat actgaaatcc cttcttttaa
      841 taaaacttga tggatctttg tggcaccata gcgttgttta ttcgtaaaaa aaattgttga
      901 aatcttcttt ttcaacttat tatttctgat ttctgtagct gtcaacggtt tattcttata
      961 aaaataatag acacttctag ggatttctaa taaccgacat aacaaagaaa cagtgtaatc
     1021 cttacaccat ttttcaataa attgaacaag cgaatttagg ttttccgaga gaatatggct
     1081 gcggcttttt ttaagatttc aagttcctca tttaaacgag cattttcttt tctcagttca
     1141 gctacttctt ttccagtcag tcctgtggac tgatcaggta aatataaatt cttccatttg
     1201 taaatcgttg cttcagataa gccatattct ttcgctaacc ctcgaacaga acgtccagtt
     1261 tgattcagtt caacaatcat ttgtttaaaa ttttcttcat atcgggtcat aaaaattcat
     1321 cctctcgtag ataagtttaa tttattcttg tctctacgaa aagctgtcta agatttgata
     1381 ctaacaccaa gttatcttta gaaccatttc agatacatat gaaacaatta catatgattt
     1441 tttcataa
//
LOCUS       DANKHL010000325         1432 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000325 DANKHL010000000
VERSION     DANKHL010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.337"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            101..478
                     /locus_tag="PW101_002851"
     CDS             101..478
                     /locus_tag="PW101_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2515215.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            466..804
                     /locus_tag="PW101_002852"
     CDS             466..804
                     /locus_tag="PW101_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2515216.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            938..1381
                     /locus_tag="PW101_002853"
     CDS             938..1381
                     /locus_tag="PW101_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HDL2515217.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
BASE COUNT          526 a          224 c          307 g          375 t
ORIGIN      
        1 ttttaataga aatgagtatt gacttggagt acacttaaag ttgtagaata aataaaatac
       61 ataagtaaag tggcaagaaa ttaagagaaa gagtgatcag atgaatatca aacaagtcag
      121 tgaagaaaag ggcatttcgg ccgatacatt gcgctattat gaacgaatcg ggttgattcc
      181 accagttaat cggacaaaag gcggaatccg tgattataca gaagaagacc taaaatgggt
      241 cgactttacg atctgtatgc gaaatgccgg attatcgatc gagtctttga ttgaatatat
      301 tcgcctatct agcgaaggag aagcaactgt atttgagcgg cgtaagcttt tgattgaaga
      361 aagtgaactt ttgacgaaaa aaattgctga aatgcaagaa tgtttggaac gtttacaagg
      421 aaaaatcaag aaatatgatc gtgtgttatc tagaaataaa atggaatgta tcaagtaaaa
      481 aaatcaagga gcatcgctat ggagttacgc ttactaaaat atttttggac aattgcagaa
      541 gaaggaacaa tctctcaagc cgcagaagtt ctacatctta cacaaccaac cttaagccga
      601 caactgaagg agttagaaga ggaactagga acagaactgt ttgtacgcga aaaccgcaaa
      661 atgatcttga cagaagcagg ttattttcta aaaaagccga gcagaagaaa ttttagattt
      721 aacgaatcaa accactcaag aattcgagaa cagaaaaaaa caattattca gcggccgatt
      781 tacgatcggc tgtgtggaag ctgataattc gaatacattg gcactattac tagaaaagtt
      841 gatccgtgat tatcccagcg ttcaatttca tatccacagc ggaacaagtg atgatatcac
      901 ggagtgacta gaaaaaagcc tgttagattt agcgattttg ctagatccag tagatacaga
      961 gaaatacgaa acgattttgt taccaagaaa ggaaaaatgg ggattgctca ttttagaaaa
     1021 ttctgtttta gcaaaaaaag acgtgataac aaaagaagac tttgcgaatg tacctctttt
     1081 gatctcaaag cgtccagaaa tacagcgaat gtttgctgac tggttaggca taaaaaagga
     1141 gcagttgctg atcacaggaa cgtacaattt gattttcaat gtatttgcgc taattgagaa
     1201 tcaggtaggt gctgctttag tcatagaagg tgctgtaagt catctttcaa agcgagtgac
     1261 attcgttccg cttgacccaa tgattgaaac gaactgcatg ctagtctgga aaaagaatcg
     1321 gattttatca cctgtagtca atgaattgat tcaccgtttc aaagaagctt ttgaacagta
     1381 ataagtatct ctaatcgaag aaatttcaat acaaaagcga gacgtatgac ag
//
LOCUS       DANKHL010000326         1398 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000326 DANKHL010000000
VERSION     DANKHL010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.338"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..698)
                     /locus_tag="PW101_002854"
     CDS             complement(<1..698)
                     /locus_tag="PW101_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL2515218.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKF"
     gene            complement(698..>1398)
                     /locus_tag="PW101_002855"
     CDS             complement(698..>1398)
                     /locus_tag="PW101_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2515219.1"
                     /translation="LLNEYYDEIIEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKK
                     ISYQYHVGQYLPDWHPWEDYRNFFVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMT
                     QKLSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDS
                     LFSWNEETSSMTDIQLYDEVMHDNNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEK
                     DKYIINLINDIEVN"
BASE COUNT          478 a          234 c          151 g          535 t
ORIGIN      
        1 tgaaattttc ttgtaggaac tcttccaaaa taatctaaat gaatttctat aattttatca
       61 tcgtatctca atatatatgt tgctaaatca ttactgttaa tttcaagatt cgaatattta
      121 ccaaactgtt tttttactga tttagggaaa ccaaataggt ttataatata atccaattca
      181 tgtatacagt ctaattctac tcctcccccc atagatttaa aggcagaata attatttcta
      241 taatcagtac cttttctcca atcaggaaga taagtagaac aaatagctct tacagaatat
      301 acttttttac tttgaatact attacgaaca acttccatga tatttttata cctcagtgga
      361 gcagcaacat aaatattttt gtaatcataa ttatattgag taagatcaat actctcagaa
      421 aaagcaggtt tttctaaaaa tatgtaatca actttttcaa caattacatt tagtgtttcc
      481 agatgcttaa aagtcggatt tgtaataaaa gctatatcat aatgtgtatc caaatcgtct
      541 ctattttgaa tatacgttat ttcttcaaac tctttttttc cactgctact ataagctgtt
      601 atatcaaaat taatagaatt cttttttcca tagcttatta gattttttag atgtctcttt
      661 ccaattgacc ctaacccaaa aaaaattact ttcatcatta atttacctca atgtcgttaa
      721 ttaagttaat aatatactta tctttttcaa aagtatattg gggttccatt ttcttttcca
      781 aaagcgtgaa aaattcttta atctcctcta aatatgcatc ttcaataata gtatgtgaat
      841 aattattatc atgcataacc tcatcgtaca attgaatatc tgtcatacta gaagtttctt
      901 cattccatga aaatagagaa tccgggttac cttcccattc tattaaaatc ttttcaccaa
      961 ttatagttaa atttctttta gctactcttg aaacaacatc tacacttaaa gttccaacta
     1021 taccagtttc atgctcaata ataagagtga aactgtccga ataatcaata tcaagattgg
     1081 ataatttctg ggtcattaca gtaaacgatt taactttccc aaatgtatct ataatccaag
     1141 gtaattcaat agcgaatatt tctctacacc cattagtttt tttgtttgaa acaaaaaaat
     1201 tgcgataatc ttcccaagga tgccagtcgg gtaaatactg tcctacatgg tattgataag
     1261 aaattttttt attatgcgtc actttagact taatatattg aatttctttt ctcttcaaaa
     1321 atgtagaaga tagataaagt aatctatctt tctttttagc tacttcgatt atttcatcat
     1381 aatattcatt taataaat
//
LOCUS       DANKHL010000327         1391 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000327 DANKHL010000000
VERSION     DANKHL010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.339"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            33..1372
                     /locus_tag="PW101_002856"
     CDS             join(33..474,474..1372)
                     /locus_tag="PW101_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HDL2515220.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          579 a          180 c          244 g          388 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agttcaactt
     1381 ttggggttca g
//
LOCUS       DANKHL010000328         1383 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000328 DANKHL010000000
VERSION     DANKHL010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.340"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(45..>1383)
                     /locus_tag="PW101_002857"
     CDS             complement(45..>1383)
                     /locus_tag="PW101_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515221.1"
                     /translation="FITFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHR
                     ASLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGT
                     LFLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAP
                     SDMLTHILNNNYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFP
                     WQLFNHTPVKSLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILH
                     LSGVSNSIHANKDSLTLIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQE
                     INPTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELT
                     IPPGINKVVITYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT          526 a          196 c          226 g          435 t
ORIGIN      
        1 gcctgtttat gtgtacagct aaaaagtctt tcttaaataa aaatctagtg agaatgttgt
       61 acttctcttc tcggttgttt ttgtttagaa aaagtaaatc gataaagtaa aaataaaatc
      121 aaagtaacga tagataaata tcttgaagca attgctagtt ttgtgtactg ataagtaata
      181 acgactttat ttatacctgg aggaatagtt aattcagttg tgccaaactt agataatttt
      241 gtttgtacta gtttgccatt aatagaagcc acttgccctt tataacgata aataggtgtc
      301 gtcaagacag tatttttatt attcgaatta tctaattcaa ttgtaagttt gttactcata
      361 aaatgagttg ttggatttat ttcctggtta cctagtaaat attgatcatt attaatcata
      421 tcaggatgat agattgactc tttgtttgca taatcagtat gattgtaatt ttcagctata
      481 gcaacagcgt cttcaggtgc tataagtgtt aatgaatctt tgttagcatg gatagaattg
      541 cttactcctg agagatgcaa tattagtgca agaaacgtaa agaccattat tttccatgaa
      601 ttatttttag tagaaaaaat ataatagatc actatactca tcgtataggc tataaataaa
      661 gaagaaaaac catttaaccg ccagacaaat tgtagactct taactggtgt atggttaaaa
      721 agttgccaag gaaataaatt ggttgaacaa aaaagaacaa atacaccaag aaagaaaata
      781 aataaatcat cagcagttaa tttttttata aaaataaaag caccaatcaa tccgagaaat
      841 aagaataatc caatagtata gttgttatag ttattattta aaatgtgtgt cagcatatca
      901 ctaggagcca taccacttaa ttcttttccc agtgggactt tcaattcttg tgctctagat
      961 tgttgaataa aaggaattaa gaatcccgca cttaaaaaaa aggtgaggac ggtagctttt
     1021 aaaagactta agagtctagc gattttttgg tcccaaaaat aaaaagaaag aaacaacgtt
     1081 cctccaataa atacagaaac catagcgaca gaaagtaaat gagtatacac tactagcgtc
     1141 atgccaatag acaaccagta ccatttttgg taatctctaa tataaatttc gtaacacccc
     1201 attagtatca aaggcaaaaa tgttaaagcg acagcttctc ccaatgaagc acgatggaat
     1261 atgtcaatgg cacgataggc ggaaaatgta taaataatcg aaaataacag cgaaatataa
     1321 cgattgtttt ttatttgttt catagaaaaa taagaaacga ccattgttaa aaaagtgata
     1381 aaa
//
LOCUS       DANKHL010000329         1374 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000329 DANKHL010000000
VERSION     DANKHL010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.341"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            106..1113
                     /locus_tag="PW101_002858"
     CDS             106..1113
                     /locus_tag="PW101_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2515222.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1125..>1374
                     /locus_tag="PW101_002859"
     CDS             1125..>1374
                     /locus_tag="PW101_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HDL2515223.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFI"
BASE COUNT          539 a          171 c          253 g          411 t
ORIGIN      
        1 attcgttacg ttttgacatc attattgata attagtttag ttatatattt tttaaagatt
       61 aagaatcgaa ttatagatga ctataattca taggaggaga tataagtgtt taaaggaaaa
      121 acaatattag taactggcgg aactggttcc attggaagtg aaattgttcg tcagctttta
      181 aaatatgaac caaaagctat cagaatttat agtagaggtg aagaaaaaca attttatatg
      241 caacaagagc tctcaaatca cactaatttg agatttttaa ttggtgatat cagagactct
      301 caaaggattg attatgcatg tcgtgatgtg gatattattt ttcatgctgc cgcaatgaaa
      361 cacgttccag caagtgaata taatcctatg gaagctgtaa aaacaaatgt gatgggaact
      421 caaaatgtaa tagatgcagc gatcaaaaat aatgtaaaac attttgtagg tatcagtaca
      481 gataaagtag tgagtcctac taacaccatg ggtgcgacaa agttattgag tgaaaaatta
      541 ataatagctg ccaatcagta taaaggaaaa acagaaacaa tattttcttg tgtaaggttt
      601 ggaaatgtca tggggtcaag tggatctgtt attcctcttt ttagtaaaca aataaaaaac
      661 aataaagata ttactttgac agatgttgat atgacacgat ttatgatgtc aataccggat
      721 gctgcaagat tagttctgtc tgctgctatg aattcaaaat ttggagaaac atatgtctta
      781 aaaatgccgg caatcaatat tttaacttta gctcaatcat taataaaaat atataatgat
      841 acacatgatg aaaaatatag aggtgaaata aaagttgtag gtatcaggcc aggagaaaaa
      901 ttatatgaag agctaatgac tacagacgaa attgaacgct catatgaaaa tgataaaatg
      961 tatatcattc caccacacta tcatgctact gacgaactgt acaaagatta tgaaaaaatt
     1021 aaaatcactg catactcttc agatgcaaca gaattaatga gtggacagga atttattaga
     1081 atcgcaaaag agaataatgt cgtgacttgg taaggagttt tatgatgaaa tcagaagtat
     1141 atgtaattgc agaaattggt gtaaaccata atggaagtct agatttagct ttagaaagta
     1201 ttgataaagc taaagaatgt ggagctaacg cggttaaatt tcaaacattt aaaacgaata
     1261 agctagtttc caaaaaggct ccaatggcaa aatatcagaa ggataatctt aaagaggaca
     1321 gttctcaatt tgaaatgcta aaaaaattag aattatctca atctgatttt atta
//
LOCUS       DANKHL010000330         1373 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000330 DANKHL010000000
VERSION     DANKHL010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.342"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(218..706)
                     /locus_tag="PW101_002860"
     CDS             complement(218..706)
                     /locus_tag="PW101_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2515224.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(728..1330)
                     /locus_tag="PW101_002861"
     CDS             complement(728..1330)
                     /locus_tag="PW101_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2515225.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT          431 a          319 c          200 g          423 t
ORIGIN      
        1 cagaaagaag cataagcttc tttctgcatt tttacggtac ttgattactc aagcagacaa
       61 gcccttctcc acaaaagtct tgcccgacac ccctgcatcc ggcaggtgat aaagatagaa
      121 atcaattatc ctgtgcctcc aaaatcaatt aggagctctg taaaccagag tccttctcga
      181 cccgtcgcat aaatttactt taacacagtt cctatgttta tgcgagagct tttttcattt
      241 tataatcgac catttgaaat ccttgaccga tatctgaaat aagttcttct gtaatctcaa
      301 atccttcatg cttataaacc tcgattgctt gatgattccc ctgattcact cgcaaaaaga
      361 tctctctttc ctttgtttgc gcagcaagtt cttcatacca gcgaaaaact gaagaagtca
      421 atccttgacc acgtacttta tttagcaaat ataatttact taaataaatt tttccattta
      481 tctcttcata agcagtgtaa ccgacagtct tttcttcgct ttttaataaa aagtaggaca
      541 caccttctgt cagttctttt tgaatctgtt tttttgactg ataagttttc agcatgtaat
      601 ccacttgttt ttgaccaatg atcggcacaa aagcttccgg ccatacttct tgtatgactg
      661 ccatcagctc atccagttca ttttctgtta cttttatttt ttccatacac ccacgtcctt
      721 ttttacatta taaatgacct tgcgcttgat ggaataaaaa gagcatgatc atgctataca
      781 cacccaccga ccatggtttc aagaaaatca caatctgcat aaattcccta aaagataatt
      841 tcgtcaaacc agctactaga cagatcaagt cgtctggcgc aaagggaagc agcaaggtaa
      901 caactagtac ttttttaata tctttcgttt ttcttgtcag caattcatca aatttctgat
      961 acttttttgg cgataaaata agctggacaa aggctacgcc atagtaacgg acaagcaaaa
     1021 atccgattgc ttccccaatc actaggccaa tacaactata taaaagccca aatccattgc
     1081 caaacatcag catgcccgct actgaggaga tccctcccgg caaaatagga atgatcactt
     1141 ggatcacttg caacagaaca aatatcatca ctgcatactt gccaaattgc tggataaacg
     1201 cttgcaaaga cgtgctcgat cgaaaaatcc catgatgata agcataaaga atcaacacaa
     1261 agaaaaaaaa cagaccaata atgggcatga tctgaagaca tttttgtaaa actgatcctt
     1321 ttttagacat ctatattccc cacatctttt ttcatttatt aaaactgcct cct
//
LOCUS       DANKHL010000331         1349 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000331 DANKHL010000000
VERSION     DANKHL010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.343"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(62..280)
                     /locus_tag="PW101_002862"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="PW101_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="PW101_002863"
     CDS             complement(546..740)
                     /locus_tag="PW101_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515226.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(840..926)
                     /locus_tag="PW101_002864"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="PW101_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          482 a          218 c          180 g          469 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttat
//
LOCUS       DANKHL010000332         1339 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000332 DANKHL010000000
VERSION     DANKHL010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.344"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            224..778
                     /locus_tag="PW101_002865"
     CDS             224..778
                     /locus_tag="PW101_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2515227.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            1032..>1339
                     /locus_tag="PW101_002866"
     CDS             1032..>1339
                     /locus_tag="PW101_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515228.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     "
BASE COUNT          544 a          140 c          235 g          420 t
ORIGIN      
        1 gcccgtctaa aatttaaata agagcttaga atcttcatat aagtaaggtt aatttttttg
       61 tacaaaaaag tcggaatatt ctgagttact gaaatgatat aaaatcataa actaaacccc
      121 ttgattttct tcacttttaa cagagtatat atataagttc atgattatgg taaaatatat
      181 atataatcat gaactcagaa tatttttaag aagggaaaca atagtgagct ataatgtaca
      241 accgttaagg acaaatgaag agatacaaga ttttcagttt tggctaagaa gaacaagtaa
      301 tccagaaaga gatagttttt tatttttgtt tggaatcaat aatgggttac gtatgtcaga
      361 tattatagga ttaaaagttg gagacatacg aggtaaaagt aagcctatta ttgttgaaca
      421 taaaacaggt aagcgaaaac cgatttttat agataatttg agagaagaga ttttattata
      481 tactgaaggt aaagaagaaa atgattggtt atttccaagt cggcaacaag gtcgacatat
      541 aacaagggat cgtgtctatc agatatatgc agatatagca gaaaaattag gaagagatga
      601 tataggaaca catacattaa gaaaaacttt tgggtatcac tactacaaga aaactagaga
      661 cattgctacc ttaatgttta tttttaatca tagctcgcaa gcaattacaa aaaggtacat
      721 tggtataact gaagatgaaa taggggcatc gttacgtggt tttaaattgg gaggctaatt
      781 gagatattag gatatatttt ttgtgtataa tgaaaactta gaaggtgaga ttaatgaaga
      841 taactgaatt ttcggaagta tttaaaacgt ttagtaacat ttacaataga ataccagaga
      901 tagaacaaaa tgttgtagtt gataatgatt attatataga gaatgatttt aataaaccaa
      961 gaaaatatga caacctatac aattttatac acaatagaac aacaataaat aacaacagta
     1021 acactcgagt tttgattaat tcagcatatt ttcatattaa atatttaaag aaatatgagt
     1081 atgaagaagt tgtattagat tatgggctaa atagacagtc atttttaatt tatgcaataa
     1141 ataacggaaa tttgccaagc aataaaaaga acataattat tgaaacatat gcctatctgg
     1201 aggggggatc taaaaaacta attaagtcat gtaatataga gtatcaatta tcagtaggag
     1261 aaattaaatg tgttttcgaa gaagatttag aacttgactt aaaaaaaatt tttgccagca
     1321 ttcatgatta tataggttt
//
LOCUS       DANKHL010000333         1336 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000333 DANKHL010000000
VERSION     DANKHL010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.345"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(146..1129)
                     /locus_tag="PW101_002867"
     CDS             complement(146..1129)
                     /locus_tag="PW101_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDL2515229.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
BASE COUNT          377 a          286 c          254 g          419 t
ORIGIN      
        1 atataaacgg tgttcaatca actgaccctc ggtattagct caaaaaacga caaaatatga
       61 agagctattt ttgtttggct gttcttatta cttgatacag gacagctttt tcacgacctc
      121 taattcacgg tgttcaagca agaaatcatt gttcactgaa aagcgttgga tgttcttttt
      181 caaaggtctc taagatctct cgtacttcat cagtagattt cgtattcatc aactgaactc
      241 gcaagtcatt tgctcctgga aatcctttga tatagatttt aaagaaacga tgtagtcctg
      301 tgatggaacg cgggatgatt tctgaataat gatcttgcag atctagttgc agacgaagta
      361 gtccgatcag ttctttttct gtatgttccc tcgggtcttt ttcaaatgca taaggattct
      421 tgaaaatccc tcggccaatc atgatgccat caacgccata tttctcggcc aattcttctc
      481 ctgtctggcg atcggggata tctccattga tcgtgattag tgtttgtgga gcgatttggt
      541 cacgtaaagc aatgatttta ggaatgactt cccaatgtgc atctacttta ctcatctctt
      601 tacgcgtacg caaatggaca gaaagattgg ctatatcttg atgaagtagg tgactgatcc
      661 attcttccat ctcagacatt tccgtatagc cgatacgcgt cttgacgctg acaggcaaac
      721 cgccagcttt agctgcttca atcaattgtg ccgctacttc tggacgaagg atcaagccac
      781 tgccttttcc acgttctgct acattaggga ctgggcagcc catgttgata tcgattcctt
      841 ggaagcccat ttctgccata gcaatgctca tttctctaaa aaattctggc ttgtctcccc
      901 aaatatgggc aaccatcggc tgttcatctt ctgtaaatgt taatcttcct cgtacactgt
      961 cttttccatc tggatgacag aaactgtctg agtttgtaaa ctcagtaaag aagacatccg
     1021 gtgctgctgc atgctttacg acatggcgaa atacaacatc tgttacatct tccattggtg
     1081 ctaaaacaaa aaaaggcttt ggcagctctt tccaaaaatt agtagtcatt tatttgttac
     1141 tctccattct atcaagcagt gaagtcgcaa tctttctcaa aaacattgtc attatactag
     1201 gaaacaaaaa aagaagcaaa gtgaacagct taaaagatgt ttctgattga gcaaaaaaaa
     1261 gactgtgaca aaaatcttat cacagtccat tttccggaat aaatggtgtt caaaagcttg
     1321 catctgcggt aataag
//
LOCUS       DANKHL010000334         1319 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000334 DANKHL010000000
VERSION     DANKHL010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.346"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            24..281
                     /locus_tag="PW101_002868"
     CDS             24..281
                     /locus_tag="PW101_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515230.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            393..764
                     /locus_tag="PW101_002869"
                     /pseudo
     CDS             393..764
                     /locus_tag="PW101_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
     gene            798..>1319
                     /locus_tag="PW101_002870"
     CDS             798..>1319
                     /locus_tag="PW101_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HDL2515231.1"
                     /translation="MPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQMYEKIE
                     SNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLSQKKNV
                     IVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTICVLSTT
                     EFYIQGIDCLNLEN"
BASE COUNT          513 a          145 c          230 g          431 t
ORIGIN      
        1 aaataaaaag gacgtgaaga taaatgtcaa atttagaaaa caaagaagaa aaagttgtaa
       61 acaaaattgt ttctgttgtc aataaactgg ataaagagtt agacgaactt gatacgttat
      121 ctgaaaatcc agaaaaaaaa cataacctaa aaaaatggtt agttgaacga aaggctattc
      181 atgaaattaa aaaagttctt catgaagctg ataaatatga aaaatatgat gaaaaagaac
      241 ttgataaaga atttaaagaa atcaatgact tattactata attgatttat acgactcatt
      301 tttgagtcgt cagactgcaa acaacactct aaatttagga gtttgtctac agtctgaata
      361 gattttaaat ttattgtcca taatgttatt atgaatttat agcattaaga aactatgttg
      421 ttaataaaat atcaccaatt attgattcga caggtctatg tttattagat gaattcttgg
      481 cggatatcag tctatttata atgtttattg atgtaattga agaaacatca agaaattttt
      541 tgctgtctaa gttcagaaaa tttcgtatga aaaatgataa acaaaaatta ttggcaaatg
      601 agatttggag tagtttagaa gtcgaagaaa gtaaacaaga acttttgttg aaatggatac
      661 tttttcttgt aaagaagttc tatgtgaaaa taccagaaac ccattgtctg atagttatgc
      721 acggtaaaat aacagcttct gcgatcgcaa gggaaacaaa taagttgctg aatacatatg
      781 tttatgaggc tttcgatatg ccaattgaag gtgagactag tgacttaatt aacctaatca
      841 acaatttttg taaaagtata gatacgacga atggactcat tctgctagtt gatatgggat
      901 cattggaaca aatgtatgaa aaaattgaat ccaatgtaat aggtgatttg gtaattttaa
      961 acaatgtttc tacagcttta gcgattgaat gtgggattca agtttgccaa aaaaagccaa
     1021 ttagtcattt ttaccagatg gattttgatt cgtttcaagt taaagttcag tattataaag
     1081 gattatcaca aaagaaaaat gtaatagtat cttgtctctc tggtgagggg atttctgaaa
     1141 aagttaagga tattttaaaa cggtatctcg ataatcaagt ggagattttg atttttgatt
     1201 ttaacgcatt aaagaaaata gcaaaggaaa aagatactat gatttttaag aatacaattt
     1261 gtgttctttc aaccactgaa ttttacattc aaggaatcga ttgtttaaat ttagaaaat
//
LOCUS       DANKHL010000335         1286 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000335 DANKHL010000000
VERSION     DANKHL010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.347"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..>1286
                     /locus_tag="PW101_002871"
     CDS             <1..>1286
                     /locus_tag="PW101_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HDL2515232.1"
                     /translation="ENVGNLKNIIQLTCANAFYFLDDLEELYIQMYHLPQWVNFNGMK
                     VMKRQNELKYIQMDDLEKVDEVFNINELIEYLYQTWKKDTDKQQLFEKYDRYFRQKNV
                     DIQNNSALTYPTYQEIRFRQYVKEIERDFQIEFDYYYLQITSIFLNSWIYQIQLTSDD
                     KLYSFINDINSWCSREVQLVNRFIQIGDIYLTKIQKNVLVILFKLMCSKEFQQKRLAL
                     ILAHGDLTATCIASTANRIVERYIYDAIDMPLMTSSSEIADKINRYLQECTGYEEIIL
                     LVDMGSLEKIYLNLELDAQKKFGLLNNVSTRMAIDVANQILLDLPLKQILEQVKENQN
                     VDYIYESSNIKKDMIVCSCASGLGTSFKLKKILEDSFPRDTDLVIETCDYYSLVNENY
                     LSELRKENNILFIVGTLNPHLEDVEFFSIEDMILGM"
BASE COUNT          477 a          160 c          224 g          425 t
ORIGIN      
        1 gaaaatgttg gcaatttgaa aaatatcatt caattgacct gtgccaatgc cttttatttc
       61 ttagatgatt tggaagaact ttatattcag atgtatcatt taccacaatg ggtgaatttc
      121 aacggcatga aggttatgaa aagacagaat gaattaaaat atattcaaat ggatgatttg
      181 gaaaaagtag acgaagtttt taatatcaac gaacttatag agtatcttta tcaaacgtgg
      241 aagaaagaca ctgataagca gcagcttttt gagaaatatg atagatattt tagacaaaag
      301 aatgttgata tccaaaataa ttcagcttta acatatccca cctatcaaga gattcgattt
      361 agacagtatg ttaaggaaat agagagagat tttcaaatag aatttgacta ttattatctt
      421 caaataacgt ctatcttttt gaattcttgg atttatcaaa tccaattaac atcggacgat
      481 aaattatatt ccttcatcaa tgatattaat agttggtgta gtcgagaagt tcaattagtc
      541 aatcgtttta ttcaaatagg ggatatttat ttaacaaaaa ttcaaaaaaa tgttttggtt
      601 attttattta agttaatgtg tagtaaagaa tttcagcaaa aacgtttagc actgattttg
      661 gctcatggag atctaacggc aacctgtata gcaagtactg ctaataggat cgttgagaga
      721 tatatatacg acgccattga tatgccacta atgacttcaa gtagtgagat tgcagacaaa
      781 attaatagat acttacaaga gtgtaccgga tatgaagaaa ttattttact tgttgatatg
      841 ggttcactgg agaaaattta tctaaatttg gaactagacg ctcaaaaaaa attcggacta
      901 ctaaacaatg tttcaacaag aatggctatt gatgttgcaa atcagatatt attagattta
      961 ccgcttaaac agattcttga acaagtaaaa gaaaatcaaa atgtggatta tatttatgaa
     1021 tcatcaaata ttaagaagga tatgattgtc tgttcttgtg caagcggatt aggaacttcg
     1081 tttaaattaa aaaagatact tgaagatagc tttccaaggg atactgatct tgtcatagaa
     1141 acgtgtgact attactcttt agtgaatgaa aactatttat cggagcttcg taaagagaac
     1201 aatattcttt ttattgttgg aacactaaat ccacatctag aagatgtaga attcttttca
     1261 attgaagata tgattttagg aatgaa
//
LOCUS       DANKHL010000336         1276 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000336 DANKHL010000000
VERSION     DANKHL010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.348"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            35..532
                     /locus_tag="PW101_002872"
     CDS             35..532
                     /locus_tag="PW101_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2515233.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(569..1252)
                     /locus_tag="PW101_002873"
     CDS             complement(569..1252)
                     /locus_tag="PW101_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515234.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
BASE COUNT          444 a          216 c          224 g          392 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatca cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt agactggtaa tggaaccata attcgctttc
      601 ctttttgata aatcgctaat tccgtcacac catttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt gattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
     1021 caaattgccc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
     1081 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat tttgatgcct tttaaaacac
     1141 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
     1201 tatcatcgac tttagtaaca ggattggcta agtcaaaatg ttcagttgtc acgacgtaac
     1261 catcgtagtt tgttaa
//
LOCUS       DANKHL010000337         1262 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000337 DANKHL010000000
VERSION     DANKHL010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.349"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..722
                     /locus_tag="PW101_002874"
     CDS             <1..722
                     /locus_tag="PW101_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HDL2515235.1"
                     /translation="FLSTPFDEESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLP
                     VLLSTGMADIHEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVG
                     YSDHSLGYDVGICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEI
                     FLGDGVKRAMPSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQY
                     IGKKVKRPISADSVLLEEDVE"
     gene            719..>1262
                     /gene="neuC"
                     /locus_tag="PW101_002875"
     CDS             719..>1262
                     /gene="neuC"
                     /locus_tag="PW101_002875"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HDL2515236.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILN"
BASE COUNT          418 a          167 c          271 g          406 t
ORIGIN      
        1 attttttgtc tactcctttt gatgaagaaa gtgcagactt tctaaaacaa attggtgtac
       61 aaggatttaa aattggctca ggagatttaa caaatttacc ttttcttaga aaaatagata
      121 tgtatgggtt gccagtttta ttatctacag gtatggctga tattcatgag gtcagtgagg
      181 ctgtagattg tttcatagat agtccagtta ctattttaca ttgtacttca aattatccgg
      241 ctagttctga agatattaat ttattggcga ttaacacatt aaaagaaatg ttcgggatac
      301 ccgttgggta ctccgatcat tcattgggat atgatgttgg tatttgtgct gttgcgatgg
      361 gtgctaaggt aattgagaaa cattttacct tggataagga actacctggt ccagatcata
      421 aagcttcttt agatcctcag gaatttgcag attttgtaaa gcatattaga aacactgaaa
      481 tatttttagg tgatggtgtt aagcgagcaa tgccttctga acttagtaca aaagaagtat
      541 caagaaaaag cctagtggtc actgagaatt taaatgtggg agatattctt acagagaaat
      601 ctcttacaat aaaacgtcct ggaaatggaa tagctcctaa gtttttatat caatatattg
      661 gcaaaaaagt taaaagacca ataagtgcag atagtgttct gcttgaggaa gatgttgaat
      721 gaaaaaaata cttatagtaa gtggaacacg agctgattat ggaatatata aacctgttgc
      781 tgagaattta ctacaagata attgggatgt atcatttgtt gtttctggaa tgcatttatc
      841 ccataaatat ggatttacta aagaattgat cttagctgac ggatttagaa ttagtggaga
      901 agttcaaagt ttgtttttag agaacaataa gggaaacatg gctcggtctg tggcattaga
      961 gataaatggt ttcacaaata tatttgaaca aactagccct gattttgtat tattactagg
     1021 ggatagagga gaaatgttgg cagctgcgat agcttgtttg tatcttggaa tcaaaacagc
     1081 acatattcac ggaggcgaag tgtctggtac tgtagatgaa tcaataagac atgccataac
     1141 gaaaatagca tctgttcatt ttgctgctac ggaaaaaagc aaagagcgtt taataaaaat
     1201 gggggaagat tcttggagag tttttcccgt cggagctcct agaatagata ctattttaaa
     1261 ta
//
LOCUS       DANKHL010000338         1234 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000338 DANKHL010000000
VERSION     DANKHL010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.350"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            169..1179
                     /gene="trpS"
                     /locus_tag="PW101_002876"
     CDS             169..1179
                     /gene="trpS"
                     /locus_tag="PW101_002876"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDL2515237.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT          398 a          231 c          249 g          356 t
ORIGIN      
        1 taaagtttct tagaacgttt attctcccat aaatatttct tccatttcta atctattagt
       61 tattttatcc tctttccttg ttttttcctt aggaactgtt gtttcttttc aggaaaaacg
      121 gtaagatatt aagtgctcag acttacaaat cgtaaagagg tgtctttttt gaaaaatatt
      181 attttaacgg gagatcgacc aactgggaag ctgcatttag ggcattacgt cggttctcta
      241 aaaacccgag tggctatgca ggcagatgaa gacaaccaac tgtttgtcat gattgctgac
      301 atgcaagcac tgacagataa tgctaaaaat cctgagaagg tttcatcaaa tgtcttgcaa
      361 gttgctttag actatttagc cgtgggatta gatcctgcaa aatctacttt atttatccaa
      421 tcacaaattc cggaattagc tgaattaacc atgtattatt tgaacttagt cagcgtagga
      481 cgcgttcggc gcaatccgac tgtcaaaaca gaaatcgaac aaaaaaaatt cggtgaaagc
      541 gtgcctacag gattctttat ctatcctgtt tctcaagcag cagatatcac tgcattcaaa
      601 gcgaatcttg tacctgttgg tgaggatcaa aaaccaatgc tagagcagac acaggaaatc
      661 gtacaaagct tcaatcatac atatggtgaa gtcttggtgg aaccaaaagg cgtattccca
      721 ccaaaaggaa tgggtagatt acctggaatc gatggaaatg gcaaaatgag taaatcgtta
      781 ggaaacggta tttatatctc cgatcctgcc gatgtcttac agaaaaaagt catgagtatg
      841 tatactgatc caaaccacat ccatgtacaa gatcctggac aagtagaagg gaatatggtc
      901 ttcacctact tggatgtatt cggaacagat aaagaagcaa ttgaagaaat gaaagctcat
      961 taccgccgcg gcggacttgg tgatgtaaag atcaaacgat acctcattga tgtattggaa
     1021 gctgaatttg caccgatccg tgcgcgccga gaagaattgg aaaaagatcc ttcagctgtt
     1081 atggaaatct tacgtaaagg tagtgaagaa gctgctaaag tagctgcaca aacactttct
     1141 gaagtaaaat cagcaatggg aatcaattac ttccattaat tcatttactc taaaaaagca
     1201 ggctgtaaag gcctgttttt ttatctcgtc tctg
//
LOCUS       DANKHL010000339         1225 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000339 DANKHL010000000
VERSION     DANKHL010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.351"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            18..428
                     /gene="spx"
                     /locus_tag="PW101_002877"
     CDS             18..428
                     /gene="spx"
                     /locus_tag="PW101_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL2515238.1"
                     /translation="MIKVYTSPSCTSCRKARAWLKANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            442..798
                     /locus_tag="PW101_002878"
     CDS             442..798
                     /locus_tag="PW101_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HDL2515239.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          443 a          176 c          231 g          375 t
ORIGIN      
        1 taaaaaggag tgagaaaatg ataaaagtat atacttcgcc tagctgtact tcctgtagaa
       61 aagcaagagc atggttgaaa gcgaatcaac ttgaatttga agagaagaat atttttgccg
      121 aaccgttaac agaaagtgag atcaaaaaga tcttggcagc gactgaagga ggagttcaag
      181 atattatttc cactcggtcg aaaatttacg aaaagctggg catagatttt aacgaattaa
      241 ctttaaagca aatgatcgtc ttattcaaag aatatccttc attattgaga cgacctattt
      301 tgattgatga aaaaagaata ctaatcggat ttaatgaaga tgagatccgg tcatttgtcc
      361 ctcgtgaaat tcgatgtgtt gccagaaatc tgatctattt acaaaacaat aacatcttaa
      421 attcataggg agggagcctt cgtggaagtg aaagtctatg gatcagcgca ctgtgtaaca
      481 agtctgaagg cgaaacaatg gttagaacac catcaattgc cttatcaatt tgttgactta
      541 gaagaacgcg atttaagtac agaagaagca gaagaattat gcagttttga agaagtgcaa
      601 gcagaacagc tatttgcgac ttggtctgag gcttttcaca acattgcgat tgacctttcc
      661 tgtgtaggaa aaaaacaact gattttttta tgcacacaac ataccaatct cctcagacgt
      721 cctttgatta ttatcgatga agttctattt attggataca atgagcagtt gatggaacag
      781 cttatcttaa ataaatagac tttaactgtt ttaatcaagt cactgagcat ttcatacagt
      841 tgtaacagtc gaacacttag catcaagcgt gttatgtgtt ctttttttga ctttattttt
      901 ctactataga gacatgctca atttatgtgg tcaaaattca aatctaggtg gggatataga
      961 gttaaaaata tattgacatg tgtaaattta catgatatat tcatttctgt tgttttaagt
     1021 agcaaagcaa taaaatagta ttttaaaaaa catataaaat agttgttgac atgttagaaa
     1081 tgacatgata agataactga gttgttaaaa aagaaccact tgtgaagctt gattctatcg
     1141 aaaaaaactt caaaaaagtt cttgacaaat aacaaatgat aagttatgat aaatgagttg
     1201 ctgtaagatt tcgaaaaacg aaatc
//
LOCUS       DANKHL010000340         1225 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000340 DANKHL010000000
VERSION     DANKHL010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.352"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..1154)
                     /locus_tag="PW101_002879"
     CDS             complement(<1..1154)
                     /locus_tag="PW101_002879"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDL2515240.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT          350 a          260 c          224 g          391 t
ORIGIN      
        1 tatattttgt agtaacgaat atcattttcg atctttgtaa tcgaaaaagg tacatctgaa
       61 gtatattctg cacattctcc gtgttcagga agcgtctcac tgaaaatcgt ttcatattcg
      121 tcaacagtga tccgtgttcg agaatctagc atctcttgat gagattgtgc gaagagatat
      181 tcttgatagt tttcttccag ataacctgta aaaaattcac ttactgcacc agaaccgtag
      241 ctgaatagac cgatgcgatc tcctggctgt agtgatttcg aattttccag tagagaagtt
      301 agccccaggt ataatgaacc agtgtaaaga ttaccgattc gtcggctgta acggatgctt
      361 tcttcatagc gagccataag acgttcctga ttttcttcgt ctgtttggtc taatacgctt
      421 tgcaatgcct tttttcccat cttagtatac ggaatatgga aagcaatcgc ctgataatct
      481 tcgagtcctc gacccgacaa ttctttatgt cgattccaaa ctttttggaa tgattcgata
      541 tacgtagaat tagataaagg accatcaaca acaggaaatt cgctataatc tggacgccag
      601 aaatcataga tatcttctgt cagaaatacg ctgtcgtctt caatcgataa aatacgcggg
      661 ttttgagtga tcatcatcgc aacagcaccg acaccttgcg tcacttcacc accgcttgcc
      721 aagctgtaac gagcaatatc acttgctatg actagtactt ttcgttctgg atgatttttg
      781 acatattctt tcgccatgtg caaagctgcg gtgccaccgt agcaagcctc ttttacttca
      841 aaagaacgag caaaagattg gattttcagt aaatggtgga taattaccgc actcgctttt
      901 gaatgatcaa tcccggattc tgttccgacg atgaccatgt cgattagctc acggtctttt
      961 tctgttacga ttttgctggc agcatttgct cctagtgtaa tgatgtcttc atttgcacga
     1021 ttgactgcca tttggtcttg accaatccct atatggtatt ttgcaggatc atccccacgg
     1081 ctttctgcca actctgtcat atctaaatat aaattaggaa taaaaaagga aagacgatca
     1141 atccctattt tcatttcaac acctctttac ttatgataaa aaacttactt tactaaggta
     1201 tcataaaaag attaaaagaa atata
//
LOCUS       DANKHL010000341         1223 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000341 DANKHL010000000
VERSION     DANKHL010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.353"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            42..1061
                     /locus_tag="PW101_002880"
     CDS             42..1061
                     /locus_tag="PW101_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDL2515241.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINNEFNLGPNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMKRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
BASE COUNT          425 a          215 c          247 g          336 t
ORIGIN      
        1 tacaaaatgt tctcggctga aagcgaagaa ggaggaaaca aatgaaaaag aaaattacta
       61 ttactgcgat gagcctgtta acggctcttt ttttattacc cattaatacg tttgcttata
      121 cgatcaacaa tgaatttaat ttgggcccaa acgaaggtag ctctcaagta gcaaataatc
      181 agtacatttt actgcatgag acagctaacg aaacagcaac agggcgcaat gaagcgcagt
      241 atatgaaacg ttcatggact agtgcttaca ctgcttatat tgtgggagac ggcggaattg
      301 tttaccaagt cggacaacct ggttatgtac agtacggtgc tggttcgtat gctaatgcta
      361 acagtcccgt gcagattgag ttacaacaca cacatgataa agcaacgttt gagaaaaact
      421 acaaggcata cgttgaatta gctagagatt cagcaatgaa atatggtatt ccacttacac
      481 tagacacacc gtataatcaa ccagggatca aatcgcattt atgggtaaca caaaatattt
      541 ggggcgatca tacagatcct tacggttatc tttctgaaat gggcgtaagt aaagaaaaat
      601 tagcctatga tttggctcat ggatttaccg atgaaaatcc gacaacttca gatgataaac
      661 cagtcattga tccaactcga gcaggtgctg caaatcctac cctgacagat ggaacaaatt
      721 acgcccacat tgatcagttc ggagaaatcg aaaacgcaaa cttacatgtg gctggttggc
      781 acatcgctaa ctataaatac gagtatatct tcattatgga ttacaatact gggaaagaat
      841 tagctcgagt aaaagctgat ggaatatata gaccagatgt aaatcaagct tataatactt
      901 taggaaatgt tggctatcat gtatctttta acatgcgcaa cttccctaat aagaaagtct
      961 atgtaatgat gcgggcaacg aatgatccag aagggaacac taaaggcgga gcacaagatt
     1021 ttcatgataa acgctggtat ttaaatattc cgcaacgata aaaatagccc ctcatttttg
     1081 aggggaagta catatagttg ttgatatagt tgctaatatt gttagctaat ttgttagtaa
     1141 aactgtatac gaatttagat agtaaaaaaa cagtttgttc cttatataga ggcactttta
     1201 ctactgatta aatgtcagat atg
//
LOCUS       DANKHL010000342         1215 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000342 DANKHL010000000
VERSION     DANKHL010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.354"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            414..947
                     /locus_tag="PW101_002881"
     CDS             414..947
                     /locus_tag="PW101_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HDL2515242.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DANKHL010000343         1212 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000343 DANKHL010000000
VERSION     DANKHL010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.355"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..>1212
                     /locus_tag="PW101_002882"
     rRNA            <1..>1212
                     /locus_tag="PW101_002882"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          318 a          274 c          372 g          248 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc ca
//
LOCUS       DANKHL010000344         1206 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000344 DANKHL010000000
VERSION     DANKHL010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.356"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(34..405)
                     /locus_tag="PW101_002883"
     CDS             complement(34..405)
                     /locus_tag="PW101_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515243.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(407..808)
                     /locus_tag="PW101_002884"
     CDS             complement(407..808)
                     /locus_tag="PW101_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515244.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            complement(822..>1206)
                     /locus_tag="PW101_002885"
     CDS             complement(822..>1206)
                     /locus_tag="PW101_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515245.1"
                     /translation="ISEDVESQQFGTNVINKVINPALSINAPLIPTALSFSVTAVVSE
                     IEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHYA
                     VVKFNGTEIGRQIFDIKVNKPVEKN"
BASE COUNT          381 a          218 c          154 g          453 t
ORIGIN      
        1 aaaaaaataa caaacaaact cgcttgaaat tcattaggtg agtttgttta ttattttttc
       61 tgcttctcga ttcatcagcg ccatatctgt tttcgtaatg gtttcattag gaatatagtc
      121 agcacatttt cttttctcta acattctata cccactatta tgtattccta aggtattcaa
      181 ttccttaact gttgaaaaca aattccaaac ggtttggtgg cttccttttc cgctacttcc
      241 attataaata atatcataat tttctttcaa atactcatca gattttttaa aagcactgta
      301 gtatgctctt gaaacagcag ttcttctata agattcttta gaattatatt tatcatctga
      361 ttcaatttgt ttggaaatag ataaatattc attaaagtca aacattttat tcccccaaag
      421 ttgctactac agatttcccc atatatttat agtcagataa tacattatcg agagtatcca
      481 aaacatcgta atctgaggaa aacaattcag atgttgtttt gataacaaag aaatacaagt
      541 ttaaatcttc atcatatttt atattgattt cagcttttct ttttaaaacc gtatttatca
      601 catcaaaaat tttgccatca ttacttgaaa ttgtgttaat ctcatttttt gtagtaatta
      661 caagttgatt atcattttta gttttcggag tagaacttgc atccacaaca actggagatg
      721 ctagaattac cgatgcgcaa gcaaaaatcg cagcaaaatt tattaagcta ccactccgat
      781 gtgtatttga tggtactata tcaagcatcc gtatcgtctc cttaattttt ttccactggt
      841 ttgttgacct tgatatcaaa gatttgcctt ccaatctcag ttccattaaa tttaacaaca
      901 gcataatgct ctcctgcttc ttcaaccaag acatttctgg cgtttatatt aaagtttata
      961 tcgttaactt gaggaggcaa gcttaccgat acttcacccg tagaaaaaat ttgtttttca
     1021 ttcttgttta aaacttcaat ttctactatt tttatctttt ctgtatcctc tatctcagac
     1081 accacagctg ttacagaaaa tgataaagca gtaggaatta aaggcgcatt aatagaaaga
     1141 gctggattaa ttactttatt aataacatta gtgccaaatt gctgactttc tacatcttct
     1201 gaaatt
//
LOCUS       DANKHL010000345         1195 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000345 DANKHL010000000
VERSION     DANKHL010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.357"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..1076
                     /locus_tag="PW101_002886"
     CDS             <1..1076
                     /locus_tag="PW101_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL2515246.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DANKHL010000346         1191 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000346 DANKHL010000000
VERSION     DANKHL010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.359"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..843
                     /locus_tag="PW101_002887"
     CDS             <1..843
                     /locus_tag="PW101_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2515247.1"
                     /translation="KVYLFFKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRK
                     VVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQT
                     FRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAI
                     DIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNE
                     NSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDL
                     SNLI"
     gene            976..1182
                     /locus_tag="PW101_002888"
     CDS             976..1182
                     /locus_tag="PW101_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDL2515248.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          412 a          183 c          245 g          351 t
ORIGIN      
        1 aaagtatatc tgttcttcaa gcaaggaaaa tcagccttag aatattatca acagtataag
       61 aaaaacaaat caaactgtgg tagacgtccg cttgttttac ccgaggaaca atcagaatat
      121 attcaaagaa aggttgttca aggatggaca cccgatgtga ttgttggtcg tgcagcgttt
      181 cctattagtt gttctgctcg taccatttat cgtatgttca aaaaggggct atttgattct
      241 tctgacttac cgatgaaagg caagcgtaaa ccgaatggac atcaagaaag acgtggaaaa
      301 caaactttcc gccgctctat tcatgaacgt gaaaaggatt atagccaatt ctcaaatgag
      361 tttggtcacc ttgaaggtga cactatcgta ggtctgaaac ataaaagtgc tgtaattacc
      421 ttagttgaac gattatcaaa agttatcatc acattgaaac cgtgtggtag acaagcgatt
      481 gatattgaaa aaaaattaaa tcaatggttt gaatctgtac cgaaaaacct attcaaatcc
      541 atcacttttg attgtggaaa ggaattttca aattggaaac agatcagtaa tgtcaatgat
      601 attgccattt atttcgctga tccaggaacg ccgtctcaaa gaggcctaaa cgagaattct
      661 aacggattgt tacgtagaga tggtttattg aaatctatgg atttcaattc agtagatgaa
      721 ttttttattc aatctgtcgc atctaaacga aataatattc ctagaaaatc actggattat
      781 cgaacacctt tggaagtatt tttgagttac gtaagtattg atgatctgtc taacttaatt
      841 tgacaattaa gaaagataaa attgaacaac tggacgggca aattgatgcc ttgctttcag
      901 aaagagaaat attgatagaa gaaaacaagc gattgaaaaa gaaagtagcc caaccaatga
      961 agggaggtgg atagtatgaa caatagaacc tttgatattt tgaaatggat cgctatagta
     1021 gttatccccg ctttagcgac ttttgtcggt cttgtcggaa aaggtgttaa ctggcggtac
     1081 acagatataa cggtgatgat tattacaggt tttggagcat ttctagggag tattttaggt
     1141 gtatcaaatc gaacgtataa gaaaatttca tcagataaat aggaggatag a
//
LOCUS       DANKHL010000347         1156 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000347 DANKHL010000000
VERSION     DANKHL010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.360"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..1061)
                     /locus_tag="PW101_002889"
     CDS             complement(<1..1061)
                     /locus_tag="PW101_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016372324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2515249.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITE"
BASE COUNT          304 a          257 c          183 g          412 t
ORIGIN      
        1 tcttcggtta ttctttcttc ccaggatttt ggtcttaagg cctcattcag attgataaac
       61 agctttttta ctcgtttctg atactccttc atgacctttt tcattaatgt tttgattcta
      121 cgactataga ttccatagcg acgaatcgtt ttgaaattct tattttgaat atggcgaatc
      181 agtgcagcta tgaattcgtc tatactcatg gtcatcgtct ctttagtatt cgttcgtttg
      241 tccatgtagt tgaacatgac agtgtcgcca tcatacatta agattcggct taatgcgatc
      301 gggccacgct tcatataacg actgatgtat tggagtagtg actttagatt tgtcctgctc
      361 cttttaggag catttacgta aaacccttct ccattgttct tgtacgcagc ttgtaatctt
      421 gattgaactc tttgcttgtc ccactcacct aacgttcgtc taattagttt caatacagca
      481 tttttccagt attttcttaa catagaaaaa ggtaaataat cgtattcttc ccaacttcca
      541 tctcttgtta agccacccat agtaacaacc atatgaacat gcggattaaa ttctagtttt
      601 gatccaaagg tgtggattgt agctactata cctgccttca cctttctttt ctcaaaatag
      661 gcaagaataa tttttgcggc ttcatccatt agtcctttca gtaaggaatc tcgatagttt
      721 tccatcataa agatttcccg taacccctca tcgatcgtaa agattacgtg ccgatgtgtc
      781 actttaaaca tatcattcgc cacaaccgct gaccaccttt ggctttcacc tgttgcacac
      841 gttggacaga actttccacg acatttcaag ggaacaaact ttgtttgatg acatccctcg
      901 cagacaaaca gtcggtatcc tttttgaata tctccacaat atctaaactt gtttacttcc
      961 cgtttgacga ctagccgaat ctttcgcccg tatttctcaa caaagttatc ccaattatgg
     1021 tgttcgtcaa aaaagattgc ttcgagaata ttcgctttca tagtaatatt ttacttgttc
     1081 aaaaacaaaa tgaaaagggc taaaagactg gtagtgcagc ctttttagcc cttaactttt
     1141 tatactttct ttactg
//
LOCUS       DANKHL010000348         1151 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000348 DANKHL010000000
VERSION     DANKHL010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.361"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            36..908
                     /locus_tag="PW101_002890"
     CDS             36..908
                     /locus_tag="PW101_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2515250.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          334 a          220 c          280 g          317 t
ORIGIN      
        1 agtgatttca ttctaaaata aagggagaaa tgtctatgct atacggtgca attgaagcag
       61 gcggcacaaa atttgtgtgt gccattggcg atgaagaaat gacgatcaag gaaagagtga
      121 gctttccgac aacgacacct gaggaaacaa tgcctttagt cattgacttc ttcaaacaat
      181 accaagcaga tttagcaggg attgggattg gctcatttgg gcctatcgat atccatcgcg
      241 attccgccac ttatgggtac attacctcga ccccgaaatt ggcatggcaa aattttgatt
      301 tcatcggtac catgaaaaaa gaatttccga tcccgatttc ttggacaaca gatgtcaatg
      361 cggctgccta tggtgagtat gtcttcggca gtggcaaagg attgagcagt gtcgtttact
      421 atacgatcgg tacgggtgtt ggcggcggtg ccttacaaga agggcgcttt atcgaagggt
      481 ttagtcatcc agaaatgggg catatgctcg tcgtccctca tccaaaagat gactttgctg
      541 gcagctgtcc atttcatggc aactgtttag aagggatggc cgctggaccc gccatcgaaa
      601 accgcttagg gaaaaaagga caagaggttg cagaagatga tccttattgg gaaatcgaag
      661 cctcctatat tgctcaatgt gcctacaata caaccttgat gttttctcca gatgtgatca
      721 tttttggcgg tggtgtgatg aaacaacgcc atatgctgca aaacgtccac gacgcatttg
      781 caaaattggt caatgggtat gtcgaaacac ctgagttaag caaatatatc gtgaccccag
      841 cattagaaga caacgctgga acgctcggtt gtttggcatt ggctcgagaa gcggtcttac
      901 attcataaac gaaaaaaggc tcttcgacat ttagtgttgg tagagaaaga tacaactgaa
      961 aatttagtca taaccagtta ggcagagtgc ttaactggtt tttgttttac ttggaagatc
     1021 aagccttgaa tgagataaat tcttgctcga aagttatgga aatttcgata gccgtaggcc
     1081 acttgtattg tgcaatagtt ttttacaaaa aaaagggggt gcggatattt ttttggacct
     1141 ttttaacaaa t
//
LOCUS       DANKHL010000349         1142 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000349 DANKHL010000000
VERSION     DANKHL010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.362"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(389..634)
                     /locus_tag="PW101_002891"
     CDS             complement(389..634)
                     /locus_tag="PW101_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2515251.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(628..1029)
                     /locus_tag="PW101_002892"
     CDS             complement(628..1029)
                     /locus_tag="PW101_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2515252.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
BASE COUNT          364 a          206 c          150 g          422 t
ORIGIN      
        1 agattaatca aatcttttcg agtagctgtt ttcattatag caccttcctt actttttttc
       61 atttatttct atcagctgct tttgtcaaaa gcacatagga atttcacctc tccccaagtt
      121 tacggcgagc cttacggaag tatcattatc ccaactttaa atcatcgctt tttattgact
      181 ataaaaatta ttttagaata tttatcgctc cttctttaca gcaagaaatc atggcgcaac
      241 taaaatatgc tccttaaagt gttaacgtac tgatagaatc tatttaattt tcaatgaaca
      301 ataggttttc tacctaaaag aatctactaa ttccttaact attagaagat tcttttaaac
      361 agaaaaagtg gaaaggggat ttttttaatt agttaatttt aaattttaag atcgcttcta
      421 ttaatttaat ttctagctga cttatcatat attcatctac ataggtaacg aattgaccta
      481 cctcgttaaa tccatttgtc aaacataact tagccatata agaatgataa tgtttgagta
      541 tcatcttgat cgaaattgga tctccttcta gagcagataa gataatggat agtggaacta
      601 ataggtactg ccgcttttta gatttatcta ccataggtca taccccattt tcaattgttc
      661 aactgccttc tttcgttgat accagatacc acttgtgctc atattgagaa ttttaccgat
      721 ttcagcatca ttaaaacctg caaaataata aagtaggata atctttcttc ttatctccga
      781 taactgatca attgctgctg ataacttttg atctgaaatt aatatttcca ttccaaataa
      841 gaaatagaca tcttgttcat caaaatgagt ttgggaatac ctgccttcat ttgaatctgc
      901 caatggttcc tcattatcct ttatgttagc gtttcgtttt cgaatagact tagcttcgtt
      961 tcgtaatact tttttacagt aggcatcaaa ttggtgttgt atagatatgt cattgtaatg
     1021 ttgttgcatg ataatatgct cctttttccc cctttccact aatacaacaa tcagaaagcc
     1081 cctttttttc gaaaaaacta gaaaaaaaac ataaaaatat ttatcattct ttaaacagaa
     1141 aa
//
LOCUS       DANKHL010000350         1053 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000350 DANKHL010000000
VERSION     DANKHL010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.364"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(208..912)
                     /locus_tag="PW101_002893"
     CDS             complement(208..912)
                     /locus_tag="PW101_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515253.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT          304 a          189 c          191 g          369 t
ORIGIN      
        1 atcttaagaa atcaggcaac tccaagatat cttcaggtag aagagaagtc gcctaacatt
       61 gtaggatcat aggtgatttg ttggacaggt caagctgata tttgagcctg atgttagaag
      121 gaatttcttt ttacttgctg tagaaattat tatatgacta aaaaggctgg gattttaaat
      181 cccagccttt tatgacaaat agaattttta tcgatcagta atcgctgtcg ctgcaaattg
      241 ttcgatcaaa ggaatcaaag tcaatttgac tttgtatttc cgttcgagtg ctttttcgat
      301 aacgaactca ttttcagtag tcatagaaat agttgctaat ttacgaggtt catcagcatt
      361 tttttcatta ataactactt catgttcatt ttgttctgtg taaaatttag gatactcagc
      421 aacggctttt gaaaaatcag caaaacttaa agcggcattc gtatcagttg agcgtgtatt
      481 ttcagttgtg tccgcttttg gtttttccgt tttttcttct ggcacactga ctgtttcttc
      541 tgtaactggt gtcgcaacag cttgcgcagt tggttcatcg tttttcaatc gttgatcatt
      601 ccagtaagac acgatttcaa taagaagatt gtcacggaat cgttcgaaaa tcccgttttc
      661 tcctgaacga attctcttta atacagtatt gaagttgtca tcgattggca cgttgatccg
      721 tgtatcttca tttgttttcc cagctaatcc gatcgttaag atttgacggt ttttgggatc
      781 aagcatgact tgtaacggca aaccataacg tcgcaagctc tcttcttcta gagaacggaa
      841 taattgtcct cttacaccat ccaatgaagg acggaattga tttaataatt tttgtttctg
      901 ttttttcttc atatgttctc tccttcgctc ataccctttc gattataaga aaaaaacagc
      961 caaatgaaaa ggagtaatgc aaaaaaactt gcgtatacgc aaattacaac agaaaaattt
     1021 gcgtattttc ccgttttttg aaagaaaaat aaa
//
LOCUS       DANKHL010000351         1038 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000351 DANKHL010000000
VERSION     DANKHL010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.365"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            100..840
                     /locus_tag="PW101_002894"
     CDS             100..840
                     /locus_tag="PW101_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515254.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT          381 a          185 c          200 g          272 t
ORIGIN      
        1 tcatcgctat tttcttaaaa taatcagcgc attgctactg aaaacaatga atctgtgcta
       61 ttattgagaa aagttccatt tttgagaagg atggtttgaa tgaaagaaat cagacgaaaa
      121 catttccgtt ttcctgcgta cgatgatgag ttaggcgtaa aattaaccaa cgacaagaag
      181 cgagtattat ttgacggaca agaagatctt ttgactgaac agaatcagtc cccgtttgaa
      241 cagatgaaag aatttcattt cacgaacacg aacaccgaga aaatccgtcg caagcgtcgt
      301 gcaacaaatg gattgaagtc agctccgcgt aaggaaaatt tggaaaagca taaaggaaaa
      361 ttaccggatt ataccggcca ttaccaatca gaaatcacat cgacaggacg aaaaaaactt
      421 tttggtgatt cctctcgttc tgtcagacaa agtgaaacaa ggacagagtc caagcatact
      481 acacgaaacg catctgttga cacgaaggtg cgggagcgtt cttattttgt tcctaaatat
      541 gtacccgcat cgattatccc agaccctaga gaaagcaaaa tttctgagga tcatttaatc
      601 gaatcgatga aaaaaacgga tgactcttat ctgttgttcg atacagaacc gacgccgtat
      661 caagaaaaaa gaaatggtga gccgtctgta cgaacattca atcgaacaaa aacgttagag
      721 gaaacaaagc agtcagaaga aaaaaaatcg aaaaaaagcc atggtatctt agaacgctca
      781 ttacaaggat tgatcgatga tcaaggaaac tctttgggag aaaatagcta ttttaaataa
      841 gtgatataca aatttatatt cggctacagt tggaaaggaa acgctgcggc gtttccttat
      901 tttatactct atccgaaagt ttatatcgta tcctcaaatt tttttgagtt taagcgaatt
      961 ttttctccca ataagtaaga aaataatggc aaaataaaaa aacaaactat tttcgcagat
     1021 cgtttgcaaa aatagttt
//
LOCUS       DANKHL010000352         1022 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000352 DANKHL010000000
VERSION     DANKHL010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.367"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(22..837)
                     /gene="thiD"
                     /locus_tag="PW101_002895"
     CDS             complement(22..837)
                     /gene="thiD"
                     /locus_tag="PW101_002895"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDL2515255.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          267 a          224 c          159 g          372 t
ORIGIN      
        1 ctttttcaca acttctttct tttactctaa ttcaccagct gcccaatgat tgatggggcc
       61 atatttgtgc cctacattta tttcttcacg aattgcacgg tttacatagt cttttgccaa
      121 tcggatcgct tgttcaactg tttttccttt cgctaactca gctgcaatac atgcagatag
      181 cgaatcgcca gttccgttga tccggtcggt aggataaaat ggtgcattta accaaaatga
      241 ttttcctgat tcgaacaata cgtagtcatt gactgtttct tgctgcgcat ttttagcatg
      301 atgtcctttg atcataacgt tttttgcacc catatcttgc agttcttttg ctgcttgcac
      361 aaaatcttct tcagctgcta atgtccgtcc agtcagcttt tctgcttcat aaaaattagg
      421 tgtcagtact tcggccaaag ggacaagctt ttctttcaag ctttgcaaag cagattcttc
      481 tagtaaaaga tttccatgtt tcgttacgat tacgggatcc acgactaatg gtccaaaatc
      541 atatttttgg taatttttta caactgtctc aatcaacttt gaatctgcta gcatacctgt
      601 tttagctgca cgtatttgaa agtccttcgc aatcagttca aattctttgt cgataaaatc
      661 tgttggcaat gtcacacttg ctccgattcc gtaagaatta cctgcaacac atgcggtcat
      721 gacagaaaca ccgtaaacat ttcttgtaaa gaatgtatgc atatctgctt gcatccctgc
      781 gcttccatcg ctatctgatc ctgcgatcgt cattgcttgt acatactgat cttccatcaa
      841 atcgattcct ccatttttat aaaaagattt ttcataatac tgttactgtt tctatcatat
      901 gtttttcaaa gtatccctca gtaaatatac cactaccttt tcttggtaat caaatttatt
      961 tgaaaaaaca tttgcaataa atcagaaaat agtggtcgtt tatttgtatt cgctattcta
     1021 at
//
LOCUS       DANKHL010000353         1000 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000353 DANKHL010000000
VERSION     DANKHL010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.368"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            369..1000
                     /locus_tag="PW101_002896"
                     /pseudo
     CDS             369..1000
                     /locus_tag="PW101_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac
//
LOCUS       DANKHL010000354          998 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000354 DANKHL010000000
VERSION     DANKHL010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.369"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..302
                     /locus_tag="PW101_002897"
     CDS             <1..302
                     /locus_tag="PW101_002897"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515256.1"
                     /translation="NIAAFLGVGILSLFFQFKKRSIGSQREKWSQYFDKIGQKGLVTR
                     LHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKYQLNKNHLNRTFR"
     gene            complement(419..745)
                     /locus_tag="PW101_002898"
     CDS             complement(419..745)
                     /locus_tag="PW101_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515257.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
BASE COUNT          287 a          217 c          164 g          330 t
ORIGIN      
        1 tgaatatcgc tgcttttctg ggagtcggta ttttatccct tttctttcaa ttcaaaaaac
       61 gaagcatagg gagtcaacgg gaaaaatggt cgcagtattt tgacaaaatc gggcaaaaag
      121 ggctggttac acgcttgcac attagttaca tggttgcatt atgcttatta gcgacaatca
      181 attactattc ttttttcgat cattcaatcg cttatacgat tacgctactg attgctggga
      241 tttttcactt atcttataaa tatcagctga ataaaaacca cttgaatcgg actttccgct
      301 aaaaaatatc cgtacgataa gtagagtgcc ttgaagatcg gcaaaaaact gccggacttt
      361 ttagaggctg tgacaaagat tttgtcacag cctctttttc catataaacg gcgttcgcct
      421 accagctgcc tggtaaatct gtttcctctt cgttaagaag cgaaaatccg tcaaatgttt
      481 cgccatcata ataacgaagc agctcatcca tggacaagtt catcgttttt tctttttctt
      541 cttccgctga ttcagccagt agctgttccc gatacacctg atacatacca gctatttttt
      601 gctgcatcgc ttttctagtt tcaccttccg cattgattat tggcaattct ttgatatagc
      661 cacgataaat caaccgatca tctgaaaatt caatgggtaa ttcaacgagt gtcaacccga
      721 cttcctgaaa atattcttcc agcatctatt atccccccat tctttttctt aaatgaattt
      781 tagcttacct actgaaaatt ttccagtttc attattttga gaaaaaaagg gaatgtgaca
      841 aaaatcgtgt cacatcccct ttttccgaat aaatggtgtt cttctgccaa ctcctcaatc
      901 ataagtcaca atattcaaca aacatgagaa actgtttttt gaaattgttc cttgtgattc
      961 agagttaaac gacagtctca caacctcttg gtaaacgg
//
LOCUS       DANKHL010000355          991 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000355 DANKHL010000000
VERSION     DANKHL010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.370"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(21..94)
                     /locus_tag="PW101_002899"
     tRNA            complement(21..94)
                     /locus_tag="PW101_002899"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="PW101_002900"
     tRNA            complement(108..181)
                     /locus_tag="PW101_002900"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="PW101_002901"
     tRNA            complement(209..281)
                     /locus_tag="PW101_002901"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="PW101_002902"
     tRNA            complement(301..374)
                     /locus_tag="PW101_002902"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
     gene            complement(384..457)
                     /locus_tag="PW101_002903"
     tRNA            complement(384..457)
                     /locus_tag="PW101_002903"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Arg,seq:acg)
     gene            complement(468..553)
                     /locus_tag="PW101_002904"
     tRNA            complement(468..553)
                     /locus_tag="PW101_002904"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Leu,seq:taa)
     gene            complement(570..641)
                     /locus_tag="PW101_002905"
     tRNA            complement(570..641)
                     /locus_tag="PW101_002905"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(607..609),aa:Gly,seq:gcc)
     gene            complement(653..725)
                     /locus_tag="PW101_002906"
     tRNA            complement(653..725)
                     /locus_tag="PW101_002906"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(690..692),aa:Thr,seq:tgt)
     gene            complement(743..824)
                     /locus_tag="PW101_002907"
     tRNA            complement(743..824)
                     /locus_tag="PW101_002907"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Leu,seq:tag)
     gene            complement(832..904)
                     /locus_tag="PW101_002908"
     tRNA            complement(832..904)
                     /locus_tag="PW101_002908"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(869..871),aa:Lys,seq:ttt)
     gene            complement(908..980)
                     /locus_tag="PW101_002909"
     tRNA            complement(908..980)
                     /locus_tag="PW101_002909"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Val,seq:tac)
BASE COUNT          260 a          283 c          235 g          213 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat taacgcgccc aagaggagtc gaacccctaa ccttttgatc
      421 cgtagtcaaa cactctatcc agttgagcta tgggcgcata tttgaaatgc cgaggaccgg
      481 aatcgaaccg gtacggtgat cactcaccgc aggattttaa gtcctgtgcg tctgccagtt
      541 ccgccacccc ggcgcgattg ctttggcaaa gcggaaaacg gggttcgaac ccgcgacccc
      601 caccttggca aggtggtgct ctaccactga gctatttccg cgtgtttaaa gatgccggct
      661 aaaggacttg aaccctcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      721 ccggcatgta aaacttaaat tatgcgggtg aagggacttg aacccccacg ccgtaaggcg
      781 ctagatccta aatctagtgc gtctgccaat tccgccacac ccgcaaaaat atgagccgta
      841 cagggctcga acctgtgacc ctctgattaa aagtcagatg ctctaccaac tgagctaacg
      901 gctcatatgg aggttaacgg gatcgaaccg ctgaccctct gcttgtaagg cagatgctct
      961 cccagctgag ctaaacctcc atggataact t
//
LOCUS       DANKHL010000356          977 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000356 DANKHL010000000
VERSION     DANKHL010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.371"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..906)
                     /locus_tag="PW101_002910"
     CDS             complement(<1..906)
                     /locus_tag="PW101_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL2515258.1"
                     /translation="MVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPGLLARQGF
                     IEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSS
                     SRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAK
                     DFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVI
                     RPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAF
                     ICIYAFYVLNIFSLLKTFFRSKVNYA"
BASE COUNT          372 a          163 c          121 g          321 t
ORIGIN      
        1 cgcataattc actttacttc taaaaaatgt tttgagtaaa ctaaagatat ttaacacata
       61 gaaagcatag atacatataa aagcaattat accacttgat acaagtactt gaagaaatcc
      121 attatgcatg tttccatgtg ctctttttag ttcattcaca tctgtagtat caagatctac
      181 ttttttcaaa aattttgcat cgctattccc atagaaatcg gtctctccat aaccaaataa
      241 caaagttggt cttataactt taagcgcagc tttccaaatc gtaaaacgac cagctgaaat
      301 ttcggagtta tcactagaat gctgtaatat tacttctttt tctggaagtg gagttttatc
      361 tgattcattt gactctataa cttctacttt tctatcaatc gatacaggaa ttgttgctaa
      421 aaaatcctta gcataattag taccaaaaga aaaatatacc aatagaacta ataatatgct
      481 taccatccat ttgattttta caggtagtac ttctctaatg ctctttctaa attttgaatt
      541 ggcaaacaac aaaagaaata atactaaaaa agaacaaatt actacctggg cacctctaga
      601 cgaagaaagt gtaatataaa atatttgtac gataaaattc atcatataaa aaacacgaat
      661 cgtttttgtt atttttgaaa ttaatacact aattaaactt aaaataatag aagtatatcc
      721 aaacatagct ccaacatttg gacttgtata gacaccaaat aaccgatgtt caataaaacc
      781 ttgtcttgca agtaatcctg gcgttcctgt tgggacatga taagaaatca aaaaaataaa
      841 ttgatagata gatattagcg cagctaaaaa aattatgata atgaaaacat tattaaaata
      901 caccattttt ttcttttttt cctcttcact ttgtgaaaag tctgcaggaa aaattactat
      961 caaagtaatc agcaaat
//
LOCUS       DANKHL010000357          959 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000357 DANKHL010000000
VERSION     DANKHL010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.372"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(107..511)
                     /locus_tag="PW101_002911"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="PW101_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="PW101_002912"
     CDS             complement(570..>959)
                     /locus_tag="PW101_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2515259.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DANKHL010000358          954 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000358 DANKHL010000000
VERSION     DANKHL010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.373"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(38..952)
                     /locus_tag="PW101_002913"
     CDS             complement(38..952)
                     /locus_tag="PW101_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515260.1"
                     /translation="MLKDLKQYYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLF
                     FSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESF
                     ENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEY
                     INDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKN
                     LSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILL
                     TIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          308 a          162 c          145 g          339 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attt
//
LOCUS       DANKHL010000359          950 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000359 DANKHL010000000
VERSION     DANKHL010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.374"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            796..>950
                     /locus_tag="PW101_002914"
     CDS             796..>950
                     /locus_tag="PW101_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2515261.1"
                     /translation="MEEVCNMSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHE
                     QLDQLPP"
BASE COUNT          345 a          158 c          120 g          327 t
ORIGIN      
        1 ataagtagtt ctatctgagc gatactcttt tataatactg aattcggaat taaaaaccac
       61 gataggtaac tttaaacaaa catgaagaga ctttaataag acttctttat ctgaaaacac
      121 ttttgtttct ctctttcctt tctacatcat taaatagtat tttatacgtt atctaaaata
      181 ctattcctct gtcaaaataa gctaacttac ttcattataa aatatccact tatcgctttc
      241 atgtatattc caccgtaaaa agtacatttt atcaccgaga actcatcatt tttcatcatg
      301 atgaatacta tttacatata tctagttcaa agacaataat tattatgatc cgttattatc
      361 taagcactat acagataata ggaatttttc gtttctaaat actccaattt gggaatccaa
      421 taaaaatttt cgttcaaata cgtgtctatt cgaactcttt tctagttgtt aattatgaag
      481 ggggaagaaa taaaaaatac aatctataca cgaataagaa tattatccaa aaggaggttt
      541 tagaaaagat ttattatacg taaaaagctc aagacatttt cttagccata aggacatgtt
      601 tgatatttaa gaagatatat gaattttctt ttctaagtat aaagataact atccattatc
      661 aactaaaaaa acatttttga aaataaacat cagcagtcct atcaatgcat gtgagcttat
      721 agaagttatt cgcagcgcag cataattgca ttagcttctt ttcgtgtttt ttgcctcttt
      781 tccctttaaa tcttaatgga ggaggtgtgc aatatgtcag ataacgacga acgcgtaaat
      841 gcccagttga ttcgattctt tgaaaaggtc attttgaaca ccgctaatac ttattataaa
      901 aaacaagaag aaattcaaaa acatgaacaa cttgatcaac tcccaccata
//
LOCUS       DANKHL010000360          948 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000360 DANKHL010000000
VERSION     DANKHL010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.375"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..517
                     /locus_tag="PW101_002915"
     CDS             <1..517
                     /locus_tag="PW101_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2515262.1"
                     /translation="EFNLSFGDNKIIFAEDNWFQLQVFMTNTRICFVDKLCYYYRQQN
                     NSIMHKPKSNLVFRHGNMIADYEKLIRQNGNNIIDKKACMLVAFDVLIMEIFNQKNVG
                     GTCEDYKNALVSLKHHKILRENISNIVRMKAYELEPNMRKKLALVTISIMFNFKMYKT
                     AYNMLWRIYNR"
     gene            531..>948
                     /locus_tag="PW101_002916"
     CDS             531..>948
                     /locus_tag="PW101_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2515263.1"
                     /translation="MRKLTFVIGIMGKGGAERVIANLANNFIQDNWEIDIITIYGNRQ
                     DYELNRKIQVHPIICKSSFRAFRPLERFKELRKKIREIQPDLVISFLADVNIHVIISL
                     LGVKQKLIVSERNDPQRNPEEPWIRKIRDFLYKNVDG"
BASE COUNT          375 a           96 c          179 g          298 t
ORIGIN      
        1 agagtttaat ttatcatttg gtgataacaa aataattttt gcagaagata attggtttca
       61 attacaagta tttatgacaa atacaagaat ctgtttcgta gataaattgt gttattatta
      121 tcgccaacaa aataattcta taatgcataa gccaaaaagt aatttggttt ttagacatgg
      181 aaatatgatt gctgattatg aaaagttgat aaggcaaaat ggaaacaaca taatagataa
      241 aaaagcttgt atgttggttg catttgatgt actaattatg gagattttta atcaaaaaaa
      301 cgttggtgga acatgtgaag attataagaa tgcattagtt tctttgaaac atcataaaat
      361 attgagagaa aatatttcaa atatagttag aatgaaagca tatgaacttg aacctaatat
      421 gagaaagaag ttagcccttg tgaccattag tataatgttt aattttaaaa tgtataaaac
      481 agcgtataat atgctctgga gaatatataa tcgataaaga ggtaggaaat atgaggaaat
      541 tgacctttgt gataggaatt atgggaaaag gaggggcaga acgagtaatt gcgaatttgg
      601 caaataattt tattcaagat aactgggaaa ttgatattat aactatatat gggaatcggc
      661 aagactatga actgaataga aaaatacagg tacatccaat aatatgtaaa agttcgtttc
      721 gtgcatttag acctttggaa cgctttaagg aacttaggaa aaaaataagg gaaatacagc
      781 ctgatctggt tatttcgttc cttgctgatg taaatatcca tgtcattatt tcattgttag
      841 gagtaaaaca aaaattaatt gtttctgaac gaaatgatcc acaacggaat ccagaagaac
      901 catggattag gaaaattaga gattttcttt ataaaaatgt agatggaa
//
LOCUS       DANKHL010000361          943 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000361 DANKHL010000000
VERSION     DANKHL010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.376"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..>943
                     /locus_tag="PW101_002917"
     rRNA            <1..>943
                     /locus_tag="PW101_002917"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DANKHL010000362          929 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000362 DANKHL010000000
VERSION     DANKHL010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.378"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            244..552
                     /locus_tag="PW101_002918"
     CDS             244..552
                     /locus_tag="PW101_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDL2515264.1"
                     /translation="MFQNKGQRYVTKGVMDSLLVELQTLCWNLIDQNVQKKLPLDYLQ
                     IFEFSIEKGNQKLVHRQEEPEARKEYLISPKLRLESVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            579..878
                     /locus_tag="PW101_002919"
     CDS             579..878
                     /locus_tag="PW101_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HDL2515265.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGTLPKNVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVCGLLVEGRLVWSQIGGTNE"
BASE COUNT          337 a          123 c          189 g          280 t
ORIGIN      
        1 agttactaat aactatataa attttaaact tgtagtcgca atttagtata gaggtactaa
       61 atatgaaaac tccatacttt tagaaaagca tttcttatat ttagttgtga tgtctattca
      121 gttaattgaa ataaataata tagcaatgaa atagagataa attcaaatat atattatatc
      181 catgaagtcc aatcttcttt atagaagatt gggctatttt ttacgacaaa ggagggaaaa
      241 gaaatgtttc aaaataaagg acaacgttat gtcacaaagg gagtaatgga cagtttactg
      301 gtggaattac aaactttatg ttggaattta atcgatcaaa acgttcaaaa gaagcttcct
      361 ttagactatc ttcaaatctt tgaattttcg atagaaaaag gaaatcaaaa gttggttcat
      421 cgtcaagaag aaccggaagc aagaaaagag tatctgattt cacctaagct tcgtttggaa
      481 tcagtgtctc aaaaaatatg ggtcattgat tcgggtgagt atcaaacgat gttattacca
      541 gaagaatact gatatctaaa agaaaggagc gaaatgactt gttagaaacg gctatcgaag
      601 tactggaaaa gtgtgcacag ctagtcacta ctcctgaaga gtggggctat gaatccgtta
      661 cgatggaaaa agaggaaatt gaaatgggta cgcttcctaa aaatgttcat ctaccacgtt
      721 tagtgatgac acatctttat atttattgcg caccagaaga tggaaaagac tatgtagttt
      781 atttcattac agatattacc agccagcgag aatttgtttg tgggttactg gtagaaggac
      841 gattggtttg gagtcagatt ggaggaacga atgaataaga tcgattattt gagaatgatt
      901 gcgaaatgtt tagaagatat ccttaccgc
//
LOCUS       DANKHL010000363          920 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.379, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000363 DANKHL010000000
VERSION     DANKHL010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.379"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(56..>920)
                     /locus_tag="PW101_002920"
     CDS             complement(56..>920)
                     /locus_tag="PW101_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL2515266.1"
                     /translation="GKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPEL
                     EEWQARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTE
                     SKNRWMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRY
                     LPSKDYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFD
                     YGSAIRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFM
                     KTNARSDSYDV"
BASE COUNT          285 a          194 c          163 g          278 t
ORIGIN      
        1 aactagatca tcaatcgtaa gcatcgcaaa ttgatttcta ccattcgtat tgtttttaga
       61 catcatagct atcactcctt gcattcgttt tcataaatct attataaaag aaaagactgt
      121 agacatcatc cataaaatgg actttgtcta cagtctgcgc attctcatta gagaacactc
      181 ccttttttgt gacttttctg aagctagagt gaatactttc aactgcatta gtagtgtaca
      241 tgatttttct aatggcgctg ccataatcaa agagttgttc aacatgagaa aagtttcgtt
      301 tgcatacatc tactgcacca gaataatgag accaccgatt ttgaaaactg tcgaaggcag
      361 cgtgtgcagc gtttaaagaa gaagcactgt agaatttttt catgtctcga cagacttcct
      421 tataatcctt acttgggagg tatcgtaacg catttcgaac aagatgaaca atacagcgct
      481 gcacaatgac agaaggaaaa attgcttttg cgccttcttt gagaccggaa acaccgtcca
      541 tcgaaatgaa aaaaacatct tctacaccac gtactttcag ttcatcaaat acttgcatcc
      601 agcgattttt agattctgtt tgattgagcc ataggcctaa aatctccttg tttcctttaa
      661 gatcatagcc aagaatggtg tacacagcat attctttagc ttcatagttt tcgcgtaaag
      721 tgacgtacat acaatcaacg aatagaaatg cataacactt ggctaatggc cgagcttgcc
      781 attcttccaa ttcaggaagg acggcatccg tgatatcaga aatcatttcg tgggaaatat
      841 caaagccgta aatatcttca acggttgcgg cgatatcgca ttgactcatt cctcgtgcat
      901 acatggaaag aaccttacct
//
LOCUS       DANKHL010000364          910 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000364 DANKHL010000000
VERSION     DANKHL010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.380"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            4..>910
                     /gene="bsh"
                     /locus_tag="PW101_002921"
     CDS             4..>910
                     /gene="bsh"
                     /locus_tag="PW101_002921"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDL2515267.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKE"
BASE COUNT          312 a          122 c          167 g          309 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagttact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgacgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg taggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag
//
LOCUS       DANKHL010000365          908 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.381, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000365 DANKHL010000000
VERSION     DANKHL010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.381"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            82..>908
                     /locus_tag="PW101_002922"
     CDS             82..>908
                     /locus_tag="PW101_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HDL2515268.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQK"
BASE COUNT          303 a          168 c          180 g          257 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaag
//
LOCUS       DANKHL010000366          906 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.382, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000366 DANKHL010000000
VERSION     DANKHL010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.382"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..720
                     /locus_tag="PW101_002923"
     CDS             <1..720
                     /locus_tag="PW101_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2515269.1"
                     /translation="GKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFT
                     NRLGTNKRGRLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVY
                     LANCYTLEGRKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITL
                     EEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNK
                     VQLIRLNKYRVLAGNQELNLI"
BASE COUNT          339 a          143 c          178 g          246 t
ORIGIN      
        1 gggaaaacgg aattagaaga aacaagactt tataattcaa tggtattagg tatgcaaaat
       61 tattatcaga ttgcaacgaa tgtaaatctg gattgtatga aactgaatag agctgtaatg
      121 gctatcttca ctaacagatt aggtacaaac aaaaggggca gattgagaaa aacaggcaga
      181 caacttacga caaaggaggc aacaagatat ggtaaatcta aaatgcttcg gtatgttgct
      241 ggcgagggcg aacctatttt ccctgttgga tatatccaac acagaaaacc aatggcaaag
      301 gtgtatttag cgaattgcta cactctagag ggaagaaaat ttatccacac caatctaagt
      361 gttgataaat tactgatgta ccagttaatg aaattatctt tggaaaatcg cacaatagaa
      421 tacgcagaca atcgaatttc gctattttct gctcaacatg gaaaatgtgc tataactctg
      481 gaagaatttc aacaagctaa tgaaatacat tgtcatcata ttgtaccaac tggagctggt
      541 ggaaatgacg attataaaaa tcttattttg gtaaaagagg cggtacaccg tctaatacac
      601 gctaaaacag aagaaacaat acaaaaatat atagctctat tgaagctaaa taaagtccag
      661 ctcataagac taaataaata tagagtatta gctggcaatc aagaattaaa cttgatatag
      721 gtctactaca taaatacttt tcaatataaa cacgatggaa cgcggagtgc ggtgaaagtc
      781 gcacgctccg tgtgaatggg gggaaaagtt tgagatattt ataatatcag gaacttacct
      841 atccatatat caatggtttt taaccaatgc tcaatcttta gtcttactag cgattgtggt
      901 cattgg
//
LOCUS       DANKHL010000367          905 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.383, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000367 DANKHL010000000
VERSION     DANKHL010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.383"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            1..905
                     /locus_tag="PW101_002924"
                     /pseudo
     CDS             1..905
                     /locus_tag="PW101_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          330 a          157 c          172 g          246 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag cgatcgtaac
      481 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
      541 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      601 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      661 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      721 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      781 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      841 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      901 gacac
//
LOCUS       DANKHL010000368          892 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.384, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000368 DANKHL010000000
VERSION     DANKHL010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.384"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..558
                     /locus_tag="PW101_002925"
     CDS             <1..558
                     /locus_tag="PW101_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515270.1"
                     /translation="KNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSD
                     GYGIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFS
                     STLLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYN
                     RLSEEKILLPENWAELRKLVEYGII"
     gene            545..>892
                     /locus_tag="PW101_002926"
     CDS             545..>892
                     /locus_tag="PW101_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDL2515271.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFD"
BASE COUNT          315 a          119 c          130 g          328 t
ORIGIN      
        1 aaaaatataa aaaaaattcc caaaatagat gaaaacagta aattgatgaa aaacatacaa
       61 tcaatctttg ggatagaaga agtttttgtc ggagataaat gtatcttttt tgatcaacct
      121 ttattatctg atggttatgg catagatgaa acagatattt tcgctaaagt aaatagtata
      181 ttttctggaa aaaaagaaac tttaattaag cttcatccta gagcaaggac aaatcgttat
      241 ggtgaaaaaa acatattgaa ttccagttta ccgtttgaag tcttgctact tgaaaatgac
      301 ttctcttcaa cacttttgat tagtcctata tctacaattt ctttttcacc atctatgatg
      361 tttgaaaaga cgatcaactc tattatgttg gctaaattaa ttttaaatga atatacagca
      421 ttatctgaag ataaaaaaga aacattgaca aatattgttc ttttctgtga acattataac
      481 cgtctatcag aagaaaaaat tttactccca gaaaactggg cagaattaag aaagttggta
      541 gagtatggga ttatttaaca cactttccaa gtcaaaatta gttaagtcca gtttctggta
      601 tactataggg aattttttta ttaagggaat ttcattcata actattccta tttttgtaaa
      661 tattactact caagcagaat atggtttgat taataatttc acttcatttg tatcaatttt
      721 ttcgattctt gttgggctaa gcctaaattc ggcaattaat aatgcaagtt ttgactataa
      781 agatgacata aatgaattta tgtcatcggt actttctcta gctacacttt cgttaattgt
      841 gttcttatta atagggaaca tatatttttt gttttcaaat gctttttttg at
//
LOCUS       DANKHL010000369          880 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.385, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000369 DANKHL010000000
VERSION     DANKHL010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.385"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..875)
                     /locus_tag="PW101_002927"
     CDS             complement(<1..875)
                     /locus_tag="PW101_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2515272.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTG"
BASE COUNT          299 a          105 c          156 g          320 t
ORIGIN      
        1 aaccctgtat attttttaaa agctgaagaa aaatgatgga tcgaatgaaa tcctaatata
       61 tctgcaattt ctgtataggt taattcattt tctagcacga gacgttttgc tttttccatt
      121 tttagttctt taaaatagct aataacactt tttccattca cttttctaaa atgttttttt
      181 aagctggctg gactgataaa aaaaatttgg gctaattgtt caagcaaaaa attttctgaa
      241 agatgttctt ctaaatatcg tatgattgga tcaactaatt catctgaata aagaatatgc
      301 ttttttttag gaatgacttt ctcatttgaa ggtattgtca ataaagcaag ttcctgttga
      361 taatgccttg gtaaggaaga caattgaatt ttttgcagac aataagtaac taataaggaa
      421 agcaaatgaa tgctcatctg ttggcatagt atgaaaaaag aattgatttc ctgatcatag
      481 gaaatagctg gcttactatg ttttagataa tcaattttga actgttcatt tttcagaaaa
      541 gtaatgatag atgttatttc ttgatctata gaaaaagatt gtcccgtaat atgtgctaat
      601 aattgctcat caaaataaaa ctggataatg ataaaagttt gtgtttcctt ttgagtagaa
      661 aagatttgga cttcttgctg tttatcaatg aataaaaggg aaggattatc tagttctata
      721 gatttatgtg caaattcagc atataaatta gcgctttcat ttattagtag tcgaaaatag
      781 ggagcagcag ataaatgaag atgggtatct atggaatagg ttagtgtttt tttgacaaga
      841 ttggaggatt ggttgtttgt taacaaaaga accactttcc
//
LOCUS       DANKHL010000370          846 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.386, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000370 DANKHL010000000
VERSION     DANKHL010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.386"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(37..237)
                     /locus_tag="PW101_002928"
     CDS             complement(37..237)
                     /locus_tag="PW101_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2515273.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            633..782
                     /locus_tag="PW101_002929"
     CDS             633..782
                     /locus_tag="PW101_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515274.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT          272 a          144 c          134 g          296 t
ORIGIN      
        1 ttttctttcg aaaagacggt taaaagtcaa tttttattaa gctttaacaa tgtttgttgc
       61 ttgagggcca cgttgaccat cttcaacatc gtaagacact gcttggcctt cttctaaagt
      121 tttgaagccg tcaccttgga tagctgaaaa atgtgcaaat acatcatttc catcttcacc
      181 agtgataaag ccaaaacctt tgtctgcgtt gaaccatttt actgtaccgt tattcataaa
      241 taaattcctc ctgatacgta tcaatgtacg ttttttcgtt gcaataaata tttgataggc
      301 aaaaggagtt ttatctatgt agatatatcg ctcaaattac gtttcaaaat agattactca
      361 tttaatatat catgcttttt ctaataacac aaggataatg caatgaaata taaaattcat
      421 tgtcttctat tgtcttcgaa tcgaagaaaa agttgaataa ccgcgattga ttcacttttt
      481 gttttcttgt ttatttgctt ttatctgcaa cttttgactt taaaatttct taactatgaa
      541 aacctttcaa ttggtggtat acttataaat ggaatttcgt tatcaacgcg ttcgttgcct
      601 ttcagcaagt atttaaagaa aggagaatgg ctatgacatt ctataacaaa gatatacaat
      661 atcgagtaac cttggataca gatttgaatc tatttgtcgt ttttgacaaa gaaaatacca
      721 atcatgtagc gacaggtgtt acgatcgaac aagcagtgca agaattaaaa aaatctgcat
      781 aaggagtgga aaccctcata tggacgattc tcgttcttct ctttaaacgc atctactggt
      841 agataa
//
LOCUS       DANKHL010000371          822 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.387, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000371 DANKHL010000000
VERSION     DANKHL010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.387"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            10..>822
                     /locus_tag="PW101_002930"
     CDS             10..>822
                     /locus_tag="PW101_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515275.1"
                     /translation="MEIFGFYDTAFHINRALSLESIFSSPINFETFRSYGMQVNNFYP
                     WLTLYPLFLLIKFTNLAIGYNLFLYIVTLITLFICHYVMYEITKKHVTSSFFAIIYTT
                     SSFRSVEIFLRGAMGELLAMSILPLILLGFIKLYDSKKESWVMLAISMTLLIYTHVLS
                     VAMTMVMIIIALIIQFYRKKKIEIFLIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLN
                     RPYVDILQKRAIYLGKEFLIGSIDSELLSFGIGLTLLICLIILTFNFKKLGIL"
BASE COUNT          261 a          113 c          119 g          329 t
ORIGIN      
        1 tttaaaaaca tggaaatatt tggcttttat gatacggcat ttcacattaa tcgtgcgtta
       61 agtttagaat ctatttttag ctcccctatt aattttgaaa cgtttagatc atatggcatg
      121 caagtaaata atttttaccc atggttaact ctatatcctt tatttttatt gatcaagttt
      181 actaatttag caataggcta taaccttttt ctttatattg ttacgctcat tactctattt
      241 atatgtcatt atgtaatgta tgaaattacc aaaaaacatg ttactagctc ctttttcgct
      301 attatatata caacatcttc ttttaggagt gtagaaatat ttcttcgagg ggcaatgggg
      361 gaattactcg caatgtcaat acttcctctt attttactcg ggtttataaa gctttacgac
      421 tcaaaaaaag aatcttgggt tatgttggcg attagcatga ctttattaat ttacactcat
      481 gttctttctg tagctatgac tatggtaatg attataattg ctttaattat tcaattttat
      541 cggaaaaaga aaatagaaat cttcttaatt attaaattaa taaaagctgc tatagttact
      601 ttacttcttt cactagcgtt tataggtcct atgattgagc agacattgta tcaagatctg
      661 aatagacctt atgtggatat attacaaaaa agagcaattt atctgggtaa agagtttttg
      721 attggaagta tagattcaga attactatca tttggtattg gcttaacttt gcttatttgt
      781 ttaattatac taacatttaa ctttaaaaaa ttaggaatct ta
//
LOCUS       DANKHL010000372          819 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.388, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000372 DANKHL010000000
VERSION     DANKHL010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.388"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(496..720)
                     /locus_tag="PW101_002931"
     CDS             complement(496..720)
                     /locus_tag="PW101_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515276.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT          296 a          113 c          146 g          264 t
ORIGIN      
        1 acttcttttt taatttgttc ttgcaatctt ttttgctacg taatttttgt aaaaaagtgg
       61 gttgagagaa atctaattga ttttcaaaga aggagtatga aaatgagaaa aatatttatt
      121 cgtttattgt gggtactatc ttagaggaat aaataatagt ggagaaaact atggtttggc
      181 gcagattcta tcaggtttga tggaacgaag acaacctccc gacatcaagt caaaagccac
      241 ttgagatttt gatctgaaaa taaaaaaaga aaacaaggta ggaacatcaa tgtttctacc
      301 ttgttttcgt aatcaatggg aaaaaactaa gagtggtgta cgtaatcaat aaaaaggatg
      361 gtgacaacag tacctcttta ctctctgaaa gcatcataac tcgtttttcc tcattatgcc
      421 aataaattgt tgttatacca catataagga tttgtaatag ctaaaaacag acaggaaagt
      481 tgaaatctgt gaatcttatt ttgtatggaa ggatttatag aacgcaaaaa tcaagataaa
      541 gatgaataaa aatcctgttt cggctatata ttgcgtatct acgccaataa aacgagtttg
      601 ttctgtcata gccatataaa gcaaaggcaa aaatacaact acagcgagat aagatccgat
      661 gatttctcga aaagttttcc aggaagtatg tttcctgttt ttatatttat ccgatttcat
      721 tgaatagtca tagcctcctt attctttcat atgttgaaat tccagtataa aaactggaag
      781 gaactgtacg ttttttagaa taaaaattat aagggataa
//
LOCUS       DANKHL010000373          790 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.389, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000373 DANKHL010000000
VERSION     DANKHL010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.389"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            23..715
                     /locus_tag="PW101_002932"
     CDS             23..715
                     /locus_tag="PW101_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDL2515277.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          198 t
ORIGIN      
        1 gaaaaaaagg attttagtaa caattggtct agcttttttg ttagctggtt gtaccaacgg
       61 gaatgaatcg aaaaaagaat caacaaaaga aacagcgaaa gcaacagttg caactactac
      121 aacaactagc acagagagca agtcaactgc agcaagtaca gaaacagcag atacctctac
      181 ttcgtcagag tcttccaaaa cgactgaaac aactcaaaca tctacttcaa gttcttctgt
      241 agaggcttca tcttcctctt cagaaagcag cgaacaagtt gaacaaacac tgtggaatgc
      301 agacaaatcc gcacaattag aggcatttat gaccaaatgg ggccaaacaa tgggacaaca
      361 atacaaaagt tatacgaatc aaatgagtgt ggacttatat ggtcttaaag tccctcaagc
      421 tattttgaac ggggagtgga agatggctat tggtggagtg cctgtttctg cagaatggtc
      481 agaaagtggg acaggtcaag cagactatca aatcacagcc gtctattccg atgcagagac
      541 agaaccatat ctgaaaaagc acgtctatct attcgggttt cagcaaaatc agccaaaagt
      601 attggtgaca caacaaaatc aggggaatcc cgataattat ctttacttca acgagacagc
      661 gaataatgaa ttgaaaaatg gctttaatca aatcgtttat ggacaagcaa attaatgaga
      721 taaacaaggg gttgtgacat gtcttttatc acaacccctt gctcagttta tttgttagtc
      781 ttttattttt
//
LOCUS       DANKHL010000374          770 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.390, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000374 DANKHL010000000
VERSION     DANKHL010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.390"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            116..482
                     /gene="ssrA"
                     /locus_tag="PW101_002933"
     tmRNA           116..482
                     /gene="ssrA"
                     /locus_tag="PW101_002933"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          250 a          126 c          150 g          244 t
ORIGIN      
        1 aaaggggcag ttattatata aataatggga tatgattcgc ttttttattg aatttatccc
       61 aatatatgct atactaattt tgtatgaaaa atacacgtaa cccttgttag ctaacgggga
      121 cgttacggat tcgacaggca cagtcgagct tgaattgcgt ttcgtaggtt acgtctacgt
      181 aaaaacgtta cagttaaata taactgctaa aaacgaaaac aactcttacg ctttagctgc
      241 ctaaaaacag ttagcgtaga tcctctcggc atcgcccatg tgctcgagta agggtcctaa
      301 ctttagtggg atacgtttca actttccgtc tgtaagttga aaaagagaac atcagactag
      361 cgatacagaa tgcctgtcac tcggcaagct gtaaagtgaa tccttaaatg agttgactat
      421 gaacgtagat ttttaagtgg cgatgtgttt ggacgcgggt tcgactcccg ccgtctccat
      481 atatgtatgg ttgagacaga taaacatgta tgaaaaacgt tgttaaatca acgttttttt
      541 attttggtca aggagagaaa agtagagaaa tacaaaattt tttgcattga ttttgcattc
      601 aaaaaaacat tcaaacattt agaaatcccc aatacgtatt caggaaaatc tattttaagg
      661 tttacaaaaa gaacttttgt tcgtacactc tttttgaggt gaacttttat gctgatggaa
      721 ggaaaaacac aattatggtt taagcatgtt ccttcaaata agttttttta
//
LOCUS       DANKHL010000375          764 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.392, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000375 DANKHL010000000
VERSION     DANKHL010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.392"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..690
                     /locus_tag="PW101_002934"
     CDS             <1..690
                     /locus_tag="PW101_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2515278.1"
                     /translation="NPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTA
                     GNKKGIVVVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTV
                     TLPNGSQYHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSE
                     KAVSKDNKTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGL
                     LGYKNRKKENE"
BASE COUNT          307 a          107 c          156 g          194 t
ORIGIN      
        1 aatcctgaag gacaaaaagt gactacagac ttaaataaag agccagacgc atctgaggga
       61 attaagaaca aagaagatct accaaaagga actacttata cttggaaaga gaaagtagat
      121 gttagtacag ctggaaacaa aaaagggatt gtagtagtaa catatccaga tggatctaaa
      181 gaagaagtag aggttactat ttctgtagaa gataaaaaag caccgaataa acctcaagtt
      241 gatcctatta cagagggtga ccaaattgtt actggtaaaa ctgaaccaaa tgcagaggtg
      301 acagttacat taccaaacgg gagtcaatac catgggacag ctgataaaaa tggtaatttt
      361 acagttaaag ttcctaaatt agaggcagga acaaaagtga tagtaactgc aactgatgaa
      421 tctggtaata ctagtgaacc tactaatgta gttgtttctt caaacgaaaa agacagtgaa
      481 aaagccgtta gtaaagataa taagactgac aatcaaggta gcaagcaaaa caaaaataga
      541 ggcaaaagca gtcctcaaaa gcagtcctca aaagcttatc ctaagacggg agagattgat
      601 agtaatatct ttactattag tggtggttta atcctgttag gaactttagg gttattaggg
      661 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaaatct tctctgaaaa
      721 gaggaagaat ctttttcttc ctctttttgt tttttatttt ggga
//
LOCUS       DANKHL010000376          763 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.394, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000376 DANKHL010000000
VERSION     DANKHL010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.394"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(156..455)
                     /locus_tag="PW101_002935"
     CDS             complement(156..455)
                     /locus_tag="PW101_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2515279.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          248 a          133 c           96 g          286 t
ORIGIN      
        1 gtttttgtgt taattaaagg ataaaataca atataaagaa acatctgttt ctttatttag
       61 aataaaaaaa tacagactct gttcaaataa atgaacctag cctgcattat gagattgact
      121 atacttttta attcattgag atgaataact cgtttttata aatgcccaaa agtaatcggt
      181 gccgaagcca ttcctcgacc taattctttt tcaaattttc ctattgttga tatctccata
      241 taaaacggtg atactggttt agacaattct cccctgattg ctaacggacg taaactacgt
      301 tccaaattat ttatgacttt aggataatac tgaccttttt ctaagcctgt ctttgcgtct
      361 atcaaaagta tacgttcctt ttctttgatc tctggattta aaacgagatc atataatttt
      421 agtaccatgt tttctatttc ttcactttta gacatcacga tttcctccca aaaatatatt
      481 tcttcccact acattctata aacaaagtat acgctacatc tagatttttt taaatcatta
      541 ctggattgtt aaaacctttc aatacactgt tatggcaacg atttttattt taaaaacatt
      601 ctaaattaaa tcagtaaaaa tttttattct aagattttta ttagaattac gcctaatttg
      661 tgtctctcag ggcaattcta agaacgtttc aagagatagc gacactatcg ttatcgaaaa
      721 aggcgaaaac ctatctcatt ttctgctaat gtaagttggt tta
//
LOCUS       DANKHL010000377          736 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.395, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000377 DANKHL010000000
VERSION     DANKHL010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.395"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..696
                     /locus_tag="PW101_002936"
     CDS             <1..696
                     /locus_tag="PW101_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDL2515280.1"
                     /translation="KDVASVLGYSNTKDALSRHVDLEDKMGSRITTSGQSREMTIINE
                     SGLYSLILKSKLPSAKKFKRWVTSEVLPAIRKHGGYLTPEKVEEALLNPDTIIQLATQ
                     LKEERTGRLIAEQKIAEYEPKISYLDSILSSTDSVTISQIAADYGMSPQQMNKLLHKL
                     GIQKKVGNQWLLCKKHMNQGYTKSHTTEIPKADGGTKIVMNTKWTQKGRLFIYELLKK
                     EGYYPQMDLEEIG"
BASE COUNT          285 a          123 c          142 g          186 t
ORIGIN      
        1 aaagacgttg cgagtgtttt gggttattca aacactaaag atgctttgtc gcggcatgta
       61 gatttggaag ataagatggg gtcgcgaatc acgacctcag gtcaatcaag agagatgaca
      121 atcatcaacg aatctggttt gtacagttta atcttaaaat caaaacttcc ctctgccaaa
      181 aaatttaaac gttgggtaac aagtgaagtg ttgccagcaa ttagaaaaca tggaggttat
      241 ctaactccag aaaaagtaga agaagctttg cttaatccag atacaatcat tcaattagca
      301 actcaactaa aagaagaaag aactggaaga ttaatcgcag aacaaaagat tgcagagtac
      361 gaacctaaaa tctcctattt agatagcata ttatcttcta cagattcagt aacaattagt
      421 cagattgcag cagattatgg gatgtctcca caacagatga ataaattact tcataaacta
      481 ggtattcaga aaaaagtcgg taaccaatgg ttattgtgca aaaaacacat gaaccaagga
      541 tacacaaaat ctcatacaac tgagatcccg aaagccgatg gtggcactaa aattgtaatg
      601 aataccaaat ggacacagaa agggcgtcta tttatctacg aattactaaa aaaagaagga
      661 tattaccctc aaatggattt agaggaaatt ggttagaaag gagttttagt atgactgaca
      721 ttgcagaaat cactca
//
LOCUS       DANKHL010000378          708 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.396, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000378 DANKHL010000000
VERSION     DANKHL010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.396"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..>708
                     /locus_tag="PW101_002937"
     CDS             <1..>708
                     /locus_tag="PW101_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDL2515281.1"
                     /translation="ILSFISTFLNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLV
                     IYLRIMIKGAHFFDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIY
                     SYISNIGIILSVIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVV
                     SIDLAKFMAPKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAI
                     VNILLNMIFIPRLGIIA"
BASE COUNT          219 a           88 c          132 g          269 t
ORIGIN      
        1 tgattttatc ttttatatct acttttttga atattgcaat ctctgtagct ttgatgcaga
       61 cattatattt tgatgataaa tatgttggca gagtagtcgg cggaacactg tcatttgtgt
      121 tgataggttt agtaatttat ttaagaataa tgataaaggg tgctcatttt tttgataaaa
      181 aacagtggag atacgcttta aaaatctctt tgccgctaat cccacattcc ttagcaaata
      241 tagttttagc acaattcgat agaattatga ttaattctta tttaggtcct gctgcggggg
      301 gaatatattc ctacattagt aacataggta ttattctaag cgtaatatgg atgtctacta
      361 ataacgcctg ggttccatgg ttctatggtg agatgagtaa aaataatgaa aaaaaagtga
      421 agaaagttag taactattat ttagtagtct ttactttgat tacgatgatt gtaatggtag
      481 tttcgattga tttagcaaaa tttatggctc cgaaagacta ttattctggg ttatctttag
      541 tggttccgat aaccttaggc tatttctttc agtttttata tagtttacca gttaatgctg
      601 aattttatga gaaaaaaaca tcgtttattg cgattggtac agtagcaagt gctatagtta
      661 atatactatt aaacatgatt ttcataccta ggttaggaat aattgctg
//
LOCUS       DANKHL010000379          695 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.397, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000379 DANKHL010000000
VERSION     DANKHL010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.397"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            50..>695
                     /locus_tag="PW101_002938"
     CDS             50..>695
                     /locus_tag="PW101_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2515282.1"
                     /translation="MKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRTPI
                     REALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQKK
                     RILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERFRW
                     LRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHP"
BASE COUNT          279 a          107 c          114 g          195 t
ORIGIN      
        1 gaattaaaat atcagttaaa ataaaagtta aggagcattg tgtatgacaa ttaaaactag
       61 tctccaaaat caagcttacc aatctattcg ccgacaaatt atctatgctg acttagaacc
      121 tggaaaaaaa gtatcagaaa aaaaattaga agaaacactt tctattggtc gtacaccaat
      181 acgtgaagca ctgattcaac tccgacaaca aggtcttgtt gatacaattc ctcaaagcgg
      241 aaattatatc tcattaattg atttagattc ggccaaaaat gctcgatttg tcagagaaca
      301 tttggaacga caaattatgt tagagtgttg tgccaaaatg aataaccaaa aaaaacggat
      361 tttagaaact ataatttcag aacaagaaaa agctgttagt caaaaaaatg ctcgtagttt
      421 ttttcataat gataatttat tccataaagc ttgttttgaa attgcagagc gagaagaagt
      481 atggcaatgg ttagaaaata acaatacaca cttagaacga tttcgatggc tacgagtaaa
      541 aacggaagga ttaaagtggg aaaaaattat ggaacaacat tatcaactat ttgaggcatt
      601 aacaaaacaa gatccagaag aagctaactt tctaacatcg ttgcatcttc atttaatgct
      661 taatgagcaa aatattgttt tagctactca tccaa
//
LOCUS       DANKHL010000380          677 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.398, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000380 DANKHL010000000
VERSION     DANKHL010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.398"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            50..677
                     /locus_tag="PW101_002939"
                     /pseudo
     CDS             50..677
                     /locus_tag="PW101_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
BASE COUNT          244 a          121 c          113 g          199 t
ORIGIN      
        1 aaaaaacgca tatagtagtc tcaaaactcc caagaaatga ggactacata tgcgttcatc
       61 aaaatatact gaacattata cagatacttt tataacattc aaggaaatta tgagaactgt
      121 ttctaacata taccacaatt gtgtaccaga taagattaaa aaccgaagaa atactgatca
      181 actgaagtaa cgcgatacag tgattattgc ttgtgttata tggggcataa ttaatggcta
      241 tactagccaa agagccacgt atagagcggt ttgttccgtc ttatttccta atggtgactt
      301 tcctagtagg agtcgattca ctcgcttaag ttcaaactta gcttacacta tcaagattat
      361 tcgatacttt ttcatcaaga agctcaccaa aggtgaacta gtcggaatta tagatagttt
      421 tcctagtcca ttatgtaaac cagttagaaa tagacaagca aaactattga atcaaatagc
      481 caaagttggc tataactcaa caaaaaaatc ttatttttat ggtcttaaaa ttcatatgat
      541 cgttactaag actggctttc cgattactta ctcaatcacg aatccaggtg tccatgacgt
      601 gaaagtgtta gagactttat cggaagaagc gaatcttccc aatatcctag gggacaaagg
      661 atatattagc cataaaa
//
LOCUS       DANKHL010000381          666 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.399, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000381 DANKHL010000000
VERSION     DANKHL010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.399"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            49..>666
                     /locus_tag="PW101_002940"
     CDS             49..>666
                     /locus_tag="PW101_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2515283.1"
                     /translation="MPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEE
                     NTLCSWKTDKSDAHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMD
                     LHNCLQLTFPELEQFFLNRLTPYALILIRLFPHPDFVLASTRTKIKNKLINETRKKIS
                     ANRAEQKADQIIHYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQK"
BASE COUNT          227 a          149 c          116 g          174 t
ORIGIN      
        1 gaacacactc aaagaggatt tcatgcatta ctcgaagaga tccagcactt gcctgaacgt
       61 ccattgattg tttttgaagc aacggggatt tattccagac cagtcgaaaa gttctttcaa
      121 gatcatcact tttcttactg tttgttaaac ccgttagaag cgaaaaaaca gatggaagaa
      181 aacaccttat gcagttggaa aacagataaa tcggatgccc atcggctcgc acagactcat
      241 cttaaaaatc aacgtcagcc aaaagaagta caaagcaatt tttacttaga gatgcgcgat
      301 ttagcccgtt tttatcaaga aattgagaag aaaatcactc gtctgcgcat ggatttacac
      361 aactgccttc agttgacttt tccagagctt gaacagttct ttttaaacag gctgacccct
      421 tacgcattga tattgatccg tctttttccc catccagatt ttgtattggc atctactcgg
      481 acaaaaataa aaaataaact gatcaacgaa acacggaaaa aaatctccgc gaatcgtgcc
      541 gagcaaaagg cagatcaaat catccactat gcacagtgtg cctatccagc tgttgaaaaa
      601 gacagtatcc actgtcaaaa aacgatctat tacgctgaac ttctccaaga tttactcgaa
      661 caaaaa
//
LOCUS       DANKHL010000382          633 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.402, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000382 DANKHL010000000
VERSION     DANKHL010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.402"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(122..499)
                     /locus_tag="PW101_002941"
     CDS             complement(122..499)
                     /locus_tag="PW101_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515284.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
BASE COUNT          192 a          110 c          111 g          220 t
ORIGIN      
        1 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
       61 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
      121 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
      181 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
      241 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
      301 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
      361 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
      421 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
      481 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt
      541 aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc
      601 gtttctgata aaacacgaac attaaaagaa tct
//
LOCUS       DANKHL010000383          601 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.403, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000383 DANKHL010000000
VERSION     DANKHL010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.403"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            178..576
                     /gene="spxA"
                     /locus_tag="PW101_002942"
     CDS             178..576
                     /gene="spxA"
                     /locus_tag="PW101_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL2515285.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          209 a           93 c          118 g          181 t
ORIGIN      
        1 agcgagaatg ctggttttac tgtatattaa ccttagataa ctttcatttt tttaaaaaaa
       61 ttatgaaaaa aaggtagcta tttaaaagtt gattatgtat aatagaaaca gatgggagat
      121 tattatgatc ttctgttaca aaaaagtgat tcttatcacg aaaaggagtg gtgcacgttg
      181 ttaacattat atacttcccc aagttgtact tcctgcagaa aggctcgtgc gtggttgcaa
      241 gaacatcaga ttccatttgt tgaacgtaat attttttcag aaccattgaa cagttctgaa
      301 ttaaaagcga ttttacaaat gacagaagac ggaacagaag aaatcatctc gacacgttcc
      361 aaagtattcc aaaaactgaa catggattta gatgatctgc cgttacaaga attattggaa
      421 cttgtgcaaa acaatcctgg attgcttcgc cgtccaatca tgattgatga taaacgtctg
      481 caagttggtt ttaatgaaga tgaaattcgt cggttcctac cgcgcgatgt acgacaatta
      541 gaattacgtc aagcacaatt gatggctgga ttatagaagg ataatacgaa acttggaaaa
      601 g
//
LOCUS       DANKHL010000384          593 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.404, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000384 DANKHL010000000
VERSION     DANKHL010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.404"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..>593)
                     /locus_tag="PW101_002943"
     CDS             complement(<1..>593)
                     /locus_tag="PW101_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515286.1"
                     /translation="VINLVNRKSTTKFANSFWFLENINETVEKNGVYKGDFHFQNAET
                     IEREHHYFDLLVNTVVELAKWAKSNETYSQVLKRIMSQETSFIYELSKSNLKYTYNED
                     ELEILDNPENSQWIKELKREDFIETLRILKSLFDLKLAKATIMGIERDSYYWEEIGLY
                     APQIEGEDKEKRIDNIINYINSLSEPLKCRGLDFIGT"
BASE COUNT          183 a          109 c           62 g          239 t
ORIGIN      
        1 aatgttccta taaaatcaag tcctctacat tttaaaggtt cacttaaact attaatatag
       61 ttaataatgt tgtctatcct tttctcttta tcttcacctt caatctgggg agcatatagt
      121 ccaatttcct cccaataata tgaatctctt tcaattccca tgatggtagc tttggctaat
      181 ttcaaatcaa ataacgattt tagaattctt agtgtctcaa taaagtcttc tctcttcaac
      241 tctttaatcc attgtgaatt ttctggatta tctaaaattt caagttcatc ttcattataa
      301 gtatatttca aatttgactt gcttaactca tagataaatg aagtttcttg actcataatt
      361 cttttaagta cttgactata agtttcgttt gactttgccc atttcgctaa ttctaccact
      421 gtattcacta ataaatcgaa ataatgatgt tctcgttcaa tagtctcagc attttgaaaa
      481 tgaaaatcac ctttataaac tccattcttt tccactgttt cattaatatt ttctaaaaac
      541 cagaatgagt ttgcaaattt agtcgtactt tttcgattaa ctaagttaat aac
//
LOCUS       DANKHL010000385          592 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.405, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000385 DANKHL010000000
VERSION     DANKHL010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.405"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(152..517)
                     /locus_tag="PW101_002944"
     CDS             complement(152..517)
                     /locus_tag="PW101_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515287.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          231 a           80 c          112 g          169 t
ORIGIN      
        1 tgctgcaatt attacacaac tgtttttagt gtttggagag aagaaaagaa gtaaaaaata
       61 gaaaaactag tgatagtttt ggtgatataa gaaaaacata tatgtataaa aagaagaaag
      121 tagcttctct ttgtacatat atggtttgtt tttatggatg ccgagctttt ttataagata
      181 aggctaccgc ccataacgac ccaaggattt gtggaactaa gtaaatacct gcaagaagca
      241 agatcagctg ggtcggtttt aaaggatgat agaagtaacg ccattcagta aaattggcgc
      301 aaaataaata ggaagtgaca gtagatataa tattggcaat gaataaagat ataatacgcc
      361 tgttcctttg aaaataatat ccagcaaaaa gagataaaaa tatcaatgtg atgaacccgc
      421 ttacggaatg ataacgaaaa tcaagcagta aggcgaaatg cacataagga acaaaagata
      481 aaagcaacgg aaggtttttc cagcaagttt ttatcatctt tcatcaccac ctaacaagtg
      541 ttattatttt gttagtttta ttgtaaattg aatcctgcaa taatcaatta ct
//
LOCUS       DANKHL010000386          590 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.406, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000386 DANKHL010000000
VERSION     DANKHL010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.406"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..>590)
                     /locus_tag="PW101_002945"
     CDS             complement(<1..>590)
                     /locus_tag="PW101_002945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515288.1"
                     /translation="QGKQDYQIIGYLEDDLNKIKINSDDVMYFFMTPLSDFPSSVIDN
                     VEGIQIYSNTLTKFGFIDDINESHSTRAQSLKEFLDPYIIVCTPKIKRNHEQARNYYQ
                     GDDLELIPKPKSEVFKNLIKENESLVPIPVFSINNSDSVIKDETSFENQLLNGKLLGK
                     IINFSKDNEDYPLNVFWENTDGSRVLFGEIIGQNNS"
BASE COUNT          186 a           90 c           82 g          232 t
ORIGIN      
        1 tgaattgttt tgtccgatta tttcaccaaa taaaactctt gatccatcag tgttctccca
       61 aaaaacattt aaagggtaat cttcattgtc ttttgagaaa tttataattt ttccgagtag
      121 tttgccgttt agtaattggt tttcaaaact tgtctcgtct ttgattacag aatcgctgtt
      181 atttatagaa aagaccggta taggtactaa tgattcattc tcttttatta aatttttaaa
      241 aacttcagac tttggtttgg ggattaattc taaatcatca ccttgatagt aatttcttgc
      301 ttgttcatga tttcttttaa tttttggtgt acaaacgata atataaggat ctaaaaattc
      361 tttaagtgat tgggcacgag tactatgaga ttcgtttata tcatcaataa acccaaattt
      421 agtaagcgta ttactatata tttgtatacc ctctacatta tcaatcacac tactggggaa
      481 atctgaaaga ggagtcataa aaaagtacat tacatcatcc gagtttattt taattttatt
      541 taaatcatcc tccaaataac caataatttg gtaatcttgt ttaccttgtt
//
LOCUS       DANKHL010000387          575 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.408, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000387 DANKHL010000000
VERSION     DANKHL010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.408"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            312..>575
                     /locus_tag="PW101_002946"
     CDS             312..>575
                     /locus_tag="PW101_002946"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDL2515289.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLS"
BASE COUNT          194 a          106 c           82 g          193 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctccccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataaccaa accattcaga
      541 atctcatgaa tggtttggtt atcaaattcc tttcc
//
LOCUS       DANKHL010000388          575 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.409, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000388 DANKHL010000000
VERSION     DANKHL010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.409"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
BASE COUNT          186 a           81 c           81 g          227 t
ORIGIN      
        1 tttttcttga tgtgatatcc ttgtcttttt tgtaatgttt ttaacttatt gcttttgcaa
       61 aatttattta cctgttttct ggatcaacga tagtgtacat tatgctatcc ttgtttggat
      121 tttaggacgt ttaaagaggc acttttttac ataacttaat ccatagacct tttaagcatg
      181 ctgtgagata ataataaaga agagtttaaa gcgcacccca aaccacttcc ccataagtgt
      241 attacgcttt aaactctttt atatttgaat tcactaagaa gcataccata tttttgaaaa
      301 aaagtgagaa aaaaggctta taattaggat ttagagtaat taattagatg tttattttat
      361 aggactgata ctatagatta caaatagtat cgtatttcgc aatcttaaaa ttatcttctt
      421 actgtgaata agataataaa atttgtattt gtccttttta ggtaacttat tttaatctat
      481 atacctttct ggatcaacga aagtatactt tatatagtca taacgccgat ggtaactatg
      541 cactgtaacg ataatgctgc taaaatatct tttta
//
LOCUS       DANKHL010000389          569 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.411, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000389 DANKHL010000000
VERSION     DANKHL010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.411"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..>569
                     /locus_tag="PW101_002947"
     rRNA            <1..>569
                     /locus_tag="PW101_002947"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          139 a          131 c          173 g          126 t
ORIGIN      
        1 gagagtggaa ttccatgtgt agcggtgaaa tgcgtagata tatggaggaa caccagtggc
       61 gaaggcggct ctctggtctg taactgacgc tgaggctcga aagcgtgggg agcaaacagg
      121 attagatacc ctggtagtcc acgccgtaaa cgatgagtgc taagtgttgg agggtttccg
      181 cccttcagtg ctgcagctaa cgcattaagc actccgcctg gggagtacga ccgcaaggtt
      241 gaaactcaaa ggaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgaa
      301 gcaacgcgaa gaaccttacc aggtcttgac atcctttgac cactctagag atagagcttc
      361 cccttcgggg gcaaagtgac aggtggtgca tggttgtcgt cagctcgtgt cgtgagatgt
      421 tgggttaagt cccgcaacga gcgcaaccct tattgttagt tgccatcatt cagttgggca
      481 ctctagcaag actgccggtg acaaaccgga ggaaggtggg gatgacgtca aatcatcatg
      541 ccccttatga cctgggctac acacgtgct
//
LOCUS       DANKHL010000390          539 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.413, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000390 DANKHL010000000
VERSION     DANKHL010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.413"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
BASE COUNT          183 a          104 c           65 g          187 t
ORIGIN      
        1 aaataaacat gtgccatcta tctgttggca catgttttag gatttatggt actttttatt
       61 cggcaatcca ttttattttt ctacttcttc ttaatacctc taacactttt gatttataaa
      121 ctaatccaat gcatctactt gcgcttatta agctatttca gctagatagt accaccccat
      181 gcgtcaatgc ttgattttcc ttttgtataa cattgtataa gcaataaagg acaaataagt
      241 ggtgattaca ccaaaccatt gaataagaaa aattctgata cgtaaataaa catgaacttg
      301 aaaaaattaa tgaatcggtt agctactacg tagttgtctt ttttctcata cagttctgtt
      361 aacttacact tctcttcgct agataataaa aaatcctcca actgtattgt ccaatataat
      421 cccaggcatc taaactagca ttatagaaga gacacacata gaatctaaaa taactggttt
      481 taaatgtttc ttacaatcaa aaaaagctgt taaatgcact ccacaccttc atactctat
//
LOCUS       DANKHL010000391          521 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.415, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000391 DANKHL010000000
VERSION     DANKHL010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.415"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
BASE COUNT          177 a           98 c           59 g          187 t
ORIGIN      
        1 tatataacac agttattttt aaaaataaga atgctaaatc actctacaat aacggcgaaa
       61 gcacaaaaaa taaaaaaatt attacgttat taaaaaaaca gatgaaagat caggttttat
      121 tagatctcat tctttcatca cttgaaggga tttaatgaac tactttttcc tctttgtcgt
      181 ttttatatat tcgtattcct tcaaacagta ggatagcctc tttcaatgct tgatatttga
      241 actgtccgtt acttaaaaat ttagatgtgc cgccctcttc ctagaacata ttctttttta
      301 ctttttcatt aattgacaga ccaatcgaac gaaactgtaa atttcgatag gataatgatt
      361 ggtcaaactc attcattttc aagacatctc gattcatatt caagatagga atctttttat
      421 caacatcact ttattcgtat tttcctctac cataaaagct tcacgctcca caaaattgtt
      481 ttatacacta ctattcagcc cattcaacga ccaatgaatt t
//
LOCUS       DANKHL010000392          515 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.416, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000392 DANKHL010000000
VERSION     DANKHL010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.416"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(34..>515)
                     /locus_tag="PW101_002948"
     CDS             complement(34..>515)
                     /locus_tag="PW101_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2515290.1"
                     /translation="SKDDKIVMGKRIREVRKNREETLEKFGKNFTIYTGKNVISRWEK
                     GINIPDIEKLMNIAYLGKVTVPYLMYGEDYKNILPKDERVSDFKKINSFSMGLRMRKI
                     RKDYYLEREEFGKLFSPSISKWSIDRYENGRDIPNTNRIIQYAYIGNLSLEFLIYGI"
BASE COUNT          158 a          100 c           57 g          200 t
ORIGIN      
        1 cgataactta ataagtatcg acgtaaacta tttttatatc ccgtaaatca aaaattctaa
       61 agaaagattt cctatataag catattggat aattcgatta gtattgggta tatctctacc
      121 attctcatac ctatctatgg accattttga aattgaagga gaaaaaagct ttccaaactc
      181 ctccctttct agataataat ctttcctaat tttcctcatc cttaatccca tactaaaaga
      241 atttatcttt ttaaaatcgc taactctttc atcttttgga agtatattct tataatcttc
      301 tccgtacata aggtaaggaa cagtgacctt accaagatag gcaatattca ttagtttttc
      361 aatatctgga atattaatac ctttttccca tctacttata acatttttgc ctgtatagat
      421 ggtaaagttt ttaccaaatt tttcaagcgt ctcttcccgg ttctttcgga cttctcgtat
      481 tctttttccc ataacaattt tatcgtcttt tgata
//
LOCUS       DANKHL010000393          514 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.417, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000393 DANKHL010000000
VERSION     DANKHL010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.417"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(34..234)
                     /locus_tag="PW101_002949"
     CDS             complement(34..234)
                     /locus_tag="PW101_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2515291.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          170 a           79 c           92 g          173 t
ORIGIN      
        1 aaataacccc tgtatccttt gcggtacagg gggttatttt gtataagtag acattgtagt
       61 ttctaaactg ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag
      121 taaaatagat gcatgcgtat gtcttagtcc atcaataaac agtcgaagag aaaataggga
      181 aaaattgatt actaaagaaa aacttactag gtcgacggtg tagttgattt tcatacttca
      241 atagattcga attaaacgat ttcgattggc ttataccagt cctatccaac gaaagatata
      301 tcgttcttaa ctctagcggt ataacttgaa gctttacgta aagccaatgg gaatgaactg
      361 gagatgactt tttattattt ttagtaatag ctgatgggtt aatacttaca actttccttt
      421 tcaaagtacc atataaaaaa acggtggggt tgtttagcga aataaaggat ctaagccaat
      481 agcttagatc ctttattcta tgctttatat tatt
//
LOCUS       DANKHL010000394          509 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.418, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000394 DANKHL010000000
VERSION     DANKHL010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.418"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..132)
                     /locus_tag="PW101_002950"
     CDS             complement(<1..132)
                     /locus_tag="PW101_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL2515292.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DANKHL010000395          488 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.420, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000395 DANKHL010000000
VERSION     DANKHL010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.420"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            55..249
                     /locus_tag="PW101_002951"
     CDS             55..249
                     /locus_tag="PW101_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515293.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          206 a           62 c           84 g          136 t
ORIGIN      
        1 tcacttacat atatttgaat attaactaaa aacagcaaga aagaagagga tcaaatgtat
       61 tatgtagaag taaaaacaaa aggcgtcaaa aacaaacaat atgtgaaagg tatcagtaac
      121 gaatatcctt tacttggttc atggaaagaa gctgctcctt tttccaaacc atgtgccata
      181 aaaataaaga acgagctaga aaaagaactg acttgtggta aagctgttgt agatatcatc
      241 gaaaagtaaa tccaatgtat agaaaggaag atcacacatg aaaagcgacg tacaaattgc
      301 taagattata gaattagaag aagaagacgc agtgttgaca attgaaaaaa aataaatgtt
      361 tgtataccat atgattattt caatatgtat cctggaattg gagatctcgt agagctatat
      421 caagatgaat caaatatact tgtcattcat catgaaatat agagacttat aaaattgttt
      481 atttttat
//
LOCUS       DANKHL010000396          455 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.423, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000396 DANKHL010000000
VERSION     DANKHL010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.423"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..>455
                     /locus_tag="PW101_002952"
     CDS             <1..>455
                     /locus_tag="PW101_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2515294.1"
                     /translation="ETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQL
                     TKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKKNWRFLLKN
                     RASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV"
BASE COUNT          150 a           95 c           90 g          120 t
ORIGIN      
        1 gaaacaggaa acttaattgc tgtcttgcct acgcgtaaat tacctcgatt aacaagctat
       61 ttcttaggct gcaccaatcc agaagaagta gaattcttag tcacagacat gaacgccgct
      121 tacttccagc ttaccaaacg tgttctgcca aatgcgaaag tagtgattga tcggtttcat
      181 attgtcaaac acatgaatca ggcactcaat gagttgcgta tccgtgaaat gaatgagctt
      241 cgtaaagcag gacagaaaag tcaggcagaa aaactgaaaa agaactggcg ctttctgcta
      301 aaaaatcgtg caagcatcaa ccattatgag tacaaaacat ggaaaagttt ccgagcacca
      361 aaatacccat ttctcactga agcaatgatg attgatcgat tgcttgagtt ttctgcgcct
      421 ctaaaggagg cgtatccctt ttttcatgaa ttagt
//
LOCUS       DANKHL010000397          446 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.425, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000397 DANKHL010000000
VERSION     DANKHL010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.425"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
BASE COUNT          150 a           76 c           70 g          150 t
ORIGIN      
        1 aaataagctg aaattcacaa aaatttgaaa agcaatttcc gtgaatttct tcttatttct
       61 tggagttaaa cgcttctgtt ccagcctctt tttatctgtc tttcactaat gatcagttgt
      121 agataagcaa gttgcttttt cttacatcat accgcccatc aagacaaacg tttttagctt
      181 gtacgtactg gacaaaaatc ccattatatc aagaaaaaac aacgttgata atacttaaaa
      241 ttattaggct agaacggact agtaagagtt ttttgcccct tttttgcccc tcaaatatcg
      301 ctttacagcc agaacataag ttcgtataat cgttttgagg tgatttttat gatggaagac
      361 tttattagaa aaaatatcag tgatgaatat gcagactttt atgagcaaag cagcaaaaaa
      421 gataaattcc agatggatgt ttcaat
//
LOCUS       DANKHL010000398          441 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.427, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000398 DANKHL010000000
VERSION     DANKHL010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.427"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
BASE COUNT          170 a           62 c           92 g          117 t
ORIGIN      
        1 cttgttgaat tctagtaatg aagcgaccct aaaacttaga ccaaaaaatc taacttttgg
       61 gtggagttgt caagaaaaac cgggcactta gttaaaaaat aagttggacg ggttatacta
      121 aaattgacac caaattaaga gaaagcgaag atctggatta tgccaagacg ttcatttgat
      181 gaaatattta aaattaccgc tgtaaaactg gtaacggaag aagggttttc tgttaaagaa
      241 gttagtctat agctagaagc tcgctctaat agcgtgtatt gatgagttca agaagttgaa
      301 aaatatggat aacgtacttt tctcggtaga ggaagtcatg aaatccattc gcagaaatga
      361 aattacacgc ttagaaaaag agaataaacg gttaaaagaa taggtagaac tcctaaaaat
      421 taccgaagtc ttcctgaaag a
//
LOCUS       DANKHL010000399          414 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.428, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000399 DANKHL010000000
VERSION     DANKHL010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.428"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
BASE COUNT          150 a           62 c           50 g          152 t
ORIGIN      
        1 gtaattccta attttgctcc acttttagat aatttttagg tctatttaac tcatcagaca
       61 ttctatgctc atttttctcc aaatttgttt caactctttc gtacttaatt ttgcgtattt
      121 gaaaattctt cttttagagt acttttatta cgatactagt atgatatata tctatagcaa
      181 acagttaaaa tcttcctaaa ataagcgaat agcattccta aaacctataa aatttttaga
      241 tgaagcgaca agtggacaat taataaacta cttgaacatg aaattatgga tcacctttca
      301 aatttagaaa atggaattct tatacttatt gcgcaacatt tattgattgc aaaattatgt
      361 gacaaaaaaa actagtagaa gaaagaataa tggcttgata ataaagtact acct
//
LOCUS       DANKHL010000400          408 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.429, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000400 DANKHL010000000
VERSION     DANKHL010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.429"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..>408
                     /locus_tag="PW101_002953"
     CDS             <1..>408
                     /locus_tag="PW101_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HDL2515295.1"
                     /translation="QDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEY
                     GKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQA
                     AYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL"
BASE COUNT          138 a           67 c           83 g          120 t
ORIGIN      
        1 gcaggatgtg attattgtag ccgtgggcta ctatcttcgt tataacctta gctatcgtga
       61 agttcaagaa atcttatatg atcgtggcat taacgtttct catacgacga tttatcgttg
      121 ggtgcaagaa tatggcaaac tactctatca aatttggaaa aagaaaaata aaaaatcctt
      181 ttattcatgg aaaatggatg aaacgtacat caaaattaaa ggaaaatggc attatttgta
      241 tcgagccatc gatgcagatg gtttaacctt ggatatttgg ttacgtaaaa aacgggacac
      301 acaagcagcc tatgcttttc ttaagcggtt agtgaagcag tttgatgaac cgaaggttgt
      361 agtcacagat aaagccccct ctattacaag tgcctttaag aaactaaa
//
LOCUS       DANKHL010000401          397 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.430, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000401 DANKHL010000000
VERSION     DANKHL010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.430"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
BASE COUNT          167 a           52 c           69 g          109 t
ORIGIN      
        1 ttaaggtgtc actaaaaatt ataaaaaata gtgttgacgc acttttgatg tcatgttata
       61 ataacgaagt cgtttggatg agatagctaa taaagcttga ttttatcgaa aaaaacttcg
      121 aaaaagttat tgacaaataa caataaagat ggtatgataa tcaagtcgct gctaatgagt
      181 ggcaaagacg tttagaaaaa ataaacaaag aagttgttga cattctcttc tgaatctgat
      241 aagattaaag agttgttaaa acaaaaagat tagacctttg aaaactgaac aaagtaagac
      301 aaaccaaatg tgtagggcgt cttgattcaa ttcaagacaa caaacatttt taacaagcaa
      361 gcaatatgct agcaaacaaa ttgagcttaa caatcgc
//
LOCUS       DANKHL010000402          390 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.431, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000402 DANKHL010000000
VERSION     DANKHL010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.431"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(<1..244)
                     /locus_tag="PW101_002954"
     CDS             complement(<1..244)
                     /locus_tag="PW101_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2515296.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAF"
BASE COUNT          151 a           75 c           65 g           99 t
ORIGIN      
        1 gaaatgcttg atccgaagaa aataaaaatt tgaaattttc ccaacccacc caaggacttt
       61 ccctcaagct agccaagaac ccatctgctg aaatatgaaa atttttgaat gctacgacat
      121 tcgccattac aggtatgtaa aagaaaaaaa tcatccaaag catacccggt acagccatca
      181 ggacaagtgc tttataacgc catagattca taaaaaaatg attgaacccc tttcgttttt
      241 tcatctaaaa acccctttcg attcattcgc aacgtaatta tagcgctttc aataatgaga
      301 ataaataaac aaagtatagg gaaaatgaaa caaagtatag gagaggttgt gaaatcagcg
      361 ttttggcctg agtattagag aaaaaaacgg
//
LOCUS       DANKHL010000403          343 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.433, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000403 DANKHL010000000
VERSION     DANKHL010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.433"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
BASE COUNT          112 a           65 c           56 g          110 t
ORIGIN      
        1 ctgttctaac attctcaact ttgtcagttt accataggaa aatgggaaaa tctacgaaag
       61 ctctcaacag aatcgtccta cttaaatacg cttttcttta ataataaata agttggttgt
      121 ttttgttatt tgcgctttca cttttcggaa taaataatgt gtatttatat ggaataaata
      181 aaaaaagagg tcgtgactaa aaacaagtca caacctctgg taaacggtgt tctcctgaca
      241 actcctcggt cacaagacac aatattcaac aaacatgaga agctattttt tgaaattgct
      301 ctttgtgact cagagtgaaa cggcaggctc acgacctctt tct
//
LOCUS       DANKHL010000404          342 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.denovo.434, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000404 DANKHL010000000
VERSION     DANKHL010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.denovo.434"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            70..>342
                     /locus_tag="PW101_002955"
     CDS             70..>342
                     /locus_tag="PW101_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515297.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT          152 a           52 c           51 g           87 t
ORIGIN      
        1 ccttcttgtt ttcatataac caaaggcgct atgatttcca tatgaaaaaa ataaagtcag
       61 gtgaaaaaaa tgaaacttga ggataaaaaa gtcattttga agaaaatcaa acgactacca
      121 gaccaaatag atggctatac gatctgttct ttcaaaaaag acaggagtat aaaagcttat
      181 acgcaaaaca cagaaaaagg aataatctat ttcctacatg aagaaggtta cgatacccaa
      241 acctttatgt ttactgaaaa aaaagaattt cataaacaga tgaaaaaact gattgaaaaa
      301 gaatttccaa gaagtcataa actctatgtg aatcagcaat tt
//
LOCUS       DANKHL010000405        29807 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.guided.005, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000405 DANKHL010000000
VERSION     DANKHL010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.guided.005"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(39..1439)
                     /locus_tag="PW101_002956"
     CDS             complement(39..1439)
                     /locus_tag="PW101_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HDL2515298.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            1657..3318
                     /locus_tag="PW101_002957"
     CDS             1657..3318
                     /locus_tag="PW101_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDL2515299.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            3275..3973
                     /locus_tag="PW101_002958"
     CDS             3275..3973
                     /locus_tag="PW101_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL2515300.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            3989..4510
                     /locus_tag="PW101_002959"
                     /pseudo
     CDS             3989..4510
                     /locus_tag="PW101_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            4767..4955
                     /locus_tag="PW101_002960"
                     /pseudo
     CDS             4767..4955
                     /locus_tag="PW101_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            5001..5966
                     /locus_tag="PW101_002961"
     CDS             5001..5966
                     /locus_tag="PW101_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2515301.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            5988..6200
                     /locus_tag="PW101_002962"
     CDS             5988..6200
                     /locus_tag="PW101_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDL2515302.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            6275..6751
                     /locus_tag="PW101_002963"
     CDS             6275..6751
                     /locus_tag="PW101_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HDL2515303.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            6748..8556
                     /locus_tag="PW101_002964"
     CDS             6748..8556
                     /locus_tag="PW101_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDL2515304.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            8633..8848
                     /locus_tag="PW101_002965"
     CDS             8633..8848
                     /locus_tag="PW101_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HDL2515305.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            8940..9803
                     /locus_tag="PW101_002966"
     CDS             8940..9803
                     /locus_tag="PW101_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515306.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            9840..10376
                     /locus_tag="PW101_002967"
     CDS             9840..10376
                     /locus_tag="PW101_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HDL2515307.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            10390..10812
                     /locus_tag="PW101_002968"
     CDS             10390..10812
                     /locus_tag="PW101_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HDL2515308.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            10742..13141
                     /locus_tag="PW101_002969"
     CDS             10742..13141
                     /locus_tag="PW101_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HDL2515309.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            13173..15164
                     /locus_tag="PW101_002970"
     CDS             13173..15164
                     /locus_tag="PW101_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2515310.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            15187..15438
                     /locus_tag="PW101_002971"
     CDS             15187..15438
                     /locus_tag="PW101_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HDL2515311.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            15428..16657
                     /locus_tag="PW101_002972"
     CDS             15428..16657
                     /locus_tag="PW101_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HDL2515312.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            16654..18735
                     /locus_tag="PW101_002973"
     CDS             16654..18735
                     /locus_tag="PW101_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HDL2515313.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            18884..22804
                     /locus_tag="PW101_002974"
     CDS             18884..22804
                     /locus_tag="PW101_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HDL2515314.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            22805..23749
                     /locus_tag="PW101_002975"
     CDS             22805..23749
                     /locus_tag="PW101_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515315.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            23753..23956
                     /locus_tag="PW101_002976"
     CDS             23753..23956
                     /locus_tag="PW101_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HDL2515316.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMLVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(24256..24492)
                     /locus_tag="PW101_002977"
     CDS             complement(24256..24492)
                     /locus_tag="PW101_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2515317.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(24675..25061)
                     /locus_tag="PW101_002978"
     CDS             complement(24675..25061)
                     /locus_tag="PW101_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HDL2515318.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(25043..26371)
                     /locus_tag="PW101_002979"
     CDS             complement(25043..26371)
                     /locus_tag="PW101_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL2515319.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(26332..26661)
                     /gene="mobC"
                     /locus_tag="PW101_002980"
     CDS             complement(26332..26661)
                     /gene="mobC"
                     /locus_tag="PW101_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HDL2515320.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(26919..27290)
                     /locus_tag="PW101_002981"
     CDS             complement(26919..27290)
                     /locus_tag="PW101_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2515321.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            27435..27620
                     /locus_tag="PW101_002982"
     CDS             27435..27620
                     /locus_tag="PW101_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL2515322.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            27778..27945
                     /locus_tag="PW101_002983"
     CDS             27778..27945
                     /locus_tag="PW101_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515323.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            28132..28350
                     /locus_tag="PW101_002984"
     CDS             28132..28350
                     /locus_tag="PW101_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515324.1"
                     /translation="MRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            28563..29222
                     /gene="vanR"
                     /locus_tag="PW101_002985"
     CDS             28563..29222
                     /gene="vanR"
                     /locus_tag="PW101_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HDL2515325.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALTDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            29225..>29807
                     /locus_tag="PW101_002986"
     CDS             29225..>29807
                     /locus_tag="PW101_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDL2515326.1"
                     /translation="MERKGIFIKVFSYTIIALLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAH"
BASE COUNT         7708 a         7632 c         7937 g         6530 t
ORIGIN      
        1 cttatttctt gaagctgacc acttctgtca caacctcttt aagaatgatg gatagaaata
       61 cgtttaggat aagttttttt agatgcactt tgttcgattt cctctaaaga tttcccacga
      121 gtttctggta cgatctcttg aataaataaa acccctaaaa agcagatgat cccaaaaatg
      181 ccgaaaacga tttcttgaga catagaagca gtcatcacag gaaaaagcaa gccaactagg
      241 aaagaaccaa tccaattaaa agaagaggct aggccagatg ctcgcccgcg gacagctagt
      301 gggaatattt cgcctatgat tacccatgtc agtggagccc atgtgcaaga ataaaatgcg
      361 acataaatac ataaaaacaa gagaatcaac aacgaatttg tatgagcatc cagtacagtt
      421 cctaaaacag ctggtaggat gaatgaaagt cccatcacgc taccgcctat tttcaataaa
      481 gttcgccgat tgaacttgtc tgcaataacc aaaaagagca aagaaccagc aactaaaatg
      541 attccttgga tgatcggcca catcaatgca tcgctcgctg catgcccagt cgctttttct
      601 acgatcagcg gaatatagta aaaaatagca ttagctcctt gaaattgctg gaatactgct
      661 acaccaatac cggcaatgac taaggaacga tatttttcta aaaacaaagt atgccaagaa
      721 gttccggttg tcttttcttg tttgacattc agctggattt gttcgaattc ctgttcagct
      781 tcttttggtg tccgaatata gttcaatact ctttttgcct cttccaagcg gccagattgc
      841 ataagaaaac gcggtgactc tggtagagct aaaacaccga aaaataaaat cagcgcgggg
      901 acagcagcga gccctaacat cacgcgccaa gccatcgttt caggcaacac ttttagcaaa
      961 taatctgcaa cataggaaag gagcatgcca gaagcaatca tcacttgatt gatccctgac
     1021 aatcttcccc ttagtctagc aggagccatt tcagacatat aagcaggaac tagggcagat
     1081 gcagcgccaa cagctaaacc tagaagaatc cttgctgcaa tcagaaaata agaaccattg
     1141 tgaggggcag atgcagaaag gacagaacca gccataaaaa taagtgcaga aagaagaatc
     1201 atttttcgtc gtccaagctt atcagaaaga agaccagata aagagccgcc aaggatcgca
     1261 cctaacatga ccgaagacgt gatccatcct atcaaagaag ctttacctgc caaaccccaa
     1321 tcatgctgta aaaaaggtaa ggcgccggtc ataacaccaa tatcatatcc aaatagaatg
     1381 ccgccaaatg cgccaaaaaa ataaataaac gatgaggaaa cttttttttc ttttttcata
     1441 agtattctct ccttctagaa tttcgagaag gcggttgatc agattttgta agcaatttca
     1501 ttttataact tatgtacgta caaaacaagt gttttttatt gtattcttgt aaataaccgc
     1561 ttgacatgta cgtacaaaag tatacaataa tcattgtaag cgctactaaa aagacggttt
     1621 gatcaggttt gacgagaagg agggagttaa atcattatga aggaagtaaa tgcgcaaagt
     1681 atgttagatg tcatagatgg tcgtacatct ttagggattg agtttggatc aactaggatc
     1741 aaagctgtat tgattgattc atcttttact cctattgccg ctggtagtta tgagtgggaa
     1801 aacaaactag aaaatggtat ctggacctat tcattagaag atatttggaa aggactgcgc
     1861 gtcagttacc gaaaaatggc agcagaagta ttcgaaatct atggcgagaa tcttacgaca
     1921 atcgggtcga ttgggttcag tgcaatgatg cacggttatc tagcatttga tagcaaggaa
     1981 cagttactag taccatttcg tacttggcga aatacaatca ctgaagaagc agaacgaata
     2041 ttaacggaaa aatttcaata caatattccg cagagatgga gtatcgctca tttgtatcaa
     2101 tctattttga ataaagaaga acatgtgaaa gaaattaatt ttttgacaac gcttgcaggt
     2161 tatgtccatt ggcagcttac tggtgagaaa gtactaggcg taggagatgc atcaggcatg
     2221 tttcctatcg atccttcaac gaaaacctac catcaagaaa tgaaaaatat ttttggtcag
     2281 ttaatcgaaa aagcgcacgt agcgattgac ttggatcaca ttttgcctaa agttctagta
     2341 gcaggagaag aagctggcaa gttaagcgaa tcaggggctt atctgatcga ccctacggga
     2401 aatttgagac caggaatccc ggtttgtccg ccagaagggg atgcaggaac cggtatggtc
     2461 gctacgaaca gtgtagccaa acggacaggg aacatttcgg ctggtacttc cgccttcgct
     2521 atgatcgtat tagaaaaaga actagaaaat gtccatccgg aaatcgacat ggtcactaca
     2581 ccttcaggag aactagtagg catggttcat acgaataact gctcttcgga tatcaatgct
     2641 tgggtcagta tttttgaaga atttaccaat tctttgggta tggagataag cagagataaa
     2701 ttattcacgg tattattcaa caaagcgcta gaaggcgata cggattgcgg gggattactt
     2761 tcttatggtt atttttccgg tgaaaacatt acaggagtaa atgaaggaag gccattattt
     2821 gtacgtacac caggtagccg ttttgattta gcgaatttca tgcggatcca tttagccagt
     2881 gcttttggtg caatgagaat cggaatggat atattgaaat ctgagaatgt ccaaattgat
     2941 cgtttagtgg ggcatggggg gatttttaaa acaccagaag ttggacagcg gattctagca
     3001 tctgctatgg aggcaccagt tacagtgatg gacacagccg gagaaggtgg agcgtgggga
     3061 atcgcgttat tagcagccta tatgatggat cgacatggga agtctttaga aaacttcttg
     3121 gcagaagacg tgtttggaca agaccaagga atcaccatca caccttcagc agaagaaata
     3181 gcaggctatc agatatttat gagacgttac cgtgagggga tcgacattga aaaaacagcg
     3241 attcatcaat tgcaagaaaa aggagtgata gaaaatgctg gaacaactga aagaagaagt
     3301 ataccaagca aatcttgact tgcctaaaca cggactcgtc aaatatacat ggggaaatgt
     3361 gagcgctttt gatcctgaaa cacgatattt cgttatcaaa cctagcggag tcagttatga
     3421 agagatgaca gcagacgaca tggtcgttgt ggacttggac aatcatatca ttgaaggtaa
     3481 actcaatccc tcttcggata cgcctactca tgctgtttta tatcgttcat ttcctcaaat
     3541 cggaggaatc gtccatactc actcgacatg ggcgacgatc tgggcacaag caggattgga
     3601 tgttcctgcg atggggacga cgcatgcaga tactttttat ggatcggtac cttgtgctcg
     3661 atttttgaca caacaggaaa tcgacagcgg gtacgaatat gaaacaggaa aagttatcat
     3721 agaaacattc aaagaacgca aaattgatcc cttagcaatt ccaggggtac ttttacacgg
     3781 acacggtcca tttacttggg ggaaagatgc gcaaagtgcg gtaatgaacg cagtcgtact
     3841 agatgaagta tgcaagatga atttatttac cagacagctc aattcatttt cagaagagct
     3901 gcctcaacgg attttagata aacattattt acgaaaacac ggaaaaaatg cgtattacgg
     3961 acaaaacaaa taaaaggagt ggctaattat gttgaatatt ggagaaaaag aattttggtt
     4021 tgttgtagga tcacagcatt tatatgggga agaagcactg cgagaagtaa agaaacatgc
     4081 acaagaaatg gtcgatgaac tgaatgaaaa tggccaattg ccttatccaa tccgccttca
     4141 agaattagca gttactgctg atacgatcac aaaaatcatg aaagaggtca attatcgaga
     4201 agaagtagca ggtgtgatca cttggatgca tactttctca ccagcaaaaa tgtggatccg
     4261 cgggacaaag cttctacaaa aaccattgct tcatttagct acgcaatata acgaaagcat
     4321 tccttggaaa acgatcgata tggatttcat gaatttaaat caatccgctc atggtgaccg
     4381 cgaatatggg ttcatcaatg ctcgattaaa taaacaaaat aaaatcgtcg ttggctactg
     4441 gaaacgacct gagatccaaa aagagattgc tgattggatg gatgttgcag tagcctataa
     4501 tgaaagtttt attccaaaaa tttaggttat cgctgaaagc cccggaaata cgggcatttc
     4561 gcaggatatg agaactcaat ttactaccaa tttactactt tcggattgag ccgcatatat
     4621 gctgaaccat actgctaata cccgccaccc caaatacggc acatcataaa ttgaatacga
     4681 caccgtaaac gcggcgttcc tctttcccgt catgggagaa caggacgccg cttttttatg
     4741 cccaaataga gaaaggaggc gacgccatgc tatttacttc cggccctgac cgggcgtttg
     4801 aacggctgat gcaggaaaag cccggtttcg atcactacga cggcggcggg gcggacgcgc
     4861 cggaggattg cggcacctgc cgattttacc gcccggattg gaaatatcaa ttctgccagt
     4921 ttgcagagtg tccctatcag ccgggcaagc tgaccgacta tgcagcggtc aaattcacag
     4981 tgaaaggagc gaacaacgct atggcagttt tccgtgtgga aaagaacaaa ggctatacgg
     5041 tgatgtcaaa tcaccacctg cggaataaga acctgacctt aaaggccaag ggcctgctgt
     5101 cgcagatgtt gtccttgccg gaggattggg actataccct aaaaggactg tcctatatca
     5161 accgggaaag catcgacgcg atcaggacag cggtatggga gcttgaaaaa gctggatata
     5221 tccggcgtga gcagggccgt gacgcaaaag gcaaaatggc cgatatggtc tataccatct
     5281 acgaacagcc ggtattggaa aacccggtat tggaaaatcc aacatcggat aacccggtgt
     5341 tggaaaatcc gacatcggat aatccggcgt cggaaaatcc aacgcaatta aataaagaag
     5401 tacaaagaac tgacttatca aaaaaagaaa aaataattac agatggacaa agtacccatt
     5461 ccattcctat cccatccccg acccctgccc ctatggaggg cgaggcagcg gctacgccgc
     5521 cggaacggaa aaggacagga aaggatgagg ctgtacggat ttatcgggaa atcattttgg
     5581 agaacatcga gtatgcctat ctcattcagg acaggagcat tgaccgtgaa cagcttgacg
     5641 aaatcgtaga cctgatgctg gaaaccgtct gcaccgcccg aaagacaatc cgtgtcgccg
     5701 gggacgacta ccctgccgag ctggtgaaat ccaagtacat gaagctgacc ggcgaacata
     5761 tccgctttgt ccttgactgt ctgcgggaga acacaacgaa agtccgcaat atcaaacaat
     5821 acctgcgggc tatgctgttc aacgccccca gcaccatttc caactactac acggcccttg
     5881 tcgcgcacga catggcacag cccgattggg gaaagcctaa atccggcctg ccggactatt
     5941 cctgcggcga gggcgaaagc ctgtaacgag aaaaggagga cgaccctatg aagcagggcg
     6001 cattgatttt tgacgagcga acagaccgtt acgacatccg ctttgatctg gccgactatt
     6061 acggcggcct gcattgcggg gagtgtttcg acgtgatgat cggcggcaaa tggaggccca
     6121 cccgcattga aatggccgaa aactggtatc ttgtcggcat ccgggccgac gacctttccg
     6181 gcctgcgggt gcggatttga cctatatcta cctgcggcgt ctggtggttc cgccttagac
     6241 gccgcttttg actttctgac aaggagggat gcgattgcag gaagaagtta ctcaaaagac
     6301 cattgccctg tccatgaaaa cgggcaagct cacggctcaa gtgctgcaag cggcgctgaa
     6361 aaagtacctg caacaccggg ccaagggcaa aaacacgctg caccacggcc agcagagcct
     6421 aaagcagctt aaaaagcatg gggctgatct ctccaacatt gagatcaccg aggccaacat
     6481 tggagcgttc aagccatgcg ccaagaaata cggcgtggat tttaccttac gaaaagataa
     6541 gaccacccag ccgccccact acgttgtgat tttcaaggcc aaggatgcag ataaccttga
     6601 tctggcgttt cgggagttta ccgccaagac gctttccaag gagcagcggc cctctatccg
     6661 caaggttttg tccgctgtga agcaaaaggc ggcggcccag accaagcagc gggccaagga
     6721 gaaaatcaag gaaagggggc tggaactgtg aagcctgacg taaagaaatt gctgatcctg
     6781 aacctcccgt atctgatttt tgtgtacctg tttgcgaaat gcggggaggc gtaccggctg
     6841 gccgctggtg ctgacctgtc ggggaagctc ctgcattttg cggatggttt tgcggcggct
     6901 tttgccaacc cgctgccaag cctgcacctg tttgacctgt gtatcggtat cgccggtgcc
     6961 gtgatcgtgc ggctggtggt gtactgcaag agcaagaacg tgaagaaata ccgcaaaggc
     7021 atggagtatg gcagcgcccg ctgggggacg gccaaagaca tagcgcctta catcgacccc
     7081 aagtttgaaa ataatatcct gctcacccaa acggaacggc ttaccatgac cggcaggccc
     7141 aaagacccca agacagcgcg gaataaaaac gtgctggtga tcggtggcag cggttcaggg
     7201 aaaacgagat ttttcgtgaa acccaacctg atgcaatgct ttccgaccac ggactatccg
     7261 acttcgtttg tggtgacaga tccgaaaggc actttggttc tggaaaccgg caggatgttc
     7321 cagcaggcgg gctaccgcat taaaatcctg aataccatta acttttccaa gtccatgaag
     7381 tacaacccct ttgtctacat ccattcggaa aaggacgtgc taaagctggt aaataccctg
     7441 atttccaaca ccaagggcga gggggaaaag tcagcggagg atttttgggt gaagtcggaa
     7501 cggttgtttt attcggccct gatcggctat atctggtacg aggccccagc ggaggaaatg
     7561 aacttctcaa cgctgctgga aatgataaac gccagtgaag cccgcgagga cgacccggag
     7621 tttcaaagcc ccgtcgatct catgtttgag cgtttggagc agaaagaccc cgaccatttt
     7681 gcggtgcgcc agtacaagaa attcctgctg tccgcaggca agacccgttc ttccatcctg
     7741 atttcatgcg gtgccagact tgcgcccttt gacatccggg aggtacgcga gctgatggag
     7801 gacgacgaaa tggagctgga caccatcggg gacgaaaaga cggtgctgta tctcattatg
     7861 agcgatacgg acacgacctt taatttcatt ctggctatgc tccaaagcca gctaatcaac
     7921 ctgctttgtg accgggctga tgatgtgtac ggcgggcggc tgcctgtcca tgtacgcctg
     7981 attttggatg aatttgccaa cattggccaa atacccaatt tcgataaact cattgccacc
     8041 attcgaagcc gtgaaatatc ggcctctatt attttgcaga gccagtcgca gctaaaagcg
     8101 atttacaagg acgccgccga gatcatttcg gataactgcg atagtgtgct tttccttagt
     8161 gggcgcggca aaaatgccaa ggagatttcc gatgcgctgg ggaaagaaac gattgacagt
     8221 ttcaacacca gcgagaaccg gggaagccaa acgtcccacg gattgaacta tcaaaaatta
     8281 ggaaaggagt tgatgtccca agacgaaatt gccgtgatgg acggcggcaa gtgtatcttg
     8341 cagctacggg gtgtgcgccc gtttttatcg gagaaattcg atattaccaa gcatccccgg
     8401 tacaaatacc ttgccgacgc cgacaagaag aatacctttg acgtggacag atttttgact
     8461 atcacgcgcc ggaaacggca gcaagtggta acgcaggatg aagtattcga cctgtatgaa
     8521 atcgacctgt cggacgagga cgccgccgct gaataaacag cggctttttt cattccaaca
     8581 acaaaatcaa aaaatcatta tgagccggaa accggcagaa agaggtaaat ttatggcatt
     8641 ttttaattcc gcagttacta ctttgcagac tatcgtgatc ggactgggcg gcgctctgtg
     8701 cgtatggggc ggcatcaatc tgttggaggg atacgggcag gacaatccgg gggcaaaatc
     8761 gcagggtgtc aagcagcttg tcgctggcgg cggcgtggcc ctgatcggtg tcactctggt
     8821 tcccctgctg tccgggctgc tgggctgatc ctggcctttg tccctgtcca aacccttaac
     8881 taatcgcctc cgcgtcctcc cggccagacc gggagggcgc gggaaaggag gtgctacccg
     8941 tgattagtga aatcattgaa gaatggataa aaggcatcct catggacggt atcacgggca
     9001 acctgtccgg gctgttcgat acggtaaatg ccaaagtcgg agaaatcgcc gccgacgtgg
     9061 gacgaacccc gcaggcatgg aacagcggta ttttcaatat gctgcacagc ctgtcggaaa
     9121 ccgtgatcgt ccccatagcc ggggccatac tcgcccttgt catgtgctat gagcttatcc
     9181 agatgataac ggaaaagaac aatatgcacg actttgacag ctccatgttc ttccgctgga
     9241 tattcaaatc cgcgtttgca atcctgatcg taacgaacac atggaacatc gtcatgggcg
     9301 tattcgacgc cacccaaagc gtcgtcaatc aaagcgccgg tgtgattatc gggcagacat
     9361 ccattgattt taacaccctg ataccagacc ttgaaagcca attggaggct atgagcgtgg
     9421 gcgctctttt ggggctgtgg ttccaaaccc ttgttgtggg cctcaccatg aatatccttt
     9481 ccatctgcat tttccttgtg acctatggcc gcatggtgga aatctatgtt gtcacgtcgc
     9541 tggggcctat cccccttgcc acaatgggca acgcggaatg gcgcggcatg ggccaagggt
     9601 atctgaaatc cctgctggcg ctgggcttcc aagccttttt aatcatggtt gtggtgggta
     9661 tctacgcggt gctgatacag aatatcggtt ccgcttccga catatccggc gcaatctggg
     9721 gctgtatggg ctatacggtg ctgctgtgct tctgcctgtt caagaccggc agtatatcaa
     9781 aggccgtatt cggggcgcat tgatcccaaa atccaagcat acacaggagg aacttgctta
     9841 tttcaacgta caacatcatt tacgctgacc cgccgtggcg gtatgactgc aaaaacgtac
     9901 agggcgcagc ggaaaatcac tattccacca tgagcattga cgagctgtgc gccctgccgg
     9961 tggaacggct ggcgtccaaa gactgcctgc tgtttttgtg ggccacattc cccatgctgc
    10021 cggaagctct gcggttaatc aaagcgtggg gcttttcatt caagaccgtg gcttttgtat
    10081 ggctgaaaca gaaccgtaaa agccttacat ggttttacgg gctgggccgc tggacaaggg
    10141 gaaacgcgga aatctgcctg ctggcaacac acggcaagcc gaagcgtcgt tctgcctctg
    10201 tccatcagtt tattatcagc ccgattgaac aacacagcaa aaagccggat gtgacccgtg
    10261 aaaaaatcgt agagctggcg ggcgatttgc cccgcgtgga gctgtttgcc agacagaaaa
    10321 cccccggctg ggacgtgtgg gggaacgagg tggaatgtga ccttatcctg acgtgaaagg
    10381 aggtgccaaa tggcttatgt aaccgtcccc aaggacttaa cccatgtgaa aagcaaggtt
    10441 gtgtttgggc ttaccaaacg gcaactgatc tgtttttccg gggcgctggt gatcggcgtc
    10501 cccttatatt ttctcacccg tgactatctt accaacagcg cggcggccct gctcatggta
    10561 tttgccatgc tgccgggctt tttgcttgcc atgtacgagc gtcacggcca gccccttgaa
    10621 gtggtgatta tgcagatcat ccagtgctgc ttcatccagc caaaggagcg tccctatcag
    10681 accgacaaca cctacaccgc ccttgtgcgg caataccaga tggaacagga ggtaaaggcc
    10741 attgtccaaa aaggaaatcc caagagaaac cgaaaaaaca aagctgaccc gcgcccagcg
    10801 aaaagaaatt gacgcggtaa tccgtaaata taagggcgac ggcaggcccc acacggcgca
    10861 gcagagcatc ccctacgagg tcatgtaccc ggacggcgtg tgccgggtat cgcccggcgt
    10921 cttttccaag tgcattgagt ttgcggacat cagctatcag cttgcccagc cggacacgca
    10981 gacggctatc tttgaaaaac tgtgcgacct gtataactat gtggacgcct ccattcatat
    11041 ccagttttct ttcctcaatc ggaaagttga cccggtgcag tacgccaaga gctttgagat
    11101 cgcgccgcag ggggacgact tcgacgacat ccgggccgag tacaccggca ttctgcaaaa
    11161 gcagcttgca aacggcaaca acggcatggt aaagaccaaa tacctgacat tcaccatcga
    11221 ggccgaaagc gtgaaagcgg cgcgagccag attgaagcgc atcggctttg acctgttagg
    11281 ctatttcaag agcatggggg ccgtagccca tgtgatggac ggctgggaac ggctgaacct
    11341 gctgcatggc gtgtaccacc cggacggcga gatattcaat tttgactgga agtggctggc
    11401 cccgtccggc ctctccacca aggattttat tgccccgtcc tccctgtgct tcggcaatgc
    11461 aaagacattc ggcatgggcg gcaaatacgg cgcggtaagt ttcctgcaaa tcctgtcccc
    11521 ggagctgtcg gacgatatgc tggccgactt cctcaatacg gaaagcggtg ttttagtcaa
    11581 tctccatgtg caggccattg aacagaccaa ggcgatcaag accatcaagc gcaaaattac
    11641 cgaccttgac gctatgaaga ttgcggagca gaaaaaggcc gtccgcagcg gttacgatat
    11701 ggacattctt cccagcgacc ttgccaccta cggcgaggac gccaagaagc tgctgacaaa
    11761 gttgcagacc cgcaatgaac ggctgttcca gttgacgttc cttgtcctca acgtggccga
    11821 caccaagcaa aagctgaata acgacgtgtt ccaagccgca ggcgtggccc aaaagcataa
    11881 ctgtcccctt gtccggctgg actaccagca ggaacagggc cttgcaagca gcctgccgct
    11941 gggcgtcaat cagatcaaga tacagcgcag ccttacgact tccagcgtgg ccgtgttcgt
    12001 cccctttgtg acgcaggagc tttttcaggg cggcgcggcc atgtattacg gcatcaacgc
    12061 caagtcccgc aacatgatta tgctggacag gaagcaggcc cgttgcccca atgccttgaa
    12121 gctcggcacc cccggcagcg gcaaatccat gagctgtaaa tccgagatcg tcagtgtgtt
    12181 cttaacgacg cctgacgata tttttatttc agacccggag gccgaatatt acccccttgt
    12241 caaacggctc cacggacagg taatccggct gtcgcctacc agcaaggact ttgtgaaccc
    12301 gctggacatc aacctgaatt attcggagga cgataacccg ctggccctga aatccgactt
    12361 tgtgctgtcg ttctgtgagc ttgtcatggg cggcaagaac gggctggaag ccattgaaaa
    12421 gaccgtgata gaccgggctg tgcgcgtgat ctaccggccc tatctggcag acccccgccc
    12481 ggagaatatg ccgatcctgt ccgacctcca caaagcgttg ttagatcagc atgtcccgga
    12541 ggctgaccgg gtggcgcagg cccttgacct gtatgtgtcc ggctccctga acgtatttaa
    12601 ccaccgtacc aacgtggaca tcggcaaccg gcttgtgtcc tttgacatta aggagctggg
    12661 caagcagctc aaaaagctgg ggatgctcat tgtccaagac cagatatggg ggcgcgtcac
    12721 ggccaaccga agccaaggaa aggccacatg gtattttgcc gacgagttcc atttgctttt
    12781 gaaagaggaa cagacggcgg cctattctgc ggagatttgg aagcggttta ggaaatgggg
    12841 cggcattcca acaggtgcca cccagaacgt caaagacctg ctttcctccc cggaaattga
    12901 aaatatcctt gaaaacagcg acttcattac gctgctcaat caggcgtccg gggatagaaa
    12961 aatccttgcg gaacgcttga atttgtccac cgaacagcag aagtacatcg acaattccga
    13021 acccggcgag ggccttttga tctttgaaaa cgtggtgctg ccctttacca accccatccc
    13081 gcacaacacc cagctttaca agattatgac gacccgctta aacgaggtgg cgggagtatg
    13141 acccggcgac gccacaaagg aggtgaacca ccatgaaaga gctgaaagcc aaggcgaaag
    13201 tgacgcaggc catgacccgc gacgggctga cgctggaaaa tcaggccacc ggcgaggtgg
    13261 agaacatttc cagccgggag gccgaacagg atttgtccgc gccggggccg ggcggcaccg
    13321 ccgataagct gctggaacgg gccgacgcca tccatgacgg ccataaggcc aagaaagccg
    13381 cgaagcaggc cggggctgtg gccgccgagg gggacgctgc cctgcaccgg ccatccgccc
    13441 ggctccaact gtcggaggaa gaacgggccg acccggattt acagccctat atccgaaaat
    13501 cggacaaacg ggccgataag ctggataagg cccgcgccgc catccccaag aagcgcgtcc
    13561 cggtcaagga aaccgtcttt gacgaggcaa gcggcaaagc aaagaccgtc ctgcactttg
    13621 acaaacagga caaggggcct ccctccctga aacccaatcc ggcaggccgt cctgtttccg
    13681 aggtttttct attcggccac gggaaaatcc atgaagtcga gcatgaaaac gtgggtgtgg
    13741 agggcggcca taagggggag gaacttgccg agcggcaggc cggaaaagcc atccgcagca
    13801 ccatccggca ccacaggctg aaaccctact gggacgcgga aaaggccgag cgcaggctat
    13861 tgtctgccaa cgcggagtat ttctaccaga aatctttgcg ggataacccg gagatcgcac
    13921 aggcggccag caaccccatt tcccggttct ggcagaaaca gcagatcaag cgccagtacg
    13981 ccaaggccgc ccggacagcg ggccagaccg caggcgcggc ggcacagggc gcggccaaga
    14041 cagccggggc ctccacgaaa gcggccaagg ccgcagccaa agaaagtgaa cgggcggcct
    14101 cctttgtggc ccgccactgg aaaggggcgc tggccgtcct tgtgttgggg cttttgatgc
    14161 tgcttatcat cggcggctta cagtcctgca ccgccatgtt cggcagcgcc ggaagcggca
    14221 tcacggcgtc ctcttacttt tccgaggata gcgatatgtt ggccgccgag gacgcctacg
    14281 cggagctgga agccgagctg caagactatc tggacaacta cgaggccacc catgactatg
    14341 acgaatacca ttttgacctt gacgagatca gccatgaccc ctatgtgctg atttccattc
    14401 tgtcggcgct ccatgacggg atatttacca tcggggaggt acagggtgat ttgtccatgc
    14461 tctttgacaa gcaatatatc ctgacagaaa acgtcgtggt agaaacccgt taccgcaccg
    14521 aaaccgatac atggacggac gcggagggca acacccatac agacacctac caagtgccgt
    14581 atgactatta catctgctac gtcacgctgg aaaacttcga cctgtcccat gtgccagtct
    14641 atatcatgga cgaggaaacg ctaagtttct attccctgta tatgtccacg ctaggaaacc
    14701 ggcctgatct gttttccggc agccagtacc ccaacgcctc caccctgacg ccgccgacct
    14761 actacgacgt acccccggag gctttggagg atgccacctt tgccgccatg ctcaaagagg
    14821 cggaaaaata tatcggttat ccctatgtgt ggggcggcag ctctcccagc accagctttg
    14881 attgcagcgg cttcatttcg tgggtaatca atcatagtgg atggaacgtg ggacggctgg
    14941 gggcgcaggg cctatataac atctgcaccc ctgtttccag ttcgcaggcc aagcccggcg
    15001 accttgtatt tttcaaggga acctacgaca cccccggcgt gtcccacgtc ggcctgtatg
    15061 tgggcaacaa tgtgatgctg cattgcggga acccgatttc ctacacgaac cttacttcaa
    15121 actactggca atcccatttt tacagttacg ggcgtttacc ttaacgccag aaaggagctt
    15181 tcaattatgg caaccaaact tgaccgcatc gaaaaggaca tccagaaaac caagtctaag
    15241 atcgcggagt ttcagaaaca gcttcgggag ctggaaacgc agaaaaccga acaggagaat
    15301 ttgcagatca tccagcttgt gcgcggcatg aacatgaagc cggaggaatt tgccgcgttc
    15361 ttacgcagcg gcgcaatgca ggccgcccct gccgcaaccc cttaccatga acaggaggac
    15421 aatgcccatg aagaataaga ccagacggat ttcccgtatt ctccccgcac ttctggccgt
    15481 gatgctctgc gtcacggcct ttcccgttac cgcccttgca ggcggcaatg acccggcacc
    15541 ggccccgctg ccggaggcta caaccgaacc cgctacgggc ggtttggagc ctgaaacaga
    15601 cgaaaccggc accgccacgg acgggacaga aaccgaggcc gaaaaagata ccggccctct
    15661 gaccggcaag gacatcaccg acctgttttc cgcgctgttc ggttccaagg tgagcattgc
    15721 cgccacggac gacggcatcc agatcaccac cggcaccgac aaaaaagaac ctgaacagac
    15781 cggcaccgtc accaccaacg gcggcaggct gaatgtccgc accggcgcgg ggcttgacaa
    15841 gaccgcgttc acccagcttc ccaatgggac gacggttgag gtagtcggca cagacggcga
    15901 ctggattaaa attctcttgc cggagcgcat cggctatgtc cattccgact atatgaccgt
    15961 cagcgaaaag gaagtagcgg ctaccggcga gggcggtttt tccctctcca ttgacccgga
    16021 ggaaattgct tctctgctgg aactgttcaa tggcggcggg gccagcgccg ccctgtcccc
    16081 ggacgggaac ctgtcactga ttgacgacat cggcagcgcc gcgaaaaatg gcaagcagtt
    16141 tatcaccgtg gaaaccaaaa acggcaatgt gttttatctg attattgacc gggacgacga
    16201 gggaaaagaa actgtccatt tcttaaatca ggtggatgaa gccgatctgc tgacgctgat
    16261 ggaggacggg gaaaccgtca cccccgctgc tgtctgctcc tgtaccacga aatgcaaggc
    16321 cggggctgtg aacacgaatt gcccggtatg taaaaccaac ctgaccgagt gcagcggccc
    16381 ggagccgcag gaaccccagc cggaggaacc cgaagccccg gaggaagaac ccaagggcgg
    16441 cgcgggcggc ctgatcgtgt tcctgctggt ggcgctggcg ggcggcggtg cggcccttta
    16501 ctatttcaag ttcaaaaagc ccaaggctga aaccaccggc agcgacgatt tgggcgagta
    16561 tgattttggg gaggacgagg atttggagga cgaggaaccc gaaaccgagt ttgacccgga
    16621 agctgatttt gtgagtgaac aggaggacga ggaatgacat ataaacttgt aatcgcggaa
    16681 aaacccagcg tggcccgcag cattgcgggc gtgatcgggg ccacggagaa acacgacggc
    16741 tatttggagg ggaacggcta tctggtgagc tggtgcatcg gacatctggt gtcctttgcc
    16801 gacgccggac gctatgacga gcgtttcaaa aaatggcgct atgaggattt gcccattctc
    16861 ccggaaacat ggcagtacat cgtccccgac gacaagaaac agcagtttga cgttctctgt
    16921 tcgctgatgc agcgccccga cgtgaccggc cttgtgtgcg ccaccgacgc cgggcgcgag
    16981 ggggaattga ttttccgttt cgtctaccag atggcgggat gccaaaagcc ctttgagcgc
    17041 ctttggattt cctctatgga ggacagtgct attaaggacg gctttgccca cctgaaaccg
    17101 ggcggcgact atgatccgct gtatcaatcg gccctgtgcc gggccaaggc cgactggctg
    17161 atcggcatta acgccacccg gcttttttcc gtcctgtacc acaagaacct gactgtgggc
    17221 cgcgtccaga cgccgacctt aaaaatgctg gtggacaggg acgccaagat taccgatttc
    17281 aagaaagaga aatatcacat cgtccatatc acggcgggcg gcgcggacgc ggtaagcagc
    17341 cgcttttccg acgctgccga ggcaaacacc gtcaaggcgg cttgtgcaaa agcgcaggcc
    17401 gtttgcgttt ccgtgacgcg ggagaaaaag acggaacagc cgcccaagct ctatgacctt
    17461 accaccttgc agcgggaggc taaccggctt ttcggctata ccgccaagca gacccttgac
    17521 tatgcccagg cactgtatct aaagaagctg ctgacctatc cgagaacgga tagtcaatat
    17581 cttaccgacg atatgctgcc cgccgtggaa agccttgtgt ccggcctgtg ggagctggtg
    17641 ccgtttgcca agggcctgaa cctttccccg cagtttgacc gcatcctgaa cagcaaaaag
    17701 gtgtccgacc accacgccat tatccccacc gctgaatttg tgaagcaggg ctttggcact
    17761 ctggccgaga gtgaaaaaaa gctgctgtcc ctgatctgct gcaagctctt atgtgccgtg
    17821 gccgggccgc atatctatga ggccgtcacc gccacattta cctgtgccgg gcaggagttt
    17881 accgccaagg gcaagaccgt cctttccccc ggctggaaag agattgaacg ccggttccgt
    17941 tcttccttaa agaccgacgc cgacgaggac gccgaggcgg tgcgggagct gccggagctt
    18001 tgcgagggcc agacctttgc agacgtggcc gcctctgtca ccgagcattt caccacgccg
    18061 cccaagccct acaccgaaga tacgcttttg tccgctatgg aacgggccgg ggccgaggat
    18121 atgccggagg atgccgagcg caagggatta ggcacaccgg ccacccgcgc cgccattctg
    18181 gaaaagctgg tgcagatggg ctttgtccag agaaaaggca agcagctcat tcccaccaag
    18241 gacggcatca atctggctgt ggtgctgccg gaggcgctta cttctcccca gcttactgcc
    18301 gaatgggaaa accggctgac cgagatcgcc aaggggcagg ccgatcctga cgaatttatg
    18361 gacggcatcg agacgcaggc gcgggagctg gtgcagacct attcctgtat cagtgaggaa
    18421 aagcagaaat tgtttcaggc cgagcgtgtt cctatcggga cgtgtccccg ctgtggtgag
    18481 gctgtctatg agggcaagaa aaactactac tgcggaaacc gggcctgcca gttcgtcatg
    18541 tggaagaatg accgattttt tgaggaacgc aagaaagcgt tcaccccgaa gatcgccgct
    18601 gcgctgctca aaaccggcaa agtcaaggta aagggcctct attccgtaaa gaccggcaaa
    18661 acctacgacg ggacggtgct tttggctgat accgggggca agtacgtcaa ctaccgcatt
    18721 gaacacagga gctaaaggac agtaacacag caagcatagg gagtgtggct acactcccgg
    18781 taaaatcccc cggccgcgtc cctgccgggc cggggatttt tgcttgttgg ggaagtcccc
    18841 aaccccctat tttttgaaac agaacagaga aaggagtgac cgcgtgaacg aagtattttc
    18901 tatccaagtc cataaccggc agtttttgga acagggcaaa caaggcgtct ggctggattt
    18961 gcccaccaca accgaaacgc tgcaagccgc cctgcgggat gttggcatta ctgccgacaa
    19021 tccgcaggat ttttttatca acgggtattc ctgcccggag gacagacacc ttgccattcc
    19081 ctatgacatg gtgctggccg ccgacgtgga cgagctgaat ttccttgccg cccggctggg
    19141 ccagcttgac gccgccgaga tcgcagagct gaacgccgcc ctgcaaaatc cccgtggcgg
    19201 ctttgagaat atcggccaga tcatcgacta caccgaaaat gtggattact atgtgcatct
    19261 tcccgacgtt aggggggatg gacagttggg cgactactac ctgaaccgtt ccggcatggt
    19321 ggatatgccg gaggaatgga aagccgggat tgatacggcg cagtttggac gccacattgc
    19381 ccagcaggaa caaggcgttt ttacccagta cggctatctg gtgagaagcg gcgacgaatg
    19441 gcagcgcgtc catgagggcc agccggtgcc ggaggaatac cgggtgatgc actaccccca
    19501 gccggaagct gaccgggagg aaccgcaggc ccagcagccg gaggcatccg aaagccccga
    19561 caaagcgccg cccccggtta tcccgatcat gctggacggc atgaacagca aagaacgcat
    19621 gaaagtgatt actgaccgtc tggaaaccgg cgtacaggag ctttttgaaa gtagccgcta
    19681 tatggcttac ttatccgcta tgtccaagat gcacgattac agtctgaaca atacgctgct
    19741 gatcgccatg cagggcggtt cgattgtggc gggctataac aggtggcagg atgaatttga
    19801 ccgccatgtg aagcggggcg agaaaggcat taagatactg gccccggccc cgttcaaggt
    19861 taagcagcag atggagaaaa tcgacccggc cacccagcag cccgtgatcg gcgcggacgg
    19921 caagcccgtg accgaagaac aggaagtgac ggttccggcc tttaaggtgg tgacagtctt
    19981 tgacatcagc cagaccgagg gcaaggattt tcccatgccc tatatcagtg aacttaccgg
    20041 cgacgtgcag cagtatcagg atttctttgc cgcgctggaa cggacatcac cctatcccat
    20101 aagcattgag ccgatttcag acggggccaa gggccgctgc ttctacgcaa aacagcgcat
    20161 tgccatcaac gagggcatga gcgaattgca gaccctaaag accgccatcc atgagattgc
    20221 ccatgccaag ctccacgcca ttgaccggga ggcccccgcc gaggaacagg cggcccggcc
    20281 agaccgcagc acccgcgagg tacaggccga aagcgtggcc tataccgtct gccagtatta
    20341 cgggctggac acgtcggact attcttttgg ctatgtggcc gattggagca gcggcaggga
    20401 gttatccgag ctgaaagcct cccttgaaac catccgggcc acggccagcg aattgataac
    20461 cgagatcgac ggccattttg cccagcttca gcaggagcgc gaggcccggc aggaacagga
    20521 ggccgtcacg gttgacgtta ccccggaggc agcggccccg gcgcaggccg aggaagaaca
    20581 ggccccggcc cttgatccgg cagccgagcc gcttgtgact gtgctatgga gcgaaagccc
    20641 ccagctaaag gacggccagc aaatgccgtt gcatgaggcc gacgcgctgt ttgcccgtct
    20701 ggatgcagca tatccggcag aggcaggcta tgacaaaacc gcgttccgca ttgactgtat
    20761 ctttcagggc gagccggaca gctacgaggg gcggcaggat tttggagata gggacggttc
    20821 cctgatcgaa catatccaga gctaccacca attttacgcg caggacgaaa actggaaaaa
    20881 gcatgtaatc gctacaaccg gcatagaggg ctgggagcag gacaaggccc atcgggatat
    20941 gctgttaggc gagtttgtcc cctatatgaa gctgcattgc agccttgccc aaatggaaca
    21001 gacggcgcag ggccgcttac agtccgggga aaccctgacg ccggaggaaa cggcctattt
    21061 gggggcgctt ctctcttatg tgcaggagtg ccgccccatg ctcaatcagg gccagtatca
    21121 gttgccggag ccgccgaaac tggctgactt cgaccagagc ttgcaggact acaaagcaca
    21181 ggtacaggcc gagatcgcgc aggaggccgc cgcagccggt atgaccgtgg aggaatacgc
    21241 ggcggctggc tatgaagcac cccaacagga caccttttcc atctaccagc ttcgaggcgg
    21301 ggaggaaacc cgcgactatc ggtttgagcc atacgagcgt ttacaggccg cagggctggc
    21361 cgtggacagt gccaactatg accttgttta tacggcaccg ctggataaaa gcacgtcttt
    21421 ggaggatatt taccgcacat tcaacatcga ccacccggca gattttaggg gacacagcct
    21481 ttccgtttcc gacgtggtgg tgctgcacca gaatggacag gacaccgccc actatgtaga
    21541 cagcttcggc tatatggaag tgccggagtt tttgcgggaa aatcccctgc gtaccgccga
    21601 actgtctacg gagcagaacg aaaacatgat tgacggcatt atcaacaata cgccgcccga
    21661 accgcagccg gaggaacccg aacagctatc ttttgaggat gtggcagttg cttccggcca
    21721 gacacaggag gaacccccgg aggctgcgcc cccgcaaacg gctgtccgct attatcccat
    21781 caatgaggga gccgcccgcc gcgccaagga agccatgagc ttttctgact accggcccgg
    21841 cagcgccacg gcggaatacc ggcactatgt cgatcaggcc gtggaaattg cggagcgcca
    21901 gaaaaagcgt gtggagccag agtatcacga aaagatagac agcctccttg acacctacgc
    21961 ccgcaagctg gccgagaaca tgaataaggg ctatgagatt accgcccgcg tcccctctgt
    22021 cctgattgcc gggccgtcca atttcccggt acgggcgaaa gaaaagcaga acgccgccag
    22081 tgaccgcaac atggaggaat ttcagtacat tcagggcttg cttgacaaaa tccgcagcac
    22141 cggcatgggt ggtatcagcg ccgacgatcc gcaggccgtt tccaagctgg aaaagaaact
    22201 ggaaaagctg gaagcgtcgc aggagttgat gaaggccgtc aatgcctact accgcaagca
    22261 tgggacgctg gacggatgcc cccatctgac cgaaagaggc attgaaaacc tgaaagcgga
    22321 tatggcgtcg ggctggcact atgagaaaaa accgttccag tcctggcagc tctccaacaa
    22381 taacgccgag atacgccggt taaagggccg cattgaggaa ttgacccgcc aaaaggaggc
    22441 ggcctatgtg ggatgggagt ttgacggcgg cactgtggag atcaaccggg tggccaaccg
    22501 cctgcaaatc ttctttgagg ggaagccgga tgctgctgta cgcgacgagc tgaaaagcaa
    22561 cggtttccgc tggtcgccca aggccggggc atggcagcgg cagttaaacg acaccacaat
    22621 ccgcgtcgct gaccgtatca agtgcatcca gcccctttcc ggcgaaaagc ccagcgcctt
    22681 acagcttgcg gcccgcaggg agaaagaaaa gccctctatc cgggcgcagc tcaacgcggc
    22741 caagaccgaa caggccaagc agccaccggc gcgggaaaaa tcaaagggat tggaggtaga
    22801 gtaaatgcag aagtttgaaa atgtggacgt tctggccgcg ctggaacaga tcatgcggca
    22861 gaacaccgcg ttttatcaaa gtgactttgg cattgacaaa gacattatca ggcgggatgc
    22921 cgccagcgat caggccgcag acaagaccct gctctggatg tcccggccct ccgggacgta
    22981 ctgtttccgg gaacgtgatg ttttcctgaa agacacccga cagtacaaca catggaaatt
    23041 ttacggggag cagacccgtg acaagatact ggcctacgcg gtggagctga ccggcacaca
    23101 gggcggcact atccggggca atctctatga gctggactat ccgcagcatt tccggcacgt
    23161 cattgaaacc gcccagccgg tgagtgtgaa ccggctcttt tatgagcatg gcacccgtga
    23221 catcccggag agccagcgtt ttgacggaag ccctgaccgg gtattgggaa actttttgta
    23281 ctatgaggcc cagccccatg acccggctgt gctgcaggag gcgctacgga cggagcagca
    23341 cagccggggg cgggccgtcc ccggcgattt caaagtccac gtcaccgctt tgcatgacag
    23401 ccggattgaa accgaggccc ggcgtattgt ggacagcctg aaagatctgt ccgcgcctaa
    23461 cagccccaat aaaacgcatt tcatggtgga gatctccccg cactttgcgg cgattgcttc
    23521 caccaaggat aatgaccggc tctttgccat gctgccctat aaatccctgt gttttaccgg
    23581 gatgaaaggc aggcatggcg tgtatgccgt gattaaccgg gacgagcgcc gggatgtgag
    23641 tattcgcagg ccccggccct ctatccgcaa gcagctatcc gacagcaaac aggccgccag
    23701 cccaaaaaag gcggcggcca gaaccaaaaa gaatgaattg gaggtataag gaatgatgca
    23761 ctttaccgtg gaggaagaaa acctgatctg tatgtaccac aatgcagacc ggcgcagaac
    23821 gataaacagg ctcaccgccg tcatgctggt tatggatggg gatatgcggg cgcaggccca
    23881 gcagaccctt tccaagctgg aacgcatgac ggatgccgac tatgacggcc agaagttcca
    23941 cttcacagac gaataacgaa cagcagacgc cgggcgcggc ggccatgtgg tcgcttgcgt
    24001 ccggcgtctt ttctttttgc ctattcctac gcatttagaa cccggtattg tcggggatag
    24061 gatgaagtat tcgcttgccc ctgcaaacat gcgggagaag ccttgcagcg ggcggttttc
    24121 aatcccttaa atgcgtaggt tgcagctata attagacccc tgaaacagat atagccgcgc
    24181 agcgggtgta tctgtttcag gggtgtgcag gtgatagccg caaagcggcg taagagggtg
    24241 cagccctctt gccgtctatc tctgttccag cgggaagctc ggcaatgctt ccagcagttt
    24301 cagggaaatg cgctgccgta tatcttcgtc aatgactttc ttctttttgc cgtttgcctg
    24361 tctgatctcc accgtggaaa gctcgttgat gtagtcagca taatgggcca acactttttc
    24421 cgtggcccac ctctcaccgg caacggcggc tttgatcgtt tcatagtcca gcagcttttc
    24481 ttgtccgtcc atcctgatac ccccgtcaga ctgatttttt ctatgatacc atattcggaa
    24541 gcatggaacc agagaaaacg gcgcttttct gctggtaaat tctacgcatt tagaacagca
    24601 cttcggcggg gataggataa aacacccaca taccccgctg accatgccgg aaaagccttg
    24661 aaatataagg gcttttacag ctctaagtgc gtaggtttcc agtatctttt ccgctggcgc
    24721 tcggcctctt tgcggcggcg catccgtttg gagcaggccg cgcagtattt ggcccggtta
    24781 gacttcggcg tgaacgtggc cccgcactca cagcagcgtt tcacttcgtc ccggtctttg
    24841 gtgatctcgg catacagggc cgcatccagc gggaggacag cggccttaaa ccagcggcac
    24901 agaagcgaat aggaaatgct ctgtacgcaa atacattctt cgccgtcgtc cagcaacagg
    24961 cagttgccgt cacaatagtt gcagcactca tgcgtcagct tccgggcctt gcgatattga
    25021 gggtaattca ttcgtggaat actcatagct cttgcgtcca ttcctttccc ggcgtcatac
    25081 gcaaaatgcc gtccacattc tgcttgatga tgccgtattc ctgcatctgc tttttcgctt
    25141 cgccgtactt ctcatacagc cgttcctttt cctcggccaa tcgcttgtac tcggctttca
    25201 gggcgtacag atcggggagc tttttcacac cctgtccctt tagggccttt gccgcagctt
    25261 catacaaaat caacgtgctt tcatgctcac gccggaattt tgccttatcc gctgcattgc
    25321 ggtattccat agccacgggc cagagctgct tgtaggtaga aatattcttt atcagcaccg
    25381 ccatatccgc aagccggtgt tccatagctt tgagggtagc ggcggcctcg tcgctggcgg
    25441 cggtgatctc ggccactttg ctttccaaat ccgggtaggt gtctatccca tgttcagtca
    25501 ggaaattcaa agtctttgcg gcctgcttca aattgtggag cttcgcccac ctctcatagc
    25561 ctgccgactg ctgggcctta atgttgtttt ccagatcaat ccgcaaagaa atccccttgc
    25621 gctctttggg cgctctggtg ataacgcggc ctttgatacg ctctttgatg gcttcctcgg
    25681 tgtaggcttc ccccaatgtc ttgcagcggg taaagcgttc ctgtccggga gcgcggaaag
    25741 aaataaactt tcccggcttg atctcatagc cttgcgcctg catgagccgc aggaaatcat
    25801 caaaatcttt tgcctgcggg atagcggcgt caatggctgc tttgagcttc gctttccagc
    25861 tcgtcccttt ccgctgggcg tcccactcgg catagctctt gcccttactc tggccgggct
    25921 tcaccacgga caatccgttt tccttgcaca gcctgtcgct ggtgcgccgg atataggcat
    25981 agctctgttt gttggaaatg tacttgcgct gatttaccat atccacggca cagaaaataa
    26041 tgtgattgtg gacgtggccc ttatcaatat gggtagtcag cacataggga tatttccctt
    26101 gcagcacttc ctcggcaagc tgcttcccga tctcatgggc ctgttccggc gtggcttcgc
    26161 cgggggcaaa ggattgtatc aggtggtggc ccagattatt gcccttttgg tatgcctgcg
    26221 ccagcagcat ttcaaattca atgtccgctg tttcataaga gcaggcaaag gaggccacca
    26281 gcagcttgcc gtcagttttg tccgggttct cgatgtagtc aagggctttt ttcagcgttg
    26341 acttgatagg atgtatcttt gtaactgcca tatctgcgcc agcgccccct ttatatcctc
    26401 gatgtcctgt gcgtacaccg gccccatacc gttcagcctc tttgcaatct gattgacgtt
    26461 gacgccgatc ttctgtatct cggcggtctg cgcccggatg tcgctggtgt ccatgttgac
    26521 gatatagccg tcaatgagca tcttccgggc gtaggctgaa aagttcttcg tatgcagcag
    26581 cgccattttc tgttcaatca tagcccgttc ctccggcgtc acagggacgc gcagcacgat
    26641 ttttcgcttt ctgtttgcca tgcttcaccg tcctttccat tcaggggttt ggggatttcc
    26701 ccaacaagca aaacggacgc ggcccggcag ggacggggca ggccgttttt ccgggagtgg
    26761 ctacactccc tatgcttgct acgttactgt ttctccacta acaagacgtt tccaaagccc
    26821 caaactaccc gcaaaatagc cctctggaaa gaaaaaagtg caaaaaaata gaagctacgc
    26881 tgtgcagctc ccgctaaaag taaaacagag gatgccagtt acacatcctc cgtttcattc
    26941 ttcttcggct tttcaattag tatggcgctg tcaatcgcgc cctcgatgat cggcaggtat
    27001 tcttcggggc agagtttcag cttgtggctg accctttgcc gcagttcgct ttcctcccgc
    27061 atgatctccg ggttaaagta gcgttccaca ggaagcccgc aggctttaat aagctgtatg
    27121 acaaccggca gactgggaat ggccccgtcg ttttcgatgt tggcaagata gcgccagtca
    27181 atatttattt tctctgccat tgctttgcgc gaaacgcgct ttgcttgccg ggccgctttc
    27241 acatccgcgc cgaaagtttc aaagccggga caatcttcaa tattcgccat atcgcacctc
    27301 ccacttacat tgtatatttc atactctgcc cgtggaatgt tggtatatgc acctttctta
    27361 aagtttatat ttcacatttt gtttctttta ttcttcgagc aaaagaatta taatgtaccc
    27421 tgtggaggtg tactatggac tacatgacgt tgaaagaggc aggcgagaaa tggggtgtgc
    27481 ccccacgatg gataaactac tattgtgctg cgggacgcat ccccggcgct gtaaagatgg
    27541 cgacgatctg gctcattccc aaaactgcgg aaaagccgat tgacaggcgt acaaagaaag
    27601 gaaaggcggt tactgaatag tataatgaaa ccggcgcatt ttggatatgg ttttgaagag
    27661 agagaaagca tggtattttg tcacggaaaa actataagat gatagatggc aggctgctcc
    27721 agaccgataa aaagtattct catctgaaac agaaagaaaa gatttacaca tggatgtatg
    27781 aagaagcgaa actttatcat gacagaaatg gaaaatgccc gaaacaaaga cgaggaagct
    27841 gctgctgtat cagccgtata tggtagaatt gaaaaggccg gcatctggat tctttatggt
    27901 gaagtctcca gacattggat aactgctggt ctaatgcgcc tttgagtatg gggtgattga
    27961 ttttaatacc ggggaggtac ggctttttga ggaaaatatt ctgggattta catttggcga
    28021 ttattacatt tcctgtaact gcaaggacac ggatgatgaa gatatgagga atatagactt
    28081 gctgattttc tactgtcttg aacggtatta ggactgaatc tgctgcgcgg agtgcggcaa
    28141 gagcgaccgg agggagtaca cacaggccct gcctgtgtga aaagccatac cgcaaggtgc
    28201 attcgcaaaa aagaggccgc gcattttgct gaggcttggg gtgtccccaa caagcaaaac
    28261 gagaacgccg caggcgggca agtggtggat cagaatatcc gtaaaggata ttctgatcca
    28321 ttgcttgcca gtattatcgg gatgttttga caaccgctgt aatcgaaaca tcccaaacat
    28381 taagaaggtg ttaattatta ttatcttgat ttaattcgtt taaaccaata atatagatac
    28441 atacaccatg aaaagaagcg agtatcccaa tgacggatga ttaaaatccg aaaagaaatc
    28501 gctttgaaac tacagggaaa ctacagactg ttatgttatc ttcttaaaat ggagggattt
    28561 ttatgtcgat acgaattcta cttgtcgagg atgatgatca tatctgtaat acagtaaggg
    28621 cgtttctggc tgaggcagga tatcaggtgg atgcctgcac agatggaaat gaggcataca
    28681 ccaagtttta cgaaaacact tatcaactgg ttattcttga tattatgctg cccggtatga
    28741 acgggcatga acttttgcgt gaatttcgtg cgaaaaatga tactcccatt ctgatgatga
    28801 cagccctgac ggatgacgaa aaccaaatcc gggcgtttga tgcagaggca gacgactatg
    28861 taacaaagcc attcaagatg cagattttac taaagcgggt ggaagccctg ctgcggcgca
    28921 gcggtgcgct ggcaaaggaa atccgtgtcg gcaggctgac acttctgccg gaggatttca
    28981 cggtactttg tgacggtaca gagctgcccc tgacacgaaa agaatttgaa atccttttgc
    29041 tgctggtgca gaacaaaggc agaaccttaa cgcatgaaat cattttgtca cgtatatggg
    29101 gatatgactt tgagggtgat ggcagcacag tccacactca tatcaaaaat ttgcgggcca
    29161 agctgccgga aaatatcatc aaaaccattc gcggtgtagg ataccgattg gaggaatcat
    29221 aataatggaa agaaaaggga ttttcattaa ggttttttcc tatacgatca ttgccctgct
    29281 actgcttgtc ggtgtaacag caacactgtt tgcacagcaa tttgtgtctt atttcagagt
    29341 gatggaatta cagcaaacag taaaatccta tcagccattg gtggaactga ttcagaatag
    29401 cgataggctt gatattcaag aggtggcagg gctgtttcac tacaataacc aatcctttga
    29461 gttttatatt gaagataaag agggaagcgt actctatgcc acaccaaatg ccaatacatc
    29521 aaatagtttt agacccgact ttctttatgt ggtacataga gatgataata tttcgattgt
    29581 tgctcaaagc aaggcaggtg tgggattgct ttatcaaggg ctgacaattc ggggaattgt
    29641 tatgattgcg ataatggttg tattcagcct tttatgcgcg tatatctttg cgcggcaaat
    29701 gacaacgccg atcaaagcct tagcggacag tgcgaataaa atggcaaacc tgaaagatgt
    29761 accgccgccg ctggagcgaa aggatgagct tggcgcactg gctcatg
//
LOCUS       DANKHL010000406        29807 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.guided.006, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000406 DANKHL010000000
VERSION     DANKHL010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.guided.006"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(39..1439)
                     /locus_tag="PW101_002987"
     CDS             complement(39..1439)
                     /locus_tag="PW101_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HDL2515327.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            1657..3318
                     /locus_tag="PW101_002988"
     CDS             1657..3318
                     /locus_tag="PW101_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDL2515328.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            3275..3973
                     /locus_tag="PW101_002989"
     CDS             3275..3973
                     /locus_tag="PW101_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL2515329.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            3989..4510
                     /locus_tag="PW101_002990"
                     /pseudo
     CDS             3989..4510
                     /locus_tag="PW101_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            4767..4955
                     /locus_tag="PW101_002991"
                     /pseudo
     CDS             4767..4955
                     /locus_tag="PW101_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            5001..5966
                     /locus_tag="PW101_002992"
     CDS             5001..5966
                     /locus_tag="PW101_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2515330.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            5988..6200
                     /locus_tag="PW101_002993"
     CDS             5988..6200
                     /locus_tag="PW101_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDL2515331.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            6275..6751
                     /locus_tag="PW101_002994"
     CDS             6275..6751
                     /locus_tag="PW101_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HDL2515332.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            6748..8556
                     /locus_tag="PW101_002995"
     CDS             6748..8556
                     /locus_tag="PW101_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDL2515333.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            8633..8848
                     /locus_tag="PW101_002996"
     CDS             8633..8848
                     /locus_tag="PW101_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HDL2515334.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            8940..9803
                     /locus_tag="PW101_002997"
     CDS             8940..9803
                     /locus_tag="PW101_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515335.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            9840..10376
                     /locus_tag="PW101_002998"
     CDS             9840..10376
                     /locus_tag="PW101_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HDL2515336.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            10390..10812
                     /locus_tag="PW101_002999"
     CDS             10390..10812
                     /locus_tag="PW101_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HDL2515337.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            10742..13141
                     /locus_tag="PW101_003000"
     CDS             10742..13141
                     /locus_tag="PW101_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HDL2515338.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            13173..15164
                     /locus_tag="PW101_003001"
     CDS             13173..15164
                     /locus_tag="PW101_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2515339.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            15187..15438
                     /locus_tag="PW101_003002"
     CDS             15187..15438
                     /locus_tag="PW101_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HDL2515340.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            15428..16657
                     /locus_tag="PW101_003003"
     CDS             15428..16657
                     /locus_tag="PW101_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HDL2515341.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            16654..18735
                     /locus_tag="PW101_003004"
     CDS             16654..18735
                     /locus_tag="PW101_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HDL2515342.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            18884..22804
                     /locus_tag="PW101_003005"
     CDS             18884..22804
                     /locus_tag="PW101_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HDL2515343.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            22805..23749
                     /locus_tag="PW101_003006"
     CDS             22805..23749
                     /locus_tag="PW101_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515344.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            23753..23956
                     /locus_tag="PW101_003007"
     CDS             23753..23956
                     /locus_tag="PW101_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HDL2515345.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMLVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(24256..24492)
                     /locus_tag="PW101_003008"
     CDS             complement(24256..24492)
                     /locus_tag="PW101_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2515346.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(24675..25061)
                     /locus_tag="PW101_003009"
     CDS             complement(24675..25061)
                     /locus_tag="PW101_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HDL2515347.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(25043..26371)
                     /locus_tag="PW101_003010"
     CDS             complement(25043..26371)
                     /locus_tag="PW101_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL2515348.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(26332..26661)
                     /gene="mobC"
                     /locus_tag="PW101_003011"
     CDS             complement(26332..26661)
                     /gene="mobC"
                     /locus_tag="PW101_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HDL2515349.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(26919..27290)
                     /locus_tag="PW101_003012"
     CDS             complement(26919..27290)
                     /locus_tag="PW101_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2515350.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            27435..27620
                     /locus_tag="PW101_003013"
     CDS             27435..27620
                     /locus_tag="PW101_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL2515351.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            27778..27945
                     /locus_tag="PW101_003014"
     CDS             27778..27945
                     /locus_tag="PW101_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515352.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            28132..28350
                     /locus_tag="PW101_003015"
     CDS             28132..28350
                     /locus_tag="PW101_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515353.1"
                     /translation="MRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            28563..29222
                     /gene="vanR"
                     /locus_tag="PW101_003016"
     CDS             28563..29222
                     /gene="vanR"
                     /locus_tag="PW101_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HDL2515354.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            29225..>29807
                     /locus_tag="PW101_003017"
     CDS             29225..>29807
                     /locus_tag="PW101_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDL2515355.1"
                     /translation="MERKGIFIKVFSYTIIALLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAH"
BASE COUNT         7707 a         7632 c         7937 g         6531 t
ORIGIN      
        1 cttatttctt gaagctgacc acttctgtca caacctcttt aagaatgatg gatagaaata
       61 cgtttaggat aagttttttt agatgcactt tgttcgattt cctctaaaga tttcccacga
      121 gtttctggta cgatctcttg aataaataaa acccctaaaa agcagatgat cccaaaaatg
      181 ccgaaaacga tttcttgaga catagaagca gtcatcacag gaaaaagcaa gccaactagg
      241 aaagaaccaa tccaattaaa agaagaggct aggccagatg ctcgcccgcg gacagctagt
      301 gggaatattt cgcctatgat tacccatgtc agtggagccc atgtgcaaga ataaaatgcg
      361 acataaatac ataaaaacaa gagaatcaac aacgaatttg tatgagcatc cagtacagtt
      421 cctaaaacag ctggtaggat gaatgaaagt cccatcacgc taccgcctat tttcaataaa
      481 gttcgccgat tgaacttgtc tgcaataacc aaaaagagca aagaaccagc aactaaaatg
      541 attccttgga tgatcggcca catcaatgca tcgctcgctg catgcccagt cgctttttct
      601 acgatcagcg gaatatagta aaaaatagca ttagctcctt gaaattgctg gaatactgct
      661 acaccaatac cggcaatgac taaggaacga tatttttcta aaaacaaagt atgccaagaa
      721 gttccggttg tcttttcttg tttgacattc agctggattt gttcgaattc ctgttcagct
      781 tcttttggtg tccgaatata gttcaatact ctttttgcct cttccaagcg gccagattgc
      841 ataagaaaac gcggtgactc tggtagagct aaaacaccga aaaataaaat cagcgcgggg
      901 acagcagcga gccctaacat cacgcgccaa gccatcgttt caggcaacac ttttagcaaa
      961 taatctgcaa cataggaaag gagcatgcca gaagcaatca tcacttgatt gatccctgac
     1021 aatcttcccc ttagtctagc aggagccatt tcagacatat aagcaggaac tagggcagat
     1081 gcagcgccaa cagctaaacc tagaagaatc cttgctgcaa tcagaaaata agaaccattg
     1141 tgaggggcag atgcagaaag gacagaacca gccataaaaa taagtgcaga aagaagaatc
     1201 atttttcgtc gtccaagctt atcagaaaga agaccagata aagagccgcc aaggatcgca
     1261 cctaacatga ccgaagacgt gatccatcct atcaaagaag ctttacctgc caaaccccaa
     1321 tcatgctgta aaaaaggtaa ggcgccggtc ataacaccaa tatcatatcc aaatagaatg
     1381 ccgccaaatg cgccaaaaaa ataaataaac gatgaggaaa cttttttttc ttttttcata
     1441 agtattctct ccttctagaa tttcgagaag gcggttgatc agattttgta agcaatttca
     1501 ttttataact tatgtacgta caaaacaagt gttttttatt gtattcttgt aaataaccgc
     1561 ttgacatgta cgtacaaaag tatacaataa tcattgtaag cgctactaaa aagacggttt
     1621 gatcaggttt gacgagaagg agggagttaa atcattatga aggaagtaaa tgcgcaaagt
     1681 atgttagatg tcatagatgg tcgtacatct ttagggattg agtttggatc aactaggatc
     1741 aaagctgtat tgattgattc atcttttact cctattgccg ctggtagtta tgagtgggaa
     1801 aacaaactag aaaatggtat ctggacctat tcattagaag atatttggaa aggactgcgc
     1861 gtcagttacc gaaaaatggc agcagaagta ttcgaaatct atggcgagaa tcttacgaca
     1921 atcgggtcga ttgggttcag tgcaatgatg cacggttatc tagcatttga tagcaaggaa
     1981 cagttactag taccatttcg tacttggcga aatacaatca ctgaagaagc agaacgaata
     2041 ttaacggaaa aatttcaata caatattccg cagagatgga gtatcgctca tttgtatcaa
     2101 tctattttga ataaagaaga acatgtgaaa gaaattaatt ttttgacaac gcttgcaggt
     2161 tatgtccatt ggcagcttac tggtgagaaa gtactaggcg taggagatgc atcaggcatg
     2221 tttcctatcg atccttcaac gaaaacctac catcaagaaa tgaaaaatat ttttggtcag
     2281 ttaatcgaaa aagcgcacgt agcgattgac ttggatcaca ttttgcctaa agttctagta
     2341 gcaggagaag aagctggcaa gttaagcgaa tcaggggctt atctgatcga ccctacggga
     2401 aatttgagac caggaatccc ggtttgtccg ccagaagggg atgcaggaac cggtatggtc
     2461 gctacgaaca gtgtagccaa acggacaggg aacatttcgg ctggtacttc cgccttcgct
     2521 atgatcgtat tagaaaaaga actagaaaat gtccatccgg aaatcgacat ggtcactaca
     2581 ccttcaggag aactagtagg catggttcat acgaataact gctcttcgga tatcaatgct
     2641 tgggtcagta tttttgaaga atttaccaat tctttgggta tggagataag cagagataaa
     2701 ttattcacgg tattattcaa caaagcgcta gaaggcgata cggattgcgg gggattactt
     2761 tcttatggtt atttttccgg tgaaaacatt acaggagtaa atgaaggaag gccattattt
     2821 gtacgtacac caggtagccg ttttgattta gcgaatttca tgcggatcca tttagccagt
     2881 gcttttggtg caatgagaat cggaatggat atattgaaat ctgagaatgt ccaaattgat
     2941 cgtttagtgg ggcatggggg gatttttaaa acaccagaag ttggacagcg gattctagca
     3001 tctgctatgg aggcaccagt tacagtgatg gacacagccg gagaaggtgg agcgtgggga
     3061 atcgcgttat tagcagccta tatgatggat cgacatggga agtctttaga aaacttcttg
     3121 gcagaagacg tgtttggaca agaccaagga atcaccatca caccttcagc agaagaaata
     3181 gcaggctatc agatatttat gagacgttac cgtgagggga tcgacattga aaaaacagcg
     3241 attcatcaat tgcaagaaaa aggagtgata gaaaatgctg gaacaactga aagaagaagt
     3301 ataccaagca aatcttgact tgcctaaaca cggactcgtc aaatatacat ggggaaatgt
     3361 gagcgctttt gatcctgaaa cacgatattt cgttatcaaa cctagcggag tcagttatga
     3421 agagatgaca gcagacgaca tggtcgttgt ggacttggac aatcatatca ttgaaggtaa
     3481 actcaatccc tcttcggata cgcctactca tgctgtttta tatcgttcat ttcctcaaat
     3541 cggaggaatc gtccatactc actcgacatg ggcgacgatc tgggcacaag caggattgga
     3601 tgttcctgcg atggggacga cgcatgcaga tactttttat ggatcggtac cttgtgctcg
     3661 atttttgaca caacaggaaa tcgacagcgg gtacgaatat gaaacaggaa aagttatcat
     3721 agaaacattc aaagaacgca aaattgatcc cttagcaatt ccaggggtac ttttacacgg
     3781 acacggtcca tttacttggg ggaaagatgc gcaaagtgcg gtaatgaacg cagtcgtact
     3841 agatgaagta tgcaagatga atttatttac cagacagctc aattcatttt cagaagagct
     3901 gcctcaacgg attttagata aacattattt acgaaaacac ggaaaaaatg cgtattacgg
     3961 acaaaacaaa taaaaggagt ggctaattat gttgaatatt ggagaaaaag aattttggtt
     4021 tgttgtagga tcacagcatt tatatgggga agaagcactg cgagaagtaa agaaacatgc
     4081 acaagaaatg gtcgatgaac tgaatgaaaa tggccaattg ccttatccaa tccgccttca
     4141 agaattagca gttactgctg atacgatcac aaaaatcatg aaagaggtca attatcgaga
     4201 agaagtagca ggtgtgatca cttggatgca tactttctca ccagcaaaaa tgtggatccg
     4261 cgggacaaag cttctacaaa aaccattgct tcatttagct acgcaatata acgaaagcat
     4321 tccttggaaa acgatcgata tggatttcat gaatttaaat caatccgctc atggtgaccg
     4381 cgaatatggg ttcatcaatg ctcgattaaa taaacaaaat aaaatcgtcg ttggctactg
     4441 gaaacgacct gagatccaaa aagagattgc tgattggatg gatgttgcag tagcctataa
     4501 tgaaagtttt attccaaaaa tttaggttat cgctgaaagc cccggaaata cgggcatttc
     4561 gcaggatatg agaactcaat ttactaccaa tttactactt tcggattgag ccgcatatat
     4621 gctgaaccat actgctaata cccgccaccc caaatacggc acatcataaa ttgaatacga
     4681 caccgtaaac gcggcgttcc tctttcccgt catgggagaa caggacgccg cttttttatg
     4741 cccaaataga gaaaggaggc gacgccatgc tatttacttc cggccctgac cgggcgtttg
     4801 aacggctgat gcaggaaaag cccggtttcg atcactacga cggcggcggg gcggacgcgc
     4861 cggaggattg cggcacctgc cgattttacc gcccggattg gaaatatcaa ttctgccagt
     4921 ttgcagagtg tccctatcag ccgggcaagc tgaccgacta tgcagcggtc aaattcacag
     4981 tgaaaggagc gaacaacgct atggcagttt tccgtgtgga aaagaacaaa ggctatacgg
     5041 tgatgtcaaa tcaccacctg cggaataaga acctgacctt aaaggccaag ggcctgctgt
     5101 cgcagatgtt gtccttgccg gaggattggg actataccct aaaaggactg tcctatatca
     5161 accgggaaag catcgacgcg atcaggacag cggtatggga gcttgaaaaa gctggatata
     5221 tccggcgtga gcagggccgt gacgcaaaag gcaaaatggc cgatatggtc tataccatct
     5281 acgaacagcc ggtattggaa aacccggtat tggaaaatcc aacatcggat aacccggtgt
     5341 tggaaaatcc gacatcggat aatccggcgt cggaaaatcc aacgcaatta aataaagaag
     5401 tacaaagaac tgacttatca aaaaaagaaa aaataattac agatggacaa agtacccatt
     5461 ccattcctat cccatccccg acccctgccc ctatggaggg cgaggcagcg gctacgccgc
     5521 cggaacggaa aaggacagga aaggatgagg ctgtacggat ttatcgggaa atcattttgg
     5581 agaacatcga gtatgcctat ctcattcagg acaggagcat tgaccgtgaa cagcttgacg
     5641 aaatcgtaga cctgatgctg gaaaccgtct gcaccgcccg aaagacaatc cgtgtcgccg
     5701 gggacgacta ccctgccgag ctggtgaaat ccaagtacat gaagctgacc ggcgaacata
     5761 tccgctttgt ccttgactgt ctgcgggaga acacaacgaa agtccgcaat atcaaacaat
     5821 acctgcgggc tatgctgttc aacgccccca gcaccatttc caactactac acggcccttg
     5881 tcgcgcacga catggcacag cccgattggg gaaagcctaa atccggcctg ccggactatt
     5941 cctgcggcga gggcgaaagc ctgtaacgag aaaaggagga cgaccctatg aagcagggcg
     6001 cattgatttt tgacgagcga acagaccgtt acgacatccg ctttgatctg gccgactatt
     6061 acggcggcct gcattgcggg gagtgtttcg acgtgatgat cggcggcaaa tggaggccca
     6121 cccgcattga aatggccgaa aactggtatc ttgtcggcat ccgggccgac gacctttccg
     6181 gcctgcgggt gcggatttga cctatatcta cctgcggcgt ctggtggttc cgccttagac
     6241 gccgcttttg actttctgac aaggagggat gcgattgcag gaagaagtta ctcaaaagac
     6301 cattgccctg tccatgaaaa cgggcaagct cacggctcaa gtgctgcaag cggcgctgaa
     6361 aaagtacctg caacaccggg ccaagggcaa aaacacgctg caccacggcc agcagagcct
     6421 aaagcagctt aaaaagcatg gggctgatct ctccaacatt gagatcaccg aggccaacat
     6481 tggagcgttc aagccatgcg ccaagaaata cggcgtggat tttaccttac gaaaagataa
     6541 gaccacccag ccgccccact acgttgtgat tttcaaggcc aaggatgcag ataaccttga
     6601 tctggcgttt cgggagttta ccgccaagac gctttccaag gagcagcggc cctctatccg
     6661 caaggttttg tccgctgtga agcaaaaggc ggcggcccag accaagcagc gggccaagga
     6721 gaaaatcaag gaaagggggc tggaactgtg aagcctgacg taaagaaatt gctgatcctg
     6781 aacctcccgt atctgatttt tgtgtacctg tttgcgaaat gcggggaggc gtaccggctg
     6841 gccgctggtg ctgacctgtc ggggaagctc ctgcattttg cggatggttt tgcggcggct
     6901 tttgccaacc cgctgccaag cctgcacctg tttgacctgt gtatcggtat cgccggtgcc
     6961 gtgatcgtgc ggctggtggt gtactgcaag agcaagaacg tgaagaaata ccgcaaaggc
     7021 atggagtatg gcagcgcccg ctgggggacg gccaaagaca tagcgcctta catcgacccc
     7081 aagtttgaaa ataatatcct gctcacccaa acggaacggc ttaccatgac cggcaggccc
     7141 aaagacccca agacagcgcg gaataaaaac gtgctggtga tcggtggcag cggttcaggg
     7201 aaaacgagat ttttcgtgaa acccaacctg atgcaatgct ttccgaccac ggactatccg
     7261 acttcgtttg tggtgacaga tccgaaaggc actttggttc tggaaaccgg caggatgttc
     7321 cagcaggcgg gctaccgcat taaaatcctg aataccatta acttttccaa gtccatgaag
     7381 tacaacccct ttgtctacat ccattcggaa aaggacgtgc taaagctggt aaataccctg
     7441 atttccaaca ccaagggcga gggggaaaag tcagcggagg atttttgggt gaagtcggaa
     7501 cggttgtttt attcggccct gatcggctat atctggtacg aggccccagc ggaggaaatg
     7561 aacttctcaa cgctgctgga aatgataaac gccagtgaag cccgcgagga cgacccggag
     7621 tttcaaagcc ccgtcgatct catgtttgag cgtttggagc agaaagaccc cgaccatttt
     7681 gcggtgcgcc agtacaagaa attcctgctg tccgcaggca agacccgttc ttccatcctg
     7741 atttcatgcg gtgccagact tgcgcccttt gacatccggg aggtacgcga gctgatggag
     7801 gacgacgaaa tggagctgga caccatcggg gacgaaaaga cggtgctgta tctcattatg
     7861 agcgatacgg acacgacctt taatttcatt ctggctatgc tccaaagcca gctaatcaac
     7921 ctgctttgtg accgggctga tgatgtgtac ggcgggcggc tgcctgtcca tgtacgcctg
     7981 attttggatg aatttgccaa cattggccaa atacccaatt tcgataaact cattgccacc
     8041 attcgaagcc gtgaaatatc ggcctctatt attttgcaga gccagtcgca gctaaaagcg
     8101 atttacaagg acgccgccga gatcatttcg gataactgcg atagtgtgct tttccttagt
     8161 gggcgcggca aaaatgccaa ggagatttcc gatgcgctgg ggaaagaaac gattgacagt
     8221 ttcaacacca gcgagaaccg gggaagccaa acgtcccacg gattgaacta tcaaaaatta
     8281 ggaaaggagt tgatgtccca agacgaaatt gccgtgatgg acggcggcaa gtgtatcttg
     8341 cagctacggg gtgtgcgccc gtttttatcg gagaaattcg atattaccaa gcatccccgg
     8401 tacaaatacc ttgccgacgc cgacaagaag aatacctttg acgtggacag atttttgact
     8461 atcacgcgcc ggaaacggca gcaagtggta acgcaggatg aagtattcga cctgtatgaa
     8521 atcgacctgt cggacgagga cgccgccgct gaataaacag cggctttttt cattccaaca
     8581 acaaaatcaa aaaatcatta tgagccggaa accggcagaa agaggtaaat ttatggcatt
     8641 ttttaattcc gcagttacta ctttgcagac tatcgtgatc ggactgggcg gcgctctgtg
     8701 cgtatggggc ggcatcaatc tgttggaggg atacgggcag gacaatccgg gggcaaaatc
     8761 gcagggtgtc aagcagcttg tcgctggcgg cggcgtggcc ctgatcggtg tcactctggt
     8821 tcccctgctg tccgggctgc tgggctgatc ctggcctttg tccctgtcca aacccttaac
     8881 taatcgcctc cgcgtcctcc cggccagacc gggagggcgc gggaaaggag gtgctacccg
     8941 tgattagtga aatcattgaa gaatggataa aaggcatcct catggacggt atcacgggca
     9001 acctgtccgg gctgttcgat acggtaaatg ccaaagtcgg agaaatcgcc gccgacgtgg
     9061 gacgaacccc gcaggcatgg aacagcggta ttttcaatat gctgcacagc ctgtcggaaa
     9121 ccgtgatcgt ccccatagcc ggggccatac tcgcccttgt catgtgctat gagcttatcc
     9181 agatgataac ggaaaagaac aatatgcacg actttgacag ctccatgttc ttccgctgga
     9241 tattcaaatc cgcgtttgca atcctgatcg taacgaacac atggaacatc gtcatgggcg
     9301 tattcgacgc cacccaaagc gtcgtcaatc aaagcgccgg tgtgattatc gggcagacat
     9361 ccattgattt taacaccctg ataccagacc ttgaaagcca attggaggct atgagcgtgg
     9421 gcgctctttt ggggctgtgg ttccaaaccc ttgttgtggg cctcaccatg aatatccttt
     9481 ccatctgcat tttccttgtg acctatggcc gcatggtgga aatctatgtt gtcacgtcgc
     9541 tggggcctat cccccttgcc acaatgggca acgcggaatg gcgcggcatg ggccaagggt
     9601 atctgaaatc cctgctggcg ctgggcttcc aagccttttt aatcatggtt gtggtgggta
     9661 tctacgcggt gctgatacag aatatcggtt ccgcttccga catatccggc gcaatctggg
     9721 gctgtatggg ctatacggtg ctgctgtgct tctgcctgtt caagaccggc agtatatcaa
     9781 aggccgtatt cggggcgcat tgatcccaaa atccaagcat acacaggagg aacttgctta
     9841 tttcaacgta caacatcatt tacgctgacc cgccgtggcg gtatgactgc aaaaacgtac
     9901 agggcgcagc ggaaaatcac tattccacca tgagcattga cgagctgtgc gccctgccgg
     9961 tggaacggct ggcgtccaaa gactgcctgc tgtttttgtg ggccacattc cccatgctgc
    10021 cggaagctct gcggttaatc aaagcgtggg gcttttcatt caagaccgtg gcttttgtat
    10081 ggctgaaaca gaaccgtaaa agccttacat ggttttacgg gctgggccgc tggacaaggg
    10141 gaaacgcgga aatctgcctg ctggcaacac acggcaagcc gaagcgtcgt tctgcctctg
    10201 tccatcagtt tattatcagc ccgattgaac aacacagcaa aaagccggat gtgacccgtg
    10261 aaaaaatcgt agagctggcg ggcgatttgc cccgcgtgga gctgtttgcc agacagaaaa
    10321 cccccggctg ggacgtgtgg gggaacgagg tggaatgtga ccttatcctg acgtgaaagg
    10381 aggtgccaaa tggcttatgt aaccgtcccc aaggacttaa cccatgtgaa aagcaaggtt
    10441 gtgtttgggc ttaccaaacg gcaactgatc tgtttttccg gggcgctggt gatcggcgtc
    10501 cccttatatt ttctcacccg tgactatctt accaacagcg cggcggccct gctcatggta
    10561 tttgccatgc tgccgggctt tttgcttgcc atgtacgagc gtcacggcca gccccttgaa
    10621 gtggtgatta tgcagatcat ccagtgctgc ttcatccagc caaaggagcg tccctatcag
    10681 accgacaaca cctacaccgc ccttgtgcgg caataccaga tggaacagga ggtaaaggcc
    10741 attgtccaaa aaggaaatcc caagagaaac cgaaaaaaca aagctgaccc gcgcccagcg
    10801 aaaagaaatt gacgcggtaa tccgtaaata taagggcgac ggcaggcccc acacggcgca
    10861 gcagagcatc ccctacgagg tcatgtaccc ggacggcgtg tgccgggtat cgcccggcgt
    10921 cttttccaag tgcattgagt ttgcggacat cagctatcag cttgcccagc cggacacgca
    10981 gacggctatc tttgaaaaac tgtgcgacct gtataactat gtggacgcct ccattcatat
    11041 ccagttttct ttcctcaatc ggaaagttga cccggtgcag tacgccaaga gctttgagat
    11101 cgcgccgcag ggggacgact tcgacgacat ccgggccgag tacaccggca ttctgcaaaa
    11161 gcagcttgca aacggcaaca acggcatggt aaagaccaaa tacctgacat tcaccatcga
    11221 ggccgaaagc gtgaaagcgg cgcgagccag attgaagcgc atcggctttg acctgttagg
    11281 ctatttcaag agcatggggg ccgtagccca tgtgatggac ggctgggaac ggctgaacct
    11341 gctgcatggc gtgtaccacc cggacggcga gatattcaat tttgactgga agtggctggc
    11401 cccgtccggc ctctccacca aggattttat tgccccgtcc tccctgtgct tcggcaatgc
    11461 aaagacattc ggcatgggcg gcaaatacgg cgcggtaagt ttcctgcaaa tcctgtcccc
    11521 ggagctgtcg gacgatatgc tggccgactt cctcaatacg gaaagcggtg ttttagtcaa
    11581 tctccatgtg caggccattg aacagaccaa ggcgatcaag accatcaagc gcaaaattac
    11641 cgaccttgac gctatgaaga ttgcggagca gaaaaaggcc gtccgcagcg gttacgatat
    11701 ggacattctt cccagcgacc ttgccaccta cggcgaggac gccaagaagc tgctgacaaa
    11761 gttgcagacc cgcaatgaac ggctgttcca gttgacgttc cttgtcctca acgtggccga
    11821 caccaagcaa aagctgaata acgacgtgtt ccaagccgca ggcgtggccc aaaagcataa
    11881 ctgtcccctt gtccggctgg actaccagca ggaacagggc cttgcaagca gcctgccgct
    11941 gggcgtcaat cagatcaaga tacagcgcag ccttacgact tccagcgtgg ccgtgttcgt
    12001 cccctttgtg acgcaggagc tttttcaggg cggcgcggcc atgtattacg gcatcaacgc
    12061 caagtcccgc aacatgatta tgctggacag gaagcaggcc cgttgcccca atgccttgaa
    12121 gctcggcacc cccggcagcg gcaaatccat gagctgtaaa tccgagatcg tcagtgtgtt
    12181 cttaacgacg cctgacgata tttttatttc agacccggag gccgaatatt acccccttgt
    12241 caaacggctc cacggacagg taatccggct gtcgcctacc agcaaggact ttgtgaaccc
    12301 gctggacatc aacctgaatt attcggagga cgataacccg ctggccctga aatccgactt
    12361 tgtgctgtcg ttctgtgagc ttgtcatggg cggcaagaac gggctggaag ccattgaaaa
    12421 gaccgtgata gaccgggctg tgcgcgtgat ctaccggccc tatctggcag acccccgccc
    12481 ggagaatatg ccgatcctgt ccgacctcca caaagcgttg ttagatcagc atgtcccgga
    12541 ggctgaccgg gtggcgcagg cccttgacct gtatgtgtcc ggctccctga acgtatttaa
    12601 ccaccgtacc aacgtggaca tcggcaaccg gcttgtgtcc tttgacatta aggagctggg
    12661 caagcagctc aaaaagctgg ggatgctcat tgtccaagac cagatatggg ggcgcgtcac
    12721 ggccaaccga agccaaggaa aggccacatg gtattttgcc gacgagttcc atttgctttt
    12781 gaaagaggaa cagacggcgg cctattctgc ggagatttgg aagcggttta ggaaatgggg
    12841 cggcattcca acaggtgcca cccagaacgt caaagacctg ctttcctccc cggaaattga
    12901 aaatatcctt gaaaacagcg acttcattac gctgctcaat caggcgtccg gggatagaaa
    12961 aatccttgcg gaacgcttga atttgtccac cgaacagcag aagtacatcg acaattccga
    13021 acccggcgag ggccttttga tctttgaaaa cgtggtgctg ccctttacca accccatccc
    13081 gcacaacacc cagctttaca agattatgac gacccgctta aacgaggtgg cgggagtatg
    13141 acccggcgac gccacaaagg aggtgaacca ccatgaaaga gctgaaagcc aaggcgaaag
    13201 tgacgcaggc catgacccgc gacgggctga cgctggaaaa tcaggccacc ggcgaggtgg
    13261 agaacatttc cagccgggag gccgaacagg atttgtccgc gccggggccg ggcggcaccg
    13321 ccgataagct gctggaacgg gccgacgcca tccatgacgg ccataaggcc aagaaagccg
    13381 cgaagcaggc cggggctgtg gccgccgagg gggacgctgc cctgcaccgg ccatccgccc
    13441 ggctccaact gtcggaggaa gaacgggccg acccggattt acagccctat atccgaaaat
    13501 cggacaaacg ggccgataag ctggataagg cccgcgccgc catccccaag aagcgcgtcc
    13561 cggtcaagga aaccgtcttt gacgaggcaa gcggcaaagc aaagaccgtc ctgcactttg
    13621 acaaacagga caaggggcct ccctccctga aacccaatcc ggcaggccgt cctgtttccg
    13681 aggtttttct attcggccac gggaaaatcc atgaagtcga gcatgaaaac gtgggtgtgg
    13741 agggcggcca taagggggag gaacttgccg agcggcaggc cggaaaagcc atccgcagca
    13801 ccatccggca ccacaggctg aaaccctact gggacgcgga aaaggccgag cgcaggctat
    13861 tgtctgccaa cgcggagtat ttctaccaga aatctttgcg ggataacccg gagatcgcac
    13921 aggcggccag caaccccatt tcccggttct ggcagaaaca gcagatcaag cgccagtacg
    13981 ccaaggccgc ccggacagcg ggccagaccg caggcgcggc ggcacagggc gcggccaaga
    14041 cagccggggc ctccacgaaa gcggccaagg ccgcagccaa agaaagtgaa cgggcggcct
    14101 cctttgtggc ccgccactgg aaaggggcgc tggccgtcct tgtgttgggg cttttgatgc
    14161 tgcttatcat cggcggctta cagtcctgca ccgccatgtt cggcagcgcc ggaagcggca
    14221 tcacggcgtc ctcttacttt tccgaggata gcgatatgtt ggccgccgag gacgcctacg
    14281 cggagctgga agccgagctg caagactatc tggacaacta cgaggccacc catgactatg
    14341 acgaatacca ttttgacctt gacgagatca gccatgaccc ctatgtgctg atttccattc
    14401 tgtcggcgct ccatgacggg atatttacca tcggggaggt acagggtgat ttgtccatgc
    14461 tctttgacaa gcaatatatc ctgacagaaa acgtcgtggt agaaacccgt taccgcaccg
    14521 aaaccgatac atggacggac gcggagggca acacccatac agacacctac caagtgccgt
    14581 atgactatta catctgctac gtcacgctgg aaaacttcga cctgtcccat gtgccagtct
    14641 atatcatgga cgaggaaacg ctaagtttct attccctgta tatgtccacg ctaggaaacc
    14701 ggcctgatct gttttccggc agccagtacc ccaacgcctc caccctgacg ccgccgacct
    14761 actacgacgt acccccggag gctttggagg atgccacctt tgccgccatg ctcaaagagg
    14821 cggaaaaata tatcggttat ccctatgtgt ggggcggcag ctctcccagc accagctttg
    14881 attgcagcgg cttcatttcg tgggtaatca atcatagtgg atggaacgtg ggacggctgg
    14941 gggcgcaggg cctatataac atctgcaccc ctgtttccag ttcgcaggcc aagcccggcg
    15001 accttgtatt tttcaaggga acctacgaca cccccggcgt gtcccacgtc ggcctgtatg
    15061 tgggcaacaa tgtgatgctg cattgcggga acccgatttc ctacacgaac cttacttcaa
    15121 actactggca atcccatttt tacagttacg ggcgtttacc ttaacgccag aaaggagctt
    15181 tcaattatgg caaccaaact tgaccgcatc gaaaaggaca tccagaaaac caagtctaag
    15241 atcgcggagt ttcagaaaca gcttcgggag ctggaaacgc agaaaaccga acaggagaat
    15301 ttgcagatca tccagcttgt gcgcggcatg aacatgaagc cggaggaatt tgccgcgttc
    15361 ttacgcagcg gcgcaatgca ggccgcccct gccgcaaccc cttaccatga acaggaggac
    15421 aatgcccatg aagaataaga ccagacggat ttcccgtatt ctccccgcac ttctggccgt
    15481 gatgctctgc gtcacggcct ttcccgttac cgcccttgca ggcggcaatg acccggcacc
    15541 ggccccgctg ccggaggcta caaccgaacc cgctacgggc ggtttggagc ctgaaacaga
    15601 cgaaaccggc accgccacgg acgggacaga aaccgaggcc gaaaaagata ccggccctct
    15661 gaccggcaag gacatcaccg acctgttttc cgcgctgttc ggttccaagg tgagcattgc
    15721 cgccacggac gacggcatcc agatcaccac cggcaccgac aaaaaagaac ctgaacagac
    15781 cggcaccgtc accaccaacg gcggcaggct gaatgtccgc accggcgcgg ggcttgacaa
    15841 gaccgcgttc acccagcttc ccaatgggac gacggttgag gtagtcggca cagacggcga
    15901 ctggattaaa attctcttgc cggagcgcat cggctatgtc cattccgact atatgaccgt
    15961 cagcgaaaag gaagtagcgg ctaccggcga gggcggtttt tccctctcca ttgacccgga
    16021 ggaaattgct tctctgctgg aactgttcaa tggcggcggg gccagcgccg ccctgtcccc
    16081 ggacgggaac ctgtcactga ttgacgacat cggcagcgcc gcgaaaaatg gcaagcagtt
    16141 tatcaccgtg gaaaccaaaa acggcaatgt gttttatctg attattgacc gggacgacga
    16201 gggaaaagaa actgtccatt tcttaaatca ggtggatgaa gccgatctgc tgacgctgat
    16261 ggaggacggg gaaaccgtca cccccgctgc tgtctgctcc tgtaccacga aatgcaaggc
    16321 cggggctgtg aacacgaatt gcccggtatg taaaaccaac ctgaccgagt gcagcggccc
    16381 ggagccgcag gaaccccagc cggaggaacc cgaagccccg gaggaagaac ccaagggcgg
    16441 cgcgggcggc ctgatcgtgt tcctgctggt ggcgctggcg ggcggcggtg cggcccttta
    16501 ctatttcaag ttcaaaaagc ccaaggctga aaccaccggc agcgacgatt tgggcgagta
    16561 tgattttggg gaggacgagg atttggagga cgaggaaccc gaaaccgagt ttgacccgga
    16621 agctgatttt gtgagtgaac aggaggacga ggaatgacat ataaacttgt aatcgcggaa
    16681 aaacccagcg tggcccgcag cattgcgggc gtgatcgggg ccacggagaa acacgacggc
    16741 tatttggagg ggaacggcta tctggtgagc tggtgcatcg gacatctggt gtcctttgcc
    16801 gacgccggac gctatgacga gcgtttcaaa aaatggcgct atgaggattt gcccattctc
    16861 ccggaaacat ggcagtacat cgtccccgac gacaagaaac agcagtttga cgttctctgt
    16921 tcgctgatgc agcgccccga cgtgaccggc cttgtgtgcg ccaccgacgc cgggcgcgag
    16981 ggggaattga ttttccgttt cgtctaccag atggcgggat gccaaaagcc ctttgagcgc
    17041 ctttggattt cctctatgga ggacagtgct attaaggacg gctttgccca cctgaaaccg
    17101 ggcggcgact atgatccgct gtatcaatcg gccctgtgcc gggccaaggc cgactggctg
    17161 atcggcatta acgccacccg gcttttttcc gtcctgtacc acaagaacct gactgtgggc
    17221 cgcgtccaga cgccgacctt aaaaatgctg gtggacaggg acgccaagat taccgatttc
    17281 aagaaagaga aatatcacat cgtccatatc acggcgggcg gcgcggacgc ggtaagcagc
    17341 cgcttttccg acgctgccga ggcaaacacc gtcaaggcgg cttgtgcaaa agcgcaggcc
    17401 gtttgcgttt ccgtgacgcg ggagaaaaag acggaacagc cgcccaagct ctatgacctt
    17461 accaccttgc agcgggaggc taaccggctt ttcggctata ccgccaagca gacccttgac
    17521 tatgcccagg cactgtatct aaagaagctg ctgacctatc cgagaacgga tagtcaatat
    17581 cttaccgacg atatgctgcc cgccgtggaa agccttgtgt ccggcctgtg ggagctggtg
    17641 ccgtttgcca agggcctgaa cctttccccg cagtttgacc gcatcctgaa cagcaaaaag
    17701 gtgtccgacc accacgccat tatccccacc gctgaatttg tgaagcaggg ctttggcact
    17761 ctggccgaga gtgaaaaaaa gctgctgtcc ctgatctgct gcaagctctt atgtgccgtg
    17821 gccgggccgc atatctatga ggccgtcacc gccacattta cctgtgccgg gcaggagttt
    17881 accgccaagg gcaagaccgt cctttccccc ggctggaaag agattgaacg ccggttccgt
    17941 tcttccttaa agaccgacgc cgacgaggac gccgaggcgg tgcgggagct gccggagctt
    18001 tgcgagggcc agacctttgc agacgtggcc gcctctgtca ccgagcattt caccacgccg
    18061 cccaagccct acaccgaaga tacgcttttg tccgctatgg aacgggccgg ggccgaggat
    18121 atgccggagg atgccgagcg caagggatta ggcacaccgg ccacccgcgc cgccattctg
    18181 gaaaagctgg tgcagatggg ctttgtccag agaaaaggca agcagctcat tcccaccaag
    18241 gacggcatca atctggctgt ggtgctgccg gaggcgctta cttctcccca gcttactgcc
    18301 gaatgggaaa accggctgac cgagatcgcc aaggggcagg ccgatcctga cgaatttatg
    18361 gacggcatcg agacgcaggc gcgggagctg gtgcagacct attcctgtat cagtgaggaa
    18421 aagcagaaat tgtttcaggc cgagcgtgtt cctatcggga cgtgtccccg ctgtggtgag
    18481 gctgtctatg agggcaagaa aaactactac tgcggaaacc gggcctgcca gttcgtcatg
    18541 tggaagaatg accgattttt tgaggaacgc aagaaagcgt tcaccccgaa gatcgccgct
    18601 gcgctgctca aaaccggcaa agtcaaggta aagggcctct attccgtaaa gaccggcaaa
    18661 acctacgacg ggacggtgct tttggctgat accgggggca agtacgtcaa ctaccgcatt
    18721 gaacacagga gctaaaggac agtaacacag caagcatagg gagtgtggct acactcccgg
    18781 taaaatcccc cggccgcgtc cctgccgggc cggggatttt tgcttgttgg ggaagtcccc
    18841 aaccccctat tttttgaaac agaacagaga aaggagtgac cgcgtgaacg aagtattttc
    18901 tatccaagtc cataaccggc agtttttgga acagggcaaa caaggcgtct ggctggattt
    18961 gcccaccaca accgaaacgc tgcaagccgc cctgcgggat gttggcatta ctgccgacaa
    19021 tccgcaggat ttttttatca acgggtattc ctgcccggag gacagacacc ttgccattcc
    19081 ctatgacatg gtgctggccg ccgacgtgga cgagctgaat ttccttgccg cccggctggg
    19141 ccagcttgac gccgccgaga tcgcagagct gaacgccgcc ctgcaaaatc cccgtggcgg
    19201 ctttgagaat atcggccaga tcatcgacta caccgaaaat gtggattact atgtgcatct
    19261 tcccgacgtt aggggggatg gacagttggg cgactactac ctgaaccgtt ccggcatggt
    19321 ggatatgccg gaggaatgga aagccgggat tgatacggcg cagtttggac gccacattgc
    19381 ccagcaggaa caaggcgttt ttacccagta cggctatctg gtgagaagcg gcgacgaatg
    19441 gcagcgcgtc catgagggcc agccggtgcc ggaggaatac cgggtgatgc actaccccca
    19501 gccggaagct gaccgggagg aaccgcaggc ccagcagccg gaggcatccg aaagccccga
    19561 caaagcgccg cccccggtta tcccgatcat gctggacggc atgaacagca aagaacgcat
    19621 gaaagtgatt actgaccgtc tggaaaccgg cgtacaggag ctttttgaaa gtagccgcta
    19681 tatggcttac ttatccgcta tgtccaagat gcacgattac agtctgaaca atacgctgct
    19741 gatcgccatg cagggcggtt cgattgtggc gggctataac aggtggcagg atgaatttga
    19801 ccgccatgtg aagcggggcg agaaaggcat taagatactg gccccggccc cgttcaaggt
    19861 taagcagcag atggagaaaa tcgacccggc cacccagcag cccgtgatcg gcgcggacgg
    19921 caagcccgtg accgaagaac aggaagtgac ggttccggcc tttaaggtgg tgacagtctt
    19981 tgacatcagc cagaccgagg gcaaggattt tcccatgccc tatatcagtg aacttaccgg
    20041 cgacgtgcag cagtatcagg atttctttgc cgcgctggaa cggacatcac cctatcccat
    20101 aagcattgag ccgatttcag acggggccaa gggccgctgc ttctacgcaa aacagcgcat
    20161 tgccatcaac gagggcatga gcgaattgca gaccctaaag accgccatcc atgagattgc
    20221 ccatgccaag ctccacgcca ttgaccggga ggcccccgcc gaggaacagg cggcccggcc
    20281 agaccgcagc acccgcgagg tacaggccga aagcgtggcc tataccgtct gccagtatta
    20341 cgggctggac acgtcggact attcttttgg ctatgtggcc gattggagca gcggcaggga
    20401 gttatccgag ctgaaagcct cccttgaaac catccgggcc acggccagcg aattgataac
    20461 cgagatcgac ggccattttg cccagcttca gcaggagcgc gaggcccggc aggaacagga
    20521 ggccgtcacg gttgacgtta ccccggaggc agcggccccg gcgcaggccg aggaagaaca
    20581 ggccccggcc cttgatccgg cagccgagcc gcttgtgact gtgctatgga gcgaaagccc
    20641 ccagctaaag gacggccagc aaatgccgtt gcatgaggcc gacgcgctgt ttgcccgtct
    20701 ggatgcagca tatccggcag aggcaggcta tgacaaaacc gcgttccgca ttgactgtat
    20761 ctttcagggc gagccggaca gctacgaggg gcggcaggat tttggagata gggacggttc
    20821 cctgatcgaa catatccaga gctaccacca attttacgcg caggacgaaa actggaaaaa
    20881 gcatgtaatc gctacaaccg gcatagaggg ctgggagcag gacaaggccc atcgggatat
    20941 gctgttaggc gagtttgtcc cctatatgaa gctgcattgc agccttgccc aaatggaaca
    21001 gacggcgcag ggccgcttac agtccgggga aaccctgacg ccggaggaaa cggcctattt
    21061 gggggcgctt ctctcttatg tgcaggagtg ccgccccatg ctcaatcagg gccagtatca
    21121 gttgccggag ccgccgaaac tggctgactt cgaccagagc ttgcaggact acaaagcaca
    21181 ggtacaggcc gagatcgcgc aggaggccgc cgcagccggt atgaccgtgg aggaatacgc
    21241 ggcggctggc tatgaagcac cccaacagga caccttttcc atctaccagc ttcgaggcgg
    21301 ggaggaaacc cgcgactatc ggtttgagcc atacgagcgt ttacaggccg cagggctggc
    21361 cgtggacagt gccaactatg accttgttta tacggcaccg ctggataaaa gcacgtcttt
    21421 ggaggatatt taccgcacat tcaacatcga ccacccggca gattttaggg gacacagcct
    21481 ttccgtttcc gacgtggtgg tgctgcacca gaatggacag gacaccgccc actatgtaga
    21541 cagcttcggc tatatggaag tgccggagtt tttgcgggaa aatcccctgc gtaccgccga
    21601 actgtctacg gagcagaacg aaaacatgat tgacggcatt atcaacaata cgccgcccga
    21661 accgcagccg gaggaacccg aacagctatc ttttgaggat gtggcagttg cttccggcca
    21721 gacacaggag gaacccccgg aggctgcgcc cccgcaaacg gctgtccgct attatcccat
    21781 caatgaggga gccgcccgcc gcgccaagga agccatgagc ttttctgact accggcccgg
    21841 cagcgccacg gcggaatacc ggcactatgt cgatcaggcc gtggaaattg cggagcgcca
    21901 gaaaaagcgt gtggagccag agtatcacga aaagatagac agcctccttg acacctacgc
    21961 ccgcaagctg gccgagaaca tgaataaggg ctatgagatt accgcccgcg tcccctctgt
    22021 cctgattgcc gggccgtcca atttcccggt acgggcgaaa gaaaagcaga acgccgccag
    22081 tgaccgcaac atggaggaat ttcagtacat tcagggcttg cttgacaaaa tccgcagcac
    22141 cggcatgggt ggtatcagcg ccgacgatcc gcaggccgtt tccaagctgg aaaagaaact
    22201 ggaaaagctg gaagcgtcgc aggagttgat gaaggccgtc aatgcctact accgcaagca
    22261 tgggacgctg gacggatgcc cccatctgac cgaaagaggc attgaaaacc tgaaagcgga
    22321 tatggcgtcg ggctggcact atgagaaaaa accgttccag tcctggcagc tctccaacaa
    22381 taacgccgag atacgccggt taaagggccg cattgaggaa ttgacccgcc aaaaggaggc
    22441 ggcctatgtg ggatgggagt ttgacggcgg cactgtggag atcaaccggg tggccaaccg
    22501 cctgcaaatc ttctttgagg ggaagccgga tgctgctgta cgcgacgagc tgaaaagcaa
    22561 cggtttccgc tggtcgccca aggccggggc atggcagcgg cagttaaacg acaccacaat
    22621 ccgcgtcgct gaccgtatca agtgcatcca gcccctttcc ggcgaaaagc ccagcgcctt
    22681 acagcttgcg gcccgcaggg agaaagaaaa gccctctatc cgggcgcagc tcaacgcggc
    22741 caagaccgaa caggccaagc agccaccggc gcgggaaaaa tcaaagggat tggaggtaga
    22801 gtaaatgcag aagtttgaaa atgtggacgt tctggccgcg ctggaacaga tcatgcggca
    22861 gaacaccgcg ttttatcaaa gtgactttgg cattgacaaa gacattatca ggcgggatgc
    22921 cgccagcgat caggccgcag acaagaccct gctctggatg tcccggccct ccgggacgta
    22981 ctgtttccgg gaacgtgatg ttttcctgaa agacacccga cagtacaaca catggaaatt
    23041 ttacggggag cagacccgtg acaagatact ggcctacgcg gtggagctga ccggcacaca
    23101 gggcggcact atccggggca atctctatga gctggactat ccgcagcatt tccggcacgt
    23161 cattgaaacc gcccagccgg tgagtgtgaa ccggctcttt tatgagcatg gcacccgtga
    23221 catcccggag agccagcgtt ttgacggaag ccctgaccgg gtattgggaa actttttgta
    23281 ctatgaggcc cagccccatg acccggctgt gctgcaggag gcgctacgga cggagcagca
    23341 cagccggggg cgggccgtcc ccggcgattt caaagtccac gtcaccgctt tgcatgacag
    23401 ccggattgaa accgaggccc ggcgtattgt ggacagcctg aaagatctgt ccgcgcctaa
    23461 cagccccaat aaaacgcatt tcatggtgga gatctccccg cactttgcgg cgattgcttc
    23521 caccaaggat aatgaccggc tctttgccat gctgccctat aaatccctgt gttttaccgg
    23581 gatgaaaggc aggcatggcg tgtatgccgt gattaaccgg gacgagcgcc gggatgtgag
    23641 tattcgcagg ccccggccct ctatccgcaa gcagctatcc gacagcaaac aggccgccag
    23701 cccaaaaaag gcggcggcca gaaccaaaaa gaatgaattg gaggtataag gaatgatgca
    23761 ctttaccgtg gaggaagaaa acctgatctg tatgtaccac aatgcagacc ggcgcagaac
    23821 gataaacagg ctcaccgccg tcatgctggt tatggatggg gatatgcggg cgcaggccca
    23881 gcagaccctt tccaagctgg aacgcatgac ggatgccgac tatgacggcc agaagttcca
    23941 cttcacagac gaataacgaa cagcagacgc cgggcgcggc ggccatgtgg tcgcttgcgt
    24001 ccggcgtctt ttctttttgc ctattcctac gcatttagaa cccggtattg tcggggatag
    24061 gatgaagtat tcgcttgccc ctgcaaacat gcgggagaag ccttgcagcg ggcggttttc
    24121 aatcccttaa atgcgtaggt tgcagctata attagacccc tgaaacagat atagccgcgc
    24181 agcgggtgta tctgtttcag gggtgtgcag gtgatagccg caaagcggcg taagagggtg
    24241 cagccctctt gccgtctatc tctgttccag cgggaagctc ggcaatgctt ccagcagttt
    24301 cagggaaatg cgctgccgta tatcttcgtc aatgactttc ttctttttgc cgtttgcctg
    24361 tctgatctcc accgtggaaa gctcgttgat gtagtcagca taatgggcca acactttttc
    24421 cgtggcccac ctctcaccgg caacggcggc tttgatcgtt tcatagtcca gcagcttttc
    24481 ttgtccgtcc atcctgatac ccccgtcaga ctgatttttt ctatgatacc atattcggaa
    24541 gcatggaacc agagaaaacg gcgcttttct gctggtaaat tctacgcatt tagaacagca
    24601 cttcggcggg gataggataa aacacccaca taccccgctg accatgccgg aaaagccttg
    24661 aaatataagg gcttttacag ctctaagtgc gtaggtttcc agtatctttt ccgctggcgc
    24721 tcggcctctt tgcggcggcg catccgtttg gagcaggccg cgcagtattt ggcccggtta
    24781 gacttcggcg tgaacgtggc cccgcactca cagcagcgtt tcacttcgtc ccggtctttg
    24841 gtgatctcgg catacagggc cgcatccagc gggaggacag cggccttaaa ccagcggcac
    24901 agaagcgaat aggaaatgct ctgtacgcaa atacattctt cgccgtcgtc cagcaacagg
    24961 cagttgccgt cacaatagtt gcagcactca tgcgtcagct tccgggcctt gcgatattga
    25021 gggtaattca ttcgtggaat actcatagct cttgcgtcca ttcctttccc ggcgtcatac
    25081 gcaaaatgcc gtccacattc tgcttgatga tgccgtattc ctgcatctgc tttttcgctt
    25141 cgccgtactt ctcatacagc cgttcctttt cctcggccaa tcgcttgtac tcggctttca
    25201 gggcgtacag atcggggagc tttttcacac cctgtccctt tagggccttt gccgcagctt
    25261 catacaaaat caacgtgctt tcatgctcac gccggaattt tgccttatcc gctgcattgc
    25321 ggtattccat agccacgggc cagagctgct tgtaggtaga aatattcttt atcagcaccg
    25381 ccatatccgc aagccggtgt tccatagctt tgagggtagc ggcggcctcg tcgctggcgg
    25441 cggtgatctc ggccactttg ctttccaaat ccgggtaggt gtctatccca tgttcagtca
    25501 ggaaattcaa agtctttgcg gcctgcttca aattgtggag cttcgcccac ctctcatagc
    25561 ctgccgactg ctgggcctta atgttgtttt ccagatcaat ccgcaaagaa atccccttgc
    25621 gctctttggg cgctctggtg ataacgcggc ctttgatacg ctctttgatg gcttcctcgg
    25681 tgtaggcttc ccccaatgtc ttgcagcggg taaagcgttc ctgtccggga gcgcggaaag
    25741 aaataaactt tcccggcttg atctcatagc cttgcgcctg catgagccgc aggaaatcat
    25801 caaaatcttt tgcctgcggg atagcggcgt caatggctgc tttgagcttc gctttccagc
    25861 tcgtcccttt ccgctgggcg tcccactcgg catagctctt gcccttactc tggccgggct
    25921 tcaccacgga caatccgttt tccttgcaca gcctgtcgct ggtgcgccgg atataggcat
    25981 agctctgttt gttggaaatg tacttgcgct gatttaccat atccacggca cagaaaataa
    26041 tgtgattgtg gacgtggccc ttatcaatat gggtagtcag cacataggga tatttccctt
    26101 gcagcacttc ctcggcaagc tgcttcccga tctcatgggc ctgttccggc gtggcttcgc
    26161 cgggggcaaa ggattgtatc aggtggtggc ccagattatt gcccttttgg tatgcctgcg
    26221 ccagcagcat ttcaaattca atgtccgctg tttcataaga gcaggcaaag gaggccacca
    26281 gcagcttgcc gtcagttttg tccgggttct cgatgtagtc aagggctttt ttcagcgttg
    26341 acttgatagg atgtatcttt gtaactgcca tatctgcgcc agcgccccct ttatatcctc
    26401 gatgtcctgt gcgtacaccg gccccatacc gttcagcctc tttgcaatct gattgacgtt
    26461 gacgccgatc ttctgtatct cggcggtctg cgcccggatg tcgctggtgt ccatgttgac
    26521 gatatagccg tcaatgagca tcttccgggc gtaggctgaa aagttcttcg tatgcagcag
    26581 cgccattttc tgttcaatca tagcccgttc ctccggcgtc acagggacgc gcagcacgat
    26641 ttttcgcttt ctgtttgcca tgcttcaccg tcctttccat tcaggggttt ggggatttcc
    26701 ccaacaagca aaacggacgc ggcccggcag ggacggggca ggccgttttt ccgggagtgg
    26761 ctacactccc tatgcttgct acgttactgt ttctccacta acaagacgtt tccaaagccc
    26821 caaactaccc gcaaaatagc cctctggaaa gaaaaaagtg caaaaaaata gaagctacgc
    26881 tgtgcagctc ccgctaaaag taaaacagag gatgccagtt acacatcctc cgtttcattc
    26941 ttcttcggct tttcaattag tatggcgctg tcaatcgcgc cctcgatgat cggcaggtat
    27001 tcttcggggc agagtttcag cttgtggctg accctttgcc gcagttcgct ttcctcccgc
    27061 atgatctccg ggttaaagta gcgttccaca ggaagcccgc aggctttaat aagctgtatg
    27121 acaaccggca gactgggaat ggccccgtcg ttttcgatgt tggcaagata gcgccagtca
    27181 atatttattt tctctgccat tgctttgcgc gaaacgcgct ttgcttgccg ggccgctttc
    27241 acatccgcgc cgaaagtttc aaagccggga caatcttcaa tattcgccat atcgcacctc
    27301 ccacttacat tgtatatttc atactctgcc cgtggaatgt tggtatatgc acctttctta
    27361 aagtttatat ttcacatttt gtttctttta ttcttcgagc aaaagaatta taatgtaccc
    27421 tgtggaggtg tactatggac tacatgacgt tgaaagaggc aggcgagaaa tggggtgtgc
    27481 ccccacgatg gataaactac tattgtgctg cgggacgcat ccccggcgct gtaaagatgg
    27541 cgacgatctg gctcattccc aaaactgcgg aaaagccgat tgacaggcgt acaaagaaag
    27601 gaaaggcggt tactgaatag tataatgaaa ccggcgcatt ttggatatgg ttttgaagag
    27661 agagaaagca tggtattttg tcacggaaaa actataagat gatagatggc aggctgctcc
    27721 agaccgataa aaagtattct catctgaaac agaaagaaaa gatttacaca tggatgtatg
    27781 aagaagcgaa actttatcat gacagaaatg gaaaatgccc gaaacaaaga cgaggaagct
    27841 gctgctgtat cagccgtata tggtagaatt gaaaaggccg gcatctggat tctttatggt
    27901 gaagtctcca gacattggat aactgctggt ctaatgcgcc tttgagtatg gggtgattga
    27961 ttttaatacc ggggaggtac ggctttttga ggaaaatatt ctgggattta catttggcga
    28021 ttattacatt tcctgtaact gcaaggacac ggatgatgaa gatatgagga atatagactt
    28081 gctgattttc tactgtcttg aacggtatta ggactgaatc tgctgcgcgg agtgcggcaa
    28141 gagcgaccgg agggagtaca cacaggccct gcctgtgtga aaagccatac cgcaaggtgc
    28201 attcgcaaaa aagaggccgc gcattttgct gaggcttggg gtgtccccaa caagcaaaac
    28261 gagaacgccg caggcgggca agtggtggat cagaatatcc gtaaaggata ttctgatcca
    28321 ttgcttgcca gtattatcgg gatgttttga caaccgctgt aatcgaaaca tcccaaacat
    28381 taagaaggtg ttaattatta ttatcttgat ttaattcgtt taaaccaata atatagatac
    28441 atacaccatg aaaagaagcg agtatcccaa tgacggatga ttaaaatccg aaaagaaatc
    28501 gctttgaaac tacagggaaa ctacagactg ttatgttatc ttcttaaaat ggagggattt
    28561 ttatgtcgat acgaattcta cttgtcgagg atgatgatca tatctgtaat acagtaaggg
    28621 cgtttctggc tgaggcagga tatcaggtgg atgcctgcac agatggaaat gaggcataca
    28681 ccaagtttta cgaaaacact tatcaactgg ttattcttga tattatgctg cccggtatga
    28741 acgggcatga acttttgcgt gaatttcgtg cgaaaaatga tactcccatt ctgatgatga
    28801 cagccctgtc ggatgacgaa aaccaaatcc gggcgtttga tgcagaggca gacgactatg
    28861 taacaaagcc attcaagatg cagattttac taaagcgggt ggaagccctg ctgcggcgca
    28921 gcggtgcgct ggcaaaggaa atccgtgtcg gcaggctgac acttctgccg gaggatttca
    28981 cggtactttg tgacggtaca gagctgcccc tgacacgaaa agaatttgaa atccttttgc
    29041 tgctggtgca gaacaaaggc agaaccttaa cgcatgaaat cattttgtca cgtatatggg
    29101 gatatgactt tgagggtgat ggcagcacag tccacactca tatcaaaaat ttgcgggcca
    29161 agctgccgga aaatatcatc aaaaccattc gcggtgtagg ataccgattg gaggaatcat
    29221 aataatggaa agaaaaggga ttttcattaa ggttttttcc tatacgatca ttgccctgct
    29281 actgcttgtc ggtgtaacag caacactgtt tgcacagcaa tttgtgtctt atttcagagt
    29341 gatggaatta cagcaaacag taaaatccta tcagccattg gtggaactga ttcagaatag
    29401 cgataggctt gatattcaag aggtggcagg gctgtttcac tacaataacc aatcctttga
    29461 gttttatatt gaagataaag agggaagcgt actctatgcc acaccaaatg ccaatacatc
    29521 aaatagtttt agacccgact ttctttatgt ggtacataga gatgataata tttcgattgt
    29581 tgctcaaagc aaggcaggtg tgggattgct ttatcaaggg ctgacaattc ggggaattgt
    29641 tatgattgcg ataatggttg tattcagcct tttatgcgcg tatatctttg cgcggcaaat
    29701 gacaacgccg atcaaagcct tagcggacag tgcgaataaa atggcaaacc tgaaagatgt
    29761 accgccgccg ctggagcgaa aggatgagct tggcgcactg gctcatg
//
LOCUS       DANKHL010000407        15167 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.guided.057, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000407 DANKHL010000000
VERSION     DANKHL010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.guided.057"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            complement(134..577)
                     /locus_tag="PW101_003018"
     CDS             complement(134..577)
                     /locus_tag="PW101_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDL2515356.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="PW101_003019"
     CDS             complement(795..2135)
                     /locus_tag="PW101_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2515357.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="PW101_003020"
     CDS             2404..3300
                     /locus_tag="PW101_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDL2515358.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="PW101_003021"
     CDS             complement(3450..4391)
                     /locus_tag="PW101_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL2515359.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="PW101_003022"
     CDS             4517..5638
                     /locus_tag="PW101_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL2515360.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="PW101_003023"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="PW101_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL2515361.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="PW101_003024"
     CDS             7426..7992
                     /locus_tag="PW101_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2515362.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="PW101_003025"
     CDS             8203..9264
                     /locus_tag="PW101_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL2515363.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="PW101_003026"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="PW101_003026"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL2515364.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="PW101_003027"
     CDS             10177..12159
                     /locus_tag="PW101_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2515365.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="PW101_003028"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="PW101_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDL2515366.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="PW101_003029"
     CDS             12881..14305
                     /locus_tag="PW101_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2515367.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..>15167)
                     /locus_tag="PW101_003030"
     CDS             complement(14486..>15167)
                     /locus_tag="PW101_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2515368.1"
                     /translation="TALLAKKYTTIIDPVEMTAQLFSATSWIVFIMIAAASTIISMEA
                     QYGTLKNLLYRKYSRGEILVSKWITLVIYSVYLYLLAIIVTVLMKLILFPSISFTEKV
                     STGQTLIQSLFTYTLGSYIGLWLILSLVLMIACFINSSGASISAGIVFYFASSIISGI
                     LIALIQKWEWIKWNPISMLNLQNQIGNEEIMKQLTHLSTNQMLFGNLAYIVLFLALGY
                     LVFKRKNV"
BASE COUNT         4913 a         2741 c         3172 g         4341 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataaaaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgtt
//
LOCUS       DANKHL010000408        10568 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.guided.100, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000408 DANKHL010000000
VERSION     DANKHL010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.guided.100"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..512
                     /locus_tag="PW101_003031"
     CDS             <1..512
                     /locus_tag="PW101_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2515369.1"
                     /translation="DNYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPN
                     GRKALDIETALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQ
                     RPLNENSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYV
                     QEAFYSSLI"
     gene            834..1004
                     /locus_tag="PW101_003032"
     CDS             834..1004
                     /locus_tag="PW101_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515370.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            1018..1620
                     /locus_tag="PW101_003033"
     CDS             1018..1620
                     /locus_tag="PW101_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2515371.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1636..2313
                     /locus_tag="PW101_003034"
                     /pseudo
     CDS             1636..2313
                     /locus_tag="PW101_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2624..2788
                     /locus_tag="PW101_003035"
                     /pseudo
     CDS             2624..2788
                     /locus_tag="PW101_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898188.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
     gene            2825..3121
                     /locus_tag="PW101_003036"
     CDS             2825..3121
                     /locus_tag="PW101_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HDL2515372.1"
                     /translation="MSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTNSASGVLRAN
                     EIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLKEKVVR"
     gene            3122..3538
                     /locus_tag="PW101_003037"
     CDS             3122..3538
                     /locus_tag="PW101_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HDL2515373.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3558..5102
                     /locus_tag="PW101_003038"
     CDS             3558..5102
                     /locus_tag="PW101_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2515374.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            5245..5469
                     /locus_tag="PW101_003039"
     CDS             5245..5469
                     /locus_tag="PW101_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2515375.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5484..6353
                     /locus_tag="PW101_003040"
     CDS             5484..6353
                     /locus_tag="PW101_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL2515376.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            6334..7068
                     /locus_tag="PW101_003041"
     CDS             6334..7068
                     /locus_tag="PW101_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2515377.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            7101..8009
                     /locus_tag="PW101_003042"
     CDS             7101..8009
                     /locus_tag="PW101_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HDL2515378.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            8006..8548
                     /gene="sat4"
                     /locus_tag="PW101_003043"
     CDS             8006..8548
                     /gene="sat4"
                     /locus_tag="PW101_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HDL2515379.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8641..9435
                     /locus_tag="PW101_003044"
                     /note="aph(3')-IIIa"
     CDS             8641..9435
                     /locus_tag="PW101_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDL2515380.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            9711..10283
                     /locus_tag="PW101_003045"
     CDS             9711..10283
                     /locus_tag="PW101_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL2515381.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
BASE COUNT         3705 a         1664 c         2287 g         2912 t
ORIGIN      
        1 ctgataatta tcctgatttc aattctgagt ttggtcacct tgaaggtgat acgatcgttg
       61 gcattcatca taaaagtgcc gtcattactt tagttgaaag attatctaaa gtcattatca
      121 cgattaaacc caacggccgt aaggcattag atattgaaac tgcccttaat caatggtttt
      181 ctcgcttccc taaaaacttc tttaaatcta ttacgtttga ctgtggaaaa gaattttcta
      241 actggaaagc cattagtaac caacatgata ttgatatata ttttgcggac cctggaacac
      301 cttctcaacg cccattaaac gagaattcta acgggattct gcgtcgtaat ggactgccga
      361 aatcaatgga ttttagaaaa gtgaatcaga catttatttc cagtgtcagc aatcaacgta
      421 atcatattcc aagaaaatca ttgaattaca gaacaccaat cgaaatattt ttgagctatg
      481 tacaagaagc attttattct agcttaattt gacaaatcat actatgatac gtgtttgttt
      541 ttctgtgctg tttagtgaat gattagcaga aatatagaga gtaagatttt aattaattat
      601 tagggggaga aggagagagt agcccgaaaa cttttagttg gcttggactg aacgaagtga
      661 gggaaaggct actaaaacgt cgaggggcag tgagagcgaa gcgaacactt gatcttttaa
      721 gttgctatct ttttaaggtc aatagagtat acttatttgt cctaatatgg ttttagcagt
      781 ataattgact tggtgaatag gtcatttaag ttgggcataa taggaggagt aaaatgaaaa
      841 aatttattta tcgagtttta gaaaatgacg aagtggtggc tatttttaat gagcaacaat
      901 atgcgcaaga ttttatcgct tacgaaaaga caatttctga taagcaattt gaaattgaaa
      961 aagtagatat agctgattgg ttattgcaac cgagagaatt ttagaggttg gttgaaaatg
     1021 gctaaaattg gttatgcacg tgtcagtagc aaagaacaga acttagatag acaactgaaa
     1081 gcgttagagg gcgtttctaa ggtcttttca gacaaattaa gcggtcaatc ggtcgaacgc
     1141 ccacaattac aagctatgct taactatatt cgtgaagggg atatagttgt tgttactgaa
     1201 ttagatcgat taggacgaaa taataaagaa ttaacagaat tgatgaatca aattcaaatt
     1261 aagggggcaa ccctggaagt cttaaattta ccctcaatga atggtattga agatgaaaat
     1321 ttaagacggc tgattaataa tttagtgatt gaattgtata agtaccaagc ggaatctgaa
     1381 cgcaaacgaa ttaaagaacg ccaagcccaa ggaattgaaa ttgctaagaa aaaaggaaaa
     1441 ttcaaagggc gacaacctaa attcaaagaa aatgatccac gtttacaaca cgctttcgat
     1501 ttatttttga atggttgttc cgataaagaa gttgaagaac aaactggaat taatcgccga
     1561 acgtttagaa ggtatcgatc aagatacaac gtgacagtag atcaaagaaa aaaacaatga
     1621 aaagagggat agttaatgag tacggttatt ttagctgaaa aaccaagcca ggcattagcc
     1681 tacgcaagtg ctttaaaaca aagcaccaaa aaagacggtt attttgagat caaagaccca
     1741 ctatttacag atgaaacgtt tatcaccttt ggttttgggc atttagtcga attagcagaa
     1801 ccaggtcatt ataacgaaaa gtggcagaac tggaaacttg aatctttgcc gatttttcct
     1861 gatcgatacg attttgaagt tgcaaaagat aagggaaagc agtttaaaat tgttgctgaa
     1921 cttctcaaaa aggcaaatac aattattgtc gcaacagata gcgacagaga aggtgaaaat
     1981 atcgcctggt cgattatcca taaagcaaat gccttttcta aagataaaac gtataaaaga
     2041 ttatggatca atagcttaga aaaagatgtg atccgaagcg gttttcaaaa tttgcaacca
     2101 ggaatgaatt actatccctt ttatcaagaa gcgcaaacac gccaaattgc cgattggttg
     2161 atcggcatga acgcaagccc tttatatacc ttgaatttac aacaaaaagg cgtacaaggt
     2221 acattttcat taggacgtgt acaaacaccg actttatatc tcatttatca acgccaggaa
     2281 gcaatagaaa actttaaaaa agaacctttt ttcctgaata attcatagtt ttactaatag
     2341 tctttcctaa cattgattta atagtcttta aagtattctg cttcatcacc cattaggtga
     2401 tgaaaaaaga acccctttct gatttagcgg acaaatactt tgacctaatc tcagaagata
     2461 taaaagaaaa aataataaaa tatccgattt atactttgta taaagaacta aagtagttag
     2521 caaatcattt acagcaaatc actatgttcc acataccatt ttacatactt accttatcca
     2581 cgacacatgt tgagatagta tcgaagataa agaaagttgc tccatgttgt ttttgcgaga
     2641 tggggaaatc aaagccacat tgacaaccat gatgaataag cttgctttca gtcataagct
     2701 aatcctagaa ccacttttca agtcagttag ccaaatcgat gaagaaagcg accgtgaaag
     2761 aatggacgct attgataagc taatggagca actattggaa gaacgcaata cccttattgc
     2821 actgatgtcc aaaggtttcc tagaacctgc tctttttaat caggaacgaa atgttttaga
     2881 tagtgagatt aaaaatctta ctaccgaaaa aacaaacctg gtaacgaact ctgcgagtgg
     2941 ggttttgcga gcaaacgaga taaaggacct cattaattat gtgtcagcag ataattttaa
     3001 tggtgactac acggaagaac tatttgaaga atttgtagtg aacatcattg taaattccag
     3061 ggatgagctg acattcaatt tgaaatgcgg tctttccctg aaagaaaagg tggtgagata
     3121 aatggcatat attccatatg gatacaaaat tcaagatgga gtggttactg tcgatgaaaa
     3181 ggcagcaggt caagtaaagg tattctttga gaaatacata tcaggactat cccttacagt
     3241 ggctggcgaa caggcaggta ttgaaaagac acattctgtg atggggcgca ttttgaaaaa
     3301 cgtcaactac cttggaaatg atacgtatcc agcaattatt gataaagaga tatttgataa
     3361 agctgaagaa gttagagata aacgtgcaaa ggatttagga cgagtggtag agcttgccgc
     3421 tttcacctct ccccctccca aagaacgatt caaaatgaaa aaggcagata ataagatgcc
     3481 agtggatcct tttgaacgag cagaatactt atacagtctg atagaaagcg aggaataaag
     3541 tgacagagaa aaatataatg gttattcctg ctcgtaaaag agtaggaagt acagccgcaa
     3601 aagaaaagat aaagaaactt cgtgttgctg cctattgccg tgtttctaca gaaacagaag
     3661 aacaaaattc tagctatgag gttcaggtct cacactatac agagtttata aagaaaaata
     3721 atgaatggga gtttgctggc atctttgcag atgatggtat ctctggtacg aacactaaaa
     3781 agcgtgacga atttaatcgc atgattgcag agtgtatgga cggtaacatc gacatggtta
     3841 ttacaaaatc catcagccga tttgcgcgta acaccctaga ctgccttcaa tacattagac
     3901 agctcaagga taagaacata tcagtttatt ttgagaaaga gaacatcaac accacggatg
     3961 ccaaaggtga ggttttgctg actattatgg catctttggc acagcaggaa agccagagcc
     4021 tttcacaaaa cgttaagctt gggctacagt accgatacca acaaggaaag gtgcaggtca
     4081 accataagcg atttatgggc tacacgaaag atgaagatgg aaatttaact attgtccccg
     4141 aagaagctga gattatcaaa cgcatctacc gagaatacct tgagggtcag agtctagtgg
     4201 gcattggtcg agcccttgaa aaggatggta ttttaacagc agcgggaaaa ccaagatggc
     4261 gaccagaatc agttaagaag atacttcaaa acgaaaaata catcggagat gcccttctgc
     4321 aaaagactgt cacagtagat tttctgacca agaaacgagt gaaaaatgaa gggcatgttc
     4381 cccaatatta tgttgaaaat agccatgaag cgattattcc taaagagtta tttttgcagg
     4441 cgcaagaaga aattcatcgg agaagcaata tctacacagg agaaggcaag aacaaaagaa
     4501 tttacagtag caagtacgct ttaagtgcca tcaccttctg tggagattgt ggcgatattt
     4561 atagacgggt ctattggaat atccacggaa gaaaagaatt tgtctggcga tgcgtgacta
     4621 gaatcgagca aggtcctgaa gtttgtaaga accgaaccgt aaaagaagat gaactttatg
     4681 gtgcagtaat gactgcgatt aataagctac ttgcaggtgg gaacaatatg ataaagacac
     4741 tggaagagaa tattcatgct gtgattggcg aaaccacaga ataccaaatt tcagagatta
     4801 acaactcact agaggaaaaa caaaaagaac taatcaagct ggcgaacaag ggtcaagact
     4861 atgaccactt agcagatgag attgatgagc tgagagacaa gcgacagatc cttttagtag
     4921 aagatgcctc cctcagtggc gagaacgagc gaatcaatga gctgattgaa tttatccgca
     4981 agaacaaatt ccgcacctta gaatatgatg ataagcttgt aaggaagata atccagagcg
     5041 ttacagtcta tgaaggccac ttcgtcatag ccttcaaatc tggcatcgaa atggaaatat
     5101 gaaaaccaga agtaaatagc ccatgactct acagtagagt tgtgggtttt cttttgcttg
     5161 ctgattataa taatatttta ataactattg tgtttatcaa ccaaatggtt tataatattc
     5221 ttagcgtaaa cttggaggtg ctgtatgact acggcagaaa tgattaaaga actgtgtgag
     5281 caaatgaata taagtgtttc cgaacttgct agacgtattg gccagactcc acagaatttc
     5341 aataaaaaat tacaacgaga aacagtaacc ttggatgagt tgaaggccat tgctgatgtg
     5401 ctaggtgtca agtttgtgca ggcatttatt ttaccagatg gagaagaaat aaaaatatct
     5461 aacgaataaa ggaggatgtt tatatggtag gtaatgttat tcaaatagta acagagaaat
     5521 tatcatcttt gccttttata gaaggcatcg tattaggggg ctcacgtgca agaggcaccc
     5581 atacagaaaa ttctgatata gatatcggaa tctattacaa ttcagactca tttgacctga
     5641 cagcgattaa tcaaattgct acagagttgg atgatgagaa tagaaacaac cttgttgtac
     5701 ctcccggagc gtggggtgat tgggttaatg gcggtggatg gttagttata aacgggtgtc
     5761 atgttgactt gatattacgt gatataaaac gggtggaaca aataatcaaa gatacggagc
     5821 aaggaattgt tactgccaat tatcagaccg ggcatcccca tggttatata agtgctatgt
     5881 atcgtggaga attagcgatt agtaaaatac tatatgctaa gaacgaaagc ttatgcgaat
     5941 taaaaaagca ggcagaaatt taccctactg ctctaaagaa aagcttgatg aactttttta
     6001 tatttgaagc tgaattctct ttaatgtttg taaaagcaaa tgcgggggca gaggataaat
     6061 attatattgc aggccatgtt tttcgtatta tttcatgctt aaatcaagta ctatttgcat
     6121 gtaataatgc ttattgcatt aacgaaaaga aagctataaa actgcttgaa acttttgaat
     6181 ataaacctga aaaatatgcc gaaagagtta atcatatttt tgaagtactc ggtctttctc
     6241 tttttgaatg ctacgacatg accgagaaac tttataaaga agtgaatgaa attgtatcgg
     6301 agataaataa ctttttaaac gaggagagtt cagatgaaag aaaacaaata tgatgataat
     6361 atattttttc aaaaatacag tcaaatgagt cgctcgcaga aaggactggc tggtgcggga
     6421 gaatgggaga ctttgaaaaa gatgctacct gattttaagg gtaagcgtgt gcttgattta
     6481 ggatgcggct atggatggca ctgtatatat gcgatggaaa acggtgcttc ctctgtagta
     6541 ggtgttgata tttctcataa aatgctcgaa gtagcaaaag gaaaaaccca ttttccacag
     6601 attgaatatg aatgctgtgc catagaagat gtggatttcc cagaggagag ctttgatgta
     6661 atactaagtt cgcttgcgtt tcattatgta gcagactatg agaatttaat aaaaaagata
     6721 tataggatgc tgaaggctgg tggcaattta gtttttacag ttgaacatcc tgtttttact
     6781 gctcatggaa cacaagactg gtattataac gaaaaaggag aaatactgca tttcccggtg
     6841 gacaattatt attatgaggg caaacggaca gctatgtttt tggaagaaaa ggttacaaaa
     6901 tatcatagaa cactgaccac atatctaaat acactgcttt caaatagttt tataataaat
     6961 cagattgtgg agccacagcc gccagagaac atgatggata ttccggggat ggcggatgaa
     7021 atgcgacgcc caatgatgct gattgtatcg gcaaaaaaga agatgtaata atatagaaaa
     7081 aataaacgag gagtatgtaa atgagatcag aaaaagaaat gatggattta gtactttctt
     7141 tagcagaaca ggatgaacgt attcgaattg tgacccttga ggggtcacgc gcaaatatta
     7201 atatacctaa agatgaattt caggattatg atattacata ttttgtaagt gatatagaac
     7261 cgtttatatc taatgatgac tggcttaatc aatttgggaa tataataatg atgcaaaagc
     7321 cggaggatat ggaattattc ccacctgaag aaaagggatt ttcctatctt atgctatttg
     7381 atgattacaa taaaattgat cttaccttat tgcccttgga agagttagat aattacctaa
     7441 agggcgataa attaataaag gttctaattg ataaagattg tagaattaaa agggacatag
     7501 ttccgactga tatagattat catgtaagaa agccaagcgc aagggagtat gatgattgct
     7561 gcaatgaatt ttggaatgta acaccttatg ttattaaagg attgtgccgt aaggaaattt
     7621 tatttgctat tgatcatttt aatcagattg ttcgccatga gctgctgaga atgatatcat
     7681 ggaaggtcgg catcgaaaca ggctttaaat taagtgtagg caagaactat aagtttattg
     7741 aaaggtatat atccgaggat ttgtgggaga aacttttgtc cacctaccgg atggattcct
     7801 atgaaaacat atgggaagca ttatttctat gccatcaatt gttcagggcg gtatccggtg
     7861 aggtggcgga aaggcttcat tatgcctatc cggagtatga taggaatata acaaaatata
     7921 ccagggacat gtataaaaaa tacactggta aaaccggctg cctggatagc acatatgccg
     7981 ctgatataga agagaggcgg gaacagtgat tacagaaatg aaagcagggc acctgaaaga
     8041 tatcgataaa cccagcgaac catttgaggt gataggtaag attataccga ggtatgaaaa
     8101 cgagaattgg acctttacag aattactcta tgaagcgcca tatttaaaaa gctaccaaga
     8161 cgaagaggat gaagaggatg aggaggcaga ttgccttgaa tatattgaca atactgataa
     8221 gataatatat ctttactacc aagacgataa atgcgtcgga aaagttaaac tgcgaaaaaa
     8281 ttggaaccgg tacgcttata tagaagatat cgccgtatgt aaggatttca gggggcaagg
     8341 cataggcagc gcgcttatca atatatctat agaatgggca aagcataaaa acttgcatgg
     8401 actaatgctt gaaacccagg acaataacct tatagcttgt aaattctatc ataattgtgg
     8461 tttcaaaatc ggctccgtcg atactatgtt atacgccaac tttgaaaaca actttgaaaa
     8521 agctgttttc tggtatttaa ggttttagaa tgcaaggaac agtgaattgg agttcgtctt
     8581 gttataatta gcttcttggg gtatctttaa atactgtaga aaagaggaag gaaataataa
     8641 atggctaaaa tgagaatatc accggaattg aaaaaactga tcgaaaaata ccgctgcgta
     8701 aaagatacgg aaggaatgtc tcctgctaag gtatataagc tggtgggaga aaatgaaaac
     8761 ctatatttaa aaatgacgga cagccggtat aaagggacca cctatgatgt ggaacgggaa
     8821 aaggacatga tgctatggct ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa
     8881 cggcatgatg gctggagcaa tctgctcatg agtgaggccg atggcgtcct ttgctcggaa
     8941 gagtatgaag atgaacaaag ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg
     9001 ctctttcact ccatcgacat atcggattgt ccctatacga atagcttaga cagccgctta
     9061 gccgaattgg attacttact gaataacgat ctggccgatg tggattgcga aaactgggaa
     9121 gaagacactc catttaaaga tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc
     9181 gaagaggaac ttgtcttttc ccacggcgac ctgggagaca gcaacatctt tgtgaaagat
     9241 ggcaaagtaa gtggctttat tgatcttggg agaagcggca gggcggacaa gtggtatgac
     9301 attgccttct gcgtccggtc gatcagggag gatatcgggg aagaacagta tgtcgagcta
     9361 ttttttgact tactggggat caagcctgat tgggagaaaa taaaatatta tattttactg
     9421 gatgaattgt tttagtacct agatttagat gtctaaaaag ctttaactac aagcttttta
     9481 gacatctaat cttttctgaa gtacatccgc aactgtccat actctgatgt tttatatctt
     9541 ttctaaaagt tcgctagata gagttctata ttagagatgt aaagagtttt ggtgaagagg
     9601 tttgtgagta tggcttttat aaccagggtg caatgagaag cactaacagc agctagctta
     9661 agaacgcata ggatatactt tttatggaag tcacagagga ggaataaaat ttgaacagga
     9721 ttaacagagt aacctctatt cttattcagc tgcaatcaaa aaaaataatt cctgccaaag
     9781 aaattgcaca gcgatttaat ataagcttaa ggacagttta cagagatatc cggacacttg
     9841 aagaagccgg aataccaatt ggatctgagg ccggaaaagg atattttctt gtagagggct
     9901 tcctacttcc gccggtaatg tttacagcgg cggaagtggg tgcattgatt acagcaggga
     9961 aatttttaaa ttgccatgga gatgaatcat ttataaagga ttttgattca gctatgtata
    10021 aaatcaaatc tattttgaag catggtgaaa aaaactatgc acaggagttg gagaacagta
    10081 ttaacgtgta cagcacatct ggacagaaaa atacattggc ggataacgtt attgcagcaa
    10141 ttcagactgc aatctgcaat aaaagggtaa tatcaattca atatcctgca tcaggagggc
    10201 aagaaccgga aagcagaatg atagagccta tatcactggg gttttatgaa cagaactggt
    10261 acttaatcgg ttttgcaggc taagaaatga atataggaat tttcgtgtgg atcggattaa
    10321 aagcatttgc atagaagaag agctctgtca aagccatgat ggctctttgg aacaaatatt
    10381 aaaacaaatg ctttcatata aaaaactata tgccctacaa tgccgttgta aaaattgggg
    10441 aaaagggata tattgatttt atatctttct caaaataaat ataaaagccc tttaaagagg
    10501 gcttttatat attaatcaca aatcacttat cacaaatcac aagtgattaa tcacaaatca
    10561 cttgtgat
//
LOCUS       DANKHL010000409         7958 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.guided.139, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000409 DANKHL010000000
VERSION     DANKHL010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.guided.139"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            128..1564
                     /locus_tag="PW101_003046"
     CDS             128..1564
                     /locus_tag="PW101_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2515382.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="PW101_003047"
     CDS             1753..4200
                     /locus_tag="PW101_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDL2515383.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="PW101_003048"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="PW101_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDL2515384.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="PW101_003049"
     CDS             complement(5690..6793)
                     /locus_tag="PW101_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2515385.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7345..7893
                     /gene="aac(6')"
                     /locus_tag="PW101_003050"
     CDS             7345..7893
                     /gene="aac(6')"
                     /locus_tag="PW101_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL2515386.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2665 a         1359 c         1729 g         2205 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taatttttta ttttataagt aagataaata atgaaagaat agagatactg
     7261 attggtagct aaagattttt gagcgagaac atttcgtttt ttcaaaagga ctgttagaat
     7321 aagcaagaac aagggggaag acgtatgata atcagtgaat ttgaccgtaa taatccagta
     7381 ttgaaagatc agctttctga tttactgaga ctgacttggc cggaagaata tggagacagc
     7441 tcggcagaag aagtagaaga aatgatgaat ccagaacgaa tcgcggtagc agcggtagac
     7501 caagatgagt tagtaggatt tattggtgca atccctcaat acggtatcac aggttgggaa
     7561 ttgcatccat tagttgtaga aagctcccga cgaaagaacc aaataggtac tcgattagtc
     7621 aattacttag aaaaagaagt agcttccaga ggaggaatca cgatttattt aggtacggat
     7681 gatttagacc atggaacaac gttaagtcaa acggacctgt atgagcatac atttgataaa
     7741 gtggcttcca tccagaacct tcgtgaacat ccgtatgaat tctatgaaaa attaggttat
     7801 aaaatcgtag gtgtcttacc aaatgcaaat ggctgggaca aaccggatat ttggatggca
     7861 aaaacgatta ttcctcgacc aaattctcaa taataaaaag aggttgtgac agaagtaatc
     7921 agcttcaaga aataagaagg aatttacgaa aattactt
//
LOCUS       DANKHL010000410         7474 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.guided.147, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000410 DANKHL010000000
VERSION     DANKHL010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.guided.147"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..398
                     /locus_tag="PW101_003051"
     CDS             <1..398
                     /locus_tag="PW101_003051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515387.1"
                     /translation="LNGLEKLREIFKTAVFNSNQSVLLTVSPQLLNNPRFLAMQIEQM
                     YEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMVLSNVWLNPLVEMTDKEGMEKRC
                     NTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            505..1743
                     /locus_tag="PW101_003052"
     CDS             505..1743
                     /locus_tag="PW101_003052"
                     /inference="COORDINATES: protein motif:HMM:NF017760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2515388.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            1970..2389
                     /locus_tag="PW101_003053"
     CDS             1970..2389
                     /locus_tag="PW101_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2515389.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            2394..2633
                     /locus_tag="PW101_003054"
     CDS             2394..2633
                     /locus_tag="PW101_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2515390.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(2858..3781)
                     /locus_tag="PW101_003055"
     CDS             complement(2858..3781)
                     /locus_tag="PW101_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDL2515391.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(3813..4577)
                     /gene="map"
                     /locus_tag="PW101_003056"
     CDS             complement(3813..4577)
                     /gene="map"
                     /locus_tag="PW101_003056"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL2515392.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4735..5175
                     /locus_tag="PW101_003057"
     CDS             4735..5175
                     /locus_tag="PW101_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDL2515393.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            complement(5353..5922)
                     /locus_tag="PW101_003058"
     CDS             complement(5353..5922)
                     /locus_tag="PW101_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2515394.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            6177..7409
                     /locus_tag="PW101_003059"
     CDS             6177..7409
                     /locus_tag="PW101_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDL2515395.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT         2377 a         1306 c         1387 g         2404 t
ORIGIN      
        1 gtttaaacgg tttagaaaag ttaagagaaa tatttaagac agcggtattc aattctaacc
       61 aaagcgtttt attgacagtt tctcctcaac tattaaacaa tcctcgtttt cttgctatgc
      121 aaatagaaca aatgtatgaa attgtagtac ctgaatttgt tgaacccgtt ttagaacaag
      181 gtatcaagga taagtctttg gagattgaaa attcacatga aattgcagaa gttatcatgg
      241 tattaagtaa tgtatggctc aacccattag ttgaaatgac ggataaagag ggtatggaaa
      301 aacgttgcaa tacattcaat gcccttttaa atggaatagg catagacaca ttattagacg
      361 aagaaacaat ttctgcattt atacagtttt gtaaataact gtatttgtta tccctatacg
      421 atagactgct ctataaaaga gcagtttatt taaaccatta tataaaccga cggtctgttt
      481 gattaaataa aaaggagaga ttttatgcaa gcagaaaaaa aactttttaa tcgtgatttt
      541 atacttgttg ttataggaca gataatttct ttatttggaa acgctatcct acgatttgca
      601 ttaccgtact atttgttaaa agaaactggt tcatcagctt tgtttggtat tgctacagcc
      661 tgctcttttt tacctatggt tattttgtcg ctggtaggtg gtattattgc tgacaggaca
      721 aataaaagaa acattatggt catactagat tttgtaacag caatcataat tttagtattt
      781 tatttgtcat tgggaaaaat tcctatcata cctttgttta ttgttgcttt gatgttactt
      841 tatggtattt ctggtgcata tcaaccagcg gtacaagcaa gtattcctct attggtatca
      901 tcagataaac ttaccgtcgg taatgctata ataaatcaag taaatacttt agcaaacttt
      961 atcggaccag ctataagcgg agtagttttt caattttatg gtatcaatcc cattttaatt
     1021 gttagtatta tatgttttac tttttcagcc attatggaaa tttttatcca tattccacac
     1081 caaaaaagac ctcgtgaagt aggtgtaatt gcagttgcta aaaacgattt aaaagatagt
     1141 tatacctttt tgaaaaagga gaaacccatt tttatatccg taatcttttt ggtttgcatt
     1201 ttcaatttgg ttttaagtgc tgttttaatt gttggtctgc ctatcttaat ttctgaagtt
     1261 ttaaaaatgc cagatacact atatggtttt tcacaatcag cattatcatt aggtggttta
     1321 tgtggtggta ttttaacagc tcttgttgct tcaaagttaa aactaaaaaa cctttatacc
     1381 ttgttactta tttgttctgt ttctgtttta cttatgggaa tttcgctatt tttagaattg
     1441 cctgcaatag tttcctactg ggtagttaca gtgatgtgct ttgtggctat gggtgtatct
     1501 actatgtttg ttgttcaaat ttacactctt gttcagacac aaacaccacc gcaattagta
     1561 ggaaagataa tggctacgct ggtagcagtt gctatgtgcg gtcaacctat tggacaaatg
     1621 gtttacggta ttttattcga tgtatttata caagaaacat gggttgttat gtttgtagct
     1681 tctattgctt caatcatgat ttcaatatat tcaaaaagaa tttttcaaaa gttagagata
     1741 taatacaagg ggttaaatag tatgttaggc taaaaacaat atataaaaga aaatttgaaa
     1801 tctgctaaaa ggatagtgtt ataaaagaaa gagtaatatt gcagtattta aggctcgcac
     1861 aaatatatgt tttgtgtggg cttttttctt accatttttc tgtatttcta aagggtataa
     1921 acggtattcc gtttatatat ttcagcatta gcagaaaggg ggtgagatta tgaggacatc
     1981 ttctttcgag catatcgtca gattacagtt caattcttta atgctgattg tcattaagaa
     2041 caaagtgaaa agtcgaaaca gacagcttgc aagacattct aaacgtgaag tattgttttg
     2101 tgaaatagcc gaaacgaaac aagttgaacg tggaacgatt gatacctgtt tttatgattg
     2161 tgtatcattt aaagtcttgc actttacaat ttatgtatct gatgaaacac ttggtacagt
     2221 tttacataga ctgtcagaaa aacagcgtag tgcgatttta ttacgttatt ttcaaggaat
     2281 gaacgacaga gaaatttcag aactgtacca tgtatcacgt tctgctattt gcagtagaag
     2341 aactagagga ctaaggaagt taaaaatgct tttgagcgaa aggaagtaaa gaaatgagta
     2401 aagattatgg ctatccctct tttgaattga tttgcaaagc gtcaagcgga gatgaaatgg
     2461 cagttaaaga gattttgaag ttttatgacg gttatatttc taaattatgc ttgcgtcctt
     2521 tttaccactc tgaaagtggt aaaatcatta tgcaggttga tgaagaacta aaaggagaaa
     2581 ttcatacaga catgatgaaa gcaatattga aatttgaaat cggagtgaag taatcaactg
     2641 tataaaaaaa tcgggagata cacagtttcg tgctatctcc cttagtagtt ttacggtcat
     2701 atcaaggctc attcaatcgt ggtaaattcg tggtaagatg agtgtttttc tatacttcaa
     2761 aatgcttgaa tgtatagtgt ttatgcggat aattgaaaat tagatataaa atttcaaata
     2821 aattactgat ataaatttac aataagaact cttgtttcta ctttttttta tgagaaaatt
     2881 tagttttcaa tttattgaac cattttcggc ttggatctcg acgttttttg atttgatgac
     2941 ccgtcacgta ttcttgaaca actgcattga tgattccgcc caaaatgatg atcgtagcag
     3001 caaaattaag ccaaagcatg agtacaataa aacttcctat gatctgataa ccgctgatcc
     3061 tcgttgcaaa atacctagca tagatactaa acacttgtga taacagcatc cagccgacag
     3121 tagcgaagat cgtgcccgga atgatcgtct tcaacttcaa ctgagcatta gggacaatta
     3181 aataaatcaa gaacatgatg acaaataaag ctaccaatgt gattggccac ttcaaagttt
     3241 ggaactgatc gataaagttc acgggtattt gtacgattgg ctgaatagca tccaaaatca
     3301 gctgtcccaa cccaagaacg atagttaccc cactaatagc aatcatgaac aagacaatga
     3361 caattagtga gaagaaacgg acgatcaaaa aattttttct attttctacg ccatacgctt
     3421 tattcatcgc aatttgcaaa gcattgatac tttgacttgc agaccataaa gccgctaatg
     3481 cagaaacgga taacaagcct cctgaacttt gtgtcaaaag gtctttgatt gcaggaccga
     3541 taaactggta aacggtctca ggaatcactt ctcgtaaata ggtcagtaca gtctcttgat
     3601 cgatgtggag aaacggtaaa atattaccaa aagcaataat caatggaaat aaagacaaga
     3661 gtaaataata agcaacgacg acagaggtcg tactcatttc tgaatcagtg atgcgctttt
     3721 gagtcgtctc aataaagcgc atcagatttt cattttgttt tattttgttc caaactttca
     3781 tgtacctctc ccgtacctat tgactggata gatcaataag tgccttcttc tccttgtgat
     3841 gtcaaaattc ttggaccatc tttagtaata gccaatgtat gttcatattg gcaagaaagt
     3901 ccgccgtctc ttgtatacgc agtccagccg ttaggatcca ttttcatttt ccaagttccg
     3961 gtattaacca taggttcgat cgtgataacc atgccttctt tcaaccgcaa cccttttcca
     4021 gcttctccgt aatgaggaac tgctggttct tcatgaatcg taggaccaac gccatgacca
     4081 ataaaatcac gaacgatcgc tagattttcg ccttctacat aagtttggat agcatgaccg
     4141 atgtcaccaa tccgattccc tacttgtgct tgctcaatac cgatgtacaa agctttcttc
     4201 gttacttcca tcaatcggtc gatctcaggt gtagattctc caactacata ggcccaacaa
     4261 gaatcagaaa tgccgccttt caaatcgata cacatatcga cttttaccaa atcaccgttt
     4321 ttcaaaggct ttttacgagg gaatccatga caaatttcat catttatact gatacaagtc
     4381 gcatattcat acccttcaaa gccaatttgc gcagcaattc cgccatgact ctcgatatag
     4441 tttctgacaa atacctcaat atcccaactt gtgattcctg gttttataaa atcacgcaga
     4501 tttttatgta catctgctaa taaagctcca gattctgcca tcatttcaat ttcacgtgct
     4561 gattttaaag taatcatatt ttcgtctcct ttttacaaat ccctctcatt ttaacatagc
     4621 ggcatacatt tagccaaatg acattcccca ggtgtctcta ggttttattt tcttttcaat
     4681 gatgttttgg tataattcat aattgttaaa taattataaa cgaggaggca catcatggct
     4741 ttagcaaaaa tcgtatatgc tagcatgaca ggaaacactg aagaaatcgc cgatattgtt
     4801 gcagaagcat ttgaaaatct ggatatcgaa gtcgaaatca acgaatgtac ccaagtagat
     4861 gcagatgaat tcgaggaggc agatatttgc gtagtagcta cttatacata cgatgatggc
     4921 gatctgcctg atgagatcgt agacttttat gaagacttgc aagaattgga tctattggga
     4981 aaaatttacg gtgtctgcgg atctggtgat actttttacg atgaattctg caaatcggtg
     5041 gatgatttcg atgctgcttt tgcaaaaaca ggcgcatcaa aaggagccga aaatgttaaa
     5101 gtggatctga atgctgaaga agaagatatc gaaaatttag aagctttcgc taaagaacta
     5161 gtcgcaaaaa tttaatatac ttgcaataaa agagccatcc agatttattc aatctgtatg
     5221 gctcttttat tcattgcatt catggaagag ttcttcggga gttaaaaatg catacgctgt
     5281 aagaaaaacg aacttttcct tattttgaca catctcttgt tacctactga tttgtggaac
     5341 ttttgagtat attcaatcga tttcgattgg ttctttgaat ttcaagatag catctatact
     5401 gtcttggatc atcttttcaa tctgttcatc agttggatgg atgatgcttg aagacatcaa
     5461 agctgcgatc ccagtagcta tgatccatgt cccattatac aaagattcga tctgctcttc
     5521 tgaaagattc acataagctg gatcgcgttt cactgtattc acaaagtaag tataagagaa
     5581 attctgcatc tcttttcctc caccatattc ttctaagtac agtgaacgat acaacttctt
     5641 ctctcttctt gcaaaatgga tataattaaa agcaagatcg ataatcgtat tccctgtatg
     5701 ctcaacagtg aacacttcat attgtaaatg atcatagatc tgctcaaaaa gagcttgtct
     5761 caagtcatcc atatttttaa actcaagata aatcggttgc gtggaacaat tcattttgtt
     5821 cgcgatgttt ctcgcagtaa atcgtgtaaa tccatcggtg gcagctactt cataagcagc
     5881 tgccaaaatc tgttctctag taatcgtctt ttttcttgcc atatttttta atctccttaa
     5941 tttttttata acgcttccac cattgattaa tttcattgta acatgttttt tttgatatgc
     6001 aaaattagtt tttgattttc ttcacaagct gaaatagcaa gtagtaacat atatcatgaa
     6061 acttaacctt aaaaaaataa cctccttttt ttatttaaag cctcattgtt tgaatcataa
     6121 ttaaatggta tcatgctaat agttaccaga aaaaatggtg aggaggaatt tttattatgt
     6181 tatctgattt taatgaactt ctaaaaaaat atgcacgctt aattgcagaa actggcgtag
     6241 ccacagaaaa aggacacaca gttgttttac agatcagcgt ggaacaggca ccccttgctc
     6301 gattgatcac acaagaagct tataaactag gagccgctga ggttatcgtt caatggactg
     6361 acgaccagat ccaaagagaa tttcttttac atgctgcaac ggatcgtatt gagaatgtac
     6421 cacaatctaa aatcgatcaa gcggatgaat ggcttgaaaa aggagcaagc aggatcagcg
     6481 ttgtttctgc cgatcccgat gcatttgctg gtgtagatag ccatcgagta gcgtcttatc
     6541 aatcagcgaa tggcaaagca ctaatgaatt tacgcaaggc aactcaagca aataaagtca
     6601 gttggactgt cgtggctgcg gctggtaaac aatgggcagc aaaagtcttc ccagaactgc
     6661 cagaagaaaa acaagttgag gcattatggg atcagatttt caaaacgact cgtgtctatg
     6721 aagaagaccc tgtcttagca tggaagaagc atgatgaaaa attagcaaaa aaagcagaag
     6781 aattgaatcg agaacaattt tctgcccttc actatactgc tcccggtaca gatatcatta
     6841 tcggattgcc taaaaaccat ctttgggaag gcgctggaag ctataatgct cgtggagaaa
     6901 aattcatggc aaatatgccg acggaagaag tgttcacagc acctgacagt catcgagtag
     6961 atggttacat ttcaagtaca aagccactga gttatgctgg caccatcatc tcgggtatga
     7021 aatttacttt taaagatgga aaagttgttg atttttctgc cgaacaagga gaagatgtct
     7081 tagcaaaact gcttgataca gatgaaggtg cgcgtcgttt aggagaagtc gcacttgtac
     7141 ctgacccttc acctatttct caatctggca tcatcttttt caatacatta tttgatgaaa
     7201 acgcatcgaa tcacttagca ctaggttctg cttatgcctt cagtgtgaaa ggcggtacag
     7261 aaatgtccga tgaagaatta gcagaagctg gattgaatag aagtcaaacc cacgtagatt
     7321 tcatggtagg atctgacaag atggatatcg acggaatccg tgaagatggc tctacagtac
     7381 cgattttccg aaatggcgat tgggcttgag tatactttcg cacacatgca aaaaataatg
     7441 gctctttgtc aataaggact gatgagttgt gcaa
//
LOCUS       DANKHL010000411         6558 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.guided.161, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000411 DANKHL010000000
VERSION     DANKHL010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.guided.161"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..413
                     /locus_tag="PW101_003060"
     CDS             <1..413
                     /locus_tag="PW101_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2515396.1"
                     /translation="DDENQIRAFDAEADDYVTKPFKMQILLKRVEALLRRSGALAKEI
                     RVGRLTLLPEDFTVLCDGTELPLTRKEFEILLLLVQNKGRTLTHEIILSRIWGYDFEG
                     DGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEES"
     gene            416..1759
                     /gene="vanS"
                     /locus_tag="PW101_003061"
     CDS             416..1759
                     /gene="vanS"
                     /locus_tag="PW101_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDL2515397.1"
                     /translation="MERKGIFIKVFSYTIIALLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQILAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQAQSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            1930..2736
                     /gene="vanY"
                     /locus_tag="PW101_003062"
     CDS             1930..2736
                     /gene="vanY"
                     /locus_tag="PW101_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HDL2515398.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            2754..3581
                     /gene="vanW"
                     /locus_tag="PW101_003063"
     CDS             2754..3581
                     /gene="vanW"
                     /locus_tag="PW101_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HDL2515399.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGEPFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            3578..4549
                     /locus_tag="PW101_003064"
     CDS             3578..4549
                     /locus_tag="PW101_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HDL2515400.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            4542..5570
                     /gene="vanB"
                     /locus_tag="PW101_003065"
     CDS             4542..5570
                     /gene="vanB"
                     /locus_tag="PW101_003065"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HDL2515401.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGTFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            5576..6184
                     /locus_tag="PW101_003066"
     CDS             5576..6184
                     /locus_tag="PW101_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HDL2515402.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(6250..6423)
                     /locus_tag="PW101_003067"
     CDS             complement(6250..6423)
                     /locus_tag="PW101_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515403.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1857 a         1384 c         1721 g         1596 t
ORIGIN      
        1 cggatgacga aaaccaaatc cgggcgtttg atgcagaggc agacgactat gtaacaaagc
       61 cattcaagat gcagatttta ctaaagcggg tggaagccct gctgcggcgc agcggtgcgc
      121 tggcaaagga aatccgtgtc ggcaggctga cacttctgcc ggaggatttc acggtacttt
      181 gtgacggtac agagctgccc ctgacacgaa aagaatttga aatccttttg ctgctggtgc
      241 agaacaaagg cagaacctta acgcatgaaa tcattttgtc acgtatatgg ggatatgact
      301 ttgagggtga tggcagcaca gtccacactc atatcaaaaa tttgcgggcc aagctgccgg
      361 aaaatatcat caaaaccatt cgcggtgtag gataccgatt ggaggaatca taataatgga
      421 aagaaaaggg attttcatta aggttttttc ctatacgatc attgccctgc tactgcttgt
      481 cggtgtaaca gcaacactgt ttgcacagca atttgtgtct tatttcagag tgatggaatt
      541 acagcaaaca gtaaaatcct atcagccatt ggtggaactg attcagaata gcgataggct
      601 tgatattcaa gaggtggcag ggctgtttca ctacaataac caatcctttg agttttatat
      661 tgaagataaa gagggaagcg tactctatgc cacaccaaat gccaatacat caaatagttt
      721 tagacccgac tttctttatg tggtacatag agatgataat atttcgattg ttgctcaaag
      781 caaggcaggt gtgggattgc tttatcaagg gctgacaatt cggggaattg ttatgattgc
      841 gataatggtt gtattcagcc ttttatgcgc gtatatcttt gcgcggcaaa tgacaacgcc
      901 gatcaaagcc ttagcggaca gtgcgaataa aatggcaaac ctgaaagatg taccgccgcc
      961 gctggagcga aaggatgagc ttggcgcact ggctcatgat atgcattcca tgtatgtcag
     1021 gctgaaagaa accatcgcaa ggctggagga tgaaatcgca agggaacatg agttggagga
     1081 aacacagcga tatttctttg cggcagcttc tcatgagcta aaaacgccca tcgcggctac
     1141 aagcgttctg ttggagggaa tgcttgaaaa tatcggtgac tacaaagatc attctaagta
     1201 tctgcgcgaa tgcatcaaaa tgatggatag gcagggcaaa atcatttccg aaatactgga
     1261 gcttgtcagc ctgaatgatg ggagaatcgt acccatagct gaaccgttgg acatagggcg
     1321 cacggttgcc gagttgctgc ccgattttca aatcttggca gaggcaaaca accagcggtt
     1381 cgtcacagat attccagccg ggcaaattgt cctgtccgat ccgaggctgc tccaaaaggc
     1441 actatccaat gtcatattga atgcagttca gaacacgccg cagggaggcg aggtacggat
     1501 atggagtgag cctggtgctg aaaaatgccg cctttttgtt ttgaacatgg gcgttcacat
     1561 tgatgatact gcgcttccaa ggctgttcac cccattctat cgcattgatc aggcgcaaag
     1621 cagaaaaagt gggcgaagcg gtttaggact tgccatcgta caaaaaacgc tggatgccat
     1681 gagcctccag tatgcgctgg aaaacacctc ggatggcgtt ttgttctggc tggatttacc
     1741 gctcacatca acattgtaaa tatttaaaac ttaaatgatt ttgaccgaca ggtataaccc
     1801 tgccggtctt tttttgctgc tacaggaaaa ctacagactg gctacaggga aagtacagat
     1861 gcgcttgcca taataacaat cgtaccagcc acaaatcgta gttttattgc aaaggaggca
     1921 ttcgatcaaa tggaaaaaag caactatcat tccaatgcgg atcatcacaa acggcatatg
     1981 aaacaatccg tggaaaagcg ggcttttcta tgtgcgttca ttatttcgtt cacagtctgc
     2041 acgctgtttt tggggcggag actggcttcc gtattggagg caacacagat accgcccatc
     2101 cctgcaactc atacaggcag cagcactgac gtagtggaga atttggagga aaacgctctt
     2161 gccaccgcca aagaacaggg agatgaacag gaatggagcc tgattttagt gaacaggcag
     2221 aaccccatcc ccgcacagta cgatgtggag cttgagcaac tatcaaatgg tgagcggata
     2281 gatattcgga tttctcccta tcttcaagat ttgtttgatg ccgcaagaac tgatggagtt
     2341 tacccgattg tcgcatccgg ataccgaaca acagaaaaac agcaagaaat tatggatgaa
     2401 aaaattgccg aatataaggc gaaaggctac acctctgcac aggctaaagc ggaagcagaa
     2461 acttgggtgg ccgtgccggg aacgagcgag catcagcttg gtcttgctgt ggatatcaat
     2521 gcggacggaa ttcattcaac aggcaacgag gtttatagat ggctggatga aaacagctat
     2581 cgttttggtt ttattcgtcg ctacccacca gacaagacag agataaccgg tgtgagcaac
     2641 gagccgtggc attaccgata tgttggcatc gaagctgcca cagagatgta caaccaaggg
     2701 gtttgccttg aggaatattt aaaaccagaa aaatgagaaa aggatgttat gttatggaca
     2761 gaaaaagatt gacacagcgt ttcccgttcc tgcttccaat gagacgggcg cagagaaaaa
     2821 tgtgctttta tgcgggaatg agatttgacg gctgtcgcta tgcgcagacg ataggggaaa
     2881 aatcgctttc ccatttgctc tttgaaacgg attgcgcatt atacaaccac aataccggat
     2941 ttgacatgat ataccaggaa aacaaggtgt tcaacttaaa gctggcagca aagaccttaa
     3001 acggcttatt gataagaccg ggggaaccct tttctttctg gtggctggta cgccatgcgg
     3061 acaaagatac cccctataaa gacggcctta cggtgaccaa tggtaaactc accaccatgt
     3121 cgggcggcgg tatgtgccag atgagcaatt tactattttg gatgttcctg catacgccat
     3181 tgacaattat ccagcgcaga ggtcacgaag taaaggagtt tccagagcca aacagtgacg
     3241 agattaaagg ggtggatgca accatttcag agggctggat tgatttaaaa gtgcgaaacg
     3301 ataccgactg cacctaccaa atatgggtga ccttagatga tgagaaaatc atcggtcagg
     3361 tgtccgccga caaagagccc caagcattat acaaaattac aaatggcagt atccagtatg
     3421 tccgtgaaag tggcgggatt tatgaatatg cccaggttaa acggatgcaa gttgccttag
     3481 gtaccgggga aataatagat tgcaagctgc tttatacaaa caaatgcaaa atctgttatc
     3541 ccctcccgga aagtgtggat attcaggagg agaaccaatg agaaaaagtg tgggcattac
     3601 tgtttttgga tgcgaacagg atgaggcaaa tgctttccgc gctttatcgc cggattttca
     3661 tattatccct acgctgatta gcgatgcgat atcggcagac aacgcaaaat tggccgctgg
     3721 caatcaatgc gttagcgtag gccataagtc cgaggtttcc gaggcgacaa ttcttgcgct
     3781 gagaaaggtc ggggtaaaat acatttctac ccgcagcatc ggctgcgatc acattgatac
     3841 gactgccgcc gagagaatgg gaatctcggt tggcacggtt gcgtattcgc cggacagcgt
     3901 tgcggattat gctttgatgc tgatgctgat ggccatacgg ggtgcaaaac ccaccatgca
     3961 cgccgtggcg caacaagatt tcagattgga tcgtatccgg gggaaagaac tgggggatat
     4021 gactgtggga gttattggaa ccggccatat cgggcaagcg gtcgtcaaaa ggctgcgggg
     4081 atttggatgc catgtgctgg cctatgataa cagccgaaaa atggatgcag attatgtcca
     4141 gcttgatgag cttctaaaaa acagcgatat tgttacgctc catgtgccgc tttgtgcgga
     4201 tacccgccat ctgatcggtc agaagcaaat tggagagatg aagcaaggcg catttttaat
     4261 caacaccggg cgcggagcgc ttgtcgatac cggggcgctg gtggaggcgt tggaaagcgg
     4321 aaagctgggt ggtgcggcac tggatgtgtt ggagggcgag gatcagtttg tttataccga
     4381 ttgctcgcag aaagtgcttg accatccctt tttgtcgcag cttttaagga tgccgaatgt
     4441 gatcatcaca ccccatacgg cgtactacac tgagcgtgtg ctgcaggata ctacagaaaa
     4501 aacaatcagg aattgtctca attttgaaag gagtttacag catgaataga ataaaagtcg
     4561 caatcatctt cggcggttgc tcggaggaac atgatgtgtc ggtaaaatcc gcaatagaaa
     4621 ttgctgcgaa cattgatacg gaaaaattcg atccgcacta catcggaatt acaaaaaacg
     4681 gtgtatggaa gctatgcaag aagccatgta cggaatggga agccgacagt ctccccgcca
     4741 tactctcccc ggataggaaa acgcatgggc tgcttgtcat gaaagaaagc gaatacgaaa
     4801 cacggcgtat tgatgtggct ttcccggttt tgcatggcaa atgcggggag gatggtgcga
     4861 tacaggggct gtttgtattg tctggtatcc cctatgtggg ctgtgatatt caaagctccg
     4921 cagcttgcat ggacaaatca ctggcctaca ttcttacaaa aaatgcgggc atcgccgttc
     4981 ccgaatttca aatgattgat aaaggtgaca agccggaggc gggtgcgctt acctaccctg
     5041 tctttgtgaa gccggcacgg tcaggttcgt cctttggcgt aaccaaagta aacggtacgg
     5101 aagaacttaa cgctgcgata gaagcggcag gacaatatga tggaaaaatc ttaattgagc
     5161 aagcgatttc gggctgtgag gtcgggtgtg cggtcatggg gaacgaggat gatttgattg
     5221 tcggcgaagt ggatcaaatc cggctgagcc acggtacctt ccgcatccat caggaaaacg
     5281 agccggaaaa aggctcagaa aatgcgatga ttacagttcc cgcagacatt ccggtcgagg
     5341 aacgaaatcg ggtgcaggaa acggcaaaga aagtatatcg ggtgcttgga tgcagagggc
     5401 ttgcccgtgt tgatcttttt ttgcaggagg atggcggcat cgttctaaat gaggtcaata
     5461 ccctgcccgg ttttacatcg tacagccgct acccacgtat gatggccgcc gcaggaatca
     5521 cgcttcctgc actgattgac agcctgatta cattggcgtt aaagaggtga taagtatgga
     5581 aaatggtttt ttgtttttag atgaaatgtt gcatggcgtc cgttgggatg ccaagtacgc
     5641 cacatgggat aacttcacag gaaaaccggt ggatgggtat gaggtgaatc gcatcatcgg
     5701 cacaaaggcc gtggcgtttg ctctgcgcga ggcacaaatc catgcggctg cgcttggcta
     5761 tggcttgctt ttatgggatg gatatcggcc aagaactgcg gtggactgct tcctgcgttg
     5821 ggcagcgcaa ccggaggaca atctcacaaa agaaaaattt taccccaata tagagcgagc
     5881 cgagttgatt acaaagggtt atgtggcctc acaatccagc catagccgtg gaagcgcaat
     5941 tgatcttacg ctctaccacc tggatacagg ggaacttgtt tcaatgggaa gtaacttcga
     6001 ttttatggac gaacggtcgc atcatacagc aaaagggata ggggatgcag aggcacaaaa
     6061 tcgaagatgc ttgcgtaaaa tcatggaaag cagcggattt cagtcttatc gctttgaatg
     6121 gtggcactat aagttgattg atgagccata ccccgatacc tattttaatt ttgccgtttc
     6181 ataatgaaag tattggtttt gtatcagatt ttggatttga taaaatctat gctggtcatg
     6241 gacaatcttt tactggaaag tggttaaagc atcttttgta atacattgca aattatagta
     6301 ttcaatctgc ccagcggcag aaaagaaaaa aaccgctgtt gggcagacct ttcccacgcc
     6361 cattttgtat tttgctggcg gcggttttga gaaatcaagg ccgccgttct tttctggcag
     6421 caaaaggtgt ggcggcccat aggaggatgc cgctatgctg ttttgctatt gccatgctct
     6481 aatccacttc caccactcaa aaaaagcctt gcccctccat gaactgaacc ccaaaagttg
     6541 aactatttaa tggactgt
//
LOCUS       DANKHL010000412         6558 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.guided.162, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000412 DANKHL010000000
VERSION     DANKHL010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.guided.162"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..413
                     /locus_tag="PW101_003068"
     CDS             <1..413
                     /locus_tag="PW101_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2515404.1"
                     /translation="DDENQIRAFDAEADDYVTKPFKMQILLKRVEALLRRSGALAKEI
                     RVGRLTLLPEDFTVLCDGTELPLTRKEFEILLLLVQNKGRTLTHEIILSRIWGYDFEG
                     DGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEES"
     gene            416..1759
                     /gene="vanS"
                     /locus_tag="PW101_003069"
     CDS             416..1759
                     /gene="vanS"
                     /locus_tag="PW101_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDL2515405.1"
                     /translation="MERKGIFIKVFSYTIIALLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHGMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQAQSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            1930..2736
                     /gene="vanY"
                     /locus_tag="PW101_003070"
     CDS             1930..2736
                     /gene="vanY"
                     /locus_tag="PW101_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HDL2515406.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            2754..3581
                     /gene="vanW"
                     /locus_tag="PW101_003071"
     CDS             2754..3581
                     /gene="vanW"
                     /locus_tag="PW101_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HDL2515407.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGEPFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            3578..4549
                     /locus_tag="PW101_003072"
     CDS             3578..4549
                     /locus_tag="PW101_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HDL2515408.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            4542..5570
                     /gene="vanB"
                     /locus_tag="PW101_003073"
     CDS             4542..5570
                     /gene="vanB"
                     /locus_tag="PW101_003073"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HDL2515409.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGTFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            5576..6184
                     /locus_tag="PW101_003074"
     CDS             5576..6184
                     /locus_tag="PW101_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HDL2515410.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(6250..6423)
                     /locus_tag="PW101_003075"
     CDS             complement(6250..6423)
                     /locus_tag="PW101_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515411.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1856 a         1385 c         1722 g         1595 t
ORIGIN      
        1 cggatgacga aaaccaaatc cgggcgtttg atgcagaggc agacgactat gtaacaaagc
       61 cattcaagat gcagatttta ctaaagcggg tggaagccct gctgcggcgc agcggtgcgc
      121 tggcaaagga aatccgtgtc ggcaggctga cacttctgcc ggaggatttc acggtacttt
      181 gtgacggtac agagctgccc ctgacacgaa aagaatttga aatccttttg ctgctggtgc
      241 agaacaaagg cagaacctta acgcatgaaa tcattttgtc acgtatatgg ggatatgact
      301 ttgagggtga tggcagcaca gtccacactc atatcaaaaa tttgcgggcc aagctgccgg
      361 aaaatatcat caaaaccatt cgcggtgtag gataccgatt ggaggaatca taataatgga
      421 aagaaaaggg attttcatta aggttttttc ctatacgatc attgccctgc tactgcttgt
      481 cggtgtaaca gcaacactgt ttgcacagca atttgtgtct tatttcagag tgatggaatt
      541 acagcaaaca gtaaaatcct atcagccatt ggtggaactg attcagaata gcgataggct
      601 tgatattcaa gaggtggcag ggctgtttca ctacaataac caatcctttg agttttatat
      661 tgaagataaa gagggaagcg tactctatgc cacaccaaat gccaatacat caaatagttt
      721 tagacccgac tttctttatg tggtacatag agatgataat atttcgattg ttgctcaaag
      781 caaggcaggt gtgggattgc tttatcaagg gctgacaatt cggggaattg ttatgattgc
      841 gataatggtt gtattcagcc ttttatgcgc gtatatcttt gcgcggcaaa tgacaacgcc
      901 gatcaaagcc ttagcggaca gtgcgaataa aatggcaaac ctgaaagatg taccgccgcc
      961 gctggagcga aaggatgagc ttggcgcact ggctcatggt atgcattcca tgtatgtcag
     1021 gctgaaagaa accatcgcaa ggctggagga tgaaatcgca agggaacatg agttggagga
     1081 aacacagcga tatttctttg cggcagcttc tcatgagcta aaaacgccca tcgcggctac
     1141 aagcgttctg ttggagggaa tgcttgaaaa tatcggtgac tacaaagatc attctaagta
     1201 tctgcgcgaa tgcatcaaaa tgatggatag gcagggcaaa atcatttccg aaatactgga
     1261 gcttgtcagc ctgaatgatg ggagaatcgt acccatagct gaaccgttgg acatagggcg
     1321 cacggttgcc gagttgctgc ccgattttca aaccttggca gaggcaaaca accagcggtt
     1381 cgtcacagat attccagccg ggcaaattgt cctgtccgat ccgaggctgc tccaaaaggc
     1441 actatccaat gtcatattga atgcagttca gaacacgccg cagggaggcg aggtacggat
     1501 atggagtgag cctggtgctg aaaaatgccg cctttttgtt ttgaacatgg gcgttcacat
     1561 tgatgatact gcgcttccaa ggctgttcac cccattctat cgcattgatc aggcgcaaag
     1621 cagaaaaagt gggcgaagcg gtttaggact tgccatcgta caaaaaacgc tggatgccat
     1681 gagcctccag tatgcgctgg aaaacacctc ggatggcgtt ttgttctggc tggatttacc
     1741 gctcacatca acattgtaaa tatttaaaac ttaaatgatt ttgaccgaca ggtataaccc
     1801 tgccggtctt tttttgctgc tacaggaaaa ctacagactg gctacaggga aagtacagat
     1861 gcgcttgcca taataacaat cgtaccagcc acaaatcgta gttttattgc aaaggaggca
     1921 ttcgatcaaa tggaaaaaag caactatcat tccaatgcgg atcatcacaa acggcatatg
     1981 aaacaatccg tggaaaagcg ggcttttcta tgtgcgttca ttatttcgtt cacagtctgc
     2041 acgctgtttt tggggcggag actggcttcc gtattggagg caacacagat accgcccatc
     2101 cctgcaactc atacaggcag cagcactgac gtagtggaga atttggagga aaacgctctt
     2161 gccaccgcca aagaacaggg agatgaacag gaatggagcc tgattttagt gaacaggcag
     2221 aaccccatcc ccgcacagta cgatgtggag cttgagcaac tatcaaatgg tgagcggata
     2281 gatattcgga tttctcccta tcttcaagat ttgtttgatg ccgcaagaac tgatggagtt
     2341 tacccgattg tcgcatccgg ataccgaaca acagaaaaac agcaagaaat tatggatgaa
     2401 aaaattgccg aatataaggc gaaaggctac acctctgcac aggctaaagc ggaagcagaa
     2461 acttgggtgg ccgtgccggg aacgagcgag catcagcttg gtcttgctgt ggatatcaat
     2521 gcggacggaa ttcattcaac aggcaacgag gtttatagat ggctggatga aaacagctat
     2581 cgttttggtt ttattcgtcg ctacccacca gacaagacag agataaccgg tgtgagcaac
     2641 gagccgtggc attaccgata tgttggcatc gaagctgcca cagagatgta caaccaaggg
     2701 gtttgccttg aggaatattt aaaaccagaa aaatgagaaa aggatgttat gttatggaca
     2761 gaaaaagatt gacacagcgt ttcccgttcc tgcttccaat gagacgggcg cagagaaaaa
     2821 tgtgctttta tgcgggaatg agatttgacg gctgtcgcta tgcgcagacg ataggggaaa
     2881 aatcgctttc ccatttgctc tttgaaacgg attgcgcatt atacaaccac aataccggat
     2941 ttgacatgat ataccaggaa aacaaggtgt tcaacttaaa gctggcagca aagaccttaa
     3001 acggcttatt gataagaccg ggggaaccct tttctttctg gtggctggta cgccatgcgg
     3061 acaaagatac cccctataaa gacggcctta cggtgaccaa tggtaaactc accaccatgt
     3121 cgggcggcgg tatgtgccag atgagcaatt tactattttg gatgttcctg catacgccat
     3181 tgacaattat ccagcgcaga ggtcacgaag taaaggagtt tccagagcca aacagtgacg
     3241 agattaaagg ggtggatgca accatttcag agggctggat tgatttaaaa gtgcgaaacg
     3301 ataccgactg cacctaccaa atatgggtga ccttagatga tgagaaaatc atcggtcagg
     3361 tgtccgccga caaagagccc caagcattat acaaaattac aaatggcagt atccagtatg
     3421 tccgtgaaag tggcgggatt tatgaatatg cccaggttaa acggatgcaa gttgccttag
     3481 gtaccgggga aataatagat tgcaagctgc tttatacaaa caaatgcaaa atctgttatc
     3541 ccctcccgga aagtgtggat attcaggagg agaaccaatg agaaaaagtg tgggcattac
     3601 tgtttttgga tgcgaacagg atgaggcaaa tgctttccgc gctttatcgc cggattttca
     3661 tattatccct acgctgatta gcgatgcgat atcggcagac aacgcaaaat tggccgctgg
     3721 caatcaatgc gttagcgtag gccataagtc cgaggtttcc gaggcgacaa ttcttgcgct
     3781 gagaaaggtc ggggtaaaat acatttctac ccgcagcatc ggctgcgatc acattgatac
     3841 gactgccgcc gagagaatgg gaatctcggt tggcacggtt gcgtattcgc cggacagcgt
     3901 tgcggattat gctttgatgc tgatgctgat ggccatacgg ggtgcaaaac ccaccatgca
     3961 cgccgtggcg caacaagatt tcagattgga tcgtatccgg gggaaagaac tgggggatat
     4021 gactgtggga gttattggaa ccggccatat cgggcaagcg gtcgtcaaaa ggctgcgggg
     4081 atttggatgc catgtgctgg cctatgataa cagccgaaaa atggatgcag attatgtcca
     4141 gcttgatgag cttctaaaaa acagcgatat tgttacgctc catgtgccgc tttgtgcgga
     4201 tacccgccat ctgatcggtc agaagcaaat tggagagatg aagcaaggcg catttttaat
     4261 caacaccggg cgcggagcgc ttgtcgatac cggggcgctg gtggaggcgt tggaaagcgg
     4321 aaagctgggt ggtgcggcac tggatgtgtt ggagggcgag gatcagtttg tttataccga
     4381 ttgctcgcag aaagtgcttg accatccctt tttgtcgcag cttttaagga tgccgaatgt
     4441 gatcatcaca ccccatacgg cgtactacac tgagcgtgtg ctgcaggata ctacagaaaa
     4501 aacaatcagg aattgtctca attttgaaag gagtttacag catgaataga ataaaagtcg
     4561 caatcatctt cggcggttgc tcggaggaac atgatgtgtc ggtaaaatcc gcaatagaaa
     4621 ttgctgcgaa cattgatacg gaaaaattcg atccgcacta catcggaatt acaaaaaacg
     4681 gtgtatggaa gctatgcaag aagccatgta cggaatggga agccgacagt ctccccgcca
     4741 tactctcccc ggataggaaa acgcatgggc tgcttgtcat gaaagaaagc gaatacgaaa
     4801 cacggcgtat tgatgtggct ttcccggttt tgcatggcaa atgcggggag gatggtgcga
     4861 tacaggggct gtttgtattg tctggtatcc cctatgtggg ctgtgatatt caaagctccg
     4921 cagcttgcat ggacaaatca ctggcctaca ttcttacaaa aaatgcgggc atcgccgttc
     4981 ccgaatttca aatgattgat aaaggtgaca agccggaggc gggtgcgctt acctaccctg
     5041 tctttgtgaa gccggcacgg tcaggttcgt cctttggcgt aaccaaagta aacggtacgg
     5101 aagaacttaa cgctgcgata gaagcggcag gacaatatga tggaaaaatc ttaattgagc
     5161 aagcgatttc gggctgtgag gtcgggtgtg cggtcatggg gaacgaggat gatttgattg
     5221 tcggcgaagt ggatcaaatc cggctgagcc acggtacctt ccgcatccat caggaaaacg
     5281 agccggaaaa aggctcagaa aatgcgatga ttacagttcc cgcagacatt ccggtcgagg
     5341 aacgaaatcg ggtgcaggaa acggcaaaga aagtatatcg ggtgcttgga tgcagagggc
     5401 ttgcccgtgt tgatcttttt ttgcaggagg atggcggcat cgttctaaat gaggtcaata
     5461 ccctgcccgg ttttacatcg tacagccgct acccacgtat gatggccgcc gcaggaatca
     5521 cgcttcctgc actgattgac agcctgatta cattggcgtt aaagaggtga taagtatgga
     5581 aaatggtttt ttgtttttag atgaaatgtt gcatggcgtc cgttgggatg ccaagtacgc
     5641 cacatgggat aacttcacag gaaaaccggt ggatgggtat gaggtgaatc gcatcatcgg
     5701 cacaaaggcc gtggcgtttg ctctgcgcga ggcacaaatc catgcggctg cgcttggcta
     5761 tggcttgctt ttatgggatg gatatcggcc aagaactgcg gtggactgct tcctgcgttg
     5821 ggcagcgcaa ccggaggaca atctcacaaa agaaaaattt taccccaata tagagcgagc
     5881 cgagttgatt acaaagggtt atgtggcctc acaatccagc catagccgtg gaagcgcaat
     5941 tgatcttacg ctctaccacc tggatacagg ggaacttgtt tcaatgggaa gtaacttcga
     6001 ttttatggac gaacggtcgc atcatacagc aaaagggata ggggatgcag aggcacaaaa
     6061 tcgaagatgc ttgcgtaaaa tcatggaaag cagcggattt cagtcttatc gctttgaatg
     6121 gtggcactat aagttgattg atgagccata ccccgatacc tattttaatt ttgccgtttc
     6181 ataatgaaag tattggtttt gtatcagatt ttggatttga taaaatctat gctggtcatg
     6241 gacaatcttt tactggaaag tggttaaagc atcttttgta atacattgca aattatagta
     6301 ttcaatctgc ccagcggcag aaaagaaaaa aaccgctgtt gggcagacct ttcccacgcc
     6361 cattttgtat tttgctggcg gcggttttga gaaatcaagg ccgccgttct tttctggcag
     6421 caaaaggtgt ggcggcccat aggaggatgc cgctatgctg ttttgctatt gccatgctct
     6481 aatccacttc caccactcaa aaaaagcctt gcccctccat gaactgaacc ccaaaagttg
     6541 aactatttaa tggactgt
//
LOCUS       DANKHL010000413         6558 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.guided.163, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000413 DANKHL010000000
VERSION     DANKHL010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.guided.163"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            <1..413
                     /locus_tag="PW101_003076"
     CDS             <1..413
                     /locus_tag="PW101_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2515412.1"
                     /translation="DDENQIRAFDAEADDYVTKPFKMQILLKRVEALLRRSGALAKEI
                     RVGRLTLLPEDFTVLCDGTELPLTRKEFEILLLLVQNKGRTLTHEIILSRIWGYDFEG
                     DGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEES"
     gene            416..1759
                     /gene="vanS"
                     /locus_tag="PW101_003077"
     CDS             416..1759
                     /gene="vanS"
                     /locus_tag="PW101_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDL2515413.1"
                     /translation="MERKGIFIKVFSYTIIALLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHGMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQILAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQAQSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            1930..2736
                     /gene="vanY"
                     /locus_tag="PW101_003078"
     CDS             1930..2736
                     /gene="vanY"
                     /locus_tag="PW101_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HDL2515414.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            2754..3581
                     /gene="vanW"
                     /locus_tag="PW101_003079"
     CDS             2754..3581
                     /gene="vanW"
                     /locus_tag="PW101_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HDL2515415.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGEPFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            3578..4549
                     /locus_tag="PW101_003080"
     CDS             3578..4549
                     /locus_tag="PW101_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HDL2515416.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            4542..5570
                     /gene="vanB"
                     /locus_tag="PW101_003081"
     CDS             4542..5570
                     /gene="vanB"
                     /locus_tag="PW101_003081"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HDL2515417.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGTFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            5576..6184
                     /locus_tag="PW101_003082"
     CDS             5576..6184
                     /locus_tag="PW101_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HDL2515418.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(6250..6423)
                     /locus_tag="PW101_003083"
     CDS             complement(6250..6423)
                     /locus_tag="PW101_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515419.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1856 a         1384 c         1722 g         1596 t
ORIGIN      
        1 cggatgacga aaaccaaatc cgggcgtttg atgcagaggc agacgactat gtaacaaagc
       61 cattcaagat gcagatttta ctaaagcggg tggaagccct gctgcggcgc agcggtgcgc
      121 tggcaaagga aatccgtgtc ggcaggctga cacttctgcc ggaggatttc acggtacttt
      181 gtgacggtac agagctgccc ctgacacgaa aagaatttga aatccttttg ctgctggtgc
      241 agaacaaagg cagaacctta acgcatgaaa tcattttgtc acgtatatgg ggatatgact
      301 ttgagggtga tggcagcaca gtccacactc atatcaaaaa tttgcgggcc aagctgccgg
      361 aaaatatcat caaaaccatt cgcggtgtag gataccgatt ggaggaatca taataatgga
      421 aagaaaaggg attttcatta aggttttttc ctatacgatc attgccctgc tactgcttgt
      481 cggtgtaaca gcaacactgt ttgcacagca atttgtgtct tatttcagag tgatggaatt
      541 acagcaaaca gtaaaatcct atcagccatt ggtggaactg attcagaata gcgataggct
      601 tgatattcaa gaggtggcag ggctgtttca ctacaataac caatcctttg agttttatat
      661 tgaagataaa gagggaagcg tactctatgc cacaccaaat gccaatacat caaatagttt
      721 tagacccgac tttctttatg tggtacatag agatgataat atttcgattg ttgctcaaag
      781 caaggcaggt gtgggattgc tttatcaagg gctgacaatt cggggaattg ttatgattgc
      841 gataatggtt gtattcagcc ttttatgcgc gtatatcttt gcgcggcaaa tgacaacgcc
      901 gatcaaagcc ttagcggaca gtgcgaataa aatggcaaac ctgaaagatg taccgccgcc
      961 gctggagcga aaggatgagc ttggcgcact ggctcatggt atgcattcca tgtatgtcag
     1021 gctgaaagaa accatcgcaa ggctggagga tgaaatcgca agggaacatg agttggagga
     1081 aacacagcga tatttctttg cggcagcttc tcatgagcta aaaacgccca tcgcggctac
     1141 aagcgttctg ttggagggaa tgcttgaaaa tatcggtgac tacaaagatc attctaagta
     1201 tctgcgcgaa tgcatcaaaa tgatggatag gcagggcaaa atcatttccg aaatactgga
     1261 gcttgtcagc ctgaatgatg ggagaatcgt acccatagct gaaccgttgg acatagggcg
     1321 cacggttgcc gagttgctgc ccgattttca aatcttggca gaggcaaaca accagcggtt
     1381 cgtcacagat attccagccg ggcaaattgt cctgtccgat ccgaggctgc tccaaaaggc
     1441 actatccaat gtcatattga atgcagttca gaacacgccg cagggaggcg aggtacggat
     1501 atggagtgag cctggtgctg aaaaatgccg cctttttgtt ttgaacatgg gcgttcacat
     1561 tgatgatact gcgcttccaa ggctgttcac cccattctat cgcattgatc aggcgcaaag
     1621 cagaaaaagt gggcgaagcg gtttaggact tgccatcgta caaaaaacgc tggatgccat
     1681 gagcctccag tatgcgctgg aaaacacctc ggatggcgtt ttgttctggc tggatttacc
     1741 gctcacatca acattgtaaa tatttaaaac ttaaatgatt ttgaccgaca ggtataaccc
     1801 tgccggtctt tttttgctgc tacaggaaaa ctacagactg gctacaggga aagtacagat
     1861 gcgcttgcca taataacaat cgtaccagcc acaaatcgta gttttattgc aaaggaggca
     1921 ttcgatcaaa tggaaaaaag caactatcat tccaatgcgg atcatcacaa acggcatatg
     1981 aaacaatccg tggaaaagcg ggcttttcta tgtgcgttca ttatttcgtt cacagtctgc
     2041 acgctgtttt tggggcggag actggcttcc gtattggagg caacacagat accgcccatc
     2101 cctgcaactc atacaggcag cagcactgac gtagtggaga atttggagga aaacgctctt
     2161 gccaccgcca aagaacaggg agatgaacag gaatggagcc tgattttagt gaacaggcag
     2221 aaccccatcc ccgcacagta cgatgtggag cttgagcaac tatcaaatgg tgagcggata
     2281 gatattcgga tttctcccta tcttcaagat ttgtttgatg ccgcaagaac tgatggagtt
     2341 tacccgattg tcgcatccgg ataccgaaca acagaaaaac agcaagaaat tatggatgaa
     2401 aaaattgccg aatataaggc gaaaggctac acctctgcac aggctaaagc ggaagcagaa
     2461 acttgggtgg ccgtgccggg aacgagcgag catcagcttg gtcttgctgt ggatatcaat
     2521 gcggacggaa ttcattcaac aggcaacgag gtttatagat ggctggatga aaacagctat
     2581 cgttttggtt ttattcgtcg ctacccacca gacaagacag agataaccgg tgtgagcaac
     2641 gagccgtggc attaccgata tgttggcatc gaagctgcca cagagatgta caaccaaggg
     2701 gtttgccttg aggaatattt aaaaccagaa aaatgagaaa aggatgttat gttatggaca
     2761 gaaaaagatt gacacagcgt ttcccgttcc tgcttccaat gagacgggcg cagagaaaaa
     2821 tgtgctttta tgcgggaatg agatttgacg gctgtcgcta tgcgcagacg ataggggaaa
     2881 aatcgctttc ccatttgctc tttgaaacgg attgcgcatt atacaaccac aataccggat
     2941 ttgacatgat ataccaggaa aacaaggtgt tcaacttaaa gctggcagca aagaccttaa
     3001 acggcttatt gataagaccg ggggaaccct tttctttctg gtggctggta cgccatgcgg
     3061 acaaagatac cccctataaa gacggcctta cggtgaccaa tggtaaactc accaccatgt
     3121 cgggcggcgg tatgtgccag atgagcaatt tactattttg gatgttcctg catacgccat
     3181 tgacaattat ccagcgcaga ggtcacgaag taaaggagtt tccagagcca aacagtgacg
     3241 agattaaagg ggtggatgca accatttcag agggctggat tgatttaaaa gtgcgaaacg
     3301 ataccgactg cacctaccaa atatgggtga ccttagatga tgagaaaatc atcggtcagg
     3361 tgtccgccga caaagagccc caagcattat acaaaattac aaatggcagt atccagtatg
     3421 tccgtgaaag tggcgggatt tatgaatatg cccaggttaa acggatgcaa gttgccttag
     3481 gtaccgggga aataatagat tgcaagctgc tttatacaaa caaatgcaaa atctgttatc
     3541 ccctcccgga aagtgtggat attcaggagg agaaccaatg agaaaaagtg tgggcattac
     3601 tgtttttgga tgcgaacagg atgaggcaaa tgctttccgc gctttatcgc cggattttca
     3661 tattatccct acgctgatta gcgatgcgat atcggcagac aacgcaaaat tggccgctgg
     3721 caatcaatgc gttagcgtag gccataagtc cgaggtttcc gaggcgacaa ttcttgcgct
     3781 gagaaaggtc ggggtaaaat acatttctac ccgcagcatc ggctgcgatc acattgatac
     3841 gactgccgcc gagagaatgg gaatctcggt tggcacggtt gcgtattcgc cggacagcgt
     3901 tgcggattat gctttgatgc tgatgctgat ggccatacgg ggtgcaaaac ccaccatgca
     3961 cgccgtggcg caacaagatt tcagattgga tcgtatccgg gggaaagaac tgggggatat
     4021 gactgtggga gttattggaa ccggccatat cgggcaagcg gtcgtcaaaa ggctgcgggg
     4081 atttggatgc catgtgctgg cctatgataa cagccgaaaa atggatgcag attatgtcca
     4141 gcttgatgag cttctaaaaa acagcgatat tgttacgctc catgtgccgc tttgtgcgga
     4201 tacccgccat ctgatcggtc agaagcaaat tggagagatg aagcaaggcg catttttaat
     4261 caacaccggg cgcggagcgc ttgtcgatac cggggcgctg gtggaggcgt tggaaagcgg
     4321 aaagctgggt ggtgcggcac tggatgtgtt ggagggcgag gatcagtttg tttataccga
     4381 ttgctcgcag aaagtgcttg accatccctt tttgtcgcag cttttaagga tgccgaatgt
     4441 gatcatcaca ccccatacgg cgtactacac tgagcgtgtg ctgcaggata ctacagaaaa
     4501 aacaatcagg aattgtctca attttgaaag gagtttacag catgaataga ataaaagtcg
     4561 caatcatctt cggcggttgc tcggaggaac atgatgtgtc ggtaaaatcc gcaatagaaa
     4621 ttgctgcgaa cattgatacg gaaaaattcg atccgcacta catcggaatt acaaaaaacg
     4681 gtgtatggaa gctatgcaag aagccatgta cggaatggga agccgacagt ctccccgcca
     4741 tactctcccc ggataggaaa acgcatgggc tgcttgtcat gaaagaaagc gaatacgaaa
     4801 cacggcgtat tgatgtggct ttcccggttt tgcatggcaa atgcggggag gatggtgcga
     4861 tacaggggct gtttgtattg tctggtatcc cctatgtggg ctgtgatatt caaagctccg
     4921 cagcttgcat ggacaaatca ctggcctaca ttcttacaaa aaatgcgggc atcgccgttc
     4981 ccgaatttca aatgattgat aaaggtgaca agccggaggc gggtgcgctt acctaccctg
     5041 tctttgtgaa gccggcacgg tcaggttcgt cctttggcgt aaccaaagta aacggtacgg
     5101 aagaacttaa cgctgcgata gaagcggcag gacaatatga tggaaaaatc ttaattgagc
     5161 aagcgatttc gggctgtgag gtcgggtgtg cggtcatggg gaacgaggat gatttgattg
     5221 tcggcgaagt ggatcaaatc cggctgagcc acggtacctt ccgcatccat caggaaaacg
     5281 agccggaaaa aggctcagaa aatgcgatga ttacagttcc cgcagacatt ccggtcgagg
     5341 aacgaaatcg ggtgcaggaa acggcaaaga aagtatatcg ggtgcttgga tgcagagggc
     5401 ttgcccgtgt tgatcttttt ttgcaggagg atggcggcat cgttctaaat gaggtcaata
     5461 ccctgcccgg ttttacatcg tacagccgct acccacgtat gatggccgcc gcaggaatca
     5521 cgcttcctgc actgattgac agcctgatta cattggcgtt aaagaggtga taagtatgga
     5581 aaatggtttt ttgtttttag atgaaatgtt gcatggcgtc cgttgggatg ccaagtacgc
     5641 cacatgggat aacttcacag gaaaaccggt ggatgggtat gaggtgaatc gcatcatcgg
     5701 cacaaaggcc gtggcgtttg ctctgcgcga ggcacaaatc catgcggctg cgcttggcta
     5761 tggcttgctt ttatgggatg gatatcggcc aagaactgcg gtggactgct tcctgcgttg
     5821 ggcagcgcaa ccggaggaca atctcacaaa agaaaaattt taccccaata tagagcgagc
     5881 cgagttgatt acaaagggtt atgtggcctc acaatccagc catagccgtg gaagcgcaat
     5941 tgatcttacg ctctaccacc tggatacagg ggaacttgtt tcaatgggaa gtaacttcga
     6001 ttttatggac gaacggtcgc atcatacagc aaaagggata ggggatgcag aggcacaaaa
     6061 tcgaagatgc ttgcgtaaaa tcatggaaag cagcggattt cagtcttatc gctttgaatg
     6121 gtggcactat aagttgattg atgagccata ccccgatacc tattttaatt ttgccgtttc
     6181 ataatgaaag tattggtttt gtatcagatt ttggatttga taaaatctat gctggtcatg
     6241 gacaatcttt tactggaaag tggttaaagc atcttttgta atacattgca aattatagta
     6301 ttcaatctgc ccagcggcag aaaagaaaaa aaccgctgtt gggcagacct ttcccacgcc
     6361 cattttgtat tttgctggcg gcggttttga gaaatcaagg ccgccgttct tttctggcag
     6421 caaaaggtgt ggcggcccat aggaggatgc cgctatgctg ttttgctatt gccatgctct
     6481 aatccacttc caccactcaa aaaaagcctt gcccctccat gaactgaacc ccaaaagttg
     6541 aactatttaa tggactgt
//
LOCUS       DANKHL010000414         5770 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.guided.186, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000414 DANKHL010000000
VERSION     DANKHL010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.guided.186"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            322..1014
                     /locus_tag="PW101_003084"
     CDS             322..1014
                     /locus_tag="PW101_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL2515420.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1341..1838
                     /gene="dfrG"
                     /locus_tag="PW101_003085"
     CDS             1341..1838
                     /gene="dfrG"
                     /locus_tag="PW101_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDL2515421.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1910..3862)
                     /locus_tag="PW101_003086"
     CDS             complement(1910..3862)
                     /locus_tag="PW101_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDL2515422.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3869..4105)
                     /locus_tag="PW101_003087"
     CDS             complement(3869..4105)
                     /locus_tag="PW101_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515423.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4507..4797
                     /locus_tag="PW101_003088"
     CDS             4507..4797
                     /locus_tag="PW101_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2515424.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT         1967 a          920 c          995 g         1888 t
ORIGIN      
        1 tttcataggt aaaagaagca tgacaattta tgacaaatcc gcgttttttg taataaatac
       61 agtaatctat gaaatgaaaa gggttacgtt taataaaact tgcttttttg tttttgttca
      121 ccataaatat ttatttaaga aagtattaac ctgcattttt ctctataaaa aagatttttt
      181 aaatagataa aaaaggagga ttaaatgagt atttgatagt atttatatta aaattacata
      241 tgtaatttaa aaacgcttga cattgtgttt gagatcactt acttttagca tgtaaaagta
      301 ttggattaag gagctatgca aatgaaaaag aaatttatta ctggtgggat tgtcgttgca
      361 tttctatgtc taattggcta cacaggcacg cggtacgcaa atgaaattat tttgtggggt
      421 ggagagacag atattgatac aatcaatgag aatctgacac agcttgatca tacattgaca
      481 gataaagagc aagcgattac agatcttacc gctcgactag cacaaaaaga acagacattg
      541 caagattcaa aggcagatgt ccaagaatac caaacaaaag taaatgaatt agaaaaccaa
      601 aaaaatcaat cggcagcaga aaaacaaaac ttagaaagtc aattagtcag caaggatgca
      661 caactacaaa gcaaacaaaa tgaaatcaat gtcaaactag atgagatcaa ccaaaaaaat
      721 gatgaaatca atcatctgaa tgcagattgg acagcaagac tacaagaagc acaagcaaaa
      781 ctttcagaat tacaaaatac tgtaaatggt ttgaatcaac aaatcgcaac actgacacaa
      841 gaaaaaacaa atgtagaaac tcagttaaca gatacacaaa agaaattaga cgaagcaact
      901 gctgaaaact caagcttgaa acaatatatt caaaagttag aaaaagcaaa agatgaagtg
      961 gaagatacag ctgataaatc tcagcaaatt gttgaagaac atttgcgtaa ataatctttc
     1021 ttaacaagta aacctaaatt agtaaagaag caacagtaat ctatactaag tagttcagtt
     1081 ttggagtaca aaatgacaaa atgtacttca aaatggacta cttttttcat tttaacttac
     1141 ataccaaatt acatacaaaa tgatttataa aatgcaattg aaaatgataa ttgaatgaaa
     1201 atttgtgatt ttgtggtatt atgataatat aacttataga aaaaattgct tattcaacta
     1261 acgggcagga taatgagaaa aggttttttg atagaaataa gaaattagat ggtaaattag
     1321 ttaaaaaggc tgtgttataa atgaaagttt ctttgattgc tgcgatggat aagaatagag
     1381 tgattggcaa agagaatgac attccttgga ggattcccaa ggactgggaa tatgttaaaa
     1441 atactacaaa gggacatccg ataatattag gtaggaagaa ccttgaatca atcggaagag
     1501 ccttacctga cagaagaaat attattctga cgagagataa ggggtttacc tttaatggtt
     1561 gtgaaattgt tcattcaata gaagatgttt ttgagttatg taaaaacgaa gaagaaattt
     1621 ttattttcgg aggagaacag atttataatt tgtttttccc ttatgttgag aaaatgtaca
     1681 tcacaaaaat acatcatgaa ttcgaaggag atactttttt tccagaagtg aattatgagg
     1741 aatggaatga ggtatttgcc caaaaaggga taaagaatga taaaaatccg tataactact
     1801 attttcatgt atatgaaaga aaaaacttat tgagttaacg ggtgcgttaa ttgaagaaga
     1861 acagtaaaga taaagcacaa gagcttggga ttactccaag ctctttttgt catttgaaat
     1921 aaattatatc tttccaatca aattgtttat ctgtaatccc taatctttga gcaggtgtca
     1981 aaaccttttt atccgctgat ttaaaagggc gacaaaagtt gtagtaagtt cttagaattg
     2041 taatcgcaaa ttgagcatac ttcggattga agttagcgta aatatagctc ttcttatcac
     2101 cacgagctgt tgttaatggt ctttcaagag atgatatata acgtcttatc tgttgaataa
     2161 agctattggt cgaatggtca ttaacattta atagcatctt tgcgatttcg tttggttcca
     2221 gagcagataa atccgttcta caatcaactg aataaaatcc tttatcctta gtggctaatg
     2281 gatgtttgat agggttatcg gcatatttgc gatatctttc tccatcttta ctaatagcct
     2341 cttcgtggaa ttgatgactt gttagtaatt cagttagata atgagatgcc aaagtccgta
     2401 gaaactttgt tttgaatcct ctgatatctc cccaatcgag cagttctgct tttgcttgtc
     2461 cgaattcttt taagcattgt tttctgcttt tcgtcttgtt aacttggcaa ataaaatgat
     2521 gtgcatcgct caaccttaat tctttcgtga atactctata aaaggcgttt ctaatagaga
     2581 agtcatcatc actaatcatt cgccattcgt cggcattaac catttgttta attaaccaat
     2641 agtgagcagt tgtagtataa ggagcatcaa catttaaacc atcgacaaat ctactcctat
     2701 tagtaatttt ccctaattcg tgaagataag cattcctatt ttcgctatcg ttagcggatg
     2761 gttcttgtgg ataataagac caatctaacc tatcattttt acgactaaaa gtattgagat
     2821 ggtcttcttt gaattttctt gtatcttcat ttaattcatc cattgaaata ttccaatcat
     2881 aggcaacatc agaacggaac acatatcttg ataagacctc agcagataca actatatacg
     2941 tttgcatatt aaggtcttca aaccccgtat atttctttga accttgtcct ttcttacgga
     3001 cgttatttag gtagtagtgc atcttgtccg tattaagcca catctcgtta aatttctttg
     3061 tttgaagggg ttgtgtctca tatcgttcta aaaactccaa acaacgccta tacagccact
     3121 ccagcttatg atagtaggtt ccaactccaa ttcctaaaat atcacaagta cgactgactg
     3181 aaacccttcc aacaaccatc ttagcgaaca ttggaagaat ggtgttcttt tgttggtgat
     3241 aagtagtggt ttcttctctt tttggtagaa cattagtgaa cttcttacac gccttacatt
     3301 gatagcgttg agatttgccc ctacttttcc cccttttgta aaattgcttc ggctcattga
     3361 atggggttga ttcttcttcc gagcagcctt ctttatgaaa ttgataatct ggctcaacat
     3421 cttggatgct attgatttct ataaggcgtt taatttcttc aacaacagac caattagata
     3481 aaggggttac tgtacagttt tgcgatacac ctctgccaat aggattgtca ttgcagtaca
     3541 tacctttatg acctttatct ttggaactac cagtcatttt atatcgactt ggtttccctt
     3601 ttacatcttt gaatttgtgc tgttctttcc caaaattaca acaaaacgga ttgatgcagt
     3661 aattatattg aatgggatat tcagaaccgt tccatttaaa agacgttggc aagaacaaca
     3721 ttttgttata ccttgttgca aatcgttttg gagttaataa atcatattgt ttagccctat
     3781 cttttaattc ttcgtttgaa atcggaattt taacatctac aatttcatct tccttcgtgg
     3841 ctaatctttt aagcttggtc attgtctatc aacccactct atctttgata cccatttttt
     3901 caacaatccg cttattactt cgcttgttgg caatccattc aataaacttc ttatcgtcta
     3961 aaatgttcaa taagaattca tccactgctt cgctttcagt acgctctgca tattcagcgt
     4021 attgcttaac tatcaccctt acttgctctg atatttccca atccacagac ttagcgttct
     4081 tattttttgg ctcaagaaat tgcataggaa aatgcacccc tttttgctta gtaaattgta
     4141 accattttaa cacaccaatt tgcattccta caataattat ttcacgttcg catttttata
     4201 tacattttaa cttacatttt gtggtgtaaa ttaacaacaa acgttgattt aacaataaaa
     4261 aacgagtatc aaattcagtt gcaatttgat actcatttag gtttacattt tcactttttg
     4321 tactccaaaa ctgaactact taaatctata ctgctgcctc ttttttttag tacatgtcct
     4381 tcatcttcgc aggaagtgcc aatatgattt taagaaaggt aatagacaaa aagtcaaaaa
     4441 gataaaacag agtttcttct ttacaaaggg aatgtatgtt cgtataatgg tattgaggtg
     4501 aaggttatgg agaaaaaata tggaacagta gataagatcc gtactttgca agttagtgcc
     4561 agaccgttga tccgtttcag tttaaatggg gtcaattgtt tgattgcttc ccatagtttg
     4621 aacttcctag ctgaagtaga cgaagggatg aagttagtcg ttacaggtta ttataataat
     4681 agaaagcaat ttgttgtgag atcctatcat gtgttaggga aacccaaaat tgtggtagaa
     4741 tatgagaaaa gtctataccc aagaaaaaag gtccgacaag atgaaagaat aaactagaaa
     4801 acaagcggct gagacaaaaa tgtcctagcc gcttgttttg gaaagatgga tgcagacgat
     4861 gggatttgaa cccacacgag catacgctca caagaacctc aatctatata taactacatg
     4921 tatttaagta tttgtgatga atcactaaac tttccaaact catttgaggg atattgctta
     4981 aagattgaac agatcaattc gaagccctat tagtattcgc tcaatggggg gatcagaggc
     5041 cataaaaaga gactagtcat tactagtctc aggtagtgga attgtaggat tttaaacgtt
     5101 cctggagagt accggtatgt aactcgaaga ggtgattatc atagtcgtaa aagtaaattg
     5161 aagaagcctc gcctttcaca cgagaacgtc ctggttcgac agttaaacct aacatttgga
     5221 tttttgagac aaacgaatct atatcttgtt cgtcaatttt aaaagcaata tgattgtatg
     5281 tctttggcag tttattcgac gaattctgca ttactgcaat ccataggtcc ttaactaaga
     5341 aaaatttttc tggatataat gaaaattttt ttgttgacta tcgtagattt cttttgcatc
     5401 aaataaggta tttaacaatt aagtatttaa aagataactt ttaagttatc tttaagtaat
     5461 gcttaaagct atgttatact ctattaggaa atataagaag gagtataaaa atgaacaaac
     5521 ataatagaaa aataacgaat aacaaagaac tgatcttgtt actaataggg atttggggta
     5581 tctttttact ctttaatatg tatatgccga ctatgagagc ggatgacgta gtttatgcaa
     5641 gtagattaga tgaacttggc tatttgggtg cttcgataga acattataag acatggagtt
     5701 caagaattat tatagaacta ttcttaatgt tcttttctaa acactttatg ctatggaaat
     5761 tattaaattc
//
LOCUS       DANKHL010000415         4129 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.guided.228, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000415 DANKHL010000000
VERSION     DANKHL010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.guided.228"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            79..1557
                     /gene="msr(C)"
                     /locus_tag="PW101_003089"
     CDS             79..1557
                     /gene="msr(C)"
                     /locus_tag="PW101_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL2515425.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1604..2050
                     /locus_tag="PW101_003090"
     CDS             1604..2050
                     /locus_tag="PW101_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL2515426.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2249..2764)
                     /locus_tag="PW101_003091"
     CDS             complement(2249..2764)
                     /locus_tag="PW101_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2515427.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            2881..4083
                     /locus_tag="PW101_003092"
     CDS             2881..4083
                     /locus_tag="PW101_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2515428.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1227 a          704 c          894 g         1304 t
ORIGIN      
        1 tttttttgca aacagatttt tctgctcttt tttagtatcg ataattgttg ttttacatga
       61 aataggagtg taaaacatat ggaaaattta gcagtaaata taacaaatct acaagtcagc
      121 tttggcaacc agctagaact atctattgat tctcttcgtg tctatcagca agatcgtata
      181 gggatcatag gagaaaatgg tgtcggtaaa tcgactttgc tcaaactaat agccggtgaa
      241 ctttttcccg atcatgggaa aatccaaaca gagatcacct tcaactacct gcctcaatta
      301 acctatctcg ctgaagcaaa ggacctaaat ttggaattag ccagtcattt ccagttaaga
      361 ctggaagaaa cttcagagcg gaaatggagc ggaggggaag aacgaaagat cgagttgata
      421 cgtcttcttt cttcttatga acaagggatg cttctagacg agccaacaac ccatctagat
      481 agaaaaagta ttgatcgact gattgaagag cttcgttatt attatggtac gctggttttt
      541 gttagtcatg atcgctattt tctagatgag ctagcatcga aaatctggga agtaaaagac
      601 ggagaaatcc gagagttttc ggggaattat agtgcctatc tcactcaaaa ggaattggag
      661 aaaaagactc agctacgaga agcagagtcg atcatgaaag agaaaaaacg attggaaaaa
      721 tcgatccaag aaaagaaaaa acaagcggaa aagttggaaa aagtgtccag taaaaagaaa
      781 aagcaacaaa tcagaccgga tcggttgtct tcctctaaac aaaaagacag tgtacaaaaa
      841 gccatccaaa agaatgcgaa aacattagag agaagactcc aaaaaatagg agaaacaacc
      901 aagccgcagc agatgaaaca aatccgtttt ccagtaccaa aatctcttga actccacagc
      961 cgctatccca tcatgggaca aaatgtccaa ttggaaagaa gtgggagaac attactggta
     1021 aatggtgatt ttcagttttc tttaggtaaa aaaatcgcga ttgtcggtga aaatggttca
     1081 ggtaagacaa ccttattaga acatatccgc aaacaaggag aaggaatcct tctctctccg
     1141 aaagtaagct ttcaagtata tcagcaaaag ggttatcaaa tgacatctga agaatccatc
     1201 attcgttttg tcatgagaca aacagagttt tcagaatcgc ttgtccgtag tttgctgaat
     1261 cacttagggt ttgctcagga aactttgacg aaaccgttat gtacattaag tgggggagaa
     1321 gcgacccgtc tgacgattgc tttgcttttt actaagccaa gtaatgtgtt gctgttagat
     1381 gaaccgacta attttattga tatggcaacg atcgaagctt tagagaagct aatgcaaata
     1441 tatccgggaa cgattttgtt tacttctcat gattcttact ttgtcgagcg tacggctgat
     1501 gaagtttatg aaataaaagg gcagaaaata aaaaaagtac ttacgagaaa tttttaaatg
     1561 tgctacgatg attttcgtga caaatttaaa ggagtggtac agattgccta caaataaaaa
     1621 agaaggaata atttttacaa caatgatgtg ttttttgatg gtcttgggga tgagcgcgta
     1681 taatttatgg ctgcatcaag atcttcattt atcaagtttg ttgattggtc tagtgcctgg
     1741 attcgtggtg gctttcattt tggatgtctt catcatcggt gtgctggcga aaaaaattgc
     1801 ctttaaacta ccagtaaatc cagaaagcaa ggtccaaatg atcttaacta tttcatgctt
     1861 gatggtttta ggaatggtga catgtatgtc tttattcggt gtgatcatgg aacaaggaat
     1921 acctgatcaa ttattctctg cttatatgaa tgcttggaaa atgaatgtga ttgccgcttt
     1981 accgttgcag ttgatcatcg ttggcccgct ttcaagaagt gtgctaaaag caattcaaag
     2041 taatgaatga tggaacactg ttatttgaaa aaaggccttg gcatgctttt tgtcatagtc
     2101 tcttgaatgc agttctagaa aaatagtgat ccgaacgata ggttttggcc tgtcgttcgg
     2161 atcgcttttt cttttccaac tgtattgctc ttttattgaa gataatttgc tcttcaatag
     2221 atatttctag aaaggttctg tttatccctt actcaaaaaa ctgctgcatt tgttctctta
     2281 cttgctgaca ccattggagt tcctgctgac aaagcataat tctgcgctgg atcacaacat
     2341 aacgtccaaa ttcttgtttc ttgtctagcg agaggtcatt caattttttt tgattttttt
     2401 gcatgatttt gttcagctgt tgttctctag tgaatagcag tccagtagct gttgttttat
     2461 cgagcgtcga aaaagcatag atttttgcta gaaattcatc tttttgcgta ggagcgggag
     2521 tttcctcttt gatccattcc tctacaagag cacgtccttc atcagtcaag tggtagatct
     2581 ttttttgaga gtgctcgtct ttgtccaaaa cggtcacata accctgctgg tctaacttgg
     2641 caagcgttgt atagatctga ctgtgatggg cttcccaaaa aattgtgatc agctgcttca
     2701 attcataccc agaacgtggt ttggataaaa gcacgcataa taatatatag gctaaagtat
     2761 tcataaaaat cctacctctc gtcttatttt ctttattata tcacagtaaa gtttccacgt
     2821 tttacatgtg catatttaca tgtaaaataa atcatgtatg atgctaatag agagaaaggg
     2881 atggaaatat tggagaatca aatagatata caagaacaaa caaatgaaga aacaaagggg
     2941 aaaagttcag cgctagttgt agcagctacc tgtatgatcg cttttatggg agtgggtcta
     3001 gtagacccaa tcttaaaaac gattgctgca cagttaaacg ctacgccagc tgaaacgacc
     3061 ctgttattta ctagttacat gttagtcact ggtgtggtga tgctatttac aggtttctta
     3121 tctagtcgga tcggtttgaa aaaaacattg atgacaggat tgtttatcat cgttgtattc
     3181 gctggtcttg gcggattatc tgggaatatc ggaatgctga ttgggttacg tgccggctgg
     3241 ggtgtcggga atgctttatt tgtctctact gctttagcaa cgatcgtcag tattttagtt
     3301 ggtaacacag aaaaagcaat catgatgtat gaagcagcac ttggctgcgg aatggctgtt
     3361 ggccctctag tcggtggggt cttgggcagt ggttcatggc gttatccatt ttttggggta
     3421 gcatttttga tgttttgcgg ttttatagcc ctagcggtac ttttacctga aactgagaaa
     3481 ccaaagagaa aagtaggcct ttgggaaggt gtcaaagcgt taagcaatca taaacttcgt
     3541 tcagttggac taatcgcatt gttatataat ttcggattct ttactttact tgcgtatgcg
     3601 ccatttttat tgattggcta ttctgaatta gaagtcggtt ttgtattttt tggctggggt
     3661 gtgcttctgg ctattgcttc gattttcgtt gcaccggctc ttgagcggaa gtttacgact
     3721 tatcggacaa ttttagcagc atttgtctta ttcattattt gtttgctgtt attaggagct
     3781 ggaacttcta tgccagtcct tgtaccgatc agcattgtgt tagcaggatt tttccaagga
     3841 attatcaata cactgttgac gacgattgct atggaaatac caggtctgca acgaaacgtt
     3901 gcttcgtcca gctatagttt tgtccgcttt ttcggtggag ctttagcacc atttattgca
     3961 ggtgtgattg gtgaacatat tggtgaaagc tatgcgtttt attttgcagg tgccattgtt
     4021 cttatcagtg tatgttgtat catttatcac cgtaattatt ttgctacaaa tgaagaagaa
     4081 taagaaatca gaggttgtga aaaagctgtc ctgcatcaag tcataagaa
//
LOCUS       DANKHL010000416         2772 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538945-rid17233443.guided.270, whole genome shotgun
            sequence.
ACCESSION   DANKHL010000416 DANKHL010000000
VERSION     DANKHL010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538945
            Sequence Read Archive: ERR10688508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638262.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638262.1
            Genome Coverage       :: 367x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,097
            CDSs (total)                      :: 3,060
            Genes (coding)                    :: 2,996
            CDSs (with protein)               :: 2,996
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 13 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538945-rid17233443.guided.270"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-04-26"
     gene            111..194
                     /locus_tag="PW101_003093"
     CDS             111..194
                     /locus_tag="PW101_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HDL2515429.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            319..1056
                     /gene="erm(B)"
                     /locus_tag="PW101_003094"
     CDS             319..1056
                     /gene="erm(B)"
                     /locus_tag="PW101_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL2515430.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1319..2182)
                     /locus_tag="PW101_003095"
     CDS             complement(1319..2182)
                     /locus_tag="PW101_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2515431.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2184..2456)
                     /locus_tag="PW101_003096"
     CDS             complement(2184..2456)
                     /locus_tag="PW101_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2515432.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2473..2682)
                     /locus_tag="PW101_003097"
     CDS             complement(2473..2682)
                     /locus_tag="PW101_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDL2515433.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          851 a          488 c          443 g          990 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccaa aattttgtat aataggaatt
       61 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
      121 tccaaatgcg taatgtagat aaaacatcta ccgttttgaa acagactaaa aacagtgatt
      181 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      241 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta tagatgtaat
      301 cacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
      541 actcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      601 tataaaattg ttgggaatat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     1021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttgtaaatt
     1081 tggaaagtta cacgttacta aagggaatgt agataaatta ttaggtatag ggcacctcta
     1141 ataactacca attaattcac ctcaaaatga aattagaaaa aaacagcgaa atttttaaat
     1201 ctatttctta tcgatacaaa ttcctcgtag gcgctcggga ccccaacatg aggaaatgtt
     1261 tttataaaaa aagagtagtt accgtttttt ggtaactact cttttccttt tttaaggttt
     1321 aaatacctgg aagtttaggt gttttgggta ttggtggtgt aggtagctgc aagctttcca
     1381 ttttttgttg aattgcttta aattcagggg tttcttggtg ttgattttga accatttttt
     1441 gctctattct ctccaaagtc ggttgaattt ctttccctga tactttacga tttaattctc
     1501 tttctaaggt ctctttcgga ctaatggaag gcgtttctaa gcttgaatag agtttcactc
     1561 cttctctatt gtaaagcctt atatcgctaa ataagcccgt tttatgaagg gtttctaaat
     1621 tggtcggtaa gtttttgaca acaatatcat gcgcttgttt tggtgttgcc ctagctgtca
     1681 ttggatcatc tgcatacatg gtttcatatc gttcaatcgt tcctaagtat gattcaattt
     1741 tcggtactgc catgacgtat atttttgttt cataaccttt agattgaagc attgtggctg
     1801 tttgaatggg aacgtctgtt gttcgtcctg ttccttcaat cactaaatta tagccttgat
     1861 cgcttaaacg gctaataagt gcttctgtca tgcgattcga ataaggggta acgtgtttta
     1921 ctacgtcttt ttcatagagt ttcactaatt cgtcaaaatt tgggtgctgt tgtttaaagg
     1981 tatcattatc aatgacaata acattccctt gtgtttcttc aaaaattgct gatcgcaaac
     2041 tggttttccc tgaccctggt tgcccaccaa gtaaaaaagc ggttggcgat tcaaccgcta
     2101 gtctattttt agttagtcgt tcaacattct tttctaaacg atcttcaaat tgcttttcgg
     2161 tgaagtcagt aatattcgcc atgttaagcc actttctctt tattcaaaat ttgttttgaa
     2221 tattgattca ttgaccgcca atactcatga atagcttgta attcttcaaa tggttgttga
     2281 aataaatcaa cttcttgtgc cacttctaat tgatttaaaa gattcacttc tagtaaacga
     2341 gtatttttag tatctagttc atgattcaaa acataattta atacccgatt ataaacactt
     2401 gccgataact cattaatgat ttcaatttca aatgtttttt catacgtaac tgccatttca
     2461 ttttcactcc ttttaaagtt tgtcaggtaa atatttccgt atatattctt ctaaggcttg
     2521 atccataact tcttttacgt ttccgccatt ctttgctgtt tcgattttta tgatatggtg
     2581 caagtcggca cgaacacgaa ccgtcttatc ccccattata tctttttttg cgctgattgg
     2641 cgtatcattt cgttttgctt tctgtgcgcc taaatttccc acgatcgctc acttctttct
     2701 atttcttctt actatttatt ttatcatcac ctatcacaaa tcacaagtga tttgtgatta
     2761 atcacttgtg at
//
